LOCUS       DASUEV010000001         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1260182, whole genome shotgun sequence.
ACCESSION   DASUEV010000001 DASUEV010000000
VERSION     DASUEV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260182"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFV55_00005"
     CDS             <1..646
                     /locus_tag="VFV55_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HEX4942702.1"
                     /translation="IAGLGLGALLGSLFSGGLGGMGGILLLLLAAGVAFMAYRAFASR
                     RAPVTNPPMQFEGIGSDVPARPPLNIGGGINSAPVESPQSQAAAAAIPGFEAEPFLRV
                     AKTSFIRLQAANDARDLDDIRDYTTPEMYAEISMQIRERGDAIQKTEVVTLNASLVEA
                     VVEGDYAIASVRFTGLIREAADANPEPFDEIWHVRKSLVDRKATWLIAGIQQAS"
     gene            complement(662..898)
                     /locus_tag="VFV55_00010"
     CDS             complement(662..898)
                     /locus_tag="VFV55_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942703.1"
                     /translation="MKDFRDHFIEKALDKAVGAYRPDKSPRSRIARIAIIAALAFATF
                     VGFWTILHLSTPRPGPPVERKPITVELLPAPAKR"
     gene            928..1272
                     /locus_tag="VFV55_00015"
     CDS             928..1272
                     /locus_tag="VFV55_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942704.1"
                     /translation="MNADTPKSTRKAQLDALLLKLPGVSTKKINGLDAYFVSDRMFAC
                     ISGNGIGLRLPVAAATELQFSRENVVPFQPGGMASTREWIQIDRADAADYGKDLELFQ
                     ASIEFVKGGRTR"
     gene            1349..2317
                     /locus_tag="VFV55_00020"
     CDS             1349..2317
                     /locus_tag="VFV55_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4942705.1"
                     /translation="MNNRSTRMAFLAAIGTLSAFSALAQTVKVTPLGGIDSEFCPQDR
                     ALVFEDPNGTRILYDPGRTVAGADDPRLGKIDIILVSHMHGDHVGNAHNKAPNSGSCD
                     KPDVSVSAMPNSSAVNIALAKKAKIVTGSEMPPFFAAKLKANGGDPANSILARFGGSV
                     TVGGVRIATVTALHSNGVDPDYIGGDLGKAMKEAGIAGDVGLATGYVLKFSNGLVAYL
                     SGDTGIVADQDLVVRGHYGAKLAVMNIGDGFTTGPAEAAYVINDLVKPASVIASHANE
                     VGTVKGKVRPGSKTEAFMKAVKVPVHIPLSGKTMEFDNAGKCTAGC"
     gene            complement(2376..3233)
                     /gene="phhA"
                     /locus_tag="VFV55_00025"
     CDS             complement(2376..3233)
                     /gene="phhA"
                     /locus_tag="VFV55_00025"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX4942706.1"
                     /translation="MSGETTHGLRGDYAHMRADYTVEQDWPAYAPEEHALWRRLHARQ
                     AALVPRYACREFIESLAALDFGDAIPRFDVVNAKLAAATRWKLVAVPGLLPDTVFFMH
                     LAKRCFPVTVWLRTPREFDYIAEPDVFHDFFGHVPLLFDPVFADYLEAYGKGGLKAQA
                     LGAIEMLARLYWYTVEFGLVDTPAGIRAYGAGILSSGGELPCCIESGEPNRIGFDLLR
                     IMRTRYRIDAYQETYFVIRDFRELFEATAPDFTPYYAQLSGLAPHAPGAVLDGDRVVH
                     RGQASARTA"
     gene            complement(3238..>4212)
                     /locus_tag="VFV55_00030"
     CDS             complement(3238..>4212)
                     /locus_tag="VFV55_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4942707.1"
                     /translation="PTRDGTLVLVNRDLSTAVAASGISPTMQHALENWEAVYRTLLDA
                     YESLECGNERHAFDFQAALAQGRVAAPLPRAYEWLDGSAYLSHVERVRRARGDQVPES
                     FHREPLMYQGGAGDMMGPRDPVRALSEDWGIDLEGEVVAITGDVPMGAAPAEAAGAIR
                     LLTLVNDVSFRRLIPAELAKGFGFVNGKGANALAPVAVTPDELGSAWDAGKVHLRLVC
                     HVNGKWLGNPDAGVGATFDLHQLIAHAAKTRELRAGSMVGTGTISNPGRESGYACIME
                     ARLVEQVELGAAKMPFLAFGDTVRIEMFDHHGATIFGAIEQRVEKYAR"
BASE COUNT          737 a         1436 c         1409 g          630 t
ORIGIN      
        1 gatcgcgggc ctcgggctgg gcgctctgct gggctcgctc ttcagcggcg gcctgggcgg
       61 gatgggcggc atactcctgc tcctcctcgc cgccggcgtc gccttcatgg cgtaccgggc
      121 attcgcctcg cgccgggctc cggtcacgaa tccgccgatg cagttcgagg gtatcggcag
      181 cgacgtgccc gcgcgcccac ccctgaacat cggcggcgga atcaattccg caccggtcga
      241 gtcgccgcaa tcccaggcag cggcggcagc cattccgggc ttcgaagcgg agccgttcct
      301 gcgcgtggcg aagacctcct tcatccggtt gcaggcggcc aacgacgccc gggacctgga
      361 cgatatccgc gactacacca cgcccgagat gtacgcggag atctcgatgc agatccgcga
      421 acgcggcgat gcgatccaga aaacggaagt cgtgacgctg aatgcgagcc tcgtcgaggc
      481 cgtggtcgag ggcgactacg ccatcgccag cgtgcgcttc accgggctga tccgcgaagc
      541 ggccgacgcc aatcccgagc cgttcgacga gatctggcac gtgcgcaaga gcctcgtcga
      601 ccgcaaggcc acctggctca tcgcggggat ccagcaggca agttgagtcc cgttgcccgc
      661 atcagcgctt ggccggcgcg ggcagcagct ccacggtgat gggcttgcgc tcaacaggcg
      721 ggccgggcct cggcgtggag agatgcagga tggtccagaa tccgacgaaa gtggcgaatg
      781 ccagcgccgc aatgatcgcg atgcgagcga tccgggaccg gggcgacttg tcgggccggt
      841 aggcgccgac cgccttgtcg agcgcctttt cgatgaaatg gtcgcgaaag tccttcaccc
      901 gatgatcata aaccgcaggg tctccccgtg aatgccgaca cacccaagtc cacccgcaag
      961 gcacagctcg acgccctgct cctcaagctg ccgggcgtca gcacgaagaa gatcaacggc
     1021 ctcgacgcct acttcgtgtc cgaccggatg ttcgcctgca tcagcggcaa cggcatcggg
     1081 ctgcgcctgc ccgtggccgc cgcgaccgaa ctgcagttct cgcgggagaa cgtcgtcccg
     1141 ttccagcccg gcggcatggc gagcacgcgg gaatggatcc agatcgaccg cgccgacgcg
     1201 gccgactacg ggaaggatct cgagctgttc caggcctcga tcgaattcgt gaaaggcggc
     1261 cggacacgct aggccggcgg attcaccgtt atcatccgcc ggcaggactt tcgatcacaa
     1321 gaaacgataa ccggaggatt tcatgggaat gaacaatcgc tccacccgga tggctttcct
     1381 ggccgccatc ggcacgctgt cggcattttc cgcgctggcc cagacggtaa aggtgacgcc
     1441 gctcggcggc atcgacagcg agttctgccc gcaggatcgt gcgctggtct tcgaggaccc
     1501 gaacggtacg cgcatcctct acgatcccgg ccgcaccgtc gcgggcgcgg acgatccgcg
     1561 gctcggcaag atcgacatca tcctcgtgag ccacatgcac ggcgaccacg tgggcaacgc
     1621 gcacaacaag gcgccgaact cgggcagctg cgacaagccg gacgtatcgg tgtcggcgat
     1681 gccaaactcc agcgcggtga acattgcact cgccaagaag gcgaagatcg tgaccggcag
     1741 cgaaatgccg cccttcttcg cggcgaagct gaaagccaac ggtggcgatc cggcgaactc
     1801 gatcctggcc cgcttcggag gcagcgtgac cgtgggcggc gtgcgcatcg cgacggtcac
     1861 cgcgctgcac agcaatggcg tcgatcccga ctacatcggc ggtgacctgg gcaaggccat
     1921 gaaggaggcg ggcatcgccg gcgacgtggg cctcgcaacg ggctacgtcc tcaagttcag
     1981 caacgggctg gtggcctatc tctcgggcga taccggcatc gtggccgacc aggacctggt
     2041 cgtgcgcggc cactacggcg cgaagctcgc cgtcatgaac atcggcgacg gcttcaccac
     2101 cgggccggca gaggcggcct acgtcatcaa cgacctcgtg aaacccgcat ccgtcatcgc
     2161 ctcgcacgcg aacgaggtcg gcacggtgaa ggggaaggtg cgcccgggca gcaagacgga
     2221 agcgttcatg aaggccgtga aggtgcccgt gcacattccc ctttccggca agacgatgga
     2281 gttcgacaac gcgggcaagt gcacggccgg ttgctgatac gcccccgggc cgggtcgaac
     2341 ctgctggcgg gccgggcacg cggatccggc cctcctcacg cggtgcgcgc cgaagcctgc
     2401 ccgcggtgca ccacccggtc cccgtcgagc actgctccgg gcgcgtgcgg cgccaggccc
     2461 gagagttgcg cgtagtacgg cgtgaagtcc ggcgccgtcg cctcgaacag ctcccggaaa
     2521 tcgcggatca cgaaataggt ttcctggtag gcgtcgatcc tgtagcgcgt gcgcatgatg
     2581 cgaaggaggt cgaacccgat acgattgggc tccccgcttt cgatgcagca cggaagctcc
     2641 ccgcccgagg aaaggattcc ggccccatag gcacgaatgc ccgccggcgt gtcgacgagg
     2701 ccgaactcga ccgtgtacca gtaaaggcgc gcgagcatct cgatggcgcc cagggcctgc
     2761 gccttgagac cgcccttgcc gtaggcctcg aggtagtcgg cgaagacggg gtcgaaaagc
     2821 agcggcacgt gcccgaagaa atcgtggaag acgtcgggct cggcgatgta gtcgaattcc
     2881 cgcggcgtgc gcagccacac ggtgaccggg aaacaccgtt tggccaggtg catgaagaac
     2941 accgtgtcgg gcaggagtcc cggcaccgcg acgagtttcc aacgcgtggc cgcggcgagc
     3001 ttcgcgttca cgacgtcgaa acgcgggatg gcgtcgccga aatccagcgc ggccagcgat
     3061 tcgatgaact cgcggcaggc atagcgcgga accagtgccg cctggcgcgc atgcagtcgc
     3121 cgccacagcg cgtgctcctc cggcgcatag gcaggccaat cctgctcgac ggtgtagtcg
     3181 gcacgcatgt gagcgtagtc gccgcgcaac ccgtgggtcg tctcgccgga cacggcgcta
     3241 cctcgcgtac ttctccacgc gctgctcgat ggcgccgaag atcgtggcgc catggtggtc
     3301 gaacatctcg atgcgcacgg tatcgccgaa cgcgaggaac ggcatcttcg ccgcccccag
     3361 ctccacctgc tcgacgagcc ttgcctccat gatgcaggcg tagcccgatt cgcggcccgg
     3421 gttggagatc gtcccggttc cgaccatcga tcccgcccgc aactcgcgcg tcttcgccgc
     3481 atgcgcgatc aactggtgca ggtcgaacgt cgctcccact cccgcgtcgg gattgccgag
     3541 ccacttgccg ttcacgtggc agacgaggcg caggtggacc ttgccggcat cccaggcgct
     3601 gcccagctcg tccggcgtga ccgccaccgg ggccagggca ttggcgccct tgccgttcac
     3661 gaacccgaag cccttggcga gctccgcggg aatgaggcgc cggaacgaga cgtcgttcac
     3721 cagcgtgagc agccggatgg cgccggcagc ttccgcgggc gcggccccca tcggaacgtc
     3781 gccggtgatg gcgacgactt cgccctcgag gtcgatgccc cagtcctccg agagcgcgcg
     3841 aacgggatcg cgcgggccca tcatgtcgcc ggccccgccc tggtacatga ggggctcgcg
     3901 gtggaagctc tccggcacct ggtcgccgcg cgcccgccgc acgcgctcca cgtgcgaaag
     3961 gtaggccgat ccgtcgagcc actcgtaggc gcgcggcagc ggcgccgcca ccctgccctg
     4021 cgcaagcgcc gcctggaagt cgaacgcatg ccgctcgttg ccgcactcca ggctctcgta
     4081 cgcgtcgagg agcgtgcgat agaccgcttc ccagttctcc agcgcatgct gcatcgtggg
     4141 cgagatgccg gacgcggcga ccgcggtgga aaggtcgcga ttgacgagca cgagcgtgcc
     4201 gtcgcgggtg gg
//
LOCUS       DASUEV010000002         6028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_916763, whole genome shotgun sequence.
ACCESSION   DASUEV010000002 DASUEV010000000
VERSION     DASUEV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916763"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFV55_00035"
     CDS             complement(<1..375)
                     /locus_tag="VFV55_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX4942708.1"
                     /translation="MPDNYSISLSFLTSLAVGLLIGLERERHKDAKAGVRTFALIAML
                     GTICAMLGEKTGSPWILVAGLAASTYGVLSAYQIDSRSAPDAGTTTVMAAALTFCLGA
                     AVWYGYSGIVIAVAIVVTGLLHY"
     gene            517..768
                     /locus_tag="VFV55_00040"
     CDS             517..768
                     /locus_tag="VFV55_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942709.1"
                     /translation="MNTTVAIALLATAGALTGCASSDPKAGVPAAAPHASAAASAEEF
                     KTGSRLQRRAPESQPVKSIGREEWKNVVADKPQPMPAGN"
     gene            complement(904..1197)
                     /locus_tag="VFV55_00045"
     CDS             complement(904..1197)
                     /locus_tag="VFV55_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX4942710.1"
                     /translation="MRKTASHRTSGSDLTRVDAHRITPAEYKELPELTDDMLARAVVK
                     KGGRPRSVNPRQLISLRLPADVIARWRATGRGWQTRMAERLARTPVAKARNVA"
     gene            complement(1160..1435)
                     /locus_tag="VFV55_00050"
     CDS             complement(1160..1435)
                     /locus_tag="VFV55_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX4942711.1"
                     /translation="MRISYDPAKRLRTPRERGLDFEDADLVIKGVTVEVEDLRKDYGE
                     RRIICYGSLQGRQVVIGYTPRGAVRHIFSMRKANDREKDRLAPYFGL"
     gene            complement(1565..1640)
                     /locus_tag="VFV55_00055"
     tRNA            complement(1565..1640)
                     /locus_tag="VFV55_00055"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1605..1607),aa:Ala,seq:cgc)
     gene            complement(1676..2692)
                     /locus_tag="VFV55_00060"
     CDS             complement(1676..2692)
                     /locus_tag="VFV55_00060"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX4942712.1"
                     /translation="MRFCIVGAGAIGGFVGAKLALAGEEVTYIARGKNLEAINAEGMK
                     VFYRDGREEIASSVKATDDYAKAGTFDVVILALKAHQVGAVAEKLSPLFDANTAVIPM
                     QNGIPFWYFHDHGGELAGRTVETVDPGGVVKNAIPKERIIGCVVYPATELASPGKVIH
                     IEGDRFPIGELNGVATERATKISEAFTKAGLKCPLLDNVRAEMWLKLWGNLTFNPISA
                     LTHATLQDICEHPHGYALAAQMMKEAQSVAEKLGITFRVPLEKRIEGARKVGKHKTSM
                     LQDVEAGRPIEIDALVGSVVELGELAQVPVPTIKAVFLAAKLLDSTYTSQAVSIRAVQ
                     TDGS"
     gene            complement(2807..3619)
                     /gene="lgt"
                     /locus_tag="VFV55_00065"
     CDS             complement(2807..3619)
                     /gene="lgt"
                     /locus_tag="VFV55_00065"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX4942713.1"
                     /translation="MLTHPQFDPVFFSLGPVAVRWYGLMYVVGFVAYVMLGKLRVRDG
                     LGNGMTEEGVDDLLLYAVLGVILGGRLGYVIFYKPEYYLAHPLEIVQVWTGGMSFHGG
                     FLGVLLAVAIFCARRNLRWITTMDFIAPLVPLGLAAGRLGNFINGELPGRRTDLPWGM
                     WFPQVDHNPIARHPSQLYQFALEGLALFAILWWFSSKPRPAGAVSGAFLVGYGAFRFL
                     AEFTRQPDDFLGLLSLGLSMGQWLSLPMILAGGAMMAWAYRNRGIEGVDTRA"
     gene            3730..5403
                     /gene="ilvD"
                     /locus_tag="VFV55_00070"
     CDS             3730..5403
                     /gene="ilvD"
                     /locus_tag="VFV55_00070"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX4942714.1"
                     /translation="MTAKYRSTAITEGDARSPNRSMLRAIGYQDDDFRKPFIGLANGH
                     STLNPCNAGLQPLADAADAALRKAGGMPQMFGTITVTDGISMGTEGMKYSLVSREVIA
                     DSIETAVQSQFMDGVLAIGGCDKNMPGAMIALCRMNLPAIFVYGGTIKPGHYKGQDLQ
                     IVSVFEAVGSLNAGKMSREDFDCIERCALPGTGACGGMYTANTMSTAFEALGMSLPYS
                     STMANEDAEKVTSTEKSARVLVEAVKKGLRTRDIVTRKSLENAVAVVMAVGGSTNAVL
                     HLLAIAHAADIDWTIDDFERMRRKVPVLCDLKPSGRYVATDLHKAGGVPQVMKMLLGH
                     GLLHGDCVTISGKTIAEVLKDVPAEPSKDQDVIRPWSNPMYAQGHLAILRGNLAPEGC
                     VAKITGLKNPVITGPARVFDSEPDAMDAIMGDRIKAGDVLVIRYEGPRGGPGMREMLS
                     PTAALIGKGLGESVGLITDGRFSGGTWGMVVGHVAPEACEGGTIGLVKEGDSITIDAH
                     KLLIELNVPETEIAKRRAAWKKPEPRYTRGVLAKYAKLVGTASRGAVTD"
     gene            5537..>6028
                     /locus_tag="VFV55_00075"
     CDS             5537..>6028
                     /locus_tag="VFV55_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942715.1"
                     /translation="MSRILRLASFLVAVAAAWHSGAATVLPVSLDHLADNSAVIFEGT
                     CVGNRTERDEGTGLVVTYTTFSVRDVIKGDAASVHEIKQVGGQMPGDGLKFRVDGVPT
                     FAVGESYVVFLAGVSKAGFSSPIGLAQGRFVVSHEEAGKTVSNGRDFREMTTGMPDAL
                     LPFA"
BASE COUNT         1085 a         1970 c         2011 g          962 t
ORIGIN      
        1 gtagtgcagc agccccgtga ccacgatggc cactgcaatc acgattcccg agtagccgta
       61 ccagaccgcg gcgcccaggc agaaggtgag cgctgccgcc atgaccgtcg tggtgccggc
      121 atcgggcgca gaccgggagt cgatctggta ggcggaaagc acgccgtacg tgcttgccgc
      181 gaggcccgcc acgaggatcc atggcgagcc ggtcttctcg cccagcatcg cgcagatcgt
      241 ccccagcatc gcgatcagcg cgaaggtgcg gacccccgcc ttcgcgtcct tgtggcgctc
      301 gcgctcgagg ccgatcagaa ggccgacggc aaggctcgtc aggaaggaaa gcgagatgga
      361 atagttgtcc ggcatggccc gagcgcaccc tggttcattc tgcggcaggc tgcgggccgg
      421 ggcttgactc gcgtcaacgc aagccaaaag ccgtggcatc atttgcactt cgtccacggg
      481 atcttccgtg gcagccacat cgatccgggg agccccatga ataccactgt cgccatcgcc
      541 ctgctcgcca ccgctggcgc cctcaccggc tgcgcatcca gcgacccgaa ggcgggcgtt
      601 cccgccgcgg ccccgcacgc aagcgcggcc gcgagtgccg aggagttcaa gacgggcagc
      661 cgcctgcagc gaagagcccc tgaatcgcag ccggtgaaat cgatcggccg cgaggaatgg
      721 aagaacgtgg tggcggacaa gccgcagccc atgccggctg ggaactgagc ccggaggcag
      781 ttgccgcgct cggacagcgc atctccgaac acggagaaca ggcggtttcg ctcggcgttg
      841 tgccggggac gaacgggggc ctacaggaag cgccgcctcg aatcagcaac tttccgcggc
      901 gggtcaggcc acgtttcttg ccttcgcaac cggtgtcctc gcaagccgtt ccgccattcg
      961 cgtttgccag ccgcgacctg ttgccctcca gcgcgcaatc acgtctgcag gcagtcgcag
     1021 ggagatgagt tgccgagggt tgaccgagcg aggacggccg cccttcttga cgacagcccg
     1081 ggcgagcatg tcgtccgtga gctcgggaag ctccttatac tccgcgggcg tgatccgatg
     1141 cgcgtccacg cgcgtgagat cagagcccga agtacggtgc gaggcggtct ttctcacggt
     1201 cgttggcctt tctcatgctg aagatgtggc gcaccgcgcc acgcggggtg tagccgatca
     1261 ctacctgccg gccctgaaga cttccgtagc agatgattct gcgctcaccg tagtccttgc
     1321 gaagatcctc gacctcgacg gtaacgcctt tgattacaag gtcagcatcc tcgaaatcca
     1381 gtccacgttc cctcggggta cgtaagcgct ttgccgggtc gtagctgatt cgcacccgat
     1441 tattgtatct acaataacag cctcgcgcca gtagcgcgcc cgtcggcggt cgtgcgcgag
     1501 cctagtccac tatgggaaat tccgggcgaa aaaaaggcca gagcagtgtc tggccttgga
     1561 tcggtggtgg agtggaaggg gatcgaaccc tcgacctccg cattgcgaac gcggcgctct
     1621 cccagctgag ctaccacccc atcgagaagc gcgaaattat agcggatttc cggcttcagc
     1681 tcccgtcggt ctgcacggca cggatgctga ccgcctgaga agtgtaggtg ctgtcaagaa
     1741 gcttcgcggc caggaagacc gccttgatgg tgggaacggg cacctgggcc agctctccca
     1801 gctccaccac ggagcccacc agggcgtcga tctcgatggg acggccggcc tccacgtcct
     1861 gcagcatcga ggtcttgtgc ttgcccacct tgcgcgcgcc ctcgatgcgc ttctcgagcg
     1921 gtacgcggaa ggtgatgccc agtttctccg ccaccgactg cgcttccttc atcatctgcg
     1981 cggcgagcgc gtaaccgtgc ggatgctcgc agatgtcctg caacgtcgcg tgggtgagcg
     2041 cggagatcgg gttgaaggtg aggttgcccc agagtttcag ccacatctcg gcgcgcacgt
     2101 tgtcgaggag agggcacttg agcccggcct tcgtgaacgc ttccgaaatc ttcgtggcac
     2161 gctcggtcgc cacgccgttc agctccccga tgggaaagcg atcaccctcg atgtggatga
     2221 ccttgccggg agaggcgagc tcggtggcgg gatagaccac gcagccgatg atgcgctcct
     2281 tcgggatggc gttcttcacc accccgcccg ggtccaccgt ctcgacggtg cgcccggcaa
     2341 gctcgccgcc gtggtcgtgg aaataccaga acgggatccc gttctgcatc gggatcaccg
     2401 ccgtgttcgc gtcgaagagg ggggagagct tttccgccac cgcgcccacc tggtgcgcct
     2461 tgagcgcgag gatcaccacg tcgaaggtgc ccgccttcgc gtagtcgtcc gtggccttca
     2521 cggaggaggc gatctcctcg cgcccgtcgc ggtagaagac cttcattccc tcggcgttga
     2581 tggcctcgag gttcttgccg cgggcgatgt aggtgacttc ctcgccggcc agggccagct
     2641 tcgcgccgac gaagcccccg atggcaccgg cgccaacgat gcagaaacgc atttttcccc
     2701 cagtggacga actgccatcc ccgccgcgcg ggcggggcgg gtagcccgtg gccgcggcag
     2761 gacttcaacc gccaaggttg caacttatgg tgcagcgcac aaactgtcaa gcgcgggtat
     2821 caacgccttc tatcccgcgg tttcgatacg cccacgccat catcgcgccc cccgccagga
     2881 tcatcggcag cgacagccat tgccccatgg aaaggccgag cgaaagcagg ccgaggaaat
     2941 cgtcgggctg gcgggtgaat tccgcgagga agcggaatgc gccatacccc acgaggaatg
     3001 cgcccgagac ggcgccggcg gggcggggct tcgacgagaa ccaccagagg atggcgaaga
     3061 gcgccaggcc ctccagcgcg aactggtaga gctgcgaagg gtgacgggcg atcggattgt
     3121 ggtccacctg cgggaaccac atgccccagg gcaggtccgt gcgccgcccc ggcagctcgc
     3181 cgttgatgaa gttgcccagg cgccccgccg cgaggccgag gggcaccagg ggcgcgatga
     3241 agtccatggt ggtgatccag cgaagattgc gccgcgcgca gaagatcgca accgccagga
     3301 gcacgccgag gaagccgccg tggaagctca ttcccccggt ccacacctgg acgatctcca
     3361 gcgggtgcgc gaggtaatac tccggcttgt agaaaatcac atagcccagc cggcctccca
     3421 ggatcacgcc gagcacggcg tagagaagca ggtcgtccac gccctcctcc gtcatcccgt
     3481 tgcccagccc gtcgcgcacg cgcagcttgc ccagcatcac gtaggccacg aagcccacga
     3541 cgtacatgag gccataccag cgcacggcga cggggccgag gctgaagaag acggggtcga
     3601 actgaggatg cgtcaacacg ggtatggatg ccttgtgagg gtttcgcggg gtgcccgcga
     3661 ccggctattc taccaatccg gccccgccaa tctcggccgc ggcccgcaat tccccgggaa
     3721 aaccccacca tgaccgccaa gtaccgctcc accgccatca ccgaaggcga cgcccgctcg
     3781 cccaaccgtt ccatgctgcg cgccatcggc taccaggacg acgattttag gaagcccttc
     3841 atcgggctcg ccaacgggca ctcgacgctc aacccctgca acgctgggct gcagccgctt
     3901 gccgacgccg ccgacgcggc gctgcgcaag gcgggcggca tgccgcagat gttcggcacc
     3961 atcaccgtga ccgacggcat ctccatgggc acggagggca tgaagtactc cctcgtctcg
     4021 cgcgaggtga tcgccgactc catcgagaca gcggtccaga gccagttcat ggacggggtg
     4081 ctcgccatcg gcggctgcga caagaacatg cccggcgcga tgatcgccct gtgccggatg
     4141 aacctgcctg cgatcttcgt gtacggcggc acgatcaagc ccggccacta caagggccag
     4201 gacctgcaga tcgtctcggt gttcgaggcg gtgggatcgc tcaacgccgg caagatgagc
     4261 cgcgaggatt tcgactgcat cgagcgctgc gcgctgcccg gcaccggcgc gtgcggcggc
     4321 atgtacaccg cgaacaccat gtccaccgcg ttcgaggcgc tgggcatgtc gctgccctac
     4381 tcgtcgacga tggccaacga ggatgcggag aaggtcacga gcacggagaa gagcgcgcgc
     4441 gtgctggtgg aggcggtgaa gaagggtctc aggactcgcg acatcgtcac gcgcaagtcc
     4501 ctcgagaacg cggtcgcggt cgtcatggcg gtgggcggct ccaccaacgc ggtgctgcac
     4561 ctgctcgcga tcgcgcacgc ggcggacatc gactggacca ttgacgactt cgagcgcatg
     4621 cgccgcaagg tgcccgttct ctgcgacctg aagcccagcg ggcgctacgt ggccaccgac
     4681 ctgcacaagg ccggcggcgt gccccaggtg atgaagatgc tgctcgggca cgggctgctg
     4741 cacggcgact gcgtgaccat ctcgggcaag accatcgccg aggtgctgaa ggacgttccc
     4801 gccgagccga gcaaggacca ggacgtgatc cgcccgtggt cgaacccgat gtacgcgcag
     4861 ggccacctcg ccatcctcag gggcaacctc gcgcccgagg ggtgcgtcgc caagatcacg
     4921 ggcctgaaga acccggtcat tacggggccc gcgcgcgtgt tcgattccga gcccgatgcg
     4981 atggacgcga tcatgggtga ccgcatcaag gcgggcgacg tgctcgtcat ccgctacgag
     5041 ggccccaggg gcggccccgg catgcgcgag atgctctcgc ctacggcggc gttgatcgga
     5101 aagggattgg gggagtcagt cggtctcatc accgacgggc gattctcggg cggcacctgg
     5161 ggcatggtcg tggggcacgt ggcgcccgag gcgtgcgaag gcggcacgat cgggctggtg
     5221 aaggaaggcg actcgatcac catcgacgcg cacaaactcc tcatcgagtt gaacgtgccg
     5281 gaaacggaga tcgcgaaacg acgtgctgcc tggaagaagc ccgagccgcg ctatacgcgc
     5341 ggggtgctgg ccaagtacgc gaagctggtg gggacggcga gcaggggggc ggtgacggac
     5401 tgaaggtagc agcccgcggg gcacggagat gccacggaag ttcgtcgtcg cgccgcaccc
     5461 gcgtgaagcg taacccggca gtgcttgatg cggtgcacaa tgcagatgtc aagtctgcga
     5521 atctgaattg aagcaaatgt cgaggatcct gaggctcgcg tcttttctgg tggcggtcgc
     5581 ggcggcgtgg cattcgggtg cggcgacggt gctccccgtc tcccttgacc acctggcgga
     5641 caactctgcc gtgatcttcg agggcacatg cgtcggcaac cgcacggagc gcgacgaggg
     5701 cacgggcctg gtggtgacgt acacgacctt ttccgtccgc gacgtgatca agggtgacgc
     5761 ggcgtccgtc cacgaaatca agcaggtggg cgggcagatg cccggggacg ggctgaagtt
     5821 ccgcgtggac ggcgtgccga cctttgcggt cggagagtcg tacgtggtgt tcctcgcggg
     5881 cgtctcgaag gccggctttt cgagcccgat cggcctcgcg cagggccggt tcgtcgtttc
     5941 ccacgaagaa gccgggaaga cggtctccaa cggccgcgat ttccgcgaaa tgacgaccgg
     6001 catgccggac gccctgctgc ctttcgcg
//
LOCUS       DASUEV010000003         6058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1145697, whole genome shotgun sequence.
ACCESSION   DASUEV010000003 DASUEV010000000
VERSION     DASUEV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145697"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VFV55_00080"
     CDS             <1..1274
                     /locus_tag="VFV55_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4942716.1"
                     /translation="AVAAAGDWLGPVKGPVANFILRHVKKAVRPWAIEGAVRFDELLA
                     QGAKLPFAAPTIRAEDVALLQYTGGTTGVSKGAMLTHANVVAAVLAADAWLAPAFAGE
                     RRIDNPTMVCALPLYHIYALVACLFLGLRLGARNLLIANPRDLDALVRDLRRNPPHLF
                     PAVNTLYNALLDHPGFGDIDFSGLRVANSGGMAVQRAVADRWLARTGCPIVEGWGLTE
                     STAGVTCNRVDVREFSGTVGLPFPGCEIDVRDVLGRPLPPGEAGEVVVRGPQVMAGYW
                     RKPEETARAFTGDGWLRTGDIGVMDANGELRIVDRMKDMIIVSGFNVYPNEIEDVATL
                     HPGVREAAAIGIPDDKCGEAVKLFVVRRDAALAEAQLAAFLRERLTGYKCPRHYAFVE
                     ALPKTPVGKILRRALREKGAAWRPGTPDIQR"
     gene            complement(1265..2059)
                     /locus_tag="VFV55_00085"
     CDS             complement(1265..2059)
                     /locus_tag="VFV55_00085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4942717.1"
                     /translation="MNPKTPPAAAPDSPIAALPKYWADEHARRRFVDDLFDHSAHDYD
                     RFERILALGTGSRYRRDALRRSGLRPGMRVLDIATGTGLVAREALALVGEQGGVIGLD
                     PSAGMLVEAHCALPIPLVRAFGERIPCRDASFDFVSMGFALRHVADLGLLFREIRRVL
                     RPGGTACILEITRPASPLAAEAVRVFMTRVMPVFAGRASRRAEARKLMNFYWDTINAC
                     VRPEAVLAALAGAGFDAPRRAVVLRLFSEYVATAPSALAPASDIHR"
     gene            2234..3187
                     /locus_tag="VFV55_00090"
     CDS             2234..3187
                     /locus_tag="VFV55_00090"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942718.1"
                     /translation="MGFPIVPAACAADVPSACVTAASLRPGARSSGLCEVWVSEDPVR
                     RHVDGAIRYAISGDYLFGVIAHAHGKGALRDLAERAYGEIFAMLGRHGFPHVVRFWNY
                     IPRINEEDGGVERYRLFNIGRGAAFESAGRSRAETIPAACALGTDDSGSALTVYFVAA
                     RQRGRAVENPRQVSAYRYPPEHGPHSPTFSRAVLHPADDARMLFVSGTASIVGHLTLH
                     VGEVVAQTRETLANLEAVFAEACRLSRPGAFTMREASYKVYVRHAADVEAVRRELAAA
                     LGEDADVNFLRADICRSDLLVEIEAIAYAGDPGGGTSAGIP"
     gene            3199..4068
                     /locus_tag="VFV55_00095"
     CDS             3199..4068
                     /locus_tag="VFV55_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX4942719.1"
                     /translation="MRKAPRSGVATFFVAMPVLASAAVLASGSAFAQGHEEPLWEAGA
                     GVAALRLPDYRGASHSRWYALPLPYLVYRGDFLKADRHGVRGVFFDSDRVEFNLSVGA
                     SLPVDSSQSEARQGMPDLRPSVELGPSVDFTLWRSAARRAKVELRLPLRGAVTIESDP
                     RYIGVQFFPHLSLDVNDVGGLAGWNLGMLAGPVYTDRRYNRYFYGVPPDQATAARPAY
                     DPDGGYGGAQFIVALSRRFARLWVGGYLRQDTLAGATFAASPLVTSRRYMAGGIGVSW
                     IFSESSRRVPAND"
     gene            4094..4912
                     /locus_tag="VFV55_00100"
     CDS             4094..4912
                     /locus_tag="VFV55_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4942720.1"
                     /translation="MPILRVLGAIRDYAILAFGITLLALMCAAWGVVSAVFLLVLPAR
                     TGRRVGRVVAMFGFRSYLAIMQALGALRLDLAELDNLREEPPLIVAPNHPSLIDAVLV
                     VSRLPDAMCVMKASLLGNFLLGPAARLARYIRNDTLLGLAARAGEELRHGGHLVLFPE
                     GTRTAREPVGPFTGAAAVIARRTGVPVQAVFIEADSRFLGKGWPVHARPAFPLRYRAR
                     LGRRFDPPKDVRAFTAELESYFAQELGGRPAQAGFGAPTRSFAGVPPDVRDLHD"
     gene            4905..5663
                     /locus_tag="VFV55_00105"
     CDS             4905..5663
                     /locus_tag="VFV55_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4942721.1"
                     /translation="MTEHSATHLVLIPSYNPGPAGYETVRAARRNWDPVWVIVDGSND
                     GTAEGFAEMARSDAGLRVVVLPANGGKGAAVLHGITLAAAEGYTHALTMDSDGQHPAT
                     SIPEFMAASQAMPRVVVLGVPVFDAAAPSLRVKGRRVSNWWANLETLWLGIGDSLFGF
                     RVYPIADLVAIMRRQPWMRRFDFDPEAAVRLVWRGVPTVNVPAPVRYLRPEEGGVSHF
                     NYVRDNALLTWMHTRLVLEFLVRLPALVLRRLRA"
BASE COUNT          857 a         2071 c         2168 g          962 t
ORIGIN      
        1 tggcggtcgc cgcggcgggc gactggctgg gtccggtgaa ggggccggtc gcgaacttca
       61 tcctgcgtca cgtgaagaag gcggtgcggc cctgggccat cgagggcgcg gtgcgcttcg
      121 acgaactgct cgcgcagggt gcgaagcttc ctttcgcagc tcccacgatc cgcgcggagg
      181 atgtggcgct cctgcaatac accggcggca cgacgggagt atcgaagggc gcgatgctca
      241 cgcacgccaa cgtggtggct gcggtgctcg cggccgatgc ctggctcgcg ccagccttcg
      301 cgggcgagcg ccgcatcgac aacccgacga tggtgtgtgc cctgccgctg tatcacatct
      361 atgcgctggt ggcgtgcctc ttcctcggcc tgcgcctggg tgcgcggaac ctgctcattg
      421 ccaacccgcg cgacctcgac gccctcgtgc gcgacctgcg ccgcaacccc ccgcacctct
      481 ttccggcggt gaacacgctc tacaacgcgc tcctcgatca cccgggcttc ggcgacatcg
      541 acttttccgg cctgagggtg gccaacagcg gcggcatggc ggtgcagcgc gccgtggccg
      601 accgctggct cgcgcgcacc ggctgcccca tcgtcgaggg atgggggctc accgagtcga
      661 cggcgggcgt cacctgcaac cgggtggacg ttcgcgaatt cagcggcacg gtgggacttc
      721 cattcccggg ttgcgagatt gacgtgcgcg acgtgctggg ccggccgctg cccccgggag
      781 aagcgggcga ggtcgtggtg cgcgggccgc aggtcatggc cggctactgg cgcaagccag
      841 aggagacggc gcgcgccttc accggcgacg gctggcttcg caccggcgac atcggcgtga
      901 tggacgcgaa tggcgagctg cgcatcgtcg atcgcatgaa ggacatgatc atcgtgtcgg
      961 gattcaacgt gtacccgaac gagatcgagg acgtggcgac gctgcatccg ggcgtgcgcg
     1021 aggcggccgc catcggcatt cccgacgata agtgcggcga ggcggtgaag ctcttcgtgg
     1081 tgcggcgcga cgctgcgctg gcggaagcgc agctggctgc gttcctgcgc gagcggctca
     1141 ccggctacaa gtgcccgcgg cactacgctt tcgtggaggc gctgcccaag acgcccgtgg
     1201 gcaagatcct gcggcgagcg ttgcgcgaga agggcgcggc ctggcggccg ggaacacccg
     1261 acattcagcg gtgaatgtcg cttgccggcg ccagcgcgga gggcgccgtg gcgacgtatt
     1321 cggaaaagag ccgcaggacc acggcacgac gcggcgcgtc gaacccggcg ccagcgagcg
     1381 cggccagcac ggcttcgggc cgcacgcacg cgttgatggt gtcccaatag aagttcatca
     1441 gcttgcgggc ctccgcgcgc cgggaagcgc ggcccgcgaa gaccggcatg acgcgggtca
     1501 tgaaaacgcg caccgcctcc gcggcgagcg gcgaagcggg acgcgtgatc tcgagtatgc
     1561 atgccgtgcc gccggggcga agcacgcggc gaatctcgcg gaagagcagc cccaggtcgg
     1621 ccacgtggcg cagggcgaaa cccatgctca cgaagtcgaa gctcgcgtcg cggcagggga
     1681 tgcgctcgcc gaaggctcga accagcggaa tgggaagggc gcaatgagcc tcgacgagca
     1741 tgcccgcgct gggatccagg ccgatcaccc cgccctgctc gccgacgagg gcgagggctt
     1801 cgcgcgccac gagccccgtt cccgtggcga tgtccaggac gcgcatgccc gggcgcaatc
     1861 cggagcggcg caacgcgtcg cggcgatagc gcgagccggt gccgagcgcg aggatgcgct
     1921 cgaagcggtc gtagtcgtgg gcgctgtggt cgaagaggtc gtccacgaat cgccggcggg
     1981 catgctcgtc ggcccagtac ttcggaaggg ccgcgatcgg cgaatcggga gcggcggcgg
     2041 gaggcgtctt cggattcatc gcgacacggt ccatcgcgga aggaaaggca atatacaata
     2101 tctttcggta cctgccggcc gcaccgggcg gcggcaattt caggagcgcc acgacatact
     2161 gcacatcgaa tacctcccgc tcgatgcagc cacggacttc atcgggcgtg cgccccccgg
     2221 gctcttcggg gtgatggggt tcccgatagt gcccgcggcg tgcgcggccg acgtgccatc
     2281 cgcgtgcgtg actgccgcga gcctgcgccc cggcgcccgc tcgtccggcc tttgcgaggt
     2341 gtgggtatcg gaggacccgg ttcggcggca cgtcgacggg gccatccggt acgcgatctc
     2401 gggcgattat cttttcggcg tcatcgcgca tgctcacggg aaaggcgcgc ttcgcgacct
     2461 cgccgagcgt gcctacggcg aaatcttcgc catgctcggc aggcacgggt tcccccatgt
     2521 cgtgcgcttc tggaactaca ttccgcgcat caacgaggaa gacggcggcg tcgagcgcta
     2581 ccggcttttc aacatcggcc gcggcgcggc cttcgagtcg gccgggcgtt cccgggcgga
     2641 gacgattccc gccgcgtgcg cgctgggcac cgacgattcc ggcagcgcgc tcacggttta
     2701 cttcgtggca gcgcgccaac gcgggcgcgc cgtggaaaac ccgcggcagg tgagcgccta
     2761 tcgctatccg ccggagcatg gcccgcacag ccccaccttt tcgcgcgccg tgctgcatcc
     2821 ggcggacgac gcgcgcatgc tcttcgtctc cggcacggcg agcatcgtcg gccacctcac
     2881 gctgcacgtc ggcgaagtcg tggcacagac gcgcgagacg ctcgccaacc tcgaggccgt
     2941 cttcgccgag gcatgccgac tctcccggcc cggcgccttc acgatgcgcg aggcgagcta
     3001 caaggtctat gtccggcacg ccgccgacgt ggaagccgtc cggcgggaac tggcagcggc
     3061 gctgggagag gacgccgacg tcaacttcct gcgcgcggac atctgccgca gcgacctgct
     3121 ggtggagatc gaggcgatcg catacgccgg cgatcccggg ggcggcacgt ccgccggcat
     3181 cccatgaagg cgccgttcat gcgcaaggcc ccgaggtcgg gcgtggccac gttcttcgtc
     3241 gcgatgccgg tcctcgcctc ggcggccgtc cttgcctccg ggtcggcttt cgcccaggga
     3301 catgaggagc cgctatggga ggccggcgcg ggcgtggcgg cccttcgact tccggactat
     3361 cgcggcgcct cgcacagccg ctggtacgcg cttcccctgc cgtacctggt ctatcgcggc
     3421 gatttcctca aggccgaccg ccacggcgtg cgcggggtgt tcttcgattc cgaccgggtc
     3481 gagttcaacc tctccgtggg cgcgtcgctg cccgtggaca gctcgcaaag cgaggcgcgc
     3541 cagggcatgc ccgacctgcg gccctcggtg gaactggggc cgtccgtcga cttcacgctc
     3601 tggcgctcgg ccgcgcgccg cgccaaggtg gaactgcggc ttcccctgcg cggggcggtc
     3661 acgatcgaat ccgacccgcg ctacatcggc gtgcaattct tcccgcacct gagtctcgac
     3721 gtgaacgacg tgggcggcct ggccggctgg aacctgggca tgctggcggg gccggtgtac
     3781 acggaccgcc ggtacaaccg ctatttctat ggcgtgccgc cggaccaggc gaccgccgcc
     3841 cgccctgcct atgaccccga cggcggttat ggcggcgcgc agttcatcgt ggccttgtcc
     3901 aggcgtttcg cgaggctctg ggtaggcggc tacctgcggc aggacacgct cgcgggcgcc
     3961 acgttcgctg cgagcccgct ggtaacctcc cgccgttaca tggccggggg catcggcgtg
     4021 tcgtggatct tctccgagtc ttcccggcgc gtgccggcga acgactgaga gcgggggagc
     4081 cggggagccg gtcatgccga tcctgcgggt actgggcgcg atccgggatt acgcgatcct
     4141 ggcattcggg atcacgctgc tggccttgat gtgcgcggcc tggggcgtgg tgtccgccgt
     4201 gttcctcctg gtgctgcccg cgcgaacggg gcgccgcgtc gggcgcgtcg tcgcgatgtt
     4261 cggcttccgg tcctatctcg ccatcatgca ggcgctgggc gcgttgcgcc tggacctcgc
     4321 cgagctcgac aacctgcgcg aggagccgcc cctcatcgtc gcaccgaacc atccgtcgct
     4381 gatcgatgcg gtgctcgtcg tttcgcgcct gcccgacgcg atgtgcgtga tgaaggcctc
     4441 gctcctgggc aacttcctcc tcggtcccgc cgcgcggctg gctcgctaca tccgcaacga
     4501 cacgctcctc ggtctcgcgg cgcgcgcagg cgaggagttg cgccacggcg ggcacctcgt
     4561 gctcttcccc gagggcacgc gcaccgcgcg cgagccggtg gggcccttca cgggggcggc
     4621 ggcggtcatc gcgcgccgca cgggcgtgcc ggtgcaggcg gtcttcatcg aggccgactc
     4681 gcgattcctc ggcaagggct ggccggtgca cgcgcgcccg gcgtttccgc tccggtaccg
     4741 cgcgcggctc gggcgccgct tcgacccgcc gaaggatgtg cgcgccttca cggccgagct
     4801 ggagagctac tttgcgcagg agctgggcgg ccggccggcg caggcgggat tcggtgcgcc
     4861 cacgcgatcc ttcgcgggag tgcctccgga cgtgcgcgac ctgcatgact gagcattcgg
     4921 cgacgcacct cgtcctcatc cccagctaca acccggggcc ggccggctac gagacggtgc
     4981 gcgcggcacg gcgcaactgg gatccggtat gggtgatcgt ggacggcagc aacgacggga
     5041 ccgcggaggg cttcgcggag atggcccggt ccgacgccgg gcttcgcgtg gtcgtgctgc
     5101 cggccaacgg cggcaagggg gccgcggtgc tgcacggcat cacgctcgcg gcggccgagg
     5161 ggtacacgca cgcgctcacc atggactccg acgggcagca cccggccacg agcattcccg
     5221 agttcatggc ggcgtcgcag gcgatgcccc gggtggtcgt gctgggcgtg ccggtgttcg
     5281 acgcggcggc gccctcgttg cgcgtgaagg gccggcgcgt gtcgaactgg tgggcgaacc
     5341 tcgagacgct gtggctcggc atcggcgatt cgctcttcgg ctttcgcgtc tacccgattg
     5401 cggacctcgt ggccatcatg cggcgccagc cgtggatgcg gcgcttcgac ttcgacccgg
     5461 aagcggccgt gcgcctggtg tggcgcggcg tgccgacggt gaacgtgccc gcgcccgtgc
     5521 gttacctgcg gcccgaggag ggcggcgtgt cgcacttcaa ctacgtgcgc gacaacgcgc
     5581 tcctcacctg gatgcacacg cggctcgtcc tcgagttcct ggtgcggctg ccggcgctcg
     5641 tgctccggcg gctgcgcgcg tagagggcga cggtgcctgg agtgaggcga cggacccatc
     5701 gtggatcgcg gtgcccgatg cgaggcgacg gacccatcgc ggatagcggt aacggatgcc
     5761 aggcgccgga cccatttgcg gcagggctcc tgtgggtggg tgtttttttt ggtttttggg
     5821 ccgagtcaat atgcttctgg cgtcacgggt cgaacctatg tcgaagtgct catggcatcg
     5881 aaactgacgg caagtccgtt cggcccaaaa accaaaaaag aacacccacc cacaggagcc
     5941 cggtagtgcc catggtccgc agcgacctca ctccatgcat cgcttccaat ggtccacagc
     6001 gacgtcactc catgcatcgc ttccaatggt ccacagcgac gtcactccat gcatcgct
//
LOCUS       DASUEV010000004         5746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_573708, whole genome shotgun sequence.
ACCESSION   DASUEV010000004 DASUEV010000000
VERSION     DASUEV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573708"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..612)
                     /locus_tag="VFV55_00110"
     CDS             complement(211..612)
                     /locus_tag="VFV55_00110"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX4942722.1"
                     /translation="MSPFPRHQKETLLTTLGMEIVEATKERVVARMPVGPKVHQPFGL
                     LHGGASVALAETVASTAAWMNVDQDTEIVVGIEINANHLRAKRDGVVTATATALHVGR
                     RTQVWDVRIADEEGRTVCASRCTLAVVPKPK"
     gene            complement(627..2153)
                     /locus_tag="VFV55_00115"
     CDS             complement(627..2153)
                     /locus_tag="VFV55_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4942723.1"
                     /translation="MKVRLRSMRLSMHSPRHAIAALLFAAVAGCGGGGGGSPADATPL
                     PPTRSFPPSAGLAAHCAVPRPGTDPFHGNRPYPDLQGSLDDEKRWVRSWIDETYLWYR
                     EVPDPPAAAYAGPVEYFNVLRTPATTASGNPRDRFHFVFDTTAWNQLSLAGVEAGYGI
                     EWALLARSPPRRMVVANVDPGSPAANAGIVRGAEVVSVDGVDFVNGADAAAINAGAFP
                     DRAGKQTIFVFRDPGATAPRTVTLAAIAVTKVPVQDVRTVDTPAGRVGYLLFTDHIAT
                     AEGALAEAVGQLRDAGASELVLDMRYNGGGFLDIAAELAYMIAGPERTAGRVFERLRF
                     NDRNPFGYDPAGLVTPFHATTQGFSIAAGTALPTLSLARVTVLTSSGTCSASESVINA
                     LRGVDVEVNLVGGTTCGKPYGFLPQDNCGTTYFAIQFEGLNDRDFGDYADGFAPTCTV
                     ADDFTRPLGDPAEGRFAAALELLATGACPAGTATTAKSLLRDPELVRSPARENRILRR
                     "
     gene            complement(2150..4000)
                     /locus_tag="VFV55_00120"
     CDS             complement(2150..4000)
                     /locus_tag="VFV55_00120"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX4942724.1"
                     /translation="MFYRLVLAIAIAGLPATAIAQRTQKPPLHGQEWMAVTGKPLGAA
                     AGAKVFLKGGNAVDAACAMIAATATMWDTLHWGGETQALVWNPHTKKVIGINALGVAP
                     AGATPEFFRAKGLDYPPPYGPLAAVTPGTPGGILTMLAEFGKLSLAEVLEPAIAMADG
                     YPIEAQLAGYIERQRAHLMQWPDSRRIMLPGAADHAPEAGEMFRQPDLAATLRKLVEA
                     EKAALAAGKTRRQAIEAAQDRFYRGDIARDLVASVRAQGGLFTEADLAGWKVRLEEPV
                     MTSYKGIDVYKLTAWTQGPVMLQALNILGNFDLKAMGYNSTRYIHTVYQAMNLAYADR
                     DFYYGDTYTPPEEPVAGLLSKEYARQRAKLVDPMKNDPKVAPGDPYPFQRAANPYLDL
                     LKSWQEPRKKEPGKGGTPMAANDAAFERAFYSGTTSVIAADKEGWVVSVTPSGGWVPA
                     VIAGKTGIGLSQRMQSFVMDPAENPFNVVAPGKYPRVTLTPSLALKDGQPYLAFAVQG
                     GDGQDQDLLQFFLNVVEFGMTPQEAAEAPGFNSYQMRASFEAHESRPGRIMLNDATPP
                     YVRKELRAMGYDPIYRDRTSGPVNAIMIDRRHGTLWGASSNHGEDYGIGW"
     gene            complement(4028..>5746)
                     /locus_tag="VFV55_00125"
     CDS             complement(4028..>5746)
                     /locus_tag="VFV55_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX4942725.1"
                     /translation="APEPALHALFEREFLRGQEEFPEFATMIGNPAFNDRLHDGSPEA
                     VARRKAHVKAVLADLGRFDPKRLSTQDRISLEMMKDSLRRRDELNALFGDLPFGAGRG
                     DGWMQVSPSSGPQVILPMLARAAPFRDARDYENYLKRLEAVPRVLAESRVVMEAGIES
                     GWMPPRAAMSQVPGQLAVFSGGDATATPLYAPFRQFPAAVAAQDRARLAEAARITLAQ
                     RVQPAFAAFRRFVEEEYLPACRDDLAASTLPAGAAYYRMMIATMTTTDLDAAQIHEIG
                     KREVVRIRAEMDRVIASTGFKGSFAAFVQSIKTNPRSYHTSAHSMLEHYRDIAKRADA
                     ELPRFFAELPRLPYGIRPMPAYLGDNAEHYTPGALDGSRAGFFEANTNNLSRRARHDM
                     EAVLLHEAVPGHHLQTARAQELKGLPSFRRASFYVAYGEGWALYAESLGEEMGLYADP
                     LSKFGRLSMEMWRACRLVVDTGLHAFGWTREQSIRYLADTSGIDESAAIAEVDRYILD
                     PGQALGYKIGELRIKALRAKAQQALGERFDLRRFHNAVLDDGALPLNLLDARIDEWIA
                     REKAAR"
BASE COUNT          832 a         1948 c         2020 g          946 t
ORIGIN      
        1 aatgggtaaa aatccgaggg cagacgtgtt tggctgacct cggatttttg ggacccattc
       61 gacggtcacg tttgcgtgcc caggacgccg attaacttgc cccatccccg gctcgactcc
      121 ccttcgccgt tcgcgttctc aggacgccga tagacttgcc ccatcgccgg cccacctctc
      181 attcgccgca cacgcctgcg ggcccggtgg tcactttggc ttcggtacga cggcgagcgt
      241 gcagcgcgag gcgcacacgg tccggccctc ctcgtccgcg atgcgcacgt cccacacctg
      301 cgtgcgccgg cccacgtgca gcgccgtggc ggtagcggtc acgacgccgt ctcgcttggc
      361 ccgcaggtgg ttcgcgttga tctcgatgcc gaccacgatc tcggtgtcct ggtccacgtt
      421 catccacgcc gccgtcgagg ccaccgtttc ggccagcgcc accgaggcgc cgccgtgcag
      481 caggccgaac ggctggtgaa ccttcgggcc gacgggcatg cgggcgacca cgcgttcctt
      541 cgtcgcctcg acgatctcca tgccgagcgt ggtgagcagg gtttccttct ggtgcctggg
      601 gaacgggctc atgcgctgtt tcctcgtcaa cggcgaagga ttcggttctc gcgggccggc
      661 gagcgtacca gctcggggtc gcgcagcagg gacttggcgg tggtcgcggt gccggcgggg
      721 caggcgccgg tggccaggag ttccagcgcg gcggcgaagc gcccctcggc agggtcgccc
      781 agcggccgcg tgaaatcgtc ggccacggtg caggtgggcg caaagccgtc ggcgtaatcg
      841 ccgaaatccc tgtcgttcaa gccctcgaac tggatcgcga agtaggtcgt gccgcagttg
      901 tcctggggca ggaagccgta gggcttgccg caggtggttc cgcccacgag gttcacctcg
      961 acgtccacgc cgcgcagcgc attgatcacc gactcgctcg ccgaacacgt gcccgagctg
     1021 gtgaggaccg tcacgcgcgc gaggctcagc gtcggcagcg cagtgcctgc ggctatcgag
     1081 aaaccctgcg tggtggcgtg gaagggcgtg acgaggcccg cgggatcgta gccgaacggg
     1141 ttcctgtcgt tgaagcgcag ccgctcgaag acccggcccg ccgtgcgttc cggccccgcg
     1201 atcatgtatg cgagctcggc ggcgatgtcg aggaaaccgc cgccgttgta gcgcatgtcc
     1261 agcaccagct cggaagcgcc cgcgtcgcgc agttgcccga ccgcctcggc gagggcccct
     1321 tccgccgtgg cgatgtggtc ggtgaagagc aagtagccca cgcgccccgc gggcgtgtcg
     1381 accgtgcgca cgtcctgcac gggaactttc gtgacggcga tcgcggccag ggtcaccgtg
     1441 cgcggcgccg tcgctccggg gtcgcgaaag acgaagatcg tctgcttgcc cgcccggtcg
     1501 gggaatgcgc cggcgttgat cgccgccgcg tccgcgccat tcacgaaatc gacgccgtcc
     1561 acggagacca cttccgcgcc gcgcacgatt cccgcattgg ctgccggaga ccccgggtcg
     1621 acgttggcca ccaccatgcg tcgcggcggc gagcgcgcaa gcagcgccca ctcgatgccg
     1681 tagcccgcct ccacgccggc gagcgaaagc tggttccacg ccgtggtgtc gaagacgaag
     1741 tggaagcggt cccggggatt gccggaggcc gtcgtcgccg gtgtccgcag gacattgaaa
     1801 tactcgaccg gtcccgcgta cgccgcggcg ggcgggtcgg gcacctcgcg gtaccagagg
     1861 taggtttcgt cgatccacga gcgcacccag cgtttctcgt cgtcgagcga gccctgcaag
     1921 tcgggatagg ggcgattgcc gtgaaacggg tcggtgccgg ggcgcggtac ggcacagtgc
     1981 gcggcgaggc cggccgaagg cgggaaagac cgcgtcggcg gcaagggtgt cgcgtcggcc
     2041 ggcgagccac ctccgcctcc gccgcaaccg gcaacggcag cgaagagcag cgccgcgatc
     2101 gcgtggcgcg gggagtgcat cgacaggcgc atggagcgca atcttacctt caccacccga
     2161 tgccgtagtc ctcgccgtgg ttgctggagg cgccccacaa ggtgccgtgc cggcggtcga
     2221 tcatgatcgc gttcaccgga ccggaggtgc ggtcgcggta gatcgggtcg tagcccatgg
     2281 cgcgcagttc cttgcgcacg tacggcggcg tggcatcgtt gagcatgatg cgccccgggc
     2341 gcgactcgtg cgcctcgaag gaggcgcgca tctggtagct gttgaatccc ggcgcctcgg
     2401 cggcctcctg cggggtcatg ccgaactcga ccacgttcag gaagaactgc agcaggtcct
     2461 ggtcctgccc gtcgccgccc tgcacggcga acgcgaggta cggttgcccg tccttgagcg
     2521 cgaggctggg cgtgagggtg acgcgcgggt attttccggg ggccaccacg ttgaacggat
     2581 tttccgccgg gtccatcacg aagctctgca tgcgctgcga aaggccgatg ccggtcttgc
     2641 cggcgatgac tgcgggcacc caaccgccgc tcggcgtcac cgacaccacc cacccttcct
     2701 tgtccgcggc gatgacggac gtcgtgcccg agtagaaggc gcgctcgaag gcggcgtcgt
     2761 tggcggccat cggggtgccg cccttgcccg gttccttctt gcgcggctcc tgccagcttt
     2821 tcaggagatc gaggtacgga ttcgccgcgc gctggaacgg atacgggtcg ccgggcgcga
     2881 ccttcgggtc gttcttcatc gggtcgacga gcttggcgcg ttggcgcgcg tactccttcg
     2941 acaggagtcc cgccaccggc tcctcgggcg gggtgtaggt gtcgccgtag tagaagtcgc
     3001 gatcggcata ggcgaggttc atcgcctggt acacggtgtg gatgtaacgc gtgctgttgt
     3061 agcccatcgc cttgaggtcg aagttgccga ggatgttcag cgcctgcagc atcaccgggc
     3121 cttgcgtcca ggcggttagc ttgtagacgt cgatgccctt gtagctcgtc atcaccggct
     3181 cctcgaggcg gaccttccag ccggcgaggt cggcttcggt gaacaggccg ccctgcgcgc
     3241 gcacgctcgc cacgaggtct cttgcgatgt cgccgcggta gaagcgatcc tgcgcggctt
     3301 cgatcgcctg ccggcgtgtc ttcccggcgg ccagcgcggc cttttcggcc tccacgagct
     3361 tgcgcagggt cgcggcaagg tccggctggc ggaacatctc gcccgcttcc ggcgcgtggt
     3421 cggccgcccc cggcaacatg atccggcgcg aatccggcca ctgcatcagg tgcgcgcgct
     3481 ggcgctcgat gtagccggcc agttgcgcct cgatcggata gccgtcggcc atcgcgatgg
     3541 cgggttccag cacttccgcg agggaaagct tgccgaactc cgcgagcatc gtgaggatgc
     3601 cgccgggcgt gcccggcgtg acggcggcga gcggtccata gggcggcggg tagtccaggc
     3661 ccttcgcgcg gaagaattcc ggcgtggccc cggcgggcgc gacgcccagc gcgttgatgc
     3721 cgatgacctt cttcgtatgc gggttccaga cgagggcctg cgtctcgccg ccccagtgca
     3781 gcgtgtccca catcgttgcc gtggcggcga tcatggcgca ggccgcatcg accgcgttgc
     3841 cgcccttgag gaacaccttc gcgcccgcgg cggcacccag gggcttgccg gtgaccgcca
     3901 tccattcctg tccgtgcagc ggcggcttct gcgtgcgctg cgcgatggcc gtggcgggaa
     3961 ggccggcgat ggcgatggcg agaacgaggc ggtagaacat gaggcgaact cctgaatccg
     4021 gttcgggtta gcgggcagcc ttttcgcgcg cgatccactc gtcgatgcgc gcgtcgagca
     4081 ggttgagggg cagcgcgccg tcgtcgagga cggcattgtg gaagcggcgc aggtcgaagc
     4141 gctcccccag cgcctgctgc gctttcgcgc gcagcgcctt gatccgcaac tcgccgatct
     4201 tgtagccgag cgcctggccg ggatcgagga tgtagcgatc gacctcggcg atggccgcgc
     4261 tttcgtcgat gcccgaggtg tcggccaggt agcggatcga ctgctcgcgt gtccagccga
     4321 acgcgtgcag ccccgtatcc accacgagcc ggcaggcccg ccacatttcc atggagaggc
     4381 gcccgaactt cgagagggga tcggcgtaca ggcccatctc ctcgcccagg ctttccgcgt
     4441 agagcgccca gccctcgccg taggccacgt agaagctggc gcggcgaaac gacggcaatc
     4501 ccttcaactc ctgcgcccgc gccgtctgca ggtgatggcc gggcaccgcc tcgtgcagca
     4561 gcaccgcctc catgtcgtgc ctggcacggc gcgacaggtt gttggtgttg gcctcgaaga
     4621 agccggcgcg gctgccgtcc agcgcgcccg gcgtgtagtg ctccgcgttg tcgcccaggt
     4681 aggccggcat cggccggatt ccgtatggca gccgcggcag ctccgcgaag aagcggggca
     4741 attcggcgtc cgcgcgcttg gcgatgtccc ggtaatgctc cagcatcgaa tgcgcactcg
     4801 tgtggtagga acgcggattc gtcttgatcg attgcacgaa cgccgcgaac gaacccttga
     4861 atccggtcga tgcgatcacc cggtccatct cggcgcggat gcgcacgact tcgcgcttgc
     4921 cgatctcgtg aatctgcgcc gcgtcgagat ccgtggtcgt catcgtcgcg atcatcatcc
     4981 ggtagtaggc cgcgccggcg ggaagcgtcg atgcggcgag gtcgtcgcgg caggcgggca
     5041 ggtactcctc ttccacgaag cgccggaacg ccgcgaacgc gggttggacg cgctgcgcga
     5101 gggtaatcct tgcggcctct gccaggcgcg cgcggtcctg cgcggccacg gccgccggga
     5161 actggcggaa cggcgcatag agcggcgtgg ccgtcgcgtc gccgccggag aagaccgcga
     5221 gctgccccgg cacctgcgac atcgcggccc gcggtggcat ccagcccgac tcgatgccgg
     5281 cctccatgac aacgcgcgat tcggcgagga cgcgcggcac ggcctccagg cgcttgagat
     5341 agttctcgta gtcgcgcgcg tcgcgaaagg gcgcggcccg ggccagcatg ggaaggatca
     5401 cctgcggtcc ggacgaaggc gagacctgca tccacccatc gccgcgcccg gcgccgaacg
     5461 gcaggtcgcc gaagagcgcg ttgagctcat cgcgcctgcg cagggagtcc ttcatcatct
     5521 ccagcgagat gcggtcctgc gtggaaagcc gcttcgggtc gaagcgcccc agatcggcca
     5581 gcaccgcctt cacgtgcgcc ttgcgcctcg cgaccgcctc cggcgacccg tcgtgcaggc
     5641 ggtcgttgaa cgcgggattg ccgatcatcg tcgcgaactc gggaaactct tcctggccgc
     5701 gcaggaactc gcgctcgaag agggcgtgca gcgccggttc gggtgc
//
LOCUS       DASUEV010000005         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_917883, whole genome shotgun sequence.
ACCESSION   DASUEV010000005 DASUEV010000000
VERSION     DASUEV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917883"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      169..264
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      299..402
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            455..1537
                     /gene="gcvT"
                     /locus_tag="VFV55_00130"
     CDS             455..1537
                     /gene="gcvT"
                     /locus_tag="VFV55_00130"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX4942726.1"
                     /translation="MPKATPLNAVHREAGARMVDFGGWDMPVHYGSQVEEHHAVRRDA
                     GLFDVSHMRVVEIEGAGAREFLRFALANDVAKLTVPGKALYSCLLNDSAGVVDDLIVY
                     FFRDDFFRLVVNAGTAEGDIQWLRSLNALAGHGVAITPREDLAMIAVQGPNARARFWS
                     AFPETKPATEDLVPFSAARVGDIMVARTGYTGEDGFEVMFPAEDAVSIWKGCVAAGVR
                     PCGLGARDTLRLEAGMNLYGQDMDAATSPLDAGLAWTVDLKSPRPFYGRKVLEGRPRK
                     ADFLGLVLIDKGGVLRAHQKVLAEGGEGEITSGTFSPTLGISIALARLPIGSAPGATV
                     RVVVRDKQLAARIVKPPFVRNGKPLI"
     gene            1595..1975
                     /gene="gcvH"
                     /locus_tag="VFV55_00135"
     CDS             1595..1975
                     /gene="gcvH"
                     /locus_tag="VFV55_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX4942727.1"
                     /translation="MNIPQELKYTQSHEWARREADGTVAVGITDHAQEQLGDIVFVEA
                     PKPGRKVAAGEAVGVVESVKAASDIYAPIAGEIVAGNDELANAPEKVNADAYGCWMYR
                     IRPDSAADLDKLLDAAAYEKIASE"
     gene            1985..>4253
                     /gene="gcvP"
                     /locus_tag="VFV55_00140"
     CDS             1985..>4253
                     /gene="gcvP"
                     /locus_tag="VFV55_00140"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX4942728.1"
                     /translation="MPDTTVSPPAARATLDQLEDRAAFARRHVGPDAKDQADMLAALG
                     FASRDALIAAIVPAAIRRKSPMALPVGKPEAEALAELRAVAAKNRVFRSWIGQGYYGT
                     HTPGVILRNILENPAWYTAYTPYQPEISQGRLEALVNFQTMICDLTAMAIANASMLDE
                     ATAAAEAMTLCRRSGNHASSVFAVASDVLPQTLDVLRTRAAPLGIEIRVAAPAELEGV
                     NAFAALVQYPGLDGEIRDYRALAESLHAKGGHLVVAADLLALAVLVPPGEWGADVVVG
                     SAQRFGVPMGYGGPHAGYLATRDALKRSMPGRLVGVTVDAHGNPAYRLALQTREQHIR
                     REKATSNICTAQVLLAVMASMYAVYHGPEGLRRIARRVHRLTAIAREGLVKLGFEVTA
                     GAFFDTITVKSGAATAAIVARTAQAKSNLRRVDGATLGFSFDETTTREDVASLWAIFS
                     GAPAPFAPDDLDAVAEGIPATLARTSAFLAHPVFNRYHAETEMMRYLRRLADRDIALD
                     RSMIPLGSCTMKLNAASEMIPVTWPEFGNLHPFAPAEQAEGYRELIAGLEKMLVECTG
                     YTAVSMQPNAGSQGEYAGLLVIKAWHESRGEGHRNVCLIPSSAHGTNPASAQMAGMRV
                     VVVACDSQGNVDLADLRAKAEAHRADLAAIMVTYPSTHGVFESGIRDICDIVHAHGGQ
                     VYVDGANLNALVGLASPGHFGADVSHLNLHKTFCIPHGGGGPGVGPVAVAAHLKPFLP
                     GHRCLDRTMQAIGAVA"
BASE COUNT          672 a         1474 c         1469 g          638 t
ORIGIN      
        1 cgcgtccctc gagcgccgat gtcttttccg ggcagtgccg gtggcgggcc aacggccatg
       61 ctaggccgtg cacggctgcg cccattgcgc tcgatcaatg ggcatgcgcg cgagtgagcg
      121 aaggccgggt cgcgttgaca atcaccgggt gcccgcgtag cctccggcgg catccggtcg
      181 ggagagcgtc gccgcgttcg ttcgcgtcgg ccgccgaagg ggtagcgccc gcaaactctc
      241 aggcaaaagg accgaccgga gccgggttcg cgtctccgca tggcggagcg acgaggcagc
      301 cccggactct ggagagcgac ggcacgaccg tccaccgaag gggctcgcgg cctggggaac
      361 cggcgccgta atctctcagg tacaagggac agaggggcca ggcaagtgat gctacgcttg
      421 cctggccctt tttcatttcc gcgagggagt cgccatgccg aaagccacgc cgctcaacgc
      481 cgtccatcgc gaggcgggcg cccgcatggt ggatttcggg ggctgggaca tgcccgtcca
      541 ctacggctct caggtcgagg agcaccacgc ggtgcgacgc gacgcggggc tcttcgacgt
      601 ctcgcacatg cgcgtggtgg aaatcgaggg cgcgggcgcg cgggagttcc tgcgtttcgc
      661 cctcgccaac gacgtggcga agctcaccgt gcccggcaag gcgctctact cgtgcctgct
      721 caacgattcc gcgggggtgg tggacgacct catcgtgtat ttcttccgcg acgacttctt
      781 ccgcctggtg gtgaatgccg gaaccgcgga aggcgacatc cagtggctcc gctcgctcaa
      841 tgccctcgcg gggcatggcg tggcgatcac gccgcgcgag gatctcgcga tgatcgcggt
      901 gcaggggccg aatgcgcgcg ccaggttctg gagcgcgttt cccgagacga agccggctac
      961 cgaggacctc gtgcccttca gcgcggcgcg cgtgggcgac atcatggtgg cgcgcaccgg
     1021 atacacgggc gaggacggct tcgaggtgat gtttcccgcg gaggacgcgg tcagcatctg
     1081 gaagggctgc gtcgcggccg gggtgcggcc ctgcgggctc ggcgcgcgcg acacgctccg
     1141 gctcgaggcg ggcatgaacc tctacgggca ggacatggac gctgccacct cgccgctcga
     1201 tgccggcctt gcatggaccg tggacctcaa gtcgccgcgg cccttctatg gccgcaaagt
     1261 gctcgagggc cggccgcgga aggccgattt cctcgggctc gtgcttatcg acaagggcgg
     1321 cgtcctgcgc gcgcaccaga aggtgctggc cgagggcggc gaaggcgaga tcaccagcgg
     1381 caccttctcg cccacgctcg gcatttcgat cgctcttgcg cgactgccga tcggctccgc
     1441 cccgggcgcc acggtgcgcg tggtggtgcg cgacaagcaa ctcgccgcca ggatcgtgaa
     1501 gccgcccttc gtgcgcaacg gcaaacccct catatgaaaa tgtacccggt acatgtaccg
     1561 ggtacaggat ttggaacccc acggagaaaa tgccatgaac attccccagg aactcaagta
     1621 cacgcagtcg cacgagtggg ctcgccgcga ggccgacggc actgtcgcgg tcggcatcac
     1681 cgaccacgcg caggagcaac tgggtgacat cgttttcgtc gaggcgccca agccgggccg
     1741 caaggtggcg gcgggcgaag ccgtgggcgt cgtcgagtcg gtgaaggccg cctcggatat
     1801 ctacgctccg attgccggcg agatcgtggc cggaaacgac gagctcgcca acgcgccgga
     1861 aaaggtgaac gcggacgcct acggctgctg gatgtaccgc atcaggccgg acagcgcggc
     1921 cgacctcgac aagctgctcg acgcagccgc ctacgagaag atcgcctccg agtaaggaac
     1981 cgccatgccc gacacgaccg tctcgccgcc cgccgcccgc gccacgctcg accagctcga
     2041 ggatcgcgcc gccttcgcgc gccgccacgt tggccccgat gcgaaggacc aggccgacat
     2101 gctcgcggcc ctgggcttcg cctcgcgcga cgcgctcatc gcggccatcg tgccggcagc
     2161 cattcgccgc aagtcgccca tggcgctgcc tgtgggcaag cccgaggccg aggcgctcgc
     2221 ggaactgagg gccgtcgccg cgaagaaccg cgtcttccgc tcgtggatcg ggcagggcta
     2281 ctacggcacg cacacgccgg gcgtcatcct gcgcaacatc ctcgagaacc ctgcgtggta
     2341 cacggcgtac acgccctacc agccggagat ctcgcagggg cggctggagg ccctggtcaa
     2401 cttccagacc atgatctgcg acctgaccgc gatggcgatc gccaatgcgt cgatgctgga
     2461 cgaggcgacg gccgccgcgg aagcgatgac cctctgccgg cgctcgggca atcatgcctc
     2521 gtccgtcttc gccgtcgcct ccgatgtgct gccgcagacg ctcgacgtac tgcgcacgcg
     2581 ggcggcaccg ctgggcatcg aaatccgcgt cgcggcgccg gcggaactcg agggcgtgaa
     2641 tgcatttgcc gcgctcgtgc agtatcccgg tctcgacgga gagatccgcg actaccgcgc
     2701 gctggccgaa tcgctgcacg cgaagggtgg gcatctcgtc gtggccgcgg acctgctggc
     2761 ccttgcggtg ctggtgccgc cgggcgaatg gggtgcggac gtcgtggtcg ggtcggcaca
     2821 gcgattcggc gttccgatgg gctatggcgg cccgcacgcg ggctatctcg cgacccgcga
     2881 tgcgctcaag cgcagcatgc cggggcgcct ggtcggcgtg acggtcgatg cgcacggcaa
     2941 cccggcctac cgtctcgcgc tgcagacgcg cgagcagcac atccgtcgcg agaaggcgac
     3001 gtccaacatc tgcaccgcgc aggtcctgct ggccgtgatg gcctccatgt acgcggttta
     3061 ccacgggccc gaaggtctcc ggcgcatcgc gcgccgcgtg caccgcctca cggcgatcgc
     3121 ccgggaaggg ctcgtgaagc tcggcttcga agtgacggcg ggggctttct tcgacaccat
     3181 caccgtgaag tcgggtgctg cgacggccgc catcgtggcg aggaccgcgc aggcgaagtc
     3241 gaacctgcgg cgcgttgacg gcgccacgct cggcttctcc ttcgacgaaa cgacgacgcg
     3301 cgaagacgtg gccagcctgt gggcgatctt ttcgggcgca ccggcgccgt ttgcaccgga
     3361 tgacctcgac gcagtcgcgg agggcatccc cgccacgctg gcgcgcacca gcgccttcct
     3421 cgcgcacccg gtgttcaacc gctatcacgc cgagacggag atgatgcgct acctgcgccg
     3481 cctcgcggac agggacatcg cgctcgaccg gtcgatgatc ccgctgggct cgtgcacgat
     3541 gaagctcaat gcggcgagcg agatgatccc ggtgacgtgg cccgagttcg gcaacctcca
     3601 tcccttcgcg ccggcggagc aggccgaagg ctaccgcgag ctcatcgcgg ggctcgagaa
     3661 gatgctcgtc gagtgtaccg ggtacaccgc cgtgtcgatg cagcccaacg cgggatcgca
     3721 gggcgagtac gcggggctcc tcgtcatcaa ggcctggcac gagagccgcg gggaggggca
     3781 ccgcaacgtc tgcctcatcc cgagttcggc gcacggcacc aatcccgcgt ccgcgcaaat
     3841 ggccggcatg cgggtggtcg tcgtcgcgtg cgacagccag ggcaacgtcg atctcgccga
     3901 cctacgcgcc aaggcggaag cgcatcgcgc cgatctcgcc gcgatcatgg tcacctatcc
     3961 ctccacgcac ggcgtgttcg agtccggcat ccgcgacatc tgcgacatcg tgcacgcgca
     4021 cggcgggcag gtgtacgtcg acggcgccaa cctcaacgcg ctggtcggcc tcgcctcgcc
     4081 cggccacttc ggcgccgacg tttcgcacct caacctgcac aagacgttct gcattccgca
     4141 cggcggcggc ggccccggtg tcggaccggt cgcggtcgcc gcgcacctga agcccttcct
     4201 gccggggcac cgctgcctcg accggacgat gcaggcgatc ggcgccgtcg cgg
//
LOCUS       DASUEV010000006         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_688921, whole genome shotgun sequence.
ACCESSION   DASUEV010000006 DASUEV010000000
VERSION     DASUEV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688921"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFV55_00145"
     CDS             complement(<1..1147)
                     /locus_tag="VFV55_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942729.1"
                     /translation="MHPHSPPATIITSRWGSLGDLATAAFVIAAVSGVAVAVPYDPAD
                     GYGSIAAMLLANPAAVFFRNLHYWAGQACLLLALAHAWDRLRVQTGNRVSRGPWLRLA
                     LTLPILAFIMLSGFMLRGDADARQALRIVTEATAQVPFIGPLLATLLFGAAERLDLVY
                     VQHAATATIIVWLFIIEHARRVWPRAAALGAVALATGVVSLFLSPGLHDGVDPIVKGP
                     WYFLGLQEILHWTPWPLVVVLAGIVAVSALYALRAMRPGRAAGTKAVLLAILVTYAGL
                     CAVGMFLRGENWSFAPAWPTGAGNPRAGFVFAATPDVPAPLPVAMGRPEGCLVCHRGV
                     TGLGDAHRPEAVGCASCHGGDVLTLDRTRAHAGMELIPGNLATAMGRC"
     gene            complement(1134..1493)
                     /locus_tag="VFV55_00150"
     CDS             complement(1134..1493)
                     /locus_tag="VFV55_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX4942730.1"
                     /translation="MAVGLPFAGLLVAMLRRVRARQLPGAVRIPPDVAMGLSVIEGAI
                     VHRDIGGVPRAFSARCTHLGCRIDRVAGDEVVCPCHGSRYRSDGTVAAGPATRALAPM
                     RIEPDPASGGWIARASS"
     gene            1722..2660
                     /locus_tag="VFV55_00155"
     CDS             1722..2660
                     /locus_tag="VFV55_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX4942731.1"
                     /translation="MNRKLSLVLAVVAALAAGAAQAQTIRLMTGPQGGVWVPLGGALK
                     NMWEKAIPGLQVQTLPGAGIANIRGVDEGKADVGFGNSITSVDGVNGRAPYPHKVTKA
                     CQLANLYPQYFQVVALADSGIKGIADLKGKSIAVQPKGNTAELISQQILQSVGLSYQS
                     AKVSFVNSYTDAAALLKDGHAQVFTLGTTIPASSVMDVASGRDIKMVPVTDEIVAAMR
                     KMNPGYIKGAIKGGTYPKQDQDVPAIVYSAHLVVSCSLPDQQVYQMAKAMAANVADMA
                     AINKAMTGLTPKMMAEDIGVPFHPGAAKFYKEVGAM"
     gene            2661..4553
                     /locus_tag="VFV55_00160"
     CDS             2661..4553
                     /locus_tag="VFV55_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX4942732.1"
                     /translation="MARTGGAVQMGTVIRWAAGGIAIAMSLYHMWFAASAPPEAMIFR
                     GTHLLFALVLVFLFVPAWRSEGPRRFLWLDIALVAASALAIGYIFVTYQSIVNRIIYI
                     DELTAWDKAMAVLTVVLVLDATRRVIGWALPITALIFLGYAVFFSGVKMPILLEQMYL
                     STDGIFGSTLGVSASYVIVFVLFGSFMEKSGTGRLFMDFALSLTGHTAGGPGKVAIVS
                     SSLFGTISGSAVANVMVDGPITIPLMKKTGFRPPFAAAVEASASTGGQIMPPIMGAAA
                     FVMAEYLAVSYFQVTVWAWIPALLFYVALFFAVHFEAKRSGLVGVPRAELPRLGRVMR
                     ERGHLFVPILVVLGGMAMSYSAPLCALAGTIATLPVALLRRSTREGIVWRTVLEALEG
                     GARDALSVAMACACAGIVIGVISLTGLGITFTQLVLALAQNSLILALVLTAVAGIVLG
                     MGMPTTPAYIVMVSLLVPALIKLGVVVPAAHMFAFYFAVLSAITPPVALAVFAAAALA
                     KANMWRAGFESVRIAAAGFIVPFMFVYEPALLAIGDWGTIALASATATIGVMALAASL
                     YGYLLAPLALWQRVTLAAAAFLLIKPGFVTDIAGLCLLGLVVALQWGASRRAVAARTL
                     RPEAGK"
BASE COUNT          758 a         1633 c         1632 g          736 t
ORIGIN      
        1 cgcagcggcc cattgcagtc gccaggttgc cggggatcaa ttccatgccg gcgtgcgccc
       61 gcgtcctgtc gagcgtcaat acgtcgccgc catgacatga cgcgcagccg acggcctccg
      121 gccggtgcgc gtcgcccaag cccgtgacgc cgcgatgaca gaccaggcac ccctcgggcc
      181 gtcccatcgc gacgggaagc ggcgccggca cgtccggcgt cgccgcgaat acgaaaccgg
      241 cacgcggatt tccggctccg gtgggccacg ccggcgcgaa cgaccagttc tctccgcgca
      301 ggaacatccc cacggcgcac aagcctgcat aggtcacgag gatcgcgagc agcaccgcct
      361 tcgtgccagc cgcacggcca ggtcgcattg cgcgcaaggc gtagagggca ctcacggcga
      421 cgatcccggc aagcaccacc acgagcggcc atggtgtcca gtgcaggatc tcctgcagcc
      481 cgaggaagta ccacggccct ttcacgatcg ggtccacgcc atcgtgcagg cccggcgaca
      541 ggaagagcga gacgacgccg gtggccagcg ccaccgcccc tagtgccgcc gcgcgcggcc
      601 atacccgtcg cgcatgttcg atgatgaata gccagacgat gatcgtcgcc gtcgcggcat
      661 gctgcacgta gaccaggtcg aggcgctcgg ccgccccgaa cagcagcgtc gcgagcagcg
      721 gtccgatgaa aggcacctgc gccgtggcct cggtcacgat gcgaagcgcc tggcgtgcgt
      781 ccgcgtcacc gcggagcatg aaccccgaca gcatgatgaa ggcgagaatc ggcagtgtca
      841 gggcaagccg cagccacggg ccccggctga cgcgattccc ggtttgcacc cggaggcggt
      901 cccacgcatg cgccagcgcg agcaacaggc aggcctggcc ggcccagtag tgcaggttgc
      961 ggaagaacac cgccgcggga ttggccagga gcatggcggc gatggacccg tacccgtcgg
     1021 cagggtcgta gggcaccgcg acggcaacgc ccgataccgc cgcgatcacg aaagcggcgg
     1081 ttgccaggtc gccaaggctg ccccagcgcg aggtgatgat cgtggccggg gggctatgag
     1141 gatgcacggg cgatccatcc cccggatgcc ggatcgggct cgatgcgcat cggtgcgagc
     1201 gctcgtgtcg ccgggccggc ggcgaccgtg ccatcggagc ggtagcgcga gccgtggcat
     1261 gggcacacca cttcgtcgcc ggccacgcgg tcgatccggc agccaaggtg ggtgcaacgt
     1321 gccgaaaacg cgcgcggtac tccgccgata tcccggtgca cgatggcccc ctcgatgacg
     1381 gagagcccca tggcgacgtc gggcgggatg cgcactgcgc cgggaagctg gcgggcccgc
     1441 acgcgccgga gcatggccac cagcaagccg gcgaacggga gaccgaccgc catcgccagg
     1501 gcttgccgga gaacccgccg ccgggacaac gagggcgcga gcccgaagct cataggatgc
     1561 ctgaccacca cgcaggcccg aggtgagcct cgtggggatt ctaccccgag gcacgcacgc
     1621 tgaaacgcat caatgcgcgg gccctcgggc ccgtgcgata ctccggcggt caagtcccgg
     1681 cccgacacgg ggcgacattc cgacaaccgg aggagaaaat catgaaccgc aagctttccc
     1741 ttgtgctggc cgtcgttgcg gccctcgctg ccggcgccgc ccaggcgcaa acgatccgac
     1801 tgatgaccgg cccgcagggc ggggtctggg tgccgctggg cggcgcgctg aagaacatgt
     1861 gggaaaaggc cattcccggc ctgcaggtgc agacgcttcc gggggccggc atcgcgaaca
     1921 tccgcggcgt ggacgaaggc aaggccgacg tgggcttcgg caacagcatc acctcggtcg
     1981 atggcgtcaa cgggcgcgcg ccatacccgc acaaggtgac caaggcctgc cagcttgcga
     2041 acctctatcc ccagtacttc caggtggtgg cgctcgccga cagcgggatc aagggcatcg
     2101 cggacctgaa gggcaagagc atcgcggtgc agcccaaggg caacaccgcc gagctcatct
     2161 cgcagcagat cctgcagtcg gtcgggctct cctaccagag cgcgaaggtg agcttcgtga
     2221 acagctacac cgacgccgcg gcactcctca aggacggcca cgcgcaggtg ttcaccctgg
     2281 gcacgacgat ccccgcttcc tcggtgatgg acgtggcctc cggccgggac atcaagatgg
     2341 tgccggtgac cgacgagatc gtggcggcga tgcgcaagat gaatcccggc tacatcaagg
     2401 gcgcgatcaa gggcggcacc tatccgaagc aggaccagga cgtgccggcg atcgtctatt
     2461 cggcgcacct ggtcgtctcc tgcagcctgc ccgaccagca ggtctaccag atggcgaagg
     2521 cgatggccgc caacgtcgcc gacatggccg cgatcaacaa ggcgatgacc ggcctcacgc
     2581 cgaagatgat ggcggaggac atcggcgtgc cgttccaccc cggcgcggcg aagttctaca
     2641 aggaagtggg tgcgatgtaa gtggcgcgga cgggcggcgc agtgcagatg ggcacggtga
     2701 ttcgctgggc ggcgggcggc atcgcgatcg cgatgtcgct ctaccacatg tggttcgccg
     2761 cctcggcccc gcccgaggcg atgatcttcc gcggcacgca cctgctcttc gcgctcgtcc
     2821 tcgtcttcct gttcgttccg gcgtggcgca gcgaaggccc gcgccgattc ctctggctgg
     2881 acatcgcgct cgtcgccgcg tcggcgctgg cgatcggcta catcttcgtc acctaccagt
     2941 cgatcgtgaa ccgcatcatc tacatcgacg agctcaccgc gtgggacaag gcgatggcgg
     3001 tgctcacggt ggtgctggtg ctggacgcga cgcggcgcgt gatcggctgg gccctgccga
     3061 tcaccgcgct gatcttcctc ggctacgcgg tgttcttttc cggcgtgaag atgccgatcc
     3121 tgctcgagca gatgtacctc tccacggacg gcatcttcgg ctccacgctc ggcgtatccg
     3181 cctcgtacgt gatcgtgttc gtgctcttcg ggtcgttcat ggagaaaagc ggcacggggc
     3241 ggctgttcat ggatttcgcg ctctcgctca ccgggcacac ggcgggcggc ccgggcaagg
     3301 tcgcgatcgt gagctcgagc ctcttcggca cgatctcggg cagcgcggtg gccaacgtca
     3361 tggtcgacgg gccgatcacc atcccgctca tgaagaagac gggctttcgc ccgcccttcg
     3421 ccgcggcggt ggaggcgagc gcctcgacgg gcgggcagat catgccgccg atcatgggcg
     3481 cggccgcttt cgtgatggcc gagtacctcg cggtgagcta cttccaggtc accgtctggg
     3541 catggatccc ggcgcttctc ttctacgttg cgctcttctt cgcagtccac ttcgaggcga
     3601 agcgcagcgg cctcgtgggg gtcccgaggg cggagctgcc gcgcctgggg agggtcatgc
     3661 gcgagcgcgg ccacctcttc gtcccgatcc tcgtcgtgct gggcgggatg gcgatgtcct
     3721 acagcgcgcc gctgtgcgcc ctcgcgggga ccattgcgac gctgccggtc gcgctcctgc
     3781 gcaggagcac gcgcgagggc atcgtgtggc gcacggtact cgaagcgctc gagggcggtg
     3841 cgagggatgc gctctcggtg gccatggcct gcgcctgcgc cggcatcgtg atcggcgtga
     3901 tctcgctcac cgggctgggc atcaccttca cgcagctcgt gctcgcgctg gcgcagaaca
     3961 gcctgatcct ggcgctggtc ctcaccgcgg tggcaggaat cgtcctcggc atgggcatgc
     4021 ccacgacgcc ggcctacatc gtcatggtgt cgctcctcgt gccggccctc atcaagctcg
     4081 gcgtcgtcgt cccggcggcg cacatgttcg cgttctactt cgcggtgctg tcggcgatca
     4141 cgccgccggt ggcgctggcg gtcttcgccg ccgcggcgct cgccaaggcg aacatgtggc
     4201 gcgcgggctt cgagtcggtg cgcatcgccg ccgcgggctt catcgtgccc ttcatgttcg
     4261 tctacgagcc ggcgctgctc gccatcggcg actgggggac gatagccctc gcctcggcga
     4321 cggcgaccat cggcgtgatg gcgctcgcgg cttcgctcta cggctacctt ctcgcgccgc
     4381 tcgctctctg gcagcgcgta acgctcgctg cggcggcgtt cctcctcatc aagccgggct
     4441 tcgtcaccga catcgccggg ctgtgcctgc tcggactcgt ggttgcattg cagtggggcg
     4501 cgtcacggcg cgccgtcgcc gcccgcacct tgaggcccga ggcggggaag taggtcgcca
     4561 tcccgcgccc gcgggtcgaa gaggcgtcgg ggaaagtcga tcgcaatcct gggcgcgcaa
     4621 gcctgaccgt cggatgggtc ccaaaaatcc gaggccaggc aaaacaagcc aggcctcgga
     4681 tttttaccca tctaatgacg agggcacttc gctgcctcgt ccgcgacggt cgcgtgccaa
     4741 ggattgcgct cgactttcc
//
LOCUS       DASUEV010000007         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1032718, whole genome shotgun sequence.
ACCESSION   DASUEV010000007 DASUEV010000000
VERSION     DASUEV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032718"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFV55_00165"
     CDS             <1..807
                     /locus_tag="VFV55_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX4942733.1"
                     /translation="VEIESGIEGLVHVSEMDWTNKNVHPAKVVSLGDEVEVMILEIDE
                     DRRRISLGMKQCMPNPWEEFAMNHKKGDKVNGLIKSITDFGVFIGLPGNIDGLVHLSD
                     LSWNAAGEEAVKEMKKGEEVETVVLSIDVERERISLGIKQLEGDPFGNYVTIHDKGNI
                     VKGTVVSIESKGAVIELDKDVEGYLRASEISRDRVEDVRAHLKEGDEVEAVIVNIDRK
                     NRSINLSIKAKDAAEESAALQKMASESSASSGTTNLGALLKAKLGSGSKE"
     gene            847..1131
                     /locus_tag="VFV55_00170"
     CDS             847..1131
                     /locus_tag="VFV55_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX4942734.1"
                     /translation="MTKSQLIELLSARHSQLAPKDAELAVKTMLDAMAHTLASGNRIE
                     IRGFGSFGLNFRPPRVGRNPKTGEKVQVPRKYVPHFKAGKELRERVDSNE"
     gene            1276..1608
                     /locus_tag="VFV55_00175"
     CDS             1276..1608
                     /locus_tag="VFV55_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX4942735.1"
                     /translation="MQALAWIVRIAILLVLVWFAVRNGQEVELRGLPDQSWKAPLIFV
                     VLVAFVAGVIIGLTAWLPTVVRQRREVGRLRKAASTATATVAAAVPVAPAPEPTILPA
                     VRPDSDGV"
     gene            1598..>2069
                     /locus_tag="VFV55_00180"
     CDS             1598..>2069
                     /locus_tag="VFV55_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4942736.1"
                     /translation="MEFEFWWLLALPVFFGMGWIAARVDLKSLLLESRQMPSSYFRGL
                     NFLLNEQPDKAIESFLQVAKENPQTVELQFALGSLFRRRGEVDRAIRMHQDLIAREDL
                     PEEERRTASFELAEDYFKAGLLDHAESVLVKLAEAAPSPEVHRHLLDIYIQEKDW"
BASE COUNT          392 a          675 c          670 g          332 t
ORIGIN      
        1 gtcgagatcg agagcggcat cgagggcctg gtgcacgtct ccgagatgga ctggaccaac
       61 aagaacgtcc accccgccaa ggtcgtctcg ctgggcgacg aggtggaggt catgatcctc
      121 gagatcgacg aggaccgccg ccgcatctcc ctcgggatga agcagtgcat gcccaacccg
      181 tgggaagagt tcgccatgaa ccacaagaag ggggacaagg tgaacggcct catcaagtcc
      241 atcaccgact tcggcgtatt catcggcctg ccgggcaaca tcgacggcct ggtgcacctc
      301 tccgacctct cctggaacgc cgccggcgag gaagccgtca aggagatgaa gaagggcgag
      361 gaagtcgaga ccgtcgtcct gtcgatcgac gtcgagcgcg agcgcatctc gctcggcatc
      421 aagcagctcg agggcgaccc gttcggcaac tacgtcacga tccacgacaa gggcaacatc
      481 gtgaagggca ccgtggtctc gatcgagtcc aagggcgccg tgatcgagct cgacaaggac
      541 gtcgagggct accttcgcgc ttccgagatc tcccgcgacc gcgtcgagga cgtgcgcgcc
      601 cacctgaagg agggcgacga ggtcgaggcg gtgatcgtga acatcgaccg caagaaccgc
      661 tcgatcaacc tgtcgatcaa ggccaaggac gccgccgagg aatcggccgc cctgcagaag
      721 atggcctcgg agagcagcgc gagttccggc accacgaacc tcggcgccct gctcaaggcg
      781 aagctcggca gcgggtccaa ggaataggga agtcacaaga aaatgcacgg gggagactcg
      841 cacgccatga ccaagtcaca gctcatcgag ttgctgagcg cgcggcatag tcaactggcg
      901 cccaaggacg cggaactggc ggtgaagacc atgctcgacg cgatggcgca taccctcgcg
      961 tcgggcaacc gcatcgagat ccgcggtttc ggcagtttcg gcctcaactt ccgccccccg
     1021 cgggtggggc gcaaccccaa gacgggagag aaggtgcagg tgccgcgcaa gtacgtgccg
     1081 cacttcaagg ccggcaagga actgcgggag cgcgtcgatt ccaacgaatg aacggcctcg
     1141 gccggcgtga aagcgtcaag cggcagggta aaatgcaccc ctgccgcttt tttttcgtcc
     1201 tgactgccat cccgatcacg gctcgttgcc cgaccctttc ccactgaccg cccgttcccc
     1261 gaccacggcc acgacatgca agcgctcgcc tggattgtcc gaatcgcgat cctgctcgtg
     1321 ctggtctggt ttgcggtgcg caacggccag gaggtcgagc tgcgcggcct tcccgaccag
     1381 tcgtggaagg cccctctcat cttcgttgtc ctggttgcct ttgtcgccgg cgtgatcatc
     1441 gggcttaccg catggctgcc cacggtcgtg cgccagcgcc gcgaggtggg gaggctgcgc
     1501 aaggcggcgt caacggcgac tgccaccgtt gctgctgccg ttccggtggc ccccgctccg
     1561 gagccgacca tcctcccggc cgtgcgaccg gattccgatg gagtttgaat tctggtggct
     1621 cctcgcgctg ccggttttct tcggcatggg ctggatcgcc gcgcgcgtgg acctgaagag
     1681 cctgctgctg gaatcgcggc agatgccctc gtcgtacttc cgcggcctca acttcctgct
     1741 caacgagcag cccgacaagg cgatcgagtc gttcctgcag gtcgcgaagg agaacccgca
     1801 gacggtggaa ctgcagttcg cgctgggcag ccttttccgg cgccgcgggg aagtcgaccg
     1861 cgccatccgc atgcaccagg acctcatcgc gcgcgaggat cttccggagg aggaaaggcg
     1921 cacggcctcc ttcgagctgg ccgaggacta cttcaaggca ggcctcctcg accacgccga
     1981 gtcggtcctg gtgaagctgg cggaggcggc cccgtcgccg gaggtccatc gccacctgct
     2041 cgacatctac atccaggaga aggactggg
//
LOCUS       DASUEV010000008         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_116288, whole genome shotgun sequence.
ACCESSION   DASUEV010000008 DASUEV010000000
VERSION     DASUEV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116288"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFV55_00185"
     CDS             complement(<1..1091)
                     /locus_tag="VFV55_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4942737.1"
                     /translation="MSSCHRIGRPWLAHYPPGVPADVDVSATPTLVHLIDEAVARHGA
                     REQAVFLGRSFTHGRVDRAGRHFAAWLASHDIGAGRRVAIMMPNVPQYAAAVFGVLRA
                     GATVVNVNPLFTARELAAQLADAEVAAIVVLENFAATLAQALQGLAPMRVAVAAAGDW
                     LGPVKGPVANFILRHVKKAVRPWAIEGAVRFDELLARGAKLPFAAPAIRPGDTALLQY
                     TGGTTGVSKGAMLTHANVVAAVLAADAWLSPAFAGERRVENPTMVCALPLYHIYALVA
                     CLFLGLRLGARNLLIANPRDLDALVRDLRRNSPHLFPAVNTLYNALLDHPGFGDIDFS
                     GLRVANSGGMAVQRAVADRWLARTGCPIV"
     gene            1149..1781
                     /gene="pdxH"
                     /locus_tag="VFV55_00190"
     CDS             1149..1781
                     /gene="pdxH"
                     /locus_tag="VFV55_00190"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX4942738.1"
                     /translation="MNIADLRKDYALKRLDETDVDADPFRQFHAWLAEALEAQALEPT
                     AMTLATADARGRPSARIMLLKALDDRGFVFYTNYASRKGEELAARPHAALTFFWKEVE
                     RQVRIEGGIEKVSAAESDEYFASRPLGSRIGAWASTQSATIESRQWLEGRVKAAEAQY
                     GDNPPRPPHWGGFRVIPDWLEFWQGRQSRLHDRIAYSRAGGGWKITRLSP"
     gene            1784..2452
                     /locus_tag="VFV55_00195"
     CDS             1784..2452
                     /locus_tag="VFV55_00195"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX4942739.1"
                     /translation="MRSPLGCHDPARLEAERLSAEALERSLDEALAGHDPREPLWIFA
                     YGSLMWNPGLAFVAKRVGTIYGFHRNFCLWSRINRGTPEHPGLVLTLERGGSCRGLAF
                     RLTGSTSRNELLALWRREMSMGSYHPRWLEFHAGADRLPVLAFIVNRSCSGYAGKLPV
                     DAMVQSIATARGKFGSSAEYLFQTQAALESHGILDVQVKRLAERVKAYLAHGSGGAAA
                     HAPA"
     gene            complement(2460..2918)
                     /locus_tag="VFV55_00200"
     CDS             complement(2460..2918)
                     /locus_tag="VFV55_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX4942740.1"
                     /translation="MSPYALVATCIACVVAGCSTEPSRSPEEVDRIVAAADWERTTDV
                     KIELKDAGFTPRELRLKVGQPYRLTLVNVGVNNHYFNAPEFFATIAARKAEVPRYAEL
                     KAPHFSKFELYAAGGTMDLWFIPLEKGRYRAFCHLGNHAEMGVEGQLVVE"
     gene            complement(3018..4553)
                     /locus_tag="VFV55_00205"
     CDS             complement(3018..4553)
                     /locus_tag="VFV55_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4942741.1"
                     /translation="MPEWAKVPAAAPRRGKGGKGPRGGPDTSKRERLQKVMAQSGHGS
                     RRNIEILIAQGHIAINGQVAKLGDLVGPGDRVKLDGKLISLRFGSLLPRVLLYHKPEG
                     EICTRDDPEGRPTVFAKLPKVNNARWVSIGRLDFNTSGLLIFTTNGELANRLMHPRYE
                     IEREYAVRLMGELTPEQVQVLTGEGVDVEGEKCKFVRLVDRGGEGSNHWYHVVLTEGK
                     NREVRKMFEAMGLMVSRLMRVRYGPVELPGFLKRGMMRDMPEPDVVKLMEFTGMNEGA
                     GEAAEEEEDEDEASPGNVVTEENRVRVQEDFADEVEDELQPAHLLQDWKPREPAFIDE
                     DDDRQPDFESLPGFNRATGTMGLPAGHRGLHPSRGPGHGTGPGAGPGAGPGAGQKKRR
                     GKRHGPKAGPAGAGQGPFHGKGGGQGQGQGRKRPHGKGHGQGAGKVDGNAQGAAQAMQ
                     GQPQPQGQGQGRGPAQGQGPAAGGPGKPGRNRRHRNRNRNRGASGERPPAANGNGEPS
                     GDS"
     gene            complement(4603..>5077)
                     /locus_tag="VFV55_00210"
     CDS             complement(4603..>5077)
                     /locus_tag="VFV55_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX4942742.1"
                     /translation="RVKPDYQKYLDRISSEKPPRYSRAVLETLAIIAYRQPATRGDIE
                     DIRGVAVSPATLKALEERGWVDVVGQRETPGRPELFATTRKFLDDLNLRSLEELPALE
                     ELQSALDATLDTSPAIPVQEALLGAPEEEADVATPTPEAEANQPSQDTEHPDHAG"
BASE COUNT          708 a         1799 c         1743 g          827 t
ORIGIN      
        1 tcgacgatgg ggcagccggt gcgcgcgagc cagcggtcgg ccacggcgcg ctgcaccgcc
       61 atgccgccgc tgttggccac cctcaggccg gaaaagtcga tgtcgccgaa gcccgggtga
      121 tcgaggagcg cgttgtatag cgtgttcacc gccggaaaga ggtgcgggga gttgcggcgc
      181 aggtcgcgca cgagggcgtc gaggtcgcgc gggttggcaa tgagcaggtt ccgcgcaccc
      241 aggcgcaggc cgaggaagag gcacgccacc agcgcataga tgtgatacag cggcaaggca
      301 cacaccatcg tcgggttttc gacgcggcgc tcgcccgcga aggccggcga aagccacgca
      361 tcggccgcga gcacggcagc caccacattg gcgtgagtga gcatcgcgcc cttcgacacg
      421 cccgtcgtgc cgccggtgta ctgcaggagc gctgtgtccc cgggtcggat cgcgggcgcc
      481 gcaaagggga gcttcgcgcc ccgcgcgaga agttcgtcga agcgcaccgc gccctcgatg
      541 gcccagggcc gcaccgcctt cttcacgtga cgcaggatga agttcgcgac cggccccttc
      601 accggaccca gccagtcgcc cgccgcggcg accgccacgc gcatcggggc cagcccctgc
      661 aacgcctgcg ccagcgtggc cgcgaaattc tcgagcacga cgattgccgc gacttcggca
      721 tccgcgagtt gcgcggcgag ttcccgcgcg gtgaagagcg ggttcacgtt caccaccgtg
      781 gcgccggcac gcagcacgcc gaacaccgcc gccgcgtatt gcgggacgtt gggcatcatg
      841 atcgccactc gcctgccggc gccgatgtcg tgcgaagcga gccaggccgc gaaatgccgc
      901 ccggcgcggt cgacacgtcc gtgggtgaac gaccggccga gaaagacggc ctgttcgcgc
      961 gcgccgtggc gcgcgacggc ctcgtcgatc aggtgcacga gcgtaggggt ggcggagacg
     1021 tccacgtccg caggaacgcc cggcggatag tgggcgagcc aggggcggcc aatgcggtgg
     1081 cagctgctca tttccgcgat aataggggct gcagatatca ggaggacagc cccctgcctg
     1141 ggctcaccat gaacatcgcc gacctgcgca aggactacgc gctcaagcgc ctcgacgaga
     1201 ccgacgtcga cgccgatccc ttcaggcagt tccacgcctg gctcgccgaa gccctcgagg
     1261 cacaggctct cgaacccacg gccatgacgc tcgccaccgc ggatgcgcgc ggccggccgt
     1321 cggcgcgcat catgctgctc aaggccctcg acgatcgcgg cttcgtcttc tacaccaact
     1381 acgcgagccg caagggggaa gaactcgccg cgcggccgca tgccgcgctc accttcttct
     1441 ggaaggaggt ggagcggcag gtgcgcatcg agggcggcat cgagaaggtg agcgccgcgg
     1501 aaagcgacga gtacttcgcc agccggccgc tcggctcgcg catcggcgcc tgggccagca
     1561 cgcaaagcgc cacgatcgaa agccggcaat ggctggaggg acgcgtgaag gccgccgagg
     1621 cgcagtacgg cgacaacccg ccgcgaccgc cgcactgggg cggcttccgg gtgatccccg
     1681 attggctcga gttctggcag gggcgccaga gccgcctgca cgaccgcatc gcctattcgc
     1741 gcgcgggcgg cggctggaag atcacgcgcc tgtcgcccta ggcatgcgaa gtccgctcgg
     1801 ctgccacgac cccgcccggc tggaagcaga aaggctctcc gccgaggcgc tggagcgttc
     1861 cctcgacgag gccctggcgg gccacgaccc gcgcgaaccc ctgtggatct tcgcctacgg
     1921 gtccctgatg tggaacccgg ggctggcgtt cgtcgccaag cgcgtgggca ccatctacgg
     1981 cttccaccgc aatttctgcc tgtggtcgcg tatcaaccgc ggcacgccgg agcacccggg
     2041 gctcgtcctc accctcgagc gcgggggaag ctgccgcggg ctcgccttcc gcctcacggg
     2101 ctccacctcg cgcaacgaat tgctcgcgct ctggcggcgc gaaatgtcga tgggctccta
     2161 ccacccgcgc tggctcgagt tccacgccgg cgcggaccgt cttccggtgc tcgcattcat
     2221 cgtgaaccgc agctgctcgg gctacgcggg aaagcttccg gtggacgcca tggtgcagtc
     2281 gatcgccacg gcacggggca agttcgggtc cagcgcggaa tacctcttcc agacccaggc
     2341 ggcgctcgaa tcgcacggca tcctcgacgt gcaggtgaag cgcctggccg agcgcgtgaa
     2401 ggcctacctc gcgcacggca gcggcggcgc tgccgcccac gcacccgcat agcgccgcgc
     2461 tattcgacga cgagctgtcc ctcgacgccc atttcggcgt ggttgccgag gtggcagaag
     2521 gcgcggtaac ggcccttctc cagcgggatg aaccagagat ccatcgtgcc gccggccgcg
     2581 tacagttcga acttggaaaa gtgtggcgcc ttcagctcgg cgtagcgcgg cacctcggcc
     2641 ttgcgcgccg cgatggtcgc gaagaactcg ggtgcgttga agtagtggtt gttgacgccc
     2701 acgttcacga gcgttagccg gtagggctgg cccaccttga gccgcaactc gcgcggggtg
     2761 aagccggcgt ccttgagctc gatcttcacg tcggtcgtcc gctcccagtc ggccgcggcc
     2821 acgatgcgat cgacttcttc cggcgagcgc gacggctcgg tcgagcaacc tgccacgacg
     2881 caggcgatgc acgtggccac cagggcgtag ggcgacattt cggctctccg gggttcgtca
     2941 ggctttgcgg cagccgcgtc gagccggctg cgattactgc attctaggcc aacgcaaggc
     3001 gctgtccgat gccgtgttca gctatcgccg gatggctcgc cattgccgtt ggcggccggc
     3061 ggccgttcgc ccgatgcgcc gcgattgcgg ttgcgattgc ggtggcggcg gttgcgaccg
     3121 ggcttgcccg gcccgccggc ggcgggtccc tgcccctgcg cgggaccgcg cccctgtccc
     3181 tggccctgcg gctgcggctg accctgcatc gcctgggccg ccccttgcgc gttgccatcg
     3241 accttgcccg caccctgtcc atggcccttg ccgtgcggcc gtttgcgacc ctgcccctgc
     3301 ccttgcccgc cccccttgcc gtggaatggg ccttggcccg ctcccgccgg gcccgccttg
     3361 ggcccgtggc gcttgcctcg ccgcttcttc tggcccgctc ccggtcccgc gcccggtccc
     3421 gcgcccggtc ctgttccatg gccgggtccc cgcgagggat gcagcccgcg atgcccggca
     3481 ggcaggccca tcgtgcccgt cgcccggttg aagcccggca ggctctcgaa gtccggctgc
     3541 cggtcatcgt cctcgtcgat gaacgcgggc tcgcgcggct tccagtcctg cagaaggtgc
     3601 gcgggctgca gctcgtcctc gacttcgtcc gcgaagtcct cctgcaccct gacccggttt
     3661 tcctccgtga ccacgttgcc cggggacgcc tcgtcctcgt cctcttcttc ctccgccgcc
     3721 tcgccggccc cctcgttcat gccggtgaat tccatgagct tcacgacgtc gggctcgggc
     3781 atgtcgcgca tcatgccgcg cttcaggaat ccgggcagtt ccaccggtcc gtagcgcacg
     3841 cgcatgagcc ggctcaccat gaggcccatc gcctcgaaca tcttgcgcac ctcgcggttc
     3901 ttgccctcgg tgaggaccac gtggtaccag tggttggagc cctcgccgcc gcggtccacg
     3961 aggcgcacga acttgcactt ctcgccttcg acgtccacgc cctcgccggt cagcacctgc
     4021 acctgctcgg gcgtgagctc gcccatgagc cgcaccgcgt actcgcgctc gatttcgtag
     4081 cgcgggtgca tcagccggtt ggcgagctcg ccgttggtgg tgaagatgag cagcccgctg
     4141 gtattgaagt cgagccggcc gatggacacc cagcgtgcat tgtttacctt cgggagcttg
     4201 gcgaacaccg tggggcggcc ttccgggtcg tcgcgcgtgc agatctcgcc ttcgggcttg
     4261 tggtacagca gcacgcgcgg cagcaacgag ccgaagcgca ggctgatgag cttcccgtcg
     4321 agcttcacgc ggtcgccggg acccacgaga tcgcccagct tcgccacctg gccgttgatc
     4381 gcgatgtgcc cctgcgcgat gaggatctcg atgttgcgcc gcgagccgtg gcccgactgc
     4441 gccatgacct tctgcaggcg ctcgcgcttg ctggtatccg gtccgccgcg ggggcccttg
     4501 ccgcccttgc cgcggcgcgg cgccgctgcc ggcacttttg cccattcggg cacgatgccc
     4561 ccgggccagt cgagtttggg ggcgcgaggg gtacgaaagg gtctatcccg catggtctgg
     4621 gtgctccgtg tcttgcgagg gctggttcgc ctcggcttcg ggggtgggag ttgcgacgtc
     4681 ggcttcctcc tcaggcgcgc cgaggagcgc ctcctgcacc gggatcgccg gcgaggtgtc
     4741 gagggtcgcg tcgagggcgc tttgcagctc ctcgagtgcg ggcagttcct ccagcgacct
     4801 gaggttcagg tcgtcgagga atttccgggt ggtggcgaag agctccgggc ggccgggggt
     4861 ctcgcgctgg ccgaccacgt cgacccagcc gcgttcctcg agcgccttga gcgtggccgg
     4921 agagacggcg acgccacgga tgtcctcgat gtcgccgcgc gtcgccggct ggcggtacgc
     4981 gatgatggca agcgtttcga gcactgcgcg cgagtagcgc ggcggcttct cgctggagat
     5041 gcggtcgagg tatttctggt agtccggctt cacgcgg
//
LOCUS       DASUEV010000009         9166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1262382, whole genome shotgun sequence.
ACCESSION   DASUEV010000009 DASUEV010000000
VERSION     DASUEV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262382"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFV55_00215"
     CDS             complement(<1..657)
                     /locus_tag="VFV55_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942743.1"
                     /translation="MESSGAQAVQAFVSASGHDGAGMAGFPAGCESDRAVVFPLRLFP
                     GAENSLPSSWSPNPKDRSRDKERTVKPSNEPCKTQRSSLATAVLMALFGMTTGMVQAN
                     FDIPTGSSPSPLFGAQPFTQQMLMFEEFGPQALPTSSAPKTLPAVGGCLGPTQVETTD
                     PNSSWNTSMDAFLTAPLWPAPTEQANTSLPNAWASAISTCLGRQITGPLEGRPPGPNF
                     A"
     gene            1081..2220
                     /locus_tag="VFV55_00220"
     CDS             1081..2220
                     /locus_tag="VFV55_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4942744.1"
                     /translation="MKPTPAIFPGARAWWPAMLLAVTGLAMASPPADLVKGGIDDPKG
                     AGAVSSGVALPPIPPVSGNRNSPVASWGKPLPWPILIADRRNNRLLEVTPDKRIVWEF
                     ASPNLTVYSGNEDVNFSADGKRLAVSEEDNFDVHIVDYDKRELAWTYGVPERKGAKPG
                     YLNYPDDAHLLADGKFLTADIRNCRVVIIDPTTNAIATQWGRQGECRHDPPARLAYPN
                     GATPLDNGDILVSEIRDAWITRITREGKVVWSVKAPNIRYPSDAFPTADGRNVIVADF
                     WKPGRVVIFDPATRRVLWQYDAKSGDGMLDHPSLARELPGTGDIIVADDLRHRVVVID
                     RATKRIIWQYGVTDTPGHEPGYLFYPDGFDLDVFRDWKAAPAASR"
     gene            2322..2957
                     /locus_tag="VFV55_00225"
     CDS             2322..2957
                     /locus_tag="VFV55_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4942745.1"
                     /translation="MAHVEAVPAVEKRARLSSSERRRDVVAAVVALARDRSPTGITTQ
                     AIADRVGLTHGALFRHFPDKQAMWTAVFDWVEERLGAVVDQAFLEGGEPLEILERVFL
                     AHVRFVSGHPGVPRILFHELQGEGESAFRTRVRKAVRGYRARIGWLLAEAKRAGHLPA
                     TLDEEAASVLFVGTVQGLAVQATLFRGEAGMNAAARRLFPLLLDGFRGPRR"
     gene            2954..4138
                     /locus_tag="VFV55_00230"
     CDS             2954..4138
                     /locus_tag="VFV55_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4942746.1"
                     /translation="MKLPAARSLVIGAIIVAVLAGLAWVVTSQGPLARVKVTVTEATA
                     RTLSPSIFGIGTVDARRSYGIGPTAAGRLARVLVDQGAKVVAGQLLAEMDPVDLDERL
                     VAGKAGAQRAEHSARAAQFALDEAQSRARVALSNADRYAELRRTNFVSQEAADAKRHE
                     ANAAQAARDAAEAALAAARDETRRVQADLAGTALSRAHLRLASPVNGVVVARLAEPGS
                     TVVAGQVVVQVIDPASLWVRARIDQGRSAGLAVGLPADVVLRSRPGQPLAGRVERVDL
                     VGDSVTEERLAHVAFEQMPAEATIGDLAEVTLRLPAIEGALAIPTAALKRTGTTRGVW
                     MVNEGRAKFRPIGIGAETLDGFVHVVSGLKAGEAVIVHSGRPLAEGDRVTVTGSLVRG
                     GS"
     gene            4135..5328
                     /locus_tag="VFV55_00235"
     CDS             4135..5328
                     /locus_tag="VFV55_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4942747.1"
                     /translation="MINLAGRDILHGWGKFAFTGVGLGLLIGVTLSMAGIYRGMVEDA
                     TVLIEGSGADLWVVQQGTLGPYAEPSSLRDDIDRTIAGLPGVAETGNIAYLTMQASHG
                     GVESRVMLVGFEPGRPGEPGVLVAGRPVTRDHYEAVADEKAGFRVGDRVRIRRHEYEV
                     VGLTRRMVSASGDPMVFVPLKDAQEVQFLKDNDALLNERARTAANPAFNRPGVPGLLE
                     AVQASQAQSRQVNSVLVRVDAGADPDEVAENIRRWKRLQAYTKAQMEEILVAKLIATS
                     ARQIAMFLVILSVVSAAIVAFIIYTLTVNKVREIAVLKLIGATNATIGGMILQQALGL
                     GVIGFVVGRVVAGFWAPVFPKYVLLVPGDSLRGFAAVMVICALASVVAIRAALRVDPA
                     EAIGG"
     gene            5334..6050
                     /locus_tag="VFV55_00240"
     CDS             5334..6050
                     /locus_tag="VFV55_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4942748.1"
                     /translation="MTDTDTPAIRIQGLRKRYGSGDTAVDALRGVDMTVERGEVVGLI
                     GPSGSGKSTLLKCLGAVIEPTGGRMEISGEAIYDDGWRKADLRALRRDHIGFIFQAPY
                     LISFLDVTDNVALLPMLAGMGNAAARSRARELLAALDVGHRASAQVSQLSGGEQQRVA
                     IARALANHPPVILADEPTAPLDSARALTVVRILNGMAVQFRAAIIVVTHDEKIIPTFR
                     RIYHIRDGRTHEEAGEGRAP"
     gene            6098..6526
                     /gene="tadA"
                     /locus_tag="VFV55_00245"
     CDS             6098..6526
                     /gene="tadA"
                     /locus_tag="VFV55_00245"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX4942749.1"
                     /translation="MREALALAVAAAAQGEVPVGAVVVKEGSIIGRGSNRPIGANDPT
                     AHAEIVALREAAARLGNYRLAGCTLYVTLEPCAMCVGAMLHARLARVVFGAPDPKTGA
                     CGGAVSLAADAKLNHQTTFEGGVLADACGTLLKRFFAERR"
     gene            6586..>9166
                     /gene="dnaE"
                     /locus_tag="VFV55_00250"
     CDS             6586..>9166
                     /gene="dnaE"
                     /locus_tag="VFV55_00250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX4942750.1"
                     /translation="MPAPRFVHLRLHTEYSIVDGMARLDGAVEAAAADGMPALAITDA
                     GNVFGAVKFFLAARASGVQPVIGCDLAISNERNRDQPTRVVVLCRDQEGYRALCDLLT
                     RAYAGNQWRGRAEVRREWLKGVPGLIVLSGAESGDVGAALRAGATAQAEALAREWAGD
                     FPGAFYVELQRADPERSAPYVRAAAALAGRLGLPVVATHPVQFVKAEEFAAHEARVCI
                     AQGWVLGDARRPRDFRPSQYFKTQVEMAELFADLPEALENSVEIARRCSFEFSLGKTR
                     LPDFPTPAGVSVGDYLRALALDGLERHLAEAYPLPAAREAARPRYLERLDYEVATIAQ
                     MGFPGYFLIVADFIGWAKENGVPVGPGRGSGAGSLVAWSLGITGLDPLRYELLFERFL
                     NPERVSMPDFDIDFCQEGRDRVIEYVRAKYGAQSVAQIVTFGTMAAKAVVRDVGRVLG
                     MGYGEVDRIAKLIPFELGITLDKAIEAEPQLKQLIEEQEEVRELMRLARALEGLTRNV
                     GMHAGGVLIAPGKLTDFAPLYCADGSSSTVSQFDKDDVEKAGLVKFDFLGLTTLTIIA
                     ESERHIRAMGQPQFDIQAIPLDDPAAYKVFSSGNTVAIFQFESRGMRDLIMKARPDRL
                     EDLIALNALYRPGPMELIPEFIERKHGRQRWDYPDPRLKEILDPTYGVMTYQEHVMTI
                     AQVIGGYTLGAADLLRRAMGKKKAEEMAQQREIFLKGATERGLDKAKASALFDQMEKF
                     AGYGFNKSHSAAYALVAYQTAYLKAHHAAAFCAANLSAVMNDTDKVQVIFDDARHNGL
                     EVLPPDVNEGGYRFAPLDAKRIRYGLGAVKGTGEQAIDNIVRAREAGGPFRDLGDFCR
                     RVDR"
BASE COUNT         1411 a         3147 c         3229 g         1379 t
ORIGIN      
        1 cgcgaagttg ggtcccggcg gccgcccttc gagcggtccc gtgatctgcc gtcccaagca
       61 ggtgctgatg gcgctcgccc acgcattggg caacgaggtg ttggcctgct cggtgggtgc
      121 cggccaaagc ggcgcggtga ggaacgcatc catcgaggtg ttccagctgc tgttcggatc
      181 cgtggtctca acctgcgtcg ggcccaggca cccgccgacg gccggcaggg tcttgggcgc
      241 ggagctggtc ggcaacgctt gcgggccgaa ctcctcgaac atcagcatct gctgggtgaa
      301 cggctgcgca ccgaacagcg ggctgggcga agagccggtg ggaatgtcga aattcgcctg
      361 gaccatgccg gttgtcatgc cgaacagggc catcaatacg gccgtcgcga gggacgacct
      421 ctgcgtcttg cagggttcgt tgcttggttt cacggtgcgc tccttgtctc ttgacctgtc
      481 tttagggttg ggtgaccacg acgagggcaa ggagttttcg gcgcccggaa acaggcgaag
      541 cgggaacacg acggcgcggt cgctttcgca accagcaggg aaaccagcca tccccgcgcc
      601 gtcgtgcccg cttgctgaaa caaacgcctg tactgcctgt gcgccgctcg attccatttt
      661 tttccctctc ttcgatcagg catcaccgca accgacgact gctgaatgca cgaacggtcg
      721 atgccgacga acggaatgta gtcaatcgga gacatggaaa aaatgtggtt tcttgccgcg
      781 cggcaaattg acgcacgtta ggcgcggctt tgggtcgaaa atcaccgcca ggaaggccga
      841 aatgccgggg aaatgggaag ttcgtcccaa tagcgcgacg tttcccgtta catccgaaac
      901 agaaggcgcg ggcgtcgcgc atcgggaatt tggtatcgac gcgacaccga aggagcgatc
      961 gaagaacggc gcgggagagg gtccatcggc ggccggtccg cgcgcgaagc cgcgagcgca
     1021 agttgcgcaa tccttgcccg cgatcaagcc gcaaccggat tcgggtacta ggctttgcgc
     1081 atgaagccga ctccagccat cttcccaggg gcgcgggcgt ggtggcccgc catgttgctg
     1141 gccgtcacgg ggttggcgat ggcctcgccg ccagccgacc tcgtcaaggg aggcatcgac
     1201 gatccgaagg gggccggcgc cgtctcgagc ggagtggcgc ttccgcccat cccgcccgtc
     1261 agcggcaacc ggaactcgcc ggtcgcctcg tggggcaagc ccctgccgtg gcccatcctc
     1321 atcgccgacc ggcgcaacaa ccgcctcctc gaggtgacgc cggacaagcg catcgtgtgg
     1381 gagttcgcct cgcccaacct gaccgtctac agcggaaacg aggacgtgaa cttctccgcg
     1441 gacggcaagc gcctcgcggt gagcgaggag gacaacttcg acgtccacat cgtcgactac
     1501 gacaagcgcg agctcgcctg gacctacggc gttcccgaac ggaagggcgc gaagccgggc
     1561 tacctcaact atcccgacga cgctcacctg ctcgccgacg gcaagttcct caccgccgac
     1621 atccgcaatt gccgggtggt gatcatcgac ccgacgacca acgccatcgc cacgcagtgg
     1681 ggcaggcagg gcgagtgccg gcacgacccg ccggcgcgcc tcgcctatcc gaacggggcg
     1741 acgcccctcg acaacggcga catcctcgtc tccgagatcc gggacgcgtg gatcacgcgc
     1801 atcacgcgcg agggcaaggt cgtgtggagc gttaaggcgc ccaacatccg ttatccttcc
     1861 gatgcgtttc cgaccgccga cggcaggaac gtcatcgtcg cggatttctg gaagccgggc
     1921 cgcgtggtga tcttcgatcc cgcgacgcgc cgcgtgctgt ggcagtacga cgcgaagtcg
     1981 ggcgacggga tgctcgatca cccctcgctt gcgcgcgaac tgcccggcac gggcgacatc
     2041 atcgtcgcgg acgacctgcg gcaccgcgtt gtcgtgatcg atcgcgccac gaagaggatc
     2101 atctggcaat acggcgtgac cgacacgccg ggacacgaac cgggctatct cttctatccc
     2161 gacggcttcg acctcgacgt cttccgcgac tggaaggcgg cgccggcggc aagccggtag
     2221 cccacccgcc gcgacgggcc ccgggtcgcc aatcgcgcgc ttgcgcgggt cgcgggcctc
     2281 gggtaagcta gtaacaagtt acttactaac ggggctatcc catggcgcat gtcgaggcgg
     2341 tgcccgcggt cgaaaagcgc gcgcggctct cttcctcgga gcgccggcgt gacgtggtgg
     2401 cggcggtggt ggccctggcc cgcgaccgca gcccgacggg catcaccacg caggcgatcg
     2461 ccgaccgggt cggcctgacc catggcgccc tgttccgcca ctttcccgac aagcaggcga
     2521 tgtggaccgc ggtcttcgac tgggtcgagg agcgcctcgg agccgtcgtg gaccaggcgt
     2581 tcctcgaggg cggcgagccc ctcgagatac tcgagcgcgt gtttctcgcc catgtgcgtt
     2641 tcgtctccgg ccatccgggc gtgccgcgca tcctcttcca cgagttgcag ggggagggcg
     2701 aatcggcgtt ccgaacccgg gttcgcaagg cggtgcgcgg ctaccgggcg cgcatcggct
     2761 ggctcctggc cgaggcgaag cgcgcgggcc accttcccgc gacgctcgac gaggaggccg
     2821 cctccgtgct cttcgtgggc accgtgcagg gcctcgcggt gcaagccacg ctcttccgtg
     2881 gcgaagccgg aatgaacgcg gcggcgcggc gcctgtttcc cctcctgctc gacggattcc
     2941 gcgggccgcg ccggtgaagc ttcctgccgc gcgttccctc gtcatcggcg ccatcatcgt
     3001 tgccgtgctg gccgggctcg cctgggttgt cacttcccag gggccgttgg cgcgcgtgaa
     3061 ggtcacggtc acggaggcca ccgcacggac attgtctccc tccatcttcg gcatcgggac
     3121 ggtcgatgcc cgccgctcct acggcatcgg ccccaccgcg gcgggccgcc tggcccgcgt
     3181 gctcgtggac cagggcgcca aggtggtggc gggccagctg ctcgcggaaa tggatcccgt
     3241 ggacctggac gagcgcctgg tcgcgggcaa ggcgggcgcg cagcgcgcgg aacacagcgc
     3301 gcgcgccgcg caattcgcgc tggacgaagc gcagagccgc gcgcgcgtcg cgctgtcgaa
     3361 cgccgaccgg tatgccgaac tgcggcggac gaatttcgtg agccaggagg cggcggatgc
     3421 gaagcggcac gaagcgaacg ccgcccaggc cgcccgcgac gctgccgagg ccgcgctcgc
     3481 cgccgcgcgc gacgagaccc ggcgggtgca ggccgatctt gccggcacgg cgctatcgcg
     3541 cgcccacctt cgcctggcga gcccggtgaa cggcgtggtc gtcgcgcgcc tcgcggagcc
     3601 gggatcgacc gtggtcgcgg ggcaggtcgt cgtgcaggtc atcgatcccg cgagcctctg
     3661 ggtgcgcgcg cgcatcgacc aggggcgatc ggcgggcctc gccgtgggcc tgccggcgga
     3721 cgtggtcctt cgctcccgcc ccgggcagcc tttggccggg cgcgtggaac gcgtggacct
     3781 ggtcggggac tcggtgaccg aggagcgcct cgcgcacgtc gcgttcgagc agatgccggc
     3841 ggaagccacc atcggcgacc tcgccgaagt gacgctgcgc ctgcccgcca ttgaaggggc
     3901 ccttgcgatt ccaaccgcgg cgctgaagcg gaccgggacg acgcgcggcg tctggatggt
     3961 caacgaggga cgcgcgaagt tccggccgat cggcatcggc gccgagacgc tcgacggctt
     4021 cgtccacgtg gtttccggtc tcaaggcggg cgaggccgtg atcgtccata gcggccggcc
     4081 gctggcggag ggcgatcgcg tgacggtgac cgggagcctc gtgcggggcg ggtcatgatc
     4141 aacctggccg gccgcgacat cctgcacggc tggggaaagt tcgcgttcac cggcgtgggc
     4201 cttggcctgc tcatcggcgt gaccctttcc atggccggca tataccgcgg catggtcgag
     4261 gacgcgacgg tgctgatcga gggaagcggc gccgacctgt gggtcgtcca gcagggcacg
     4321 ctcggcccct atgccgaacc ctcgagcctt cgcgacgaca tcgaccgcac gattgccggc
     4381 ctgccgggcg tggcggaaac gggcaacatc gcctacctca ccatgcaggc gagccacggc
     4441 ggcgtggagt cgcgcgtgat gctcgtgggt ttcgagccag ggcgcccggg cgagccgggc
     4501 gtcctcgtcg ccggccggcc cgtcacgcgc gaccactacg aagcggtcgc cgacgagaag
     4561 gccgggttcc gggtcggcga ccgcgtgcgc atccgccgcc acgagtacga ggtggtcggg
     4621 ctcacgcggc gcatggtgtc cgcgagcggc gacccgatgg tcttcgttcc gctcaaggac
     4681 gcgcaggagg tgcagttcct caaggacaac gacgcgctgc tgaacgagcg cgcacgcacg
     4741 gccgccaacc ccgcgttcaa tcgccccggc gtgccgggcc tcctcgaggc cgtgcaggcg
     4801 agccaggccc agagccggca ggtcaacagc gtcctggtgc gggtcgatgc aggcgcagat
     4861 cccgacgagg tggccgagaa catccgtcgc tggaagcggc tgcaggccta cacgaaggcg
     4921 cagatggagg agatcctcgt cgccaagctc atcgccacct cggcccggca gatcgcgatg
     4981 ttcctcgtca tcctttccgt ggtgagcgcg gcgatcgtcg ccttcatcat ctatacgctc
     5041 accgtcaaca aggtgcgcga gatcgcggtg ctgaagctca tcggggcgac caacgccacc
     5101 atcggcggca tgatcctgca gcaggcgctg ggcctgggcg tcatcgggtt cgtggtcggg
     5161 cgagtcgtcg cgggcttctg ggcgccggtg ttcccgaagt acgtgctgct cgttccgggc
     5221 gattccctgc gcggattcgc ggcggtgatg gtgatctgcg cgctcgcgag cgtggtggcg
     5281 atccgggcgg cgttgcgcgt cgatccggcc gaggcgatcg ggggatgacg gcgatgaccg
     5341 acacggacac cccggccatc cgcatccagg gcctgcgcaa gcgctacggc tcgggcgaca
     5401 ccgcggtcga cgcgctccgg ggcgtcgaca tgaccgtgga gcgcggcgag gtggtggggc
     5461 tcatcggccc gagcggctcg ggcaagagca cgctcctcaa gtgcctgggc gcggtgatcg
     5521 agccgaccgg cgggcgcatg gagatttccg gcgaggccat ctacgacgac ggatggcgca
     5581 aggcggacct gcgggcgctg cgccgcgacc acatcggatt catcttccag gcgccgtacc
     5641 tgatctcctt cctcgacgtc accgacaacg tggcgctgct gccgatgctc gcgggcatgg
     5701 gcaacgccgc ggcgcggtcg cgcgcccgcg agctccttgc cgcgctcgac gtcgggcacc
     5761 gcgcctcggc gcaggtgtcg cagctctccg gcggcgagca gcagcgcgtc gcgatcgccc
     5821 gcgccctcgc caaccacccg ccggtgatcc tggccgacga gccgaccgcg ccgctcgaca
     5881 gcgcacgcgc gctgacggtg gtgcggatcc tcaacggcat ggcggtgcag ttccgcgccg
     5941 ccatcatcgt cgtcacccac gacgagaaaa tcattcccac cttcaggcgc atctatcaca
     6001 tccgcgacgg gcggacgcac gaggaggcgg gcgagggacg ggccccgtga aggacgagcc
     6061 gacgctggat ggcattccgg gcctggatga cgcgttcatg cgggaggcgc tcgccctcgc
     6121 ggtcgcggcg gcggcgcagg gcgaggtgcc tgtcggagcc gtcgtggtga aggaaggctc
     6181 gatcatcggc cgcggctcca atcgacccat cggcgcgaac gatcccaccg cgcacgccga
     6241 gatcgtggcc ctgcgcgaag ccgcggcacg gcttggcaac taccggctcg cgggttgcac
     6301 gctatacgtg acgctggaac cctgcgcgat gtgcgttggc gcgatgctgc acgcgcgcct
     6361 ggcgcgcgtc gtgttcggcg cgcccgatcc caagacgggg gcctgcggtg gcgcggtatc
     6421 ccttgcagcg gatgcgaaac tgaaccacca gacgacgttc gaaggcggcg tcctcgccga
     6481 cgcgtgcggc acactcctca agcgcttctt cgccgaacgc cgctagcgcg gccgcgcgtg
     6541 tcgccgggtg tagaatttgc ccgcaatccc acccgtcacg cctcgatgcc cgctccccgt
     6601 ttcgtccacc tgcgcttgca cacggagtat tcgatcgtgg acggcatggc gcgcctggat
     6661 ggtgcggtcg aggcggccgc cgcggacggc atgcccgcgc tcgccatcac ggatgccggc
     6721 aacgtcttcg gggcggtcaa gttcttcctc gccgcgcgcg cgagcggcgt gcagccggtg
     6781 atcggctgcg acctcgcgat cagcaatgaa aggaaccgcg accagcccac gcgagtggtc
     6841 gtgctgtgcc gcgaccagga gggctaccgc gccctctgcg acctcctcac gcgcgcctac
     6901 gccggcaacc agtggcgcgg ccgggccgag gtgcgccgcg agtggctgaa gggcgttccc
     6961 gggctcatcg ttctctcggg ggccgagtcg ggcgacgtcg gcgcggcgct tcgcgcaggc
     7021 gccaccgcgc aggccgaggc cctggcgcgc gaatgggccg gggatttccc cggcgctttc
     7081 tacgtggagc tgcagcgcgc cgacccggag cgcagcgccc cttacgtgcg cgcggcggcg
     7141 gcactggcag gacgcctggg gcttccggtg gtggccacgc accccgtcca gttcgtgaag
     7201 gccgaggaat tcgccgccca cgaggcccgg gtctgcatcg cccagggctg ggtgctgggc
     7261 gacgcgcggc gtccgcgcga cttccggccg tcgcagtact tcaagacgca ggtggagatg
     7321 gcggagctgt tcgccgacct gcccgaggcg ctcgagaact ccgtcgagat cgcgcgccgc
     7381 tgctccttcg agttctcgct gggcaagacg cggctgccgg acttcccgac gcccgcgggt
     7441 gtttcggtcg gcgactacct gcgcgcgctg gcgctcgatg ggctcgaaag acacctggcg
     7501 gaggcgtacc cgctgcccgc ggcgcgcgag gccgcgcggc cgcgctacct cgagcgcctc
     7561 gactacgaag tggccacgat cgcgcagatg ggtttccccg gctacttcct catcgtcgcg
     7621 gacttcatcg gctgggccaa ggaaaacggg gtgcccgtgg ggccggggcg cggctcgggc
     7681 gcgggctcgc tcgtggcgtg gtcgctgggc atcacggggc tcgacccgct gcgctacgag
     7741 ctgctcttcg agcgcttcct caacccggag cgggtgtcga tgccggactt cgacatcgac
     7801 ttctgccagg agggccgcga ccgggtgatc gagtacgtgc gcgccaagta cggcgcgcag
     7861 agcgtcgcgc agatcgtcac cttcggcacc atggcggcca aggcggtcgt gcgcgacgtg
     7921 ggtcgcgtcc tcggcatggg ctacggcgag gtcgaccgca tcgcgaagct gatccccttc
     7981 gagctgggga tcacgctgga caaggccatc gaggcggagc cgcagctcaa gcagctgatc
     8041 gaggagcagg aggaggtgcg cgagctgatg cgccttgccc gggccctcga gggcctcacg
     8101 cgcaacgtgg gcatgcacgc ggggggcgtc ctcatcgccc ccggcaagct caccgacttc
     8161 gcgcccctct actgcgcgga cggctccagt tccacggtga gccagttcga caaggacgac
     8221 gtcgagaagg cgggcctggt gaagttcgac ttcctcggcc tcaccacgct cacgatcatc
     8281 gccgaaagcg agcgccacat ccgcgcgatg gggcagccgc agttcgacat ccaggcgatc
     8341 cccctcgacg accccgcggc ctacaaggtg ttctcctcgg gcaacacggt cgccatcttc
     8401 cagttcgaat cgcgcggcat gcgcgatctc atcatgaagg cgaggcccga ccggctggag
     8461 gacctcatcg ccttgaatgc gctctaccgg ccgggcccga tggaactgat cccggagttc
     8521 atcgaacgca agcacggccg gcagcgctgg gactaccccg acccgcggct gaaggaaatc
     8581 ctcgatccca cctacggcgt catgacctac caggagcacg tcatgacgat cgcgcaggtg
     8641 atcggcggct acacgctcgg agccgcggat ctcctgcggc gcgccatggg caagaagaag
     8701 gccgaggaaa tggcccagca gcgcgagatc ttcctcaagg gcgccaccga gcgcgggctc
     8761 gacaaggcca aggcgagcgc cctcttcgac cagatggaga agttcgcggg ctacggcttc
     8821 aacaagtcgc actccgccgc ctacgcgctg gtggcctacc agacggccta tctcaaggcg
     8881 caccacgcgg cggccttctg cgcggccaac ctgtcggcgg tgatgaacga caccgacaag
     8941 gtgcaggtga tcttcgacga cgcgcgccac aacggcctcg aggtgcttcc gccggacgtg
     9001 aacgagggcg gctaccgctt cgcgccgctg gacgcgaaac gcatccgcta cgggctgggc
     9061 gcggtgaagg gcacgggcga gcaggcgatc gacaacatcg tcagggcgcg cgaggcgggc
     9121 gggcctttcc gggacctggg cgacttctgc cgccgcgtcg accggc
//
LOCUS       DASUEV010000010         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1146618, whole genome shotgun sequence.
ACCESSION   DASUEV010000010 DASUEV010000000
VERSION     DASUEV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146618"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFV55_00255"
     CDS             complement(<1..1187)
                     /locus_tag="VFV55_00255"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX4942751.1"
                     /translation="MKIAVAQINTTVGDFAGNAAGIRAAIDKARAAGAQLVVTPELAL
                     SGYPPEDLLFRNDFCDQCEDELKALASYAPGIAVVVGHPHREGRTRYNAASVLRGGRI
                     EAMYFKQRLPNYNVFDEKRYFATGDRACVFEVGGRRIALTICEDLWFPQSAEQARAAG
                     AELLLSVNASPYHRNKLAQRYQVMGARVKETGLPLLYVHWTGGQDELVFDGASFAFSA
                     DGALGYQGETFLESVDIVEFAGNRLSGPIAPPLTEEETIYRALVTGVRDYVGKNRFPG
                     VLVGLSGGIDSALVAALCADALGPERVHAVMMPSDYTASMSVEDAREMAGLMGLQYSE
                     IAIRPVFDAFRAQLAPAFAGRPEDTTEENLQARTRGTLLMALSNKFGWLVVSTGNKSE
                     MATG"
     gene            1263..2384
                     /locus_tag="VFV55_00260"
     CDS             1263..2384
                     /locus_tag="VFV55_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4942752.1"
                     /translation="MPDSVRLVESLDTIPADAWDRLHDGNPTLAHAFLDSLHRTGCAS
                     PATGWSPQYLTLWEGHALVGAVPLYAKAHSYGEYVFDWAWADAYERHGIAYYPKLLAA
                     VPFTPATGPRLMAASEAIRAGLVQALLDRARREDVSSLHVLFPAAADAAVLRRAGFLE
                     RTGVQFHWRNPGYATFEDFLAAFSHDKRKKIRQERRKVADAGVVLRRVTGREASEADW
                     DFFTACYRRTYREHRSTPYLTREFFAMIAQRMPDSVLLVIAERDGRPIASAFDLFSPA
                     ALYGRYWGAIEYVPGLHFEACYYQAIEFAIERGISLVEGGAQGEHKHARGFLPEKTRS
                     FHWLAHPAFARAIDDYLAREGAGIAAYVDELNERSPFRD"
     gene            complement(2423..3196)
                     /locus_tag="VFV55_00265"
     CDS             complement(2423..3196)
                     /locus_tag="VFV55_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX4942753.1"
                     /translation="MAPTLVFDIETIPDVAALRRVWNLPQDAADEAVVELAHQRRRQA
                     TGSDFLPCHLHRVVAISCALRERDAVRVWTLGSASDGEAELVKRFFDGIDKYTPQLVS
                     WNGSGFDLPVLHYRALFHGIAAPKYWDMGEDDRDFKWNNYISRFHARHIDLMDLLAGY
                     QNRAFAPLDDIAQLCGLPGKLGMDGSKVWQAWRNGEINAIRDYCETDVANTYLVYLRF
                     QRMRGALSAGEYDREIALFRAFLGEQKAAHWREFSSAWK"
     gene            3293..4291
                     /locus_tag="VFV55_00270"
     CDS             3293..4291
                     /locus_tag="VFV55_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX4942754.1"
                     /translation="MSEKNLQVILASRPAGTVVESDFRLAEAPLPEPRDGELLVRVDW
                     LSLDPYMRGRMNEGKSYAARVELGEVMVGGTIGTVVASRHAKYAEGEKVLGMQGWQRF
                     AVSDGQGLVKLPDPRLPPTVWLSATGMPGITAWMGLGLIGTPKAGETVVVSAASGAVG
                     SVAGQLAKIAGCRAVGIAGGAAKCDYVVKELGFDACVDYKAGQLDTALKAAAPSGVDV
                     YFDNVGGEILDAVLRRANVGARVALCGLISGYDGADIPIRNVFSLLVNRVRLQGFIVS
                     DRMDLWPGAMRELAGHVAAGRIKYRETIAQGLESAPRAFIGMLKGENFGKQLVKVS"
BASE COUNT          694 a         1456 c         1501 g          707 t
ORIGIN      
        1 ccggtggcca tttcgctctt gttgccggtg ctcaccacga gccagccgaa cttgttcgac
       61 agggccatga gcagcgtgcc gcgcgtgcgt gcctggaggt tctcctcggt ggtgtcctcg
      121 ggccggccgg cgaacgccgg ggccagctgc gcgcggaagg cgtcgaagac cggccggatc
      181 gcgatctccg agtactgcag ccccatcagc ccggccatct cgcgcgcatc ctcgacgctc
      241 atcgaagccg tgtaatcgga gggcatcatc accgcgtgca cccgctccgg ccccagggca
      301 tcggcacaga gggcggcgac cagcgccgag tcgatgccgc cggagagccc gacgagcaca
      361 cccggaaagc ggttcttgcc gacgtagtcg cgcacgcccg tgaccagcgc gcggtagatg
      421 gtctcctcct cggtgagagg cggagcgata gggccggaga gccggttccc cgcgaactcc
      481 acgatgtcca cggactcgag gaaggtctcg ccctggtagc ccaacgcgcc gtccgcgctg
      541 aacgcgaagg aggcaccgtc gaagacgagc tcgtcctgcc cgcccgtcca gtgcacgtag
      601 agaagcggca gccccgtctc cttcacgcgc gcgcccatga cctggtagcg ctgggccagc
      661 ttgttgcggt ggtagggcga ggcgttgacc gacaggagga gctccgcgcc cgccgccctg
      721 gcctgttccg cggactgcgg gaaccagagg tcctcgcaga tcgtgagcgc gatccggcga
      781 cccccgactt cgaaaacgca ggcacggtcg ccggttgcga aatagcgctt ctcgtcgaag
      841 acgttgtagt tgggcagacg ctgcttgaag tacatcgcct cgatgcggcc cccgcgcagg
      901 acggaggccg cgttgtagcg cgtcctgccc tcgcggtgcg ggtggccgac gaccaccgcg
      961 attcctggag cgtaggacgc cagcgccttc agttcatcct cgcactggtc gcagaagtcg
     1021 ttgcggaaaa ggaggtcctc gggggggtag ccggacagcg ccaactccgg cgtgacgacg
     1081 agctgggcgc cggccgcgcg cgccttgtcg atggccgcgc gaattccggc cgcattgccg
     1141 gcgaagtcgc ccacggtggt gttgatctgg gcgacggcga ttttcatcgg caggaaactt
     1201 cgcgggatgg gccggcgcag tgacggctag aatcggatct cgccattata agtccgccgc
     1261 ccttgcccga ctccgtccgc ctcgtcgagt cgctcgacac cataccggcc gatgcctggg
     1321 accggctgca cgacggcaac ccgacgctcg cccacgcctt cctcgacagc ctgcaccgca
     1381 cgggttgcgc ctcgcccgcc accgggtggt caccgcagta ccttaccctc tgggagggcc
     1441 acgccctcgt cggcgcggta cccctctatg caaaggccca ctcgtatggc gaatacgtgt
     1501 tcgactgggc gtgggccgat gcctacgagc gtcacgggat cgcgtactac ccgaagcttc
     1561 tggcggccgt ccccttcacc cccgcgaccg gtccgcgcct gatggccgcc tccgaggcga
     1621 ttcgcgccgg gctcgtgcag gccctgctcg accgcgcccg gcgggaagat gtctcctcgc
     1681 tgcacgtgct cttcccggcc gcggcggacg ctgccgtcct gcgccgtgcc gggttccttg
     1741 agcgcaccgg cgtgcagttc cactggcgaa accccggcta tgcgacgttc gaggatttcc
     1801 tcgcggcgtt ctcgcacgac aagcgcaaga agatccgcca ggaacggcgc aaggtggccg
     1861 atgccggggt cgtgctcagg cgggtgaccg ggcgcgaggc gagcgaggcc gactgggact
     1921 tcttcacggc ctgctaccgg cgcacctacc gggagcaccg ctccacgccg tacctcacgc
     1981 gcgaattctt cgcgatgatc gcgcagcgca tgcccgacag cgtgctgctg gtcatcgcgg
     2041 aacgcgatgg ccggccgatc gcctctgcgt tcgacctgtt ctctcccgcc gccctctatg
     2101 ggcgttactg gggtgcgatc gagtacgtgc cgggcctgca cttcgaggcc tgctattacc
     2161 aggcgatcga gttcgcgatc gagcgcggaa tcagcctcgt cgagggcggg gcgcagggcg
     2221 agcacaagca cgcgcgcggc ttccttcccg agaagacccg ctccttccac tggctggcgc
     2281 accccgcgtt cgcacgggcc atcgacgact acctcgcccg cgagggcgcg ggcatcgccg
     2341 cctacgtcga cgaactcaac gagcgctcgc cgttccggga ttagggcgga gtctgacacc
     2401 gcacggtgtc tgtcaccgac ccctacttcc aggccgacga aaactcccgc cagtgcgcgg
     2461 ccttctgctc gccgaggaac gcgcggaaca acgcgatctc ccggtcgtac tcgccggcgg
     2521 acagcgcgcc ccgcatgcgc tggaatcgca ggtaaacgag ataggtattg gcgacgtcgg
     2581 tctcgcagta gtcgcggatg gcgttgatct ccccattgcg ccacgcctgc cataccttcg
     2641 agccgtccat gccgagcttg ccgggaaggc cgcagagctg cgcgatgtcg tccagcggcg
     2701 cgaaggcgcg attctggtaa ccggccagga ggtccatgag gtcgatgtgc cgcgcgtgga
     2761 accgcgagat gtagttgttc cacttgaagt cgcggtcgtc ctcgcccatg tcccagtact
     2821 tcggcgcggc aatgccatgg aagagcgcgc gatagtggag cacggggagg tcgaatccgc
     2881 tgccgttcca cgagacgagc tgcggcgtgt acttgtcgat tccgtcgaag aagcgtttca
     2941 cgagctcggc ttcgccgtcg ctggcagagc ccagcgtcca gacgcgcacg gcatcgcgct
     3001 cccgcagcgc gcaggagatc gcgaccacgc ggtgcaggtg gcagggcagg aagtcgctgc
     3061 ccgtggcctg gcgccgccgc tggtgggcga gctccaccac cgcttcgtcg gcggcgtcct
     3121 gcgggagatt ccagacacgg cgcagtgcag ccacgtcggg aatggtttcg atgtcgaaga
     3181 cgagggtcgg tgccatggtc ggggaacgag ccgataacgg gatggagtgc tatcgtcgcg
     3241 aattaccgaa gactagcaac gattgcgcgc cttgcgcaaa gccggaggag ccgtgagcga
     3301 aaagaatctt caggtcatcc tggcgtcccg ccctgccgga acggtggtcg agtcggactt
     3361 tcgcctggcc gaggcgccgc tccccgaacc gcgcgacggc gagcttctcg tccgggtgga
     3421 ctggctgtcg ctcgacccct acatgcgcgg ccgcatgaac gaggggaagt cctacgccgc
     3481 gcgcgtggag ctgggcgaag tgatggtcgg cggcacgatc gggaccgtcg tcgcctcgcg
     3541 gcacgcgaaa tacgccgaag gggaaaaggt cctcggaatg cagggctggc agcgcttcgc
     3601 ggtgtcggac gggcaaggtc tcgtgaaact gcccgacccc cgccttccgc cgacggtctg
     3661 gctcagcgcg accgggatgc ccggcatcac cgcgtggatg ggcctgggtc tcatcggcac
     3721 gccgaaggcc ggcgagacgg ttgtcgtgtc cgcggcgagc ggcgccgtgg gcagcgtggc
     3781 cgggcagctt gcgaagatcg ccgggtgccg cgcggtaggc atcgcgggcg gtgccgcgaa
     3841 atgcgactac gtggtgaagg agctggggtt cgatgcctgc gtggactaca aggcgggcca
     3901 gctcgacacg gcgctcaagg cggcggcccc gtcaggcgtg gacgtctatt tcgacaacgt
     3961 cggcggcgag attctcgacg cggtgctgcg ccgggcgaat gtcggtgcgc gcgtcgcgct
     4021 ctgcggcctc atctcgggct acgacggcgc cgacatcccc atccgcaacg tcttctcgct
     4081 gctcgtgaac cgggtgcggc tgcagggttt catcgtgtcc gaccgcatgg acctctggcc
     4141 cggcgcgatg agggaactgg cgggacacgt ggcggccgga cgcatcaagt accgtgagac
     4201 gatcgcgcag ggactcgaat ccgccccgcg cgccttcatc ggaatgctga agggcgagaa
     4261 tttcggcaag cagctggtga aggtgtcctg atcggaaaag gggtcaggtt actttgaacg
     4321 gaaaaggggt caggttactt ttctgtcgta atgccgtc
//
LOCUS       DASUEV010000011         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_230359, whole genome shotgun sequence.
ACCESSION   DASUEV010000011 DASUEV010000000
VERSION     DASUEV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230359"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1047
                     /locus_tag="VFV55_00275"
     CDS             181..1047
                     /locus_tag="VFV55_00275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX4942755.1"
                     /translation="MIASMTGFAAATSEIAGGQLALELKSVNHRYLEFQCRLADELRS
                     LEPVLREAVAAGLTRGKVDCRLTFTPSAAGERGLAPDDRAMAALAEASARVVSAFPLA
                     RPLTVSDVLHWPGVLANAEVAADQVREDVAKLAAQAVAELSQTRRREGAKLAQVLRER
                     LESMAALGTQAQPLMPEVMRTYRDRLAARLAEASAAPTDERIQQEVVLYAARVDVDEE
                     LERLIAHVAEFRRVLDKGGACGKRLDFLCQELNREANTLGSKSVSSDMTRISVELKVL
                     IEQMREQVQNIE"
     gene            1082..1690
                     /gene="gmk"
                     /locus_tag="VFV55_00280"
     CDS             1082..1690
                     /gene="gmk"
                     /locus_tag="VFV55_00280"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX4942756.1"
                     /translation="MAGDLFVVVAPSGAGKTSLVNAMLDAERAIRLSVSCTTRAPREG
                     EVEGREYHFVSRAAFEEMIAAGDFLEYASVYGNYYGTSRKWIEAELEGEHDVLLEIDW
                     QGARQVRKLFPRLVGIFILPPSIDELRRRLTARGKDSAEVIERRMAGAREEISHVLEF
                     EYIIVNDQFDVALADLQAVVRVARLSRDRRAGRLSRLLDEFR"
     gene            1702..1923
                     /gene="rpoZ"
                     /locus_tag="VFV55_00285"
     CDS             1702..1923
                     /gene="rpoZ"
                     /locus_tag="VFV55_00285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX4942757.1"
                     /translation="MARITVDDCIKYFSNRFELTLAATNRARQLALGASPYVEANRDK
                     PCVVALREIAGGKVGRELLNPPAPTPPVL"
     gene            1932..>2993
                     /locus_tag="VFV55_00290"
     CDS             1932..>2993
                     /locus_tag="VFV55_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX4942758.1"
                     /translation="MPLPGVQAVAGGLKGPGTTPPGPDSSELTAVLGYLPPEDIAQIA
                     RAYETARAAHAGQYRKSGEHYITHPLAVAKILAEWHLDPQALMAALLHDVVEDTPTTK
                     SEIAKQFGKAVAELVDGVSKIDRIEFATLQQAQAENFRKMLLAMARDVRVILIKLADR
                     LHNMRTLDAVHAEKQERIARETHEIYAPIANRLGLNAIYYELEDLGFRYLHPTRFRVL
                     EKALKRARGNRRDAVGKLKDSIQARLREFKVEAQVQGREKHISSIYKKMQEKNISLSE
                     VFDIYGFRILVPDVPSCYLALGALHTLFKPIPGKFKDYIAIPKANGYQSLHTTLFGPF
                     GMPVEVQIRTAEMHMVAEAG"
BASE COUNT          519 a         1034 c          981 g          459 t
ORIGIN      
        1 ggacgtagcc acccggcaac ggctggttca cctgcgaagg catgggatgc ggaggttccg
       61 ctataattcg gtgcattgtc ggcaatcgaa aacggcttgt aaagccgcga gggaaaaggt
      121 ttttttccgc acgaggccgg ccgcttgcct gtttcctacc cgaatcggat cgagctcgcc
      181 atgatcgcca gcatgaccgg ctttgccgcc gccaccagcg aaatcgcggg tgggcagctg
      241 gcattggaac tgaaaagcgt caatcaccgt tacctcgaat tccagtgccg cctggccgac
      301 gaattgcgct cgctcgagcc ggtgcttcgc gaggccgtgg ccgccgggct cacgcgcggc
      361 aaggtcgact gccgcctcac cttcaccccg tccgcggccg gcgagcgtgg cctggcgccg
      421 gacgaccgtg ccatggccgc gctggccgaa gccagcgcgc gcgtcgtgtc ggcgtttccg
      481 ctggcgcggc ccctcaccgt ttccgacgtg ctgcactggc cgggcgttct cgccaatgcg
      541 gaggtcgccg ccgaccaggt ccgcgaggac gtcgcgaagc tcgccgccca ggccgtggcg
      601 gagctttcgc agacgcgccg gcgcgagggc gcgaagctcg cgcaggtcct gcgcgaacgc
      661 ctcgagtcga tggccgccct cggcacgcag gcgcagccgc tgatgccgga ggtgatgagg
      721 acctatcgcg acaggctcgc cgcgcgcctg gccgaggcct ccgcagcccc cacggacgag
      781 cgcatccagc aggaggtcgt tctctacgcg gcgcgcgtcg acgtggacga ggaactcgag
      841 cggctgatcg cccacgtcgc ggagttccgc cgcgtgctcg acaagggggg cgcctgcggc
      901 aagcgcctgg actttctctg ccaggaactc aaccgcgagg ccaacacgct cgggtcgaaa
      961 tccgtctcgt cggacatgac gcgcatctcg gtggagctga aggtgctcat cgagcagatg
     1021 cgcgagcagg tccagaacat cgagtgacgc gattgcgata cacggtcgcg acggtacgct
     1081 gatggccggc gatctcttcg tcgtggtggc gccgtccggg gcgggcaaga ccagcctcgt
     1141 gaacgcgatg ctcgacgccg agcgcgcgat ccgcctgtcc gtctcctgca cgacccgcgc
     1201 gccgcgcgag ggagaggtcg aggggcgcga gtaccacttc gtttcgcgcg cggccttcga
     1261 ggaaatgatc gccgcgggcg atttcctcga gtacgccagc gtgtacggca actactacgg
     1321 aacctcgcgc aagtggatcg aggccgagct cgagggcgag cacgacgtgc tcctcgagat
     1381 cgactggcag ggggcgagac aggtgcgaaa gctctttccg cgcttggtcg ggatcttcat
     1441 cctgcccccc tcgatcgacg agttgcgccg gcgcctgacg gcccgcggca aggactccgc
     1501 cgaggtgatc gagaggcgca tggccggcgc ccgcgaagaa atctcgcatg ttcttgaatt
     1561 tgaatatata attgtcaacg atcaatttga cgtcgcgctc gcggacctgc aggcggtggt
     1621 gcgcgtggcg cgtctttcgc gggaccggcg ggcaggccgc ctctcgcgcc tcctcgacga
     1681 attcaggtaa cggagacaac catggcccgc atcacggtcg acgactgcat caagtacttc
     1741 tccaaccgat tcgagctcac gctcgccgcg accaaccgcg cgcgccagct tgcgctcggc
     1801 gcctcgccct acgtggaagc caacagggac aagccctgcg tggtcgcgct gcgcgagatc
     1861 gcgggcggca aggtaggcag ggagctcctc aatcccccgg ccccgacacc gccggttctc
     1921 tgaactgagt catgcccctc cccggcgtcc aggcggtcgc gggaggcctt aaaggcccgg
     1981 gcacgacccc gcccggcccg gattcgtcgg agcttaccgc cgtcctcggc taccttccgc
     2041 ccgaggacat cgcgcagatc gcaagggcgt acgagacggc gcgcgccgcg cacgccggcc
     2101 agtaccgcaa gagcggcgag cactacatca cccacccgct cgcggtggcg aagatcctcg
     2161 ccgagtggca cctcgacccg caggcgctca tggcggcgct gctgcacgac gtggtggagg
     2221 acacgcccac caccaagtcg gagatcgcga agcagttcgg caaggccgtg gcggagctcg
     2281 tcgacggcgt ctcgaagatc gaccgcatcg agttcgccac cctgcagcag gcgcaggccg
     2341 agaacttccg gaagatgctc ctcgcgatgg cgcgcgacgt gcgcgtcatc ctcatcaagc
     2401 tggccgaccg cctgcacaac atgcgcacgc tggacgccgt gcacgcggaa aagcaggagc
     2461 gcatcgcgcg cgagacgcac gagatctacg cgcccatcgc caaccgcctg gggctcaacg
     2521 ccatctacta cgagctcgag gacctcggct tccgttacct tcatccgacg cgctttcgcg
     2581 tgctcgagaa ggcgctcaag cgcgcgcgcg gcaaccggcg cgacgccgtg ggcaagctga
     2641 aggactcgat ccaggcgcgc ctcagggaat tcaaggtcga ggcgcaggtg cagggccgcg
     2701 agaagcacat ctcctccatc tacaagaaga tgcaggagaa gaacatctcg ttgtccgagg
     2761 tgttcgacat ctacggcttc cgcatcctcg tgcccgacgt gccgtcgtgc tacctcgccc
     2821 tgggcgcgct gcacacgctc ttcaagccca tccccggcaa gttcaaggac tacatcgcca
     2881 tccccaaggc gaacggctac cagtcgctgc acaccacgct cttcggcccg ttcgggatgc
     2941 ccgtcgaggt gcagatccgc accgccgaga tgcacatggt ggccgaggcc ggc
//
LOCUS       DASUEV010000012         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_2910, whole genome shotgun sequence.
ACCESSION   DASUEV010000012 DASUEV010000000
VERSION     DASUEV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1174)
                     /locus_tag="VFV55_00295"
     CDS             complement(29..1174)
                     /locus_tag="VFV55_00295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4942759.1"
                     /translation="MLRAQFLVLALLSVHVLLGMLCLATSRGERDSPALRQWGWGLVV
                     YAAGLAITIMAFLPRPVGNFAGNSLIGLSSALAVQGVLYYTDRRLNRPAALGGIAATI
                     AVLAVGNFAMSPSMAVNFVAPTVIATAFFTYGAAMLLLHPRPDAVRPARFVALMLVAA
                     ILVWWIRLFAMPPLLAEPVDRERLDMVVSSFAIAQILVGVGATFGLFWVEVLQVQATI
                     ARMAFNDSLTGLANRRAIVARFEEEAARSSRTGVGFALAVFDIDRFKDVNDTHGHLAG
                     DALLCHVGRLLAGAKRTEDVLGRLGGEEFVVLMCGHQSGGAAIAADRLRQLVAATPLA
                     FDGKELSVTLSGGLARLPEDGNDWDKVFAAADARLYEAKNAGRNRVV"
     gene            complement(1203..1550)
                     /locus_tag="VFV55_00300"
     CDS             complement(1203..1550)
                     /locus_tag="VFV55_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4942760.1"
                     /translation="MTIQRFHVAKRLSEMVVHDGTIYLAGQVAADMTRDIIGQTEQVL
                     ASIDRLLGEAGSDKTKILSAQIFLPDMGDFAAMNSVWERWVAQGHPPARATLEAKLAN
                     PAYRIEVVIVAAL"
BASE COUNT          361 a          677 c          671 g          319 t
ORIGIN      
        1 attttttgtg caaagtaacc tgaccccttt acaccaccct gttgcggccg gcattcttgg
       61 cctcgtagag gcgagcgtcc gcggcggcga agactttgtc ccaatcgttg ccgtcctcgg
      121 gcagccgggc caggccgccg ctcagggtga ccgagagttc cttgccgtcg aatgccagcg
      181 gcgtggccgc caccagctgg cgcagccggt cggccgcgat ggccgctccc ccggactggt
      241 gtccgcacat cagcacgacg aattcctcgc cgccgagccg gcccagcacg tcctcggtgc
      301 gcttggcgcc ggcgagcagc cggccaacgt ggcaaagcag cgcatcgccc gcgaggtggc
      361 catgggtgtc gttcacatcc ttgaagcgat cgatgtcgaa gacggcgagc gcgaacccga
      421 ccccggtgcg cgacgagcgc gccgcttcct cctcgaaacg ggcgacgatg gcgcgccggt
      481 tcgccagccc ggtgagcgaa tcgttgaacg ccatgcgcgc gatcgtcgcc tgcacctgca
      541 gcacctccac ccagaacagg ccgaaggtcg cgcccacgcc caccaggatc tgggcgatgg
      601 cgaaggacga gacgaccatg tccagccgct cgcggtcgac gggctcggcc agcagcgggg
      661 gcatggcaaa gaggcgtatc caccacacaa ggatcgccgc gacgagcatc agggcgacga
      721 agcgggccgg acgcacggcg tccggccgcg gatgcaggag cagcatcgcc gcgccgtagg
      781 tgaagaatgc cgtggcgatc accgtcggcg ccacgaagtt caccgccatg ctcggggaca
      841 tcgcgaagtt gcccacggcc agcaccgcga tggtcgccgc gatgccgccg agcgcggccg
      901 gccggttcag gcgccggtcc gtgtaataca gcacgccctg gacggcgagc gcggaggaca
      961 ggccgatgag cgaattgccc gcgaagttgc cgacgggcct cggcaggaag gccatgatcg
     1021 tgattgcgag ccctgccgcg tacacgacga ggccccatcc ccactggcga agcgcaggcg
     1081 aatcccgttc cccgcgcgag gtcgcgaggc acagcatccc caggagcacg tgcacgctga
     1141 gcagggcgag gacgaggaat tgcgcgcgga gcatgaactg ctgtcggccg gccggggcgc
     1201 gctcagagcg ccgcgacgat caccacctcg atcctgtacg cggggttggc gagcttcgct
     1261 tccagcgtcg cacgcgcggg cggatgcccc tgcgccaccc agcgctccca caccgaattc
     1321 atcgccgcga agtcgcccat gtccggaagg aagatctgcg cggagaggat cttcgtcttg
     1381 tcgctgcccg cctcgcccag caaccggtcg atggaggcga gcacctgctc cgtctggccg
     1441 atgatgtccc gggtcatgtc cgcggccacc tgcccggcga ggtagatcgt gccgtcgtgc
     1501 acgaccatct cggaaaggcg cttggccacg tggaatcgct ggatcgtcat gttttctccc
     1561 tgttgcgtcg tcgtgaagtc ggtcgttcgt cgaagaggcg ccggtgacgc gcgacctcga
     1621 tatcgtcatg ccgccattgt ccgtgatttc gaatgcgcgg gcaggactgt cgcatgcggt
     1681 gatgcccgca gagtcgaatg ggaccgtcgt atgcggtgat gcccgcagat tcgaatggga
     1741 ccgtcgtatg cggtaatgcc cgcagattcg aatgggaccg tcgtatgcgg taatgcccgc
     1801 agattcgaac agggaccagg tgtggttggg tttttttcgg attttggccg gatgaattgg
     1861 ccgcgagctt cgactccgga aactcttcga catcgtttcg aaccgtgacg cccgacgcaa
     1921 tctaacccgg ccaaaatccg aaaaacaacc caaccacacc tggtcccggt ggtgcgcgac
     1981 ggcccgacct ccaaattgag gggaatgccc attgcgacgg cccaaccg
//
LOCUS       DASUEV010000013         6552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_461251, whole genome shotgun sequence.
ACCESSION   DASUEV010000013 DASUEV010000000
VERSION     DASUEV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461251"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2138)
                     /locus_tag="VFV55_00305"
     CDS             complement(105..2138)
                     /locus_tag="VFV55_00305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX4942761.1"
                     /translation="MENPLLDFSGLPRFAAIRPEHVVPAIDELVAEGRAAIERLGSAG
                     GEPTWEGFVEPLESANERLARAWAQVCHLNAVVNSPVLREAYNAALPRVTQFHAEQGQ
                     DPRLFGRFKALAASPEFARLSPAKRRLVDNELRDFRLGGAELPEAGKARFRQLKEELA
                     ALSARFQDNVLDATNGFGLMVTDPAHLSGIPADVLEVAKGAAAKDGREGWKLTLHMPC
                     YLPVMQYADWRPLREQMYRAFVTRASEFGKPEWDNTPLIAKILERRAEVAKLLGFDTF
                     AGVSLATKMAKTPSEVITFLEDLAARAKPFAERDVAEAAEFARTHLGLAEVQAWDVPW
                     VSEKLRQGRYAFSDEEVKRYFPEDAVLAGLFRVIEALYGVRVKPSRTESWHPDVKFFA
                     IEDAKGDLVGQFYLDLYAREAKRGGAWMDDARNRRRIASGLQTPVTFLTCNFSAPVGG
                     RPAFFTHNEVITLFHECGHGLHQLLTRVDELGVSGLAGVEWDAVELPSQFMENFCWEW
                     EVVSHMTRHGDTGERLPRAIFDRMVAAKNFQSGMQFVRQIEFALFDMHLHHDFDASRD
                     DVLAMLGRVRRQVAVIQPPEWNRFPSQFSHIFGGGYAAGYYSYKWAEVLSADAFGAFE
                     EEGVLNPATGERFRNEVLGVGGSRPAIESFVAFRGRKPRIDALLRHNGMNATA"
     gene            complement(2173..3447)
                     /locus_tag="VFV55_00310"
     CDS             complement(2173..3447)
                     /locus_tag="VFV55_00310"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX4942762.1"
                     /translation="MKIDIRNARIIDPATGRDEIAPLFIADGRIVALGTAPEGFRPDK
                     TIDAKGLVACPGLVDLSARLREPGFEYRATLESEMQAAVAGGVTSLACPPDTDPPLDE
                     PGLVEMLKRRAESLRQARVYPLGALTLGLRGEKIAEMGELAEAGCVGFFQADAPLTDL
                     NVLLQAMRYAATFGHAVWLRPQDSHLAHGGVAHEGEVSTRLGLPPIPEVAETVALHAI
                     LDLAEATGARVHLARLSTARGVALVAAAKAHGLAVTCDVGVHHLHLCDRDAGDFNANM
                     NLVPPLRDPRDRDALRRALADGTIDAVCSDHTPVDDDAKQVPFGEAEPGATGLELLLP
                     LVLKWGAEMKLPLAATLAAVTSRPAAILGIESGTLATGRAADLCLFDPERHWIVSPSA
                     LASQGRNSPFLGLEVVGRVTTTIVDGRVVYAG"
     gene            complement(3449..4402)
                     /locus_tag="VFV55_00315"
     CDS             complement(3449..4402)
                     /locus_tag="VFV55_00315"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX4942763.1"
                     /translation="MNRSLQVDSQGRLVHLLTTEGLPAEMIRHILDTASQFVSVNERE
                     VKKVPLLRGKSIFNVFFENSTRTRTTFEIAAKRLSADVINLNIGASSTAKGETLLDTV
                     WNLQAMDADMFVVRHAEAGAPHLIARHVKPGVHVVNAGDGRHAHPTQGLLDMYTIRHY
                     KQDFTRLVVAIVGDVLHSRVARSEIHALKTLGVPDVRVIGPKTLLPVDIERLGVRVFH
                     DLAEGLRDCDVVITLRLQNERMSGPLLPSAGEFHKAYGLTPERLARARPDAIVMHPGP
                     LNRGVEIDSGVADGSHSVIMPQVTFGIAVRMAVMAILAGNA"
     gene            complement(4399..4902)
                     /gene="pyrR"
                     /locus_tag="VFV55_00320"
     CDS             complement(4399..4902)
                     /gene="pyrR"
                     /locus_tag="VFV55_00320"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX4942764.1"
                     /translation="MAAPLPEPERLLEALVTWMKPSTGPETGIVGIYTGGAWVAERLH
                     RLLGVKTPLGVLAVTLHRDDFGRIGLHREARRSHLPFEVEGRDIVLVDDVLHTGRTIR
                     AALNELWDFGRPKSVRLAILADRGGRELPIAPDFTGAFVEMQPDEELVLTNEGGKLRL
                     AAVRRPG"
     gene            complement(4883..5422)
                     /gene="ruvX"
                     /locus_tag="VFV55_00325"
     CDS             complement(4883..5422)
                     /gene="ruvX"
                     /locus_tag="VFV55_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX4942765.1"
                     /translation="MSQKGDASLFSEIEENQMRGKGKSHLPAPLSGTLLGFDFGEKRL
                     GVAVGETQTRLAHPVAAIAEEATEARFAAIGRLVAEWHPAGFVVGLPRHADGSEHAVA
                     RLANKFARRLSARFGLPVAFVDETLTSAEAAMLLREAGGRAKGKGDLDAQAAALILQS
                     YLDDMNRPIAKGTHGRTPA"
     gene            complement(5422..5979)
                     /locus_tag="VFV55_00330"
     CDS             complement(5422..5979)
                     /locus_tag="VFV55_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX4942766.1"
                     /translation="MHTINLTSHFLIAMPGMVDPNFSRSLTFICEHNERGALGLVVNR
                     PTDVTLGTLFRQVEIDLEDEPLASQLVFFGGPVQLEHGFVLHRPLGDWRSTLPVGEIG
                     LTTSRDILEAMARGDGPSDRLVALGYAGWAPGQLEEEIKRNGWLTVNADLDVIFRVPP
                     EGRYDAALGALGVSTMNLSEEAGHA"
     gene            6060..>6552
                     /locus_tag="VFV55_00335"
     CDS             6060..>6552
                     /locus_tag="VFV55_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX4942767.1"
                     /translation="MTAYDRSLFWFRRDLRDEDNAGLHFALVSSRQVHCVFVFDREIL
                     DALPSRADRRVEFIWESVAELRKALQGRGGGLIVLHGRAREEIPALAARLGAAAVFAN
                     EDYEPAALARDTSVEKALAADGRRLHLAKDQVVFAKDEILTRSGGRYTVFTPYKRAWL
                     AKLD"
BASE COUNT         1039 a         2206 c         2283 g         1024 t
ORIGIN      
        1 gacccagcga tagacgttgg cctgcgccca ggccagcgcc ggcagcagca gcgccgcgac
       61 gaacaacgcg ggcagtcggg aacgggcagg catcgattcc tccgtcaggc ggtggcgttc
      121 atcccattat gccgcaggag cgcgtcgatc cggggcttgc ggccgcggaa ggcgacgaac
      181 gactcgatgg ccggacggct gccgccgacg cccagcacct cgttgcggaa gcgctcgccg
      241 gtcgcggggt tcaggacgcc ttcctcctcg aaggcgccga aggcgtccgc cgagagcact
      301 tccgcccact tgtagctgta gtaaccggcc gcatagccgc cgccgaagat gtgcgagaac
      361 tggctgggga accgattcca ctccgggggc tggatgacgg ccacctggcg ccgcacgcgc
      421 ccgagcatcg cgagcacgtc gtcgcgcgag gcgtcgaaat cgtgatgcag gtgcatgtcg
      481 aagagcgcga actcgatctg gcgcacgaac tgcatgccgc tctggaaatt cttggccgcg
      541 accatcctgt cgaagatcgc gcgcgggagc cgctccccgg tgtcgccgtg ccgcgtcatg
      601 tgcgagacga cttcccattc ccagcagaaa ttctccatga actgggacgg aagctcgacc
      661 gcgtcccatt ccacgccggc gagacccgaa accccgagct cgtccacgcg cgtcaggagc
      721 tggtgcaggc cgtggccgca ctcgtggaag agcgtgatca cctcgttgtg ggtgaagaac
      781 gcggggcgtc cgcctacggg agccgagaag ttgcaggtga ggaaggtaac gggcgtctgc
      841 aggcccgagg caatgcgccg gcggttcctc gcgtcgtcca tccacgcgcc gccgcgtttt
      901 gcctcgcggg cgtagaggtc gagatagaac tggcccacca ggtcgccctt cgcgtcctcg
      961 atggcgaaga acttcacgtc cgggtgccag ctctcggtcc gcgaaggctt cacgcgcacg
     1021 ccgtagagcg cctcgatcac gcggaacaag ccggcgagca ccgcgtcctc ggggaaatag
     1081 cgcttcacct cctcgtcgga gaacgcgtag cgcccctggc gcagcttctc gctcacccac
     1141 ggcacgtccc aggcctgtac ttccgcgagg ccgaggtgcg tccgcgcgaa ctccgccgct
     1201 tccgcgacgt cgcgttccgc gaacggcttc gcccgtgcgg ccaggtcctc gaggaaggtg
     1261 atgacttccg acggggtctt cgccatcttg gtggcgagcg acacgcctgc gaaggtgtcg
     1321 aagccgagga gcttcgcgac ttccgcgcgc ctctccagga tcttcgcgat gaggggcgtg
     1381 ttgtcccact cgggcttgcc gaactcggag gcgcgcgtca cgaaggcgcg gtacatctgc
     1441 tcgcgcagcg ggcgccaatc cgcgtactgc atgaccggca ggtagcaggg catgtgcaac
     1501 gtgagtttcc agccctcgcg cccgtccttc gcggccgcgc ccttcgcgac ctccaggaca
     1561 tccgcgggaa tgccggaaag atgcgccggg tcggtgacca tgaggccgaa gccgttggtg
     1621 gcgtcgagca cattgtcctg gaatcgcgcc gaaagcgcgg cgagctcctc cttgagctgc
     1681 cggaagcgcg ccttgccggc ttcggggagc tccgcgccgc caaggcggaa gtcgcgcagc
     1741 tcgttgtcca cgagccggcg cttcgcgggc gaaagacggg cgaactccgg ggacgcggcg
     1801 agcgccttga agcggccgaa aagacgcggg tcctggcctt gctcggcatg gaactgcgtc
     1861 accctgggca gcgccgcgtt gtaggcctcg cgaagcaccg gcgagttgac gacggcattg
     1921 aggtggcaga cctgggccca cgcgcgcgcc aggcgctcgt tcgcgctttc gaggggctcg
     1981 acgaaacctt cccaggtcgg ctcgccgccc gcgctgccga gccgctcgat cgcggcgcgg
     2041 ccctcggcaa ccagttcgtc gatggcgggc accacgtgtt cgggccgtat cgcggcaaag
     2101 cgcggaaggc cggaaaagtc cagcagcggg ttctccatgg gaatccgttc ctgaggaaga
     2161 atgggcaagg gatcagccgg cgtacacgac gcggccatcg acaatcgtcg tcgtgacgcg
     2221 ccccacgact tccagtccca ggaatgggga gttgcgaccc tggctcgcaa gggcagacgg
     2281 cgaaacgatc cagtgacgct cggggtcgaa aagacagagg tcggcggcac ggcccgtcgc
     2341 cagcgtgccg gactcgatac cgagaatggc cgcggggcgc gaggtcacgg cggccagcgt
     2401 ggcggcaagc ggcagtttca tctccgcgcc ccacttgagg accagcggaa ggagaagttc
     2461 gagcccggtg gcccccggtt cggcctcgcc gaagggtacc tgtttcgcgt cgtcgtccac
     2521 cggcgtgtgg tcggagcaca ccgcgtcgat ggtcccgtcg gcgagcgcgc gccgaagggc
     2581 gtcgcggtcg cggggatcgc gaagcggcgg cacgagattc atgttggcgt tgaagtcgcc
     2641 ggcatcgcgg tcgcagaggt gcaggtggtg cacgcccacg tcgcaggtta cggcaagccc
     2701 gtgcgccttg gcggcggcca cgagggccac gccccgtgcc gtggagagcc gcgcgaggtg
     2761 aacccgtgcg ccggtggctt cggcaagatc gagaatcgcg tgcagggcaa ccgtttcggc
     2821 cacctcgggg atgggcggaa gtcccaggcg cgtgctcacc tcgccctcat gcgcgacccc
     2881 gccgtgggcg aggtgggaat cctgcggacg cagccagacc gcgtggccga atgtcgccgc
     2941 atagcgcatc gcctgcagca acacgttcag gtcggtaagc ggcgcgtcgg cctggaagaa
     3001 tccgacgcat ccggcttccg cgagctcgcc catctcggcg atcttctcgc cccgcaatcc
     3061 gagcgtcagt gcgcccagcg gatagacgcg cgcctggcga aggctctcgg cgcggcgctt
     3121 gagcatctcg accagccccg gctcgtcgag tggcgggtcc gtgtccggcg ggcaggcgag
     3181 cgaagtcacg ccgccggcga ccgccgcctg catctcggat tccagggtgg cgcggtactc
     3241 gaagcccggc tcgcgaaggc gcgcggaaag gtccacgagc ccgggacagg cgacgaggcc
     3301 cttcgcgtcg atggtcttgt cgggccggaa accctcggga gccgttccca gggccacgat
     3361 gcgcccgtcg gcaatgaaga gaggggcaat ctcgtcgcgg cccgtggccg ggtcgatgat
     3421 gcgggcattg cggatgtcga ttttcatgtc acgcgttccc ggcgagaatc gccatcacgg
     3481 ccatgcgcac cgcgatgccg aaggtgacct gcggcatgat cacgctgtgg ctgccgtcgg
     3541 ccacgcccga gtcgatctcc acgccgcggt tgagagggcc cggatgcatc acgatggcat
     3601 cgggcctggc acgcgcgagc ctttccggcg taaggccata ggccttgtgg aactccccgg
     3661 ccgagggcag cagcgggccg ctcatgcgct cgttctgcag gcgcagggtg atcaccacgt
     3721 cgcagtcgcg aagtccttcc gccaggtcgt ggaagacacg cacgccgagg cgctcgatgt
     3781 cgacgggaag cagcgtcttc ggcccgatca cgcgcacatc gggaacgccg agggtcttca
     3841 gtgcgtggat ctcggatcgc gccacgcgcg agtgcaggac atcgccaacg atcgccacga
     3901 cgagccgcgt gaagtcctgc ttgtagtggc ggatcgtgta catgtcgagc agcccctgcg
     3961 tggggtgcgc gtggcgcccg tcgcccgcgt tgaccacgtg cacgccgggc ttcacgtggc
     4021 gcgcgatgag gtgcggcgcg ccggcttccg cgtggcggac cacgaacatg tcggcgtcca
     4081 tcgcctgcag gttccacacc gtgtcgagga gcgtctcgcc ctttgccgtg gaggaagccc
     4141 cgatattcag gttgatcacg tcggccgaaa ggcgcttcgc ggcgatctcg aaggtggtgc
     4201 gggtgcgcgt ggaattctcg aagaagacgt tgaagatcga cttgccgcgc agcagcggca
     4261 ccttcttcac ctcgcgctcg ttcacgctca cgaactgcga agcggtgtcg aggatgtgcc
     4321 ggatcatttc cgccggcagg ccctcggtgg tgaggaggtg aacgaggcgt ccctgcgaat
     4381 cgacctggag gctgcgattc atcccggcct ccgcacggcg gcaaggcgca gcttgccccc
     4441 ttcgttggtc agcaccagct cttcgtcggg ctgcatctcc acgaaggcgc cggtgaaatc
     4501 cggcgcgatg ggaagctcgc gccccccgcg atccgcgagg atcgccaggc gcacgctttt
     4561 cggccggccg aagtcccaca gttcgttcag ggccgcgcgg atggtgcgac cggtgtgcag
     4621 gacgtcgtcc acgagcacga tgtcgcgccc ctcgacctcg aacgggaggt ggctgcggcg
     4681 cgcctcgcgg tgcaggccta tgcggccgaa gtcgtcgcgg tgcagcgtta cggcaaggac
     4741 accgagcggc gtcttcacgc cgagcaggcg gtgcaggcgt tccgccaccc acgcgcctcc
     4801 ggtgtagatg ccgacgattc ccgtctcggg gcccgtcgag ggcttcatcc aggtcacgag
     4861 cgcctcgagc aggcgctccg gttcaggcag gggtgcggcc atgggttccc ttcgcaattg
     4921 gtcggttcat gtcgtccagg tagctctgca ggatcagggc ggcggcctgg gcgtccaggt
     4981 cgcccttgcc cttcgcgcgg ccgcccgcct cgcgcaggag catcgcggct tcggcggagg
     5041 tgagggtttc gtccacgaag gcgacgggca acccgaagcg ggcgctgagg cgccgggcaa
     5101 acttgttcgc gagcctcgcc accgcgtgct cgctgccgtc cgcgtggcgc ggcaatccga
     5161 ccacgaaacc ggccggatgc cattcggcca ccagccgccc gatggcggcg aatcgggcct
     5221 cggtggcctc ctccgcgatg gcggcgacgg ggtgggcaag ccgtgtctgc gtctctccaa
     5281 cggccacgcc caggcgcttc tcgccgaagt cgaagccgag cagggtcccg gaaagcggcg
     5341 caggaaggtg actcttccct tttccccgca tctggttttc ctcgatttcg gagaaaaggg
     5401 aagcgtcgcc tttctgcgac atcacgcgtg ccccgcctct tccgagaggt tcatggtcga
     5461 gacgccaagc gcgccaagcg ccgcgtcgta acggccctcc ggcgggacgc ggaagatcac
     5521 gtcgaggtcc gcgttgaccg tgagccagcc gttgcgcttg atctcttcct cgagctgtcc
     5581 cggcgcccag ccggcgtagc ccagggccac cagccggtcg ctggggccgt cgccgcgcgc
     5641 catggcctcg aggatgtcgc gggaggttgt gagcccgatc tcgcccaccg gcagcgtcga
     5701 gcgccagtcg cccagcggcc ggtgcaggac gaacccgtgc tcgagctgga cgggcccgcc
     5761 gaaaaacacc agctgcgagg cgaggggctc gtcctcgagg tcgatctcca cctggcggaa
     5821 cagggtaccc agcgtcacgt ccgtcgggcg attgaccacc agcccgaggg cgccacgctc
     5881 gttgtgctcg caaatgaagg tgaggctcct ggaaaaattc gggtccacca tgccgggcat
     5941 ggcgatcagg aagtggctcg tgaggtttat cgtatgcatt tgccaccgac cccacattct
     6001 aattcgaacg cctcgcggcc gccatcggac ccgggacgaa gggcccacgc aagccgcccg
     6061 tgaccgccta cgaccgatcc ctcttctggt tccggcgcga cctgcgcgac gaggacaacg
     6121 ccgggctgca tttcgcgctc gtgtcgtccc ggcaggttca ctgcgtcttc gttttcgacc
     6181 gcgaaatcct ggatgcgttg ccttcccgag ccgaccggcg cgtcgagttc atttgggaaa
     6241 gcgtcgcgga attgcgcaag gccctccagg ggaggggtgg tggcctcatc gtcctgcacg
     6301 gccgggcgcg cgaggagatc ccggcgctcg cggcgcggct gggcgcggcg gccgtcttcg
     6361 ccaacgagga ctacgagccc gccgcccttg cgcgcgacac cagtgttgaa aaggcactcg
     6421 cggcggacgg gcgccgcctg catctcgcca aggaccaggt ggtcttcgcg aaggacgaga
     6481 tcctgacgcg gtccgggggc cgatataccg tgttcacgcc ttacaagcgc gcctggctcg
     6541 ccaagctgga cg
//
LOCUS       DASUEV010000014         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_690415, whole genome shotgun sequence.
ACCESSION   DASUEV010000014 DASUEV010000000
VERSION     DASUEV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690415"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFV55_00340"
     CDS             <1..438
                     /locus_tag="VFV55_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX4942768.1"
                     /translation="EALSRAGASGLMQLMPATARWVAKQIPVASYRPALLAQPEVNVA
                     MGTYYFHRVLVDVGDPVLATAGYNAGPGRAKRWRDAKPLEGAIYAETIPFNETRDYVK
                     KVMVNAWFYTHRLTGRLASLHRLMGTVPGRGPGEAANSVAAIP"
     gene            438..1385
                     /locus_tag="VFV55_00345"
     CDS             438..1385
                     /locus_tag="VFV55_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX4942769.1"
                     /translation="MDNARVLVLGATGFVGRSVCARLEARGTGLRVLTRDLLKARPLQ
                     VIPSIEIVTGSPHDDADLARALDGVDAAVNLVGILHQSRREPFEKVHVELPRRLARAC
                     RAAGVRRLVHMSALHAGRDGPSEYLRSRGRGEDAIREEGAGLAVTIFRPSVIFGQDDS
                     FLNLFAGLVRFLPVVPLAGAGTKFQPVWVEDVARTVAACLDDPRTSGKTYELCGPGTY
                     ALAQIVDFVAGLQGRKRLVVPLPGWAAQAQAYVLEHLPGPVMTRDNLASMKVDSVCGC
                     PFPEELGFAPATMEAIVPEYMAAAGLRGRYADYRRRGGR"
     gene            1387..>2057
                     /locus_tag="VFV55_00350"
     CDS             1387..>2057
                     /locus_tag="VFV55_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4942770.1"
                     /translation="MADLTLVIGNKNYSSWSMRPWVLLTQLGIPFEERKLRFNSDEWD
                     AGIGRWSPSGLVPVLWRGNEAVWDSLAIMEAVAEWFPDKGVWPADPAARALARSTAAE
                     MHAGFRNLRAKMPMSIRSRYPGKGMTPEVRADIDRIEHLWRDARSRFGSGGDFLFGRF
                     SAADAMFAPVVMRFATYGVTCGPVSARYCRAMQAAAGVRAWIEGAQQETEFVAEDEPY
                     AEPPRP"
BASE COUNT          290 a          720 c          736 g          311 t
ORIGIN      
        1 gaggccctct cgcgcgcggg cgccagcggc ctcatgcagc tcatgccggc gacggcgcga
       61 tgggtggcca agcagattcc ggtcgcctcg taccggcccg cgctcctcgc gcagccggag
      121 gtgaatgtcg cgatgggcac ctactacttc caccgggtgc tcgtggacgt gggcgacccc
      181 gtgctggcca cggccggcta caacgcgggg ccgggccggg ccaagcggtg gcgcgacgcg
      241 aagcccctgg agggcgcgat ctacgccgag acgataccgt tcaacgagac gcgcgactac
      301 gtgaaaaagg tcatggtgaa cgcgtggttc tatacccacc gcctcaccgg ccgcctggcc
      361 agcctgcatc gcctcatggg caccgtgccc ggccgcggac ccggcgaggc ggccaacagc
      421 gtggccgcca tcccgtgatg gacaacgcgc gcgttctcgt cctcggcgcc accggctttg
      481 tcggccgctc ggtctgcgcc cggctggaag cgcgcggcac cgggcttcgc gtgctcacgc
      541 gcgacctcct caaggcaagg ccgctgcagg tcattccctc gatcgagatc gtcaccggga
      601 gcccccacga cgacgcggac cttgcgcgtg ccctggacgg cgtggacgcg gcggtgaacc
      661 tcgtcggcat cctgcaccag agccgccgcg agccgttcga gaaggtgcac gtcgagttgc
      721 cgcggcgcct tgcgcgcgcc tgccgcgcgg cgggcgtgcg ccgcctcgtg cacatgagcg
      781 cgctgcacgc cgggcgcgac ggcccaagcg aatatctgcg ctcgcgcgga cgcggcgagg
      841 acgccatccg cgaggagggc gcggggctcg ccgtcaccat cttcaggccg tcggtcatct
      901 tcgggcagga cgacagcttc ctcaacctct tcgccgggct cgtgaggttc ctgcccgtcg
      961 ttccgctggc gggcgcgggc acgaagttcc agccggtctg ggtggaggac gtcgcacgga
     1021 ccgtggcggc atgcctggat gacccgcgca cctccgggaa gacctacgag ctttgcgggc
     1081 ccggcacgta cgcgctcgcg cagatcgtcg atttcgtggc cggactgcag ggcaggaaac
     1141 ggctcgtcgt gccccttccc gggtgggcgg cgcaggccca ggcctacgtg ctcgagcacc
     1201 tgccggggcc ggtcatgacg cgcgacaacc tcgcctcgat gaaggtggac agcgtgtgcg
     1261 gctgcccctt cccggaggag ctcggtttcg cccccgccac gatggaggca atcgttcctg
     1321 aatacatggc ggccgcggga cttcgtggcc gctacgcgga ctaccgccgt cgcggaggga
     1381 ggtgacatgg ccgacttgac gctcgtcatc ggcaacaaga actactcgtc gtggtcgatg
     1441 cggccctggg tgctgctgac gcagctgggc atcccgttcg aggagaggaa gctgcgcttc
     1501 aactccgacg aatgggacgc cggcatcggc cgctggtcgc cttcgggcct ggtgccggtc
     1561 ctgtggcggg gcaacgaggc ggtgtgggat tcgctcgcca tcatggaagc ggtggccgag
     1621 tggttcccgg acaagggtgt ctggcccgcc gacccggccg cgcgcgccct cgcgcgctcg
     1681 actgccgcgg aaatgcacgc gggctttcgc aacctgcgcg cgaagatgcc gatgagcatc
     1741 cgctcgcgct accccggcaa gggcatgacg ccggaggtgc gcgccgacat cgatcgcatc
     1801 gagcatctct ggcgcgatgc acggtcgcgc ttcggttccg gaggcgactt cctcttcggc
     1861 cgcttctcgg ccgccgacgc gatgttcgcc ccggtggtga tgcgtttcgc gacctacggc
     1921 gtcacctgcg ggccggtatc cgcgcgctat tgcagggcca tgcaggccgc agccggcgtg
     1981 cgcgcgtgga tcgagggcgc gcaacaggag acggaattcg tggccgagga cgagccctac
     2041 gcggaaccgc cgcggcc
//
LOCUS       DASUEV010000015         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1034452, whole genome shotgun sequence.
ACCESSION   DASUEV010000015 DASUEV010000000
VERSION     DASUEV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034452"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /gene="aroA"
                     /locus_tag="VFV55_00355"
     CDS             <1..930
                     /gene="aroA"
                     /locus_tag="VFV55_00355"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX4942771.1"
                     /translation="DLVDALRQAGARIAYLAKEGFPPLAIAPREGHGNDSVRVSGEVS
                     SQFLTALLIALGAAGGGIRVEVVGELISKPYVEMTLALMQRFGVRVEREGWSAFVVPA
                     GARYRSPGAIDVEGDASSASYFLAAGLLGGGPLRVEGVGRASLQGDVRFTEVIEAMGG
                     KVSFGDNWVEVSGTFPLAPLDADLNTIPDAAMTAAVLALFASGPSRLRNIASWRVKET
                     DRLFAMATELRKLGAKVEEGGDWLSIEPPAGFAGGAGTAQGRVAIDTYDDHRMAMCFA
                     LAAFGPVPIRINDPGCVSKTFPGFFEAWSRMVS"
     gene            943..1608
                     /gene="cmk"
                     /locus_tag="VFV55_00360"
     CDS             943..1608
                     /gene="cmk"
                     /locus_tag="VFV55_00360"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX4942772.1"
                     /translation="MKDVPVIAIDGPSASGKGTVAERVAAALGFHYLDSGALYRLATL
                     QALRRQVPLEDPEALAAAARAMEISFRDGTLSLDGEDVTQAVRTESVSAATSRVAASA
                     AVREALLERQRGFRRAPGLVADGRDMGSVVFPDATLKVFLTAEVAIRAERRHKQLMEK
                     GMCAKMCDVVEELRQRDARDSSRPVAPLKHYPDALFLDTSSLTIDQAVEQVLAWYRER
                     ARG"
     gene            1778..>2350
                     /locus_tag="VFV55_00365"
     CDS             1778..>2350
                     /locus_tag="VFV55_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX4942773.1"
                     /translation="MQVQTTNPTPASESFAALFEESLTRQEMRSGEVITAEVISVDPN
                     VVIVNAGLKSESAISTEEFKNDRGEIEVKPGDFVSVAIEALEDGFGATKLSREKAKKL
                     AAWSELDTALEGGKIITGVVSGKVKGGLTVMVNGIRAFLPGSLVDLRPMQDTSHFEGK
                     ELEFKVIKLDRKRNNVVVSRRAVMEETAGVE"
BASE COUNT          387 a          773 c          809 g          381 t
ORIGIN      
        1 gacctggtcg atgccctgcg ccaggccggt gcccgcatcg cttacctcgc gaaggagggc
       61 ttcccgccgc tcgccatcgc gccgcgcgaa gggcatggga acgactccgt gcgcgtgagc
      121 ggtgaggtat cgagccagtt cctcaccgcg ctgctcatcg cgctcggcgc ggcgggcggc
      181 ggcatccggg tcgaggtcgt cggcgagttg atttcgaagc cctacgtcga gatgacgctc
      241 gcgctgatgc agcgattcgg cgtccgtgtc gagcgggagg gctggagcgc attcgtggtt
      301 cccgcgggcg cccgctaccg ctcgcccggt gcgatcgacg tcgagggcga tgcctcctcg
      361 gcctcctact tcctcgcggc cggcctgctc ggtggcggcc cgctgcgcgt ggaaggcgtg
      421 gggcgcgcca gcctgcaggg cgacgtgcgc ttcacggaag tgatcgaggc gatgggcggc
      481 aaggtttcgt tcggggacaa ctgggtggag gtatcaggca cgttcccgct cgcgcccctc
      541 gacgcggatc tcaacacgat tcccgacgcg gcgatgaccg ccgccgtcct cgccctgttc
      601 gcgagcggcc cgtcgcggct gcgcaacatc gcgagctggc gcgtgaagga gaccgaccgg
      661 ctcttcgcca tggctaccga attgcgcaag ctcggcgcga aggtggaaga gggcggcgac
      721 tggctgtcca tcgagccgcc cgcgggtttc gccggcggcg cggggacggc gcagggacgc
      781 gtagccatcg acacctacga cgaccatcgc atggcgatgt gcttcgcgct cgcggccttc
      841 gggccggtgc cgatccggat caatgacccg ggatgcgtct cgaagacctt tcccggcttc
      901 ttcgaagcct ggtcgcgcat ggtctcgtag gcgatcgaaa cgatgaaaga cgttcccgtc
      961 atcgccatcg atggcccatc cgcctccggg aagggcacgg tggccgagcg cgtggcggct
     1021 gcgctgggct ttcactacct cgacagcggc gcgctgtacc ggctggctac cttgcaggcc
     1081 ctgcgccggc aggtgccgct ggaggatccc gaggccctgg cggccgccgc ccgcgccatg
     1141 gaaatatcct ttcgagacgg gacgttatcg ctcgatggcg aggatgtgac gcaggccgtg
     1201 cgcaccgaat cggtgagtgc cgccacctcg cgggtggccg cgagcgccgc ggtgcgcgag
     1261 gcgctgctcg agcgccagcg tggtttccgc cgggcgcccg gcctcgtcgc ggacgggcgc
     1321 gacatggggt cggtggtgtt cccggacgcg accctcaagg tgttcctcac ggccgaagtg
     1381 gccatccgcg cggaacgcag acataagcag ttgatggaaa agggaatgtg tgcaaaaatg
     1441 tgcgacgttg tggaggagct gaggcagcgt gacgcgcgcg acagcagcag gcccgtggca
     1501 ccgctaaagc attatcccga tgcgcttttc ctcgatactt cgagcctcac gatcgaccaa
     1561 gcggtcgagc aggtgctggc ctggtaccgg gaacgcgccc gcggataggt caaactctcc
     1621 ctgacggggc caaaagccca agggtgtcaa tcggttaggt ggtgtcccct ggttcgggcc
     1681 aagcccggga cccgggtgcc cgcccgccga tcggcgtttt ccaaccagac gcggccgcaa
     1741 ggccgtgccg aacgattttt ttttagggtt cccatccatg caggtccaaa ccaccaaccc
     1801 cacccccgcc tcagaaagct tcgccgccct cttcgaggaa agcctcacgc gccaggaaat
     1861 gcgctcgggc gaagtgatca ccgccgaggt catctcggtc gacccgaatg tggtgatcgt
     1921 gaacgcggga ctgaagtccg aaagcgcaat ttccaccgag gaattcaaga acgaccgcgg
     1981 cgagatcgag gtcaagcccg gcgacttcgt ctccgtcgcc atcgaggccc tcgaagacgg
     2041 cttcggcgcc accaagctct cgcgcgagaa ggccaagaag ctcgccgcgt ggtcggagct
     2101 cgacaccgcg ctggaaggcg gcaagatcat caccggcgtg gtatccggca aggtgaaggg
     2161 cggcctcacc gtcatggtga acggcatccg cgccttcctg cccggttcgc tcgtcgacct
     2221 gcggccgatg caggacacct cgcacttcga gggcaaggaa ctcgagttca aggtcatcaa
     2281 gctcgatcgc aagcggaaca acgtcgtcgt ctcgcgccgg gccgtcatgg aggagactgc
     2341 cggcgtcgag
//
LOCUS       DASUEV010000016         5243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_576534, whole genome shotgun sequence.
ACCESSION   DASUEV010000016 DASUEV010000000
VERSION     DASUEV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576534"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VFV55_00370"
     CDS             complement(<1..1384)
                     /locus_tag="VFV55_00370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4942774.1"
                     /translation="MQIRFLGAAREVTGSCFLVDTGRLRFTVDCGMHQGGREAGPKNH
                     AFPAFDPASLDFALVTHAHLDHCGLLPVLSSRAPALSIYTTRPTADLLPVMLLDSAHI
                     QGREKSHATRRRAPAPVPPLYGVREVERAMRQVSGVPYGVEFRPHPDARVRFLDAGHI
                     LGSAIAEVRVRDGGHERRIVFSGDLGQPARPVVRDPENVALADVLVVESTYGNRLHKS
                     MDDTVHELVGAVRDTLGNRRGNLIVPAFALGRAQELLILLFELCERGELEELNVFVDS
                     PLARQATAVTLAHLESLDPVVGRFAQAMRRRRLPFRLRFTRTPEESIAINSIRSGAIV
                     ISASGMCEAGRIRHHLRHNLAREECGILFTGFQAGGTLGRRIVDGARSVQLFGETVPV
                     RARIHTLGGLSAHADQRALLGWLSGFRRPPSRTYVVHGEEAAALDFAAAVESKLGWQV
                     RVPGPGETADC"
     gene            1555..2877
                     /locus_tag="VFV55_00375"
     CDS             1555..2877
                     /locus_tag="VFV55_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942775.1"
                     /translation="MPAGNLTMIRVTIQGIAIALVVGLSASATARAPEAKATATKATA
                     AKATAAKAPDASQKCIDCHSEAEPTDKSAAGKPVLLDPVKHAKTLHGSGKAGCVDCHA
                     DEAMRKYPHKSPKPAQCATCHDKAVKAYATTIHGMARADGKTVAASCSDCHGTHDIKA
                     ISDDAARTSRANLESTCGTCHGDEKLIREGKVPGGNVAAKYHDSIHGRLLRGDTKAKD
                     KVPVCTDCHGTHDMRPKRDPESAVARANIPETCGTCHAKVKKVWAGSQHGKLQATNVQ
                     IAPVCIDCHGTHTIADPKTPKWQVAVIKECGGCHTEFIKTYRDTYHGQVTDLGFARMA
                     TCASCHGAHEVLPKDNPMSKISPQRILATCQACHPTANANFVKFQPHANKNKKESGLI
                     LYYTTKFMQVLLAGVFAFFGLHTILWLYRSLAEMKAKRARPSPEAEKH"
     gene            2877..3746
                     /locus_tag="VFV55_00380"
     CDS             2877..3746
                     /locus_tag="VFV55_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942776.1"
                     /translation="MASQIQAVPGAARAGRNAAHKYYERFDRIDRVMHAFLMTTFIGC
                     AITGLPPLFSDHEWSAGLARVLGGFETAALIHRICAGVMILVWSLHVVRIFADAIHRT
                     GFLAMLWGPNSMVPQPQDIIDIWMHLKWFVGKGPRPQFDRWTYWEKFDYWAVFWGMAI
                     IGGSGLLLWFPSQFAAFLPGWVFNVATVVHGEEALLAVGFIFTIHFFNGHIRPEKFPM
                     DLVIFTGRIPEEELKDERPNEYRRLVSEGRLESIETTQPTPWTETFGYFVGGAAVILG
                     SLTVVFIIYSMIT"
     gene            3873..>5243
                     /locus_tag="VFV55_00385"
     CDS             3873..>5243
                     /locus_tag="VFV55_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX4942777.1"
                     /translation="MRIKLGRRTLMLGSVTTIVALVVGISVIGAAGIVGWEFSNSNAF
                     CANMCHSVHPEETAAHDVGVHARVNCVECHMGRNSTLHLMALKPTHAKELWGMIAGYE
                     RPIASGTLRPSREACESCHWPSVEHHDSIDVKVHYGTDPASGESRTRIVLHTGMEAIR
                     EGYTKGIHWHIQNEVRFVSPDPQRREIPWVEVRKPDGTKVSYTDGETKLTAQQISALE
                     PRAMACYDCHNAVGHPFPNPETTVDEAIRVGRIDRTLPSAKARAVALVNSLADVTGEL
                     KEREAKVDKLIADAAAKAATPAEHKQAEQKFNKAMREILLAASFSAKGFTWKSFPNHI
                     GHADTPGCFRCHDGKHYNDKGEAIRLQCTLCHALPQVTREDGKASVASLLPDKVGEKQ
                     PESHQRPNFMHTHSDDVGPECEKCHGPALKRGKQGGNFCANPACHGRTWPGMNLSVEP
                     PKQAAAE"
BASE COUNT          987 a         1764 c         1759 g          733 t
ORIGIN      
        1 agcagtccgc tgtctcgccg ggaccgggca cgcggacctg ccagcccagc ttcgactcca
       61 ccgcggcagc aaagtcgagg gcggcggctt cctcgccgtg gaccacgtac gtccgggaag
      121 gcgggcgccg gaatccggag agccagccca ggagcgcccg ctggtccgcg tgtgcggaaa
      181 gcccgccgag cgtgtggatc ctcgcccgca ccggcacggt ctcgccgaag agctgcacgc
      241 tgcgcgcgcc gtccacgatg cgccggccca gggtgcctcc ggcctggaaa ccggtgaaga
      301 ggatgccgca ctcctcccgc gccaggttgt gccgcaggtg atggcgtatc cgcccggcct
      361 cgcacatgcc gctcgcggag atcacgatcg cgccggaacg gatggaattg atcgcgatgg
      421 attcctcggg cgtccgggtg aaacgcagcc ggaagggaag acgccgccgg cgcatcgcct
      481 gcgcaaagcg gccgaccacg ggatcgagcg actcgaggtg cgcgagcgtc accgccgtcg
      541 cctggcgggc gagcggggaa tcgacgaaca cgttgagctc ctcgagttcg cctcgctcgc
      601 acagttcgaa aagcaggatc agcaactcct gcgcgcgacc gagcgcgaac gcgggcacga
      661 tgaggttgcc gcgccgattg cccagcgtgt cgcgcacggc gcccacgagt tcgtgcaccg
      721 tgtcgtccat cgacttgtgc agccggttgc catacgtcga ttcgacgacg agaacgtcgg
      781 cgagggccac gttctccggg tcgcggacca cgggacgtgc cggctggccc aggtcgcccg
      841 agaagacgat gcgacgctcg tggccgccgt cccggacacg cacctcggcg atcgccgagc
      901 cgaggatgtg ccccgcatcg aggaagcgca cgcgcgcatc gggatgcggg cggaattcca
      961 cgccgtaggg aacgccgctc acctggcgca tggcacgctc gacctcccgg acgccgtaga
     1021 ggggtggcac cggggctggt gcccggcgac gcgtggcgtg gctcttctcg cgcccctgga
     1081 tgtgggcgct gtcgagcagc attaccggca gcaggtcggc ggtggggcgc gtcgtgtaga
     1141 tggaaagggc cggcgcccgg gatgaaagca cgggcagcag gccgcaatgg tcgaggtggg
     1201 catgcgtgac cagcgcgaag tcgaggctcg ccggatcgaa tgcgggaaag gcgtggttct
     1261 tcggccccgc ctcgcgcccg ccctggtgca tgccgcaatc gacagtgaag cgcaggcgac
     1321 ccgtatcgac gaggaagcat gagcccgtca cttcgcgcgc cgcgccaagg aagcggatct
     1381 gcatggcgca attctaaccg ggaggcggcc acgcgccgcg aggctcctcg attgacccac
     1441 gtcaacattg catacccccc tccgtgagac ctttggccac tgaagaggtc cagtgccaat
     1501 gcccgcccgc atccgcagga tgcggggccc ggattccggg ccggggcgat ggccatgccc
     1561 gcagggaacc tgacgatgat ccgcgtgacg attcaaggca ttgcgatcgc ccttgtggtg
     1621 gggctttcgg cttcggcgac ggccagggcg cccgaggcca aggccacggc gaccaaggcc
     1681 acggcggcaa aggccacggc ggcaaaggct ccggacgcga gccagaaatg catcgattgc
     1741 cattccgagg ccgagcccac ggacaagagc gccgcgggca agcccgtcct cctggacccg
     1801 gtgaagcacg cgaaaaccct gcacgggtcc ggcaaggcgg gctgcgtcga ttgccacgcc
     1861 gacgaggcaa tgcgcaagta tccccacaag tcgcccaagc ccgcccagtg cgccacctgc
     1921 cacgacaagg cggtcaaggc atacgccacc acgatccacg gcatggcgcg cgccgacggc
     1981 aagacggtcg cggcttcgtg cagcgattgc cacggcaccc acgacatcaa ggcgatttcg
     2041 gacgacgccg cccgcaccag ccgcgccaac ctcgagtcga cgtgcggcac gtgccacggc
     2101 gacgagaagc tcatccggga gggcaaggtg cctggcggca acgtcgccgc gaaataccac
     2161 gacagcatcc acggaaggct cctgcgcggg gacaccaagg cgaaggacaa ggtgccggtg
     2221 tgcacggact gccacggcac gcacgacatg cgcccgaaga gggatccgga aagcgccgtc
     2281 gcacgcgcca acattcccga gacctgcggt acgtgccatg ccaaggtgaa gaaggtctgg
     2341 gcgggcagcc agcacggcaa gctccaggcg accaacgtgc aaatcgcgcc ggtatgcatc
     2401 gactgccacg gcacgcacac gatcgcggat ccgaagacgc cgaagtggca ggtcgccgtg
     2461 atcaaggaat gcggcggctg ccatacggaa ttcatcaaga cctatcgcga cacgtaccac
     2521 ggccaggtga ccgacctcgg gttcgcgcgc atggccacct gcgcgagctg ccacggcgcg
     2581 cacgaggtgc tgccgaagga caatccgatg tccaagatct ccccgcagcg cattctcgcc
     2641 acctgccagg cctgccaccc gacggccaac gccaacttcg tgaagttcca gccgcacgcc
     2701 aacaagaaca agaaggaaag cggcctgatc ctgtactaca ccaccaagtt catgcaggtg
     2761 ctgctcgcgg gcgtattcgc gttcttcggc ctgcacacga tcctgtggct ctaccggtcg
     2821 ctcgcggaga tgaaggcgaa gcgcgcccgg ccgtcccctg aagcggagaa gcactgatgg
     2881 ctagccagat ccaggccgta cccggcgcgg cgcgggcggg acgcaacgcc gcccacaagt
     2941 actacgagcg gttcgaccgc atcgaccgcg tgatgcacgc cttcctgatg accacgttca
     3001 tcggctgcgc gatcaccggg ctgccgccgc ttttctccga ccacgaatgg tcggcgggtc
     3061 tcgcgcgggt gctgggcggt ttcgagacgg cggctctcat ccaccgcatc tgcgcaggcg
     3121 tgatgattct cgtgtggtcc ctgcacgtgg tgcggatctt cgccgacgcg atccaccgca
     3181 ccggattcct ggcgatgctg tgggggccga actcgatggt cccgcagccg caggacatca
     3241 tcgacatctg gatgcacctg aagtggttcg tcggaaaggg tccgcgcccg cagttcgacc
     3301 gctggaccta ctgggagaag ttcgactact gggcggtctt ctggggcatg gccatcatcg
     3361 gcggctccgg gctcctgctg tggttcccgt cccagttcgc ggccttcctg cccggctggg
     3421 tcttcaacgt ggcgacggtg gtgcacgggg aggaagcgct tctcgccgtg ggattcatct
     3481 tcacgatcca cttcttcaac gggcacatac gccccgagaa attcccgatg gacctggtga
     3541 tcttcaccgg gcggatcccc gaggaagagc tcaaggacga gcgccccaac gagtaccggc
     3601 gactggtgag cgaaggcagg ctggagtcga tcgagacgac gcagcccacg ccatggacgg
     3661 agacgttcgg ctacttcgtg ggcggcgccg cggtgatcct cggctcgctc acggtcgtct
     3721 tcatcatcta cagcatgatc acttgatgcg gcgcggcatt acaatccgcg catcggggcc
     3781 ggcttgtccg gccccgcgat catcgtggcg gcaacacaac gggccggggc aggcatgcgc
     3841 cggcgaatga caacgagcgg gagtacggag ccatgaggat taaattgggt cgcaggacgc
     3901 tgatgctggg gtcggtcacg acgatcgtcg cgttggtcgt gggaatttcc gtgatcggcg
     3961 cggcgggaat cgtgggctgg gagttttcca acagcaacgc cttctgcgcc aacatgtgcc
     4021 actccgtcca ccccgaggag accgcagccc acgacgtcgg cgtgcacgcg cgcgtgaatt
     4081 gcgtcgagtg ccacatgggg cgcaactcga cgctgcacct gatggccctc aagcccacgc
     4141 acgccaagga gctgtggggc atgatcgccg gctacgagag gccgatcgct tccggcaccc
     4201 tgcgcccgtc gcgcgaggcg tgcgagagct gccactggcc gtccgtcgag caccacgaca
     4261 gcatcgacgt caaggtgcac tacgggaccg acccggcaag cggcgagtcg cgcacgagga
     4321 tcgtcctgca caccggcatg gaagcgattc gcgagggcta caccaagggg atccactggc
     4381 acatccagaa cgaggtgcgc ttcgtctcgc cggacccgca gcgccgcgag atcccctggg
     4441 tcgaagtgag gaagcccgac ggcaccaagg tcagctatac cgacggcgag accaagctca
     4501 ccgcccagca gatctcggcg ctggaaccga gggccatggc ctgctacgac tgccacaacg
     4561 ccgtcggcca tccgttcccc aaccccgaaa cgacggtgga cgaggcgatt cgcgtgggca
     4621 ggatcgatcg cacgctgccc agcgccaagg cgcgggccgt ggcgctcgtc aattcactgg
     4681 ctgacgtgac gggggaactc aaggagcgcg aggccaaggt cgacaagctc atcgcggacg
     4741 ctgccgcaaa ggccgccacg ccggcggagc acaagcaggc ggagcagaaa ttcaacaagg
     4801 cgatgcggga gatcctcctt gccgcctcgt tcagcgccaa gggcttcacg tggaaatcgt
     4861 ttccgaacca catcgggcac gccgacacgc cgggctgctt ccgctgtcac gacggcaagc
     4921 attacaacga caagggcgag gcgatccggc tgcaatgcac gctttgccac gcgctgccgc
     4981 aggttacgcg cgaggacggc aaggcaagcg tcgcctcgct actcccggac aaggtggggg
     5041 agaagcagcc cgagagccac cagaggccaa acttcatgca cacgcacagc gacgatgtcg
     5101 gtccggaatg cgagaagtgc cacggccccg ccctcaagcg cggcaagcag ggcgggaact
     5161 tctgcgccaa tcccgcctgt cacggccgca cgtggccggg aatgaatctg tcggtcgagc
     5221 cgcccaagca ggccgctgcc gag
//
LOCUS       DASUEV010000017         6894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_691419, whole genome shotgun sequence.
ACCESSION   DASUEV010000017 DASUEV010000000
VERSION     DASUEV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691419"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..2066)
                     /locus_tag="VFV55_00390"
     CDS             complement(93..2066)
                     /locus_tag="VFV55_00390"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX4942778.1"
                     /translation="MFAKILIANRGEIACRVIGTCRALAVRTVAVHSEADARALHVEM
                     ADEAYAIGGPRPADSYLRGDRIIAAAKASGAEAIHPGYGFLSENAAFARAVEAAGLVF
                     IGPTPEAMEKMGLKDRAKTLLEKAGVPVVPGYHGENQDDAYLRLQAAKIGFPLLVKAV
                     AGGGGRGMRLVAAAADFDTQLGAARREAEGAFGDGRVLLERFVQGPHHVEFQVFGDTH
                     GNYVHLFERECSIQRRHQKVLEESPSPFLDEPLRHRMGKAAVAAAKTIGYRGAGTVEF
                     IVGADRQFFFMEMNTRLQVEHPVTEMITGEDLVEWQLRVAAGEPLPLAQADILTGGHA
                     IEVRICAENPGNGFLPETGRIAVLRTPPEAPDVRLDTGIREGDTVSVFYDSMIGKLIA
                     WGDDRAEAARRLLSALARTEILGVRTNLSFLERLVAHPAFLAGETDTAFIEKHRADLL
                     PPAGEVPIEALVAAAVRVHRDERAAYTGSSPWDDAGGWRLNQPAARTMEFLRSDGETV
                     KVQADMGVGHAMVRVAGRSHRVVLGPVDGERLQISLDDETFFARVVRHGNLVSASTPA
                     GRHELQLVDPFHYEPADLIPDARLTALMPGRVVKLLAREGDTVAKGQSLMILEAMKME
                     HTLVSPREGVIERVAFAEGELVPADSLLIVFRD"
     gene            complement(2075..2467)
                     /locus_tag="VFV55_00395"
     CDS             complement(2075..2467)
                     /locus_tag="VFV55_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942779.1"
                     /translation="MFEAISIPFGARMLFNTLRLKPGVAMEDVELALGEMCNIVKNTY
                     GGDKGGFIAGQVFRFSGFASAEGSLGSPGATDDHIAIVTYWRSFEEHERSHADRVFKE
                     RFAALAALCTETRELGYELLWQGEPESP"
     gene            complement(2517..3575)
                     /locus_tag="VFV55_00400"
     CDS             complement(2517..3575)
                     /locus_tag="VFV55_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4942780.1"
                     /translation="MARKRAATLPASDRATVAAGFVTGLLSGLAARGIDRAVILREAG
                     IRPAAIAGPRSRVPLPAYAALYDAAIRASGDEGFGLFELPLRPGTFEFLCRAVIGSRR
                     LDEALDRASRFLALVLPQLHVAIRRDGETARLTIAEVRRLRPDPADPRRVFAFEWLLR
                     LLHALACWLAGRNLALDAVSFPYPRPAHAADYSLIYTEHSSFGAEALVATLDAALLEL
                     PVRRDDTDLAAFLDGAPGKITMLYRRDRELARSLRAILADALPQALDLAQAARELHVS
                     PRTLHRRLQDEGANFRAVKDALRRDLALARLAKPGMSVARIAADLGYSEPSAFFRAFQ
                     GWTGEAPSAWRRRFARRR"
     gene            3707..>6894
                     /gene="icmF"
                     /locus_tag="VFV55_00405"
     CDS             3707..>6894
                     /gene="icmF"
                     /locus_tag="VFV55_00405"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF045497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEX4942781.1"
                     /translation="MTDLSVRKKLATYKPAHKVRIVTAAALFDGHDASINIMRRILQG
                     MGAEVIHLGHNRSVEEIVTAALQEDAQGIAVSSYQGGHVEFFKYMMDLLRERGGAAIR
                     VFGGGGGVIVPAELKELHAYGVTRLFSPEDGQAMGLQGMIGSILAATDIDLSVQAPKS
                     LAALADPDPVLRWRALAQLITALELGKADPALRAEMIKAAASRRVPTLGITGTGGAGK
                     SSLTDELVRRLRLDQEDSLSIAVVSIDPSRRKSGGALLGDRIRMNAINPWSKGPRVYM
                     RSLATREAGNELSRALPDVLAACRCAGFDLIIVETSGIGQGDAAIVPLVDTSLYVMTP
                     EYGAASQLEKIDMLDFADFVAINKFDRKGAADALRDVSKQVQRNHQAFDRAPDAMPVF
                     GTMAARFNDDGVTALYQALLAKLKDIGLEAGPSRLPAVATRHSTNQTPIIPANRTRYL
                     AEIAEAVHGYKKRAAEQARLARERQQLSQAHRMLAAECHEENANLEALAADRDAKLDA
                     RSKKLLDLWPRMREAYSGDEYVVKIRDKEVRTALTSKSLSGSKIRKVALPHFEDHGEL
                     LRWLLLENVPGSFPFTAGVFAFKRENEDPTRMFAGEGDPFRTNRRFKMLSAGMAAKRL
                     STAFDSVTLYGNEPDPRPDIYGKVGNSGVSIATLDDLKVLYSGFDLTAPATSVSMTIN
                     GPAPTILAMFMNTAIDQNLDKFRADNGREPTDDEAQKIREWVLENVRGTVQADILKED
                     QGQNTCIFSTEFSLKVMGDIQQWFVHHGVRNFYSVSISGYHIAEAGANPLSQLAFTLA
                     NGFTYVEAYLARGMHIDDFAPNLSFFFSNGMDPEYTVLGRVARRIWAVAMRDKYGANE
                     RSQKLKYHCQTSGRSLHAQEIAFNDIRTTLQALIATYDNANSLHTNAYDEAITTPTEE
                     SVRRALAIQLVINREWGLAKNENPSQGSFIIEELTELVEEAVLKEFEAISERGGVLGA
                     MELGYQRGKIQEESLHYEHMKHTGEYPIIGVNTYRNPHGDPAHDKLELARSTEEEKKS
                     QLDRLHDFQRRHAAEAPAMLERLKHAVIANENV"
BASE COUNT         1177 a         2339 c         2373 g         1005 t
ORIGIN      
        1 ggagagcgca gcggccccat ctccccatcc ccggccttcc tcgcgttcgc cgcgctcgca
       61 cgtacagctg caacgcgacc cgaccccggc agctagtcgc ggaagacgat caggagggaa
      121 tccgcgggca cgagttcgcc ctcggcaaag gcgacgcgct cgatgacgcc ttcgcgcggg
      181 ctcaccaggg tgtgctccat cttcatcgct tcgaggatca tgagcgactg gcccttcgcg
      241 accgtgtcgc cttcgcgcgc gaggagtttc acgacgcgtc ccggcatcag cgccgtgagg
      301 cgcgcgtcgg gaatgaggtc cgcgggctcg taatggaagg gatcgacgag ctggagctcg
      361 tgccggcccg cgggcgtgga ggcggaaacg agattgccgt ggcgcaccac gcgagcgaag
      421 aacgtctcgt cgtccagcga gatctgcagc cgctcgccgt cgacggggcc gagcacgacg
      481 cgatggctgc gcccggcgac ccggaccatg gcatgaccca cgcccatgtc cgcctgcacc
      541 ttcacggtct cgccgtcgga gcgcaggaat tccatcgtgc gcgcggccgg ctggttgagg
      601 cgccatccgc ccgcgtcgtc ccagggcgat gaacccgtgt aggctgcgcg ctcgtcgcga
      661 tggacgcgga ccgcggcagc gaccagtgcc tcgatcggca cctcgcccgc cggcggcagg
      721 aggtccgcgc ggtgcttctc gatgaacgcc gtatcggttt cgcctgcgag gaaggcaggg
      781 tgcgcgacaa gccgctcgag aaaggacagg ttggtgcgca cgccgagtat ctcggtgcgc
      841 gcgagcgccg atagcagccg gcgcgcggcc tccgcccgat cgtcgcccca cgcgatgagt
      901 ttgccgatca tcgaatcgta gaagacgctc accgtgtcgc cttcgcggat gccggtgtcg
      961 aggcgcacgt cgggcgcctc gggcggcgtg cgcagcacgg caatgcggcc cgtctcggga
     1021 aggaatccgt tgccggggtt ctcggcgcag atgcgcactt cgatcgcatg cccgccggtg
     1081 aggatgtcgg cctgggcaag cggcaacggc tcgccggccg ccacgcgcag ttgccattcg
     1141 acgagatcct cgccggtgat catctccgtc accgggtgct cgacctgcag gcgcgtgttc
     1201 atctccatga agaagaactg gcggtcggcg ccgacgatga actccaccgt gccggcgccc
     1261 cggtagccga ttgtcttcgc ggccgcgacc gccgccttgc ccatgcggtg gcgaagcggc
     1321 tcgtcgagga agggcgaggg ggattcctcg agcaccttct ggtggcggcg ctggatggag
     1381 cactcgcgct cgaagaggtg cacgtaattg ccgtgcgtgt cgccgaagac ctggaactcg
     1441 acgtggtgcg ggccctggac gaatctctcg aggagcacgc gcccgtcgcc gaaggcgcct
     1501 tccgcctccc gcctggcggc gccgagctgg gtgtcgaaat cggccgcggc cgcgacgagg
     1561 cgcattcccc tgccaccgcc tcccgccacc gccttcacga ggagcggaaa accgatcttg
     1621 gcggcctgga ggcgaaggta ggcatcgtcc tggttctcgc cgtggtagcc gggcaccacc
     1681 ggaacgccgg ctttttccag cagcgtcttg gcgcggtcct tgaggcccat cttctccatg
     1741 gcctcgggcg tggggccgat gaagacgagt cccgcggcct ccactgcgcg cgcaaacgcg
     1801 gcgttttccg agagaaatcc gtatccggga tggatggcct cggctccgct ggccttcgcg
     1861 gcggcgatga tccggtcgcc gcgcaggtag gaatcggccg ggcgcggacc gccgatcgca
     1921 tacgcctcgt ctgccatctc gacgtgaagc gcgcgcgcat ccgcctcgga atggacggcg
     1981 acggtgcgca cggcgagcgc acggcaggtc ccgatgacgc ggcaggcgat ctcgccgcga
     2041 ttggcgatga ggatcttggc gaacatcgct tccgctaggg gctttccggc tcgccttgcc
     2101 agaggagttc gtacccgagt tcccgggttt ccgtgcacag ggcggcgagt gcggcgaatc
     2161 tttccttgaa cacgcggtcc gcgtgggagc gctcgtgctc ctcgaaggat cgccagtaag
     2221 tcacgatggc gatgtggtcg tcggtcgcgc cggggcttcc gagcgagcct tccgcggagg
     2281 cgaatccgga gaagcggaac acctgcccgg caatgaaccc gcccttgtcg ccgccgtacg
     2341 tgttcttcac gatattgcac atctcgccca gggcgagctc cacatcctcc atcgccacgc
     2401 cgggcttcag ccgcagcgtg ttgaagagca tcctggcccc gaagggaatc gaaatggctt
     2461 cgaacatgta tttcgctcct ggtcgcgtgg cgcagccatt gtacttcgcc cccgcctcat
     2521 cgccggcgcg cgaagcgccg gcgccaggca cttggcgctt ccccggtcca tccctggaag
     2581 gcgcggaaga atgccgaggg ctccgaatag cccaggtcgg cggcgatgcg tgccacgctc
     2641 atgcccggct tggcgagcct cgccagcgca aggtcgcggc gcagcgcgtc cttcacggcc
     2701 cggaagttcg cgccttcgtc ctgcaggcgg cgatgcagcg tgcgcggcga gacatgcagt
     2761 tcgcgggcgg cctgggcaag gtccagggct tgcggaagtg cgtcggcaag aatggcccgg
     2821 aggctgcgcg cgagctcccg gtcgcgccgg tagagcatcg tgatcttgcc cggggcaccg
     2881 tcgaggaacg ccgcgaggtc cgtgtcgtcc cgccggaccg gcaactcgag gagcgccgcg
     2941 tccagcgtgg ccacgagagc ctcggcgccg aaggatgaat gctcggtgta gatcaggctg
     3001 tagtccgcgg cgtgggccgg gcgagggtag gggaagctca cggcgtccag ggcgaggttg
     3061 cgtcccgcga gccagcacgc aagggcgtgc agcagccgca gcagccattc gaaggcgaag
     3121 acgcggcgcg gatccgccgg gtccgggcgc agccggcgga cttccgcaat ggtgaggcgc
     3181 gcggtttcgc cgtcgcgccg gatggccacg tgcagctgcg gcaggacgag ggcgaggaac
     3241 cgcgacgcgc ggtcgagcgc ctcgtcgagc cggcgcgacc cgatgaccgc gcggcaaagg
     3301 aactcgaagg tgccgggacg aagggggagc tcgaaaaggc cgaacccctc gtcgccgctg
     3361 gcgcgaatcg ccgcatcgta gagcgccgca tacgccggca acggaacccg ggatcgcggg
     3421 ccggcgatgg cggccggacg gatgcccgcc tcgcgcagga tgacggcgcg atcgatgccg
     3481 cgcgccgcga ggccggacag cagcccggtc acgaaaccgg cggcgacagt tgcacgatcg
     3541 gaggccggca gggttgccgc acgctttcgt gccattgcgt acgccttgga tgggctcgcg
     3601 ggatcagccg cggagattgg cggattttac acgatccgag tcgtcgccgg gcatggagtg
     3661 cccgggcacg gctggcctat gatggcgtca ggaacgagga gcgaccatga ccgacctttc
     3721 cgtgcgcaag aagctcgcga cctacaagcc ggcccacaag gtgcgcatcg tcaccgcggc
     3781 cgcgctcttc gacggccacg acgccagcat caacatcatg cggcgcatcc tgcagggcat
     3841 gggcgccgag gtgatccatc tcgggcacaa ccgttccgtc gaggagatcg tcaccgccgc
     3901 gctgcaggag gacgcgcagg gcatcgccgt gtcgagctac cagggcgggc atgtcgagtt
     3961 cttcaagtac atgatggatc tccttcgcga acgcggcggc gcggccatcc gcgtcttcgg
     4021 cggcggcggc ggcgtcatcg tccccgcgga gttgaaggag ctgcatgcat acggcgtgac
     4081 gcggctcttc tcgccggagg acggccaggc gatgggcctg cagggcatga tcggctccat
     4141 cctcgccgca acggacatcg atctctccgt gcaggccccg aagtcgcttg cggcgctggc
     4201 cgaccccgat cccgtcctgc gctggcgtgc actcgcgcag ctcatcacgg cgctcgagct
     4261 ggggaaagcc gacccggcgt tgcgcgccga gatgatcaag gcggccgcgt cccggcgcgt
     4321 gcccacgctc ggcatcaccg gcaccggcgg ggcaggcaag tcgagcctca ccgacgagct
     4381 cgtgcggcgg ctgcgactgg accaggagga cagcctctcg atcgcggtgg tttccatcga
     4441 tccatcgcgc aggaagagcg gtggggcgct cctcggcgac cgcatccgca tgaacgcgat
     4501 caacccgtgg tcgaaggggc cgcgcgtcta catgcgctca ctcgccacgc gcgaggcggg
     4561 caacgagctg agccgtgcgc tgcccgacgt gctggccgcg tgcaggtgcg ccggcttcga
     4621 cctcatcatc gtcgagactt ccggaatcgg ccagggcgat gcggccatcg tcccgctggt
     4681 cgacacgagc ctgtacgtga tgacgccgga gtacggggcg gcgagccagc tcgagaagat
     4741 cgacatgctc gacttcgcgg acttcgtggc catcaacaag ttcgaccgca agggcgcggc
     4801 cgacgcgctg cgcgacgtct cgaagcaggt gcagcgcaac caccaggcct tcgaccgtgc
     4861 ccccgacgcg atgcctgtct tcggcacgat ggcagcgcgc ttcaacgacg atggcgtgac
     4921 ggcgctgtac caggcgctgc tggcgaaact gaaggatatc gggctcgagg cggggccgtc
     4981 gcggctgccg gccgtcgcca cgcgccacag caccaaccag acgccgatca tcccggcgaa
     5041 ccgcacgcgc tacctggcgg agatcgccga ggccgtgcac ggctacaaga agcgcgccgc
     5101 ggagcaggcc cggcttgcgc gcgagcgcca gcagctctcg caggcgcacc gcatgctggc
     5161 cgccgagtgc cacgaggaga atgcgaacct cgaggcgctc gccgccgatc gcgacgcgaa
     5221 gctggacgcg cgctcgaaga agctcctcga cctgtggccg cgcatgcgcg aggcctacag
     5281 cggcgacgag tacgtggtga agatccgcga caaggaagtg cgcaccgcgc ttacctccaa
     5341 gtcgctctcg ggcagcaaga tccgcaaagt ggcgctgccg cacttcgagg atcacggcga
     5401 gcttctccgc tggctgctcc tggaaaatgt accgggttca ttccccttca ctgccggggt
     5461 gtttgcgttc aagcgcgaga acgaggaccc cacgcgcatg ttcgccggcg agggcgaccc
     5521 gttccgcacc aaccggcgtt tcaagatgct ctccgcgggc atggcggcca agcggctgtc
     5581 cacggccttc gattcggtga ccctctacgg caacgagccg gacccgcgcc cggacatcta
     5641 cggcaaggtg ggcaacagcg gggtgagcat cgccacgctc gacgacctga aggtgctcta
     5701 ttcgggcttc gacctcacgg cgccggcgac cagcgtcagc atgacgatca acggcccggc
     5761 gcccacgatc ctggcgatgt tcatgaacac cgccatcgac cagaacctgg acaagttccg
     5821 cgccgacaac gggcgcgaac ccaccgacga cgaggcgcag aagatccgcg agtgggtgct
     5881 ggagaacgtg cgcggcacgg tgcaggccga catcctcaag gaggaccagg gccagaacac
     5941 ctgcatcttc tccaccgagt tctcgctcaa ggtgatgggc gacatccagc agtggttcgt
     6001 gcaccacggc gtgcggaact tctattccgt ctcgatcagc ggctaccaca tcgccgaagc
     6061 cggggccaat ccgctctcgc agctcgcctt cacgctcgcc aacggcttta cctacgtgga
     6121 ggcttacctc gcgcgcggca tgcatatcga cgacttcgcg cccaacctga gcttcttctt
     6181 ctccaacggc atggaccccg agtacacggt gctcgggcgc gtggcgcggc gcatctgggc
     6241 cgtggcgatg cgcgacaagt acggcgccaa cgagaggagc cagaagctca agtaccactg
     6301 ccagacgagc ggacgcagcc tgcacgcgca ggaaatcgcc ttcaacgaca tccgcaccac
     6361 gctgcaggcg ctcatcgcga cctacgacaa cgccaacagc ctgcacacca atgcctacga
     6421 cgaggcgatc acgacgccga cggaggaaag cgtgcggcgc gcgctggcga tccagctcgt
     6481 catcaatcgc gagtggggac tggcgaagaa cgagaacccc agccagggct ccttcatcat
     6541 cgaggaactg accgagctgg tggaagaggc ggtgctcaag gagttcgagg cgatttccga
     6601 gcgcggcggc gtgctgggcg ccatggagct gggctaccag cgcggcaaga tccaggagga
     6661 gtccctgcac tacgagcaca tgaagcacac cggcgaatac ccgatcatcg gcgtgaacac
     6721 ctaccgcaac ccgcacggcg acccggcgca cgacaagctc gagcttgccc ggtcgacgga
     6781 agaagagaag aagagccagc tcgaccgcct gcacgacttc cagcggcgcc acgccgccga
     6841 ggcgccggcg atgctcgagc ggctgaagca cgcggtgatc gccaacgaga acgt
//
LOCUS       DASUEV010000018         8648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1605751, whole genome shotgun sequence.
ACCESSION   DASUEV010000018 DASUEV010000000
VERSION     DASUEV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605751"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="gatB"
                     /locus_tag="VFV55_00410"
     CDS             complement(<1..1057)
                     /gene="gatB"
                     /locus_tag="VFV55_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX4942782.1"
                     /translation="MQWETVIGLETHAQLSTASKIFSGASTAFGAAPNTQASAVDIAL
                     PGVLPVLNKAAVERAIRFGLSVGGSIAPRSVFARKNYFYPDLPKGYQISQYELPVVTG
                     GEILIAVDGVEKRVRLTRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDMR
                     SARQAVAYAKALHSLVRWIGICDGNMQEGSFRCDANVSVRPMGSGTLGTRCEIKNLNS
                     FRFLEKAIEHEVRRQVQLIEDGGRVRQETRLWDPQKGETRAMRSKEDAHDYRYFPDPD
                     LLPLEIAPSWIASVKASMTELPAAMRERFVSRHGLTAADATTLTASKALATYFEEVVR
                     LAGGEPKTCANWVLGELS"
     gene            complement(1060..2517)
                     /gene="gatA"
                     /locus_tag="VFV55_00415"
     CDS             complement(1060..2517)
                     /gene="gatA"
                     /locus_tag="VFV55_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX4942783.1"
                     /translation="MFDLTISALASLLRDRKASSEELTRLFLDRIARLDPGINAFITV
                     DEEKALASARVADARLARDDADALTGIPIAHKDLFCAKGWRTTCGSKMLANFISPYDA
                     HVVEQFDRAGAVLVGKTNMDEFAMGSSNENSFFGPVRNPWDPSRVPGGSSGGSAAAVA
                     ARLVPAATGTDTGGSIRQPASLTGTAGIRPTYGAVSRYGMVAFASSLDQGGALGKSAQ
                     DLALMLNAMAGFDERDSTSVERPKEDYARDLARPLAGLRVGLPKEYFTAELAPDVAAC
                     VAAALDELKRLGATTVEVSLPNQALAVPVYYVIAPAEASSNLSRFDGVRYGHRAADYA
                     DLNDMYRKSRAQGFGAEVQRRILVGTYVLSHGYYDAYYLQAQKVRRLIARDFTEAFKS
                     CDVIAGPAAPTVAFPIGEKTSDPVQMYLEDLYTISVNLAGLPGLSVPCGFGAGGLPVG
                     LQVIGNYFDEARMLNVAHQYQQATDWHCRRPPGID"
     gene            complement(2558..2842)
                     /gene="gatC"
                     /locus_tag="VFV55_00420"
     CDS             complement(2558..2842)
                     /gene="gatC"
                     /locus_tag="VFV55_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX4942784.1"
                     /translation="MLSLDEVRRIADLARIDVPEAGLAALQQQLNGIFGLIEQMRAVD
                     TAGVEPMAHAIDVTQRLREDRVTEADEHELFQSVAPQVEDGLYLVPKVLE"
     gene            2947..3990
                     /locus_tag="VFV55_00425"
     CDS             2947..3990
                     /locus_tag="VFV55_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX4942785.1"
                     /translation="MFSFVSKYFSTDLAIDLVTAKTLIYVRGKGIVLDEPSVVAIRQE
                     GGPNGKKTIQAVGLAAKQMLGRTPGNITAIRPMKDGVIADFTITEQMLKYFIKKIHDN
                     QWFRPSPRIVICVPYGSTQVERRAIKESAIGAGASQVFLIEEPMAAAIGADLPIAEAT
                     GSMVVDIGGGTTEVGVISLGGIVYSASVRVGGDKFDEAIINYIRRNYGMLIGESTAEQ
                     IKKKIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTDPLNSIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRELDRLLVEETGLPVIVADDPLTCVARGSGRAL
                     EEMDKLGTVFTND"
     gene            4006..4920
                     /gene="mreC"
                     /locus_tag="VFV55_00430"
     CDS             4006..4920
                     /gene="mreC"
                     /locus_tag="VFV55_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX4942786.1"
                     /translation="MIREPPPFFHRGPSPLARLTFFALLAIATMIADHRFGALDAVRL
                     SLSVLVAPLQSLAASPGMALARLGDYFSSQDRLLRENAELRARLLDYAAAAQQSKLLQ
                     AEQDHFLSMTPARSRFATDGVLAEVLSQGRNPFVRKLILDKGLAQGVKSGMPVIDGEG
                     VVGQVTAVGTFTSEATLLTEKDQSVPVMITRNGLRAIAVGSGKDGSIDVPFMPVSADI
                     QNGDLFVTSGIDGTYPAGLVVARVTSVEKNAAYVFARITAQPAAGVDNHRFVRILTAT
                     RPAIPSETRTEEKRPAKDRPAAKGRRPS"
     gene            4917..5450
                     /gene="mreD"
                     /locus_tag="VFV55_00435"
     CDS             4917..5450
                     /gene="mreD"
                     /locus_tag="VFV55_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX4942787.1"
                     /translation="MRPADLTPVARDVMKLPAGPGLVFASLVAAYLFNSLPWSGWALL
                     SRPDLVLVILVFWAIHQPGSIGQGLAFALGLAMDVGDSMLLGQHALAYVVAIYGAQVL
                     RVRVLAFAMLEQTLHVFGLFLGAAAVLVLLNLLLGATFPGLATLISPMLAALAWGPLT
                     WLLYLPGLRRMRRESAA"
     gene            5447..7348
                     /gene="mrdA"
                     /locus_tag="VFV55_00440"
     CDS             5447..7348
                     /gene="mrdA"
                     /locus_tag="VFV55_00440"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX4942788.1"
                     /translation="MIVGQALRDQRAELNTFRSRLMVAGFFALAAFGVLLARFVWLQV
                     IQREHYYTLAEANRISVVPVVPNRGLILDRNGQVLAANYSAYTLEITPSKVGNIEQSI
                     DELAQLIEVSPRDRRRFRRLQEESKNFESLPIRTRLTEEEVAKFAVNRFRFPGFEIKA
                     RLFRSYPHGDVASHAVGYIGRINDADVRRIESQGASTNYKGTDHIGKLGIEGAYEKEL
                     HGITGSERVEIDAGGRAIRALSRSEPQSGNNLVVTLDIKLQQVAEEAFQDYRGALVAL
                     DPKTGDVLALVSKPGFDPNLFVDGIDPVNWDALNNSPDKPLNNRALRGQYPPGSTIKP
                     FMALAALTYGKRTPEQAISDPGHWNLPGLARPFRDWKAGGHGMVNLYKSIVVSCDTYY
                     YGLANDLGIDAIHRFMTQNFGFGSRTGVDIEGEMPGLQPSTEWKWQRYKQKWYAGDTI
                     SVGIGQGYVLVTPMQLATAVATLANGGAPVHPRLVRAVQDARTQQLRELPSRNGGAVT
                     LKPEHLELVRAAMIDVTRPGGTAARAGQGAPYLVAGKTGTAQVVAMKAGEKYDEKRML
                     ERHRDHALFIAYAPADDPKIALALLVENGGHGSSTAAPIAREVFDYFLLHKRPAGRQP
                     LGGDEDEGD"
     gene            7341..8438
                     /gene="rodA"
                     /locus_tag="VFV55_00445"
     CDS             7341..8438
                     /gene="rodA"
                     /locus_tag="VFV55_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX4942789.1"
                     /translation="MISRLFDVISRRIDGPLMVALVLTLALGLTVVYSATGGASADRV
                     IAQSRNIAIAAAALWIVANIPPQALMRLAVPIYSAGLVLLVGVALFGEVRNGARRWLN
                     LGFTTIQPSEVMKIAVPLVLAWYFHRHQERIGLRDYAVAAILLALPVGLVVRQPDLGT
                     ALLVSASGFFVIFLAGLSWKVLATVFASGMVSLPFVWGMLHDYQRARVLTLLDPTEDP
                     LGAGYHIIQATIAIGSGGVLGKGWLNGTQAQLDFVPERSTDFILAVFGEEFGLAGNLV
                     LIVLYIIIVARGLMIAANASTTFARLMAGAVTLTFFTYAFVNMGMVSGILPVVGVPLP
                     MVSYGGTALLSMLAGFGILMSISTHKQLVSS"
     gene            8438..>8648
                     /locus_tag="VFV55_00450"
     CDS             8438..>8648
                     /locus_tag="VFV55_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     lipoprotein"
                     /protein_id="HEX4942790.1"
                     /translation="MRAALLVLVAAALAACGTAPKAPTPGKPAYYSDDGPPDKVPADI
                     ASIPDAVPRDEPYHRYANRPYTVFGR"
BASE COUNT         1346 a         3055 c         2843 g         1404 t
ORIGIN      
        1 ccgagagctc cccgagcacc cagttggcgc aggtcttcgg ctcgcccccc gccaggcgaa
       61 cgacttcctc gaaataggtc gccagcgcct tcgatgccgt gagcgtggtc gcatcggcgg
      121 cggtgagccc gtggcgggac acgaagcgct cgcgcatggc cgcgggcagc tcggtcatcg
      181 acgccttcac ggacgcgatc caggacggcg cgatctcgag cggcagcagg tccgggtcgg
      241 ggaagtagcg gtagtcgtgc gcgtcctcct tggagcgcat cgcgcgcgtc tcgcccttct
      301 gcgggtccca caggcgcgtc tcctggcgga ccctgccgcc gtcctcgatc aactgcacct
      361 gccggcgaac ctcgtgctcg atggcctttt cgaggaagcg gaaggagttg aggttcttga
      421 tctcgcaacg cgtcccgagc gtcccggaac ccatcggccg caccgacacg ttggcgtcgc
      481 agcggaagga cccttcctgc atgttgccgt cgcaaatgcc gatccagcgc accagcgaat
      541 gcagcgcctt cgcataggcc accgcctgcc gcgcgctgcg catgtccggc tcggagacga
      601 tctcgagcag cggcgtgccc gcgcggttca ggtcgatgcc gctcatgccg tggaagtcct
      661 cgtgcaggct cttgcccgcg tcctcctcga ggtgggcgcg cgtcaggcgc acgcgcttct
      721 cgaccccgtc cacggcgatg aggatctcgc caccggtgac gacgggcaac tcgtactggc
      781 tgatctggta gcccttggga aggtccgggt agaagtagtt cttgcgcgcg aagacgctcc
      841 tcggcgcgat gctcccgccc accgagaggc cgaagcggat ggcgcgctcg acggccgcct
      901 tgttcaggac cggcagcacg ccgggcagcg cgatgtccac cgccgatgcc tgcgtatttg
      961 gcgccgcgcc gaacgccgtc gacgcccccg agaagatctt cgaggcggtc gagagctggg
     1021 cgtgggtttc gagcccgatg acggtttccc attgcatggt caatcgatcc ccggcggacg
     1081 gcggcagtgc cagtcggtcg cctgctggta ctggtgcgcg acgttgagca tgcgcgcctc
     1141 gtcgaagtag ttgccgatca cctgcaggcc cacgggcagg ccgccggcac cgaagccgca
     1201 gggcaccgag agccccggca ggcccgcgag gttgaccgag atcgtgtaca ggtcctcgag
     1261 gtacatctgc accgggtcgc tcgtcttttc cccgatcggg aacgccaccg tgggcgcggc
     1321 ggggccggcg atcacgtcac aggacttgaa ggcctccgtg aagtcgcgcg cgatgaggcg
     1381 gcgcaccttc tgcgcctgca ggtaataggc gtcgtaatag ccgtgcgaga gcacgtaggt
     1441 acccacgagg atgcgccgct ggacttccgc gccgaaacct tgggcgcggc tcttgcggta
     1501 catgtcgttc aggtcggcgt agtcggccgc gcggtggccg tagcgcacgc cgtcgaaacg
     1561 cgacaggttc gacgaggcct cggccggtgc gatgacgtag tagacgggca ccgcgagcgc
     1621 ctggttgggc aggctcacct cgacggtcgt ggcgcccagt cgcttcaatt catcgagggc
     1681 cgcggccacg caggcggcga cgtcgggcgc gagttcggcc gtgaagtact ccttcggcag
     1741 ccccacgcgc aggcccgcca gcggcctggc gaggtcgcgc gcgtagtcct ccttcggccg
     1801 ctcgacgctg gtggaatcgc gctcgtcgaa gcccgccatg gcgttgagca tgagagcgag
     1861 atcctgcgcg ctcttgccga gcgcgccgcc ctggtcgagc gaggaggcga aggcgaccat
     1921 gccgtagcgc gaaaccgcgc cgtaggtggg acggatgccc gcggtgccgg taagggacgc
     1981 gggctggcgg atggagccgc ccgtgtcggt gcccgtggcc gcaggaacga gccgcgcggc
     2041 caccgcggcc gccgagccgc cggagcttcc gcccgggacc cgcgatgggt cccaggggtt
     2101 cctcaccggc ccgaagaagg agttctcgtt ggacgagccc atggcgaact cgtccatgtt
     2161 ggtcttgccc accagcaccg cgccggcgcg atcgaactgt tccaccacgt gcgcgtcata
     2221 gggcgagatg aaattcgcca gcatcttcga cccgcacgtc gtccgccatc ccttcgcgca
     2281 gaagagatcc ttgtgcgcga tcggaatgcc ggtgagcgca tccgcatcgt ccctggcgag
     2341 ccgggcgtcc gccacgcgcg ccgatgcgag cgccttttcc tcgtccacgg tgatgaacgc
     2401 gttgatgccc gggtcgagcc tcgcgatccg gtcgaggaag agccgcgtca gttcctcgct
     2461 cgaggccttg cggtcgcgca gaagggaagc aagggcggaa atcgtcaggt cgaacatgga
     2521 ttgtcgggga gcggcgacgc gcagcgccgc ggagaattta ctcgaggacc tttggcacga
     2581 ggtagaggcc atcctccacc tgcggcgcca cggactggaa cagttcgtgc tcatccgctt
     2641 cggtcacgcg gtcctcgcgc aggcgctgcg tcacgtcgat ggcatgggcc atgggctcca
     2701 cgccggcggt gtcgacggcg cgcatctgct cgatcaggcc gaagatgccg ttgagctgtt
     2761 gctgcagcgc agcaaggccg gcttccggga cgtcgatccg ggccaggtcg gcaatgcggc
     2821 ggacttcatc gagggacagc atccgaaaag cgctctaagg tcttaattca tcgtgatttt
     2881 gcgctattat aggcgacccc cgctgcctgg ggataggcac cagcccgatt tctcatacgg
     2941 atcaagatgt tctcattcgt ttccaagtac ttttccaccg acctcgccat cgacctcgtc
     3001 accgccaaaa cgctgatcta cgtgcgcggc aaggggattg tcctcgacga accgtcggtc
     3061 gtcgcgatcc gccaggaagg cgggcccaac ggcaagaaga ccatccaggc cgtcggcctc
     3121 gcggccaagc agatgctcgg gcgcacgccg ggcaacatca cggccatccg tcccatgaag
     3181 gacggcgtga tcgcggactt cacgatcacc gagcagatgc tcaagtactt catcaagaag
     3241 atccacgaca accagtggtt ccggcccagc ccgcgcatcg tgatctgcgt gccctacggc
     3301 tcgacgcagg tcgagcggcg cgccatcaag gaaagcgcca tcggcgcggg cgcgagccag
     3361 gtgttcctca tcgaggaacc catggccgcc gccatcggcg cggacctccc gatcgccgag
     3421 gccaccggct cgatggtggt cgacatcggc ggcggcacca cggaggtagg cgtgatctcg
     3481 ctgggcggga tcgtgtactc ggcttccgtg cgcgtcggcg gcgacaagtt cgacgaggcc
     3541 atcatcaatt acatccgccg caactacggc atgctcatcg gcgagtcgac cgccgagcag
     3601 atcaagaaga agatcggctc cgccttcccg ggcagcgaag tgcgcgagat ggaagtgaag
     3661 ggccgcaacc tcgctgaagg cataccgcgc tcgttcacga tctcgtccaa cgagatcctc
     3721 gaagcgctca ccgatccgct caactccatt gtctccgccg tgaagagcgc cctcgagcag
     3781 acgccgcctg aactcggcgc cgacatcgcc gaaaagggca tggtgctcac cggcggcggg
     3841 gcgctcctgc gcgaactcga ccgcctcctc gtggaagaga cgggcctgcc ggtcatcgtt
     3901 gccgacgatc ccctcacctg cgtggcccgc ggctccggcc gcgcgctcga ggagatggac
     3961 aagctcggaa cggtgttcac gaacgactga tcccctgccg cccgcgtgat tcgcgagccg
     4021 cctccgttct ttcatcgcgg tccgagcccg ctcgcgcgcc tcacgttctt cgcgctgctg
     4081 gcgatcgcga cgatgatcgc cgaccatcgc ttcggcgccc tcgatgccgt tcgcctgtcc
     4141 ctctcggtcc tcgtcgcccc cctgcagagc ctcgccgcct cccccggaat ggcgctcgcg
     4201 cgcctgggag actacttttc gtcgcaggac cggctgctgc gcgagaacgc cgaactcagg
     4261 gcgaggctcc tcgattacgc ggccgcggcg cagcagtcga agctcctgca ggcggaacag
     4321 gatcatttcc tctccatgac gccggcaagg agccgcttcg ccacggacgg cgtgctggcc
     4381 gaggtgcttt cccaggggcg caatcccttc gtgcgcaagc tcatcctcga caaggggctt
     4441 gcgcagggcg tcaagtcggg catgccggtc atcgacggcg agggcgtggt cgggcaggtg
     4501 acggcggtgg gcaccttcac gagcgaggcc acgctcctca ccgagaagga ccagtccgtc
     4561 ccggtgatga tcacgcgcaa cggcctgcgt gcgatcgccg tgggctcggg caaggacggc
     4621 tccatcgacg tgcccttcat gcccgtgtcg gcggacatcc agaacggcga tctcttcgtc
     4681 acctccggca tcgacggcac ctatcccgcg gggctcgtgg tggcccgcgt cacctcggtc
     4741 gagaagaacg cggcgtacgt gttcgccagg atcaccgcgc agcccgcggc cggcgtggac
     4801 aaccaccgct tcgtgcgcat actcaccgcg acgcgtcccg cgatcccgtc cgagacgcgc
     4861 accgaggaaa agcggcccgc caaggacagg ccggccgcaa agggccgtcg accgtcatga
     4921 gacccgccga cctcacgccc gtcgcgcgcg acgtcatgaa gcttcccgcg gggccggggc
     4981 tcgtcttcgc ctcgctcgtt gccgcgtacc tgttcaactc gctgccctgg tccgggtggg
     5041 cgctgctctc gcggccggac ctcgtgctgg tcatcctggt cttctgggcg atccaccagc
     5101 cgggctcgat cggccagggc ctcgccttcg ccctcggcct cgccatggac gtgggggatt
     5161 ccatgctcct cggccagcac gcgctcgcct acgtggtggc gatctacggc gcgcaggtgc
     5221 tgcgcgtgcg cgtgctcgcg ttcgcgatgc tcgagcagac gctgcacgtg ttcggccttt
     5281 tcctcggcgc ggcggccgtg ctcgtgttgc tgaacctcct cctgggcgcc acctttccgg
     5341 ggctggccac gctgatctcg ccgatgctcg ccgcgctcgc gtgggggccg ctcacctggc
     5401 tgctctacct gcccgggctt cgccgcatgc gccgggagtc ggcggcatga tcgtcgggca
     5461 ggcgctgcgc gaccagcgcg cggagctcaa cacattccgc tcgcgcctca tggtggcggg
     5521 gttcttcgcc ctcgccgcgt tcggcgttct cctcgcccgc ttcgtctggc tgcaggtgat
     5581 ccagcgcgag cactactaca cgctcgccga ggcgaaccgc atctccgtgg tgcccgtcgt
     5641 gcccaaccgc ggcctcatcc tcgaccgcaa cgggcaggtg ctcgccgcca actactccgc
     5701 ctacacgctc gagatcaccc cgagcaaggt gggcaacatc gagcaatcca tcgacgagct
     5761 cgcgcagttg atcgaagtga gcccgcgcga ccgccggcgc ttcaggcggc tgcaggagga
     5821 gagcaagaac ttcgagagcc tgcccatccg cacgcgcctc acggaggagg aggtggcgaa
     5881 gttcgcggtg aaccgcttcc gcttccccgg attcgagatc aaggcgcggc tcttccgctc
     5941 ctacccccac ggcgacgtcg cctcgcacgc ggtgggctac atcggccgca tcaacgacgc
     6001 cgacgtgcgc cgcatcgagt cgcagggcgc ctccaccaac tacaagggca ccgaccacat
     6061 cggcaagctc ggcatcgagg gcgcctacga gaaggaactg cacggcatca cgggcagcga
     6121 gcgggtcgag atcgacgccg gcggtcgtgc catccgggcg ctgtcgcgct ccgagccgca
     6181 gtcgggcaac aacctcgtcg tcacgctcga catcaagctg cagcaggttg cggaagaggc
     6241 cttccaggat tatcgcggtg cgctcgtggc gctcgacccg aagacgggcg acgtgctggc
     6301 gctcgtctcc aagccgggct tcgaccccaa cctcttcgtc gacggcatcg acccggtgaa
     6361 ctgggacgcg ctgaacaact cgcccgacaa gccgctcaac aaccgggcgc tgcgcggcca
     6421 gtacccgccg ggctccacga tcaagccgtt catggcgctg gccgccctca cctacggcaa
     6481 gcgcacgccg gaacaggcca tctcggaccc cggccactgg aatctgcccg gactcgcgcg
     6541 cccgttccgc gactggaagg cgggtggcca cggcatggtg aacctgtaca agtcgatcgt
     6601 cgtttcctgc gacacgtatt actacggcct ggccaacgac ctcggcatcg atgcgatcca
     6661 tcgcttcatg acgcagaatt tcgggttcgg ctcgcgcacc ggcgtggaca tcgagggcga
     6721 gatgccgggg ctgcaaccct cgaccgagtg gaaatggcag cgctacaagc agaagtggta
     6781 tgccggcgac acgatcagcg tgggcatcgg gcagggctac gtgctcgtca ccccgatgca
     6841 gctcgccacc gccgtggcca ccctcgccaa tggcggcgcg ccggtgcacc cccgcctggt
     6901 gcgcgcggtg caggacgccc ggacgcagca actgcgggag ctgccctccc gaaatggcgg
     6961 cgcggtaacc ctcaagcccg aacacctcga gctggtgagg gccgccatga tcgacgtgac
     7021 ccgccccggc ggcacggcgg cgcgcgccgg gcagggcgct ccctatctcg tcgccggcaa
     7081 gaccggaacc gcgcaggtcg tggccatgaa ggcgggcgag aagtacgacg agaagcgcat
     7141 gctcgagcgc catcgcgacc acgcgctctt catcgcctac gccccggccg acgacccgaa
     7201 gatcgcgctc gccctcctcg tcgagaacgg cggccacggc agctccaccg ccgcgccgat
     7261 cgcgcgcgag gtgttcgact acttcctgct gcacaagcgc ccggcgggca ggcagcccct
     7321 gggcggcgac gaggacgagg gtgattagcc ggctcttcga cgtcatctcg cggcgcatcg
     7381 acggcccgct catggtcgcg ctcgtgctca ccctcgccct gggcctcacc gtggtctatt
     7441 cggccacggg cggcgcctcc gcggaccggg tgatcgcgca gtcgcgaaac atcgccatcg
     7501 cggccgccgc gctctggatc gtggcgaaca tcccgccgca ggcgctgatg cgcctggcgg
     7561 ttcccatcta ttccgccggc ctcgtgctcc tggtcggcgt tgcgctcttc ggcgaggtgc
     7621 gcaatggcgc ccggcgatgg ctcaacctcg gtttcaccac gatccagccc tccgaagtca
     7681 tgaagatcgc ggtcccgctg gtgctggcgt ggtacttcca ccgccaccag gagcggatcg
     7741 ggctgcgcga ctatgccgtg gccgccatcc tgctggcgct gcccgtgggc ctcgtcgtcc
     7801 gccagccgga cctcggcacg gcgctcctcg tctccgcctc gggattcttc gtgatcttcc
     7861 tcgcgggcct ttcgtggaag gtcctcgcca ccgtcttcgc gagcgggatg gtgagccttc
     7921 ccttcgtgtg gggcatgctg cacgactacc agcgggcgcg ggtgctcacg ctcctcgacc
     7981 caaccgagga cccgcttggc gcgggctacc acatcatcca ggcgacgata gccatcggct
     8041 ccggcggcgt gctcggcaag ggctggctga acgggacgca ggcacagctc gacttcgttc
     8101 ccgagcgctc caccgacttc atcctcgcgg tgttcggcga ggagttcggt ctggcgggaa
     8161 atctcgttct catcgtcctc tacatcatca tcgtggcgcg cgggctcatg atcgccgcca
     8221 acgcctcgac caccttcgcg cgcctcatgg cgggggcggt aacgctgacc ttcttcacct
     8281 acgccttcgt gaacatgggc atggtgagcg ggatcctgcc ggtggtgggc gtgccgctgc
     8341 cgatggtgag ctacggaggc acggcgctgc tctcgatgct cgcgggcttc ggcatcctga
     8401 tgtcgatctc cacgcacaag cagctggtgt cgagctgatg cgcgcggccc tcctggtgct
     8461 cgtcgccgcg gccctggccg catgcggcac ggcaccgaag gccccaacgc ccggcaagcc
     8521 tgcctactat tccgacgacg gtccgccgga caaggtgccg gccgacatcg cctccattcc
     8581 cgatgccgtc cctcgcgacg agccctatca ccgctacgcg aaccgcccct acacggtatt
     8641 cgggagaa
//
LOCUS       DASUEV010000019         7052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_346155, whole genome shotgun sequence.
ACCESSION   DASUEV010000019 DASUEV010000000
VERSION     DASUEV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346155"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1538)
                     /locus_tag="VFV55_00455"
     CDS             complement(192..1538)
                     /locus_tag="VFV55_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4942791.1"
                     /translation="MGRTRGGGGPVRAAGRGPEGTARAFHRPPRIPASQAHDLKAETI
                     AGETAAAARLRRILYAAAFLRALAIGMMAVLIGLYCARLGFSAAQIGVVLSAALWGAA
                     LATLATMLAGRRFSERSLLLALTGLPVLGCAVLLATDVFAVIAAAAFAGMFNVNGRDR
                     GAIPILEQAMFPATTTDADRTRVFAWYNVLLDSGYAAGGLLAGIPTILESLAGMQTLA
                     AMKATLSLFCVLYLASAVLYARLPARVGDTAPAGLRQLSRESRPIIGKISALFFVDAF
                     AGGFIGSAIFAYWFSERFGASAATVAILFSAGRLLSAASHIVAAWLAKRIGLINTMVY
                     THIPSSLLLFTIVVADDFAWAAFFFLLRESLNEMDVPTRQSYVMAVVKPEERLAAAGL
                     TNLVRSGGWALAPMFAGTLMQTAGLGVPLVLAGIAKIGYDLALWGEFRRVKPPEER"
     gene            complement(1421..2500)
                     /locus_tag="VFV55_00460"
     CDS             complement(1421..2500)
                     /locus_tag="VFV55_00460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX4942792.1"
                     /translation="MRPTPLPPLLAVLVLAFASTAFGAASPSAAGKPAAIEKPDAVGK
                     EDAIEKEDDATYRQLQEAAGAVVAVHARALLNARSNESLGSERSGSGIVIAPSGLVLT
                     IGYLILEADQVEVTDANGRSLPASVVAYDHATGFGLLRTIGPISPKPVRLGNSGSVEQ
                     LDRLMIATGGEGGVSIATVVSRRPFAGYWEYLIDGAIFTAPPRMDHSGAALINKDGEL
                     VGIGSLFVMDAMKPGERLPGNMFVPIDLLKPILAEMIETGRQKAGRRPWIGVSSAEDD
                     GRLKVLRVTTDGPADKAGIHPGDIILAVGGEKVRDLPDFYHKLWASGEPGVEVGLSVL
                     QGADLKELRVRSIDRLEFLRRKPTI"
     gene            2581..4416
                     /locus_tag="VFV55_00465"
     CDS             2581..4416
                     /locus_tag="VFV55_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX4942793.1"
                     /translation="MSTATVPQEPARPAEPPVTGDAGVIRKLLPYLREYRGRVFLAIA
                     FLVTAKFANLAVPLVMKGIVDGLSGPKAVLAVPFALLAAYGLLRMSSTLFNELRDIVF
                     AKVAQRAMRRVALEVFEHMHSLSLRFHLERQTGGLTRDIERGTRGISTLLNFMVFSVL
                     PTFFEIALVTGFLIVRFDVWFGVITVGALCLYIAATFLISERRIHHRRTMNEMDSKAN
                     TRAIDSLINYETVKYFGNEAFEARRYDENLSRYERAVVTSETSLGFLNTVQAAIIALT
                     VSLLMWRASDGIVNGGLTLGDLVMVNALLIQLYIPLNFLGVLYREIKQSLTDMERMFR
                     LLGVNREVQDKPGAPALVVSGGAIRFEGVDFSYDGRRQILSGVDFEVPAGKNVAVVGA
                     SGSGKSTLGRLLFRFYDVQKGRITIDGQDIRDVTQVSVRSAIGIVPQDTVLFNDTIYY
                     NIAYGRPGAARDEVVEAAKVAHILEFIERLPDKWETSVGERGLKLSGGEKQRVSIART
                     LLKNPPVMIFDEATSALDSKTEKAIQAEFAEISRNRTTLMIAHRLSTIVDADQILVMD
                     AGRIVERGSFRELMALGGRFAEMWQLQQQEEAEDDPAVAAEGSAR"
     gene            4413..4826
                     /locus_tag="VFV55_00470"
     CDS             4413..4826
                     /locus_tag="VFV55_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942794.1"
                     /translation="MSSVNALRIPAVAFAASLALAIPGCAGPAPQAREGAVTVSKVER
                     VRWDLPPEPGIFRVRFDAAIAGEIRQQAERVAGSSDMDYYYAEIRVIEQEAGRELKAR
                     GLCPGSAKLVSPVESGDGKSGIGALFRCQVPLFRD"
     gene            complement(4862..5650)
                     /locus_tag="VFV55_00475"
     CDS             complement(4862..5650)
                     /locus_tag="VFV55_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4942795.1"
                     /translation="MNFIVQGYPAYAYTGGRTFDPKLPAVVFVHGAALDHSVWQWQSR
                     HLANHGFSVLAVDLPGHGRSPGTLRTSVEALADWVAAFIEAACVDQAHVVGHSMGSLV
                     ALDTALRHPQRVAKLVLLGASVPMPVGEAFLAAAKDDSPAAFDMQTVWGHARLSALAA
                     SPVPGASLAMAGRWLTARSHAGVQHADLAACNAYAPAKDALRELKVPTLVIAGKRDQM
                     TPLRAGQALAKEIPGARFLAFDAGHSLPTEAPREIAHALRAALA"
     gene            complement(5647..6945)
                     /locus_tag="VFV55_00480"
     CDS             complement(5647..6945)
                     /locus_tag="VFV55_00480"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX4942796.1"
                     /translation="MAHSGPKTPHPDTLAVHAGQSPDPATGARAVPIYQTASYVLPDA
                     DTAAALFNMELPGHVYSRLSNPTIAVFEERMAALEGGAGAIATASGQAAMHLAIATLM
                     GQGGHIVSSASLYGGSHNLLHYTLPRFGIETTFVPARDIDAWRRAVRPDTRLLFGETV
                     GNPSIDVLDIPAIAAIADENAIPLLVDATVTTPYLGRALDMGAHLVMHSATKFLSGHG
                     VVIGGVLVDGGRFDWDASGKFPTLTEPYEGFHGMVFSEESTVSAFLLRARREGLRDFG
                     ACMAAMTAFQILQGLETLHLRMPRHVASTATLVEFLAGHEAVARVYHPDATTHPDHAL
                     AARLYPNGCSAILSFELKGGREAGRKFIEALTLFSHLANIGDAKSLVIHPASTTHFRM
                     DDAALAAAGITAGTVRLSIGLEHPDDLVEDLSRALRASQK"
BASE COUNT         1121 a         2365 c         2493 g         1073 t
ORIGIN      
        1 gggtggaaag tcgaggccag acgtttttgg ctggcctcga ctttccgggc acccattcga
       61 cggtcacgcc tgcgtgccca ggattgcgat cgacttccac cgccgccgcc tcacctcacc
      121 tgcccaagat tgcgatcgac gtccaccgcc gccgccttct cgacccgcgt gcccggaatt
      181 cggtgaagag cctatctttc ctccggcggc ttgacgcgcc ggaattcgcc ccagagcgcg
      241 aggtcgtagc cgatcttcgc gatccccgcc agcacgagcg gcacgcccag ccccgccgtt
      301 tgcatgaggg tgcccgcgaa catcggcgca agcgcccagc cgcccgaacg cacgaggttg
      361 gtgaggccgg ctgccgcgag gcgctcctcc ggcttcacca ccgccatcac gtaggactgg
      421 cgcgtgggaa cgtccatctc gttgagcgac tcgcgcagca ggaagaaaaa ggcggcccac
      481 gcgaagtcgt cggccaccac gatggtgaac agcagcaggc tcgacgggat gtgcgtgtag
      541 accatcgtgt tgatgagccc gatgcgcttc gccagccacg cggcgacgat gtgggacgcc
      601 gccgagagca gccgcccggc gctgaagagg atggcgaccg tcgcggcgga ggcgccgaag
      661 cgctcggaaa accagtaggc gaagatcgcc gagccgatga agccccccgc gaaggcatcg
      721 acgaagaaga gcgccgagat cttgccgatg atcggccggc tttcccggga cagttgccgc
      781 aaccccgccg gcgcggtgtc gccgacgcgg gccggcaggc gcgcataaag caccgcggag
      841 gcgagataga gcacgcagaa gagggacagc gtcgccttca tggccgcgag cgtctgcatc
      901 ccggcaagcg actcgaggat cgtggggatc cccgcgagaa gtccgcctgc cgcgtagccg
      961 ctgtccagca gcacgttgta ccaggcgaac acgcgcgtgc ggtccgcgtc cgtcgtggtc
     1021 gcgggaaaca tggcctgctc caggatcggg atcgcgcccc ggtcgcggcc gttcacgttg
     1081 aacatgcccg cgaaagcggc agccgcgatg acggcgaaca cgtcggtggc gaggagcacg
     1141 gcgcaaccca ggaccggcag gccggtgagg gcgaggagta acgatcgctc ggaaaagcgc
     1201 cgcccggcga gcatcgtggc gagggtggca agggcggcgc cccacaacgc ggccgagagc
     1261 acgacgccga tctgcgcggc ggaaaagccc agccgagcgc agtagaggcc gatcagcacg
     1321 gccatcatcc cgatggcgag cgcacgcagg aacgcggcgg cgtacaggat gcggcgcagg
     1381 cgcgccgcgg cggcggtctc cccggcgatg gtctccgcct tcagatcgtg ggcttgcgac
     1441 gcaggaattc gaggcggtcg atggaacgca cgcgcagttc cttcaggtcc gcgccctgca
     1501 gcacggacag gcccacctcc accccgggtt cgcccgatgc ccagagcttg tggtagaagt
     1561 ccggcaggtc gcgcaccttt tccccgccga cggcgaggat gatgtcgccc gggtggattc
     1621 cggccttgtc cgcggggccg tcggtggtca cgcgcagcac cttgaggcgt ccgtcgtcct
     1681 cggcggaact cacgccgatc cacggccgcc ttcccgcctt ctgccggccc gtctcgatca
     1741 tttccgccag gatgggcttc aggaggtcga tggggacgaa catgttgccg ggcaggcgct
     1801 cgccgggctt catcgcgtcc atcacgaaga gcgagccgat gcccaccagt tccccgtcct
     1861 tgttgatgag cgcggcgccg ctgtggtcca tgcgcggcgg cgcggtgaag atcgcgccgt
     1921 cgatgaggta ttcccagtag cccgcgaagg gtcgtcgcga gacgaccgtg gcaatcgaaa
     1981 cgccgccttc cccgccggtc gcgatcatga gccggtcgag ctgctcgacg gaaccggagt
     2041 ttcccagccg gacgggcttg ggcgaaatcg ggccgatggt gcgcagcagg ccgaacccgg
     2101 tggcgtggtc gtaggccacg acgcttgccg gcagcgagcg gccgttggcg tcggtgacct
     2161 cgacctggtc cgcctcgagg atgaggtagc cgatggtgag gacgaggccg gagggcgcga
     2221 tcacgatccc ggaccccgag cgctcggacc cgaggctctc gttggagcgc gcgttgagca
     2281 gcgccctcgc gtgcaccgcc accaccgcgc cggccgcctc ctggagctgc cggtaggtgg
     2341 cgtcgtcttc cttctcgatg gcgtcttcct tcccgacggc gtcgggcttc tcgatggcgg
     2401 cgggcttccc ggcggcggag gggctcgccg cgccgaatgc cgtcgatgcg aaggccagga
     2461 ccaggactgc gaggagcggc ggaagggggg tcggacgcat gatcgcctcc tctggagaga
     2521 gatgcgcgcg aaggcatcct agcaccgctg ctactattag gggcgaagca ccacgcaccg
     2581 atgagcaccg ccaccgtccc gcaggaaccc gccaggcccg ccgagccccc ggtcacgggc
     2641 gacgccgggg tcatccggaa gctgctgccc tacctgcgcg agtaccgggg gcgcgtcttc
     2701 ctcgccatcg ccttccttgt caccgccaag ttcgccaatc tcgcggtgcc gctggtcatg
     2761 aagggcatcg tcgacgggct ctccgggccg aaggcggtcc tcgccgtgcc cttcgcgctg
     2821 ctcgcggcgt atgggctgct gcgcatgtcg tccacgctct tcaacgagct tcgcgacatc
     2881 gtgttcgcga aggtggcgca gcgcgccatg cgccgcgtgg cgctggaagt cttcgagcac
     2941 atgcattcgc tctcgctgcg cttccatctc gagaggcaga cgggcgggct cacgcgcgac
     3001 atcgagcgcg gcacgcgcgg catctcgacg ctgctcaact tcatggtctt cagcgtgctg
     3061 ccgaccttct tcgagatcgc gctggtgacc ggcttcctca tcgtgcgctt cgacgtgtgg
     3121 ttcggggtca tcacggtggg tgccctgtgc ctctacatcg cggccacgtt cctcatctcc
     3181 gagcggcgca tccaccaccg ccgcacgatg aacgagatgg actcgaaggc caacacgcgg
     3241 gccatcgact cgctcatcaa ctacgagacg gtcaagtatt tcggcaacga ggcgttcgag
     3301 gccaggcgct acgacgagaa cctctcccgc tacgagcggg cggtggtcac gagcgagacc
     3361 tcgctcgggt tcctgaacac cgtccaggcg gcgatcatcg ccctcacggt gtcgctgctc
     3421 atgtggcgcg cctccgacgg gatcgtgaac ggcggcctca cgctgggcga cctggtgatg
     3481 gtgaacgcgc tcctcatcca gctctacatc ccgctcaatt tcctcggcgt cctctatcgc
     3541 gagatcaagc agtcgctcac ggacatggag aggatgttcc gcctgctggg cgtgaaccgc
     3601 gaggtccagg acaagcccgg ggcgccggcg ctcgtggtgt ccggtggcgc catccgcttc
     3661 gagggcgtcg atttcagcta cgacggcagg cgccagatcc tgtccggggt cgacttcgag
     3721 gtgccggcgg gcaagaacgt cgccgtggtg ggcgcgagcg gttccggcaa gtcgacgctg
     3781 gggcggctgc tcttccgctt ctacgacgtg cagaagggcc gcatcaccat cgacgggcag
     3841 gacatccgcg acgtgacgca ggtatccgtg cgcagcgcca tcggcatcgt cccgcaggac
     3901 acggtgctgt tcaacgacac gatctactac aacatcgcct acgggcgtcc gggcgccgcg
     3961 cgcgacgagg tggtcgaggc ggcgaaggtc gcgcacatcc tggagttcat cgagcggctg
     4021 cccgacaagt gggagaccag cgtgggcgag cgcggcctca agctttccgg cggggagaag
     4081 cagcgcgtgt cgatcgcgcg cactctcctc aagaacccgc cggtcatgat cttcgacgag
     4141 gccacttctg ccctcgactc gaagaccgag aaggccatcc aggccgaatt cgcggagatc
     4201 tcgcgcaacc gcacgacgct catgatcgcg caccgtctct ccacgatcgt cgacgcggac
     4261 cagatcctgg tgatggatgc gggcaggatc gtggagcgcg gcagctttcg cgagctgatg
     4321 gcgctcggcg ggcggttcgc ggagatgtgg cagttgcagc agcaggagga agcggaagac
     4381 gatccggcgg ttgccgccga agggagtgcc cgatgagtag cgttaatgcc ttgcgtatcc
     4441 cggccgtagc cttcgcagcg tcgctcgcgc tcgcgatccc gggttgcgcc ggaccggcgc
     4501 cccaggcgcg ggaaggcgcg gtcaccgtct cgaaggtcga gcgggtccgc tgggacctgc
     4561 ccccggagcc gggaatcttc cgcgtgcgct tcgacgcggc catcgcgggc gaaatccggc
     4621 aacaggccga gcgcgtggcg gggtccagcg acatggacta ctactacgcg gaaatccgcg
     4681 tcatcgagca ggaagcgggc cgcgagctga aggcgagggg gctttgtccc ggcagcgcga
     4741 agctcgtctc ccccgtggag tccggcgatg ggaagtcggg gatcggcgcc ctgttccgct
     4801 gccaggtgcc cttgttccgg gattgaagcg gcttcccgcg cccggcgcct cccggcggga
     4861 gtcaggcaag cgcggcgcga agcgcgtgcg caatctcgcg cggggcctcc gtggggagac
     4921 tgtggcccgc gtcgaaggcg aggaacctgg cgcccgggat ctccttcgcg agcgcctgtc
     4981 cggccctcaa aggcgtcatc tggtcgcgct ttccggcgat caccagcgtc ggtaccttca
     5041 gttcgcgcag cgcatccttc gccggcgcgt aggcattgca ggccgcgagg tcggcatgct
     5101 gcacgcccgc gtgcgagcgg gcggtgagcc agcgcccggc catggcgagc gaggcgccgg
     5161 gcacggggct cgccgccagc gccgatagcc gcgcgtggcc ccataccgtc tgcatgtcga
     5221 acgcggcggg cgaatcgtcc ttcgccgccg cgaggaaggc ttcgccgacg ggcatcggca
     5281 ccgacgcgcc gaggagcacc agcttcgcca cgcgctgcgg atggcgcagc gcggtgtcga
     5341 gcgccaccag cgatcccatg ctgtggccga cgacgtgcgc ctggtccacg cacgccgcct
     5401 cgatgaacgc cgctacccag tcagccagtg cctcgacgga ggtgcgcaag gtgcccgggc
     5461 tgcgcccgtg gccgggcagg tcgacggcca gcacggaaaa gccgtggtta gcgaggtggc
     5521 gcgactgcca ctgccacacg ctgtggtcga gcgcggcgcc gtggacgaag acgacggcgg
     5581 gcagcttcgg gtcgaaggtc cggccgcccg tgtaggcata ggcggggtag ccctgcacga
     5641 tgaagttcat ttctgcgaag cgcggagcgc gcgcgaaaga tcctcgacca ggtcgtcggg
     5701 gtgctccagg ccgatcgaca gtcgcaccgt cccggcggtg atgcccgccg ccgcgagcgc
     5761 cgcgtcgtcc atgcggaaat gcgtcgtgga ggcggggtgt atcacgagcg acttggcgtc
     5821 gccgatgttg gccaggtgcg agaacagcgt gagcgcctcg atgaacttgc gccccgcctc
     5881 gcgcccgccc ttgagctcga aggacaggat cgcgctgcac ccattcgggt agaggcgcgc
     5941 ggcgagcgca tggtccgggt gggtcgtcgc gtccgggtga tagacgcgcg ccacggcctc
     6001 gtgcccggcc aggaattcga ccagcgtcgc ggtgctcgcc acgtgccgcg gcatgcgcag
     6061 gtgcagcgtc tccagcccct gcaggatctg gaacgcggtc atggccgcca tgcaggcgcc
     6121 gaagtcgcgc aggccttcgc ggcgcgcgcg gaggagaaat gccgaaacgg tcgactcctc
     6181 gctgaacacc atgccgtgga agccctcgta gggctcggtg agggtgggga atttcccgga
     6241 agcgtcccag tcgaaacgcc ccccgtcgac cagcacgccg ccgatcacca cgccgtgtcc
     6301 cgaaaggaac ttcgtcgccg agtgcatcac caggtgcgcg cccatgtcga gggcgcggcc
     6361 gaggtagggc gtggtgaccg tcgcgtcgac gagcagaggg atggcgttct cgtccgcgat
     6421 cgccgcgatg gccggaatgt ccagcacgtc gatggaaggg ttgccaaccg tctcgccgaa
     6481 caggagccgc gtgtccggcc gcacggcgcg ccgccacgcg tcgatgtcgc gcgcgggcac
     6541 gaatgtcgtt tcgatgccga agcggggcag ggtgtagtgc agcaggttgt gcgagccgcc
     6601 gtagaggctc gccgacgaca cgatgtgccc gccctggccc atgagggtcg cgatcgccag
     6661 gtgcatggcg gcctggccgc tggcggtggc gatggcgccg gcgcccccct cgagcgccgc
     6721 catgcgctcc tcgaacaccg cgatcgtcgg gttggaaagg cgcgagtaga cgtgccccgg
     6781 cagctccatg ttgaagagcg ccgccgcggt gtccgcatcg ggcaggacgt agctcgccgt
     6841 ctggtagatg ggcacggcgc gcgcaccggt ggccgggtcg ggcgactggc cggcatgcac
     6901 cgcgagggtg tcggggtggg gcgtcttggg tcctgaatgg gccatcgaat cagggatttg
     6961 gaatttgttc ttgatgcgcc acatattgtc acggacggca catgtttgct atggttcgtc
     7021 atccccgcca aatctgggtt cccatgaagc gc
//
LOCUS       DASUEV010000020         4671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1494233, whole genome shotgun sequence.
ACCESSION   DASUEV010000020 DASUEV010000000
VERSION     DASUEV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494233"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFV55_00485"
     CDS             complement(<1..275)
                     /locus_tag="VFV55_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4942797.1"
                     /translation="MARTYVIANQKGGVGKTSIAVNLSASLAHYGRRVLLVDLDPQGN
                     ATTGSGVDKTALDRTVYGILLGECSVAEARARAEGGYDVVGSNRELAG"
     gene            complement(275..901)
                     /gene="rsmG"
                     /locus_tag="VFV55_00490"
     CDS             complement(275..901)
                     /gene="rsmG"
                     /locus_tag="VFV55_00490"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX4942798.1"
                     /translation="MTIEARLADGLAAMGITLDAAAQAKLLAYVRLIEKWNKVHNLTA
                     VRDPGQMVALHILDSLSVLAHVAGARTLLDVGSGAGLPGIPLAIARPDLAVTLLDASH
                     KKATFLRQAKAELALANVEVACERVEKWHPAAPFDIVVSRAFAELAEFVEQAGHLVAR
                     DGTMLAMKGVNPIDEIARLPSSHRVGNVVELRVPSLDAKRHLVFLRAA"
     gene            complement(898..2805)
                     /gene="mnmG"
                     /locus_tag="VFV55_00495"
     CDS             complement(898..2805)
                     /gene="mnmG"
                     /locus_tag="VFV55_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX4942799.1"
                     /translation="MDFQTRFDVIVVGGGHAGTEAALAAARAGSRTLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVREVDALGGAMALAADEAGIQFRTLNASKGPAVRATRAQADRV
                     LYRAAIRRRLENQPNLQLFQQAVDDLILEGDRVAGAVTAIGLRFHAPAVVLTAGTFLG
                     GRIHVGLENYAAGRAGDPPSLSLAARLRELSLPVGRLKTGTPPRLDGRSIDFSRCAEQ
                     PGDCPEPVFSYLGSRDMHPRQLPCWITHTNEATHEVIRGGLDRSPMFTGVIEGVGPRY
                     CPSIEDKIHRFADKASHQVFLEPEGLTTHEYYPNGISTSLPFDVQWALVRTIPGLENA
                     HILRPGYAIEYDYFDPRGLKSTLETKAITGLFFAGQINGTTGYEEAAAQGLLAGINAS
                     LHARGEAGWCPRRDEAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLQLREDNADLRLTP
                     AGRRLGLVDDARWAAFSTKREAIERETARLKATWAHPGRIAEADQVRVLGQPIEREHS
                     LFDLLRRPDVSYRSLLSFPNAGDGVEDAAVAEQVEIAAKYAGYIDRQRDEISRQLAQE
                     TLTLPADFDYASVRGLSKEVQQKLTQQRPGTVGQAARIQGITPAAISLLLVHLKRRAL
                     ADRARPEERSA"
     gene            complement(3128..4474)
                     /gene="mnmE"
                     /locus_tag="VFV55_00500"
     CDS             complement(3128..4474)
                     /gene="mnmE"
                     /locus_tag="VFV55_00500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX4942800.1"
                     /translation="MSARPDTIAAIATAPGRGGIGVVRVSGPLAGDIARAVTGRIPEA
                     RRATLVRFAGAGGALLDEGIALYFPAPHSYTGEDVLELHGHGGPVVLLAIVRRCVALG
                     ARLAQPGEFTRRAFLNERLDLAQAEGVADLIDASSAEAARAAARSLTGEFSVRIEAIV
                     GALTRLRMHVEACIDFPEEEIDPADREGQRKALAAIRADLAELREEAAQGAVLREGLT
                     VVLVGRPNVGKSSLLNRLAGEDVAIVTAMAGTTRDYVRATILIEGVPIHLIDTAGLRE
                     AGDEAERIGIARTWEAIAKAGAAMLITDAAQSEREEESRILARMPQGLPLARVSNKID
                     VAGLAAGREAHGAEVCIRLSAKTGEGVGALRAWLLEVAGWRPSSEGVFLARERHLVAL
                     REAASQLDMAADKHGQAFELFAEDLKLAQRALGSITGEVSPEELLGQIFSRFCIGK"
     gene            complement(4479..>4671)
                     /locus_tag="VFV55_00505"
     CDS             complement(4479..>4671)
                     /locus_tag="VFV55_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX4942801.1"
                     /translation="QPADPVQAKVMLAMPIVFSIFFLFFPSGLVLYWVVQNLLSIAQQ
                     WHINRVIAAEAAAKARGKR"
BASE COUNT          723 a         1595 c         1631 g          722 t
ORIGIN      
        1 ccggcgagct cgcggttgga gccgacgacg tcgtagccgc cctccgcgcg cgcccgcgcc
       61 tcggccacgc tgcattcgcc gagcaggatg ccgtagacgg tgcggtccag cgcggtcttg
      121 tcgacgccgc tgccggtcgt ggcgttgccc tgcgggtcca ggtccacgag cagcacgcga
      181 cggccatagt gggccaggct cgccgaaaga ttcaccgcga tggatgtctt gccgacgccg
      241 cccttctggt tggcgatcac gtaggtacgg gccatcaggc ggctctcagg aagaccaggt
      301 ggcgtttggc gtcgagactc ggcacgcgca gctcgaccac gttcccgacg cggtgggagg
      361 agggcaacct ggcgatttcg tcgatggggt tcacaccctt catcgcgagc atcgttccgt
      421 cgcgcgcgac gaggtgtcct gcctgctcga cgaactccgc gagctccgcg aaggcgcgcg
      481 agacgacgat gtcgaacggg gcggccggat gccatttctc gacgcgttcg caggccacct
      541 cgacgttggc gagcgccagc tccgccttgg cctggcgcag gaaggtggcc ttcttgtggc
      601 tcgcgtcgag cagcgttacc gcgaggtccg gccgcgcgat cgcgagggga atgccgggga
      661 gccccgcgcc gctgcccacg tcgaggagag ttctcgcgcc ggcgacatgg gcaaggaccg
      721 agaggctgtc gagaatatgc agcgccacca tctgcccggg gtcgcgcacc gcggtgaggt
      781 tgtgcacctt gttccacttc tcgatgagcc gcacataggc caggagcttc gcctgggcgg
      841 ccgcgtcgag cgtgatgccc atggccgcca ggccgtcggc aagtcgtgcc tcgatcgtca
      901 cgcgcttctc tcctcgggcc gcgcgcgatc ggccaacgcg cggcgcttca ggtgcacgag
      961 cagcagcgag atggccgccg gcgtgatgcc ctggatgcgc gcggcctggc cgacggtccc
     1021 gggacgttgc tgggtaagtt tctgctgcac ttccttcgag agcccgcgca ccgacgcata
     1081 gtcgaagtcg gcaggaagcg tcagggtttc ctgggccagc tggcgcgaaa tctcgtcgcg
     1141 ctggcggtcg atgtagccgg catacttggc cgcaatttcc acctgctctg ccacggctgc
     1201 atcctcaacc ccgtcgccgg cattgggaaa ggacaacagt gagcgatagc tcacatcagg
     1261 acgccgaaga aggtcgaaaa gcgagtgctc gcgttctatc ggctggccta gaacgcgcac
     1321 ctggtcggcc tccgcaatgc gcccgggatg cgcccaggtg gccttcaggc gggccgtctc
     1381 cctctcgatg gcctcgcgct tggtgctgaa agcagcccaa cgtgcgtcgt ccaccagacc
     1441 gagccggcgg ccggccgggg tgagccggag gtcggcgttg tcctcgcgca attgcaaccg
     1501 gtattccgcc cggctggtga acatgcggta gggctccgac accccgcgcg tcacgagatc
     1561 gtccaccagg acgccgaggt aggcctcgtc gcgccgcggg caccagccgg cctctccgcg
     1621 agcgtgaagc gaggcgttga tcccggcgag caacccctgg gcggccgctt cctcgtagcc
     1681 ggtggtgccg ttgatctggc ccgcgaagaa aagcccggtg atcgccttgg tctcgagcgt
     1741 cgacttgagg ccgcgcgggt cgaagtagtc gtattcgatt gcgtagccgg gccgcaggat
     1801 gtgcgcattt tcgaggccag ggatggtgcg cacgagcgcc cactggacgt cgaagggcag
     1861 gctggtcgag atgccgttcg ggtagtactc gtgggtcgtg aggccctccg gctcgaggaa
     1921 gacctgatgg ctcgccttgt ccgcgaaccg gtggatcttg tcctcgatcg acgggcagta
     1981 gcgcgggccc acgccctcga tcacgcccgt gaacatggga gagcggtcga gtcccccgcg
     2041 aatcacttcg tgggtcgctt cgttggtatg cgtgatccag cagggaagct ggcgaggatg
     2101 catgtcacgc gagccaaggt acgagaacac cggttcgggg caatcgcccg gctgctccgc
     2161 gcacctggag aaatcgatac tgcggccatc gagccgcggc ggcgtgccgg tcttcaaccg
     2221 cccgacggga agcgagagtt cgcgcagccg agcggccagc gacagcgacg gcgggtcgcc
     2281 cgcgcgcccg gccgcgtagt tctccaggcc gacgtggatc cttccgccaa ggaaggtgcc
     2341 cgccgtgagg accaccgccg gcgcgtggaa tcgcagcccg atggccgtga ccgcgccggc
     2401 cacgcgatcg ccctcgagga tcaggtcgtc gacggcctgc tggaaaagct ggaggttggg
     2461 ctggttttcc aggcgccgcc ggatggcggc gcggtagagg acgcgatcgg cctgggcacg
     2521 cgtggcgcgc accgccggtc ccttgctcgc attgagggtg cggaactgga tgcccgcttc
     2581 gtccgccgca agcgccatgg cgccgcccag cgcatccact tcgcgcacca ggtgaccctt
     2641 gccgatcccg ccgatcgagg ggttgcagga catctggccc agggtttcga tcgagtgcgt
     2701 gagcaggagc gtgcgcgaac ccgcccgggc cgcggcgagc gcggcttcgg tgccggcgtg
     2761 gccgccgccg acgacgatca cgtcgaaacg ggtctggaag tccatggaaa caatcgggag
     2821 ccggggcgcg tccggcggcg gaacagcgaa tcgggaattc tacgggaaaa gccgcgagcg
     2881 acctagcgag gttccacgtg gaacagggtc cccggcggtc cgctcctggc ggccacccgg
     2941 ccgtcagcgc gatcgtcctt ttcgcccgcg tcgggttgca tgcccatatt ggcgccacgt
     3001 ccgccgcact ggcggagcgg gactggcggg ggcaggccga caagaccgtg cgggcaggtg
     3061 ttccacgtga aacccggccg ggtcgaagcc ggcacgcgga gtgacgagga acctgccggg
     3121 cgctctccta cttgccgatg cagaaccggc tgaaaatctg ccccagaagc tcttccgggc
     3181 tcacttcacc ggtgatcgaa cccagggctc gttgtgccaa cttcagatct tctgcaaaca
     3241 attcaaaagc ttgaccatgt ttgtcggccg ccatgtcgag ctgcgacgct gcctcccgca
     3301 acgccaccag gtggcgttcc cgggccagga acaccccctc cgatgacggg cgccatccgg
     3361 ccacttcgag cagccaggcc cgaagcgcgc ccacgccctc gccggtcttc gcggacagcc
     3421 ggatgcacac ctccgccccg tgcgcttcgc gcccggccgc gagccccgcc acatcgatct
     3481 tgttgctgac gcgcgcgagc ggcaggccct gcggcatgcg agcgaggatg cgcgactcct
     3541 cctcgcgttc actctgcgcc gcatccgtga tgagcattgc ggcccccgcc ttggcgatgg
     3601 cctcccaggt gcgcgcgatt ccgatcctct cggcctcgtc gcccgcctcg cgcaagcccg
     3661 ccgtgtcgat gaggtggatg ggaacgcctt cgatgaggat ggtggcccga acgtagtccc
     3721 gcgtggtccc ggccatggcc gtgacgatcg ccacgtcctc gcccgccagg cggttgagca
     3781 ggctcgactt gcccacgttg ggcctgccca cgagcacaac ggtgaggccc tcgcgaagaa
     3841 ccgccccttg ggcggcctcc tcgcgcagct cggccaggtc ggcgcggatg gccgcaagcg
     3901 ccttgcgctg cccctcacga tccgccggat cgatctcctc ctcgggaaaa tcgatgcagg
     3961 cctcgacgtg catgcgcagc cgcgtgagcg cgccgacgat cgcctcgatg cgcacggaga
     4021 actcgccggt gagcgaacga gcggcggcgc gcgcggcttc tgcgctcgac gcatcgatca
     4081 ggtcggccac gccctccgcc tgcgcgaggt cgaggcgctc gttgaggaaa gcgcgccgtg
     4141 tgaactcgcc gggttgggcg agccgcgcgc cgagcgccac gcatcggcgc acgatcgcga
     4201 gcagcacgac cgggccgccg tgcccgtgca gctcgagcac atcctcgccg gtgtacgagt
     4261 gcggggcggg aaagtagagg gcgatgccct cgtcgagcag cgcacctccg gcgcctgcga
     4321 atcgcacaag agttgcgcga cgggcctcgg gaatgcggcc cgtgacggcc cgggcgatgt
     4381 cgcccgcgag cgggccggaa acgcgcacga cgccgatgcc gccgcggccg ggtgccgtcg
     4441 cgatggccgc gatggtgtcc ggccgggcgc tcacggcgtt agcgcttgcc ccgtgccttc
     4501 gccgcagcct cggccgctat cacgcggttg atgtgccact gctgggcaat cgacagaagg
     4561 ttctgcacga cccagtagag cacgagaccg gagggaaaga agaggaagaa gatgctgaag
     4621 acgatgggca tcgccagcat caccttcgcc tgcaccgggt cggccggctg c
//
LOCUS       DASUEV010000021         9920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_578404, whole genome shotgun sequence.
ACCESSION   DASUEV010000021 DASUEV010000000
VERSION     DASUEV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578404"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(214..307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            503..1435
                     /locus_tag="VFV55_00510"
     CDS             503..1435
                     /locus_tag="VFV55_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX4942802.1"
                     /translation="MSILVCGSIAYDTIMVFRDQFKNHILPDQIHILNVAFLVPDMRR
                     EYGGCAGNIAYGLKLLGEEPFIMATVGHDFEPYGRRLDTLGLSKRHVRLLDEQFTAQA
                     FITTDIDDNQITAFHPGAMAQSHLNRIADTSGMRLAIVSPDGRQGMVEHARDLARARI
                     PYVLDPGQGLPMFSGDDVLEMLAGARCLTVNDYEARLVEQKTGKTIAELATRVEAVVV
                     TRGAEGADILSGGQQIHVPAVKPDSLVDPTGCGDAFRAGLLYGMARGWDWRRCGRLAS
                     LMGSIKIAYRGGQNHRVSRDEIERRFGDAFGERL"
     gene            1484..1945
                     /locus_tag="VFV55_00515"
     CDS             1484..1945
                     /locus_tag="VFV55_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX4942803.1"
                     /translation="MQARSLGTALVATALLGVAGCAAPPRGGADYRGYETRTEQSVRF
                     GVVESVRGVRINPTETGVGTASGAVLGGIAGSTIGHGRGSAAGAVAGAVLGGVIGSAV
                     EKDANDRQGIEITVMLDGGKYIAVVQEADESFRVGDRVRILSGRGTTRVTH"
     gene            2026..2754
                     /locus_tag="VFV55_00520"
     CDS             2026..2754
                     /locus_tag="VFV55_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4942804.1"
                     /translation="MLRVAWPRASAQRRHEIAVQWSQDLLAILAVRVHCDGKPPSQGR
                     TGAMIAANHVSWVDIFAIASVRHTRFIAKSEIRDWPLAGWLAAKSGTIFIRRARRHDT
                     ARINAQVHDALAEGECVALFPEGTTTEGDRLLKFHSSLFEPAVANGARVYPVAVRYED
                     RDGTPLRSAAYVGDLTFAQSLGLVIRTRETIARLAFAEPIHPERLSRQDVAMEAHRRV
                     ATLLGLPCAGTAPAKAADRPGEPR"
     gene            complement(2681..3502)
                     /locus_tag="VFV55_00525"
     CDS             complement(2681..3502)
                     /locus_tag="VFV55_00525"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX4942805.1"
                     /translation="MALYAIGDLQGCLEPLERLVEAIRFDPDRDRLWFVGDLVNRGPD
                     SLGCLRFVKSLGPAAITVLGNHDLHLLCVAEGIEKPRPRDTLEGVLAAPDRDELVDWL
                     RNLPLMHVEGAFAMVHAGLLPQWPVAKARELAGEVEAVLRGPGWRRFLARLYGNEPAR
                     WNDALAGEDRLRAIVNAMTRLRVCTPEGTMDLAFKGEPGERHSHRIPWFDMPGRANAT
                     HTIVCGHWSALGLHVGDKVLSLDSGCVWGNALTAVRLGDRRLWQVPCPRTGDPAE"
     gene            complement(3569..4165)
                     /locus_tag="VFV55_00530"
     CDS             complement(3569..4165)
                     /locus_tag="VFV55_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4942806.1"
                     /translation="MSQAYHSDAVKANPDSHPLKLAIWIVIGAVALIVGIILLAQFAI
                     GVYGGRSLENDPAMAREALAKRIAPVARLQVETAAAAAPAGAPAAAPAGAPVTQGPKV
                     ATVAAPAAPAAAAKAAKADGKKVYDTTCMACHSTGVAGAPKLGDKAAWAPRIATGLDA
                     LYGSALKGKAAMPPKGGNAALPDADVKAAVDYMVGGSK"
     gene            4294..4370
                     /locus_tag="VFV55_00535"
     tRNA            4294..4370
                     /locus_tag="VFV55_00535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4328..4330,aa:Arg,seq:ccg)
     gene            complement(4386..6254)
                     /locus_tag="VFV55_00540"
     CDS             complement(4386..6254)
                     /locus_tag="VFV55_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX4942807.1"
                     /translation="MKPFQMDSHALVALAHDAVALTLSWAFAFSLLGIRGDQGVPGEV
                     TVALLVAIPLQGLVSYLFGVYQGIWRYTSLPDVQRIVFSVLAGTVAVAILLNATGFGE
                     ALSYREYALYPLILVSIMSGSRMAVRSFKEWSLYGRSAEHIPVIVLGAGDAAVGLLKE
                     LSRSQEWRVVGLLDDDMQKRGRLLQGVRVMGPLDELPRLATRLKVRHAIIAMPSVPYK
                     VRRRVVEVCKRAKVTVLTVPSLDNVAGGKLAAPKIRKVAVEDLMKREPVTLDDAGLHD
                     LLTGKVVLVTGAGGSIGSELCRQIAAFQPSMLVLFEQNEYGMYRVDGELRDGFPEMPV
                     ASVVGDVTNARRVAQAMHQYSPSIVFHAAAYKHVPLMEELNSWEAVRNNVYGTYVVAR
                     AAIDYGVKKFVFVSTDKAVNPTNVMGATKRLAEMVCQVLQAGSATRFEMVRFGNVLGS
                     AGSVIPRFEEQIQRGGPVTVTHPEMVRYFMSIPEAAQLVLQAGCMGLGGEIFVMDMGE
                     PVKIVDLARDMIRLSDLSEEEIKIVYTGLRPGEKLFEELLADDEHTRPTPHPKLRVAK
                     AREAEPGAWLEELLVWLTQDRVSADAEVRRDLKRWVPEFQSQVRPRLKAVPGTRAA"
     gene            complement(6313..7302)
                     /locus_tag="VFV55_00545"
     CDS             complement(6313..7302)
                     /locus_tag="VFV55_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4942808.1"
                     /translation="MSGVFVVFLPAIATIVVIALLYVSPLAFRIVDHPGDRSLHATPT
                     PRIGGIGLMLGAMPVVFYFGAPDVRWIAGAAVVLAVVSFIDDVKSLPVAIRLPCHLAA
                     AALAVGVTWPGVGIGSAASVAIAAAALLAIAWMTNLFNFMDGADGLAGGMALIGFGAL
                     AVAALRGGDAALAYAALALSASAAGFLVFNIPPARVFMGDAGSVPLGFLAGALGWLGA
                     VRGVWPWWFPVLVFSPFIVDATVTLARRVAKGEPFLRAHRSHYYQRLVLGGWSHRRLA
                     AAAWVLMTAVAASATVALGKHVAVQGAIISLWAIAYGALMLWMDRRQPPDTRR"
     gene            complement(7299..8234)
                     /locus_tag="VFV55_00550"
     CDS             complement(7299..8234)
                     /locus_tag="VFV55_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX4942809.1"
                     /translation="MPDLFRLPLDPDFPVPRKALVTGAGGFVGSALVARLGPHAGLAL
                     GGDGWRDAIAGADFREATVVHLAARVHESRGEAAAFERDNTVKTRVLAEAAAAGGAAR
                     FVFASTVKVHGEETGAATFGPDSPPAPQDPYARSKWLAEEALRDIASRTGLPVVIVRI
                     PLTYGPGVGGNFRDLLRLADSATPLPLAAIRNRRSLVHVRDLVEALLLAAGHPKAPGR
                     AFIAAHPVPVSTPELVTALRSALGRPARLFPVPAFALEVAATVAGLRDRMRRLTRSLE
                     ADPATLMATLGWSPQVGLAAGVEDTVAAWREGAKG"
     gene            complement(8273..9631)
                     /locus_tag="VFV55_00555"
     CDS             complement(8273..9631)
                     /locus_tag="VFV55_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942810.1"
                     /translation="MNLEPLRRLQSKLSVAFVAATALVLTACGGGGATSSPNNVGNLQ
                     LLPASASIYAGVPYKANIVGGRKPYLVTSSEQTLIELNFTTDSNEFTFVARNPGVVDV
                     GLDPNEVPRRSVNIEIRDSNGATISNTYSVLQNFFTGYRESYSNTCPVATGDTAPQAC
                     AGIDSIVTLVPVSNGLLYSGREFQFDKVRGDFSFVVEDPAAVPQLVNQIRVRTDTNGK
                     AIVRLRPTATARTQLATYKVTDIATGVTTDVVFLIVGAQAQPLTLIPTSVTFTGGLTT
                     QCGGGSADIFIFGGTPPYTVAASNGISVSAVTPTIFGGRFTINVSGGGGGFSPIGTAV
                     CPTGLSVVVADSAGATAVLPVSSEPGTTTLPALTAVPSSIAVLGCGQSVGVTVVGGTG
                     SFTASSQHARVTAAISGNTLTLTRLTGDGATIYPVTGTASVTDGSTAISVTFSGMPNN
                     CP"
BASE COUNT         1667 a         3364 c         3368 g         1521 t
ORIGIN      
        1 agctcatcct cgacgcgatc gagcaccccg gccagggcct tcacgattcg cccgagcgcg
       61 aggtcgcggc aatccgtgag gacgcccgcg gattcatgcg gcgagagccg cgcgcgggcg
      121 cgcgcatgcg cgtcgaggga aacgacgttg ttccggggga tgctcgccat ggcgccgcct
      181 ttcgcgggcc gacccagggg ggaaggacgc tgtactggca atcccgctgt cgtaaggccg
      241 tgctgcgcga cctgttagac cggaaacttt gcgtccccac ctttcgatgg gtttgccctg
      301 tttcagaaag cgtgcgccgg gtgggttctc gacggcgcgc gcggaagagc gcgcgcaccg
      361 aaccattcta ggcagcgcca tgttggacgc agcttccgcc gccgtcaatg cccggccatc
      421 gggcaaaggc ggccgcgcat cgagcgaggt aagcgcttcc tcgccggtat aatcgaggct
      481 cctcccggcg agcacccgtg gcatgtcgat cctggtttgc ggctccatcg cgtacgacac
      541 catcatggtg ttccgcgacc aattcaagaa tcacatcctt cccgaccaga tccacatcct
      601 gaacgtcgcc ttcctggtgc ctgacatgcg ccgcgaatac ggcggctgcg cgggcaacat
      661 cgcctacggc ctgaaactcc tcggcgagga gcctttcatc atggccaccg tggggcacga
      721 cttcgagccc tacgggcgcc gcctggacac gctggggctt tcgaaaaggc acgtgcgcct
      781 gctcgacgag caattcaccg cgcaggcctt catcaccacc gacatcgacg acaaccagat
      841 caccgccttc cacccggggg cgatggcgca atcgcacctg aaccgcattg ccgacacgag
      901 cggcatgcgc ctcgccatcg tctcccccga cgggcgccag ggcatggtgg aacatgcccg
      961 cgaccttgcg cgggcaagga tcccgtatgt actcgacccg ggccagggcc tgcccatgtt
     1021 ctcgggcgac gacgtcctgg agatgctcgc cggggcgcgc tgcctcacgg tgaacgacta
     1081 cgaggcgcgc ctggtcgaac agaagaccgg caagacgatc gccgaactgg cgacgcgggt
     1141 cgaggcggtg gtggtgaccc gcggggccga gggagcggac atcctctccg gcggtcaaca
     1201 gattcacgtg ccggccgtta aaccggatag cctggtcgat ccgaccggtt gtggcgacgc
     1261 tttccgcgcg ggcctcctct acgggatggc ccgcggctgg gattggcgca ggtgcgggcg
     1321 gctggcatcc ctgatgggct cgatcaagat cgcgtaccgg ggaggccaga accaccgggt
     1381 ttcccgtgac gagatcgaaa ggcgattcgg ggacgcgttc ggcgaaaggc tctagatccc
     1441 gccccgttgg gggccgggca ttcgaaaacc aaggagaaaa gcgatgcaag caagatcact
     1501 gggaaccgct ctcgtggcca ccgccctcct gggcgtggcg ggctgcgcag ctcccccgcg
     1561 cggcggcgcc gactatcgcg gctacgagac ccgtacggag cagtcggtgc gcttcggcgt
     1621 ggtcgagtcg gtgcgcggcg tgcgaatcaa cccgaccgag acgggcgtcg gcacggcgtc
     1681 cggggcagtg ctgggcggca tcgcgggaag cacgatcggc catgggcgcg gttcggcggc
     1741 cggcgcagtg gccggcgcgg tcctcggcgg cgtcatcggc tcggcggtgg aaaaggacgc
     1801 caacgatcgc cagggcatcg agatcaccgt catgctggac ggcggcaagt acatcgccgt
     1861 ggtgcaggaa gccgacgagt ccttccgggt cggcgaccgc gtgcgcatcc tgtccggccg
     1921 cggcaccacc cgcgtcacgc actgagggcg ccccacccga cccccaggct gctgcgcgcc
     1981 gcgcggtggg cgcggctgct cgttcacctg ggctccgcct tcatcatgct gcgcgtcgcg
     2041 tggccgcgcg ccagtgcgca gcgccgccac gagatcgccg tgcaatggtc gcaggacctc
     2101 cttgccattc tcgccgtgcg cgttcactgc gacggcaagc ccccgtcgca ggggcgaacc
     2161 ggcgccatga tcgccgccaa ccacgtatcc tgggtcgaca tcttcgccat cgcctcggtt
     2221 cggcacacgc gtttcatcgc gaagagcgag atccgggatt ggccgctggc gggctggctc
     2281 gcggcgaagt ccggcacgat cttcatccgg cgcgcgcgcc gccatgacac ggcgcgcatc
     2341 aacgcgcagg tgcacgatgc gctcgccgaa ggcgaatgcg tggcgctctt ccccgaggga
     2401 acgacaaccg agggcgaccg gctcctcaag ttccacagct cgctcttcga gccggcggtg
     2461 gccaacggcg cgcgcgtcta tccggtggcc gtccgctacg aagatcgcga cggaacgccg
     2521 cttcgctctg cggcctacgt gggggatctg actttcgccc agtcgctcgg gctcgtcatc
     2581 cgcacgcgcg agacgatcgc gcgccttgcc ttcgcggagc caatccaccc cgagcgcctg
     2641 tcgcggcagg acgtggcgat ggaagcgcac cggcgggtgg ctactctgct gggtctcccg
     2701 tgcgcgggca cggcacctgc caaagccgcc gatcgcccag gcgaaccgcg gtaagggcgt
     2761 tcccccatac gcaaccgctg tccagggaaa gcaccttgtc gccgacgtgc agtcccagcg
     2821 ccgaccagtg cccgcagacg atcgtatggg tggcgttcgc ccgccccggc atgtcgaacc
     2881 acgggatgcg gtgcgagtgc ctctcccccg gctcgccctt gaaggcgagg tccatcgtac
     2941 cctccggggt gcatacgcga agccgggtca tcgcgttaac gatggcacgc aggcggtcct
     3001 cgcctgccaa cgcatcgttc cagcgcgcgg gctcgttgcc gtacaggcgc gcgagaaagc
     3061 gccgccaccc cggcccccgg agcaccgcct cgacctcgcc ggcgagctcc ctggccttcg
     3121 cgaccggcca ttgcggcagc aaccccgcgt gcaccatcgc gaatgcgccc tcgacgtgca
     3181 tgagcggaag gttgcgcagc cagtcgacga gctcgtcccg gtccggtgcg gcgagcacgc
     3241 cctcgagggt gtcgcgcggc cgcggcttct cgatcccctc cgccacgcag agcaggtgca
     3301 ggtcgtggtt gcccagcacc gtgatcgcgg caggccccag gctcttcacg aaacgcaggc
     3361 aacccagcga atcggggccg cggttcacca ggtcgcccac gaaccacagg cggtcgcggt
     3421 ccgggtcgaa acggatcgcc tcgacgaggc gctcgagggg ctcgaggcac ccttgcaggt
     3481 caccgatcgc gtagagggcc atggagaagg ctggatgcga aaaagggcga ccgaaatcgc
     3541 cctttcgcgt cgcgcgggcg gatcgcggct acttcgagcc gccgaccatg tagtcgaccg
     3601 cggctttcac gtccgcatcg ggaagggcgg cattgccgcc tttcggcggc atggcggcct
     3661 tgcccttgag cgcgctcccg tagagcgcat cgagccccgt tgcgatacgc ggggcccagg
     3721 cggccttgtc gcccaatttc ggcgcaccgg ccacgccggt cgagtggcag gccatgcagg
     3781 tcgtgtcgta gaccttcttg ccatcggcct tggcggcctt ggctgctgcc gccggggcgg
     3841 ccggggcagc aacggtggct actttgggac cttgagtgac gggtgccccg gcaggcgccg
     3901 cggcaggtgc ccccgcaggc gccgcggcag cggcagtctc gacctgcagc ctggcaacgg
     3961 gcgcgatgcg cttggcaagc gcttccctgg ccatggccgg gtcgttttcg agcgatcgcc
     4021 cgccatagac gccgatcgcg aactgcgcca gcaggatgat gcccacgatg agtgccaccg
     4081 cgccgatgac gatccagatg gcgagcttca ggggatgcga atcgggattc gccttgacgg
     4141 cgtcggaatg gtaggcctgg gacatggttt cctcgggaat cggctgcggt catgccgcga
     4201 tcggaaggcc gcgattatag cccgcccgcc ttgcgcagat cacggtcccc gttggacggg
     4261 gggtgtcgaa gtcgtctaga atccggcacc tacgcgcccg tagctcagct ggatagagta
     4321 ctgccctccg aaggcagggg tcgcacgttc gaatcgtgtc gggcgcgcca accgattccc
     4381 ccccgctacg ccgcccgcgt ccccggcacc gccttgagcc tgggccgcac ctgcgactgg
     4441 aattccggca cccaccgctt gaggtcgcgg cgcacttcgg cgtccgccga gacccggtcc
     4501 tgcgtaagcc acacgagcag ttcctccagc catgcgccgg gttccgcctc ccgcgccttg
     4561 gcaacgcgaa gcttcgggtg cggcgtgggc cgcgtgtgct cgtcatccgc gagcagttcc
     4621 tcgaagagct tctcgccggg ccgcaggccc gtatagacga tcttgatctc ctcctcggaa
     4681 agatcggaga ggcggatcat gtcgcgcgca aggtctacga tcttcaccgg ttcgcccatg
     4741 tccatcacga agatctcgcc gcccaggccc atgcacccgg cctgcagcac gagctgcgcg
     4801 gcttccggga tggacatgaa gtagcgaacc atctccggat gggtgacggt gaccgggccg
     4861 cccctctgga tctgctcctc gaagcgcggg atgacgctgc cggcgcttcc gagcacgttg
     4921 ccgaagcgca ccatctcgaa gcgcgtggcg ctgcctgcct gcaggacctg gcacaccatc
     4981 tcggcaagcc gcttggtggc acccatcacg ttggtgggat tgaccgcctt gtccgtggag
     5041 acgaagacga acttcttcac gccgtagtcg atggccgcac gtgccaccac gtaggtgcca
     5101 tagacgttgt tgcgcaccgc ctcccaggag ttgagctcct ccatcagcgg cacgtgcttg
     5161 taggccgcgg catggaagac gatcgacggc gagtactggt gcatcgcctg cgccacgcgc
     5221 cgggcgttcg tcacgtcgcc gacgacgctc gccaccggca tttccgggaa gccgtcgcgc
     5281 agctcgccgt cgacgcggta catgccgtac tcgttctgct cgaagagcac gagcatcgag
     5341 ggctggaagg ccgcgatctg gcggcagagc tcggagccga tggagccccc cgcccccgtg
     5401 accaggacca ccttgccggt gagcaggtcg tgcaggcccg cgtcgtcgag cgtcaccggt
     5461 tcgcgcttca tcaggtcctc gaccgccacc ttgcggatct tgggggccgc gagcttgccg
     5521 ccggcgacgt tgtcgagcga gggcacggtg agcacggtga ccttggcgcg cttgcaaacc
     5581 tccaccacgc gccggcgaac cttgtacggc accgagggca tggcgatgat cgcgtggcgc
     5641 accttcagcc gcgtggccag gcgcggcagt tcgtccagcg gccccatcac gcgcacgccc
     5701 tgcagcaggc ggccgcgctt ctgcatgtcg tcgtcgagaa ggccgaccac gcgccattcc
     5761 tggctgcgcg agagttcctt gagcagcccc actgccgcat cgcccgctcc cagcacgatc
     5821 accgggatgt gctcggcgga gcggccgtag aggctccatt ccttgaacga gcgcaccgcc
     5881 atgcgcgaac cggacatgat cgacacgagg atgagcgggt agagcgcgta ttcgcggtag
     5941 ctcagcgcct cgccgaaacc cgtcgcgttc agcaggattg ccaccgcgac ggtgcccgcg
     6001 agcacggaga acacgatgcg ctgcacgtcg ggaaggctgg tgtagcgcca gatgccctgg
     6061 tacacgccga agaggtagct cacgaggccc tgcagcggaa tggccacgag cagtgcgacg
     6121 gtgacctctc ccgggacgcc ctgatcgccg cgaatgccca gcagcgaaaa ggcgaaggcc
     6181 cagctgagcg tcaacgccac cgcgtcgtgg gcgagcgcga cgagggcatg cgagtccatc
     6241 tggaatggtt tcatggcgtc aggtcgtgag aaattgctta tcgaagcgcg ggtgcgcgat
     6301 tatttttctc tctcaccgcc gtgtgtccgg cggttgcctg cggtccatcc acaacatgag
     6361 cgcgccgtag gcaatcgccc aaagggaaat tatagcgcct tgcaccgcaa catgctttcc
     6421 cagggcgacc gtcgcgctcg ctgccacggc cgtcatcagg acccacgccg cggccgccag
     6481 gcgccggtga ctccagccgc cgaggaccag ccgctggtag taatggctgc ggtgcgcccg
     6541 caggaacggc tcacccttcg ccacccgcct ggcgagcgtc accgtcgcgt ccacgatgaa
     6601 tggcgagaag accagcaccg ggaaccacca cggccacacg ccgcgtaccg cgcccagcca
     6661 accgagcgcg ccggctagga acccgagcgg gacggagccc gcatccccca tgaacacgcg
     6721 cgcgggcggg atgttgaaca cgaggaagcc cgccgcggag gcgctgagcg caagcgcggc
     6781 gtacgcaagc gcggcatcgc caccgcgaag cgcagcgacc gcgagagccc cgaatccgat
     6841 gagggccatg ccgccggcca atccgtcggc gccgtccatg aaattgaaga gattcgtcat
     6901 ccacgcgatg gcaaggaggg cggcggcggc gatcgctacg ctggccgcgc ttccgatccc
     6961 cacgcccggc caggtgacgc ctacggccag ggcggccgcc gcaaggtgac agggaagccg
     7021 gatcgccacc ggcaagctct tcacgtcgtc gatgaaggac accactgcga gcactacggc
     7081 ggcgcctgca atccagcgca cgtcgggcgc gccgaagtag aacacgaccg gcatcgcgcc
     7141 gagcatgagg ccgataccgc cgatccgggg cgttggcgtt gcgtgcaggg accggtcgcc
     7201 ggggtgatcg acgatccgga aagcgagtgg gctcacgtac aacagggcga tcaccacgat
     7261 ggtggcgatc gccggcagga agacgacgaa gacgccgctc atcccttggc gccttcgcgc
     7321 cacgcggcga cggtgtcttc cacgccggcg gcgaggccaa cctgcggcga ccaaccgagt
     7381 gtcgccatga gcgtggcagg atcggcctcg agcgagcggg taaggcggcg catcctgtcg
     7441 cgcaggccag caacggttgc tgccacttcg agcgcgaatg ccggaaccgg aaagagtcgc
     7501 gcaggacggc ccagcgccga gcgcagcgcc gtcaccagtt ccggcgtcga taccggaacg
     7561 ggatgggcgg cgatgaaagc ccggcccggt gccttcggat ggccggcagc cagcagcagg
     7621 gcttcgacga gatcgcgcac gtgaacgaga ctgcgacggt tgcggatcgc ggccagcgga
     7681 agcggcgtgg cggaatccgc gagccgcagg aggtcgcgga agttgccacc cacgccgggt
     7741 ccgtaggtga gcgggatgcg cacgatgacc accggcaatc ccgttcgcga ggcgatatcg
     7801 cgaagcgctt cctcggcaag ccatttcgac cgggcatagg ggtcttgcgg ggcgggcggc
     7861 gaatcggggc cgaatgtcgc cgcaccggtt tcttcgccgt ggaccttgac cgtgctcgcg
     7921 aacacgaagc gcgcggctcc cccggcagcg gccgcctcgg cgaggacccg cgtcttcacg
     7981 gtattgtcgc gctcgaaggc cgccgcctcg ccccgcgact catgcacgcg cgccgcaagg
     8041 tgcaccaccg tggcctcccg gaaatcggcg ccggcaatgg cgtcgcgcca gccgtcacca
     8101 cccagggcga gccccgcatg cgggccgagt ctcgccacga gcgcggaacc cacgaacccg
     8161 ccggcgccgg tgacgagcgc tttacgcggg acgggaaagt caggatccaa cgggagccga
     8221 aacaaatcgg gcatcccgaa agatgcccga tttcgcgagc cttgccgcta cgttatgggc
     8281 aattgttcgg catcccggag aaggtgaccg agatggcggt gcttccgtcc gtgacgctcg
     8341 ctgtgcccgt caccgggtat atcgtcgcac cgtcgccagt caggcgggtc agcgtgagcg
     8401 tattgcccga gatggccgcc gtcacgcgcg cgtgctgcga gctcgccgta aaggagccgg
     8461 tgccgccgac gaccgtcaca cccacggatt ggccgcaacc gaggactgca atgctcgagg
     8521 gcacggccgt aagcgcgggc aaggtggttg taccaggttc gctgcttacc ggcagcaccg
     8581 ccgtcgcacc cgcgctgtcg gcaaccacca ccgacaagcc ggtcgggcaa accgcagtcc
     8641 cgatcggcga gaatcctccc cctcctcccg aaacgttgat cgtgaatctg ccgccgaaaa
     8701 tggtgggagt gaccgcagag accgaaatgc cgttcgaggc tgcgacagtg tagggcggcg
     8761 ttccgccgaa gatgaagata tcggccgatc ctcctccgca ctgggtcgtg aggcccccgg
     8821 tgaaggtgac ggaggtggga atgagcgtga gcggctgggc ctgggcaccg acgatgagga
     8881 agacgacatc cgtcgtgacg ccggtcgcga tgtccgtcac cttgtacgtg gcgagctgcg
     8941 tccgggcggt ggccgtcggg cgaaggcgaa cgatggcctt tccgttggtg tcggtcctga
     9001 cgcggatctg gttcacgagc tgcgggacgg ccgcgggatc ctcgacgacg aacgagaaat
     9061 cgccgcgcac cttgtcgaac tggaactccc ggccgctgta caggaggccg ttggacacgg
     9121 gcaccagggt gacgatggag tcgatgccgg cgcaggcctg tggcgcggtg tccccggtgg
     9181 cgaccgggca ggtattcgaa tagctctcgc ggtaacccgt gaagaagttc tgcaggacgc
     9241 tgtaggtgtt gctgatcgtc gcgccgttgc tgtcgcggat ctcgatgttc accgagcggc
     9301 gcggcacttc gttcgggtcg aggcccacgt cgaccacgcc cggattgcgg gcgacgaacg
     9361 tgaactcgtt gctgtcggtg gtgaagttga gctcgatgag cgtctgctcg ctcgaggtca
     9421 ccaggtaggg cttgcgcccc cccacgatgt tggccttgta gggcacgccc gcgtagatcg
     9481 acgcgctggc aggcaggagc tggaggttgc cgacgttgtt cggcgaggag gtggccccac
     9541 cgccgccaca ggcggtcagg acaagcgcgg tcgcagccac gaaagcgacg gacagcttcg
     9601 attgcaggcg acgcagcggc tcaagattca cgattgactc ccttttttgc ggggtcccgc
     9661 acggagattt cttcaaaatt agcgggttat cgacgtttat taaagcgcaa cttgcagttg
     9721 cttgcaagca gaagttttgc ccgatgcaac cggcgagtca cgccattgcc gcggggcaac
     9781 aattgcgggt ccgatcacct ttgggcacat gcgccaacga tctaccggca atgagtcggt
     9841 gctctggcca atgcggtcgc tgtcgttcgt ggcagtgaac tagccgcagg tcgttccgtt
     9901 caccggaatc aggattgacg
//
LOCUS       DASUEV010000022        13794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_808580, whole genome shotgun sequence.
ACCESSION   DASUEV010000022 DASUEV010000000
VERSION     DASUEV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808580"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..2083
                     /gene="uvrC"
                     /locus_tag="VFV55_00560"
     CDS             284..2083
                     /gene="uvrC"
                     /locus_tag="VFV55_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX4942811.1"
                     /translation="MFDPKEFLAGLPNLPGVYRFLNAAGEVIYVGKARELKKRVSSYF
                     QKSHPSPRTTLMVGQIAAAETTVTRTEAEALLLENNLIKALSPRYNVVFRDDKSYGYI
                     LVTGHRFAQIRFYRGVQEKPNRYFGPFPSAWAVRETIGHLQKIFRLRTCDDSVFSHRS
                     RPCLLAQIGRCSAPCVGRIAEEDYRRDVDHAAKFLEGREDDVIAELNQKMEAASGAQD
                     YELAARFRDEIRMLQRILSGQAVDSAGARDADVVAAVEKEGLWCVTLAMIRGGRHLGD
                     RSFFPQNAGGSDAATVVEAFLTQHYAVQPVPPRVIADSVEDAPALAAFLEAIANRAVT
                     VLTRPQGEARLWLEMARKNAELAVASRLAAQATQEARGQALTEFLGAESPVARIECFD
                     ISHTMGEATVASCVVYDKGGMQAAEYRRFNVKDVAAGDDYGAMRYALEARYRKLADGE
                     GKTPDLILIDGGTGQLNAAVGMMQDLGLAGITMIGVAKGEERKPGLEQLFVAGEEGAR
                     RLPPDHPALHLIQQVRDEAHRFAITGHRARRGKARTASRLEDIGSIGPKRRQKLLAHF
                     GGLQGVITASVEDLARVQGISRTLAERIYNELH"
     gene            2097..2681
                     /gene="pgsA"
                     /locus_tag="VFV55_00565"
     CDS             2097..2681
                     /gene="pgsA"
                     /locus_tag="VFV55_00565"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX4942812.1"
                     /translation="MPLNLPLVLTWLRILAIPLFVAVLYFPEDWLSERQANVISMWIF
                     IVAAITDWADGYLARRWNQTTSFGAFLDPVADKLMVTAALIVLVEKGFVDPFAALIII
                     GREITISALREWMAQIGQSKSVAVNILGKVKTVTQLVAIPFLLFNGVLFGLFHTNPVG
                     RVLVWVAALITLWSMVVYLKAAMKPAGAGGGTQA"
     gene            2721..2796
                     /locus_tag="VFV55_00570"
     tRNA            2721..2796
                     /locus_tag="VFV55_00570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2754..2756,aa:Gly,seq:gcc)
     gene            2876..2949
                     /locus_tag="VFV55_00575"
     tRNA            2876..2949
                     /locus_tag="VFV55_00575"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2908..2910,aa:Cys,seq:gca)
     gene            complement(2977..6600)
                     /locus_tag="VFV55_00580"
     CDS             complement(2977..6600)
                     /locus_tag="VFV55_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX4942813.1"
                     /translation="MIPTAWRRPATLVVAVAGLVALYAAVGFFVVPSVARSKIELLAR
                     TELGRQATIGKVEFNPFTLRARLSDFTLTDRDPGRTLLKFEALVVDVSAESLWRRAPV
                     LDAVRLVQPRVEIARSADGRFGIQDLIERALAPSREPESRFAVNNIEIEDGSILLDDA
                     VHQRRIAVTKLEIGLPFLSSLSRDAQIRVTPRLHGAVDGAPFGLTGTATSPFEDLQRA
                     TLELNLDALPLPRYLAYAPLPSGLNLTDGALTTRLSLAFVTSKGTPRGITLSGNARVD
                     RLAIARREATPLIAARSIAVAIGNLDFLRRSIAVDRIAVDGPMLDLRRLADGTLEIQK
                     LLAVTPESAAADTPGTSATRAVPAPNDAARPTWAWSVTETRVSGGDLSIADEAVAPPF
                     KTTLSAITLTGAKLASQGAPGTLDVAFDSEDAHFAAHGVVDFAGNAARGHFDLTKLAL
                     ARLRPYSTSVLAMDVKRGALDFGADFDAGTSPATRFTLAQGSAVLADLDVALRSEREP
                     LWRMARASADGIALDLAARTATIERIEAQKAAIRLLRDADGRMHFERAMRTAPGRANP
                     PDAAETAKEKAGDTDWHVVVRQLLLERFGVDLEDRVPDPAVKLRVADAKVVAENLDTA
                     RGSKAKIDVTARVGAKGRVRLNGVVSARPASADCRIDATALDLVPLRSYFESRTNVIL
                     TSGAVDAKGRITYGEAGTSGPQGRFVGDVIVNDFDSLDRPGSEELVRWKSLGLTGIDV
                     TSAPFNLALNDVALDGFYARLILNADATLNVQRLLAPDASEKASATPAQQGSVIAAAD
                     APPPAPAGERREIPASIGRIRLSNGEVEYSDFFVKPNYSAHLTDVTGSVSALAAAQSG
                     TVELTAKIDRSAPVDIRGTVNPFAKELSLDLTAKATGVDLPSLTPYSVKYAGYGIQKG
                     KLSMEVHYKLDNRKLSANNKLMLDQLIFGEHVDSPTATKLPVLLAVALLKDRNGMINL
                     ELPIEGTLDDPQFSVWRVVVQIFVNLLTKAVTAPFALLGAIGGGGGEQLAYVEFAPGS
                     ADLSAAALAKLTTLAKALTDRPGLKLDAAGRAIPDIDREALKRATLNHAMRIRKQKEL
                     ASGGESAPALDALTIDAADRAKYLKAVYRDADLKDKPRNFLGIAKDVPPEQMEAMLLA
                     SYRIDDEALRELANHRANVVKEWFGRKGNVAPERVFVIAPRLDAEGIKADGAPTRVDF
                     AIR"
     gene            complement(6597..7652)
                     /locus_tag="VFV55_00585"
     CDS             complement(6597..7652)
                     /locus_tag="VFV55_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4942814.1"
                     /translation="MYRHILVALDDTDLATQTVTTSIAFARALCARITFFYATPEYAG
                     AGNGAPNHSMGNMTHSEAELGQTNAILSKALTAAAALGMKCGAVAATSGDPPLAIVEA
                     ARREGCDLIFVSSHGPQRGVRGWFTTNNTQKLLQISDLPVHVATVQANQRDADENRAL
                     AVIHGEHRSIGTALRGLEHLVSQGRAQGNRLDVSLLRLIVEYMFAFPEALHHPKEETH
                     LFRLLGLRAPELNDTLEELVRQHGRERSLVEALGAALDRFESNEPDAFEGVASAVSTL
                     TDTVWEHMGLEENTVLPAASKCLGPEEWKQIADSFEANAEPLKHRGFSERAFAALFTR
                     IANRLPGVAANRGSTGA"
     gene            complement(7711..8400)
                     /locus_tag="VFV55_00590"
     CDS             complement(7711..8400)
                     /locus_tag="VFV55_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4942815.1"
                     /translation="MTAAKLLPATSAQGAADALEEDIVLGRLHPRQRITEDELLERFG
                     LKRHVVREVLATLERMGLVERRRNIGALVRSFTPREVRELYAMRELLETEAARRMPLP
                     PPAAALDRLAAIQRDHDKAVARRNLREVHRTNLAFHRELFALAGNEVLENAITEYARQ
                     THAIRFSALVSPDFREQAREEHQAMIRALAAKDRVHLVALCRRHLRPARDAYLEHLAP
                     DPGGGGLIART"
     gene            complement(8414..10141)
                     /locus_tag="VFV55_00595"
     CDS             complement(8414..10141)
                     /locus_tag="VFV55_00595"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX4942816.1"
                     /translation="MTDPSSKAAKPPAAGLSKGLTSYGDTDFSLFLRKAFIKGAGFTD
                     EALSRPVIGIANTASGFNPCHGNAAQLLEAVNRGVMLAGGLPVAFPTISIHESFAYPT
                     SMFLRNLMSMDTEEMIRAQPMDAVVLVGGCDKTVPAQLMGACSAGVPAIQLVTGSMLT
                     GSHDGERVGACTDCRRYWGRYRAEEIDAAEISRVNNQLVASVGTCSVMGTASTMACVA
                     EALGIALPGSASPPAVTADRIRIAERSGSTAVALAKARLTPGRIVTAKSVENALRVLL
                     AIGGSTNGIVHITAIAGRLGIPVDLARLDAIGRETPVLVDLKPSGSHYMEDFHHAGGV
                     PALLRELKSLLNLDALTVTGRTIGEEIEASGPGFRQDVIRPLANPIFAQGGIAVLRGN
                     LAPGGAIIKQSAADPKLMQHEGRAVVFENAEDMARRIDSDDLDVTAGDILVLKNIGPK
                     GAPGMPEAGYLPIPRKLARAGVKDMVRISDGRMSGTAAGTIVLHVTPESALGGPLAYV
                     RNGDRVRLDVPARSVALLVTDAELCARRAANPVTPPSAERGYLKLFLDCVTQADEGVD
                     FDFLRAPRK"
     gene            10259..11251
                     /locus_tag="VFV55_00600"
     CDS             10259..11251
                     /locus_tag="VFV55_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4942817.1"
                     /translation="MTSPFLRLFAKRAASLIAVTLLFAAGGAMAQYPNKSIKLIVPYP
                     PGGATDVIGRVLAQRLSTALGQQVIIDNRGGAGGNIGAEAVAKSAPDGYTLLMGALTS
                     HSIMATLEKGTLRYDLARDLVPVAVVGSVPLVWVVYPPLPVNSLKELVAYAKANPGKL
                     TYASSGAGAPQRMCAELFRLKIGADMIHVPYKGSGPAMGDLVGGQVVTMCETVPASLQ
                     LIKAGKLRALAVTTAQRISQLPDVPTAAEAGTPGIEVSSLFGVMAPAGTPAPIIARLN
                     AELQKILATPEAQEQLLQQGAYAVQMSPEQSAARIRQEIDLWARVIQEAKIKSD"
     gene            complement(11296..12159)
                     /locus_tag="VFV55_00605"
     CDS             complement(11296..12159)
                     /locus_tag="VFV55_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4942818.1"
                     /translation="MQPATVMVAPGIYVLPGSGGEITRKNGGRTANVAFVVGPRGVIV
                     VDTGISHRQGGDIIAAVESISNRPIRLAILSHPGQESIFGAAAFQERGIPVLAHRSGA
                     KLMAARCETCLRRLRPLLGEEAMAATRVVTPDRLIDGDETLDLIGRRLRLIAPRWSSV
                     PGAIAVFDETTSTLIAGNLVAIERVPDMRDANPAAWRRTLAQFASMRCRHLVPGYGPP
                     GTCADIPAFDRYFAALEGRVETLLRDGVSLAELRHRCDLPEFAHWDQYEALHPQNASH
                     AYLRLERSQFE"
     gene            12140..12451
                     /locus_tag="VFV55_00610"
     CDS             12140..12451
                     /locus_tag="VFV55_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942819.1"
                     /translation="MTVAGCTERVPGVAQDTTRMPQSAAMNAACIAASSLRRHAATRG
                     DDGRAMLLQCIVSKSPRQRRTSAPEGHGLSLILLRGNAAGQHDIPVDAAQRAGNFLQT
                     N"
     gene            12587..>13794
                     /locus_tag="VFV55_00615"
     CDS             12587..>13794
                     /locus_tag="VFV55_00615"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX4942820.1"
                     /translation="MTSASTRLAVAISGFALAAPGIAAANADLEKQIADPNNWAMQAG
                     DMYNQRYSKLTQINAQNVGKMQVAWMFSTGVLRGHEGSPLVANGMMYVHTPFPNKVFA
                     IDLDTQKIKWRYEPKQDVAVIPQMCCDTVNRGVAYAEKKIFLQQADSTLVALDANNGK
                     VIWSVKNGDPKAGAVNTNAPHVFKDKVITGISGGEWGVRGFLAAYDINTGKQVWKAYS
                     TGPDNEMLIDPDKTMTWTDGALKPVGKDSSLKTWKGDQWKTGGGTTWGWYSYDKALNL
                     VYYGTGNPSTWNPAQRPGDNKWSMTVFARDVDTGVAKWAYQMTPYDEWDFDGINEMIL
                     ADINVKGKATKALVHFDRNGFAYTLDRATGALLVAEKYDPTVNWATHVDMKTGRPQVV
                     KQYSTQAQGQ"
BASE COUNT         2281 a         4572 c         4714 g         2227 t
ORIGIN      
        1 ggacacgctg ctcgagggac tggagcgccg cccgcttgcg cccaccctcg cgcgccgcat
       61 cgggaccatg cgtggccgcg ccatctcccc cgccgccctc gaggcgagtg ccgcctacct
      121 tgccgccacc gagaccctgg cgcgcgttcg cgcataaagg ggtcaggtta ctttgcagaa
      181 aggggtcagg ttactttacc gcccatgatt cccggcattt gccgggtgat tcgtaaagta
      241 acctgacccc tttctgcaaa gtagcctgat ccctcctacg cccatgttcg acccgaagga
      301 attcctcgcc gggctgccca acctccccgg cgtctaccgg ttcctcaatg cggccggcga
      361 ggtgatctac gtcggcaagg cgcgcgagct gaagaagcgc gtctcgtcgt atttccagaa
      421 gagtcacccg agcccgcgca ccaccctcat ggtggggcag atcgccgcgg cggaaaccac
      481 cgtcacgcgc accgaggcgg aggcgctcct tctcgaaaac aacctcatca aggcgctcag
      541 cccgcgctac aacgtcgtct tccgcgatga caagagctac ggctacatcc tcgtcaccgg
      601 gcaccgcttc gcgcagatac gcttctatcg cggcgtgcag gagaagccca accgctactt
      661 cggcccgttc ccgagcgcct gggcggtgcg cgaaaccatc ggccacctgc agaagatctt
      721 ccgcctgcgc acctgcgacg actccgtgtt cagccaccgc tcgcgaccgt gcctgctggc
      781 gcagatcggc cgctgctcgg cgccgtgcgt gggcaggatc gccgaggagg actatcgccg
      841 agacgtggac cacgccgcca agttcctcga gggccgcgag gacgacgtga tcgcggaact
      901 caaccagaag atggaggcgg cttccggcgc gcaggattac gagctcgccg cccgcttccg
      961 cgacgagatc cgcatgctcc agcgcatcct gtccggccag gcggtggatt cggccggggc
     1021 gcgcgacgcc gacgtggtgg cggctgtgga aaaggagggc ctgtggtgcg tgacgctcgc
     1081 catgatccgc ggcggccggc acctgggcga ccgcagcttc tttccgcaga atgccggcgg
     1141 cagcgatgcg gcgaccgtgg tggaggcttt cctcacccag cactatgccg tgcagccggt
     1201 gcccccgcgc gtgatcgcgg attcggtgga agacgcaccc gcgctcgcgg cattcctaga
     1261 ggccatcgcg aacagggcgg tgaccgtgct cacgcggccg cagggcgagg cgcggctctg
     1321 gctggagatg gccaggaaga acgccgagct cgcggtggcg agccgccttg ccgcgcaggc
     1381 cacgcaggag gcgaggggac aggcgcttac cgagttcctc ggggcggagt cgccggttgc
     1441 gcgaatcgag tgcttcgaca tcagccacac gatgggcgag gccacggtcg cctcgtgcgt
     1501 cgtctacgac aagggcggca tgcaggccgc ggaataccgc cgcttcaacg tgaaggacgt
     1561 ggcggccggc gacgactacg gcgccatgcg ctatgccctg gaagcgcgct accgcaagct
     1621 cgccgacggc gagggaaaga cgcccgatct catcctcatc gacggaggca ccgggcagct
     1681 caacgccgcc gtcggcatga tgcaggacct cggcctcgcg gggatcacca tgatcggcgt
     1741 cgccaagggc gaggagcgca agcccgggct ggagcagctc ttcgtggcgg gcgaggaggg
     1801 cgcgaggagg cttccccccg accatccggc gctgcacctc atccagcagg tgcgcgacga
     1861 ggcgcaccgc ttcgccatca ccggccaccg cgcgcggcgc ggcaaggcgc gcaccgcctc
     1921 gcggctcgag gacatcggct ccatcgggcc gaagcgccgc cagaagctcc ttgcgcactt
     1981 cgggggactg cagggcgtga tcacggcgag cgtggaggat cttgcgcgcg tgcaggggat
     2041 cagccgtaca ctcgcggaac ggatctacaa cgaactgcac tgagcgcgaa actgccgtgc
     2101 ccctgaacct cccgctggtc ctcacgtggc tgcgcatcct cgccatcccg ctcttcgtgg
     2161 cggtgctcta cttcccggag gactggcttt cggagcggca ggcgaacgtg atctccatgt
     2221 ggatcttcat cgtggccgcc atcaccgact gggccgacgg ctacctcgcg cgccggtgga
     2281 accagacgac atccttcggc gcctttctcg acccggttgc cgacaagctg atggtcaccg
     2341 ccgcgctcat cgtcctggtc gagaagggct tcgtggaccc gttcgccgcc ctgatcatca
     2401 tcgggcgcga gatcaccatc tcggcgctgc gcgagtggat ggcgcagatc gggcagtcga
     2461 agagcgtggc ggtgaacatc ctgggcaagg tgaagaccgt cacgcagctg gtcgcgatcc
     2521 cgttcctgct cttcaacggc gtgctcttcg ggctcttcca caccaatccc gtgggacgcg
     2581 tgctggtctg ggtggccgcg ctcatcacgc tgtggtcgat ggtggtgtac ctgaaggcgg
     2641 cgatgaagcc ggccggggcc gggggcggaa cgcaggcttg aacgcagggc ccggaaaacg
     2701 ctataatgcg cggcttccat gcggcagtag ctcagttggt agagcgcaac cttgccaagg
     2761 ttgaggtcga gagttcgaga ctcttctgcc gctccaattt cccttcgggg ggaggtccat
     2821 cctccccccg catcgtttca aggtcccggt tgcgccgatg ccgccgggca ccccgggcgg
     2881 ggtggcagag tggtcatgca gcggcctgca aagccgtgta cagcggttcg attccgctcc
     2941 ctgcctccaa ctgatgcaat ccggcgattc ggcaactcac cggatcgcaa aatccacgcg
     3001 cgtcggcgcc ccatccgcct tgatgccctc ggcgtcgagc cggggcgcga tgacgaacac
     3061 ccgctccggc gcgacgttgc ccttgcggcc gaaccactcc ttgacgacgt tcgcacggtg
     3121 gttcgcaagc tcccgcagcg cctcgtcgtc gatgcgatag ctcgcgagga gcatggcctc
     3181 catctgttcc ggcggaacgt ccttggcgat gcccaggaag ttgcgcggct tgtctttcag
     3241 gtccgcatcg cgatagaccg ccttcaggta cttcgcgcga tcggccgcgt cgatggtgag
     3301 ggcatcgagc gcaggggcgg attcaccgcc ggatgcaagc tccttctgct tccggatgcg
     3361 cattgcgtga ttgagcgtgg cgcgcttgag ggcttcacgg tcgatgtccg gaatcgccct
     3421 tcccgccgca tcgagcttga gccccggccg gtcggtgagc gccttggcaa gcgtcgtcag
     3481 cttggccagc gcggcagccg acaggtccgc gcttcccggc gcgaattcga catacgccag
     3541 ttgttcgcca ccgccgccac cgatcgcccc gagcagcgcg aacggcgcgg tcaccgcctt
     3601 ggtgaggaga ttgacgaaga tctgcacgac cactcgccac accgagaact gcgggtcgtc
     3661 gagcgtgccc tcgatcggga gctcgaggtt gatcatgccg ttgcggtcct tgagcagcgc
     3721 cactgcgagc aataccggca gcttggtcgc ggtcgggcta tccacgtgct cgccgaagat
     3781 cagctggtcg agcatgagct tgttgttcgc ggaaagcttg cgattgtcca gcttgtaatg
     3841 gacctccatc gacaacttcc ccttctggat cccgtagccc gcgtacttca cggaataggg
     3901 cgtcaacgac ggcaggtcga cgccggtggc cttggcggtg aggtccagcg aaagctcctt
     3961 tgcgaacgga ttgaccgtgc cacggatgtc gacgggtgca ctcctgtcga tcttcgcggt
     4021 cagctcgacg gttccggact gtgctgccgc gagcgcggag acggaaccgg ttacgtccgt
     4081 gaggtgcgcc gaataattcg gcttcacgaa gaaatccgaa tactccacct cgccattcga
     4141 cagccttatc cgcccgatgg aggcggggat ctcgcggcgc tcgcctgccg gggcaggtgg
     4201 cggcgcatcc gctgccgcaa tgacgcttcc ttgctgcgca ggcgttgccg acgccttctc
     4261 cgacgcatcg ggtgcgagca gacgctgcac gttgagcgtc gcgtcggcat tgaggatgag
     4321 gcgcgcgtag aacccgtcga gcgccacgtc gttcaacgcc aggttgaagg gagcggatgt
     4381 cacgtcgatg ccggtcaggc cgagcgactt ccagcgcacc agctcctccg agccggggcg
     4441 atcgagggaa tcgaagtcgt tcacgatgac gtcaccgacg aatcgaccct gcgggccgga
     4501 ggttccggcc tcgccgtagg tgatgcgccc cttcgcgtcc acggctccac tcgtgaggat
     4561 gacgttcgtc cgggactcga aataggatcg caggggcacg agatcgaggg ccgtcgcgtc
     4621 gatacggcaa tcggcgctcg ccggccgggc cgagaccacg ccattgagcc gcacgcgccc
     4681 cttcgcgccg actcgcgccg tgacatcgat tttcgccttg gaaccacgag cggtgtccag
     4741 gttttccgcg acgaccttcg cgtcggcgac gcgcagcttg accgccgggt ccggtacccg
     4801 atcctccaag tccacgccga atcgctcgag gagaagctgg cgcacgacga cgtgccagtc
     4861 cgtatcgccc gctttctcct tcgctgtctc cgcggcgtcc ggcggattcg cccggcccgg
     4921 ggctgtgcgc atcgcccgct cgaagtgcat cctgccgtcc gcatcgcgca gcaaccggat
     4981 ggcggccttc tgtgcctcga tcctttcgat ggttgccgtg cgcgcggcga ggtcgagggc
     5041 gatcccgtcg gcgctcgcgc gcgccatgcg ccagagcggt tcgcgttcgc tgcgtagcgc
     5101 aacgtcgaga tcggcgagca ccgcggagcc ttgcgcgagc gtgaatcgcg tcgccggact
     5161 ggtgccggcg tcgaaatcgg cgccgaagtc gagggcaccg cgcttgacgt ccatggcgag
     5221 cacgctcgtg gagtaagggc gcagcctggc cagcgcaagc ttcgtcaggt cgaagtgacc
     5281 gcgcgccgca ttgcccgcga agtccaccac gccgtgcgcg gcgaaatggg cgtcctcgga
     5341 atcgaaggcc acgtcgagag tgccgggagc gccctgggat gccagtttcg cgccggtgag
     5401 cgtgatagcc gacagtgtcg tcttgaacgg gggcgccacg gcctcgtcgg cgatgctcag
     5461 gtccccgccg gaaacgcggg tttcggtcac cgaccaggcc caggtcggac gcgcggcgtc
     5521 attcggcgcc ggcaccgccc tggtggcgga ggttccggga gtgtccgccg ctgcgctctc
     5581 cggtgtcact gcgagcagtt tctggatctc gagcgttcca tccgcaaggc gccgcaggtc
     5641 cagcatcggc ccgtcgacag cgatgcgatc gactgcgatg gagcggcgca ggaagtccag
     5701 gtttccgatt gccaccgcga tcgacctggc ggcaatgagc ggcgtggcct ctcttctagc
     5761 gatcgccaac cggtcgacgc gcgcgttgcc ggacagcgtg atgccgcgcg gcgtcccctt
     5821 ggacgtcacg aaggcaagcg atagacgggt cgtgagtgcg ccgtccgtca ggttgagccc
     5881 gctcggcagc ggcgcgtagg ccaggtaacg cggcagcggc agcgcgtcga gattgagctc
     5941 gagagtcgcc cgctgcagat cctcgaaggg cgacgttgca gtgccggtga gaccgaacgg
     6001 ggccccgtcg acggcaccgt gcagtcgcgg cgtgacccgg atctgcgcat cccgggaaag
     6061 gctcgacagg aacggcaggc cgatctcgag tttcgtcacg gcaatccggc gctgatgaac
     6121 cgcgtcgtcc agcaggatcg agccgtcctc gatctcgata ttgttcaccg cgaacctcga
     6181 ctccggctcg cgcgacggcg ccagcgcgcg ctcgatcagg tcctggatgc cgaaccggcc
     6241 atcggcgctg cgagcgatct cgacccgggg ctgcaccagc cggacggcat ccagaaccgg
     6301 cgcgcgcctc cagaggctct cagccgatac gtcgacgacc agggcctcga acttgagcag
     6361 cgtccggccg ggatcgcggt ccgtcagggt gaagtccgaa agccgcgcac gcagcgtgaa
     6421 ggggttgaat tcgaccttgc ctatcgtcgc ctggcgcccg agctcggtac gcgcaagcaa
     6481 ctcgatcttc gacctggcaa ccgacgggac cacgaagaag cccaccgccg cgtacagcgc
     6541 gacaagcccg gcgacggcga cgacgagcgt agccggccgc ctccaggcag tgggaatcac
     6601 gccccggtcg atccccggtt cgccgcgaca cccggcaggc gattggcgat tcgcgtgaaa
     6661 agcgcggcga atgcgcgttc ggaaaagccg cggtgcttca ggggctcggc attggcctcg
     6721 aaggaatcgg caatctgctt ccattcctcg ggaccgaggc acttgcttgc cgcgggcagg
     6781 accgtgttct cctcgaggcc catgtgctcc cagacggtgt ccgtgagggt gctcacggcg
     6841 ctcgccaccc cctcgaacgc gtccggctcg ttactctcga agcggtccag cgccgcgccg
     6901 agcgcctcca cgagggaacg ttccctgccg tgctggcgaa cgagttcctc cagggtgtcg
     6961 ttcagctcgg gagcgcgaag cccgagcagg cggaagaggt gggtctcctc cttcgggtgg
     7021 tgcagcgcct cgggaaaggc gaacatgtat tcgacgatca ggcgcaacag cgaaacgtcg
     7081 agtcgattgc cctgggcgcg gccctggctc accaggtgct ccaggccgcg aagcgccgtt
     7141 ccgatcgagc gatgctcgcc gtgtatcacc gccagggccc tgttctcgtc ggcgtcgcgc
     7201 tggttggcct gcacggtcgc gacgtggacc ggcaggtcgg agatctgcag gagcttctgc
     7261 gtgttgttcg tcgtgaacca gccgcgtacg ccgcgttggg gtccatggga actcacgaag
     7321 atcaggtcgc agccctccct ccgcgccgcc tccacgatcg ccagcggggg gtccccgctc
     7381 gtggcggcga cggcgccgca cttcattccc agggccgcgg ctgccgtgag tgccttggag
     7441 agtatcgcgt tggtctggcc gagctcggcc tcggaatggg tcatgttgcc catggagtgg
     7501 ttcggcgcgc cattgcctgc gccggcgtac tccggcgtgg cgtagaagaa ggtgattcgt
     7561 gcgcacagcg cccgggcgaa tgcgatcgaa gtcgtgaccg tctgggtggc gagatccgtg
     7621 tcgtccagcg caacgagaat gtgacgatac atggtggact gtcatccccc gggtaggtca
     7681 tgcattatgc attctccccc ggtaaccgca tcaagtgcgg gcgatgagcc ccccgccgcc
     7741 cgggtcgggc gccagatgct ccaggtaagc gtccctggcg ggtcgcaggt gcctgcggca
     7801 caacgccacg aggtgcacgc ggtccttcgc ggccagcgcg cggatcatgg cctggtgctc
     7861 ctcgcgagcc tgctcgcgaa aatcgggcga gacgagggcg ctgaagcgga tcgcgtgggt
     7921 ctggcgggca tattcggtga ttgcgttttc cagcacctcg ttgccggcca gggcaaagag
     7981 ttcgcggtgg aacgccaggt tggtgcgatg gacttcgcgc aggttgcggc gcgccaccgc
     8041 cttgtcgtgg tcgcgctgga tcgcggccag gcggtccagc gctgccgccg gcggaggcag
     8101 cggcatgcgc cgcgcggcct cggtctcgag gagctcgcgc atcgcataca actcccgcac
     8161 ttcccgaggc gtgaaggagc gcaccagcgc gccgatgttc ctgcgccgct cgaccaggcc
     8221 catgcgctcc agcgtcgcaa gcacctcgcg gaccacgtgg cgcttgagcc cgaagcgttc
     8281 gagcagctcg tcctcggtga tgcgttggcg cgggtgcagg cggccgagga cgatgtcctc
     8341 ctcgagcgcg tccgcggcgc cctgggcgct cgtggcgggc aggagtttcg ctgccgtcat
     8401 ggcccgggaa atgtcacttg cgcggggcgc gcaggaagtc gaagtccacg ccttcgtcgg
     8461 cctgcgtgac gcaatccagg aacagcttga ggtagccgcg ctccgcgctt ggaggcgtca
     8521 cgggattcgc ggcgcgccgc gcgcaaagct ccgcatcggt gacgagcagc gcgaccgaac
     8581 gcgcgggcac gtcgaggcgg acgcgatcgc cattgcgcac gtaggcgagc ggtccgccga
     8641 gcgctgactc cggcgtcacg tgcagcacga tcgttccggc cgcggtgccg ctcatgcgcc
     8701 cgtcggagat gcgcaccatg tccttcacgc cggcgcgcgc gagcttgcgc gggatgggca
     8761 ggtagccggc ctcgggcatc cccggcgcgc ccttgggccc gatgttcttg agcacgagga
     8821 tgtccccggc ggtcacgtcg aggtcgtccg aatcgatgcg cctcgccatg tcctcggcat
     8881 tctcgaagac caccgcgcgg ccctcgtgct gcatgagctt cgggtcggcg gccgactgct
     8941 tgatgatcgc cccgccgggc gcgaggttgc cgcgcagtac ggcgatgccg ccctgcgcga
     9001 agatcgggtt ggcgaggggc cggatcacgt cctgcctgaa gcccgggccc gaagcctcga
     9061 tctcctcgcc gatcgtgcgt ccggtgacgg tgagcgcgtc caggttcagc agggacttca
     9121 gttcgcgcag cagcgccggc acgccaccgg cgtgatggaa atcctccatg tagtggctgc
     9181 cggaaggctt gaggtcgacc agcaccggcg tctcgcgccc gatggcgtcg aggcgcgcga
     9241 gatccaccgg gatgcccagg cggccggcga tcgcggtgat gtggacgatg ccgttggtcg
     9301 atccgccgat cgcgagcagc acgcgcaagg cgttctccac ggatttcgcg gtgacgatgc
     9361 gcccgggcgt gaggcgcgcc ttcgcgagag ccacggccgt ggaaccggag cgctcggcga
     9421 tgcggatgcg atcggcggtc accgccgggg gcgaggcgct gccgggcagg gcgatgccca
     9481 gcgcctccgc gacgcaggcc atcgtgctgg cggtccccat caccgagcac gtgcctacgc
     9541 tcgccacgag ctggttgttc acgcgcgaga tctcggccgc gtcgatttcc tcggcgcggt
     9601 agcggcccca gtagcggcgg cagtccgtgc aggcgcccac gcgctcaccg tcgtgcgagc
     9661 cggtgagcat cgatccggtc accagctgga tggccggaac gcccgccgag caggcgccca
     9721 tcagctgcgc gggcacggtc ttgtcgcagc cgccgacgag gaccacggcg tccatgggct
     9781 gggcgcggat catctcctcg gtgtccatcg acatcaggtt gcgcaggaac atgctcgtgg
     9841 ggtacgcgaa gctctcgtgg atggagatgg tcgggaaggc cacgggcagg ccgccggcga
     9901 gcatcacgcc gcggttcacc gcctcgagca attgcgccgc gttgccgtgg caggggttga
     9961 agccgctcgc ggtgttggcg atgccgatga ccgggcgcga gagcgcctcg tccgtgaatc
    10021 ccgcgccctt gatgaaggcc ttgcgcagga agagggagaa gtccgtgtcc ccgtagctgg
    10081 tgaggccctt cgagaggccg gcggcgggcg gcttcgccgc cttcgaggag gggtcggtca
    10141 tggtcgctta ttatcgataa taatattgac aaacagtata cgcgctgcgc atagtcgggt
    10201 ccatgcaccc gcgcagcggc ggggcgcatc cgccacggca acaaccccgg agatcccgat
    10261 gacatcgcca ttcctgcgcc tcttcgcgaa gcgcgccgct tccctgattg ccgtcaccct
    10321 gctgttcgcg gcgggtggcg cgatggccca gtacccgaac aagtccatca agctgatcgt
    10381 gccgtacccg ccgggtggcg ccacggacgt catcggacgc gttctcgcgc agcgcctttc
    10441 gacggccctg ggccagcagg tgatcatcga caaccggggg ggcgccggcg gcaacatcgg
    10501 cgcggaggcc gtcgccaagt ccgcgcccga cggctacacc ctgctcatgg gggcccttac
    10561 ctcgcactcg atcatggcga ccctcgagaa gggaacgctg cgttacgacc tggccaggga
    10621 cctcgtgccc gtggcggtgg tgggttcggt tccgctcgtg tgggtggtct acccgccgct
    10681 tcccgtgaac agcctgaagg aacttgtcgc ctatgcgaag gccaatcccg gcaagctcac
    10741 ctacgcgtcc tcgggcgccg gcgcgccgca gcgcatgtgc gcggaactct tccggctcaa
    10801 gatcggtgcg gacatgatcc acgtcccgta caagggaagc ggcccggcga tgggcgacct
    10861 cgtgggcggc caggtggtga cgatgtgcga gaccgtgccg gcctcgcttc agctcatcaa
    10921 ggcgggcaag ctgcgcgccc ttgcggtgac cactgcgcag cgcatctcgc agctccccga
    10981 cgtgcctacg gctgccgagg cgggcacgcc cggcatcgaa gtgagctcgc tcttcggcgt
    11041 gatggcgccc gcgggaacgc cggcaccgat cattgcccgg ctcaatgcgg aactgcagaa
    11101 gatcctggcc accccggagg cgcaggagca gctcctgcaa cagggcgcct acgcggtgca
    11161 gatgagcccc gagcaatccg ccgctcgcat caggcaggaa atcgacctgt gggcaagggt
    11221 catccaggag gcgaagatca agagcgactg agacccgctc gcgccgggac ggcgccccgg
    11281 gaggcgccgc ccccgtcact cgaactgtga gcgttcgagg cgaaggtagg cgtggctggc
    11341 gttctgcgga tgcagcgcct cgtactggtc ccagtgcgcg aattcgggaa ggtcgcagcg
    11401 gtggcgcagt tcggccaggc tcaccccgtc cctcaacagc gtctcgacgc gcccttcgag
    11461 tgccgcgaag tatcggtcga aggcggggat gtcggcgcac gtgcccgggg gcccgtagcc
    11521 gggaacaagg tgccggcatc gcatcgacgc gaattgcgcg agggtcctgc gccacgccgc
    11581 cgggttcgcg tcgcgcatat cgggaacgcg ttcgatcgcg accaggttgc cggcgatcag
    11641 ggtcgaagtc gtttcgtcga aaaccgcgat cgcgccggga acgctcgacc agcgtggtgc
    11701 gatcagccgc agccggcgcc cgatcaggtc gagcgtttcg tcgccgtcga tcaatcgatc
    11761 cggcgtgacg acccgcgtcg ccgccatggc ctcctcgccg agcagcgggc gcagcctgcg
    11821 caggcaggtc tcgcagcggg ccgccatcaa cttcgcgccg ctgcggtggg cgaggacggg
    11881 aatgccgcgt tcctggaatg cggcggcgcc gaagatggac tcctggccgg ggtgcgagag
    11941 gatcgcgaga cggatgggcc ggttcgagat gctttccact gccgcgatga tgtcgccgcc
    12001 ctggcggtgg gagatcccgg tatcgaccac gatgacgccg cgcggaccca cgacgaaggc
    12061 gacattcgcg gtgcgtccgc catttttccg ggtgatctcg ccgccgcttc ccggcaggac
    12121 gtagatgccc ggcgccacca tgaccgtggc cggctgcacg gaacgtgtgc cgggagtggc
    12181 gcaggacacg acgagaatgc cgcagagcgc cgcaatgaat gccgcctgca tcgcggcgag
    12241 cagtcttcgg cggcatgccg cgacgcgcgg cgacgatggg cgtgcgatgt tgttgcagtg
    12301 cattgtgtcg aagtcccccc gtcaacggcg cacatctgca cctgaaggtc acggcctttc
    12361 gctgatcctg ttgcgcggaa atgcggcagg gcaacatgac attccggtcg atgccgcgca
    12421 acgcgctggc aactttctgc aaacaaattg actttgatca agtgtaggac gaaactttcg
    12481 ccgcaagcga tcggagccgg gaaacccagg ggagtatggc cgccgatcgc gttgtacttt
    12541 cttttgacct gaccaccgga ggatgaccag acaatcggag gaaacaatga ctagcgcctc
    12601 aacgcggtta gcagttgcaa tctcaggttt tgcactggcg gcaccaggca tcgcggcggc
    12661 gaacgcggat ctcgaaaagc agattgccga ccccaacaac tgggcgatgc aggccggcga
    12721 catgtacaac cagcggtaca gcaagctgac acagatcaac gcacagaacg ttggcaagat
    12781 gcaggtggcg tggatgtttt ccaccggcgt gctgcgtggc cacgagggtt cccccctggt
    12841 cgcgaacggc atgatgtacg tgcatacgcc ttttcccaac aaggtgttcg ccatcgacct
    12901 cgacacgcag aagatcaagt ggcgctacga gccgaagcag gatgtcgcgg tgatcccgca
    12961 gatgtgctgc gataccgtga accgcggcgt cgcatacgcg gagaagaaga tcttcctgca
    13021 acaggccgac agcacgctgg tcgcgctcga cgcgaacaac gggaaggtga tctggtcggt
    13081 caagaacggg gatccgaagg ccggcgcggt caataccaac gcgccgcacg tcttcaagga
    13141 caaggtgatc accgggattt ccggcggcga atggggtgtg cgcggcttcc tcgccgccta
    13201 tgacatcaat acgggcaagc aggtgtggaa ggcatacagc accgggcccg ataacgagat
    13261 gctgatcgat cccgacaaga cgatgacctg gaccgacggc gcgctcaagc cggtcggcaa
    13321 ggactcttcg ctgaagacgt ggaagggcga ccagtggaag acgggcggcg gcacgacgtg
    13381 gggctggtac agctacgaca aggcgctcaa cctggtctac tacggaacgg gcaacccgtc
    13441 gacctggaac ccggcgcagc gccccggcga caacaagtgg tcgatgaccg tgttcgcacg
    13501 cgatgtcgac acgggcgtgg caaagtgggc ttaccagatg acgccgtacg acgagtggga
    13561 tttcgacggc atcaatgaaa tgatcctggc cgacatcaac gtcaagggca aggccaccaa
    13621 ggcactcgtc cacttcgacc gcaacggctt cgcctacacg ctggatcgcg ccacgggcgc
    13681 cctgctcgtc gccgagaaat acgatccgac ggtgaactgg gcgacgcacg tcgacatgaa
    13741 gaccggccgg ccgcaggtcg tgaagcagta cagcacgcag gcgcagggcc agga
//
LOCUS       DASUEV010000023         8583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1037704, whole genome shotgun sequence.
ACCESSION   DASUEV010000023 DASUEV010000000
VERSION     DASUEV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037704"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFV55_00620"
     CDS             complement(<1..377)
                     /locus_tag="VFV55_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX4942821.1"
                     /translation="MPIRTCVSAVATVLAAFASLSLHAQTTIPSSRLGKIHLLPATLE
                     TTQWGWFDNAQAPVLTINSGDTVVMETMMHSHNQVVPGRTIEEIKKLRTDHPGRGPHT
                     LTGPIFVNGAEPGDVLKVTLNRIV"
     gene            complement(860..2452)
                     /locus_tag="VFV55_00625"
     CDS             complement(860..2452)
                     /locus_tag="VFV55_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4942822.1"
                     /translation="MRRTLATAALIAALSAAPLAGAKTLRWSSQGDYVTADPMAQNEL
                     LTNSINGHVYEPLVTRGKQLEILPALATSWKQTSPTVWVFSLRKGVKWHDGSDFTADD
                     VVFSMKRLQGETSNFRVYGNAVGAARKIDDYTVEFTTPVPNPVMLEMLANSLFMMSKS
                     WCEKHKVEKSQDFKAKEETYTVRNAMGTGPYILVSREPDVKSVFKQNTNWWGLKEKGY
                     FDGNVTDIIYSPVKSEGTRMAALLSGDLDFILDPPVQDVEKLKKDKNVKVYEGRENRI
                     VFLGMDQARDQLLYSDVKGKNPFKDRRVREAMYRLIDVNAINKAVMRGLSVPTAINLP
                     NPTRAGIPASMDKRYPYDVAAAKKLLAEAGYPNGFEVTIDCPNNRYINDEKICVAVAG
                     MVSKGGIRMKVNAMPRAQYFPKVQNLDTSMYMLGWGGATTDAIFTLQPVLHSRNDKGD
                     GDYNWGNYKYPEFDAMIDEAKGETDTKKRQETIIKAMKFHHDNVLHIPLHLQVIPWAS
                     RANVEVIHRADNWLQATWVKIK"
     gene            2582..4069
                     /locus_tag="VFV55_00630"
     CDS             2582..4069
                     /locus_tag="VFV55_00630"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX4942823.1"
                     /translation="MNAVVDRKVSPPALKDPGLFRDRCYIDGAWAQADSGRRFDVDNP
                     GDGSVVGSVPDMGAAETRRAIEAAERALPAWRALPAKERSKIVRKWYDLIVANADDLA
                     LILTTEQGKPLAEAKGEIVYGASFVEWFAEEAKRIYGDTIPSPTNDRRLIVLKQPIGV
                     CAAITPWNFPNAMITRKMAPGLAVGCTFVLKPAEQTPFSALALAELAERAGFPKGVIN
                     IVTGDAPAIGKELCANPVVKKVTFTGSTEVGRILMRQSADTVKKMSLELGGNAPFIVF
                     DDADLDAAADGAMASKYRNAGQTCVCANRIYVQDGVYDAFAAKLGERMKSLKVGRGTE
                     TGVNLGPLIDAQGLAKVEEHVADAVAKGAKVVVGGKRSALGGRFYEPTLLTGVTAEMK
                     VAREETFGPVAPLFRFKDEAEALKLANATEFGLAAYFYSRDLGRAFRVAEGIESGMVG
                     VNTGMISNEVAPFGGVKQSGLGREGSRYGVEDFLEIKYVCLAGIQ"
     gene            4157..4606
                     /locus_tag="VFV55_00635"
     CDS             4157..4606
                     /locus_tag="VFV55_00635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX4942824.1"
                     /translation="MKKLAIAAAVALLAANACAQTATAPAAATAPVEVKELIKKDTKL
                     GEGKVAEKGKAVLVHYTGWLYDPKAPGQKGAQFDTSVGKATPFGFVIGAGRVIKGWDE
                     GVPGMKEGGKRTLIIPPAMAYGEKGAGGLIPPNATLLFEVEFIKAVN"
     gene            4626..5255
                     /locus_tag="VFV55_00640"
     CDS             4626..5255
                     /locus_tag="VFV55_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX4942825.1"
                     /translation="MLVTSTGFRNDGAIPAEYAFCAMDAKTHATLSQNRNPQLSWTGA
                     PAGTKSFAIVCHDRDVPSRGDDVNQEGRTVPASLARVDFFHWVLVDLPVSVTSVAAGE
                     FASSVTARGKPGPAAAHGARQGINDYTGWFAADKDMAGNYFGYDGPCPPWNDEIPHHY
                     VFTVFALDVAKLAVDGAFTGAQVREAMKGHVLAQAAITGRYTLNPAVRL"
     gene            5255..6025
                     /locus_tag="VFV55_00645"
     CDS             5255..6025
                     /locus_tag="VFV55_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX4942826.1"
                     /translation="MPVGILVIGDEILSGKRQDKHFAKAIEILGKRGIELDWAEYIGD
                     DPERITATLRRSFATQDVVFSFGGIGATPDDHTRQCAAAALGRPLARHPDAVKEIEAR
                     FGEAAYPKRVLMAEFPEGADIVPNPVNRIAGFSVGTHYFLPGFPEMAWAMMEWALDTK
                     HHALFRDHLPFEEAIIVSGAGESDLLEIMNAVVRDYPELKLSSLPTFSGGGARLIELA
                     VRGERARVPVAMTFVRDAIAKLGYSVTDRPAKPSPVIP"
     gene            6042..6641
                     /locus_tag="VFV55_00650"
     CDS             6042..6641
                     /locus_tag="VFV55_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4942827.1"
                     /translation="MEQLCNVLRSLTRRAFVIAVLAFIATPAVADEDAAWAALRAGGH
                     VALMRHASTEAGLGDPAGYNLDDCATQRNLSAQGREEARRIGERFRREGVVVERAYSS
                     PWCRCRDTAYEAFRKSEDWTALASFFDEPHREPELTKRVKMRIGGYSTQKPKGTIVMV
                     THNVNIAALTGLSVGTGEIVVLRPDGCCGLKVVGRIAIR"
     gene            6660..7265
                     /locus_tag="VFV55_00655"
     CDS             6660..7265
                     /locus_tag="VFV55_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4942828.1"
                     /translation="MGMQSKDLSRTATVPERESALRLLLTRYSVSAKHLGEPGPSPDE
                     IWTMAMAALRAPDHKKRIPFRFVIARDVGLERLADLFEDYGRRKGKSGDDLAAERARA
                     MQAPVAIAVIARIGSDDPEVPPFEQWACVGGAIGNALNALHFMGYSGKMVSGARAADP
                     AIVAAYCGSGETLVGWIAAGTAKGPAKPRGEVDPCGIIAEF"
     gene            complement(7282..7677)
                     /locus_tag="VFV55_00660"
     CDS             complement(7282..7677)
                     /locus_tag="VFV55_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX4942829.1"
                     /translation="MSAGAGSLRLDKFLWAARFFKTRGLAAEAIDAGRVSVNGDRAKP
                     ARNVKPGDALAIRRPPFEHLVMVLAVSDRRGPAAEAQKLYEETAESRAKRQAVAAELK
                     SMPPPVFKGRPTKKDRRTLERFVRRQDVD"
     gene            complement(7677..8243)
                     /locus_tag="VFV55_00665"
     CDS             complement(7677..8243)
                     /locus_tag="VFV55_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4942830.1"
                     /translation="MTTIVVVSKGGKAAIAADSLTTFGSTRLGPAYDRNPHKIITYAD
                     SFIGVAGSAAHQLVLENLLGKNPDLALHGKDAIYESFRRLHPMLKDEAFLNPKEEEDD
                     PYESSQMTVMIANPSGIFAVYSMREVFEFDRFWAIGSGRDFALGAMYVSYPRAKSADL
                     VARIGVEAGAEYDTGTSLPMALHTVTLV"
     gene            complement(8240..>8583)
                     /locus_tag="VFV55_00670"
     CDS             complement(8240..>8583)
                     /locus_tag="VFV55_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4942831.1"
                     /translation="AGGVIQYTAAGIVTFVAACATETRVVQWTPQFVFALAWLVLVLS
                     TGAIGILFAMIRHGEASRVSSLFFLTPPVTAIMAFLVFGERLPPIALAGLAVSAAGVA
                     LVTRGARMGKP"
BASE COUNT         1400 a         2852 c         2896 g         1435 t
ORIGIN      
        1 acgatgcggt tcagcgtcac cttcagcacg tcgcccggct cggcgccgtt cacgaagatg
       61 gggcccgtca gcgtgtgcgg cccgcgcccg gggtggtcgg tgcgcagctt cttgatctcc
      121 tcgatggtgc ggcccggcac cacctggttg tgcgagtgca tcatcgtttc catgaccacg
      181 gtgtcgccgg aattgatggt gagcaccggc gcctgcgcgt tgtcgaacca gccccactgc
      241 gtcgtctcga gcgtcgcggg cagcaggtgg atcttgccca gccggctcga cggaatcgtc
      301 gtctgggcgt gtagcgaaag cgaagcgaat gcagcgagga ctgtggcgac ggcggacacg
      361 caggtacgga ttggcacggc tctctcccca ggtagattga cagcgcggcc aacatactcc
      421 attcgcccgc ggcaaaaaag gacacgcgtc aagcgctaag cggcaccggg aggcggtggc
      481 ggcggaagtc gatcgcaatc cggggcaggt gaggtgaggc ggcggcggtg gaagtcgatc
      541 gcaatcctgg gcacgcaagc gtgaccgtcg gatgggtgcc cggaaagtcg aggccagcca
      601 aacacgtctg tcctcgactt tccacccatc tagagacgag gtcgctgcgc tgcctcgtcc
      661 gcgacggtcg cgtgccaagg attgcgatcg acttccaccg ccaccgcctc cacgctccgt
      721 ggatacagca gcgagtgaga caacgccgga ccgcttctcg tgcgctgccg gatgcgccac
      781 gctgcccatc cccggccctc gtcctaattc gccgcgtaca tctggcacgc accgcggtcg
      841 ggtcccccgc ttccgctcgc tacttgatct tcacccaggt ggcctgcagc cagttgtcgg
      901 cgcggtggat cacctcgacg ttggcgcgcg aggcccaggg gatcacctgc aggtgcagcg
      961 ggatgtgcag cacgttgtcg tggtggaatt tcatcgcctt gatgatcgtc tcctggcgct
     1021 tcttcgtgtc cgtctcgccc ttcgcctcgt cgatcatcgc gtcgaactcg gggtacttgt
     1081 agttgcccca gttgtagtcg ccgtcgccct tgtcgttgcg gctgtgcagc accggctgca
     1141 gggtgaagat cgcgtccgtc gtggcgcctc cccagccgag catgtacatg ctggtatcga
     1201 ggttctgcac cttcgggaag tactgggcgc gcggcatcgc gttcaccttc atccggatgc
     1261 cgcccttgga caccatgccg gccacggcca cgcagatctt ctcgtcgttg atgtagcggt
     1321 tgttcgggca gtcgatggtc acctcgaagc cgttcggata gccggcctcg gcgagcagtt
     1381 tcttcgccgc cgccacgtcg tacgggtagc gcttgtccat cgacgcgggg atgccggcgc
     1441 gggtgggatt gggcaggttt atcgcggtgg gcacggagag cccgcgcatc accgccttgt
     1501 tgatcgcgtt cacgtcgatc aggcggtaca tcgcttcgcg cacgcgccgg tccttgaacg
     1561 ggttcttgcc cttcacgtcg gagtagagca gctggtcgcg cgcctgatcc atgccgagga
     1621 acacgatgcg gttctcgcgg ccctcgtaca ccttcacgtt cttgtccttc ttgagcttct
     1681 cgacgtcctg cacgggcggg tcgaggatga agtcgaggtc cccggagagg agggccgcca
     1741 tgcgcgtgcc ttccgacttg accggcgagt agatgatgtc ggtgacgttg ccgtcgaagt
     1801 aacccttttc cttcaggccc caccaattgg tgttctgctt gaagacgctc ttcacgtccg
     1861 gctcgcgcga taccaggatg taaggacccg tgcccatggc gttacgcacg gtgtaggttt
     1921 cctccttggc cttgaagtcc tggctcttct cgaccttgtg cttctcgcac cagctcttgc
     1981 tcatcatgaa caggctgttg gcgagcatct cgagcatcac cgggttgggc accggggtgg
     2041 tgaactccac cgtgtagtcg tcgatcttcc tcgccgcgcc caccgcgttg ccgtagacgc
     2101 ggaaattgga ggtctcgccc tgcaggcgct tcatggagaa gacgacgtcg tcggcggtga
     2161 agtccgaccc gtcatgccac ttcacgccct tcctcaggct gaagacccag acggtcgggc
     2221 tggtctgctt ccagctcgtg gcgagcgccg ggaggatctc gagttgcttg ccgcgcgtga
     2281 ccagcggctc gtacacgtgg ccgttgatcg agttggtgag cagctcgttc tgggccatgg
     2341 gatcggcggt gacgtagtcg ccctggctgg accagcgcag ggttttcgcg ccggcgagcg
     2401 gggcggcgga cagggcggca atgagggcgg cggtggccag ggtgcgtcgc atcgtggttc
     2461 tcctgatgga agagcggccc atgatactcg ctggccgcga tggtccggca agggcccgca
     2521 ggggctcgga gggcgtgccc gccgcgctat actttctccc ttccccgaag gaggaaatgc
     2581 catgaacgcc gtcgtcgatc gcaaggtttc tccgcccgcc ctcaaggacc ccggcctgtt
     2641 ccgtgaccgt tgctacatcg acggcgcatg ggcgcaggcg gattcgggcc gccgcttcga
     2701 cgtggacaac cctggcgacg ggtccgtggt cggttccgta ccggacatgg gcgcggccga
     2761 gacgaggcgc gccatcgagg cggccgaacg ggcgctgccc gcctggcgcg cgctgcccgc
     2821 gaaggagcgc tcgaagatcg tgcgcaagtg gtacgacctc atcgtggcga acgccgacga
     2881 cctcgcgctc atcctcacca cggagcaggg caagccgctg gccgaggcca agggcgagat
     2941 cgtgtacggc gcctcgttcg tcgagtggtt cgcggaggag gccaagcgca tctacggcga
     3001 caccattccc agccccacca atgaccggcg cctcatcgtg ctgaagcagc ccatcggcgt
     3061 gtgcgccgcg atcacgccgt ggaatttccc gaatgcgatg atcacccgca agatggcgcc
     3121 ggggctcgcg gtgggctgca ccttcgtgct caagcccgcg gaacagacgc cgttctcggc
     3181 gctggccctg gcggagcttg ccgagcgcgc cggcttcccc aagggcgtca tcaacatcgt
     3241 caccggtgat gcgcccgcga tcggcaagga gctgtgcgcc aacccggtgg tgaagaaggt
     3301 caccttcacc ggttccacgg aagtgggccg catcctcatg cgccagagcg cggacacggt
     3361 gaagaagatg tcgctggagc tgggcggcaa cgcgccgttc atcgtcttcg acgacgccga
     3421 cctcgatgcg gccgccgacg gtgcgatggc ctcgaagtac cgcaacgccg ggcagacctg
     3481 cgtgtgcgcc aaccgcatct acgtacagga cggggtgtac gacgcgttcg ccgcgaagct
     3541 cggcgagcgc atgaagtcgc tcaaggtcgg caggggaacg gaaaccggcg tgaacctggg
     3601 accgctcatc gacgcgcagg ggctcgccaa ggtcgaggag cacgtggccg acgcggttgc
     3661 caagggcgcg aaggtggtgg tgggcggcaa gcgttccgcc ctgggcgggc gcttctacga
     3721 gcccacgctc ctcaccggcg tgaccgccga gatgaaagtc gcgcgcgagg aaaccttcgg
     3781 cccggtggca ccgctcttcc gcttcaagga cgaggccgag gcgctcaagc tcgccaatgc
     3841 caccgagttc gggctcgccg cctacttcta ctcgcgcgac ctcgggcgcg ccttccgcgt
     3901 tgcggaagga atcgagtcgg gaatggtggg cgtcaacacg ggcatgatct ccaatgaagt
     3961 cgcgcccttc ggcggcgtga agcaatccgg gctagggcgc gaggggtccc gatacggcgt
     4021 cgaggacttc ctcgagatca agtatgtgtg cctcgcgggg atccaatagc cgcatcactc
     4081 accagcgtga cctcactcgc ctggccccat accattcctg aataccaacc acgaaaccgc
     4141 aaggaaagga attgaattga aaaaactcgc cattgccgcc gccgtcgccc tgctcgccgc
     4201 caatgcatgt gcgcagacgg ccacggcgcc tgccgccgcc accgctcccg tcgaagtgaa
     4261 ggagctgatc aagaaggaca cgaagctggg cgaaggcaag gtcgccgaga agggcaaggc
     4321 cgtcctggtg cactacaccg gctggctcta cgacccgaag gcgcccggcc agaagggcgc
     4381 ccagttcgac acctcggtgg gaaaggccac gcccttcggc ttcgtcatcg gggcgggccg
     4441 cgtcatcaag ggctgggacg agggcgttcc cggcatgaag gaaggtggca agcgcacgct
     4501 catcatcccg ccggccatgg cttacggcga gaagggagcc ggcggcctca ttccgcccaa
     4561 tgcaacgctg ctgttcgagg tggagttcat caaggcggtc aattgaccga ggggagggca
     4621 cgggcatgct ggtcaccagt acaggcttca ggaacgatgg cgccatcccc gcggaatacg
     4681 cgttctgcgc aatggacgcg aagacgcacg ccaccctctc gcagaaccgc aacccgcagc
     4741 tttcgtggac gggtgcaccc gcgggcacga agtccttcgc gatcgtctgc cacgaccgcg
     4801 acgttccgag caggggcgac gacgtgaacc aggaggggcg aacggtcccg gcctcgctgg
     4861 cgcgcgtcga tttcttccac tgggtgctgg tggacctgcc cgtatccgtg acctcggtcg
     4921 ctgccggcga attcgcgtcg agcgtgacgg cgcgcggcaa gcccggcccg gcggcggcgc
     4981 acggtgcgcg ccagggcatc aacgactaca ccggctggtt cgccgccgac aaggacatgg
     5041 ccggcaacta cttcggctac gacggcccgt gccccccgtg gaacgacgag atcccgcacc
     5101 actacgtctt caccgtcttc gcgctggacg tggcgaagct cgcggtggac ggggccttca
     5161 ccggtgccca ggttcgcgaa gccatgaagg ggcacgtcct cgcgcaggcc gcgatcaccg
     5221 ggcgttatac cctcaacccc gccgtcaggc tctagtgccc gtcggcatcc tggtcatcgg
     5281 cgacgagatc ctgtcgggaa agcggcagga caagcatttc gccaaggcga tcgagatcct
     5341 cggcaagcgc gggatcgagc tcgactgggc ggaatacatc ggcgacgacc cggagcgcat
     5401 caccgccacc ctgcggcgca gctttgccac gcaagacgtc gtgttttcct tcggcggcat
     5461 cggcgcgacc ccggacgacc acacgcgcca gtgcgcggcc gcggcgctgg gccgccccct
     5521 cgcgcgacat cccgatgcgg tgaaggaaat cgaggcgcgc ttcggcgagg ctgcctatcc
     5581 gaagcgcgtg ctcatggccg agttcccgga gggcgccgac atcgtcccca acccggtgaa
     5641 ccgcatcgcc ggcttcagcg tgggaacgca ctacttcctg cccggattcc cggaaatggc
     5701 gtgggcgatg atggaatggg cgctcgacac gaagcatcac gcgctcttcc gcgaccacct
     5761 gcctttcgaa gaggcgatca tcgtcagcgg ggcgggcgaa tccgacctgc tcgaaatcat
     5821 gaacgcggtc gtgcgggact atcccgagct caagctgtcg agcctcccga cgttctcggg
     5881 cggcggcgcc cgcctcatcg agctcgcggt gcgcggcgag cgcgcgcgcg tcccggtggc
     5941 gatgacgttc gttcgcgacg ccatcgcgaa gctcgggtat tcggtcacgg accggcccgc
     6001 gaagccctca cccgtcattc cctgacctgg aggggaaacc catggagcaa ctttgtaacg
     6061 tcctccggtc gctcacgcga cgcgcctttg tcattgcggt ccttgccttc atcgccacgc
     6121 ccgccgttgc cgacgaggat gcggcctggg ccgcgcttcg tgccggcggc cacgtggcgc
     6181 tgatgcgcca tgcctccacc gaggccggcc ttggcgaccc cgccggctac aacctcgacg
     6241 actgcgccac gcagcgcaat ctctccgccc aggggcggga ggaagcgcgg cgcatcggcg
     6301 agcgtttccg gcgcgagggc gtcgtcgtgg aaagggccta ctcgagcccg tggtgccgct
     6361 gccgggatac cgcgtacgaa gcgttccgca agtccgagga ctggacggcg ctcgcgagct
     6421 tcttcgacga gccgcaccgc gagcccgagc tcacgaaacg cgtgaagatg cgcatcggcg
     6481 gctactcgac gcaaaagccc aagggcacga tcgtgatggt cacccacaac gtgaacatcg
     6541 cggcgctcac cgggctttcc gtcggcacgg gcgagatcgt cgtcctgcgc cccgacgggt
     6601 gttgcggcct caaggtggtc gggcggatcg cgatccgttg agcgtgccag gaggatgcca
     6661 tgggcatgca gtcgaaggat ctctcccgga cggcaactgt tcccgagcgt gaatcggcat
     6721 tgcgcctgct cctgacccgt tactcggtga gcgcgaagca cctcggggaa cccgggccgt
     6781 ccccggacga gatctggacg atggcaatgg cggcgctgcg cgcgccggac cacaagaagc
     6841 gcattccctt ccgatttgtc attgcgcgcg atgtgggcct cgagcggctg gccgatctct
     6901 tcgaggatta cggccgtcgc aagggcaagt ccggcgacga cctggcggcg gaacgtgccc
     6961 gggcgatgca ggccccggtg gcgatcgccg tcatcgcccg catcggctcc gacgaccccg
     7021 aggtgccgcc gttcgagcag tgggcgtgcg tgggcggtgc gatcggcaac gcgctgaacg
     7081 ccctgcactt catgggctac tccggcaaga tggtttccgg cgcgcgtgcc gccgacccgg
     7141 cgatcgtcgc agcctactgc gggagcggcg agacgctggt gggttggatc gccgccggca
     7201 cggcgaaggg tcccgcgaag ccgcgcggcg aggtggaccc ctgcggcata atcgccgaat
     7261 tctgaattcc gcgaacccga gtcagtccac gtcctgccgc cggacgaagc gctcgagcgt
     7321 gcgccggtcc ttcttggtgg gccggccctt gaacaccggg ggcggcatcg acttgagctc
     7381 ggcggccacc gcctgccgct tcgcgcggct ttccgccgtt tcctcgtaga gcttctgcgc
     7441 ttccgcggcc gggccgcgcc ggtcggaaac ggccagcacc atcaccagat gctcgaaggg
     7501 cgggcgccgg atcgcgagcg cgtcgcccgg tttcacgttc ctcgcgggtt tcgcccgatc
     7561 gccgttcacg ctcacccggc cggcatcgat cgcctcggcc gccaggccgc gcgtcttgaa
     7621 gaagcgcgcc gcccagagga acttgtccag gcgaagggat ccggcgcctg cgctcatcag
     7681 acgagcgtca ccgtgtgcag ggccatcggc agcgacgtgc cggtgtcgta ttccgccccg
     7741 gcctccacgc cgatcctcgc caccagatcc gcgctcttcg cgcggggata ggacacgtac
     7801 atggcgccca gcgcgaaatc gcgccccgag ccgatggccc agaagcggtc gaactcgaac
     7861 acttcgcgca tcgaatagac ggcgaagatg cccgaggggt tggcgatcat gacggtcatc
     7921 tgcgaggact cgtagggatc gtcctcctcc tccttgggat tgaggaaggc ctcgtccttg
     7981 agcatcgggt gcaggcgccg gaaggattcg tagattgcgt ccttgccgtg cagcgcgagg
     8041 tcggggttct tgcccagcag gttttccagc accagctggt gcgcggcgct gcccgcgacg
     8101 ccgatgaacg aatcggcgta ggtgatgatc ttgtgcggat tgcggtcgta ggcgggcccc
     8161 agccgcgtgg atccgaaggt ggtgagggag tccgcggcga tggccgcctt tccccccttg
     8221 ctgacgacga cgattgtggt catggtttgc ccattctagc gccgcgcgtg acgagggcca
     8281 cccccgccgc cgaaaccgcc agcccggcca gggcgatcgg gggcaggcgt tcgccgaaaa
     8341 ccaggaaggc catgatggcc gtcacgggcg gggtgaggaa gaagaggctc gagacgcgcg
     8401 aggcctcgcc gtgccggatc atggcgaaaa ggatgccgat ggcgcccgtc gagagcacga
     8461 ggaccagcca cgcaagcgcg aacacgaact gcggtgtcca ctgcaccacc ctcgtctcgg
     8521 tcgcgcaggc ggcgacgaag gtgacgatgc ccgcggccgt gtactggatc acgcccccgg
     8581 cga
//
LOCUS       DASUEV010000024        16721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_923314, whole genome shotgun sequence.
ACCESSION   DASUEV010000024 DASUEV010000000
VERSION     DASUEV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 16721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16721
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923314"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..1789)
                     /locus_tag="VFV55_00675"
     CDS             complement(332..1789)
                     /locus_tag="VFV55_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4942832.1"
                     /translation="MRIRAVFDRINPLAGVAGMMVIGTLLVTTIFFTLFDLPWIAFLT
                     GMLSAGAIGLVSRLAYAETALAEVRESVRYIDDELPVMIVYVTTDGTVRFHNQAFRYW
                     LRARRDSINGRPLRSVVGLTTFGQMKAGIDSALAGRAKHETRIHEGLGSPESRLSTQY
                     LPHVGAKGEIVGAFVVQTDISSVEAPSVATPASVAAPPPDTVPGTASEIPPSSPPAPN
                     GAEHERRIFLNTMTEELTDWKNAGDRLRAALDGNEFSLYTQAIVPVAAGAQGEPYYEI
                     LLRLREEEEGLMPPGAFLPLAEEYGLLPDLDRWVVKNVLDWAGASPRRQAATYSLNVS
                     GPTIGNADFPDFVVAALHQRGLKGSLLCFEITESDAVARPRDTVRFISVIGEAGCRTA
                     LCGFGQNADSFALLKRLRVDVLKIDGDIILGLLRNSVDLIKLKAITRVARATNRLTIA
                     EFVENEETLAKLREYGVDFAQGFGISRPVPLSDLP"
     gene            1932..3038
                     /locus_tag="VFV55_00680"
     CDS             1932..3038
                     /locus_tag="VFV55_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942833.1"
                     /translation="MSPTTHEQASGSPVPGLEGWPVPNHFSIVSIAHGGETERARIEM
                     GDGRELTGDAVRLDSVATMLEFRPDGAPASQFIRFSSFKKLCLTRPVELERLRLAIPP
                     ENVDAYPSHAGLNFTIRFKDGSEFSSPILGLVARRIGLFPYLTGEPGSVLRWFMPAEA
                     TSGCRIGDALGKILVERAVVSSDIVEAGLERQRRQRATKFGEFLCRQGIVTREQVEAC
                     LLAQSGQRHLKLGEVLVARGYVTAEQRDQALADQAVERGMPLGEILVQMGAVARKVLR
                     QALADQFGLPWVSLRGFRFEREAIDAISPEAARRHLVVPLYRSGVRIVLALSNPVDWE
                     AVEEIESASGSRVDPVLSSREDLREAIARHYGPG"
     gene            complement(3054..3803)
                     /gene="phbB"
                     /locus_tag="VFV55_00685"
     CDS             complement(3054..3803)
                     /gene="phbB"
                     /locus_tag="VFV55_00685"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX4942834.1"
                     /translation="MDKKIALVTGGVGGIGSAICKRLAQQGNFVVANYAIPGSESKYL
                     ANMAAAGLNGGSTALAFGDVSDYDAMGEMVANIAREHGPVDILVNCAGITRDSTFRKM
                     TPEQWHLVIETNLTSVFNVTRHVIDGMCDRGWGRVINISSVNAVRGQFGQTNYSAAKA
                     GILGFTKALAQEVVRKGVTVNAISPGYVETEMVAAIREDVRKQIVAQIPAGRLAMPDE
                     VADSVAFLASDKASYITGTNLSVNGGLHMYA"
     gene            complement(3949..4470)
                     /gene="phaP"
                     /locus_tag="VFV55_00690"
     CDS             complement(3949..4470)
                     /gene="phaP"
                     /locus_tag="VFV55_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEX4942835.1"
                     /translation="MVNLAEQMNELNNTAVLSAARLSKLSLDSAERLLALQLAFAKTS
                     LGDATRTARAAAGSNDVQQLLALRTKAAETAMSQWLEYSRGLYQVASDAQSELSKLAE
                     ERMAELQRNVTDTVEQAAKSAPAGSDVAVAAMKSSLAAATAAFDSFSKAARHAASFTD
                     AGVKAATAPKGRK"
     gene            4712..5332
                     /gene="phaR"
                     /locus_tag="VFV55_00695"
     CDS             4712..5332
                     /gene="phaR"
                     /locus_tag="VFV55_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX4942836.1"
                     /translation="MASPRIIKKYPNRRLYDTETSTYITLAEVKDLVLQYMDFQVQDA
                     KSGEDLTRAILLQIILEEESGGGPMFSTDMLSNIIRYYGHSMQGLMGSYLERSIHAFH
                     EAQKRFQAQAQVLIPKVPADAFANMMAGGQPIPQVMGGYLEKGAKAVIDMQEELRSQA
                     LKLFSGFPYAVAPDEAPAAESAPEAAAAKPKRAPRAAARKPRAKKK"
     gene            5339..6676
                     /gene="rimO"
                     /locus_tag="VFV55_00700"
     CDS             5339..6676
                     /gene="rimO"
                     /locus_tag="VFV55_00700"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX4942837.1"
                     /translation="MASRARKAPARAPRVGFVSLGCPKALVDSESILTQLRAEGYDIA
                     PSYEGSDLVVVNTCGFIDAAVEESLDAIGEALAANGKVIVTGCLGAKGDVVRNAHPKV
                     LAVTGPHATPEVMAAVHAHLPRPHDPFADLVPPQGIRLTPRHYAYLKISEGCNHRCTF
                     CIIPSMRGDLVSRPVGEVMKEAESLVNAGVKELLVISQDTSAYGVDVKYRTGFWGGRP
                     LKTRMTQLCAALGGLGVWVRLHYVYPYPHVDEVIALMAGGRILPYLDVPFQHASPRIL
                     RLMKRPAAAEKNLERIRAWRDGCPDLTIRSTFIVGFPGETEAEFGELLDFLREARLDR
                     VGCFAYSPVEGAKANELPGAVPEEAKQERLARFMEVQATVSRGRLAAKVGREIDVLVD
                     DVEGRVAIARSSADAPEIDGVVRVKGAADVRAGDFARVRVTAAGEHDLEAVPA"
     gene            complement(6681..8489)
                     /locus_tag="VFV55_00705"
     CDS             complement(6681..8489)
                     /locus_tag="VFV55_00705"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4942838.1"
                     /translation="MAERLRTIRSLGPWLAPLIGAGAVLLLVLVAFAEWRALRGFGEL
                     NVATERQRELLEDLASVRALENAMIEMESGMRGFVLANRDDFLEPFTRGQEEFAAIAR
                     DLRERWRGTPDQAGMLESIVALESDWRNAFADPLVERRRATRTSADWGTIEETVARGD
                     GKRRVDAVRALASQLETQVAARIRAVSAEIGRRSQIARNFAIRTGLAVLLGIGFMAAV
                     IARGFRTAAEQNAALSREIADRQQAELRARASESLFEAFLNGIADALVIVDDNGRIAW
                     TNPRLPALFGFAPDELQGAAVTRLLADADHPRLEEKLAVLRGGAQERIEIEVTARSAN
                     GREFAAELSLGRFDAPQGFRTALVIRDVSERKAVERLKSEFIASVSHELRTPLTAIVG
                     SLSLLRSGEAGEMEAGARDFVEMAHENSLRLAQLVDDVIDVERIGSGALAFREARFPL
                     PEFLAEAVRLNQGYAEAHGVFFFLDEPVPQASLRGDRGRLMQALTNLLSNAAKFSPRG
                     EEVHVSARLAGTRVRIAVTDRGPGIPEEFRPHVFEKFAQADGSDSREKGGTGLGLAIA
                     HAIVMRLGGSIGFETSTGKGTTFWIEMPVTDEATGG"
     gene            complement(8474..9874)
                     /locus_tag="VFV55_00710"
     CDS             complement(8474..9874)
                     /locus_tag="VFV55_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4942839.1"
                     /translation="MNAVADALQWVFLAYFVGVNLVYVGLNLLAIPTLRRYLAIRPIE
                     NLPPVYSGFEPPVSVLVPAYNEESTIVTSVRSLLQLDYPEFEVVVVNDGSRDATLDRL
                     REAFDLEPFPEARWERVPGKPVRAVYASRRHASLRVVDKDNGGKADALNVGINASRYP
                     LFCAIDADSILQRDSLRRVVQPFLDDPRTVATGGTVRIANGCTVSGGQLERVDLPANY
                     LALLQIVEYLRAFLFGRLGWATLDAVLIISGAFGVFRKDAVVAAGGYRTDTMGEDMEL
                     VVRIHRLFRLAGEPYAIHFVPDPICWTEAPEALAVLKSQRMRWQRGLAESLSLNRALL
                     ASPRGGAPGHLAFPFFLVFECWGPVIEVAGYAFMAALAATGRISGEAFLAFLALAFAM
                     GFLLSVSALLLEEVSFHLYPRFSHVARLILAAVAENLGYRQLVTVWRLVGLVRWLRGA
                     RAQWGTMTRKGSWQNG"
     gene            complement(9871..10875)
                     /locus_tag="VFV55_00715"
     CDS             complement(9871..10875)
                     /locus_tag="VFV55_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX4942840.1"
                     /translation="MAALASAVVLVIALVVLRLERGIRVRRLAAHIARWRMALHVAME
                     SPGEATLPDIAPVDLPVFLQLWNHLQESLRGEASDNLARVVREKGLVPAIRELAIRRA
                     LRHRLVAITALGHLREAAAWELLASLARDPDPVLSFAAVRALLRIDARRALEDHLADA
                     VHRADWSLARLGTALQEAGPDEVTPPMERVLADPPADGLERLLKLAKFAHRERVSAMI
                     HGWLGASAKAHVIAAALDFVEDAADLPRVRGATRHGAWHVRMSAARALGRIGGERELA
                     LLLAMLDDPSWWVRYHAAQAIVALPGLAQRDLEQFRARAPSGVAAAMLAQVLAERSRA
                     "
     gene            complement(10907..11659)
                     /locus_tag="VFV55_00720"
     CDS             complement(10907..11659)
                     /locus_tag="VFV55_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEX4942841.1"
                     /translation="MNRAASCLVALAIAPAAAADFPRNEARLFGAHENLDNGFADWRE
                     AGAEFQWQAARDRGAFLRVRETERYGERDREAAAGFSAPVAPGWVLAFEGSGAPSPQV
                     LPEWSALATLSRVFGEGWVASAAARRTRYATSSVSGAIAGLERYAGSFRLAWSLFLSR
                     PEHAAWSSTHRLAATWYGEGLTRAEAGFARGRESEFVPGTGLATSDVRSATLSASLGL
                     APAWALTLDLERQRQGNRYTRETIRLGTRLLF"
     gene            complement(11656..12036)
                     /locus_tag="VFV55_00725"
     CDS             complement(11656..12036)
                     /locus_tag="VFV55_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4942842.1"
                     /translation="MPATRSALVVEDDPDIGHLLRFILEREGFAVTHLSDGRAALALV
                     AGGAPPDLALLDLMLPHATGHDVLAAMRAQAAWRTVPVIMLTAKSHEADIVRALDGGA
                     NDYIVKPFQPAELKARIRRLVPAP"
     gene            complement(12042..12614)
                     /locus_tag="VFV55_00730"
     CDS             complement(12042..12614)
                     /locus_tag="VFV55_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4942843.1"
                     /translation="MSSTPGPAHPEDPYDLDFTSLVEEPSAPPVPPAVPTPEHEEIAL
                     IGANALGEDGFHVVLVRPRVGSSSNQVIMIVDDDEPTAELAALVLRKAGYRTVVALGA
                     RDAARLMSRLGAPELLLLDVEMPEMGGLEFLARIRRHKRLSTTPVVLFTAHSSQDDIM
                     RGLQAGADGYIAKPIAASALVSAVETVLGH"
     gene            complement(12668..13090)
                     /locus_tag="VFV55_00735"
     CDS             complement(12668..13090)
                     /locus_tag="VFV55_00735"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX4942844.1"
                     /translation="MKITGRQLSAAVSTALFAAAIAVAPAAEAKCDNCGTVTGVKTIK
                     KEGEGSGVGAIAGGVLGGVLGHQVGSGRGNTAATIVGAGAGAYAGNQVEKSRKSTTTY
                     QVVVKLEDGKSRTFNFSSETSYRAGDKVKIVDGKLTRQ"
     gene            complement(13151..13555)
                     /locus_tag="VFV55_00740"
     CDS             complement(13151..13555)
                     /locus_tag="VFV55_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4942845.1"
                     /translation="MSKVPFQRILHVDDQPDIRGIVKLALGKIGGFSVCSCSSGQEAL
                     AAATEFDPELLLIDMSMPGMDGMDLLLRMREAGIHVPAIFFTARINPAELERYRAAGA
                     IGTVSKPFDPLKLGRQITQIWVEHHAPKESPN"
     gene            complement(13561..15513)
                     /locus_tag="VFV55_00745"
     CDS             complement(13561..15513)
                     /locus_tag="VFV55_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4942846.1"
                     /translation="MNDFPVSFVDAFLLAILPVAAFLMGVALVMLRRRRAAHLATMAD
                     LDAERDRVRNALAGSSLTIWDWDLAADSVWLNANWKEMLGESAGETRCTIADLAALVH
                     PDDLERVQKTAIAAFKGIDAQLDAEFRVKCADRSWRWIRTRGKVIARDAKGRAVHVSG
                     TNADVTPRKLTEKSLAESEARFRALSELSSDLYWEQDEHFNLVSWSAPAWARGLDSML
                     PRPALDAPELTEADWGSHRAVLEERRAFRDLEIRQVSPDGRESWLLVGAVPVVDERGR
                     FRGYRGLARIITDRKQAEQRLRKSESQFRQLVEFMPAAIVLVSPDRRVVVHNKAYAEL
                     HGLPGVGIDGRQVREIVGDERFAEIEPHLRRALQGEPAHYATQRTVPGGRIVDLDVLL
                     WPLRGADGEVEGVIELGIDVTRLKEADRMKDHFVSVVSHELRTPLTSMRGSLGLLAGG
                     VVGDLPSEAKPLVDIALQNSDRLWRLVNDLLDIEKMSAGYVEFHVAAIDWRAALREAV
                     ESSRGLMQEYDVSFELEPVADLHVLGDTDRLSQVLSNLILNAAKFSPAGGVVSVGAQR
                     LTGGGVHTTVRDRGPGIPENFRSRVFRPFSQADSRDSRSTGGSGLGLAISREIVTRLG
                     GTIGFDDEPGGGTTFWFDLPEAAVAQ"
     gene            complement(15520..15834)
                     /locus_tag="VFV55_00750"
     CDS             complement(15520..15834)
                     /locus_tag="VFV55_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4942847.1"
                     /translation="MTDPKPDKVPLDISDEVRELAGLFLRERHADLARLDAALADDDY
                     ATIRAVGHAFRGSSAPFGFPEAGRIGAELEKAAERHDRAAVDALVPRLRAAIPQAGPG
                     EP"
     gene            complement(15857..>16721)
                     /locus_tag="VFV55_00755"
     CDS             complement(15857..>16721)
                     /locus_tag="VFV55_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX4942848.1"
                     /translation="KPFTTHHNALDMDMFLRIAPELYLKKLVVGGLEKVFEINRNFRN
                     EGISTRHNPEFTMIEFYEAYRDYHYLMDLTEGLLRECAQKVVGTTTLAYQGETIDLAK
                     PFDRLTMAEAIAKYNPQYPLGELSRAEYLKVALAPFEVEVFPTDGLGLLQLKLFEATT
                     EARLVQPTFIVAHPTDVSPLARANDANPAVTDRFELFITGREMANGFSELNDPEDQAA
                     RFAGQAAAKEAGDEEAMYYDADYIRALEYGLPPTAGEGIGIDRLAMLFTDSPSIRDVI
                     LFPQLKSADTG"
BASE COUNT         2589 a         5614 c         5948 g         2570 t
ORIGIN      
        1 gcccaaggac gccgatagac ttgccccatc cccggcccac atgaataggc agcgtggcgc
       61 attcagcaac gatggcgccg cggtccaatg ttgcctcact cgctgctgta tccaccgcct
      121 cctcgacttg cgcacccagg attgcgatcg acttccaccg ccaccgcctc ctcgacttgc
      181 gcacccagga ttgcgatcga cttccaccgc caccgcctct tcagcttgcg tgcccgggat
      241 tgcgatcgac ttccaccgcc accgcctcct cagcttgcgt gcccgggatt gcgcttgctt
      301 cccgattgac gggccgtggt ccggcgggta ttcagggcag gtcggagagc ggcaccggcc
      361 gggagatgcc gaatccctgc gcgaagtcca cgccgtactc gcgcagcttg gcgagcgttt
      421 cctcgttctc gacgaattcg gcgatcgtga ggcggttcgt cgcgcgcgcc acgcgcgtga
      481 tcgccttgag cttgatcagg tccacggagt tgcgcagcag gcccaggatg atgtcgccgt
      541 cgatcttcag gacatcgacg cgcaggcgct tgaggagggc gaaggagtcg gcattctgcc
      601 cgaagccgca cagcgcggtg cggcaccctg cctcgccgat taccgagatg aagcggacgg
      661 tgtcccgggg gcgggcgacc gcatccgact ccgtgatctc gaaacacagg agcgaaccct
      721 tcagcccgcg ctggtgcagc gcggcgacca cgaaatccgg gaaatcggcg ttgccgatcg
      781 tcgggccgga cacgttcagg ctgtacgtgg ccgcctgcct gcggggactt gcgccggccc
      841 agtcgaggac attcttcacg acccatcggt cgagatcggg gagcaggccg tactcctcgg
      901 caaggggaag gaacgcgccc ggcggcatga ggccctcttc ttcctcgcga aggcgcagga
      961 gtatctcgta atacggctcg ccctgcgctc ccgcggcgac gggcacgatg gcctgggtgt
     1021 acagcgagaa ttcgttgccg tcgagcgcgg cgcgcaggcg gtcgcccgcg ttcttccagt
     1081 cggtgagctc ctcggtcatg gtgttcagga agatgcgccg ctcgtgctcc gcgccgttcg
     1141 gtgcgggcgg tgacgacggc ggaatctcag aagccgtgcc cgggacggta tcgggcgggg
     1201 gagccgccac ggaagccggc gtggccaccg atggcgcctc cacggacgag atgtcagtct
     1261 ggacgacgaa tgcgccgaca atttccccct ttgccccgac gtggggcagg tactgcgtgg
     1321 agagccgcga ttcgggactg cccaggccct cgtggatcct tgtttcatgc ttcgcgcgcc
     1381 ccgccagtgc ggaatcgatg ccggctttca tctggccgaa ggtcgtcagg ccgacgacgc
     1441 tgcgcagcgg gcgcccgttg atcgaatcgc gcctggcgcg cagccagtag cggaacgcct
     1501 ggttgtggaa tcgaaccgtg ccatccgtgg tgacgtacac gatcatgacc ggcagttcgt
     1561 cgtcgatgta gcggacgctc tcccggacct cggcgagggc agtctccgcg taggcgagcc
     1621 ggctcaccag gccgatggca cccgcgctga gcatgccggt gaggaacgcg atccagggca
     1681 ggtcgaacag cgtgaagaag atggtcgtga cgaggagggt cccgatgacc atcatgccgg
     1741 ccacgccggc cagcgggttg atgcggtcga agacggctcg aattcgcatg accggtattg
     1801 tctcctgatc gaggcgcgac gggaacactg gccgcgcaag ggtgcccgcc gcaggccggg
     1861 ggtacaatcc gctcttcatc gggagtcccc gttacgaggt cctgccgggt caccggaagc
     1921 gcgaatcgaa ggtgtcgccc acgacgcatg agcaggcgag cggatccccg gtccccggcc
     1981 tcgagggctg gccggttccc aatcactttt ccatcgtctc gatcgcgcac gggggcgaaa
     2041 ccgaacgcgc gaggatcgag atgggcgacg gccgcgagct gaccggcgat gccgttcggc
     2101 tcgacagcgt ggccacgatg ctcgaattcc gcccggacgg cgcgccggca agccagttca
     2161 tccgcttctc gtcgttcaag aagctgtgcc tcacccgtcc cgtcgagctg gaacgcctgc
     2221 gcctggcgat tcccccggaa aacgtggacg cctatccatc gcacgcgggg ctcaacttca
     2281 cgatccgctt caaggacggc tccgagtttt cctctccgat ccttggcctc gtcgcgcgca
     2341 ggatcgggct cttcccctac ctcaccggcg agcccgggag cgtcctgcgc tggttcatgc
     2401 cggccgaggc gacgtcgggt tgccggatcg gcgacgccct cggcaagatc ctcgtcgagc
     2461 gcgcggtcgt gtcgtcggac atcgtcgagg cgggcctcga aaggcagcgc cggcagcgcg
     2521 cgacgaagtt cggcgagttc ctttgccggc agggcatcgt gacccgcgag caggtggaag
     2581 cctgcctcct ggcccagtcg ggccagcgcc acctgaagct gggcgaagtg ctcgtcgcgc
     2641 gcggctacgt cacggcggaa cagcgcgacc aggccctcgc ggaccaggcg gtggaacgcg
     2701 gaatgccgct cggcgagata ctggtgcaga tgggagcggt cgcgcggaag gttctgcggc
     2761 aggcgcttgc cgaccagttc gggctaccct gggtgagcct gcgcgggttc aggttcgaac
     2821 gcgaggcgat cgacgcgatt tccccggagg cggcgcgaag gcaccttgtg gtgccgctgt
     2881 atcgctccgg ggtgcgcatc gtcctggccc tgagcaatcc cgtggactgg gaggccgtcg
     2941 aggagatcga gtccgcgtcg ggctcgagag tcgatccggt gctctcctcg cgggaggact
     3001 tgcgcgaggc gatcgcgcgc cactacggcc cgggctagcc ggggccgcgc cgctcaggcg
     3061 tacatgtgca ggccgccgtt caccgagagg ttggtgccgg tgatgtagga ggccttgtcg
     3121 gaggcgagga aggcaacgga gtcggccacc tcgtcgggca tcgcgaggcg cccggcgggg
     3181 atctgcgcga cgatctgctt gcgcacgtct tcgcggatgg ccgcgaccat ctcggtttcg
     3241 acgtagccgg gagagatcgc gttcacggtc acgcccttgc gcaccacttc ctgcgcgagc
     3301 gccttggtga agccgagaat gcctgccttg gcggccgagt agttggtctg cccgaactgg
     3361 ccgcggaccg cgttgaccga cgagatgttg atgacgcggc cccatccgcg gtcgcacatg
     3421 ccgtcgatca cgtggcgcgt cacgttgaag acgctggtga ggttggtctc gatgaccagg
     3481 tgccactgct cgggcgtcat cttgcggaaa gtcgagtcgc gcgtgatgcc ggcgcagttg
     3541 accaggatat cgaccgggcc gtgttccctg gcgatattgg cgaccatctc gcccatcgcg
     3601 tcgtaatccg acacgtcgcc gaaggcgagc gccgtgcttc cgccgttgag gcccgcagcc
     3661 gccatgttcg cgaggtactt gctctccgag cccgggatgg cgtagttggc gacgacgaaa
     3721 ttaccctgct gcgcgagacg cttgcagatg gcagagccga tgccgccgac gccgccggtt
     3781 acgagtgcga tcttcttgtc catgatgcag ccttccacgt gacgggcgcc gctcatcgcg
     3841 ggcaccctct aaaaaaaaac cccagggatg aacctggggt ttgaaccttt atccaaattg
     3901 gagaatttgc gataaaggag gaggagacga tggcggaacg cgtccgcttt acttgcgccc
     3961 cttgggcgcc gtggcggcct tcacgcccgc gtcggtgaag ctcgcggcat gacgggcggc
     4021 cttggaaaac gaatcgaagg cggcggtggc ggcggcgagc gaggatttca tcgccgcgac
     4081 ggcgacgtcg ctgccggccg gcgcggactt ggcggcctgc tcgacggtgt cggtgacgtt
     4141 gcgctgcagt tccgccatgc gctcctcggc gagcttcgac agttcggact gcgcgtccga
     4201 cgccacctgg taaaggccgc gcgagtattc gagccactgg ctcatggcgg tctcggctgc
     4261 cttggtgcgc agcgcaagca gttgctgcac gtcgttggag ccggccgcgg cacgggcggt
     4321 gcgggtcgcg tcgcccaggg acgtcttggc gaaggcgagc tgcagcgcga gcagccgctc
     4381 ggcgctatcc agcgacagct tcgacaggcg cgccgccgaa agcacggcgg tgttgttcag
     4441 ttcgttcatc tgctcggcga ggttaaccat ggtcgtctgg tcccgtaatg agggcggcgt
     4501 cgggaaattg tgcgccgcaa caattcaatt ctaggaggga gacgcgcgcc tgtcaaggcg
     4561 atattattgc gctgcaccac aagcgggcgc cgcactgccg caatccgcta aactgtaata
     4621 aaatgaagta cttggtgcgt atccggagag aatggtgccg gcgccgcaca atccccgatt
     4681 gatgtagcgc aacattccta ggacccctgc catggcttcg ccccgcatca tcaagaagta
     4741 cccgaaccgc aggctctacg acacggagac ctccacctac atcacgctcg cggaggtgaa
     4801 ggacctggtc ctgcagtaca tggatttcca ggtgcaggac gcgaagtcgg gcgaggacct
     4861 gacgcgcgcg atcctcctgc agatcatcct cgaggaggag tctgggggag gcccgatgtt
     4921 ctccaccgac atgctctcga acatcatccg ctactacggc cactccatgc aggggctcat
     4981 gggctcgtac ctcgagcgct cgatacacgc cttccacgag gcgcagaagc gcttccaggc
     5041 gcaggcccag gtcctcatcc cgaaggtgcc cgccgatgcg ttcgcgaaca tgatggcggg
     5101 cgggcagccg atcccgcagg tgatgggcgg gtacctcgag aagggcgcga aggcggtgat
     5161 cgacatgcag gaggaactgc gcagccaggc cctgaagctc ttctccggct tcccctatgc
     5221 cgtcgcgccc gacgaggcgc cggccgcaga gtctgcgccc gaggccgccg ccgcgaaacc
     5281 gaagcgcgcc ccgcgcgcag ccgcgcgcaa gccgcgcgcg aagaagaagt agggtctctt
     5341 ggcgtcgcgt gcccgcaagg cgcccgcgcg cgcgccgcgc gtgggcttcg tgtccctggg
     5401 ctgtcccaag gcgctggtcg attccgaatc catcctcacg cagctgcgcg ccgagggcta
     5461 cgacatcgcg ccctcgtacg agggctccga cctggtggtg gtgaacacct gcggcttcat
     5521 cgacgcggcc gtggaggagt cgctggacgc gatcggcgag gcgctggccg ccaacggcaa
     5581 ggtgatcgtg accggctgcc tcggcgccaa gggcgacgtc gtccggaatg cgcacccgaa
     5641 ggtgcttgcc gtgaccgggc cgcacgccac gcccgaagtg atggccgccg tgcacgccca
     5701 tcttcccagg ccgcacgacc cgttcgccga cctcgtgccg ccgcagggca ttcgcctcac
     5761 gccgcgccac tacgcctatc tcaagatctc cgagggctgc aaccaccgct gcaccttctg
     5821 catcatcccg tcgatgcgcg gggatctcgt gagccgcccg gtgggcgagg tcatgaagga
     5881 ggccgagagc ctcgtgaatg ccggggtgaa ggaacttctc gtcatctcgc aggacaccag
     5941 cgcctacggc gtcgacgtga agtaccgcac cggcttctgg ggcggcaggc cgctgaagac
     6001 gcgaatgacg caactgtgcg cggcgctggg cgggctgggc gtgtgggtgc ggctgcacta
     6061 cgtctacccg tacccgcacg tcgacgaggt gatcgctctc atggccgggg gacggatcct
     6121 gccgtacctc gacgtcccgt tccagcacgc gagcccgcgc atcctgcgcc tcatgaagcg
     6181 gccggcggcg gccgagaaga acctcgagcg catccgcgcg tggcgcgacg gctgccccga
     6241 cctcaccatc cgctccacct tcatcgtggg ctttcccggg gagaccgagg cggagttcgg
     6301 ggagctgctc gatttcctgc gcgaggcgcg tctcgatcgc gtgggatgct tcgcctattc
     6361 gccggtggaa ggcgcgaagg ccaacgagct tccgggcgcc gtgcccgagg aggcgaagca
     6421 ggagcggctc gcgcgcttca tggaagtgca ggccacggtg agccgcgggc ggctggccgc
     6481 caaggtgggg cgcgagatcg acgtgctcgt cgacgacgtc gagggccgcg tcgcgatcgc
     6541 ccgcagcagc gccgacgcgc cggagatcga tggggtggtg cgcgtgaagg gcgccgcgga
     6601 cgtgcgggcc ggcgatttcg cgcgcgttcg cgtcacggcg gccggcgagc acgacctcga
     6661 ggccgttccg gcctgaggtg tcagccgccg gtcgcttcgt cggtgaccgg catctcgatc
     6721 cagaacgtcg ttcccttgcc cgtcgaagtc tcgaacccga tgctgccgcc caggcgcatg
     6781 acgatggcgt gcgcgatggc cagccccagg cccgtgccgc ccttctcgcg cgagtcggac
     6841 ccgtcggcct gcgcgaattt ctcgaacacg tgcgggcgga attcctcggg gatgccgggg
     6901 ccgcgatcgg tcaccgcgat gcgcaccctc gtgcccgcga gccgcgcgga cacgtgcact
     6961 tcctcgccgc gcggcgagaa cttggccgcg ttcgagagca ggttcgtcag tgcctgcatg
     7021 agccgcccgc ggtcgcccct cagcgacgct tgcggcaccg gctcgtcgag gaagaagaag
     7081 acgccgtgcg cctcggcgta gccctggttc aggcgcaccg cctccgcgag gaattccggc
     7141 agcgggaagc gcgcctcgcg gaaggcgagc gccccggagc cgatgcgctc gacgtcgatc
     7201 acgtcgtcga cgagttgcgc gagccgcagg ctgttctcgt gcgccatctc gacgaagtcg
     7261 cgcgcgccgg cctccatctc gccggcctcg ccggaacgca gcagcgacag cgagcccacg
     7321 atggcggtga gcggcgtgcg caactcgtgg ctcaccgagg cgatgaactc gctcttcagg
     7381 cgctcgacgg ccttgcgctc ggagacgtcg cggatcacca gcgcggtgcg gaagccctgg
     7441 ggcgcgtcga agcggcccag ggacagctcc gcggcgaact cgcggccgtt cgcggatcgc
     7501 gccgtcacct cgatctcgat gcgctcctgc gccccgccgc gaaggacggc cagcttttcc
     7561 tcgaggcgcg ggtggtcggc gtccgcgagc agcctcgtca cggccgcgcc ctggagttcg
     7621 tctggcgcga agccgaaaag cgccggcagc cgggggttgg tccaggcgat gcggccgttg
     7681 tcgtccacga tcaccagcgc gtcggcgatc ccgttcagga acgcctcgaa gagcgactcg
     7741 ctcgcccggg cgcgcagctc cgcctgctgc cggtcggcaa tctcgcgcga cagggcggcg
     7801 ttctgctccg ccgcggtgcg gaagccgcgc gcgatgacgg ccgccatgaa cccgatcccg
     7861 agcagcaccg cgagcccggt gcggatcgcg aagttgcgtg cgatctggga gcgccggccg
     7921 atctcggcgg aaacggcgcg gatccgcgcg gcgacctgcg tctcgagttg actggcgagc
     7981 gcgcgcaccg cgtccacgcg gcgcttgcca tcgccgcgcg cgacggtctc ctcgatggtc
     8041 ccccagtcgg ccgaagtccg cgtggcgcgc cggcgctcca ccagcggatc ggcgaatgcg
     8101 ttgcgccagt cggactcgag ggcgacgata ctctcgagca tgccggcctg gtccggcgtg
     8161 ccgcgccatc gttcgcgcag gtcgcgcgcg atggcggcga actcctcttg cccccgcgtg
     8221 aacggctcga ggaagtcatc ccggttcgcg aggacgaagc cgcgcatgcc cgactccatc
     8281 tcgatcatcg cattctcgag cgcacgcacg ctcgcgagat cttccaggag ctcgcgttgc
     8341 cgttcggtgg cgacattgag ctcgccgaag ccgcgcaggg ccctccattc ggcgaacgcg
     8401 acaagcacca gcagcaggac cgcaccggcg ccgatgagcg gcgcgagcca cgggccgagc
     8461 gagcggatcg tcctcagccg ttctgccatg agcccttccg cgtcatggtg ccccattgcg
     8521 ccctggcccc gcgcagccag cgcacgaggc ccacgagccg ccagacggtc acgagctggc
     8581 ggtagccgag gttctcggcc acggcggcga ggatcagccg cgcgacgtgc gagaagcgcg
     8641 ggtagaggtg gaaggagact tcctcgagca gcagcgcgct gaccgagagc aggaatccca
     8701 tcgcgaaggc gagcgcaagg aacgcgagga aggcttcgcc cgagattcgt cccgtcgcgg
     8761 cgagcgcggc catgaaggcg tagcccgcga cctcgatgac cggcccccag cactcgaaga
     8821 ccaggaagaa cgggaaggcg aggtgcccgg gggcgccgcc gcgcggggag gcgaggagcg
     8881 cgcggttgag ggaaaggctc tcggcgagtc cgcgctgcca gcgcatgcgc tggctcttca
     8941 gcaccgcgag cgcctccggg gcctccgtcc agcagatggg gtcgggcacg aaatggatgg
     9001 cgtagggctc ccccgccagg cggaacagcc ggtggatacg caccaccagt tccatgtcct
     9061 cgcccatcgt gtcggtgcgg tagccgcccg cggcgacgac cgcatccttg cggaacacgc
     9121 cgaaggctcc cgagatgatg agcaccgcat cgagcgtggc ccagccgagc cggccgaaga
     9181 ggaacgcgcg caggtactcg acgatctgca ggagcgcgag gtagttcgcc ggcaggtcga
     9241 cgcgctcgag ctggccgccg ctcaccgtgc acccgttggc gatgcgcacg gttccgcccg
     9301 tggccaccgt cctcgggtcg tcgaggaagg gctggaccac gcgtcgcagg ctgtcgcgtt
     9361 gcaggatcga gtccgcgtcg atcgcgcaga agagcgggta gcgcgaggcg ttgatgccga
     9421 cattgagcgc atcggccttt ccgccgttgt ccttgtccac cacgcgcagg ctggcgtgcc
     9481 ggcgcgacgc gtacaccgcg cgcacgggct tgcccggaac gcgctcccat cgcgcttccg
     9541 gaaagggctc gaggtcgaac gcctcgcgca gccggtccag ggtcgcatcg cgcgagccgt
     9601 cattgaccac gaccacctcg aactccggat agtcgagctg cagcagcgag cgcaccgacg
     9661 tgacgatggt cgattcctcg ttgtaggcgg gcaccaggac cgacacgggc ggctcgaatc
     9721 cggaatagac gggcggaagg ttctcgatcg gccgtatggc gaggtagcgc cgcagcgtcg
     9781 ggatcgcgag caggttgagc cccacgtaga cgaggttgac gcccacgaaa taggcgagga
     9841 acacccactg cagcgcgtcg gcgacggcgt tcacgcgcgc gacctttcgg ccaggacttg
     9901 cgcgagcatc gccgcggcga cgccgctggg cgcgcgggcg cggaattgct cgaggtcgcg
     9961 ctgcgcgagc ccgggcaacg cgacgatggc ctgcgccgcg tgatagcgca cccaccacga
    10021 cgggtcgtcc agcatcgcca gcagcagggc cagctcgcgt tcgccgccga tccgcccgag
    10081 cgcgcgggcg gcggacatgc gcacgtgcca cgcgccatgc cgcgtcgcgc cgcgcacgcg
    10141 cggaaggtcg gcggcgtcct ccacgaaatc gagcgccgcg gcgatcacgt gcgccttcgc
    10201 gctcgccccc agccagccgt ggatcatggc cgacacccgt tcgcggtggg caaatttggc
    10261 gagcttgagc aggcgctcga gcccgtcggc gggcgggtcg gcgagcacgc gctccatcgg
    10321 cggcgtcacc tcgtccggtc ccgcttcctg cagcgcggtg cccaggcgtg cgagggacca
    10381 gtccgcgcgg tgcacggcgt cggcgaggtg gtcctcgagg gcgcggcggg catcgatgcg
    10441 caggagcgcg cgcacggcgg cgaaggaaag cacggggtcg ggatcgcgcg cgaggctggc
    10501 gagcagttcc cacgcggccg cttcgcgcag gtgccccagc gcggtgatag cgaccaggcg
    10561 gtggcgaagc gcgcgccgga tggcgagttc gcgtatcgct ggcacgaggc ccttctcgcg
    10621 caccacgcgc gcaaggttgt ccgatgcctc gccgcgcaac gactcctgaa ggtggttcca
    10681 gagctgcagg aacaccggca ggtcgacggg cgcgatgtcc ggcagggtgg cttcgccggg
    10741 cgattccatc gccacgtgca gcgccatcct ccagcgggcg atgtgcgcgg caaggcggcg
    10801 gacgcgaatg cccctttcga gtcgcaggac caccagcgcg atcaccagga caactgccga
    10861 ggcgagcgcg gccatggcgg cgccttcggc gagagcaagg ggaaagctag aagagtaggc
    10921 gggtgccaag gcggatggtc tctcgcgtgt agcgattgcc ctgccgctgg cgctcgaggt
    10981 cgagggtgag ggcccaggcg ggagcgaggc cgaggctcgc ggagagggtc gcgctgcgca
    11041 cgtcgctcgt ggcgagcccg gtgccgggca cgaactccga ctcgcggccg cgcgcgaacc
    11101 ctgcctcggc gcgcgtgagc ccttcgccgt accaggtcgc cgcgaggcgg tgggtggacg
    11161 accacgcggc gtgctcgggt cgcgagagga acagggacca cgcgaggcgg aagctgccgg
    11221 catagcgctc cagcccggcg atggcgccgg agacggagga agtcgcatag cgcgttcgcc
    11281 tggcggccgc cgaggccacc cagccttcgc cgaagacgcg cgaaagcgtg gcgagcgcgg
    11341 accattcggg aagcacctgc ggcgagggcg cgccgctccc ctcgaaggcg aggacccagc
    11401 ccggcgcgac aggcgccgag aaacccgcgg ccgcttcgcg gtcgcgttcg ccatagcgtt
    11461 cggtttcgcg cacgcgcagg aatgcgccgc ggtcgcgcgc cgcctgccac tggaactccg
    11521 caccggcttc gcgccagtcc gcgaagccgt tgtcgaggtt ctcgtgcgct ccgaagagcc
    11581 gcgcctcgtt gcgcgggaag tcggcggccg cggcaggggc gatggcgagc gcgacgaggc
    11641 aggacgcggc gcggttcatg gcgcgggaac cagccggcga atgcgcgcct tgagctccgc
    11701 gggctggaac ggcttcacga tgtagtcgtt ggcgccgccg tcgagggcgc ggacgatgtc
    11761 ggcctcgtgg gacttggcgg tgagcatgat gaccggaacc gtccgccacg cggcctgcgc
    11821 ccgcatcgcg gcgaggacgt catggccggt ggcgtgcgga agcatcaggt cgagcagcgc
    11881 gaggtcgggc ggcgcgcccc ccgccacgag agcgagcgcc gcgcggccat cgctcaggtg
    11941 cgtcactgcg aacccttccc gttccaggat gaagcgcagg aggtggccga tgtcggggtc
    12001 gtcctcgacc acgagggcgg accgggtggc tggcatcggc gtcagtgccc cagcacggtc
    12061 tcgacggccg aaaccagcgc ggaggcggca atcggcttcg cgatgtagcc gtctgcgccg
    12121 gcctggagcc cgcgcatgat gtcgtcctgg ctgctgtggg cggtgaagag cacgacgggc
    12181 gtggtggaaa ggcgcttgtg ccgccgtatg cgcgcgagga actcgagccc acccatctcc
    12241 ggcatctcca cgtcgaggag caggagttcc ggcgcgccca gccgcgacat gaggcgggcg
    12301 gcatcgcgcg ctcccagcgc gaccacggtc cggtaacccg ccttgcgcag caccagcgcc
    12361 gcgagctcgg cggtgggctc gtcgtcgtcc acgatcatga tgacctggtt ggacgagctt
    12421 cccacccgcg ggcggacgag cacgacatgg aagccatcct cgccgagcgc gttcgccccg
    12481 atgagcgcga tctcctcgtg ctcgggcgtc gggaccgcag gggggaccgg cggggcggaa
    12541 ggctcttcga cgagcgacgt gaagtcgagg tcgtagggat cctcgggatg ggccggcccg
    12601 ggcgtgctag acattttcca tcacgatcgg gttggccgcc gcaatggggc gggccgcccc
    12661 ggcgcggcta ctggcgcgtg agcttgccgt cgacaatctt gaccttgtcg cccgcgcgat
    12721 agctcgtttc cgaggagaag ttgaaggtgc gcgactttcc gtcctcgagc ttcacgacga
    12781 cctggtaggt ggtggtcgac ttgcggctct tctcgacctg gttgcccgcg taggcgcctg
    12841 cgccggcgcc gacgatcgtc gcggccgtgt tgccgcgccc gctgccgacc tggtgaccga
    12901 gcacgccgcc cagcacgccg ccggcgatgg cgccgacgcc cgagccttca ccttccttct
    12961 tgatcgtctt cacgccggtg acggtgccgc agttgtcgca tttcgcctcg gcggccggcg
    13021 cgacggcgat ggcggctgcg aagagcgccg tcgaaacggc ggcggagagt tggcgaccgg
    13081 tgatcttcat gacgtgttcc tccgtgatgc tgccgggata tggcgaaatg ttagcacgcg
    13141 gctcgggagc tcaattcggg ctctccttcg gggcatggtg ctccacccag atctgggtga
    13201 tctgccggcc gagcttgagc gggtcgaacg gcttggagac ggtgccgatc gcgccggctg
    13261 cgcggtagcg ttcgagctcg gccggattta tccgggccgt gaagaagatc gccggcacgt
    13321 gaatgccggc ttcgcgcatg cgcaggagga gatccatccc gtccatgccg ggcatgctca
    13381 tgtcgatgag gagcagctcc gggtcgaatt ccgtcgcggc cgcaagggcc tcctgccccg
    13441 agctgcaact gcacacggag aagccgccga tcttcccgag ggcaagcttg acgatcccgc
    13501 ggatgtcggg ttgatcatcg acgtgcagga tgcgctggaa cggcactttc gacatgggac
    13561 tcattgtgcc accgccgcct cgggaaggtc gaaccagaaa gtcgtgccgc cgccaggctc
    13621 gtcgtcgaac ccgatcgtgc caccgagccg ggtgacgatc tcgcggctga tcgcgaggcc
    13681 gagccccgaa ccgcccgtcg aacggctgtc gcgggagtcg gcctgcgaga aaggccggaa
    13741 gacccgcgag cggaagttct cggggatgcc cggaccgcgg tcgcgcacgg tcgtgtgcac
    13801 gccgccaccg gtcaggcgtt gcgcgcccac ggacacgacg ccgcccgccg gcgagaactt
    13861 ggccgcgttg aggatcaggt tcgacagcac ctgcgaaagc cggtcggtgt cgcccaggac
    13921 gtgcaggtcg gccacgggct cgagctcgaa ggagacgtcg tactcctgca tcagcccgcg
    13981 gctcgactcc acggcttccc gcagggccgc ccgccagtcg attgccgcga cgtggaactc
    14041 gacgtaaccc gccgacatct tctcgatgtc gaggaggtcg ttcaccaggc gccagaggcg
    14101 gtccgaattc tgcagcgcga tgtccaccag gggcttggct tccgacggca ggtcgcccac
    14161 gacgcctccg gccagcagcc cgagggagcc gcgcatggag gtgaggggcg tgcgcaactc
    14221 gtggctcacg accgatacga agtggtcctt catgcggtcg gcctccttga ggcgcgtgac
    14281 atcgatcccg agttcgatca cgccctcgac ctcgccatcg gcgccgcgca agggccagag
    14341 cagcacgtcg aggtcgacga tgcggccgcc ggggacggtg cgctgcgtcg cgtagtgcgc
    14401 aggctcgccc tgcagcgcgc ggcgcaggtg cggctcgatt tcggcgaatc gttcgtcgcc
    14461 gacgatttcc cgcacctggc ggccgtcgat tccgacgccc ggcaggccat gcagctcggc
    14521 gtacgccttg ttgtgcacga ccacgcgccg gtcggggctc acgagcacga tggcggccgg
    14581 catgaactcg acgagctggc ggaactgcga ttcgctcttg cgcaggcgct gctcggcctg
    14641 cttgcggtcg gtgatgatcc tcgcgagccc gcggtaaccg cggaaccggc cgcgctcatc
    14701 cacgaccggc accgcgccga ccaggagcca ggattcgcgg ccgtcgggcg acacctggcg
    14761 gatctcgagg tcgcggaatg cccgccgttc ctcgagcacc gcgcgatgcg acccccagtc
    14821 ggcctcggtg agttccggcg catccagcgc gggccgcggc agcatcgagt cgagtccgcg
    14881 cgcccatgcc ggggcggacc aggagacgag gttgaagtgc tcgtcctgct cccagtaaag
    14941 gtccgacgac agctcggaca atgcccggaa ccgcgcctcg ctttcggcga gcgatttttc
    15001 ggtgagcttg cgcggcgtga catcggcgtt cgtgccgctc acgtgcaccg cgcggccctt
    15061 cgcgtcgcgg gcaatcacct tgccgcgcgt gcggatccag cgccacgacc ggtcggcgca
    15121 tttcacgcgg aattccgcgt cgagctgcgc gtcgattccc ttgaaggccg caatcgcggt
    15181 cttctgcacc cgctcgaggt cgtcggggtg gacgagcgcg gcgagatcgg caatcgtgca
    15241 gcgcgtctcc ccggcgcttt cgccgagcat ttccttccag ttcgcattga gccagacgga
    15301 atcggcggcg aggtcccagt cccagatggt gagcgacgat cccgcaaggg cattgcgcac
    15361 gcggtcgcgt tccgcatcga ggtcggccat ggtggcgaga tgggcggcac gccggcgccg
    15421 cagcatcacc agcgccacgc ccatcaggaa cgcggcgacg ggcaggatcg cgagcaggaa
    15481 ggcgtcgacg aaggaaaccg gaaagtcgtt cacgcgcgcc tagggttcgc cggggccggc
    15541 ctgcggaatc gctgcgcgca ggcggggaac gagggcatcg acggctgcgc gatcatggcg
    15601 ctcggctgcc ttttcgagct ccgcgccgat ccggccggcc tcggggaatc cgaacggggc
    15661 cgacgagccg cggaaggcgt ggccgacggc gcggatggtg gcgtaatcgt cgtcggcgag
    15721 cgccgcgtcg aggcgggcca ggtccgcgtg gcgctccctg aggaacaggc cggcgagctc
    15781 gcgcacctcg tcggaaatgt cgagcggaac cttgtcgggc ttcggatcgg tcaacgtcgt
    15841 cgcgcctcgg gtccggtcag cccgtgtcgg ccgacttgag ctgcgggaag aggatcacgt
    15901 cgcggatcga cgggctgtcg gtgaagagca tggccagccg gtcgatgccg atgccttccc
    15961 ccgccgtcgg cggcaggccg tactcgagcg cgcggatgta gtcggcgtcg tagtacatcg
    16021 cctcttcgtc gccggcctcc tttgccgccg cctgcccggc gaagcgggcg gcctggtcct
    16081 cgggatcgtt cagctcggaa aagccgttgg ccatctcgcg cccggtgatg aacaactcga
    16141 agcggtcggt cacggccggg ttggcgtcgt tggcgcgggc cagcggcgag acgtccgtcg
    16201 gatgcgccac gatgaaggtg ggctggacga gcctcgcttc ggtcgtcgcc tcgaagagct
    16261 tcagttgcag gagccccagc ccgtccgtcg ggaacacctc gacctcgaag ggggccaggg
    16321 ccaccttgag gtactccgca cgggagagct cccccagcgg gtattgcggg ttgtacttgg
    16381 cgatcgcctc ggccatggtc aggcggtcga agggtttcgc gaggtcgatg gtctcgccct
    16441 ggtaggcgag ggtggtcgtg ccgacgacct tctgcgcgca ttcgcgaagc agcccctcgg
    16501 tgaggtccat gaggtagtgg tagtcccggt acgcctcgta gaactcgatc atcgtgaact
    16561 cgggattgtg gcgcgtgctg atcccctcgt tgcggaagtt gcggttgatc tcgaagacct
    16621 tctccagccc gccgaccacc agcttcttga ggtacagctc gggcgcgatg cgcaggaaca
    16681 tgtccatgtc gagcgcgttg tggtgcgtcg tgaagggctt g
//
LOCUS       DASUEV010000025         5533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1037850, whole genome shotgun sequence.
ACCESSION   DASUEV010000025 DASUEV010000000
VERSION     DASUEV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037850"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFV55_00760"
     CDS             complement(<1..728)
                     /locus_tag="VFV55_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX4942849.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVAIHSEADADAKYVML
                     ADESVCIGPPPPRDSYLNIPAIISAAEVTDAQAIHPGYGFLSENADFAERVENSGFVF
                     IGPTAASIRTMGDKVAAKAAMKRVGVPCVPGSDGALPDDPKEIRRLAALVGYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVNTTRTEAQNAFGNPMVYLEKFLENPRHIEIQVLADE
                     HRSAIYLGERDCSMQRRHQKIIEEA"
     gene            complement(746..1189)
                     /gene="accB"
                     /locus_tag="VFV55_00765"
     CDS             complement(746..1189)
                     /gene="accB"
                     /locus_tag="VFV55_00765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX4942850.1"
                     /translation="MDLRKLKKLIDLVQESGIGELEITEGEERVRISRTSAHAPVVMA
                     SPAPLAAIAIPGDAVVAAPEAPAQPTGHTLKSPMVGTFYRSPSPGTPSFVEVGQVVAK
                     GQTLCIIEAMKLLNEIESDMAGTVKAILVENGQPVEYGQPLFVIE"
     gene            complement(1218..1667)
                     /gene="aroQ"
                     /locus_tag="VFV55_00770"
     CDS             complement(1218..1667)
                     /gene="aroQ"
                     /locus_tag="VFV55_00770"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX4942851.1"
                     /translation="MSAILVLHGPNLNLLGTREPDVYGRVTLADIDRRLVERGRVAGV
                     VVRTFQNNAEAPLIERIHAAKSDETAFIIINPAAFTHTSVALRDALAAVAIPFIEVHL
                     SNVHAREPFRKHSYFTDLAVGVISGLGAQGYELALEAALARLAPAAG"
     gene            complement(1854..2405)
                     /locus_tag="VFV55_00775"
     CDS             complement(1854..2405)
                     /locus_tag="VFV55_00775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX4942852.1"
                     /translation="MRRWLPLAGIGLLAVLAGVSLWLAGRDAAPRPLEPGPIDASPAA
                     IQVARFSDGDGQPRTLAQFSGKVVVVNFWATWCTPCREEMPGFVKLQSRWQGRGVQFV
                     GLAQDDAAKVSAFGRELHINYPLWLGEAEVMDLSRRLGNRTGVLPHTVLLDGKGQVVE
                     SHIGIFAESDLDSRLSDIVGKKH"
     gene            complement(2402..2923)
                     /locus_tag="VFV55_00780"
     CDS             complement(2402..2923)
                     /locus_tag="VFV55_00780"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HEX4942853.1"
                     /translation="MKHLIAIFLMACMAGAAGAAPRAADEPLEPDEAFRPDARLVAGD
                     PSAAGGYERHGIDIEYRIAPGYYLYRNRLHFEISPSTLLIGPPEIPPGIEVDDPFLGR
                     SAIFRERVTIHLPFVVSVAKSGHYKVRITAQGCAEDRICYSPFPQEVVVNIPPGYRVM
                     DTPARPAPRGLPR"
     gene            complement(2920..3261)
                     /gene="cutA"
                     /locus_tag="VFV55_00785"
     CDS             complement(2920..3261)
                     /gene="cutA"
                     /locus_tag="VFV55_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX4942854.1"
                     /translation="MAVIAVLTNLPDSESAFNLARTLVRRRLAACVNVLGPATSFYRW
                     DGREEEARETPVLIKSTRECYPELERVILELHPYTLPEIIAFPAELGLENYLGWVRDE
                     CKPDPEPGATP"
     gene            3310..3696
                     /locus_tag="VFV55_00790"
     CDS             3310..3696
                     /locus_tag="VFV55_00790"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX4942855.1"
                     /translation="MRLLLLAILLGFPVLEIVVLVRLFETYGWGVLVWIVVAAVAGVA
                     LLKEARFALVARVGAALAAGQFSIAAFVDSARTVLAGLLLIFPGIISDFIALVLLLLP
                     RGIPEPVHAHAHADRRVIDGQFRRES"
     gene            3785..4837
                     /locus_tag="VFV55_00795"
     CDS             3785..4837
                     /locus_tag="VFV55_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEX4942856.1"
                     /translation="MPQSPAPAERQVRLEPSGRTFIVNSGETILEAALRQGVGLPYGC
                     RNGACGACKGTLRSGTLAYGEHQEHAITNAEIAAGKALTCCTKPQSDVVLEIRELAGL
                     GDLAIRTLPARVEKIDMPAEDVAILYLKLPAAERLQFLAGQYIDVLMKGGIRRSYSMA
                     NAPHDDTHLQLHVRRTPGGAFSRYVFEEMKERAIIRFEGPLGSFYLREDSDKPMILVA
                     GGTGFAPVKAVIEHAFHKGIDRQMVLYWGARSLADLYLPELPVKWQEEHGNFTFIPVL
                     SDPKPEDDWLGRTGFVHQAVLDDFPSLAGYQVYACGAPAMTDIAKKTFLEERGLPEGE
                     FYCDAFTYSAPVVAVT"
     gene            complement(4861..>5533)
                     /locus_tag="VFV55_00800"
     CDS             complement(4861..>5533)
                     /locus_tag="VFV55_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4942857.1"
                     /translation="GLVTAFLMFYVVPRFSRIYEDIGGNLPFLSQMLMKWGQLLSNHA
                     TALFLSLAALAVIVVRVGSRPQVRERLMRAAWRAPRIGEALQVYHLARFYRTMGMLLR
                     SGMPLVGALGMARGLLAPAMRPSLDAASRSISEGIPASTALTECGLTTPVGLSMLRVG
                     ERTGELGAMMDRVAAFHEDDLSRWVDVATRLFEPLLMAAIGVVIGLIVVLLYLPVFEL
                     ANSLQ"
BASE COUNT          891 a         1794 c         1900 g          948 t
ORIGIN      
        1 gcctcctcga tgatcttctg gtggcgccgc tgcatcgagc agtcgcgctc gcccaggtag
       61 attgcggagc ggtgctcgtc ggccagcacc tggatctcga tgtggcgcgg attctcgagg
      121 aacttctcga ggtagaccat gggattgccg aaggcgttct gcgcttcggt gcgcgtggtg
      181 ttcaccgcgt tcacgagcgc cgcctcggtg tgcacgacgc gcatgccgcg cccgccaccg
      241 ccgccggccg ccttgatgat cacgggatag cccacgagcg ccgccaggcg ccggatctcc
      301 ttggggtcgt cgggcagcgc accgtcggag cccggcacgc aggggacgcc cacgcgcttc
      361 atcgctgcct tggccgccac cttgtcgccc atggtgcgga tggaggcggc cgtcggcccg
      421 atgaagacga agccggagtt ctccacccgc tcggcgaagt ccgcgttttc cgagaggaag
      481 ccgtagccgg gatggatggc ctgtgcgtcc gtcacctccg ccgcgctgat gatggccggg
      541 atgttgaggt agctgtcgcg cggcggcggc gggccgatgc acaccgactc gtcggcgagc
      601 atcacgtact tcgcgtccgc gtcggcctcg gagtggatgg cgaccgtctt gatgcccatc
      661 tcgcggcagg cccgctggat gcggagcgcg atctcgccgc ggttggcgat gaggatcttc
      721 tcgaacatct tggggcgtag cggcactact cgatcacgaa gagcggctgg ccgtactcga
      781 cgggctggcc gttctcgacg aggatcgcct tgaccgtgcc ggccatgtcg gattcgatct
      841 cgttcaggag cttcatcgcc tcgatgatgc agagcgtctg gcccttggcg accacctggc
      901 ccacctcgac gaacgagggc gtgccgggcg agggagagcg atagaaagtg ccgaccatcg
      961 gcgacttgag ggtgtggccc gtgggttggg cgggtgcttc gggcgccgcc acgacggcgt
     1021 cgccggggat cgcgatggcg gccaacggcg cgggagaggc cattaccacg ggcgcgtggg
     1081 cgctcgtgcg gctgatgcgc acccgctcct ccccttccgt gatctccagt tccccgatgc
     1141 ccgactcctg caccaggtcg atcagcttct tcagctttct caggtccatg tcggttctcc
     1201 ggggccccgg gagctcctca gcccgccgca ggggcgaggc gggccagggc ggcctcgaga
     1261 gccagttcgt aaccttgcgc gcccaggccg gagatcacgc cgaccgccag atcggtgaaa
     1321 tacgagtgct tccggaaggg ttcgcgggcg tggacattgg agaggtggac ctcgatgaag
     1381 gggatggcca cggccgccag ggcgtcgcgc aaggcaacgc tggtatgggt gaacgctgca
     1441 ggattgatga tgatgaatgc cgtttcgtcg cttttcgccg catgaatccg ctcgatgagc
     1501 ggggcttcgg cgttgttctg gaatgtccgg acgacgactc cggccacccg cccgcgctcg
     1561 acgagccggc ggtcgatgtc ggccaaggtc acgcgaccat agacatcagg ctcgcgggtt
     1621 cccagcaggt tcaggttggg tccgtggaga acgaggatgg cggacatgac cggcgagttt
     1681 gccggaagtc ggcgcaagat gtccagcttt cggcgacgtt gacgggtgcg tcggcccgtg
     1741 atgcccggtg cggtcggaat ctcggcacga tttgcgcttc cggatactcg ctccccgagt
     1801 gaggttttcg caatagtcgc cgtacagggc agtttcgacg acgttcccag aagttaatgc
     1861 tttttgccta cgatgtcgct caagcgggaa tcgaggtccg attccgcaaa gataccgata
     1921 tgcgactcga cgacctgtcc cttcccgtcc agcagcacgg tgtgcgggag caccccggtt
     1981 cggttgccca ggcggcggga aaggtccatc acctctgcct cgccgagcca gagcgggtag
     2041 ttgatgtgca gttcgcggcc gaacgcggac accttcgcgg cgtcgtcctg tgccaggccc
     2101 acgaactgca cgccgcggcc ctgccaacgc gactgcagct tcacgaaccc gggcatttcc
     2161 tcgcgacaag gcgtgcacca cgtggcccag aagttgacga ccacgacctt ccccgaaaac
     2221 tgtgccaggg ttcgaggctg gccgtcgcca tcgctgaatc gggccacctg gatcgcggcc
     2281 gggctcgcgt cgatgggacc cggctcgagg ggccgcggtg cggcatctcg gcccgcgagc
     2341 cacagggaaa ctccggccag cacggccagc aggccgattc ctgccagggg aagccagcgc
     2401 ctcaacgcgg cagtcccctc ggcgccgggc gcgccggcgt atccataacg cgatatcccg
     2461 gcgggatgtt caccacgact tcctgcggga acggggagta gcagatccgg tcctccgcgc
     2521 acccctgggc agtgatcctg actttgtaat gtcccgattt ggcgacgctg acgacaaatg
     2581 ggagatggat cgttacacgt tcgcggaaga tggctgatct tccgaggaag gggtcatcga
     2641 cttcgatgcc cggcggaatc tcgggagggc cgatgaggag cgtcgacggg gatatctcga
     2701 aatgcaggcg attgcggtac aggtagtagc cgggtgcgat gcggtattcg atatcgatgc
     2761 cgtggcgctc gtaaccccct gccgccgagg gatctcccgc cacgagccgt gcatccgggc
     2821 ggaaggcttc gtcgggctcg aggggttcgt ccgcggcgcg aggcgcggcg cctgcggcgc
     2881 cggccatgca ggccatcagg aagattgcga tcaagtgctt catggcgtcg ctccgggttc
     2941 gggatcaggc ttgcattcgt ccctcaccca ccccagatag ttctccaggc ccagttccgc
     3001 ggggaacgcg atgatctcgg gaagcgtgta gggatgcagt tcgaggatca cgcgttcgag
     3061 ttccgggtag cattcgcggg tggacttgat gagtaccggg gtttcgcgcg cctcctcctc
     3121 gcggccgtcc cagcggtaga aggaagtggc cgggccgagg acgttgacgc acgccgcgag
     3181 gcggcgacgg acaagtgtcc gcgcgagatt gaaagccgac tcggagtcag gtaggtttgt
     3241 aaggacggct atgacggcca cggaagtgtc accggcgcgc acttcgccgg acagtcgagg
     3301 aaggatcgta tgaggcttct gctgcttgca atcttactgg gtttcccggt gctggaaatc
     3361 gtggtcctcg tgcgcctttt cgagacgtac ggctggggtg ttctcgtgtg gatcgtggtg
     3421 gccgcggtgg caggcgtggc gcttctcaag gaggcgcgtt tcgccctcgt ggcgcgggtg
     3481 ggtgcggcgt tggccgccgg gcagttctcc attgccgcct tcgtcgacag cgcgcggacg
     3541 gtgcttgccg gcttgctcct catctttccc ggcatcattt cggatttcat cgcgctcgtg
     3601 ctcctcctgc tgccgcgcgg gattccggag cccgtccacg cgcacgccca cgcggaccgc
     3661 cgggtgatcg acggccagtt ccgccgcgaa agctagcgct cggcccatcg ggtagaattc
     3721 cgcgagcttt tccggcgggg gcgcctgtgc cgcccgccgc ccaggttaca cagaaagaat
     3781 tcccgtgcct caatcccctg ccccggccga gcgccaggtg cgcctcgagc cgagcggccg
     3841 cactttcatc gtcaattccg gcgagaccat cctcgaggcc gcgctgcggc agggcgtggg
     3901 actgccctac gggtgccgca atggcgcctg cggcgcgtgc aagggcacgc tcaggagcgg
     3961 gacgctcgcc tatggcgagc accaggagca cgccatcacc aatgcggaaa tcgccgccgg
     4021 caaggcgctc acctgctgca cgaagccgca atccgacgta gtcctcgaga tccgcgagtt
     4081 ggccggactc ggggacctcg ccatccgcac gcttcccgca cgggtggaga agatcgacat
     4141 gcccgcggag gacgtggcca tcctctacct gaagctgccg gccgccgagc ggctgcagtt
     4201 cctggccggg caatacatcg acgttctcat gaaaggcggc atccggcgca gctactcgat
     4261 ggccaacgct ccgcatgacg acacgcacct gcagctgcac gtgcgcagga cgcccggcgg
     4321 cgccttctcg aggtacgtgt tcgaggagat gaaggagcgc gcgatcatcc gcttcgaggg
     4381 gcccctggga tcgttctacc tccgcgagga ctccgacaag ccgatgatcc tggtggccgg
     4441 cggcacgggg ttcgcgccgg tcaaggcggt gatcgagcac gccttccaca aggggatcga
     4501 ccgccagatg gtgctctact ggggcgcgcg ttccctggcc gacctgtacc tgcccgagct
     4561 tccggtgaag tggcaggagg agcacggcaa cttcaccttc attcccgtgc tctccgaccc
     4621 gaagccggag gacgactggc tgggtcgcac cggcttcgtc caccaggcgg ttctcgacga
     4681 cttccccagc ctcgccggct accaggtgta cgcgtgcggc gcgccggcga tgaccgacat
     4741 cgcgaagaag acgtttctcg aggagcgcgg gctgccggag ggcgagttct actgcgacgc
     4801 tttcacctac tccgcgccgg ttgtcgcggt gacgtagctg gcatcgccgg tcgcggccgc
     4861 ctactggagg ctgttggcca gctcgaagac cgggaggtag agcaggacga cgatcaggcc
     4921 gatgacgacg ccgatcgccg ccatgagcag cggctcgaag agtcgcgtgg cgacgtccac
     4981 ccatcgcgaa aggtcgtcct cgtggaaggc ggcgacgcga tccatcatcg cgcccagctc
     5041 gcccgttctc tccccgacgc gcagcatgct caggcccacc ggcgtagtca ggccgcactc
     5101 cgtgagcgcg gtagaggccg gaatgccttc cgagatgctt cggctggccg catcgaggga
     5161 cgggcgcatg gcgggagcga gcaggccgcg cgccatgccc agcgcaccca cgagcggcat
     5221 cccgcttctc aggagcattc ccatcgtccg gtagaaccgc gccaggtggt agacctggag
     5281 cgcctctccg atgcgcggtg cgcgccaggc ggcccgcatc aggcgctcgc ggacctgagg
     5341 ccgcgatccc acccggacca cgatcaccgc cagtgcggcc agcgacagga aaagcgccgt
     5401 cgcgtgattg gaaagcagct ggccccactt catgagcatc tgcgacagga acggcaggtt
     5461 gccgccgatg tcctcgtaga tgcggctgaa gcgcggcacg acgtagaaca tcaggaacgc
     5521 agtgacgaga ccg
//
LOCUS       DASUEV010000026         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_347148, whole genome shotgun sequence.
ACCESSION   DASUEV010000026 DASUEV010000000
VERSION     DASUEV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347148"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFV55_00805"
     CDS             complement(<1..268)
                     /locus_tag="VFV55_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4942858.1"
                     /translation="MAVSFRSGSLARNARGKDRRHRGRRDRGGIGDIIVHEYRPMRGP
                     SMDNLTDIAAFVRVVELGSFTRAADDLDLSKAVVSKYLTRLEERL"
     gene            267..650
                     /locus_tag="VFV55_00810"
     CDS             267..650
                     /locus_tag="VFV55_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4942859.1"
                     /translation="MDYDFTSAARHFAAKNAFTTGPHELAGMIGAKADIVIVDVRYPA
                     DFRASRIPGAINLPKGKWEKPAGLSKEKLNVLYCYNPQCHLASEAASALLALGYPVAE
                     MEGGFSTWEAFGSPVETPAATAVAA"
     gene            664..1113
                     /locus_tag="VFV55_00815"
     CDS             664..1113
                     /locus_tag="VFV55_00815"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX4942860.1"
                     /translation="MIDRTTAPLGALLLRVSLGAMFIAHSVYLKAFVFTLPGTAKFFA
                     SLGLPGPLAYVVFAMEAIGGALLILGVKTRWVSIALVPVLAGAAWSHAGNGWLFTNAG
                     GGWEYPVFLAIAAVVQSLVGDGAYALSKGATPAGISPELSRAGRYAL"
     gene            complement(1198..1620)
                     /locus_tag="VFV55_00820"
     CDS             complement(1198..1620)
                     /locus_tag="VFV55_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX4942861.1"
                     /translation="MSRLLVALQFGLIALVALRVQPARIGPAFMVLMAAGVAVGGWAL
                     AANRPGNFFIRPEPRPGGRLVTHGPYAFVRHPMYLAVLLATAAFAVAGDEWQWVAWFA
                     LAGVLAAKARREERGLVLQHPGYEAYRAKTRAIVPYLF"
     gene            complement(1628..2080)
                     /locus_tag="VFV55_00825"
     CDS             complement(1628..2080)
                     /locus_tag="VFV55_00825"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEX4942862.1"
                     /translation="MWLDALLAYLHFTAIFVLFAFLTVEVMLARGELDAKAVRMLGRV
                     DLWYVGAAIAALATGLARANWGAKGWAFYAGDWVFWAKVASIAVVALLSIPPSVAFIR
                     WRRRAEAQPGLVVPEDERKRMRRYLMWEVHVVALIPLFAVVMSRGLAR"
     gene            complement(2083..3267)
                     /locus_tag="VFV55_00830"
     CDS             complement(2083..3267)
                     /locus_tag="VFV55_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX4942863.1"
                     /translation="MTGRAGKLDLAARMGHIAPFEVMEIQTVARRLEAEGRDVVHLEI
                     GEPDFATPAPIVEAAKRALDEKPMFYTSALGITPLREAISRFYRERYGVEVAAERIVV
                     TAGSSAALLLAFGVLLDPGDEVLLADPSYPCNRHFVHALGGLPRLVPVDSRTRYQLTP
                     ELAREAWTPRTRIAMVASPSNPTGTMVEPGEVAALAALARERGGTLLADEIYHGLTYG
                     HDARTALEAGDDVFVINSFSKYFQMTGWRLGWLVAPVAYVREIEKLAQNVYISASTLA
                     QHAALAAFAPGTIAILESRRRELAARRDYLLPALESLGFRIAVKPEGAFYIYADCSRL
                     AQDSFVFAREVLEKAGVAITPGKDFGFHHPERHIRIAYTQPIARLEQAVARLGDFLAG
                     RA"
     gene            complement(3278..3706)
                     /locus_tag="VFV55_00835"
     CDS             complement(3278..3706)
                     /locus_tag="VFV55_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX4942864.1"
                     /translation="MSDTEQVLWQGNPSQWTNFWPFVACLLVIPIPWAIWRWLSTRCY
                     FYEVTGERIRIRQGVFSRRTDSLELFRVKDASFVQPFVMRLVGIGNVEIETSDSTTPL
                     LVIRGVPDADQLRERILKAVDTVRDRKGVREVDFSGRPQG"
     gene            complement(3758..>4053)
                     /locus_tag="VFV55_00840"
     CDS             complement(3758..>4053)
                     /locus_tag="VFV55_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX4942865.1"
                     /translation="TPWERLEHIPRFLKGGALRLQKYRANAERDLRHAPVVAGLWAQY
                     EADREAGKGGARLEEFRWLVEELRISLFAQELKTPFPVSAKRLQKFRDEYLRR"
BASE COUNT          673 a         1419 c         1361 g          600 t
ORIGIN      
        1 ccagccgttc ctccagccgc gtgaggtact tgctgaccac cgccttcgag aggtcgagat
       61 cgtccgcggc gcgggtgaag ctgccgagtt ccaccacgcg cacgaaggct gcgatgtcgg
      121 tgaggttatc catggagggg ccccgcattg gtcgatattc gtgaacaatg atatcgccaa
      181 tgccccctcg atcgcggcgg ccgcgatgtc tacgatcctt tccacgcgca ttccgcgcca
      241 atgaaccgga tcgaaaggac accgccatgg actacgactt caccagcgcc gcaaggcact
      301 tcgccgcgaa gaacgccttc accaccggcc cgcacgagct ggccggcatg atcggcgcga
      361 aggccgacat cgtcatcgtc gacgttcgct accctgccga cttccgcgcc tcgcgcatcc
      421 cgggcgccat caacctgccc aagggcaagt gggaaaagcc ggccgggctc tcgaaggaga
      481 agctgaacgt cctctattgc tacaacccgc agtgccacct cgcctccgaa gccgcgtcgg
      541 ccctgctggc gctgggctat ccggtcgccg agatggaagg cggtttttcc acgtgggaag
      601 ccttcggcag ccccgtcgag accccggcgg cgaccgcggt cgccgcatga gcgggaggag
      661 accatgatcg accggaccac ggcgcccctc ggcgcgctcc tgctgcgcgt ctcgctcggt
      721 gcgatgttca tcgcgcacag cgtttacctg aaggccttcg tcttcaccct gcccggcacc
      781 gcgaagttct tcgcctcgct gggactgccc ggccccctcg cctatgtcgt cttcgcgatg
      841 gaggccatcg gaggcgctct cctcatcctc ggcgtgaaga cgcgctgggt gtcgatcgcc
      901 ctcgtgccgg tccttgcggg cgccgcctgg tcgcacgccg gcaacggctg gctgttcacg
      961 aacgcgggcg gcgggtggga gtacccggtg ttcctcgcca tcgctgccgt cgtgcagtcc
     1021 ctcgtgggcg atggtgccta tgcgctgtcg aagggtgcca ctcccgctgg catcagcccg
     1081 gagctctcgc gtgccgggcg ctacgcgctt tgatgcgcac ggtcgagccg catgcggaaa
     1141 gcctctgccg ccggcggctc ggcctgatcc ggagcgtcgc gaccatgtga tccccggtca
     1201 gaaaaggtaa gggacgatcg cgcgagtttt cgcgcgatac gcctcatagc cgggatgctg
     1261 gagcaccagc ccgcgctcct cgcgccgcgc cttcgcggcg agcactcccg cgagcgcgaa
     1321 ccaggccacc cactgccact cgtccccggc aacggcgaac gcggcggtcg ccaggagcac
     1381 ggcgaggtac atcggatggc gcacgaacgc atagggccca tgggtcacga gccggccgcc
     1441 gggacgaggc tcgggccgga tgaagaagtt gccgggccgg ttggccgcga gcgcccagcc
     1501 acccacggca acgccggcgg ccatcaagac catgaacgcg gggccgatcc gggcgggctg
     1561 gacgcgaagc gcgaccagcg cgatcagtcc gaactggagc gcgacgagca accttgacac
     1621 ggcaacctca gcgcgcgagc ccgcgcgaca tcaccaccgc gaaaagcggg atgagagcga
     1681 ccacgtgcac ttcccacatg aggtagcggc gcatgcgctt tcgctcgtcc tcgggaacca
     1741 cgagcccggg ctgcgcctcg gcgcgccggc gccagcggat gaacgcgacc gaaggcggga
     1801 tcgagagcag ggccaccacg gcgatcgacg ccaccttggc ccagaacacc cagtcccccg
     1861 catagaacgc ccagcccttg gcgccccaat tcgcgcgtgc gagccccgtc gccagggccg
     1921 cgatggcggc ccccacgtac cacaggtcca cgcggccgag catccggacg gccttcgcgt
     1981 ccagctcgcc gcgggccagc atcacctcga cggtgaggaa cgcgaagagc acgaatatcg
     2041 ccgtgaagtg gaggtaggcg agcagtgcgt cgagccacat ggtcaggccc tccccgcgag
     2101 gaaatcgccc aggcgcgcca cggcctgctc gagccgcgcg atgggctggg tgtaggcgat
     2161 gcggatgtgc ctttccggat ggtggaaacc gaagtccttg ccgggggtga tcgcgactcc
     2221 cgccttctcg aggacttcgc gcgcgaaaac gaagctgtcc tgcgccaggc gcgagcagtc
     2281 cgcgtagatg tagaaggccc cctccggctt caccgcgatg cgaaagccga gcgactcgag
     2341 ggccggcagg aggtagtcgc ggcgcgcggc aagctcgcgg cggcgcgatt cgaggatcgc
     2401 gatcgtcccc ggcgcgaatg cggccagcgc cgcgtgctgc gcgagcgtgg aggccgagat
     2461 gtacacgttc tgcgcaagct tctcgatctc gcgcacgtac gccaccggcg ccacgagcca
     2521 gcccaggcgc cagccggtca tctggaaata cttcgagaag ctgttgatga cgaagacgtc
     2581 gtccccggcc tccagcgcgg tgcgcgcgtc gtggccatag gtgaggccgt ggtagatctc
     2641 gtctgcgagg agcgttccgc cgcgctcgcg cgcgagcgcc gccagcgcgg cgacctcgcc
     2701 cggttccacc atcgtgcccg tgggattgga agggctcgcc accatggcga tgcgcgtgcg
     2761 cggcgtccac gcttcccgcg caagctccgg cgtgagctgg tagcgcgtgc gcgaatcgac
     2821 ggggacaagc ctcgggagcc cgcccagggc gtgcacgaaa tggcggttgc acgggtagct
     2881 cggatcggcg agcaggacct cgtcgcccgg atcgagcagc acgccgaaag ccagcagcaa
     2941 tgccgcgctc gaccccgcgg tgaccacgat gcgctccgcg gccacctcca cgccataccg
     3001 ctcccggtag aagcgcgaga tcgcctcgcg aagcggcgtg atgccgaggg cggaggtata
     3061 gaacatgggc ttctcgtcca gcgcgcgctt ggccgcctcg acgatcggtg ccggtgtcgc
     3121 gaaatcgggc tcgccgatct cgaggtggac cacgtcgcgg ccttcggcct ccagccgtcg
     3181 cgcgacggtc tggatctcca tcacctcgaa gggagcgatg tggcccatgc gggccgccag
     3241 atcgagtttg ccagcgcggc cggtcatgac cagtccctca gccctgcggg cggccgctga
     3301 aatcgacctc gcgaaccccc ttgcggtcgc gcacggtgtc cacggccttg aggatgcgct
     3361 cgcgaagctg gtcggcgtcg ggcacgccgc ggatcacgag caaaggcgtc gtcgagtcgg
     3421 aggtctcgat ctccacgttg ccgatgccca cgaggcgcat cacgaacggc tgcacgaagc
     3481 tcgcgtcctt cacgcggaac agctcgaggc tgtcggtgcg ccggctgaac acgccctggc
     3541 ggatgcggat tcgctcgcct gtcacttcat agaagtagca gcgcgtggaa agccagcgcc
     3601 agatcgccca cggaatgggg atcacgagga ggcacgccac gaagggccag aagttggtcc
     3661 actgcgaggg gttgccctgc cacaggacct gctcggtatc gctcatggtc ggatcgtcat
     3721 cagggaaatg ttcgggactg taccgcaaat gcgccgctca acgccgcagg tactcgtcgc
     3781 ggaatttctg caggcgcttg gccgaaaccg gaaacggcgt cttgagctcc tgcgcgaaca
     3841 gcgagatgcg cagctcctcc accagccagc ggaattcctc gagcctcgcc ccgcccttgc
     3901 ccgcctcgcg gtcggcctcg tactgtgccc acagcccggc gacgacgggg gcgtggcgca
     3961 ggtcgcgctc ggcgttggca cggtacttct gcagcctgag cgcgccgccc ttgaggaagc
     4021 gcggaatgtg ctcgagcctc tcccacggcg tgc
//
LOCUS       DASUEV010000027         6322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_580812, whole genome shotgun sequence.
ACCESSION   DASUEV010000027 DASUEV010000000
VERSION     DASUEV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580812"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..954)
                     /locus_tag="VFV55_00845"
     CDS             complement(199..954)
                     /locus_tag="VFV55_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4942866.1"
                     /translation="MNELAQLPVVFWIVAPLTILFAYTMFGMSGFGSTVIALPILANW
                     LPLTYLVPLMALGDLVAAVVVGGSNRRHVSRPELKRLLPFMVAGIALGVTVLVNVPQQ
                     PLKIGLAAFAMAVGLYSILNPSPKGTISPWWCIPAGTVAGILAAVFGAGGPVNVAYLA
                     GRLHDKGEMRSTISVIISVSATLRTVLYAMAGLVLKAGTLAGFALAAPFAWAGLVLGS
                     RIHTGLTQAQMRRTIGGLLVASGGILLARTLLG"
     gene            complement(951..1862)
                     /gene="xerD"
                     /locus_tag="VFV55_00850"
     CDS             complement(951..1862)
                     /gene="xerD"
                     /locus_tag="VFV55_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX4942867.1"
                     /translation="MKAPAEADLRLVERFCDALWLADGLARNTLEAYRRDVTQFAAWL
                     ATDPGKGLLAAGPEHLHRHLAWQVTSKKARPRSTGRLVSALKRFYRYALLEGLRRDDP
                     SADIEAPKLPRSLPRSLSEAEVESLLAAPDTESDLGLRDKAMLETLYASGLRVSELIG
                     LKVQQVSLDMGVVRVLGKGSKERLAPLGEEALDWIQRYQKLARPLILGARKSDALFVT
                     ARGGAMTRQAFWGLVKRYAARAAIRSPISPHTLRHAFATHLINHGADLRVVQMLLGHA
                     DISTTQIYTHVARERLKRIHAKHHPRG"
     gene            complement(1840..2322)
                     /locus_tag="VFV55_00855"
     CDS             complement(1840..2322)
                     /locus_tag="VFV55_00855"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4942868.1"
                     /translation="MAPFQACLRAPFGTLGVSATDTHVTGIRYLSPDVPALAPKRNSI
                     AFLACVQLQSYLDDPAFAFDLPLKLAGTRHQLAVWEAMQAIPPGETRTYGELAKAIGS
                     SARAVGAACGANPIPIVVPCHRVVGAGGALGGFMGAREEGFELSIKRWLLAHEGARRG
                     "
     gene            complement(2328..3338)
                     /locus_tag="VFV55_00860"
     CDS             complement(2328..3338)
                     /locus_tag="VFV55_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4942869.1"
                     /translation="MTERFAPAPLQDLMRRALEKAGATPAMAAATAAALVAAELDGIA
                     SHGASRIPQYCGHVKNGRATGSAVPKVVRERGGACLVDAGGGLAFEACALAVREVIAR
                     ARASGVAFASVANSNHFGVAAYHVEPIAQAGLVGLAFGNSPAAMPMWGGKRPLFGTNP
                     IAAAFPRRSAAPLVVDMSLSEVARGKLMVAAREGKTIPHGWALDKDGNPTTDPEAGLG
                     GMMLPAGGVKGAMLALTVELLVCALSGAAFGFESDSFFTDAGKPTRIGQAFLAIDPDA
                     LAGREVFLERIETLVAAMLSDEGVRLPGDRRRANRERIAREGIAIAPDLAAKIRALAG
                     EA"
     gene            complement(3335..5317)
                     /locus_tag="VFV55_00865"
     CDS             complement(3335..5317)
                     /locus_tag="VFV55_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX4942870.1"
                     /translation="MSQPAAPEDDLLPPDGLSEDLRHRVEEFIEEEEGRFNNYKSWVA
                     AFLTAVAVGTSAFHLYAAYGIVRTDLLRGIHVGLVLFLSFMLFPVARRFRHRLMWFDV
                     ALALLSLVVIGWMLLGGDDFTDRNTDPGTWDIIFGVALMLLVVEATRRTSGWIMPIVI
                     SFFLVYAFVGPWLPHPWTHRGYDLGRLVGHMYMTLEGIFGTAVDVSATLIILFTIYGA
                     FLQYSGAGKFFLDFSFALMGGKSSSAGRAVVMASFLLGGPSGSGVATTVTIGSVAYPM
                     LKRSGYEPAAAGGLLAAGGLGAILSPPVLGAAAFLIAEFLKISYLEVIKMAVIPTILY
                     YFCLYFMVELDARKYGMSEGAFETVESAWTLSKRYWYHFLSLASIIVFMLMGFSPTLA
                     VFWATVTAFVVSFVRRETALRPRRFVKAMETGTTGVLNVAATCASAGIIVGVVTLTGL
                     GLKFSSIVIDYAGGSLALTAVYTAAIVWIVGLAVPVTASYIICAVIAAPALITLGVPD
                     FAAHMFIFYYALLSEVSPPTALSCFAAAAITGADPYKTTLQAWKYTMPAFVVPFFFVL
                     DPLGTGVLLQLPKGANWLEVSWVVFLIFVAIAALAAGLQGWLLRKTNIVERALLVLSG
                     VLVIIPVASLDAVGVGMFALVFVLQFLRRSPVPATS"
     gene            complement(5419..>6322)
                     /locus_tag="VFV55_00870"
     CDS             complement(5419..>6322)
                     /locus_tag="VFV55_00870"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX4942871.1"
                     /translation="QWAKKVADGIAATGKPVAWFGIEQHGDHDTIMRASKKAKEFVQW
                     ASERTREERPLKDLWVSTKCGESDTTSGCGANPAVGDAFDKLYPHGTTLCFGETTELT
                     GGEHLVAARCRTDEVRKKFQFMFDRYQAVIEAHKTSDLSDSQPTKGNIEGGLTTIEEK
                     ALGNIQKIGRKCVVDGVIDKAETPTGPGLWFMDSSSAAAEMLTLLGAAGFAVHFFPTG
                     QGNVIGNPILPVIKLSANPRTVRTMGEHIDVDVSALLRKEMNMDQAGDALLACMFRTA
                     NGRLTSAEALGHREFVLTRLYESA"
BASE COUNT         1071 a         2113 c         2222 g          916 t
ORIGIN      
        1 ccacctcgtg ctcgggcacg ccgagcgacg aggacgacac cgccgttccg ccgtgttcga
       61 cgtaatcgta gatgtgctcg gtgtaagcca cgccgtgctg cgcgaggaag atggtagccg
      121 gggttcccgt atgctttccc atggtcaagg tcggtgcagt gaggttggca cgatggtgat
      181 cgtagaggaa gttccggctt atccgagcag cgtgcgggcg agaagaatgc cgccacttgc
      241 cacgagcagg ccgccgatgg tgcgccgcat ctgcgcctgc gtgagtcccg tgtggatccg
      301 cgagccgaga accagtcccg cccacgcgaa cggcgccgcc agcgcgaacc cggccagcgt
      361 cccggccttg aggacgagcc ccgccatggc atagagcacc gtgcgcagcg tcgccgagac
      421 ggaaatgatg accgagatcg tggagcgcat ctcgcccttg tcgtgcagcc ggcccgcaag
      481 ataggcaacg ttgaccggcc ctccggcgcc gaataccgcc gcaaggatgc cggcaacggt
      541 cccggccgga atgcaccacc atggcgagat cgtgcccttg ggcgacgggt tcaggatcga
      601 atacaggccg accgccatcg cgaaggcggc cagcccgatc ttgagcggct gctgcgggac
      661 gttcaccagc acggtgacgc ccagcgcgat gcccgccacc atgaacggca gcagtcgctt
      721 gagttccggc ctgctcacgt ggcggcggtt cgagccgccg acgacgaccg cggccacgag
      781 gtcgcccagc gccatgagcg gcacgaggta ggtgagcgga agccagttgg cgagtatcgg
      841 aagggcgatc acggtggagc cgaaaccgga catgccgaac atggtgtagg cgaacaggat
      901 ggtgagcggc gcgacgatcc agaagacgac gggcagctgc gcgagctcgt tcatccgcgg
      961 gggtggtgct tcgcgtggat ccgcttcagc cgctcgcgcg caacgtgcgt gtagatctgc
     1021 gtggtggaga tgtcggcgtg gcccagcagc atctggacga cgcgcaggtc cgcgccgtgg
     1081 ttgatgaggt gcgtggcgaa ggcatggcgg agagtgtgcg gcgagatggg ggagcggatc
     1141 gcggcgcgcg cggcgtagcg cttgaccagg ccccagaacg cctgccgcgt catcgcgccc
     1201 ccacgggcgg tgacgaaaag cgcgtcgctc ttcctcgccc ccaggatgag cggccgcgcg
     1261 agcttctggt agcgctggat ccagtcgagg gcctcctccc ccagcggcgc gaggcgttcc
     1321 ttggatccct tgccgagcac ccgtaccacg cccatgtcga ggctcacctg ctgcaccttg
     1381 agcccgatca gctcggagac gcgcagcccg ctcgcataga gcgtctcaag catcgccttg
     1441 tcacgcagcc cgaggtccga ttcggtgtcc ggcgcggcga gcagcgactc gacttcggcc
     1501 tcgctgagcg acctggggag cgagcgcggc agcttcggcg cctcgatgtc ggcggaaggg
     1561 tcgtcgcggc ggagcccctc cagcagcgcg taccggtaga agcgcttgag ggccgatacg
     1621 agccggccgg tgctgcgcgg cctggccttc ttcgacgtca cctgccaggc gaggtggcgg
     1681 tgcaggtgct cggggccggc cgccaggagc cccttgccgg gatccgtggc cagccacgcg
     1741 gcgaattgcg tcacgtcccg ccggtaggct tccagcgtat tgcgggcgag gccgtcggcg
     1801 agccagagcg catcgcagaa gcgctcgacg aggcgcaggt cagcctcggc gggcgccttc
     1861 atgcgcaagc agccatcgct tgatcgacag ttcgaagcct tcctcgcgcg cgcccatgaa
     1921 gccgcccagc gcaccgcctg cgccgacgac gcgatggcac ggcaccacga tgggaatcgg
     1981 attggcgccg cacgcggcgc cgaccgcgcg cgccgaggag ccgatcgcct tcgcgagctc
     2041 gccgtaggtg cgcgtctcgc cggggggaat cgcctgcatc gcctcccaca ccgcgagctg
     2101 gtggcgcgtg cccgcgagct tcagcggcag gtcgaaggca aaggcgggat cgtcgagata
     2161 ggactgcaac tgcacgcagg cgaggaaggc aatgctgttc ctcttcggcg cgagcgccgg
     2221 gacatcgggc gacagatagc ggatgccggt cacgtgcgtg tccgtcgccg acacgccgag
     2281 cgtgccgaaa ggcgcccgca agcaggcctg gaacggggcc acggcgctca ggcctccccc
     2341 gccagcgctc ggatcttcgc cgcgaggtcc ggcgcgatcg cgatgccctc cctcgcgatc
     2401 cgctcgcggt tggcgcggcg acggtcgccg ggaaggcgca cgccctcgtc ggaaagcatc
     2461 gccgccacga gggtctcgat gcgctccagg aacacctcgc ggcccgcgag cgcgtccggg
     2521 tcgatggcca ggaaagcctg cccgatgcgg gtgggctttc cggcatcggt gaagaagctg
     2581 tcggactcga atccgaacgc ggcgccggaa agcgcgcaga cgagcagctc cacggtaagc
     2641 gcaagcatcg cccccttgac gccgccggcg ggcagcatca tgcctcccag cccggcctcc
     2701 ggatcggtgg tgggattgcc gtccttgtcc agcgcccagc cgtgcgggat ggtcttgcct
     2761 tcgcgcgcgg ccaccatgag cttgccgcgc gcgacttccg acaggctcat gtcgaccacg
     2821 agcggcgcgg cgctgcggcg cgggaacgcg gcggcgatcg ggttggtgcc gaagaggggc
     2881 cgcttcccgc cccacatcgg catggcggcc ggggaatttc cgaacgccag cccgacgagc
     2941 cccgcctgcg cgatgggctc gacgtggtat gcggccacgc cgaaatggtt gctgttggcc
     3001 accgacgcga aggccacgcc gctggcgcgc gcacgcgcaa tgacctcgcg cacggcaagc
     3061 gcgcacgcct cgaacgcgag cccgccaccc gcgtccacga ggcaggcgcc accgcgttcg
     3121 cgcacgactt tcggcacggc cgatccggtg gcgcggccgt tcttcacgtg cccgcagtac
     3181 tggggaatgc gcgaggcccc gtgggaggca atgccgtcga gttccgcagc cacgagcgcg
     3241 gcggcggtag cggccgccat tgcaggcgtg gcacccgcct tctcgagcgc gcggcgcatg
     3301 agatcctgga gcggcgcagg ggcgaagcgt tcggtcatga ggttgcgggc acgggcgacc
     3361 tgcgcaggaa ctgcaggacg aacacgaggg cgaacatccc gacgccgacg gcatccaggc
     3421 tggccacggg aatgatcacg agcacgccgg acagcaccag gagcgcccgc tcgacgatat
     3481 tggtcttgcg caggagccag ccctgcagcc cggccgcgag cgccgcgatg gccacgaaga
     3541 tgaggaacac gacccagctc acctcgagcc agttggcgcc cttgggcaac tgcagcagta
     3601 ccccggtgcc gagcggatcg agcacgaaga agaacggcac cacgaacgcg ggcatcgtgt
     3661 acttccatgc ctgcaacgtg gtcttgtagg gatccgcgcc ggtgatcgcg gcggcggcga
     3721 agcacgacag cgcagtgggc ggcgacacct cggacaggag cgcgtagtag aagatgaaca
     3781 tgtgcgccgc gaagtccggc acgcccagcg tgatgagcgc gggcgcggcg atcaccgcgc
     3841 agatgatgta ggacgccgtc accggaaccg cgaggcccac gatccagacg atggccgcgg
     3901 tgtagacggc cgtgagtgcg aggctgccgc ccgcgtagtc gatcacgatc gacgagaact
     3961 tgagccccag cccggtgagc gtcaccacgc ccacgatgat gcccgccgag gcgcaggtgg
     4021 ccgccacgtt gagcacgccg gtcgtgcccg tctccatcgc cttcacgaag cgcctgggtc
     4081 gcaatgccgt ctcgcggcgg acgaagctca ccacgaaagc ggtcaccgtg gcccagaaca
     4141 ccgcgagcgt cggcgagaag cccatcagca tgaagacgat gatcgaggcg agcgacagga
     4201 agtgatacca gtagcgcttc gacagcgtcc atgcgctttc caccgtctcg aacgcccctt
     4261 cgctcatgcc gtacttgcgc gcgtcgagtt ccaccatgaa gtacaggcag aagtagtaga
     4321 ggatggtcgg gatgaccgcc atcttgatca cttccaggta ggagatcttg aggaactccg
     4381 cgatgaggaa ggccgccgcg cccagcacag gcggcgagag gatggcgcca aggccgcccg
     4441 cggcgagcag cccccctgcc gcggcgggct cgtagccgct ccgcttgagc atcgggtagg
     4501 ccaccgagcc gatggtgacc gtggtggcca cgcccgagcc ggaaggcccc cccagcagga
     4561 agctcgccat caccaccgcg cgcccggcgc tgctggactt cccgcccatc agcgcgaagg
     4621 agaagtcgag gaagaacttg ccggcgccgg aatactgcag gaacgcgccg tagatggtga
     4681 acaggatgat gagcgtggcg gacacgtcga cggcggtgcc gaagatgccc tcgagcgtca
     4741 tgtacatgtg ccccaccagc cgtcccaggt cgtagccgcg gtgcgtccag ggatgcggca
     4801 gccaggggcc gacgaaggca tagaccagga agaaactgat gacgatgggc atgatccagc
     4861 ccgaggtgcg gcgcgtggcc tcgacgacga gcagcatgag cgccacgccg aagatgatgt
     4921 cccaggtccc ggggtcggtg ttgcggtcgg tgaagtcgtc gccgccgagc agcatccagc
     4981 cgatcacgac gagcgacagt aacgcgagcg ccacgtcgaa ccacatcagc cggtggcgga
     5041 agcgcctggc caccgggaag agcatgaacg acaggaacag cacgaggccc acgtggatgc
     5101 cgcgcaggag gtccgtgcgc acgatcccgt aggccgcata gaggtggaac gcggacgtgc
     5161 cgacggccac ggccgtcagg aaggcggcga cccagctctt gtagttgttg aaccggcctt
     5221 cctcctcctc gatgaactcc tcgaccctgt ggcgcaggtc ttcgctgagc ccatcggggg
     5281 gcaggagatc gtcctcgggt gctgcgggct gactcatggc tcctccaggg cgccctgtgg
     5341 ccgggcgctg ccgccattgt ccgcaaaaaa tcggggacag tccccatttt tcagggactg
     5401 tccccgattt attctgcgct aggcgctctc gtagagacgg gtgagcacga actcgcggtg
     5461 gccgagggcc tcggcggagg tgaggcggcc gttggcggtg cggaacatgc acgcgaggag
     5521 cgcgtcgccc gcctggtcca tgttcatttc cttgcgcagc agggcggaga cgtccacgtc
     5581 gatgtgctcg cccatcgtgc gcacggtgcg cggattggcg gagagcttga tcaccggcag
     5641 gatggggttg ccgatcacgt ttccctgccc cgtggggaag aaatggaccg cgaagcccgc
     5701 ggcccccagg agcgtgagca tttccgccgc cgccgacgac gagtccatga accacaggcc
     5761 ggggccggtg ggcgtctccg ccttgtcgat gacgccgtcc acgacgcact tgcggccgat
     5821 tttctggatg ttgcccagcg ccttttcctc gatcgtcgtg aggccgccct cgatgttgcc
     5881 cttggtgggc tgggaatccg aaaggtcgct cgtcttgtgc gcctcgatca ccgcctggta
     5941 gcggtcgaac atgaactgga acttcttgcg cacctcgtcg gtgcggcagc gcgcggccac
     6001 gaggtgctcg ccgccggtga gctcggtcgt ctccccgaag cagagcgtcg tgccgtgcgg
     6061 atagagcttg tcgaaggcgt ctcccactgc gggattcgcg ccgcagcccg aggtcgtgtc
     6121 ggattccccg cacttcgtgg agacccacag gtccttgagc gggcgctcct cgcgcgtgcg
     6181 ctcgctggcc cactgcacga actccttcgc cttcttcgag gcgcgcatga tcgtgtcgtg
     6241 gtcgccgtgc tgctcgatgc cgaaccaggc gacgggcttg ccggtggcgg cgatgccgtc
     6301 ggccaccttc ttcgcccact gc
//
LOCUS       DASUEV010000028         6760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_466288, whole genome shotgun sequence.
ACCESSION   DASUEV010000028 DASUEV010000000
VERSION     DASUEV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466288"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFV55_00875"
     rRNA            <1..205
                     /locus_tag="VFV55_00875"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            320..433
                     /gene="rrf"
                     /locus_tag="VFV55_00880"
     rRNA            320..433
                     /gene="rrf"
                     /locus_tag="VFV55_00880"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            549..1673
                     /locus_tag="VFV55_00885"
     CDS             549..1673
                     /locus_tag="VFV55_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX4942872.1"
                     /translation="MKARAAVLHTVGAPPPYAQSRPLRIEEIEIAPPGPGEVLVRVHA
                     AGLCHSDLSVINGDRPRPVPMALGHEAAGEVVECGPLVADLQPGDHVVMVFVPSCGHC
                     VPCMEGRPALCEPGAASNTAGMLLGGGSRLSMAGKPVYHHMGVSAFADHAVVSRRSLV
                     KVDPTLAFDEAALFGCAVLTGVGAVVNTAKMTPGTTAAVVGLGGVGMNSILAASLSGA
                     RVLVAVDLFDDKLELARSLGATHAVNARAPDAVEQVREASGGGVDFAFEMAGSVQAME
                     LAYRVTRRGGTTVTAGLPPPDHRWPLQHVNLTAEERTVKGSYIGSCVPVRDIPRYIAL
                     YKGGRLPVDKLMSDHLKLEDINIGFDRLASGRVVRQVVML"
     gene            1701..3584
                     /locus_tag="VFV55_00890"
     CDS             1701..3584
                     /locus_tag="VFV55_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4942873.1"
                     /translation="MATSSAPLPEKATQVRSLAAFARRLWTRPRSRSAALYLFVVLLL
                     AVASRVPDGDDAPLARLERTAFDWQMQMLREYWPRPIANDVVLVGIDEGSEEIFTEPV
                     ALWHRHFARLLVALADARPRAIGIDVVPPERSYDDIVPGYDLALFRAIRDARQKTATV
                     FALTVDREGRAARMHPTFARALGEEGLGIDQQLRDPDSVSRRFSEADLGGPQAARTLV
                     GQMLRAMGMPVDAGYIDYSVGSRVEYIPMQDVIAWKEQGDGARIEKALGGRIVLVGYV
                     MKRADRWELPVRLVDIDKADGRARFSQPGVITHLQVLRSHLASGLLKPMTQELRWALC
                     LLAAGLAFLRVRQTIAFAVVLALTAVICAAGLFAIRANQVLLPVASIVFTLWAGFTTR
                     AIADGIENTLERGRLRRTFAGQVSPAVMNEMLAGSLSPGVSGQLAEVCILFSDIRDFT
                     ALSERMPPVVVTTVLQRYFDRMVQAVHRHEGTVDKFIGDGMMVLFGAPRRSADPCGDA
                     VKCSLAMMSGLDDLNAEFLSEGLPTLTVGIGINFGVVTVGNIGSSERHNYSAIGDAVN
                     VAARLEGLTKELGRKILISDAVVGRMGQGFQFDPLGSHKVKGHSPVNVWGIRTARPAP
                     AAK"
     gene            3596..4435
                     /locus_tag="VFV55_00895"
     CDS             3596..4435
                     /locus_tag="VFV55_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942874.1"
                     /translation="MKKVLRALFALAATLAVPCAHAASGIAFITDLKGEVAVDGTPRP
                     LLLSELARGQRIIVGTEAQLAVMFIQSGKEYVLKGPADYVVGEREITATHGMPPAARE
                     TPWRASNQVLVKVAQTSGASIRMRSLAPVKSDTKPRLDFPTRGAVATLQPTLRWSNLP
                     DGKVEVAIAVAGKEDKPLAKAKVSGGSHRFPVKLRPDTEYAWTVSFEGREIGSATFRT
                     LPAAALQNAEQRRPSEKAEFSDRLLYALLLQEIGATQEAQEAWGKLAEERTDLPELAG
                     LAK"
     gene            4464..5291
                     /locus_tag="VFV55_00900"
     CDS             4464..5291
                     /locus_tag="VFV55_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942875.1"
                     /translation="MAHLTRSFLVGFLLCLSFVARAADPVAFVSDIRGDVVMTGAGRP
                     PFLAELLPGSRLVLGSEASAAVMYVVSGEEFTLEGPGEFVVTGTGVKAITGPAPSRRS
                     PALRVSASVVAQSSRTATASLRMRSAPVPKAERTGPLYPVNARIATLQPTLRWSGEAG
                     TTYTVVVTSAAGKEVFRGGTKGPSLRLPSRLVAGQGYSWTYSAATAAPGDSRFETLAS
                     DAIATAEKARSAAKTFADRVLLALVLQDLGAAQDAREVWAQLAAERPDIPELAGLSR"
     gene            5404..>6760
                     /locus_tag="VFV55_00905"
     CDS             5404..>6760
                     /locus_tag="VFV55_00905"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX4942876.1"
                     /translation="MTSTSLRTLTAIAAVVLLAFFASVAKAEGRVALLIGNNAYPSSP
                     LRNAVNDSRDLGAALRELGFRTIVKENTSRAEMIAALQEFGKALEGAEAALFFYAGHA
                     MQFKDRNYLIPIDAVMGSEDDVTFFSVEVSQVFDRMERARTRFNFLILDACRDNPFRD
                     TFKVSASGLAQMSGPSGTLIAYATAPGATAADGFGRNGVYTGHILQNIRIPEMPVEVL
                     FRKVREGVERDTKRLQTPWELSSIKGDFVFNLTGGRAVAGNGQAAGGTSADTAAQFEL
                     QFWKSVQDSTRADDIQAYLDKYPKGVFAPLARSRIDALLGRSRVAAATAPAGDARSAP
                     SVAAPSSPAAAPSTTVPDQVAATSPSASTTPAAAPRAADTTSGESPAVVAVIAPPAAS
                     STTSAQGAAAPVTSAAPAPKPRDDQPGREIAPGIREVTFADGAVYVGAMRGIQLHGKG
                     QY"
BASE COUNT         1094 a         2314 c         2257 g         1095 t
ORIGIN      
        1 cacgccagtg gcatcgccgg gtagctatat gcggaagaga taaccgctga aagcatctaa
       61 gcgggaaact tgcctgaaga tgagatctcc cgggggtcta acccccctga agggtcgtcc
      121 gagaccagga cgttgatagg ccgggtgtgc aagggcggta acgccttgag ctaaccggta
      181 ctaattgccc gtgcggcttg actctataac tctgcagggc ctgtgcgcgc taacgcgccg
      241 aggcagtacc aaccccaacc ttcgacattc ccgagcaagc gcgtggccgc ccgccaagag
      301 gcggccacga caccgaattc ctggcgacca tagcgcgttg gccccacccc ttcccatccc
      361 gaacaggacc gtgaaacgac gtcgcgccga tgatattgcg gattgcccgt gagaaagtag
      421 gtcatcgcca gggccttaca aacgaacgcc cgaccccaaa aggtcgggcg ttcgcatttg
      481 caggccgacc ttgcgcgccc ggggccgcga gcgataacct cctgcgacag tccgaagagg
      541 agaccgcctt gaaagcacgc gccgctgtcc ttcacaccgt cggtgcgccg ccgccctatg
      601 cgcaaagccg gcccttgcgg atcgaggaga tcgagatcgc gccgcccggg ccaggagaag
      661 tgctggtccg cgtgcacgct gccggcctat gccactcgga tctttcggtg atcaacggcg
      721 accggccgcg tcccgttccg atggcgctgg gccacgaggc ggccggcgag gttgtcgagt
      781 gcgggccgct cgttgccgat ctccagccag gcgaccacgt ggtgatggtc ttcgtgccga
      841 gttgcgggca ttgcgtgcct tgcatggaag gccgccccgc gctctgcgag ccgggcgccg
      901 cctcgaatac tgcgggaatg cttctcggcg gcggcagcag gctctcgatg gccggcaagc
      961 ccgtctatca ccacatgggc gtctcggcgt ttgccgacca cgccgtcgtg tcgcgccgct
     1021 cgctggtgaa ggtcgatccc acgcttgcct tcgacgaggc tgcgctcttc ggatgcgcgg
     1081 tcctgaccgg cgtcggcgcc gtcgtgaaca ccgcaaagat gacgccgggc accacggctg
     1141 ccgtggtggg actcggcggc gtcggcatga acagcatcct cgccgcttcg ctgtcgggcg
     1201 cgcgtgtcct cgtcgccgtc gatctcttcg acgacaagct ggaactcgcc aggtcgctcg
     1261 gggcgacgca cgccgtaaac gcccgggcgc ccgacgcggt cgagcaggtt cgcgaagcca
     1321 gcggcggcgg cgtcgacttc gccttcgaaa tggccggctc cgtgcaggcg atggaactgg
     1381 cctatcgcgt cacgcgacgc ggcggaacta cggtgacggc cgggctgccg cccccggatc
     1441 accgatggcc cctgcagcat gtaaacctca cggcggagga gcgcaccgtc aagggaagct
     1501 acatcggctc gtgcgtgccg gtgcgcgaca ttccgcgcta tatcgcgctc tacaaggggg
     1561 ggcgccttcc cgtggacaag ctgatgagcg accacctgaa gctcgaggac atcaacatcg
     1621 gcttcgaccg cctcgccagc gggcgggtcg tccggcaggt cgtgatgctc taggtcccgg
     1681 gaacgagccg atcgccatcg atggcgacgt caagcgcacc cctgcctgaa aaggcgacgc
     1741 aggtccgttc cctggccgcg ttcgcgcgcc ggctgtggac ccggccacgc tcgcgaagcg
     1801 ccgcgctgta cctgttcgtg gttctgctcc tggccgtcgc gagccgcgtt cccgatggcg
     1861 acgacgcgcc cctcgcccga ctcgagcgca cggccttcga ctggcagatg cagatgctgc
     1921 gcgagtactg gccgcgcccg atcgcgaacg acgtcgtcct ggtcgggatc gacgagggaa
     1981 gcgaagagat cttcaccgag ccggtggcgc tctggcaccg gcacttcgcc cgcctcctcg
     2041 tcgcgctggc cgatgcgcgg ccgcgcgcca tcggcatcga cgtggtgccg cccgagcgtt
     2101 cgtacgacga catcgtgccc ggctatgacc ttgccctctt tcgcgccatc cgcgacgccc
     2161 ggcagaagac cgcaaccgtc tttgcgttga ccgtcgaccg cgagggccgc gctgcgcgca
     2221 tgcacccgac gtttgcccgg gccctcggcg aggagggcct cggcatagac cagcaattgc
     2281 gcgaccccga ttcggtctcg cggcgattca gcgaagcgga cctcggcgga ccccaggcag
     2341 cgcggacgct ggtcgggcag atgctgcgcg ccatgggaat gcccgtcgat gccggctaca
     2401 tcgactacag cgtcggctcg cgcgtggagt acatcccgat gcaagacgtc atcgcctgga
     2461 aggagcaggg tgacggcgcg cggatcgaaa aggccctcgg cgggcggatc gtgctcgtgg
     2521 gttacgtcat gaagcgcgcg gaccgatggg aactgcctgt ccggctggtc gacatcgaca
     2581 aggccgacgg gcgcgcgcga ttcagccagc ctggcgtcat tacccacctg caggtgctcc
     2641 gcagccacct ggcgagcggc ctgcttaagc cgatgacgca ggagcttcgg tgggcgctct
     2701 gcctcctcgc cgccggcctc gcgttcctgc gcgtcaggca gacaatcgcg ttcgcggtag
     2761 tgctcgcgct taccgcagtc atctgcgccg ccggcctgtt cgcaatccgt gcaaaccagg
     2821 tgctgttgcc ggtcgcgtcg atcgtcttca cgctgtgggc cggcttcacg acgcgggcga
     2881 tcgcggacgg catcgagaac acgctggagc ggggccggct tcgccggaca tttgccgggc
     2941 aagtcagccc cgcggtgatg aacgagatgc tggccggcag cctctccccg ggcgtgagcg
     3001 gccagctcgc cgaagtatgc atcctgttca gcgacatccg cgacttcacc gcgctgtccg
     3061 agcgcatgcc gccggtcgtg gtgacgaccg tcctgcaacg ctatttcgac cgcatggtcc
     3121 aggccgtcca ccggcacgag ggcaccgtcg acaagttcat cggcgatggg atgatggtcc
     3181 tgttcggggc gccgcgacga tccgcagatc catgcggcga cgccgtgaaa tgctcgctgg
     3241 ccatgatgtc cgggctcgac gacctcaacg cggagttcct ctcggagggc ctcccgacgc
     3301 tcacggtcgg cataggcatc aacttcggcg tcgtcacggt cggcaacatc ggttcctcgg
     3361 aacggcacaa ctactcggcc atcggtgacg ccgtgaacgt cgccgcgcgc ctggaggggc
     3421 tgaccaagga actcgggcgt aagatactta tttccgacgc cgtggtgggc cggatgggtc
     3481 aggggttcca gttcgacccg ctgggaagcc acaaggtcaa gggacacagc ccggtcaacg
     3541 tctggggcat ccgcacggcg agacccgcgc ctgcggcgaa atgacagagg tccacatgaa
     3601 gaaagtcctt cgcgcactct tcgcattggc cgccaccctt gccgttccct gcgcccacgc
     3661 tgcaagcggc atcgcgttca tcaccgatct caagggggaa gtcgcggtcg acggtacgcc
     3721 ccgcccgctg ctcctgagcg agctggccag gggccagcgg atcattgtcg gcacggaggc
     3781 ccaactcgcg gtgatgttca tccagtcggg caaggagtac gtgctgaagg ggccggcgga
     3841 ctacgtggtc ggcgaacgcg agatcaccgc cactcatggc atgccgcccg cggcccggga
     3901 aactccctgg cgcgcaagca accaggtgct cgtcaaggtg gcacagactt cgggggccag
     3961 tatccgcatg cgttcgctcg ccccggtcaa gtccgacacg aagccgcggc tcgattttcc
     4021 gacgcggggt gcggtggcga cactgcaacc gaccctgcgc tggtcgaacc tgcccgacgg
     4081 caaggtcgag gtcgcgatcg cggtcgccgg gaaggaagac aagccccttg cgaaggccaa
     4141 ggtatcgggg gggagtcacc gttttccggt gaagctccgg cccgacacgg agtatgcgtg
     4201 gacggtgtcc ttcgagggcc gcgagatcgg ctcggccacg tttcggacgc tgcctgccgc
     4261 cgcgctgcag aacgcggaac agcgacgccc ttcggagaag gccgaattct ctgaccgcct
     4321 gctttacgcg cttctgctcc aggaaatcgg ggcgacccag gaggcgcagg aggcctgggg
     4381 caagctggcc gaggaacgca ccgacctgcc ggagctcgcc ggcctcgcga agtagcccgc
     4441 agaaaagctc tcgcgagttt cccatggcgc acctcacgcg gtcgttcctc gtcgggttcc
     4501 tcctctgcct ttcgtttgtt gcgcgcgcgg cggatccggt cgcattcgtg tccgacatca
     4561 ggggcgacgt cgtcatgact ggcgcgggcc ggccgccctt ccttgccgaa ctgctcccgg
     4621 gatccaggct cgtcctcgga tcggaggcga gcgccgcagt catgtatgtc gtgagcgggg
     4681 aggaattcac cctcgaggga ccgggagaat tcgtcgtcac cgggacgggc gtgaaggcca
     4741 tcacggggcc ggcgccgtcc cgccgcagcc cggcgttgcg tgtgagtgcg tcggtggtcg
     4801 cccaatcctc caggacggca accgcgagct tgcggatgcg cagcgcgcct gtcccgaagg
     4861 cagagcggac cggcccgctc tatccggtga atgcccgcat cgccaccctt cagccgacgc
     4921 tgcgctggtc cggcgaagcg ggcaccacgt acacggtggt ggtgacgtcc gctgccggca
     4981 aggaagtgtt ccgcggcggc acgaagggcc cctcgcttcg cctgccgtcc cggctggtcg
     5041 cgggccaggg ctattcgtgg acctattccg ccgcaacggc tgcgccaggc gattcccgtt
     5101 tcgagaccct ggcttccgac gcgatcgcca cggcggaaaa ggcgcgctcc gcagcgaaga
     5161 ccttcgccga ccgcgtcctt ctcgccctgg tcctccagga tctcggcgcg gcccaggatg
     5221 cgcgtgaagt ctgggcgcag ctcgcggccg agcgtcccga tattcccgag cttgccggcc
     5281 tgtcgcgcta gcgctcgccc ggggcacggg ggcggcgccc cgcgcacggc gtcttgggcg
     5341 ataatttgcg atccggcccg gcgccttctt gcgcgacgac cctggacaac gatgacaacg
     5401 gggatgacct cgaccagcct gcgcacactc accgctattg cagcggtcgt gcttctcgcc
     5461 ttctttgcgt cagtcgcgaa ggcggaaggt cgcgtggcgc tcctcatcgg caacaacgcc
     5521 tatccctctt cgccgttgcg caacgcggtg aacgattcgc gcgacctcgg cgctgcgctt
     5581 cgcgaactcg gcttcaggac gatcgtcaag gagaacacgt cacgcgcgga aatgattgcc
     5641 gcactgcagg aattcgggaa ggccctggag ggcgccgagg ccgccttgtt cttctacgcc
     5701 ggccacgcga tgcaattcaa ggaccgcaac tacctcatac cgatcgacgc ggtcatgggg
     5761 tccgaggacg acgtcacgtt cttctccgtg gaagtgtccc aggtcttcga ccgcatggag
     5821 cgcgcgcgca cgcgattcaa cttcttgatc ctcgacgcct gccgcgacaa cccgttccgg
     5881 gacacattca aggtgagcgc atccggcctc gcgcagatga gcggcccgtc cggaacgctc
     5941 attgcatacg ccaccgcgcc cggtgcgact gcggcggacg gtttcggacg caatggcgtg
     6001 tatacggggc acatcctgca gaacatcagg attcccgaaa tgccggtgga agtcctcttc
     6061 cgcaaggtgc gcgagggcgt ggagcgcgac accaagcgcc tgcagacgcc ttgggagctt
     6121 tcctccatca agggcgactt cgtcttcaat ctgacgggcg gccgggcggt cgcgggcaat
     6181 ggccaggccg ctggcggaac ctccgccgac accgcggcgc agttcgagct gcagttctgg
     6241 aaaagcgtgc aggattcgac gcgcgccgat gacatccagg cgtatctcga caagtacccc
     6301 aagggggtct tcgccccgct ggcccgcagt cggatcgatg ccctcctcgg gcgcagtcgc
     6361 gtggcggcag cgaccgcgcc cgccggggat gcacgatcgg cgccctcggt ggctgcaccg
     6421 tcaagtcctg cggcagcccc ttcgacaacg gtccccgacc aggttgctgc cacctcgcct
     6481 tccgcgagca cgacgcccgc tgcagccccc agggctgccg atacgacttc aggagaaagt
     6541 cccgcggtcg tggctgtgat cgcaccgcct gcggcatcct cgacgacgtc ggcccagggc
     6601 gccgcggcgc cggtgacgtc cgcggcgcct gcgccgaaac ccagggacga tcagcccggt
     6661 cgcgagatcg cgccgggcat ccgcgaggtc acgttcgcgg atggggcggt ctacgtgggc
     6721 gcgatgcgcg gcatccagtt gcacggcaag gggcagtaca
//
LOCUS       DASUEV010000029         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_123180, whole genome shotgun sequence.
ACCESSION   DASUEV010000029 DASUEV010000000
VERSION     DASUEV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123180"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFV55_00910"
     CDS             <1..580
                     /locus_tag="VFV55_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4942877.1"
                     /translation="VALFAAAREAGCYERLAILCIQETLRAFSRHGSRGLLFLNMSPR
                     LVQRPGFDPLRARRFLEDLELSPANVVIELTEDFPAIDLRHLRESLLLYRSMGFRVAL
                     DDLGEGFSTLRLWSELRPEFVKADKHFVNGIARDAVKRQFLRSIHHIARRCGSQVIAE
                     GIEVAEDFRAARRMGIALAQGWYIGRPGLMLA"
     gene            complement(590..1189)
                     /locus_tag="VFV55_00915"
     CDS             complement(590..1189)
                     /locus_tag="VFV55_00915"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4942878.1"
                     /translation="MPEFARSDPALPEFWEMRYRAAFTPWDAGAVPARLREHVARHRP
                     AGRVLVPGCGSGHDVRYLAEAGLDVQGIDFSPAALAAALPVLGPFADRVREADFFGPG
                     LEGPWSLVYERAFLCSLPRRLWTNWAARVAQLAAPGGLLAGFFYFDEGQRGPPFPLHG
                     QAELDGLLAEHFDRVEDLPVEDSVEVFAGKERWQAWARK"
     gene            1258..2709
                     /locus_tag="VFV55_00920"
     CDS             1258..2709
                     /locus_tag="VFV55_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4942879.1"
                     /translation="MLARPLLTAAILAVSSCTPIPETGHGTMKLADCRVAGLDRIARC
                     GSQQVWEDRASRQGRRIEIRVAVIPARRRDAEPDPIVVLAGGPGQGAIALAPQVTTLF
                     ARLNDSRDLVLVDQRGTGGSHPLDCEPEEDAGLQSLFEDALPEKMVKDCLASLDADPR
                     HYATSTAVADLDEVLGALGYARVNLWGGSYGTRVALEFMRRHPGRVRTATLDGVAPMG
                     MKLPLSFVADGEAAFDRLVADCESEAACRRSYPGLRGEVSGLRARLARRPARVAIVDP
                     VTGEPQAVRVTENILLSGLFRPLYAPELASLLPLAITRASDGDFNPLLAQNLELAEDI
                     ADNLSVGMHLSVICSEDVPRITPGDLAAASRGFFGRALVDDFLRACRHWPRAQLPADF
                     YEPVRSMAPVLILSGGIDPATPPRHGEAVAAGLPNARHAVAPHLSHGVSGHGCAPRLI
                     EKFVRSADARSLDTTCLARIPRPLFVLPVGTLP"
     gene            2706..>3124
                     /locus_tag="VFV55_00925"
     CDS             2706..>3124
                     /locus_tag="VFV55_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4942880.1"
                     /translation="MIAVDSLAKRFGPVEAVRQVSFDAADGSVTGLVGPNGAGKTTTL
                     RMISTLLAPSSGTVSVDGIDAARAPLTVRARIGTLSDARGLYSRLTARENITYYGELR
                     AMTRPGIADSIARLASFLEFEALLDRRSEGFSQGERM"
BASE COUNT          449 a         1126 c         1084 g          465 t
ORIGIN      
        1 ggtcgcgctg ttcgccgccg cgcgcgaggc gggttgctac gagaggctcg cgatcctctg
       61 catccaggag acgctgcgcg cgttctcgcg gcacggctcg cgcggcctgc tctttctcaa
      121 catgtcgccg cgattggtgc agcgacccgg attcgatccc ctccgcgcca ggcgcttcct
      181 cgaagacctc gagctcagtc ccgcgaacgt ggtgatcgag ctcaccgagg actttcccgc
      241 gatcgacctg cgccacctgc gcgaatcgct gctgctctac cggtcgatgg gcttccgcgt
      301 cgcgctcgac gatctcggcg agggcttctc gaccctgcgc ctgtggtcgg agctgcggcc
      361 ggagttcgtg aaggccgaca agcatttcgt gaacggaatc gcccgcgacg ccgtcaagcg
      421 gcaattcctt cgctcgatcc atcacatcgc gcggcgctgc gggtcgcagg tcatcgcgga
      481 aggcatcgag gtcgccgagg attttcgcgc ggcccggcgg atggggatcg cgctggcaca
      541 ggggtggtac atcggccggc ccgggctgat gctggcttga ccgggcggcc tacttgcgcg
      601 cccaggcctg ccaccgctcc ttgccggcaa agacttcgac cgagtcctcc acggggagat
      661 cctcgacccg atcgaaatgc tccgccagaa gcccgtccag ctccgcctgg ccgtgcaagg
      721 gaaacggcgg gccgcgttgc ccctcgtcga aatagaagaa gccggcgaga agtcctcccg
      781 gcgcggcgag ttgcgcaacg cgcgcagccc agttcgtcca gagccgccgg ggcaacgagc
      841 acaggaaggc acgctcgtag accagggacc agggtccctc gaggcccggg ccgaagaaat
      901 cggcctcgcg cacacgatcg gcgaacgggc cgaggacggg aagcgcggcg gccagcgcgg
      961 ccgggctgaa gtcgattccc tgcacgtcga gcccggcctc ggcgagatag cgcacgtcat
     1021 gcccggagcc gcagccgggt accagcaccc ggcctgcggg gcgatgcctc gcgacgtgct
     1081 cgcgcagccg cgcgggcacg gcccccgcgt cccacggcgt gaacgcagcg cggtaacgca
     1141 tctcccagaa ttccggcagg gcgggatcgg agcgggcaaa ctcgggcacg gccatatccg
     1201 ggatggtagc gcctaacggc tatacttcgc gctctcccga cgcttcggcg gcccgtcttg
     1261 ctcgcgcgac ctctcctgac tgcggcgatt ctcgccgttt cctcctgcac cccgattccc
     1321 gaaaccggcc acggcacgat gaagcttgcc gactgccggg tggcgggcct cgaccgcatc
     1381 gcgcgctgcg gtagccagca ggtgtgggag gaccgcgcaa gccgccaggg caggcgcatc
     1441 gaaatccgcg tggcggtgat ccccgcgcgc cggcgcgacg ccgagcccga cccgatcgtc
     1501 gtgctcgcgg gcggcccggg ccagggcgcc atcgccctcg cgccgcaggt gacgacgctc
     1561 ttcgcgcggc tcaacgactc gcgcgatctc gtcctggtcg accagcgcgg aacagggggc
     1621 tcccatccgc tcgattgcga gccggaggag gacgcgggcc tgcagtccct gttcgaggat
     1681 gccctgccgg aaaagatggt caaggactgc ctggcgtccc tggatgcgga tccgcgccac
     1741 tacgcgacga gcacggcggt cgccgacctg gacgaggtgc tgggggcgct gggctacgcc
     1801 cgcgtgaacc tgtggggcgg ttcgtacggc acgcgggtgg cgctcgaatt catgcgccgc
     1861 caccccgggc gcgtgcgcac cgccacgctc gacggcgtgg cgcccatggg aatgaaattg
     1921 ccgctctcgt tcgtggccga cggcgaggcc gcctttgaca ggctggtcgc cgattgcgag
     1981 tcggaggcgg cgtgccgcag gtcctatccc gggcttcgcg gggaagtaag cgggctgcgc
     2041 gcccggctcg cgcggcgtcc ggcgcgcgtg gcgatcgtgg atccggtcac cggcgagccg
     2101 caggccgttc gggtgacgga aaacatcctc ctgtcgggcc tgttccgccc gctctacgcg
     2161 ccggagctcg ccagtctcct tccgctcgcc atcacgcgcg cgagcgacgg cgacttcaac
     2221 ccgctcctcg cgcagaacct cgaactcgcc gaggacatcg ccgacaacct gtcggtaggc
     2281 atgcacctgt cggtcatctg ctcggaggac gtgccgcgca tcacccccgg ggatctcgcg
     2341 gcggcctcgc gcggattctt cgggcgtgca ctggtcgacg acttcctgcg cgcctgccgg
     2401 cactggccgc gtgcgcaact gccggcggac ttttacgagc cggtgcgctc catggcgccg
     2461 gtgctcatcc tgtcgggggg gatagacccg gccacgccgc cccggcacgg cgaggcggtc
     2521 gcggccgggc tgcccaacgc gaggcacgcc gtggcgcccc acctctcgca cggcgtgagc
     2581 gggcacggct gcgcgccgcg cctcatcgag aaattcgtgc gcagcgcaga cgcccgctcg
     2641 ctcgacacaa cgtgtctcgc gcgcatcccg cgcccgctct tcgtgctgcc ggtgggcacg
     2701 ctgccgtgat cgcggtcgac agcctcgcca agcgcttcgg gcccgtggag gccgtgcgcc
     2761 aggtttcgtt cgacgcggcc gacggcagtg tgacgggcct cgtagggccc aacggcgccg
     2821 gaaagacgac gacgctgcgc atgatctcca cgctgctggc gccttcctcg gggacggtaa
     2881 gcgtcgacgg gatcgacgca gcgcgcgcac ctcttacggt gcgcgccagg atcggcacgc
     2941 tgtccgacgc gcgcggcctc tattcccgcc tcacggcacg agagaacatc acctactacg
     3001 gcgagctccg cgccatgacg cggccgggaa ttgcggattc gatcgcgcgg ctcgcatcct
     3061 tcctcgagtt cgaggcgctc ctcgaccggc gcagcgaggg cttctcgcag ggcgagcgca
     3121 tgaa
//
LOCUS       DASUEV010000030         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_9172, whole genome shotgun sequence.
ACCESSION   DASUEV010000030 DASUEV010000000
VERSION     DASUEV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9172"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFV55_00930"
     CDS             complement(<1..422)
                     /locus_tag="VFV55_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4942881.1"
                     /translation="MAVIGDRHREIERLLAERNRTERELRRVNRALRVRSECNQAIVR
                     AEAEPALLQAVCRILVEPGGYRMAWVGYAEADGQRRVWPMAMAGHETAYVERIKITWA
                     DDEHGQGPGGKALRTGVPQVNRDFRSNAYMTPWRVDALA"
     gene            complement(426..1427)
                     /locus_tag="VFV55_00935"
     CDS             complement(426..1427)
                     /locus_tag="VFV55_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX4942882.1"
                     /translation="MKLSVLDQSPAAAEKTQDFAIRESLALARHCEALGYHRYWVSEH
                     HNSASIVGTAPEILMAAIAATTQRIRVGSAGVMLPHYSALKVAEQFRVLEAIAPGRID
                     LGVGRAPGSDRLTAMALNPDPGAAENFPSQILEMDRWLRGEPLPEGHPFRPIHAHPMG
                     PTSPEIWVLGSSDYGAQLAAHLGLPYAFAYFFTDGRGTEEALAIYRAGYRPSARYPKP
                     VATICVWALAADTEAQARRLLVTREHWRVGFEKGLRTPLLSPEDAAACSYNEAERAII
                     GRLRENAIVGTAVQVAAQLEKLAAKFALEELVINTWTHDPAARHRSYELLARSFDLG"
     gene            complement(1424..1750)
                     /locus_tag="VFV55_00940"
     CDS             complement(1424..1750)
                     /locus_tag="VFV55_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX4942883.1"
                     /translation="MAAERDDALWLDARRQVSLDELAECSGISVEVLRELVEYGALSP
                     ADPEAGEWVFWADCVVSVRTAARVCHDLELETPVLALVLSYVEHIERLEAQVRELSAR
                     LGETPR"
     gene            complement(1740..2714)
                     /locus_tag="VFV55_00945"
     CDS             complement(1740..2714)
                     /locus_tag="VFV55_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX4942884.1"
                     /translation="MKYKDYYAVLGVARDASDAAIKSAYRKLAQKYHPDVTKDPKGEE
                     KFKEIAEAYQALKDPERRAAYDQLGSGYQPGQDFRPPPDWERQFHRGDSSGDGGFSFD
                     GVDLSDLFANFGGSSRRGGPGRGAMPIPGADYEVSVHLSLEEAHRGTQVDLNLSVPEA
                     DGEGRVRHVAKTVKARIPKGATDGQRLRLRGQGGKGANGGRNGDLYLNILLHPHPLFR
                     PTGHDLYLDLPLAPWEAALGASIDVPTLSGTVSLKVPPGTPSGRKLRFAGRGLAKPGG
                     GEGDLYAIVQIVNPTVLGERERALFGELAQASHFDPRGHFAGGGSNGG"
BASE COUNT          403 a          973 c          962 g          439 t
ORIGIN      
        1 gccagggcgt ccacgcgcca cggcgtcatg tacgcgttcg agcgaaaatc ccggttcacc
       61 tgggggacgc cggtgcgcag agccttgccg ccgggaccct ggccatgctc gtcgtccgcc
      121 caggtgatct tgatgcgctc cacgtacgcg gtctcatgac ccgccatcgc catcggccac
      181 acacgccgtt gtccgtccgc ttccgcgtaa ccgacccagg ccatgcggta gccgccgggc
      241 tcgaccagga tcctgcacac ggcctggagg agcgccggct cggcctccgc ccgcacgatc
      301 gcctggttgc actcgctccg gacgcgcagc gcgcggttta cccgtcgcag ctcgcgctcg
      361 gtgcggttgc gttccgcgag cagccgctcg atctcgcgat gacggtcgcc gatgacggcc
      421 atggctcagc ccaggtcgaa cgagcgcgcc agcaactcgt aggaacggtg gcgcgcggcg
      481 gggtcgtggg tccaggtgtt gatgaccagt tcctccaggg cgaatttcgc ggccagcttc
      541 tcgagttgcg ccgccacctg caccgccgtg cccacgatgg cgttctcgcg cagtctcccg
      601 atgatcgcac gctccgcctc gttgtacgag caggcggccg cgtcttcggg ggaaagcagg
      661 ggcgtgcgca agcccttttc gaagcccacc cgccaatgct cgcgcgtcac gagcaggcgc
      721 cgcgcctgcg cctcggtgtc ggcggcaagg gcccagacgc agatcgtcgc cacgggcttc
      781 ggatagcgcg cgctggggcg gtagccggcg cgatagatcg ccagcgcctc ctccgtgccg
      841 cgcccgtcgg tgaagaagta cgcgaaggcg taaggaaggc ccaggtgcgc cgcgagctgc
      901 gcgccgtagt cggaactgcc gagcacccag atttccgggc tcgtgggccc catggggtgg
      961 gcatgaatgg gccggaaggg atggccctcg ggcaggggct cgccgcgcag ccaccggtcc
     1021 atctccagga tctggctcgg gaagttctcg gccgcgcccg gatcgggatt cagcgccatc
     1081 gccgtgaggc ggtcggagcc cggcgcacgg cccacgccga ggtcgatgcg gccgggcgcg
     1141 atggcctcga ggacacggaa ctgctcggcg accttgaggg ccgagtaatg cggaagcatc
     1201 acgccggcgc tgccgacgcg gatgcgttgc gtcgtggcgg cgatcgcggc catcaggatc
     1261 tccggggcgg taccgacgat gctcgcgctg ttgtggtgct cggataccca gtaccggtga
     1321 tagcccaggg cctcgcagtg cctggccagt gccagcgact cgcggatggc gaaatcctgg
     1381 gtcttctccg ccgccgcggg agactggtcc aggaccgaaa gcttcatcgc ggcgtctcgc
     1441 ccagccgggc gctcagctcg cgcacctgcg cctccagccg ctcgatgtgc tcgacgtagg
     1501 aaaggacgag ggcgagcacg ggcgtctcca gctcgaggtc gtggcacacc cgcgccgcag
     1561 tgcgcacgct cacgacgcaa tccgcccaga agacccactc gcccgcttcg gggtcggcgg
     1621 gcgaaagcgc gccgtactcc acgagttcgc gaaggacctc caccgagatc ccggaacact
     1681 ccgccagttc gtccagcgaa acctggcgcc gcgcgtccag ccagagcgcg tcgtcgcgtt
     1741 cagccgccat tcgatcctcc tcctgcgaaa tggccgcgcg gatcgaagtg cgacgcctgc
     1801 gcgagttccc cgaaaagcgc cctctcgcgc tcgcccagga ccgtcgggtt gacgatctgc
     1861 acgatggcgt aaaggtcgcc ctcgcctccg cccggcttcg ccagcccccg gcccgcgaag
     1921 cgcagtttcc tgccggacgg cgtgcccggg ggaaccttga ggctcaccgt gccggaaagg
     1981 gtcggcacat cgatcgacgc gcccagtgcg gcttcccagg gcgcgagcgg caggtcgagg
     2041 tacaggtcgt gcccggtggg ccggaagagc ggatgggggt gcaacaggat gttcaggtac
     2101 aggtcgccgt tgcggccgcc gtttgcgccc ttgccgccct gcccgcgcag tcgcaaccgc
     2161 tggccgtcgg tggcgccttt cgggatgcgc gccttgaccg tcttcgcgac gtgccgcacg
     2221 cgcccctctc catcggcttc cggcacgctc aggttcaggt ccacctgcgt gccgcgatgg
     2281 gcctcttcca gcgagaggtg gacgctgacc tcgtaatcgg cacccgggat gggcatggcg
     2341 ccacggcccg gcccgccgcg ccgcgaactg cccccgaaat tggcgaagag gtcggagagg
     2401 tccactccgt cgaaggaaaa tccgccgtcg ccggacgagt cgccgcggtg gaactggcgt
     2461 tcccagtcgg gcggcgggcg gaagtcctgc cccggctggt aaccgctgcc gagctggtcg
     2521 taggccgcgc gcctttccgg gtccttgagc gcctggtagg cctcggcgat ctccttgaat
     2581 ttctcctcgc ccttgggatc cttggtgacg tccgggtggt acttctgcgc cagcttgcga
     2641 taggccgact tgatggccgc gtcgctcgcg tcgcgcgcaa ccccgagtac cgcgtagtag
     2701 tccttgtact tcatggtgaa gagtttaccg gctcatcggg aaaggggtgc ggtgggagtc
     2761 gagcgcaagc gcgggca
//
LOCUS       DASUEV010000031         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_581928, whole genome shotgun sequence.
ACCESSION   DASUEV010000031 DASUEV010000000
VERSION     DASUEV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581928"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFV55_00950"
     CDS             <1..358
                     /locus_tag="VFV55_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942885.1"
                     /translation="GEDWERFMDALDDRRVEITARAVRDLLADTLVTLPTLVEREAVP
                     SLHFWFATFSGLRRELFPRLMTAHAAWKAGAGPQGLRIAIDEGRAHWLRVARELAADA
                     QAARRYALEPGVITLA"
     gene            complement(355..684)
                     /locus_tag="VFV55_00955"
     CDS             complement(355..684)
                     /locus_tag="VFV55_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX4942886.1"
                     /translation="MGYVIGGIEKEADADGYLKEPDFSEEAVQVIAAADGIALTEDHW
                     KVVNYLRDEFRDKGHTPNFRNMVKDLQEVLPGCDSKSLYDLFPLGPAKQGCKVAGLPQ
                     PYGKGGY"
     gene            complement(772..1185)
                     /locus_tag="VFV55_00960"
     CDS             complement(772..1185)
                     /locus_tag="VFV55_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942887.1"
                     /translation="MTALARTAPDQERYARVLAIGTNAGLAMLVILFGIYVLGVVDPH
                     VPHERLPDLWHLSAGNLLEHSGVEAGWGWTAIIHRADTLTLVGIVTLAICSVPCLLAV
                     MPIYWASGQRTLCAICALEVAVIVLAASGVVGGGH"
     gene            complement(1193..1705)
                     /locus_tag="VFV55_00965"
     CDS             complement(1193..1705)
                     /locus_tag="VFV55_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX4942888.1"
                     /translation="MRATRVIGAAAFAAAAGAAVAAPELMSEAWAKAACDAWNADATL
                     TAGLYESGWAANDKKRGYKALQVVRKDCTSSPKVELRISAKDGKALCVSGGRSSGALD
                     LDVDYSMTAETRRWIEMGKGEYGPMRAMMFGRLGFDGPMGEAMGNMGPFESFLLLVGK
                     VPGDTAACPK"
     gene            complement(1742..2695)
                     /locus_tag="VFV55_00970"
     CDS             complement(1742..2695)
                     /locus_tag="VFV55_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4942889.1"
                     /translation="MKRIVLLSAAAAIFLAAPEVATAADAANAQAGSGTVLPWWAWPL
                     ILFGVCFLLGIVAVPAGVGGGVLFVPIVGGFFPFHLDFVRGAGLLVALASALSAGPSL
                     LQSGLASLRLALPLAVVASASSILGALIGFALPTQAVQTALGGTILAIVVLMAMSKRS
                     EFPQVQAPDALSAALGMNGVLVEAGTGRQVHWQVHRTPAGLATFFVIGILAGMFGLGA
                     GWANLPALNLLMGAPLKVSAGTSSFILSLVDSSAAWVYLNKGAVLPMIAVPSVIGMML
                     GAKIGAKLLRVLPAASVRKLVLALLAFAGMRALLKGLAVWP"
BASE COUNT          458 a         1003 c          910 g          393 t
ORIGIN      
        1 gggcgaggac tgggaacggt tcatggacgc gctggacgac cggcgcgtgg agatcacggc
       61 gcgcgcggtg cgcgacctgc tcgccgacac cctggtcacg ctgcccaccc tcgtcgagcg
      121 cgaggcggtg ccgtcgctgc acttctggtt cgcgactttc tcggggctgc gccgcgaact
      181 cttcccgcga ctgatgactg cccacgccgc gtggaaggcg ggcgccggcc cgcaaggctt
      241 gcgcattgcg atcgacgagg ggcgcgcaca ctggcttcgc gtggcgcgcg aactcgcggc
      301 cgacgcgcaa gccgccaggc gctacgcgct cgagcccggc gtgatcacgc tcgcctagta
      361 gccgcccttg ccgtagggct ggggcagccc cgccaccttg cagccctgct tggccgggcc
      421 cagcggaaag aggtcgtaaa gcgacttcga atcgcagccg ggaaggactt cctgcaggtc
      481 cttcaccatg ttgcggaaat tgggcgtgtg ccccttgtcg cggaattcgt cgcgcaggta
      541 gttcaccacc ttccagtggt cctcggtgag cgcgatcccg tccgcggccg cgatcacctg
      601 caccgcctcc tcactgaaat ccggctcctt gaggtagccg tccgcgtccg cttccttctc
      661 gatgccaccg atcacgtatc ccatccgtct ctccgtcgtt gaaatggccg gtccgttcca
      721 cggccaagaa aaatcgcaat tgcatcccgg caggccgcga cagcccgcgc cctagtgacc
      781 gcccccgacc acgccggagg cggccagcac gatcacggcc acctcgagcg cgcagatcgc
      841 gcacagcgtg cgctggcccg atgcccagta gatcggcatc accgcaagca ggcagggcac
      901 cgagcaaatg gccagcgtga cgatgccgac gagcgtgagc gtgtccgcgc gatggatgat
      961 cgcggtccat ccccagcccg cctcgacacc ggagtgctcg agcaggttgc cggcgctcag
     1021 gtgccagagg tccggcagcc gctcgtgcgg gacgtgcggg tcgacgacgc cgagcacgta
     1081 gatgccgaac aggatcacca gcatcgcgag ccccgcgttg gtgccgatgg caaggacgcg
     1141 cgcgtagcgt tcctggtccg gcgccgtccg cgccagcgcg gtcattcgtt cctcacttcg
     1201 ggcaggcggc ggtgtcgccg ggcaccttcc ccaccaggag caggaagctc tcgaaggggc
     1261 ccatgttgcc catcgcctcg cccatcgggc cgtcgaagcc caggcggccg aacatcatcg
     1321 cgcgcatggg gccgtattcg cccttgccca tctcgatcca gcgccgggtc tcggccgtca
     1381 tcgaatagtc gacgtccaga tccagcgcgc cggacgagcg gccgcccgac acgcacagcg
     1441 ccttgccgtc cttcgccgag attcgcagtt ccaccttcgg gctggacgtg cagtccttgc
     1501 gcaccacttg cagggccttg tagccccgct tcttgtcgtt ggccgcccag ccgctctcgt
     1561 acagcccggc cgtcagcgtc gcatctgcgt tccacgcgtc gcaggccgcc ttggcccagg
     1621 cctccgacat caactccggg gccgccacgg ccgcacctgc cgcggccgcg aaggccgccg
     1681 ctccgattac ccttgttgcg cgcatcatct tttcctcctg gtcgaatggg tcacgagttc
     1741 atcagggcca taccgcgagc cccttcagca gtgcgcgcat gccggcgaag gcaagcaggg
     1801 cgagcacgag cttgcgcacc gacgcggcgg gcagcacccg cagcagtttc gcgccgatct
     1861 tggctccgag catcataccg atcaccgagg gcacggcgat catgggcagc accgcgccct
     1921 tgttcaggta gacccacgca gccgagctgt ccaccagcga caggatgaag ctcgacgttc
     1981 ccgccgacac cttgagcggc gcgcccatga gcaggttgag cgccggcagg ttggcccacc
     2041 ccgcgcccag cccgaacatg ccggcgagta tcccgatgac gaagaaggtc gccagtccgg
     2101 cgggcgtgcg gtggacctgc cagtgaacct ggcgaccggt gccggcctct accagcacgc
     2161 cgttcatgcc gagcgcggcc gagagggcat cgggcgcctg cacctgcgga aattcggaac
     2221 gcttcgacat cgccatcagg acgacgatcg cgagaatggt cccgccgagc gccgtctgga
     2281 ccgcttgcgt gggcagcgcg aagccgatca gggcgccaag aatcgagctg gcggaggcga
     2341 cgacggccag cggcagcgca aggcgaaggc tggcaaggcc ggattgcagg agcgatggcc
     2401 ccgcggagag cgcgctcgcg agcgccacca gcaaccccgc gccgcggacg aaatcgaggt
     2461 ggaagggaaa gaaccccccg acgatcggca cgaagagcac gccccctccg acgccggccg
     2521 gcacggcaac gatgccgaga aggaagcaca cgccgaagag gatgagcggc catgcccacc
     2581 agggcagcac cgttcccgag cccgcctggg cattcgccgc gtccgcggcc gtcgcaacct
     2641 cgggcgcagc gaggaagatc gcggccgcgg ccgagagcag gacgatccgc ttcaccggcg
     2701 cttccgtccc gcctggtagg cgacatcctc gggcgcctcg gccatgccgg gatagggacg
     2761 cagc
//
LOCUS       DASUEV010000032         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_926302, whole genome shotgun sequence.
ACCESSION   DASUEV010000032 DASUEV010000000
VERSION     DASUEV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926302"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFV55_00975"
     CDS             <1..410
                     /locus_tag="VFV55_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942890.1"
                     /translation="HISFRAATSQIASGPQVLVKARISKWPGSCDDGPGQGLAQSGEP
                     DEKLNRHHPRLARQRRWHDGKTVKAAKDPFLTRLATYCNAAGIPAIAKAADDDDQDLP
                     RDSRTALPQTGLTGRIVHERGTRLRGRRRFDEQ"
     gene            592..1962
                     /locus_tag="VFV55_00980"
     CDS             592..1962
                     /locus_tag="VFV55_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX4942891.1"
                     /translation="MNDTVKYVLDEGRIPKSWYNITADLPKPLPPVLHPGTKLPIGPD
                     DLAPLFPMELILQEVSTEREIAIPGPVREVYRQWRPSPLYRARRLEKVLQTPARIYYK
                     YEGVSPAGSHKPNTAVAQAFYNKQAGVKRLITETGAGQWGSSLAFAGGVFGLEVEIFM
                     VRVSYDQKPYRRALMETYGARCIASPSSETHSGREILAKRPDHPGSLGIAISEAVEVA
                     AQRDDTKYALGSVLNHVLLHQTVIGQEALIQLEMANDYPDVIVGCTGGGSNFAGIAFP
                     FIGAQLRGGKKVRVVAVEPAACPSLTRGHYAYDFGDTAHLTPLTKMHTLGSTFTPPGF
                     HAGGLRYHGMAPLVSHLQELGLITSTAYHQIACFEAGVQFARTEGIVPAPEANHAVRA
                     AIDEAIKCREEGVSRAILFNLSGHGHFDMQAYIDYFDGKLKDINYDEKELAMALAGLP
                     SVAEPA"
BASE COUNT          378 a          734 c          664 g          296 t
ORIGIN      
        1 cccatatcag tttccgggcg gcgacaagcc agatagccag cgggcctcaa gtacttgtta
       61 aggcgaggat ttccaaatgg cccggatcct gcgacgacgg ccctggacag ggtctcgccc
      121 agtcaggaga acccgatgaa aagctcaatc gtcatcaccc tcgactcgcc cggcagcgcc
      181 gctggcacga tggaaagacc gtgaaagccg ccaaggaccc gttcctcacc cgcctcgcca
      241 cctactgcaa cgcggccggg atccctgcga tcgccaaagc cgctgacgac gacgatcaag
      301 acctgccgcg cgactcgcgg acggcgcttc cgcagacggg cctcacgggc cgcatcgtcc
      361 atgaacgggg tacccggctc cgtgggcgac gcaggttcga cgaacagtga ccacggcggg
      421 gtcaagttcg tcccgctccg catcctgatg cgcgaaaggt tcgcgtagca ggctctcctc
      481 ccagggccgg ctgatcggcg tcgttggccg gggaccggca ggccgatggc gtgacccggg
      541 cacgggtaaa ctgtggcccg ggcacgccaa gtccaatccg gagagagagc catgaacgac
      601 accgtcaagt acgtcctcga cgaaggacgc atcccgaagt cctggtacaa catcaccgcg
      661 gacctgccga agccgcttcc gccggtgctg cacccgggta cgaagctgcc catcggtccc
      721 gacgacctcg cgccgctttt tcccatggag ctgatcctcc aggaggtgtc cacggagcgc
      781 gagatcgcga tccccgggcc ggtgcgcgaa gtgtaccggc aatggcggcc ctcgccgctc
      841 taccgcgcgc ggcgcctcga gaaggtgctg cagaccccgg cgcgcatcta ctacaagtac
      901 gagggcgtgt cgccggcggg cagccacaag cccaacacgg ccgtggccca ggccttctac
      961 aacaagcagg cgggcgtgaa acgcctcatc accgagaccg gggcagggca atgggggagc
     1021 tccctcgcct ttgccggcgg ggtgttcggg ctggaggtgg aaatcttcat ggtgcgcgtg
     1081 tcctacgacc agaagccgta ccgccgggcg ttgatggaaa cctacggggc gcgctgcatc
     1141 gcctcgcctt cgagcgagac gcactccggc cgcgagatcc tcgccaagcg ccccgaccac
     1201 cccggcagcc tgggaatcgc catctccgag gccgtggaag tggccgcgca gcgcgacgac
     1261 acgaagtacg cgctgggcag cgtgctcaac cacgtgctcc tgcaccagac ggtgatcggg
     1321 caggaggcgc tgatccagct cgagatggcc aacgactacc ccgacgtgat cgtgggttgc
     1381 accggcggcg gctccaactt cgccggcatc gctttcccct tcatcggcgc ccagctgcgc
     1441 ggcggcaaga aggtccgcgt cgtggccgtg gagcccgccg cgtgccccag cctcacgcgc
     1501 ggccactatg cctacgactt cggcgacacg gcgcacctca cgccgctcac caagatgcac
     1561 acgctgggat cgaccttcac gccgcccggc ttccacgcgg gcgggcttcg ctaccacggc
     1621 atggcgccgc tggtctcgca ccttcaggaa ctgggcctca tcacctcgac cgcctaccac
     1681 cagatcgctt gcttcgaggc aggcgtgcag ttcgcgcgca ccgagggcat cgtaccggcg
     1741 ccggaggcca accacgcggt aagggccgcc atcgacgagg cgatcaagtg ccgcgaggaa
     1801 ggcgtctcgc gcgccatcct cttcaacctg agcgggcacg ggcacttcga catgcaggcc
     1861 tatatcgact acttcgacgg gaagctgaag gacatcaact acgacgagaa ggagctcgcc
     1921 atggcactgg ccgggctgcc ttccgtggcg gagccggcct gacgcggcga ggacaaccgt
     1981 gcgcagtgac cccctctatc tcgcgataga ccagggcacg cacgcaagcc gcgccgtggt
     2041 cctggatatc cacggcggcg tggtggccac cg
//
LOCUS       DASUEV010000033         6627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1040876, whole genome shotgun sequence.
ACCESSION   DASUEV010000033 DASUEV010000000
VERSION     DASUEV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040876"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1020
                     /locus_tag="VFV55_00985"
     CDS             76..1020
                     /locus_tag="VFV55_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX4942892.1"
                     /translation="MNATAKPAILVTAADLAPQALALLGDFDIVFAGRTPDEDAIASL
                     CAKHQPVAIIVRYGKIPARVMDASARLRVISKHGSGTDTIDKVAAAQRGIEVKAAAGA
                     NADAVAEHSWALILACAKGVVTLDGRMRAGFWDKATHKSFELKGKTLGVVGLGAIGRR
                     VAQIGLAFGMRVVGHDPFVTEAPAGVAAATLDELFAASHVVSLNCPLTDENRQMVNRT
                     SIAKMRDGAILVNTGRGGLVDEQALLEALASGKLRAAGLDAYSTEPLVGEHPFRAVPN
                     VVLSPHVGGVSEDAYVNMGTAAARNVLDALSGAPRQAA"
     gene            1154..2104
                     /locus_tag="VFV55_00990"
     CDS             1154..2104
                     /locus_tag="VFV55_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4942893.1"
                     /translation="MKRREFLVLGGAMAASIALPARAAYPEKPVRMIIAFSPGGESDI
                     GARLQQGVFRKLYGQEMVIESKPGAGGALAWSTLNTYPGDGYTVMGTNLPHIVLQPLE
                     GNVQYRTDDVTNVHFYQYTPDAIIVRNESPFKTYQDLLKAAKEGGDKVTFAGSGTNSA
                     NHLAHTRLNKLMGTKTTYVPFKGTGDLLSSVLGGHVSAAMSYNTLAISQKNKMRMLAV
                     ASERRLAGFPDVPTFKELGGDWVDGAYRGVGVPKSTPEDLRRRISEMFSEINRDAEFR
                     QKMADGGFELVDITYDKMAAFVAERTKVYLESAKLMGLVK"
     gene            2262..2753
                     /locus_tag="VFV55_00995"
     CDS             2262..2753
                     /locus_tag="VFV55_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4942894.1"
                     /translation="MKHLIKNPRDFWAGVMFLALGVGFAGIAFTYKLGTAARMGPGFF
                     PFFLGIIVAALGVGILIAAMNPKNAGQHVDKFHWGPIFWVLMPIVVFGMLLKILGMMI
                     MGLFVVILSSIGSEEFKLRPTVYLAFGLVIFCSAVFVGGLKLPIPLCPSFEFFDQFAL
                     CRV"
     gene            2761..4266
                     /locus_tag="VFV55_01000"
     CDS             2761..4266
                     /locus_tag="VFV55_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4942895.1"
                     /translation="MELLANLELGFGVALAGTNILYCLIGVMLGTLIGVLPGIGPVAT
                     IAMLLPATFVLPPVSALIMLAGIYYGASYGGSTTAILVNLPGEVSSVVTCLDGYQMAR
                     QGHAGKALAIAAIGSFFAGTVATVLVAAFAPPLAELALKFGPADYFSLMVLGLIAAVV
                     LAHGSLIKAVAMIVLGLLIGLIGTDVNSGVARYSFGLPELIDGVGIGPIAMGVFGFGE
                     IIRNLEHGQKRDVFVKRVTGLMPSWIDLKMSFWPIVRGTALGSALGILPGGGPILGSF
                     SAYTLEKKLAKDPSRFGKGAIEGVAAPESANNAAAQTSFIPLLTLGIPSNPVMALMIG
                     AMIIHGIQPGPQIMTANPQLFWGMIASMWVGNLLLVVLNLPMIGMWVKLLTLEYRFLY
                     PAILLFCCIGVYSINNTWMDVILAAGFGFIGYLFGKLDCEAAPLLLGFVLGPMMEENL
                     RRALLLSRGDPAVFVTRPISASLLVVAALLLVVVLAPSVRKKREETFVEEV"
     gene            complement(4263..4937)
                     /locus_tag="VFV55_01005"
     CDS             complement(4263..4937)
                     /locus_tag="VFV55_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX4942896.1"
                     /translation="MHAPQALVFDAYGTLFDVHSVTRLADSLFPGRGAALSQAWRSKQ
                     LEYTWLRSLMGRYDDFGAVTEGALDWALESLGLAARAGDRTALLLAYRTLGTFPEVRG
                     ALERLGERRPLAILSNGHPDMLDAVVDHNELRIFFGERVFSVHEAGVYKPDPRVYAIA
                     TAGLELPADAIGFVSSNGWDAAGARSFGFTAFWVNRGKAPVDRLGSPPDAVVEDLAGL
                     ARLLAA"
     gene            5022..6608
                     /locus_tag="VFV55_01010"
     CDS             5022..6608
                     /locus_tag="VFV55_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX4942897.1"
                     /translation="MFTWDFPYPSQRMPLLAKNVVATSQPLAAQAGLQALAKGGNAVD
                     AALAAAITLTVVEPTSNGIGSDAFAILWDGEKLVGLNASGRAPAGWTPARFKGREAMP
                     VHGWDAVTVPGCVSAWVALSQRYGKLHFSDLFQAAIRYARDGYMVSPITAANWARQAP
                     NFTGFSEFAWTFLPKDRAPRAGERFYCPQQAETLEEIAATKGESFYRGKLAERIALAS
                     QADEGAHTLADFAAHRCDWVEPLSVEYRGYRLHEIPPNGQGIVALMALGILRQFDLAA
                     LPVDRADSLHLQLEAMKLAFADAYRYVSDPATMEFDAKMLLADDYLAGRARAIDMKRA
                     QDPPPGEPRAGGTIYLTTADASGMMVSYIQSNYMGFGSGMVVPGTGISMQNRGAGFSL
                     KPGHPNLVGPGKRPFQTIIPGFLTKEGKPVMSFGVMGGQMQPQGHTQMVTRVVDYQQN
                     PQAASDGPRWIVNHDFSVSFETERFAPGVLDELKRRGHRIVDPEAAMFGFGGAQLIWS
                     LDDGYCAGSDHRKDGGAVGF"
BASE COUNT         1050 a         2246 c         2245 g         1086 t
ORIGIN      
        1 gcgccgggcg cttcgtcccg gctggctcga cgctgccctt cgcgccgccg gcgtgcttaa
       61 ggagggcggg gccctgtgaa cgcaaccgcg aagcccgcga ttctcgtcac cgccgcagac
      121 ctcgcgcccc aggcgcttgc cctcctcggc gacttcgaca tcgtgtttgc gggcaggaca
      181 cccgacgagg acgccatcgc gagcctctgc gcgaagcacc agcccgtggc gatcatcgtg
      241 cgttacggga agattccggc ccgcgtgatg gacgcgtcgg cgcggcttcg cgtgatctcg
      301 aagcacggct cgggcaccga tacgatcgac aaggtggccg ccgcgcagcg cggcatcgaa
      361 gtgaaggcgg ccgcgggtgc caacgccgac gcggtggccg agcactcgtg ggcactcatc
      421 ctggcgtgcg ccaagggggt cgtcaccctc gatggccgca tgcgcgcggg cttctgggac
      481 aaggcaacgc acaagagctt cgagctcaag ggaaagaccc tcggcgtggt ggggctcggc
      541 gccatcggca ggcgtgtggc gcagatcggc ctggccttcg ggatgcgggt ggtcggccac
      601 gacccgttcg tcaccgaggc tccggcaggc gtcgcggctg cgacgctgga cgagttattc
      661 gcggcgagtc acgtggtgtc gctcaactgc ccgctcacgg atgaaaaccg gcagatggtg
      721 aatcggacgt cgatcgcgaa gatgcgcgac ggggcgatcc tggtgaatac gggccgtggc
      781 ggcctcgtgg acgaacaggc cttgctcgag gcgcttgcct cgggaaagct ccgcgcagca
      841 ggactggatg cgtattcgac ggagccgctc gtcggcgagc accctttccg cgctgtcccg
      901 aacgtggtcc tttctcccca cgtcggcggg gtgagcgagg acgcctacgt gaacatggga
      961 actgccgcag cgcgcaacgt gctcgacgcg ctttccggcg ccccccgcca ggcggcctga
     1021 tcgcgggcgc ggcggaaggc ggttgagcgg gatcaagcga gcccgccttc gtcatggaaa
     1081 ttcgcgctgg aagccggtat acactcttca ccgagagccc gacaacgggt ccgacctatg
     1141 gaggagttcc agcgtgaaaa gacgtgaatt tctggtgttg ggtggggcga tggccgcctc
     1201 gatcgcattg ccggcccgcg cggcctatcc cgagaagccg gtgcgcatga tcatcgcctt
     1261 ttccccgggc ggcgagtccg acatcggcgc gcgcctgcag cagggtgtct tccgcaagct
     1321 ctacggccag gaaatggtga tcgagtccaa gcccggcgcc ggcggggcgc tggcgtggag
     1381 cacgctcaat acctatccgg gcgacggcta cacggtgatg ggcacgaacc tgccgcacat
     1441 cgtgctgcag ccgctcgaag gcaacgtgca gtacaggacg gatgacgtca ccaacgtcca
     1501 tttctaccag tacacgcccg acgcgatcat cgtcaggaac gaaagcccgt tcaagaccta
     1561 ccaggacctc ctcaaggcgg cgaaggaagg cggcgacaag gtcaccttcg cgggttcggg
     1621 aaccaattcc gccaatcacc ttgcgcacac gcgcctcaac aagctgatgg ggacgaagac
     1681 cacgtacgtt ccgttcaagg gcaccggcga cctcctctcc tcggtcctgg gcgggcacgt
     1741 gtcggccgcg atgtcctaca acacgctcgc catctcgcag aagaacaaga tgcgcatgct
     1801 ggcggtggcc tcggagaggc ggctggcggg attcccggac gtgccgacat tcaaggaact
     1861 gggcggggac tgggtcgatg gcgcctaccg cggtgtcggc gtgccgaaat cgacgccgga
     1921 ggacctgcgc aggcgcatct ccgagatgtt ttccgagatc aacagggatg cggagttccg
     1981 ccagaagatg gccgatggcg gcttcgaact ggtcgacatc acctacgaca agatggccgc
     2041 gttcgtggcc gagcggacga aggtatatct cgagtcggcc aagctgatgg ggctcgtcaa
     2101 gtagcggcat cggcattccg gcaggagcgc cggcccgcga tgccgcatta cgggcccgca
     2161 aacgcggggc tatactgcgt gcggattcgg ggtcggcgcg aagcaggacc catcgcaaaa
     2221 catcgaggag gcgccggccc accggtcggc gacaacagcg catgaagcac ctcatcaaga
     2281 acccccgcga tttctgggcg ggcgtcatgt tcctggctct cggcgtcggc tttgccggca
     2341 tcgccttcac ctacaagctc gggaccgcgg cccgcatggg ccccgggttc ttcccgttct
     2401 tcctcgggat catcgtcgcg gcgttgggag tcggcatcct catcgctgcc atgaacccga
     2461 agaacgcggg ccagcacgtg gacaagttcc actgggggcc gatcttctgg gtgctcatgc
     2521 cgatcgtcgt cttcggcatg ttgctcaaga tcctgggcat gatgatcatg ggcctgttcg
     2581 tcgtcatcct gtcgagcatc gggagcgagg agttcaagct gcggcccacc gtgtacctcg
     2641 cgttcgggct ggtcatcttc tgcagcgccg tgttcgtggg cggcctgaag ctcccgatcc
     2701 cgctgtgccc gagcttcgag ttcttcgacc agttcgcgct ctgccgcgtc tgaggcgaac
     2761 atggaactcc tcgcgaacct cgaactcggc ttcggcgtcg cccttgcggg caccaacatc
     2821 ctctactgcc tcatcggcgt gatgctcggc acgctcatcg gcgtgctgcc cggcatcggg
     2881 cccgtggcca ccatcgccat gctgctgccg gcgaccttcg tgctgccgcc ggtatcggcc
     2941 ctcatcatgc tggccggcat ctactacggc gcgagctacg gcggctccac caccgccatc
     3001 ctcgtgaacc tgccgggaga ggtcagttcg gtggtgacct gcctggacgg ctaccagatg
     3061 gcgcgccagg gacacgcggg caaggcgctc gccatcgcgg cgatcggctc gttcttcgcc
     3121 ggcaccgtcg cgacggtcct cgtggccgcg ttcgccccgc cgctcgcgga gctcgccctc
     3181 aagttcggcc ccgcggacta cttctcgctc atggtgctgg ggctcatcgc ggcagtggtg
     3241 ctggcgcacg gctcgctcat caaggcggtg gccatgatcg tgctggggct cctcatcggc
     3301 ctcatcggca ccgacgtgaa ctccggcgtg gcccgctaca gcttcggcct gccggaactc
     3361 atcgacggcg tgggcatcgg ccccatcgcc atgggcgtgt tcggcttcgg cgagatcatc
     3421 cgcaacctcg agcatggcca gaagcgcgac gtcttcgtga agcgcgtgac cgggctgatg
     3481 ccgagctgga tcgacctcaa gatgtccttc tggccgatcg tccggggaac ggcgctgggc
     3541 tccgcgctcg gcatcctgcc cggcggcggc ccgatcctcg gctccttctc cgcctacacg
     3601 ctcgagaaga agctcgccaa ggacccgtcg cgcttcggca agggtgcgat cgagggcgtg
     3661 gccgcgccgg agtccgcgaa caacgcggcg gcgcagacgt ccttcatccc gctcctcacg
     3721 ctgggcatcc cgtccaaccc ggtgatggcg ctcatgatcg gcgcgatgat catccacggc
     3781 atccagccgg gcccgcagat catgaccgcg aacccgcagc tcttctgggg aatgatcgcg
     3841 agcatgtggg tgggcaacct cctgctcgtg gtgctgaacc tgccgatgat cggcatgtgg
     3901 gtgaagctcc tcacgctgga ataccgcttc ctgtacccgg cgatcctgct cttctgctgc
     3961 atcggcgtgt actcgatcaa caacacctgg atggacgtga tcctcgccgc cggcttcggc
     4021 ttcatcggct acctgttcgg aaagctcgac tgcgaggccg cgcccctgct gctgggcttc
     4081 gtgctggggc cgatgatgga ggagaacctg cgccgtgcgc tgctgctttc gcgcggcgat
     4141 ccggccgtct tcgtgacgcg gcccatcagc gcatcgctcc tcgtggtggc ggcgctcctg
     4201 ctcgtggtcg tcctcgcgcc ctcggtgaga aagaagcgcg aggagacgtt cgtcgaggaa
     4261 gtctaggcgg cgagcagccg cgccagcccg gcgaggtctt ccacgaccgc gtcgggcggc
     4321 gagcccagcc tgtcgacggg cgccttcccg cggttcaccc agaaggcggt gaagccgaag
     4381 gaccgggccc ccgcggcgtc ccatccgttc gaggacacga aaccgatcgc atcggcgggc
     4441 agctcgaggc cggcagtggc gatcgcatag acccgcgggt cgggcttgta gacgccggct
     4501 tcgtgcacgc tgaagacacg ctcgccgaag aagatgcgca gctcgttgtg gtcgaccacg
     4561 gcgtcgagca tgtcgggatg cccgttggag aggatggcga gagggcggcg ctcgcccagc
     4621 cgctcgagcg cgccgcgcac ctccgggaac gtgcccagcg tgcggtaggc gagcaggagc
     4681 gccgtgcggt ccccggctcg cgcggcgaga ccgagcgatt ccagggccca gtcgagcgcc
     4741 ccttcggtga ccgcgccgaa gtcgtcgtag cggcccatga ggctgcgcag ccatgtgtac
     4801 tcgagctgct tcgagcgcca cgcctgcgac agggctgccc cgcgcccggg aaagagcgag
     4861 tcggccaggc gcgtgacgga gtgcacgtcg aaaagcgtgc cgtaggcgtc gaagacgagg
     4921 gcttgcgggg cgtgcatggc ggttcctcct cggcgcgaga atacgccgca cgggttggcg
     4981 gtgctaccat tcgggctacc ccgacccgcc gcctcctgac catgttcacc tgggatttcc
     5041 cgtatccgtc gcagcgcatg ccgctgctcg ccaagaacgt cgtcgccacc tcgcagccgc
     5101 tggctgccca ggcgggcctg caagcgctcg cgaaaggcgg caacgcggtg gacgccgcgc
     5161 tcgccgcggc gatcacgctc accgtcgtcg agcccacttc caacggcatc ggctcggacg
     5221 ccttcgcgat cctctgggac ggcgagaagc tcgtgggcct caacgcctcg ggccgtgcgc
     5281 cggccggctg gacgccggcg cgattcaagg gtcgcgaggc gatgccggtg cacggctggg
     5341 acgccgtgac ggtgccgggc tgcgtctcgg cgtgggtcgc gctgtcgcag cgctacggaa
     5401 agctccattt ttccgatctc ttccaggctg cgatccgata tgcgcgcgac ggctacatgg
     5461 tctcgcccat caccgccgca aactgggcgc ggcaggcgcc caacttcacc ggcttctccg
     5521 agttcgcgtg gaccttcctg cccaaggacc gcgcgccgcg cgccggcgag cgcttctatt
     5581 gcccgcagca ggccgagacg ctcgaggaga tcgcggccac gaagggcgag agcttctacc
     5641 ggggcaagct cgccgagcgc attgcgctcg cctcccaggc ggacgagggc gcgcacacgc
     5701 tcgccgattt cgcggcgcac cgctgcgact gggtcgagcc gctgtcggtc gaataccgcg
     5761 gctaccggtt gcacgagatt ccgcccaatg ggcaggggat cgtggccctc atggcgctcg
     5821 gcatcctgcg ccagttcgac cttgccgccc tgcccgtgga tcgcgccgac agcctgcacc
     5881 tgcagctgga agcgatgaag ctcgccttcg ccgacgcgta ccgctacgtc tcggacccgg
     5941 ccacgatgga gttcgacgcg aagatgctgc ttgccgacga ctacctggcc gggcgggccc
     6001 gggcgatcga catgaagcgc gcccaggacc cgccgccggg cgagccgcgc gccggtggca
     6061 cgatctacct cacgacggcg gacgccagcg gcatgatggt gtcgtacatc cagtcgaact
     6121 acatgggctt cggttccggc atggtggtgc ccggcacggg gatctcgatg cagaaccgcg
     6181 gcgcgggctt ctcgctcaag cccggccatc ccaacctcgt cggtcccgga aagcgcccct
     6241 tccagacgat catcccggga ttcctcacca aggagggcaa gccggttatg agcttcggcg
     6301 tgatgggcgg gcagatgcag ccccagggcc acacgcagat ggtcacacgc gtcgtcgact
     6361 accagcagaa tccgcaggcg gcgagcgacg ggccgcgctg gatcgtcaac cacgacttca
     6421 gcgtgagctt cgagaccgag cgcttcgcgc ccggcgtgct ggatgagctg aagcgtcgcg
     6481 gccaccgcat cgtggacccc gaggccgcga tgttcggctt cggcggcgcg caactcatct
     6541 ggagcctcga cgacggctat tgcgccggtt cggaccaccg caaggacggc ggcgcggtcg
     6601 gcttctgacg aaaaggggtc aggttac
//
LOCUS       DASUEV010000034        12709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_124753, whole genome shotgun sequence.
ACCESSION   DASUEV010000034 DASUEV010000000
VERSION     DASUEV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12709
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124753"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="prfA"
                     /locus_tag="VFV55_01015"
     CDS             complement(<1..893)
                     /gene="prfA"
                     /locus_tag="VFV55_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX4942898.1"
                     /translation="MKPSVAAKLASLVSRAKELDALLSDPDVTANLDNYRKLTREHAE
                     IAPVVGRYGEYVKAQADIAAAEEMAGDPQMRDFAEAEIRSGREKLDAIAADIQAMLLP
                     TDPNDERNLFLEIRAGTGGDESALFAGDLFRMYARFAERNRWQVEVISESPGDAGGCK
                     EVIARIVGQGAYSRLKFESGGHRVQRVPATETQGRIHTSAATVAVMPEADEAGEIEIN
                     PAEVRVDTYRSSGAGGQHVNKTDSAVRLTHLPTGIVVECQEGRSQHKNREQAWRILAA
                     RIKAKRDSEQQAREASERKSLV"
     gene            complement(890..2164)
                     /gene="hemA"
                     /locus_tag="VFV55_01020"
     CDS             complement(890..2164)
                     /gene="hemA"
                     /locus_tag="VFV55_01020"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX4942899.1"
                     /translation="MPLLVLGINHHSAPLEVREKLAFPPETQADALTSLAGRPGVAEA
                     VLVSTCNRTEIYCRADDMAPVRAWLKEQAARSGLDLEGLLYAHSEAEAIRHAFRVASG
                     LDSMVLGEPQILGQVKQSVRTAVHSGTLGSTLNRLFQHTFSVAKEVRTQTAIGAQSIS
                     MSAAALKLAQNLFGDISRTKLLLIGVGEMVELAATYFVAQGPQSVVVANRTLARGEAF
                     AERFSGTAISLEQLAERLHEFDVVITGTASTLPILSKSAFERALKARRYRPMFVADFA
                     VPRDVEPEASELEDLFLYTIDDLGTIVQEGAGQRQAAVAEAEIIIDRQVDAFRAWLSA
                     RGAVPAIVQLRAKADEYRAAELAKAKSRLARGEDPAKVLEGLASGLVNKILHHPTQAL
                     NRAAERERDSLVRAVEILFPDDGNGREEPGEQ"
     gene            2323..2859
                     /locus_tag="VFV55_01025"
     CDS             2323..2859
                     /locus_tag="VFV55_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX4942900.1"
                     /translation="MSRTLAAALLWVALPAAAACPALLDHRMATIKGEPTDLCQYAGK
                     VVLVVNTASYCGYTGQYEGLEALHQRYRDRGLVVLGVPSNDFGAQEPGSNEQVADFCE
                     RTYKVRFPMLAKAVVSGPGAIALYRELAGKTGKAPGWNFHKYLLGRDGQAIASFPSAV
                     PPGDARLVAAVEKALAVR"
     gene            2954..4660
                     /gene="pilB"
                     /locus_tag="VFV55_01030"
     CDS             2954..4660
                     /gene="pilB"
                     /locus_tag="VFV55_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX4942901.1"
                     /translation="MSAAPGTYSALGRALVQQGRLVQADATAIQLEASKAGVTFVQQL
                     VSSKKLSARDISQFAAHAFGYPLLDLSAVDTDQLPLNLIDSKIVTNHRVIALGKRGNR
                     LFVGMSDPTNEQVIQEVKFATGMTMEPVVVEDDKLTIAVKKLGDATIRAFEVGTEEVD
                     LSGVEVTESDAQPEVVTEDIDDAPVVRYVQKILLDAINGGASDIHFEPYEKFYRIRYR
                     QDGVLYDVAQPPLAIKEKIASRIKVVSRMDISEKRVPQDGRMKIVLSKTRAIDFRVST
                     LPTLFGEKICIRILDSSNTMLGIDALGYEPDQREALMAAIQRPYGMVLVTGPTGSGKT
                     VSLYTCLNILNKPGINISTAEDPAEIHLPGINQVNMNDKAGLTFAVALKAFLRQDPDV
                     IMVGEIRDLETGEIAIKAAQTGHMVLSTVHTNDAPATLSRLMNMGIAPFNIASSVILI
                     TAQRLARKLCTCKRPVSIPEEALLRAGFREEDLNGTWQTYEPVGCEVCKNTGYKGRVG
                     IYQVMPVSEEIERIIMKNGTSIDVGDQAQRDGVRDLRQSGLLKVRRGLTSLEEIEAVT
                     NE"
     gene            4672..5898
                     /locus_tag="VFV55_01035"
     CDS             4672..5898
                     /locus_tag="VFV55_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4942902.1"
                     /translation="MAAVAKKDAKDVKLNNYSWSGKDKNGKVVKGEVRAGGENVVMAT
                     LRRQGIQVSSVKKVSRRGGSKITDKDITIFTRQLAVMMKAGVPLLQAFDIVGKGHSNP
                     SVGKLLLDIKTEVETGSSLSAAFRKYPQHFDALFCNLVGAGEQAGILDSLLERLATYK
                     EKILAIKGKIKSALFYPVSIIVVAIVITAVIMIFVIPTFKDLFKGFGADLPMPTQVVM
                     AMSDFFVAYWYIIFGVMFGGAWFLNYTYKRSTKMQNGMDRLILRIPVFGEVVRKASIA
                     RWCRTLSTMFAAGVPLVESLDSVAGAAGNYVYYEATKRIQAEVSSGTGLTVAMQNTNV
                     FPSMVLQMTAIGEESGALDAMLAKVADFFEAEVDDAVDALASLMEPLIMVVLGGLIGG
                     MVVAMYLPIFKMGQAI"
     gene            5968..6831
                     /locus_tag="VFV55_01040"
     CDS             5968..6831
                     /locus_tag="VFV55_01040"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX4942903.1"
                     /translation="MDFSPLQSSPALLALAAGILGLAVGSFLNVVIHRLPIMMQAEWR
                     TDCAEFEGREPPPAAQLNLVTPRSRCPSCGTPITAMQNIPLASWVALGGKCAACKAPI
                     SVRYPLVELFTGLASAFLAWRFGWGPALGAALVFTWALIAASAIDIDTQLLPDRITLP
                     LLWLGLLVNVTGLFVDLRSAVLGAAGGYLLLWSVYWGFRLLAGKEGMGFGDFKLLAAL
                     GAWTGWQTLPLILLVSAGAGAVIGIVVIWLSGKGRDTRIPFGPYLAAGGLVALLWGHG
                     AVVAYLGRFPT"
     gene            6828..7433
                     /gene="coaE"
                     /locus_tag="VFV55_01045"
     CDS             6828..7433
                     /gene="coaE"
                     /locus_tag="VFV55_01045"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409832.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX4942904.1"
                     /translation="MKRTVGLTGGIGSGKSTAAGFLAELGATVVDADDISRELTAAGG
                     AALPALREAFGERFVPAGGALDRAAMRALAFSDSAVRARLEGILHPLIRAESKRRLAA
                     ATGPYAVLVVPLLFESGSWTGQLDRVVVVDLDGELQVRRAAERSRLSPDEVRAIMAAQ
                     WPRWRRLQAADEVLWNGGDPAALRAQCERLHARLCGAGANA"
     gene            7522..8277
                     /gene="zapD"
                     /locus_tag="VFV55_01050"
     CDS             7522..8277
                     /gene="zapD"
                     /locus_tag="VFV55_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX4942905.1"
                     /translation="MITYEYPLNERVRTLLRLEDLYDRVQFFLEGDSPHDHHACLTGI
                     FEILEVASRADLKSDLLQELDRQRTFLDALHANPAISEEKLDEILAAIDRAFTNLHGL
                     SGKTGQNLRENEWLMTIKQRAGIPGGTSEFDLPSYHYWMHRPVDARRADLVSWIRPID
                     AVHAALSIVLRVLRESGRNVSLVATQGVFQQGPGDKPAQLLRLSLPEALACVPEISAN
                     KYALNIRFLVPEGVQKSRVFEHDVAFDLSFCNL"
     gene            8274..8513
                     /locus_tag="VFV55_01055"
     CDS             8274..8513
                     /locus_tag="VFV55_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX4942906.1"
                     /translation="MSAGTRRPERLVACPGCAKLVAYSPANPWRPFCSERCRQTDLGA
                     WAAGAYRIPLRGDDPESPRDEGDEDGASGHATSSK"
     gene            8732..9928
                     /gene="coxB"
                     /locus_tag="VFV55_01060"
     CDS             8732..9928
                     /gene="coxB"
                     /locus_tag="VFV55_01060"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX4942907.1"
                     /translation="MLRTTLRNAALAAAALAVAASANAAYDVDILPPASPIARQIYDL
                     HWAILWVCVVIFIVVFGTMFYSVFKHRKSVGAKAANFHENTTVELIWTVIPFIILIGM
                     AYPATKTVLEMKDASSPDLTIKVTAYQWAWEYDYLQDGVKFNSLLATPRAQIEEWQRK
                     GDKKNADYLLEVDNPMVVPAGKKVRLLVTANDVIHGWYVPQLGINQYGIPGFVKDTWF
                     KAEKPGTYRGQCSQICGKEHGYMPIVVVAKAPDEYAAWVKEQKAKMPPAGAAAPQVAP
                     VAASAPASSAPAAAQDIGKKWTLDELKAAGEKVFAANCVACHQVTGKGLPPAFPPLDG
                     SKVVNGPKAAQLSLVLNGKQGTAMASFARLSDSELASVITYTRNSWGNRTGEAIQPSE
                     VSAARK"
     gene            9965..11560
                     /gene="ctaD"
                     /locus_tag="VFV55_01065"
     CDS             9965..11560
                     /gene="ctaD"
                     /locus_tag="VFV55_01065"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX4942908.1"
                     /translation="MSAAAPAHDHVHHDDHAHGHPSGLRRWLFTTNHKDIGTLYLWFS
                     FVMFLTGGVMALLIRAELFQPGLQFLNPEFFNSLTTVHGLVMVFGAIMPAWVGFANWL
                     IPMQIGAADMAFPRMNNWSFWLVVPAAILLVASFFVPGGAAASGWTLYPPLIIQQGIS
                     IDMTILAVHILGASSIMGSINIITTILNLRAPGMTLMRMPLFVWTWLITAYLLIAVMP
                     VLAGSVTMLLTDRHFGTSFFNAAGGGDPTMFMHIFWFFGHPEVYIMILPAFGVISAII
                     PAFARKPLFGYASMVYATASIAILSFIVWGHHMFTVGMPAAGNLFFMYATMLIAVPTG
                     VKIFNWIATMWRGSMTFETPMLFSIGFLCLFTIGGFSGLVLAITPVDITLHNTYYVVA
                     HFHYVLVSGALFAIFAGVYFWLPKWTGNMYHEGLGKLHFWLSVIGFNIAFFPQHFLGL
                     AGMPRRIPDYALQFAEWNMWSSIGAYIFGFSQLLFLYVVIECIRSGRKSEQRPWEGAD
                     SLEWTHLPCPAPYHTFETAPVLK"
     gene            11691..12221
                     /locus_tag="VFV55_01070"
     CDS             11691..12221
                     /locus_tag="VFV55_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX4942909.1"
                     /translation="MTDAVLAARNRQMMGKLLVIAVAMLGFGFAMVPMYRQICESLGI
                     SQGRVVGATNTQVDASRSVTVELLASSAGLPWRFESLEREVKLHPGQLVTVNYRVVNT
                     LDRPVTAHAVMNAAPADAGQYIEKQACFCFSTQTLAAGEERVMPVVFLVSPRAPRDMT
                     TISLSYTFFEQPGSAS"
     gene            12218..12391
                     /locus_tag="VFV55_01075"
     CDS             12218..12391
                     /locus_tag="VFV55_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEX4942910.1"
                     /translation="MMDAFKAVFWSFFGIRRNKEYEADAKRLTPQAVIAAGIVTALVV
                     VLAIFGLVKLVTR"
     gene            12435..>12709
                     /locus_tag="VFV55_01080"
     CDS             12435..>12709
                     /locus_tag="VFV55_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX4942911.1"
                     /translation="MGNAQPGGYYYAPPSHWPIVGSAALLLMAIGGVLLMNSIGGGGI
                     VFGIGAAVLVYMLFGWFGTVIRESEGGLFNKQVDMSFRWSMSWFIFS"
BASE COUNT         2016 a         4313 c         4242 g         2138 t
ORIGIN      
        1 acgaggcttt tcctctcgct cgcctcccgg gcctgctgct cgctgtcgcg cttcgccttg
       61 atgcgcgcgg caaggatgcg ccaggcctgc tcgcgattct tgtgctggct ccttccttcc
      121 tggcactcga ccacgatgcc cgtgggcagg tgcgtcaggc gcactgcgga atcggtcttg
      181 ttcacgtgct gcccgcccgc gccgctggag cggtaggtgt cgacgcgcac ctcggcgggg
      241 ttgatctcga tctcgcccgc ctcgtcggcc tcgggcatca cggcgacggt ggcggcggag
      301 gtatggatgc ggccctgtgt ctcggtggcc ggcacgcgct gcacgcgatg gccgccggac
      361 tcgaacttga gcctcgagta ggcgccctgg ccgacgatgc gcgcgatgac ctccttgcag
      421 ccgccggcat cgccggggct ttcggagatg acctcgacct gccagcggtt cctctccgcg
      481 aaccgggcgt acatgcggaa gaggtcgccg gcgaagagcg cgctctcgtc gccgccggtg
      541 ccggcccgga tctcgaggaa gaggttcctc tcgtcgttgg ggtccgtcgg caggagcatc
      601 gcctggatgt cggcggcgat tgcgtcgagc ttttcgcggc cggacctgat ctcggcctcg
      661 gcgaagtcgc gcatctgcgg atccccggcc atctcctcgg ccgccgcgat gtcggcctgc
      721 gccttgacgt actcgccgta gcgccccacg acgggcgcga tctcggcgtg ctcccgggtg
      781 agcttgcggt agttgtcgag attcgccgtg acatcggggt cggacaggag cgcgtccagc
      841 tccttcgcgc gcgacaccag gctcgcgagt ttcgcggcca cgctcggctt cattgctccc
      901 ccggctcttc ccggccgttg ccgtcgtcgg ggaagaggat ttcgaccgcg cgcacgaggg
      961 agtcgcgctc gcgctcggcg gcgcggttca gggcctgcgt cgggtggtgc aggatcttgt
     1021 tcaccagccc gctcgcgagc ccttcgagga ctttcgcggg atcctcgccc cgcgcgagcc
     1081 ggctcttcgc cttggcgagc tcggcggcgc ggtattcgtc ggccttggcg cgaagctgga
     1141 cgatggcggg cacggcgccg cgggcggaaa gccaggcgcg gaacgcgtcc acctgccggt
     1201 cgatgatgat ctcggcctcc gccaccgcgg cctgccgctg cccggcacct tcctggacga
     1261 tggtgccgag gtcgtcgatg gtgtagagga agaggtcctc gagctcggat gcctccggct
     1321 ccacgtcgcg cggcaccgcg aaatccgcca cgaacatcgg gcggtagcgc cgcgccttga
     1381 gcgcgcgctc gaaggcggac ttggacagga tgggcagcgt cgaggcggtg ccggtgatga
     1441 cgacgtcgaa ctcgtgcagc cgctcggcga gctgctcgag cgagatggcc gtcccgctga
     1501 agcgctccgc gaaggcctcc ccgcgcgcga gcgtccggtt ggccacgacg accgactgcg
     1561 gcccctgcgc gacgaaatac gtggcggcga gctccaccat ctcgcccacg ccgatgagca
     1621 gcagcttggt gcgcgagatg tcgccgaaga ggttctgcgc gagcttgagc gccgctgcgg
     1681 acatggagat cgactgcgcc ccgatcgccg tttgggtgcg cacttccttg gccaccgaga
     1741 aggtgtgctg gaagaggcgg ttgagcgtcg agcccagcgt gccgctgtgc accgcggtgc
     1801 gcacagactg cttcacctgc ccgaggatct gcggctcgcc cagcaccatc gaatccagcc
     1861 cggaagccac gcggaaggcg tgccggatgg cctcggcctc gctgtgcgcg tagagcaggc
     1921 cttcgaggtc gagacccgac cgggcggcct gttccttgag ccaggcgcga accggcgcca
     1981 tgtcgtcggc gcggcagtag atctcggtgc ggttgcaggt ggagacgaga acggcctccg
     2041 ccacgcccgg ccggccggcg agcgacgtga gcgcgtcggc ctgggtctcg ggggggaagg
     2101 cgagcttctc ccggacctcg agcggcgcgc tgtggtggtt gatgccaagg acgagcaggg
     2161 gcatgggccg tgatgcctcg ccgtgcgacg gcgggcaatt cgagagcgcg gattatacag
     2221 ggggggccac ccgggcccct tgcgggattc gggatggcgt cgggaggccc gcggtctggg
     2281 tatcatgggg tggcacgcgt tgaccatgga aaggacgtca agatgagccg aaccctcgcc
     2341 gccgccctcc tgtgggttgc gcttcccgcc gcggccgcct gcccggcgct cctcgaccac
     2401 cgcatggcga cgatcaaggg cgagcccacc gacctttgcc agtacgccgg caaggtggtg
     2461 ctggtggtca ataccgcgag ctactgcggc tacacgggcc agtacgaagg gctcgaagcg
     2521 ctccaccagc gctaccggga ccgcgggctg gtggtgctgg gcgtaccctc gaacgatttc
     2581 ggcgcccagg agccgggctc caacgagcag gtggccgact tctgcgagcg cacctacaag
     2641 gtccgctttc cgatgctcgc gaaggcggtc gtctccggtc cgggcgcgat tgcgctctac
     2701 cgcgaactgg ccgggaagac cggcaaggcg ccgggctgga atttccacaa gtacctgctc
     2761 gggcgcgacg ggcaggccat cgccagcttt ccgagcgccg tcccgcccgg ggacgcgcgg
     2821 ctggtggccg ccgtggagaa ggcgcttgcc gttcgctgac cccggattcc cgtggccgaa
     2881 cgcatcccgg accattcttt acaagaagaa cgaaaaaggc gcactattac tttgatttaa
     2941 aggaatcttt ttaatgtccg ctgcccccgg tacgtacagc gccctcggtc gcgccctggt
     3001 ccagcagggt cgtctcgtgc aggccgacgc cacggcgatc cagctcgagg cgtcaaaggc
     3061 cggggtgacg ttcgtgcagc agctcgtctc ctcgaagaaa ctctcggcgc gggacatctc
     3121 gcaattcgcg gcccacgcgt tcggctaccc gctgctcgac ctttcggccg tggataccga
     3181 ccagcttccg ctcaacctca tcgacagcaa gatcgtcacc aaccaccggg tgatcgcgct
     3241 gggcaagcgc ggcaaccgcc tcttcgtcgg catgtccgac ccgaccaacg agcaggtgat
     3301 ccaggaggtg aagttcgcca ccggcatgac catggagccg gtggtggtcg aggacgacaa
     3361 gctcacgatc gcggtgaaga agctgggcga tgccacgatc cgggcgttcg aggtcggcac
     3421 cgaggaagtg gacctctcgg gcgtcgaggt gaccgaaagc gacgcgcagc ccgaggtcgt
     3481 caccgaggat atcgacgacg ccccggtggt gcggtacgtg cagaagatcc tcctcgacgc
     3541 catcaacggc ggcgcatccg acatccactt cgagccctac gagaagttct accgcatccg
     3601 ctaccggcag gacggcgtgc tctacgacgt ggcgcagccg ccgctcgcga tcaaggagaa
     3661 gatcgcctcg cgcatcaagg tggtctcccg gatggacatc tcggagaagc gcgtgccgca
     3721 ggacgggcgc atgaagatcg tgctttccaa gacgcgcgcc atcgacttcc gcgtgtccac
     3781 cctgccgacg ctcttcggcg agaagatctg catccgcatc ctcgattcct cgaacaccat
     3841 gctcgggatc gacgcgctgg gctacgagcc ggaccagcgg gaggcgctga tggccgcgat
     3901 ccagcgcccc tacggcatgg tgctggtcac cgggccgacg ggttccggca agacggtgtc
     3961 gctctacacc tgcctcaaca tcctcaacaa gcccggcatc aacatctcga ccgcggagga
     4021 ccccgccgaa atccacctgc cgggcatcaa ccaggtgaac atgaacgaca aggcgggcct
     4081 cacgttcgcg gtggcgctca aggcgttcct gcgccaggac ccggacgtca tcatggtggg
     4141 cgagatccgc gacctcgaga cgggcgagat cgccatcaag gcggcgcaga cgggccacat
     4201 ggtgctctcg accgtgcaca cgaacgacgc gcccgcgacc ctgtcgcgcc tcatgaacat
     4261 gggcatcgcg ccgttcaaca tcgcgtcctc cgtgatcctc atcaccgcgc agcgcctggc
     4321 gcgcaagctc tgcacctgca agcggccggt gtcgattccc gaggaggcgc tcctgcgcgc
     4381 cggtttcagg gaggaggacc tgaacggaac ctggcagacc tacgagcccg tcgggtgcga
     4441 ggtgtgcaag aataccggct acaagggccg cgtcggcata taccaggtca tgccggtctc
     4501 cgaggagatc gagcgcatca tcatgaagaa cgggacgtcg atcgacgtcg gcgaccaggc
     4561 gcagcgcgac ggcgtgcgcg acctgcgcca gtcggggctt ctcaaggtac gcaggggact
     4621 cacctcgctc gaagagatcg aggccgtcac gaacgagtag ggagaacggg catggcagcg
     4681 gtggccaaga aggatgcgaa ggacgtcaag ctcaacaact attcgtggag cggcaaggac
     4741 aagaacggca aggtcgtgaa gggcgaggtg cgcgcgggcg gcgagaacgt cgtgatggcc
     4801 acgctgcggc gccagggcat ccaggtgtcg agcgtgaaga aggtgtcgcg gcgcggcggc
     4861 tccaagatca cggacaagga catcacgatc ttcacgcgcc agctcgcggt gatgatgaag
     4921 gccggcgtgc cgctgctgca ggccttcgac atcgtgggca agggccacag caacccgtcg
     4981 gtgggcaagc tcctcctcga catcaagacc gaggtcgaga ccgggtcctc gctctccgcc
     5041 gcgttccgca agtacccgca gcacttcgac gcgctcttct gcaacctggt cggcgccggc
     5101 gagcaggcgg gcatcctgga cagcctgctc gagcgcctgg cgacctacaa ggaaaagatc
     5161 ctcgccatca agggcaagat caagagcgcc ctcttctacc cggtgtcgat catcgtggtg
     5221 gccatcgtga tcacggcggt gatcatgatt ttcgtgatcc cgacgttcaa ggacctgttc
     5281 aagggcttcg gggcggacct tcccatgccc acgcaggtcg tgatggcgat gtccgacttc
     5341 ttcgtcgcct actggtacat catcttcggc gtgatgttcg gcggcgcctg gttcttgaac
     5401 tacacctaca agcgctccac caagatgcag aacggcatgg accggctgat actgcgcatc
     5461 ccggtgttcg gcgaggtggt gcgcaaggcc tccatcgcgc gctggtgccg cacgctctcg
     5521 acgatgttcg ccgcgggcgt gccgctcgtg gagtcgctcg actccgtggc gggcgcggcc
     5581 ggcaactacg tctactacga ggcgaccaag cgcatccagg cggaggtctc gtcgggcacg
     5641 gggctcacgg tggcgatgca gaacaccaac gtcttcccga gcatggtgct gcagatgacg
     5701 gcgatcggcg aggaatccgg ggcgctggat gcgatgctcg ccaaggtggc cgacttcttc
     5761 gaggccgagg tggacgatgc cgtggacgcg ctcgcgtccc tcatggagcc cctcatcatg
     5821 gtggtcctgg gcggcctcat cggcggcatg gtggtggcca tgtaccttcc gatcttcaag
     5881 atgggccagg cgatctgacg cgaagcgcag cgatgcgacg ggcgcgggag gcgatttccg
     5941 cgcccgcttt catttgagcc gacgacgatg gacttctccc cgctgcagtc ctcacccgcg
     6001 ctcctcgcgc tcgccgccgg gatcctcggg ctcgccgtgg gctccttcct gaacgtggtg
     6061 atccaccggc tgccgatcat gatgcaggcg gagtggcgca cggactgcgc ggagttcgag
     6121 gggcgggagc cgccgcccgc ggcgcagctc aacctggtca cgccgcggtc gcgctgcccg
     6181 tcgtgcggca cgccgatcac cgcgatgcag aacattccgc tcgcgagctg ggtggccctg
     6241 ggcggcaagt gcgcggcgtg caaggcgccc atcagcgtgc gctaccccct ggtggagctg
     6301 ttcacgggcc tggcgtcggc gtttctcgcc tggcgcttcg gctggggccc ggcgctcggc
     6361 gcggcgctcg tcttcacctg ggcgctcatc gcggcgtccg cgatcgacat cgacacccag
     6421 ctcctgcccg accgcatcac gctcccgctc ctgtggctgg gcctgctcgt gaacgtcacc
     6481 gggctcttcg tcgacctgcg ctccgcggtg ctgggcgcgg caggcggcta tctcctgctg
     6541 tggagcgtgt actggggatt caggctcctc gcgggcaagg aggggatggg cttcggcgac
     6601 ttcaagctcc tggctgccct cggcgcgtgg accggctggc agacgctgcc tctcatcctg
     6661 ctggtgtcag ccggggcggg cgcggtgatc ggaatcgtcg tgatctggct gtcgggcaag
     6721 gggcgcgaca cgcgcatccc gttcggcccc tacctcgcgg cgggcgggct ggtggcgctg
     6781 ctctgggggc acggggcagt cgtcgcctac ctcggtcggt tccccacttg aagcgcaccg
     6841 tcggcctgac gggcgggatc ggctcgggaa agtccaccgc ggccgggttc ctggcggaac
     6901 ttggcgcgac cgtggtcgac gcggacgaca tctcgcgcga gctcacggcg gccggtggcg
     6961 ccgcgcttcc cgcgctgcgc gaggcgttcg gcgaaaggtt cgtgccggcc ggcggggccc
     7021 tggaccgtgc cgccatgcgc gcgctcgcct tctcggattc cgcggtgcgc gcgcggctgg
     7081 aaggcatcct gcacccgctc atccgggcgg aatcgaagcg gcggctcgcc gcggcaaccg
     7141 gcccctatgc ggttcttgtc gtgccactgc tcttcgaatc gggatcgtgg accggccaac
     7201 tcgatcgcgt cgtggtggtc gacctggacg gggaactcca ggtccggcgc gccgccgagc
     7261 gttcccggct ttcccccgac gaagtccggg cgatcatggc tgcccaatgg ccgaggtggc
     7321 ggcggctgca ggcggccgac gaggtcctgt ggaacggggg cgatcccgcg gcgctgcgcg
     7381 cacaatgcga acggctgcac gcgcggcttt gcggcgccgg cgcaaatgcg tgaggcgccc
     7441 ggatttgtca aggatgggac ggatgcccca caatcgagca aaatcgaaaa tcgtcccgac
     7501 acccaggggc tccgtgcggc cgtgatcacc tacgaatatc ccctgaacga gcgcgtgcgt
     7561 acgctgcttc gcctggagga cctctacgac cgcgtccagt tcttcctgga gggcgacagt
     7621 ccgcacgacc accacgcctg cctcaccggg atcttcgaga tcctcgaggt ggcgagccgc
     7681 gcggacctga agagcgacct gttgcaggag ctcgaccgcc agcgcacctt cctcgacgcc
     7741 ctgcatgcca acccggcgat ctcggaggag aagctcgacg agatcctcgc cgcgatcgac
     7801 cgcgcgttca cgaacctgca cggcctctcc ggcaagaccg ggcagaacct gcgcgagaac
     7861 gagtggctca tgacgatcaa gcagcgcgcc gggattccgg gcggcacgag cgagttcgac
     7921 ctgccctcct accattactg gatgcaccgg cccgtggatg cccgccgtgc cgacctcgtg
     7981 tcgtggatcc ggcccatcga cgcggtgcac gcggcgcttt ccatcgtgct gagggtgctg
     8041 cgcgaaagcg gccgcaacgt ctcgctggtg gcgacccagg gggtgttcca gcagggcccc
     8101 ggcgacaagc cggcgcagtt gttgcgcctt tcgctgccgg aagcgctggc ctgcgtgccg
     8161 gagatcagcg ccaacaagta cgcgctcaat atccgcttcc tcgttcccga aggggtgcag
     8221 aagagccggg tgttcgagca cgacgtggca ttcgaccttt ccttctgcaa cctgtgagcg
     8281 cagggacccg gcgccccgaa cggctcgtcg cgtgccccgg ctgcgcgaag ctggtcgcat
     8341 actcccccgc caacccgtgg cggcccttct gctcggagcg ctgccgccag actgaccttg
     8401 gggcgtgggc cgccggggcg taccgcatac ccctgcgcgg ggacgacccc gaatcgccgc
     8461 gggacgaggg tgacgaggac ggcgcctcgg gacacgcgac ttcgtcaaaa taaggtttct
     8521 ttgcgctagg tcaaaggctt gggctagaat ccgcggctag ccgaatcccg caccatgtcg
     8581 ctcccgccag atcggccacc ggaagtccgg gtggcgtcga accgattgct gggcccgcac
     8641 gggcgcacgg cctgcctcgc ggcagtcgcg ctcgtcgcga ctgccggtga cccggacacg
     8701 aatcgacaag actgacaaca tgggccccga catgcttcgc acaaccctcc gaaacgccgc
     8761 ccttgccgcc gccgcgctcg ccgttgccgc ctcggctaac gccgcctacg acgtcgacat
     8821 cctgccgccg gcctcgccca tcgcccggca gatctacgac ctgcactggg ccatcctctg
     8881 ggtctgcgtc gtcatcttca tcgtggtgtt cggcacgatg ttctattccg tattcaagca
     8941 ccggaagtcc gttggtgcga aagccgccaa cttccacgag aacacgacgg tcgagctgat
     9001 ctggacggtg atcccgttca tcatcctcat cggcatggcc tatcccgcca ccaagacggt
     9061 gctcgagatg aaggacgcct ccagccccga tctcaccatc aaggtgacgg cctaccaatg
     9121 ggcctgggag tacgactacc tccaggacgg ggtgaagttc aactcgcttc tcgccacgcc
     9181 tcgcgcccag atcgaggaat ggcagaggaa gggcgacaag aagaacgccg actacctgct
     9241 cgaggtggac aatcccatgg tggtccccgc gggcaagaag gttcgcctgc tcgtcaccgc
     9301 caacgacgtg atccacggct ggtacgtgcc gcagctgggc atcaaccagt acggcatccc
     9361 cggcttcgtg aaggacacct ggttcaaggc cgagaagccc ggcacctacc gcggccagtg
     9421 cagccagatc tgcggaaagg aacacggcta catgccgatc gtcgtggtgg ccaaggcccc
     9481 ggacgagtac gccgcctggg tgaaggagca gaaggcgaag atgcccccgg ccggggccgc
     9541 ggccccgcag gtggcgccgg tcgccgcatc ggcccccgct tcctccgcgc cggcagccgc
     9601 gcaggacatc ggcaagaagt ggaccctgga cgagctcaag gctgccggcg agaaagtgtt
     9661 cgccgccaac tgcgtggcct gccaccaggt cacgggcaag ggcctgccgc ccgccttccc
     9721 gccgctagac ggctcgaagg tcgtcaacgg cccgaaggcc gcccagctga gcctcgtcct
     9781 caatggcaag cagggcaccg cgatggcctc gttcgcgcga ctctccgaca gcgaactcgc
     9841 gtcggtcatc acctacacgc gcaattcctg gggcaacagg accggcgagg cgatccagcc
     9901 ttccgaggtc agtgccgccc gcaagtagcc ccacccaccg gattcgagaa agcggagtca
     9961 cgacatgagc gcagcagccc cggcccacga ccacgtccac cacgacgacc atgcccatgg
    10021 ccacccgtcg gggctcaggc gctggctgtt cacgacgaac cacaaggaca tcggcaccct
    10081 ctacctgtgg ttctcgttcg tgatgttcct caccggcggc gtgatggcgc tcctcatccg
    10141 cgccgagctc ttccagccgg ggctgcagtt cctgaacccg gagttcttca actcgctcac
    10201 cacggtgcac gggctggtga tggtgttcgg cgcgatcatg ccggcgtggg tggggttcgc
    10261 caactggctc atcccgatgc agatcggcgc ggcggacatg gccttcccgc gcatgaacaa
    10321 ctggagcttc tggctggtgg ttcccgccgc gatcctgctg gtggcctcgt tcttcgtccc
    10381 gggcggcgcc gccgcctccg gctggacgct ctatcccccg ctcatcatcc agcaggggat
    10441 ctcgatcgac atgaccatcc tggcggtgca catcctgggc gcctcgtcga tcatgggctc
    10501 gatcaacatc atcaccacca tcctgaacct gcgcgcaccg ggcatgaccc tcatgaggat
    10561 gcccctcttc gtgtggacct ggctcatcac cgcctacctg ctcatcgccg tgatgccggt
    10621 tctcgccggc tcggtgacga tgctcctcac cgaccgccac ttcggcacct cgttcttcaa
    10681 cgcggccggc ggcggcgatc ccacgatgtt catgcacatc ttctggttct tcgggcaccc
    10741 ggaggtgtac atcatgatcc tgccggcctt cggcgtgatc tcggcgatca tcccggcctt
    10801 cgcgcgcaag ccgctgttcg gctacgcctc gatggtctac gccacggcct cgatcgcgat
    10861 cctgtcgttc atcgtgtggg gccaccacat gttcacggtg ggcatgcccg cggcaggcaa
    10921 cctgttcttc atgtacgcga ccatgctcat cgcggtgccc acgggcgtga agatcttcaa
    10981 ctggatcgcg acgatgtggc gcggctcgat gaccttcgag acgccgatgc tcttctcgat
    11041 cggattcctg tgcctgttca cgatcggcgg cttctcgggc ctggtgctcg ccatcacgcc
    11101 cgtggacatc acgctgcaca acacctacta cgtggtggcg cacttccact acgtgctggt
    11161 ctccggagcg ctcttcgcga tcttcgccgg cgtgtacttc tggctgccca agtggaccgg
    11221 caacatgtac cacgagggcc tgggcaagct gcacttctgg ctgtcggtga tcggcttcaa
    11281 catcgccttc ttcccgcagc acttcctcgg gctcgcgggc atgccgcgcc gcatccccga
    11341 ctacgcgctg cagttcgccg agtggaacat gtggtcgtcc atcggcgcgt acatcttcgg
    11401 tttctcgcag ctgctcttcc tctacgtggt gatcgagtgc atccgatccg gccgcaagtc
    11461 ggagcagagg ccgtgggagg gcgcggactc gctcgagtgg acgcacctgc cgtgccccgc
    11521 gccgtaccac acgttcgaga cggccccggt cctcaagtga cgggaatcgt ccccatggcc
    11581 gaaggccgca agccgacacc cgagcagaag cggcgcgcgg tcgtgagcgc cgtcgtgctg
    11641 gccctgatgg tggcggccat ctacctcacc gtcgtgctca aggtcgcgct gtgacggacg
    11701 ccgtcctcgc cgcccgcaac cgccagatga tgggcaagct cctggtcatc gcggtggcca
    11761 tgctcgggtt cggcttcgcg atggtgccga tgtaccggca gatctgcgag tcgctcggca
    11821 tctcgcaggg ccgcgtcgtg ggcgcgacga acacgcaggt ggacgcgagc cgctcggtga
    11881 cggtggagct gctcgcctcg agcgcggggc tgccgtggcg cttcgagtcg ctcgagcgcg
    11941 aggtgaagct gcaccccggg cagctcgtca ccgtcaacta ccgggtggtg aacacgctcg
    12001 accggccggt gacggcgcac gcggtgatga acgccgcacc cgccgacgcc ggccagtaca
    12061 tcgagaagca ggcgtgtttc tgcttctcca cccagacgct cgccgccggg gaggagaggg
    12121 tgatgcccgt ggtgttcctg gtgagcccca gggccccgcg ggacatgacg acgatctcgc
    12181 tttcctacac cttcttcgag cagcccggga gcgcatcatg atggacgcct tcaaggcggt
    12241 cttctggagc ttcttcggca tccggaggaa caaggagtac gaggcagacg ccaagcgcct
    12301 cacgccgcag gcggtgatcg cggcgggcat cgtgacggcg ctggtggtcg tgctggcgat
    12361 cttcgggctc gtgaagctcg tcacgcgcta gccggcaaac ggattcgaac caggcaatac
    12421 aagggagtca caacgtgggc aacgcacaac ctggcggcta ctactacgca cctccttccc
    12481 attggccgat cgtggggtcc gcggcactgc tgctgatggc catcggcggc gtgctgctca
    12541 tgaacagcat cggcggcggc gggatcgtct tcggaatcgg cgcggcggtc ctcgtctaca
    12601 tgctcttcgg gtggttcggc acggtgatcc gcgaaagcga gggagggctg ttcaacaagc
    12661 aggtcgacat gtccttccgc tggagcatga gctggttcat cttctcgga
//
LOCUS       DASUEV010000035         6796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_348655, whole genome shotgun sequence.
ACCESSION   DASUEV010000035 DASUEV010000000
VERSION     DASUEV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348655"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /gene="tuf"
                     /locus_tag="VFV55_01085"
     CDS             <1..123
                     /gene="tuf"
                     /locus_tag="VFV55_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX4942912.1"
                     /translation="DNIKMVVTLIAPIAMEDGLRFAIREGGRTVGAGVVSKILE"
     gene            139..214
                     /locus_tag="VFV55_01090"
     tRNA            139..214
                     /locus_tag="VFV55_01090"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:172..174,aa:Trp,seq:cca)
     gene            231..578
                     /gene="secE"
                     /locus_tag="VFV55_01095"
     CDS             231..578
                     /gene="secE"
                     /locus_tag="VFV55_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX4942913.1"
                     /translation="MAEKIKIAAAALIAVAGLWGFYAISGMATIVRLGVLIGAFAVAA
                     GVMWFTQAGRDFVVFSRESKDEAKRVVWPTRKETLQTTGIVFAFVFIMAMMLWMIDAG
                     LLWITQRLLGQGG"
     gene            580..1113
                     /gene="nusG"
                     /locus_tag="VFV55_01100"
     CDS             580..1113
                     /gene="nusG"
                     /locus_tag="VFV55_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX4942914.1"
                     /translation="MGMRWYVVHAYSGQEKSIQAALIDRIKRSGMADLFGQVLVPTEE
                     VVEMKSGQKALSERRFFPGYILVQMEMTDDTWHLVKSTPKVTGFIGGTAMKPTPISDK
                     EVENILHQVQEGVEKPKPKVLFEVGESVRVTEGPFTDFNGNVEEVNYEKSKIRVSVLI
                     FGRATPVELDFGQVEKA"
     gene            1257..1688
                     /gene="rplK"
                     /locus_tag="VFV55_01105"
     CDS             1257..1688
                     /gene="rplK"
                     /locus_tag="VFV55_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX4942915.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKVEPGLMLPIVITAFADKSFTFIIKTPPAPVLIKRALKLEKGSSKPHAEKVGKLT
                     RAQAEEIARTKMPDLTAADLDAAVRTIAGSARSMGVEVEGV"
     gene            1691..2404
                     /gene="rplA"
                     /locus_tag="VFV55_01110"
     CDS             1691..2404
                     /gene="rplA"
                     /locus_tag="VFV55_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX4942916.1"
                     /translation="MANSKRVKTLTSLVDRTKAYALGDALDLAKKTATAKFDESIDVA
                     VNLGIDSKKSDQVVRGSVVLPRGTGKKVRVAVFAQGANAEAATAAGADVVGFDDLAER
                     IKGGFMEFDVVIATPDAMKVVGPLGQVLGPRGLMPNPKVGTVTPNVSEAVKNAKAGQV
                     QYRADKAGIVQCTIGRASFPLEALAQNFEALIGAINKSKPATTKGVYLKKVSVSSTMG
                     VGIRVDHSTLSGASAGAAQ"
     gene            2686..3216
                     /gene="rplJ"
                     /locus_tag="VFV55_01115"
     CDS             2686..3216
                     /gene="rplJ"
                     /locus_tag="VFV55_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX4942917.1"
                     /translation="MSLNLESKKEVVAEVSARLAKAQTIVLAEYRGLPVEKITQLRAR
                     ARASGVYLRVLKNTLARRAVQGTPFEKLADQMVGQLAYGISDDPVAAAKVLHAYAKEN
                     DKLVIKGGAMPNQMMSAKEVGNLAMLPGREELLAMLMGTMQAPATKFVQTLNEVPGRF
                     VRTLAALRDQKEKLAA"
     gene            3269..3643
                     /gene="rplL"
                     /locus_tag="VFV55_01120"
     CDS             3269..3643
                     /gene="rplL"
                     /locus_tag="VFV55_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX4942918.1"
                     /translation="MAIAKQEILDAIASMTVLELSQLIKEMEEKFGVSAAAAAVAVAA
                     PAAAAAAVEEKTEFDVILLAAGENKVNTIKAVREVTGLGLKEAKDLVDGAPKPVKEGV
                     NKADADALKKKLEEAGAKVEVK"
     gene            3902..>6796
                     /gene="rpoB"
                     /locus_tag="VFV55_01125"
     CDS             3902..>6796
                     /gene="rpoB"
                     /locus_tag="VFV55_01125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX4942919.1"
                     /translation="MTYTFTEKKRIRKSFAKRAAVLDVPFLLATQLDSYADFLQAEVQ
                     PGQRRDAGLQSAFTSVFPISSHSGNARLEFVSYALGEPPFDVVECQQRGLTFASPLRA
                     KVRLTIMDREASKPTVKEVKEQEVYMGEIPLMTRNGSFVVNGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDFLYFRVDRRRKMPVTILLKALG
                     YSLERILAEFFVFDTFHFGKKGIEFEVVPERMKGEIARFDIVTKTGKAIVQKDKRITV
                     KHIREMEAAGMKKLVVEPDFLLGRTLAKNLVNKETGEIVANANEEITETLLKKITDAG
                     VEEVRTLYTNDLDQGPYISQTLRIDESVDQLNAQVAIYRMMRPGEPPTEDAVKTLFNG
                     LFFSEDRYDLSDVGRMKFNRRVGRDELTGPMTLSTDDIVAVIRILVELRNGRGEIDDI
                     DHLGNRRVRSVGELAENQFRSGLVRVERAVRERLSQAESENLMPHDLINAKPVSAAIK
                     EFFGSSQLSQFMDQTNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSLALYARTNEYGFIETPYRRVKDSKVTKDIEFLSAIEEGKYVIAQA
                     NAELDKGGHFVDELVSCRHRNEFALSGPDKIEYIDVAPSQIVSVAASLIPFLEHDDAN
                     RALMGSNMQRQAVPCLRAEKPFVGTGIERTVAIDSGTAVQARRGGRIDYVDAGRIVVR
                     VNDNETSAGKAGVDIYNLTKYTRSNQNTNINQRPLVKVGNLIAAGDVIADGASTDMGE
                     LALGQNLLVAFMPWNGYNFEDSILISERVVAEDRYTSIHIEELVVVARDTKLGPEEIT
                     RDISNLSERMLARLDESGIVYIGAEVEAGDVLVGKVTPKGETQLTPEEKLLRAIFGEK
                     ASDVKDTGLRVPSGISGCVIDVQVFTREGIQRDKRAQQIIDDQLKDYRKDL"
BASE COUNT         1315 a         2227 c         2250 g         1004 t
ORIGIN      
        1 gacaacatca agatggtggt gacgctgatc gcgccgatcg cgatggagga cggcctgcgc
       61 ttcgccatcc gcgagggcgg ccgcaccgtc ggtgccggcg tcgtttcgaa gatcctggag
      121 taaacgagtt gcaaggttag gccagtagct caattggcag agcggcggtc tccaaaaccg
      181 caggttgggg gttcgagacc ctcctggcct gccacccggt ggcagaggac atggcagaaa
      241 agataaagat agcggcagcg gcgttgattg cagtggcggg cctgtggggc ttctacgcga
      301 tttccggaat ggctaccatc gtgcgcctcg gcgtgctgat cggcgcgttc gcggtcgccg
      361 cgggcgtgat gtggttcacg caggcgggcc gcgacttcgt cgtcttctcc cgggagtcca
      421 aggatgaagc gaagcgcgtc gtctggccca cgcgcaagga gacgctgcag accacgggga
      481 tcgtcttcgc gttcgtgttc atcatggcca tgatgctgtg gatgatcgat gcggggctgc
      541 tctggatcac gcagcgcctg ctcgggcagg gtggctgaca tgggcatgcg ctggtacgtg
      601 gtccacgcct actccgggca ggagaagagc atccaggcgg cgctgatcga ccggatcaag
      661 cgctcgggca tggccgacct gttcggccag gtgctcgtcc ccacggagga agtggtcgag
      721 atgaagagcg ggcagaaggc gctgagcgag cgcaggttct ttcccggcta catcctggtg
      781 cagatggaga tgacggacga cacctggcac ctggtgaaga gcacgcccaa ggtgaccggc
      841 ttcatcggcg gcacggcgat gaagcccacg cccatctccg acaaggaggt cgagaacatc
      901 ctgcaccagg tgcaggaagg cgtcgagaag cccaagccga aggtgctctt cgaggtgggc
      961 gagtcggtgc gcgtgaccga ggggccgttc acggacttca acggcaacgt cgaggaagtg
     1021 aactacgaga agagcaagat ccgggtttcg gtgctgatct tcgggcgcgc gaccccggtg
     1081 gagctggatt tcgggcaggt cgagaaggcc tgaccgtggt cgaggaacga gctgtccctt
     1141 gaacaacttt tcgccgcgtc gatggacgcg gcgcgcggag gccgggcgcg ttccccggtc
     1201 gaactggacg gggagggcgc gctgcaggcg cccgctggaa cccggggagt gccatcgtgg
     1261 caaagaagat cgtcggcttc atcaagctgc aagtgcctgc gggcaaggcg aatccctcgc
     1321 cgcccatcgg gccagcgctc ggccagcgcg gcctcaacat catggaattc tgcaaggcct
     1381 tcaacgcgca gacgcagaag gtcgagccgg gcctcatgct cccgatcgtc atcacggcgt
     1441 tcgcggacaa gagcttcacc ttcatcatca agacgccccc ggcgccggtg ctgatcaaga
     1501 gggcgctcaa gctcgagaag ggcagcagca agccgcacgc cgagaaggtc ggcaagctca
     1561 cgcgcgccca ggccgaggaa atcgccagga ccaagatgcc cgatctcacg gcggccgatc
     1621 tcgatgccgc cgtgcgcacg atcgccggca gcgcccgcag catgggcgtc gaagtggaag
     1681 gagtctgacc atggccaact ccaagcgcgt gaagaccctc acgtccctcg tggaccgcac
     1741 gaaggcctac gccctcggcg atgcgctcga tctcgccaag aagaccgcca cggcgaagtt
     1801 cgacgagtcg atagatgtcg cggtgaacct cggcatcgat tccaagaagt ccgaccaggt
     1861 cgtgcgcggc agcgtcgtgc tgccccgcgg aacgggaaag aaggtccggg tggcggtgtt
     1921 cgcgcagggc gcgaacgccg aggccgccac ggccgccggc gccgacgtgg tcggtttcga
     1981 cgacctcgcc gagcgcatca agggcgggtt catggaattc gacgtggtca tcgccacgcc
     2041 ggacgcgatg aaagtcgtcg gccccctcgg ccaggtgctc ggcccgcgcg gcctgatgcc
     2101 caacccgaag gtcggcacgg tgaccccgaa cgtgagcgag gcggtgaaga acgccaaggc
     2161 gggccaggtg cagtaccgcg ccgacaaggc gggaatcgtg caatgcacga tcggccgcgc
     2221 gtcgttcccg ctcgaggcgc tcgcgcagaa cttcgaggcg ctcatcgggg cgatcaacaa
     2281 gtcgaagccc gccaccacca agggcgtgta tctcaagaag gtgtccgtct ccagcacgat
     2341 gggggtgggc atccgcgtgg atcattcaac gctgtccggc gcttccgccg gcgcggcgca
     2401 gtagctttgg gccgccgccg gcccgcgagg gccggcggcg ggttatccaa gaccgtaggc
     2461 gccttccggg tagccccagt ccgacggggc gagccaaagg ggctcaatca aggggaaccg
     2521 accccgaagc ctacgcagat agcgaaccca agacaggatt ctcgcgcccg attcccgggc
     2581 gcaaccggtc tcctgacgaa aggtcgctat ggcaggtgcg gcgcattgcg ggcattccca
     2641 agggcccgtg aaagcggcgc atcgattagc tgagggagaa agaccttgag tctgaatcta
     2701 gaaagtaaga aggaagtcgt ggcggaagtc tccgcccggt tggccaaggc gcagaccatc
     2761 gtgctcgccg agtatcgcgg ccttccggtc gagaagatca cgcagctgcg cgccagggcg
     2821 cgcgcgtccg gcgtctacct tcgcgtgctg aagaacaccc tcgcgcgccg cgcggtccag
     2881 ggaacgccgt tcgagaagct cgccgaccag atggtcggcc agctcgccta cggcatttcg
     2941 gacgatccgg tggcggcggc gaaagtgctc cacgcctatg cgaaggaaaa cgacaagctc
     3001 gtcatcaagg gcggagcgat gcccaaccag atgatgagcg cgaaggaggt cggcaacctc
     3061 gccatgctcc cgggccgcga ggaactcctc gccatgctca tgggcacgat gcaggcgccg
     3121 gccaccaagt tcgtccagac gctgaacgag gttccgggca ggttcgtccg gacgctcgcc
     3181 gcccttcgcg accagaaaga gaagctggcg gcctgaccgc cgcgacccca aaccaacgac
     3241 cactgaatca agtacaggag aacgaaacat ggctatcgcc aagcaggaaa tcctcgacgc
     3301 aatcgcaagc atgaccgtcc tcgagctctc gcagctcatc aaggaaatgg aagagaagtt
     3361 cggcgtgtcc gccgccgcgg ccgccgtcgc cgttgccgcg cccgcagcgg cagccgccgc
     3421 cgtcgaggag aagacggagt tcgacgtgat cctcctcgcc gccggcgaga acaaggtgaa
     3481 caccatcaag gcggtgcgcg aagtgacggg cctggggctg aaggaagcca aggacctcgt
     3541 cgacggcgcg cccaagcccg tgaaggaagg cgtgaacaag gccgacgccg acgcgctcaa
     3601 gaagaagctg gaagaagccg gcgccaaggt cgaggtgaag taactgttgt cggttcctga
     3661 tgtccaacgg cgttgagcgg caccgccccg gcggtgtccg ctcgagcgcc gttccagatg
     3721 tcaaatcgaa gcccttccgg gtttcgattt gccttctgaa gcgactgcag aaggtgaggt
     3781 ttggtcgggc cgcatccctt gcggctgtcc gccagcggct ggtagaggcc agccaccagg
     3841 ccatgagcac cagtcgggtg cagcgggcgc gcacgcgccc gcgggtattg acggagccat
     3901 catgacctac acgttcaccg agaagaagcg cattcgcaag agtttcgcca agcgggcggc
     3961 cgtgctcgac gtgcccttcc tcctggccac gcagctcgat tcgtacgccg atttcctgca
     4021 ggccgaggtc cagcccggcc agcgccgcga cgccggcctg cagtcggcct tcacctccgt
     4081 gttcccgatc tccagccact cgggcaatgc gcgcctggag ttcgtgagct acgccctggg
     4141 cgagcccccg ttcgacgtgg tcgaatgcca gcagcgcggc ctgaccttcg cgagcccgct
     4201 gcgcgccaag gtgcgcctca cgatcatgga ccgcgaggcg agcaagccca cggtcaagga
     4261 ggtgaaggag caggaggtct acatgggcga gatcccgctc atgacgcgaa acggttcctt
     4321 cgtcgtgaac ggcaccgagc gcgtgatcgt gagccagctg caccgctcgc ccggcgtgtt
     4381 cttcgagcac gaccgcggca agacccactc ctcgggcaag ctcctcttct cggcccgcgt
     4441 gattccctat cgcggctcgt ggctcgactt cgagttcgat cccaaggact tcctgtactt
     4501 ccgcgtcgac cgccggcgca agatgccggt cacgatcctg ctgaaggcgc tggggtactc
     4561 cctcgagcgc atcctcgccg agttcttcgt gttcgacacg ttccacttcg gcaagaaggg
     4621 catcgagttc gaggtcgtgc cggagcgcat gaagggcgag atcgcccggt tcgacatcgt
     4681 caccaagacc ggcaaggcga tcgtccagaa ggacaagcgc atcacggtga agcacatccg
     4741 cgagatggaa gccgcgggga tgaagaagct cgtcgtcgag cccgacttcc tgctcggccg
     4801 cacgctggcg aagaacctgg tcaacaagga aacgggcgag atcgtcgcca acgccaacga
     4861 ggaaatcacc gagacgctcc tcaagaagat caccgacgcc ggcgtggagg aagtgcgcac
     4921 gctctacacc aacgacctcg accagggccc gtacatctcg cagacgctgc gcatcgacga
     4981 atccgtcgac cagctcaacg cccaggtcgc gatctaccgc atgatgcgcc ccggcgagcc
     5041 gccgaccgag gacgcggtga agacgctctt caacgggctc ttcttcagcg aggaccgcta
     5101 cgacctgtcc gacgtcggcc gcatgaagtt caaccgccgc gtgggcaggg acgagctcac
     5161 cggcccgatg acgctctcca ccgacgacat cgtcgccgtc atccgcatcc tcgtggagct
     5221 gcgcaacggc cgcggcgaga tcgacgacat cgaccacctc ggcaaccgcc gcgtgcgctc
     5281 ggtgggcgag ctcgccgaga accagttccg ctccggcctg gtgcgcgtcg agcgcgcggt
     5341 gcgcgagcgc ctttcgcagg ccgagagcga gaacctgatg ccgcacgacc tgatcaacgc
     5401 gaagcccgtt tccgccgcga tcaaggagtt cttcggctcc agccagctct cgcagttcat
     5461 ggaccagacg aacccgctct cggagatcac gcacaagcgc cgcatctcgg cgctcggccc
     5521 cgggggcctc acgcgggagc gcgccggctt cgaggtgcgc gacgtgcacc cgacccacta
     5581 cggccgcgtg tgcccgatcg agacgcccga aggcccgaac atcggcctca tcaactcgct
     5641 ggccctctac gcccgcacga acgagtacgg cttcatcgag acgccctatc gccgcgtgaa
     5701 ggactccaag gtcacgaagg acatcgagtt cctgtccgcg atcgaggaag gcaagtacgt
     5761 gatcgcgcag gcgaacgccg agctcgacaa gggcggccat ttcgtcgacg agctggtctc
     5821 ctgccggcac cggaacgagt tcgcgctctc cggcccggac aagatcgagt acatcgacgt
     5881 ggccccgtcg cagatcgtgt cggtggccgc ctccctcatc ccgttcctcg agcacgacga
     5941 cgcgaaccgc gccctgatgg gctccaacat gcagcgccag gcggtcccgt gccttcgcgc
     6001 ggagaagccg ttcgtgggca ccggcatcga gcgcaccgtc gcgatcgact cgggcaccgc
     6061 ggtgcaggca cgccgcggcg ggcgcatcga ctacgtcgac gcgggccgca tcgtggtccg
     6121 ggtgaacgac aacgagacga gcgccggcaa ggcgggcgtg gacatctaca acctcaccaa
     6181 gtacacgcgc tccaaccaga acacgaacat caaccagcgc ccgctggtga aggtcggcaa
     6241 cctgatcgcc gccggcgacg tgatcgccga cggcgcctcg accgacatgg gcgagcttgc
     6301 cctcggccag aacctcctgg tcgcgttcat gccgtggaac ggctacaact tcgaggactc
     6361 catcctcatc tccgagcgcg tggtcgccga ggaccgctac acctcgatcc acatcgagga
     6421 actggtggtc gttgcccgcg acacgaagct cgggccggag gaaatcacgc gcgacatctc
     6481 caacctgtcc gagcgcatgc tcgcgcggct ggacgagtcg ggcatcgtgt acatcggcgc
     6541 cgaggtggag gcgggcgacg tgctcgtcgg caaggtcacg cccaagggcg agacgcagct
     6601 cacgccggag gaaaagctcc tgcgcgcgat cttcggcgag aaggcctccg acgtgaagga
     6661 caccggcctc agggtcccgt ccggcatttc cggctgcgtg atcgacgtgc aggtattcac
     6721 gcgcgagggc atccagcgcg acaagcgcgc ccagcagatc atcgacgacc agctcaagga
     6781 ctaccgcaag gacctg
//
LOCUS       DASUEV010000036         9400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_697785, whole genome shotgun sequence.
ACCESSION   DASUEV010000036 DASUEV010000000
VERSION     DASUEV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697785"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VFV55_01130"
     CDS             <1..959
                     /locus_tag="VFV55_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX4942920.1"
                     /translation="PFSAMDALSEVLRLASLTGHVLADATAGGAWCVAVPAASSRAFA
                     HVVLEGECTLQAGDSGPIALRAGDVAFAARGEGHLLGSDLSIEPTPFAALLRPPVAGE
                     LAPVTLGGTGRRTRWVTLAISADRHLAEPLFASLPPVLKASLRGTAPLGWLSDSLGLC
                     LSGSDAPRPGGAAALSRVAELVLIEALRRHVEELPPGGSGWLAALNDRYVGAALALVH
                     GRPGEHWTVERLARQVGLSRSALAERFSLVVGEPIFGFLTRVRLQLAAHKLATTTHSI
                     EAIAEGAGYEAVSSFSRAFKRTFGKPPTVWRRKSRRRRPALA"
     gene            complement(951..2057)
                     /locus_tag="VFV55_01135"
     CDS             complement(951..2057)
                     /locus_tag="VFV55_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX4942921.1"
                     /translation="MALKRPKWSPGTVVMLEHVSKVLKGNALGDPHVRTVAAWLPPQY
                     DEGATRGRGRRFPVLVDMVGFTGSGLSHVAWKSFSENVPERAARLVHEGKMGPAIIVF
                     PDCFTALGGNQYVNSSAIGNYADYLTKEIVPFVDREFRTRASREHRGCFGKSSGGYGA
                     IIHGMKYASTWGAIADHSGDAYFDFVYHHDWPNTLNELAKYRPGKFVPGAYDARKMEK
                     RCDRGLDDGRVKAFLKAVWGKAKLSMAEGHAIMNVCMAASYDPDPKAPNGFRLPFNLE
                     TGELLKARWKHWQANDPVNLVRKYRKNLASLRGIYIDCGSRDQYHIHYGSRILSKRLQ
                     QAGIRHTYEEFEDNHSDIDYRMDASLPFLARALR"
     gene            complement(2211..2771)
                     /gene="efp"
                     /locus_tag="VFV55_01140"
     CDS             complement(2211..2771)
                     /gene="efp"
                     /locus_tag="VFV55_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX4942922.1"
                     /translation="MKTAQELRSGNVFMVGKDPMVVLRAEYNKGGRNAATMKMKMKNL
                     LTGSNTETVYKADEKFELLMLERKEVTYSYFADPMYVFMDGDFNQYEIEKESLGDALN
                     YLEEGMPCEATFYEGRAISLDIPTTVVREIIYTEPAVRGDTSGKVLKPAKLANGHEVS
                     VPAFCATGDKIEIDTRTNEYKRRVNA"
     gene            complement(2827..3984)
                     /gene="earP"
                     /locus_tag="VFV55_01145"
     CDS             complement(2827..3984)
                     /gene="earP"
                     /locus_tag="VFV55_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEX4942923.1"
                     /translation="MATPPERWDIFCRVVDNYGDVGVAWRLARCLAREHGKRVRLFLD
                     DLTVLARLRPDADPARAEQRIDSVDIWCWDERFGGREPVPSDAVADVVVETFGCDTPA
                     PYVLAMAARRPRPRWINLEYLSAEEWVEGSHALPSPHPRLAPLTRHYFFPGFTARTGG
                     LLREGDLLERRDAFQADRAAQAQFWRALTGADPQHGALKVSLFGYAGAPYVALLDAMA
                     HHAGPAWVAAPEGVASAALTAWLQGKPHGERGAVTAFSIPFLDQDRYDELLWACDLNF
                     VRGEDSFVRAQWAARPFVWHIYPTDDDAHWVKLAAFLARYTAGMDRAHAARVTAFWEA
                     WNRGEDMARTWPVFDAALAAVSPHAEDWATRLAGQRDLASQLVDFADNVLK"
     gene            4051..4413
                     /locus_tag="VFV55_01150"
     CDS             4051..4413
                     /locus_tag="VFV55_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4942924.1"
                     /translation="MKPDEMDAVFSALASEPRRRILDILKKEPGCNVSRVCEYFDIGR
                     VAVMKHLAALGRANLVISAKAGRERQLWFNPVPIHMIYDRWTTEFSDYWAGRLARAKY
                     RLENAPVPLARAKGGARG"
     gene            4406..4927
                     /locus_tag="VFV55_01155"
     CDS             4406..4927
                     /locus_tag="VFV55_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX4942925.1"
                     /translation="MAEPNKAVFRIVIAGTMEAVFRELTKTGEPQGAVFNSMLTLDGP
                     GLVRGRKMQMRTVSGRHAMVVGEVVEHQPPARFAHTHRFTQYDDPSCTVVYDLKKVDG
                     GVEVTLTVENIPAGTGTEKEMMKGGDFILRNLKSIVETGRPPFGTRLMYAMFGALEFV
                     LPGKTKSEHWPLQ"
     gene            4999..5583
                     /locus_tag="VFV55_01160"
     CDS             4999..5583
                     /locus_tag="VFV55_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX4942926.1"
                     /translation="MRRLIGALIIALYPIAAVAQDRTIVKVASVKAPVGEVWKAWTTR
                     EGIRSFFAPDARIEAHPGGPFEVHFNPYAKPGLKGADDMVFLALQENRMLSFTWNAPP
                     HLPDVRGQRTSVTVRLKSAGEGLTEVRLTHAGWGDGGQWDQSFDYFDKAWGRVLANLE
                     KRFVDGPVDWAPWLKQMRDHQDAEDAKAAGAKKP"
     gene            5759..5962
                     /locus_tag="VFV55_01165"
     CDS             5759..5962
                     /locus_tag="VFV55_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX4942927.1"
                     /translation="MATGTVKWFNDSKGFGFITPEGGGEDLFAHFSEIQAAGFKTLKE
                     NQRVEFEVKMGPKGKQATAIKPL"
     gene            complement(6013..6852)
                     /locus_tag="VFV55_01170"
     CDS             complement(6013..6852)
                     /locus_tag="VFV55_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX4942928.1"
                     /translation="MPRLLFDKPGGKIRLDDIPADPPKGMTREKAEKRFDSLGRELFS
                     LQDAMYGAKVNSVMVVLQGRDGAGKDGAIKHVVGCLNPRGVIVTSFGVPTPEERQHDF
                     LWRVHRHAPRLGEFSIFNRSHYEDVLVVRVHDLAPKAVWKERFGHIADFEELLAEHGT
                     IVLKYFLHITKEEQEGRLLEREVSPDTAWKLNPNDWKERDYWDEYTRAYEDAISRTSA
                     GHAPWTIVPANAKWYRNLVIAESIVEALRARRKDWEKTLAAMGKAGRAGLEAYRAGLP
                     AAK"
     gene            7317..9209
                     /gene="glgB"
                     /locus_tag="VFV55_01175"
     CDS             7317..9209
                     /gene="glgB"
                     /locus_tag="VFV55_01175"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX4942929.1"
                     /translation="MQTSKITDLDIHLFREGTHARLYRCLGCQLDPGGQGGRFAVWAP
                     NARAVSVIGDWNGWNAGADRLSPRADGSGIWEGEVAGVRSGQAYKFRIESHALGHVAE
                     KADPFAFLAECPPATASRAWSLDYEWGDDGWMASRARRNALDAPMSIYEVHAGSWRRK
                     DGAFLGYRELAHQLAQYVRDAGFTHVELMPVTEHPFYGSWGYQTTGYFAPTARYGTPQ
                     DFMYFVDHLHRNDIGVILDWVPSHFPADAHGLGFFDGTYLYEHADPKQGFHPEWNSSI
                     FNYGRNEVRAFLLSSALFWLDRYHLDGLRVDAVASMLYLDYARQEGEWIPNVHGGKEN
                     LAAIDFLRTLNTAVYRDYPDTMSIAEESTAWPMVSRPAWLGGLGFGLKWNMGWMHDTL
                     AYLSEDPINRKYHHGKLTFSLIYAFHENFVLPLSHDEVVHGKGSLVNKMPGDTWRQFA
                     NLRALYGYMWAHPGKKLLFMGCEFGQRREWMHDGELEWWVLSLAEHEGVRRWVADLNR
                     FYREEPALWQVDFDPHGFQWIEANDAENSVISFLRRPRGDGPVVVVACNLTPVPRENY
                     SLGVPMPGRWAEALNSDATLYGGAGWGNLGGLDAAATGAHGQPFSLTVTLPPLSTVIF
                     RSGDRG"
     gene            9202..>9400
                     /locus_tag="VFV55_01180"
     CDS             9202..>9400
                     /locus_tag="VFV55_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942930.1"
                     /translation="MVERASAAETQPAGRDEAASAAADALWYKDAIIYQLHIKAFFDS
                     NDDGVGDFPGLASRLDYVQSLG"
BASE COUNT         1522 a         3177 c         3137 g         1564 t
ORIGIN      
        1 accccttttc agctatggac gccctctccg aagtcctgcg cctcgcaagc ctcaccggcc
       61 acgtgctcgc cgatgccacc gcgggcggag cgtggtgcgt ggcggtgccg gcagccagtt
      121 cgcgcgcctt cgcgcacgtc gtgctcgaag gcgagtgcac gctccaggct ggggattccg
      181 ggccgatcgc actgcgcgca ggcgacgtcg cgttcgccgc acgaggggaa ggccacctcc
      241 tgggctcgga cctgtccatc gagccgacgc cgttcgccgc gctcctgcgt ccgcccgtcg
      301 cgggggaact cgcgcccgtc acgctgggcg gaacgggccg gcgaacgcgc tgggtgacgc
      361 tcgcgatctc ggcggacagg cacctggccg aaccgctttt cgcttccctg ccgcccgtgc
      421 tcaaggcgag cctgcggggc acggcgccgc tcgggtggct ttccgattcg ctcgggctgt
      481 gcctctccgg cagcgacgcg ccgcgtcccg ggggcgcggc tgcgctctcg cgcgtggcgg
      541 aactggtgct catcgaggcc ctgcgccgcc acgtcgagga actgcctccc ggcggatcgg
      601 gttggctcgc ggcgctgaac gaccgctacg tgggcgcggc actcgcgctg gtgcatggcc
      661 ggcccgggga gcactggacc gtcgagcgcc tggccaggca ggtggggctc tcgcgctcgg
      721 cgctcgcgga gcgcttctcc ctcgtcgtgg gcgagcccat cttcggattc ctcacgcgcg
      781 tgcggctgca gctcgcggcg cacaagctcg ccaccaccac gcattcgatc gaggcgatcg
      841 cggagggcgc gggctacgag gccgtgtcct ccttctcacg cgcgttcaag cgcacgttcg
      901 gcaagcctcc gaccgtatgg cggcgcaagt cgagacgcag gcgcccggcg ctagcgtagc
      961 gcccgcgcga ggaacggcag ggaggcatcc atccggtaat cgatgtcgga gtggttgtcc
     1021 tcgaattcct catacgtatg gcggatcccc gcctgctgca ggcgcttcga gaggatcctt
     1081 gagccgtagt ggatgtggta ctggtcgcgc gagccgcagt cgatgtagat cccgcgcagc
     1141 gacgcgaggt tcttccggta ctttcgcacc aggtttaccg ggtcgttggc ctgccagtgc
     1201 ttccaccgcg ccttcagcaa ctccccggtc tcgaggttga atggcagccg gaagccgttg
     1261 ggcgctttcg ggtccgggtc gtagctcgcc gccatgcata cgttcatgat cgcgtggccc
     1321 tcggccatcg agagcttggc cttgccccac accgccttca ggaacgcttt cacgcggccg
     1381 tcgtcgagtc cgcgatcgca gcgcttttcc atctttctcg cgtcataggc gccgggcacg
     1441 aacttccccg gccggtattt cgccagttcg ttgagcgtgt tgggccagtc gtggtggtag
     1501 acgaaatcga agtaggcgtc gcccgagtgg tccgcgatcg cgccccaggt ggaggcgtac
     1561 ttcatgccat ggatgatggc gccgtagccg ccggaggact tgccgaagca gccgcgatgc
     1621 tcgcgcgatg cgcgcgtgcg gaattcgcgg tccacgaagg gaacgatctc cttcgtgagg
     1681 taatcggcgt aattgccgat cgcggaggag ttcacgtact ggttgccgcc cagcgccgtg
     1741 aagcagtccg ggaagacgat gatcgccggg cccatcttcc cctcgtgcac gaggcgggcc
     1801 gcgcgctcgg gcacgttctc gctgaagctc ttccatgcga catgcgagag gcccgagccg
     1861 gtgaacccca ccatgtccac gagcacggga aagcgccggc cgcggccgcg cgtggcgccc
     1921 tcgtcgtact ggggcggcag ccacgccgcg acggtgcgca cgtgcggatc gccaagggcg
     1981 ttgcccttga gtaccttgga aacgtgctcc agcatcacta ccgtgccggg actccacttc
     2041 gggcgcttca gggccatggg atcgctccat cacttgcgag aacgggagct tagcgggaaa
     2101 gcgaatggcg agggaaccgc agatgaacga acccgaaaag cgggggaacc acagaaaaca
     2161 caaggggcgc cgaagcgccc cttgtgtaat gccggtggtg gcggcgggcg tcaggcgttg
     2221 acgcgccgct tgtactcgtt ggtgcgggtg tcgatctcga tcttgtcccc ggtcgcgcag
     2281 aacgccggca cggacacttc gtggccgttg gcgagctttg ccggcttgag caccttgccc
     2341 gaggtgtcgc cgcgcacggc gggctcggtg tagatgatct cgcgcacgac cgtggtcggg
     2401 atatcgaggg agatcgcgcg cccctcgtag aaggtggcct cgcagggcat gccttcctcg
     2461 agatagttga gcgcgtcgcc caggctttcc ttctcgatct cgtactggtt gaagtcgccg
     2521 tccatgaaca cgtacatggg atcggcgaag taggagtaag tcacttcctt gcgttcgagc
     2581 atgaggagct cgaacttctc gtcggccttg tacaccgtct cggtgttgga accggtgagg
     2641 agattcttca ttttcatctt catggtggcg gcattgcgac cgcccttgtt gtactccgcg
     2701 cggagtacga ccatcgggtc cttgcccacc atgaacacat ttccgctgcg cagttcctga
     2761 gccgttttca taggtccgtc cgtgaattca tccgcccacc gtcgggtggg atatcgaaaa
     2821 agccttttat tttagcacgt tatccgcgaa atccaccagc tgcgatgcaa gatcgcgctg
     2881 gccggccagg cgggttgccc agtcctcggc gtgaggagac accgccgcca gggcggcgtc
     2941 gaacaccggc caggtacggg ccatgtcctc gccccggttc cacgcctccc agaatgcggt
     3001 tacccgggct gcatgggccc ggtccatgcc cgccgtgtag cgcgcgagga acgcggcgag
     3061 cttcacccag tgcgcgtcgt cgtcggtcgg gtagatgtgc cagacgaagg gacgcgccgc
     3121 ccactgcgcg cgcacgaagg aatcctcgcc gcgcacgaaa ttgaggtcgc aggcccagag
     3181 cagttcgtcg taccggtcct ggtcgaggaa ggggatgctg aaggcggtca cggcgccacg
     3241 ctccccgtgc ggcttgccct gcagccaggc ggtcagcgcc gcggatgcga ctccttcggg
     3301 cgcggccacc catgccgggc cggcatggtg cgccatcgcg tcgagcaggg ccacgtaggg
     3361 tgccccggca taaccgaaaa ggctcacctt gagcgccccg tgctgcgggt cggcaccggt
     3421 cagcgcgcgc cagaactgcg cctgcgccgc cctgtcggcc tggaaggcgt cgcgccgctc
     3481 gaggagatcg ccctcgcgaa gcaagccgcc cgtgcgagcg gtgaagccgg ggaagaagta
     3541 gtgccgcgtg aggggcgcca gccgcgggtg cggcgaaggc agcgcgtggc tgccctcgac
     3601 ccattcctcc gcgctcaggt attcgagatt gatccagcgc ggccgcggcc tcctcgcggc
     3661 catggcgagc acgtaaggcg cgggcgtgtc gcagccgaag gtctccacca ccacgtcggc
     3721 aaccgcgtcc gacgggaccg gctcgcgccc gccgaagcgc tcgtcccagc accaaatgtc
     3781 gacggaatcg atgcgctgtt cggcacgcgc ggggtccgcg tccggccgca ggcgcgcgag
     3841 gacggtcagg tcatcgagga agagtcgcac cctcttgcca tgttcgcgcg cgaggcatcg
     3901 cgccagccgc cacgccacgc ccacgtcgcc gtagttgtcg accacgcggc agaagatgtc
     3961 ccagcgctcg gggggagtgg ccatggagaa attctatctc ccggctccgc ttgacaggcc
     4021 cttggctgat atgtcacctt tcggtgacct atgaaaccgg acgagatgga tgccgtattc
     4081 tccgcgctcg ccagcgagcc gcggcggcgc atcctggaca tcctcaagaa ggagccgggg
     4141 tgcaacgtga gccgcgtgtg cgagtacttc gacatcgggc gcgtggcagt gatgaagcac
     4201 ctggcggcgc tcggtcgcgc caacctcgtc atctccgcga aggccgggcg cgagcggcag
     4261 ctgtggttca acccggtgcc gatccacatg atctacgacc gctggacgac cgagttcagc
     4321 gactactggg cggggcggct cgcgcgcgcg aagtaccggc tcgagaacgc cccggttccc
     4381 ctggccagag cgaagggagg cgcacgtggc tgaaccgaac aaggcggtat tccggatcgt
     4441 gatcgcggga acgatggaag cggtgttccg cgaactcacc aagacgggcg agccgcaggg
     4501 cgccgtgttc aactcgatgc tgacgctcga tgggccgggc ctggttcgcg gccggaagat
     4561 gcagatgcgc acggtgagcg gccggcacgc catggtggtc ggcgaggtcg tcgagcacca
     4621 gcctcccgcg cgcttcgcgc atacccaccg tttcacccag tacgatgacc cctcctgcac
     4681 cgtggtctac gacctgaaga aggtcgacgg cggcgtggag gtcaccctca ccgtcgagaa
     4741 cattcccgcg ggcaccggca ccgagaagga aatgatgaag ggcggcgact tcatcctcag
     4801 gaacctcaag tcgatcgtcg agaccggccg cccgcccttc ggcacgcggc tcatgtacgc
     4861 gatgttcggc gcgctcgagt tcgtgctccc cggcaagacg aaatcggagc actggccgct
     4921 ccaatgaacc cggttactgt acccggtaca tgtacccggt acgtgtaccg ggtacaacga
     4981 atgaggagga tatcgtccat gagaaggttg atcggcgcgc tgatcatcgc cttgtacccc
     5041 atcgcggctg tcgcgcagga tcgcaccatc gtgaaggtcg ccagcgtgaa ggcgccggtg
     5101 ggcgaggtgt ggaaggcgtg gaccacgcgc gagggcatca ggtccttttt cgcccccgac
     5161 gcccgcatcg aggcccatcc gggagggcct ttcgaggtcc acttcaatcc ctacgcgaaa
     5221 cccggcctga agggcgcgga cgacatggtc ttcctcgccc tgcaggaaaa caggatgctc
     5281 tccttcacct ggaacgcccc gccgcacttg ccggacgtgc gcggccagcg aaccagcgtc
     5341 accgtgcggc tgaagtccgc cggggagggc ctgaccgagg tgcgcctgac ccacgccggc
     5401 tggggcgacg gcggccagtg ggaccagtcc ttcgactact tcgacaaggc gtggggccgc
     5461 gtgctcgcca atctcgagaa gcgattcgtc gacggcccgg tcgactgggc gccctggctc
     5521 aagcagatga gggaccacca ggacgccgag gatgccaagg cggcgggcgc caagaaaccc
     5581 tgacgcgacg gtccgttttc gcgtatcatc gcgagcagca gtgattcagc ctgttcagca
     5641 gtaactcaag taccgccagc atcacggcgc ctctactcag ttcccgcctg gccaatctgt
     5701 gtcgcttgcg gtacgcgggt ccgcccgcat tttcaaattt gttcaaagga cacattacat
     5761 ggcaaccgga acagtcaagt ggttcaacga ttccaagggc ttcggcttca ttaccccgga
     5821 aggcggcggc gaggatctct tcgcgcactt ctcggaaatc caggccgcgg gcttcaagac
     5881 gctgaaggaa aaccagcgcg tcgaattcga agtcaagatg ggccccaagg gcaagcaggc
     5941 cacggcgatc aagccgctgt aagccgcgcc ccacgcagca attgagaacg gcagccttcg
     6001 ggctgccgtt ttttatttcg cggcgggcaa ccccgcgcga taggcctcga gcccggcacg
     6061 acccgccttg cccatcgccg cgagcgtctt ctcccagtcc ttgcggcggg cccgcagcgc
     6121 ctcgacgatc gactcggcga tgaccaggtt gcggtaccac ttcgcgttcg ccggcacgat
     6181 cgtccacggc gcgtgcccgg ccgacgtccg ggagatggcg tcttcgtagg cgcgcgtgta
     6241 ctcgtcccag tagtcgcgtt ccttccagtc gttgggattg agcttccacg cggtgtcggg
     6301 cgacacctcg cgctcgagca ggcggccctc ctgctcttcc ttcgtgatgt gcaggaagta
     6361 cttgaggacg atcgtgccgt gctcggccag cagttcctcg aagtcggcga tgtggccgaa
     6421 gcgctccttc cagaccgcct tgggcgcgag gtcgtggacg cggacgacca gcacgtcctc
     6481 gtagtgcgag cggttgaaga tggagaattc gcccaggcgc ggcgcgtggc ggtggacgcg
     6541 ccacaggaag tcgtgctggc gctcctcggg cgtgggcacg ccgaaggacg tcacgatcac
     6601 gccgcgcggg ttgaggcagc cgaccacgtg cttgatggca ccgtccttcc ccgccccatc
     6661 gcgcccctgc agcaccacca tgacgctgtt caccttggcg ccgtacatgg cgtcctgcag
     6721 gctgaagagt tcccgcccca gtgagtcgaa gcgcttctcg gccttctccc gcgtcatgcc
     6781 cttcggcgga tcggcaggga tgtcgtcgag gcggatcttt ccgcccggct tgtcgaacag
     6841 gagtcggggc atggctggcg aaattgcgga caggggcctg gggacgatct catgcgcaag
     6901 ctatcgcccg cacggccgcc cgtcaaccgg atcgcgtcgc ggaacgagcc gacccatcgg
     6961 cggaaccggc gggcgtgaag agcctgatgt cggccacgcg gctgcagggc gaggcttctc
     7021 gctgtgctcg gacagtcaat tgcgatagca ttgcacggaa gaacggcggc cgccgcgtgc
     7081 gaccgcaacc ctggacgcct tgtcgattcg agcgtgcagg acctgcccga ggctccacgc
     7141 gacacgatgc gcgagagtat cccgctgcaa cccctgcttg cacacgccgt cggcaactcc
     7201 gccgctgcga aggcagcaag acgccgcccc ccatgtgtcg cgaacggtcc ttgcgccggt
     7261 cgaatggcga tccccgccgg gcagccggcc ggtcggacag gaggatgaga agcgagatgc
     7321 agacttccaa gatcaccgac ctcgatattc atctctttcg cgaagggacc cacgcgcgtc
     7381 tctaccggtg cctgggctgc cagctcgatc caggcggcca gggcgggcga ttcgcggtgt
     7441 gggcgcccaa cgcccgcgca gtctcggtca tcggcgactg gaacggctgg aatgctggcg
     7501 ccgaccgcct gtcgccccgc gcggacggaa gcggcatctg ggagggcgag gtcgcgggcg
     7561 tgcgaagcgg ccaggcctac aagttccgca tcgagtcgca cgcgttgggc cacgtcgcgg
     7621 agaaggccga tcccttcgcc ttcctcgccg agtgcccgcc ggcgacggcc tcgcgcgcgt
     7681 ggtccctcga ctacgaatgg ggcgacgacg ggtggatggc ctcgcgcgcg cggcgcaacg
     7741 ccctcgacgc gccgatgtcg atctacgagg tgcatgccgg atcgtggcgg cgcaaggacg
     7801 gcgcatttct cggctaccgc gagctcgccc accagctcgc gcaatacgtg cgcgacgccg
     7861 gcttcaccca cgtcgagctc atgccggtga ccgagcaccc cttctacggt tcatggggct
     7921 accagacgac gggctacttc gcgccgaccg cgcgctatgg cacgccgcag gacttcatgt
     7981 acttcgtcga ccacctccac cggaacgaca tcggggtgat cctggactgg gttccgtcgc
     8041 acttcccggc cgatgcgcac gggctcggct tcttcgacgg cacctacctc tatgagcacg
     8101 ccgacccgaa gcagggcttt cacccggagt ggaactcgag catcttcaac tacggtcgca
     8161 acgaagtgcg cgcgttcctg ctttcctcgg ccctgttctg gctcgaccgg taccacctcg
     8221 acgggctgcg cgtggacgcc gtggcctcga tgctctacct cgactacgca cgccaggaag
     8281 gcgagtggat ccccaacgtc cacggcggca aggagaacct cgcggcgatc gatttcctgc
     8341 gcaccctcaa cacggccgtc taccgcgact accccgacac gatgagcatc gcggaagaat
     8401 ccaccgcctg gcccatggtg tcgcggcccg cctggctggg cggcctgggt ttcggcctga
     8461 agtggaacat gggctggatg cacgacaccc tcgcctacct gagcgaggac cccatcaacc
     8521 gcaagtacca ccacggcaag ctcaccttct cgctgatcta cgcgttccac gagaacttcg
     8581 tgctgcccct gtcgcacgac gaggtcgtcc acggcaaggg ctcgctcgtg aacaagatgc
     8641 ccggcgatac ctggcggcag ttcgccaacc tgcgcgcgct ctacggctac atgtgggcgc
     8701 accccggcaa gaagctcctc ttcatgggct gcgagttcgg ccagcggcgc gagtggatgc
     8761 acgacggcga gctcgaatgg tgggtgttgt ccctcgccga gcacgagggc gtgcggcgct
     8821 gggtcgccga cctcaaccgt ttctaccggg aggagcccgc gctctggcag gtggatttcg
     8881 atccgcacgg gttccagtgg atcgaggcga acgacgccga gaacagcgtc atctccttcc
     8941 tgcgccggcc gcgcggcgac ggcccggtcg tggtcgtcgc gtgcaacctc acgccggtgc
     9001 cgcgcgagaa ctactcgctg ggggtgccca tgcccgggcg ctgggccgag gcgctcaaca
     9061 gcgacgccac cctctacgga ggcgccggat ggggcaacct cggcggcctc gatgccgccg
     9121 cgacgggcgc gcacggccag cccttctcgc tcaccgtgac gctgccgccc ctgtccaccg
     9181 tgatcttcag gagcggggac cgtggttgag cgggcgagcg ccgccgagac gcagcccgcc
     9241 ggccgcgacg aggcggcgtc cgcggccgcg gacgcgctct ggtacaagga cgcgatcatc
     9301 taccagctgc acatcaaggc gttcttcgat tccaacgacg acggcgtcgg cgacttcccc
     9361 gggctcgcct ccaggctcga ctatgtgcag tcgctcggcg
//
LOCUS       DASUEV010000037         4798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_469983, whole genome shotgun sequence.
ACCESSION   DASUEV010000037 DASUEV010000000
VERSION     DASUEV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469983"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VFV55_01185"
     CDS             complement(<1..164)
                     /locus_tag="VFV55_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4942931.1"
                     /translation="MNLQQLRYAAEVARRGLNVSEAAAALHTSQPGVSKQLRALEEEL
                     GVDLFVRQGRR"
     gene            248..1921
                     /locus_tag="VFV55_01190"
     CDS             248..1921
                     /locus_tag="VFV55_01190"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX4942932.1"
                     /translation="MYRYDAIDSTLVAERVAQFRDQTRRHLAGELSEDDFRPLRLQNG
                     LYVQRHSPMLRVAIPYGLLSSTQLRKLARIARVYDRGYGHFTTRQNIQYNWPKLADVP
                     EMLAELAEVEMHAIQTSGNCIRNITTDHFAGVAPDEHVDPRALAEILRQWSTLHPEFA
                     YLPRKFKIALSGAVTDRAATLVHDIGLHAMRNGSGELGLRVIVGGGMGRTPIVGHVIR
                     EFLPWQEMLTYIEAILRVYNRHGRRDNLYKARIKILVKALGVEAFRAQVEAEWEHLRD
                     SDSTLTADELARVTAHFAPPAYEPPSDEDHALAFQLAADPAFARWVERNVHGHRRPGY
                     RAVTLSLKKTGVPPGDVTAEQMDLVADLADRYIFGELRVSHEQNLIFADVRERDLLAL
                     WKRARAAGLATPNIGLLTNIVCCPGGDFCALANAKSIPIAQAIQERFDDLDYLHDIGE
                     LDLNISGCMNSCGHHHIGHIGILGVDKNGAEWYQVSIGGEQGINASLGDVIGPSFAAA
                     EMPDVVERLVETYLAHRTEDERFVDTVHRIGIAPFKTRVYGGIREVVNA"
     gene            1914..2444
                     /locus_tag="VFV55_01195"
     CDS             1914..2444
                     /locus_tag="VFV55_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX4942933.1"
                     /translation="MPRHVIRHGQVFGDIWQVLVPGAGESPESIVLPEGPLAVTLPVW
                     KARREELLARSSPVGVRLEIADDPADIVADLPRLALVTVHFPRFTDGRGYSIAYLLRR
                     RHGYAGELRAVGDVLRDQLFYLARSGFDSFQLRDGTDPHAALPSLRDFSETYQSAADG
                     RVPRFRRGTASAEASP"
     gene            2441..3169
                     /locus_tag="VFV55_01200"
     CDS             2441..3169
                     /locus_tag="VFV55_01200"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX4942934.1"
                     /translation="MRLEMLLAQVRTTLERIEREFLPASLAVSFGAEDMVLVDLVARE
                     FPAIRLFTLDTGRLPKETLELKRAAEARYGIDIRVFSPKPENVAAYVNAHGENGFYES
                     LEKRKACCHVRKVEPLRRALAGHRAWITGLRRDQAPTRAALAAHEFDSAHGLHKFNPL
                     ADWTDADVWAYLRSHDVPVNALHARGYPSIGCEPCTRAVRPGEDPRAGRWWWEERDTK
                     ECGLHPVVPLVAAPISRANSEAAQ"
     gene            3166..4134
                     /gene="cysD"
                     /locus_tag="VFV55_01205"
     CDS             3166..4134
                     /gene="cysD"
                     /locus_tag="VFV55_01205"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX4942935.1"
                     /translation="MSAVLRDNDKGVRLLCTNYVQSNLTPLSHLDWLESEAIHILREV
                     VAETTNPALLFSGGKDSIVMLRLAEKAFRPDRFPFPLVHIDTEHNFPEVVAFRDARAA
                     ELGERLIVRSVGESIRQGRVRLRRPDESRNPHQTVTLLDTIREFGFDALFGGARRDEE
                     KARAKERIISHRDETGQWDPRNQRPELWSLFNAKLHPGEHLRVFPISNWTELDIWQYI
                     ARESLAVPRIYYAHDREVVERNGLLAPVTELTPPREGETVVTRRVRFRTVGDITCTCP
                     VASDADTVEKVIAETARATITERGATRMDDQTSDASMELRKREGYF"
     gene            4134..>4798
                     /locus_tag="VFV55_01210"
     CDS             4134..>4798
                     /locus_tag="VFV55_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4942936.1"
                     /translation="MGTRDAFREVTTLRFTTSGSVDDGKSTLIGRLLLDSKVLLEDQL
                     AAVARSSARNGGGDTLDLSLFTDGLTAEREQGITIDVAWRYFATPRRRFIIADTPGHE
                     QYTRNMVTGASTASLSIVLADAARGLTTQTRRHAAIASLLAIPHVVLAVNKMDLVGYP
                     EDLFGRIVDEYREFARHLQLDEVTAIPISALRGDNMLEPSDRTPWYHGPTLLGYLETV
                     DPG"
BASE COUNT          805 a         1753 c         1503 g          737 t
ORIGIN      
        1 cggcgcccct gccgcacgaa gaggtcgacg ccgagttcct cctcgagggc acgcaattgc
       61 ttggataccc ccggctgcga ggtatgcagc gccgccgccg cctcggagac gttgagccca
      121 cggcgggcca cctcggctgc gtaacgcaat tgctgcaggt tcatgccgac ctcatatatc
      181 cagtggttat cagcatatca ctatcaagta tttttcagga atatgccgga ccgctaggat
      241 tcgccgcatg taccgctatg acgccatcga cagcacgctc gtcgccgaac gggtcgccca
      301 gttccgcgac cagacgcgcc gccatctcgc cggggagctc tccgaggacg acttccggcc
      361 gctgcgcctg caaaacggcc tgtacgtgca gcgccattcg ccgatgctgc gcgtggcgat
      421 tccctacggc ctgctctcct cgacgcaact gcgcaagctc gcccgcatcg cccgcgtata
      481 cgacaggggc tacggccatt tcacgacgcg ccagaacatc cagtacaact ggccgaagct
      541 tgcggacgtg ccggagatgc tggccgagct ggccgaggtg gaaatgcacg ccatccagac
      601 gagcggcaac tgcatccgca acatcacgac cgaccatttc gcgggcgtgg ctcccgacga
      661 gcacgtcgac ccgcgcgccc tcgcggaaat ccttcgccag tggtcgaccc tccaccccga
      721 attcgcctac ctgccgcgca agttcaagat cgcgctgagc ggggccgtga ccgaccgcgc
      781 cgcgaccctc gtgcacgaca tcggcctgca tgccatgagg aacgggtcgg gcgagctggg
      841 cctgcgcgtg atcgtcggcg gcggcatggg ccgcaccccg atcgtcggcc atgtgatccg
      901 cgagttcctg ccctggcagg aaatgctcac ctacatcgaa gccatcctgc gcgtctacaa
      961 ccgccacggc cgccgcgaca acctgtacaa ggcgcgcatc aagatcctgg tgaaggcgct
     1021 cggcgtggaa gcgttccgcg cccaggtgga agccgagtgg gagcacctgc gcgattccga
     1081 ctccacgctc acggccgacg agctcgcgcg cgtcaccgcg catttcgcgc cgcccgccta
     1141 cgaaccgccc tccgacgagg accacgcgct cgccttccag ctcgccgccg atccggcctt
     1201 cgcgcgctgg gtcgagcgca acgtccacgg gcatcgccgg ccgggctacc gcgcggtcac
     1261 gctctcgctc aagaagaccg gcgtgccgcc cggcgacgtc acggccgaac aaatggatct
     1321 cgtggcggac ctcgccgatc gctacatctt cggcgagctg cgcgtctcgc acgagcagaa
     1381 ccttattttc gccgacgtgc gcgagcgcga cctcctcgcc ctgtggaagc gcgcgcgcgc
     1441 cgccggtctc gccacaccca acatcgggct gctcaccaac atcgtctgct gccccggcgg
     1501 cgacttctgc gcgctcgcca acgccaagtc gattccgatc gcgcaggcga tccaggagcg
     1561 cttcgacgac ctcgactacc tgcacgacat cggcgagctc gacctcaaca tttcgggctg
     1621 catgaattcc tgcggccatc accacatcgg ccacatcggc atcctcggcg tcgacaagaa
     1681 cggagcggag tggtaccagg tgtcgatcgg cggcgagcag ggcatcaacg ccagcctcgg
     1741 cgatgtgatc ggcccctcct tcgccgcagc ggagatgccg gatgtggtcg agcgcctggt
     1801 cgagacctac ctcgcccatc gcacggaaga cgagcgcttc gtggacaccg tgcaccgcat
     1861 cgggatcgcg ccgttcaaga cgcgcgtcta cggcggaatc cgggaggtgg tcaatgccta
     1921 ggcacgtgat ccgccatggc caggtcttcg gcgacatctg gcaggtgctg gtacccggcg
     1981 ccggggaaag ccccgagtcc atcgtcctgc cggaaggccc cctcgcggtg acgcttcccg
     2041 tgtggaaggc gcgccgcgaa gagctcctgg cgcgctcgtc cccggtcggg gttcgtctcg
     2101 aaatcgccga cgacccggcc gacatcgtgg ccgacctgcc gcgcctggca ctcgtgaccg
     2161 tgcatttccc ccggttcacg gacggacgcg gctactcgat cgcgtacctg ctgcgccgcc
     2221 gccacgggta cgcaggcgaa ttgcgcgccg tcggcgacgt gctgcgcgac cagctcttct
     2281 acctggcccg cagcggcttc gattcgttcc agttgcgcga cggcaccgac ccgcacgcgg
     2341 cactgccatc gctgcgcgac ttctcggaaa cctaccagtc ggccgccgac gggcgcgttc
     2401 cccgcttccg tcggggcacg gcatccgccg aggcttcgcc atgaggctcg agatgctgct
     2461 tgcccaggtc cgcaccaccc tcgagcgcat cgagagggaa ttcctgcccg cctcgctcgc
     2521 cgtgagcttc ggcgcggaag acatggtgct cgtcgacctg gtggcgcgcg aatttcccgc
     2581 gatccgcctg ttcacgctcg acacggggcg cttgccgaag gagacgctgg aactcaagcg
     2641 ggcggccgaa gcgcgctatg gcatcgacat ccgcgtgttc tcgcccaagc cggagaacgt
     2701 ggccgcctat gtgaacgcgc acggcgagaa cgggttctac gaaagcctcg aaaagcgcaa
     2761 ggcgtgctgc catgtccgca aggtggagcc gctgcgccgc gccctcgccg ggcaccgggc
     2821 ctggatcacc gggctgcgcc gcgatcaggc accgacgcgc gcggccctcg cggcgcacga
     2881 gttcgattcc gcccacggcc tgcacaagtt caacccgctg gccgactgga ccgatgccga
     2941 cgtgtgggcg tacctgcgca gccacgacgt gcccgtcaat gccctgcacg cgcgcggcta
     3001 cccgtccatc ggctgcgagc cctgcacccg cgccgtgcgt cccggcgagg acccgcgcgc
     3061 gggccgctgg tggtgggagg aacgcgatac gaaggaatgc ggcctgcatc cggtcgttcc
     3121 cctggtcgcg gcgccgattt cgcgcgccaa ttccgaggct gcccaatgag cgccgtcctc
     3181 agggacaacg acaaaggggt caggttactt tgcacaaatt atgtgcaaag taacctgacc
     3241 cctttgtctc acctcgactg gctggagtcc gaggcgatcc acatcctgcg cgaggtggtg
     3301 gcggaaacga cgaacccggc gctgctcttt tccggcggca aggactcgat cgtgatgctg
     3361 cgcctggcgg agaaggcttt ccgcccggac cgcttcccct tcccgctcgt gcacatcgac
     3421 acggagcaca actttcccga agtcgtggcc ttccgtgacg cccgcgcggc ggaactgggc
     3481 gagcgcctca tcgtgaggag cgtcggggaa tccatccgcc agggccgggt ccgcctgcgc
     3541 cgccccgacg agagccgcaa cccgcaccag acagttacgc ttctcgacac gatccgcgag
     3601 ttcggcttcg acgcgctgtt cggcggcgcc cggcgcgacg aggagaaggc gcgcgccaag
     3661 gaacgcatca tttcgcaccg cgacgagacc ggccaatggg acccgcgcaa ccagcgcccg
     3721 gagctgtgga gcctcttcaa cgccaaactc caccccggcg agcacctgcg cgtgttcccc
     3781 atttccaact ggaccgagct ggacatctgg cagtacatcg cgcgcgaatc gctcgcggtg
     3841 ccgcgcatct actacgccca cgaccgcgag gtggttgagc gcaacggcct gctcgccccg
     3901 gtgacggagc tcaccccgcc gcgcgagggc gaaaccgtcg tcacccgtcg cgtgcgcttc
     3961 cggaccgttg gcgacatcac ctgcacctgc cccgtcgcct cggacgccga taccgtcgag
     4021 aaggtcatcg cggagacggc gcgggcaacg atcaccgagc gcggcgccac gcgcatggac
     4081 gaccagacct cggacgcatc catggaactg cgcaagcgcg agggatactt ctgatgggca
     4141 cgcgcgacgc cttccgggaa gtcaccactt tgcgatttac gacgtcggga tcggtcgacg
     4201 acgggaaaag cacgctcatc ggccgcctgc tcctcgattc gaaggtgctg ctcgaggacc
     4261 agctcgcggc ggtcgcccgc agctccgcgc gcaatggcgg cggcgacacg ctcgacctgt
     4321 ccctcttcac cgacggcctc accgccgagc gcgagcaggg catcaccatc gacgtggcgt
     4381 ggcgctattt cgccacaccc cgccggcgct tcatcatcgc cgacaccccg ggccacgagc
     4441 agtacacgcg caacatggtg acgggcgcga gcacggcatc gctgtccatc gtgctggccg
     4501 atgccgcgcg cggcctcacg acgcagacac gacgccacgc cgcgatcgcc tcgctgctgg
     4561 cgattccgca cgtcgtgctc gcggtcaaca agatggacct ggtgggctac ccggaggacc
     4621 tgttcggccg gatcgtcgac gaataccgcg aattcgcccg ccacctccag ctggacgagg
     4681 tgaccgccat ccctatttcc gcgctccggg gcgacaacat gctggagccc agtgaccgga
     4741 cgccctggta tcacggaccg accctgctcg gttacctgga gaccgtcgat cccggcag
//
LOCUS       DASUEV010000038         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_126443, whole genome shotgun sequence.
ACCESSION   DASUEV010000038 DASUEV010000000
VERSION     DASUEV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126443"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFV55_01215"
     CDS             complement(<1..731)
                     /locus_tag="VFV55_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4942937.1"
                     /translation="MHTLDVEVAVIGAGTAGLAAYRAVRARGARAALIEGGPYGTTCA
                     RVGCMPSKLLIAAAEASHAIDHASGFGVHVTGTKRVDGREVMDRVRRERDRFVGFVLN
                     GVEEIPPGDRVVGRARFVDRNTLDVDGHTRVTARAIVIATGSRPAVPALLEGAGDRLV
                     VNDDVFSWDTLPRSVAVFGPGVIGLELGQALARLGVRVVVLGRGGRVGPLTDPVVRDA
                     ALRAIASELYLDPDAHVKRVTRVAQG"
     gene            complement(789..1538)
                     /locus_tag="VFV55_01220"
     CDS             complement(789..1538)
                     /locus_tag="VFV55_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX4942938.1"
                     /translation="MTPNKEGQRVPAVTFRTRQDNQWKDVTTGQIFAGKTVIVFALPG
                     AYTPTCSTTHLPRYNELAPTFAKLGVDEIVCLSVNDAFVMAEWQKDQDAPGITFIPDG
                     NGEFSEKMGMLVDKTNLGFGKRSWRYSMLVKDGVIVKQFIEPEKEGDPFEVSDADTML
                     KYLAPAVEAPAPAVIFSKPGCPHCARARALLEANGVAYEEVSLGKGVSFSTIRAVSGR
                     GTAPQVFIGGRHIGGADELAAYFAGGRKLAA"
     gene            1654..>2221
                     /locus_tag="VFV55_01225"
     CDS             1654..>2221
                     /locus_tag="VFV55_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4942939.1"
                     /translation="MTLTELRYVVALAQERHFGRAAQKCFVTQPTLSLALAKLEDELD
                     VRLFERNKSEVLVTPVGAEIVEQARRVLDEAGKIQSLAKGSQDQLSGALRLGVIPTIG
                     PYLLPELVRILRRSAPDMPLMIEETFTGNLVPMLRDGEVDAAIIALPFTVPGVKTRTL
                     YEEPFSVVVPEGHRWAKKRRVRPDELAGE"
BASE COUNT          364 a          727 c          777 g          353 t
ORIGIN      
        1 ccctgtgcca cgcgggtcac gcgcttcacg tgcgcatccg gatcgaggta gagctccgag
       61 gcgatggcgc gcagggcggc atcccgcacc acggggtcgg tgaggggacc gacgcgcccg
      121 ccgcggccca gcacgaccac gcgcacgcct aggcgggcga gggcctggcc cagttcgagt
      181 ccgatcacgc cggggccgaa gacggccacg gagcggggca gggtgtccca cgaaaacacg
      241 tcgtcgttca ccacgaggcg gtcgccggcg ccctcgagca gggccggcac ggcgggccgc
      301 gaaccggttg cgatgacgat cgcgcgcgcc gtgacgcgcg tgtgtccgtc gacgtcgagc
      361 gtgttgcggt ccacgaagcg ggctcggccg accacgcggt cgccaggggg aatttcctcg
      421 acgccgttca gcacgaagcc gacgaagcgg tcgcgttcgc gcctcacgcg gtccatcacc
      481 tcgcggccgt cgacccgctt ggttccggtg acgtgaacgc cgaatccgct ggcgtggtcg
      541 atcgcgtggg aggcttccgc cgcagcgatg aggagcttgg aaggcatgca gcccacgcgc
      601 gcgcaggtgg tcccgtacgg tccgccctcg atgagcgcgg cacgggcgcc gcgcgcccgc
      661 acggcacggt aggccgcaag gcccgcggtt ccggcgccga tgacggcaac ttcgacatcg
      721 agggtgtgca tcgcgaatct cctggaatga atggggccga ccccttggtc ggcccgcaat
      781 gccggaactt acgcggcgag tttccggccc ccggcgaagt aggccgcaag ctcgtcggca
      841 ccgccgatgt ggcggccccc gatgaagacc tggggtgcgg tgccgcggcc cgatacggcg
      901 cggatcgtgg agaagctcac gcccttgccg agcgatacct cttcgtaggc aacgccgttg
      961 gcctcgagga gcgccctggc gcgagcgcaa tgggggcagc cgggcttgct gaagatgacc
     1021 gccggcgcgg gcgcctcgac ggccggcgcg aggtacttga gcatcgtgtc ggcatcggat
     1081 acctcgaagg ggtcgccctc cttctccggc tcgatgaact gcttcacgat gacgccgtcc
     1141 ttcaccagca tcgaatagcg ccaggagcgc ttgccgaagc ccaggttcgt cttgtcgacg
     1201 agcatgccca tcttctccga gaactcgccg ttgccgtccg gaatgaacgt gatgccgggg
     1261 gcgtcctggt ccttctgcca ctcggccatc acgaaggcgt cgttcaccga caggcacacg
     1321 atctcgtcca cgccgagttt cgcgaacgtg ggcgcaagct cgttgtagcg cggcaggtgg
     1381 gtcgtcgagc aggtcggcgt gtaggcgccg ggcagggcga agacgatcac cgtcttgccc
     1441 gcgaagatct gcccggtagt cacgtccttc cactggttgt cctggcgggt gcggaacgtg
     1501 acggcgggaa cgcgctgtcc ttccttgttc ggggtcatgg tctggtctcc gggggttgat
     1561 tgataagcaa tggagtgaac tctaaagaat cgcgattgat tataccaatt aattgtggct
     1621 atacttccca tagtcaaccc aaatcgacgc cccatgaccc tcacggaact gcgctatgtc
     1681 gtcgccctgg cccaggaaag gcatttcggc cgggcggcgc agaaatgctt cgtcacccag
     1741 cccacgctct cgctggcgct ggccaagctc gaggacgagc tcgacgtgcg ccttttcgag
     1801 cgcaacaaga gcgaggtgct ggtcaccccg gtgggggcgg aaatcgtcga gcaggcccgg
     1861 cgggtgctgg acgaggcggg caagatccag tcgctcgcca agggaagcca ggaccagctg
     1921 tcgggggcgc tgcggcttgg cgtgatcccc acgatcgggc cctacctgct gcccgagctg
     1981 gtgcgcatcc tgcgccggag cgcaccggac atgccgctga tgatcgagga gaccttcacc
     2041 ggaaacctcg tcccgatgct gcgcgatggc gaagtcgacg cggccatcat cgcgcttccg
     2101 ttcacggttc ccggggtgaa gacgaggacg ctctacgagg agccgttcag cgtcgtggtg
     2161 cccgaggggc accggtgggc taagaagagg cgcgtgcgtc ccgacgaact cgccggcgag
     2221 a
//
LOCUS       DASUEV010000039         5923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_813739, whole genome shotgun sequence.
ACCESSION   DASUEV010000039 DASUEV010000000
VERSION     DASUEV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813739"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..614
                     /locus_tag="VFV55_01230"
     CDS             183..614
                     /locus_tag="VFV55_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942940.1"
                     /translation="MSRLMKMLLVSFTVASAAATVNADASEIRSVACTVTVDYLLNGV
                     LRAPYRKDFVVNPGEFFQDDFSTFTRFRAFEASTRLETGNTVVAIDYFNDVGVFESVD
                     LSTTLTLRDNDETISGSHSYFTSLGIAGEHTTNYTLACRRL"
     gene            complement(633..2561)
                     /locus_tag="VFV55_01235"
     CDS             complement(633..2561)
                     /locus_tag="VFV55_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX4942941.1"
                     /translation="MAPSRGALQVGLPAELVSHQPQGATAGLALLALGVVYGDIGTSP
                     LYAVKETFNPEHGIALNAANIVGGLSCIFWALMIIVTLKYVTLVLQADNRGEGGIVAL
                     LALATSSVGNRPRLGAVLVAIGVVGASLFYGDAVLTPAISVLSAVEGLEVGTDAFKPY
                     IVPIATGILIGLFLIQKHGTGVVGLLFGPICALWFVALAASGVWTILKNPGIVSALNP
                     VHAVRFATSHGYASFLVLGSVLLAVTGAEALYADMGHFGRRAIRVAWFGLVAPALVLN
                     YFGQGALLMADPAAVVNPFYLAFPPWALYPMVALATAATVIASQATISGAYSMTRQAM
                     QLGYLPRMTVLHTSARTMGQVYVPLVNWILLGAVGLAVIGFGSSSRLASAYGVSVMGT
                     MLATTFLTFFVVRYAWGYPLWLCVGATGFFLLVDASFFAAALHKVLEGGWFPILLGAA
                     IFGFMMTWRRGRRELLDQLQASSVPLLAFLESLFLSPPQRVPGTAVFLTSTPDATPHA
                     LLHSLKHYKALHERNVFMTVEFLDVPWMPFDQRVSCRSLGHECWRIAVRYGFMNKPDI
                     GAALELCGPFGLELDPGEVSYFLSREKIVPSGVAKGVLARVRDGIFAAMALNAGNITD
                     FFNIPTNRVVELGTRVTI"
     gene            2676..3752
                     /locus_tag="VFV55_01240"
     CDS             2676..3752
                     /locus_tag="VFV55_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX4942942.1"
                     /translation="MQKARPWIGYACAVASAAACTLVGYAMSPRFDIVNIAMVYLLAV
                     VLVALRFSRGPAIVSAIACVAAFDFMFVPPQGTFTVNDAQYLVTFAIILCVALVISYL
                     MERGQREADSRAALAIQAETERIRSTLLASISHDLRTPIAVMVGASSALTEGGEQLGT
                     AERSALAKSLYNQARDLAGQVDKVLQMTRLEAGAITLDRDWAAIAEIASTVLERLSER
                     LSDHRLLVEVADDLPLVRVDAGLIDQALANLLENAARYTPPGTVVRLRAKREGAEVVV
                     SVEDFGPGLDDQDIERVFAKFRRGTGEGAGGGVGLGLAICRAIVRLHGGRAWGERIPG
                     GGMAFRFSLPVEDAPLPPDEAEQA"
     gene            3754..4449
                     /locus_tag="VFV55_01245"
     CDS             3754..4449
                     /locus_tag="VFV55_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4942943.1"
                     /translation="MTESRATILVIEDDPEIRRFLRSSLGTEGYKVVESSTARRGVID
                     AATHKPDLAIVDLGLPDFDGVQVIGRIREWSPMPILVLSARIREHAKIEALDAGADDY
                     VTKPFGVGELLARVRVALRHRGRTSAGNPVLKLGSATIDLDARTAARDGRSVHLTPTE
                     FRLLNCLAKHLGMVVTHRQLLGEVWGPTHVSDTHYLRIYMKQLRDKLEADPLRPRHFM
                     TETGIGYRLVTDE"
     gene            complement(4403..5641)
                     /locus_tag="VFV55_01250"
     CDS             complement(4403..5641)
                     /locus_tag="VFV55_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4942944.1"
                     /translation="MTTEKRIPGVVAAGNVRIPPAIWALGGVSLLMDISSELIHSLLP
                     VFMVTTLGASALMVGLIEGAAEATALIVKVFSGVLSDFWGKRKPLALFGYGLGALSKP
                     LFALAASSGMVLAARLIDRVGKGIRGAPRDALVADIAPPEVRGAAFGLRQSLDTVGAF
                     VGPLLAIGLMLLWANDIRAVFWVAIIPGALSVALLFFGVQEPERPAGAKSVNPIRREH
                     LRRLGASYWWVVAIGAVFTLARFSEAFLVLRAQQGGLPLAYVPLVLVGMNVVYALAAY
                     PFGRLSDRMSHTKLLAIGLVVLIGAEVALAWSNHWAWVWCGISLWGLHLGITQGLLAT
                     MVADTAPDDLRGTAYGFFNLVSGIAMLIASVLAGFVWDRFGASLTFWVGGVFCALALL
                     AIGVRVIRPSRGDSRSRFRS"
     gene            complement(5638..>5923)
                     /locus_tag="VFV55_01255"
     CDS             complement(5638..>5923)
                     /locus_tag="VFV55_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942945.1"
                     /translation="AIRVSDKLTRREFERWMCRPREEGGRGFPKDVAQSRAILMQCLI
                     EGLAVRALRDPDLDARTLKGALEPLFLLLGLAQDDPPARRIRAGTSGPSR"
BASE COUNT         1112 a         2023 c         1917 g          871 t
ORIGIN      
        1 accccaggat cgtcgcgacg gtcagggcgc tggcggcaag gtagtacggg gccgaagcat
       61 catcgcgcga tccggccgtt gtatttgcgc accccggggc gactctgttg gccaggggag
      121 gagatgcttc tgccgggagg gccttccccg tctgccaatt gaccctgggg aggggaaaaa
      181 tcatgtccag actgatgaag atgctgctgg tgtcgttcac ggtggccagc gctgccgcaa
      241 cggtcaacgc ggatgcctcg gagatccgca gcgtggcctg caccgtgacc gtggattacc
      301 tgctgaacgg cgtcttgagg gcaccctacc ggaaggactt cgtggtcaat cccggcgaat
      361 tcttccagga cgacttttcc acgttcacgc gcttccgggc attcgaggca tcgacacgtc
      421 tcgagacggg caacaccgtc gtggcgatcg attatttcaa cgacgtgggc gtcttcgagt
      481 ccgtcgacct gagcacgacg ctgaccctgc gcgacaacga cgaaaccatt tcgggaagcc
      541 acagctactt cacgagcctg ggcatcgcgg gggaacatac caccaactac acgcttgcct
      601 gcaggcgcct gtagcggcac tcctgcccgc cctcagatag tcaccctggt tcccagttcg
      661 acgacgcgat ttgtcgggat attgaagaaa tccgtgatgt tccccgcgtt cagggccatt
      721 gccgcaaaaa tgccgtcgcg cacccgcgcc agcacgccct tggcgacacc ggagggcacg
      781 atcttctcgc gggaaaggaa gtaggacacc tctcccggat cgagttccag gccgaagggc
      841 ccgcacaact ccagtgcggc gccgatgtcc ggcttgttca tgaatccgta tcggacggcg
      901 atgcgccagc attcatggcc caggctcctg cagctcaccc gctggtcgaa tggcatccag
      961 ggcacgtcga ggaactcgac ggtcatgaat acgttgcgct catgcaaggc cttgtagtgc
     1021 ttgaggctgt gcagcagcgc gtgcggcgtg gcatccgggg tcgaagtgag gaacacggcc
     1081 gtacccggta cgcgctgcgg tggcgaaagg aacagcgatt cgaggaacgc cagcaacggg
     1141 accgaggacg cctggagctg gtcgagcaac tcccttcgtc cacgccgcca cgtcatcatg
     1201 aacccgaata ttgccgcgcc gaggaggatc gggaaccagc cgccttccag caccttgtgc
     1261 aaggccgcgg caaagaaact cgcatccacg agcaggaaga aacccgttgc ccccacgcac
     1321 aaccaaagcg ggtagcccca cgcatagcgg accacgaaga acgtgaggaa agtcgtcgcc
     1381 agcatcgtgc ccatgacgga cacgccgtag gcggacgcaa gccggctgga agagccgaag
     1441 ccgatgaccg ccagcccgac ggcgcccagc aggatccagt tgaccagggg cacgtagacc
     1501 tgtcccatcg tccttgcgga ggtgtgcagc accgtcatgc gcggcaggta ccccagttgc
     1561 atcgcctgac gcgtcatgga atacgcgccg gagatcgtcg cctgcgaggc gatcaccgtc
     1621 gccgccgtgg cgagcgccac catcgggtag agcgcccacg gcggaaaagc caggtagaac
     1681 ggattgacca ccgccgccgg atcggccatg aggagcgctc cctggccgaa atagttcagg
     1741 accagggccg gcgccacgag gccgaaccag gccacgcgaa tcgcacgccg gccgaaatga
     1801 cccatgtcgg cgtacagggc ctcggccccg gtgacggcca gaaggacgga gcccaggacg
     1861 agaaacgagg cgtaaccatg cgacgtggcg aagcggaccg catggacggg gttcagggcc
     1921 gagacgatcc cgggattctt caggatagtc cacacgccgg aggcggcgag cgcgacgaac
     1981 cacagcgcgc agatcggccc aaacaatagg cccacgacgc ccgtgccgtg cttctggatg
     2041 agaaagagcc cgatcaggat gcccgtcgcg atggggacga tgtagggctt gaacgcatcg
     2101 gttccgacct cgaggccctc cacggcggac agcacggaaa tggccggcgt cagcacggcg
     2161 tcaccataga atagcgaggc accgaccacg ccgatggcaa cgagcaccgc gcccaggcgc
     2221 ggcctgttgc ccaccgagga cgtggcgagc gcgagcaggg ccacgatgcc cccctcgccc
     2281 cggttatccg cctgcaggac cagcgtcacg tacttgagcg tgacgatgat catcagcgcc
     2341 cagaagatgc aggacagacc gccgacgatg ttcgccgcat tgagcgcgat gccgtgctcc
     2401 ggattgaagg tttccttcac cgcgtagagc gggctcgtgc cgatgtcgcc ataaacgacg
     2461 ccgagcgcga gcagggcgag ccccgccgtg gcgccttgcg gttggtgcga gaccagctct
     2521 gccggcaggc caacctgcag cgcgccgcgc gatggcgcca agccgggatc ggaatcattc
     2581 atcggcgaaa gtgtaggcgc catcaccagc ctcgcacgga gccggggtcg tcttttaacg
     2641 gctcctttac ggcggccatg cgctagagtg gccgcatgca gaaggcacga ccctggatcg
     2701 gctatgcgtg cgccgtcgca tcggcggcgg cgtgcacgct ggtcggctac gccatgtcac
     2761 cgcggttcga catcgtgaac atcgccatgg tctacctgct ggccgtcgtc ctggttgccc
     2821 tgcggttcag ccgcggaccg gcgatcgtga gcgcgattgc ctgcgtcgcg gcgttcgact
     2881 tcatgttcgt gccaccgcag ggcacgttca cggtgaatga cgcccagtac ctggtcacct
     2941 tcgcgatcat tctctgcgtc gcgctcgtga tttcctacct gatggaacgc gggcaacgcg
     3001 aagccgattc acgcgccgcg ctcgccatcc aggccgagac ggaacgcatc cgcagcacgc
     3061 ttctcgcatc catatcgcac gacctgcgaa cgcccatcgc cgtgatggtc ggcgcctcct
     3121 cggcgctgac cgaaggcggg gagcaattgg gcacagcgga acgcagtgcc ctcgcgaaga
     3181 gcctctacaa ccaggcccgg gaccttgccg ggcaggtgga caaggttctg caaatgacac
     3241 gcctcgaagc gggtgcgata acgctcgatc gcgattgggc ggccatcgcc gagatcgcga
     3301 gcacggtcct cgagcgcctg tcggagcgcc tgtccgatca ccgcctcctg gtcgaagtcg
     3361 ccgacgacct gccgctcgtg cgcgtcgatg cgggactcat cgaccaggcg ctggccaacc
     3421 tgctcgagaa cgcggcccgc tacacgccgc cgggaacggt cgtgcgcctg cgcgcgaaac
     3481 gcgaaggggc cgaggtcgtc gtgtccgtcg aggatttcgg gcccggcctg gacgaccagg
     3541 atatcgaacg cgtgttcgcc aagttccgcc gtggcacggg cgaaggcgcc ggcggtggcg
     3601 tcggcctggg cctcgccata tgccgggcca tcgtgcgcct tcacggcggg cgggcctggg
     3661 gggagcggat accggggggt ggaatggcgt ttcgattcag ccttcccgtc gaggacgcgc
     3721 cgcttcctcc cgacgaggcg gagcaggcat gacgtgacgg agagccgcgc cacgatcctc
     3781 gtcatcgagg acgatcccga gatccgccgc ttcctgcgct cgtcgctcgg caccgaaggc
     3841 tacaaggtgg tcgaatcctc caccgcgcgg cgcggtgtca tcgacgccgc cacgcacaag
     3901 ccggacctcg ccatcgtgga cctcggactc ccggacttcg acggcgtgca ggtgatcggc
     3961 cggatccgcg agtggtcgcc gatgccgatt ctcgtgctgt cggcgcgcat ccgggagcac
     4021 gccaagatcg aagccctcga cgcgggcgcc gacgattacg tcaccaagcc gtttggcgtc
     4081 ggcgagttgc tggcgcgcgt gcgcgtcgca ctgcgccatc gtggccgcac gagtgccggc
     4141 aacccggtgc tgaagctggg aagcgcaacc atcgatctcg atgcacgcac ggcagccagg
     4201 gacggccgca gtgtccacct cacgcccacc gagttccgcc tcctcaactg cctcgcgaag
     4261 cacctcggca tggtcgtgac gcaccggcaa ctgcttggcg aggtgtgggg tccgacccac
     4321 gtctcggaca cgcactacct gcgcatctac atgaagcagc tgcgcgacaa gctggaggcg
     4381 gacccgttgc ggccgcgcca tttcatgacc gaaaccggga tcggctatcg cctcgtgacg
     4441 gacgaatgac ccgcaccccg atggcgagga gggccagcgc gcagaacacg ccaccgaccc
     4501 agaacgtgag cgaggcgccg aagcggtccc acacgaagcc cgccagcacg ctggcgatca
     4561 gcatcgcgat ccccgagacc aggttgaaga agccgtacgc ggtgccccgc agatcgtccg
     4621 gcgccgtgtc ggcgaccatc gtcgcgagca acccctgggt gatgcccagg tgcaggcccc
     4681 acagcgaaat tccgcaccat acccacgccc agtggttgct ccaggcgagc gcgacttccg
     4741 cgccgatcag cacgacgagc ccgatcgcca gcagtttcgt gtggctcatg cggtccgaaa
     4801 gcctgccgaa gggatacgcc gccagcgcgt aaaccacgtt catgccgacc agcaccagcg
     4861 gcacgtaggc gaggggcagc cctccctgct gcgcgcgcag gaccaggaat gcctcgctga
     4921 accgcgccag ggtgaacacc gcgccgatcg ccaccaccca ccaatacgac gccccgagcc
     4981 ggcgcaggtg ttcacgccgg atcggattga ccgatttcgc tcccgcgggt ctttcgggct
     5041 cctgcacgcc gaagaacaac agggcgacgg aaagggctcc cgggatgatc gcgacccaga
     5101 acaccgcgcg gatgtcgttc gcccacagca acatcaggcc gatcgccagc aacgggccga
     5161 cgaaggcgcc gacggtgtcc agcgattgcc gcaggccgaa tgccgccccg cgcacttccg
     5221 gcggagcgat gtcggcgacc aatgcatcgc gcggcgcccc gcgaatcccc ttgcccacgc
     5281 ggtctatcag gcgtgccgcg aggaccatgc cggaactcgc ggccagcgcg aacaagggct
     5341 tggacagggc ccccaggccg taaccgaaca gggcaagcgg cttgcgcttc ccccagaaat
     5401 cgctgagcac ccccgagaac accttcacga tcagcgccgt cgcctccgcc gcgccttcga
     5461 tcagcccgac catcagggcg ctcgcgccga gagtcgtcac catgaacacg ggcagcaggc
     5521 tgtggatgag ctcggaggaa atgtccatca ggaggctcac gccgccgagc gcccagatcg
     5581 ccggcgggat acggacattc ccggcggcca cgacgccggg gatccgtttc tcggtcgtca
     5641 tcgcgacggg ccggacgtcc ctgccctgat tctccgcgcc ggcggatcgt cctgcgcaag
     5701 ccccaggaga agaaacagcg gttcgagcgc acccttgagc gttcgtgcgt cgaggtcggg
     5761 atcgcgcaat gcccgcaccg caagcccttc gatcaggcac tgcatcagga tcgcgcggga
     5821 ctgggcgacg tccttcggga atccccgccc gccctcctcc ctgggccggc acatccagcg
     5881 ttcgaattcc ctgcgcgtga gcttgtccga cacgcggatc gcc
//
LOCUS       DASUEV010000040        18276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1609621, whole genome shotgun sequence.
ACCESSION   DASUEV010000040 DASUEV010000000
VERSION     DASUEV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 18276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18276
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609621"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..851)
                     /locus_tag="VFV55_01260"
     CDS             complement(168..851)
                     /locus_tag="VFV55_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4942946.1"
                     /translation="MELLSHIELATGADPAGTVIWMHGLGADGWDFVPLVRELGLPEE
                     LPLRFIFPHAPVRPVTINNGYAMRAWYDIAMADIGRLPDEAGIRQSQAQVEAFLARER
                     SRGIAASRIVVAGFSQGGAIALHTGLRHAESLAGILALSTYLPLAETLARESSHANDR
                     IPILMAHGTQDPVVPIALAESSRAMLTARGLSPEWHTYPMPHSVCAEEVAAISRWLVA
                     RYTSPILAA"
     gene            complement(874..1470)
                     /gene="mog"
                     /locus_tag="VFV55_01265"
     CDS             complement(874..1470)
                     /gene="mog"
                     /locus_tag="VFV55_01265"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX4942947.1"
                     /translation="MSTHDPVTIGLVATSDRASQGVYRDEGIPALEAWLAGALLNPVR
                     FERRLIPDERATIEATLVELVDRAGCHLVITTGGTGPAPRDVTPEATLAVADREMPGF
                     GEQMRRISLEFVPTAILSRQVAVIRRGALIINLPGQPKSIRETLEGAKDADGRPRVAG
                     IFAAVPYCIDLIGGPYLETRDEVVKAFRPKSAIRPPKP"
     gene            complement(1467..1970)
                     /gene="yjgA"
                     /locus_tag="VFV55_01270"
     CDS             complement(1467..1970)
                     /gene="yjgA"
                     /locus_tag="VFV55_01270"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX4942948.1"
                     /translation="MNDENLISKSRRKRQARELQDIGVTLTRLRADQLSRIEMPDDLR
                     EAVVECRKMTKHEAIRRQLQLIGRIMRNLDAAPIAAQLEALHAPSLHQTALFHRVEKW
                     RDDLLADPGAVERFALEYPGADPQRLRSLAAAALEERLAERPPKRYRELFRAINAVLE
                     EHAKRTP"
     gene            2072..3427
                     /gene="pmbA"
                     /locus_tag="VFV55_01275"
     CDS             2072..3427
                     /gene="pmbA"
                     /locus_tag="VFV55_01275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX4942949.1"
                     /translation="MPDTPAVPALPLDRAALESAALRALDLARSRGASAAEAESSASV
                     GQSVTVRLGEVETVEYNRDKALAVTVYFGARRGNASTSDLSGDSIVRTVDAACAIARH
                     TAPDEAAGLPDADRLYRGEAGGLDLYHPWNLSVEDAIVLARETEDAARAVDARITNSE
                     GASVSSWDADFVFANSLGFIGGFPSSRASIGCGVLAAQGESMQRDYWYTSERDPARLE
                     AAQAVGRRAGERAVRRLGARRVATVEVPVLFEANIAGSLIGHFVSAASGSSLYRRSSF
                     LIDAAGKPVFSPIVNLDEEPHIPGGMASGWFDGEGVATSARRVVDAGTLTGYFLSTYS
                     ARKLGLQTTGNAGGCHNLLLQPGREDFAGLVRAMGRGLVVTELMGQGVNPVTGDYSRG
                     AVGFWVEGGEICYPVEEVTIAGNLPEMYRGIIAVGSDVLVRGARSSGSILVERMTLAG
                     H"
     gene            3528..4628
                     /locus_tag="VFV55_01280"
     CDS             3528..4628
                     /locus_tag="VFV55_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX4942950.1"
                     /translation="MQRRKFLTSAATGLAAGAAMMPTLSRAQAAALPEVKWRLASSFP
                     KSLDTLFGTAEFMSKRVAACTGGKFQIQVFAAGELVPGLQVLDAVQNGTVQACHTAPY
                     YFWGKDPAFALDCALPFGLNARQMNAWQIFGGGLELLREFYKGYNVHNIPCGNTGAQM
                     GGWFRKEIKTVADLKGLKFRISGYAGKIIEKQGGVPQQIAGGDIYPALEKGTIDAAEW
                     VGPYDDEKLGFNKVAKYYYYPGWWEGGPQLSAYFNVKEWDKLPKEYQNIVEAACGEAN
                     SWMLAKYDAQNPAALKRLVASGTQLRAFSREIMVASYKATLEVNKESSEKSPAFKKIY
                     ESMVKFRDEELLWFRVADKGYDDFMHGIGSIK"
     gene            complement(4644..6470)
                     /locus_tag="VFV55_01285"
     CDS             complement(4644..6470)
                     /locus_tag="VFV55_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX4942951.1"
                     /translation="MMEFLVHNMAPIMFAALVVFLLLGYPVAFALAANGIIFGLVGIE
                     LGLLQPALFQALPERVWGVMSNDTLLAVPFFTFMGLILERSGMAEDLLDTVGQLFGPL
                     RGGLAFAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRFASGVIVASGTLA
                     QIIPPSLVLIIMADQLGKSVGDMYAGAFIPGIALTGLYALYAIGVTFVKPAWAPALPP
                     DARRLKEPDGSSGTPSFVVLATVSTLVAVAFDRFYYKATYVEAMLDERLVLAAAAGAG
                     FAFAAAIANRLLRLGLLSRLAEQVVFVLIPPLALIFLVLGTIFLGWATPTEGGAMGAT
                     GALVMAIARRRLPFKLLRQAMDATAKLSTFVLFILIGARVFSLTFYGVNGHVWVEELL
                     VGLPGGQLGFLIVVNLLIFVLAFFLDFFELSFIVVPLLGPVAEKLGIDLIWFGVLLGV
                     NMQTSFMHPPFGFALFYLRSVAPAKEYLDRVTGKLTAPVTTGQIYWGAIPFVLIQLVM
                     VALVIVFPQMVMVYKSDQSTVDPAKVKFEIPEMDQGNVPEDILRDLGGKGPPATAPEK
                     EDPAKAIERELKGQASPPTPSKEKTPEDKAAEEIEKALRGGK"
     gene            complement(6467..7057)
                     /locus_tag="VFV55_01290"
     CDS             complement(6467..7057)
                     /locus_tag="VFV55_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX4942952.1"
                     /translation="MQALLKLSRGIDALNERVGRTVYWLILAAVLISAGNAIIRKVFN
                     YSSNAFLEAQWYLFSAVFMLCAGYTLLRNEHVRIDVIAGRLSRRTQAWIDVLGTLFFL
                     LPMALIFIYLSWPVFVRTWVHDEMSGSAGGLAIWPARLIVPIGFLLLALQAVSELVKR
                     IGFLRGVAPDPIDILHRRSAELELAEDIRKLAEGRS"
     gene            7246..8520
                     /gene="glyA"
                     /locus_tag="VFV55_01295"
     CDS             7246..8520
                     /gene="glyA"
                     /locus_tag="VFV55_01295"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX4942953.1"
                     /translation="MFPDTHTIAAVDPEIWQAIRDEYRRQEDGIELIASENYASPAVM
                     AAQGTILTNKYAEGYPGKRYYGGCEFVDVAESLAIERVKKLFGAEYANVQPHSGSQAN
                     QAVYMAMLRPGDTILGMSLAHGGHLTHGAAVNLSGKLYHSVAYGLSEAEEIDYDQVRD
                     LAREFNPRMIVAGASAYALRADWQHFRDIADEVGAMLMVDMAHYAGLVAAGLYPSPVG
                     IADFVTSTTHKTLRGPRGGIILAAAEHETALNSAIFPTIQGGPLMHVIAAKAVAFKEA
                     LEPAFKSYQAQVLANAQRMATTLAQRGLRIVSGRTESHLFLVDLRAKKVTGKDAEAAL
                     GKAHITVNKNAIPNDPEKPFVTSGIRVGSPAMTTRGFADAEAEQVAHLIADALESHAD
                     DAGLAGVRANVNALCAKFPVYGPAMRATYGLA"
     gene            8542..8994
                     /gene="nrdR"
                     /locus_tag="VFV55_01300"
     CDS             8542..8994
                     /gene="nrdR"
                     /locus_tag="VFV55_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX4942954.1"
                     /translation="MKCPFCSAEDTQVIDSRVSEEGDSIRRRRRCQVCAKRFTTYETA
                     ELRMPQIVKGNGQREDFSTAKLRTGFQRALHKRPVTTELVDAAITRIEQKLLALGERE
                     VDSRRIGEMVMEELRKLDKVAYIRFASVYRSFSDVDDFRDAIREVRDK"
     gene            8996..10087
                     /gene="ribD"
                     /locus_tag="VFV55_01305"
     CDS             8996..10087
                     /gene="ribD"
                     /locus_tag="VFV55_01305"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX4942955.1"
                     /translation="MSFPAEDHALMARALALAALGRDTATPNPNVGCVIAKAGRVIGE
                     GWHARAGEPHAEAMALAACTESPEGATAYVTLEPCSHHGRTPPCADALLAARVARVVA
                     ALEDPNPLVTGQGFAKLRAGGIRVESGLLAAEAKEVHLGFLARMSRGRPWMRIKSAAS
                     LDGRIALANGESRWITGEAARRDVHALRARSCAMLTGIGTVLRDDPELTVRHVPCSRQ
                     PRRVVIDSRLELPAAARILSGEPPLVLTVSEDRGRRAALEALGAEVVVVPAEGAKTDL
                     GAVARLLADRGFNEVTVETGAKLNGSLLRAGIVDEIVLYLAPLLLGDAAQGLFALPVF
                     GQLGEALRPRIVDIRAVGADLRITARLET"
     gene            10089..10712
                     /locus_tag="VFV55_01310"
     CDS             10089..10712
                     /locus_tag="VFV55_01310"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX4942956.1"
                     /translation="MFTGIVTAIGRIASARRHGDGLRLRIDAPGFGMDDVALGDSIAV
                     QGVCHTVVAKDASGFDVDTSAATLAVTTGLAEGREVNLEKSLTLADRLGGHLVSGHVD
                     GVGTVTAFDDLGGSWRLEIRMPQELSRYVARKGSIAVDGVSLTVNSVTSDRFEVNIIP
                     HTRSATTLRRLAAGSPVNLEVDMTARYLERLLGAREQGIPPGSLPES"
     gene            10809..11321
                     /locus_tag="VFV55_01315"
     CDS             10809..11321
                     /locus_tag="VFV55_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942957.1"
                     /translation="MVRSELVPMPGKAGHRACIGFLAAIACLALTVASARQLPVPWPV
                     PPFASSPHRLAAAPAWDCWIDPGEGGGIRCIADRDVPLPLPDEFTDDDEAAEVLLELL
                     HDYLHRAMPQRASQLVRERYYLLRRDDLWTIRLSSPPFETSWSDERPQLLVRSLLCRG
                     APGCPIRFHH"
     gene            11395..12513
                     /gene="ribBA"
                     /locus_tag="VFV55_01320"
     CDS             11395..12513
                     /gene="ribBA"
                     /locus_tag="VFV55_01320"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX4942958.1"
                     /translation="MNISPTAEIIDDIRHGRMVVLVDAEDRENEGDLVLAADFVTPET
                     INFMATHARGLVCLTLTEERCRQLALPLMVSDNRSPHGTAFTVSIEAAVGVSTGISAA
                     DRARTVRMAVAKHAKPADIVQPGHIFPVMAQNGGVLVRAGHTEAGCDLAELAGLTPAA
                     VICEVMKPDGEMARLPDLVEFAARHGLKLGTIADLIRYRSENERLIDRVADKTIATPY
                     GPFRLCVYHDSSAGELHYALVKGEPSPSRPVLVRVHEPFHSIDMFDFDSSRHAYSVQD
                     SMRIISHHKEGVIVLLRRHEAPEEVLERFSMHAAPARGVRKWDPKLHGIGAQILRDLG
                     VGRMRVLARPKRIPSMAGFGLEVVEYVSPDDASQVKAI"
     gene            12510..12980
                     /gene="ribH"
                     /locus_tag="VFV55_01325"
     CDS             12510..12980
                     /gene="ribH"
                     /locus_tag="VFV55_01325"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX4942959.1"
                     /translation="MTAYTSELKGEGLTVAIVWSRFNEDIVRELLTACDRELVNLGVA
                     TGDIDVVSVPGALEIPLVLQALALRTRGFTRRYDALVALGAVIRGETYHFEVVSNESA
                     RGILDVQLETGVPVANAVLTTSTEEQARERAAEKGAEAARVAVEMANLLKAVSG"
     gene            12977..13477
                     /gene="nusB"
                     /locus_tag="VFV55_01330"
     CDS             12977..13477
                     /gene="nusB"
                     /locus_tag="VFV55_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX4942960.1"
                     /translation="MRTPRRRAREFALQGLYQWLLTGAPAGEIARNLAELEGYGAADE
                     AFLKAELAGVIGEAEGLQALLAPLADRKWAEVSPVEKSILLIGAWEIAHVPEMPYRVA
                     INEAIELAKRFGGIDGHKYVNGILDRVAAAVRPEEIAAGRAADRKPRQASRAPAGATP
                     PKKAEA"
     gene            13474..14445
                     /gene="thiL"
                     /locus_tag="VFV55_01335"
     CDS             13474..14445
                     /gene="thiL"
                     /locus_tag="VFV55_01335"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX4942961.1"
                     /translation="MIEKSPEFDLIARHFTRPARNAVLGVGDDCALVDVSNGMDLAVS
                     TDTMVSGTHFFPDVDPETLGHKALAVNLSDMAAMGAMPYWAMLALTVPDVNHEWLAKF
                     AKGFFDLAEEFGVSLIGGDTTKGPLTMTVTIMGEVPAGAALRRGGAKAGHDIWVSGNL
                     GDAALAVAHRRGLLKLSEGEYMEAVMRLYEPAPRVKLGQALRGLATSAIDISDGLLAD
                     LDHICTLSKVGATVEVERLPLSAIGAKHIDSPAGLTAIVAGGDDYELCFTAPATARDS
                     IEDLTEVLGVPLTRIGEIRKGKGVSLVGADGKPIAVDGRGFDHFTAS"
     gene            14417..14932
                     /locus_tag="VFV55_01340"
     CDS             14417..14932
                     /locus_tag="VFV55_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX4942962.1"
                     /translation="MDSTTSRRPDARFLVAHPAHFIALGFGAGLSPRAPGTVGTLVAL
                     ALYWILAIALPPLAIAFLAVPLFFVGVWACGVAGRNLGVEDHGAFVWDEIVAFLPLAA
                     LASASPWLQALAFALFRLFDIWKPFPIRQVESRVKGGLGVMLDDLLASCYAYLVLVIL
                     VVAVHRFPPAP"
     gene            14929..15681
                     /gene="ugpQ"
                     /locus_tag="VFV55_01345"
     CDS             14929..15681
                     /gene="ugpQ"
                     /locus_tag="VFV55_01345"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX4942963.1"
                     /translation="MRKTWPYPQLVAHRGGGLLAPENTLAAIRLGQSLGFRAAEFDVK
                     LTRDDVAFLLHDSTLDRTTSGSGAAGDRNWEELAALDAGGWHSETFSGEPIARFDEAA
                     RLLRSAGTIAIVEIKPVPGTERATGLAVAAQAQAHWRDAEVPPLLSSFSRESLAAARE
                     SAPLLPRGLIVEAPTDADFASLDSLGAVSLHCGRKYATPELFDRAHAAGYRVLVWTVN
                     DPPEAKRLLAWGADGIITDNLRQFATRFPDRL"
     gene            15846..16649
                     /locus_tag="VFV55_01350"
     CDS             15846..16649
                     /locus_tag="VFV55_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX4942964.1"
                     /translation="MARIAVFNQKGGVGKTTTALNLAAAIAARGGRPLAIDLDPQAHF
                     SAIAGALPRTAEGSIYALYRDARPLRELFVDSALGWVVIPAHLELAKVDTQFGKGPNI
                     LNRLRAGLAREGLGRTGSVLIDCSPMLGVLSLSAIFAADRVLVPVSTDYLAVNGAMQV
                     DRTLNALQRVVGRRIERRYVITRFDGRRRMSWDILATLKARFGADLAESRISENVSIA
                     ESPYNRRDVFAHAPASRGAADYRAMFEELESSGFLDEFPVAGGMLAAVA"
     gene            complement(16657..16899)
                     /locus_tag="VFV55_01355"
     CDS             complement(16657..16899)
                     /locus_tag="VFV55_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942965.1"
                     /translation="MVLVEMKKFGFRIRTRGGSVVDNLTIQGRDRDEAERKLRQVYHH
                     CVILEARELRESAPVDGSDFEGVINLIADEGSRDGG"
     gene            complement(16931..>18276)
                     /locus_tag="VFV55_01360"
     CDS             complement(16931..>18276)
                     /locus_tag="VFV55_01360"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX4942966.1"
                     /translation="AARARGARAVLIEGGPYGTTCARVGCMPSKLLIAAAEASHAIDH
                     AGGFGVHVTGTKRVDGREVMDRVKRERDRFVGFVLNGVEEIPPADRVVGRARFVDRNT
                     LDVDGHTRVTARAIVIATGSRPTVPALLEGAGDRLVVNDDVFSWDTLPRSVAVFGPGV
                     IGLELGQALARLGVRVVVLGRGGRVGPLTDPVVRDAALRAIASELYLDPDAHVKRVTR
                     VAQGVEVEFTNLESAACVETFDYVLAATGRRPNVDGLGLESLGLELDKAGVPRFDTHT
                     LRVGDTNLFIAGDASNYIPVLHEAADEGRIAGDNAAGFPAVQPGLRRAPLAVVFTDPQ
                     IAIVGGGYAKLVPGSFEVGEVSFENQGRARVIRMNRGHLRVYADRATGRFLGAEMAGP
                     AAEHIGHLLAWALANRMTVASMLEMPFYHPVVEEGLRTALRDLAAKLRSPQAIAA"
BASE COUNT         2925 a         6220 c         6304 g         2827 t
ORIGIN      
        1 cgagattgca cgcaacgcaa ttgcatcgca gctcggtcaa cgcggtgccg aattacgcag
       61 cgcattcgaa tcgcggctcc atccccggcc caccctcata tccatccccg gccctgaacg
      121 cattccactt tccgggcgag ccgtgccttg cgtcgcggtg cggcgtttca ggcagcgagg
      181 atcgggctcg tgtagcgcgc cacgagccac cgcgaaatcg ccgccacctc ctcggcgcag
      241 acggagtgcg gcatcggata ggtgtgccac tcgggcgaga gcccgcgcgc ggtgagcatc
      301 gcgcgcgacg attccgcgag tgcgatcggc acgaccgggt cctgcgtgcc gtgcgccatc
      361 aatatgggga tgcgatcatt ggcatgcgac gactcgcgcg ccagtgtctc ggcgagcggc
      421 aggtacgtgg acagggcgag gatgcccgcc agcgactcgg cgtgccgcag gcccgtgtgc
      481 agggcgatcg ccccgccttg cgagaacccc gcgacgacga tccggcttgc cgcgatcccg
      541 cgcgatcgtt cccgcgccag gaacgcctcc acctgcgcct gcgactgtcg gatcccggct
      601 tcgtcgggga gccgaccgat gtcggccatc gcgatgtcgt accacgcccg catggcgtag
      661 ccgttgttga tcgtgaccgg gcgcacgggg gcgtgcggaa agatgaatcg aagcggcagt
      721 tcctccggca ggccgagctc gcgcaccagg ggaacgaagt cccatccgtc cgcgcccagc
      781 ccgtgcatcc agatcacggt gcccgcggga tcggcgccgg tggcgagttc tatgtgcgac
      841 agcagttcca tttctggtcc tcggggaaaa tggtcagggc ttcggggggc ggattgcgga
      901 cttggggcgg aaggccttca cgacctcgtc gcgcgtctcg aggtagggtc cgccgatgag
      961 gtcgatgcag tagggcaccg ccgcgaaaat gcccgccacg cgcggccgtc cgtccgcatc
     1021 cttcgcgcct tcgagggtct cgcgaatcga tttcggctgc ccggggaggt tgatgatgag
     1081 cgcgcccctg cggatcaccg cgacctggcg cgacaggatg gcggtgggga cgaactcgag
     1141 gctgatccgc cgcatctgct cgccgaaacc gggcatttcc ctgtccgcca cggcgagcgt
     1201 ggcctccggt gtcacgtcgc gcggcgcggg cccggtgccg ccggtggtga tgacgaggtg
     1261 gcagccggcg cggtcgacga gctcgacgag ggtcgcctcg atcgtcgcgc gctcgtcggg
     1321 gatgaggcgg cgctcgaagc ggacgggatt gaggagcgcg cccgcgagcc aggcctcgag
     1381 cgccgggatg ccctcgtcgc ggtagacgcc ctgggacgcg cgatcgctcg tggccacgag
     1441 gccgatggtg acggggtcgt gcgtgctcat ggcgtgcgct tcgcgtgctc ctcgaggacg
     1501 gcgttgatgg cccggaagag ttcgcgatat cgcttcggcg gccgctccgc aagccgctcc
     1561 tcgagggcgg ctgcggcgag gctgcgcagg cgttgcggat cggccccggg atactcgaga
     1621 gcgaaacgct ccacggcgcc cgggtccgcg aggaggtcgt cccgccactt ttccacgcgg
     1681 tggaagaggg cggtctggtg gagcgagggc gcgtgcaacg cctccagttg cgcggcgatc
     1741 ggggcggcat cgagattgcg catgatgcgg ccgatgagct ggagctggcg gcggatggct
     1801 tcgtgcttcg tcatcttccg gcactcgacg acggcctcgc gcaggtcgtc gggcatttcg
     1861 atgcgcgaga gctggtccgc ccgcagccgg gtgagggtca cgccgatgtc ctgcagttca
     1921 cgggcctgcc tcttgcggcg ggatttgctg ataagattct cgtcgttcat ccggtcatca
     1981 atcgtctgct cgcgcgcctt gggtgcccgc tgaagtttag aggttttggc ggttcggcgc
     2041 gcgaaatctc cgcaggaaag cccccgcgca catgcccgac acgcccgccg tcccggcgct
     2101 tcccctcgat cgtgccgcgc tcgaatccgc cgcgctgcgc gcgctggatc tcgcccgctc
     2161 gcgcggggcg agcgccgcgg aggccgagtc ctccgcatcg gtcgggcagt ccgtgacggt
     2221 gcggctcggc gaggtggaaa cggtcgagta caaccgcgac aaggcgctgg cggtgaccgt
     2281 ctatttcggc gcccggcgcg gcaacgcaag cacctcggac ctgtcgggcg actcgatcgt
     2341 ccgcacggta gatgcggcgt gcgccatcgc acgccatacc gcgccggacg aggccgccgg
     2401 ccttcccgac gcggaccgcc tctatcgagg cgaggccggg gggctcgacc tttatcaccc
     2461 ctggaatctt tcggtggagg atgccatcgt cctcgcccgc gagaccgagg atgcggcgcg
     2521 cgcggtggac gcgcggatca ccaactcgga aggcgccagc gtctcttcat gggacgccga
     2581 cttcgtgttc gcgaattcgc tcggcttcat cggcggcttt ccttcctcgc gggccagcat
     2641 cggctgcgga gtgctcgccg cgcagggcga gtcgatgcag cgtgactact ggtacacgtc
     2701 ggagcgcgac cccgcgcgtc tcgaggcggc gcaggccgtg gggcgccgcg ccggcgagcg
     2761 ggccgtgcgc cgcctgggcg cgcgccgcgt tgcgaccgtc gaggtaccgg ttctcttcga
     2821 ggccaacatc gcgggctcgc tcatcggcca cttcgtgtcg gccgccagcg gctcgagcct
     2881 ctatcgccgc tcgtcgttcc tcatcgatgc cgcgggcaag ccggtgttct cgccgatcgt
     2941 gaacctcgac gaggagccgc acatcccggg cggcatggcc agcggctggt tcgatggcga
     3001 gggcgtggcc acgagcgcgc ggcgggtcgt cgacgcgggc accctcaccg gctatttcct
     3061 gtcgacctac tccgcgcgca agctgggcct gcagacgaca ggcaacgccg gcggctgcca
     3121 caacctcctg ctgcagccgg gccgcgagga tttcgcgggc ctggtgcgcg cgatgggccg
     3181 cggcctggtc gtcaccgaac tcatggggca gggcgtgaat cccgtgaccg gggactactc
     3241 gcgcggtgcc gtcggcttct gggtcgaggg gggcgagatc tgctacccgg tcgaggaagt
     3301 gaccatcgcg gggaacctgc cggagatgta ccgcggcatc attgcggtcg gcagcgacgt
     3361 gctcgtgcgt ggcgccaggt cctcgggatc catcctcgtg gaacgcatga cgctggccgg
     3421 ccactgaccg tcggcgcctc ttctggtacg ctacgcgcga gcggcggagg ccgtcgctcg
     3481 cgccgacaac gccggcagcc aacaggattt cacgaggagt gatgaccatg cagcgcagaa
     3541 agttccttac gtccgcggca acggggcttg cggccggtgc agcgatgatg ccaacccttt
     3601 cgcgtgcgca ggcggccgcg ctgccggagg tcaagtggcg cctcgcgtcg agctttccga
     3661 agagcctgga cacgcttttc ggcaccgccg agttcatgtc caagcgggtc gccgcgtgca
     3721 cgggcggcaa gttccagatc caggtattcg cggccggcga gctcgtcccg ggcctccagg
     3781 tgctcgatgc cgtgcagaac gggaccgtcc aggcctgcca cacggccccc tactacttct
     3841 ggggaaagga tcccgccttc gcgctggatt gcgcccttcc cttcggcctc aacgcgcggc
     3901 agatgaacgc gtggcagata ttcggcggcg gcctggaact cctgcgcgag ttctacaagg
     3961 gctacaacgt gcacaacata ccctgcggca acacaggcgc gcagatgggg ggctggttcc
     4021 gcaaggagat caagaccgtc gccgacctga agggcctgaa attccgcatt tccggttacg
     4081 cgggcaagat catcgagaag cagggcggcg tgccgcagca gatcgccggc ggtgacatct
     4141 acccggcgct cgagaagggc acgatcgacg cggccgaatg ggtgggcccg tacgacgacg
     4201 agaagctggg tttcaacaag gtcgccaagt actactacta tcccggctgg tgggaagggg
     4261 gaccgcaact cagcgcgtac ttcaacgtca aggaatggga caagcttccg aaggagtacc
     4321 agaacatcgt cgaggcggcc tgcggggagg ccaacagctg gatgctcgcc aagtacgacg
     4381 cgcagaatcc ggccgcgctc aaacgcctgg tggccagcgg cacccagctt cgcgcgttct
     4441 cgcgcgagat catggtcgct tcctacaagg ccacgctcga ggtgaacaag gaatcgagcg
     4501 agaagagccc ggccttcaag aagatctacg agtcgatggt gaagttccgc gacgaggaac
     4561 tgctctggtt ccgcgtggcg gacaagggat acgacgactt catgcacggc atcgggtcga
     4621 tcaagtaggg ggcgagcggc ggatcatttc ccgccgcgca gggccttctc gatctcctcg
     4681 gcagccttgt cctccggcgt cttctccttc gaaggcgttg ggggggacgc ctgccccttg
     4741 agctcgcgct cgatcgcttt cgcgggatcc tctttctccg gcgccgtggc cggcggccct
     4801 ttcccgccga ggtccctgag gatgtcctcg gggacatttc cctggtccat ctccgggatc
     4861 tcgaacttca ccttggccgg gtcgaccgtg gactggtcgc tcttgtagac catcaccatc
     4921 tgcgggaaca cgatcacgag cgcgaccatg accagctgga tcaggacgaa gggaatggcg
     4981 ccccagtaga tctggccggt ggtgaccggc gcggtgagct tgcccgtcac cctgtccagg
     5041 tattccttgg ccggggcgac gctgcgcagg tagaaaaggg cgaagccgaa cggcgggtgc
     5101 atgaaggaag tctgcatgtt cacgcccagc agcaccccga accagatgag gtcgattccg
     5161 agcttctccg ccaccgggcc cagcagcggc accacgatga acgacagctc gaagaagtcg
     5221 aggaagaagg cgagcacgaa gatgagcagg ttcaccacga tcaggaaacc gagctggccg
     5281 ccgggcaggc ccacgagcag ctcctcgacc cagacgtggc cgttcacccc gtagaacgtc
     5341 aggctgaaca cgcgggcgcc gatcaggatg aagagcacga acgtcgagag cttggcggtc
     5401 gcgtccatcg cctggcgcag gagcttgaat ggcaggcgcc ggcgggcgat cgccatcacg
     5461 agcgcacccg ttgcgcccat cgcgccgccc tcggtgggtg tcgcccagcc gaggaagatc
     5521 gtgcccagca cgaggaagat gagggccagc ggcggaatga gcacgaacac gacctgttcg
     5581 gccagtcgcg aaagcaggcc caatcgaagc aggcggttgg cgatcgcggc cgcgaaggcg
     5641 aaacccgccc ccgccgcggc ggcaaggacg aggcgttcgt cgagcatcgc ctcgacatag
     5701 gttgccttgt aatagaagcg atcgaaggcc acggccacga gggtcgagac ggtggcgagc
     5761 accacgaagg agggcgtgcc gctggaaccg tccggctcct taaggcgcct cgcgtcgggc
     5821 ggcagcgccg gggcccacgc gggtttcacg aacgtgaccc cgatggcgta gagggcatag
     5881 aggccggtga gggcaatgcc gggaatgaac gcgcccgcgt acatgtcgcc caccgacttg
     5941 ccgagctggt cggccatgat gatgagcacc agcgacggcg ggatgatctg cgccagcgtg
     6001 ccggacgcca cgatcacgcc cgaggcgaag cgccggtcgt agccgtagcg aagcatgatc
     6061 ggcagcgaga tgagccccat cgagatcacc gaggccgcca caacgcccgt ggtcgccgcc
     6121 agcagggctc ccacgaagat caccgcgaag gcgagcccgc cgcgcagcgg cccgaagagc
     6181 tgccccaccg tgtccagcag gtcctccgcc atcccgcttc gttcgaggat cagtcccatg
     6241 aacgtgaaga agggcacggc gagcagggtg tcgttggaca tgacgcccca tacgcgctcg
     6301 ggcagcgcct ggaagagcgc cggctgcagc aaccccagct cgatgcccac gaggccgaaa
     6361 atgatgccgt tggccgcaag cgcgaaggcg accggatatc cgaggaggag gaataccacc
     6421 agcgccgcga acatgatcgg cgccatgttg tggacgagga actccatcag gacctgccct
     6481 cggcgagctt gcggatatcc tcggcgagtt ccagctcggc cgagcgccgg tgcaggatat
     6541 cgatcgggtc cggcgccacc ccgcgcagga agccgatccg cttgacgagt tccgagaccg
     6601 cctgcagcgc gaggagcagg aagccgatgg gcacgatcag ccgcgccggc cagatggcca
     6661 gcccgccggc gctccccgac atctcgtcat ggacccaggt gcgcacgaac accggccacg
     6721 agagatagat gaagatcagg gccatcggca acaggaagaa cagggtgccc agtacgtcga
     6781 tccacgcctg cgtccggcgc gacagccgcc cggcgatcac gtcgatgcgg acgtgctcgt
     6841 tgcgcagcag cgtgtagccg gcgcagagca tgaacaccgc cgagaagagg taccactgcg
     6901 cctcgaggaa ggcgttcgag ctgtagttga agaccttgcg gatgatcgcg ttgcccgcgc
     6961 tgatgagcac cgcggcaagg atgagccagt acacggtccg gcccacgcgc tcgttcagcg
     7021 cgtcgattcc ccgggaaagc ttcagcagtg cttgcatctt ccctcctaga atgccggacg
     7081 atcgtcgcgc gcggtgtcct ccggcacggc catcgcgccg ccgagccgcg gtgggggcga
     7141 ttatagggga cagtccgccg ccgccgggtt tcccgcgggc cgcatgctag aatcccccac
     7201 gctttcaatc acttcccgag gtcccggtcg acgccgggcc ggcccatgtt ccccgacacg
     7261 cacaccatag ccgccgtcga tcccgagatc tggcaggcca ttcgagacga gtaccgacgc
     7321 caggaagacg gcatcgaact catcgcttcc gagaactacg cgagccccgc cgtgatggcc
     7381 gcgcagggca ccatcctcac gaacaagtac gcggagggtt atcccggcaa gcgctactac
     7441 ggcggctgcg agttcgtcga cgtggcggag agcctcgcca tcgagcgcgt gaagaagctc
     7501 ttcggcgccg agtatgccaa cgtgcagccg cactcgggct cgcaggcgaa ccaggccgtg
     7561 tacatggcca tgctcaggcc gggggacacg atcctcggca tgtccctcgc gcacggcggg
     7621 cacctcacgc acggcgcggc ggtgaacctt tccggcaagc tctaccattc cgtggcctac
     7681 ggcctgagcg aggccgagga gatcgactac gaccaggtgc gcgacctggc gcgcgagttc
     7741 aacccgcgca tgatcgtggc gggggcctcg gcgtacgcgc tgcgcgccga ctggcagcat
     7801 ttccgcgaca tcgccgacga ggtcggcgcc atgctgatgg tcgacatggc gcactacgcc
     7861 ggcctcgtgg cggcgggcct ctatcccagt ccggtcggca tcgccgattt cgtgacgagc
     7921 acgacgcaca agacgctgcg cgggccgcgc ggcggcatca tcctcgcggc cgccgagcac
     7981 gagacggcgc tcaactccgc gatctttccc accatccagg gcgggcccct gatgcacgtc
     8041 atcgcggcca aggcggtcgc cttcaaggag gctctcgagc ccgcgttcaa gtcctaccag
     8101 gcgcaggtcc tcgcgaatgc ccagcgcatg gccaccaccc tcgcgcaacg cggcctgcgc
     8161 atcgtctccg ggcgaaccga gagccatctc ttcctcgtcg acctgcgcgc caagaaggtc
     8221 accggcaagg acgccgaggc cgcgctcggc aaggcgcaca tcacggtcaa caagaacgcc
     8281 attcccaacg accccgagaa gcccttcgtc acgagcggca tccgtgtcgg ttcgcccgcc
     8341 atgaccacgc gcggcttcgc ggacgccgag gccgagcagg tggcccacct catcgcggac
     8401 gccctcgagt cgcatgcgga cgatgccggg ctggccggcg tgcgcgcgaa cgtgaacgcg
     8461 ctttgcgcga agtttcccgt ctatggcccg gccatgcgcg ccacctacgg gctggcctga
     8521 tccgggcccg ggcgacccgc gatgaaatgc cccttctgca gcgccgagga cacccaggtg
     8581 atcgactcgc gcgtgagcga ggagggcgat tccatccggc gccggcgccg ctgccaggtg
     8641 tgcgccaagc gcttcacgac ctacgagacg gcggagctgc gcatgccgca gatcgtgaag
     8701 ggaaacggcc agcgcgagga cttttccacg gccaagctgc gcacgggctt ccagcgtgcg
     8761 ctgcacaagc ggccggtcac caccgagctc gtggacgcgg ccatcacgcg catcgagcag
     8821 aagctgctgg cgctgggcga gcgcgaggtc gattcgcgcc gcattggcga aatggtgatg
     8881 gaagaactgc gcaagctcga caaggtggcc tacatccgtt tcgcatccgt ctatcgcagt
     8941 ttctcggacg tcgatgactt ccgcgacgcg atccgcgagg tgcgggacaa gtagcgtgag
     9001 cttccccgcc gaggaccatg ccctgatggc gcgcgccctg gcgctcgccg cgctcgggcg
     9061 cgacaccgcc acgccgaatc cgaacgtggg ttgcgtgatc gcgaaggccg gccgtgtgat
     9121 cggcgagggc tggcacgcgc gcgcgggcga gccgcacgcc gaggcgatgg ctctcgcggc
     9181 ctgcacggaa tcgcccgagg gcgcgactgc ttacgtgacg ctcgagccgt gcagccacca
     9241 cggccgcacg ccgccttgcg ccgatgcgct cctggccgca agagtggcgc gcgtcgttgc
     9301 cgccctcgag gatcccaacc cgctggtgac ggggcagggc ttcgcgaagc ttcgcgccgg
     9361 cggcattcgc gtggagtcgg gactcctcgc cgccgaggcg aaggaggttc acctggggtt
     9421 cctcgcgcgc atgtcgcgcg gcaggccgtg gatgcgcatc aagtccgccg cctccctgga
     9481 tggccggatc gcgctggcca acggcgaaag ccgctggatc accggcgagg cggcgcggcg
     9541 ggacgtgcac gcgttgcgcg cccggtcctg cgccatgctc acgggaatcg gcaccgtgct
     9601 tcgcgacgac ccggagctca ccgtgcgtca cgtcccctgc tcgcgccagc cgcgtcgcgt
     9661 cgtcatcgac agccggctcg agctgccggc cgccgcgagg atcctctcgg gcgagccgcc
     9721 cctcgtgctg accgtgagcg aggatcgcgg gaggcgtgcg gcgctcgagg cccttggcgc
     9781 cgaggtcgtc gtcgtgccgg ccgaaggggc caagaccgac ctcggcgccg tcgcgcgctt
     9841 gcttgccgat cggggattca acgaggtcac cgtcgaaacg ggggccaagc tgaacggctc
     9901 gctgctgcgc gcgggcatcg ttgacgagat cgtgctctac ctcgcgccac tgctcctggg
     9961 cgatgctgcg cagggccttt tcgcgttgcc ggtgttcggg caactcggcg aggcgctgcg
    10021 cccgcgcatc gtcgacatcc gcgccgtcgg cgcggacctt cgcatcaccg cccgactgga
    10081 gacatgacat gttcaccggc atcgtgaccg ccatcggccg catcgcctcg gcacgcaggc
    10141 acggggacgg cctgcgcctg cgcatcgatg cgccagggtt cggcatggac gacgtggcgc
    10201 tgggcgactc gatcgccgtg cagggtgtct gccataccgt ggtggcgaag gacgccagcg
    10261 ggttcgacgt ggacacgtcg gctgcgaccc tcgccgtgac gacggggctg gcggaaggtc
    10321 gcgaggtgaa cctggagaaa tcgctcacgc tggccgaccg gctcggaggc cacctcgtga
    10381 gcggccacgt cgacggcgtg ggcaccgtca ccgccttcga cgatctcggg ggaagctggc
    10441 gcctcgagat ccggatgccg caggagcttt cgcgttatgt cgcgcgcaag ggttcgatcg
    10501 ccgttgacgg cgtgagcctc acggtgaact cggtaaccag cgaccggttc gaggtgaaca
    10561 tcatcccgca cacgcgatcg gccacgacgc tgcgccgcct ggctgccggc agccccgtga
    10621 acctcgaggt cgacatgacc gcgaggtacc tggagcgcct tctcggggcg cgggagcagg
    10681 gcattcctcc gggatcgctc cctgaatctt gacctagggc aaaccctgtc gagaaagcgg
    10741 caggcgctcc gcgatggccc ctggtgcagg aggagaatat ccgtgttgca gtccggaagg
    10801 agttgcgcat ggtccgaagc gagttggtgc cgatgccggg gaaggccggg caccgggcat
    10861 gcatcggatt cctggcagcc atcgcctgcc tcgcgctgac agtggcctcg gcgcgccagt
    10921 tgcccgttcc ctggcctgtc ccgccctttg cgtcgtcacc gcacaggctt gccgccgctc
    10981 ccgcgtggga ttgctggatc gaccccggtg aaggaggcgg catccgctgc atcgcggacc
    11041 gcgacgtccc gcttcccctg cccgacgaat tcaccgacga cgacgaggcg gcagaggtcc
    11101 tgctcgaact tctccacgac tacctgcacc gcgcgatgcc gcagcgggca tcccagctgg
    11161 ttcgcgaaag gtactacctc ctgcgccgcg acgacttgtg gacgatccgc ctgagcagcc
    11221 cgcctttcga gacttcctgg agcgacgaga ggccgcagct gctggtgcgc tcgctgttgt
    11281 gccgtggcgc gccggggtgc cccatccggt tccaccactg agggcgcgca cgctgttcgc
    11341 gggcggttgc ccgcccgatc gggcaaaata gagggtctgc atggagagac ccccgtgaac
    11401 ataagcccga ccgcagagat catcgacgac atccgccatg ggcggatggt cgtgctcgtc
    11461 gatgccgagg accgcgagaa cgaaggcgac ctcgtgctcg ccgccgattt cgtgactccc
    11521 gagacgatca atttcatggc cacgcatgcg cgcggcctcg tctgcctcac cctcaccgag
    11581 gagcgctgcc ggcaactggc gctcccgctg atggtgagcg acaaccgctc gccgcatggc
    11641 accgcgttca ccgtctccat cgaggcggcc gtcggcgtga gcacgggcat ttccgcggcc
    11701 gaccgcgccc gaaccgtgcg catggcggtc gccaagcatg cgaagcccgc cgatatcgtc
    11761 cagccggggc acatctttcc ggtgatggcg cagaacggcg gggtgctggt gcgcgcgggg
    11821 cacacggagg ccggctgcga cctcgcggaa ctggcggggc tcacgccggc cgcggtcatc
    11881 tgcgaggtca tgaagccgga cggggaaatg gcgcggctcc cggacctcgt cgagttcgcc
    11941 gcgcggcacg gcctcaagct gggcaccatc gccgatctca tccgctaccg cagcgagaac
    12001 gaaaggctca tcgaccgggt ggccgacaag acgatcgcca cgccctacgg cccgttccgc
    12061 ctgtgcgtgt accacgacag ttccgcgggc gagctgcact atgcgctggt gaagggcgaa
    12121 ccctcgccgt cgcggccggt cctggtgcgc gttcacgagc ccttccactc gatcgacatg
    12181 ttcgacttcg actcgagccg gcacgcctat tccgtgcagg attcgatgcg catcatttcc
    12241 caccacaagg agggcgtgat cgtgctgctg cgccgccacg aggcgcccga ggaggtgctg
    12301 gagcgattct cgatgcacgc cgcgcccgcg cgcggcgtgc gcaagtggga cccgaaactg
    12361 catggcatcg gggcccagat cctgcgcgat ctcggcgtcg ggcgcatgcg cgttctcgcg
    12421 cggcccaagc gcatcccgag catggcgggc ttcggcctgg aggtggtcga gtacgtgtcg
    12481 ccggacgacg catcgcaggt aaaggcgata tgaccgccta cacttcggaa ttgaaggggg
    12541 aagggctcac cgtcgcgatc gtctggagcc gcttcaacga ggatatcgtc cgcgagctcc
    12601 tgaccgcctg cgaccgcgag ctcgtcaacc tgggcgtggc gacgggcgac atcgacgtgg
    12661 tgagcgttcc cggcgcactc gagatcccgc tggtgctgca ggcgctcgcc ctgcgcacgc
    12721 gcggattcac gcgtcgctat gacgcgctgg tggcgctggg tgcggtgatc cgcggcgaga
    12781 cctaccattt cgaggtcgtc tcgaacgaaa gcgcgcgcgg catcctggac gtgcaactgg
    12841 agaccggcgt accggtcgcc aacgcggtcc tcaccacgag cacggaagag caggcgcgcg
    12901 agcgtgcggc ggagaagggc gcggaggccg cgcgagtcgc ggtcgagatg gccaacctcc
    12961 tcaaggcggt gagcggatga gaaccccgcg acgccgggcg cgcgagttcg cgcttcaggg
    13021 cctttaccaa tggctgctca cgggcgcgcc tgccggggaa atcgcgcgca acctcgcgga
    13081 actcgagggc tatggcgcgg ccgacgaggc gttcctcaag gcggaactcg ccggcgtgat
    13141 cggcgaggcc gagggcctgc aggcgttgct cgcgcccctg gccgaccgca agtgggcgga
    13201 agtgagcccg gtggaaaagt cgatcctgct catcggcgcg tgggaaatcg cgcacgtgcc
    13261 ggagatgccc taccgcgtgg cgatcaacga ggccatcgaa ctcgcgaagc gcttcggcgg
    13321 catcgacggc cacaagtacg tgaacggcat cctcgaccgc gtggccgccg ccgtccggcc
    13381 ggaggaaatc gccgccgggc gcgcggccga caggaagccg cggcaggctt ccagggctcc
    13441 cgccggggcc accccaccga aaaaggcaga agcatgatcg agaaatcccc cgagttcgac
    13501 ctgattgccc gccacttcac ccggccggcg cgcaatgccg tcctcggcgt cggcgacgac
    13561 tgtgcgctcg tcgacgtttc caacgggatg gaccttgccg tatcgaccga cacgatggtg
    13621 tcgggtacgc acttctttcc cgacgtcgac ccggaaacgc tcggtcacaa ggcgctcgcg
    13681 gtgaaccttt ccgacatggc ggcgatgggc gcgatgccct actgggccat gctcgccctc
    13741 acggttcccg acgtgaacca cgagtggctt gccaagttcg cgaagggctt cttcgacctc
    13801 gcggaggaat tcggcgtctc gctcatcggt ggcgatacca cgaaggggcc gctcaccatg
    13861 accgtcacca tcatgggcga agtgccggcc ggtgcggcat tgcggcgcgg cggcgccaag
    13921 gccggccatg acatctgggt gtccggcaac ctgggcgacg cggctctcgc ggtggcgcac
    13981 cggcgcgggc tgctcaagct ctccgagggc gagtacatgg aagcggtgat gcgcctgtac
    14041 gagccggcgc cgcgggtcaa gctcggccag gcgctgcgcg gccttgccac gagcgccatc
    14101 gacatctcgg acggcctgct tgcggacctc gaccatatct gcactctctc gaaggtgggg
    14161 gccaccgtcg aggtcgagcg cctgccgctt tccgcaatcg gtgccaagca tatcgacagc
    14221 cccgcgggcc tgaccgcgat cgtggccggc ggcgacgact acgagctgtg cttcacggcg
    14281 cctgccaccg cgcgcgacag catcgaggac cttacggaag tcctgggcgt cccgctgacg
    14341 cgcatcgggg agatccgcaa gggcaagggc gtatcgctgg ttggtgcgga tggaaagccg
    14401 atcgcggtcg acgggcgtgg attcgaccac ttcacggcgt cctgacgcgc gattcctcgt
    14461 cgcgcacccc gcgcacttca tcgccctggg cttcggggcg ggtctgtcac cgcgcgctcc
    14521 gggtaccgtc ggcacgctgg ttgcgctggc gctctactgg attctcgcga tcgcgctgcc
    14581 cccgctcgcc atcgccttcc tggccgtgcc gctcttcttc gtgggggtgt gggcctgcgg
    14641 cgtggcggga aggaacctcg gcgtagaaga ccatggcgcc ttcgtgtggg acgagattgt
    14701 cgccttcctg ccccttgctg ccctggcctc ggcaagcccg tggctgcagg cgctcgcgtt
    14761 tgcgctcttc cgcctcttcg acatctggaa gcccttcccg atccgccagg tcgaatcgcg
    14821 cgtgaaggga gggctgggag tcatgctcga cgacctgctg gcctcctgct acgcctacct
    14881 cgtcctggtc atcctcgtgg tcgcggtcca tcgcttcccg ccggcgccat gaggaagacc
    14941 tggccctatc cccaactcgt tgcccatcgc ggcggtggcc ttctcgcccc ggaaaacacg
    15001 ctcgccgcga tccgcctcgg ccagtctctc ggtttccggg cggcggagtt cgacgtgaag
    15061 ctcacgcgcg acgacgtggc cttcctgctg cacgactcca ctctcgatcg aacgacgtcc
    15121 ggctccggcg cggccgggga tcgcaactgg gaggagcttg cggcgctgga cgctggcggt
    15181 tggcattcgg aaacgttcag cggggagccc atcgcgcgct tcgacgaagc ggcgcggctg
    15241 cttcgctccg cgggcacgat cgcgatcgtg gagatcaagc ccgtgcccgg aacggaacga
    15301 gcgacgggac ttgcggtggc cgcccaggcg caggcccact ggcgggatgc ggaagtgccg
    15361 cccctgctgt cctcgttttc gcgcgagtca ctggcggcgg cgcgggagtc cgcgcctttg
    15421 cttccgcgcg gcctgatcgt cgaagcgccg accgacgccg atttcgcaag cctcgactcc
    15481 ctcggcgccg tgtcgctgca ctgcgggcgc aagtacgcca cgccggaatt gttcgaccgc
    15541 gcccatgcgg ccgggtaccg ggtcctggtc tggaccgtga acgatccccc cgaggcgaaa
    15601 cggctgctcg cgtggggcgc cgatggaatc atcaccgaca atctccgcca gtttgcgacc
    15661 cggtttcccg atcgcctcta gcctgctttc cggttcatca agcccttgca gcgggttctt
    15721 caggcagggc ggcaagattt gttgccaagc cactgatttc gagcctaata tgaatccaaa
    15781 aaccccgcct tcccccggcg accccgggtg acgcctctac gcacaggaca ttccggcagg
    15841 ttcccgtggc ccgcatcgct gtcttcaacc agaagggtgg cgtcggcaag acgacgacgg
    15901 cgctgaacct ggccgccgcg atcgctgccc ggggcggacg gccgctcgcg atcgacctcg
    15961 atccgcaggc gcacttttcg gccattgccg gcgcgttgcc gcgcacggcg gagggcagca
    16021 tctatgccct gtaccgcgac gcgcggccgc tgcgcgaact cttcgtcgat tccgcgctag
    16081 gctgggtggt catcccggcg catctcgagc tggccaaggt ggatacccag ttcggcaagg
    16141 gccccaacat cctcaatcga ttgcgtgccg ggcttgcgcg cgaggggttg ggaaggacgg
    16201 gcagcgtgct catcgattgc agcccgatgc tgggcgtgct gtcgctgtcg gccatcttcg
    16261 ccgcggaccg ggtgctggtg cccgtctcga cggactacct cgcggtgaac ggcgcgatgc
    16321 aggtcgaccg cacgctgaac gccctgcaac gcgtggtcgg gcggcgcatc gagcgccgct
    16381 acgtgatcac ccgattcgac gggcggcgca ggatgtcgtg ggacattctc gcgaccctca
    16441 aggcgcgatt cggtgcggat cttgccgaaa gccgcatctc ggaaaacgtg agcatcgcgg
    16501 agagccccta caaccggcgc gacgtctttg cccatgcgcc cgcaagccgg ggtgcagcgg
    16561 actaccgcgc catgttcgag gagctggaat cgtcgggctt cctggatgag tttccggtgg
    16621 ccgggggcat gctcgccgcc gtcgcataga ccgcaatcag ccgccgtcgc gcgatccctc
    16681 gtcggcgatc aggttgatga ctccttcgaa gtcggatccg tccacgggcg cggattcccg
    16741 caactcgcgc gcctccagga ttacgcaatg gtgatagacc tgccggagct tgcgctcggc
    16801 ctcgtcgcgg tcgcgaccct ggatcgtcag gttgtcgacg accgagcccc cgcgcgtgcg
    16861 gatgcggaag ccgaacttct tcatttcgac cagaaccacg gcacctccgg gatggcccgc
    16921 ggggggccgc ttacgcggcg atggcctggg gcgatcgcag cttcgcggcg aggtcccgga
    16981 gcgcggtccg caggccctct tcgaccaccg ggtgatagaa gggcatctcg agcatcgaag
    17041 cgactgtcat tctgttcgcc agggcccagg caagcaagtg gccgatgtgc tcggccgccg
    17101 ggccggccat ctcggccccc aggaaccgcc cggtagcgcg atccgcgtag acgcgcaggt
    17161 ggccccggtt catgcgaatc acgcgcgcgc ggccctggtt ctcgaacgag acctcaccga
    17221 cctcgaagga accgggcacg agcttcgcgt acccgccgcc cacgatcgcg atctgcgggt
    17281 cggtgaagac caccgcgagg ggcgcacggc gcaggccggg ctgcaccgcc ggaaaccccg
    17341 cggcgttatc gcccgcgatg cggccctcgt cggcggcttc atggaggacc gggatgtagt
    17401 tcgaggcatc gccggcgatg aagaggttcg tgtcgcccac gcgcagggta tgcgtgtcga
    17461 agcgcgggac acctgcctta tcgagctcca gccccaggga ctccagcccc agcccgtcga
    17521 cgttcggccg gcggccggtg gcggccagca cgtagtcgaa agtctcgacg caagccgcgc
    17581 tttcgaggtt cgtgaattcg acctcgacgc cctgtgccac gcgggtcacg cgcttcacgt
    17641 gcgcatccgg atcgaggtag agctccgagg cgatggcgcg cagggcggca tcccgcacca
    17701 cggggtcggt gagggggccg acgcgcccgc cgcggcccag cacgaccacg cgcacgccga
    17761 ggcgggcgag ggcctggccc agttcgagtc cgatcacgcc ggggccgaag acggccacgg
    17821 agcggggcag ggtgtcccac gaaaacacgt cgtcgttcac cacgaggcgg tcgccggcgc
    17881 cctcgagcag ggccggaacg gtgggccgcg aaccggttgc gatgacgatc gcgcgcgccg
    17941 tgacacgcgt gtgtccgtcg acgtcgagcg tgttgcggtc cacgaagcgg gctcggccga
    18001 ccacgcggtc ggcaggggga atttcctcga cgccattgag cacgaagccg acgaagcggt
    18061 cacgttcgcg cttcacgcgg tccatcacct ctcggccatc gacccgcttg gttccggtga
    18121 cgtgcacgcc gaatccgccg gcgtggtcga tcgcgtggga ggcttccgcc gccgcgatga
    18181 ggagcttgga gggcatgcag cccacgcgcg cgcaggtggt cccgtacggt ccgccctcga
    18241 tgagcacggc acgggcgccg cgcgcccgcg cggcac
//
LOCUS       DASUEV010000041        11137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1043147, whole genome shotgun sequence.
ACCESSION   DASUEV010000041 DASUEV010000000
VERSION     DASUEV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043147"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..906
                     /gene="queE"
                     /locus_tag="VFV55_01365"
     CDS             253..906
                     /gene="queE"
                     /locus_tag="VFV55_01365"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX4942967.1"
                     /translation="MRIMATSMYAAKEIFHTLQGEGANAGSPAVFCRFAGCNLWSGRE
                     ADRAAAICNFCDTDFVGTDGPGGGRFGSAEALAGAVEAAWPAKGARERLVVCTGGEPL
                     LQLDAPLVRALHAKGFRIAIETNGTLPVPEGIDWICVSPKSTAPLVQATGDELKLVYP
                     QADAPPELFEALAFRHFFLQPMDGPAREANTRAAVAYCKAHPRWRLSLQTHKLIGIP"
     gene            910..1272
                     /locus_tag="VFV55_01370"
     CDS             910..1272
                     /locus_tag="VFV55_01370"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX4942968.1"
                     /translation="MQISYRFGFDAAHHFPGYPAGHRYHGVHGHSFQAEVAIRGTPDP
                     ATGFVADLEAVERECLEARAELDHRLLNDIEGLDRPSLENLCLWLWTRLAPRLPALAL
                     VTVRRDSCGQACTYTGPA"
     gene            1296..2261
                     /locus_tag="VFV55_01375"
     CDS             1296..2261
                     /locus_tag="VFV55_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4942969.1"
                     /translation="MKTLAVALAAAVLAAPSIALPQSWPTKPVKIVVPFAAGGPADIY
                     ARAVGEKLSQVLGQSFVIEDKPGGGSIVGTDLVAKSAPDGYTLLMMSNTHTVNESLIP
                     DKPFVLMRDFVPVAPVNYSDLVMVVHPSVPAATVKEFVSLAKAQPGKLNYASSGPGTP
                     YHMAGELFKAMAGVDVVHVPYKGSSGARTDILGGQVQMMFDAITTMAPQVRAGKLRAL
                     GTTGKVRSSVLPEVPTVSEAGVAGYETVIWLGILAPAGTPRAIVERLNSEITKITAMP
                     EMREAWAKQGATAMSMTTEEFGRYLREDIEKWSRIVKISGAKADR"
     gene            2263..3399
                     /locus_tag="VFV55_01380"
     CDS             2263..3399
                     /locus_tag="VFV55_01380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4942970.1"
                     /translation="MKLDILSAGAAQGLAGAVAKQAGVELAGTFGAVGGILERYLAGE
                     ACDVVILTHAQVAQLTARNRVALDFCSDLGVVRTSIAVRKGDAKPDVSTPSALRAALL
                     SADAIFLPDPHKATAGIHFARVLATLGIAGEVAARLRPHANGMTAMRALSEAGGHPIG
                     CTQTTEIVATPGVALVAPLPKEHELATVYTVALKSGPASAAARDFAQLLTGPATAAAR
                     AAAGFEGAAIRRATAADATAARELVFGVLAEYGLTPDPNDTDADLMDLDAHYLSRGGS
                     FDVAIAPDGRLVACCGLRPMAGGAVELRKMYARREARGQGIGRRLLDRALAVARASGS
                     PRVELETASVLKEAIALYRKAGFVPRPGKPDTRRCDLAFTLDLS"
     gene            3402..4601
                     /locus_tag="VFV55_01385"
     CDS             3402..4601
                     /locus_tag="VFV55_01385"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX4942971.1"
                     /translation="MPILANLTKCRVPVKAWTDDLEPQARRQLENVADLPIVFGHVAA
                     MPDVHAGIGATVGSVIPTKAAIIPAAVGVDIGCGMNAVRLSLAAADLPQNLGRVRSAI
                     EAAVPVGFDQHGECPVEDAGSLRAMSRRLDAIARKHPGLTKLQRSFDTTWARQLGTLG
                     GGNHFIELCLDESMRVWVMLHSGSRGVGNAMGRYFIERARREAERLDRRLPDRDLAWL
                     DEGTPLFADYVEAVGWAQDYALANRREMMLLIVEALRRHLPAFSIANEAINCHHNYVA
                     RERHLGEDLFITRKGAIRAGLGELGIIPGSMGARSFIVRGLGNEQSFCSCSHGAGRRM
                     SRTEAKRRFSRQDLERQTEGVECRKDGGVIDEIPGAYKDVGLVMAQQSDLVEVVHTLK
                     QVICVKG"
     gene            complement(4611..5051)
                     /locus_tag="VFV55_01390"
     CDS             complement(4611..5051)
                     /locus_tag="VFV55_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942972.1"
                     /translation="MELRVSALAFCCLVSTLASAGPVVRWHCAYASTEVEAVLCQRVE
                     APDGNVTAPSLPGDSSPMPRLAADILDALDTLYGETIAIPLYSRPVDMARVGILARDV
                     MCGKRPDCEVEFSASRNGLADSSATAKPPAEGMPPPMMTVRRDR"
     gene            complement(5267..6508)
                     /locus_tag="VFV55_01395"
     CDS             complement(5267..6508)
                     /locus_tag="VFV55_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX4942973.1"
                     /translation="MGIVDRVKNIILTPKSEWPIIAGETSSTGDLMGGYVAPLAGISV
                     LCGFVGSSIVGVSLPFIGTYRSPILAGIGVAVFSFVMAFVGIFILSLIINALAPTFGG
                     EKNPAQALKVAVYSYTPAWIAGILGIIPALGLIGVLVSLYGLYLLYLGLPRLMKNPEE
                     KSIGYTAVVVICAIVIGFVISLMAGGLAALTGGGAMMGAMHGRSPGMTFDKDSPMGKL
                     EGFGKQMEEAGRKMEAAQKSGDPNAQMKAAMEGLGTVLGGGKRFEPLGIEQLKPLVPE
                     RLADLPRTSQSAEKSGMAGLQVSKAEAQYDDGAGRHVDLEVTDTGGAGGLMGLAGWMN
                     IQGEKETDDRIERTRRDGKRMVHEEISKRGGDSEYTVVVGDRFVVSARGQGVPIETLR
                     SAVGSIDLAKLESMKDVGAVK"
     gene            6568..8187
                     /locus_tag="VFV55_01400"
     CDS             6568..8187
                     /locus_tag="VFV55_01400"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX4942974.1"
                     /translation="MASRAASVPLFVLALGLAACSGGGGGGSAPVASLPPLQPVTSTV
                     SGVSPFAAGCGDANGTLFVNAEVEPSLAADPANPGHLLAMWQQDRWSNGSARGLVSAV
                     SFDGGATWSRGAMAFSRCGGGTAANGGDFDRATDPWVDFGPGGTAWAMSLSTTGASSA
                     PGSTNAMLVARSADGGRTWGNSVPLIADNELFFNDKNALTADPNDARFAYAVWDRLGQ
                     SGGGPAMFARTVDGGNSWEPARILFDPGTAAQTIGNLVVVVTGGALVNVTTRIEEPAG
                     RPRTASIIALRSVDRGTTWSDPVQVATHLAVGARDPETGAAIRDGAILLNAAAGPGGT
                     LHVVWQDSRFTRGLRDAIAYSRSDDGGLTWSAPVRVSPDATVSAFTPTVRVLADATIG
                     VTYFDLRDNTASPATLPAGYWLARSADGVTWTETRLAGPFDLATAPNAGGLFLGDYMG
                     LAGSGSAFLSLFVRTTGDLANRNDVFFVRAAATSAAIAQGKGIASDGAWPGRWVAKAA
                     APFEVDAAWGARVDAAIARALAARGRPSPSR"
     gene            complement(8188..9012)
                     /locus_tag="VFV55_01405"
     CDS             complement(8188..9012)
                     /locus_tag="VFV55_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX4942975.1"
                     /translation="MAETRNLAVFCDFENVALGVREAKYDKFDINKVLERLLVKGSIV
                     VKKAYCDWERYKEFKAQMHQASFELIEIPHVRQSGKNSADIRMVVDALDLCYTKSHVD
                     TFVIVSGDSDFSPLVSKLRENAKTVIGIGVKKSTSDLLIANCDEFIYYDDLVREHPAK
                     RQSAKKVEGKPAAPVKDKEKERENGGGTGKKQEAIELVLETIDDLVDERGVEETLWGS
                     MVKQAIKRRKPGFSESYYGYRSFNQLLEELQERGLVELERDEKSGGYTIRSVAPDE"
     gene            9054..9899
                     /locus_tag="VFV55_01410"
     CDS             9054..9899
                     /locus_tag="VFV55_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4942976.1"
                     /translation="MLSAGSDNLIEIRDLHFAYGKRPVLKGLELDIPKGKVVAILGAS
                     GCGKTTLLQLIGGALAPSRGSVKVHGLEVHTLDHEGLYRLRRQIGMMFQKGGLFSDLT
                     VFENIAFPMREHTSLPDEIISDLVKMKLNAVGLRGAHRLYPTELSGGMARRVALARAI
                     ALDPDVLIYDEPFAGLDPITLNVICNLIRALNDALGSTSVVVTYDVPEAVKLADYLYV
                     MGDGRIVGHGPTQEMLASDDPFVRQFLHAETDGPVPFHFPARPIAEELELAPARRPAR
                     SVRAL"
     gene            9959..10927
                     /locus_tag="VFV55_01415"
     CDS             9959..10927
                     /locus_tag="VFV55_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4942977.1"
                     /translation="MIHRLAGAACAALAFALPALAQTDYPTRTITMIVPFPPGGVADI
                     TARPAAEAMGRYLKQTVIVENKSGAGGGVGMQYVSRAKPDGYTLLLALSSISIIPEAD
                     RILGRDPMYQLSQLVPIARFTADPTVLAVRADSPWKSAREMLEAAKKAPGTIPYGSSG
                     NYGTMHVPMEMLTGAAGAKMLHVPFTGAGPAVVALLGGNVDALSTGPSSIMGHLKGGK
                     LRVLASWGETRHPALPDVPTLKELGYDATFSQWTGLFVPAGTPEAVIAKLREAARATA
                     SDPTFLASLARVETPLQYLDQPQFRNFWDADAKKLSDVVKKIGKVE"
BASE COUNT         1733 a         3905 c         3713 g         1786 t
ORIGIN      
        1 gttgaggccg agcaggccga tcttggcgcc gggaaagaac gagagcgaaa tgtccttgag
       61 gatctgccgc ttgggcggga cgaccttgcc cacgcggttc atcgtgaaaa cgtattgcgc
      121 catggcggta ctttcgggcg aaaaggtgcg aagcgagcgg aaagccttct attttaaccg
      181 accggcgctt cccgagtgct tcctgggcgc tttgcggcca tatcgaggcc gccgggagga
      241 gcggtgaaaa aaatgcggat aatggcgacc agcatgtatg ccgccaagga aatctttcac
      301 acgctgcaag gcgagggcgc caatgccggg tcccccgcgg tgttctgccg tttcgccggg
      361 tgcaacctct ggtccggccg agaggcggac cgcgccgctg ccatctgcaa cttctgcgat
      421 accgatttcg tggggaccga tggtccgggc ggcgggcgtt tcgggtcggc cgaggccctg
      481 gccggcgcgg tcgaggccgc gtggcccgcg aaaggcgcca gggagcgcct cgtcgtgtgc
      541 accggcggcg agccgctcct gcaactggat gcgccgctcg tccgggcgct gcacgcgaag
      601 ggcttccgga tcgcgatcga gaccaacgga accctgccgg tgccggaagg catcgactgg
      661 atctgcgtga gccccaagtc gacggcaccg ctcgtgcagg caaccggcga cgagctgaag
      721 ctggtctatc cccaggccga cgcgccgccc gaattgttcg aggcgctcgc cttccggcac
      781 ttcttcctgc agccgatgga cggcccggcg cgcgaagcca acacccgcgc cgccgtcgcc
      841 tactgcaagg cccatccccg ctggcgtcta tcgctgcaga cgcacaaact gatcggcatt
      901 ccctgaccca tgcagatttc ctaccgattc ggcttcgacg ccgcgcacca cttccccggc
      961 tatccggccg ggcaccgcta ccacggcgtc cacggccact cgttccaggc ggaggtggcg
     1021 atccgcggca cgcccgatcc ggccaccggc ttcgttgcgg acctggaagc ggtcgaacgc
     1081 gagtgcctcg aggcgcgagc cgaactcgac caccggctgc tcaacgacat cgaggggctc
     1141 gacaggccga gcctcgagaa cctctgcctg tggctgtgga cgaggctcgc gccccgcctg
     1201 ccggccctgg cgctcgtgac cgtccgccgc gactcctgcg ggcaggcctg cacctacaca
     1261 ggtccggcgt gaggccggca tacaaggaga agaccatgaa aacgctcgcc gtcgcccttg
     1321 ccgccgccgt tctcgccgcg ccctccatcg cgctcccgca gtcctggccc acgaagcccg
     1381 tgaagatcgt cgtgcccttc gccgcgggcg gcccggccga catctatgca cgcgcggtcg
     1441 gcgaaaagct ctcccaggtg ctgggccagt ccttcgtgat cgaggacaag ccgggcggcg
     1501 gatccatcgt cggcaccgac ctggtggcga agagcgcgcc cgacggctac accctcctca
     1561 tgatgtccaa cacgcacacg gtcaacgagt cgctcatccc ggacaagccc ttcgtgctga
     1621 tgcgcgactt cgttcccgtg gccccggtca attactcgga cctggtcatg gtggtgcatc
     1681 cctcggtacc cgccgcgacg gtgaaggagt tcgtctccct cgccaaggcg cagcccggca
     1741 agctcaacta cgcgtcttcc ggccccggca cgccgtacca catggccggc gagctcttca
     1801 aggcgatggc gggcgtggac gtggtgcacg taccctacaa gggcagctcc ggcgcgcgga
     1861 ccgacatcct gggcggccag gtgcagatga tgttcgacgc catcaccacc atggcgccgc
     1921 aggttcgcgc cgggaagctg agggctcttg gcaccaccgg caaggtgcgc tcgtcggtgc
     1981 ttcccgaggt ccccacggtg agcgaagcgg gcgtcgccgg gtacgagacg gtgatctggc
     2041 tgggcatcct cgcgccggcc ggaacgccca gggcgatcgt ggagcggctc aattccgaga
     2101 tcacgaagat cacggcgatg cccgagatgc gcgaggcctg ggcgaagcag ggcgccaccg
     2161 cgatgtcgat gacgacggag gagttcggcc gctacctgcg cgaggacatc gagaagtggt
     2221 cgcgcatagt gaagatttcg ggcgcgaagg ccgaccgctg acgtgaagct ggacatcctg
     2281 agcgccggcg cggcgcaggg cctggcgggc gccgtcgcga agcaggccgg cgtcgagctt
     2341 gccggcacct tcggggcggt gggcggcatc ctggaaaggt atctcgcggg cgaggcctgc
     2401 gacgtcgtca tcctcactca cgcgcaagtg gcccagctca ccgcgcgcaa tcgggtggct
     2461 ctcgacttct gctcggatct cggcgtggtc cgcacctcga ttgccgtgcg caagggtgac
     2521 gcgaagcccg acgtttccac gccgtccgcg ttgcgtgcag cccttctttc ggccgacgcg
     2581 atcttccttc ccgatccgca caaggctact gccggcattc acttcgcgag ggtgctcgcc
     2641 acgctgggca tcgcgggcga ggtcgcggcg cggctgcggc cgcacgccaa cggcatgacc
     2701 gcgatgcgtg cgctttccga agccggtggc cacccgattg gctgcacgca gaccaccgag
     2761 atcgtagcca cgcccggagt ggctctcgtg gcgccgttgc cgaaagagca cgagctggcg
     2821 acggtgtaca cggtggcact caagtccgga ccggcgagcg ccgcggcccg cgacttcgcg
     2881 cagcttctca ccggccccgc gaccgccgcc gcacgggctg ccgcgggctt cgaaggcgcc
     2941 gccatccggc gcgcgacggc tgcggatgcg accgccgcgc gcgaactcgt tttcggggtg
     3001 ctcgcggagt acgggttgac ccccgacccc aacgacaccg atgcggacct gatggacctc
     3061 gacgcgcact acctgtcgcg cggcggcagt ttcgacgttg ccattgcgcc cgatggacgc
     3121 ctcgttgcct gctgcgggtt gcggccgatg gccgggggtg cggtcgaact gcgcaagatg
     3181 tacgcgcgcc gggaagcccg cggccagggt atcggccggc gcctgctcga ccgtgcgctc
     3241 gccgtcgcgc gcgccagcgg cagccctcgc gtcgagctcg aaaccgcctc ggtgctgaag
     3301 gaagcgatcg ccctctaccg caaggcgggg ttcgtgccgc gccccggcaa gcccgacacc
     3361 cgccgctgcg acctcgcgtt cacgctggac ctgtcctgac cttgccgatc ctcgccaacc
     3421 tcaccaagtg ccgcgtgccg gtgaaggcat ggacggacga tctcgagccg caggcgcgcc
     3481 gccagctcga gaacgtggcg gaccttccga tcgtcttcgg ccatgtggcc gccatgccgg
     3541 acgtgcatgc gggaatcggt gccacggtcg gcagcgtcat tcccacgaag gcagccatca
     3601 tccccgcggc ggtgggcgtc gacatcggct gcggcatgaa cgcggtgcgg ctctccctcg
     3661 ccgccgccga cctcccgcag aatctcggac gcgtccgttc ggccatcgag gccgccgtac
     3721 ccgtcgggtt cgaccagcac ggcgagtgtc ccgtggagga cgccggatcg ctgcgcgcga
     3781 tgtcgcgccg gctggacgcc atcgccagga agcaccccgg actcacgaaa ctgcagcgga
     3841 gcttcgacac gacatgggcg cggcagctcg gcaccctggg cggcggcaac cacttcatcg
     3901 agctgtgcct ggacgaatcg atgcgcgtgt gggtgatgct gcattccggc tcgcgcggtg
     3961 tcggcaacgc catgggccgc tacttcatcg agcgggcccg gcgcgaggcg gaaaggctgg
     4021 accggcgcct tccggaccgc gacctcgcct ggctggacga gggcacgccc ctcttcgccg
     4081 actatgtcga ggcggtgggc tgggcgcagg actacgcgct ggccaaccgg cgcgagatga
     4141 tgctcctcat cgtggaagcc ctgcgccgcc acctgcctgc gttctcgatc gcgaacgagg
     4201 ccatcaattg ccatcacaac tacgtggccc gcgagcgcca cctcggcgag gatctcttca
     4261 tcacgcgcaa gggtgccatc cgcgccgggc tcggcgagct gggcatcatc cccggcagca
     4321 tgggcgcgcg ctcgttcatc gtgcgcgggc tgggcaacga gcagtcgttc tgctcctgct
     4381 cgcacggcgc ggggcgccgc atgagccgca ccgaggccaa gcgccgcttc agccgccagg
     4441 acctcgagcg gcagaccgaa ggcgtggagt gccgcaagga cggcggggtg atcgacgaga
     4501 tcccgggagc ctacaaggac gtcggcctgg tgatggcgca gcagtccgac ctcgtcgagg
     4561 tggtgcacac gctcaagcag gtgatctgcg tgaagggctg aacgcccgtt tcagcgatcg
     4621 cggcgcacgg tcatcatcgg cggcggcatg ccctcggccg gcggtttcgc ggtcgcggac
     4681 gaatcggcca ggccgttgcg gctcgcgctg aactccactt cgcaatcggg ccgctttccg
     4741 cacatgacgt ccctggcgag gatgcctacg cgcgccatgt cgacgggccg cgagtacagg
     4801 gggatcgcga tggtctcgcc gtacagcgta tcgagagcgt cgaggatgtc ggcggcaagc
     4861 cgcggcatgg gggaagagtc cccgggcagg gatggcgccg tcacgtttcc gtcgggcgcc
     4921 tcgacccgct ggcacaggac ggcctcgacc tccgtggacg cataggcaca atgccagcgc
     4981 acgacggggc cggccgaggc cagcgtcgaa accaggcagc agaaagcaag cgctgaaacc
     5041 cgcaattcca tgacacctcc ctgtgcgcgg cgtaatgcca gctacaggtt agggagtccg
     5101 gattgcgaac catagccaca gccaatttca ggcgtagcag cgacgacaga ttgcagcccc
     5161 agcccgttac cggcgtagcg ggggcggcaa tctgcgcccg cagcgatttc aggtgtagcg
     5221 cgagccaatc tccgtgaaac gagcccggtg cgcgccaatg cggggattac ttcaccgcgc
     5281 cgacgtcctt catcgactcg agcttcgcga ggtcgatgga ccccacggcc gaccggagcg
     5341 tctcgatcgg cacgccctgg ccgcgggcgc tcaccacgaa ccggtcgccc accaccacgg
     5401 tgtattcgct gtcgccgccg cgtttcgaga tttcctcgtg aaccatgcgc ttgccgtcgc
     5461 ggcgcgtgcg ctcgatgcgg tcgtcggttt ccttctcgcc ctggatgttc atccagcccg
     5521 ccaggcccat gaggccgccg gcgccgccgg tgtccgtcac ttccaggtcg acgtgcctgc
     5581 ccgcgccgtc gtcgtactgc gcctcggcct tcgagacctg caggcccgcc atgccgctct
     5641 tttccgcgct ctgactggta cgcggcaggt cggccagcct ttcgggcacc aggggtttca
     5701 actgctcgat gcccaggggc tcgaagcgct tgccgccgcc caggaccgtg cccagcccct
     5761 ccatcgccgc cttcatctgc gcgttggggt cgccgctctt ctgcgccgcc tccatcttcc
     5821 tgcccgcttc ctccatctgc ttgccgaatc cctcgagctt gcccatcggc gaatccttgt
     5881 cgaaagtcat gcccggggac ctgccgtgca tggcacccat catcgcgccg ccgccggtga
     5941 gcgcggcgag accgcccgcc atgagcgaga tcacgaaacc gatcacgatg gcgcagatga
     6001 cgacgacggc cgtgtagccg atggacttct cctcggggtt cttcatcagg cgcggcagcc
     6061 ccaggtagag gaggtagagg ccgtagagcg agacgagaac gccaatgagg cccagcgccg
     6121 gaatgatgcc gaggatgccg gcaatccagg ccggcgtgta ggaatagacc gccactttca
     6181 gcgcctgcgc cgggttcttc tcgccgccga aggtgggcgc cagcgcgttg atgatgagcg
     6241 acaggatgaa gatgccgacg aaggccatca cgaacgagaa cacggcgacg ccgatgcccg
     6301 cgaggatggg cgaacggtaa gtgccgatga agggcaggct cacgccgacg atggaactgc
     6361 cgacgaaccc gcacagcacg ctgatccccg cgagcggcgc gacgtatccc cccatgagat
     6421 cgccggtgga cgaggtttcg cctgcgatga tcggccactc ggactttggc gtgaggatga
     6481 tgttcttgac gcggtcgacg atgcccatga agcggctccc tgttggtatt gtgtgcattc
     6541 gattttacgc tccacgggga gttcggcatg gcgtcacgcg cggcatcggt tccgctgttc
     6601 gtactcgcgc tcgggcttgc cgcgtgttcc ggcggcggtg gcggtggttc tgcgccggtc
     6661 gcgagcctgc cgccgctgca acccgtcacg agcaccgtct ccggagtttc gccctttgcg
     6721 gccggatgcg gcgatgcgaa cggcaccttg ttcgtgaatg ccgaggtgga gccgagcctc
     6781 gcggccgatc cggccaaccc cggccacctc ctcgcgatgt ggcaacagga ccggtggtcc
     6841 aacggctcgg cacgcgggct cgtctcggcc gtctccttcg acggcggcgc cacttggagc
     6901 aggggagcga tggccttttc gcgctgcggc ggcggcaccg cggccaacgg gggcgacttc
     6961 gaccgcgcga ccgatccgtg ggtcgacttc gggcccggcg gcactgcgtg ggcgatgtcg
     7021 ctgtcgacca ctggtgcgtc gtccgccccg ggctcgacca acgcgatgct ggtcgcgcgt
     7081 tccgcggacg gcggccgcac ctggggcaat tccgtgccgc tgatcgccga taacgagctc
     7141 ttcttcaacg acaagaacgc gctcaccgcc gaccccaacg acgcccgctt cgcctacgcg
     7201 gtgtgggacc ggcttggaca atccggggga ggaccggcca tgttcgcccg caccgtcgac
     7261 ggcggaaact cctgggagcc ggcgcgcatc ctcttcgacc cgggcaccgc ggcgcagacc
     7321 atcggcaacc tcgtcgtggt cgtcaccggc ggcgcgctcg tcaacgtgac gacgagaatc
     7381 gaagagccgg cgggaaggcc gcgcacggcg tcgatcatcg cgcttcgctc cgtggatcgc
     7441 gggacgacct ggtcggatcc ggttcaggtc gccacgcacc ttgccgtcgg cgcgcgcgac
     7501 cccgagacag gcgcggcaat tcgcgacggc gcaatcctgc tcaatgcggc ggccggcccc
     7561 ggcggaacgc tgcacgtggt gtggcaggac tcgcgtttca cgcgcggctt gcgcgacgcc
     7621 atcgcgtatt cgcgctcgga cgacggcggc cttacgtggt ccgcgcccgt tcgcgtgagc
     7681 ccggacgcga cggtgtccgc gttcacgccc accgtgcgcg tgctcgccga cgcgacgatc
     7741 ggggtgacct acttcgacct gcgcgacaac acggccagcc ccgccacgct ccccgccggc
     7801 tactggctcg cgcggtcggc cgacggggtg acgtggacgg aaacgcgcct cgccgggccc
     7861 ttcgacctgg ccaccgcccc gaacgcgggg ggcctctttc tcggcgacta catggggctc
     7921 gcgggcagcg gctcggcctt cctgtctctc ttcgtgcgca ccacgggaga cctcgccaac
     7981 cgcaacgacg tcttcttcgt gcgcgcggcg gcgacatccg cggccatcgc gcaaggcaag
     8041 ggcatcgcgt ccgatggcgc gtggcccggt cgctgggtcg cgaaggccgc agcgccgttc
     8101 gaagtggacg ccgcgtgggg cgcgcgcgtg gacgcggcga tcgcgcgggc gctggcggcg
     8161 cgcgggcggc cgtcgccttc gcgctagcta ttcgtccggc gccaccgagc ggatcgtgta
     8221 gccgccgctc ttctcgtcgc gttcgagctc gacgaggccg cgttcctgga gctcctcgag
     8281 cagctggttg aaggagcggt agccgtagta gctctcgctg aagccgggct tcctgcgctt
     8341 gatggcctgc ttcaccatcg atccccagag cgtttcctcc acgccgcgct cgtcgaccag
     8401 gtcgtcgatc gtctcgagca ccagctcgat ggcctcctgc ttcttgccgg tgcccccgcc
     8461 gttttcacgc tccttttcct tgtccttgac gggtgcggcc ggcttgccct ccaccttctt
     8521 cgcggactgg cgcttcgccg ggtgctcgcg caccaggtcg tcgtagtaga tgaactcgtc
     8581 gcagttcgcg atcaggaggt cggaggtgct cttcttcacc ccgatgccga tcacggtctt
     8641 cgcgttctcg cgcagtttcg agacgagcgg ggaaaagtcg gaatccccgc tcacgatcac
     8701 gaaggtgtcc acgtggctct tggtgtagca gaggtccagg gcgtccacca ccatgcggat
     8761 gtcggcggag ttcttgccgg actggcgcac gtgcgggatc tcgatcagct cgaaggaggc
     8821 ctggtgcatc tgcgccttga attccttgta gcgctcccag tcgcaatagg ccttcttcac
     8881 cacgatgctg cccttcacca gcaggcgctc gagcaccttg ttgatgtcga acttgtcgta
     8941 tttcgcctcg cgaacgccga gcgcgacgtt ctcgaagtcg cagaagacgg caaggttgcg
     9001 ggtctccgcc atgggcacct ccctgggaat gtcccctagc ttacaatgcc gccatgctat
     9061 ccgccggcag cgacaacctg atcgagatcc gcgacctgca cttcgcctac ggcaagcgcc
     9121 cggtgctgaa ggggctcgag ctcgacattc cgaagggcaa ggtcgtcgcg atcctgggtg
     9181 cgagcggctg cggcaagacc acgctgctgc agttgatcgg gggcgcgctg gcgccttcgc
     9241 gcggcagcgt caaggtgcac ggcctggagg tgcacacgct ggaccacgag gggctgtacc
     9301 gcctgcgccg gcagatcggc atgatgttcc agaaaggcgg gctgttcagc gacctgacgg
     9361 tcttcgagaa catcgccttc ccgatgcgcg agcacacctc gcttccggac gagatcattt
     9421 ccgacctcgt gaagatgaag ctgaacgcgg tggggctgcg cggcgcgcac cggctctacc
     9481 ccacggaact ctccggcggc atggcccgcc gggtcgcgct ggcccgcgcc atcgcgctcg
     9541 accccgacgt tctcatctac gacgagccct tcgcgggcct cgacccgatc acgctcaacg
     9601 tgatctgcaa cctgatccgc gcgctgaacg acgcgctggg cagcacctcc gtcgtcgtca
     9661 cttacgacgt gccggaggcg gtgaagctcg ccgactacct ctacgtgatg ggcgacggac
     9721 gcatcgtcgg ccacggtccc acgcaggaga tgctcgcctc cgacgacccg ttcgtgcgcc
     9781 agttcctgca cgccgagacc gacggtccgg tgcccttcca cttccctgca aggccgatcg
     9841 ccgaggaact ggagctcgcg ccggcgcgca ggcccgcccg ctcggttcgc gccctctagc
     9901 gccatcgcgc agacccgccg ccacgactga acgttaccga ccaaaccccg gaggaaccat
     9961 gatccaccgt ctcgccgggg ccgcttgcgc ggccctcgcc ttcgcccttc ccgccctcgc
    10021 ccagaccgac taccccacgc gaacgatcac catgatcgtg ccgttcccgc cgggcggcgt
    10081 ggccgacatc accgcgcgtc ccgccgccga ggcgatgggc cgctacctca agcagacggt
    10141 catcgtcgag aacaagtccg gggcgggcgg gggtgtgggc atgcaatacg tttcgcgcgc
    10201 caagcccgac ggctatacgc tgctgctcgc gctctcctcg atctccatca tcccggaagc
    10261 cgacaggatc ctcgggcgcg acccgatgta ccagctgtcg cagctcgtcc ccatcgcgcg
    10321 tttcaccgcg gatccgacgg tgctcgcggt gcgcgccgac agcccgtgga agagcgccag
    10381 ggagatgctg gaggccgcga agaaggcccc gggaaccatc ccctacggct cgtcgggaaa
    10441 ctacggcacg atgcacgtgc cgatggagat gctcacgggt gccgccggcg ccaagatgct
    10501 gcacgtgcct ttcaccggcg ccggcccggc cgtcgtggcg ctcctcggcg gcaacgtgga
    10561 tgcgctgtcc acgggcccct cgtcgatcat gggccacctg aagggcggca agttgcgcgt
    10621 gctcgcgagc tggggcgaaa cccgccaccc cgcgctcccc gacgtgccaa cgctcaagga
    10681 actgggctac gacgcgacgt tctcgcagtg gacgggcctc ttcgtgccgg ccggaacgcc
    10741 ggaagcggtg atcgcgaagc tgcgcgaagc cgccagggcc acggcatccg accctacatt
    10801 cctcgcctcg ctcgcccgcg tcgaaacgcc gctgcaatac ctcgaccagc cgcagttccg
    10861 caatttctgg gacgcggacg cgaagaagct ctccgacgtc gtgaagaaga tcggcaaggt
    10921 cgaatgacga ttcgagatcg ggagatgcga ccccaatagc tggaagacgc ggcgaattgg
    10981 aggtgggccg gggatggggc aaatctatcg gcgtcctggg cacgcaagcg tgaccgtcgg
    11041 atgggtccca aaaatccgag gccaggcaga acaagcccgg cctcggattt tttgcccatt
    11101 gagagatggt gccgctgcgc tctcccatcc cgacggt
//
LOCUS       DASUEV010000042         6253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_13101, whole genome shotgun sequence.
ACCESSION   DASUEV010000042 DASUEV010000000
VERSION     DASUEV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13101"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFV55_01420"
     CDS             <1..261
                     /locus_tag="VFV55_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942978.1"
                     /translation="RRQRSAFSWPVNIGRLLRLICTTPLKVIQNNGVLHRPIESAGQN
                     QKTRTNQPDPNKPLRSPPCRFHAIDHRRGEFTRSITAVPNSR"
     gene            complement(274..975)
                     /locus_tag="VFV55_01425"
     CDS             complement(274..975)
                     /locus_tag="VFV55_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942979.1"
                     /translation="MERRDFVKVCAASAATATFPTAADAATLKAQFYRRALLVDERDQ
                     PIRPEALRVGVNYVFDYPFAATPCFLLRLAKPATGGIDLRTEKGGTYRWEGGVGPGKA
                     IVAYSAICAHKMTYPTRQVSFIGYRDGPSPVAGPGKVIACCSDRSVYDPSAGARVVSG
                     PAPQPLATILLEHDPKAGELTAVGTFGGEMFGQFFQKFGFRLELEMGDRAKASTDARA
                     VVRELANYSTQWAQC"
     gene            complement(1145..4213)
                     /locus_tag="VFV55_01430"
     CDS             complement(1145..4213)
                     /locus_tag="VFV55_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4942980.1"
                     /translation="MVHRFNVSEWALAHQTLVLYLMVVLAAIGIGSYLRLGQAEDPDF
                     TFKLMVVKTNWPGASADEVERQLTDRIEKKLQETPRLDHLRSYSKPGESVVFVFVKDS
                     SRPDEIRETWYQVRKKVGDIRSQLPPGIQGPFFNDEFGDTFGNIYALTGDGFSYAQLK
                     EAADRIRADLLRVTDVAKVELIGEQDEKIFVEISNAKLATSGVEPAVVFAALAQQNAV
                     ASSGSFETATDRLYIRTSGALDSVESVRELPIRANGRLFRVGDLATVTRGFSDPPQPK
                     MRFMGRDALGVAVSMAPRGDIVALGHALDAEVERIKAELPVGIELDRVNDQPAAVTRS
                     VREFTRTLAEAVAIVLLVSFLSLGLRTGLIVALSIPLTFAVTFLFMYQSGIDLHKVSL
                     GALIIALGLLVDDAIISVEMMVVKMEQGWERVRAASFAYTSTAMPMLTGTVVTAAGFL
                     PIGLARSSTGEYTFAMFAVTTIALLVSWVVSVLFVPYLGYKLLPDFHRAGAHADEGAV
                     YRKPFYLRFRRLVNWSVAHRWTVIGSTLAVFALSVFAFRFVQQQFFPPANRPELIVDL
                     RLAEGSSFTATQAQVARLERILMTEPALKDNIDNFVSYVGSGSPRFFLPLDQQLVNAN
                     FGQFVVNTKNNESREIVRARLLELFDGDFAEIRGRVTRLENGPPVGFPVQFRVIGEDK
                     EAIRAIANEVSGVMRANPWTREVNVDWEEASRVIRLAIDQDRARVLGISTQELAQYVA
                     TVISGQAVTSWRENDKQVDVVVRGNAAERQMVSLLKDLAVPIGRGRTVPLAQIARLDY
                     GFEQGIYWRRSRVPVITVRADIRDGIQAPAVSAQVNEKLDAIRAKLPFGYRIETGGAI
                     EDAGKGQRSIAVVAPVMILVMLTTLMLQLQSFSRVTMVALTAPLGLIGVTAALLVSRM
                     PFGFVAMLGTIALAGMIMRNSVILVDQIDHDIREGSQPWDAIVDATVRRFRPIMLTAA
                     AAVLAMIPLTRSAFYGPMAVAIMGGLLVATVLTLVFLPALYAAWFRVKPA"
     gene            complement(4224..5327)
                     /locus_tag="VFV55_01435"
     CDS             complement(4224..5327)
                     /locus_tag="VFV55_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4942981.1"
                     /translation="MNRALPLLAALFISACGNGHQAEDPIRPVLTVTVAPGAAATREV
                     YSGELRARVETDLAFRIGGKIATRLVDAGAHVKKGQVLARLDPQDAKLAAEGARAQLA
                     SAEADHALAKSEFDRAGDLLARKFISQSAWDARQAAYTAAKARVDQARSQAALSTNQA
                     AYTTLAADADGVVVSVAAEPGQVVAAGQPVLRLARDGEMEVVVNAPESQVARFRPGQD
                     VVVFLWADPANRFPGRVREIAGGADPVTRTFTVRVSAPRVPPGARVGMSATVAFKAET
                     DAGLVVLPLTALVREADSATVWVVDPKTSRVQRRAVTVGQFREDGVTVLSGLAAGEIV
                     VSAGVHKLRADQAVRFAAARSPDAHRPDASAVR"
     gene            complement(5324..6004)
                     /locus_tag="VFV55_01440"
     CDS             complement(5324..6004)
                     /locus_tag="VFV55_01440"
                     /inference="COORDINATES: protein motif:HMM:NF037677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX4942982.1"
                     /translation="MAIKQRAIAVEDKEERRNALLDAAEALFLDHPDRMASVAEVAGT
                     AGVAKGTVYLYFPSKEEMLLSLHERHMARFFAALMKLLDGRKAAGFDEVWATTRKNLV
                     RLPGYLPLTGRCLGLMDRDIPTEAAVAFKIRVGQALSAAGTGLERHFPALGTGGGVTL
                     LQHSYGLIVGLWQLMHPIERFGTAMERAELAMFKRDYERELEQALRALWAGTLGVGFA
                     LRKKRRSR"
BASE COUNT         1035 a         2051 c         2204 g          963 t
ORIGIN      
        1 cggcgccaaa gatctgcctt ctcctggccc gtcaacatcg gccgattgct tcgcctcata
       61 tgcaccaccc ccttgaaagt gattcagaat aacggggtgt tgcatcgacc cattgaatcc
      121 gccggccaaa atcaaaaaac aagaaccaac caacctgacc cgaacaagcc tcttcgatca
      181 ccgccgtgcc gatttcacgc gatcgatcac cgccgtggcg aattcacgcg atcgatcacc
      241 gccgtgccga attcgcgttg acccggctcg ccgctagcac tgcgcccact gggtgctgta
      301 gttcgccagt tcacgaacga ccgcgcgcgc gtcggtcgag gccttcgcgc ggtcgcccat
      361 ctcgagctcc aggcggaagc cgaacttctg gaagaactgc ccgaacatct cgccgccgaa
      421 cgtgcccacg gcggtgagct cgccggcttt cgggtcgtgc tcgaggagga tggtggcaag
      481 cggctgcggc gccgggcccg acacgacgcg tgcgccggcg gaggggtcgt agacgctgcg
      541 atcggagcag caggcgatta ccttgccggg accggcgacg gggctggggc cgtcgcggta
      601 gccgatgaag ctcacctgcc gcgtcgggta ggtcatcttg tgcgcgcaga tggcggagta
      661 ggcgacgatc gccttgcctg ggccgacgcc gccctcccag cgataggtgc cgcccttctc
      721 ggtcctcagg tcgatgccgc cggtcgcggg cttcgcgagg cgcagcagga agcagggggt
      781 cgcggcgaag gggtaatcga acacgtaatt gacgccgacc cgcagtgcct ccggtcgaat
      841 cggctgatcc cgttcgtcca cgaggagcgc ccgccggtag aactgcgcct tgagggtggc
      901 ggcatcggcg gcggtgggaa aggtcgcggt cgcggcggag gcggcgcaga ccttcacgaa
      961 gtcccggcgt tccatcggcg gaagatagca tgtgaagagg ggtcaggtta ctttgcacaa
     1021 gaaataggca agaacggaaa aggggtcgaa aaggggtcag gttacttttc tgtcgtattg
     1081 ccgtcgctag acggcgggct gaaaagtaac ctgaccccgt ttcgacccct tttcgactcc
     1141 cttttcaggc gggcttcacg cggaaccagg cggcgtagag cgcgggcagg aagacgagcg
     1201 tgagcacggt ggccaccagg agcccgccca tgatcgcgac ggccatcggc ccgtagaacg
     1261 cgctgcgcgt gagcgggatc atggcgagca ccgccgccgc cgcggtgagc atgatcggcc
     1321 ggaagcggcg cacggtcgcg tccacgatgg catcccaggg ttggctgcct tcgcggatat
     1381 cgtggtcgat ctggtcgacg aggatgaccg aattgcgcat gatcatcccg gccagcgcga
     1441 tggtgcccag catggcgacg aaaccgaagg gcatccgcga gacgaggagg gccgcggtca
     1501 ccccgatgag ccccagaggc gcggtgagtg ccaccatggt cacgcgcgaa aagctctgca
     1561 actgcagcat gagagtggtg agcatgacca gtatcatcac cggcgccacc accgcgatcg
     1621 agcgctggcc cttgcccgcg tcctcgatgg cgccgccggt ctcgatacgg tagccgaacg
     1681 ggagcttcgc ccggatcgca tcgagctttt cgttcacctg ggcgctcacg gcgggggcct
     1741 ggatgccgtc gcggatgtcg gcgcgcacgg tgatcaccgg tacacggctt cgccgccagt
     1801 agatgccctg ctcgaacccg tagtcgaggc gcgcgatctg ggcgagcggc accgtccgcc
     1861 cgcgaccgat gggcaccgcg aggtccttga gaaggctcac catctggcgc tcggccgcgt
     1921 tgccgcgcac cacgacatcc acctgcttgt cgttctcgcg ccaggacgtg acggcctgcc
     1981 ccgagatcac cgtcgcgacg tactgcgcga gctcctgcgt cgagatgccg agcacgcgcg
     2041 cgcggtcctg gtcgatggcc aggcggatga cgcggctcgc ctcttcccag tcgacgttca
     2101 cctcgcgcgt ccacgggttg gcgcgcatca cgccggatac ctcgttcgcg atggcgcgga
     2161 tcgcctcctt gtcctcgcca atcacgcgga actggaccgg gaagcccacg ggcgggccgt
     2221 tctcgaggcg cgtcacgcgg ccgcgaatct cggcgaaatc cccgtcgaaa agctcgagca
     2281 ggcgggcgcg cacgatctcg cgggactcgt tgttcttcgt gttgaccacg aactggccga
     2341 agttggcgtt gacgagctgc tggtcgagcg ggagaaagaa gcgcgggctg ccgctgccca
     2401 cgtagctcac gaagttgtcg atgttgtcct tgagcgcggg ctccgtcatc aggatccgct
     2461 cgagccgggc cacctgggcc tgggtcgcgg tgaaggatga gccctccgca agccgcaggt
     2521 ccacgatcag ctcgggacgg ttggccggcg ggaagaactg ctgctgcacg aagcggaagg
     2581 cgaaaacgga aagggcgaac accgccagcg tgctgccgat caccgtccag cggtgcgcca
     2641 cgctccagtt caccagccgg cggaagcgca aatagaaggg cttgcggtag acggcgcctt
     2701 cgtcggcgtg cgcgcccgcc cggtggaaat ccggcagcag cttgtagccc aggtacggga
     2761 cgaagagcac cgagaccacc caggacacga ggagcgcaat ggtggtcacc gcgaacatcg
     2821 cgaaggtgta ctcgcccgtc gaggaacgcg cgaggccgat gggcaggaac ccggcggccg
     2881 tcaccacggt gccggtgagc atcggcatcg cggtggaggt gtaggcgaag ctcgcggcgc
     2941 gcacgcgctc ccagccctgt tccatcttca ccaccatcat ctccaccgag atgatcgcgt
     3001 cgtccaccag caggccgagc gcgatgatga gcgcgcccag cgacaccttg tgcaggtcga
     3061 tgcccgactg gtacatgaag aggaaggtga cggcgaaggt gagggggatg gagagcgcca
     3121 cgatgagccc cgtcctcagc cccagcgaca gaaagctcac caggagcacg atggcgacgg
     3181 cttccgcaag cgtgcgcgtg aattcgcgca ccgaacgcgt caccgcggcg ggctggtcgt
     3241 tgacccggtc gagctcgatg cccacgggaa gctccgcctt gatccgctcg acctcggcgt
     3301 cgagcgcgtg gcccagggcg acgatgtcgc ctcgcggcgc catcgagacc gcaacgccca
     3361 gcgcgtcgcg gcccatgaag cgcatcttcg gctgcggcgg gtcggagaac ccgcgcgtga
     3421 cggtggcgag gtcgcccacg cggaagagcc gcccgttggc gcggatggga agctcgcgca
     3481 cgctttccac cgagtcgagc gcgccgctgg tgcggatgta gaggcggtcg gtggcggtct
     3541 cgaagcttcc cgacgaggcc acggcgttct gctgcgccag cgcggcgaag acgacggcgg
     3601 gctccacgcc cgaggtcgcg agcttggcgt tggagatctc gacgaagatc ttctcgtcct
     3661 gctcgccgat cagctcgacc ttggccacgt ccgtcacgcg cagcaggtcg gcgcggatgc
     3721 ggtcggcggc ctccttgagt tgcgcgtagc tgaagccgtc gccggtgagc gcgtagatgt
     3781 tgccgaaggt gtcgccgaac tcgtcgttga agaaggggcc ctgtattccc ggcggaagct
     3841 ggctgcggat gtcgcccacc ttcttgcgca cctggtacca ggtttccctg atctcgtcgg
     3901 gccgcgagga gtccttcacg aacacgaaga ccaccgattc gccgggcttc gagtagctgc
     3961 gcaggtggtc gaggcgcggc gtctcctgca gcttcttctc gatgcggtcg gtgagctggc
     4021 gctcgacctc gtcggcggag gcgccgggcc agttcgtctt cacgaccatc agcttgaagg
     4081 tgaagtccgg atcctcggcc tggccgaggc gcaggtacga gccgatgccg atggcagcga
     4141 ggacgaccat gaggtagagc accagcgtct ggtgcgcgag cgcccattcc gagacgttga
     4201 agcgatggac catgggcggg ccttcagcgc acggcggacg cgtcggggcg atgcgcgtcg
     4261 ggggaacgcg cggcggcgaa gcgaaccgcc tggtcggcgc gcagcttgtg cacgcccgcc
     4321 gagaccacga tctcgcccgc ggcgaggccc gagaggacgg ttaccccgtc ctcgcggaac
     4381 tggcccacgg tcaccgcgcg ccgctgcacg cgggacgtct tcggatcgac gacccacacg
     4441 gtggcggagt ccgcctcgcg cacgagtgcc gtgagtggaa ggacgacgag gccggcgtct
     4501 gtctccgcct tgaaggccac ggtggcgctc atgcccacgc gagcgccggg cggtacgcgt
     4561 ggcgcggaaa cgcgcaccgt gaacgtccgg gtcacggggt cggcaccgcc ggcgatctcg
     4621 cgcacgcggc ccgggaaccg gttggcggga tcggcccaca ggaagaccac cacgtcctgg
     4681 cccgggcgga aacgcgcgac ctggctttcc ggggcgttca cgacaacttc catctcgccg
     4741 tctcgcgcca gccgcaggac gggctggccg gcggccacga cctgcccggg ctcggcagcg
     4801 acggagacca cgacgccatc ggcatccgcg gcgagcgtcg tgtaggccgc ctgattggtg
     4861 gaaagtgccg cctgcgagcg tgcctggtcc acgcgggctt tcgcggccgt gtacgcggcc
     4921 tgccgcgcgt cccaggcgga ctggctgatg aatttcctcg cgagcaggtc gccggcccgg
     4981 tcgaactcgg acttggcgag cgcgtgatcg gcctccgccg aggcgagctg cgcgcgggca
     5041 ccttccgccg cgagcttcgc atcctgggga tcgaggcgtg cgagcacctg ccccttcttc
     5101 acgtgcgcgc cggcatccac cagccgagtg gcgatcttgc cgccgatgcg aaaggcgagg
     5161 tcggtctcca cgcgcgcgcg cagttcgccc gaatacacct cccgcgtcgc ggcggccccc
     5221 ggggcaacgg tgaccgtgag caccggccgg atcgggtcct cggcctggtg gccattgccg
     5281 caggccgaaa tgaacagggc cgcgagcagg gggagggctc ggttcatcgg gatctccttt
     5341 tcttgcgcag cgcgaacccg acgcccaggg tgccggccca cagcgcgcgc agcgcctgct
     5401 cgagctcgcg ttcgtagtcg cgcttgaaca tggcgagttc ggcgcgctcc atggcggtgc
     5461 cgaagcgttc gatcgggtgc atgagttgcc acagccccac gatgaggccg tagctgtgct
     5521 gcaggagcgt gacgccgccg cccgttccga gcgccgggaa gtggcgctcg aggccggtgc
     5581 ccgctgcgga aagcgcctgc ccgacccgga tcttgaaggc gacggccgcc tccgtcggga
     5641 tgtcgcggtc catgagcccg aggcaacggc cggtgagcgg gaggtagccg ggcagccgca
     5701 cgaggttctt gcgtgtcgtg gcccagacct cgtcgaagcc agccgccttt cgcccgtcga
     5761 ggagcttcat gagcgcggcg aagaagcgcg ccatgtggcg ctcgtgcagc gacaggagca
     5821 tctcttcctt gctggggaag tagaggtaga ccgttccctt cgccacgccc gcggtcccgg
     5881 cgacctcggc cacgctcgcc atgcggtcgg ggtgatcgag gaacagcgcc tccgcggcat
     5941 ccaggagggc gttgcgccgc tcctccttgt cctcgacggc gatggcgcgc tgcttgatgg
     6001 ccataaatga ctccaggtca gttggcggca aaataactga ccgacggtca ggagtcaagg
     6061 cgtcctgcgt tacgctttgt tctcgcgata gcgtggtggg agcgcgagct tgaccggcga
     6121 aggggagtcg ggccggggat ggggcaagtt aatcggcgtc ctgggcacgg cgaaggggag
     6181 tcgggcctgg gatggggcaa gtctatcggc gtcctgggca cgcaagcgtg accgtcggat
     6241 gggtcccaaa aag
//
LOCUS       DASUEV010000043         4740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1268124, whole genome shotgun sequence.
ACCESSION   DASUEV010000043 DASUEV010000000
VERSION     DASUEV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268124"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1122)
                     /locus_tag="VFV55_01445"
     CDS             complement(97..1122)
                     /locus_tag="VFV55_01445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX4942983.1"
                     /translation="MNDAPSTPPYAGLTPDVVLNAVESVGFLADGRQLALNSYENRVY
                     MVGLEEDQVGPLGDARFLVAKFYRPGRWSDAQILEEHEFARELAEREIPVVAALEIGG
                     TTLHHHGGFRFSLYPRRGGRSPELEDPYTLEWLGRFIGRIHAVGGTKPFVLRPTLDVE
                     AFGTASRDWLLAHDFLPPDLREAWSGAAALAIEGAQCAFARVAGAARIRLHGDCHAGN
                     ILWTPGGPHFVDFDDARTGPAVQDLWMLLSGDRSSMSAQLRHVLRGYEDFAQFDDHEL
                     GLVEALRTLRLLHYSAWLARRWGDPAFPMAFPWFNTQKYWQDRVLELKEQVAAMQEPP
                     LVPDRSG"
     gene            1278..2264
                     /locus_tag="VFV55_01450"
     CDS             1278..2264
                     /locus_tag="VFV55_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4942984.1"
                     /translation="MHSISPILRRLSRRTATPPGDATAWLDADESFALWGAVLDGALP
                     ELELGTLLASLAITGESAEELLGLHRALAARQARFSPALARRAVSIPVHGLVPGEAAF
                     SVLLALFLRRFEVPVVMHGPLDSPEGPSAPMLLRELGVMPSATLADAERDLRAGGIAF
                     IPVQILSPALGDILALRNRLGATNSAHLAAHAMDPGEMGAVRLAMSVPGTPSGRLPTF
                     LAASGGEALLLSWPATTLACTFAFRPRISHLSGGAEAVLFQAENLERAGQAGPPASAP
                     LVPWMRAVVERRAPAPPPLVNLAAACVHAAGAAADFTEAKAIVALQAGRLAA"
     gene            2523..2984
                     /locus_tag="VFV55_01455"
     CDS             2523..2984
                     /locus_tag="VFV55_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX4942985.1"
                     /translation="MAGVMWVGLLYYFNFVQAQAMKDAQADGTGAGISKHLAPRALLF
                     FRWAAVATWLAGTWLLGDKIDEAFLFKERAYIPIGVGAWLGTIMFINVWAIIWPNQKK
                     ILGLAPASEDEKTRARRVAFVASRLNVVLSMPMLFFMSASGGLFRTAVFGG"
     gene            2986..3681
                     /locus_tag="VFV55_01460"
     CDS             2986..3681
                     /locus_tag="VFV55_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX4942986.1"
                     /translation="MNDRARVSIRIWDLPTRLFHWALACLVIASVVTGKFNSILGPGS
                     LEWHKRSGLAILALLLFRFVWGLAGGTHARFANFLRGPRAVIAYARGLFAGDAASETA
                     GHNPLGGWSAVAMLAAIALQACAGLFLIQEDFAFEGPLAHLVSRAVSDRLGAIHQANF
                     YAIAVLVALHVAAIVFYTVVKKQDLVGAMVTGRKSLAPGTEGEASRGGGAAVAIAVAI
                     ASATAVWALVTFA"
     gene            complement(3682..4149)
                     /locus_tag="VFV55_01465"
     CDS             complement(3682..4149)
                     /locus_tag="VFV55_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4942987.1"
                     /translation="MKRFALAAAVLAATSAAVAFAQQGPKPEDEIRYRQSVMNVIGRA
                     MGPMGAMAQGKAAFNAAVVQKNSALIDSLIGLHWNSFGPGTDKGAPTKADPKVWSEAP
                     KFKKLAEDVQKAVAHLAAESKSGDEAKFKAAFGEVGKACKACHDDFRTKEFRS"
     gene            complement(4242..>4740)
                     /locus_tag="VFV55_01470"
     CDS             complement(4242..>4740)
                     /locus_tag="VFV55_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX4942988.1"
                     /translation="VRQINWRFFLEAILPALLPRSTAGRLVAAALFAAALPALAEAPL
                     PVSNVRVAGHALRVEVVATMEQRAKGLMFRKKLGKNDGMLFVFEEPAYHAMWMKNTLI
                     PLSVAFIDGEGRILNIADMEPETLDSHAAAGPARFAIETNKGWFAERKVVAGSKVTGL
                     PKLAK"
BASE COUNT          706 a         1565 c         1634 g          835 t
ORIGIN      
        1 attcgttggt gtcagaaacc ggacccgcca accctgagag gagtctgaca ccaacgaatg
       61 ggttgcttaa tttttgtgca aagtaacctg acccctttaa cccgagcgat cgggcacgag
      121 cggcggctcc tgcatcgcgg cgacctgttc cttcagttcc agcacgcggt cctgccagta
      181 cttctgcgtg ttgaaccacg ggaaggccat gggaaacgcg ggatcgcccc agcgccgcgc
      241 gagccacgcg gaatagtgca gcagccgcaa cgtgcgcagc gcctccacga ggcccagttc
      301 gtggtcgtcg aattgcgcga agtcctcgta gccgcgcagc acgtggcgaa gctgcgccga
      361 catcgacgaa cgatcgccgg agagcagcat ccagagatcc tggaccgcgg ggccggtgcg
      421 cgcatcgtcg aagtcgacga agtgggggcc gcccggcgtc cacaggatat tgccggcgtg
      481 gcagtcaccg tgcaggcgga tgcgcgcagc acccgccacg cgagcgaatg cgcattgcgc
      541 cccctcgatc gcgagggccg cggcgcccga ccaggcctcg cgcaggtccg gcgggagaaa
      601 gtcgtgcgcg agcaaccagt cgcgggaggc ggtgccgaac gcctcgacgt cgagcgttgg
      661 ccggagcacg aacggcttcg tgccgcccac ggcgtggatg cggccgatga accgccccag
      721 ccactcgagc gtgtagggat cctcgagttc cggcgaacgg ccgccgcggc gcggatagag
      781 ggagaagcgg aaccccccgt gatgatgcaa cgtggtaccg ccgatttcga gggcggccac
      841 caccgggatc tcgcgctcgg ccagctcgcg ggcgaactcg tgctcctcca ggatctgcgc
      901 gtctgaccat cgcccggggc ggtagaactt cgccacgagg aaccgggcat cgccgagggg
      961 gccgacctgg tcttcctcga gacccaccat gtagacgcgg ttctcgtagc tgttgagggc
     1021 gagctggcgg ccgtcggcca ggaagccgac gctctcgacg gcattgagca cgacgtcggg
     1081 cgtaaggccc gcgtagggtg gcgtcgaggg ggcatcgttc acccccgcat tgtacggccg
     1141 cccccgaatg ggcctgtggc ccgcggtatt gcacccgcgc ggtgcggttc gcatcggggt
     1201 cgcccctccg ccgggttgaa acgcgcgtgg catgcctcct gctaccagtt cggggcgaac
     1261 accgccggag ccttcccatg cactcgatat cgcccatcct tcgccgactc tcgaggcgga
     1321 ccgccacccc gcccggcgat gcgaccgcat ggctggatgc ggacgaatcc ttcgcactgt
     1381 ggggagcagt cctggacgga gcgctgccgg aactggaact gggtaccctg ctcgcgtcgc
     1441 tggcgatcac cggcgagtcc gcggaggaac ttctcggcct gcaccgggcg ctggcggcgc
     1501 ggcaggcgcg gttctcgccc gcgctggcgc ggcgcgcggt atcgatcccg gttcacgggc
     1561 tggttcccgg cgaggccgcg ttctcggtgc tcctggcgct tttcctgcgt cgcttcgagg
     1621 tgccggtggt gatgcatgga ccgctggatt cgccggaggg accttccgcg ccgatgctcc
     1681 tgcgcgagct gggcgtgatg cccagcgcga cgctcgcgga tgcggagcgc gaccttcgcg
     1741 ccggcggcat cgctttcatt ccggtgcaga tactctcgcc ggcgctgggc gacatcctcg
     1801 ccctgcgcaa ccggctgggg gccaccaact cggcacacct cgcggcgcac gcgatggacc
     1861 cgggggaaat gggagcggtg cgcctcgcga tgagcgtgcc gggaacgccg agcggacggc
     1921 tgccgacatt tctggccgct tcgggaggag aagcgttgct gctttcgtgg cccgcgacga
     1981 cgctcgcgtg cactttcgcg ttccggccgc gcatctcgca cctttccggc ggcgcggaag
     2041 cggtcctgtt ccaggccgaa aatctcgagc gcgccgggca ggcgggaccg cctgcgtccg
     2101 cgccgctcgt accctggatg cgcgccgtcg tcgagcgacg cgcgcccgcg ccccctccgc
     2161 tcgtcaacct cgctgcggcc tgtgtccatg ccgccggggc cgccgccgac tttaccgagg
     2221 ccaaggcgat cgtcgccctg caggccgggc gcctggccgc gtgatgaatg cgtaacccat
     2281 taatcctatt gggattttca ccacgttgcg aggcggcgac aggggcgcgt gtgctagaat
     2341 caagcccacg ccgtgccctt gcggaaacgg agcgccccga tgaaaaaccc tctcgattcc
     2401 ctgtggggca cagtcctctg cggactggcc ctgaccgccg ttctcttcta cctcgtgcgc
     2461 acgctgatgg gaggctgacc gtggatttcg tccattacgg gctgggtcgg tggctgcaca
     2521 tcatggccgg ggtgatgtgg gtgggactgc tttactactt caacttcgtg caggcgcagg
     2581 caatgaagga cgcgcaggcc gatggaaccg gtgccgggat ctcgaagcac cttgcgccgc
     2641 gggccctgct cttcttccgc tgggctgccg tcgcaacctg gctcgcgggc acgtggctcc
     2701 tgggcgacaa gatcgacgaa gcgttcctct tcaaggagcg cgcctacatc cccatcggcg
     2761 tcggcgcctg gctcggcacg atcatgttca tcaacgtgtg ggcgatcatc tggccgaacc
     2821 agaagaagat cctcgggctc gctcccgcct ccgaggacga gaagacgcgg gcgaggcgcg
     2881 tcgcgtttgt cgcctcgcgc ctgaacgtcg tgctgtccat gccgatgctg ttcttcatgt
     2941 ccgcctcggg agggctgttc cgcaccgccg tcttcggcgg ctgacatgaa cgatcgtgcg
     3001 cgcgtttcga tccgcatctg ggacctgccc acgcggcttt tccactgggc gcttgcctgt
     3061 ctcgtgatcg cgagcgtggt caccggcaag ttcaactcca tcctcggtcc cggttcgctc
     3121 gagtggcaca agcgctcggg gctcgccatc ctggcgctgc tgctgtttcg cttcgtctgg
     3181 ggacttgcgg gcggcacgca cgcgcgattc gcgaacttcc tgcgcggacc gcgggcggtg
     3241 atcgcctatg cgaggggcct cttcgcagga gacgcggctt ccgaaacggc cgggcacaat
     3301 ccgctggggg gatggtccgc tgtcgcgatg ctcgcggcga tcgcgctgca ggcctgcgcc
     3361 ggcctcttcc tcatccagga ggacttcgca ttcgaagggc cgctcgcgca tctcgtgtca
     3421 cgggcggtat cggaccggtt gggagccatc caccaggcga acttctacgc gatcgcagtg
     3481 ctcgtcgcat tgcacgtcgc ggcgatcgtg ttctacacgg tggtgaagaa gcaggacctg
     3541 gtgggtgcga tggtcacggg acgcaagagc ctggcgccgg gaacggaagg cgaggcgtcg
     3601 cgtggtggcg gcgctgcggt ggccatcgcg gtcgcgattg cgagcgcgac ggccgtgtgg
     3661 gcgctggtta cgttcgcctg atcagctgcg gaattccttc gtgcggaagt cgtcgtggca
     3721 ggccttgcaa gccttgccga cttccccgaa cgcggccttg aacttcgctt cgtcgccgct
     3781 cttcgattcc gcggcgagat gcgcgacggc cttctgcacg tcctcggcga gcttcttgaa
     3841 cttcggcgct tccgaccaga ccttcggatc ggccttggtc ggcgcgccct tgtcggtgcc
     3901 cgggccgaag gagttccagt gcaacccgat cagggagtcg atgagggcgg agttcttctg
     3961 cacgacggcc gcattgaagg cagccttgcc ctgcgccatc gcgcccatcg ggcccatggc
     4021 gcggccgatg acgttcatca ccgactggcg gtagcggatt tcatcctccg gtttcgggcc
     4081 ctgctgggcg aaggcgacgg cggcggaggt ggcggccagg acggctgcgg caagggcgaa
     4141 gcgtttcatc tgattccttt cgtgattggc ggttaagtgg attgcaccgt cgggcggcgt
     4201 cgcaagggtg aacagcgata gccgccgatt attccgcgag gtcatttcgc cagcttcggc
     4261 agccccgtga ccttgctgcc ggccacgacc ttgcgctcgg cgaaccagcc cttgttggtc
     4321 tcgatcgcga agcgcgcggg gccggccgcg gcgtgcgaat cgagcgtctc gggctccatg
     4381 tcggcgatat tgaggatgcg cccctcgccg tcgatgaagg cgacggagag cgggatcagc
     4441 gtgttcttca tccacatcgc gtggtatgcc ggctcttcga aaacgaagag catgccgtcg
     4501 ttcttcccga gcttcttgcg gaacatgagg cccttggccc gttgctccat cgtggccacg
     4561 acctcgacgc gcagcgcatg gccggcgacc ctgacattgc tgacgggaag cggcgcttcg
     4621 gcgagggcgg gcaacgcagc agcgaatagc gcggccgcaa ccaggcgccc tgcggtggag
     4681 cggggcagaa gggcgggtag aatggcttcc aagaagaatc tccaattgat ttggcgcacg
//
LOCUS       DASUEV010000044         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_815073, whole genome shotgun sequence.
ACCESSION   DASUEV010000044 DASUEV010000000
VERSION     DASUEV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815073"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFV55_01475"
     CDS             <1..414
                     /locus_tag="VFV55_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX4942989.1"
                     /translation="LKGTKTHENLKAAFAGESQANRRYLYFANKADVEGQNDVAALFR
                     STAEGETGHAHGHLDYLALVGDPATELPIGSSRQNLTAAVAGETHEYTDMYPGMAKSA
                     RGEGFDEIADWFETLAKAERSHANRFQKALDVLSD"
     gene            502..1842
                     /locus_tag="VFV55_01480"
     CDS             502..1842
                     /locus_tag="VFV55_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX4942990.1"
                     /translation="MSVREGNLEAPTRHPIDWKNPEYYDEAKLQEEMERVFGICHGCR
                     RCVSLCGSFPKLFDLVDEGPTGEVDGVAKERYREVVDQCYLCDVCYMTKCPYVPPHPW
                     NVDFPHLMLRAKAVKFASGKVPFRDKLLAATDALGTLATIPIVTQVTNALNRSGSARG
                     VMESALGVDRNAWVPEYATAKFRSSVARSGAFPVRDGGRTPGKVAIFSTCYINYNEPG
                     IGHDLAKILAHNEIPYVIAAKEACCGMPTLELGDLAAVAANKEKNIPALASLAREGYA
                     ILTPIPSCTLMFKQELPLLFPGDPDVLAVQEAMFDPFEYLVLRRKDGLLRTDFTTALG
                     KVSYHVPCHSRVQKIGQKTREALEMVPGTTVSTVERCSGHSGTWGCKKEFHQVAMKIG
                     RPVFRAMAEPQPDWISSDCQLGGRHIEQGMGDTLGKAQLKHPITLMRMAYGLPD"
     gene            1953..2537
                     /locus_tag="VFV55_01485"
     CDS             1953..2537
                     /locus_tag="VFV55_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX4942991.1"
                     /translation="MPKLSPGSLMTLEAYAKARNAFRAKVLAHKKPRSVHLGEHVTLL
                     FEDELTIRYQVQEMLRIEKTFEESGIQDELDAYNPLIPDGRNFKATMLIEYEDAAERG
                     VALANLRGIEDRVWLRVEGSPKVFAIADEDLERENDVKTSSVHFLRFELADEMAEALK
                     YGVALAIGVDHPNYSASVDPVNGETRAALVKDLA"
     gene            complement(2559..>3480)
                     /locus_tag="VFV55_01490"
     CDS             complement(2559..>3480)
                     /locus_tag="VFV55_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX4942992.1"
                     /translation="FPMTLTELRYVVALAQERHFGRAARKCFVTQPTLSLALAKLEDE
                     LGVRLFERNKNEVLVTPKGERIVEQARRVLDEAAKVHLLAKGSQDQLAGELRLGVIPT
                     IGPYLLPELVPILRRKAPRMPLVIEENFTGNLVPMLRDGDVDAAIIALPFTVPGVKTR
                     TLYEEPFSVVVPEGHRWAKKRRVRPDELGGENLLVLNAGHCFREQVLEACPGQSNTAG
                     LEGGRSGSSLETIRNMVASGLGVSVLPATALQPRYVNALVKEIPFTEPVPSRMVAIAW
                     RSSFSRPQAVDAVAAAILEVKLACLKKVAR"
BASE COUNT          641 a         1194 c         1099 g          546 t
ORIGIN      
        1 ctcaaaggca cgaagaccca cgaaaacctg aaagccgcct tcgcgggcga atcccaggcg
       61 aaccgccgct acctgtactt cgcaaacaag gcggacgtgg aaggccagaa cgatgtcgcg
      121 gcgctcttcc gctcgacggc ggagggcgag accgggcatg ctcatggcca cctcgactac
      181 ctcgcgctgg tgggcgaccc cgccacggaa ctgccgatcg gctcctcgcg ccagaacctc
      241 acggctgccg tcgcgggcga gacgcacgag tacaccgaca tgtaccccgg catggccaag
      301 agcgcgcgcg gcgaaggctt cgacgagatc gccgactggt tcgagaccct cgccaaggcc
      361 gagcgttccc acgccaatcg cttccagaag gccctggacg tcctgtccga ctgaagcagg
      421 acaacgcaac cccggactgc gaacaaaaag gggcgggcaa ccgccccttc ctgcatgcga
      481 tcccaccagg acccgagtcc catgagcgtg cgagaaggca acctcgaggc acccacccgc
      541 catcccatcg actggaagaa ccccgagtac tacgacgagg cgaagctgca ggaggaaatg
      601 gagcgcgtgt tcggcatctg tcacggatgc cggcgctgcg tgagcctgtg cgggtcgttc
      661 ccgaagctgt tcgacctcgt cgacgaaggt cccaccggcg aggtcgacgg cgtggcgaag
      721 gagaggtacc gggaagtcgt cgaccagtgc tatctctgtg acgtctgcta catgacgaag
      781 tgcccctacg tgccgccgca cccttggaac gtcgatttcc cgcacctcat gcttcgcgcc
      841 aaggcggtga aattcgccag cggcaaggtg cccttcaggg acaagctgct tgccgcgacc
      901 gatgcgctgg gcacgctggc gacgatccct atcgtgacgc aggtgacgaa cgcgctcaac
      961 cgatccgggt cggcgcgcgg cgtcatggaa agcgcgctcg gcgtggacag gaatgcgtgg
     1021 gtgcccgagt atgccaccgc caaattccgc tcgagcgtgg cgcgcagcgg cgcgttcccg
     1081 gtaagggatg gcggccggac gcccggcaag gtggcgatct tctccacctg ctacatcaac
     1141 tacaacgagc ccggcatcgg ccacgatctc gcgaagatcc tcgcccacaa cgagattccc
     1201 tacgtgatcg cggcgaagga ggcgtgctgc ggcatgccga cgctcgagct gggcgacctc
     1261 gcagcggtcg cggccaacaa ggagaagaac atcccggcgc tcgcgagcct ggcgcgcgag
     1321 ggctatgcga tcctcacccc gatcccctcc tgcacgctga tgttcaagca ggagcttccc
     1381 ctcctgttcc ctggcgatcc ggacgtgctg gccgtccagg aggcgatgtt cgatcccttc
     1441 gaatacctgg tgctgcggcg caaggacggg cttctcagga ccgacttcac gactgcgctg
     1501 ggcaaggtgt cctaccacgt tccctgccac tcccgggtgc agaagatcgg ccagaagacg
     1561 cgcgaagcgc tcgagatggt tccgggaacc acggtcagca cggtcgagcg ctgttccgga
     1621 cactcgggaa cgtgggggtg caagaaggaa ttccaccagg tggcgatgaa gatcggccgc
     1681 ccggtcttcc gcgccatggc ggaaccgcag cccgactgga tcagctccga ttgccagctc
     1741 ggcggccgtc acatcgagca gggcatgggc gacacgttgg gcaaggcgca gttgaagcac
     1801 cccatcaccc tgatgcgcat ggcctacggc cttcccgact gaacgtcgtc gcaccagcac
     1861 ttaccgttca tcggcgttca cctgcggttc cacgaattgc cgatcatccg tgttcatttg
     1921 tggtttcacg aattcctgaa aacacgatca ccatgcccaa actctccccc ggctccctga
     1981 tgaccctcga ggcctatgcc aaggcccgca acgccttccg tgcaaaggtg cttgcgcaca
     2041 agaagccgcg ctcggtgcac ctcggcgagc acgtgacgct ccttttcgaa gacgagctga
     2101 ccatccgcta ccaggtccag gagatgctcc gcatcgaaaa gaccttcgag gaatccggca
     2161 tccaggacga gctcgacgcc tacaaccccc tcattcccga cgggcgcaat ttcaaggcca
     2221 cgatgctgat cgagtacgag gatgccgcgg agcgcggcgt ggccctggcg aatctgcggg
     2281 gcatcgagga ccgcgtgtgg ctccgggtcg agggctcgcc gaaagtcttc gcaattgccg
     2341 acgaggacct cgagcgcgag aacgacgtga agacatcctc ggtgcacttc ctgcgcttcg
     2401 agctcgccga cgagatggcc gaggcgctca agtacggcgt ggcgctcgcc atcggcgtgg
     2461 atcacccgaa ctactcggcg tccgtcgacc cggtgaatgg cgagacccgc gcggcgctcg
     2521 tcaaggacct cgcctagagg cccggcaggt ccgccgttct acctcgcgac cttcttcagg
     2581 caggcgagct tcacttccag gatcgccgcc gccaccgcgt ccacggcctg cggacgcgaa
     2641 aagctcgagc gccacgcgat ggcgaccatg cgcgacggca cgggttcggt gaacgggatt
     2701 tccttcacga gagcgttcac gtagcgcggc tgcagcgccg tggccggcag cacgctgacg
     2761 ccgagccccg aggccaccat gttgcggatg gtctccagcg agcttcccga ccgtcccccc
     2821 tcgaggccgg cggtgttgga ctggccgggg cacgcctcga ggacctgctc gcgaaagcag
     2881 tggccggcat tcagcaccag caggttctcg ccgcccagct cgtcgggacg cacgcgcctc
     2941 ttcttagccc accggtgccc ctcgggcacc acgacgctga acggctcctc gtagagcgtc
     3001 ctcgtcttca ccccgggaac cgtgaacgga agcgcgatga tggccgcgtc gacatcgcca
     3061 tcgcgcagca tcgggacgag attgccggtg aagttctcct cgatcaccag cggcatccgc
     3121 ggcgccttcc tgcgcaggat cggcaccagt tccggcagca ggtacggccc gatcgtcggg
     3181 atcacgccga gccgcaattc cccggccagc tggtcttggc tgcccttggc gagcaaatgg
     3241 accttcgcgg cctcgtccag cacgcgccgc gcctgttcca cgatccgctc gcccttcggc
     3301 gtgacgagca cctcgttctt gttgcgctcg aagaggcgca cgccgagctc gtcctcgagc
     3361 ttggcgagcg cgagcgagag cgtgggctgg gtgacgaagc acttccgggc ggcgcgcccg
     3421 aagtggcgct cttgcgccag ggccaccacg taacgcagtt cggtgagggt catcgggaag
//
LOCUS       DASUEV010000045        13701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1502538, whole genome shotgun sequence.
ACCESSION   DASUEV010000045 DASUEV010000000
VERSION     DASUEV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502538"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1841)
                     /locus_tag="VFV55_01495"
     CDS             complement(57..1841)
                     /locus_tag="VFV55_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX4942993.1"
                     /translation="MSDLHLGLIGNSSIGALVDGAGEFVWACLPRPDGDAVFCSLLRG
                     HRGEADFGFMGVELSWLARTEQEYLPNTPILVTRLFDDRGGAVEIVDFAPRFRQFGRL
                     FCPAQFVRIVRPVSGSPRIRVRVRPADDYGRRRPDVTSGSNHVRFVGSANVLRLTTDC
                     SITAILEEMPFVLRDGIAFVLGQDETMQGSVAEVARRFLELTSDYWHDWVRDLSIPFE
                     WQDEIIRAAITLKLAAYDDTGAVIAAITTSIPESPNSGRNWDYRFCWLRDAYFVVNAL
                     NRLNATRTMERYLGYIINVAAGSDGHLQPVYRITGRADLDEHVVDTLPGYRGMGPVRI
                     GNQAYRQVQNDVYGSTILAATHAFFDRRLAAVGHAGLFRRLEILGERAAALHASPDAG
                     LWELRGTAHVHTFSSVMCWVACDRLAKIARRLGITEAVRRWQEQADVIHASICERAWS
                     AKRASFVATFEGDALDASLLLLVELGFLEAGDPRFAGTVAAIERELKRGDFIFRYTQP
                     DDFGAPENAFLVCTFWYIDALAALGRREEARALFEATLARRNRLGLLSEDIDPATGEL
                     WGNFPQTYSMVGLINSATRLSIPWDQAF"
     gene            complement(1838..2584)
                     /gene="otsB"
                     /locus_tag="VFV55_01500"
     CDS             complement(1838..2584)
                     /gene="otsB"
                     /locus_tag="VFV55_01500"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX4942994.1"
                     /translation="MPAHAAMFLDIDGTLVHYADRPDAVHIDHALCALLERLRDVAGG
                     ALALISGRSIADIDALFAPLRFAAAGQHGAERRSADGAMHYHLPLAPRLNAQAAILRR
                     LALEHPGLLLEEKGVSLALHYRGDPSAAALVEREARRVADALGDDFELQSGKHVCEVK
                     PSGKNKGTAIAEFMAEVPFTGRPPVFVGDDLTDELGFELVNRIGGHSVKVGPGPTRAH
                     WRLGNADEVRLWLSDFAAPGRAQPAGRAAS"
     gene            complement(2623..4878)
                     /locus_tag="VFV55_01505"
     CDS             complement(2623..4878)
                     /locus_tag="VFV55_01505"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX4942995.1"
                     /translation="MRLSLRFIIPLILALGAIAYYIAPLVDDLTLKWFVRDLDIRTRL
                     IATAIQEPLADAMTADVGPKARQERLNAVFNRLIQDERLFAIGYCDNTGKFAYRTVTL
                     PKDITCRPPNSPPEEAGRVHHFAGGSLHVATNIVTIGGARMGELVIAHDMSFIERRSA
                     DTKKYIVYLFAGIGAVIALITVVIAELSWRGWVAGMKALIRGQLLAESPEPRSKELKP
                     LARDLEALVRDLETERRMRDESQMSWVPETLRKILRDDLKGDEILIVSNREPYIHVRE
                     GERVRVQRPASGLVTALEPVMRACSGTWIAHGAGNADREVVDANDHVMVPPENPSYRI
                     RRVWLTKEEEAGYYYGFANEGLWPLCHIAHTRPVFRASDWEYYRAVNRRFAETVVSES
                     RTPNPIVLVQDYHFALLPRMIRERLPQATIITFWHIPWPNPEAFGICPWRSEVLEGLL
                     GSSILGFHTQFHCNNFFDTVDRYLEARVDRETFTISYGGEPTEVHRYPISIEWPLKGL
                     AEQPPVAECRKAIREQLGLAPDVRLGVGVDRLDYTKGILERFAAIERFLEMEPAWIGK
                     LSFVQIAAPSRSSIDEYQLLDARVRAAAQRINDRFGTPGCQPIVLKVEHHDAGRIYTH
                     YRAADFCFVSSLHDGMNLVAKEFVATREDERGVLLLSQFAGAARELPEALIVNPYDTE
                     QCAAAVREALTMSPDEQRARMRSMRALLLEFNVYRWAGRMLLDAARMRNRRRLAAGAR
                     VTAVAAGARRT"
     gene            complement(5016..5900)
                     /locus_tag="VFV55_01510"
     CDS             complement(5016..5900)
                     /locus_tag="VFV55_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4942996.1"
                     /translation="MNPRSTPLSAMAAMCAIWGYSWIVMKIALRHAHPFDFAAERLVM
                     GAVLLFAISAATGRRLTLSSYRMVAVLGFVQVGLFVILTHFALLAAGPGKTSVLTYTM
                     PFWMIVFARLLLHERMRGAQWLSVAVAFAGLVLIVSPWRLASLEGSLLAVASGAVWAL
                     AAVMSKRWPTNEAEPLTFTAWQLAFGSIPLVVLSLLHPHEAPRWNGEYALALAYSTVF
                     ATAGGWWLWTYVLSHAPAGVTGLNTLAIPVIAVIASWLQLGERPPPHELLGMALIGVA
                     LGLLAWLGVRRAEATPVD"
     gene            5941..6375
                     /locus_tag="VFV55_01515"
     CDS             5941..6375
                     /locus_tag="VFV55_01515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4942997.1"
                     /translation="MTNSPQFRVRLVGWREASATLTRIRTTVFVVEQNVPAGLEMDGR
                     DGDCAHALAETADGEPIGTGRLMPDGRIGRMAVLASWRGRGVGAAILDALMAEAKRRG
                     FRETYLHAQSHAQAFYARHGYVVEGDEYLEAGIPHIGMRATL"
     gene            7123..8301
                     /locus_tag="VFV55_01520"
     CDS             7123..8301
                     /locus_tag="VFV55_01520"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX4942998.1"
                     /translation="MFHGLLNLPWWGDVLALLVLTQITIAGVTLYLHRCEAHRSLDLH
                     PIVSHFFRFWMWLTTGMITKEWAAIHRKHHAKCETAEDPHSPQVYGINRVLFGGVMLY
                     VQESHHPETMQRYGVGTPHDWIERRIYTPHHKKGILLLALVNIVLFGAVPGMLMFGVQ
                     MAWIPFWAAGVINGIGHYWGYRNFQTEDASCNIIPVAAWIGGEELHNNHHAFPTSAKF
                     SVRWYEFDLGWLYIRALSALRLARAKKLPPKVKLDPSKLEVDAQTLQAIAINRYEVLA
                     RYAKSMKLAWNEEAARLREQKILADRAEARRLRQCITRGTVQSQAEREAVARALESSP
                     RLATWYKMREELARTWERSTLSTEQLVQQLRDWCDRAEASGIEALAEFSQRLRRYAAA
                     "
     gene            8317..9783
                     /locus_tag="VFV55_01525"
     CDS             8317..9783
                     /locus_tag="VFV55_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX4942999.1"
                     /translation="MPDPPPGAALVFGASGYIGSHLVPALAARGWRVRAAARNLKVLE
                     ARGWEGVEPAAADALRPETLPAVLAGIDVAYYLVHSMSAGADFGRLDLAAADNFARAC
                     AAAGVKRIVYLGGLVPVGAVSEHLLSRRDTGERLRSHPVPVTEIRAGIIVGPGSAAFE
                     VMRDLVLHLPVMVTPRWVRAKSPPIALDNLLHYLVEAPRIAAMAGGIYDAAGPETLTY
                     EAMMRAICRILGRRAPWILPVPVLTPELSAYWLRFVTAVPTNIARALIGGLKHDFTAD
                     DAAIRTLIPQRLLDFDESVRAALQAEARHAVAARWTEGAFMFRDYRPDFAYYAKRAGG
                     EARTPARARSLWRAVAAIGGDNRYYAYNILWTLREIADWLVGGVALHRGRRDPDHVRV
                     GDVIDSWRVIGVEPERRLTLVFGMKAPGAGVLEIEIEEISPDERRIAVTAYWHPSGFL
                     GLAYWRAMFPAHLVLFRALAKEIARRAMVSDTVSDTGA"
     gene            complement(9858..10013)
                     /gene="rpmG"
                     /locus_tag="VFV55_01530"
     CDS             complement(9858..10013)
                     /gene="rpmG"
                     /locus_tag="VFV55_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX4943000.1"
                     /translation="MREKIKLESTAGTGHFYTTTKNKKTKPEKLEFMKFDPKARKHVA
                     YKETKLK"
     gene            complement(10025..10261)
                     /gene="rpmB"
                     /locus_tag="VFV55_01535"
     CDS             complement(10025..10261)
                     /gene="rpmB"
                     /locus_tag="VFV55_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX4943001.1"
                     /translation="MSRVCQVTGKGPIVGNHVSHANNKTKRRYLPNLQYRRLWVESEN
                     RYVKLRLTTAGLRTIDKKGIDVVLAEMRARGEAI"
     gene            complement(10364..11038)
                     /gene="radC"
                     /locus_tag="VFV55_01540"
     CDS             complement(10364..11038)
                     /gene="radC"
                     /locus_tag="VFV55_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX4943002.1"
                     /translation="MALADWPAAGRPRERLAAQGPAALSDAELVALMLRTGVAGRPAV
                     EIAGELLDRFGRVSRLLAAPVPEVAAVRGVGAARAAGFAAAMELARRSLSEDMQARDS
                     LASPMAVRGYLRLRMQHLGHEVFWVVFLDAQNRVIAAEELFRGTLTQTSVYPREVVKQ
                     ALAHNAAGVILAHNHPSGVAEPSIQDQALTRSLAESLALVDVKVLDHFIVAPGACLSF
                     AERGLL"
     gene            11069..12298
                     /gene="coaBC"
                     /locus_tag="VFV55_01545"
     CDS             11069..12298
                     /gene="coaBC"
                     /locus_tag="VFV55_01545"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX4943003.1"
                     /translation="MEETGKRRVLLGVTGGIAAYKVAELARALIRNNVDVQVAMTEAG
                     TKFVTPATFQALTGRPVVTDLWDASFPNAMPHIELSRGADAIVIAPASADFLAKLAHG
                     LADDLLSTTCLARNCPLLVAPAMNREMWDNAATQRNVSLLRADGVVILGPAAGDQACG
                     EVGMGRLPEPEELLQSILDFLSPRRLAGRKVLITAGPTFEAIDTVRGITNQSSGKMGF
                     ALAEAAAGLGADVTLVSGPTALPPPANAHFVYVTSAAEMLNAVNAHASSADLFFSVAA
                     VADYTPEAPATRKIKKSGEPLEIRLKPTVDILAQVAKMPGAPFCVGFAAESENLAEYA
                     QAKRKRKGIPMIVANLVQHTIGRDENEVTIYDDAGAHPIARAPKSRIAHAVLEHAIAL
                     FEAKHGGATVTPIKHVS"
     gene            12572..13021
                     /gene="dut"
                     /locus_tag="VFV55_01550"
     CDS             12572..13021
                     /gene="dut"
                     /locus_tag="VFV55_01550"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX4943004.1"
                     /translation="MKKLDVKILDERMRSQLPAYATSGSAGLDLRACVDEPLTLHPGD
                     SALVPTGLAIHVADAGHAAVIIPRSGLGHRHGIVLGNLVGLIDSDYQGQLLVSCWNRG
                     RETFVVNPMERIAQLVVIPVVQVELNVVESFDESSRGEGGFGSTGKH"
     gene            13030..13395
                     /locus_tag="VFV55_01555"
     CDS             13030..13395
                     /locus_tag="VFV55_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX4943005.1"
                     /translation="MHGIVLFAHGARDPEWARPFEAIRDRVRARRPEFPVELAFLDFM
                     TPHLDEAVHGLAAKGVAAITIFPLFMAPGGHIRNDLPRIVEDLRKAHPGVPIAIQTAV
                     GEVPEILEAIAGWVLAGVD"
     gene            complement(13397..>13701)
                     /locus_tag="VFV55_01560"
     CDS             complement(13397..>13701)
                     /locus_tag="VFV55_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943006.1"
                     /translation="RQPMRRPAILFFPFLLASCAVIQPSPPKEETPEQAKERRTKAPR
                     PTYNLTGYPPPVRDGYIDGCESAKKSEFGRKDAKRFAADDQYRMGWNDGFSICSRR"
BASE COUNT         2212 a         4648 c         4715 g         2126 t
ORIGIN      
        1 aatcctggcg agcgtgaagc actacaaggc gcccaagcgc aagggctgac ccctggtcag
       61 aacgcctggt cccaggggat gctcagccgc gtcgcgctgt tgatcaggcc caccatgctg
      121 taggtctgcg ggaagttgcc ccacaactcg cccgtggcgg ggtcgatgtc ctccgacagc
      181 aggcccaggc ggttgcgccg cgcgagcgtg gcctcgaaga gcgcacgcgc ttcctcccgc
      241 cgtccgaggg cggccagcgc gtcgatgtac cagaaggtgc agacgaggaa cgcgttctcg
      301 ggcgcgccga agtcgtcggg ctgcgtgtag cggaagatga agtcgccgcg cttgagctcg
      361 cgctcgatgg cggcgacggt gcccgcgaag cgcgggtcgc cggcctcgag gaagcccagt
      421 tccacgagca ggagcaggct cgcgtcgagc gcgtcgccct cgaaggtggc cacgaaactc
      481 gcgcgctttg ccgaccacgc gcgctcgcag atcgatgcgt ggatgacgtc ggcctgctcc
      541 tgccatcgcc gcacggcctc cgtgatcccg aggcgccggg cgatcttggc cagccggtcg
      601 caggccaccc agcacatcac gctcgagaac gtgtgcacat gcgccgtgcc gcgcagctcc
      661 cagaggccgg cgtcggggct cgcgtgcagc gctgccgccc gttcacccag gatctccagc
      721 cggcggaaga gccccgcatg gcccacggcc gccaggcgcc ggtcgaagaa ggcgtgcgtg
      781 gccgcgagga tcgtcgagcc gtacacgtcg ttctgcacct gccggtatgc ctggttgccg
      841 atgcgcaccg gacccatccc gcgatagccg ggcagcgtat cgacgacgtg ctcgtccaga
      901 tcggcccggc cggtgatgcg gtacaccggc tgcaggtggc catcggatcc cgcagccacg
      961 ttgatgatgt aaccgaggta gcgctccatc gtccgcgttg cgttgaggcg attgagcgcg
     1021 ttcacgacga aataggcgtc gcgcagccag cagaagcggt aatcccagtt gcgtccgcta
     1081 ttcggggatt ccgggatcga ggtggtgatc gccgcgatca ccgcgccggt gtcgtcgtag
     1141 gcggcgagct tcagcgtgat ggccgcgcgg atgatctcgt cctgccattc gaacgggatg
     1201 gagaggtcgc gcacccagtc gtgccagtag tccgaggtga gctcgaggaa gcgccgcgca
     1261 acctcggcca ccgagccctg catcgtctcg tcctgcccga gcacgaaggc gatgccgtcg
     1321 cgcaggacga aaggcatttc ctcgaggatc gcggtgatcg agcagtcggt ggtcaggcgc
     1381 agcacgttcg cggaaccgac gaaccggacg tgattggagc cggacgtcac gtccgggcgc
     1441 cgcctgccgt agtcgtcggc gggccgcacg cgcacgcgga tgcgcgggct tcccgagacg
     1501 gggcgcacga tgcgcacgaa ctgggcgggg cagaagaggc gcccgaactg gcggaaacgc
     1561 ggcgcgaaat cgacgatctc gaccgcgccg ccgcggtcgt cgaagagccg cgtcacgagg
     1621 atgggcgtgt tcggcaggta ctcctgctcg gtgcgcgcga gccagctcaa ctccacgccc
     1681 atgaaaccga agtcggcctc gccgcgatgg ccgcgcagca gcgagcagaa caccgcatcg
     1741 ccgtcgggcc gcggcaggca ggcccacacg aactctcccg cgccatcgac cagcgcaccg
     1801 atgctcgagt tgccgatgag cccaaggtgc aggtcgctca tgacgccgcc cgccccgcgg
     1861 gttgcgcgcg gccgggcgcc gcgaagtccg aaagccagag ccgcacttcg tcggcgttgc
     1921 cgagccgcca gtgggcgcgg gtcggccccg gccccacctt caccgagtgg cctccgatcc
     1981 ggttcacaag ctcgaagccc aactcgtcgg tgaggtcgtc gccgacgaag acgggcgggc
     2041 gcccggtgaa cgggacttcc gccatgaatt cggcgatggc cgtgcccttg ttcttgccgc
     2101 tgggcttcac ttcgcagacg tgcttgccgg actgcaattc gaagtcgtca cccagcgcgt
     2161 cggccacgcg acgtgcctcg cgctccacga gcgccgccgc ggacggatcg ccgcggtagt
     2221 gcagcgcgag gctcacgccc ttctcctcga ggagcagccc cgggtgctcg agcgcgaggc
     2281 gccgcaggat tgcggcctgg gcgttgagcc gcggggcgag aggcaggtgg taatgcatcg
     2341 cgccgtccgc ggaccggcgt tcggcgccgt gctggcccgc cgcggcgaag cgcaacggcg
     2401 cgaagagcgc atcgatgtcg gcgatcgacc ggccgctgat cagggcgagg gccccgcctg
     2461 cgacatcccg gagccgttcg aggagcgcac agagggcatg gtcgatgtgc acggcgtcgg
     2521 ggcggtcggc gtagtggacg agcgtaccgt cgatgtcgag gaacatggcc gcgtgcgccg
     2581 gcatgggggg agcgccggtc gtcgtcggaa atcccgcgtc cgtcatgtgc gccgggcccc
     2641 cgcggcgacg gcggtaacgc gcgcgccggc cgccagccgg cggcggttgc gcatgcgcgc
     2701 cgcgtcgagc agcatccgcc ccgcccagcg gtacacgttg aactccagca ggagcgcccg
     2761 catgctgcgc atccgcgcgc gctgctcgtc gggcgacatg gtcaatgcct cgcgcacggc
     2821 cgcggcacac tgctcggtgt cgtaagggtt gacgatgagc gcctcgggca gctcccgcgc
     2881 ggctccggcg aactgcgaca acagcaggac gccgcgctcg tcctcgcgcg tggccacgaa
     2941 ctccttggcc acgaggttca tgccgtcgtg caggctggag acgaagcaga agtcggccgc
     3001 gcggtaatgg gtgtagatgc gcccggcatc gtgatgctcg accttgagaa cgatgggctg
     3061 gcacccgggc gtgccgaagc ggtcgttgat gcgctgggct gccgcgcgca cgcgcgcgtc
     3121 cagcaactgg tactcgtcga tgctcgagcg gctgggcgcg gcgatctgca cgaacgaaag
     3181 cttgccgatc caggcgggtt ccatctcgag gaagcgctcg atggcggcga agcgctcgag
     3241 gatgcccttc gtgtagtcga gtcgatcgac gcccacgccc aggcgcacgt cgggcgcgag
     3301 gcccagctgc tcgcggatcg ccttgcggca ctccgccacg ggcggctgct cggcgaggcc
     3361 cttcagcggc cactcgatcg agatcgggta gcggtgcacc tccgtgggct cgccgccgta
     3421 ggagatggtg aaggtctcgc ggtcgacgcg cgcctccagg tagcggtcca ccgtgtcgaa
     3481 gaaattgttg cagtggaact gcgtgtggaa acccaggatc gaggatccga gcaggccttc
     3541 gaggacctcc gagcgccatg ggcagatgcc gaaggcctcc ggattgggcc acgggatgtg
     3601 ccagaaggtg atgatcgtgg cttgcggcag ccgctcgcgg atcatgcgcg gcagcagggc
     3661 gaagtggtag tcctgcacga ggacgatcgg gttgggcgtg cgcgactccg agacgacggt
     3721 ctcggcgaag cgccggttca cggcgcggta gtattcccag tcggaggcgc ggaacacggg
     3781 gcgagtgtgc gcgatgtggc acagcggcca caggccctcg ttggcgaagc cgtagtagta
     3841 gccggcctct tcctccttcg tgagccagac ccggcggatg cggtaggagg ggttctcggg
     3901 cggcaccatc acgtggtcgt tggcgtcgac cacctcgcgg tcggcgttgc ccgcgccatg
     3961 ggcgatccag gtgccggagc aggcgcgcat cacgggctcg agcgccgtga cgaggccgct
     4021 cgccgggcgt tgcacccgca cgcgctcgcc ctcccgcacg tggatgtagg gctcgcggtt
     4081 ggacacgatg aggatctcgt cgcccttcag gtcgtcgcgc aggatcttgc gcagcgtctc
     4141 cggaacccag ctcatctgcg actcgtcgcg catgcgccgc tcggtctcga ggtcgcgcac
     4201 cagcgcctcg aggtcgcggg cgaggggctt cagctccttc gagcggggct cgggggattc
     4261 cgcgaggagc tgcccgcgga tgagcgcctt catgccggcg acccagccgc gccacgacag
     4321 ctcggcgatc accacggtga tgagggcgat caccgcgccg atgccggcga agaggtagac
     4381 gatgtacttc ttcgtgtcgg cgctgcgccg ctcgatgaag ctcatgtcgt gggcgatgac
     4441 cagctcgccc atgcgcgcgc cgccgatcgt cacgatgttg gtcgccacgt gcagcgagcc
     4501 gccggcgaag tgatggacgc ggccggcttc ctcgggcggg gagttcggcg gccggcaggt
     4561 gatgtccttg ggcagcgtca ccgtccggta cgcgaacttc cccgtgttgt cgcagtagcc
     4621 gatcgcgaag agccgctcgt cctggattag gcggttgaag accgcattga ggcgctcctg
     4681 gcgcgccttg ggcccgacat cggcggtcat cgcgtcggcg agcggttcct ggatcgccgt
     4741 ggcgatgagc ctggtgcgga tgtcgaggtc gcgcacgaac cacttgaggg tgagatcgtc
     4801 gacgagcggc gcgatgtagt acgcgatcgc gccgagcgcg aggatgagcg ggatgatgaa
     4861 gcgaagcgag agccgcattg ccccggttcc ggctggtgcg cctcgcgaga aagcttctga
     4921 cggctgcgat ggcgcattgc gggcccccgc gcgatcgccg ggggacgaac cgccatctta
     4981 tctcctcgaa gccggctcgg ggtgacgcgg cctgttcaat ccaccggcgt ggcctcggcg
     5041 cggcgaacgc ccagccacgc gaggagcccc agcgccacgc cgatgagcgc catgccgagg
     5101 agctcgtgcg gcggcggtcg ttcgccgagc tgcagccagg acgcgatgac ggcaatcacg
     5161 gggatggcca gcgtgttgag cccggtgacg ccggcgggcg cgtgggaaag cacgtaggtc
     5221 cacagccacc agccgcccgc ggtcgcgaag accgtggaat aggcgagcgc gagcgcgtac
     5281 tcgccgttcc agcgcggcgc ctcatgcggg tgcaggagcg agaggaccac gagcggtatc
     5341 gagccgaacg cgagttgcca cgccgtgaag gtgagcggct ccgcttcatt cgtgggccag
     5401 cgcttcgaca tcacggcggc cagtgcccac acggcgcccg aggcgacggc gagcaggctg
     5461 ccctcgaggc tcgcgagcct ccacggcgac acgatcagca cgaggcccgc gaacgcgacg
     5521 gcgacggaga gccactgcgc gccacgcatg cgctcgtgaa ggagcaggcg ggcgaacacg
     5581 atcatccaga aaggcatcgt ataggtgagg accgaggtct tgcccggtcc cgcggcgaga
     5641 agcgcgaaat gggtgaggat cacgaagagc ccgacctgga cgaagcccag caccgccacc
     5701 atcctgtagc tcgaaagcgt gagcctgcgc ccggtggcgg cgctgatcgc gaagagaagg
     5761 acggcgccca tcaccagccg ctcggccgcg aaatcgaagg gatgcgcgtg gcgcagcgcg
     5821 atcttcatca cgatccacga atagccccag atcgcgcaca tggcggccat ggcggagagc
     5881 ggggtggaac gggggttcat ggcggcggcg ggtagaattg cggcgagccg cgactctacc
     5941 atgacgaatt cccctcaatt ccgcgtgcgc ctggtcggct ggcgcgaggc atcggccacg
     6001 ctcacccgca tccgcacgac ggtcttcgtc gtcgagcaga acgtgcccgc gggcctcgag
     6061 atggacgggc gcgacggcga ctgcgcgcac gcgctggccg agaccgcgga cggcgaaccc
     6121 atcggcaccg gccgcctcat gcccgacggg cgcatcggcc gcatggccgt gctggcgtcc
     6181 tggcgcggca ggggcgtcgg tgcagcgatc ctcgacgcgc tgatggccga ggcgaagcgg
     6241 cgcggctttc gcgagacgta cctgcacgcg cagtcgcacg cgcaggcctt ctacgcccgg
     6301 cacggctacg tcgttgaggg cgacgagtac ctcgaggcgg gcatcccgca catcggcatg
     6361 agggctacgc tgtaaacacg agcgggtcga ggcggtggca gtggaagtcg atcgcaatcc
     6421 tgggcacgca agcgtgaccg tcggatgggt cccaaaaatc cgaggccagg caaaacaagc
     6481 caggcctcgg atttttaccc atctaataac gagggcgctg cgctgcctcg tccgcgacgg
     6541 tcgcgtgcca aggattgcga tcgacttcca ccgccaccgc caccgcctcc cacgctccgt
     6601 ggatgcagca ccgagtgagg ccacatcgga ccgcgccctt atctgctgtc gaatgcgcca
     6661 cgctgcctct tcatgtgggc cggggatggg gcaagtctat cggcgtcctt gggcacgcga
     6721 ccgtcgcgat gggagagcgc agcggcccca tctctcgatg ggtaaaaagt cggggacaga
     6781 cgtgtttggc tggccccgac tttttgggac ccatccgacg gtctcgcttg cgtgcccagg
     6841 acgccgatag acttgcccca tcgccggcct aacacctaat tcgccgttca cgtttgcgtg
     6901 cccaggacgc cgatagactt gccccatccc cggccctcat cctaattcgc cgcgtgcatc
     6961 agtcaccggg ttcatgtccc tcgatgctgg aggaaatccg cccggaagcc ggcggagagc
     7021 cgaatttcgc tcgcgcccgg gttcccgggc cggcaaaaat gctaccctag gtatgcttcg
     7081 aatccgcaac cagtcgggac tcccgaaagg cgtcgttaca tgctgtttca tggtctcctg
     7141 aaccttccct ggtggggcga cgtgctcgcg ctactcgtcc tcacccagat cacgatcgcg
     7201 ggcgtgacgc tttacctgca tcgctgcgag gcgcaccggt cgctcgacct gcacccgatc
     7261 gtgagccatt tcttccgctt ctggatgtgg ctcaccacgg gcatgatcac caaggagtgg
     7321 gcggcgatcc accgcaagca ccacgccaag tgcgagaccg ccgaggatcc gcacagcccg
     7381 caggtttacg gcatcaatcg cgtcctcttc ggcggcgtga tgctctacgt gcaggagtcg
     7441 caccaccccg agacgatgca acgctacggc gtcggcacgc cgcacgactg gatcgaacga
     7501 aggatctaca cgccgcacca caagaagggc atcctgctgc tcgccctggt gaacatcgtg
     7561 ctcttcggcg cggtgcccgg catgctgatg ttcggcgtcc agatggcctg gattccgttc
     7621 tgggccgccg gcgtcatcaa cggcatcggc cactactggg gctaccgcaa cttccagacc
     7681 gaggacgcca gctgcaacat cattcccgtc gcggcatgga tcggcggcga ggagttgcac
     7741 aacaaccacc acgccttccc gacctcggcc aagttttcgg tgcgctggta cgagttcgac
     7801 ctggggtggc tctacatcag ggccctgtcg gccctgcgcc ttgcccgcgc gaagaagctg
     7861 ccgcccaagg tgaagctcga cccctcgaag ctcgaggtgg acgcgcagac gctgcaggcc
     7921 atcgcgatca accgctacga agtgctggcc aggtacgcga agtcgatgaa gctcgcctgg
     7981 aacgaggaag cggcgcggtt gcgggagcag aagattctcg ccgaccgggc cgaggcaagg
     8041 cgccttcgcc agtgcatcac gcgcggcacc gtgcagtcgc aggcggagcg cgaagccgtc
     8101 gcgcgcgccc tcgaatccag cccgcggctg gccacgtggt acaagatgcg cgaggagctc
     8161 gccaggacgt gggaacgctc cacgctttcc accgagcagc tcgtgcagca actgcgcgac
     8221 tggtgcgacc gcgccgaggc gagcggcatc gaggccctcg cggaattctc gcagcgcctg
     8281 cggcgctacg ccgcggccta gcgccccatc gctcgcctgc ccgaccctcc gccgggcgcc
     8341 gcgctcgtct tcggggcgag cggctacatc ggaagccatc tcgtccccgc gctcgccgcg
     8401 cggggatggc gcgtgcgcgc cgcggcgcgc aatctcaagg tcctcgaggc ccgcggctgg
     8461 gagggcgtcg agccggccgc agccgacgcg ctgaggccgg agacgcttcc ggccgtcctc
     8521 gcgggcatcg acgtcgcgta ctacctggtt cactcgatgt cggcgggagc cgacttcggc
     8581 cggctcgatc tcgcggccgc ggacaacttt gcgcgggcct gcgccgccgc cggcgtgaag
     8641 cgaatcgtct acctcggcgg gctcgtgccc gtcggcgcgg tctcggagca ccttctgtcg
     8701 cggcgtgaca ccggcgagcg cctgcgctcg caccccgtgc ccgtcaccga aatacgcgcg
     8761 gggatcatcg tcgggcccgg ctccgcggca ttcgaagtga tgcgcgacct cgtgctccat
     8821 ctgcccgtca tggtgacgcc gcgctgggtg cgcgcgaaat cgccacccat cgcgctcgac
     8881 aacctgctgc actacctcgt cgaggcgccg cgcatcgcgg ccatggcggg cggcatctac
     8941 gatgctgccg gcccggaaac gctcacctac gaggcgatga tgcgcgccat ttgccgcatc
     9001 ctggggcggc gcgcgccgtg gatcctgccg gtgccggtcc tcacgcccga actttccgcg
     9061 tactggctca ggttcgtgac cgcggtgcca accaacatcg cccgcgcgct catcggcggg
     9121 ctcaagcacg acttcacggc ggacgatgcg gcgatccgca cgctcatccc gcagcgcctg
     9181 ctcgacttcg atgaatcggt gcgcgccgcg ctgcaggccg aggcacgcca cgcggttgcc
     9241 gcgcgctgga ccgaaggcgc cttcatgttc cgcgactacc gccccgactt cgcgtactac
     9301 gccaagcgcg cgggcggcga ggcgcgcacg ccggcgcgtg cccggtccct gtggcgcgcc
     9361 gtcgcggcca tcgggggcga caaccggtac tacgcgtaca acatcctgtg gacgcttcgc
     9421 gagatcgccg actggctggt cggtggcgtc gcgctccacc gcggccggcg cgatcccgac
     9481 cacgtccgcg tcggcgacgt gatcgattcc tggcgcgtga tcggcgtcga gcccgagcgg
     9541 cgcctcacgc tggtgttcgg catgaaggcc cccggcgcgg gcgtcctcga gatcgagatc
     9601 gaggaaatct cgccggacga gcggcgcatc gccgtgacgg cctattggca cccctccggt
     9661 ttcctggggc tcgcctattg gcgcgccatg ttcccggcgc atctcgtgct ctttcgcgcg
     9721 ctcgcgaagg aaatcgcgcg aagggcgatg gtgtcggaca cggtgtcaga caccggtgcc
     9781 tgacgccgtg tccgataccg caatgaaaaa ccccgccgtg gcggggtctt tcggggtgcg
     9841 gtgcgggagc cgcgatgtta cttgagcttc gtttccttgt aggccacgtg cttgcgggcc
     9901 ttgggatcga atttcatgaa ctcgagcttc tccggcttcg tcttcttgtt cttggtggtc
     9961 gtgtagaagt ggcccgtgcc ggcggttgat tcgagcttga tcttttcgcg catggtcggt
    10021 tcccctagat cgcttcgccg cgggcgcgca tttcagccag caccacgtcg atgcccttct
    10081 tgtcgatggt gcgaaggccc gcggtggtga ggcgcagctt cacgtagcgg ttctcgcttt
    10141 cgacccagag cctgcggtac tgcaggttgg gcaggtagcg gcgcttcgtc ttgttgttgg
    10201 cgtgggacac gtggttgccc acgatgggcc ccttgccggt gacctggcag actcgagaca
    10261 tgggatttct ccgatactgc gccccgaaaa gccgaggcgg aaagccagtg attatagccc
    10321 agggctgccc gtgccttcaa gctgatttca ttggcctttc ccgctagagc agtccgcgct
    10381 cggcaaacga caggcaggcc cccggggcga cgatgaagtg gtccagcacc ttcacgtcca
    10441 ccagcgccag ggattccgcc agcgagcgcg taagtgcttg atcctgaatg gagggctccg
    10501 ccacgccgga ggggtggttg tgggccagga tgacgccggc cgcgttgtgc gccagcgcct
    10561 gcttgaccac ctcccgggga tacacgctgg tctgcgtgag cgtgccgcgg aagagctcct
    10621 cggcggcgat gacccggttc tgggcatcga ggaacaccac ccagaagact tcgtgcccca
    10681 ggtgctgcat gcgcaggcga aggtagccgc gcaccgccat cggcgacgcg aggctgtcgc
    10741 gggcctgcat gtcctcggaa agcgagcggc gggcgagttc catggccgcc gcgaaaccgg
    10801 ccgcccgggc cgcccccacg ccgcgaacgg cagccacttc cggcaccggc gcggcgagca
    10861 gccgcgacac gcgaccgaag cggtccagca actcgccggc gatttccacg gcgggccggc
    10921 ccgccacccc cgtgcgcagc atgagggcga cgagttcggc atcggaaagg gcggcgggcc
    10981 cttgtgcggc aagacgttcg cggggccgcc cggcggcggg ccaatcggca agggccatgg
    11041 gtcgtgactc tcctagtaga atgatccgat ggaagaaacg ggaaagcgcc gcgttctact
    11101 gggggtcacc gggggcatag ccgcgtacaa ggtggcggaa ctcgcgcggg cgctcatccg
    11161 caataacgtc gacgtgcagg tcgcgatgac agaggccggc acgaaattcg tgacgcctgc
    11221 gaccttccag gcgcttaccg gaaggccggt cgtcaccgac ctgtgggacg cgagcttccc
    11281 caacgccatg ccgcacatcg agctttcgcg cggcgcggat gcgatcgtga tcgcgccggc
    11341 cagcgcggac ttcctcgcca agctcgcgca cgggcttgcc gacgacctgc tctcgaccac
    11401 ctgcctcgcg cgcaattgcc cgctcctggt ggcccccgcg atgaatcgcg agatgtggga
    11461 caacgcggcc acgcaacgca acgtgagcct gctgcgcgcc gatggcgtgg tcatcctcgg
    11521 ccccgcggcc ggcgaccagg cctgcgggga agtcggcatg gggcgcctcc ccgagcccga
    11581 ggagctgctg cagtccatcc tcgatttcct ctctcccagg cgcctcgccg ggcgcaaggt
    11641 gctcatcacc gccggcccca cgttcgaagc catcgacacg gttcggggca tcaccaacca
    11701 gagctcggga aagatgggct ttgcactcgc ggaagcggca gcgggcctcg gcgcggacgt
    11761 gaccctggtg tccgggccca ccgcgctccc gccgcctgcg aacgcgcact tcgtctacgt
    11821 gacgagcgcg gcggagatgc tcaatgcggt gaacgcgcac gcgtcctccg ccgacctctt
    11881 cttcagcgtc gccgccgtgg cggactacac gcccgaggcg ccggccacgc gcaagatcaa
    11941 gaagtcgggc gagcctctcg agatcaggct caagcccacg gtggacatcc tcgcgcaggt
    12001 ggcgaagatg cccggcgcgc cgttctgcgt gggattcgcc gccgaaagcg agaacctggc
    12061 ggaatacgcg caggccaagc gcaagcgcaa gggcattccg atgatcgtcg cgaacctcgt
    12121 gcagcacacc atcggccgcg acgagaacga ggtcacgatc tacgacgacg cgggcgccca
    12181 ccccatcgcg cgcgcgccga agtcgcgcat cgcgcatgcg gtcctcgagc acgccatcgc
    12241 gctcttcgag gccaagcacg gcggcgcgac cgtcacgccc atcaagcacg tttcgtgaat
    12301 cgcgagtgga ttggcgttat cgcctccagg cgaggagaga cctttggaac acgggcaaat
    12361 ggagagtgat gcgggcgcaa acctttggaa cacggatgaa cacggatcaa cacggatgaa
    12421 cacggatgga tcggattgtc gggaaatgcc atggaagtcc cgggcattga cgacctgatt
    12481 gcgatcactc aaccagccca tgaattatcc gtgttcatcc gtgttcatcc gtgttccaaa
    12541 gattttcgcc aaccgtagcc accaccaccc catgaagaaa cttgacgtga agatcctcga
    12601 cgagcgcatg cgctcgcagc tcccggcgta cgccaccagc ggttccgcgg gcctcgacct
    12661 gcgcgcctgc gtggacgagc cgctgacgct tcacccgggc gactccgccc tcgtgcccac
    12721 cgggctcgcc atccacgtcg ccgacgccgg ccacgccgcg gtgatcattc cgcgctccgg
    12781 cctcggccac aggcacggca tcgtgctcgg caacctcgtc gggctcatcg attccgacta
    12841 ccaggggcag cttctcgtgt cgtgctggaa ccgcggcagg gaaacgttcg tcgtgaaccc
    12901 gatggagcga atcgcgcaac tcgtcgtgat ccccgtggtg caggtggagc tcaacgtggt
    12961 ggagtcattc gacgaaagct cccgcggcga aggcggcttc ggctccacgg gcaagcattg
    13021 aaggcggcca tgcacggcat cgtcctcttc gcccatggcg cccgcgatcc cgaatgggcc
    13081 aggcccttcg aggccatccg cgatcgcgtg cgtgcgcgcc ggcccgagtt tcccgtcgag
    13141 ctcgcgttcc tcgatttcat gactccccat ctcgacgagg ccgtgcatgg tctcgcggcg
    13201 aagggagtcg cggcgatcac catctttccg ctcttcatgg cgccgggagg gcacatcagg
    13261 aacgacctgc cgcgcatcgt cgaggacctg cgcaaggcgc accccggcgt gcccatcgcc
    13321 atccagaccg cggtgggcga agttcccgaa atcctcgaag cgattgcggg ctgggtgctt
    13381 gccggcgtcg actagcctag cgccgggaac agatcgagaa gccgtcgttc cagcccatgc
    13441 ggtattggtc gtcggcggca aaccgcttcg cgtccttgcg gccaaactct gacttcttcg
    13501 ccgattcgca gccgtcgatg tagccgtcgc gcacgggcgg tgggtagccg gtgaggttgt
    13561 aggttggacg cggcgccttc gtgcgccgct ccttcgcctg ctccggcgtt tcctccttcg
    13621 gcggcgaagg ctggatcacc gcgcaggaag ccagcaggaa ggggaaaaac aggatcgccg
    13681 gtcggcgcat cggttgcctc g
//
LOCUS       DASUEV010000046         6420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_816179, whole genome shotgun sequence.
ACCESSION   DASUEV010000046 DASUEV010000000
VERSION     DASUEV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816179"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="hflC"
                     /locus_tag="VFV55_01565"
     CDS             <1..417
                     /gene="hflC"
                     /locus_tag="VFV55_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX4943007.1"
                     /translation="KDVKSIGVEVVDVRLKRVDLVPEISSDVFRRMESERKRAANELR
                     STGSAEGEKIKADADRQKQIIVAEAYRDAQRIKGEGDAQATRIYAEAYGRNAEFYSFY
                     RSLEAYRASLRNKGDVMVLEPTSDFFKYLKNPGRGK"
     gene            430..615
                     /locus_tag="VFV55_01570"
     CDS             430..615
                     /locus_tag="VFV55_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX4943008.1"
                     /translation="MTEILFAGFALMLVIEGILPFVAPKLWREAFRNLIELKDGQIRF
                     AGLVSMSVGLALLVFVA"
     gene            612..1784
                     /locus_tag="VFV55_01575"
     CDS             612..1784
                     /locus_tag="VFV55_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX4943009.1"
                     /translation="MRRWLLPEHIEDILPAEARTVERLRRALLDLLAVHGYELVQPPL
                     LEYTESLLSGTGGDLDLATFRLGDRLSGRQLGIRADHTPQVARIDAHLLNCEGVTRLA
                     YCGSVLHTLPAGMTTTREPIQIGAELYGHAGLEADLEVLRLMSRALSVAGLDKLHLDI
                     GHPRIYGALTAGAGLVVDESEALFHAVQQKDAPAVLAMHDLLGEQRAEAIARLTRLNG
                     DVAVLDEARKDLPKNPDISQALDELARLAKQVAAPGLEVSFDLAELGGFNYESGVVFA
                     AFSPASPDALARGGRYDEVGRSFGRARPATGFTMDLRRIASLVGPTPRPAAILAPCVE
                     DHALDEEVARLRAAGEIVIADLPGHAASRAELGCDRILESKGGRWSVVPLKGNRDG"
     gene            1777..3069
                     /locus_tag="VFV55_01580"
     CDS             1777..3069
                     /locus_tag="VFV55_01580"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX4943010.1"
                     /translation="MAKNVVVIGTQWGDEGKGKIVDWLTDHCAAVVRFQGGHNAGHTL
                     VINGKKTIVRLIPSGIMHPGIKCFIGNGVVVSPEAIMKEIEELEAAGVGVRGRLRISE
                     ACPLILPHHVALDAARERAKGEAKIGTTGRGIGPAYEDKVARRAIRVQDLYARERFAA
                     KLGEVLDYHNFVLKNYFKADVVDFQKTLEDTLALGEKLQPMVADVSGEVNALIREGKS
                     ILFEGAQAALLDVDHGTYPFVTSSNCVAGQASAGAGVGPQQLHYILGVAKAYATRVGG
                     GPFPTELDDEVGEYLRQKGNEYGAVTGRPRRCGWFDAAALKRAVQLNGLSGLCITKLD
                     VLDGLDTVRLSVGYKVRGELRDILPVGAEALAICEPVYEDFPGWKESTVGARSFDELP
                     ANARSYLKRLESLAGAPIVLISTGPDREETIVLQHPFG"
     gene            complement(3082..4092)
                     /locus_tag="VFV55_01585"
     CDS             complement(3082..4092)
                     /locus_tag="VFV55_01585"
                     /inference="COORDINATES: protein motif:HMM:NF037752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HEX4943011.1"
                     /translation="MSFTHRRVAAATLCALVLATSVGPAFAAPFALPQVEIPGSDLTN
                     WVIVNDGGTSTGAPFTGTCSLAPGLSIVDAGGPLDANDAYDNAYNLYVNGAAFVAPPL
                     VDFTGTTMTAGPVAMSGLNVTVQYYFSPLSAVARLLYTMQNPTDAPIAATVQIPVNFG
                     SDAGTVIETTSSGDVVVSTADRWVVSSDGVPGDSVNLTVMAGPGAVAVPLSAVTRTVF
                     TCSGAQGLGMTYNLNVPAFASQSVMLFAGLEGVNANNNTVASATTAAALFDSNATLQA
                     DWLDGMNAGQLAQVVNWAFPAVVAAQPIPTLSELAMVVLSAMMGAVAFLAIRRRRGSG
                     TA"
     gene            complement(4310..4394)
                     /locus_tag="VFV55_01590"
     tRNA            complement(4310..4394)
                     /locus_tag="VFV55_01590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4358..4360),aa:Leu,seq:cag)
     gene            4538..>6420
                     /gene="rnr"
                     /locus_tag="VFV55_01595"
     CDS             4538..>6420
                     /gene="rnr"
                     /locus_tag="VFV55_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX4943012.1"
                     /translation="MILEVLDREGVPLTEDALASLLGIEAHERDSFGRRLAAMEREGQ
                     MMRNRRGAILVADKAGLIKGRVMGHPDGFGFLKPDDASEDLFLGPKQMHKALHGDIVL
                     ARVTGLDRRGRREGSIVEVLERANSRVVGRLFVEQGVVFIAAEDRRIAQDFLVPPDGA
                     KGAKHGQVVVAEIVQQPTPNAEPIARVVEILGNYADPGMEIEIALRKHDLPHVFSGES
                     EKQAAKFGSKVLAKDLKGRKDLRDLPFVTIDGETARDFDDAVCCQPIAKGKSFRLWVA
                     IADVSHYVRPKDALDNESRERGNSVYFPRRVIPMLPEALSNELCSLKPEVDRLTMVCE
                     MEVTNGGVVRTYAFYPAVIHSHARLTYTRVAAVLEGIEAEPPVDKALVPSLEHLYAVF
                     KSLLGARAKRGAIDFDSVETQMIFDDKGKIERIVRVQRNDAHRLIEECMLAANVCASD
                     YLLQNNQPTLYRVHEGPTPEKLEALRSMLKDFGLVLGGGDEPQAMDYAKLLDRIKDQP
                     YAGLLQTVMLRSLRQAVYSPKNVGHFGLAYEGYTHFTSPIRRYPDLLVHRAIKAVLAG
                     STYDAGDWAALGVHCSETERRADDATRDVENWLKCFFMQDHVGDEFEGTVSGVAGFGL
                     FVTL"
BASE COUNT         1093 a         2129 c         2218 g          980 t
ORIGIN      
        1 aaggacgtga agagcatcgg cgtggaagtg gtggacgtgc ggctcaagcg cgtcgacctc
       61 gtgcccgaga tctcctccga cgtcttccgc cgcatggagt ccgaaaggaa gcgcgcggcc
      121 aacgagcttc gctccacggg aagcgccgag ggcgagaaga tcaaggcgga cgccgaccgg
      181 cagaagcaga tcatcgtggc cgaggcctac cgcgacgcgc agcgcatcaa gggcgagggc
      241 gacgcgcagg ccacgcgcat ctacgccgag gcctatggcc ggaacgccga gttctacagc
      301 ttctaccgca gcctcgaggc ctaccgcgcg tcgctgcgca acaagggcga cgtgatggtg
      361 ctcgagccca cctcggactt cttcaagtac ctgaagaacc cgggccgcgg caagtagggc
      421 gttcgggcgt tgacggaaat cctgttcgcg ggttttgcgc tcatgctcgt gatcgaaggc
      481 atccttcctt tcgtagcgcc caagctctgg cgcgaagcct tccgcaacct gatcgagctg
      541 aaggacgggc agatccgctt cgcgggcctg gtgtcgatgt ccgtcgggct ggccctgctg
      601 gtgttcgtgg catgaggcgc tggctgcttc ccgagcacat cgaggacatc ctccccgccg
      661 aggcgaggac ggtggagcgc cttcgccgcg ccctcctgga cctcctcgcc gtgcacggct
      721 acgagctggt gcagccgccg ctcctcgaat acaccgagtc gctgctctcc ggcacgggcg
      781 gcgacctgga cctcgccacg tttcgcctcg gcgatcgtct ctcggggcgg cagctgggca
      841 tccgcgcgga ccacacgccg caggtggcgc gcatcgacgc gcaccttctc aactgcgagg
      901 gcgtgacgcg gctcgcctat tgcggcagcg tgctgcacac gctgcccgcg ggcatgacca
      961 cgacgcgcga gccgatccag atcggcgccg agctgtacgg ccacgcgggg ctggaggcgg
     1021 atctcgaggt gctgcggctc atgtcgcgcg cgctttcggt cgccgggctc gacaagctgc
     1081 acctggacat cggccacccg cgcatctacg gggcgctcac cgcgggcgcc ggcctcgtgg
     1141 tcgacgagtc cgaggcgctc ttccacgccg tgcagcagaa ggacgcgccg gcggtgctcg
     1201 cgatgcacga cctgctcggc gagcagcgcg cggaggcgat cgcgcgcctg acgcgcctga
     1261 acggcgacgt ggccgtgctg gacgaggcgc gcaaggacct gccgaagaac ccggacatct
     1321 cgcaggcgct ggacgagctc gcccgcctgg cgaagcaggt tgccgccccc ggcctggagg
     1381 tgagcttcga cctggccgag ctgggcggct tcaattacga aagcggcgtc gtgttcgcgg
     1441 ccttctcgcc ggcctcgccc gatgcgctgg cccgcggcgg gcgctacgac gaggtggggc
     1501 gctccttcgg gcgcgcgcgg cccgccaccg gcttcaccat ggatcttcgc cgcatcgcct
     1561 cgctcgtcgg ccccacgccg cgtccggccg ccatccttgc gccctgcgtc gaggaccacg
     1621 ccctcgacga ggaagtggcg cgactgcgag cggcgggcga gatcgtcatc gcggaccttc
     1681 ccggccacgc cgcctcgcgc gcggaactgg gctgcgaccg tatcctggaa tccaagggag
     1741 gccgctggtc ggtcgtcccg ttgaaaggca accgagatgg ctaagaacgt cgtggtgatc
     1801 ggcacgcaat ggggcgacga gggcaagggc aagatcgtcg actggctcac ggaccattgc
     1861 gcggccgtcg tgcgcttcca gggcgggcac aacgccgggc acacgctggt gatcaacggc
     1921 aagaagacga tcgtgcgcct catcccctcg gggatcatgc acccgggcat caagtgcttc
     1981 atcggcaacg gcgtggtggt ctcgcccgag gcgatcatga aggagatcga ggaactcgag
     2041 gcggccgggg tgggcgtgcg cggccggctg cgcatctctg aggcgtgccc gctgatcctg
     2101 ccccaccacg tggcgctgga cgcggcgcgc gaacgggcga aaggcgaggc gaagatcggc
     2161 accaccgggc gcggcatcgg gccagcctac gaggacaagg tggcgcgtcg cgccatccgc
     2221 gtgcaggacc tctacgcgcg cgagcgcttc gccgccaagc tgggcgaggt gctggattat
     2281 cacaacttcg tcctgaaaaa ttacttcaag gctgacgtgg tggatttcca gaagacgctg
     2341 gaggacacgc tggccctcgg cgagaagctg cagcccatgg tggccgacgt ctccggcgag
     2401 gtgaacgcgc tcatccgcga aggcaagagc atcctcttcg agggcgctca ggccgcgctg
     2461 ctggacgtgg accacggcac ctatccgttt gtcacctcat ccaattgcgt ggcggggcag
     2521 gcgtcggccg gggcgggcgt gggcccgcag cagctgcact acatcctggg cgtggccaag
     2581 gcctacgcga cgcgcgtggg gggcgggcct ttcccgacgg agctcgacga cgaggtcggc
     2641 gagtaccttc gccagaaggg caacgaatac ggtgctgtca ccgggcggcc gcggcgctgc
     2701 ggctggttcg atgcggcggc cctcaagcgc gcggtgcagc tgaacgggct ctccggcctt
     2761 tgcatcacca agctcgacgt gctggacggc ctggatacgg tgcgcctttc cgtcggctac
     2821 aaggtgcgcg gcgagttgcg cgacatcctg ccggtgggcg ccgaggcgct tgccatctgc
     2881 gagccggtgt acgaggattt tccgggatgg aaggagagca cggtcggcgc caggtccttc
     2941 gacgagctgc cggccaacgc gcgcagctat ctcaagcgcc tggagtcgct ggccggcgcg
     3001 cccatcgtgc tcatctccac cggccccgat cgcgaagaga cgatcgtgct gcagcacccc
     3061 ttcggctagg ggcagcgcct gtcaggcggt gcccgaaccg cggcggcggc ggatcgccag
     3121 gaacgcaacg gcgcccatca tcgcggagag cacgaccatc gccaactccg agagcgtcgg
     3181 gatgggttgc gccgcgacca ccgcagggaa cgcccagttg accacctgcg cgagttgccc
     3241 cgcattcatt ccgtccagcc agtcggcctg aagggtggca ttggaatcga agagggcggc
     3301 tgcggtcgtt gccgaggcga cggtgttgtt gttggcattc acgccttcca gcccggcgaa
     3361 cagcatgacc gactgcgacg cgaacgcggg cacgttcagg ttgtaggtca tcccgaggcc
     3421 ctgggcgccg gagcaggtga agaccgtccg ggtgacggcg gagagcggca cggcgaccgc
     3481 gccagggccg gccatgacgg tgaggttcac ggagtcgccc ggcacgccgt cggaagagac
     3541 gacccagcgg tccgcggtgc tcaccaccac atcgccggat gaggttgttt cgatgaccgt
     3601 gccggcgtcg gagccgaagt tgacgggaat ctggactgtg gcggcaatcg gcgcgtccgt
     3661 ggggttctgc atcgtgtaca gcaggcgcgc cacggcgctc aagggagaga agtaatactg
     3721 caccgtcacg ttgaggcccg acatcgcgac cggcccggcg gtcatcgtgg tgccggtgaa
     3781 gtcgacgagg gggggagcca cgaatgccgc gccgttcacg tacaggttgt aggcgttgtc
     3841 gtaagcgtcg ttcgcgtcca gggggccccc cgcatcgacg atcgaaagcc ccggcgcgag
     3901 agagcatgtc cccgtgaagg gcgcgccggt ggatgtaccc ccgtcgttga cgatgaccca
     3961 gttcgtaagg tcggagccgg ggatctctac ctgcgggagg gcgaacgggg cggcgaaggc
     4021 ggggccaacg gacgtggcga gaaccagcgc gcaaagggtt gccgcagcga ccctgcggtg
     4081 cgtgaaggac atggaattcg ctccctggct tggaattgtt cttgcgctcc ctccaacagc
     4141 actcatgact caagggaagc gaaaaccatt gtagccacgc cgcgatcgcc cgcaaaggcg
     4201 cgcatggtaa aattcttcct cttgtgcatc gcgaaaaacc aaaggccgcc cggcctttcg
     4261 gcgggcggcc tttcggatgc cgcaatcact ctcgcaaagc gaaaaaatct ggtgcccgga
     4321 agaggactcg aacctccacg gtgttacccg ccagtacctg aaactggtgc gtctaccaat
     4381 tccgccatcc gggcatggcc atgcgtcaat caagccaagc ccataatttt agaaggaaga
     4441 cacttttttg tccagtaaaa ccaagaccct gcgtcacgcg gatccccacc gggaccgcga
     4501 agccgccaag tacgaaagcc cgctgcccag ccgcgagatg atcctcgagg tgctggaccg
     4561 cgaaggcgtt cccctcacgg aggacgcact cgcctccctc ctcggcatcg aggcgcacga
     4621 gcgcgactcc ttcggccggc gcctggccgc catggagcgc gaaggccaga tgatgcgcaa
     4681 ccgccgcggc gcgatcctcg tcgcggacaa ggccggcctc atcaagggcc gcgtcatggg
     4741 ccacccggac ggcttcgggt tcctcaagcc cgatgatgcc tccgaggacc tgttcctcgg
     4801 cccgaagcag atgcacaagg cgctgcacgg cgacatcgtg ctcgcccgcg tgaccggcct
     4861 ggaccgccgc ggccgccgcg aaggctccat cgtcgaagtg ctcgagcgcg ccaacagcag
     4921 ggtggtgggg cgccttttcg tcgagcaggg cgtggtgttc atcgcggcgg aggacaggcg
     4981 catcgcgcag gatttcctgg tgcctccgga cggcgccaag ggcgcgaagc acgggcaggt
     5041 ggtggtggcc gagatcgtcc agcagcccac gcccaatgcc gagcccatcg cgcgcgtggt
     5101 ggagatcctc ggcaactacg ccgacccggg gatggaaatc gagatcgcgt tgcgcaagca
     5161 cgacctgccg cacgtgttct cgggtgaatc cgagaagcag gcggcgaagt tcggcagcaa
     5221 ggtgctggcc aaggacctca agggccgcaa ggacctgcgc gatcttccgt tcgtgacgat
     5281 cgacggggag acggcccggg atttcgacga tgcggtgtgc tgccagccga tcgccaaggg
     5341 aaaatccttc cgtctctggg tggccatcgc cgacgtgagc cactacgtgc gcccgaagga
     5401 cgcgctggac aacgaatcgc gcgagcgcgg caactcggtg tacttcccgc gccgcgtgat
     5461 cccgatgctg cccgaggcgc tctccaacga gctctgctcg ctcaagcccg aggtggatcg
     5521 cctcacgatg gtgtgcgaga tggaagtcac caacggcggc gtggtgcgca cctacgcgtt
     5581 ctacccggcc gtcatccact cgcacgcgcg cctcacctat acgcgcgtgg cggcggtgct
     5641 tgaaggcatc gaagccgagc cgcccgtcga caaggcgctc gttccgtcgc tcgagcacct
     5701 gtacgcggtc ttcaagtcgc tgctcggcgc gcgggccaag cgcggggcca tcgacttcga
     5761 ttccgtcgag acgcagatga tcttcgacga caagggcaag atcgagcgca tcgtgcgcgt
     5821 ccagcgaaac gacgcgcacc gcctcatcga ggagtgcatg ctggcggcca acgtgtgcgc
     5881 gtcggactac ctgctgcaga acaaccagcc gacgctctac cgcgtccacg aggggcccac
     5941 gccggagaag ctggaggcgc ttcgctccat gctgaaggac ttcggcctcg tgctcggcgg
     6001 cggtgacgag ccgcaggcga tggactacgc caagctgctg gaccgcatca aggaccagcc
     6061 ctacgcggga ctgctgcaga cggtgatgct gcgctcgctg cgccaggcgg tgtacagccc
     6121 caagaacgtc ggccacttcg gcctggccta cgagggctac acgcacttca cctcgcccat
     6181 ccgccggtat ccggaccttc tcgtgcatcg cgcgatcaag gcggtgctcg ccgggtcgac
     6241 ctacgatgcc ggtgactggg ccgcgctggg cgtgcactgc tccgagaccg agcgccgcgc
     6301 cgacgatgcc acgcgcgacg tcgagaactg gctcaagtgc ttcttcatgc aggatcacgt
     6361 gggcgacgag ttcgagggca cggtgagcgg cgtggcgggc ttcggcctgt tcgtcacgct
//
LOCUS       DASUEV010000047         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_816530, whole genome shotgun sequence.
ACCESSION   DASUEV010000047 DASUEV010000000
VERSION     DASUEV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816530"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VFV55_01600"
     CDS             <1..1231
                     /locus_tag="VFV55_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX4943013.1"
                     /translation="ILRDLEDGAVKFLWVQVTNPFQSSPNANHWMKAAREMDNFIVVA
                     DCYPTVSGKVADLVLPAAMIFEKWGAYGNAERRTQMWRQQVDAPGEARTDIWMMLEFA
                     RRFKVGEVWGEKNLPGLKTEGFADGKLPDVLEAAKALGYKPEQSLYEALFATPENRKF
                     AWPDPVSKGKSNATVKALGENWFVEKALFEEYAQFGRGHHHDLADFDMYFRDDVRGLR
                     WPVVNGKETKYRFVEGHDPYVKAGEGFNFYGGAFKALATGTLDDVTDPKPTAFPGKAK
                     IFFRPYAAPVEQPDKAYDLWLCTGRILEHWHTGSMTRRVSELNRAAPSAVLYMHPKDA
                     ESRGIKRNDTVWIESRRGKIKLVVDTRGRNQMPRGSVFAAFFDESLLINKLVLDAVDP
                     ISRETDFKKCAVKVLKA"
     gene            1263..2048
                     /gene="napG"
                     /locus_tag="VFV55_01605"
     CDS             1263..2048
                     /gene="napG"
                     /locus_tag="VFV55_01605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HEX4943014.1"
                     /translation="MAVAGSGRRQFLTHAVQGIAAAACGGVAWNALLVQQVHAVPFAL
                     RPPGAQAEPDFAATCVKCGACVVACPYGTLKLAKAATGIPLGTPHFVAREEPCRLCPG
                     IPCAKACPTGALDRDMSDINKSRMGLAVVDAENCLSWNGLRCEVCYRVCPLQGKAISI
                     DPHPRKLSKHAVFVPVVHSDACTGCGLCERACPTTVSAVRIVQPALVQGRMGDHYRME
                     WRPEAATEPPESPRAAPPAAAKPSPNAPDKAPGLDYFNAEAKP"
BASE COUNT          366 a          728 c          727 g          318 t
ORIGIN      
        1 gatcctgcgc gacctcgagg acggggccgt caagttcctc tgggtgcagg tgaccaaccc
       61 gttccagtcc tcgcccaacg ccaaccactg gatgaaggcc gcgcgcgaga tggacaactt
      121 catcgtcgtg gccgactgct accccacggt atcgggcaag gtcgcggacc tggtgctgcc
      181 ggcggcgatg atcttcgaga agtggggcgc ctacggcaac gccgagcggc gcacgcagat
      241 gtggcgccag caggtggacg cgccgggaga agcgcgcacg gacatctgga tgatgctcga
      301 gttcgccagg cgcttcaaag tgggcgaagt gtggggcgag aagaacctcc cgggcctcaa
      361 gacggagggc ttcgccgacg gcaagctccc cgacgtgctg gaagcggcaa aggccttggg
      421 ctacaagccc gagcagtcgc tctacgaggc gctcttcgcc acgccggaga accggaaatt
      481 cgcctggccc gacccggtgt ccaagggcaa gtccaacgcg acggtgaagg cgctgggcga
      541 gaactggttc gtcgagaagg cgctcttcga ggaatacgcg cagttcggcc gcggccatca
      601 ccacgacctc gcggatttcg acatgtactt ccgcgacgac gtgcgcgggc tgcgctggcc
      661 ggtcgtcaac ggcaaggaaa cgaagtaccg cttcgtcgag ggccacgacc cgtacgtgaa
      721 ggcgggcgaa ggcttcaact tctatggcgg cgccttcaag gcgctcgcca ccggcactct
      781 cgacgacgtg accgatccca agcccacggc gttcccgggc aaggcgaaga tcttcttccg
      841 gccgtatgcg gcgcccgtcg agcagccgga caaggcctac gacctgtggc tgtgcacggg
      901 gcgcatcctc gagcactggc ataccggctc catgacgcgc cgggtatccg agctcaatcg
      961 cgccgcgccc tcggccgtgc tctacatgca cccgaaggat gcggaaagcc gcgggatcaa
     1021 gaggaacgac accgtctgga tcgagtcgcg ccgcggcaag atcaagctgg tcgtggacac
     1081 caggggccgc aaccagatgc cgcgcggctc cgtgttcgcg gctttcttcg acgagtcgct
     1141 gctcatcaac aagctggtcc tcgacgccgt ggacccgatc tcgcgcgaaa ccgacttcaa
     1201 gaagtgcgcg gtgaaggtct tgaaggcctg aggccccagg acatcaccgg ggatgacgtg
     1261 acatggccgt tgcgggatcc gggcgccggc agttcctcac gcacgccgtg cagggcatcg
     1321 ccgcggcggc gtgcggtggc gtcgcgtgga atgcgcttct cgtgcagcag gtgcacgcgg
     1381 tgccgttcgc gcttcgcccg ccgggcgcgc aagccgagcc cgacttcgcc gccacgtgcg
     1441 tgaagtgcgg cgcctgcgtg gtggcctgcc cctacggcac gctcaagctc gccaaggcag
     1501 caaccggcat cccgctgggc acgccccact tcgtcgcgcg cgaggagccg tgccggcttt
     1561 gtcccgggat tccgtgcgcg aaggcctgcc cgaccggggc gctcgaccgc gacatgtcgg
     1621 acatcaacaa gtcgcgcatg ggcctcgccg tcgtcgacgc cgagaactgc ctgtcgtgga
     1681 acggcctgcg ctgcgaggtg tgctatcgcg tctgcccgct gcagggcaag gcgatctcca
     1741 tcgacccgca tccgcgcaag ctctcgaagc acgcggtatt cgtgccggtg gtccattcgg
     1801 acgcgtgcac cggttgcggc ctgtgcgagc gcgcctgtcc caccacggtg tccgcggtgc
     1861 gcatcgtgca gccggcgctc gtgcagggac gaatgggcga tcactaccgc atggaatggc
     1921 gaccggaggc ggccacggaa ccgcccgaat cccctcgggc cgcgccgccc gcagccgcga
     1981 aaccttcacc gaacgcgccg gacaaggcac cggggctcga ctacttcaat gccgaggcga
     2041 aaccgtgagc gcgaacggcc gacccgtgcg ccacttcgag atgccctcgt cgcttgcggg
     2101 caaggtgcac gcctggcgct tcctgatcgc gcggcgcgc
//
LOCUS       DASUEV010000048         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_587826, whole genome shotgun sequence.
ACCESSION   DASUEV010000048 DASUEV010000000
VERSION     DASUEV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587826"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFV55_01610"
     CDS             complement(<1..295)
                     /locus_tag="VFV55_01610"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEX4943015.1"
                     /translation="MPDSLHSLEEAITTDVHRALAEDVGTGDLTAALLPPDRIVRARL
                     LTRQDAVLCGTEWFNRTVEALDPEAEVFWHHADGDELVAGNSLCEVEANVRALL"
     gene            418..3024
                     /gene="clpB"
                     /locus_tag="VFV55_01615"
     CDS             418..3024
                     /gene="clpB"
                     /locus_tag="VFV55_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX4943016.1"
                     /translation="MRIDKLTTKFQQALADAQSAALAHDSGFVEPQHLLLALLNQQDG
                     GSASLLQRAGVNVASLRKALEDSVARLPKVEGQGGEMTVSRDLGNLLNVTDREATKRG
                     DQYIASELFLLAAVQDKGETGRLLKQHGATAAAIDTAIKAVRGGQAVEDPEAEGSREA
                     LKKYTLDLTERARQGKLDPVIGRDDEIRRTIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPETLKDKRVLSLDMASLLAGAKYRGEFEERLKSVLKEIAQDEGRIIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVESTIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDTKPESMDKLDRRLIQLKIEREAVKKEKDEASKKRLELLEAEIAR
                     LGKEYADLEEVWSAEKHAVHGSAAIKEEIEKLKLAMEEARRKGDWARMSELQYGKLPQ
                     LEAQLGKADKAQGAAAKPKLLRTEVGAEEIAEVVSRATGIPVSRMMQGEREKLIQMEA
                     KLHERVVGQDEAVRLVADAIRRSRAGLSDPDRPYGSFLFLGPTGVGKTELCKALAAFL
                     FDSEDHLIRVDMSEFMEKHSVARLIGAPPGYVGYEEGGHLTEAVRRKPYSVILLDEIE
                     KAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQSMAGSDPSLIKM
                     SVMAEVKSQFRPEFVNRIDEIVVFHALDEAHIRNIAKIQLRYLTDRLAKLEMHLEVSD
                     SALAQLATAGFDPVYGARPLKRAIQQAIENPLAKEILEGRFAAKDTILVGHGKGGFAF
                     TKAGAKAKVAAI"
     gene            complement(3044..3553)
                     /locus_tag="VFV55_01620"
     CDS             complement(3044..3553)
                     /locus_tag="VFV55_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEX4943017.1"
                     /translation="MLKKPLAIAAIATAAAAALVPTQANAGDPALGAILGATLGAAIG
                     HNVNGHHGAVVGGVLGAVTGASIAADSRGYYDSGYYDAPPAYYPPAPVYYDAPRAYYP
                     PAPVYYAPPPVVIRTRPVFVHNYVPHRPAYASVVVERRHDWRHDWRHDGRYGSRDQRG
                     WRDGDRRWR"
     gene            3888..5054
                     /locus_tag="VFV55_01625"
     CDS             3888..5054
                     /locus_tag="VFV55_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX4943018.1"
                     /translation="MPESPPGGFALWNLGFRPFYLLASIFSAASIAVWAAQYAGWLPA
                     GAYLKGHLWHAHEMLFGYTVAVVAGFLLTAVRNWTNQTTPSGMLLAGLAALWVAGRVL
                     ALAPWPVAAAVVNAAFPVAIAVAIAVPMVRSANRRNYFFAALLAFMGVAVLAFHLSNQ
                     GTLSLPPRLGLLAGLDLVLFIMVVMGGRVIPMFTNNGVPGTNARRTPATERFALGSVL
                     VLLAADLAQVPAAVVGTVAVAAAIAHAVRLALWQPWRTLHTPLVWVLHGAYAWIVVHL
                     ALRALAAAGLVGESLALHALTLGAIGGLTIGMMTRTAKGHTGRQLKADGYEVAAYGLV
                     MAAALVRVAGGILLPGAYPATVAGSAALWSAAFAIYAVRYWPVLTRPRIDGKPG"
     gene            5064..5558
                     /gene="xrtH"
                     /locus_tag="VFV55_01630"
     CDS             5064..5558
                     /gene="xrtH"
                     /locus_tag="VFV55_01630"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HEX4943019.1"
                     /translation="MKRFAILFAVILLVLFALELTAPGQAIVKPWTQFVASTSSELIR
                     AFDGDAKAKGIMLYSERTGFAVTIEAGCNGVEAMLVLLAGILAFPAPWRHKFIGLAIG
                     AVAIQALNILRVVSLFYLGQWDRQWFDWAHLYVWQSLIMLDAMIVWLLWIRAIPARSS
                     SVAA"
BASE COUNT          965 a         1841 c         1988 g          900 t
ORIGIN      
        1 tgaggagcgc gcggacgttc gcctcgacct cgcacaggga attgccggcc accagttcgt
       61 cgccgtcagc gtggtgccag aagacttcgg cctcgggatc gagggcttcc acggtgcggt
      121 tgaaccactc ggtgccgcag agcacggcgt cctggcgggt caggagccgg gcgcgcacga
      181 tcctgtccgg gggcaacagg gccgcggtga ggtccccggt gccgacatcc tcggccagcg
      241 cgcggtggac atcggtggtg atcgcttctt cgagggagtg gagggaatcg ggcatggcag
      301 gggaggcgtg gcagggggaa aatacttgaa acgggccggg tttgccccat tttcgtgttt
      361 gaacccgcga tatccgggag aattcccgag aaaatccgtt ggaaaaggat accaccgatg
      421 cgcatcgaca agctgaccac gaaattccag caggccctcg ccgacgccca gagtgcggcg
      481 ttggcccacg actccggatt cgtcgagccg cagcacctgc tcctcgccct cctgaaccag
      541 caggacgggg gcagcgcgtc gctcctgcag cgcgcggggg tgaacgtggc gtcgctgcgc
      601 aaggccctcg aggattcggt ggcccggttg ccgaaggtgg aaggacaggg cggcgagatg
      661 accgtctcgc gcgacctggg aaatctcctc aacgtcaccg accgggaagc gaccaagcgg
      721 ggcgaccagt acatcgcctc cgagctcttc cttctggctg ccgtccagga caagggcgag
      781 accgggcggc tcctcaagca gcatggcgcc acggccgcag ccatcgacac cgcgatcaag
      841 gccgtgcgcg gcggccaggc cgtcgaggac ccggaagccg agggcagccg cgaggcgctc
      901 aagaaataca cgctcgacct caccgagcgc gcacggcagg gcaagctcga ccccgtcatc
      961 ggccgcgacg acgagatccg ccgcaccatc cagatcctgc agcgccggac caagaacaac
     1021 ccggtgctca tcggcgagcc cggcgtgggc aagaccgcga tcgtcgaggg ccttgcccag
     1081 cgcatcgtga acggcgaagt gcccgagacc ctcaaggaca agcgcgtcct ttcgctcgac
     1141 atggcttcgc tccttgccgg cgccaagtac cgcggcgaat tcgaggagag gctcaagtcc
     1201 gtcctcaagg agatcgcgca ggacgagggc cgcatcatcg tgttcatcga cgagctgcac
     1261 acgatggtcg gggcgggcaa ggccgaaggg gccatcgacg ccggcaacat gctcaagccc
     1321 gcgctcgccc gcggggagct gcactgcgtg ggcgccacca cgctcgacga ataccgcaag
     1381 tacatcgaga aggacgccgc gctcgagcgg cgcttccaga aggtgctcgt cgacgagccg
     1441 agcgtcgaat cgacgatcgc gatcctgcgc ggcctgcagg agaagtacga ggtgcaccac
     1501 ggcgtggaca tcaccgaccc ggccatcgtc gccgcggccg agctttcgca ccgctacatc
     1561 accgaccgtt tccttcccga caaggcgatc gacctgatcg acgaggcggc cgcgcgcatc
     1621 aagatggaga tcgacacgaa gcccgagtcg atggacaagc tcgatcgccg gctcatccag
     1681 ctgaagatcg agcgcgaggc ggtgaagaag gagaaggacg aggcctcgaa gaagcgcctc
     1741 gagctgctcg aagccgagat cgccaggctc ggcaaggaat acgcggacct cgaggaggtc
     1801 tggagcgccg agaagcacgc ggtgcacggc tccgcggcga tcaaggagga gatcgagaag
     1861 ctcaagctcg ccatggaaga ggcgcgccgc aagggcgact gggcgaggat gagcgagctg
     1921 cagtacggga agctgccgca gctcgaggcg cagctgggca aggccgacaa ggcccagggc
     1981 gcggcggcga agcccaagct cctgcgcacc gaagtgggcg cggaggaaat cgccgaggtg
     2041 gtgtcgcgcg ccaccggtat ccccgtctcc cggatgatgc agggcgagcg ggaaaagctg
     2101 atccagatgg aggcgaagct gcacgagcgc gtcgtgggcc aggacgaggc cgtgcgcctc
     2161 gtggccgacg ccatccgtcg ctcgcgcgcg gggctttcgg atcccgatcg cccctatggc
     2221 tcattcctgt tcctgggacc gacgggggtg ggcaagaccg agctgtgcaa ggcgctcgcc
     2281 gcgttccttt tcgattcgga ggatcacctg atccgcgtcg acatgagcga gttcatggag
     2341 aagcactcgg tagcccgcct catcggcgcg ccacccgggt acgtgggcta cgaggagggc
     2401 ggccacctca ccgaggcggt gcgccgcaag ccctattcgg tgatcctcct cgacgagatc
     2461 gagaaggcgc acccggacgt gttcaacgtg ctgctgcagg tgctcgacga cgggcgcatg
     2521 accgacgggc agggccgcac ggtggacttc aagaacacgg tgatcgtgat gacttcaaac
     2581 ctcggcagcc agatgatcca gtcgatggcg ggcagcgacc cctcgctcat caagatgtcg
     2641 gtcatggccg aggtgaagtc gcagttccgc ccggagttcg tcaaccgcat cgacgagatc
     2701 gtggtcttcc acgccctcga cgaggcgcac atcaggaaca tcgcgaagat ccagctgcgc
     2761 tacctcacgg atcggctcgc gaagctcgag atgcacctcg aggtgtcgga cagcgcgctc
     2821 gcgcagcttg ccaccgccgg cttcgacccg gtctacgggg cgcggccgct gaagcgcgcg
     2881 atccagcaag cgatcgagaa tccgctcgcg aaggagattc tcgaggggcg cttcgccgcc
     2941 aaggatacga tcctcgtcgg ccacggcaag ggagggttcg ccttcacgaa ggcgggggcg
     3001 aaggcgaagg tagccgccat ttgacctgct gccagccgct cggctagcgc cagcggcgat
     3061 cgccatcgcg ccagccgcgc tggtcgcgcg agccatagcg gccatcgtgg cgccagtcgt
     3121 gtcgccagtc gtgtcgcctt tccacgacga ccgaggcata cgcaggccgg tgcgggacat
     3181 agttgtgcac gaacaccggg cgcgtgcgaa tgacgaccgg gggcggcgcg tagtaaacgg
     3241 gcgccggcgg gtagtaggcg cgcggcgcat cgtagtagac gggcgccggc gggtaatagg
     3301 ccggcggggc atcgtagtag ccggagtcgt agtaccccct ggaatcggct gcaatgctgg
     3361 cgccagtcac cgctcccagc acgccgccta ccaccgcacc atggtggccg ttcacgttgt
     3421 gcccgatggc ggcgccgagg gtcgcaccga gtattgcgcc caatgccggg tcgccagcat
     3481 tggcttgcgt cggaaccagg gccgcagctg ccgcggttgc gatggcggca atcgcgaggg
     3541 gtttctttaa cattgtcgtg tcctccgtct cgtggatgat cgggccggaa tcctttgcgg
     3601 ccagtgccgt atttgccggc acctgtagcc actaacgccc gagcgccggg caggtgcaca
     3661 cggagacaat ctgttacaag tcggccgccc gccgcggcgc cgcgttatag tggtcacgtt
     3721 tgggggcata gtgccgttaa aacataacct tggaacaaag gaggctggac gatgagccac
     3781 agcaaggaca aggacacgaa gaagatggtg aagaaggaac ctctgaagac gcccaaggaa
     3841 aagaaggaag ccaagaaact caagaagcag gagcggcaac gcgggtgatg ccggaaagcc
     3901 cgccaggggg cttcgccctc tggaacctgg gcttccggcc cttctacctc ctggcgagca
     3961 tcttcagcgc cgcgagcatc gcggtctggg cggcccagta cgcgggctgg ttgcctgccg
     4021 gggcgtacct gaaagggcac ttgtggcacg ctcacgagat gctcttcggc tatacggtgg
     4081 cggtggtggc cgggttcctg ctcacggccg tgcgcaactg gacgaaccag accacgccga
     4141 gcgggatgtt gctggcgggc ctcgcggcac tatgggtcgc gggacgcgtg ctggcgttgg
     4201 cgccgtggcc ggtggccgcc gcggtggtga acgcggcctt tccggtggcg atcgccgtgg
     4261 ccatcgcggt gcccatggtc aggagcgcca accggcgcaa ctatttcttc gccgccttgc
     4321 tcgccttcat gggcgtcgcg gtgctcgcgt tccatctatc caaccaggga acgctctcgt
     4381 tgccgccccg gctgggcctg ctggccggcc tcgacctcgt gctcttcatc atggtggtca
     4441 tgggcgggcg cgtcattccg atgttcacca acaacggcgt gcccggcacg aacgcgcggc
     4501 gcacgccggc gacggaacgg ttcgcgctcg gcagcgtcct ggtcttgctg gcggcggatc
     4561 tcgcgcaggt tccggcggcg gttgtcggca ccgtggccgt ggcggctgcc atcgcccacg
     4621 cggtccgcct cgcgctctgg cagccgtggc gcacgctgca cacgccgctg gtttgggtgc
     4681 tgcatggcgc ctacgcgtgg atcgtcgtcc acctggcgct gcgcgccctg gcggctgcgg
     4741 gtctcgtggg agagtcgctc gcgctgcacg cgctgaccct cggggccatc ggcgggctca
     4801 ccatcggcat gatgacgcgc accgcgaaag gccacaccgg gcggcagctc aaggcggacg
     4861 gctacgaagt cgcggcctac ggcctggtga tggcggccgc gctggttcgc gtggccggag
     4921 gcatcctcct gcccggcgcc tatccggcga cggttgccgg ttcggcggcg ctttggtcgg
     4981 ccgcgttcgc gatctacgcg gttcgctatt ggcccgtgct cacccgcccc cggatcgacg
     5041 gcaagccggg ttaggattcg cccgtgaagc gcttcgcgat ccttttcgcc gtcatcctgc
     5101 tcgtgctgtt cgcgctcgag ctcacggcgc cgggccaggc gatcgtgaag ccgtggacgc
     5161 agttcgtcgc ctcgaccagc tcggagctga tccgtgcctt cgatggcgac gcgaaggcga
     5221 agggcatcat gctctacagc gagcgcaccg gtttcgccgt caccatcgag gcgggctgca
     5281 acggcgtcga ggcgatgctg gtgctgctgg ccggcatcct cgcctttccg gcgccgtggc
     5341 ggcacaagtt catcgggctc gccatcggcg cggtggcgat ccaggcgctc aacatcctgc
     5401 gcgtcgtgag cctcttctac ctcggccagt gggaccggca atggttcgac tgggcgcatc
     5461 tctacgtatg gcagtccctg atcatgctcg acgcgatgat cgtctggctg ctgtggatac
     5521 gggcgatacc ggctcgttct tcctccgtgg ccgcgtgatg acgcgaccgg agcagcgccg
     5581 gctcctggtt attttcgggc gggtcctgct gttgctgccg ctctttttcg gcgcctgggc
     5641 gttcgcggcc ggggtcttcg cgtgggtggc gggcaacgtc gctgcgttcg cgct
//
LOCUS       DASUEV010000049         9823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_816757, whole genome shotgun sequence.
ACCESSION   DASUEV010000049 DASUEV010000000
VERSION     DASUEV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816757"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /gene="queF"
                     /locus_tag="VFV55_01635"
     CDS             complement(<1..414)
                     /gene="queF"
                     /locus_tag="VFV55_01635"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX4943020.1"
                     /translation="MGSGARETASKDLETFANPHPGRDYVIRMEIPEFTCLCPKTGQP
                     DFATLYLDYVADRKCVELKSLKLYVWSFRDKGRFHEAVANEILDDLVAATAPRFMRLT
                     ARFYVRGGIFTTVVAEHRKPGWQPPPRVDLAGFPAQ"
     gene            600..4121
                     /gene="smc"
                     /locus_tag="VFV55_01640"
     CDS             600..4121
                     /gene="smc"
                     /locus_tag="VFV55_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX4943021.1"
                     /translation="MRLTSLKLSGFKSFVDPTQIAVPGQLVGVVGPNGCGKSNVIDAV
                     RWVLGESSAKQLRGEHMHDVIFNGSTERKPVSRASVELVFDNSLGRVSGSWSQYAEIS
                     VKRVLSRDGDSSYYINGTHVRRRDVQDIFLGTGLGPRAYSIIEQGMISRVITSKPEEL
                     RVFLEEAAGVSKYRERRRETELRLEDTRENLARVGDIRAELDTQLEHLTRQAEVAARY
                     QDLQDQLALNHNLLAFTRLREAEQAKNRCANEVQKVQVQLEAETAKLRDAERALEELR
                     SRHYEETDQLSTLQGALYAANGEVQSLEQEIAFLGENRRRMGAQLEGLATEIAEVERR
                     IAEAQSDAARWKGEIESARAAIGEREAQMQAAKAGLPEAQAAQEKAAAEVRAAEAEMS
                     TAEQAQGVEETREAHALKILAQLEGRKNRLKQENMALVFPEPEKLAAIAAQREQTGVK
                     VVALEEAQRAIEAKLPALEEERQQSQQVHQDRTREAAQLEAALKALEAQQAKLDNNSR
                     LADWVKAHALDRAERLWQAIRVEAGWDDAVEAALGVRLNAARLADEGGMAELLRDAPP
                     GSFAVFVERGAPEAAAPGSNLRPLSSVVSSARTGVAAYLKDALANVFILPEGEDGLAL
                     ARILPPGGLLVAKDGHLFSRQGVVFHGPQSELHGVLQRQREIEELQAKAPSVAAARNA
                     VEARLRELESLLAQSRDEARRLREELSRTRQADHALEVEHLKLSQSSEQAERRREAIR
                     AELAEIEREEGKERLEMQEAQHALESGSQKIEQVVQRLQGLEAGAKQAGEALNAARAA
                     VMAAERAVQEAKFHERSCHEKVESQGTLAVSLGERLAALAANRANVSAELGKLEEGTV
                     RERLQAALAVKAERERELADARAGLEHLTDELRALEEGRLAAEQTLAPLRERITELRL
                     KEQEAATHAEQYAQQIQEAGVTREELAEKLEKRVRSSGMQAEITRLTEEIAALGPVNL
                     AALSELATARERKTFLDAQAADLTQAVETLEAAIKRIDRETRELLQGTFDTVNRNFQE
                     MFPALFGGGNASLKLTGEEILDAGLQVFAQPPGKKNASIQLLSGGEKALTAIALVFSL
                     FRLNPAPFCLLDEVDAPLDDPNTERFCDLVKQMSAQTQFLFITHNKITMEMGQQLIGV
                     TMQEPGVSRIVEVDIGAAMEFARKQAA"
     gene            4132..5217
                     /locus_tag="VFV55_01645"
     CDS             4132..5217
                     /locus_tag="VFV55_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX4943022.1"
                     /translation="MSDLQLALIALGAALVAAVAGYNALSERRARRRAETAFRSSHPD
                     ALLDPEEQRREPVLGVLPGDARQDPSLGALDEVIDPVGGGEPAQAAAASPSAVISGRV
                     DAIALVLADEPVTREQLEPLLDALEAHATPVHVEGLVDEQWMPVEDSPRDRWRELRAG
                     LQLASRAGPVAEEEIAAFNETVAGFAASINAVSQRESPAAAAARSRELDRFCAEADIE
                     VAVNLVGRAGATFAIARVKSLALESGFAETASGSFERRGADGAAAMTLRLADSEPRRD
                     SVYVTGLSFALDVPHVADPVAVLDEMGKVAEAFGLALGGEMVDDERRPLGAAGFAAIR
                     RSLEAVARRMEAHGIPPGGALARRLFS"
     gene            5220..7238
                     /gene="ligA"
                     /locus_tag="VFV55_01650"
     CDS             5220..7238
                     /gene="ligA"
                     /locus_tag="VFV55_01650"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX4943023.1"
                     /translation="MAAPRAVAERVLGLRREIERHNHLYYVLDAPEISDAEWDAMFAE
                     LQALEAAHPELVSPDSPTQRVGARPAEGFAEVTHRVAMLSLANAFTGEDVAAFDRRCR
                     EGLGVQSVEYSCELKFDGLAVTLAYEDGVFVRGATRGDGETGEDVTANLRTVRAIPLR
                     LRSAKPPRLLDVRGEVLMLRRDFEAYNARAQEAGEKLLVNPRNGAAGGLRQLDPRLTA
                     KRRLSFFAYGIGAHEGWDVPSTHAGLLDALAELGFPIAKDHRTAQGEEGLLAFYAEIG
                     TRRERLPYDIDGVVYKVNARADQEALGFVSRAPRWALAHKFPAEEAVTELLDIGVQVG
                     RTGALTPVARLKPVFVGGTTVTNATLHNEDEVRRKDVWRRDTVVVRRAGDVIPEVARV
                     AKPGPREKSDWFSMPAACPECQSAIARLEGEAIARCTGGLICPAQRKASLLHFASRRA
                     MDIEGLGDKLVDQLVDAGLVRTPADLYRLDLAKLAALERMAEKSAANVLAAIERSKET
                     TLGRFVFALGIRHVGEVTAKDLARHFGGLDALMAADSGALEQARDVGPVVAESAAGFF
                     AEPHNREVIAELRAAGVRWTEGEPARAATAGPFAGKIVVLTGTLSGMTRDEAKERVEA
                     AGGRVAGSVSKKTDYVVAGAEAGSKLERAHELGVEVLDEARFRAMLEG"
     gene            7247..8131
                     /gene="galU"
                     /locus_tag="VFV55_01655"
     CDS             7247..8131
                     /gene="galU"
                     /locus_tag="VFV55_01655"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX4943024.1"
                     /translation="MKKIRKAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVAAGVTDLIFVTGRGKRAIEDHFDKAYELEAELRVHGKSRLLRELPGLPEGVTCVYV
                     RQGEALGLGHAVLCARHLVGDEPFAVILADDLIDAKVPALKQMLNLHVKKGASLVAVQ
                     KVDRADTGSYGIVSTKAPKGRLSRVTGIVEKPKPQEAPSTLGVVGRYVLSPRIFDYLV
                     ETRAGAGREIQLTDAIGRLLVDEPVFAYEFRGRRYDCGSKLGYLEATVDYALQHAEVG
                     AGFRAYLDKLAGKGGKRP"
     gene            8128..8670
                     /locus_tag="VFV55_01660"
     CDS             8128..8670
                     /locus_tag="VFV55_01660"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEX4943025.1"
                     /translation="MNRDEARDLLERADLVCPADDVRAAVDRLAAEITARLKDEFPMV
                     LSIMGGAVIFTGQLLPLLAFPLEFGAIEVTRYGNTTEGRNIDWRLAPKDDVRGRTVLV
                     IDDILDEGVTLAAVRDRLLAMGAKRVLAAVFADKDLGRDKPIAADFVGVRVPDRYVFG
                     FGMDAYGLWRNLPAVYALGT"
     gene            8673..9422
                     /locus_tag="VFV55_01665"
     CDS             8673..9422
                     /locus_tag="VFV55_01665"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX4943026.1"
                     /translation="MLAIIGGSGLTGLASLEVTRKQIVRTPYGEPSGPLTLGRIHGGE
                     VAFLARHGYGHTLAPHEINYRANLWALKDRGITRILAVCTVGGIAPGLAAGDIVIPDQ
                     IIDYTWGRDSTFFEGAEQPVVHVDFTHPFCETTRGMLIDAAAAAGVAVVPRGTYAAVQ
                     GPRLETAAEVNRLERDGADFVGMTGMPEAALARELEIPYATLAVVANPAAGRGSSVQA
                     ISLEDIAKVLKEAMGRVRRVVETVVQRHGHP"
     gene            9409..>9823
                     /locus_tag="VFV55_01670"
     CDS             9409..>9823
                     /locus_tag="VFV55_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX4943027.1"
                     /translation="MAIREVLRMGHPVLRQVARPVERFGTAELGRLLEDMKDTMAHED
                     GAGLAAPQIGESVRVVIFGVASNPRYPDAEEVPFTVLVNPVIHFLTEEMEEGWEGCLS
                     VPGLRGMVPRRTKIRYTGFDERGRPIDRIAEGFHAR"
BASE COUNT         1562 a         3366 c         3578 g         1317 t
ORIGIN      
        1 ttgtgccgga aagcccgcca ggtccacgcg cggcggcggc tgccagcccg gcttgcggtg
       61 ctcggcgacc acggtggtga agatgccgcc gcgcacgtag aacctcgcgg tgagccgcat
      121 gaagcgcggg gcggtcgccg ccaccaggtc gtcgaggatc tcgttggcga cggcctcgtg
      181 gaaccggccc ttgtcccgga acgaccagac gtagagcttg aggctcttca gctccacgca
      241 cttgcggtcg gcgacgtaat cgagatacag cgtcgcgaag tccggctggc cggtcttcgg
      301 gcacaggcac gtaaactccg ggatctccat gcggatcacg tagtcgcgcc cgggatgcgg
      361 attggcgaag gtctcgagat ccttcgaggc cgtttcgcgc gctccgctgc ccagcgccct
      421 caagcgcgaa attcccgttt cggcgatctc gcgggcagcg gctgtcaagt tctttttgct
      481 cggctttttc gaaggcattg ggggcgagga ggcgatcgcg gtaaaatcgc gattgtacgc
      541 gaaccccaag ccccaccggc cgcccggcca ggtggggttt tctcataaga atcactatct
      601 tgcgacttac ctccctcaag ctctccggct tcaagtcctt cgtcgacccg acccagatcg
      661 ccgttcccgg ccagctggtg ggcgtggtcg gtcccaacgg ttgcggcaag tccaacgtga
      721 tcgacgcggt gcgctgggtg ctcggggagt cgagcgccaa gcagctgcgc ggcgagcaca
      781 tgcacgacgt gatcttcaac ggctcgaccg agcgcaagcc cgtgagccgc gcctccgtgg
      841 agctcgtgtt cgacaactcg ctgggacgcg tgagcggctc gtggtcgcag tacgccgaga
      901 tcagcgtgaa gcgcgtgctc tcgcgcgacg gcgactccag ctattacatc aacggcacgc
      961 acgtgcgccg ccgcgacgtc caggacatct tcctcggcac cggcctcggc ccgcgcgcct
     1021 actcgatcat cgagcaggga atgatctcgc gcgtgatcac ctcgaagccg gaggagctgc
     1081 gcgtcttcct cgaggaggcc gccggggtat ccaagtaccg cgagcgccgc cgcgagaccg
     1141 agcttcgcct cgaggacaca cgggagaatc tcgcacgcgt aggcgacatc cgcgccgagc
     1201 tcgacacgca gctcgagcac ctcacccggc aggccgaggt ggccgcgcgc taccaggacc
     1261 tccaggacca gctcgccctc aatcacaacc tgctcgcctt cacgcggctg cgcgaggcgg
     1321 agcaggcgaa gaaccgctgc gccaatgagg tgcagaaggt gcaggtgcaa ctggaggcgg
     1381 agacggcgaa gctgcgcgat gccgagcgcg cgctggagga gctgcgcagc cgccactacg
     1441 aggagacgga ccagctctcg acgctccagg gtgcgctcta cgccgccaat ggcgaagtgc
     1501 agtccctcga gcaggagatc gccttcctcg gcgagaaccg ccggcgcatg ggcgcgcaac
     1561 tcgagggcct ggccaccgag atcgcagagg tggagaggcg catcgcggag gcgcagtccg
     1621 acgctgcgcg ctggaaaggc gagatcgaaa gcgcccgcgc ggccatcggc gagcgcgaag
     1681 cgcagatgca ggcggcgaag gcgggcctgc cggaggcgca ggccgcccag gaaaaggcgg
     1741 cggcggaagt gcgcgccgcc gaagccgaga tgtccaccgc ggagcaggcg caaggcgtcg
     1801 aggagacgcg cgaagcgcac gcgctcaaga tcctcgccca gctcgagggg cgcaagaacc
     1861 gcctcaagca ggagaacatg gcgctggtgt tccccgagcc ggagaagctt gcggccatcg
     1921 cggcccagcg cgagcagacc ggcgtgaagg tggtggcgct cgaggaggcg cagcgcgcca
     1981 tcgaggcgaa gctcccggcc ctcgaggagg agcgccagca gtcgcagcag gtgcaccagg
     2041 acaggacgcg cgaggccgcg cagctcgagg cggcattgaa ggctctcgag gcgcagcagg
     2101 ccaagctcga caacaactcg cggcttgccg actgggtgaa ggcgcacgcg ctggaccgcg
     2161 cggagcgcct gtggcaggcg atacgcgtcg aggcgggctg ggacgatgcc gtggaggcgg
     2221 cgctgggcgt gcgcctgaac gccgcgcggc tcgccgacga gggcggcatg gccgagctct
     2281 tgcgcgacgc gcccccgggc agcttcgcgg tgttcgtgga acgcggggcg ccggaagcgg
     2341 ccgcgcccgg atcgaacctg cggccccttt cctccgtcgt ttcgagcgcg cgaacaggtg
     2401 tcgcggccta cctgaaggac gcactcgcca acgtgttcat cctgcccgag ggcgaggacg
     2461 gcctggcgct cgcgcgcatc ctgccgcccg gcgggctgct ggtcgcgaag gacggtcacc
     2521 tgttcagccg ccagggcgtg gtcttccacg gaccccagtc ggaactgcat ggcgtgctgc
     2581 agcgccagcg cgagatcgag gagttgcagg cgaaggcgcc ttccgtcgcg gccgcgcgca
     2641 acgccgtcga ggcgcggctg cgcgagctgg agtcgctcct ggcgcagtcc cgggacgagg
     2701 cgcgccggct gcgcgaggaa ctctcgcgca cgcggcaggc cgaccacgcg ctcgaggtcg
     2761 agcacctgaa gctctcgcag agctccgagc aggcagaaag gcggcgcgag gccattcgcg
     2821 ccgagctcgc cgaaatcgag cgcgaggaag gcaaggagcg cctcgagatg caggaggcgc
     2881 agcacgccct cgaatcgggc tcgcagaaga tcgagcaggt cgtgcagcgc ctgcaggggc
     2941 tggaagccgg cgcgaagcag gcgggcgagg cgctgaacgc cgcgcgcgcc gcggtcatgg
     3001 cggccgagcg cgcggtgcag gaggcgaagt tccacgagcg cagctgccac gagaaggtcg
     3061 agtcgcaggg aacgctcgcc gtttccctgg gcgagcgcct cgcggcgctt gccgccaacc
     3121 gcgccaacgt cagcgcggaa ctgggcaagc tcgaggaagg cacggtgcgc gagcggctgc
     3181 aggccgcgct cgccgtgaag gcggagcgcg agcgcgaact cgcggacgcg cgcgcaggcc
     3241 tggagcacct gacggacgag ttgcgcgccc tcgaggaggg tcgcctcgcc gcggaacaga
     3301 cgctcgctcc cctgcgcgag cgcatcaccg agcttcggct caaggagcag gaagcggcca
     3361 cccacgccga gcagtacgcg cagcagatcc aggaggccgg cgtcacccgc gaggaactgg
     3421 ccgagaagct cgagaagcgc gtgcgctcct ccggcatgca ggcggagatc acgcgattga
     3481 cggaagagat cgcggcgctg ggaccggtga acctcgccgc actctccgag ctcgcgacgg
     3541 cccgcgaacg caagaccttc ctcgacgcgc aggccgccga cctcacgcag gccgtggaaa
     3601 cgctggaggc ggccatcaag cgcatcgacc gcgagacgcg cgagctcctg cagggaacct
     3661 tcgacacggt caacaggaac ttccaggaga tgttcccggc gctcttcggc ggcggcaatg
     3721 cctctctcaa gctcaccggc gaggagatcc tggatgccgg cctgcaagtc ttcgcgcagc
     3781 cgccgggcaa gaagaacgcc tcgatccagc tgctctccgg tggcgagaag gcgctgaccg
     3841 ccatcgcgct cgtcttctcg ctcttccgcc tgaacccggc gcccttctgc ctgctggacg
     3901 aggtggacgc gcccctggac gaccccaaca ccgagcgatt ctgcgacctg gtgaaacaga
     3961 tgtcggcgca gacccagttc ctcttcatca cgcacaacaa gatcaccatg gagatgggcc
     4021 agcagctcat cggcgtgacg atgcaggagc ccggcgtctc gcgtatcgtg gaagtcgaca
     4081 tcggagccgc gatggaattc gcccgcaaac aggcagctta ggagccgatc catgagcgac
     4141 ctgcagctcg ccctcatcgc cctcggcgcc gccctggtgg ctgcggtggc gggctacaac
     4201 gcgttgagcg agcgccgcgc gcgtcgccgc gccgaaaccg cctttcgcag cagccatccc
     4261 gatgcgctcc tcgaccccga ggagcagcgc cgggagcccg tgctgggcgt gctgcccggg
     4321 gatgcgcggc aggatccgtc gctcggcgcc ctcgacgagg tgatcgatcc cgtgggcggc
     4381 ggggaacccg cgcaggccgc ggccgcgtcg ccgtcggccg tcatttccgg ccgcgtcgat
     4441 gccatcgcgc tggtgctggc cgacgagccg gtcacgcgcg agcagctcga gccgctcctc
     4501 gacgcgctgg aggcgcacgc taccccggtc cacgtggagg gactcgtcga cgagcagtgg
     4561 atgcccgtcg aggattcgcc gcgcgaccgg tggcgggagt tgcgcgcggg actgcagctc
     4621 gccagccgcg ccggccccgt ggccgaggag gagatcgccg cgttcaacga gacggtggcg
     4681 ggcttcgcgg cctcgatcaa cgcggtgtcg cagcgcgaga gcccggccgc ggccgcggcg
     4741 cgctcgcgcg agctcgaccg cttctgcgcc gaggccgaca tcgaggtcgc ggtgaacctc
     4801 gtcggccgcg ccggcgccac ctttgccatc gcgcgcgtga aatcgctcgc gctcgaaagc
     4861 ggcttcgcgg agacggcatc gggcagcttc gagcgccgcg gggcggacgg ggccgcggca
     4921 atgacgctgc gcctggcgga cagcgagccg cggcgcgaca gcgtctacgt gaccggcctt
     4981 agcttcgcgc tggacgtccc gcacgtcgcg gacccggtcg cggtgctcga cgagatgggc
     5041 aaggtcgcgg aggcgttcgg cctcgcgctc ggcggggaga tggtcgacga cgaacgccgg
     5101 cccctgggcg cggccggctt cgccgcgatc cgccgctcgc tggaggcggt ggcgcggcgc
     5161 atggaggcgc acggcattcc gccgggcgga gcgctcgcgc ggcggctctt ctcctgaaga
     5221 tggcggcgcc gcgcgcggtc gcggagcggg tcctcgggct gcggcgcgag atcgagcggc
     5281 acaatcatct ctattacgtc ctcgacgcgc cggaaatctc cgacgccgag tgggacgcga
     5341 tgttcgccga attgcaggca ctcgaggccg cgcacccgga gctcgtgtct cccgactcgc
     5401 ccacgcagcg cgtcggcgca aggcccgcgg agggtttcgc ggaggtcacg caccgcgtcg
     5461 ccatgctctc gctcgccaat gccttcaccg gcgaggacgt ggcggcgttc gaccggcgct
     5521 gccgcgaggg actcggggtg cagagcgtcg aatactcgtg cgagctcaag ttcgacggcc
     5581 tcgcggtgac gctcgcctac gaggacggcg tgttcgtccg cggcgccacg cgcggcgacg
     5641 gcgagacggg cgaggacgtg acggccaacc tgcgcacggt ccgcgcgatt ccgctgcggc
     5701 tgcgctcggc gaagccgccg cggctgctgg acgtgcgcgg cgaggtgctg atgctgcggc
     5761 gcgacttcga ggcgtacaac gcccgggcgc aggaggccgg cgagaagctg ctggtcaatc
     5821 cgcgcaacgg cgccgcggga ggcctgcgcc agctcgaccc gcgcctcacc gcgaaacggc
     5881 gcctgtcgtt cttcgcctac ggcatcgggg cgcacgaggg atgggacgtg ccatccacgc
     5941 acgccgggct cctcgacgcg ctggccgaac tgggcttccc gatagcgaag gaccatcgca
     6001 cggcgcaggg cgaagagggg ctgctcgcct tctacgccga gatcggaacc aggcgcgagc
     6061 gattgcccta cgacatcgac ggcgtggtgt acaaggtgaa cgcgcgggcc gaccaggagg
     6121 cgctcggttt cgtgtcgcgc gccccgcgct gggcgctggc gcacaagttc ccggcggaag
     6181 aagcggtgac cgagctgctc gacatcggtg tccaggtggg gcgcaccggc gcgctcacgc
     6241 ccgtggcacg gctcaagccc gtcttcgtcg gcggcacgac ggtcaccaac gcgacgctgc
     6301 acaacgagga cgaggtgcgc cgcaaggacg tgtggcggcg cgacaccgtc gtggtgcggc
     6361 gcgccggcga cgtgatcccg gaagtggcgc gcgtggcgaa gccgggcccg cgcgagaagt
     6421 ccgactggtt ctcgatgccg gcggcgtgcc ccgaatgcca gtcggccatc gcgcggctcg
     6481 agggcgaggc catcgcgcgt tgcaccggcg gcctcatctg ccccgcgcag cgcaaggcgt
     6541 cgctcctgca cttcgcctcg cgccgcgcca tggacatcga ggggctgggc gacaagctgg
     6601 tcgaccagct cgtggatgcc ggcctcgtga ggaccccggc cgacctctac cgcctcgacc
     6661 tcgccaagct cgccgcactc gagcgcatgg ccgagaagtc ggcggccaac gtgctcgccg
     6721 ccatcgagcg gagcaaggag acgacgctcg ggcgcttcgt cttcgcgctg ggcatccgcc
     6781 acgtgggcga ggtcaccgcc aaggatctcg cacgtcattt cggcggcctg gatgcgctga
     6841 tggccgcgga cagcggtgca ctggagcagg cgcgcgacgt gggaccggta gtcgccgaga
     6901 gcgcggcagg cttcttcgcc gagccgcaca atcgcgaggt catcgccgaa ttgcgcgcgg
     6961 ccggcgtgcg ctggaccgag ggcgaacccg cccgcgccgc caccgccgga cccttcgcgg
     7021 gtaaaatcgt cgtgctcacg ggaacgcttt ccggaatgac gcgcgacgag gcgaaggaac
     7081 gcgtcgaggc cgccggcggc agggtcgccg ggtcggtgtc gaagaagacc gattacgtcg
     7141 tcgcgggagc ggaagcgggc tccaagctcg agcgcgcgca cgagctgggc gtcgaggtgc
     7201 tggacgaggc gcggtttcgc gccatgctcg aaggctgaag aagaccatga agaagatccg
     7261 caaggccgta ttccccgtcg cgggcctggg cacgcgcttc ctgcccgcca ccaaggcctc
     7321 gccgaaggag atgctcccca tcgtcgacaa gccgctcatc cagtatgcgg tggaggaggc
     7381 cgtggccgcg ggcgtgaccg acctgatctt cgtcaccggc cgcggcaagc gcgccatcga
     7441 ggatcatttc gacaaggcct acgagctcga ggcggaactg cgcgtccacg gcaagagccg
     7501 gctcctcagg gaattgccgg ggctgccgga gggcgtgacc tgcgtctacg tccggcaagg
     7561 cgaagcgctc ggtctcggcc acgccgtgct gtgcgcgcgc catctcgtgg gcgacgagcc
     7621 cttcgccgtg atcctcgccg acgacctcat cgacgcgaag gtgccggcgc tcaagcagat
     7681 gctcaacctg cacgtgaaga agggcgcgag cctggtcgcg gtgcagaagg tcgatcgcgc
     7741 cgataccggc tcctacggca tcgtctcgac gaaagcgccc aagggccgcc tttcgcgcgt
     7801 caccggcatc gtggaaaagc cgaagccgca ggaggcgccg tcgaccctgg gcgtggtcgg
     7861 acgctacgtc ctcagcccgc gcatcttcga ttacctggtc gaaacgcgcg cgggcgcggg
     7921 tcgagagatc cagctcaccg acgcgatcgg ccgcctgctc gtggacgaac ccgtgttcgc
     7981 ctatgaattc cgcggccggc gctacgattg cggctccaag ctcggctacc tcgaggccac
     8041 ggtggactac gccctgcagc acgccgaggt gggtgccggg ttccgcgcct atctcgacaa
     8101 gctcgccggc aagggcggga agcggccatg aaccgcgacg aggcccggga cctgctcgag
     8161 cgcgccgacc tggtgtgccc ggcggacgat gtccgcgccg cggtggaccg gctcgccgcg
     8221 gagatcaccg cgcggctgaa ggacgagttc ccgatggtgc tgtcgatcat gggcggggcg
     8281 gtgatcttca ccgggcagct gctgccgctg ctcgccttcc cgctcgagtt cggcgccatc
     8341 gaggtgacgc gctacgggaa cacgaccgag gggcgcaaca tcgactggcg cctggcgccc
     8401 aaggacgacg tgcgcgggcg aacggtgctg gtgatcgacg acatcctcga cgagggggtc
     8461 acgctcgccg ccgtgcgcga ccggctcctg gcgatgggag cgaagcgcgt gctcgcggcg
     8521 gtgttcgccg acaaggacct cgggcgggac aagcccatcg cggcggattt cgtcggcgtg
     8581 cgcgtgcccg accgctacgt gttcggcttc ggcatggacg cctacggtct gtggcgcaac
     8641 ctccccgcgg tctacgcgct cgggacctag cgatgctggc catcatcggc ggttccggcc
     8701 tcaccggcct cgcgagcctc gaggtcacga ggaagcagat cgtgcgcacg ccctacggcg
     8761 agccctccgg ccccctcacg ctgggccgca tccacggcgg cgaggtggcc ttcctcgcgc
     8821 gccacggcta tgggcacacg ctggccccgc acgagatcaa ctaccgggcc aacctctggg
     8881 cgctgaagga caggggcatc acgcgcatcc tcgccgtgtg caccgtgggc ggcatcgcgc
     8941 ccgggctcgc ggccggtgac atcgtgatac ccgaccagat catcgactac acctggggac
     9001 gcgattccac cttcttcgag ggagccgagc agccggtggt gcacgtggac ttcacgcacc
     9061 ccttctgcga gacgacgcgg ggcatgctca tcgatgcggc ggccgcggcc ggcgtggcgg
     9121 tggtgccgcg cggaacctat gctgccgtcc aggggccgcg cctggagacg gccgcggaag
     9181 tgaaccggct cgagcgcgac ggcgccgatt tcgtgggaat gaccggcatg ccggaggcgg
     9241 cgctggcccg cgagctcgag atcccgtacg cgaccctggc ggtggtggcc aacccggccg
     9301 ccgggcgcgg ctcgagcgtg caggcgatct cgctcgagga catcgcgaag gtgctgaagg
     9361 aagccatggg gcgcgtgcgg cgcgtggtcg agacggtggt gcagcgccat ggccatccgt
     9421 gaggtgctgc gcatgggcca cccggtgctg cgccaggtcg cgcggcccgt ggagcgcttc
     9481 ggcacggcgg agctcgggcg cctgctcgag gacatgaagg acacgatggc ccacgaggac
     9541 ggcgcgggcc tcgcggcgcc gcagatcggc gagagcgttc gcgtggtgat cttcggcgtc
     9601 gcctcgaacc cgcgctaccc ggacgcggag gaggtaccct tcaccgtgct cgtgaacccc
     9661 gtcatccact tcctcaccga ggagatggag gaaggctggg aggggtgcct gtccgtgccg
     9721 ggcctgaggg gcatggtgcc gcggcgcacg aagattcgct acaccggctt cgacgagcgc
     9781 ggccggccca tcgaccgcat cgcggagggg ttccacgcgc gcg
//
LOCUS       DASUEV010000050         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_931671, whole genome shotgun sequence.
ACCESSION   DASUEV010000050 DASUEV010000000
VERSION     DASUEV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931671"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..370
                     /locus_tag="VFV55_01675"
     CDS             143..370
                     /locus_tag="VFV55_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX4943028.1"
                     /translation="MEYTKELDARGLNCPLPILKTKKALGDLTTGQVLKVISTDAGSV
                     KDMEAFAKQTGNELLASEPQGKEFVFFLKKK"
     gene            381..851
                     /locus_tag="VFV55_01680"
     CDS             381..851
                     /locus_tag="VFV55_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX4943029.1"
                     /translation="MSGKKLAIIATKGTLDMAYPPFILASTAAALDYEVQIFFTFYGL
                     TLLRKDLSGVKISPLANPAMPMPMPMPVLVAALPGMESMATMMMKQKIKSKGVASIEE
                     LRSACIEGGVKFIGCEMTVDLFDFKREDFIDGIEYGGASTFFEFAGNTDICLFI"
     gene            899..2188
                     /locus_tag="VFV55_01685"
     CDS             899..2188
                     /locus_tag="VFV55_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4943030.1"
                     /translation="MKTIPTAVRRALATTACLLPLAAFATDGYFSHGYGMKAKGMAGA
                     ATAMAADSMGGANNPASMVWVGNRFDVGVDLFSPRRKVSREGSGGGVLDFSVDSDSTL
                     FAIPELGYNMMFSPNVSLGVTVYGNGGMNTDFEGGQLNCSPFGGPPSANGLCGVGRLG
                     VNLEQLIIAPTVAWKFAPDHSLGVSPLFGYQKFRMQGAHLFTQLSQSPGEVTNRDHDN
                     ATGFGVRVGYMGRIGPMVTVGATYASKLKMSEFDKYKGLFAENGDFDLPEHYTVGLAL
                     RPAKDVLVALDYKKIKYSGIASVGNPSTNQAPLGSSGGPGFGWQDVDVFKVGVEFQTS
                     PKLTLRAGYGHTDNPIQARDVTFNILAPGVVQDHYTLGFTYAIDKSSEITGAYMHAAR
                     NSVSGPSFFNAFAPGQGGTETIEMYQNSLGIAWGMRW"
BASE COUNT          450 a          783 c          765 g          401 t
ORIGIN      
        1 agacattacc catgtggggg gtgggggatt acccttagcg ggtatagacc cgcaccaccc
       61 cgggccgcga aattgcggta cccgaacgga cgggcggggc gcggtgcgcc ttgcctcccg
      121 aggacactgc aaggagaacg caatggagta caccaaggaa ctggacgcgc gcggcctcaa
      181 ctgcccgctt ccgatactca agacgaagaa ggccctcggc gatctcacca ccggacaggt
      241 gctgaaggtc atctcgacgg atgcgggctc cgttaaggac atggaagcgt tcgccaagca
      301 gacgggcaac gaactgctcg cctccgagcc gcagggcaag gagttcgtgt tcttcctgaa
      361 gaaaaagtag gagcaagccc atgagcggca agaaactggc gataatcgcc accaagggga
      421 cgctggacat ggcgtacccg cccttcatcc tcgcctccac ggcggccgct ctcgactacg
      481 aggtgcagat cttcttcacc ttctacggcc tgacgctgct gcgcaaggat ctttccgggg
      541 tgaagatcag cccgctcgcc aatccggcca tgccgatgcc tatgccgatg ccggtgctcg
      601 tcgcagcact tcccggcatg gagtcgatgg ccacgatgat gatgaagcag aagatcaagt
      661 cgaagggcgt ggcttcgatc gaggaactgc gcagcgcgtg catcgagggg ggggtcaagt
      721 tcatcggctg cgagatgacc gtcgacctct tcgacttcaa gcgcgaggac ttcatcgacg
      781 gcatcgaata cggcggagcg tccaccttct tcgaattcgc cggcaacacc gacatctgcc
      841 ttttcatctg acattgcggt tccaagctcg ctcgtccact accggaggat tcaagacaat
      901 gaaaacgatc ccgaccgcgg ttcgccgcgc actggccacg acggcgtgcc tgctgccgct
      961 cgccgccttc gcgaccgacg gttacttctc gcacggctac ggcatgaagg ccaagggcat
     1021 ggcgggtgct gctaccgcga tggccgcgga ttccatgggt ggcgccaaca accccgccag
     1081 catggtctgg gtcggcaacc gcttcgacgt tggcgtggat ctcttcagcc cgcgccgcaa
     1141 ggtcagtcgc gagggaagcg gcggcggcgt cctcgacttc tcggtcgaca gcgacagcac
     1201 gctcttcgcc atccccgagc tcggctacaa catgatgttc tcccccaacg tctcgctggg
     1261 cgtgacggtc tacggcaacg gcgggatgaa caccgacttc gagggcggcc agctcaactg
     1321 ctcgccattc ggcggaccgc cgtcggccaa cggcctttgc ggcgtgggcc ggctgggggt
     1381 gaacctggag cagctcatca tcgcccccac cgtggcctgg aagttcgcgc cggaccattc
     1441 gctgggcgtc tcgccgctgt tcggctacca gaaattccgc atgcagggcg cacacctctt
     1501 cacgcagctc tcgcagtcgc cgggcgaagt caccaaccgc gatcacgaca atgcgacggg
     1561 tttcggcgtg cgcgtgggct acatggggcg catcggcccg atggtgacgg tgggtgccac
     1621 ctacgcgtcg aagctcaaga tgagcgagtt cgacaagtac aagggcctct tcgccgaaaa
     1681 cggcgacttc gacctgcccg agcactacac ggtgggtctg gcgttgcggc ccgcgaagga
     1741 cgtgctggtc gccctcgact acaagaagat caagtacagc ggcatcgcct ccgtgggcaa
     1801 tcccagcacg aaccaggcgc cgctgggctc gtcgggcgga ccgggattcg gctggcagga
     1861 cgtggacgtc ttcaaggtgg gcgtcgagtt ccagaccagc ccgaaactca cgctgcgcgc
     1921 ggggtacggc cacacggaca accccatcca ggcgcgtgac gtgaccttca acatcctcgc
     1981 cccgggcgtg gtccaggacc actacacgct gggcttcacg tacgccatcg acaagtcctc
     2041 ggaaatcacc ggcgcctaca tgcacgccgc gcgcaactcg gtgagcggcc cgtcgttctt
     2101 caatgccttc gcaccgggcc agggcggtac cgagaccatc gagatgtacc agaactccct
     2161 gggcatcgcc tggggcatgc gctggtaagc cggacccggg ccgcgccgtt cgcgcgcggc
     2221 ccgctcaccg ccaccgtgcg ggcggtgtcg gccgcgtgcc gaacgttcag cgccaccctg
     2281 cccacggtgt cagcgtccag gggaccttcg cacgagacag tcgccgcgcc gaattggcac
     2341 ggtgatgatc ggagacgtag ggtggggtga ggttggatgg tcccgaaatt tttttgggg
//
LOCUS       DASUEV010000051         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1153492, whole genome shotgun sequence.
ACCESSION   DASUEV010000051 DASUEV010000000
VERSION     DASUEV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153492"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFV55_01690"
     CDS             <1..251
                     /locus_tag="VFV55_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HEX4943031.1"
                     /translation="RKGLSPDTWQDVKLALPGIADPATYEQLKHGFARGHEAMQFVDN
                     VRNYQDILARVEPRDERLAPRPPPPLSQGSTKGSMAAR"
     gene            complement(215..847)
                     /locus_tag="VFV55_01695"
     CDS             complement(215..847)
                     /locus_tag="VFV55_01695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4943032.1"
                     /translation="MSTRLVVVRHGETQWNLDSRIQGYSDSPLTAAGEAQAAAIAERL
                     AGESFDRLVSSDLGRAWRTAQAIAARIGLAVEPDARLRERNYGMAEGLTYGEIGVHYP
                     DLFSRVRDTDPDYVVPGGESRRQLFERVRDAFEALARASDGRCIAVVCHGGVLAALYR
                     HVHGIPVGTAHPIPIPNASYNALVYNCGRWEVEAWADTAHLAAIEPFVEP"
     gene            complement(844..2121)
                     /gene="serS"
                     /locus_tag="VFV55_01700"
     CDS             complement(844..2121)
                     /gene="serS"
                     /locus_tag="VFV55_01700"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX4943033.1"
                     /translation="MLDIQLLRKELPTVVAGLARRGVSFDEAGFRALEDERKGLQTRT
                     EELQAKRNALSKQVGILKGKGEDAAAVLAEVGGIGDELKSNETALAALQERLNGFLRM
                     IPNIPRPEVAAGNSSDDNVEVRRWGTPRTFDFPVKDHVDVGAALGMLDFDTASKLSGA
                     RFSVLKGGVARLHRALAQFMLDVHTREHGYTEVYVPYMVTGECADGVSSLAKFRDDLF
                     KLEGRDLYLIPTAEYPVTNFVRDTLLEAKDLPLRFVCHSPCFRSEAGSYGKDTRGMIR
                     QHQFDKVEILQVVHPDKSAEAHEELTRHAETILQRLELPYRTVMLCTGDLGFSSAKTY
                     DLEVWLPGQQAYREISSCSNFEAFQARRMQARFKGGEKGRAEPVHTLNGSGLAVGRTL
                     VAILENCQQADGSVTVPGALVPYMGGVERIARA"
     gene            complement(2274..2399)
                     /locus_tag="VFV55_01705"
     CDS             complement(2274..2399)
                     /locus_tag="VFV55_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943034.1"
                     /translation="MNTDKPGNSFDVHRVQGEIRDATETMEAMFTDVGARDDGTI"
     gene            complement(2447..>2729)
                     /locus_tag="VFV55_01710"
     CDS             complement(2447..>2729)
                     /locus_tag="VFV55_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HEX4943035.1"
                     /translation="YNAAREFVSRDSSRPVPMHLRNAPTRLMKDLGYGKGYRYAHDEE
                     GGYAAGERYLPDGMDAPGWYQPTDRGLEAKIREKLAWLRQRDKGTGDGE"
BASE COUNT          437 a          916 c          926 g          450 t
ORIGIN      
        1 agaggaaggg gctgtcgccc gacacgtggc aggacgtgaa gctggcgctc ccgggaatcg
       61 cggatccggc gacctacgag cagctcaagc acgggttcgc gcgcggccac gaggcgatgc
      121 aattcgtgga caacgtgcgc aattaccagg acatcctcgc ccgcgtggag cctcgcgatg
      181 aacggctcgc gccccggccg ccgcctcccc tgtctcaggg ctccacgaaa ggctcgatgg
      241 ccgcgaggtg agcggtgtcg gcccacgcct ccacttccca gcgcccgcag ttgtacacga
      301 gcgcgttgta ggaggcattg gggatcggga taggatgcgc agtcccgacg gggatcccgt
      361 ggacatgacg gtagagcgcg gcgagaacgc cgccatggca gaccacggca atgcaccggc
      421 catcggaagc gcgcgccagc gcctcgaagg cgtcgcgcac gcgctcgaaa agctgccgcc
      481 ggctctcgcc gccaggcacc acgtagtccg ggtcggtgtc gcgcacgcgc gagaaaaggt
      541 cggggtaatg cacgccgatc tcgccgtagg taagtccctc ggccattccg tagttgcgct
      601 cgcgcagccg cgcatcgggc tcgaccgcga ggccgatgcg cgccgcgatg gcctgcgccg
      661 tgcgccacgc gcggccgagg tcgctcgaga cgagccggtc gaacgactct cccgcgaggc
      721 gctcggcgat cgccgcggcc tgcgcctcac ccgcggcggt gagtgggctg tccgagtagc
      781 cctggatgcg cgagtcgagg ttccactgcg tctcgccatg gcggacgacg acgaggcgcg
      841 tgctcaagcg cgcgcgatcc gctcgacgcc gcccatgtac ggcacaagcg cgcccggcac
      901 cgtgaccgag ccatcggcct gctggcagtt ctcgaggatc gccacgagcg tgcgccccac
      961 cgcgagcccc gagccgttga gcgtgtgcac cggctccgcg cggccttttt cgccgccctt
     1021 gaaccgcgcc tgcatgcgac gcgcctggaa ggcctcgaaa ttggagcacg acgagatctc
     1081 ccggtaggcc tgctggcccg gcagccagac ctcgaggtcg taggtcttgg cactggaaaa
     1141 accgaggtcg cccgtgcaca gcatcaccgt ccggtagggc aactccaggc gctgcaggat
     1201 cgtctccgca tgccgcgtga gctcctcgtg cgcctcggcc gacttgtccg ggtgcaccac
     1261 ctgcaggatc tccaccttgt cgaactggtg ctggcggatc atgccgcgcg tgtccttgcc
     1321 gtaactgccg gcctccgagc ggaagcaggg gctgtggcac acgaacctga gcggcaggtc
     1381 cttcgcctcg agcagcgtgt cgcgcacgaa gttcgtgacc gggtactcgg cggtggggat
     1441 gaggtagagg tcgcgcccct cgagcttgaa gaggtcgtcg cggaacttcg ccaggctcga
     1501 gacgccatcc gcgcattcgc ccgtcaccat gtacgggacg tagacctccg tgtaaccgtg
     1561 ctcgcgcgtg tggacgtcga gcatgaattg cgcgagcgcg cggtgcaggc gggcaacgcc
     1621 gcccttgagg accgaaaatc gcgccccgga cagcttggac gcggtgtcga agtcgagcat
     1681 gcccagggct gcgcccacgt cgacgtggtc cttcaccgga aaatcgaagg tccggggcgt
     1741 tccccagcgg cgcacctcga cgttgtcgtc gctcgaattt cccgccgcca cctcggggcg
     1801 cggaatgttg gggatcatgc gcaggaatcc gttgagccgc tcctgaagcg cggccagcgc
     1861 cgtctcgttc gacttgagct cgtccccgat tccgcccacc tccgcgagca ccgcggccgc
     1921 atcctcgccc ttgcccttga ggatgccgac ctgcttcgag agtgcgttgc gcttcgcctg
     1981 caactcctcg gtgcgcgtct gcagcccctt cctctcgtcc tcgagggcgc gaaagccggc
     2041 ttcgtcgaac gaaacgccgc gacgggcaag cccggccacg accgtgggaa gttccttgcg
     2101 cagcagttgg atgtccagca tgggggagcg gaaaggatgg agggggatga ggattataga
     2161 gtctgggaag gcggggggcg cggtcggtgg ggagtgacgc tacccgcgat ggcgagtgac
     2221 gctacccaaa agctaggaca cggatgaaca cggatgaaca cggataattc ttgttagatg
     2281 gtgccgtcat cgcgtgcgcc aacatcggtg aacatcgcct ccatggtttc agtggcatcg
     2341 cgaatctcgc cttggacccg gtgcacatcg aatgaattgc ctggcttatc cgtgttcatc
     2401 cgtgttcatc cgtgtcctag ctcttgggta gcgtcactcg ccatcgtcac tcgccatcgc
     2461 ccgtcccctt gtcgcgctgg cgcagccagg cgagcttctc gcggatcttc gcctcgagcc
     2521 cgcgatcggt gggctggtac cagcccggcg catccatgcc gtcaggcagg tagcgctcgc
     2581 cggccgcgta gccgccttcc tcgtcgtggg cgtaacggta acccttgccg tagcccaggt
     2641 ccttcatgag ccgcgtgggc gcgttgcgca ggtgcatggg gacggggcgg ctcgaatcgc
     2701 gcgagacgaa ctcgcgggcg gcgttgtag
//
LOCUS       DASUEV010000052         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1383692, whole genome shotgun sequence.
ACCESSION   DASUEV010000052 DASUEV010000000
VERSION     DASUEV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383692"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2297)
                     /locus_tag="VFV55_01715"
     CDS             complement(<1..2297)
                     /locus_tag="VFV55_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX4943036.1"
                     /translation="MGAQIAAHLANAGVQPVLFELAAEGRDKNANVRKALDALAKLEP
                     SPLAVKSVVERITAANYDEHLALLADCDLVIEAISERMDWKKALYDKVAPHIAPHAIF
                     ASNTSGLSITELAKVLPEAIRPRFCGIHFFNPPRYMRLVEIIPTASTAPAIVDDLEAF
                     LTTTLGKGVVRARDTPNFVANRVGVFSMLATMHHTLAFKLGFDEVDALTGPAIGRAKS
                     ATFRTADVVGLDTMAHVVKTMHDTLPADPWHPHYQAPAWLAALIAKGALGQKTKAGIF
                     VKMGKEIHVLDLAKQDYRPSAGAIAEEVQAILKERNPAQQFAKLRASQHPQAQFLWSI
                     YRDLFHYCAVHLAEIAHCARDVDLAIRWGFGWKLGPFEIWQAAGWQQVAGWITEDIAA
                     GKTMAKAPLPGWVTDGRKGVHGPDGSFSASDDTMHTRSTLPVYARQLFPELVLGEKTA
                     DRGTTVFEDEGVRLWHQGDGIAIISFKSKMHSLGSEVLAGLNRAIDEAERNYDALVIW
                     HEAPFAVGANLKAALESLKTGKFDEFMAMVALFQQTSQRLKYSLVPTVAAVEGMALGG
                     GCEFVMHAQRVVAALESYIGLVEVGVGLLPAGGGLKELAQRANAWAKGGDAFPELGRL
                     FRQVAMGDVSKSAEQARDMGYLRPSDVVVFHPAELLYVAKQQARAMAESGHRPPLAPA
                     RIPVAGSTGIATLKMMLVNMKEGGFISDHDYEIGSRIATVICGGEVEPGSFVDEKWLL
                     DLERHHFGELAKTEKTQARIEHMLKT"
     gene            complement(2404..2808)
                     /locus_tag="VFV55_01720"
     CDS             complement(2404..2808)
                     /locus_tag="VFV55_01720"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX4943037.1"
                     /translation="MHANLPQGEQPVLRIIPMPADTNAHGTIFGGWVMAQVDLAGSVP
                     AYERAAGPIVTLAVNSFVFKEPVFVGDLVSFYARVTKVGRTSITVDVEVYAQRGRDGR
                     TDAVKVTEAQLTYVAIDAQRRPRPIPGGIDKA"
     gene            complement(2935..3396)
                     /locus_tag="VFV55_01725"
     CDS             complement(2935..3396)
                     /locus_tag="VFV55_01725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4943038.1"
                     /translation="MRTGLASFETQAPDEAEVRSRFEVVRGRGFPYLVAEQEGHVAGF
                     AYASPYRTRPAYRFSLEDSVYVHPDFTGRGIGRALLARLIEQCATIGCRQMVAIIGDS
                     GNTASIGLHSALGFSLVGTFRAIGFKHGRWVDSVLMQRAIGQGDGTLPELP"
     gene            3277..>3592
                     /locus_tag="VFV55_01730"
     CDS             3277..>3592
                     /locus_tag="VFV55_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943039.1"
                     /translation="MALLFRDKVGKTPAAHDLEAAANFRFVRSLRFEGSKASPHVMAV
                     DRGDRGKVGVVRFADLDAHPAPMTLEKTAFPNGNSAHRIHGSRAALISPTFCVHLCSS
                     VVP"
BASE COUNT          559 a         1177 c         1242 g          614 t
ORIGIN      
        1 gtcttgagca tgtgctcgat gcgggcctgc gtcttttccg tcttcgcgag ttccccgaaa
       61 tgatggcgtt cgagatccag cagccacttc tcgtccacga agctgccggg ctcgacctca
      121 ccgccgcaga tcaccgtggc gatgcgcgag ccgatctcgt agtcgtggtc ggaaatgaag
      181 ccgccctcct tcatgttcac cagcatcatc ttgagcgtgg cgatgcccgt cgagcctgcc
      241 acggggatgc gcgcgggcgc gagcggcggg cggtggcccg actcggccat ggcgcgcgcc
      301 tgctgcttgg cgacgtacag gagttccgcc gggtggaaca ccacgacgtc ggaaggcctg
      361 aggtatccca tgtcgcgcgc ctgctccgcg ctcttcgaga cgtcgcccat cgcgacctgc
      421 cggaacaggc gtcccagttc ggggaaggcg tcgccgccct ttgcccaggc gttggcgcgc
      481 tgcgcgagct ccttgaggcc gccgccggcg ggcagcaggc ccacgcccac ctcgacgagg
      541 ccgatgtagc tttccagcgc cgcgaccacg cgctgcgcgt gcatcacgaa ctcgcagccg
      601 ccgccgagcg ccatgccctc cacggcggcg accgtgggca ccaggctgta cttgaggcgc
      661 tgcgaggtct gctggaagag cgccaccatc gccatgaact cgtcgaactt gccggtcttc
      721 agcgattcca gcgccgcctt caggttggcg cccacggcga agggcgcctc gtgccagatg
      781 acgagggcat cgtagttcct ttccgcctcg tcgatggcgc ggttcaggcc cgcgagcacc
      841 tccgagccca gcgagtgcat cttgctcttg aaggagatga tggcgatgcc gtcgccctgg
      901 tgccacaggc gcacgccctc gtcctcgaag acggtggtgc cgcgatcggc cgtcttctcg
      961 cccagcacca gctcggggaa gagttgccgc gcgtacacgg gcagcgtcga gcgcgtgtgc
     1021 atcgtgtcgt cggaggcgga gaacgagccg tcgggcccgt ggacgccctt gcgcccgtcc
     1081 gtgacccagc ccggcagcgg cgccttggcc atcgtcttgc cggccgcgat gtcctcggtg
     1141 atccaccccg ccacctgttg ccaacctgcg gcctgccaga tctcgaaggg gccgagcttc
     1201 cagccgaatc cccagcggat ggcaaggtcg acgtcgcgcg cgcaatgcgc gatctcggcg
     1261 aggtgcaccg cgcagtaatg gaaaaggtcg cggtagatgg accacaggaa ctgcgcctgc
     1321 ggatgctgcg acgcgcgcag cttcgcgaat tgctgcgcgg ggttcctctc cttgaggatc
     1381 gcctgcacct cctcggcgat ggcgccggcg gaggggcggt aatcctgctt cgcgagatcc
     1441 agcacatgga tctccttgcc catcttcacg aagatgccgg ctttcgtctt ctggccgagc
     1501 gcgcccttcg cgatcaacgc cgccagccac gccggcgcct ggtagtgcgg gtgccacggg
     1561 tcggcgggca gcgtgtcgtg catcgtcttc accacgtgcg ccatggtgtc gaggcccacg
     1621 acgtcggcgg tgcggaaggt ggcgctcttg gcgcgcccga tcgcgggtcc cgtgagcgcg
     1681 tccacctcgt caaagccgag cttgaaggcg agcgtgtggt gcatcgtcgc gagcatcgag
     1741 aacacgccca cgcggttggc cacgaagttg ggcgtgtcgc gggcacgcac cacgcccttg
     1801 ccgagcgtgg tggtgaggaa ggcctcgagg tcgtccacga tggccggcgc ggtcgaagcc
     1861 gtcgggatga tctcgaccag ccgcatgtag cgcggcgggt tgaagaagtg gatgccgcag
     1921 aagcgcgggc ggatggcttc cggcaggacc ttcgcgagtt cggtgatgga aagtcccgac
     1981 gtgttggaag cgaagatcgc gtgcggggcg atatgcggcg ccaccttgtc gtacagcgcc
     2041 ttcttccagt ccatgcgctc gctgatggct tcgatgacga ggtcacagtc agcgaggagc
     2101 gccaggtgct cgtcgtagtt ggcggccgtg atgcgctcga cgaccgattt caccgcgagc
     2161 gggctgggct cgagcttggc cagcgcgtcg agggccttgc gcacgttggc gttcttgtcg
     2221 cggccttccg cggcgagctc gaagaggacc ggctgcacgc cggcattggc gaggtgggcc
     2281 gcgatctgcg cacccatgac accggcgcct aggacagcgg ccttgcggac gaggaatttc
     2341 gacacggtga gtctcctggg acgggcccgc cgcggcgggc tggtattgga agaggtgggg
     2401 cgatcaggcc ttgtcgattc cgcccggaat cgggcgcggg cggcgctggg cgtcgatcgc
     2461 cacataggtg agttgcgcct cggtgacctt tacggcatcc gtccgcccgt cgcggccccg
     2521 ctgggcgtac acctccacgt ccacggtgat cgaggtccgg ccgaccttgg tcacgcgggc
     2581 gtagaagctc acgaggtcgc cgacgaacac cggctccttg aagacgaacg agttgacggc
     2641 cagcgtcacg atcggccccg cggcgcgctc gtaggcgggg acgctgcccg caaggtcgac
     2701 ctgcgccatc acccatcccc cgaagatggt gccgtgcgcg ttcgtatccg ccggcatggg
     2761 gatgatgcga aggacgggct gttcgccctg cggaagattg gcgtgcatgg gggtttcaaa
     2821 cgggcgtttg attctacacc ggtggcaatc gagtcgtggt cgccgatgcg gacgtagggc
     2881 ggcttccgga caggcagggg tcgaggtctc ggctcgccaa ggggtagagc ctgtctacgg
     2941 aagctcggga agcgtgccgt cgccctggcc gatggcgcgc tgcatcagca cgctatcgac
     3001 ccaccgcccg tgcttgaagc cgatcgcacg gaaggtgccg accagggaaa agcccagcgc
     3061 cgaatgcagg ccgatggaag cggtattgcc gctgtcgccg atgatcgcga ccatttgccg
     3121 gcagccgatt gtcgcgcatt gctcgatgag ccgcgcgagg agcgcgcgcc cgatgccccg
     3181 gcccgtgaaa tccgggtgga cgtagacgga gtcctcgagg ctgaagcgat aggccgggcg
     3241 ggtccggtag gggctcgcat aggcaaagcc ggccacgtgg ccctcctgtt ccgcgacaag
     3301 gtagggaaaa cccctgccgc gcacgacctc gaagcggctg cgaacttccg cttcgtcagg
     3361 agcctgcgtt tcgaaggaag caaggccagt ccgcacgtga tggccgtaga tcgcggtgat
     3421 cgcggcaagg tcggcgtcgt gcgcttcgcg gacctcgacg ctcatccggc accgatgact
     3481 ctcgaaaaga ccgcttttcc gaacggcaat tccgcgcatc gcatccacgg atccagggcc
     3541 gcactcattt caccgacctt ctgtgttcat ctgtgttcat ccgtggttcc aa
//
LOCUS       DASUEV010000053         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_130888, whole genome shotgun sequence.
ACCESSION   DASUEV010000053 DASUEV010000000
VERSION     DASUEV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130888"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1193)
                     /locus_tag="VFV55_01735"
     CDS             complement(21..1193)
                     /locus_tag="VFV55_01735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4943040.1"
                     /translation="MSATIPGQFGRFRVLEELGRGAMGVVYRAQDPALGRTVAIKTIT
                     LTGTDEERAQFEARFMQEARAAGGIGHPSIITIYDVGREGDVAFMAMELLDGQELRDL
                     IRGGSLAPSEAVEIAAQVAEALSAAHERGVIHRDVKPGNIMVLSDGRVKIMDFGIARL
                     REPAVKTATGMMLGSPRYMAPEQVSGQAFDHRADIFSLGVVLYEMLTGITPFAGGDLT
                     QLMFQVVNAAVVPPSHVNRTLPPVLDYILARALKKNPDERYGSAAELATDLRDSIADV
                     LEAEAAVRARTDASGTGTVPDLPGWGTAQRGAPSVDRGDEVVELRPSPRFDCVEGLAR
                     LAVLPVDSDATRSRAGYTLPAGTKPQRAPWRSDLPFVLVVAGYLAATVVAVIIVLR"
     gene            complement(1196..2086)
                     /locus_tag="VFV55_01740"
     CDS             complement(1196..2086)
                     /locus_tag="VFV55_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943041.1"
                     /translation="MRTAAPAFANIAFLKIARFGERPIVEQARLKDELEARVRTAIAG
                     IPVADRIVLDAADGIALVLFGEPARALEMAQSLRSRPGNEPLKVGLNHGPIAVTARDA
                     GAMVFGDGISAAAAAARFATGERLLVTAPFAKMLEATRPDRAAELAPAGEFTDARVRL
                     HSFFTPEPRRLVARRNRFTAYALAGMLGILLFGVATRMVSRTFFLPRPAVIEFEVKPR
                     GEVVIDGHSRGRTPPLREVELASGKHHVQVRSGNLPPVDLKVDLEPGQRMTITHNFAG
                     ERAAAKPGGFWRDLRRNLGF"
     gene            complement(2148..>2363)
                     /locus_tag="VFV55_01745"
     CDS             complement(2148..>2363)
                     /locus_tag="VFV55_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943042.1"
                     /translation="QSVMLAAQARGLATCAQGALASWAGPPKRAFDVPGDFMLVCGLS
                     IGYASQDPVNAFDPGRAPIGELLIAAR"
BASE COUNT          373 a          827 c          821 g          342 t
ORIGIN      
        1 gtaacctgac cccttttcct tcagcgaagc acgatgatca ccgcgacgac ggtcgcggcg
       61 aggtagccgg ccaccaccag gacgaacggc aggtccgatc gccacggcgc gcgctgcggc
      121 ttcgtgcccg cgggcagcgt gtatccggca cgcgaacgcg tcgcatccga gtcgaccggc
      181 agcaccgcga gccgcgccag gccctcgacg caatcgaagc gcggtgacgg acgcaactcg
      241 actacctcgt caccacgatc cacggagggc gcgccgcgtt gcgcggtgcc ccatccgggg
      301 aggtcgggca cggtgcccgt gcccgaggca tcggtgcgcg cccgcaccgc cgcttccgcc
      361 tcgagcacgt cggcgatcga atcgcgcagg tcggtggcga gttccgcggc gctgccatag
      421 cgctcgtcgg ggttcttctt gagcgcgcgc gcgagaatgt agtcgagcac cggcggaagc
      481 gtccggttga cgtggctggg cgggaccacc gccgcgttca ccacctggaa catgagctgc
      541 gtgaggtcgc cgcccgcgaa gggcgtgatt cccgtgagca tttcgtacag gacgacgccc
      601 agcgaaaaga tgtcggcgcg atggtcgaac gcctgtcccg agacctgctc gggcgccatg
      661 tagcgcggcg accccagcat catcccggta gcggtcttca ccgccggctc gcgcagcctc
      721 gcgatgccga agtccatgat cttcacgcgc ccgtccgaga gcaccatgat gttgcccggc
      781 ttcacgtcgc gatggatcac gccacgctcg tgcgcggcgg agagtgcctc ggccacctgg
      841 gcggcgatct ccaccgcctc cgacggcgcg agcgagccgc cgcgaatcag atcgcgcagc
      901 tcctgcccgt cgagcagttc catcgccatg aaggccacgt cgccctcgcg gcccacgtcg
      961 tagatcgtga tgatcgacgg atggccgatt ccgccggccg cccgcgcttc ctgcatgaag
     1021 cgcgcctcga attgcgcccg ttcctcgtcg gtgccggtga gcgtgatcgt cttgatcgcc
     1081 acggtgcggc ccagggcagg gtcctgcgcg cggtagacca cgcccatcgc gccgcgaccc
     1141 agttcctcga ggacacggaa gcggccgaat tgcccgggga tcgtcgcgga catcgtcaga
     1201 acccgagatt gcggcgcagg tcacgccaga agccgcccgg cttcgcggcc gcccgctcgc
     1261 cggcaaagtt gtgggtgatc gtcatgcgct gtcccggctc gaggtccacc ttcaggtcga
     1321 caggcgggag attgccgctt cgcacctgca cgtggtgctt gcccgaggca agctccacct
     1381 cgcgcaaggg cggcgtgcgc ccgcgcgaat gaccgtcgat caccacctcg ccgcgtggct
     1441 tcacctcgaa ctcgatcact gcggggcgcg gcaggaagaa cgtccggctc accatccggg
     1501 tggcgacgcc gaacagcaaa atgcccagca tgccggccag cgcgtacgcg gtgaagcggt
     1561 tgcggcgagc aacgaggcgc cggggctccg gcgtgaaaaa ggaatgcagg cgcacgcggg
     1621 cgtcggtgaa ttctcccgcc ggggcaagtt cggcggcgcg atcgggccgc gtggcctcga
     1681 gcatcttcgc gaagggagcg gtgacaagca atcgctcccc ggttgcgaag cgggcggccg
     1741 cggccgcggc ggagatgccg tcgccgaaca ccatcgcccc cgcgtcgcgc gcggtgacgg
     1801 cgatggggcc gtggttgagg ccgaccttga gcggctcgtt gcccggtctc gagcgaaggg
     1861 attgcgccat ctcgagggcg cgggcgggct ccccgaaaag aaccagtgcg attccgtcgg
     1921 cggcgtccag gacgatgcgg tcggcaacgg gaattcccgc gatggcggtg cgaacgcgag
     1981 cttccagttc gtccttgagg cgcgcctgct ccacgatggg tcgctcgccg aagcgggcga
     2041 tcttcaggaa cgcgatgttg gcgaaggcag gggcagcggt gcgcatgcgg cacaagcagt
     2101 gggtttgcgc ccattgtaga cgccggcgac cgccccccgt ggcgcgttca tcgcgccgcg
     2161 atcagcaatt cgccgatcgg cgcccgtccc ggatcgaacg cgttcaccgg gtcctgcgat
     2221 gcatagccga tcgaaagccc gcagacgagc atgaagtcgc cgggcacgtc gaacgcgcgt
     2281 ttcggcggcc ccgcccatga ggcgagcgcc ccctgcgcgc aagtggcgag tccccgcgcc
     2341 tgcgccgcca gcatgaccga ctg
//
LOCUS       DASUEV010000054         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1047815, whole genome shotgun sequence.
ACCESSION   DASUEV010000054 DASUEV010000000
VERSION     DASUEV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047815"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFV55_01750"
     CDS             <1..220
                     /locus_tag="VFV55_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4943043.1"
                     /translation="TGEHGVGIEKLKQMCLQFRGAEIAAFHDVKRAFDPAGILNPGKA
                     VPTLKRCVEWRGTHVSDGRIPYPDIPRF"
     gene            247..1305
                     /gene="glcE"
                     /locus_tag="VFV55_01755"
     CDS             247..1305
                     /gene="glcE"
                     /locus_tag="VFV55_01755"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX4943044.1"
                     /translation="MADDFIQTLADRVKDHAARKAPLRIRGGGTKDFYGARTDGEPLD
                     IAPYAGIVAYEPRELVLTVKAGTRLVDIEAALAAEGQMLPFEPPHFGDAATIGGTVAG
                     NFSGPRRPYAGAARDFVLGARIVNGKGEDLSFGGRVIKNVAGYDVSRLMAGALGTLGV
                     LTELSFKVLPKPPAEATLALEMDEARMIETVNKWAGQPLPLSATAWEAGVLRVRLSGA
                     SSAVAAARARLGGSDVADGAAYWDALREHRLPFFAMDKPLWRLFVAQTAAPVRFAHPQ
                     LVEWGGGQRWVSGALEPGNARKAAAGAKGHATLFRGGDRSAGVFHPLAPAIAKIHGRL
                     KAAFDPAGILNPGRMDNF"
     gene            1315..2559
                     /gene="glcF"
                     /locus_tag="VFV55_01760"
     CDS             1315..2559
                     /gene="glcF"
                     /locus_tag="VFV55_01760"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX4943045.1"
                     /translation="MQTNLADWIKDTPQGREADAILRKCVHCGFCLATCPTYNLLGDE
                     LDSPRGRIYLMKQVLEGSPVTAKTQVHLDRCLTCRACETTCPSGVQYGRLVDIGREVV
                     DRKVPRGFADRALRRMLRFAFLSGPLFAFGVGVGRVLRPLLPASLARQVPPSRKAGAW
                     PPARHARKMIVLDGCVQPTLAPDINAATARVLDRLGISLVSVPQSGCCGAIEYHLNAQ
                     DAALERIRRQVDAWWPHVEAGAEAIVVTASGCGTMVQEYAHLLAGDTAYAKKAARITE
                     LAKDVGQVVAAERETLAKLLAGMAPVATPVAYHAPCSLQHGLKQKGIVEAVLREAGFS
                     LTFVPDSHVCCGSAGTYSILQPELSGQLLANKVKALESEGATAICTSNIGCLLHIETG
                     ASKPVSHWIELLDGRLAEARTP"
     gene            2599..>4232
                     /locus_tag="VFV55_01765"
     CDS             2599..>4232
                     /locus_tag="VFV55_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4943046.1"
                     /translation="MPAQDEKRHGAASGIADREAPSSRDGDRSDVERALFDVVRETCR
                     DLHRQPKRRVDVGLDSSFDRDLGLDSLARVELLLRVERAFKVRLAEDTLALAETPRDL
                     LAALRLATDTAGVRPSATAVARPGESVHGEPVDATTLPEVLAWHVRRHPGRTQIIVSS
                     AEGEEEISYGKLMLEATDVAARLQALGLEAKQSVALMLPTGPAYFYAYFGVLLAGGVP
                     VPIYPPARPSQIEDHVRRHARILSNAQAVILVTVPEAMPVARLLKTVVHGLRDVIAMP
                     GRPGESGKSSEVAVRSEEVAFIQYTSGSTGNPKGVVLTHANLLANIRAIGEAVAINGD
                     DVFVSWLPLYHDMGLIAAWLASLYYGNPLVALSPLAFLARPESWLWAIHRHRGTLTAA
                     PNFAYELCLKRIDDAQLEGLDLGSLRLAANGAEPVSPETVERFIGRFAKFGFRREAMT
                     PVYGLAEATVALLAPPLGRGPLVDRVRREPFAGERKAVPAAPDDTNPLRFVACGRPLP
                     GHRVRIVDEAGAEVGERVEGRLEFHGPSATAGYHRNP"
BASE COUNT          668 a         1471 c         1436 g          657 t
ORIGIN      
        1 caccggcgag cacggcgtcg gcatcgagaa gctgaagcag atgtgccttc agttccgcgg
       61 tgccgagata gccgccttcc acgacgtcaa gcgcgccttc gatcctgccg gcatcctcaa
      121 tccgggcaag gccgttccca cgctcaagcg ctgcgtggag tggcgcggca cgcatgtgag
      181 cgacggccgc atcccgtatc ccgacatccc ccgcttctga aaaagggaca gtccctaatc
      241 ttgtccatgg cagacgattt cattcaaacg cttgcagacc gggtgaagga tcacgcggcg
      301 cgcaaggccc cgcttcgcat ccgcggcggc ggcacgaagg acttctacgg cgcgcgcacg
      361 gacggcgagc cgctggacat cgcgccttac gccggcatcg tggcctacga accgcgcgaa
      421 ctcgtgctca ccgtgaaggc gggcactcgc ctggtggaca tcgaggccgc gctcgctgcc
      481 gaaggccaga tgctcccctt cgagcctcct cacttcggcg acgcggcgac catcggcggc
      541 acggtcgccg gcaatttctc cgggccgcgc cgtccctacg cgggtgccgc gcgcgacttc
      601 gtgctcggtg cgcgcatcgt gaacggcaag ggcgaggacc tctccttcgg cgggcgtgtc
      661 atcaagaacg tggccggcta tgacgtctcg cggctcatgg cgggcgcgct cggcacgctg
      721 ggcgtgctca cggagctctc cttcaaggtg ctgccgaaac cgcccgcgga agcgacgctc
      781 gccctggaga tggacgaggc gcgcatgatc gagacggtca acaagtgggc cggccagccg
      841 cttccgctct ccgccacggc atgggaggcg ggcgtgctgc gtgtccggct ctcgggcgcg
      901 tcgagcgcgg tggccgcggc acgcgcaaga ctgggcggga gcgatgtcgc cgacggcgcc
      961 gcgtactggg acgcgcttcg cgagcatcgc ctgcccttct tcgcgatgga caagcccctc
     1021 tggcgccttt tcgtcgcgca gacggcggca cccgtcaggt tcgcgcatcc ccagctcgtc
     1081 gagtggggcg gcggccagcg ctgggtcagc ggtgcgctcg agccgggcaa cgcgcgcaag
     1141 gcggcggccg gcgcgaaggg ccacgcgacg ctcttccgcg gtggcgaccg gtcggccggc
     1201 gtgttccacc ccctggcgcc cgccatcgcc aagatccacg gacgcctcaa ggcggcgttc
     1261 gaccccgccg gcattctcaa ccccggccgc atggacaact tctgaccgcc cgcgatgcaa
     1321 accaacctcg ccgactggat caaggacacg ccgcaaggcc gcgaagccga cgcaatcctg
     1381 cgcaagtgcg tgcactgcgg gttctgcctg gccacctgcc ccacctacaa cctgctgggc
     1441 gacgaactgg acagcccgcg cgggcgcatc tatctcatga aacaggtgct cgagggttcg
     1501 ccggtgacgg cgaagaccca ggtgcacctc gaccgctgcc tcacctgccg cgcctgcgag
     1561 acgacgtgcc cctcgggcgt gcaatacggc cggctcgtcg atatcgggcg ggaggtcgtc
     1621 gaccgtaaag tgccgcgcgg tttcgccgac cgcgcattgc gccggatgct gcgcttcgcg
     1681 ttcctgtccg ggccgctctt cgcgttcggc gtgggcgtgg gccgcgtctt gcgccccttg
     1741 cttcccgcat cgctcgcgag gcaagtgccg cccagccgga aggcgggagc gtggccgccg
     1801 gcgcggcatg cgcggaagat gatcgtcctc gatggctgcg tgcagcccac gctcgcaccg
     1861 gacatcaatg ccgccacggc gcgcgtgctc gaccggctgg gcatctcgct ggtatccgtg
     1921 ccgcaatcgg gatgctgcgg cgccatcgaa taccacctca acgcgcagga tgccgcgctc
     1981 gaacgcatcc gccgccaggt cgacgcctgg tggccgcacg tcgaggccgg tgcggaagcg
     2041 atcgtcgtca ccgcgagcgg ttgcggcacc atggtgcagg aatatgcgca tctgctcgcg
     2101 ggcgacaccg catacgcgaa gaaggcggcg cgcatcaccg aactcgcgaa ggacgtggga
     2161 caggttgtcg ccgccgaacg cgagaccctg gcgaagctgc tggcgggaat ggcaccggtt
     2221 gcgactcccg tggcctacca cgctccctgc agcctgcaac acggcctcaa gcagaaggga
     2281 atcgtggagg cggtcctgcg cgaggccggt ttctctctca ccttcgtgcc ggattcgcac
     2341 gtgtgctgcg gctcggcggg aacctactcg atcctccagc cggaactgtc gggccagctc
     2401 cttgccaaca aggtgaaggc gctcgaatcg gaaggggcca ctgccatctg cacctcgaac
     2461 atcggttgcc tgctgcatat cgagacgggc gcctcgaagc cggtgtcgca ctggatcgaa
     2521 cttctggacg ggcgcctggc ggaggcgcgc acgccgtagc cgccgcagcg cgggttccgt
     2581 cggcacatcg gaggccgaat gccggcccag gacgagaaaa ggcacggtgc cgcaagcggg
     2641 atcgccgacc gcgaagcgcc ctcgtcccgg gacggcgatc gaagcgacgt cgagcgcgcc
     2701 ctgttcgacg tagtgcgcga gacgtgccgg gatctgcaca ggcaaccgaa gcgccgtgtc
     2761 gatgtcgggc tggacagttc gttcgaccgc gacctcggcc tagacagcct ggcgcgggtc
     2821 gagctcctgc tccgtgtcga gcgcgccttc aaggtccgcc tcgccgaaga cacccttgcc
     2881 cttgccgaaa cgccgcgcga cctgctggcg gcgttgcgcc ttgcgacgga caccgcgggc
     2941 gtccgacctt cggcaacagc cgtcgcgagg ccgggcgaat cggtccacgg cgaaccggtc
     3001 gatgcgacga cgttgccgga ggtccttgcc tggcacgtgc gccgccatcc cggccgtacc
     3061 cagatcatcg tctcctccgc ggagggcgag gaggagatct cgtatggcaa gctgatgctg
     3121 gaggcaacgg acgttgcggc gcggcttcag gcgctggggc tggaggcgaa gcaatcggtg
     3181 gccctcatgc tgcccacggg ccccgcgtat ttctacgcct atttcggcgt tctcctggcg
     3241 gggggcgtac ccgtcccgat ctatccgccg gcgcgacctt cccagatcga ggaccatgtg
     3301 cgccgccatg cgcgcatcct ctccaatgcc caggcggtga tcctggtcac cgtgcccgag
     3361 gcgatgccgg tggcccgtct tctcaagact gtggtgcacg gattgcggga cgtgatcgca
     3421 atgcccggcc gaccggggga aagcggcaag tccagcgagg tggcggtcag gtccgaagaa
     3481 gtcgccttca tccagtacac gtccggaagt accggaaacc cgaagggtgt cgtactcacc
     3541 cacgccaacc tgctggccaa catcagggcc atcggggagg cggtagccat caacggggac
     3601 gacgtgttcg tcagctggct gcccctttac cacgacatgg gactcatcgc cgcgtggctc
     3661 gccagcctgt attacggaaa tccgctcgtc gccctgtcac cgctggcgtt cctggcgcga
     3721 ccggagagct ggctgtgggc catacaccgc catcgcggga ccctcacggc cgcgcccaac
     3781 ttcgcctacg agctttgcct gaagcgaatc gacgacgcgc aactcgaggg cctggacctg
     3841 gggtcgcttc gactcgcggc caacggggcg gagcccgtca gtcccgaaac ggtggaacga
     3901 ttcatcgggc gattcgcgaa gttcggattt cggcgcgaag cgatgacgcc ggtctacggc
     3961 cttgccgaag cgacggttgc actgctggcg cccccattgg gccgtggtcc cctggtggat
     4021 cgcgtgcgcc gcgaaccgtt cgcgggtgaa cggaaggcgg ttccagctgc gccggatgac
     4081 accaacccgt tgcgcttcgt cgcttgcggc aggcccttgc cggggcaccg cgtacgcatc
     4141 gtcgacgagg ccggcgcgga agtcggtgaa cgcgtggaag ggcggctgga attccacggg
     4201 ccgtccgcga ccgccggcta ccatcgcaat cc
//
LOCUS       DASUEV010000055         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_818623, whole genome shotgun sequence.
ACCESSION   DASUEV010000055 DASUEV010000000
VERSION     DASUEV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818623"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VFV55_01770"
     CDS             <1..137
                     /locus_tag="VFV55_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX4943047.1"
                     /translation="KTSRDQFEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEIKL
                     "
     gene            248..907
                     /gene="rplC"
                     /locus_tag="VFV55_01775"
     CDS             248..907
                     /gene="rplC"
                     /locus_tag="VFV55_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX4943048.1"
                     /translation="MSLGLVGRKVGMMRIFDEDGTSVPVTVLDCAGNRVTQIKTADGD
                     GYCAIQVAYGKRRATRVTKAMAGHFAKAGAEAGSVLKEFRVAPEALAELAVGAELKVD
                     MFQVGQKVDITGTSIGKGYAGVIKRHHFSSNRASHGNSRSHNSPGSIGMAQDPGRVFP
                     GKRMAGHLGNVQRTVQLLEVARVDAERGLVMVKGSVPGAKNGSVVLRPSVKAKTPKGG
                     K"
     gene            907..1527
                     /gene="rplD"
                     /locus_tag="VFV55_01780"
     CDS             907..1527
                     /gene="rplD"
                     /locus_tag="VFV55_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX4943049.1"
                     /translation="MELKVINDKGEALAPVAASEALFGRSFNEALVHQVVVAYHANGR
                     AGTRAQKDRGNVKHSTKKPWRQKGTGRARSGMTSSPLWRGGGKIFPNSPDENFTHKLN
                     RKMYRAGMASILSQLVREERLSVVDALTVDQPKTKLLSQKLKAMGLTEVLIITDQPDE
                     NLYLSSRNLPNVLVLEARDADPVSLVRFPRVLLTRGAVKHFEEALA"
     gene            1524..1832
                     /gene="rplW"
                     /locus_tag="VFV55_01785"
     CDS             1524..1832
                     /gene="rplW"
                     /locus_tag="VFV55_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX4943050.1"
                     /translation="MNTERLMTVIRAPIISEKATLVGEKHRQIVFEVLADATKAEIKA
                     AVELLFKEQKVEVAAVNVVNLKGKQKRHGRFEGRRRDVKKAYVSLKGEGDINFVEGVA
                     "
     gene            1834..2655
                     /gene="rplB"
                     /locus_tag="VFV55_01790"
     CDS             1834..2655
                     /gene="rplB"
                     /locus_tag="VFV55_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX4943051.1"
                     /translation="MALVKTKPTSAGRRSMVKVVNPDLHKGKPHAPLVEKQSRRAGRN
                     SFGNITTRHQGGGHKQHYRLIDFRRNKDGIPATVERLEYDPNRSANIVLLCYADGERR
                     YMIAPKGITVGQKIESGAEAPIKTGNALPLRNIPVGTTVHCVEMMPGKGAQIARAAGA
                     GVQLLAREGEYAQLRMRSGEIRRVHVDCRATIGEVGNEEHNLRQIGKAGANRWRGIRP
                     TVRGETMNPVDHPLGGRTRGNRHPVSPWGQPAKGKKTRNNKRTQTMIVRSRHKKA"
     gene            2668..2964
                     /gene="rpsS"
                     /locus_tag="VFV55_01795"
     CDS             2668..2964
                     /gene="rpsS"
                     /locus_tag="VFV55_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX4943052.1"
                     /translation="MSRSIKKGPFVDGHLMKKVGAAVAAKDKRPIKTWSRRSTVLPDF
                     VGLTIAVHNGRQHIPVYVTENMVGHKLGEFALTRTFKGHSADKKVAAPSAPPKK"
     gene            2976..3314
                     /gene="rplV"
                     /locus_tag="VFV55_01800"
     CDS             2976..3314
                     /gene="rplV"
                     /locus_tag="VFV55_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX4943053.1"
                     /translation="MQVDAKLYGAKLSAQKGRLVADMVRGQKVDRALNILAFTPKKGA
                     VIIKKVLESAIANAEHNNGADIDELKITRIMVEKGPVLKRFHARAKGRGNRVLKHTCH
                     VFLTVGDGRN"
     gene            3326..>3682
                     /gene="rpsC"
                     /locus_tag="VFV55_01805"
     CDS             3326..>3682
                     /gene="rpsC"
                     /locus_tag="VFV55_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX4943054.1"
                     /translation="MGQKINPTGFRLSVNRNWSSKWYANSKNFPGMLAEDIRVREYLK
                     KKLSHAAVGKVVIERPAKNARITIYSARPGVVIGKKGEDIEGLRHVLQKLMGVPVHVN
                     IEEIRKPELDAQLIADS"
BASE COUNT          751 a         1234 c         1231 g          466 t
ORIGIN      
        1 acaagacctc gcgcgaccag ttcgagatcc gcacgcacct gcgcctcatg gacatcatcg
       61 acccgacgga caagacggtc gacgcgctga tgaagctcga tctccccgct ggcgtcgacg
      121 tcgagatcaa gctgtaggaa gttgcagggc cgcccgggca gccgcccgga cggaaggtga
      181 agggccggcc aattgcagcc ggcacccgcg ggcaagccgg cgggcgaaca agggaaagga
      241 tttgaagatg agtctcggac tcgtcggccg caaggtcggc atgatgcgca tcttcgacga
      301 ggacggcacc agcgtccccg ttacggtgct cgactgcgcg ggaaaccgcg tcacccagat
      361 caagaccgcc gatggcgacg gctactgcgc catccaggtc gcctatggca agcgccgcgc
      421 cacgcgcgtg accaaggcca tggccggaca cttcgccaag gccggcgccg aggcgggcag
      481 cgtcctcaag gagtttcgcg tcgcccccga ggcgctcgcc gagctcgccg tgggcgcgga
      541 actcaaggtg gacatgttcc aggtcggcca gaaggtcgac atcaccggga cctccatcgg
      601 caagggctac gccggcgtca tcaagcgcca ccacttctcc tcgaaccgcg cctcgcacgg
      661 caactcgcgc tcgcacaaca gcccaggttc gatcggcatg gcgcaggacc cgggccgcgt
      721 gttccccggc aagcgcatgg ccggccacct cggcaacgtc cagcgcaccg tccagctcct
      781 ggaagtggcg cgcgtcgacg ccgagcgcgg cctcgtcatg gtcaagggct cggttccggg
      841 cgccaagaac ggcagcgtgg tgctccgccc gagcgtgaag gccaagacgc cgaagggagg
      901 caagtgatgg aactcaaggt catcaacgac aagggcgagg cactcgcccc ggtggccgcc
      961 tccgaggccc tgttcgggcg ctcgttcaac gaggcgctcg tccaccaggt cgtggtcgcg
     1021 taccacgcca acggccgcgc gggaacgcgc gcgcagaagg accgcggcaa cgtcaagcac
     1081 tcgacgaaga agccgtggcg ccagaagggc accggccgcg cgcgctcggg catgacgtcg
     1141 agcccgctct ggcgcggcgg cgggaagatc ttccccaact cgccggacga gaacttcacg
     1201 cacaagctca accgcaagat gtaccgcgcg ggcatggcct cgatcctgtc gcagctcgtt
     1261 cgcgaggagc ggctgtcggt ggtcgacgcc ctcacggtgg accagcccaa gacgaagctc
     1321 ctgtcgcaga agctcaaggc gatggggctc accgaggtgc tgatcatcac cgaccagccc
     1381 gacgagaacc tctatctttc gtcgcgcaac ctgcccaacg tgctggtgct cgaggcgcgc
     1441 gacgcggacc cggtgagcct ggtccggttc cccagggtcc tgctgacccg cggcgcggtg
     1501 aagcacttcg aggaggcgct cgcatgaata ccgaacgact gatgaccgtc atccgcgccc
     1561 cgatcatctc ggagaaggcg acgctggtgg gcgagaagca ccgccagatc gtcttcgagg
     1621 tcctcgcgga cgcgaccaag gccgagatca aggccgccgt ggagctgctc ttcaaggagc
     1681 agaaggtgga ggtcgccgcg gtgaacgtgg tgaacctcaa gggcaagcag aagcgccacg
     1741 ggcgcttcga gggccggcgc cgcgacgtga agaaggccta cgtgagcctc aagggtgaag
     1801 gcgacatcaa cttcgtggaa ggagtggcat aacatggcac tcgtcaagac caagcccacc
     1861 tcggccggcc gccggtcgat ggtgaaggtc gtcaatcccg acctgcacaa gggcaagccg
     1921 cacgccccgc tggtggaaaa gcagtcgcgg cgggccggcc ggaactcctt cggcaacatc
     1981 accacgcgcc accagggcgg cgggcacaag cagcactatc gcctgatcga cttccgccgc
     2041 aacaaggacg gcatcccggc cacggtcgag cggctcgagt acgacccgaa ccgcagcgcc
     2101 aacatcgtgc tgctctgcta cgcggacggc gagcgccgtt acatgatcgc gcccaagggg
     2161 atcaccgtcg ggcagaagat cgagagcggc gccgaggcgc cgatcaagac cggaaacgcg
     2221 ctgccgctgc gcaacatccc ggtgggcacg acggttcact gcgtcgagat gatgccgggc
     2281 aagggcgcgc agatcgcgcg tgccgccggc gccggcgtgc agctgctggc ccgcgagggc
     2341 gagtacgcgc agctgcgcat gcgctccggc gagatccgcc gcgtccacgt cgactgccgc
     2401 gccacgatcg gcgaggtggg caacgaggag cacaacctgc gccagatcgg caaggccggc
     2461 gccaaccgct ggcgcggcat ccgcccgact gtccgcggcg agacgatgaa cccggtcgac
     2521 cacccgctgg gcggccggac gcgcggcaac cggcacccgg tctccccgtg gggacagccg
     2581 gccaagggca agaagacgcg aaacaacaag cgcacgcaga cgatgatcgt gcgcagccgg
     2641 cacaagaagg cgtaaggggc caaccacatg tcacgttcaa tcaagaaggg cccgtttgtc
     2701 gacgggcatc tcatgaagaa ggtgggggcc gccgtcgcgg ccaaggacaa gcgtccgatc
     2761 aagacctggt cgcgccgttc caccgtcctg ccggatttcg tcggcctcac gatcgcggtc
     2821 cacaacggca ggcagcacat tcccgtctac gtgacggaaa acatggtcgg ccacaagctg
     2881 ggcgagttcg cgctgacgcg caccttcaag ggccactcgg ccgacaagaa ggtcgcggcg
     2941 ccgtcggccc cgccgaagaa gtgaggagaa acgcgatgca agtagacgca aagctttacg
     3001 gcgccaagct ctctgcccag aagggccggc tggtcgccga catggtgcgc ggccagaagg
     3061 tcgaccgcgc gctcaacatc ctcgccttca ccccgaagaa gggcgccgtg atcatcaaga
     3121 aggtgctcga gagcgcgatc gcgaacgccg agcacaacaa cggcgccgac atcgacgagc
     3181 tgaagatcac ccgaatcatg gtcgagaagg gaccggtgct caagcgcttc catgcgcgcg
     3241 ccaaggggcg cggcaaccgc gtgctgaagc atacctgcca cgtgttcctc accgtcggcg
     3301 acggcaggaa ctaaagggga atacgatggg acagaagatc aatccgaccg gattccgcct
     3361 ctcggtcaac cggaactggt cgtcgaagtg gtacgcgaac tcgaagaact tccccgggat
     3421 gctcgccgag gacatccggg tgcgcgagta cctgaagaag aagctctccc acgccgcggt
     3481 cggcaaggtc gtcatcgagc gccccgccaa gaacgcgcgc atcacgatct actcggccag
     3541 gccgggcgtg gtgatcggca agaagggcga ggacatcgag ggcctgcgcc acgtcctgca
     3601 gaagctcatg ggcgtgccgg tgcacgtgaa catcgaggag atcaggaagc ccgagctgga
     3661 cgcccagctc atcgccgact cg
//
LOCUS       DASUEV010000056         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_131625, whole genome shotgun sequence.
ACCESSION   DASUEV010000056 DASUEV010000000
VERSION     DASUEV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131625"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..801)
                     /locus_tag="VFV55_01810"
     CDS             complement(157..801)
                     /locus_tag="VFV55_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX4943055.1"
                     /translation="MELIAGLLDIVLRLDVHLGALVSDYGAWVYAILFAVIFAETGLV
                     VTPFLPGDSLLFVAGALAALGGLEVHLLVAALTAAAVLGNTTNYAIGRWLGRRYFDGA
                     GSRWIRREHLERTHAFYERYGGAAVVISRFLPIIRTYVPFVAGLAQMGSARFTAYNVG
                     GALLWVGSLSYAGYFFGNLPWVRANLTLIIVGIIAASLVPIAVAAWKARGEAQP"
     gene            complement(806..1759)
                     /gene="secF"
                     /locus_tag="VFV55_01815"
     CDS             complement(806..1759)
                     /gene="secF"
                     /locus_tag="VFV55_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX4943056.1"
                     /translation="MEFFKIRRTIPFMRHALVFNVISLVTFLLAVGFLATRGLHLSIE
                     FTGGTVMEVTHDKPAQPDHIRDSLDAAGFKDINVIKFGSSQDVLIRLPLKANPDPKAQ
                     NEEMQKQSAAAMQVLKGVDPSARLVRTEFVGGQVGKELATDGALALLVVCVGIMIYLA
                     FRFEWRFAVATIIANLHDVVIILGVFAAFQWEFSLPVLAAVLAILGYSVNESVVIFDR
                     VRENFRKMRRASVDEIMDASITGTISRTIITHGSTQMMVLSILFFGGASLYYFALALT
                     IGICFGIYSSVLVAAPLAKYLGASREDFVKPEKKTVEPVVP"
     gene            complement(1798..>3529)
                     /gene="secD"
                     /locus_tag="VFV55_01820"
     CDS             complement(1798..>3529)
                     /gene="secD"
                     /locus_tag="VFV55_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX4943057.1"
                     /translation="EPMRTGIEAAIKGAGIALAGVDDSEGQATFRFADTDTQIKARDI
                     IQSKVPPGYVVALNLQSSTPGWLQALGAKPMYLGLDLRGGVHFLLQVDMKAAASKSVE
                     RYVGDVRSLLRDKKIYYSGLAREGDRVVVRFRERDQRLEAQKLIEKEMPDLAIREQDA
                     GEEAAIVATIKPEVVKKEQELALQQNIQTLRNRVNELGVAEPIIQQQGADRIVVQLAG
                     VQDPGRAKEILGRTATLEVRLVSEEHAAGPGQNAFIAYKDQPAPFGTDKFFDTEGNAV
                     LVKKMLVITGDRIKDAQPGFDQRDGRPVVSMSLDDTGGRIMRQTTRENLKKRMGMMLV
                     ERNSTTVLTWPVIQEEFGNRFQISGIGDVQEARNLALLLRAGALAAPMDIIEERTVGP
                     SLGAENIAKGFRSVLYGFAAIAAFMIVYYVLFGVISVAALASNLLFLVALLSMLQATL
                     TLPGIAAIALALGMAIDANVLINERIREELRSGMSPQMAIQVGYERAFDTIFDSNITT
                     LIAGLSLLAFGSGPVRGFAVVHCLGILTSIFSAVMVSRGIVNLIYGGRKKVVAVSIGN
                     TQWHKKDATA"
BASE COUNT          580 a         1184 c         1191 g          574 t
ORIGIN      
        1 agacgtgttt ggctggcccc gactttttgg gatccattcg acggtcacgt ttgcgtgccc
       61 aggacgccga tagacttgcc ccagccccgg ccctcctccc attcgccgca tgcatcaggc
      121 gccgccggga tcgtgtcgcg cgactctgga ctcgcgtcag ggctgcgctt cgccccgcgc
      181 cttccacgcc gccactgcga tcggcacgag gctcgccgcg atgatcccca cgatgatgag
      241 ggtgaggttg gcccgcaccc aggggaggtt gccgaagaag tagcccgcgt aggagagcga
      301 gccgacccac aggagcgcac ctccgacgtt gtacgcggtg aaccgggccg aacccatttg
      361 cgcgagcccg gccacgaagg ggacgtaggt cctgatgatc ggcaggaagc gcgagatcac
      421 caccgcggcc ccgccgtagc gttcgtagaa tgcgtgcgtg cgctcgaggt gctcgcgcct
      481 gatccagcgc gagcccgcgc cgtcgaagta ccgcctgccg agccatcggc cgatcgcgta
      541 attggtggtg ttgccgagaa cggccgcggc cgtgagcgcc gcgacgagga ggtgcacctc
      601 gagccccccg agggcggcca gcgccccggc cacgaagagg agcgagtcgc cgggcaggaa
      661 cggggtgacg acgagccccg tttccgcgaa gatcaccgcg aagaggatcg cgtagaccca
      721 cgcgccgtag tcgctgacga gggcgcccag gtggacatcg agccgcagga cgatgtccag
      781 gagccccgcg atcagctcca cgtcctcagg gcaccacggg ctcgacggtc ttcttctccg
      841 gcttcacgaa gtcctcgcgc gatgcgccga ggtacttggc gagcggcgcg gccaccagca
      901 ccgaggagta gatgccgaag cagatgccga tggtgagcgc gagcgcgaag tagtacaggc
      961 tcgccccccc gaagaagagg atcgagagca ccatcatctg cgtcgagccg tgggtgatga
     1021 tcgtgcgcga gatcgtgccg gtgatggagg cgtccatgat ctcgtccacc gaggcgcggc
     1081 gcatcttgcg gaagttctcg cgcacccggt cgaagatcac cacggattcg ttcaccgagt
     1141 agcccaggat cgcgagcacc gcagccagca ccggcagcga gaattcccac tggaacgcgg
     1201 cgaacacgcc gaggatgatg accacgtcgt gcaggttcgc gatgatggtg gccaccgcga
     1261 agcgccactc gaagcggaag gcaaggtaga tcatgatgcc cacgcacacc accagcagcg
     1321 ccagagcccc gtcggtcgcg agctccttgc ccacctggcc gcccacgaac tcggtgcgca
     1381 ccagccgggc ggaggggtcc acgcccttga gcacctgcat cgcggcggca ctctgcttct
     1441 gcatctcctc gttctgcgcc ttggggtccg ggttcgcctt gagcggcagc cggatcagca
     1501 cgtcctgcga cgagccgaac ttgatcacgt tgatgtcctt gaatcccgcc gcatcgagcg
     1561 aatcgcggat gtggtcgggc tgcgcgggct tgtcgtgcgt cacctccatc accgtgccgc
     1621 cggtgaactc gatggagagg tgcaggcccc tcgtggcgag gaagcccacg gccagcagga
     1681 acgtgacgag cgagatcacg ttgaacacca gcgcgtggcg catgaagggg atcgtgcgcc
     1741 ggatcttgaa gaattccatg gtgtggttcc cgggtcttta gcgcttcagg cggccgttca
     1801 ggccgtcgcg tccttcttgt gccactgcgt gtttccgatg gacaccgcga cgaccttctt
     1861 ccggccgccg tagatcaggt tgacgatgcc gcgcgagacc atcaccgcgc tgaagatgga
     1921 ggtcaggatg cccaggcagt gcaccaccgc gaagccgcgc accgggccgg agccgaacgc
     1981 cagtagcgac aggcccgcga tgagggtggt gatgttggag tcgaagatgg tgtcgaaggc
     2041 gcgctcgtag cccacctgga tggccatctg cgggctcatg cccgagcgca actcctcgcg
     2101 gatcctctcg ttgatgagca cgttggcgtc gatcgccatg ccgagcgcca gcgcgatggc
     2161 cgcgatgccc gggagcgtga gggtggcctg caacatcgac agcagcgcca cgaggaacag
     2221 caggttggac gccagcgccg ccaccgagat gacgccgaag aggacgtaat acacgatcat
     2281 gaacgcggcg atggccgcga agccgtagag caccgaccgg aagcccttgg cgatgttctc
     2341 cgcgccgagc gacggcccga cggtgcgctc ctcgatgatg tccatgggcg ccgcaagcgc
     2401 gcccgcgcgc agcaggagcg cgaggttgcg cgcttcctgc acgtcgccga tgccggaaat
     2461 ctggaagcgg ttgccgaact cctcctggat cacgggccag gtgagcacgg tggtggagtt
     2521 gcgctccacc agcatcatgc ccatgcgctt cttcaggttc tcgcgcgtgg tctggcgcat
     2581 gatgcgcccg ccggtgtcgt cgagcgacat ggagacgacg ggccggccgt cgcgctggtc
     2641 gaagccgggc tgggcatcct tgatgcggtc gccggtgatg acgagcatct tcttcaccag
     2701 caccgcgttg ccctcggtat cgaagaactt gtcggtgccg aagggcgccg gctggtcctt
     2761 gtaggcgatg aaggcgttct gcccgggtcc cgccgcgtgc tcttccgaaa ccaggcgcac
     2821 ttccagcgtt gcggtacggc ccaggatctc cttggcgcgg cccgggtcct gcacgcccgc
     2881 gagctgcacc acgatgcggt ccgcgccctg ctgctggatg atgggttccg ccacgcccag
     2941 ctcgttaacg cggttgcgca gcgtctggat gttctgctgc agggccagct cctgctcctt
     3001 cttcacgacc tcgggcttga tcgtggcgac gatggccgct tcctcgccgg cgtcctgctc
     3061 gcggatcgcg aggtccggca tctccttctc gatcagcttc tgcgcctcga ggcgctggtc
     3121 gcgctcgcgg aagcgcacca ccacgcggtc gccctcgcgc gcgaggcccg agtagtagat
     3181 cttcttgtcg cgcagcaggc tccgcacgtc gcccacgtag cgctcgaccg acttgctggc
     3241 cgccgccttc atgtccacct gcagcaggaa gtgcacgcct ccgcgcaggt ccaggcccag
     3301 gtacatgggc ttcgcgccca gcgcctgcag ccagcccggg gtggacgact ggaggttcag
     3361 cgccacgacg taacccggcg ggaccttcga ctggatgatg tcgcgcgcct tgatctgggt
     3421 gtcggtgtcg gcgaagcgga aggtcgcctg cccctcggaa tcgtccacgc cggccagcgc
     3481 gatgccggcg cccttgatgg cggcttcgat cccggtgcgc atcggctcg
//
LOCUS       DASUEV010000057         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_131801, whole genome shotgun sequence.
ACCESSION   DASUEV010000057 DASUEV010000000
VERSION     DASUEV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131801"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFV55_01825"
     CDS             complement(<1..458)
                     /locus_tag="VFV55_01825"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX4943058.1"
                     /translation="MGNAVVLVSGGLDSATVLAMARAQGWRCHALSVDYGQRHRSELD
                     AAAAVARSLGAVEHRVVRVELGVFGGSALTDTAIAVPTAPSAGIPVTYVPARNTILLS
                     LALAYAEVIEADAIFTGANAVDYSGYPDCRPEYLAAFEAMANLATKRAVEG"
     gene            complement(469..1287)
                     /gene="ybgF"
                     /locus_tag="VFV55_01830"
     CDS             complement(469..1287)
                     /gene="ybgF"
                     /locus_tag="VFV55_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX4943059.1"
                     /translation="MPASSIRLASAAILFAAAGLANAGLFDDDEARRRIEDTRAAQEK
                     VARETAERIGKLEESVRNLGVIDLLRQIEGINAEIARLRGSLEVLANQNEQIQKRQRD
                     FYLDLDSRLKRLEGGAAAPASSLPAAPSAEPVAAASPAATPKDEQAREVRAYDAASSL
                     FRRNDFASAMEAFRAFLKDFPQSTLAPNANYWIGICQANLKDYKGALATQEGLLARYP
                     QSPKAPDALLAIAAVQSEQGDSGSARNTLEDIIARYPGSEAAGKARTRLAAIRH"
     gene            complement(1274..1810)
                     /gene="pal"
                     /locus_tag="VFV55_01835"
     CDS             complement(1274..1810)
                     /gene="pal"
                     /locus_tag="VFV55_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX4943060.1"
                     /translation="MKKIVMSIALVTLVAACSSQEVKKDVPVVDQSTTSTQQPTRPPT
                     TTTTSPTAQPAITANPLTDPKNILSKRSVYFDFDSNVVKDEFRGLVQSHAKYMVDKRD
                     TRIRIEGNCDERGSREYNLALGQRRAESVKKVMTVLGVQEGRVETVSFGEEKPAAAGH
                     DEASWAQNRRADIRYAGE"
     gene            complement(1885..>2130)
                     /locus_tag="VFV55_01840"
     CDS             complement(1885..>2130)
                     /locus_tag="VFV55_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEX4943061.1"
                     /translation="ESGRFRIAVLEIGTGQVTVLTDGTLDDYPSYAPNGKMILYEAQV
                     GGRGQLAAVSSDGRVRQRLVSSAGDVRDPAWGPLPTN"
BASE COUNT          308 a          712 c          754 g          356 t
ORIGIN      
        1 ccctccaccg cccgcttcgt cgcgaggttg gccatggcct cgaaggcggc caggtactcg
       61 gggcggcagt cggggtagcc ggaatagtcg acggcgttcg cgcccgtgaa gatcgcatcg
      121 gcctcgatca cctcggcgta cgcgagggcc agcgacagca ggatggtgtt gcgcgccggg
      181 acgtaggtca cgggaatgcc ggccgagggc gcggtcggca cggcaatcgc ggtatcggtg
      241 agcgcggaac cgccgaacac gccgagttcg acccgcacga cgcggtgctc gacggcgccc
      301 agcgatcgcg ccacggcggc tgccgcatcg agctccgagc ggtggcgctg gccgtagtcc
      361 accgacagcg catggcagcg ccacccttgc gcccgagcca tggcgagcac ggtcgccgaa
      421 tcgaggcccc ctgaaacgag gacgacggca ttccccaccg cgtgccgcct agtgccggat
      481 cgccgcgagg cgcgtgcggg ccttgcccgc ggcttccgag ccggggtaac gggcgatgat
      541 gtcctcgagc gtgttgcgcg cggagccgct gtcgccctgc tcggactgga ccgcggcgat
      601 ggcgaggagc gcgtcgggcg ccttgggcga ctggggataa cgcgcgagca gcccttcctg
      661 cgtggcgagc gcgcccttgt agtccttgag gttcgcctgg cagatgccga tccagtaatt
      721 ggcattcggc gcgagcgtgc tctgcggaaa atccttgagg aaggcgcgga acgcctccat
      781 cgccgaggcg aagtcgttgc ggcggaagag gctggaggcc gcgtcgtatg ccctgacttc
      841 gcgggcctgc tcgtccttcg gcgtcgccgc gggagatgcc gccgcgaccg gctccgcaga
      901 cggtgccgcg ggcaacgacg aagccggcgc ggcagcgccg ccctcgaggc gcttcagtcg
      961 cgagtcgagg tcgaggtaga agtcgcgctg ccgcttctgg atctgctcgt tctggttggc
     1021 cagcacctcg aggctgccac gcaggcgcgc gatctccgca ttgatgcctt cgatctggcg
     1081 aagcaggtcg atgacgccga ggttgcgcac gctctcctcg agcttgccga tgcgctcggc
     1141 agtctcgcgc gcaaccttct cctgtgcggc gcgcgtgtcc tcgatgcgcc tcctcgcctc
     1201 gtcgtcgtcg aaaagaccgg cgttggcaag gcccgcggcg gcgaacagga tcgccgccga
     1261 agccaggcgg atgctactcg ccggcatatc ggatgtcggc ccggcggttc tgggcccacg
     1321 aagcctcgtc gtgcccggct gccgcgggct tctcttcccc gaagctcacg gtctcgacgc
     1381 ggccctcctg cacgcccagc acggtcatca ccttcttcac ggactcggcg cggcgctggc
     1441 ccagggcgag gttgtactcg cggctgccgc gctcgtcgca gttgccctcg atgcggatgc
     1501 gcgtgtcccg cttgtccacc atgtacttgg cgtgcgactg cacgagtccg cgaaattcgt
     1561 ccttcaccac gttggaatcg aagtcgaaat agacgctgcg cttggacagg atgttcttcg
     1621 ggtcggtgag cgggttcgcg gtgatggcgg gctgcgcggt gggcgacgtg gtggtcgtcg
     1681 ttggcggcct cgtcggctgc tgggtcgatg tggtcgactg gtcgacgacg ggaacgtcct
     1741 tcttgacctc ctggctgctg caggcggcga cgagggtgac caacgcaatg ctcatgacga
     1801 ttttcttcac ggatgactcc ttcgaagtga tgcgcacgta aacacgtaat gcggaaagcg
     1861 gaaagcgggg attctgcacc ccgctcaatt cgtaggcaac ggcccccacg cgggatcgcg
     1921 gacgtcgccg gccgacgaca ccaggcgctg ccgcacgcgc ccgtcgctcg acacggcggc
     1981 gagctggccg cgcccgccga cctgcgcctc gtagaggatc atcttgccgt tgggggcgta
     2041 gctcgggtaa tcgtcgagag tgccgtcggt gaggaccgtc acctgcccgg tgccgatctc
     2101 gagcaccgcg atgcggaagc gccccgactc
//
LOCUS       DASUEV010000058         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1384842, whole genome shotgun sequence.
ACCESSION   DASUEV010000058 DASUEV010000000
VERSION     DASUEV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384842"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFV55_01845"
     CDS             <1..607
                     /locus_tag="VFV55_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX4943062.1"
                     /translation="EMRGAMNSIGGISWERLEAQSSVTYPCRTEDDPGQPVVFTDTFP
                     TKDGKAKFVPADIIPANERPDAEYPFVLITGRQLEHWHTGSMTRRASVLDAIEPRAVV
                     SMHPLDLDRIGAVAGDVITVASRRGEIALAARADDGTPPGSVFIPFAFYEAAANLLTN
                     PALDPFGKIAEVKYCAVQVRKGGTLAELASYGGGQSFAPVA"
     gene            691..1662
                     /locus_tag="VFV55_01850"
     CDS             691..1662
                     /locus_tag="VFV55_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4943063.1"
                     /translation="MFRRLAALAAGCLVAVLAPLAQAQTWPAKSVKIIAVFPPGGSVD
                     QVSRIFAAQLTTQLGQQFVVENKGGASGSIGAAAVAQSAPDGYTFGVVFDTHGVNPSL
                     IPNMSFDTRKDLASVMLVGTSPMAIVTHVSQPYKDFRDVLAAAKAKPGSVSFGSIGTG
                     SLGHLAMAQVGNQLGLEFNHIPYRGGGPLMIDAVGGQVPLAIGTVFLVNPHVKGGKVR
                     ALGVTSARASPQMPGVAPIAEQGVPGFSALAWWGVIAPAGTPPAIVRRMHDELAKALK
                     VPAVAEKLTAQGMDIIGGGPEELDRFLKGEIDRWAKVVKDNKIKAGD"
     gene            1772..2653
                     /locus_tag="VFV55_01855"
     CDS             1772..2653
                     /locus_tag="VFV55_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4943064.1"
                     /translation="MAQYRKEGNFEKPAATFNGIFMLAVVVGLAALEIFAVMGVRSPA
                     LIVPLALAIAFVAGGFFMLQPNEAGVLTLFGDYAGTERTPGLRWTWPWLMRRRVSVRA
                     RNHNVDTLKVNDKRGNPVEIAAVVVWRVEDTAQALYDVEDFEHFVKVQSESALRHVAT
                     RYNYDEGEEHGAQEITLRAGADTVAGDLRDELQARVQLAGVKIEEAKLTHLAYAAEIA
                     GAMLRRQQAEAVIAARTKIVAGAVSMVEMALKSLSEKNLVELDDERRAAMVSNLMVVL
                     CSERDVQPVVNAGTLYP"
     gene            2709..2891
                     /locus_tag="VFV55_01860"
     CDS             2709..2891
                     /locus_tag="VFV55_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943065.1"
                     /translation="MASPDKKAFLLRIDPELWAELERLAQAELRSVNGQVEYLLRDAL
                     ERRGMRRTGARKPRKP"
BASE COUNT          479 a         1004 c         1003 g          467 t
ORIGIN      
        1 ggaaatgcgc ggcgcgatga actccatcgg cggcatctcg tgggagcggc tggaggcgca
       61 gtcttcggtc acctatccct gccgcaccga ggacgatccc ggccagcccg tcgtgttcac
      121 cgacacgttc cccacgaagg acggaaaggc gaagttcgtt cccgcggaca tcatccccgc
      181 caacgaacgc cccgacgccg agtatccgtt cgtgctcatc accggccggc agctcgagca
      241 ctggcacacg gggtcgatga cgcgccgtgc ctcggtgctg gatgcgatcg agccccgtgc
      301 cgtcgtctcg atgcacccgc tcgacctcga tcggatcggc gccgtggcgg gtgacgtgat
      361 caccgttgcc tcccgtcgcg gcgaaatcgc gctcgcggcg cgcgccgacg acggcacgcc
      421 gcccggcagt gtcttcattc ccttcgcgtt ctacgaggcg gcggccaacc tgctcaccaa
      481 ccccgcgctc gatccgttcg gcaagatcgc cgaggtgaag tattgcgcgg tgcaggttcg
      541 caagggcgga acgctcgccg aactcgcgag ttatggaggc gggcagtcct tcgcgccggt
      601 cgcctgaaga cgtctttgcg cagccgtatc cgtggtcgat ccgcttcccg gatcgaccgg
      661 atccatccag aagagaggta ttccgtgcac atgttccgtc gtctcgccgc gctggccgcg
      721 ggttgtctcg tcgccgtcct ggcgccgctc gcgcaggccc agacctggcc cgccaagtcc
      781 gtgaagatca tcgccgtgtt cccgcccggc ggctccgtcg accaggtttc gcgcatcttc
      841 gccgcgcagc tcaccacgca gctcggccag cagttcgtgg tcgagaacaa gggcggcgca
      901 tccggctcga tcggcgcggc ggccgtcgcg cagtcagctc ctgacggcta caccttcggc
      961 gtggtgttcg acacccacgg ggtgaatccc tcgctcatcc cgaacatgtc cttcgacacg
     1021 cggaaggatc tcgcctcggt gatgctcgtg ggcacctccc ccatggcgat cgtgacccac
     1081 gtgagccagc cctacaagga tttccgcgac gtcctggctg ctgccaaggc gaagccggga
     1141 agcgtgagct tcggctccat cggtaccggc agcctgggcc acctcgcgat ggcgcaggtc
     1201 ggcaaccagc tgggcctcga gttcaaccac atcccctatc gcggcggcgg cccgctcatg
     1261 atcgatgcag tcggcggaca ggtgcctttg gccatcggca cggtgtttct cgtgaacccg
     1321 cacgtgaagg gcggcaaggt gagggcgctg ggcgtgacct ccgccagggc ctccccgcag
     1381 atgcccgggg tggcgcccat cgcggagcag ggcgtgcccg gattctcggc gctcgcctgg
     1441 tggggcgtga tcgccccggc cggcacgccg cccgccatcg tcaggcgcat gcacgacgag
     1501 ctcgccaagg cgctcaaggt gcccgccgtc gcggaaaagc tcaccgcgca gggcatggac
     1561 atcatcggcg gcggacccga ggagctcgac aggttcctga agggcgagat cgaccgctgg
     1621 gcgaaggtgg tcaaggacaa caagatcaag gcaggcgatt gatgccgcga cactaggcgt
     1681 cccccggccg gcgaagccgg cgtgcttgac agcccggcga attcctatca aagtgatatc
     1741 actttaatag ctccccgccg ggagggacgc catggcccag tatcgcaagg aaggcaattt
     1801 cgagaagccc gccgccacct tcaacggcat cttcatgctc gcggtcgtcg tggggctcgc
     1861 cgcgctcgag atcttcgccg tgatgggcgt gcgctcgccc gcgctgatcg tgccgctggc
     1921 gctcgcgatc gcgttcgtgg cgggcggctt cttcatgctg cagcccaatg aagcgggcgt
     1981 gctcacgctg ttcggcgatt acgccggcac cgagcgcacg ccgggcctgc gctggacctg
     2041 gccctggctc atgcgcagga gggtgagcgt gcgcgcgcgc aaccacaacg tggacaccct
     2101 caaggtgaac gacaagcgcg gcaatcccgt cgagatcgcc gcggtggtcg tctggcgggt
     2161 ggaggacacg gcccaggcgc tctacgacgt cgaggatttc gagcatttcg tcaaggtgca
     2221 gagcgagtcg gcattgcgcc acgtggccac gcgctacaac tacgacgagg gcgaggagca
     2281 cggggcgcag gaaatcacgc tgcgggcggg cgccgacacg gtggccgggg acctgcgcga
     2341 tgaactgcag gcccgcgtcc agttggccgg ggtgaagatc gaggaggcga agctcaccca
     2401 ccttgcctac gccgccgaga tcgccggcgc gatgttgcgc cgccagcagg cggaggccgt
     2461 gatcgccgcg cgcaccaaga tcgtcgcggg cgcggtctcg atggtcgaga tggccctgaa
     2521 gagcctttcc gagaagaacc tcgtggagct ggacgacgag cgccgcgccg ccatggtctc
     2581 gaacctcatg gtcgttctct gctcggagcg cgacgtccag ccggtcgtga acgccggtac
     2641 gctctacccg taggcgcgcg atggagtccg cagcgccggc ggcgccgagg cagtctgcgt
     2701 aggcgacgat ggcaagcccc gacaagaagg cgttcctgct gcgcatcgat ccggagctct
     2761 gggccgagtt ggagcgcctc gcccaggcgg agctgcgcag cgtgaacggc caggtcgagt
     2821 acctgctgcg cgacgcgctc gaaaggcgcg gcatgcgccg cacaggcgcg aggaagccgc
     2881 gcaagcccta gcgatgcccc gtctttcgaa tcttcgccga attgcgggga tgcgaccgta
     2941 gtgcctgata tgc
//
LOCUS       DASUEV010000059         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1154275, whole genome shotgun sequence.
ACCESSION   DASUEV010000059 DASUEV010000000
VERSION     DASUEV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154275"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFV55_01865"
     CDS             complement(<1..239)
                     /locus_tag="VFV55_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX4943066.1"
                     /translation="MITRREMLGLALAAAAVAGCDRLFPGRVPFNGVDITGSDLGPDF
                     RLTDHNGKERTLADFRGKAVALFFGYTYCPDVCPTT"
     gene            complement(239..1138)
                     /gene="cyoE"
                     /locus_tag="VFV55_01870"
     CDS             complement(239..1138)
                     /gene="cyoE"
                     /locus_tag="VFV55_01870"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX4943067.1"
                     /translation="MSAHAMQGAADVLGQFWRLTKPRVVSLIVFTAVIGMLLAAPGLP
                     PAVPMFYGTVGIALVAGAAAAFNCLVEQKLDAVMARTRGRPTVMGSITTAQVLLMSGA
                     VGGLGLAILWHAVNPLTMWLTLATFVGYAIVYTVVLKPATPQNIVIGGASGAMPPVLG
                     WAAISGDVTYQPLLLFLIIFAWTPPHFWALALYRRREYERAGIPMLPVTHGEAYTRLH
                     LLLYTLIMVATTLLPFATGMAGLFYLGSALVLDAVFLVYAVAIYVSYTDSLAKRTFRY
                     SIFYLAMLFAALMIDHYLQLLPA"
     gene            complement(1254..1814)
                     /locus_tag="VFV55_01875"
     CDS             complement(1254..1814)
                     /locus_tag="VFV55_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX4943068.1"
                     /translation="MISRPRLKLVAIMALFALPIVASTVAYRFFRPEATANYGELLVP
                     PAPITTAQFLRAGGGTFRFEELRGKWVLIASDSGACPASCTAKLTMMRQVRLMLGRNA
                     ARVVRVFVADDTHTLEPGALAPYEGTVAITPPPGTALPSAPVNDRAHFYLADPLGNVM
                     MRFPEGAEPRPMLRDLERLLKASQIG"
     gene            complement(1811..2521)
                     /locus_tag="VFV55_01880"
     CDS             complement(1811..2521)
                     /locus_tag="VFV55_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX4943069.1"
                     /translation="MRIAGFVFAPRVVPTLAAVAMVALTVSLGRWQADRAQEKAGRQA
                     LLEARVAEAPARLTGMARSAEDLLFRRVRAEGRFVPTGQIFIDNRIHGGRAGFHVVTP
                     LAIGGAGPLVLVIRGWVARSVAYPAPPDVPVPAGEQVVEGLATVPPARVLELSTETVA
                     GNVWQNLSIARYAKLAGRDVLPVMILASPPAPGLVAVEEKPDAGILKHREYSLTWFSL
                     AATVVALWVGVNLRRDRP"
     gene            2588..2785
                     /locus_tag="VFV55_01885"
     CDS             2588..2785
                     /locus_tag="VFV55_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX4943070.1"
                     /translation="MKIVVILLLLAIIASLFSGLFFVYKDKGESNRAVVSLTIRIVLS
                     LLVFLILIGSYFFGLVPGGPG"
     gene            complement(2974..>3687)
                     /locus_tag="VFV55_01890"
     CDS             complement(2974..>3687)
                     /locus_tag="VFV55_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX4943071.1"
                     /translation="GIGAAVLVYMLFGWFGTVIRESEGGLFNKQVDMSFRWSMSWFIF
                     SEVMFFAAFFGALFYARVYSLPWLGDPDNKLLWPEFSARWPSAGPYRPPQFSPMHAWG
                     VPALNTFLLLSSGVTVTFAHWALKVGNRKGLILGLLATVVLGFSFLGFQIYEYVHAYA
                     ELNLKLTTGVFGSTFYMLTGFHGFHVTLGAVMLTVILVRSIKGHFTADHHFAFEAVAW
                     YWHFVDVVWLLLFVFVYWV"
BASE COUNT          682 a         1218 c         1248 g          539 t
ORIGIN      
        1 gtcgtggggc agacgtccgg gcaatacgtg tagccgaaga agagcgcgac cgccttgccg
       61 cgaaagtccg ccagcgtgcg ctccttgccg ttgtggtccg tcaggcggaa atcggggccg
      121 aggtcggagc cggtgatgtc cacgccgttg aacggcaccc gccccggaaa cagcctgtcg
      181 caacccgcca ccgcggccgc ggccagagcc aggccgagca tctcgcggcg agtgatcatc
      241 atgccggcag cagttgcagg tagtggtcga tcatcaacgc cgcgaagagc atcgcgaggt
      301 agaagatcga gtacctgaac gtgcgcttgg caagcgagtc cgtgtagctc acgtagatcg
      361 ccacggcgta gacgaggaag accgcatcca gcaccagggc agagcccagg tagaagagcc
      421 ccgccattcc ggtcgcgaag ggcagcagcg tcgtggccac catgatcagc gtatagagca
      481 gcaggtgcag cctcgtgtac gcctcgccgt gcgtgaccgg gagcatgggg atgccggcgc
      541 gctcgtactc gcggcggcga tagagcgcga gcgcccagaa gtgcggcggc gtccacgcga
      601 agatgatgag gaagagcaga agcggctggt aggtgacatc cccgctgatc gcggcccacc
      661 ccagcacggg cggcatcgcc cccgaggccc cgccgatcac gatgttctgc ggcgtggcag
      721 gcttcaggac caccgtgtag acgatcgcat agcccacgaa tgtggccagc gtcagccaca
      781 tggtgagcgg gttgaccgcg tgccacagga tggcgagccc gagaccgccg acggcgccgg
      841 acatgaggag cacctgcgcc gtggtgatcg agcccatcac cgtgggccgc ccgcgcgtgc
      901 gcgccatcac ggcatcgagc ttttgctcga cgaggcagtt gaacgcggcc gcggctccgg
      961 ccacgagcgc gatgcccacg gtgccgtaga acatcggaac ggcgggcggg agcccgggtg
     1021 ccgcgagcag catgccgatc accgccgtga acacgatgag cgacacgacg cgcggcttgg
     1081 tgaggcgcca gaactggccg agcacgtcgg ccgcgccctg catcgcatgt gcgctcatcg
     1141 gtgaatttct cgtgtcatgc attccagtgg cctgtagtcg cccgccgcgc cgagccggga
     1201 attataccgt tgcccgcctc gtttcccggg tcccgcggga aggaaatcgc cgctcatccg
     1261 atctgggaag ccttcagcag cctctcgagg tccctgagca tcgggcgcgg ttccgccccc
     1321 tcggggaatc gcatcatcac gtttcccagc ggatcggcga ggtagaaatg cgcgcggtcg
     1381 ttcaccgggg cggagggcag cgccgtgccc ggcggcggcg tgatcgcaac ggtcccttcg
     1441 tagggcgcca gcgcccccgg ctccagcgtg tgcgtgtcgt cggcgacgaa gacccggacg
     1501 acgcgggccg cgttgcgccc gagcatgagg cgaacctgcc gcatcatcgt gagcttggcc
     1561 gtgcacgatg ccgggcacgc ccccgaatcc gaggcgatga gcacccactt gccgcgcagt
     1621 tcctcgaagc ggaaggtgcc gccgcccgcg cgcaggaact gcgccgtggt tatcggggcc
     1681 ggtggcacca ggagctcgcc gtagttcgcc gtcgcctccg gccggaagaa gcgataggcg
     1741 accgtggacg ccacgatggg cagcgcgaag agcgccatga tcgccacgag cttcaggcgc
     1801 gggcggctga tcatggacgg tccctgcgca ggttgacgcc cacccatagc gccaccaccg
     1861 tcgcggcgag cgagaaccac gtgagcgaat attcgcgatg cttgaggatt cccgcgtcgg
     1921 gcttctcttc gaccgcgacc agccccgggg cgggcgggga ggcaaggatc atcaccggca
     1981 gcacgtcgcg cccggcgagc ttcgcgtagc gcgcgatcga gaggttctgc cacacattac
     2041 cggcgaccgt ttccgtggag agttccagca cgcgggccgg cggcacggtg gcgagcccct
     2101 ccaccacctg ctcgcccgcc ggaactggaa cgtccggcgg cgccggatag gcgaccgaac
     2161 gggcgaccca gccgcggatc acgaggacaa gcggcccggc gcccccgatc gcgagcgggg
     2221 tcaccacatg gaatcccgcg cggccgccgt ggatgcggtt gtcgatgaag atctggcccg
     2281 tgggaacgaa tcgtccctcg gcgcgaacgc gccggaagag gagatcctcc gcggaacggg
     2341 ccatgccggt gagcctggcc ggcgcctcgg caacgcgagc ctcgagaagc gcctggcgac
     2401 cggccttctc ctgcgcgcgg tccgcctgcc accgcccgag cgaaaccgtc agcgcgacca
     2461 tggccactgc cgccaaggtc ggcacgacgc gcggcgcgaa gacgaagccc gcgatcctca
     2521 tcggccggcc gcttccggtg gggcgtaaaa tacgcggtcg aaagccgttc ccgcgcgcaa
     2581 tccccccatg aaaatcgtcg tcatcctgct gcttctcgcc atcatcgcga gcctgttctc
     2641 cggcctgttc ttcgtctaca aggacaaggg ggaatcgaac cgcgcggtgg tctcgctcac
     2701 gatccgcatc gttctctccc tcctcgtgtt tctgatcctg atcggatcgt acttcttcgg
     2761 cctggtgccg ggcgggccag ggtagaaaag gggtcaggtt agtttgcaca aaaaataggc
     2821 aagacggaaa aaggggtcag gttacttttc tgtcgaattg ccgtcgctag acggcgggct
     2881 gaaaagtaac ctgacccctt tttccgtctt gcctattttt tgtgcaaact aacctgaccc
     2941 cttttctacc ccggcccgcg gcgcggtcga cgcctagacc cagtagacga agacgaagag
     3001 cagcagccag accacgtcca cgaagtgcca gtaccaggcc accgcctcga aggcgaagtg
     3061 gtggtcggcg gtgaagtggc ccttgatcga ccgcaccagg atcaccgtga gcatcaccgc
     3121 gcccagcgtc acgtgaaagc cgtggaagcc ggtgagcatg tagaacgtgg agccgaacac
     3181 acccgtggtg agcttgaggt tgagttcggc gtaggcgtgg acgtactcgt agatctggaa
     3241 gccgaggaac gagaagccca gcaccacggt ggcgaggagc cccaggatca gtcccttgcg
     3301 gttgcccacc ttgagcgccc agtgcgcgaa ggtgaccgtc acgcccgagg acagcagcag
     3361 gaacgtgttg agcgccggga ccccccaggc gtgcatcggc gagaattggg gcggccggta
     3421 cgggccggcg gagggccagc gggcgctgaa ctccggccac aggagcttgt tgtccgggtc
     3481 gccgagccac ggcagcgagt acacgcgcgc gtagaagagc gcgccgaaga acgcggcgaa
     3541 gaacatcacc tccgagaaga tgaaccagct catgctccag cggaaggaca tgtcgacctg
     3601 cttgttgaac agcccgccct cgctttcgcg gatcaccgtg ccgaaccacc cgaagagcat
     3661 gtagacgagg accgccgcgc cgattcc
//
LOCUS       DASUEV010000060         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1154535, whole genome shotgun sequence.
ACCESSION   DASUEV010000060 DASUEV010000000
VERSION     DASUEV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154535"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..820)
                     /locus_tag="VFV55_01895"
     CDS             complement(47..820)
                     /locus_tag="VFV55_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX4943072.1"
                     /translation="MNLDDDLLQAFVDGALNASERATVEAALAQDADANRRALRFMEQ
                     RRLLHEQFDPVLLEPVPERLHMRRPPWLDYARAASVLVVGVAIGLAMPYAWKGGRLAT
                     PEVASAPAVAPVPAALAMRAARAHLVYASEVRHPVEVDASQQDHLVAWLSKRLGTKLK
                     VPVLGEEGFELLGGRLLPGPDGPVAQFMYQESSGKRLTLYVSARAKNEGQTAFRFARE
                     DGVSVFYWIDGNWGYALSGEIDKSALSRVSTSVYRQLNP"
     gene            complement(817..1308)
                     /locus_tag="VFV55_01900"
     CDS             complement(817..1308)
                     /locus_tag="VFV55_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4943073.1"
                     /translation="MDYELVVPFIPNLRRYARALVGDRHGADDLVQDTLERAVRKFHL
                     WRPGDMRAWLFSIMHNVFVNQLKARKISSSVELDESIAGATTPGWVTDVRDLERGLAL
                     LPADQREVVLMVSLEDMSYQEVSRALGVPIGTVMSRLSRGREKLRRHMDGESTRTNLK
                     VVL"
     gene            complement(1319..1681)
                     /locus_tag="VFV55_01905"
     CDS             complement(1319..1681)
                     /locus_tag="VFV55_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943074.1"
                     /translation="MRTLLLATAFALPAMASAQAPTKTAGGVLTNAAGMTLYTFDKDE
                     AGKGKSACNGPCATNWPPLMAAADAKATGDYSIVTRDDGGKQWAFKGKPLYGWAKDQK
                     PGDKTGDGVNKVWHVATP"
     gene            complement(1824..2453)
                     /locus_tag="VFV55_01910"
     CDS             complement(1824..2453)
                     /locus_tag="VFV55_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4943075.1"
                     /translation="MNVTLYYSPGACSLAPHIVLEELGLAYGTVRISTAEGQQRSPEY
                     LKINPRGRVPALVVDGKVLVENVAILTFLGGGFAARGLWPEQTWDQAQALSLMAWLAD
                     TVHPAFAHFYRPERYVEGSRCVDAVKAAGRESFGQHLAEIDRLVAGKKWAVGNRFSVL
                     DAYLLVFYRWGNRNGFAVQGLASYAALVGRILARPAVKKVMADEGITMT"
BASE COUNT          404 a          842 c          876 g          419 t
ORIGIN      
        1 gaaccaggtc acatttccgg cgtcgccgcc ggacgcccgc gcccgctcag ggattgagct
       61 ggcggtagac gctcgtcgaa acgcgggaga gcgcggattt gtcgatctcg ccggacagcg
      121 cgtagcccca gttgccgtcg atccagtaga acaccgacac gccgtcttcc ctggcgaagc
      181 ggaaggcggt ctggccctcg ttcttggcgc gggcggagac gtagagcgtg aggcgcttgc
      241 cgctcgactc ctggtacatg aattgcgcga ccggaccatc gggcccgggc agcaggcgcc
      301 cgccgagcag ctcgaagccc tcctcgccga gcaccggcac cttgagcttc gtgccaagcc
      361 gcttggagag ccaggccacg aggtggtcct gctgggaggc gtcgacttcc accgggtggc
      421 gcacttcgga tgcatacacg aggtgggctc gggccgcccg catcgccagg gcagcaggta
      481 ccggcgccac cgcgggcgcg gatgccacct cgggcgtcgc aaggcggccg cccttccacg
      541 cgtagggcat tgccagcccg atggcgacgc ccacgacgag caccgacgcg gcccgcgcat
      601 agtcgagcca cggcggacgg cgcatgtgca ggcgctcagg aacgggctcg agcagcacgg
      661 ggtcgaactg ctcgtggaga aggcggcgct gctccatgaa gcgcagcgca cgccggttgg
      721 catcggcatc ctgcgcgagc gccgcttcca cggtagcccg ctcggaggcg ttcagggcgc
      781 cgtccacgaa tgcctgcaac agatcgtcgt ctaggttcac agcaccacct tgaggttcgt
      841 gcgcgtcgat tcgccgtcca tgtggcggcg cagcttctcg cgcccgcgcg acaggcgcga
      901 catcaccgtc ccgatgggca cgcccagcgc gcggctcact tcctggtaac tcatgtcctc
      961 gaggctcacc atgagcacga cctcgcgctg gtcggcgggc aggagggcga gcccgcgctc
     1021 gaggtcgcgc acgtccgtga cccagccggg cgtggtcgcg cccgcgatgg attcgtccag
     1081 ctccacggaa gacgagatct tcctcgcctt cagctggttc acgaacacgt tgtgcatgat
     1141 cgagaagagc caggcgcgca tgtccccggg ccgccacagg tgaaacttgc gcaccgcgcg
     1201 ctcgagggtg tcctggacga ggtcgtcggc cccatggcgg tcccccacca gcgcgcgcgc
     1261 gtagcggcgc aggttgggga tgaacgggac gacgagctcg tagtccatgg gacgtcggtc
     1321 agggcgtggc tacgtgccag accttgttca cgccgtcgcc cgtcttgtcg ccgggcttct
     1381 ggtccttggc ccagccgtag aggggcttgc ccttgaacgc ccattgcttg ccgccgtcgt
     1441 cgcgcgtgac gatcgaataa tcgccggtgg ccttggcatc ggccgccgcc atcaacgggg
     1501 gccagttcgt ggcgcagggg ccgttgcagg cgctcttgcc cttgccggcc tcgtccttgt
     1561 cgaacgtgta gagcgtcatg ccggcggcgt tggtaaggac gccgcctgcg gtcttggtgg
     1621 gtgcctgcgc cgatgccatt gcgggcaggg caaaggcggt ggcgaggagt agcgtgcgca
     1681 gaatcttcat tggtatggtc cagagtggtt tgggggtcct acgaacggat gaacacgccc
     1741 gttcgccgtt ttattccgtg ccgccggcgc cagccccgct tcgagcgtac gctcgacagc
     1801 cgccaggcgg ccacgccccg tgctcaggtc atggtgatgc cttcgtcggc catgaccttc
     1861 ttcacggcgg gccgggcaag gatgcgcccg acgagcgccg catagctcgc tagtccctgc
     1921 actgcgaagc cgttgcggtt tccccagcgg tagaagacga gcaggtaggc atccagcacc
     1981 gagaagcgat tgccgacggc ccacttcttc ccggcgacaa ggcggtcgat ttccgccagg
     2041 tgctggccga aggactcgcg cccggccgct ttcaccgcgt cgacgcaccg ggaaccttcc
     2101 acgtagcgtt cgggccggta gaagtgcgcg aaggcgggat ggacggtatc ggcgagccag
     2161 gccatgagcg agagcgcctg cgcctggtcc caggtctgct ccggccacag gcccctggcg
     2221 gcgaagccgc ctccgagaaa ggtgaggatg gccacgttct ccaccagcac cttgccgtcg
     2281 accaccagcg ccggcacgcg accgcgcggg ttgatcttca ggtattcggg cgagcgctgc
     2341 tgcccctcgg cggtgctgat cctcaccgtc ccgtaggcga gacccagctc ctcgaggacg
     2401 atgtgcggcg cgagcgagca ggcgcccggg gagtagtaga gcgtgacgtt cacgaatttc
     2461 tccggggtga aaaggggtca ggttactttg cacaaaaaat aggcaaatcg gaaaaggggt
     2521 caggttactt ttctgttgta t
//
LOCUS       DASUEV010000061         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1507082, whole genome shotgun sequence.
ACCESSION   DASUEV010000061 DASUEV010000000
VERSION     DASUEV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507082"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFV55_01915"
     CDS             <1..1200
                     /locus_tag="VFV55_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943076.1"
                     /translation="SVQDSTRADDIQAYLDKYPKGVFAPLARNRIDALLGRNRVAAAA
                     APAEAARSVPSVPAPSSPAAAPSTTVPDQVAATSPPASAKPAAAPKAGDTSAGERPAV
                     VAVIAPPAAASTTPAQGAAAPVTSAAPAPKPRDDQPGREIAPGIREVTFADGAVYVGA
                     MRGIQLHGKGQYTSKSFKYQGEFKDGVKHGRGSYVWENGDRYEGDFAEDRPNGKGKYQ
                     FANGDAYEGDVKAGVITGRGVYVAKSGDRIEGSFIDGRASGSGIYRFASGDRYEGEMT
                     GGKLQGKGRYFAKSGDRIEAPFVDGKAQGQGVYHFSNGDRYEGDLREGALTGTGAYFY
                     ASGQKYEGEVAHGRPQGKGTFWFADGSRFEGVFDNGLARAKGAVVKADGTRAAAEIID
                     GAVRLVN"
     gene            1271..1399
                     /locus_tag="VFV55_01920"
     CDS             1271..1399
                     /locus_tag="VFV55_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943077.1"
                     /translation="MAERKRTRRNTLERRCLSKAFKRLFHGASKSTFKVLEQIKKN"
     gene            complement(1478..>3645)
                     /gene="pepN"
                     /locus_tag="VFV55_01925"
     CDS             complement(1478..>3645)
                     /gene="pepN"
                     /locus_tag="VFV55_01925"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX4943078.1"
                     /translation="IRGNDGAARHWARWEDPFPKPCYLFAMVAAKLDLIEDHFVTKSG
                     RNALLQVYVEPGKLDQAGFALQCLKRAMRWDEERYGLELDLERFMIVAVGDFNMGAME
                     NKGLNVFNTKFVLARADTATDEDFMMIDRVVAHEYFHNWTGNRVTCRDWFQLSLKEGL
                     TVFRDQEYGGDEYSRAVSRIRDVRDLRSRQFPEDAGPMAHPVRPESYVEINNFYTATV
                     YGKGAEVVRMYQTLLGRDGFRRGMDLYFERHDGQAVTCDDFRAAMSDANGADLAQFGL
                     WYSQAGTPVLESHGRYDAGARTYTLAIRQSCPPTPGQAQKQPFHIPLAVGLVDRKGDD
                     MALRLDGETTALSIPDPGTGAITRVIPLTQAETTLVFRDVAEPPVPSILRGYSAPVVL
                     RHEYGDADLTHLMANDADPFNRWEAGQVLATRILLAGVESIRAGKEMVVPPAFVEALG
                     HVLADGARDPAFAAECLQLPGEGVLAEQMAVADPDAIHSARKHLVLEVAQRYRTRFEG
                     AFRHFSVQGTYSPDAAASGRRALRNAALAYIMAIDDSTARALAFLEFRRAENMTDAMA
                     ALSCLADSACAERERALAMFHDRWKDEALVVDKWFRVQAVSGLPGAAGRVEKLLAHPA
                     FDLRNPNRARSVLHAFATDNPVHFHAADGSGYRLIAERLVGLDRLNPQVASRLARAFD
                     RWRKYDAGRQAQAKAALESIRDSGGISADVAEVVNRALD"
BASE COUNT          616 a         1198 c         1287 g          544 t
ORIGIN      
        1 agcgtgcagg attcgacgcg cgccgatgac atccaggcgt atctcgacaa gtaccccaag
       61 ggggtcttcg ccccgctggc ccgcaaccgg atcgatgccc tcctcgggcg caatcgcgtg
      121 gcggcagcgg ccgcgcccgc cgaggctgca cggtcggtac cctcggtgcc cgcaccctcg
      181 agtcctgcgg cagccccttc gacaacggtt cccgaccagg ttgctgccac ctcgcctccc
      241 gcaagcgcga agcccgctgc agcccccaag gctggcgata cgtctgcagg agaaaggccc
      301 gcggtcgtgg ccgtgatcgc accgcctgcg gcagcctcga cgacgccggc ccagggcgcc
      361 gcggcgccgg tgacgtccgc ggcgcccgcg ccgaaaccca gggacgatca gcccggccgc
      421 gagatcgcgc caggcatccg cgaggtcacg ttcgccgatg gggcggtcta cgtgggcgcg
      481 atgcgcggca tccagttgca cggcaagggg cagtacacgt cgaagtcgtt caagtaccag
      541 ggcgagttca aggacggcgt gaagcacggc cgcggctcgt acgtctggga gaacggcgac
      601 cggtacgagg gcgatttcgc cgaggatcgg ccgaacggca agggcaagta ccagttcgcg
      661 aacggcgacg cctacgaagg tgacgtgaaa gccggcgtga tcaccggccg gggcgtctat
      721 gtcgcgaagt caggagaccg aatcgagggt tcgttcatcg atggccgcgc aagcggctcc
      781 ggcatctacc gcttcgcaag cggcgatcgt tacgagggcg agatgacggg cggaaagctc
      841 cagggcaagg ggcgctactt cgcgaagagc ggcgaccgca tcgaggcccc gttcgtggac
      901 ggaaaggcgc aaggccaagg cgtgtaccac ttctcgaatg gcgaccgcta cgagggcgac
      961 ctgcgggaag gcgcgcttac gggcacggga gcgtacttct acgcgtccgg ccagaagtac
     1021 gagggcgagg tggcccacgg ccgcccgcag ggcaagggaa cgttctggtt tgccgacggc
     1081 tcccggttcg agggggtctt cgacaacgga ttggcccggg cgaagggcgc ggtggtcaag
     1141 gcggacggga cgcgtgccgc agcggaaatc atcgatggcg cggtacggct cgtgaactga
     1201 cgaccgccgt cggcgcgcgc ggcgcgacac gctgaggtat aattaacggt tccgcaacaa
     1261 ggagctggac atggccgaac gcaaacgtac ccgccgcaac accctcgagc gccgctgcct
     1321 gtcgaaggcc ttcaagcgcc tgttccatgg cgcctcgaag tcgacgttca aggtgctgga
     1381 gcagatcaag aagaactgac cgcccgggcg gatcgagatt ctcccgacag ggccgcgaaa
     1441 gcggcccttg tcgcttgcgc cgggagccat gcggcgttca gtcgagcgct cggttcacca
     1501 cttccgcgac atcggccgag atgccgcccg aatcccggat cgattcgagg gcagccttcg
     1561 cctgcgcctg ccttccggcg tcgtacttcc tccagcggtc gaaggcgcgc gcaaggcgcg
     1621 acgcaacctg ggggttcagc cggtccagcc ccaccagtct ttccgcgatg aggcgatagc
     1681 cggacccgtc cgcggcatgg aaatgcacgg gattgtccgt ggcaaaggcg tgaagcaccg
     1741 agcgggcacg gttcgggttg cgcaggtcga aagccgggtg cgcgaggagc ttctcgacgc
     1801 ggccggccgc cccgggcagg ccggacaccg cctgcacgcg gaaccacttg tcgaccacga
     1861 gcgcctcgtc cttccaccgg tcgtggaaca tcgccagcgc ccgctcacgt tcggcgcacg
     1921 ccgaatcggc gaggcaggac agggcggcca tcgcgtcggt catgttctcc gcccggcgga
     1981 actcgaggaa ggcgagcgca cgagctgtcg agtcgtcgat cgccatgatg taggcgagag
     2041 cggcattgcg aagcgcgcgg cgcccggaag cggccgcgtc cggcgagtag gtgccctgca
     2101 ccgagaagtg gcggaaggcg ccctcgaacc gggtacggta gcgctgcgcc acctcgagca
     2161 cgaggtgctt tctcgcggag tggatcgcgt cgggatccgc gaccgccatc tgctcggcca
     2221 gcacgccttc gccgggaagc tggaggcact cggcggcgaa cgcggggtcg cgggcgccgt
     2281 cggcgagcac gtggcccaac gcctcgacaa aggccggggg gacgaccatt tccttccccg
     2341 cccggatcga ctccacgccc gcgagcagga tgcgcgtggc aagcacctgc ccggcttccc
     2401 agcggttgaa cgggtcggca tcgttcgcca tcaggtgggt gaggtctgca tcgccgtatt
     2461 cgtggcgcag gacgaccggt gccgagtaac cgcgaaggat cgacggaacc ggaggctcgg
     2521 cgacgtcgcg gaagacgagc gtggtttccg cctgggtcag cggaatcacg cgcgtgattg
     2581 cgccggttcc gggatccggg atcgagagcg cggtcgtctc gccgtcgagg cggagcgcca
     2641 tgtcgtcgcc cttccggtcc acgagaccca cggcgagcgg gatgtggaac ggttgcttct
     2701 gcgcctgccc gggagtgggc gggcaggact gccggatggc cagcgtgtag gtgcgcgcgc
     2761 cggcgtcgta tcggccgtgg ctctccagca ccggcgtgcc tgcttgcgag taccagaggc
     2821 cgaactgggc gaggtcggcg ccattcgcgt cggacatcgc ggcacggaaa tcgtcgcagg
     2881 tgaccgcctg tccgtcgtgg cgctcgaagt acaggtccat ccccctgcga aagccgtcgc
     2941 gcccaagcag cgtctggtac atccgaacca cctcggcacc ctttccgtaa acggtggcgg
     3001 tatagaagtt gttgatctcg acgtaggatt cggggcgcac cgggtgagcc atggggcccg
     3061 cgtcttcggg gaactggcgc gagcgaaggt cgcgcacgtc ccggatgcgc gacaccgcgc
     3121 gcgagtactc gtcgccgcca tactcctggt cacggaagac ggtgaggccc tccttgagcg
     3181 aaagctggaa ccagtcgcgg caggtgacgc ggttgccggt ccagttgtgg aagtactcgt
     3241 gcgcgaccac gcggtcgatc atcatgaagt cctcgtcggt cgcggtgtcg gcgcgcgcca
     3301 ggacgaactt cgtgttgaag acgttgaggc ccttgttctc catggccccc atgttgaagt
     3361 cgcccaccgc gacgatcatg aaacgctcga ggtcgagctc gagcccgtag cgctcctcgt
     3421 cccagcgcat ggcgcgcttc aggcactgca gggcgaagcc ggcctggtcg agcttgcccg
     3481 gctccacgta cacctggagc agggcgttcc ttcccgattt cgtgacgaag tggtcctcga
     3541 tcaggtccag cttcgccgct accatcgcga agaggtagca gggcttcggg aacgggtctt
     3601 cccagcgcgc ccaatgccgc gcggcgccgt cattcccgcg tatgc
//
LOCUS       DASUEV010000062         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_476560, whole genome shotgun sequence.
ACCESSION   DASUEV010000062 DASUEV010000000
VERSION     DASUEV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476560"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2283)
                     /locus_tag="VFV55_01930"
     CDS             complement(<1..2283)
                     /locus_tag="VFV55_01930"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943079.1"
                     /translation="MQRHVPNQTFSDNRARTLAGASFLIVAFAAYCGAAKAQAPAATL
                     PSTGTIAVPTYEAAGLYWQNPGGSNGCEVKFRKAGEASWSQGLPLWYDARDSQCRGSL
                     VSLAPNTYYQVEFNLPGQAATHGLVFKTWSNQLPVAKTIAVNAGSATLNVAEGGSAST
                     GYVVYEGNGATLDGQNSAQYNVTINASYVIVRGLNLKGAKQDAIRISPNVTDVVIEDN
                     DISGWGRQRSGNWGVDMDSAIRAVCTQPTLMRVTIQRNKMHDPRYSANSWSDGHPAGP
                     QAISFSHCGGNHVFRHNEAYSTNGNYFNDIFGGEDNFTKAGFPNSDTDIYGNKLSHAW
                     DDGIESEGANENVRIWGNYIDRTAIGVATTATSIGPVYIFRNVWNRAQMYEKAALDAD
                     DRQPFFKSGGDNTLGHGRRYIFHNTMLQARQSGLQYGLGGGFGLGGTGSAQLIENTFS
                     RNNIYHQWKEGKGFTYQTGSSSSFVSDMYNGTAGDATVTSGIVGTPVYAAGHGWQSEA
                     GGNYQLDASSPGYGRGARIPNFNDGAATPDVGAHQSGAPAMKFGIAASSGSSISGGAT
                     VPTETTPPPPPPTTTQYALTVVGKGNGAGTVISSPSAINCGSTCATSFAAGTTVTLTA
                     QPSPGSTFGGWSGACSGTGSCSLAMTADRSVTATFKKSAGTATSGSGVTLSSTALGDF
                     GTSRTQTVTYTNNTGSKVTFIQASMSSARYGQANNCGEVAPGGSCTATITYYPTNSGS
                     DTGTFTMTSTAPNSPHVVSLS"
BASE COUNT          355 a          779 c          917 g          481 t
ORIGIN      
        1 gctcaggctg accacgtgcg ggctgttcgg tgccgtgctg gtcatggtga acgtgccggt
       61 gtccgagccc gagttggtcg ggtagtaggt gatggtcgcc gtgcagctgc cgccgggcgc
      121 gacctcgccg cagttgttcg cctggccgta gcgcgcgctc gacatcgagg cctggatgaa
      181 ggtgaccttg gaacccgtgt tgttggtgta ggtgacggtc tgcgtgcggc tcgtgccgaa
      241 atcccccagc gcggtgctac tcagcgtcac cccggatccc gacgtcgcgg tgccggcgga
      301 cttcttgaag gtcgcggtga cgctgcggtc ggccgtcatg gcgagcgagc aggagcccgt
      361 cccgctgcag gcgcccgacc agccgccgaa cgtcgagccc ggcgagggct gggccgtgag
      421 cgtcacggtc gtgcccgccg cgaacgaggt cgcgcaggtc gagccgcagt tgatggccga
      481 cggcgacgag atcaccgtgc cggcgccgtt gcccttgccg acgaccgtga gcgcgtattg
      541 cgtcgtggtg ggcgggggcg gcggcggcgt cgtttcggta ggcacggtgg ccccgcccga
      601 gatcgacgat ccgctggagg cggcgatccc gaacttcatc gccggggcgc ccgactggtg
      661 cgcgcccacg tcgggcgtgg cggcgccgtc gttgaagttg gggatgcgcg cgccgcggcc
      721 gtagccgggg ctgctcgcgt cgagctggta gttgccgccc gcctcgctct gccagccgtg
      781 gcccgcggca tacaccggcg tgcccacgat gccgctcgtg acggtggcat caccggccgt
      841 gccgttgtac atgtcggaga cgaaggagct gctggagccc gtctggtacg tgaagccctt
      901 gccttccttc cactggtggt agatgttgtt gcgcgagaag gtgttctcga tcagctgcgc
      961 cgagcccgtg ccgcccaggc cgaagccgcc gcccaggccg tattgcaggc ccgactgccg
     1021 cgcctgcagc atcgtgttgt ggaagatgta gcgccggccg tggcccagcg tgttgtcgcc
     1081 gccggacttg aagaagggct ggcggtcgtc ggcgtcgagc gccgccttct cgtacatctg
     1141 cgcgcggttc cacacgttgc ggaagatgta gacggggccg atggaagtcg ccgtggtggc
     1201 cacgccgatc gcggtgcggt cgatgtagtt gccccagatg cgcacgttct cgttggcgcc
     1261 ctcggattcg atcccgtcgt cccaggcgtg cgagagcttg ttgccgtaga tgtcggtgtc
     1321 ggagttgggg aagcccgcct tggtgaagtt gtcctcgccg ccgaagatgt cgttgaagta
     1381 gttgccgttg gtgctgtagg cctcgttgtg gcggaagacg tggttgccgc cgcagtggct
     1441 gaaggagatc gcctggggac cggccgggtg cccgtcggac cagctgttgg cgctgtagcg
     1501 cgggtcgtgc atcttgttgc gctggatggt cacgcgcatg agggtgggct gcgtgcacac
     1561 ggcgcggatg gcggagtcca tgtccacgcc ccagttgccg gagcgctgcc gcccccagcc
     1621 gctgatgtcg ttgtcctcga tcaccacgtc ggtcacgttg ggcgagatgc ggatcgcgtc
     1681 ctgcttggcg cccttgaggt tgaggccgcg cacgatcacg taggaggcgt tgatcgtcac
     1741 gttgtactgg gcgctgttct ggccatcgag ggtggcgccg ttgccttcgt agaccacgta
     1801 gcccgtggag gcgctgccgc cctcggcgac gttgagcgtc gcggagccgg cgttcacggc
     1861 gatggtcttg gccaccggaa gctggttcga ccaggtcttg aagaccagcc cgtgcgtcgc
     1921 cgcctggccc ggcaggttga actcgacctg gtagtaggtg ttgggagcca ggctcacgag
     1981 gctgccgcgg cactgcgagt cgcgggcgtc gtaccagagc ggcagtccct gcgaccagga
     2041 cgcctcaccc gccttgcgga acttgacctc gcagccattg ctgccgcccg ggttctgcca
     2101 gtacaggccc gcggcctcgt acgtcggaac cgcgatggtg ccggtggacg gcagggtcgc
     2161 cgcgggggcc tgcgccttgg cggcgccgca gtaagcggcg aaggcgacga tcaggaacga
     2221 ggctccggcc agcgtgcgcg cgcggttatc ggagaaggtc tggttgggga catgtcgttg
     2281 catgattctc tccaagggga aactcgagac gagccacccc ataagcgaga gccatgccag
     2341 cgtctttttt tctttatcaa tcaactactt ggtgcaactt attgatgggt gcattaacaa
     2401 cgtctgtagc gcgcccaaca caccaatgta ggtgaccccc gatttcgttg taggaaacca
     2461 ctgtacggga gtcagagccc tcctacaacc tgtagcgatt cggcgacaaa gggtcgattt
     2521 tccgtgacgg tt
//
LOCUS       DASUEV010000063         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_18762, whole genome shotgun sequence.
ACCESSION   DASUEV010000063 DASUEV010000000
VERSION     DASUEV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18762"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..476
                     /locus_tag="VFV55_01935"
     CDS             165..476
                     /locus_tag="VFV55_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX4943080.1"
                     /translation="MSGTLLQLCPAGDVPEGGALKVERDGLILAVFNLGGRYFVTDDA
                     CTHGPGSLSEGEIDGEIVECNFHNGAFHIPTGRVEAPPCMVPLKTYATRVVDGQVCIE
                     A"
     gene            complement(477..872)
                     /locus_tag="VFV55_01940"
     CDS             complement(477..872)
                     /locus_tag="VFV55_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4943081.1"
                     /translation="MDRSPLKVFIADDSKPVADMLTELLSAPGRIEVVGIGATEDAAI
                     ESIRQMKPDVVVLDLQLKTGSGTNVIRAVRASQDLAGTKLMVASNHTAPQLKAGCLEL
                     GADGYYDKVKELGALAACLGELADAKQKR"
     gene            1063..1464
                     /locus_tag="VFV55_01945"
     CDS             1063..1464
                     /locus_tag="VFV55_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4943082.1"
                     /translation="MTILKVSHDSPMREAARAALRNAGIAVLELDDWDQADALGRGET
                     ALVICDGEAIRQLKESPSSPLPRDALRKLSHDLRTPLSAMAGWLHLMESGSLDAAGLK
                     RAIERIKGNIDEQVRTIDRCLGTNQGEGQRK"
     gene            1461..>2694
                     /locus_tag="VFV55_01950"
     CDS             1461..>2694
                     /locus_tag="VFV55_01950"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX4943083.1"
                     /translation="MKTKTVNETGSTVARKRVADKSRQPAQPKGNGAAAPDSLSQQEA
                     KAILQALTALKQGDSEVRLPVEWTGLQGKVSETFNEVVELNARMAEELARLRQKVGKE
                     GKLKQRAEMSEVRGFWRDQIHCVNALIDDLVHPTSETARVIGAVAQGDLSQTMALEVD
                     DRPLEGEFLRTARTINKMVDQLASFASEVTRVAREVGTEGKLGGQAKVKGVAGTWKDL
                     TDSVNSMAGNLTGQVRNIAEVTTAVATGDLSKKITVDVKGEVLELKNTINTMVDQLRS
                     FASEVTRVAREVGTEGRLGGQADVQGVAGTWKDLTDSVNSMAGNLTGQVRNIADVTTA
                     VARGDLSRKITVDVKGEILELKNTINTMVDQLSSFASEVTRVAREVGTEGRLGGQADV
                     QGVAGTWKDLTDSVNSMAG"
BASE COUNT          487 a          845 c          927 g          435 t
ORIGIN      
        1 gagttcgttg tgctcggctt tcattcgctt tccttggctg cccacggcgt tcttctataa
       61 ggaacggcgt tcctttctgt ggtcaccact ttagcaaacc ggctagaatg cggcaacgct
      121 gcgcgccccg gaaaggcgcc gtttccaccc actcgccaga caccatgtcc ggaacgctgc
      181 tccagctttg ccccgcgggg gatgtccccg agggtggcgc gctcaaggtc gagcgcgatg
      241 gcctgatcct ggcggtcttc aacctcggcg ggcgttactt cgtcaccgac gacgcctgca
      301 cccacggacc gggctcgctc tccgaaggcg agatcgacgg ggagatcgtg gaatgcaact
      361 tccacaacgg cgccttccac attcccacgg ggcgcgtcga ggcgcccccc tgcatggttc
      421 cgctgaagac ctacgccacc cgcgtggtcg acggccaggt ctgcatcgag gcctagctac
      481 cgcttctgtt tcgcgtcggc gagctcgccc aggcaggcgg ccagcgcgcc cagctccttg
      541 accttgtcgt agtagccgtc ggcgcccagc tccaggcagc cggccttgag ctggggggcc
      601 gtgtggttcg aagccaccat gagcttggtg ccggccaggt cctggctggc cctcacggcc
      661 cggatcacgt tggtccccga gccggtcttc agctggaggt cgaggacgac gacgtccggt
      721 ttcatctggc ggatcgattc gatcgcggca tcctccgtcg ccccgatgcc gaccacctcg
      781 atgcggccgg gcgccgacaa cagctcggta agcatgtcgg ccaccggctt ggaatcgtcg
      841 gcaatgaata ccttcagggg actgcggtcc attcttttct ttcggccgaa tcggcgcccg
      901 gcgtaatttt aggtgagtcc agagggctca tgtcggaaac agttacctgt aggccgcctc
      961 ctacattttg tccgagaacc ccggcctata ctcgaaatca ctttgctttc atggtgaatt
     1021 tcgctccgaa gaagggcgga aatcccgcgc acatccatga cgatgaccat ccttaaggtg
     1081 tcccatgatt ccccgatgcg ggaggcggcg cgtgctgcct tgcgcaacgc cgggatagcg
     1141 gtgctcgagc tggacgactg ggaccaggcg gacgccctgg gccgcggcga gacggccctg
     1201 gtcatctgcg acggcgaggc gatccgccag ctgaaggagt cgccttcgag cccgttgccg
     1261 cgcgatgccc tgcggaagct ttcgcacgac ctgcgaacgc cgctttcggc gatggccggg
     1321 tggctgcatc tgatggaatc gggaagcctc gatgccgcgg gcttgaagcg ggccatcgag
     1381 aggatcaagg gcaacatcga tgagcaggtg cggaccatcg atcgctgcct cgggaccaac
     1441 cagggggaag gtcaacgcaa atgaagacca agaccgtgaa cgagaccggg tccacggtgg
     1501 cgcgcaagcg cgtcgcggac aagtcccgcc agcccgcgca gccgaagggg aacggcgccg
     1561 ccgcgccgga ttcgctttca cagcaggaag cgaaggccat cctgcaggcg ctcaccgcgc
     1621 tcaagcaggg cgacagcgag gtccggctgc cggtcgagtg gaccggcttg cagggcaaag
     1681 tatccgagac cttcaacgag gtcgtggagc tcaacgcgcg catggccgag gagctcgcgc
     1741 ggctgcgcca gaaagtgggc aaggagggca agctcaagca gcgcgcggag atgagcgagg
     1801 tgcgcggctt ctggcgcgac cagatccatt gcgtgaacgc cctcatcgac gacctcgtcc
     1861 acccgacttc ggaaaccgcg cgcgtgatcg gcgcggtggc gcagggcgac ctgtcccaga
     1921 ccatggcgct cgaggtggac gaccgtccgc tcgagggcga attcctgcgg accgcgcgga
     1981 ccatcaacaa gatggtggac cagctcgcgt cgttcgcctc cgaggtgacg cgggtggcgc
     2041 gcgaggtggg caccgaaggc aagctcggcg gccaggcgaa ggtgaagggc gtggccggca
     2101 cgtggaagga cctcaccgac tcggtgaact ccatggccgg aaacctcacg ggccaggtgc
     2161 gcaacatcgc ggaggtgacc acggcggtgg cgaccggtga cctctccaag aagatcaccg
     2221 tggacgtgaa gggcgaggtg ctggagctga agaacaccat caacacgatg gtggaccagc
     2281 tgcgttcgtt cgcctcggaa gtgacgcgcg tggcgcgcga ggtgggtacc gaggggcggc
     2341 tcggcggcca ggccgacgtg cagggtgtcg cgggcacgtg gaaggacctg accgactcgg
     2401 tgaactccat ggccggcaac ttgacgggcc aggtgcgcaa catcgccgac gtgacgacgg
     2461 ccgtggcgcg gggggacctg tcgcgcaaga tcacggtgga cgtgaagggc gagatcctgg
     2521 agctcaagaa cacgatcaac acgatggtgg accagctctc gtcgttcgct tcggaagtga
     2581 ctcgagtcgc gcgagaagtg ggtaccgaag gacggctggg cggccaggcc gacgtgcagg
     2641 gtgtggcggg cacgtggaag gacttgaccg actcggtgaa ctccatggcc ggca
//
LOCUS       DASUEV010000064        11498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_592069, whole genome shotgun sequence.
ACCESSION   DASUEV010000064 DASUEV010000000
VERSION     DASUEV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592069"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFV55_01955"
     CDS             <1..475
                     /locus_tag="VFV55_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943084.1"
                     /translation="MRGLPRLDAVQRPVFGALAASMGLFAAGGLIGFAIGGPNVKIPA
                     HYHGCIVGVTLAFMGLAYHLLPKLGYAAPRSRLAAWQPWLYGSGQLMHIVGLVWSGGY
                     GVERKVAGAAQVLRTPQEVAGMALMGLGGLVAIVGGLAFVIVAIGALRRRAAAVR"
     gene            472..1287
                     /locus_tag="VFV55_01960"
     CDS             472..1287
                     /locus_tag="VFV55_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943085.1"
                     /translation="MSEPGPARDDRTLGAAAAACAALVFVVVASSAWLRLVAVPCPPA
                     GCDGLTLADAVRLSHRVAAMGVSVLALVIAAVAWKSPARRGRRISAVALLLLVAVLAL
                     IGRRSAGVASPAVMLGNLFGGLALLALATGLAIAVRTPRGRLSIPFVAAIVLFAAAII
                     TGGLLATAIQADSSSLGIAHRALSWVALAVWSFLGLGAAQQPGVRLVSRLTAILIAAQ
                     VVLALAFPGWPVVRWLHNFLTSAALCSAIAAMLASRANAPEDAGLSGRAPAGS"
     gene            1321..2055
                     /locus_tag="VFV55_01965"
     CDS             1321..2055
                     /locus_tag="VFV55_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4943086.1"
                     /translation="MPIAQAVMQPGEPRTRGPDIPVEQFLVNLPLFSDLTPGEIQRIA
                     AGTRRLYATKGERLFSRGDRCEGFHVVLYGQVKLFVTSPQGSEKVIEIMGPGISFGEA
                     ILFMDMPYVVSAEALKDSLLLHVSRSVVFDEIERDPRFARRLLAGMSRKLHQLIRDVE
                     AYSLRSGLERVIGYLLRDDALSDGSARSTSMTLPANKMIVASRLNLTPEYFSRILHEL
                     SDSGLIVMEGRSVSVPDIERLRTYTG"
     gene            2067..3227
                     /locus_tag="VFV55_01970"
     CDS             2067..3227
                     /locus_tag="VFV55_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4943087.1"
                     /translation="MATRTQPRLKRAPRVAEDAALPEVGLKAAALDVSDDGIVISDAS
                     SPDAPVVYVSPSFERLVGYSASEILGTNCRMVQGPGTDPDTVREIAIALQQGKVFQGE
                     ILNYRKDGTPFWNFLRIAPIRDASGRVTHHVGTQSDVTEQHLARERQRELQDALAHAS
                     RVRTVAALGASLAHEVNQPLAAIVTNAEAALRFLDAGNVAEVRDALEAIAADAHRAGE
                     IILRAHRLVRKQPATADPVDPVRIASEVLEFTRTRMRHAGVRARCEARKAIPRVMADP
                     VQLYQAILNLVVNAIEAMENEPPGFERSLVVAAEPEKGGVRVRVTDTGHGADDEDLAR
                     MAQTFHTTKARGTGLGLLVTRSLVEAHGGRLDVARNAGRGLTFTIHLPGETP"
     gene            3224..3877
                     /locus_tag="VFV55_01975"
     CDS             3224..3877
                     /locus_tag="VFV55_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4943088.1"
                     /translation="MIAAAGVNLFIVDDDPSVRASLTRLLRAEGADPQAFASAEAFLE
                     HLPRITGPSCALLDIGLPGKSGLELHARLQATRPEFAVVFLTGHGDVPGAVASMKRGA
                     VDYLLKPVETATLLAALTRAIERARAASSEKTTKREIALHYGTLTPRQREVLCHVIAG
                     KRNRVIAEEIGTTEKTVKVHRGRIMEKMQVRSVADLVRAADKLGIKPAPSPPGTKAP"
     gene            3874..5007
                     /locus_tag="VFV55_01980"
     CDS             3874..5007
                     /locus_tag="VFV55_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4943089.1"
                     /translation="MSQPVHQDALQALNQEGTLRARVREIHGAVRTFMPFVARIAVAI
                     YDPKTTLLKTFVHSSEGDNPLPHYQAPLGEVPSLKKLLEEGHPRVIQHMLTFEDAESE
                     HAKRLGRAGYAASYTLPIFWNGSFLGFVFFNSRKPDAFTETALTQLDVFGHLVSLLVI
                     QEFAAIRTLSAALESVRHIMNARDPETGSHLDRMSRYARLIAHALAERHGLDDDYVEC
                     IQAYAPLHDIGKIGIPDSILLKPASLTDDETAVMRTHSNRGQAMIDDLVTNFGLGTLA
                     STEVVRNIALLHHERMDGKGYPYGLAGDAIPLEARIVAVADVFDALTSRRPYKEAYAN
                     DVAFMMIAQLANTHLDPECVQALVANRAEVERIQAQFREDPIG"
     gene            complement(5008..6303)
                     /locus_tag="VFV55_01985"
     CDS             complement(5008..6303)
                     /locus_tag="VFV55_01985"
                     /inference="COORDINATES: protein motif:HMM:NF028221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX4943090.1"
                     /translation="MNRPLARPLDRAEAFFWFLDRCSSMNFAVMAEGFGPLQAAQLEA
                     ALDRAQAVHPALSVAIEADAAGRLAFVPHPGRRVALSREDAGADWRSAFAALLVQPFA
                     AGQAPLLRAHWFERTDGRWAFAVVFHHAIGDGRSGFRLVTDLIDDARGVRTTPAHHVP
                     RPSLMALFPEDLAGSAGLARAQEWKAALRGGTLRPGPFPGFTRDGGPVRPGIVAVHFD
                     ADVVAGLSRRAREIGASVHGLIGAAELIAARNCFGAGETPALMLTSPVDLRGNLAAPV
                     DDATPGFFVTLLSSIATVGGPEDLPRLAIFLTDDLRRQVAEGRGHLFYHLVPPAETIP
                     ATAEGIAGFAAYMQRMPTAFVLSNVGRVAALPEANGVRVDEVSFALCPMAHQPLFVAA
                     STWGGRLTLNVVHDAARFAPRAAQEIAAGIAAQLRQAAG"
     gene            6377..6685
                     /locus_tag="VFV55_01990"
     CDS             6377..6685
                     /locus_tag="VFV55_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX4943091.1"
                     /translation="MPLLDADGYLVDPLQWTEEIALALARREGIELGPDHWDAIRFMR
                     RYWEEHQIAADARFVIRHLMERLGPESRNRLFELFPYGYPGQACKIAGMKRPRAWSTG
                     "
     gene            complement(7297..8172)
                     /locus_tag="VFV55_01995"
     CDS             complement(7297..8172)
                     /locus_tag="VFV55_01995"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4943092.1"
                     /translation="MRMLVAMLALAAAVPVHAQLDLGRLLDLGKKAVEVGQQVQEATK
                     EYTVEEEVALGEGIASGFLGAARLHPDERLQRYVNRVGRWLASQTERPDLPWAFGVID
                     TDTINAFAMPGGTVVVSHGLVKRLTSESELAGVLAHEIAHVLKKHQLSAIQSDAGWSA
                     AATGAKTYAADRIGRSGDVMGLKSRLANAGVDMVKDGLFLRPLDRSMEYEADRLGVVI
                     ATRGGYDPYGFVAVLSMLAQVKPEESGASITFSTHPSPADRLAELEKVVPAALEGYAR
                     QPQVEGRFRQVVGAK"
     gene            complement(8169..8693)
                     /locus_tag="VFV55_02000"
     CDS             complement(8169..8693)
                     /locus_tag="VFV55_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943093.1"
                     /translation="MPHRLILLLLLACLPLVGTAYAQEQASTNRATDLKERPAPDSRT
                     LAPLAADSPVKVLSRQGGWTQVESGAQRGWVRAFHLRFQSTVETAPSGNALGGLTSMF
                     GFGSRKAPETSRVATLGIRGLTPEDFKNASPDLAALKKLQSWRVDKASAERFAKEAKL
                     VPVAVAYQREGGGS"
     gene            complement(8755..9969)
                     /locus_tag="VFV55_02005"
     CDS             complement(8755..9969)
                     /locus_tag="VFV55_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX4943094.1"
                     /translation="MSQQIPEARQTPGASLKSQPPREAARMLAAEEPARAVELLAELN
                     PGRAQDVLAELPEGALAAIVRAAAPENAKQWMRNARYSRDAIGRLMEPAYAVFSPGMS
                     VGEAIEQLRAVVRTAFVTYGYVTDGEGRLLGIVTMRDLLFNEHGAKLGDVMLRDAFFF
                     RPDTPLSDAMKLTLNRHYPVYPVCDDSGRLLGLVRGAAIFEEQAFEITAQPGSMVGVE
                     KEERVNTTLGRAFRFRHPWLQLNLLTAFAAGGVVAMFQGTVDRLVILATFLPVLAGQS
                     GNTGCQALAVTVRGMTLGDIRAGAGRALVAKEFFLGLSNGGLTGVLCGAAMFFLARAQ
                     ESPHALALGLAVFLAMMGSCAVSGVSGALVPLALKRLGADPATASSIIVTTATDVASM
                     GMLLGFATVLVR"
     gene            complement(10018..10863)
                     /gene="metF"
                     /locus_tag="VFV55_02010"
     CDS             complement(10018..10863)
                     /gene="metF"
                     /locus_tag="VFV55_02010"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX4943095.1"
                     /translation="MQTQRNRPKLFSFEFFPPRTPEGDEKLRKARLQLAQLKPAFMSC
                     TYGAGGSTQEGTLRTILEIHREGQVAAPHISCIGSKRETIAALLDQYRAAGIRRLVCL
                     RGDLPSGMAVAGEFRYAAELVGFVRNHTGDWFHIEVAAYPEFHPQARRPADDLAHFKA
                     KIEAGANSAITQYFYNADAYVRFVESVEALGVDVPIVPGVMPINNFTQLARFSDACGA
                     EIPRWIRLRLESFGDDTASIKAFGLDVVTELCDRLLTLGAPGLHFYTLNQAGPATTIW
                     QRLGL"
     gene            complement(10906..>11498)
                     /locus_tag="VFV55_02015"
     CDS             complement(10906..>11498)
                     /locus_tag="VFV55_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX4943096.1"
                     /translation="LSAQVWVTEIDPICALQASMEGYRVVTMDYAADKADIFVTATGN
                     FHVITHEHMSRMKNQAIVCNIGHFDNEIDVASLKKYKWENIKPQVDHIIFPDGKRIIL
                     LAEGRLVNLGCGTGHPSYVMSSSFANQTLAQIELYGHTDKYPIGVYVLPKHLDEKVAR
                     LQLKKLTAQLTELTDEQARYIGVAREGPYKAELYRY"
BASE COUNT         1754 a         4071 c         3899 g         1774 t
ORIGIN      
        1 gatgcgcgga ctgcccaggc tcgacgccgt gcagcgcccg gtcttcggcg cgctggctgc
       61 ctcgatgggc ctcttcgcgg caggcggcct gatcggcttc gcgatcggcg gccccaatgt
      121 gaagatcccc gcgcactacc atggctgcat cgtcggcgtg accctcgcct tcatgggact
      181 tgcgtatcac ctgctcccga agctcggcta cgcggcgccg cgctcgcggc tggccgcatg
      241 gcaaccctgg ctctacggat ccgggcaact catgcacatc gtgggcctcg tgtggtccgg
      301 cgggtacggc gtcgaacgca aggtggccgg cgccgcgcag gtcttgcgca cgccgcagga
      361 ggtcgccggg atggcgctga tgggcctcgg cggcctcgtg gccatcgtcg gcgggctcgc
      421 attcgtgatc gtggcgatcg gcgcgcttcg ccggcgcgct gccgcggtcc ggtgagcgag
      481 cccggccccg cgcgcgacga ccgcacgctt ggcgcggcag cggcggcttg cgcggcgctc
      541 gtcttcgtcg tcgtcgcgtc cagcgcctgg ctgcggctcg tggccgtgcc ctgccccccg
      601 gccggctgcg acggcctgac gctcgccgat gcggtgcggc tctcccaccg cgtggcggcc
      661 atgggcgtga gtgtcctcgc cctggtcatc gccgccgtcg cgtggaagtc tcccgcacgt
      721 cgggggcgcc gcatctcggc cgtcgccctc ctcctgctgg tggccgttct ggccctcatc
      781 gggcgacgct ccgcgggcgt tgcttcaccg gcggtgatgc tcggcaacct gttcggcggt
      841 cttgcgttgc tcgcgctcgc cacgggcctc gccatcgcag tgcgcacccc gcgcggccgc
      901 ctctccatcc cgttcgtcgc cgccatcgtg cttttcgctg ccgcgatcat caccggcggc
      961 ctgctcgcca ccgcgatcca ggccgactcc tcctcgctgg gcatcgcgca ccgcgcgctt
     1021 tcctgggtgg ccctggccgt gtggagtttt ctcgggttgg gcgctgcgca gcagcccggc
     1081 gtgcgcctgg tcagccggct caccgcaatc cttatcgcgg cgcaggtcgt gctcgccctc
     1141 gcgttccccg gctggccggt cgtgcgctgg ctgcacaact tcctcacctc ggcggcgctt
     1201 tgctcggcca tcgccgcgat gctcgcatcg cgagccaacg cccccgagga tgccgggctg
     1261 tccggacgcg cacccgccgg gtcatgagcg caagccgggt tatcatgtgc acacaacccc
     1321 atgcccatcg cccaagccgt catgcaaccc ggagaaccgc gtacgcgcgg gcccgacatt
     1381 ccggtcgagc agttcctcgt caacctgccg ttgttcagcg acctcacgcc cggggaaatc
     1441 cagcgcatcg ccgccggaac gcgccgcctc tatgcaacga agggtgagag gctgttcagc
     1501 cgcggcgacc gctgcgaggg gttccacgtc gtcctctacg ggcaggtgaa gctcttcgtc
     1561 acctcgccgc aaggctcgga gaaggtgatc gagatcatgg gtccgggcat ctcgttcggc
     1621 gaggcgatcc tcttcatgga catgccctac gtggtctccg ccgaggcgct caaggattcc
     1681 ctgctgctgc acgtctcgcg aagcgtggtc ttcgacgaga tcgagcggga cccgcgtttc
     1741 gcccgccggc tgctggccgg catgtcgagg aagctgcacc agctcatccg ggacgtggag
     1801 gcgtattcgc ttcgctccgg cctcgagcgc gtgatcggct acctgctgcg cgacgatgcc
     1861 ctgtccgacg gctcggcgcg ctcgacgagc atgacgctgc ccgccaacaa gatgatcgtg
     1921 gcttcgcggc tcaacctcac gccggaatac ttctcgcgca tcctgcacga actgtccgat
     1981 tccgggctca tcgtgatgga aggccgctcg gtgagcgtgc cggacatcga gcgcctgcgc
     2041 acctacaccg gctagccgcg acgacgatgg ccacccgcac ccagccccgg ctgaagcgcg
     2101 cgccccgcgt cgccgaggac gcggccctgc ccgaggtcgg gctcaaggcc gccgcgctgg
     2161 acgtgtcgga cgacggcatc gtcatatcgg acgcctcgag cccggacgcg ccggtcgtct
     2221 acgtgagccc gtcgttcgag cggctcgtgg gctactccgc cagcgagatc ctcggcacca
     2281 actgccgcat ggtgcagggg ccgggcaccg acccggacac cgtccgggag atcgcgatcg
     2341 cgctgcagca gggcaaggtc ttccagggcg agatcctcaa ctaccgcaag gacggcacgc
     2401 ccttctggaa cttcctgcgc atcgccccca tccgcgacgc cagcggcagg gtcacccacc
     2461 acgtgggcac gcagtccgac gtgaccgaac agcacctcgc ccgcgagcgg cagcgcgagc
     2521 tgcaggacgc gctcgcccac gcgtcgcgcg tgcgcacggt ggccgcgctc ggcgcgtccc
     2581 tcgcccacga ggtgaaccag ccgcttgcgg ccatcgtcac caacgccgag gcggcgctgc
     2641 gctttctcga tgccggcaac gtggcggagg tccgcgacgc cctcgaggcc atcgcggcgg
     2701 acgcccatcg cgccggcgag atcatccttc gcgcgcaccg cctcgtgcgc aagcagccgg
     2761 ccacggccga tcccgtggac ccggtgcgaa tcgcttccga ggtcctcgaa ttcacccgca
     2821 cgcgcatgcg ccatgccggc gtcagggcga ggtgcgaggc gcgcaaggcg atcccgcgcg
     2881 tgatggccga tcccgtccag ctctaccagg cgatcctcaa cctcgtcgtg aacgcgatcg
     2941 aggccatgga aaacgagcct cccgggttcg agcgcagcct cgttgtcgcg gccgagcccg
     3001 aaaagggcgg tgtgcgcgtc cgcgtgaccg ataccggcca cggcgccgac gacgaggacc
     3061 tggcccgcat ggcgcagacc ttccacacca cgaaggcgcg cggcaccggc ctggggctgc
     3121 tcgtcacgcg gtcgctcgtg gaagcgcatg gcggccggct cgacgtggcg cgcaacgccg
     3181 gccgcgggct gacgttcacc atccacctgc cgggagaaac cccatgatcg ccgccgcggg
     3241 cgtgaacctc ttcatcgtcg atgacgaccc gtccgtgcgc gcctcgctca cgcggctgct
     3301 tcgcgccgag ggcgccgacc cgcaggcctt cgcctccgcc gaggcgttcc tggaacacct
     3361 gccgcgcatc acggggcctt cctgcgccct gctcgacatc gggcttcccg gcaagagcgg
     3421 cctcgagctg cacgcgcggc tgcaggcgac gcggcccgag ttcgcggtcg tgttcctgac
     3481 cggccacggc gacgttccgg gggcggtcgc gtccatgaag cgcggggcag tcgactacct
     3541 gctgaagccc gtcgagacgg caacgcttct cgcggccctg acgcgcgcca tcgaacgcgc
     3601 ccgcgcggcc tccagcgaaa agacaaccaa gcgggaaatt gcgctgcact acggcacgct
     3661 cacgcctcgc cagcgcgaag tgctctgcca cgtgatcgcc ggcaagcgca acagggtgat
     3721 cgcggaggag ataggcacca ccgagaagac ggtcaaggtg caccgcggcc ggatcatgga
     3781 gaagatgcag gtgcgctccg tggccgacct ggtgcgcgcc gccgacaagc tggggatcaa
     3841 gcccgcaccg tccccgccgg gaacgaaggc gccatgagcc agcccgtcca ccaggacgct
     3901 ctccaggcgc tcaaccagga agggacgctg cgcgccaggg tccgcgagat ccatggcgcg
     3961 gtgcgcacct tcatgccgtt cgtggcccgg atcgcggtcg ccatctacga ccccaagacg
     4021 acgctgctca agacgttcgt ccactcgagc gaaggcgaca acccgctgcc ccactaccag
     4081 gcgccgctcg gcgaggttcc gtcgctcaag aagctgctcg aggaagggca tccgcgcgtc
     4141 atccagcaca tgctcacctt cgaggacgcc gagagcgaac acgcgaagcg cctggggcgc
     4201 gccggctatg ccgcgagcta cacgctgccc atattctgga acggctcctt cctcgggttc
     4261 gtgttcttca attcacgcaa gccggacgcg ttcaccgaga ccgcgctcac gcagctcgac
     4321 gtcttcggcc acctggtgtc gctgctggtg atccaggagt tcgccgccat ccgcaccctg
     4381 tcggccgcgc tcgagagcgt gcggcacatc atgaatgcgc gcgatcccga gaccggcagc
     4441 catctcgacc ggatgtcccg ctatgcccgc ctgatcgccc acgcgctcgc ggagcgccac
     4501 ggcctcgacg acgactacgt cgagtgcatc caggcctacg cgccgctgca cgacatcggc
     4561 aagatcggca ttccggactc catcctgctc aagccggcca gcctcacgga cgacgagacg
     4621 gcggtgatgc gcacgcacag caatcgcggc caggcgatga tcgacgacct ggtgaccaat
     4681 ttcgggctgg gaacgctggc aagcaccgag gtcgtgcgca acatcgccct gctccaccac
     4741 gagcgcatgg acggcaaggg ctacccgtac gggctcgccg gcgacgcgat cccgctggag
     4801 gcgcgcatcg tggcggtggc ggacgtattc gacgccctga ccagccggcg cccgtacaag
     4861 gaagcctatg ccaacgacgt ggcgttcatg atgatcgcgc agctggcgaa cacgcacctc
     4921 gaccccgaat gcgtgcaggc gctcgtcgcc aaccgcgccg aggtcgagcg catccaggcg
     4981 cagttccgcg aggacccgat cggctgatca accggccgcc tgccgcagtt gcgccgcgat
     5041 gcctgcggcg atttcctgcg cggcccgtgg cgcgaaccgg gcagcgtcgt gcacgacgtt
     5101 gagcgtgagc cttccacccc aggtgctcgc ggccacgaag agcggctggt gcgccatcgg
     5161 gcacagggcg aacgagacct cgtccacgcg cacgccgttg gcctccggga gagccgctac
     5221 gcggcccacg ttgctcagca cgaacgccgt cggcatgcgc tgcatgtagg ccgcgaagcc
     5281 cgcgatgccc tccgccgtcg cgggtatcgt ctccgccggc ggcacgaggt ggtagaagag
     5341 gtggccgcgc ccctcggcaa cctggcggcg caggtcgtcg gtgagaaata tcgcgagcct
     5401 cgggaggtct tccgggccac cgaccgttgc gatggacgag agcagcgtca cgaagaaacc
     5461 cggcgtcgca tcgtcgaccg gcgcggcgag gttcccgcgc aggtccaccg ggctcgtgag
     5521 catgagggcc ggcgtttcgc ctgcgccgaa gcagttgcgc gcggcgatga gttccgccgc
     5581 gccgatgagc ccgtggacgc ttgcacctat ctcccgcgcg cggcgggaga ggcctgcgac
     5641 gacatccgca tcgaaatgca cggccacgat gccgggtcgc accggtccgc catcgcgcgt
     5701 gaagcccgga aacggccccg ggcgcagggt cccgccccgc aatgcggcct tccattcctg
     5761 cgccctggca aggcccgcgg acccggcgag atcttccggg aagagcgcca tcagggaagg
     5821 acgcggtacg tggtgcgcgg gggtggtgcg cacgccgcgt gcatcgtcga tcaggtcggt
     5881 caccagacga aatcccgagc ggccgtcgcc gatggcgtgg tggaagacga cggcgaatgc
     5941 ccagcgacca tcggttcgct cgaaccagtg cgcgcgaagc agcggcgcct ggccggccgc
     6001 gaaaggctgc accagcagcg cggcgaatgc ggagcgccag tcggcgccgg cgtcctcacg
     6061 agacaaggcc acgcgcctgc cgggatgcgg gacgaaagcc aggcgcccgg ccgcgtctgc
     6121 ctctatcgca accgaaaggg ccggatggac ggcctgcgcg cgatcgagcg cggcttccag
     6181 ctgcgccgcc tgcagcggac cgaaaccttc cgccatcacg gcgaagttca tcgacgagca
     6241 ccggtcgagg aaccagaaga aggcctcggc acggtccagc gggcgggcga gcggtcggtt
     6301 cacgggctac ccgcgatggg ggattgacgg cgcattccgt ttcgttaaca tacggctcat
     6361 ggaaggtgta tccaacatgc cgcttctaga cgccgatggc tacctcgtcg acccgctcca
     6421 gtggaccgag gaaatcgcgc tcgccctcgc gcggcgcgaa ggaatcgaac tgggccccga
     6481 ccactgggac gcgatccgct tcatgcgccg ctactgggaa gagcaccaga tcgcggccga
     6541 cgcgcgcttc gtgatccggc acctcatgga acgcctcggc ccggaatcgc gcaaccggct
     6601 cttcgaactc tttccctacg gctaccccgg ccaggcctgc aagatcgccg gcatgaagcg
     6661 tccccgcgcc tggagcaccg gctgaccagg gtgaggcgat ggcggtggaa gtcgatcgca
     6721 atcgtgggca tgtgcggtga ggcggtggcg gtggaagtcg atcgcaatcc tgggcacgca
     6781 agcgtgaccg tcggatgggt gcccggaaag tcgagtccag ccaaacacgt ctggcctcga
     6841 ctttccaccc atctagagac gagggcgctg cgctgcctcg tccgcgacgg tcgcgtgcca
     6901 aggattgcga tcgacttcca ccgccaccgc ctcccacgct ccgtggatac cgcagcgagt
     6961 gaggcaacat tggaccgcgt catcgttgct gactgcgcca cgctgcctat tcatgttggc
     7021 cggggatggg gcaagttaat cggcgtcctt gggcacgcga ccgtcgggat gggagagcgc
     7081 agcggcccca tctctcaatg ggtaaaaagt cggggacaga cgtgtttggc tggccccgac
     7141 tttttgggac ccattcgacg gtcacgcttg cgtgcccagg acgccgatag acttgcccca
     7201 tccccgggca tccttccatt cgccgtccgt agccggcacc gggaatgcca aaaagggcgc
     7261 cctgcggcgc ccttttcctc gtgcacggcg ggagcgctat ttcgcgccga ccacctggcg
     7321 gaagcggcct tcgacttgcg gctgcctcgc gtacccctcg agcgccgccg ggaccacctt
     7381 ctcgagctcg gccaggcggt cggccgggga gggatgggtc gagaacgtga tcgaggcgcc
     7441 ggattcctcc ggcttcacct gcgcgagcat ggagagcacc gcgacgaagc cgtaggggtc
     7501 gtagccgccg cgcgtggcga tgaccacgcc gagccggtcc gcctcgtact ccatgctgcg
     7561 gtcgagcggg cgcaggaaca gcccgtcctt caccatgtcc acccccgcgt tggcaagccg
     7621 ggacttgagt cccatcacgt cgccgctgcg cccgatgcgg tccgccgcat aggtcttggc
     7681 ccccgtggcg gcggcgctcc acccggcgtc ggactggatg gcggacagct ggtgcttctt
     7741 cagcacgtgc gcgatctcgt gggcgagcac accggccagc tccgattcgc tcgtgaggcg
     7801 tttcaccagc ccatgcgaga cgacgaccgt tccgccgggc atcgcgaagg cgttgatggt
     7861 atcggtgtcg atcacgccaa acgcccatgg caggtcgggg cgctcggtct gcgaggcgag
     7921 ccagcgcccg acgcggttca cgtagcgctg caggcgctcg tcgggatgca gccgggcggc
     7981 gccgaggaag cccgaggcga ttccctcgcc gagggccact tcctcctcca ccgtgtactc
     8041 cttcgtcgcc tcctggacct gctgccccac ctcgacggcc ttcttgccga ggtcgaggag
     8101 gcggccgagg tcgagctgcg cgtgcacggg aaccgccgcg gcgagcgcca gcattgccac
     8161 cagcattctc atgagccgcc tccctcgcgc tggtaggcga cggcgacggg cacgagcttc
     8221 gcctccttcg cgaagcgctc ggcggaagcc ttgtcgacgc gccaggactg cagcttcttc
     8281 agcgccgcca ggtcgggcga ggcattcttg aagtcctccg gcgtgagccc gcggatgccg
     8341 agggtggcga cccgcgatgt ctccggcgcc ttgcggctgc cgaagccgaa catcgacgtg
     8401 agtccgccca gcgcgttgcc cgacggggca gtctcgaccg tggactggaa gcgcaggtgg
     8461 aacgcccgaa cccagccgcg ctgcgcgccg gactcgacct gggtccagcc gccctggcgc
     8521 gaaagcacct tgacggggga atccgcggcc aggggagcga gcgtccgcga atcgggcgcg
     8581 ggacgctcct tgagatccgt ggcccggttg gtggacgcct gctcctgggc ataggcagtg
     8641 ccgaccagcg gcaggcaagc caggagcagc aggagaatca gtcggtgcgg catggccgcg
     8701 atgataggtc gcccgggcgc aacattcaat gcaatccttc cgtccgcgct tccgtcagcg
     8761 cacgaggacg gtcgcgaagc ccaggagcat tcccatgctt gccacgtcgg tggccgtggt
     8821 gacgatgatg ctcgatgccg tggccgggtc cgcgccgagc cgcttcagcg ccagcggtac
     8881 cagcgccccg gagactccgc tcacggcgca gctgcccatc atcgcgagga acacggccag
     8941 cccgagggca agggcatgcg gcgattcctg cgcgcgcgcg agaaagaaca tcgccgcgcc
     9001 gcacagcacg ccggtaaggc cgccgttgga gagcccgagg aagaattcct tcgcgaccag
     9061 cgccctgccc gcgcccgcgc ggatgtcccc gagcgtcatg ccgcgcacgg tcaccgcgag
     9121 cgcctggcaa ccggtgtttc ccgactgccc cgcgagcacg ggcaggaagg tcgcgaggat
     9181 caccagccgg tccaccgtgc cctggaacat cgccacgacg ccccccgcgg cgaacgccgt
     9241 caggaggttg agttgcagcc acgggtggcg aaacctgaag gcgcgaccga gcgtggtgtt
     9301 gacgcgctcc tccttttcca cgcccaccat cgagccgggc tgcgcggtga tctcgaacgc
     9361 ctgctcctcg aagatcgccg ccccgcgcac caggcccaaa agccggcccg agtcgtcgca
     9421 caccgggtac acggggtaat gccggttgag ggtgagcttc atcgcgtccg acagcggcgt
     9481 gtccgggcgg aagaagaacg cgtccctgag catcacgtcg ccgagcttcg cgccgtgctc
     9541 gttgaacagc aggtcgcgca tcgtgacgat gccgaggagc cttccctcgc cgtccgtgac
     9601 atagccgtag gtgacgaacg ccgtgcgcac gacggcacgc agctgctcga tcgcctcgcc
     9661 cacgctcatg cccggcgaga agacggcgta ggccggttcc atgaggcgcc cgatggcgtc
     9721 ccgcgaatag cgggcattgc gcatccactg cttcgcgttt tccggggcgg ccgcgcgcac
     9781 gatcgcggcg agcgcgcctt ccggcagttc cgccagcacg tcctgcgcgc ggccggggtt
     9841 cagctcggcg agcagttcca ccgcccgcgc cggttcctcc gcggcgagca tccgggccgc
     9901 ctcgcgcggc ggctgggatt tcaggcttgc gcccggggtc tggcgcgctt cggggatctg
     9961 ctggctcacg gcgtgctccg ggttgcggaa gtgccgcgat tctagcccgt gcgcgcccta
    10021 gagccccagc cgttgccaga tggtggtggc gggccccgcc tggttgagcg tgtagaagtg
    10081 caatccgggg gcgccgaggg tgagcaggcg gtcgcagagc tccgtcacca cgtcgaggcc
    10141 gaacgccttg atcgatgccg tgtcgtcgcc gaagctctcc aggcgcaggc ggatccagcg
    10201 cgggatctcg gcgccgcatg cgtcggaaaa gcgcgcgagc tgcgtgaaat tgttgatcgg
    10261 catgacgccc ggcacgatgg gcacgtccac gccgagggcc tccaccgatt ccacgaacct
    10321 gacgtaggcg tcggcgttgt agaagtactg ggtaatggcg gagttggccc cggcctcgat
    10381 cttcgccttg aagtgcgcga ggtcgtcggc cgggcggcgc gcctgcggat ggaattccgg
    10441 gtaggcggcc acctcgatgt ggaaccagtc gccggtgtgg ttgcgcacga aaccgaccag
    10501 ttcggcggca tagcgaaact cgcccgccac cgccatgccc gaaggcaggt cgccgcgcag
    10561 gcagacaagg cgccggatac ccgcggcgcg atattggtcg aggagggccg cgatcgtctc
    10621 gcgtttcgag ccgatgcacg agatgtgcgg tgccgccacc tggccctcgc ggtggatctc
    10681 gaggatggtg cgcagcgtgc cttcctgcgt cgacccgccc gccccgtagg tgcaggacat
    10741 gaaggcgggc ttgagctgcg cgagctggag gcgcgctttg cgcagcttct cgtcgccctc
    10801 cggcgtacgc ggcgggaaga actcgaagct gaacagtttc ggccggtttc gttgcgtctg
    10861 catgggtgca ttcgggggcg cgggcccggc ccgcgccccg cctcgctagt agcggtagag
    10921 ctcggccttg tacgggcctt ccctggccac accgatatag cgcgcctgct cgtcggtaag
    10981 ctcggtgagc tgcgcggtca gcttcttcag ttgcaggcgc gccaccttct cgtcgaggtg
    11041 cttgggcagc acgtacacgc cgatcgggta cttgtccgtg tggccgtaaa gctcgatctg
    11101 cgcgagggtc tggttggcga aggacgagct catcacgtag ctcgggtggc ccgtgccgca
    11161 gcccaggttc accaggcgcc cctcggcgag caggatgatg cgcttgccgt ccgggaagat
    11221 gatgtggtcc acctgcggct tgatgttttc ccacttgtac ttcttgaggc tcgcgacgtc
    11281 gatctcgttg tcgaagtggc cgatgttgca cacgatcgcc tggttcttca tgcgcgacat
    11341 gtgctcgtgc gtgatgacgt ggaaattgcc cgtggcggtg acgaagatgt cggccttgtc
    11401 ggcggcatag tccatcgtca cgacgcgata gccttccatc gaggcctgca acgcgcagat
    11461 cgggtcgatc tcggtcaccc atacctgcgc cgagagcg
//
LOCUS       DASUEV010000065        12903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1271114, whole genome shotgun sequence.
ACCESSION   DASUEV010000065 DASUEV010000000
VERSION     DASUEV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12903
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271114"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFV55_02020"
     CDS             complement(<1..467)
                     /locus_tag="VFV55_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEX4943097.1"
                     /translation="MSKILLSLPKGERVGIAFSGGLDTSAALAWMRHHGAIPYAYTAD
                     LAQPDEVDYNDITRRAKLYGAEQARLVDCREALVHEGLVALQCGAFHIATAGRTYFNT
                     TPLGRAVTGTVLVKAMQEDGVNVWGDGSTYKGNDIERFYRYGLLANPQLRIYKP"
     gene            complement(464..1381)
                     /gene="gnd"
                     /locus_tag="VFV55_02025"
     CDS             complement(464..1381)
                     /gene="gnd"
                     /locus_tag="VFV55_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX4943098.1"
                     /translation="MQIGLVGLGRMGANITRRLCRSDIHVVGFDIGPAAAEALAGEPG
                     FEAAFSVDALLLALKAPRTVWCMLPAGAATRQMLDDLLARLAPGDCVVDGANGHYRET
                     MARAARFEAAGIGFVDAGVSGGIWGLANGYSLMLGGPEGDIAALDRVWRALAPQPRQW
                     LRCGPAGAGHFVKMIHNGIEYGLMQAYAEGFALMSDHPDFDLDLAAIASTWMQGSVVR
                     SWLLELTRDLLAEDARLADVAPVVADSGEGRWTAQEAIDRGVPAPVITAALMQRFASQ
                     GRDDFAARVLATMRNRFGGHAIPNRMSPR"
     gene            1497..2402
                     /locus_tag="VFV55_02030"
     CDS             1497..2402
                     /locus_tag="VFV55_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX4943099.1"
                     /translation="MRLALLAFSALVAFPWTALAQPATGIAPPPAARQYLWEVASLTN
                     RIYLYGTVHAGKRSFYPLAPSVEKAFADSKVLAVEADITDARAMAIGASAMLFTPPDS
                     LPKHVPAPMYERFRRQLDRFGLPEPQAAQMKPFIASSMLAFAEWGRQGYEQQYGVDLH
                     LITRAREAKMSIVELEGAQVQASLMDSLTEKESLQAFEGTVAALESGLTRDQITGLVN
                     AWQSGDPDLLLEVLRAYNESVPGARELEEKFIWSRHGEMAKKIEGWLLDGRDRVFVAV
                     GAVHLAGPRGLVAMLRERGYTVRQL"
     gene            2399..4072
                     /locus_tag="VFV55_02035"
     CDS             2399..4072
                     /locus_tag="VFV55_02035"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4943100.1"
                     /translation="MSDLRQGGRILVEQLLVHGADTAFCVPGESFLPVLDAMYEHRER
                     IKLVVCRHETGAANMAEAHAKLTGRPGICIVTRGPGATQASVGVHTAFQDSTPMILLV
                     GDVGSAFRDREAFQEVDFAAMFAPLAKWAARIDSVERIPEYLSRAFTVAMSGRRGPVV
                     LALPEDVLSATAAVADARMYAPAHAEAGAGEIERLHTLIAAARRPLVVLGGSGWSKDA
                     CEDMRRFIEANHLPVACAFRFQDLFDNRHGNYVGDVGIGINPILAERVRTADVLLVVG
                     ARMGEMTTSGYTLLDSPVPRQKLVHVMSGAEELGRVYQAELAILAGMGRFARAAAAMA
                     PVDASAWKNGVAEARADYIAWTARREIPGKVQMWDIVDWLDKHLPEDAILCNGAGNYA
                     TWLHRFHRYTGFRTQLAPTSGAMGYGVPAAIAAKIAEPGRVVVAFAGDGCFLMAGQEL
                     ATAVQYGAAVIIVVVNNGMYGTIRMHQEKQYPGRVYGSDLVNPQFASYARSFGAVGEI
                     VEETAQFGPAFERCLASGKPAVIEVRIDPQAITPNTTLDAIRAAASVKK"
     gene            complement(4270..4803)
                     /locus_tag="VFV55_02040"
     CDS             complement(4270..4803)
                     /locus_tag="VFV55_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4943101.1"
                     /translation="MIARGGIAMIARAWLGLALLLLGLAAAAAPGATDDPRPFDARSL
                     EAIRRTHAGRPFVLSLWSVHCEPCAREMPLWRELRARYPGVPVVLVATDGPPELDRIR
                     TFLRRNDPGPVEHWRYADEFEERIRFAIDPKWRGELPRTYFHDAAHKVEAVSGMPDAK
                     WIENWFSRMARQGPSAR"
     gene            complement(4800..5960)
                     /locus_tag="VFV55_02045"
     CDS             complement(4800..5960)
                     /locus_tag="VFV55_02045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX4943102.1"
                     /translation="MKRIGGALLGCGFALAVAAQHEGHGAAKGGGSRPELGASAAFDS
                     RGQLWAVYKEGGHVMLRRSPDQGRTWAPARAVNAKPEPVAADGDARPKLAVGPAGDIY
                     VTWTQPLARPYTGFIRFARSVDGGDTFAEPLTVHADRQEITHRFDAIAVTPDAKVFIA
                     WIDKRDRTDEGFRGASLYYAVSDDRGASFRGDYRAAQHSCECCRIALVPQDDGSVLAL
                     WRHVFEPNVRDHAWARLGADGAAGEVRRATFDDWRIDACPHHGPSLAIDAAGRRHAVW
                     FSGAPGKEGVFYGRLGERGPEGKRRVGGDAAEHADIAVAGTRVAIAWKEFDGQRSTLR
                     ALRSDDAGGTWREVSLASSGGPTDQPKVLARGETFHVLWNARDEPLRVVALP"
     gene            complement(5969..8116)
                     /locus_tag="VFV55_02050"
     CDS             complement(5969..8116)
                     /locus_tag="VFV55_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4943103.1"
                     /translation="MGNLHSRRTFVINLCMAAAGTGLTVQAGADEVVAFHSTETRVVV
                     TGTREEGLLSETPASVGVIDEKTVRQDRPTHPAQVMGQVPGVAVAVTNGEGHTTAIRQ
                     PFTTNPVYLFLEDGIPIRSTGFFNHNALYETNIPVAGGIEVTRGPGTALYGSDAIGGI
                     VNVLTRTPPTQREFHASPDFGSFGWRRLIAGGGTSLDHDAWRADLNVTHTDGWRDKTA
                     YDRQSGVARWDHSFDSGAMAKTVLSYSRIDQETGANSPLVESDYRNNPTRNYLPIAYR
                     KVNALRLSTAWEQEWGGTLISVTPYFRDDGMELLASFMLNSDPTVASTSNRSYGVLAK
                     WRRNFPGALRPSLIAGVDVDISPGSREEDRLDVSPTGSGAQRVFSTYAVGPRVYDYAV
                     TFQGVSPYLHGEISPLDRLRVTAGVRFDRLEYDFDNHREGAPIAVPGAFPGLRYYGQA
                     DDTKKTFTHTSPKLGATFALAADTHLYASYNHGFRAPSESQVFRPSAATSSAAAQALV
                     RSSLDLKPIRAQQAELGVRGQAGPVHYDVVAYRLDKRDDILTFRDTLTNFTQSVNAGR
                     TRHEGVEVGLGASFTRQLSIDVSWSRARHKYEEWVTSGGNFTGKEIESAPRDLGNVRL
                     NWAPAADARVTLEWTRIGKYWMDAANTARYPGHDLFNARANWPLTKRIALFGSITNIA
                     DKRHAASASITSGTPVFSPGLPRSYNAGLELKW"
     gene            complement(8182..8556)
                     /locus_tag="VFV55_02055"
     CDS             complement(8182..8556)
                     /locus_tag="VFV55_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX4943104.1"
                     /translation="MFRRRLIVVVTLLCVAWATLWPLVSAAHSLLSDTQVPLCHQAGQ
                     AVPIGIGQVPDGDGAPSGGAKVHCPLCIMAFFAAFEPPLEAPPALFFTTGVALQAHCA
                     AVPAGIEVPLPQGRAPPLPLAS"
     gene            8650..11295
                     /gene="mdeB"
                     /locus_tag="VFV55_02060"
     CDS             8650..11295
                     /gene="mdeB"
                     /locus_tag="VFV55_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HEX4943105.1"
                     /translation="MDSSIPKPVGDPRDPDADPLETREWIEALDALIASEGNERATFL
                     LRRLLQHARTRRVPLPQVLATPYVNTIGLDEQPPYQGNLEIEARLSALVRWNALAMVV
                     RANRTAAELGGHIASYASAADLFEVGFNHFFRAGQEGDIVYFQPHSAPGVYARAYLEG
                     RLDEGHLEHYRRETGGEGLSSYPHPWLMPGFWQFPTGSMGLGPLFAIYQARFMRYLEH
                     RGHLATAGRKVWAVVGDGEMDEPEALAGLSVAARERLDNLVLVVNCNLQRLDGPVRGN
                     GSIIQELEGLFAGAGWNVIKLLWGGDWDPLFARDTHQLLEKRFAETVDGEFQTYAATD
                     GRFNREHFFNRYPELQSLAAHLSDDDIDRLRRGGHDPVKIFSAYWHAVHHAGQPTVIL
                     AQTKKGYGMGTAGQGRMTTHSQKKLDTEELLAFRERFALPLTDAQVEDCAFVRPPRES
                     PEMAYLHARRGKLGGYLPARSTQCEPLATPAAAAFAKFALEAGGKEMSTTVALVRIVG
                     ALLRDPVLGRRIVPIIADEARTFGMADLFRQIGIYSPLGQLYEPEDHEQLSFYKEAVD
                     GQILEEGINEAGAIASWVAAATSYSAHGYAMLPMYIYYSMFGFQRVGDAIWAAADSRS
                     RGFLVGATAGRTTLAGEGLQHQDGTSHLVASTIPNCRAYDPAFAYELAVIVEDGMRRM
                     LSAREDVFYYVTVMNESYAQPSLPAGAAEGILRGMYRVHQAAGAKVRLLGAGAILPEA
                     LAAAELLERDWQVPAEAYSVTSFTELRREAMAASREARLGGKPATPWIETQLPQTGLP
                     VVAASDYVSAVADLIRPWVLDPFLALGTDGFGRSDTRRELRRFFEVDRHAIAVAALHA
                     LEDPRKGEAMRRYGIMEGNPPPWRR"
     gene            complement(11316..11807)
                     /locus_tag="VFV55_02065"
     CDS             complement(11316..11807)
                     /locus_tag="VFV55_02065"
                     /inference="COORDINATES: protein motif:HMM:NF045536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070088 - polyhydroxyalkanoate granule
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate granule-associated phasin"
                     /protein_id="HEX4943106.1"
                     /translation="MTRRVPRYSYLAPFMQWSDLAMKTGEMMLASAQVIGHRTGRMAR
                     AGASPSAGDWREFALMGQEKLDAGAQSGQAMASHMMTMNPQLGAQAFADMVRSTSALM
                     SLVSSQTPAQMFARQAELARVLERSAESLADASASAVRLAHHALTPIHASATANAKRL
                     ARR"
     gene            complement(11903..12463)
                     /locus_tag="VFV55_02070"
     CDS             complement(11903..12463)
                     /locus_tag="VFV55_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX4943107.1"
                     /translation="MNIAIDPRRCALVLVDYQERLLPAIHRGAEVVAEAARLADCARA
                     LDMRVIGTEQNPQGLGPNARVIRSRCGTTVAKMHFDGCADGLADVLRAPGRTVPSDVV
                     IAGCETHVCLMQTAHGLQRAGFGVWVAANACGTRFPVDHDLALGRLRQSGAVLASVEM
                     IAFEWLRTCGHERFKAVLSVLKAPRA"
     gene            complement(12479..>12903)
                     /locus_tag="VFV55_02075"
     CDS             complement(12479..>12903)
                     /locus_tag="VFV55_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX4943108.1"
                     /translation="GVRSTATIVSEQFAMPAALQWKGVADIITKGLTSLPHSAVVSMV
                     VAAACALAIEVARIVTKARFPLSSVSIGLGVVLPPEATFAMWAGAMIFWWAGRANATP
                     GTKGHRFWVEGCEPICAGLISGAALVGIGNAIVNVLLG"
BASE COUNT         1976 a         4390 c         4544 g         1993 t
ORIGIN      
        1 ggcttgtaga tgcgcagctg cgggttggcg agcagcccgt agcggtagaa gcgctcgatg
       61 tcgttgccct tgtaggtcga gccgtcgccc cagacgttga cgccgtcttc ctgcatcgcc
      121 ttcaccagga cggtgccggt cacggcgcga ccgagcggcg tggtgttgaa gtacgtgcgc
      181 cccgccgtgg cgatgtggaa ggcgccgcat tgcagggcga cgaggccttc gtggacaagc
      241 gcctcgcggc aatcgacgag gcgggcctgc tcggcgccgt agagcttcgc gcggcgcgtg
      301 atgtcgttgt agtccacctc gtcgggctgc gcgaggtcgg cggtgtaggc gtagggaatg
      361 gcgccgtggt ggcgcatcca ggcgagggcg gcagaggtgt cgaggccgcc ggaaaaggcg
      421 attccgactc gctcgccctt ggggagtgac aggaggattt tgctcatcgt ggcgacattc
      481 tattcggaat cgcgtggccg ccgaagcggt tcctcatcgt ggcgagcacg cgcgcggcga
      541 aatcgtcgcg cccctggctc gcgaaccgct gcatgagcgc cgcggtgatg acgggcgcgg
      601 gcacgccgcg gtcgatcgcc tcctgcgcgg tccagcggcc ctcgccggag tcggccacca
      661 ccggggccac atccgccagc cgcgcgtcct cggcgaggag gtcgcgcgtg agctccagga
      721 gccacgagcg cacgacgctg ccctgcatcc aggtgctcgc gatcgcggca aggtcgaggt
      781 cgaaatcggg gtggtcgctc atcagggcga agccctcggc ataggcctgc atgaggccgt
      841 actcgatgcc gttgtggatc atcttcacga aatggcctgc gcccgcgggc ccgcagcgca
      901 gccactgcct gggctgcggc gcgagcgcgc gccagacccg gtcgagggcg gcgatgtcgc
      961 cctcggggcc accgagcatc agcgaatagc cgttggcgag cccccagatg ccgcccgaaa
     1021 cgccggcatc cacgaacccg atgccggccg cctcgaagcg cgcggcgcgc gccatcgtct
     1081 cgcggtagtg gccgttggcc ccgtccacga cgcagtcgcc gggcgcgagc ctggcgagga
     1141 ggtcgtcgag catctgccgc gtggctgcgc ccgccggcag catgcaccag acggtgcgcg
     1201 gcgccttcag cgcgaggagg agcgcatcga cggaaaaggc ggcctcgaat cccggttcgc
     1261 cggcgagggc ctcggcggca gcgggcccga tgtcaaagcc cacgacgtgg atatccgacc
     1321 ggcacaggcg ccgcgtgatg tttgcgccca tcctgccgag accgactagg ccgatttgca
     1381 tgggatcatc cggttggttg ctcgcgagtt gcaagagaat tgcgacagca taccgccgcg
     1441 gcccgtgacc cggcgtgggc tagaatggaa gactgccttc cgacgccgga aatttcatgc
     1501 gccttgcgct cctcgccttc tccgcactcg ttgcctttcc ctggaccgcg ctcgcgcaac
     1561 ccgccaccgg catcgcgccg ccccccgccg cccgccagta cctgtgggag gtggcttccc
     1621 tcaccaaccg catctacctg tacggcaccg tgcacgcggg caagaggagc ttctatccgc
     1681 ttgcgccatc ggtggagaaa gccttcgcgg attcgaaggt gctcgccgtc gaggccgaca
     1741 tcaccgacgc ccgggcgatg gcaatcggcg catccgcgat gctgttcacg ccgcccgact
     1801 cgctgccgaa gcacgttccg gcgcccatgt acgagcgctt ccgcaggcag ctcgaccggt
     1861 tcggcctgcc ggagccgcag gcggcgcaga tgaaaccctt catcgcctcg tcgatgctgg
     1921 cgttcgcgga atggggccgg cagggttacg agcagcagta tggcgtcgat ctccacctca
     1981 tcacccgtgc ccgcgaggcg aagatgagca tcgtcgagct cgaaggcgcg caggttcagg
     2041 cgtccctcat ggactccctc acggagaagg agagcctgca ggccttcgag ggcaccgtcg
     2101 ccgcgctgga aagcggcctc acgcgcgacc agatcacggg cctggtgaat gcctggcagt
     2161 cgggcgaccc ggacctgctg ctggaagtgc tgcgcgccta caacgagagc gtgccgggcg
     2221 ctagggagct ggaggagaag ttcatctggt cgcgccatgg cgaaatggcg aagaagatcg
     2281 aaggctggct gctcgacggg cgcgatcggg tgttcgtggc cgtgggtgcc gtccacctgg
     2341 ccggtccgcg cggtctggtg gcgatgctgc gcgagcgcgg ctacacggtg aggcagttat
     2401 gagcgacttg cgtcagggtg ggcgcattct tgtcgagcag ctcctggttc acggggccga
     2461 cacggccttc tgcgtccccg gcgagagctt cctgccggtg ctggacgcga tgtacgagca
     2521 ccgcgagcgg atcaagctgg tggtctgccg ccacgagacg ggcgcggcaa acatggcgga
     2581 ggcgcacgcc aagctcaccg gccgcccggg gatctgcatc gtgacgcgcg gcccgggcgc
     2641 cacccaggcc tcggtgggcg tgcacaccgc cttccaggat tcgacgccga tgatcctcct
     2701 tgtcggcgac gtcggctccg ctttccgcga ccgcgaggca ttccaggaag tggatttcgc
     2761 cgccatgttc gcgccgctcg ccaagtgggc ggcacgcatc gattcggtcg agcgcatccc
     2821 cgagtacctg agccgcgcct tcaccgtggc catgtcgggc cggcgcggac cggtggtgct
     2881 ggcacttccc gaggatgtgc tgtccgcgac cgcggcggtg gccgatgcgc gcatgtatgc
     2941 gccggcgcac gccgaggccg gtgccggcga gatcgagcgc ctgcacacgc tcatcgccgc
     3001 ggccaggcgg cccctcgtgg tgctgggcgg cagcgggtgg tcgaaggacg cgtgcgagga
     3061 catgcgccgg ttcatcgaag ccaatcacct cccggtcgcc tgcgccttcc gcttccagga
     3121 tctcttcgac aaccgccacg gcaactacgt gggcgacgtg ggcatcggca tcaaccccat
     3181 cctcgcggag cgcgtgcgca ccgcggacgt gctcctggtg gtgggcgcgc gcatgggcga
     3241 gatgacgacg agcggctaca cgcttctcga ctcgcccgtg ccgcggcaga agctggtgca
     3301 cgtgatgagc ggcgcggagg agctgggccg cgtctaccag gccgagctcg ccattctcgc
     3361 gggcatgggc cggttcgcgc gcgccgcggc cgcgatggcg cccgtcgatg cctcggcctg
     3421 gaagaacggc gtggcggagg cacgggcgga ttacatcgcc tggaccgcgc gccgcgagat
     3481 cccgggcaag gtgcagatgt gggacatcgt ggactggctg gacaagcacc tgccggaaga
     3541 cgcgatcctc tgcaacggcg ccggcaacta cgcgacctgg ctgcaccggt tccaccgcta
     3601 cacgggattt cgcacgcagc tggcgcccac ctcgggcgcc atgggctacg gcgtgcctgc
     3661 ggcgatcgcc gcgaagatcg cggagcccgg tcgcgtcgtg gttgccttcg ccggcgatgg
     3721 ctgctttctc atggcggggc aggagctcgc caccgcggtg cagtacggcg ccgcggtgat
     3781 catcgtcgtg gtgaacaacg gcatgtacgg caccatccgc atgcaccagg aaaagcaata
     3841 cccggggagg gtgtacggca gcgacctcgt gaacccgcag ttcgcctcct acgcccggtc
     3901 cttcggagcg gtaggcgaga tcgtggagga gaccgcgcag ttcgggcctg cattcgagcg
     3961 ttgcctggct tccggcaagc cggcggtgat cgaggtgcgc atcgatccgc aggcgatcac
     4021 gcccaatacc acgctcgatg cgattcgcgc ggcggcttcg gtgaagaaat gagcggtggg
     4081 gagttcagtg accgcaaatc ctggaacacg gatgaacacg gataacgcct gagtgatgta
     4141 tccgtgcccg aagttgacgc cgatcgaaac ggccacgctt ccactcaatg aattatccgt
     4201 gttcatccgt gttcatccgt gtccccgcct ttgggcaact atgtgcacca cgtctcctcc
     4261 cacgccccgt catcgcgcgg aaggcccctg ccgggccatg cggctgaacc agttctcgat
     4321 ccatttcgcg tcgggcatcc ccgaaaccgc ttcgaccttg tgcgccgcgt cgtgaaagta
     4381 cgtgcgcggc agctcgccac gccacttcgg gtcgatcgcg aagcggatcc gttcctcgaa
     4441 ttcgtcggcg tagcgccagt gttcgaccgg gccgggatcg ttgcgccgca ggaaggtcct
     4501 gatgcgatcg agttccggcg ggccgtccgt ggcgacgagc accaccggca ccccgggata
     4561 gcgcgcccgc aactcgcgcc acagtggcat ctcgcgcgcg cacggctcgc agtgcaccga
     4621 ccatagcgac agcacgaacg gccggccggc atgggtgcgg cgaatggcct ccaggctgcg
     4681 cgcgtcgaag gggcggggat cgtccgtggc gcccggcgcc gcggccgcgg ccaaccccag
     4741 cagcaggagg gcgaggccaa gccaggcgcg cgcgatcatg gcaatgccac cacgcgcgat
     4801 catggcaatg ccaccacgcg cagcggctcg tcgcgcgcgt tccagaggac gtggaacgtc
     4861 tcgccgcgcg caagcacctt cggctggtcc gtgggtccgc cgctcgaggc gaggctcact
     4921 tcccgccacg tcccgccggc gtcgtcggag cgcagcgccc gaagcgtgct gcgctgcccg
     4981 tcgaactcct tccaggcgat tgccacgcgc gtgcccgcga cggcgatgtc cgcatgctcg
     5041 gccgcatcgc cgcccacgcg ccgcttccct tcggggccgc gctcgcccag ccggccgtag
     5101 aacacgcctt ccttgccggg cgcgccgctg aaccagacgg catgccttct gcccgccgcg
     5161 tcgatcgcga gggacggccc gtggtgcgga caggcatcga tccgccagtc gtcgaaagtc
     5221 gcgcgccgga cttcgcctgc cgcgccgtcg gcgccgagcc gcgcccaggc gtggtcgcgg
     5281 acgttgggct cgaagacgtg gcgccagagc gcgagtacgc tgccgtcgtc ctgtggcacg
     5341 agcgcgatgc ggcagcactc gcaactgtgc tgcgccgcgc ggtagtcgcc gcggaacgag
     5401 gcgccgcgat cgtcggacac ggcgtagtac agcgacgcgc cgcggaagcc ttcatccgtc
     5461 cgatcgcgct tgtcgatcca cgcgatgaag accttcgcgt cgggcgtgac ggcgatcgcg
     5521 tcgaagcggt gggtgatttc ctggcggtcg gcgtgcaccg tgagcggctc ggcgaacgtg
     5581 tcgccgccat cgacggagcg cgcgaagcgg atgaagccgg tgtagggcct ggccagcggc
     5641 tgcgtccagg tcacgtagat gtcgcccgcg gggccgacag ccagcttcgg gcgagcgtcg
     5701 ccgtcggcgg ccaccggctc cggcttcgca ttgaccgcgc gcgcgggcgc ccaggtgcgc
     5761 ccctggtcgg gcgagcgccg cagcatcacg tggccgcctt ccttgtacac ggcccagagt
     5821 tgcccccgcg agtcgaaggc ggcgctcgcg cccagctcgg ggcgggaacc gccacccttc
     5881 gctgcgccat ggccttcgtg ctgcgcggcg accgccagcg cgaatccgca accgagcagc
     5941 gcgccaccga tccttttcat cgtgaccgct accatttgag ttccaggccg gcgttgtagg
     6001 accgcggcag tccaggagaa aaaaccggcg tgccggaggt gatcgaggcg ctggccgcgt
     6061 ggcgcttgtc cgcgatattg gtgatgctgc cgaaaagcgc gatgcgcttc gtgagcggcc
     6121 agttcgcccg ggcgttgaac aggtcgtgcc ccggataccg cgcggtgttg gctgcgtcca
     6181 tccagtattt gccgatcctt gtccactcca gggtcacgcg agcgtccgcg gcgggcgccc
     6241 agttcaggcg cacgttgccg agatcgcgcg gcgccgattc gatctccttg ccggtgaagt
     6301 tgccgcccga tgtcacccac tcctcgtact tgtggcgcgc gcgcgaccac gagacgtcga
     6361 tcgagagctg gcgggtgaag ctggccccca ggcccacctc gacgccctcg tgccgtgtgc
     6421 gcccggcgtt caccgactgg gtgaagttgg tcagggtgtc gcgaaaggtg aggatatcgt
     6481 cgcgcttgtc cagtcgatag gccacgacgt cgtagtggac cgggcccgcc tggccgcgca
     6541 cgccaagctc cgcctgctgc gcgcggatgg gcttgaggtc cagcgacgag cgcacgagcg
     6601 cctgcgccgc ggcgctcgag gtcgccgcgg aaggccggaa gacctggctc tccgagggcg
     6661 cgcgaaagcc gtggttgtac gaggcataca ggtgcgtgtc cgcggcgagc gcaaaggtgg
     6721 cgcccagctt cgggctcgtg tgggtgaagg tcttcttcgt gtcgtcggcc tggccgtagt
     6781 agcgcagccc cggaaacgcc cccggcacgg cgatgggtgc gccctcacga tgattgtcga
     6841 agtcgtactc cagccggtcg aaacgcacgc cggccgtcac tcgcaggcga tcgagcgggg
     6901 agatctcgcc gtgcaggtac ggcgagacac cctggaaagt cacggcgtag tcgtacaccc
     6961 tggggccgac ggcgtaggtg ctgaatacgc gttgcgcgcc gcttccggtg gggctcacgt
     7021 cgaggcggtc ctcctcccgg cttccggggc tgatgtccac gtcgacgccg gcgatcaggc
     7081 tcgggcgaag cgcgcccgga aaattccgcc gccacttggc cagcacgccg taggaccggt
     7141 tgctcgtgga ggctacggtg ggatcggaat tcagcatgaa agaggcgaga agctccattc
     7201 cgtcatcgcg gaagtagggt gttaccgaaa tgagcgtgcc cccccattcc tgctcccacg
     7261 ccgtggacag gcgcaacgcg ttcaccttgc gataggcgat gggcaggtag ttgcgcgtcg
     7321 gattgttgcg gtagtcgctt tcgacgagcg gggagttggc gccggtttcc tggtcgatgc
     7381 gcgagtaact gagcacggtc ttggccatgg cgccggagtc gaatgaatgg tcccagcgtg
     7441 cgacgccgct ctggcggtcg taggcggtct tgtcgcgcca gccatcggtg tgcgtcacgt
     7501 tgaggtccgc gcgccaggcg tcgtgatcga gggaggtgcc gcccccggcg atgagccgcc
     7561 gccagccgaa gctgccgaag tccggcgacg catggaactc gcgctgcgtc ggcggcgtgc
     7621 gcgtgagcac attgacgatg ccgccgatgg catccgaacc atagagcgcc gtccccgggc
     7681 cgcgggtcac ctcgatgccg cccgccaccg ggatgttggt ttcgtagagc gcgttgtggt
     7741 tgaagaagcc cgtggagcgg atcgggatgc cgtcctcgag gaagaggtag acggggttgg
     7801 tggtgaaggg ctggcggatg gcggtggtgt ggccctcgcc gttggtgacg gccacggcca
     7861 cgcccggcac ctgccccatc acctgggcgg gatgcgtggg ccggtcctgg cgcacggtct
     7921 tttcgtcgat gacgccgacc gatgcgggag tctcggacag gaggccctct tcacgcgtgc
     7981 cggtgacgac gacgcgggtt tcggtcgaat ggaaggcgac gacttcgtcg gccccggctt
     8041 ggacggtcaa tcccgtcccg gccgcggcca tgcataggtt gatgacgaac gttcttcggg
     8101 aatgaaggtt tcccatggaa ttctccctgg caatacgaac cgccacgcgc gcgaatgccg
     8161 cgcggtggaa tgacgactgc gtcagctcgc gaggggcagg gggggcgcgc ggccctgggg
     8221 aagggggact tcgatgccgg ccggcactgc ggcgcaatgg gcttgcaacg cgacgccggt
     8281 ggtgaagaac aacgcgggcg gcgcctcgag cggcggttcg aacgccgcga agaacgccat
     8341 gatgcaaagg gggcagtgga ccttggcgcc gcccgaaggg gcgccgtcgc cgtccggcac
     8401 ctggccgatg ccgatcggga ccgcctggcc ggcctggtgg cacaacggca cctgcgtatc
     8461 ggagagcagc gagtgcgccg ccgacaccaa tggccacagc gtcgcccagg cgacgcacag
     8521 gagggtgaca acgacgatga gccggcggcg gaacatggcg atattttatg tgcccccgga
     8581 tgcgcgtgcc tgcggcgaat cgccgcaggc accctgccac ggtcgccgca cgggccgtac
     8641 aatcggcgca tggactcatc catcccgaag cccgtcggcg acccccgcga tccggacgcc
     8701 gatcctctcg aaacgcgcga gtggatcgag gcgctcgacg cgttgatcgc ctccgaaggc
     8761 aacgaacgcg cgacgttcct gctgcgccgc ctgctgcagc acgcgcgcac acggcgcgtg
     8821 ccgttgccgc aggtactcgc gaccccctac gtgaacacca tcgggctcga cgagcagccc
     8881 ccgtaccagg gcaacctcga gatcgaggcg cgcctctcgg ccctggtgcg ctggaacgcg
     8941 ctcgcgatgg tggtgcgcgc caaccgcacg gccgcggagc tgggcgggca cattgcgagc
     9001 tacgcctccg ccgccgatct cttcgaggtg ggcttcaacc acttcttccg cgccgggcag
     9061 gaaggcgaca tcgtctattt ccagccgcac tccgcgccgg gcgtgtacgc gcgcgcctac
     9121 ctcgagggcc ggctggacga agggcacctg gagcactatc gccgcgagac gggcggcgag
     9181 gggctgagtt cgtacccgca cccgtggttg atgcccggtt tctggcaatt cccgaccggc
     9241 tccatgggcc tggggccgct cttcgcgatc taccaggcgc gcttcatgcg ctacctcgag
     9301 caccgcggcc acctcgccac ggccggccgc aaggtctggg ccgtcgtcgg cgacggcgag
     9361 atggacgagc cggaggcgct cgcgggcctg tccgtggccg cgcgcgagcg gctcgacaac
     9421 ctggtgctcg tggtcaactg caacctgcag cgcctggacg gcccggtgcg cggcaacggt
     9481 tccatcatcc aggagctgga ggggctcttc gccggcgcgg gctggaacgt catcaagctc
     9541 ctctggggcg gcgattggga cccgctcttc gcgcgcgaca cgcaccagct cctcgagaag
     9601 cgcttcgccg aaacggtcga cggcgagttc cagacctacg ccgcgaccga cgggcgcttc
     9661 aaccgcgagc acttcttcaa caggtatccg gagctgcaga gcctcgcggc gcacctctcg
     9721 gacgacgaca tcgaccgcct gcgccgcggc ggccacgacc cggtgaagat cttctcggcg
     9781 tactggcacg ccgtccacca cgcgggccag cccaccgtca tcctcgcgca gacgaagaag
     9841 ggttacggca tgggcacggc ggggcagggg cgcatgacca cgcattcgca gaagaagctc
     9901 gatacggaag agctcctcgc cttccgcgag cgcttcgccc tgccgctcac cgacgcgcag
     9961 gtggaggact gcgcgttcgt gcgcccgccg cgcgaatccc ccgagatggc ctacctgcac
    10021 gcgcggcgcg ggaagctggg cggctacctg ccggcgcgct cgacccagtg cgaacctctt
    10081 gccacgccgg cggccgccgc gttcgcgaag ttcgcgctcg aggccggagg caaggagatg
    10141 tccaccacgg tcgccctcgt gcgcatcgtg ggcgcgctcc tcagggaccc ggtgctgggc
    10201 cggcgcatcg tgcccatcat cgcagatgag gcgcgcactt tcggcatggc ggacctcttc
    10261 cgccagatcg gcatctactc gccgctgggg cagctctacg agcccgagga ccacgagcag
    10321 ctttcgttct acaaggaggc ggtcgacggc cagatcctcg aggagggcat caacgaggcg
    10381 ggcgccatcg cctcctgggt cgcggcggcc acgagctact cggcgcacgg ctacgcgatg
    10441 ctgccgatgt acatctacta ctcgatgttc gggttccagc gcgtgggcga tgcgatctgg
    10501 gccgcggcgg actcgcgttc gcgcggcttt ctcgttggcg ccacggcggg acggacgaca
    10561 cttgccgggg agggcctgca acaccaggac ggcaccagcc atctcgtcgc ctcgaccatc
    10621 cccaactgcc gcgcgtacga ccccgcgttc gcctatgaac tcgcggtgat cgtcgaagac
    10681 ggcatgcgcc gcatgctttc ggcgcgggag gacgtcttct actacgtgac ggtgatgaac
    10741 gagagttacg cgcagccttc gctgccggcg ggcgcggcgg aagggatcct gcgcggaatg
    10801 taccgggtac accaggctgc gggcgcgaag gtgcggctgc tgggcgcggg cgcgattctc
    10861 cccgaggccc ttgcggcggc ggaactcctc gagcgcgact ggcaggtgcc ggccgaggcg
    10921 tattccgtca ccagtttcac ggagctgcgg cgcgaggcca tggccgcttc acgcgaggcg
    10981 cggctgggag gcaagcccgc gacgccgtgg atcgaaaccc agcttccgca gaccgggctt
    11041 cccgtcgtcg ccgccagcga ttacgtgagc gcggtggcgg acctcatccg gccctgggtg
    11101 ctcgatccgt tcctggccct gggcacggat ggcttcgggc gcagcgacac gcgcagggaa
    11161 ctgcgacgct tcttcgaagt ggatcgccac gccatcgcgg tggcggcgtt gcacgcgctg
    11221 gaagatcccc ggaagggcga ggcgatgcgg cgctacggga tcatggaagg gaacccgccg
    11281 ccctggcggc gctgacgcgg cgccgcaatt tcccgtcaac gcctggcaag ccgtttcgcg
    11341 ttcgcggtgg cgctggcgtg aatgggcgtc agtgcgtgat gcgcaagcct gacggcgctt
    11401 gccgaagcgt ccgccaggct ctcggcggat cgttccagca cccttgccag ttccgcctgg
    11461 cgcgcgaaca tctgcgcggg cgtctggctg gacaccaggg acatcaatgc ggatgtgctg
    11521 cgcaccatgt cggcgaatgc ctgcgcaccc aattgcggat tcatcgtcat catgtgcgag
    11581 gccatcgcct ggccggattg cgcgcccgcg tcgagctttt cctgccccat cagcgcgaat
    11641 tctcgccagt cgcccgcgct gggggaggcg cccgcccgcg ccatgcgccc cgtccgatga
    11701 ccgatgacct gcgccgacgc cagcatcatt tcgccggtct tcatggccag gtcgctccac
    11761 tgcatgaacg gggcgaggta cgaatagcgg ggaacacggc gcgtcatgtc gttccttggt
    11821 tgggaatgcc gggatagcat tccgagattg aaggggaaag cgaaagccat ctgtgcgata
    11881 gggcgcatat accccgatat cgtcaggccc gcggggcctt gagcaccgag agcaccgcct
    11941 tgaagcgctc gtgcccgcag gtgcgcagcc actcgaacgc gatcatctcg acgctcgcga
    12001 gcacggcacc ggactggcgc agacgcccga gcgcgaggtc gtgatccacg gggaatcgcg
    12061 tgccgcaggc gtttgcggcc acccagaccc cgaatccggc gcgctgcagg ccgtgcgcgg
    12121 tctgcatgag gcacacgtgc gtctcgcagc cggcgatcac cacgtcgctg ggcaccgtcc
    12181 gccccggggc gcgcagcacg tccgccaggc cgtcggcgca accgtcgaaa tgcatcttcg
    12241 cgacggtggt cccgcagcgg ctgcggatga cccgcgcgtt gggacccagc ccctgcgggt
    12301 tctgttccgt gccgatcacg cgcatgtcca gcgcgcgcgc gcaatcggcc aggcgcgcgg
    12361 cctcggcgac cacctccgcg ccgcggtgga tggcgggcag cagccgctcc tggtaatcca
    12421 ccagtacgag ggcacatcgg cgcgggtcga ttgcgatgtt catgcgcggc ccttcccgtc
    12481 agcccagcag cacgttgacg atggcgttgc cgatgcccac cagcgccgcc ccggagatga
    12541 ggcccgcgca gatgggctcg caaccctcga cccagaacct gtgccccttc gtgccggggg
    12601 tcgcgttggc ccggcccgcc caccagaaga tcatcgcgcc cgcccacatg gcgaaggtgg
    12661 cctccggcgg caggaccacg cccaggccga tggagacgga ggatagcggg aagcgcgcct
    12721 tggtgacgat gcgcgcgacc tcgatggcca aggcgcacgc agccgcgacg accatggaga
    12781 cgacggcgga gtgcggcagg ctggtgaggc ccttggtgat gatgtcggcc acgcccttcc
    12841 attgcagggc cgcgggcatg gcgaactgtt ccgagacgat ggtcgccgtc gagcgcaccc
    12901 cgt
//
LOCUS       DASUEV010000066         4090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_19566, whole genome shotgun sequence.
ACCESSION   DASUEV010000066 DASUEV010000000
VERSION     DASUEV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19566"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFV55_02080"
     CDS             complement(<1..419)
                     /locus_tag="VFV55_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX4943109.1"
                     /translation="MNAILKPNAGAEYVVADLALSDWGRKEIAIAETEMPGLMAIREE
                     FAASQPLRGARITGSLHMTIQTAVLIETLKALGAEVRWASCNIYSTQDHAAAAIAKGG
                     TAVFAKKGESLAEYWDYTHRIFEWPDNGYSNMILDDGG"
     regulatory      complement(495..606)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            709..1335
                     /locus_tag="VFV55_02085"
     CDS             709..1335
                     /locus_tag="VFV55_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4943110.1"
                     /translation="MNAPLLLGCTGCGSAIVECAFALAGLPLDVEEVDYSPGSPTRDR
                     LLAVNPLGQVPALVLPDGRVLTESLAILHWLDDRAPDAKLVPAKGAESRAAFYRWIVF
                     LVAAIYPTFTYGDEPKKWVADEAGAKMLRESTDRHREALWRQAEAAAGSPWFLGEGRS
                     AIDLYLLAMTRWRPGRQWFEAHTPKLAAIARRAGDLGALAPVLERNFG"
     gene            1417..2628
                     /locus_tag="VFV55_02090"
     CDS             1417..2628
                     /locus_tag="VFV55_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943111.1"
                     /translation="MNRLARSRARLALAVCLAATAVPALAQPKGAPAKTPVANYWMDV
                     ATVNVSMPGMEEMADMPLVGGMMGNFFGGSKMGVMPGKWLDLALYTRQKPAGTDGTHA
                     IPPGQRMGSSLPLLPVAKPQPGGTAPESRDVDQQKPKGRLLLYWGCSETVRAGQPRIL
                     DMATAGPMDFAKFFVGRYAPERGATSQPGRSIWPNETDKQRVPKDSSLLGEHAVSGEG
                     VPQSLRFAIGEKQDFMERMNLAAPGDPKGPLTASWNAVSTARAYFLSAMAGGQDDLIL
                     WSSSEAPEAGIGLMDYLANGQIDQWVREKVLLPASTQQCAIPAGIFANAGGAMLRGIA
                     YGNELNLLHPPRPADARLLAAWNPEWSLRVRVKSTSMTMLGQEAAPARQQKREEPTNP
                     LDILRKGIFGK"
     gene            complement(2636..3793)
                     /locus_tag="VFV55_02095"
     CDS             complement(2636..3793)
                     /locus_tag="VFV55_02095"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HEX4943112.1"
                     /translation="MKVEHPSPAAAGARNRYDLKEFSGAFGDLGTLVPFVIAYLAIVK
                     MDAGGVLLGFGGAMIATGLWYRTPVPVQPMKAIGAAAATHAAGAAITPSIVVGSGLVT
                     ALIWVVLGATGLAKKLAEWIPRPALLGVVLGLGFSFMLEGIRMMSGSAWLAAALLAFA
                     LLAHSRLPTMLLLLAAGVVIALVRQPALAAEISAITPAFRWPAFAWPALSAGDLWAGA
                     VLLALPQLPLTFGNALLAITSENNRLFPDRPVTERGIAISTGLANAWSSAMGGVPVCH
                     GAGGMAGHVKFGARTGGSTVMLGVLLALLALFFSDSVHVLLRLFPEPVLGVILFLAGA
                     ELALSSREAGDERVGRFVLLTTAAFTVVHVGVAVIFGILAYHASKRGWLRP"
     gene            3871..>4090
                     /locus_tag="VFV55_02100"
     CDS             3871..>4090
                     /locus_tag="VFV55_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943113.1"
                     /translation="MRTAPSLFLAASVLAAGCGSMSPADHAQHMGHDAAVADGRQVVD
                     FPEPMRTHTLSNMRDHLAALQEIQQALAE"
BASE COUNT          686 a         1416 c         1397 g          591 t
ORIGIN      
        1 ccgccgtcgt ccaggatcat gttcgagtag ccgttgtccg gccactcgaa gatgcggtgc
       61 gtgtagtccc agtactcggc gagcgactcg cccttcttcg cgaagacggc cgtgccgccc
      121 ttcgcgatgg cggcggcggc atgatcctgc gtcgaataga tgttgcacga ggcccagcgc
      181 acctcggcac cgagggcctt gagcgtctcg atgaggacgg cggtctggat cgtcatgtgc
      241 agcgacccgg tgatgcgggc gccgcgcagg ggctggctgg cggcgaattc ctcgcggatg
      301 gccatgaggc cgggcatctc ggtttcggcg atggcgattt ccttgcggcc ccagtcggaa
      361 agcgcgaggt cggccaccac gtactcggcg cccgcgttcg gtttcaggat ggcgttcatg
      421 ctcagtcact cctcaaaggg tgaccggccg gggcaggagg gggatttcgc tctcgctgcc
      481 gcgggcaggc cacggttcaa cgagcgcggt tcggatgatc tcgaagccct gagcctggca
      541 ggtgcggcgg tccccagcct gctttggggg acctgaatcc ggcctgttgc aacgctcctc
      601 agggacggcg cgaattctac cagaatcccg cgggaagagc ggcgcgatgt ctccggcagc
      661 gggcaccgat cgtcctcgaa gggcgatgga ggcataatcc gttccatcat gaacgcccca
      721 ctgctgctcg gctgcaccgg ctgcggctcg gccatcgtcg aatgcgcctt cgcgctggcc
      781 ggcctgccgc tcgatgtcga ggaggtggac tactcgcccg gcagtccgac gcgcgaccgg
      841 ctcctcgcgg tgaacccgct gggacaggtg cccgcgctcg tgttgcccga cggccgcgtg
      901 ctcaccgaaa gcctcgcgat tctccactgg ctcgacgacc gggccccgga tgcgaagctc
      961 gtgccggcga agggagccga gtcccgcgcg gcgttctacc gctggatcgt gttcctcgtg
     1021 gcggcgatct atcccacgtt cacttacggc gacgaaccca agaagtgggt cgccgacgag
     1081 gcgggcgcga agatgctgcg cgagagcacg gaccgccacc gcgaagcgtt gtggcgccag
     1141 gccgaggcgg ctgcgggcag cccctggttt ctcggcgagg gcaggagcgc gatcgacctc
     1201 tacctcctgg cgatgacgcg atggcgcccc ggccggcagt ggttcgaggc gcacacgccg
     1261 aaactcgccg ccattgcgcg ccgcgcgggc gatctcgggg cgctcgcccc ggtcctcgaa
     1321 cgcaacttcg gctgacgctc gccgggtaga tcttccacgc catcacctgc ccttcccctc
     1381 cccattcaat ccgatttccc tatcgcagga gttgccatga atcgcctcgc tcgaagccgg
     1441 gctcgcctcg ccctggccgt ctgccttgcc gccaccgccg tgcccgcgct tgcacagccg
     1501 aagggcgcac cggcgaagac gcccgtcgcc aattactgga tggatgtcgc gacggtcaac
     1561 gtctcgatgc ccggcatgga ggaaatggcc gacatgccgc tcgtcggcgg catgatgggc
     1621 aatttcttcg gcggctcgaa gatgggcgtg atgccgggca agtggctgga cctcgctctc
     1681 tacacgcgcc agaagcccgc gggcaccgac ggtacgcacg ccatcccgcc cggccagagg
     1741 atgggatcga gcctgccgct gctgcccgtg gcgaagccgc agcccggggg caccgccccc
     1801 gagtcgcggg acgtcgatca gcagaagccc aaggggcggc tgctgctcta ttgggggtgc
     1861 agcgagaccg tgcgggcggg ccagccgcgc atcctcgaca tggccaccgc cgggccgatg
     1921 gatttcgcga agttcttcgt cggccgttat gcgccggagc ggggcgccac gagccagccg
     1981 ggccgttcca tctggcccaa cgagaccgac aagcagcgcg tgccgaagga ttcctcgctg
     2041 ctcggcgagc atgcggtgag cggggagggc gtcccgcaga gccttcggtt cgcaatcggc
     2101 gagaagcagg atttcatgga gcggatgaac ctcgcggcgc ccggcgaccc gaaggggccc
     2161 ctcaccgcaa gctggaacgc cgtgtccacg gcgcgcgcgt atttcctgtc ggcgatggca
     2221 ggcgggcagg acgacctcat cctgtggagc tccagcgagg cgcccgaggc gggaatcggc
     2281 ctcatggatt acctggccaa cgggcagatc gaccagtggg tccgggaaaa ggtgctgcta
     2341 ccggcgtcca cgcagcaatg cgccataccg gccggcattt tcgccaacgc gggcggcgcc
     2401 atgctgcgcg gcatcgccta cggcaacgag ctgaacctgc tgcacccgcc gcgtcccgcg
     2461 gatgcgaggc tcctggccgc gtggaacccg gaatggtcgc tgcgcgtgcg cgtgaaatcg
     2521 acctcgatga cgatgctggg ccaggaggcg gcgccggcga ggcagcagaa gagggaggaa
     2581 ccgaccaatc ccctcgacat cctcaggaaa gggatcttcg gaaaatagcc cggccctagg
     2641 gtcgaagcca gccccgcttc gaggcgtggt aggcgaggat tccgaagatc accgccacgc
     2701 ccacgtgcac cacggtgaag gccgccgtgg tgagcagcac gaagcggccg acccgctcgt
     2761 cgcccgcctc gcggctggag agcgcgagct ccgcgccggc aaggaagagg atcacgccca
     2821 gcaccggctc gggaaagagc ctgagcagga cgtgcaccga gtcggagaag aacagcgcca
     2881 gcagcgcgag aaggacgccc aacatcaccg tggagccgcc ggtgcgcgcg ccgaacttca
     2941 cgtgccccgc catgccgccg gcgccatggc aaaccggaac gccgcccatg gcgctcgacc
     3001 aggcgtttgc gaggcccgtg gagatcgcga tgccgcgttc ggtgaccggt cgatcgggaa
     3061 acaggcggtt gttctcgctc gtgatggcga gcagcgcatt gccgaaggtg agcggaagct
     3121 gcgggagcgc gagcaggacg gcacccgccc agaggtcgcc ggccgataac gcgggccacg
     3181 cgaatgcggg ccaccggaat gccggcgtta tcgccgaaat ctccgccgca agcgccggct
     3241 ggcgcacgag cgcgatgacc accccggccg cgagcagcag gagcatcgtc ggcagccgcg
     3301 aatgcgcgag aagcgcaaac gcaaggagcg ccgcggcgag ccacgcgctg cccgacatca
     3361 tgcggattcc ttccagcatg aagctgaatc ccaggccgag cacgacgccc agcaatgcgg
     3421 gccgcggaat ccattcggcg agcttcttcg ccagtcccgt tgcgcccagc accacccaga
     3481 tcaatgcggt gacgagcccg gagccgacga cgatggacgg cgtgatcgcc gcgcccgccg
     3541 catgggtcgc cgccgccgcg ccgatcgcct tcatcggctg cacgggcacc ggcgtccggt
     3601 accagaggcc ggtggcgatc atcgccccgc cgaatccgag caggaccccg ccggcatcca
     3661 tcttcacgat ggcgaggtag gcgatcacga acggcacgag ggtgccgagg tccccgaacg
     3721 cgcccgagaa ctccttcagg tcgtatcgat tgcgcgcacc ggccgcagcg ggcgatggat
     3781 gttcgacttt catttgttgc ggatcatagc ggccggcggc acttgccggc taaagttgca
     3841 ggcaccgata ccgaaatgtc ccgaggcccc atgcgcactg cccccagcct gttcctcgcc
     3901 gcttccgtcc tcgccgccgg ctgcggctcg atgtccccgg cagaccacgc ccagcacatg
     3961 ggccacgatg ccgccgtggc agacggacgc caggtcgtcg atttccccga gcccatgcgc
     4021 acgcacacgc tctccaacat gcgcgaccac ctcgcggcgc tgcaggagat ccagcaggcc
     4081 ctcgccgaga
//
LOCUS       DASUEV010000067        13957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_134003, whole genome shotgun sequence.
ACCESSION   DASUEV010000067 DASUEV010000000
VERSION     DASUEV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134003"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFV55_02105"
     CDS             <1..370
                     /locus_tag="VFV55_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943114.1"
                     /translation="EPLGGDTWRVRLAVQNTGYLPSYATKRALERKIVRGVIYEIGLP
                     DGAALVSGKFRVEGSQLEGRASKVTLQAFLPDPLVTADRGQCEWTVRAPSGTTITLSA
                     RHDRAGRAATTLMLGSSHEE"
     gene            complement(630..2312)
                     /locus_tag="VFV55_02110"
     CDS             complement(630..2312)
                     /locus_tag="VFV55_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX4943115.1"
                     /translation="MHPAFDRFHRYDELTAVLRAFEKRNAGLFRVESIGRSHEGRDIW
                     IVVATNRDTGPDADKPGFWVDGNIHAGELTASTACLYLLKTLEEQYGKRDDVTRLLDT
                     RALYICPRVNPDGAEWALADKPRYIRSSTRPYPFDEEAVDGLDVEDVDGDGRILSMRI
                     ADANGGYKAHPADPRLMTPRDPAESGGQYWRVIPEGSIRNFDGVEIRVNRDREGLDLN
                     RNFPAGWRQEYQQLGAGPYPASEPEVRAVVDYIVKHPNLCFGVSFHTHSGVILRPFSS
                     EPDENMPAEDLWLYKLFGKKATELTGYPNISIFHDFKYHPKQVITGGFDWIYEHLGQF
                     FWTVEIWAPVKEAGVESYHMIDWYREHPPEDDLKLIAWSDRELGGEGYVDWYPFDHPQ
                     LGRIELGGWNKLATFRNPPAKYLEREVKRFPDWLVWQALTTPKLEILQATAEPLGGDT
                     WRVRLAVQNTGYLPSYATKRALERKIVRGVIYEIGLPEGAALVAGKHRVEGAQLEGRA
                     SKVTLQAFLPDPLVTADRGQCEWTVRAPSGTTITLSARHDRAGRAAVKLALG"
     gene            complement(2391..3887)
                     /locus_tag="VFV55_02115"
     CDS             complement(2391..3887)
                     /locus_tag="VFV55_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4943116.1"
                     /translation="MDVWAQLLNGFAVALTAQNLMWALVGVTLGTAVGVLPGVGPAVT
                     VALLLPITTKVEASGALIMFAGVYYGAMYGGSTTSILLNTPGESATIVTAMEGNQMAK
                     KGRAGPALATSAIGSFVAGTIATIGLTFLSPVVVELGLRFGPPEYFALMIVAFTTVSS
                     VLGDSTLRGMVSLFLGLAIGLVGIDNQTGQARFSYDIPQLLDGIEVVTIAVGLFAVGE
                     TIYFASKMNQVQEKLEMLTGSIWMKADDWKRSWKPWIRGTLIGFPFGTIPAGGAEIPT
                     FLSYAIEKKLAKHPEEFGHGAIEGVAGPEAANNASSTGTMVPLLTLGLPTSATAAIML
                     SAFQQYGLQPGPLLFDSNAALVWGLIASFYVGNVMLLVLNLPLVGVWVKLLEIPKPQL
                     YAGILVFATMGVYGMRQSPFDVFLLYVVGLLGFVMRRFGFPAAPVIVGMILGPLAEEQ
                     MRRSLAISQGDPMIFLTHPISAGLLALAVAVIVAPWLWRRFGPGTKLA"
     gene            complement(3888..4373)
                     /locus_tag="VFV55_02120"
     CDS             complement(3888..4373)
                     /locus_tag="VFV55_02120"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4943117.1"
                     /translation="MAQSRPKGQIAVSAGVLALGVLVLVGGWDLPAGGGYAQVGPGVV
                     PRVVGVALLLLGAMLLREALTGGYRGVDEEAEVHLPMDWKSFAWVSAGILIYGVLVER
                     AGFLIASTVLFVAVARGFNSRRWLLNAAVGLVIASFIFSVFNYGLGLQLPAGVLRGIL
                     P"
     gene            complement(4408..5373)
                     /locus_tag="VFV55_02125"
     CDS             complement(4408..5373)
                     /locus_tag="VFV55_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX4943118.1"
                     /translation="MARLSRHSLLRAAALAAGISIAGSAFALDSVKMMIPANPGGGWD
                     TTGRELGKALLASGAVKNIQYDNKGGAAGAIGLAQFVSASKGDPNALMMGGMVMVGGT
                     IQNKSAVTLDQVTPVARLTSEYEVIVVPAASPIKDMKDLMAKFKANPGSISWGGGSAG
                     GTDHILAGLLAKEVGVEPGKVNYVPFKGGGEAVAAIIGGHVSAGISGLSEFSQHIQSG
                     KMRALAISSAKTMDKIPSLKEQGFNVELANWRGIFGAPGISKAQQAELVKAVETATKH
                     KSWEDSLKRQEWSNFWLPGDEYGAFVQSEVKRIGEILAGLNLGKK"
     gene            complement(5507..6427)
                     /locus_tag="VFV55_02130"
     CDS             complement(5507..6427)
                     /locus_tag="VFV55_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX4943119.1"
                     /translation="MSLVTVLAALIAQYAYPPPERPPLAALYGRLCLSTSKRLNAGDR
                     NSGILAFGALVAVVIVPLGLVAMLAASIHAVVLAALNAAVLYGSLRFLHTTANIAAIE
                     RSLREGESAAASRRLAQWQGEAPPSDEPGATARLAAEHGLREAHHGTFAILFWFLVLP
                     GPLGAVLYPLTQRAARLWDLGVDTDDREFGWFAGRAFEILDWVPQRVTAFVFAIVGDF
                     EDALFCWRSQAAQWLRPEEGIVLASGAGALRVRLGEPIARGEALIDRPALGTGEMAGE
                     DALASLEGMLWRALVLWLVLFLLVAALLAS"
     gene            6562..7530
                     /locus_tag="VFV55_02135"
     CDS             6562..7530
                     /locus_tag="VFV55_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HEX4943120.1"
                     /translation="MLRRLVAALAALVACTAAFAQDYPTRTITIVVPFSAGGPTDTVT
                     RLVAQSMSKSLGQTVIVENVAGAGGTIGPKRVAAAKPDGYQLVLMHIGISTSVSLYRN
                     LGYNPVTDLEPIGLITDVPMTFIARKDFPPKDMKELIAYVKANKDKVTYANAGVGAAS
                     HLCGMLLMTAIQADLTTVPYKGTGPAMTDLIGGQVDFMCDQTTNTTPQIRGGKVKAYA
                     VTSAARIKTFPDLPTVQESGIKDFQVGIWHGLWAPKGTPKPVIEKLAGALRVALKDPA
                     VITKFADLGTEPVSQDQATPAALAKHLKAEVDKWAPIIKKAGVYAD"
     gene            complement(7799..8383)
                     /locus_tag="VFV55_02140"
     CDS             complement(7799..8383)
                     /locus_tag="VFV55_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4943121.1"
                     /translation="MSAALPVVPPAIPRRGSALWRWIGRTVMRISGWRFEGAFPDTGR
                     LVIAVAPHTSNWDFVHGAAAMFALDLELSFIGKHTLFVWPFAAFFRWMGGIPVNRSAA
                     HGVVGQAVDSFRNTPSRVLAIAPEGTRRKVERFKTGFLHIARGAGVPVLLIGLDYPTR
                     TVRIGPVIEIGADIDAERRRVEAHFATVTGRHPQ"
     gene            complement(8380..9357)
                     /locus_tag="VFV55_02145"
     CDS             complement(8380..9357)
                     /locus_tag="VFV55_02145"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEX4943122.1"
                     /translation="MNDLGGAQAVTWADVESAAQRLEGVAHRTPVMTSRTFDTQTGTR
                     AFFKCENFQRMGAFKFRGAYNALSRLSPEERKRGVVAFSSGNHAQAVALAGRILGIPA
                     TIVMPADAPAVKVAATKGYGAEVVLYDRAAGQDREQFAARISAERGSTVIPPFDHAHV
                     IAGQGTAAKELLEQTGPLDWLFVCCGGGGLLSGCAVAARHLSPGVKVVGVEPAAGDDA
                     ARSFRTKSLQRVDDPQTIADGARTQSLGRLTFPLVLANVHDFLTVTDGELVEAMRFLW
                     ERMKLVVEPTGALATAGVLSGRLDTRGARVGIILSGGNVDLRSAAKLFA"
     gene            complement(9366..9572)
                     /locus_tag="VFV55_02150"
     CDS             complement(9366..9572)
                     /locus_tag="VFV55_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX4943123.1"
                     /translation="MNPETFNMSIRKFLKSVGVQSQREIEGAVERAIASGKLKGGETL
                     PAKMTLAIDGLALSVTIDGRIALE"
     gene            complement(9585..9794)
                     /locus_tag="VFV55_02155"
     CDS             complement(9585..9794)
                     /locus_tag="VFV55_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943124.1"
                     /translation="MLGKEIDILMAERARLLKVAGAAAQFVALMESRALPESVAMEAE
                     LLAETVNALPEDSLRDALASIKRPK"
     gene            complement(9909..10952)
                     /locus_tag="VFV55_02160"
     CDS             complement(9909..10952)
                     /locus_tag="VFV55_02160"
                     /inference="COORDINATES: protein motif:HMM:NF039736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX4943125.1"
                     /translation="MAQDFWASSGFRLLGRGPEGWLVPGDDYLRHFLARAELLPPPEA
                     GAGERHLHARLLDKPRLAVGDADLAAVEDEDARDNWVQFLLFRDRLLAAGSLEACYVE
                     QFRRSQGGGPDRQVGVEFAPPFIDALAQAIVRGLLEGTADPWLARAGELFFRRQRVSV
                     EGGMVLAADASTIEAYAETGGFGNVGRLLRAQQTPLAAVKLDVLNAENAPFYFLRDEF
                     FSFLLDLTPGREGARALAAVLERWVAHLAGVRVAIEPLARIEDERWRWHAGLDVESTA
                     ILNALFRGESVSDEARSRLVSLFRLTFADAGDALPEAAGRPVYLGLAFRDDHTLKLKP
                     QNLLVNLPLARMS"
     gene            complement(10954..11430)
                     /locus_tag="VFV55_02165"
     CDS             complement(10954..11430)
                     /locus_tag="VFV55_02165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4943126.1"
                     /translation="MPVTGEPRPAFEPADLAYERRTRESFDRQGAMAHLGARLGRVEP
                     GFVEIELAFRPELSQQHGFFHAGIVATIADSAAGYAAYTLFPAQAGVLTVEFKVNLMA
                     PADGERIVAAGHVIRSGRTITVAQFDVHVEKAGRRTHCATGLQTIMRIVGRTGVQG"
     gene            complement(11438..12622)
                     /locus_tag="VFV55_02170"
     CDS             complement(11438..12622)
                     /locus_tag="VFV55_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX4943127.1"
                     /translation="MPRAVVKKLRPVPEFPLDTHPEVSLDFGRVKQYLARHAYRKPVL
                     LVDLDIVRAKTRRFRAALPRVRPHFAVKANPDPRVLKVLIQEGAGFEVASIAELDLLL
                     TLGVPAAEVFYSNPMKSRAYLEHASAKGVEWYVVDSVEEIRKVQSVKADAKLYLRIDV
                     PNIGSDWPLAGKFGAHMTDVPELIAAAVDCKADLAGITFHVGSQCLNPENWRVGIERA
                     RKVFAAMRQAGLSPRLLNLGGGYPVRHVKPIPSIETIADVINRELRHFGPEIRVIAEP
                     GRYLVSDAGYFICRIVGTATRNGQRWMYWDAGVFGGVIETTEGLRYNLYTDRDGEPVS
                     WNVAGPTCDSVDVVMRDELLPADLQENDFIFIKNAGAYTTAYASNFNGFPLPDVAVLG
                     AV"
     gene            complement(12702..13268)
                     /gene="dcd"
                     /locus_tag="VFV55_02175"
     CDS             complement(12702..13268)
                     /gene="dcd"
                     /locus_tag="VFV55_02175"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX4943128.1"
                     /translation="MSIKSDKWIRRMAASHAMIEPFEPGQVREVDGRRIVSYGTSSYG
                     YDIRCSTEFKIFTNINATIVDPKNFDEKSFVNFSGDVCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGFVTLEFSNTTPLPAKIYAGEGCAQVIFF
                     ESDEVCETSYRDRGGKYQGQQGVTLPKM"
     gene            complement(13292..>13957)
                     /locus_tag="VFV55_02180"
     CDS             complement(13292..>13957)
                     /locus_tag="VFV55_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX4943129.1"
                     /translation="QPTMLGISGSPHTEDGKVLEPMVGHGIEAMSIGFLIDNDTPMVW
                     RGPMVTQALEQLVRDTHWGNLDYLVVDLPPGTGDTQLTLAQKVPVTGAVIVTTPQDIA
                     LIDARKGLKMFEKVGVPILGIVENMAIHVCSKCGHAEHIFGHGGGERMCKDFDVEFLG
                     GLPLDIGIREQTDSGKPTVVADPDGKIAAIYRDIARKVAARIAKQAEDRTAIFPKIVV
                     QNT"
BASE COUNT         2319 a         4731 c         4746 g         2161 t
ORIGIN      
        1 cgagcccctc ggcggcgaca cgtggcgcgt gcggctcgcg gtgcagaaca ccggctacct
       61 gccgagctac gccaccaagc gtgcgctgga gcgaaagatc gtgcgcggcg tgatctacga
      121 gatcgggctg ccggatgggg ccgcgctcgt gtcgggcaaa ttccgcgtgg agggcagcca
      181 gctcgagggc cgcgccagca aggtgacgtt gcaggcgttc ctgccggacc cgctggtcac
      241 ggccgaccgc gggcaatgcg agtggaccgt gcgggccccg agcgggacga cgatcacgct
      301 ctcggcaagg cacgaccgcg ccggccgggc ggcgacgacg ctgatgcttg gctcgtcgca
      361 cgaggagtag gcagcgtggg acgacgaagt tcaggaccgt gaaaaccggc gaagaagtcg
      421 aatcaggacg acgaagttcg ggggcctgtt ggttgcgttt ttcttcgtct cgggccagat
      481 caacttgcca tcagccgcga cgccggacgc gattcgacat aggttcgatt cgcgacattc
      541 gacgcaattc aacctggcca aagacgaaga aaaacacaac caacaggccc ccgaacttcg
      601 tccttgctag tcacgttgtc ctcgtgcgct cagcccagcg ccagcttcac tgcggcccgg
      661 ccggcgcggt cgtgccgtgc ggagagcgtg atcgtcgtcc cgctcggggc ccgcactgtc
      721 cactcgcact gcccgcggtc ggccgtgacc agcgggtccg gcaggaacgc ctgcagcgtc
      781 accttgctgg cgcggccctc gagctgggcc ccctcgacgc ggtgcttgcc ggcgacgagc
      841 gcggcccctt ccggcagccc gatctcgtag atcacgccgc gcacgatctt tcgctccagc
      901 gcacgcttgg tggcatagct cggcaggtag ccggtgttct gcaccgcaag ccgcacgcgc
      961 cacgtgtcgc cgccgagggg ctcggccgtc gcctggagaa tctcgagctt cggcgtcgtg
     1021 agcgcctgcc acacgagcca gtcgggaaag cgcttcactt cgcgttcgag gtactttgcc
     1081 ggcggattgc ggaaggtggc gagcttgttc cagccgccca gctcgatgcg gccgagctgc
     1141 gggtggtcga acgggtacca gtcgacgtag ccctcgccgc cgagttcccg gtcgctccag
     1201 gcgatgagct tcaggtcgtc ctcgggcggg tgctcgcggt accagtcgat catgtggtag
     1261 ctctcgacac cggcctcctt gaccggcgcc cagatctcca ccgtccagaa gaactggccg
     1321 aggtgctcgt agatccagtc gaagccgccg gtgatgacct gcttcgggtg gtacttgaag
     1381 tcgtggaaga tcgagatgtt cgggtagccg gtgagctcgg tcgccttctt gccgaagagc
     1441 ttgtacagcc acaggtcctc ggccggcatg ttctcgtcgg gctcgctgga gaacgggcgc
     1501 aggatcacgc cgctgtgggt gtggaagctc acgccgaagc acaggttcgg gtgcttcacg
     1561 atgtaatcga cgacggcccg cacctcgggc tccgaagccg gatagggccc ggcacccagt
     1621 tgctggtatt cctggcgcca gcccgccgga aaattgcggt tgaggtcgag cccttcgcgg
     1681 tcgcggttca cgcggatctc gacgccgtcg aagttgcgga tgctgccctc gggtatcacg
     1741 cgccagtact gcccgccgga ttcggcgggg tcgcgcggcg tcatcaggcg cgggtccgcc
     1801 gggtgcgcct tgtagccgcc gttggcgtcg gcgatgcgca tcgagaggat gcgtccgtcg
     1861 ccgtccacgt cctcgacgtc caggccgtcc accgcctcct cgtcgaaggg atagggccgc
     1921 gtcgaggagc ggatgtatct cggcttgtcg gccagcgccc attcggcccc gtcgggattg
     1981 acgcgcgggc agatgtagag ggcgcgcgtg tcgaggaggc gcgtcacgtc gtcgcgcttg
     2041 ccgtattgct cctcgagggt cttgagaagg tacaggcacg ccgtggaggc cgtgagctcg
     2101 ccggcgtgga tgttgccatc gacccagaag ccgggcttgt ccgcatccgg ccccgtgtcg
     2161 cggttggtgg cgaccacgat ccagatgtcg cgcccttcgt ggctcctgcc gatgctctcg
     2221 acacggaaga ggccggcgtt gcgcttctcg aaagcgcgca ggaccgcggt gagttcgtcg
     2281 taacggtgga agcggtcgaa agcggggtgc atggcactcc ttgagcggtg tcagattcct
     2341 ctaggggcgg gcaggtccgg aatccgacac cgcgaaagga agtccctctc ctacgcgagt
     2401 ttcgttcccg gcccgaaccg gcgccacagc cacggcgcga cgatgacggc cacggccagg
     2461 gcgagcaatc cggcggagat cggatgggtc aggaagatca tcggatcgcc ctggctgatg
     2521 gcgagcgagc ggcgcatctg ctcttccgcc agcggcccga ggatcatgcc cacgatcacc
     2581 ggtgccgccg ggaagccgaa gcggcgcatg acgaagccca gcaggccgac gacatagagc
     2641 aggaacacgt cgaacggcga ctggcgcatc ccgtacacgc ccatcgtggc gaagacgaga
     2701 atgcccgcgt aaagctgcgg cttgggaatc tcgaggagct tcacccagac cccgacgaga
     2761 ggcaggttga gcacgagcag catcacgttg cccacgtaga agctcgcgat caggccccac
     2821 accagcgccg cattcgaatc gaaaagcagc ggcccgggct gcaggccgta ctgctggaac
     2881 gccgacagca tgatcgccgc cgtggccgag gtgggaagcc ccagcgtgag gagcggcacc
     2941 atcgtccccg tggacgaggc gttgttggcg gcctcgggtc ccgccacccc ctcgatcgcc
     3001 ccgtggccga actcctcggg gtgcttcgcc agcttcttct cgatggcgta ggagaggaag
     3061 gtcggaatct ccgcgccgcc cgccggaatg gttccgaacg gaaagccgat gagcgttcca
     3121 cggatccacg gcttccagga acgcttccag tcgtcggcct tcatccagat ggacccggtg
     3181 agcatctcga gcttctcctg cacctggttc atcttcgagg cgaaatagat cgtctcgccc
     3241 acggcgaaga ggcccacggc gatggtcacg acctcgatgc catcgaggag ctgcgggatg
     3301 tcgtaggaga agcgggcctg gcccgtctgg ttgtcgatgc cgacgagccc gatcgcgagc
     3361 ccgaggaaca ggctcaccat gccgcgcagg gtcgagtccc ccagcaccga cgacaccgtg
     3421 gtgaaggcaa cgatcatcag cgcgaagtac tccggcgggc cgaacctgag cccgagttcc
     3481 acgaccacgg gcgacaggaa ggtgagcccg atggtggcga tcgttcccgc cacgaaggac
     3541 ccgatggcgc tcgtggccag cgcgggtccc gcccggccct tcttggccat ctggttgcct
     3601 tccatcgcgg tgacgatggt cgcgctctcg cccggcgtgt tgagcaggat cgacgtggtg
     3661 gagccgccgt acatcgcgcc gtagtagacg ccggcgaaca tgatgagcgc cccgctcgcc
     3721 tccaccttgg tggtgatggg aagcagcagc gccaccgtga ccgcaggccc cacgccggga
     3781 aggacgccga cggcggtacc gagcgtcacg cccaccagcg cccacatcag gttctgcgcg
     3841 gtaagcgcga cggcgaaacc gttgagcagc tgcgcccaga cgtccatcta gggaaggatc
     3901 ccgcgcagca cgcccgccgg cagttgcagg ccgaggccgt agttgaacac cgagaagatg
     3961 aatgacgcga tgaccaggcc cactgccgcg ttgagcagcc agcggcggct gttgaacccg
     4021 cgcgccaccg cgacgaacag gaccgtcgag gcgatgagga agccggcacg ctcgacgagc
     4081 acgccgtaga tgaggattcc ggcggagacc catgcgaacg acttccagtc catcgggagg
     4141 tggacctcgg cttcctcgtc cacgccgcgg tagccgcccg tgagcgcctc gcgaagcagc
     4201 atggcgccaa gcaacaggag cgcgacgccg acaacgcgcg gcacgacccc cggtcctacc
     4261 tgcgcatagc cgccacccgc gggcaggtcc cagccgccca cgagcaccaa cacgcccagc
     4321 gcaagcacgc cagcggaaac cgcgatctgc cccttcgggc gggattgggc catcgggaag
     4381 agacgcccga ttcctcgggc gtccgcatca cttcttgccg aggttcagcc cggcgaggat
     4441 ctcgccaatg cgcttcactt ccgactgcac gaaggcgccg tactcgtcgc caggcagcca
     4501 gaaattgctc cattcctgcc gcttgaggga gtcttcccag gacttgtgct tcgtggccgt
     4561 ctcgacagcc ttcacgagtt ccgcctgctg cgccttgctg atgccgggcg cgccgaagat
     4621 gccgcgccag ttggcgagct cgacgttgaa gccctgctcc ttgaggctcg ggatcttgtc
     4681 catcgtcttg gccgaggaga tcgccagggc gcgcatcttg cccgactgga tgtgctgcga
     4741 gaattccgac agccccgaaa tgcctgccga cacgtggccg ccgatgatcg ccgcgaccgc
     4801 ctccccgccg cccttgaacg gcacgtagtt caccttgccg ggctcgacgc ccacttcctt
     4861 ggcgagcagg cccgcgagga tatggtcggt tccgccggcg ctgccgccgc cccacgagat
     4921 gctgcccggg ttcgccttga acttcgccat caggtccttc atgtccttga tgggggacgc
     4981 ggcaggcacg acgatgacct cgtattccga ggtgaggcgc gcgaccggcg tgacctggtc
     5041 cagggtgacg gccgacttgt tctggatcgt gccgccgacc atgaccatgc cgcccatcat
     5101 gagggcattc gggtcgccct tcgaggcgct cacgaactgc gccaggccga tggcgcccgc
     5161 ggcgccgccc ttgttgtcgt actggatgtt cttcaccgcg cccgaggcga gcaatgcctt
     5221 gccgagttcg cggcccgtgg tgtcccagcc gccgccgggg tttgcgggga tcatcatctt
     5281 cacgctgtcg agcgcaaagg cggaaccggc gatcgaaatg ccggcggcca gcgccgcggc
     5341 gcgcagcagc gagtgtcggg aaagacgtgc catcgttggt ttctcctcgt ctggaatcgg
     5401 tatgtccctt cgggggggcc tcgacggccc ttcctcgagg atcgccgcga gtgtaacaaa
     5461 tgcccccctc cgccgcacgg cggggcgctg gacttgccgc aacggctcag gatgcgagga
     5521 gggcggcgac aaggaggaaa aggaccagcc agagcacgag cgcgcgccac agcatgccct
     5581 cgaggctggc gagggcatcc tcgccggcca tctctcccgt gcccagcgcc ggccggtcga
     5641 tgagcgcctc gccgcgcgcg atcggctctc ccaggcgaac gcgcagggcg cccgcgccgc
     5701 tcgcgaggac aatgccctct tcggggcgca gccattgcgc ggcctgcgat cgccagcaga
     5761 aaagcgcgtc ctcgaaatcg ccgacgatcg cgaacacgaa ggcggtcacg cgctgcggaa
     5821 cccagtcgag gatctcgaag gcgcgtcccg cgaaccagcc gaattcgcgg tcgtcggtat
     5881 ccacgcccag gtcccagagg cgcgcggcgc gttgcgtgag cggatacagc accgcgccca
     5941 gcgggcccgg aaggacaagg aaccagaaga ggatcgcgaa ggttccgtgg tgcgcttccc
     6001 gaaggccgtg ctcggcagcc aggcgcgccg tggccccggg ctcgtcggac ggaggagcct
     6061 cgccctgcca ctgggccagc cgccgcgacg ccgcggcgct ttcgccttcg cggaggctgc
     6121 gctcgatcgc cgcgatgttc gcggtggtat gcaggaagcg caggcttccg tagaggaccg
     6181 ccgcgttcag ggccgcgaga accaccgcgt ggatcgaggc cgccagcatc gcgacgagcc
     6241 cgagcggcac gatcaccacg gcgaccagtg ccccgaacgc gaggatgccg gaattgcggt
     6301 cgcccgcatt gagccgcttg gacgtcgaca ggcaaaggcg gccgtacagg gcggccagcg
     6361 gcggcctttc gggcggggga taggcgtact gcgcaatgag ggcggcaagg acggtgacga
     6421 ggctcaaggc gggctcctgt atcgcctcga ttataggtgc cgggacaccc cgccgtgagc
     6481 caaaaacggg ataatccagc ctcgcggccg ccgcagcgcg gcccgatggg aacccgaata
     6541 atcaagaccg aggagagatc gatgttgcgc agactcgttg ccgccctggc agcccttgtc
     6601 gcctgtacgg cggctttcgc ccaggactac cccacccgaa ccatcacgat cgtcgtgccg
     6661 ttctcggccg gcgggccgac cgacaccgtg acgcgcctcg tcgcgcaatc gatgtcgaag
     6721 tcgctcggcc agacggtgat cgtcgagaac gtggccggcg ccggcggcac gatcggcccg
     6781 aagcgcgtcg cggccgccaa gcccgacggc taccaactcg tgctcatgca catcggcatc
     6841 tcgaccagcg tgtccctcta ccgcaacctc ggctacaacc cggtcaccga cctcgagccc
     6901 atcggcctca tcaccgacgt gccgatgacg ttcatcgcgc gcaaggactt cccgccgaag
     6961 gacatgaagg aactcatcgc ctacgtcaag gcaaacaagg acaaggtcac ctacgccaac
     7021 gccggcgtgg gcgctgcctc gcacctttgc ggcatgcttc tcatgaccgc gatccaggcc
     7081 gacctcacca ccgtccccta caagggcacc gggccggcca tgaccgacct catcggcggc
     7141 caggtggact tcatgtgtga ccagacaacg aacacgacgc cgcagatcag gggcgggaag
     7201 gtcaaggcgt acgccgtgac ctccgcggcc cgcatcaaga cgtttcccga cctgccgacg
     7261 gtccaggaat ccggcatcaa ggacttccag gtcggcatct ggcacggatt gtgggctccc
     7321 aagggcacgc cgaaaccggt catcgaaaag ctcgccggcg ccctgcgggt cgccctcaag
     7381 gacccggccg tgatcaccaa gttcgccgac cttggcaccg aacccgtttc gcaggatcag
     7441 gctacgcccg ctgcgctggc caagcatctc aaggcggaag tcgacaagtg ggccccgatc
     7501 atcaagaagg cgggcgtcta cgccgactga tcctccgctt gcgcgcggcc gaaaaaaacc
     7561 cgccggggta accggcgggt caaaccgctc tcggaggaga aactgccggc atgcccggaa
     7621 gcaggccgac aggacaaaga tctgcaggga ttgtgccaaa cggggcgggc ggataattcc
     7681 gtgcgccgtc agggcgttac gaggtgaccc gcgattccgg gcgaagcgcg aagcggcaca
     7741 ttttggtgcg ccgcggcacc agcttggacc tgagcatgca atccccggcg cccggcgcct
     7801 actgcggatg ccggccggtt acggtcgcga aatgcgcctc gacgcgccgg cgctcggcgt
     7861 cgatgtccgc gccgatctcg atcaccggcc cgatgcgcac ggtgcgcgtc gggtagtcga
     7921 gcccgatcag gaggacgggg acccccgcac cccgcgcgat gtgcaggaac cccgtcttga
     7981 agcgctcgac tttccggcgc gtgccctcgg gcgcaatcgc cagcacccgc gacggggtgt
     8041 tgcgaaacga gtccaccgcc tgccccacca ccccatgggc cgcggagcgg ttgacgggaa
     8101 tgccgcccat ccaccggaag aaggccgcga agggccacac gaagagcgtg tgcttgccga
     8161 tgaaggagag ctcgaggtcc agcgcgaaca tcgcggccgc gccatgcacg aagtcccagt
     8221 tggaagtgtg cggcgccacc gcgatcacga gccttccggt atcgggaaag gccccttcga
     8281 atcgccagcc gctgatgcgc atcaccgtgc gtccgatcca tcgccacagc gcggagccgc
     8341 gccgcggaat ggccggcggc accacgggca gcgcggcgct cacgcgaaga gcttggcggc
     8401 ggagcgcagg tcgacgttgc cgccggagag gatgatgccg acgcgtgcgc cgcgcgtgtc
     8461 gagccggccc gagagcacgc cggcggtcgc cagcgccccc gtgggctcga ccacgagctt
     8521 catgcgctcc cacaggaagc gcatggcctc caccagttcc ccgtcggtga cggtgaggaa
     8581 gtcgtggaca ttggccagca cgagcgggaa cgtgagccgg cccagcgatt gcgtgcgggc
     8641 gccgtccgcg atggtctggg gatcgtcgac gcgctggagc gacttggtgc gaaaggagcg
     8701 cgcggcatcg tcgcccgccg ccggttccac cccgacgacc ttgacgccgg gtgacaggtg
     8761 gcgcgcagcg acggcgcaac ccgacaggag cccgcctccg ccacagcaca cgaagagcca
     8821 gtcgagcggc ccggtttgct ccagcaattc cttggccgcc gtgccctgcc ccgcgatcac
     8881 gtgcgcatgg tcgaaggggg gaatgaccgt ggacccgcgc tcggccgaaa tgcgggcggc
     8941 gaattgctcg cggtcctggc cggcggcgcg gtcgtacagg accacctcgg cgccgtagcc
     9001 cttggtggcc gccactttca ccgcgggcgc atcggccggc atcacgatcg tggccggaat
     9061 gccgaggatc cttccggcga gcgccacggc ctgggcgtga tttcccgacg aaaacgccac
     9121 gacgccgcgc ttgcgttcct cgggcgacag gcgcgaaagc gcgttatagg cgccgcggaa
     9181 cttgaacgcc cccatgcgct ggaagttctc gcacttgaag aacgcccggg tgccggtctg
     9241 cgtgtcgaaa gtccgggagg tcatcaccgg cgtgcggtgc gcaacgccct ccaggcgctg
     9301 cgcggccgac tcgacgtcgg cccaggtgac ggcctgagcg ccgccgaggt cgttcacggc
     9361 gccgcctatt cgagcgcgat gcgcccgtcg atggtcacgc tgagcgcgag gccgtcgatg
     9421 gcgagcgtca tcttcgcggg cagcgtctcg ccccccttga gctttccgga ggcgatcgcc
     9481 ctttccaccg cgccctcgat ctcgcgctgg gactgcacgc caacgctctt caggaacttg
     9541 cggatgctca tgttgaaggt ctccggattc atcgctttcc tccctcattt gggtcgtttg
     9601 atcgaggcga gcgcgtcgcg aagcgagtcc tcgggaagcg cgttcaccgt ctcggcgagg
     9661 agctcggctt ccatcgcgac cgattcggga agcgcgcggc tttccatgag cgccacgaac
     9721 tgcgccgcgg cgcccgccac cttgagaagc cgagcgcgtt cggccatcag gatgtcgatt
     9781 tccttgccca gcatgaacgc gcttgtgttt tcgctccctg ccgccatgac acgctccgcg
     9841 aatccgatcg aggccctcat cttacctgcg atgggacgtc agctcatccg cttgcccgcc
     9901 atgaggcgtc agctcatccg cgcgagcggc aggttcacga ggaggttctg cggcttcagc
     9961 ttgagcgtgt ggtcgtcgcg gaacgcgagg ccgaggtaga cggggcggcc ggcggcctcc
    10021 ggcagcgcgt cgcccgcgtc ggcgaaggtg agccggaaga gcgacaccag gcgcgagcgc
    10081 gcctcgtcgg aaacggactc gccgcgaaag agcgcattga ggatcgcggt cgactccacg
    10141 tcgagcccgg cgtgccagcg ccagcgctcg tcctcgatgc gcgccagcgg ctcgatggcg
    10201 acgcgcacgc cggcgagatg cgcgacccag cgctcgagga cggccgccag cgcccgcgcg
    10261 ccctcgcgcc ccggcgtgag gtcgaggaga aacgagaaga actcgtcgcg caggaagtag
    10321 aacggggcgt tctcggcatt gagcacgtcc agcttcaccg cggccagggg cgtctgctgc
    10381 gcccggagaa gccggccgac attgccgaat ccgccggtct ccgcgtaggc ctcgatggtg
    10441 gaggcgtcgg ccgccaggac catcccgcct tcgacgctca cgcgttgccg gcggaagaag
    10501 agttcgccgg cgcgggcgag ccaggggtcc gcggttccct cgagaaggcc gcggacgatg
    10561 gcctgcgcca gcgcgtcgat gaacggtggc gcgaactcga cgcccacctg ccggtccggt
    10621 cccccgccct gcgagcgacg gaactgttcc acatagcacg cctcgagcga cccggccgcc
    10681 agcagccggt cgcgaaagag caggaactgc acccagttgt cgcgcgcgtc ctcgtcctcg
    10741 accgccgcga ggtccgcgtc accgacggca aggcgcggct tgtcgagaag cctggcgtgc
    10801 aggtggcgct cgcccgcgcc cgcttcggga ggcggcagca gttccgcgcg ggccaggaag
    10861 tgcctgaggt aatcgtcgcc cggcacgagc cagccctccg gcccgcgccc gagcagccgg
    10921 aagccgctcg acgcccagaa gtcctgcgcc aagctagccc tggacgccgg tgcggccgac
    10981 gatgcgcatg atcgtctgaa gccccgtcgc gcaatgcgtc cggcgcccgg ccttctccac
    11041 gtgcacgtcg aactgcgcga cggtgatcgt tcgtcccgag cggatgacgt gccctgccgc
    11101 cacgatgcgc tcgccatcgg cgggcgccat caggttcacc ttgaactcga cggtgagcac
    11161 gccggcttgc gccgggaaaa gggtgtaggc ggcgtagccc gcggcggaat cggcgatcgt
    11221 cgccacgatc ccggcatgga agaagccgtg ctgctgggaa agctcggggc ggaatgcgag
    11281 ctcgatctcg acgaagcccg gctccacgcg gccgagcctg gcgcccaggt gcgccatcgc
    11341 tccctgccgg tcgaaactct ccctcgttcg ccgctcgtag gccaggtcgg cgggctcgaa
    11401 cgcgggtctc ggctctccgg tcaccggcac gcgcgcctca caccgcgccg agcacggcca
    11461 cgtcgggcag ggggaagccg ttgaagttcg acgcgtacgc cgtggtgtag gcgccggcgt
    11521 tcttgatgaa gatgaaatcg ttttcctgca ggtcggcggg cagcagctcg tcgcgcatca
    11581 ccacgtccac ggaatcgcag gtcggtcccg ccacgttcca cgacaccggc tcgccgtcgc
    11641 ggtcggtgta caggttgtag cgcagtcctt cggtggtctc gatcacgccg ccgaagacgc
    11701 ccgcgtccca gtacatccag cgctggccat tgcgggtggc ggtgccgacg atgcggcaga
    11761 tgaaatagcc ggcatcggaa acgaggtagc gccccggctc ggcgatcacc cggatttccg
    11821 gcccgaagtg cctgagctcc cggttgatca cgtcggcgat cgtctcgatg gacgggatcg
    11881 gcttcacgtg ccgcacgggg tagccgccac ccaggttcag cagccgcggg ctgagccccg
    11941 cctggcgcat ggccgcgaag accttgcgcg cgcgctcgat gcccacgcgc cagttctccg
    12001 ggttgaggca ctgggagccg acgtggaagg tgataccggc gaggtccgcc ttgcagtcga
    12061 ccgcggccgc aatcagctcg ggaacgtcgg tcatgtgcgc cccgaacttg ccggcgagcg
    12121 gccagtcgct gccgatgttg ggcacgtcga tgcgcaggta gagcttcgcg tcggccttga
    12181 ccgactgcac cttccggatc tcctcgaccg agtcgacgac gtaccactcg acgcccttgg
    12241 cgctggcatg ctcgaggtag gcgcgcgact tcatcgggtt cgagtagaac acctcggcgg
    12301 cgggcacgcc cagggtgagg agaaggtcca gctccgctat cgaggcgacc tcgaagcccg
    12361 cgccctcctg gatcagcacc ttgaggacgc gcgggtcggg gttcgccttg acggcgaagt
    12421 gcggccgcac gcgcggaagg gccgcgcgaa accgccgggt cttggcgcgc acgatgtcga
    12481 gatcgacgag gagaaccggc ttccggtacg catgccgggc gagatactgc ttgacgcggc
    12541 cgaagtcgag gctgacttcg gggtgggtat cgaggggaaa ctccggcacg gggcggagct
    12601 tcttgactac ggctcggggc atcgacgtct ttcgtgacgg gtcatcgggg cgcggggtgc
    12661 gaaaaattgg gcgcagtata gggcaattcg cccgtggcgc gtcacatctt gggcagcgtg
    12721 acgccctgtt gcccctggta cttgccgccg cggtcgcggt aggaggtctc gcagacctcg
    12781 tcggactcga agaagatcac ctgcgcgcag ccttcgccgg cgtagatctt ggccggcagc
    12841 ggcgtggtgt tggagaactc cagggtcacg aatccttccc actccggctc gaaaggcgtc
    12901 acgttgacga tgatgccgca gcgtgcgtag gtggacttcc cgaggcagac ggtgaggacg
    12961 ttccggggaa tgcggaaata ctcgacggtg cgcgccagcg cgaaggagtt gggcgggatg
    13021 atgcagacgt cgcccgagaa gttgacgaac gacttctcgt cgaaattctt cgggtcgacg
    13081 atggtcgcgt tgatgttggt gaagatcttg aattcggtgg agcagcggat gtcgtagccg
    13141 tagctcgagg tgccgtagga cacgatccgc cggccgtcga cctcgcgcac ctggccgggc
    13201 tcgaagggct cgatcatggc gtggctcgcc gccatgcggc gaatccactt gtcgctcttg
    13261 atgctcatgg ggtgccgcgc cgccttccgg ttcaggtgtt ctggacgacg atcttcggaa
    13321 agatcgcggt gcggtcctcc gcctgcttcg cgatgcgcgc ggccaccttg cgcgcgatat
    13381 cgcggtagat cgcggcgatc ttcccgtccg ggtcggcgac cacggtgggc ttgccgctgt
    13441 cggtctgctc gcgaatgccg atgtcgagcg ggagcccgcc gaggaactcc acgtcgaagt
    13501 ccttgcacat gcgctcgccg ccgccgtggc cgaagatgtg ctcggcatgg ccgcacttgg
    13561 agcacacgtg gatcgccatg ttctcgacga tccccaggat gggcacgccg accttctcga
    13621 acatcttgag gcccttccgc gcgtcgatga gggcgatgtc ctggggggtg gtgacgatca
    13681 ccgccccggt cacaggcacc ttctgcgcaa gcgtcagttg cgtgtccccg gtgcccggcg
    13741 gcaggtcgac cacgaggtag tccaggttgc cccagtgagt gtcgcgcacc aactgttcca
    13801 gtgcctgggt caccatcggc ccgcgccaca ccatcggcgt gtcattgtcg atcagaaagc
    13861 cgatcgacat cgcctcgatg ccgtgcccca ccatgggctc gagtaccttg ccatcctccg
    13921 tatgcgggct gccggaaatt cccagcatcg tcggctg
//
LOCUS       DASUEV010000068         7001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_707032, whole genome shotgun sequence.
ACCESSION   DASUEV010000068 DASUEV010000000
VERSION     DASUEV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707032"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VFV55_02185"
     CDS             <1..1915
                     /locus_tag="VFV55_02185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX4943130.1"
                     /translation="RHFAAGGSFERALEGWRVRPQQLEFAEAVLAAIEASAVLIAEAG
                     TGTGKTFAYLAPALIAGGRVIVSTGTKTLQDQLFHRDLPLVRDTLGVPARLALLKGRS
                     NYVCLHHVEESSAEGTFASREEARDIHAIVRFAGRSATGDKAECLEVSELSGAWARAT
                     STRENCLGSKCRHYEDCFVMKARKAANDADLVVVNHHLFFADVVLRDEGVKDLLPSAN
                     TIIFDEAHHLPDLARLFFGQAVSTAQVIELARDARSAQILHARESPAIGEAAQAADRA
                     ARDVRIALGTGTGRFGAAQFEANADLAKALDALDERLGALGGLLAAQAERADELRSAH
                     TRTVLLRDAIAGWRGEAGEGAVRWVEAYAQSAVLNRTPLDVGPIFAQQLAGEKRAWIF
                     TSATLSVAGDFRHYQSELGLHEATTRHWESPYDFANQALLYVPEGLPEPNSPGHTEAV
                     IDAAFPVIVAAKGRAFLLFTSLRAMDVGHARLKELLASAGLDWPVYLQGTAGKNELLE
                     RFRDTANAILVGSQSFWEGVDVKGEALSLVVIDKLPFNPPDDPVLAARIDQINRAGGN
                     AFMEYQVPRAVIALKQGAGRLIRDETDRGVLMICDPRLVSKPYGKRIWRALPPFRRSR
                     SRDDAVGFFTLSG"
     gene            complement(1916..2509)
                     /locus_tag="VFV55_02190"
     CDS             complement(1916..2509)
                     /locus_tag="VFV55_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943131.1"
                     /translation="MNPMRIALIACLAAAGLAGISEAPAAANSRSSGASSSGPRFSGA
                     APAHRGGFGQSHSGNHRPGSGARWNGGNWDHRSHSHWGWGLGLAFGVPWALGWYDPWY
                     WGPAYYPPYYGYGPVYRSYGYACEQDEDCWRERQSRGEPAPATTEVAPAAPGEEGGPT
                     QRPLHLNYCDSAKAWYPHVRTCPGGWRLVRPDYNPAP"
     gene            complement(2580..2738)
                     /locus_tag="VFV55_02195"
     CDS             complement(2580..2738)
                     /locus_tag="VFV55_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943132.1"
                     /translation="MTVCPIAIVVGCKKCPAFSICPVKTTLGDQPKPGEGAPQKAAAA
                     PARKKKSR"
     gene            complement(2786..4093)
                     /locus_tag="VFV55_02200"
     CDS             complement(2786..4093)
                     /locus_tag="VFV55_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4943133.1"
                     /translation="MKWWAGSFFWRTLAVLVIALVASQAASFWLFRQQVQQPRQAMRI
                     GQFVSHLKTIHAALNTLPPAAEREFFEQLAEKEGISVVPARGNEPGRLAADMPGMHMF
                     RERIKGLFGDDTEVFVRPGVPGVFWVRLHAGGRDFWVAFPRNRVDRDNTDALLYWMLA
                     GVAIAILATALIAWRLNRPLTRLARAADDLGKGGDPPPVPETGPSEVRAVARAFNLMK
                     ESLKNEQRERTTFLAGISHDLRTPLARLRLDVEMLEGRVEAGTQNGMVADIEDMNAII
                     DQFIDFARSEATETYAPVDLSSLARACAERAARAGAQVKCELAETPVLMLRTLAVQRL
                     VDNLIQNAARHGGGEIIVRTTATGGEVTLAVLDRGPGIPAQDIERLKEPFTRRDEARS
                     GRSGAGLGLAIVARIAKAHGARFDLAPREGGGLAAAVAFPLAP"
     gene            complement(4090..4821)
                     /gene="ompR"
                     /locus_tag="VFV55_02205"
     CDS             complement(4090..4821)
                     /gene="ompR"
                     /locus_tag="VFV55_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX4943134.1"
                     /translation="MPTLPRHRILVVDDDLRLRDLLKRYLTEQGFGVETVPDGAAMDH
                     NLKRTRYDLVVLDLMLPGEDGLAICRRLRAAGNELPVIMLTAKGDDVDRIVGLEMGAD
                     DYLAKPFNPRELVARINAVMRRQAPPPAPGAPAREDTVVTFGPFALNLGTRALTKDGA
                     PLALTTGEFSVLKVFAENPREPLSRDKLMDLARGREHESFDRSIDVQVSRLRKLLGED
                     PQSPRYIQTVWGFGYVFIPDGTPQK"
     gene            5017..5736
                     /locus_tag="VFV55_02210"
     CDS             5017..5736
                     /locus_tag="VFV55_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943135.1"
                     /translation="MKTLKILSAALLATFAVAAQAGPFGGPGTGYGPGPGAGVGPGAG
                     FGPGGGYGHRGGAMLERLKAADTNSDGVISREEAQAALPGLAANFDAIDANKDGNITY
                     AEMQAARGTFGPAGRGEGWKKWDVNGDGKLSRDEVANAPRLLERFDALDANHDGFLAT
                     EEMQAARLARGPGARGMGWTRLDADGDGKLSRAEVANAPRLSQEFDAIDADKDGFLTI
                     EELQAAHSHFGGRGWGRHFRG"
     gene            5984..6664
                     /gene="tsaB"
                     /locus_tag="VFV55_02215"
     CDS             5984..6664
                     /gene="tsaB"
                     /locus_tag="VFV55_02215"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX4943136.1"
                     /translation="MNLLAIETSTELCSAALLRGDELFVEEILAPNQHAERLAPMIRR
                     LLSRAGLAIAELDAFAFGQGPGSFTGLRIACGMAQGLALGASRPVVPVPSLVALAEQA
                     NASRVLVGLDARMGETYLAAYSRMGGDWEAAIEPGLHSQASLPALPGRDWVATGSGFD
                     AFGWLRSAYAPQVGHRIENDLPRAGSVARVAARRLARGEALAAEEAAPLYLRDKVALT
                     TRERQAAK"
     gene            6679..>7001
                     /locus_tag="VFV55_02220"
     CDS             6679..>7001
                     /locus_tag="VFV55_02220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4943137.1"
                     /translation="MQLNYRRMCPADLGVVADLEKSLYAFPWSLGNFRDSVNAGYDCW
                     VACSGDHVIGYAILMVALDEAHLLNFAVATGWQNQGIGRAFLDHMLGTARESKCQIVY
                     LEVRPS"
BASE COUNT         1084 a         2405 c         2399 g         1113 t
ORIGIN      
        1 ccgccatttc gccgccggcg gatctttcga gcgcgcgctc gagggctggc gcgtccggcc
       61 ccagcagctc gaattcgccg aggccgtgct cgcagccatc gaggcgtcgg ccgtgctgat
      121 cgcggaggcg ggaaccggca ccggcaagac tttcgcctat ctcgccccgg cgctcatcgc
      181 cggcggccgc gtcatcgttt cgacgggaac caagacgctg caggaccagc tgttccaccg
      241 cgacctgccg ctggtgcgcg atacgctggg cgttcccgcc cgcctcgcgc tcctcaaggg
      301 ccgctcgaac tacgtctgcc tgcaccacgt cgaggagtcg tcggcggaag gcaccttcgc
      361 ctcgcgggaa gaggcgaggg acatccacgc catcgtgcgc ttcgccgggc gctcggccac
      421 cggggacaag gcggaatgcc tggaggtctc cgagctgtct ggcgcctggg cgcgtgccac
      481 ctcgacgcgc gagaattgcc tgggctcgaa gtgccgtcat tacgaggact gcttcgtgat
      541 gaaggcgcgc aaggccgcca acgacgccga tctcgtggtc gtcaatcatc acctcttctt
      601 cgccgacgtc gtgctgcgcg acgagggcgt gaaggacctc ctgcccagcg ccaataccat
      661 catcttcgac gaggcgcacc acctcccgga tctcgcgcgg ctcttcttcg gccaggcggt
      721 ctcgacggcg caggtcatcg agctggcgcg agatgcgcga tcggcacaga tcctgcacgc
      781 acgggaaagc cccgcgatcg gcgaggccgc ccaggcggcg gaccgtgcgg cgcgcgacgt
      841 ccgcatcgcg ctgggcacgg gtaccggccg cttcggtgcc gcgcaattcg aggccaatgc
      901 cgacctcgcg aaggcgctcg atgccctcga cgagcgcctc ggcgcgctgg gcggtctcct
      961 tgcggcgcag gccgagcgag ccgacgaatt gcgcagcgcg cacacgagga cggtgctgct
     1021 gcgcgatgcc atcgccggat ggcgaggcga ggcgggcgaa ggcgcggtgc gctgggtgga
     1081 ggcctatgcg caatcggccg tgctgaaccg gactccgctc gacgtggggc cgatcttcgc
     1141 gcagcaactc gccggcgaga aacgcgcctg gatcttcacc tccgcgaccc tttccgtcgc
     1201 gggtgatttc cgtcactacc aatccgagct gggcctgcac gaggcgacga cacgccactg
     1261 ggagagcccc tacgatttcg cgaaccaggc attgctctac gtgcccgagg gcctgccgga
     1321 acccaacagt cccggccaca ccgaggccgt gatcgatgcc gcctttccgg tcatcgtggc
     1381 ggcgaaaggg cgtgccttcc tcctgttcac gagcctgcgc gccatggacg tcggccatgc
     1441 ccggctcaag gagctgctcg ccagcgccgg tctcgactgg ccggtgtacc tccagggaac
     1501 ggccggcaag aacgaactcc tcgagcggtt ccgcgatacg gcgaatgcca ttctcgtcgg
     1561 cagccagtcg ttctgggagg gcgtggacgt gaagggcgag gcactctcgc tcgtggtgat
     1621 cgacaagctg ccgttcaacc cgccggacga tccggtgctc gcggcgcgca tcgaccagat
     1681 caaccgcgcc gggggcaatg ccttcatgga gtaccaggta ccgcgcgcgg tgatcgccct
     1741 caagcagggg gccgggcgcc tcatccgcga cgagacagac cgcggcgtgc tcatgatctg
     1801 cgacccgcgg ctcgtgtcga aaccttatgg caagcgcatc tggcgcgcgc tgccgccgtt
     1861 tcgccgctcg cgcagccgcg acgacgcggt cggcttcttc accctgtccg gctgatcagg
     1921 gcgccgggtt gtaatccggc cgcacgaggc gccatccgcc cgggcacgta cgcacgtgcg
     1981 ggtaccacgc cttggccgag tcgcaatagt tgaggtgcaa cgggcgctgc gtggggccgc
     2041 cttcctcgcc gggggcagcg ggcgccacct cggtcgtggc cggggcgggc tcgcctcgcg
     2101 actgccgctc gcgccagcaa tcctcgtcct gctcgcaggc atagccgtag ctgcgataga
     2161 caggcccgta gccgtagtag ggcgggtagt acgcagggcc ccagtaccac gggtcgtacc
     2221 agcccagggc ccacggcacg ccaaaggcga ggccgaggcc ccatccccag tgcgagtgcg
     2281 agcggtggtc ccaattgccc ccgttccatc gtgcgcccga tcctggccga tgattgcccg
     2341 agtgggactg gccgaaaccg ccccgatgcg ccggggcggc gccggaaaaa cggggacccg
     2401 aactggatgc gccggaagac ctggagttgg ctgccgcggg agcttcggaa atgccggcca
     2461 gacctgcggc ggcgaggcag gcaataaggg cgattcgcat cggattcatg gcatctcctg
     2521 gttcgcgaat catggggact accctgataa caacgtgcca ggcttgcggg ggttgacgcc
     2581 tagcgggact tcttcttcct cgccggggcc gctgccgcct tctgcggggc gccttccccg
     2641 ggtttgggct ggtcgcccag cgtcgtcttc acgggacaga tggaaaaagc ggggcacttc
     2701 ttgcagccga cgacgatcgc gatcggacag acggtcatgg gatctcctct ggaaagcccc
     2761 gactctacca ccgcatgatg cgcccctacg gcgcgagggg aaacgcaacc gcggcggcga
     2821 ggcccccgcc ttcgcgcggg gcgaggtcga agcgtgcccc gtgcgccttg gcgatgcgcg
     2881 ccacgatggc gaggccgagg cccgcgcccg agcgcccgct gcgagcctcg tcgcggcgcg
     2941 tgaagggttc cttcagtcgc tcgatgtcct gtgccggaat cccgggaccg cggtcgagca
     3001 ccgcgagcgt cacctcgccg ccggtcgccg tggtgcgcac gatgatctcg ccgccaccgt
     3061 gcctcgcggc gttctggatg aggttgtcca ccagtcgctg cacggcaagc gtgcgcagca
     3121 tcagaaccgg cgtctcggcg agttcgcact tcacctgcgc gccggcgcgc gccgcccgct
     3181 ccgcgcaagc cctggcgagg gacgacaggt ccacgggcgc gtacgtctcg gtcgcctcgc
     3241 ttctcgcgaa atcgatgaac tggtcgatga tcgcgttcat gtcctcgatg tccgccacca
     3301 tgccgttctg cgtgccggcc tcgacgcggc cctcgagcat ctccacgtcg aggcgcagcc
     3361 ttgccagcgg cgtgcgcagg tcatgggaga ttccggcgag gaaggtggtg cgctcgcgct
     3421 gctcgttctt caggctttcc ttcatcaggt tgaaggccct cgccacggcg cgcacctcgg
     3481 acgggccggt ctccggcacg ggcggcggat cgccgccctt gccgaggtcg tccgccgcgc
     3541 gggcgaggcg cgtgagcggg cggttcaggc gccaggcgat gagggcagtg gcgaggatcg
     3601 cgatggcgac cccggccagc atccagtaga gaagcgcgtc cgtgttgtcg cggtcgaccc
     3661 ggttgcgcgg aaacgccacc cagaagtcgc ggccacccgc gtgcagcctg acccagaaca
     3721 cgccaggcac gccgggccgc acgaagactt ccgtgtcgtc tccgaacagg cccttgatgc
     3781 gctcgcgaaa catgtgcatg cccggcatgt cggcggcgag gcgtcccggc tcgttgccgc
     3841 gcgcggggac gacgctgatg ccttccttct cggcaagctg ctcgaagaat tcgcgctccg
     3901 ctgccggtgg cagggtattg agggcggcgt ggatcgtctt gaggtggctc acgaactgcc
     3961 cgatcctcat tgcctgccgg ggttgctgga cctgctgccg gaaaagccag aacgaggccg
     4021 cctgggaagc caccagcgcg atgaccagca ccgcgagcgt gcgccagaag aacgatcccg
     4081 cccaccattt catttctgcg gcgtcccgtc gggaatgaac acatagccga atccccacac
     4141 ggtctggatg tagcgcgggc tctgcgggtc ctcgccgagg agcttcctca gccgcgagac
     4201 ctgcacgtcg atcgagcggt cgaaactctc gtgctcgcgc ccgcgcgcca ggtccatgag
     4261 cttgtcgcgc gaaagcggct cgcgcgggtt ttcggcgaag accttgagca ccgaaaactc
     4321 gcccgtcgtg agtgcgagcg gcgcgccgtc cttcgtgagg gcgcgcgtgc cgaggttcag
     4381 cgcgaacggg ccgaaggtga cgacggtgtc ctcgcgcgcg ggcgcgcccg gcgccggcgg
     4441 tggcgcctgc cggcgcatca cggcgttgat gcgcgccacg agttcgcgcg ggttgaacgg
     4501 cttggcgagg tagtcgtcgg cgcccatctc gaggcccacg atgcggtcca cgtcgtcgcc
     4561 cttggcggtg agcatgatga cgggaagctc gttgccggcg gcgcgcaggc gccggcagat
     4621 ggcgagcccg tcctcgcccg gcagcatgag gtcgaggacc accaggtcgt agcgcgtgcg
     4681 cttcaggttg tgatccatgg ccgccccgtc gggaacggtc tcgacgccga atccctgctc
     4741 ggtcaggtag cgcttgagga ggtcgcgcag gcgcaggtcg tcgtcgacca cgaggatgcg
     4801 gtgtctgggc agggtgggca tggggtccat cgtaccggcg gcagtgcgcg cgccgccggc
     4861 cctttgtaat gagatgttaa cagcgcggac ccatgcggcc tttcgcttac aaccggttac
     4921 caccgcgagg ggctccggca acgaattacg gcgttcagga ggataggctc gagtcgcatg
     4981 caacaaggcc tttccgaaac caccctggag aacaccatga aaaccctcaa gatcctttcc
     5041 gcagccctcc tcgccacctt cgccgtcgcg gcgcaggccg gccccttcgg cggtcccggc
     5101 acaggctacg gccctggccc cggcgctggc gtcggccctg gtgccggttt cggccccggg
     5161 ggcggctacg gccatcgcgg aggcgcaatg ctcgaacgcc tgaaagctgc cgatacgaac
     5221 agcgacggcg tcatcagccg cgaagaagcc caggccgccc tgccggggct cgctgcgaat
     5281 ttcgacgcga tcgacgcgaa caaggacggc aacatcacct atgccgaaat gcaggcggcc
     5341 cgtggcacct tcgggccggc agggcgcggc gaaggctgga agaagtggga cgtcaacggc
     5401 gacggcaagc tgagccgcga cgaagtggcg aacgccccgc gccttctgga gcggttcgat
     5461 gcgctcgacg cgaaccatga cggcttcctc gccaccgagg aaatgcaggc tgctcgcctg
     5521 gctcgcgggc cgggcgcgcg cggcatggga tggacgcgcc tggacgccga cggcgatggc
     5581 aagctcagcc gcgccgaggt agccaatgct ccgcgacttt cacaggagtt cgatgcgatc
     5641 gacgccgaca aggacgggtt cctgacgatc gaggagctgc aagccgcaca cagtcacttc
     5701 ggtggtcgcg gctggggccg ccacttccgg ggctgatcgc cgatacaagg actgcccgcc
     5761 aggctcgccc ggatcgcatt cctatccggc gcgagcctgg tgggcctttt gctcgcggaa
     5821 taggggtccc gatgcattcg ccggaaccac tcgggtgccc gcacctaatc tcgataacac
     5881 tttaataatc tctaataaca tattgtttat tttataagta acgtcccggg caggcgatcg
     5941 cacagcccgg tataatgggc gcacttctcc tgcgtaattg cccgtgaacc tcctggcgat
     6001 cgagacctcc acggaactct gctcggccgc cctcctgcgt ggcgacgaac tgttcgtgga
     6061 agagatcctc gcccccaacc aacacgccga gcgcctcgcc ccgatgatcc ggcgactgct
     6121 gtcccgcgcc ggcctggcga ttgccgaact cgatgcgttc gcgttcgggc agggtccggg
     6181 gtcgttcacc ggcttgcgca tcgcctgcgg gatggcgcag gggctggcgc tcggcgcctc
     6241 ccgcccggtc gtaccggtcc catccctggt cgcacttgcc gagcaggcca acgcgagtcg
     6301 cgtgctggtg gggctggacg cccgcatggg cgagacctac ctcgcggcct attcgcgcat
     6361 ggggggcgac tgggaggccg ccatcgagcc cggtctccat tcccaggcgt ccctgccggc
     6421 ccttccgggg cgtgactggg tggccacggg tagcggtttc gacgccttcg gctggcttcg
     6481 ctcggcgtac gccccgcaag tcggccatcg gattgagaac gaccttccca gggcagggtc
     6541 cgtggcgcgc gtcgcggcac gaaggctggc ccgcggcgag gcgctcgcgg ccgaggaagc
     6601 cgcgccccta tacttgcgcg acaaggtcgc cttgacgaca cgcgaaaggc aggctgcgaa
     6661 gtgagcgcac taccccgggt gcagctgaac tatcggcgca tgtgccccgc cgacctgggc
     6721 gtggtcgccg acctcgagaa gtccctctat gccttcccct ggagtctcgg caatttccgc
     6781 gattcggtga acgcgggcta cgactgctgg gtcgcctgca gcggcgacca cgtgatcggg
     6841 tatgcgatcc tcatggtcgc gctcgacgag gcgcacctcc tcaacttcgc cgtcgcgaca
     6901 ggttggcaga accagggcat cgggcgtgcc ttcctcgacc acatgctggg cacggcgcgg
     6961 gagtcgaagt gccagatcgt gtacctcgag gtgcgaccgt c
//
LOCUS       DASUEV010000069         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1271374, whole genome shotgun sequence.
ACCESSION   DASUEV010000069 DASUEV010000000
VERSION     DASUEV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271374"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /gene="rpsC"
                     /locus_tag="VFV55_02225"
     CDS             <1..495
                     /gene="rpsC"
                     /locus_tag="VFV55_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX4943138.1"
                     /translation="PGVVIGKKGEDIEGLRHVLQKLMGVPVHVNIEEIRKPELDAQLI
                     ADSITGQLEKRVMFRRAMKRAMQNAMRLGAQGIKITSAGRLNGAEIARTEWYREGRVP
                     LHTLRADIEYGFSEAHTTYGTIGVQVLVFKGEVLGKGEQPVVAAPEAERKTRKPGVKH
                     VAAS"
     gene            479..892
                     /gene="rplP"
                     /locus_tag="VFV55_02230"
     CDS             479..892
                     /gene="rplP"
                     /locus_tag="VFV55_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX4943139.1"
                     /translation="MLQPARRKYRKEQKGRNKGVATNGAKVAFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRIFPDKPISKKPAEVRMGNGKGSPEYFVAEIVPGKV
                     LYEMDGVEEALAREAFALAAAKLPIRTTFVQRLIG"
     gene            903..1103
                     /gene="rpmC"
                     /locus_tag="VFV55_02235"
     CDS             903..1103
                     /gene="rpmC"
                     /locus_tag="VFV55_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX4943140.1"
                     /translation="MKAIELRTKSVEDLQKELLELRKAQFGLRMQVATQSLTKNSEIA
                     RVKREIARVHTVLTEKAKASKQ"
     gene            1100..1366
                     /gene="rpsQ"
                     /locus_tag="VFV55_02240"
     CDS             1100..1366
                     /gene="rpsQ"
                     /locus_tag="VFV55_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX4943141.1"
                     /translation="MSETAKNQRQLTGKVVSDKMEKTVTVLVERQVMHPVIGKVVRRT
                     RKYHAHNEGNEAKAGDTVLIVECRPISKTKSWKVAKLVERARLE"
     gene            1641..2009
                     /gene="rplN"
                     /locus_tag="VFV55_02245"
     CDS             1641..2009
                     /gene="rplN"
                     /locus_tag="VFV55_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX4943142.1"
                     /translation="MIQMQSILDVADNTGARSVMCIKVLGGSKRRYAGIGDIIKVSIK
                     SAAPRGRVKKGEVYSAVVVRTAHGVRRPDGSVIKFDTNAAVLLNPKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            2020..>2309
                     /gene="rplX"
                     /locus_tag="VFV55_02250"
     CDS             2020..>2309
                     /gene="rplX"
                     /locus_tag="VFV55_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX4943143.1"
                     /translation="MKKIRKGDQVVVTTGKDKDKRGTVLRVLDTGKVVVEGINRVKRH
                     TRPNPVKGQTGGIVDKEMPIQASNVALFNPATGKGDRVGIRVLEDGRKVRFF"
BASE COUNT          484 a          736 c          789 g          300 t
ORIGIN      
        1 ccgggcgtgg tgatcggcaa gaagggcgag gacatcgagg gcctgcgcca cgtcctgcag
       61 aagctcatgg gcgtgccggt gcacgtgaac atcgaggaga tcaggaagcc cgagctggac
      121 gcccagctca tcgccgactc gatcaccggc cagctcgaga agcgggtgat gttccgccgc
      181 gccatgaagc gcgccatgca gaacgccatg cgcctgggcg cccagggcat caagatcacc
      241 agcgcggggc ggctgaacgg cgccgagatc gcgcgcaccg agtggtaccg cgaaggccgc
      301 gtgccgctgc acacgctgcg cgccgacatc gagtacggct tctcggaggc gcacacgacc
      361 tacggcacga tcggcgtcca ggttctcgtc ttcaagggcg aggtcctcgg caagggcgag
      421 cagcccgtcg ttgcggcgcc ggaagcggaa cgcaagacca ggaaaccggg ggtgaaacat
      481 gttgcagcca gctagaagga agtaccgcaa ggagcagaag ggccgcaaca agggcgtggc
      541 cacgaatggc gcgaaggtgg cgttcggcga gttcggcctc aaggccgtcg gccgcggccg
      601 cctcaccgcc cgccagatcg aggcggcgcg ccgcgccatg acccgccaca tcaagcgcgg
      661 cggccggatc tggatccgca tcttcccgga caagccgatc tccaagaagc ccgcggaagt
      721 ccggatgggc aacggcaagg gctcgcccga gtacttcgtg gccgagatcg tcccgggcaa
      781 ggtgctctac gaaatggacg gcgtggagga ggcgctggcg cgggaggcgt tcgcgctcgc
      841 cgcggcgaag ctcccgatcc gcacgacctt cgtgcagcgc ctcatcggtt gaaggaactg
      901 acatgaaagc gattgaactc cgtaccaaga gcgtcgagga cctgcagaag gaactgctgg
      961 agctgcgcaa ggcacagttc ggcctgcgca tgcaggtggc cacgcagagc ctcaccaaga
     1021 acagcgagat cgcgcgcgtg aagcgcgaga tcgcgcgcgt gcacaccgtg ctcaccgaga
     1081 aggcgaaagc gagcaagcaa tgagcgaaac cgcgaagaac cagcgccagc tcacgggcaa
     1141 ggtcgtgagc gacaagatgg aaaagaccgt gaccgtcctg gtcgagcgcc aggtgatgca
     1201 cccggtgatc ggcaaggtgg tccggcgcac ccgcaagtac cacgcccaca acgagggcaa
     1261 cgaggcgaag gccggcgaca ccgtgctgat cgtcgagtgc cggccgatct cgaagaccaa
     1321 gtcgtggaag gtcgccaagc tggtcgaacg cgcgcgactg gagtaggctt gcgccaggcg
     1381 gcgaaaagct gctagaatca acgtctttgc tacttcctgc cgtggcttcg tcggcaggaa
     1441 atccgattaa accccgggct cgacccgggg gccacccggg cgtcggccga agccgatgca
     1501 cccaagcgcc cgggcccaga ttcagttcac gccagccggt ggcgtctccg ctaaggaggt
     1561 gtcgtgccga cccaagacgg atccaagact ggccggacgg gcctcgaaac cccgccgggt
     1621 caagttggag agaagacaaa atgattcaga tgcagtcgat tctcgacgtc gccgacaata
     1681 ccggcgcgcg ctcggtgatg tgtatcaagg tgctcggagg ctcgaagcgc cgctacgccg
     1741 gcatcggcga catcatcaag gtgagcatca agtcggccgc cccgcgcggc cgcgtgaaga
     1801 agggcgaggt ctacagcgcg gtggtggttc gcaccgcgca cggcgtccgc cgccccgacg
     1861 gctcggtcat caagttcgac acgaatgccg cggtgctgct caacccgaag ctcgagccga
     1921 tcggcacgcg catcttcggg cccgtgaccc gcgagctgcg cacggagcgc ttcatgaaga
     1981 tcgtctcgct ggcgccggaa gtgctgtagg ggaccccaca tgaaaaagat acgcaagggc
     2041 gaccaggtgg tcgtcaccac cggcaaggac aaggacaagc gcggcaccgt gctgcgcgtc
     2101 ctcgacacgg gcaaggtcgt ggtcgagggc atcaaccgcg tgaagcgcca cacccgtccc
     2161 aaccccgtca aggggcagac aggcggcatc gtcgacaagg aaatgccgat ccaggcctcg
     2221 aacgtggcgc ttttcaatcc cgccaccggc aagggcgatc gcgtgggcat ccgcgtcctc
     2281 gaggacgggc ggaaggtccg cttcttcaa
//
LOCUS       DASUEV010000070         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_353527, whole genome shotgun sequence.
ACCESSION   DASUEV010000070 DASUEV010000000
VERSION     DASUEV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353527"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFV55_02255"
     CDS             <1..236
                     /locus_tag="VFV55_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943144.1"
                     /translation="DGQVIGIVTSKLNALAVQRSTGDLPQNISFGVRGEIAQAFLQAH
                     AARFASGAGGRKLENTEVAAIGRSVTLLVACRR"
     gene            295..1368
                     /gene="hppD"
                     /locus_tag="VFV55_02260"
     CDS             295..1368
                     /gene="hppD"
                     /locus_tag="VFV55_02260"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX4943145.1"
                     /translation="MGTDGFEFIEYAAPDPRAMGALFERLGFKSIARHRHKDVLLYRQ
                     GEINFIVNAEPDSFAQRFARQHGPSICAIAFRVADAKAAYERAVDLGAWGYAGHAGPG
                     ELNIPAIKGIGDSLLYFIDRWRGKGGKETGDIGNIGFYDVDFEPLQGTKSTAGSLNPV
                     GHGLTVIDHLTHNVHKGRMKEWADFYERLFNFREIRYFDIEGQATGVKSKAMTSPCGK
                     IRIPINEEGTEKAGQIQEYLDKYHGEGIQHVALGSVDLLRTVDALKAKGVKLLDTNDT
                     YYTLVDKRIPGHGEDLAALKQRKILIDGKAGALLLQIFSENQLGPIFFEFIQRKGDEG
                     FGEGNFKALFESIELDQMRRGVL"
     gene            1365..2210
                     /locus_tag="VFV55_02265"
     CDS             1365..2210
                     /locus_tag="VFV55_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4943146.1"
                     /translation="MSVAHEPGRCNSADVELSYRRVNRGQSPILFVHGLSYFSWDWLE
                     AAAALQGDCVCLDMRGFGDSAWAKDYSVPAMASDLGAVMDHLGWQKAAIVGHSMGGRN
                     SAYFAAKNPQRVSGLVLVDYSPENAPPGSKRVATSVAGVPDVFATIDEAMAYFKAGPE
                     KRARYEAYLKVVQGGYAIKRDTHFRDQFRQALATGERAKLGVDMWQVLGELRVPTLVL
                     RGTRSDIFAPETVEKVKAANPRIQLAQVDGGHNLAVDNLPGFLAAVRPFIASLQESNH
                     AHARH"
     gene            2191..>2676
                     /locus_tag="VFV55_02270"
     CDS             2191..>2676
                     /locus_tag="VFV55_02270"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4943147.1"
                     /translation="MHTRGIDHLAIVSDDMPTALDFYTRVMGFQLVHARRVPFEADRW
                     QPPYDNLRHYFFDMGNDSLFAVFEYPKGLSRQVRDHVGGMQHVAFHVPPDKFDAMIER
                     VKGCGVHVIGPVPLGGRFWSAYFLDPFGIRLEFATDRSGAGRSVIESVRQTEAEARAE
                     LE"
BASE COUNT          469 a          895 c          885 g          427 t
ORIGIN      
        1 aggacggcca ggtgatcggc atcgtgacct ccaagctcaa cgcgctcgcg gtgcaaagga
       61 gcaccggcga cctgccgcag aacatcagct ttggcgtgcg cggcgagatc gcgcaggcgt
      121 tcctccaggc gcacgcggcg cgcttcgcca gcggcgcggg cgggcgcaag ctcgagaaca
      181 ccgaagtcgc ggcgatcggc cgctcggtga cgctcctggt cgcctgccgc cggtgacaat
      241 cggggtcaga gcccgattta tacttgcatt atggccgagc gctgggaaaa cccgatgggc
      301 accgacggct tcgagttcat cgaatacgcc gcgcccgatc ccagggccat gggcgccctc
      361 ttcgagcgcc tgggcttcaa gtccatcgcg cggcaccggc acaaggacgt gctgctctat
      421 cgccagggcg agatcaattt catcgtcaac gccgagcccg attcgttcgc gcagcgcttc
      481 gcgcgccagc acgggcccag catctgcgcc atcgcatttc gcgtcgccga cgccaaggcc
      541 gcctatgagc gcgcggtgga cctcggcgcc tggggctacg ccgggcacgc cgggccgggc
      601 gagctgaaca tcccggcgat caagggcatc ggcgactcgc tgctctactt catcgaccgc
      661 tggcgcggca agggcggaaa ggaaaccggc gacatcggca acatcggctt ctacgacgtg
      721 gacttcgagc cgctgcaggg aacgaaatca acagccgggt cgctcaaccc cgtgggccac
      781 ggcctcacgg tcatcgacca cctgacgcac aatgtgcaca aggggcgcat gaaggagtgg
      841 gcggacttct acgagcgcct gttcaatttc cgcgagatcc gctatttcga catcgagggc
      901 caggccacgg gcgtgaagtc gaaggcgatg accagcccct gcggcaagat ccgcatcccg
      961 atcaacgagg aaggcaccga gaaggcgggg cagatccagg agtacctcga caagtaccac
     1021 ggcgagggca tccagcacgt ggcgctgggc tccgtggacc tgttgcgcac ggtcgacgcg
     1081 ctgaaggcga aaggcgtgaa gctcctcgac accaacgaca cctactacac gctggtggac
     1141 aagcgcatcc cggggcacgg cgaggacctg gcggcgctca agcagcgcaa gatcctgatc
     1201 gacggcaagg cgggcgcgct gctgctgcag attttctccg agaaccagct cgggccgata
     1261 tttttcgagt tcatccagcg caaaggcgat gagggtttcg gcgagggcaa cttcaaggcg
     1321 ctgttcgagt ccatcgagct cgaccagatg cgccggggag tgctgtgagc gtcgcccacg
     1381 agcccggccg ctgcaattcc gcggacgtcg agctctccta ccgccgggta aatcggggtc
     1441 agagcccgat tttgttcgtc cacgggcttt cctatttttc ctgggactgg ctcgaggcgg
     1501 ccgccgcgct ccagggcgac tgcgtgtgcc tggacatgcg cggcttcggg gattccgcct
     1561 gggcgaagga ttactcggtg ccggcaatgg caagcgacct tggcgcggtg atggatcacc
     1621 tcggctggca gaaagcggcc atcgtgggcc actcgatggg tgggcgcaac tccgcttact
     1681 ttgcggcgaa gaatccgcag cgcgtctcgg gcctcgtgct ggtggactat tcgcccgaga
     1741 acgcgccgcc cggctccaag cgcgtggcaa ccagcgtcgc cggtgtgccg gacgtcttcg
     1801 ccacaatcga cgaggcgatg gcgtacttca aggccggccc tgagaagcgc gcacgctatg
     1861 aggcctactt gaaggtcgtc caggggggct atgccatcaa gcgcgatacg cacttccgcg
     1921 accagttcag gcaggccctc gccaccggcg agcgcgcgaa gctcggcgtg gacatgtggc
     1981 aggtactcgg cgaactgcgc gtgcccacgc tcgtgctgcg cggcacgcgc tcggacattt
     2041 tcgcgcccga aaccgtcgag aaggtgaagg ccgccaaccc gcgcatccag cttgcgcaag
     2101 tggacggcgg ccacaatctc gcggtggaca acctgcccgg cttcctcgcc gccgtgcgcc
     2161 ctttcatcgc ttcgctgcag gagagcaacc atgcacacgc gcggcattga ccacctcgcc
     2221 atcgtctcgg acgacatgcc cacggcgctg gacttctaca cccgcgtgat gggtttccag
     2281 ctggtgcacg cgcgccgcgt gcccttcgag gccgaccgct ggcagccgcc ctatgacaac
     2341 ctgcgccact acttcttcga catgggcaac gactcgctgt tcgccgtgtt cgagtacccc
     2401 aaaggcctgt ccaggcaggt gcgcgaccac gtcggcggca tgcagcacgt cgccttccac
     2461 gtgccgccgg acaagttcga cgccatgatc gagcgcgtga agggttgcgg cgtgcacgtc
     2521 atcggcccgg tgccgctcgg cgggcgcttc tggtcggcgt atttcctcga ccccttcggc
     2581 atccgcctgg agttcgccac cgaccgcagc ggcgcggggc gcagcgtgat cgagtcggtg
     2641 cgccagaccg aggccgaggc gcgcgccgag ctggag
//
LOCUS       DASUEV010000071         8150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1271779, whole genome shotgun sequence.
ACCESSION   DASUEV010000071 DASUEV010000000
VERSION     DASUEV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271779"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /locus_tag="VFV55_02275"
     CDS             <1..1742
                     /locus_tag="VFV55_02275"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX4943148.1"
                     /translation="VLAHWSVIKPAYQVTLQDGTTLVASGDHRFLTERGWKHVTDAES
                     RVQRAHLTTANKLMGTGGFAEGPLENDDYRRGYLSGLIRGDGHLESYHYDRPGGFDHL
                     HVFRLALCDPEALLRAQDYLLDFEIATQEFQFAYASASRRPMHAIRTSALAKVDAIRR
                     LIDWPTSPRREWSAGFLAGIFDAEGSYSKAVLGISNTDTEIINRVSDCLRTFRFDFVV
                     DHVHRELTKPIDVVRVRGGLGEHLRFFHTVGPAITRKLDISGQAVKSDAKLGVVSIEP
                     LGAAMRLFDIMTETEDFIADGVVSHNCYARPSHAYVGLSPGLDFETRLFSKPNAAELL
                     RDELARPGYRCEPIMIGANTDGYQPIERSERVTRSIVEVLAECRHPVTIITKSALVER
                     DLDLLVPMARDGLAHVVISVTTLDHDLARRMEPRASAPARRIQAIARLSAAGVPVGVN
                     VAPVIPFLTDAEVESILEAAAAAGARSAGYTLLRLPWEVRDLFKAWLAEHFPLKAAHV
                     MARVREMRDGRENDPEFGSRMHGTGELSALLSQRFGKACKRLGLAHGHRADPSLDTSK
                     FRPPRSRDQLDLF"
     gene            1844..5362
                     /locus_tag="VFV55_02280"
     CDS             1844..5362
                     /locus_tag="VFV55_02280"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX4943149.1"
                     /translation="MSALPVTLEDKYALASGRVFITGTQALVRLAMMQRDRDRAAGLD
                     TAGFISGYRGSPLGGFDQALWKAKKHLESHHIHFSPGVNEELAATAVWGSQQVGLFPG
                     AKYDGVFAMWYGKGPGVDRCGDVFKHANAAGTAKHGGVLLVAGDDHACKSSTLPHQSE
                     HAFDAAMIPVLYPADVRDIIELGLHGFAMSRHSGCYVALKTVSETVDSSASIVIDPAF
                     PKIVLPDDVSLPAGGMNIRWPDAPLEQEVRLQHDKIYAALAYCRANGLNRVTIDSPRP
                     RLGIIASGKSYLDVLQALENLGIDARHAAEVGIRLFRVGMPWPLEPNGVREFAQGLDE
                     ILVVEEKRQLIEYQLKEQLYNWRDDVRPRVIGKYDEHGEWEVHRGEWLLPAAGELTPA
                     MVARVIAARIGRFYSSPIVEARLKFLEAKEAALARPRARVARIPYFCSGCPHNTSTRV
                     PEGSTALAGIGCHYMAIWIRPGETRTFTQMGGEGVPWVGMQNFTGTRHVFANLGDGTY
                     FHSGLYAIRAAVSANANMTYKILFNDAVAMTGGQPLDGSLTVAKVTRQVAAEGVGRIV
                     IVTDEPQKYVGATDLAAGTTIHHRDELDAVQRMLRELPGVTVLVYDQTCAAEKRRRRK
                     RGTYPDPARRVMINERVCEGCGDCGEKSNCLSIVPVETEFGRKRAIDQSTCNKDYSCI
                     KGFCPSFVTVEGGSLRKRKPVAREMQVPLPEPAIPVLERPWGILVTGVGGTGVVTIGA
                     LLGVAAHIEGKGVAVLDMTGLAQKGGAVYSHVRIAARPEDIHAVRIAAGEANAVIGCD
                     MIVAASDEALAKMQAGHTRAVINADVATTGAFTRDPDLQVPRLGMADAIREACGPEAT
                     DFVEAGELATALMGDSIATNLFMVGFAWQRGLVPVGREAIVRAIELNGAAVESNKAAF
                     EWGRRAVADLAAVRKAAAPDAGGQARRRLSESLDEMFARRREELVRYQGEAYAARYAE
                     RVGRVRDAESRTVPGSTALAEAVARYLFKLMACKDEYEVARLYADGDFLAQVANQFEG
                     AYRLKLHLAPPLWSKKDSSTGEPRKGTFGPWMLKAMAVLAKFKGLRGTAFDPFGYSQE
                     RREERRLMAEYEALLDEIVERLSAQTIAVSIELASLPEHIRGYGPVKARHIALVAERR
                     TALLARLRDPAQGAALKPAIPARVAA"
     gene            complement(5370..6311)
                     /locus_tag="VFV55_02285"
     CDS             complement(5370..6311)
                     /locus_tag="VFV55_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX4943150.1"
                     /translation="MADPEPRRPRPPADPATIGVFSLSGNVDPARLDEGIARLEAAGH
                     RVITAEQARHQWRYFAGTDEQRVEGFHRLLDDPAIDVMLAARGGYGITRILPAIDWAR
                     VGASGKVFAGFSDFTAFNCAALARAGLATLHGPMVAIDFGDGDPDEFMLRHFRSTLWG
                     REDSETVACDTGCPPRAIRGRLWGGNLSMLAHLVGTPYLPAIEGGLLYVEEIAEEPYA
                     IERLFLQLHHAGILARQAAILLGDFPDCVPANPARYPYAMEEVIETLRAIAPCPVLAG
                     LPFGHVARKLTLPFGMPALLSLDTDRYTLAWPGLAGP"
     gene            complement(6304..6681)
                     /locus_tag="VFV55_02290"
     CDS             complement(6304..6681)
                     /locus_tag="VFV55_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4943151.1"
                     /translation="MIDRFDHLHIQPADFDRSLAFYRDMLGWTVVSAWGDAASSRGAV
                     LSGGAIKVVLAERSKYPAQPAEPHPAVHLDIHDIDKRFEALPAGDHVVLPPGVSERGT
                     RRFVVRDPDGNLIAFEELNRQRG"
     gene            complement(6695..>8150)
                     /locus_tag="VFV55_02295"
     CDS             complement(6695..>8150)
                     /locus_tag="VFV55_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX4943152.1"
                     /translation="RMAANPLVFALANPVPEILPAEVKAVRDDAIIATGRSDYPNQVN
                     NVLCFPFIFRGALDVGATTINEPMKLAAVNAIADLAMAEQSDVVAMAYQQKEVSFGRE
                     YLIPKPFDPRLIVKIAPAVAKAAMDSGVATSPIRDFEAYAQRLNEFVYHSGHIMRPIF
                     TAARASPMRIVYAEGEDERVLRAVQVVVDEKLARPVLVGRPTVLEKRIERFGLRLNAG
                     EHFDVVNPEWDARYRDYWTEYHRLTERRGVSMQYAKIEMRRRHTLIGAMMMHKGEADG
                     MICGTFGTHRLHLHYVDQVIGLRPGVKDYYAMNILMLPKRTVFICDTYVNDDPTPAQI
                     AEMTQLAAEEIRRFGLVPRAALLSHSSFGTSDAPSAAKMRAALAIIRERMPDLDVDGE
                     MHGDAALSVEVRQAAFPNSRLKGEANLLVMPTLDAANIAFNLLKTASGDGITIGPVLL
                     GASMPAHILTPTATVRRIINMTAVAVVDAQAQRG"
BASE COUNT         1347 a         2708 c         2804 g         1291 t
ORIGIN      
        1 gtgtactcgc acattggagc gtcatcaaac cggcctatca agtcaccctg caggatggaa
       61 cgacactcgt cgcgagtggt gatcaccggt tcctgaccga gcgcggatgg aagcacgtga
      121 ccgatgccga atcgcgtgtt cagcgagcgc atctgacgac ggcaaacaag ctcatgggaa
      181 ccgggggctt tgctgaaggt ccgctcgaga acgatgacta tcggcgcggc tacctgtccg
      241 ggctaattcg gggtgatggg cacctcgagt cgtaccacta cgatcgccct ggcgggttcg
      301 accatcttca tgtttttcgc cttgccttgt gcgacccgga ggccctgttg agagcgcagg
      361 actacctcct ggactttgaa attgccacgc aggagtttca attcgcgtac gcgtctgcca
      421 gccgcaggcc aatgcatgcc atccgcacga gcgccctggc gaaagtcgat gcgattcggc
      481 gcctcatcga ctggccgacg agtccgagga gggaatggtc cgcagggttc ctggccggga
      541 tcttcgatgc agagggcagt tacagcaagg cggtactcgg gatttcgaat acggatacag
      601 aaatcatcaa ccgggtatct gactgcctga gaacctttcg cttcgatttc gttgtggatc
      661 atgttcaccg tgagttgacc aagcccatcg atgtggtacg agtgagaggt ggattgggcg
      721 agcatctgcg ctttttccac accgtcggcc cggccatcac gcgcaagctc gacatctccg
      781 gtcaagcagt caagagcgat gccaaactgg gtgtcgtaag catcgagccg ttgggcgcag
      841 caatgcgtct tttcgacatc atgacggaaa cggaggattt cattgccgac ggcgtcgtga
      901 gtcacaactg ctacgcccgc ccgtcccatg cttacgtagg cctttccccc ggcctcgatt
      961 tcgaaacccg cctcttctcc aagcccaacg ccgcagaact gctgcgcgac gagctcgcca
     1021 ggcccggcta tcgctgcgag ccgatcatga ttggcgccaa taccgacggc taccagccca
     1081 tcgagcggag cgaacgcgtg acccggtcga tcgtcgaggt gctggccgag tgccgccatc
     1141 cggtcacgat catcaccaag tcggcgctgg tcgagcgcga cctcgacctg ctggtgccca
     1201 tggcgcggga cgggctcgca cacgtcgtga tctcggtgac cacgctcgac catgacctgg
     1261 cgcgccgcat ggaaccgcgc gccagcgcgc cggcacggcg catacaggcg atcgcgcggc
     1321 tttcggcagc cggcgtgccg gtcggggtga acgtcgcacc ggtgattccg ttcctcaccg
     1381 atgcggaggt ggagagcatc ctcgaagccg cggcggcggc aggagcccgg agcgcgggct
     1441 acacgctcct gcgcctgccc tgggaagtgc gcgacctctt caaggcgtgg ctcgcggaac
     1501 atttcccgct caaggccgcg cacgtgatgg ctcgcgtgcg cgagatgcgc gacgggcgcg
     1561 agaacgatcc cgaattcggc agccgcatgc acggcaccgg cgagctttcc gccttgctct
     1621 cgcaacgctt tggcaaggcc tgcaagcggc tcgggctcgc acacgggcac cgggcggacc
     1681 cgtccctgga cacctcgaag ttccgccccc cgcgcagccg cgaccagctc gatcttttct
     1741 gaagttcgcc cgcgtatact cttgcccggt ccggtgccgg ggaacacaat ttcccgggac
     1801 ggcgcgcgca acgccccaag agcacgactt tcgaggagcc cgcatgtccg cattgcccgt
     1861 cacgctggaa gacaagtacg cgcttgcctc cggccgcgtc ttcatcaccg gtacccaggc
     1921 actggtcagg cttgcgatga tgcagcgcga ccgcgaccgc gcggcgggcc tcgacaccgc
     1981 gggcttcatc tccggctacc gcggctcgcc cctgggcggc ttcgaccagg ccctgtggaa
     2041 ggcgaagaaa cacctcgagt cgcaccacat ccacttctcg ccgggcgtga acgaggaact
     2101 cgcggccacg gcggtgtggg gctcgcagca ggtggggttg tttccgggag cgaaatacga
     2161 cggcgtcttc gccatgtggt acggcaaggg gccgggcgtg gatcgttgcg gggacgtctt
     2221 caagcatgcg aacgcggcgg gcaccgcgaa acatggcggc gtgctgctcg tcgcgggcga
     2281 tgaccacgcc tgcaagtcct ccacgctccc gcaccagtcg gagcacgcgt tcgacgccgc
     2341 catgatcccg gtgctctacc ccgcggacgt gcgggacatc atcgagctgg ggctgcacgg
     2401 cttcgcgatg tcacgccact cgggttgcta tgtcgccctg aagacggtat ccgagacggt
     2461 cgactcctcg gcctcgatcg tcatcgatcc cgcgtttccg aagatcgtgc tgccggacga
     2521 cgtttccctt cccgcgggcg gaatgaacat ccgctggccg gatgcgccgc tggagcagga
     2581 agtgcgcctg cagcacgaca agatctacgc cgcgctcgcc tattgccgcg ccaacgggct
     2641 caaccgcgtc acgatcgact cgcccaggcc gaggctcggc atcatcgcct ccgggaaatc
     2701 ctacctcgac gtcctgcagg cgctggagaa cctgggcatc gacgcgcgcc atgccgccga
     2761 agtcgggatc cgcctcttcc gcgtcggcat gccctggccc ctcgaaccca acggcgtgcg
     2821 cgaattcgcc caggggctcg acgagatcct ggtggtggag gaaaagcgcc agctcatcga
     2881 gtaccagttg aaggaacagc tctacaactg gcgcgacgac gtgcgcccgc gcgtcatcgg
     2941 caagtacgac gagcacgggg aatgggaggt gcaccgcggc gaatggctgc tgccggcggc
     3001 gggggagctc acgccggcca tggtcgcccg cgtcatcgcg gcgcgcatcg gcaggttcta
     3061 ttcgtcaccg atcgtggagg cgcgcctcaa gttcctcgag gccaaggaag ccgcgcttgc
     3121 ccgcccgagg gccagggtgg cgcgcatccc gtacttctgc tccggctgcc cgcacaacac
     3181 gtcgacccgg gtgccggaag gctcgacggc gcttgcgggt atcggctgcc attacatggc
     3241 catctggatc cggcccgggg agacgaggac cttcacgcag atgggcggcg agggcgtgcc
     3301 ctgggtcggc atgcagaact tcaccgggac ccggcacgtc ttcgcgaacc tcggcgacgg
     3361 cacgtacttc cactccggcc tctacgcgat ccgcgccgcc gtcagcgcga acgccaacat
     3421 gacctacaag atcctcttca acgatgccgt cgccatgacg ggcgggcagc ccctggatgg
     3481 gtcgctcacc gtcgcgaagg tcacgcggca ggtcgccgcg gaaggggtcg ggcgcatcgt
     3541 gatcgtcacc gacgagccgc agaagtacgt cggggcaacc gacctcgcgg ccggaaccac
     3601 gatccaccac cgcgacgaac tggacgcggt gcagcgaatg ctgcgcgagc tcccgggcgt
     3661 gacggtgctg gtgtacgacc agacctgcgc cgccgagaag cgccgccggc gcaagcgcgg
     3721 gacctatccc gacccggcca ggcgcgtgat gatcaacgag cgcgtctgcg agggatgcgg
     3781 cgattgcggc gagaagtcca actgcctttc catcgtcccc gtcgagaccg agttcgggcg
     3841 caagcgggcc atcgaccaga gcacctgcaa caaggactac tcgtgcatca agggtttctg
     3901 cccgagcttc gtgacggtcg agggcgggag cctgcgcaag cgcaagcccg tcgcgcgcga
     3961 gatgcaagtg ccgcttcccg agccggcaat cccggtcctg gaacgtccct ggggcatcct
     4021 cgtcacgggc gtcgggggca ccggcgtggt cacgatcggg gcgctcctcg gcgtggccgc
     4081 gcacatcgaa ggcaagggcg tggcggtcct ggacatgacg gggcttgccc agaagggcgg
     4141 ggccgtctac tcgcacgtcc gcatcgccgc gagaccggag gacatccatg cggtgcgcat
     4201 cgccgcgggc gaagccaatg cagtcatcgg ctgcgacatg atcgtggccg cctccgacga
     4261 ggccctggcg aagatgcagg ccggccatac gcgtgccgtc atcaatgccg acgtcgcgac
     4321 cactggcgcg ttcacgcgcg atccggatct ccaggttccg cgtctcggca tggccgacgc
     4381 gatccgcgaa gcctgtggcc ccgaggcgac cgatttcgtc gaggccggcg agcttgcaac
     4441 ggcgctcatg ggcgattcca tcgcgaccaa cctcttcatg gtgggcttcg cgtggcagag
     4501 gggcctcgtt ccggtggggc gcgaggccat tgtccgggct atcgagctca acggcgcggc
     4561 ggtcgagtcg aacaaggcgg cgttcgaatg gggccggcgc gcggtggcgg atctcgcggc
     4621 cgtgcgcaag gcggctgcac cggatgccgg ggggcaggca agacgccggc tgtcggaaag
     4681 cctggacgaa atgttcgcgc gccgccgcga ggagctcgtc aggtaccagg gcgaggccta
     4741 cgccgcgcgc tacgccgagc gagttgggcg cgtccgcgat gccgagtcga ggacggtgcc
     4801 gggatcgacc gcgcttgccg aggcggtcgc gcgctacctt ttcaagctga tggcctgcaa
     4861 ggacgaatac gaggtggcac ggctatacgc cgacggcgac ttcctcgcgc aggtggcgaa
     4921 tcaattcgaa ggcgcctacc gcctcaagct gcacctcgcg ccgccgctgt ggtcgaaaaa
     4981 ggactcgtcg accggggagc cgcgcaaggg caccttcggg ccgtggatgc tgaaggccat
     5041 ggccgtgctc gcgaagttca aggggctgcg agggaccgcg ttcgacccgt ttggctactc
     5101 gcaggagcgg cgcgaggagc gccggctcat ggcggaatac gaggcgctgc tggatgaaat
     5161 cgtcgagcgc ctttccgcgc agacgattgc cgtttccatc gaactcgctt ccctgccgga
     5221 gcatatccgc ggttacgggc cggtaaaggc gcgccatatc gcgctggtcg ccgagcgccg
     5281 gacggcactc ctcgcgcgcc tgcgcgaccc ggcgcagggc gcagccctca agccggcgat
     5341 accggcccgc gtggcggcct gacgcgcatt cagggtccgg cgagccccgg ccacgccagc
     5401 gtgtaccggt ccgtgtccag ggagagcagg gccggcatgc cgaagggcaa ggtgagcttc
     5461 ctcgcgacat ggccgaacgg caggcccgcg aggaccgggc atggcgcaat cgcgcgaagg
     5521 gtctcgatga cctcttccat cgcgtacgga taacgcgccg ggttcgcggg cacgcagtcg
     5581 ggaaagtcgc ccagcaggat ggcggcctgc cgcgcgagga tccccgcatg gtggagttgc
     5641 aggaagagcc gctcgatggc gtacggttcc tccgcaatct cctccacgta aagcaggccc
     5701 ccttcgatgg ccggcaggta cggcgtgccg acgagatggg cgagcatcga gaggttgccg
     5761 ccccagagcc gcccgcggat cgcgcgcggc gggcagcccg tgtcgcaggc gacagtctcg
     5821 gaatcctcgc ggccccacag ggtggacctg aaatggcgga gcatgaattc gtcgggatcg
     5881 ccgtcgccga aatcgatcgc gaccatcggg ccatgcagcg tggcgagccc ggcgcgcgca
     5941 agggccgcgc aattgaaggc ggtgaaatcg ctgaaacccg cgaacacctt gccgctggcg
     6001 cccacgcggg cccagtcgat cgccggcagg atccgcgtga tcccgtagcc tccgcgggcg
     6061 gcgagcatca cgtcgatggc cgggtcgtcc agcaaccggt ggaatccctc gacgcgctgc
     6121 tcgtcggtgc cggcgaagta gcgccactgg tggcgggcct gttcggcggt gatgacccgg
     6181 tggcccgccg cctcgagtcg ggcgatgccc tcatccagcc gtgcggggtc gacattgccc
     6241 gacagcgaga agacgccgat ggtagcgggg tcggccgggg gccgcggccg ccgcggctcg
     6301 ggatcagcca cgttgccggt tcagctcttc gaaggcgatg aggttgccgt cggggtcgcg
     6361 aaccacgaac cgtcgcgtgc cacgctcgct cacgcccggc gggaggacca cgtggtctcc
     6421 cgcgggcagc gcctcgaagc gcttgtcgat gtcgtggatg tcgaggtgga ccgccggatg
     6481 gggctccgca ggctgcgccg gatacttcga gcgctcggca aggaccacct tgatcgcgcc
     6541 gccggacagg accgccccgc ggctcgaggc cgcatcgccc cacgcggaca ccaccgtcca
     6601 gccgagcatg tcgcgataga acgcgagcga ccggtcgaaa tcggctggct ggatatggag
     6661 gtggtcgaaa cgatcgatca tcgggccggg ggcgctagcc gcgttgcgcc tgcgcgtcca
     6721 cgacggcaac ggccgtcatg ttgatgatgc gcctgacggt cgcggtcggc gtaaggatat
     6781 gcgctggcat cgaggccccg agcagcacgg ggccgatggt gatgccgtcg ccggaggcgg
     6841 tcttgagcag gttgaaggcg atgttggccg catcaagcgt cggcatgacg agcaggttcg
     6901 cctcgccctt cagccgcgaa ttcgggaacg cggcctgcct cacttcgacg gagagggccg
     6961 cgtcgccgtg catctccccg tcgacgtcga gatcgggcat ccgctcgcgg atgatggcga
     7021 gcgcggcgcg catcttggcc gccgacggcg cgtcgctggt gccgaagctg gagtgcgaga
     7081 gcagggccgc gcgcggcacc aggccgaagc gccggatctc ctccgcggcg agctgggtca
     7141 tctcggcgat ctgcgccggc gtggggtcgt cgttcacgta ggtgtcgcag atgaacacgg
     7201 tgcgcttggg cagcatcagg atgttcatcg cgtagtagtc cttcacgccg gggcgcaggc
     7261 cgatcacctg gtccacgtag tgcaggtgca ggcggtgcgt gccgaaggtg ccgcagatca
     7321 tcccgtcggc ctcgcccttg tgcatcatca tcgcgccgat cagcgtgtgg cggcggcgca
     7381 tctcgatctt ggcgtactgc atcgacacgc cgcggcgctc ggtgaggcga tggtattcgg
     7441 tccagtagtc ccggtaacgc gcgtcccact ccgggttcac gacgtcgaaa tgctcgccgg
     7501 cgttgaggcg caggccgaac cgctcgatgc gcttctcgag caccgtcggg cggcccacca
     7561 ggacgggacg cgcgagcttc tcgtccacca ccacctgcac cgcgcgcagc acccgctcgt
     7621 cctcgccctc ggcgtacacg atccgcatcg gggacgccct cgccgcggtg aagatgggcc
     7681 gcatgatgtg gcccgagtgg tagacgaact cgttcaggcg ctgcgcgtag gcctcgaagt
     7741 cgcggatggg gctcgtcgcg acgccgctgt ccatcgccgc ctttgcgacc gccggggcga
     7801 tcttcacgat gaggcgcggg tcgaagggct tcgggatcag gtactcgcgc ccgaagctca
     7861 cctccttctg ctggtaggcc atggcgacga cgtcggactg ctcggccatc gcgaggtcgg
     7921 cgatcgcgtt cacggcggcg agcttcatcg gctcgttgat cgtcgtcgcg cccacgtcca
     7981 gcgcgccgcg gaaaatgaac gggaagcaca gcacgttgtt cacctggttc gggtagtccg
     8041 aacggccggt ggcgatgatc gcgtcgtcgc gcaccgcctt gacctcggcc ggcaggatct
     8101 cgggcacggg atttgccagg gcgaagacga ggggattggc cgccatgcgc
//
LOCUS       DASUEV010000072         8784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1614046, whole genome shotgun sequence.
ACCESSION   DASUEV010000072 DASUEV010000000
VERSION     DASUEV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614046"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFV55_02300"
     CDS             <1..581
                     /locus_tag="VFV55_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4943153.1"
                     /translation="WRAEKVREHCQKYIDLVSLTGSESKRPAELSGGMKQRVGIARAF
                     AIQPKMLLLDEPFGALDALTRGAIQEELTAICAGTQQTVFMITHDVDEAILLADKIML
                     MTNGPKARVAEIVVNTLPRVRSRRDVHHHPHYYRIRNHLVDFLVHRSKLLQEGRAEDA
                     ANEEGPRVVMPGLAEGDDTLVAPHPALLKQVK"
     gene            661..1104
                     /gene="cynS"
                     /locus_tag="VFV55_02305"
     CDS             661..1104
                     /gene="cynS"
                     /locus_tag="VFV55_02305"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX4943154.1"
                     /translation="MTREDVTDLIYSAKVQKDIKWADVARKVGESKEWVTAACLGQMA
                     LTKAQAAKVGKIFGLPEAAVRLLQVPPYKGSLPTAVPTDPLIYRWYEIVNVYGTTIKE
                     LIHEEFGDGIMSAIDFSLDITREPDPKGDRVHVVLSGKFLPYKTY"
     gene            complement(1151..2083)
                     /locus_tag="VFV55_02310"
     CDS             complement(1151..2083)
                     /locus_tag="VFV55_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEX4943155.1"
                     /translation="MTTITEVAAAVIERPDGAFLLACRPEGKPYPGYWEFPGGKIEPG
                     EDARAALSRELKEELGIAVREATPWITRVYAYTHATVRLRFFRVTTWDGEPQPLEDQA
                     IAWQWPGKADVAPMLPANAPVLAALALPSVMVVSNAIETGFDAWALKLSAQVIEEKLL
                     VQIREKGLEHQRVQHLLSRALTRGTPFGAIVVVNSDCGTFPQADGVHLTSRALMAATA
                     RPAGRVVGASCHDASEIAQAAKLGLDYVVVGPVMPTASHPGAVLLGWEGFAALVSDCP
                     LPAYAIGGLARADLAAAKRHGAHGVALRSAAFNA"
     gene            complement(2080..2955)
                     /locus_tag="VFV55_02315"
     CDS             complement(2080..2955)
                     /locus_tag="VFV55_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4943156.1"
                     /translation="MSESLDRFLGRVDTLIDRLERILPEGARPVDWTAGTAFRWRKRN
                     ARGFLDPVRHPHAIAFADLKDIDDQKKRLRENTAQFVAGRPANNVLLTGARGTGKSSL
                     VKAALNDFARKGLRLIEVDKADLVDLPDIVDLVADRRERFIVFCDDLSFEASDPGYKS
                     LKVALDGTIAGSADNVLIYATSNRRHLMPEYMGENLEARHVGGEIHPGETTEEKISLS
                     ERFGLWLSFYPFDQDQYLDIAAHWVGALGGKADEGMDPAALLWALERGSRSGRVAWQF
                     AKDYVGRATGARSRK"
     gene            complement(2952..3611)
                     /locus_tag="VFV55_02320"
     CDS             complement(2952..3611)
                     /locus_tag="VFV55_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4943157.1"
                     /translation="MTATVTPQLETYYAKRAREYERIYDKVERQEELAWLRERIPQLF
                     AGRRVLEIACGTGYWTQFIAKTAAHVTACDINEPVLEIAREKPIAKGRATFVKADAFA
                     PAGVCHGCDAAFAGFWWSHVRKSDLTRWLAEMAKALPPGALLAMLDNRYVEGSSTPLS
                     RRDAEGNTYQVRPLLSGETHEVLKNFPTAAELAEAVRPFAAEAHLEETLYYWLLVFRL
                     K"
     gene            complement(3630..4859)
                     /gene="argJ"
                     /locus_tag="VFV55_02325"
     CDS             complement(3630..4859)
                     /gene="argJ"
                     /locus_tag="VFV55_02325"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX4943158.1"
                     /translation="MAVNLLSPKAESLLSIAGLRLGVAEAGIRKKDRRDLLVVEIADG
                     SRVAGVFTQNRYCAAPVTVCRERLAGKRAIRALVVNTGIANAGTGETGLANARATCAA
                     VARALGVDAGSVLPFSTGVIMEHLPVERITAGIPAAVANLRVDGWADAAAAIMTTDTV
                     PKAVSRQVVIDGCTITVTGIAKGAGMINPDMATMLGFIATDAAVDHGTLGDMVRYVAD
                     LSFNAVTVDGDTSTNDSFVLVATGRAGNPEIVGEGTAEFTRLAECVLEVAQILSQAIV
                     RDGEGATKFMTIVVEGGATREECQRVGKAIAHSPLVKTAFFASDPNLGRILCAVGNGG
                     IADLDPRNVTLHLGDVLVAEKGGRAQAYTEEQGKAVMAQPEITIRVGLGRGVECATVW
                     TCDFSYDYVKINADYRS"
     gene            complement(4940..7693)
                     /gene="secA"
                     /locus_tag="VFV55_02330"
     CDS             complement(4940..7693)
                     /gene="secA"
                     /locus_tag="VFV55_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX4943159.1"
                     /translation="MFANVLKAIFGSRNDRLLKLYRKSVESINALEPAIEKLSDEALV
                     AKTPELRAKVAAGTPLDDVLPEAFAVVREASRRVLGMRHFDVQLVGGLALHQGKIAEM
                     RTGEGKTLVATLPSYLNALSGKGVHVVTVNDYLARRDAEWMGRIHRFLGLSVGVVVPQ
                     MEHAEKQAAYLCDITYGTNNEFGFDYLRDNMATQVAERFQRGLNYAIVDEVDSILIDE
                     ARTPLIISGQAEDSTDLYRRINALIPSLSKQDDEKAEGDYWVDLKQHSVTLSEQGHEH
                     AEELMTREGLLPEGASLYDPANIMLMHHLYAGVRAHALYHRDQHYVVQEDEVVIVDEF
                     TGRMMAGRRWSDGLHQAVEAKEGVAIKNESQTLASITFQNYFRLYKKLSGMTGTADTE
                     AFEFSQIYNLETVLIPTHRPMIRKDTNDQVFITAKEKYQAISNAVKDCHERSQPVLLG
                     TTSIENSELISAMLDKEGLPHQVLNAKQHAREAEIVAQAGRPKMVTIATNMAGRGTDI
                     VLGGNPDPEIQRVKADEALTDAEKESRIAAIRENWQKLHNEVVAAGGLHIIGSERHES
                     RRIDNQLRGRAARQGDPGSSCFFLSFEDPLLKIFAGERLQGIMRSLKIPEGEAIEAKM
                     VTRSIENAQRKVEARNFDIRKQLLEYDDVANDQRKVIYTQRNELLETSDITPTIAAIR
                     ADVLAGVVREHVPPESVEEQWDLPALERHLESDFFMAAPVKQWVEKDETLDDEKIAAK
                     VVAQAAADYEAKFAPIEKEVLHQYERSVMLQSIDTHGREHLSALDYLRQGIHLRGYAQ
                     KQPKQEYKREAFELFGAMLDSIKRQVTQHLMAVQIRSQAEVEEAERKAEEAAQAVKNV
                     QYVHADYDEALAQAPEEGAAVAAADPPPAQPFVRGSQKVGRNDPCPCGSGKKYKNCHG
                     KLA"
     gene            complement(7816..8772)
                     /locus_tag="VFV55_02335"
     CDS             complement(7816..8772)
                     /locus_tag="VFV55_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX4943160.1"
                     /translation="MNIILVSDSLAKSRNVSLSQSQVVLVALGILMSGFFLAMATYVV
                     TMKFATDLRNPYLRSLVTALHEEDLKRSESEMRENINSLAVKVGEIHARMLRLDAFGE
                     RLAKAAGIKPTEFRFDEQPGQGGAAPSPAFSRDLTLPEFRQKLDEISRILDDRSDKMG
                     VLDSVFMDDRLARKAIPTTLPVHQGFYSSNYGYRIDPISGRSSFHTGVDIIASPGTPV
                     MAAAGGVVSAAEFHPEYGNIVDIDHDNGLTSRYAHLLKSVVRVGDVVLKGQLIAQVGN
                     TGRTTGPHLHFEVREKGVPLNPNKFLAMDRKEGQIPIALRNR"
BASE COUNT         1368 a         2946 c         2948 g         1522 t
ORIGIN      
        1 actggcgtgc cgaaaaggtg cgcgagcatt gccagaagta catcgatctc gtgagcctga
       61 ccggctccga aagcaagcgt ccggcggagc tctccggcgg catgaagcag cgggtgggca
      121 tcgcgcgcgc cttcgcgatc cagcccaaga tgctgcttct cgacgagccg ttcggggcgc
      181 tcgacgccct cacgcgcggc gccatccagg aggagctcac cgcgatctgc gccgggacgc
      241 agcagaccgt cttcatgatc acccacgacg tggacgaggc gatcctgctc gccgacaaga
      301 tcatgctgat gaccaacggg ccgaaggcgc gagttgccga gatcgtcgtc aacacgctcc
      361 cacgcgtccg ctcgcgccgc gatgtccacc accatccgca ctactaccgc atccgcaacc
      421 acctcgtcga cttcctcgtc caccgctcga agctcctcca ggaaggacgc gccgaggacg
      481 cggccaacga ggaaggtccc cgggtggtga tgccgggcct cgcggaagga gacgataccc
      541 tcgtggcgcc gcacccggcg ctgctcaagc aggtcaagtg aaaccccaat gcccggagga
      601 aacatgaaga gttcgataaa ggaaaacgcc gcgctggccg ccgtggccgg catgaagccc
      661 atgacccgtg aggatgtgac cgacctcatc tactcggcca aggtccagaa ggacatcaag
      721 tgggccgacg tcgccaggaa ggttggcgag tcgaaggagt gggtcaccgc cgcctgcctg
      781 ggacagatgg cgctcaccaa ggcgcaggcc gcgaaggtcg ggaagatctt cgggctgccg
      841 gaagcggcgg tcaggctgct gcaggtgccc ccttacaagg gatcgcttcc gaccgcggtg
      901 ccgaccgatc cgctgatcta ccgctggtac gagatcgtca acgtctacgg caccacgatc
      961 aaggaactca tccacgagga attcggcgac ggcatcatga gtgccatcga tttctccctg
     1021 gacatcacgc gcgagcccga tcccaagggc gatcgcgtgc acgtggtgct ctccggcaag
     1081 ttcctgcctt acaagaccta ctgactggaa aggggacgct tcccttttct tcccgggtcc
     1141 gcgtgccccg tcaggcgttg aacgccgccg agcgtagcgc gacgccgtgc gcgccatggc
     1201 gtttcgccgc agcgaggtcg gcgcgcgcaa gaccgccgat cgcgtaggcc ggcagcgggc
     1261 agtcgctcac cagcgcggcg aagccctccc agccgagaag cacggctccc gggtgcgatg
     1321 cggtgggcat caccggccct acgaccacgt aatcgaggcc cagcttcgcg gcctgcgcaa
     1381 tttcggatgc gtcatggcag gaggcgccga cgacacgccc cgccggccgc gccgtcgcag
     1441 ccatcagggc gcgcgacgtg aggtgcacgc cgtcggcctg cggaaaagtg ccgcaatcgc
     1501 tgttcaccac gacgatcgcg ccgaaaggcg tgccgcgggt gagcgcgcgc gaaagcaggt
     1561 gctggacgcg ctggtgctcg aggccctttt cgcggatctg cacgagcagt ttctcctcga
     1621 tcacctgcgc ggagagcttg agcgcccagg cgtcgaaccc cgtctcgatg gcgttggaaa
     1681 ccaccatcac cgagggtagc gcaagtgccg cgagcaccgg cgcattggcc ggaagcatcg
     1741 gcgcgacgtc cgccttcccc ggccactgcc aggcgatggc ctggtcctcg agcggttgcg
     1801 gctctccgtc ccacgtggtt acgcggaaga agcgcaggcg cacggtcgcg tgcgtgtagg
     1861 catagacccg cgtgatccag ggcgtcgcct cgcgcaccgc gatgcccagc tcctccttca
     1921 actcgcgcga gagcgccgcg cgggcatctt cgcccggctc gatcttgccg cccgggaatt
     1981 cccagtagcc gggatagggc ttgccctcgg gccggcaggc gaggaggaac gcgccgtcgg
     2041 gccgctcgat gaccgcggcg gcgacttccg tgatcgtcgt cacttgcggg aacgcgcgcc
     2101 ggtcgcgcga cccacgtagt ccttggcgaa ctgccaggcg acgcggccgc tcctcgatcc
     2161 gcgctccagg gcccacagga gcgccgcggg atccatgccc tcgtccgcct tgccgccgag
     2221 cgccccgacc caatgcgccg cgatgtcgag gtactggtcc tggtcgaaag gatagaagga
     2281 gagccacagc ccgaaccgct ccgagagcga gatcttctcc tccgtggtct cgcccggatg
     2341 gatctcgccc ccgacgtgcc tcgcctcgag gttctcgccc atgtattcgg gcatgaggtg
     2401 gcggcggttg gaggtcgcgt agatgaggac gttgtcggcg gacccggcga tggtgccgtc
     2461 gagcgccacc ttgagcgact tgtaccccgg gtcggaggcc tcgaaggaca ggtcgtcgca
     2521 gaagacgatg aaccgctcgc ggcgatcggc caccaggtcc acgatgtccg gcaggtccac
     2581 gaggtcggcc ttgtccacct cgatgaggcg caggcccttc ctcgcgaagt cgttcagcgc
     2641 cgccttcacc agcgagctct ttcccgttcc gcgcgcgccg gtgagaagca cgttgttggc
     2701 cggccggccc gcgacgaatt gcgccgtgtt ctcgcgcagg cgcttcttct ggtcgtcgat
     2761 atccttcagg tcggcgaagg cgatggcgtg cgggtggcgc accgggtcga ggaagccgcg
     2821 ggcgtttcgc ttgcgccagc ggaaagccgt tccggccgtc cagtcgacgg gccgcgcgcc
     2881 ctcggggagg atgcgctcga gccggtcgat gagcgtgtcg acgcgaccga gaaaacgatc
     2941 cagggattcg ctcatttgag gcggaagacc aggagccagt agtacagcgt ttcctccagg
     3001 tgcgcttcgg cggcgaaggg ccgcaccgcc tcggcaagtt cggcggccgt cgggaagttc
     3061 ttgagcacct cgtgcgtctc gccggatagc agcgggcgga cctggtaggt gttgccctcc
     3121 gcgtcgcggc gcgacagggg cgtggagctg ccctcgacgt aacggttgtc gagcatcgcg
     3181 agaagcgcgc ccggcggaag cgccttcgcc atttccgcga gccagcgcgt gaggtcggac
     3241 ttcctcacgt gcgaccacca gaacccggcg aaggccgcgt cgcatccgtg gcacacgccg
     3301 gcgggggcga acgcatcggc cttcacgaag gtggcacgcc ccttcgcgat gggcttctcg
     3361 cgcgcgatct cgagcaccgg ttcgttgatg tcgcacgcgg tcacgtgcgc cgcggtcttc
     3421 gcgatgaact gcgtccagta gcccgtcccg caggcgatct cgaggacacg ccggcccgcg
     3481 aagagctgcg gaatgcgctc gcgcagccac gcgagttcct cctggcgctc gaccttgtcg
     3541 tagatgcgct cgtactcccg cgcgcgcttc gcgtagtagg tttcgagttg cggggtcacg
     3601 gtggcggtca tcgcttcacg cacgcgcggc tagcttcggt agtccgcatt gatcttcacg
     3661 tagtcgtagg agaaatcgca agtccagacc gtggcacact ccacgccccg gcccaggccg
     3721 acacgaatgg tgatctcggg ttgcgccatc accgccttgc cttgctcctc ggtgtaggcc
     3781 tgcgcgcgcc cgcccttttc cgcgaccagc acgtcaccca ggtgcagggt gacgttccgg
     3841 ggatccaggt cggcgatgcc cccgttgccc accgcgcaca ggatgcggcc caggttcggg
     3901 tcggaggcga agaacgccgt cttcaccagc ggcgaatggg cgattgcctt gccgacgcgc
     3961 tggcattcct cgcgcgtggc gccgccttcc acaacgatgg tcatgaactt ggtggcgcct
     4021 tctccgtccc tcacgatggc ctgcgagagg atctgcgcaa cttccagcac gcattccgcg
     4081 aggcgcgtga actccgccgt gccctcgccg acgatctcgg gattgccggc gcggcccgtg
     4141 gcgacgagca cgaaggagtc gttggtcgag gtatccccgt cgaccgtcac cgcgttgaac
     4201 gagaggtccg ccacgtagcg caccatgtcc ccgagcgtgc cgtggtccac ggcagcgtcg
     4261 gtggcgatga agcccagcat cgtcgccatg tcggggttga tcatgcccgc gcccttggcg
     4321 atgccggtga cggtgatggt gcagccgtcg atcacgacct gccgcgacac cgccttgggg
     4381 accgtgtcgg tggtcatgat ggcggctgcc gcgtccgccc agccatcgac gcgcaggttc
     4441 gcgaccgccg cgggaatgcc ggcggtgatt cgctcgacgg gaaggtgctc catgatcacg
     4501 ccggtggaga aaggcaggac cgaacccgcg tccacgccga gagccctggc cacggcggcg
     4561 caggtcgcgc gcgcgttcgc aaggccggtc tcgcccgtgc ccgcgttggc aattccggtg
     4621 ttgacgacga gggcgcgaat ggcacgcttg cccgcaaggc gctcgcggca aaccgtcacc
     4681 ggggcggcgc aatagcgatt ctgcgtgaac acgcccgcca cccggctgcc atccgcgatc
     4741 tcgaccacga gaaggtcgcg acggtccttc ttgcggatgc cggcttcggc cacgccaagg
     4801 cgcaggccgg cgatggaaag aagcgattcc gccttgggcg aaaggagatt gacggccata
     4861 ggccccggat tatcgcgcga aagcgggcgg cgcgttcagc gcgccccggg tgccacgcga
     4921 cggggcgtca acccggccct caggcgagct tgccgtggca gttcttgtat ttcttgccgg
     4981 agccgcaggg gcatgggtcg ttgcgaccga ccttctggct gccgcgaacg aaaggctggg
     5041 ccggcggcgg atcggcagcg gccacggcgg cgccctcctc cggggcctgg gcgagcgctt
     5101 cgtcgtagtc cgcatggacg tactgcacgt tcttcaccgc ctgcgccgcc tcctcggcct
     5161 tgcgctcggc ttcctccacc tccgcctgcg agcggatctg gaccgccatc aggtgctgcg
     5221 tgacctgccg cttgatcgag tcgagcatcg cgccgaagag ctcgaaggcc tcgcgcttgt
     5281 actcctgctt gggctgcttc tgcgcgtagc cgcgcaggtg gatgccctgg cgcaggtagt
     5341 ccagcgccga caggtgctcc cgcccgtgcg tgtcgatcga ctgcagcatc accgagcgtt
     5401 cgtactggtg cagcacttcc ttctcgatcg gcgcgaactt ggcctcgtaa tccgcggcgg
     5461 cctgcgcgac cactttcgcc gcgatcttct cgtcgtcgag cgtctcgtcc ttttccaccc
     5521 actgcttcac cggcgcggcc atgaaaaagt cgctctcgag gtggcgctcg agggcgggaa
     5581 ggtcccactg ctcctcgacg ctttccggag gcacgtgctc gcgaaccacc ccggccagca
     5641 cgtcggcgcg gatggcggcg atagtcgggg tgatgtccga ggtctccagc agctcgttcc
     5701 tctgcgtgta gatgaccttg cgctggtcgt tcgcgacgtc gtcgtactcc agcaactgct
     5761 tgcggatgtc gaagttgcgg gcctcgacct tgcgctgggc attctcgatc gatcgcgtga
     5821 ccatcttcgc ctcgatggcc tcgccctccg ggatcttgag cgagcgcatg atcccctgca
     5881 ggcgctcgcc ggcgaagatc ttgagcagcg gatcctcgaa cgacaggaag aagcacgagg
     5941 aaccggggtc gccctggcgc gcggcgcggc cgcgcagctg gttgtcgatg cggcgcgact
     6001 cgtggcgctc ggagccgatg atgtgcagcc cgccggcggc gaccacctcg ttgtgcagct
     6061 tctgccagtt ttcgcggatt gcggcgatgc ggctttcctt ctccgcatcg gtgagtgcct
     6121 cgtcggcctt caccctctgg atctcggggt cggggttgcc gcccagcacg atgtcggtgc
     6181 cgcgcccggc catgttggtg gcgatggtga ccatcttcgg ccggcccgcc tgggccacga
     6241 tctccgcctc gcgcgcgtgc tgcttggcgt tgagcacctg gtgcgggagc ccctccttgt
     6301 cgagcatcgc cgagatgagc tcggagttct cgatcgaggt ggtgccgagc agaaccggct
     6361 ggctgcgctc gtggcaatcc ttcaccgcat tgctgatggc ctggtacttc tccttcgccg
     6421 tgatgaacac ctggtcgttc gtgtccttgc ggatcatcgg ccggtgcgtg ggaatcagca
     6481 ccgtctcgag gttgtagatc tgcgagaact cgaaggcctc ggtgtcggcc gtgccggtca
     6541 tcccggagag cttcttgtag aggcggaaat agttctggaa ggtgatcgag gcgagcgtct
     6601 gcgactcgtt cttgatcgcc acgccctcct tggcctccac cgcctggtgc aggccgtcgg
     6661 accagcgccg gcccgccatc atgcgccccg tgaactcgtc gacgatcacc acctcgtctt
     6721 cctgcaccac gtagtgctgg tcgcggtgat aaagcgcgtg cgcccgcacg ccggcgtaga
     6781 ggtggtgcat cagcatgatg ttggccgggt cgtagaggct cgccccctcg gggagcagcc
     6841 cttccctcgt catcagctcc tcggcgtgct cgtggccctg ttcggacagg gtcacgctgt
     6901 gctgcttcag gtccacccag tagtcgccct cggccttctc gtcgtcctgc ttcgacagcg
     6961 acgggatgag cgcgttgatg cgccggtaca ggtccgtgga atcctcggcc tggccggaga
     7021 tgatgagcgg cgtcctcgcc tcgtcgatga ggatcgagtc cacctcgtcg acgatcgcgt
     7081 agttgaggcc gcgctggaat cgctccgcca cctgcgtggc catgttgtcg cgcaggtagt
     7141 cgaagccgaa ctcgttgttg gtgccgtagg tgatgtcgca caggtaggca gcctgcttct
     7201 ccgcatgctc catctgcggc accaccacgc ccacgctgag gccgaggaaa cggtggatgc
     7261 gccccatcca ttccgcgtcg cgccgggcga ggtagtcgtt cacggtgacc acgtggacgc
     7321 ccttgccgga gagggcgttc aggtacgacg gcagcgtcgc cacgagcgtc ttgccctcgc
     7381 cggtgcgcat ctcggcgatc ttgccctggt ggagtgccag cccaccgacg agctgcacgt
     7441 cgaagtggcg catgcccagc acgcgcctgg aggcttcccg cacgacggcg aaggcctcgg
     7501 gtaggacgtc gtcgagcggc gtgcccgcgg cgaccttcgc ccgaagctcc ggcgtcttgg
     7561 ccacgagggc ctcgtcggaa agcttctcga tggcgggctc gagggcgttg atggactcga
     7621 ccgacttccg gtacagcttc aggaggcggt cgttgcggct gccgaaaatg gccttgagga
     7681 cgtttgcgaa cacgggagcg ggcgcttggg cgggggacgg atgcgaaacc ggccccaacg
     7741 gggccggaga gagccgaaat tataaggtca aatggggccg ggctgccgga atgcaactcc
     7801 ggccggtccc ggcggctacc tgttgcgcaa ggcgatcggg atctgccctt ctttcctgtc
     7861 catcgcgagg aacttgttcg gattgagcgg cacgcccttt tcccggacct cgaagtgcag
     7921 gtgcggcccg gtggtgcgcc ccgtgttgcc gacctgcgcg atgagctggc ctttcagcac
     7981 cacgtctccc accctgacca cgctcttcag caggtgtgcg tagcgcgagg tcaggccatt
     8041 gtcgtggtcg atgtcgacga tgttgccgta ctccggatgg aactccgccg ccgacacgac
     8101 gcccccggcc gccgccatca ccggcgtgcc cggcgaggcg atgatgtcca ccccggtatg
     8161 gaagctcgag cggccgctga tcggatcgat gcgatagccg tagttggagg agtagaagcc
     8221 ctggtggacc gggagggtcg tggggatcgc cttgcgcgcc aggcggtcgt ccatgaacac
     8281 cgagtcgagg acccccatct tgtcgctccg gtcgtcgagg atgcgcgaga tctcgtcgag
     8341 cttctgcctg aattccggaa gcgtcaggtc gcgggaaaag gccggcgagg gggcggcgcc
     8401 gccctggccg ggctgctcgt cgaagcggaa ttcggtgggc ttgatgccgg cggccttggc
     8461 caggcgctcg ccgaacgcgt ccaggcgaag catccgggca tggatctctc ccaccttcac
     8521 cgcgagggaa ttgatgttct ccctcatttc cgactcgctg cgcttgaggt cctcctcgtg
     8581 cagcgcggtg acgagcgaac gcaagtaggg gttgcgcaga tccgtcgcga acttcatcgt
     8641 gacgacatag gtcgccatcg ccaggaagaa acccgacatc aggatgccca gcgccaccag
     8701 gacaacctgg gactgggaca gcgagacatt gcggcttttt gccaggctgt ccgaaaccag
     8761 aataatgttc atccgtcact cccc
//
LOCUS       DASUEV010000073         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_594977, whole genome shotgun sequence.
ACCESSION   DASUEV010000073 DASUEV010000000
VERSION     DASUEV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594977"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFV55_02340"
     CDS             complement(<1..744)
                     /locus_tag="VFV55_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX4943161.1"
                     /translation="MNALTLYDKLWQSHLVHEEPDGTALIYIDRHLVHEVTSPQAFDG
                     LAIAGRKPWRIGSVVATADHNVPTRNRAGGIADPIARLQVETLDRNVAKFAVPVYYGM
                     SDARQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFAALAFGIGTSEVEHVLATQCL
                     LAKKAKSLRIRVEGALPRGVSAKDVVLAVIGRIGTAGGTGHAIEFAGSAIRSLTMEGR
                     MTVCNMAIEAGARAGMIAVDGTTISYLKGR"
     gene            complement(798..1955)
                     /gene="aroC"
                     /locus_tag="VFV55_02345"
     CDS             complement(798..1955)
                     /gene="aroC"
                     /locus_tag="VFV55_02345"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX4943162.1"
                     /translation="MSGNSLGTLFAVTTFGESHGPAIGCVVDGCPPGMPLSEADIQPE
                     LDRRKPGTSRHVTQRREEDLVEILSGVYEGRTTGTPIALLIRNEDQRSKDYAKIASSF
                     RPGHADYTYLQKYGIRDPRGGGRSSARLTAPAVAAGAIAKKWLRERYGIVVRGHLTRI
                     GPNEIPFESWDHVDGNPFFAANASIVPELEAFMDRLRKSGDSCGARLTVVASGAPPGW
                     GEPLYGRLDADIAQAMMGINAVKGVEIGAGFAAAGQLGTEHSDEMTPQGFLSNHAGGI
                     LGGISTGQDIVVSIAIKPTSSIRLARRTVDVAGNPATIETHGRHDPCVGIRATPIAEA
                     FLAIVLMDHALRHRAQNADVSCATPRIPAAAPSISGGGSGEVLEDPDPGEA"
     gene            2081..>3257
                     /locus_tag="VFV55_02350"
     CDS             2081..>3257
                     /locus_tag="VFV55_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX4943163.1"
                     /translation="MLDDIKTSALDYHRLPRPGKIEISPTKPLATQRDLALAYSPGVA
                     FACEAIQESPAEARNLTARGNLVGVVTNGTAVLGLGNIGPLAAKPVMEGKGVLFKKFA
                     GIDVFDLELNELDPDRLIEVIAALEPTFGGINLEDIKAPECFYIERKLRERLKIPVFH
                     DDQHGTAIIVGAAIVNGLRVVGKQIGDVKLAVSGAGAAALSCLDMLVALGMKIGNIWV
                     SDIAGVVYEGRTELMDENKARYAKKTDARTLGDIIGDADVFLGLSAGGVLKPDMVKRM
                     AANPLVFALANPVPEILPAEVKAVRDDAIIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTINEPMKLAAVNAIADLAMAEQSDVVAMAYQQKEVSFGREYLIPKPFDPRLIVKIA
                     "
BASE COUNT          540 a         1116 c         1079 g          522 t
ORIGIN      
        1 tcgccctttc aggtaggaaa tcgtggtgcc gtccacggcg atcatgcccg cgcgagcgcc
       61 ggcctcgatg gccatgttgc acacggtcat gcgtccttcc atcgtgagcg accggatggc
      121 cgagccggcg aactcgatcg cgtgcccggt gccgcccgcg gttccgatgc gcccgatcac
      181 ggccagcacc acgtccttgg ccgagacacc acggggcagc gcgccctcga cgcgaatgcg
      241 cagcgacttc gcctttttcg caagaaggca ctgcgtggcg agcacgtgct cgacctcgct
      301 ggtgccgata ccgaaggcga gtgcggcgaa cgccccgtgc gtactggtgt gcgaatcgcc
      361 gcagacgacc gtcatgcccg ggagcgtggc gccctgctcc gggccgatga cgtggacgat
      421 cccctgccgt gcatccgaca tgccgtaata gaccggcacg gcaaacttcg cgacgttgcg
      481 gtcgagggtc tcgacctgca gccgggcgat cggatcggcg atgccgccgg cgcggttgcg
      541 ggtcgggaca ttgtggtcgg cggtcgccac caccgagccg atgcgccacg gcttgcggcc
      601 ggcgatcgcg aggccatcga aggcctgcgg gctcgtcacc tcgtgcacga ggtgacggtc
      661 gatgtagatg agcgccgtcc cgtccggttc ctcgtggacg agatgggact gccaaagctt
      721 gtcgtagaga gtgagcgcgt tcatggtctt ccgggcaagg ggaaattata acccgcgccc
      781 gccacccctt cggaccctca cgcctccccg ggatcgggat cctcgaggac ttcgcccgat
      841 ccgccgccgg aaatcgaagg cgctgcagcc ggtatgcgcg gcgtggcgca cgacacatcc
      901 gcgttctgcg cccggtggcg aagcgcatgg tccatgagca cgatcgcgag gaatgcctcc
      961 gcgatcggcg tggcgcggat tccgacgcag ggatcgtgac gaccgtgcgt ctcgatggtc
     1021 gccggattgc ctgcaacgtc aacggtgcgc cgggcgaggc ggatgctcga ggtgggcttg
     1081 atcgcgatcg acaccacgat gtcctgaccc gtcgaaatgc ccccgaggat cccgcccgcg
     1141 tggttcgaaa gaaagccctg cggcgtcatt tcgtcggaat gttccgtgcc cagctgtccc
     1201 gccgcggcga atcccgcgcc gatctcgacg cccttcacgg cgttgatgcc catcatcgcc
     1261 tgggcgatgt cggcgtcgag gcggccatag agcggttcgc cccagcccgg cggagcgccg
     1321 gacgccacga ccgtgagccg cgcgccgcag gaatcgcccg acttgcgcaa ccggtccatg
     1381 aacgcctcca gctccgggac gatcgacgcg ttggccgcga agaacggatt gccatcgacg
     1441 tgatcccacg actcgaacgg gatttcattc ggcccgatcc gggtcaggtg cccgcgcacc
     1501 acgatgccgt agcgctcgcg cagccacttc ttcgcgatcg cgcccgcagc gacggccggc
     1561 gcggtgagtc gcgccgatga gcgcccaccg ccgcgcggat cgcggatgcc gtatttctgg
     1621 aggtacgtgt agtcggcatg gccgggacgg aagctcgagg cgatcttcgc gtagtccttg
     1681 ctgcgctggt cctcgttgcg gatgaggagc gcgatcggtg ttcccgtcgt gcgtccctcg
     1741 tagacaccgc tgaggatctc gacgagatcc tcctcgcgcc gctgcgtcac gtgccgcgac
     1801 gttcccggct tgcgccggtc gagttcgggc tggatatcgg cttcggagag cggcattccc
     1861 ggcgggcagc catccacgac gcagccgatg gccgggccgt gggactcgcc gaaagtggtg
     1921 acggcaaaga gggttccgag gctgttgccg gacatgatgc gcagggggaa ggcgttcaac
     1981 cgagtttagc atgcgcaacg tctcgccctt tcccgcgcgc cgtgtcggta ccccatgcta
     2041 gacttcacgc aaacttcaag ccgctcaagg gtttagcacc atgctcgacg acatcaagac
     2101 aagcgcactc gactatcacc gcctgccgcg gcccggcaag atcgagatct cccccaccaa
     2161 gccgcttgcc acgcagcgcg acctcgcgct cgcctactcg ccgggggtgg ccttcgcctg
     2221 cgaggccatc caggaatcgc ccgccgaggc gcgcaacctc accgcccgcg gcaacctcgt
     2281 cggcgtcgtc accaacggca cggccgtgct cggcctgggc aacatcgggc cgctggccgc
     2341 gaagccggtg atggagggca agggagtcct gttcaagaaa ttcgccggca tcgacgtctt
     2401 cgacctcgag ctgaacgagc tcgacccgga ccggctgatc gaggtcatcg cggcactcga
     2461 gcccacgttc gggggcatca acctcgagga catcaaggcc cccgagtgct tctacatcga
     2521 gaggaagctg cgcgagcgcc tgaagatccc cgtgttccac gacgaccagc acggcacggc
     2581 gatcatcgtg ggcgcggcga tcgtcaacgg gcttcgcgtg gtgggcaagc agatcggcga
     2641 cgtgaagctg gccgtgtcgg gagcgggtgc ggccgccctg tcctgcctcg acatgctcgt
     2701 ggccctcggc atgaagatcg gcaacatctg ggtctccgac atcgcgggcg tggtctacga
     2761 gggccgcacg gaactcatgg acgagaacaa ggcgcgctac gcgaagaaga ccgacgcgcg
     2821 cacgctgggc gacatcatcg gggacgcgga cgtgttcctc ggcctttccg cgggcggcgt
     2881 cctgaagccc gacatggtca agcgcatggc ggccaatccc ctcgtcttcg ccctcgcaaa
     2941 ccccgtgccc gagatcctgc ccgccgaggt caaggcggtg cgcgacgacg cgatcatcgc
     3001 caccggccgt tcggactacc cgaaccaggt gaacaatgtc ctgtgcttcc cgttcatctt
     3061 ccgcggcgcg ctggacgtgg gcgcgacgac gatcaacgag ccgatgaagc tcgccgccgt
     3121 gaacgcgatc gccgacctcg caatggccga gcagtccgat gtcgtcgcca tggcctacca
     3181 gcagaaggag gtgagcttcg ggcgcgagta cctgatcccg aagcccttcg acccgcgcct
     3241 catcgtgaag atcgccc
//
LOCUS       DASUEV010000074         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_709340, whole genome shotgun sequence.
ACCESSION   DASUEV010000074 DASUEV010000000
VERSION     DASUEV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709340"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFV55_02355"
     CDS             <1..250
                     /locus_tag="VFV55_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX4943164.1"
                     /translation="PLNLVYVAHGDRMAELMPEDRKLFCSVDAAFIGQNVYLYCASAG
                     LSTVFRGAVDYPRLVRAMRLPEDQFVVFAQTVGYPRGT"
     gene            272..946
                     /locus_tag="VFV55_02360"
     CDS             272..946
                     /locus_tag="VFV55_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX4943165.1"
                     /translation="MSSTPRLTVADLDRLESLLSAEHLADSMTLDALQGLFCAVASAP
                     APIPPEQWLATALGEHTFASGDEENEITDLLTRFCNDTARDLAAGAGLDVISYPAEEG
                     EEELAVWCEGYLVGVGIADPDWFTAGDEESVAELLFPFMALSGRLKEHALESGEPWEE
                     GEREAELMAGARDAFYDTVEEAHQYWFETRINRVPQKRDGEKVGRNDPCPCGSGKKYK
                     ACHGAG"
     gene            complement(956..1321)
                     /locus_tag="VFV55_02365"
     CDS             complement(956..1321)
                     /locus_tag="VFV55_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX4943166.1"
                     /translation="MGRNILDEDHIHPAIRAKVAGNHADIVQEVQQAIAANAVVVVGM
                     AGNPFPKQARRELDRAGVAYRYLGYGSYLNDWRRRNALKMWTGWPTFPMVFVKGVLVG
                     GASDLAKLIASGELKRMLS"
     gene            1357..>2541
                     /locus_tag="VFV55_02370"
     CDS             1357..>2541
                     /locus_tag="VFV55_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4943167.1"
                     /translation="MNDIVFQPARKLARLLRARKLSAVEVMKAFIAQVERVNPGVNAI
                     VTFLPGEAIREAKKLDAKRARARGADAIGPLAGLPIAYKDMIATKGIRTTYGSPIYAD
                     NVPAEDSLLVERLKAAGAITLGKTNTPEFAAGSQTFNPVFGATLNPFDLTKTCGGSSG
                     GAAVAVACGMLPFADGSDLGGSLRNPGNFNNVVGFRPTPGRVPVYPATDAWATLAVLG
                     PIARTVDDAAFLLSAMAGPDDRSPIGITEPGSRFARPLARDFRKVRVAWSRDLGGLPV
                     DRRVTAVLERQRKVFESLGCVVEEAQPDLTGADEVFHVLRAVGYVQKYEGLLATDRAR
                     IKDTVVWNIEQGLAMDGPRVARAMALRSEIYRRMREFMDRYEFLCLPVNQVPPFPVTQ
                     PYV"
BASE COUNT          409 a          887 c          833 g          412 t
ORIGIN      
        1 gcccctgaac ctcgtgtacg tggcccatgg cgatcgcatg gccgagctga tgcccgagga
       61 ccggaagctc ttctgctccg tggacgccgc gttcatcggc cagaacgtgt atctctactg
      121 cgcctccgca ggcctttcca ccgtcttccg cggggcggtg gactatccaa ggctggtgcg
      181 cgcgatgcgc ctgcccgagg accagttcgt cgtcttcgcg cagacggtgg gatatcctcg
      241 ggggacctga attccgcccc tgcctttcgc catgtcctcc accccccgcc tcaccgtcgc
      301 cgacctcgac cgcctcgaat cccttctctc ggctgaacac ctcgcggatt cgatgacgct
      361 ggatgccctg cagggccttt tctgcgcggt ggccagcgcc cccgccccga tcccgccgga
      421 gcaatggctc gccaccgccc tgggcgaaca cacgttcgcg agcggagacg aggagaacga
      481 aatcaccgat ctcctcacgc gcttctgcaa cgacacggca agggacctgg ccgcgggagc
      541 cggcctcgac gtcatttcct atcccgcgga agaaggcgaa gaggaactcg cggtgtggtg
      601 cgagggctac ctcgtgggcg tggggatcgc ggacccggac tggttcacgg cgggcgacga
      661 ggagtcggtt gccgagctcc tctttccgtt catggcactc tccgggcgcc tcaaggaaca
      721 cgcgctggag tcgggcgagc cctgggagga aggcgagcgg gaagccgagc tcatggccgg
      781 ggcgcgcgac gcgttctacg acacggtcga ggaggcccac cagtactggt tcgaaacccg
      841 catcaaccgc gttccgcaga agcgcgacgg cgagaaggtg ggccgcaacg acccgtgccc
      901 gtgcggcagc gggaagaagt acaaggcctg ccacggggca ggctaggcgc caccgctacg
      961 aaagcatccg cttcaattcg ccgctggcga tgagcttcgc caggtcgctc gcgccgccca
     1021 cgagcacccc cttcacgaac accatcggaa aggtcggcca ccccgtccac atcttgagcg
     1081 cgttgcgccg ccgccagtcg ttcaggtagc tgccataccc gaggtagcga taggcaacgc
     1141 cggcacgatc gagttcccgg cgcgcctgct tcggaaacgg gtttcccgcc atgccgacca
     1201 ccaccacggc gttggccgcg attgcctgct gcacttcctg cacaatgtcg gcgtggttgc
     1261 ccgcaacctt cgcgcggatg gcggggtgga tgtggtcctc gtcgaggatg ttgcggccca
     1321 tgatccggtc cttttccctg ggttgacctt cgaaccatga acgatatcgt ctttcagcct
     1381 gcccgcaagc tcgcccgcct cctgcgtgcc cgaaagctct ccgccgtcga ggtgatgaag
     1441 gcgttcatcg cccaggtcga gcgcgtgaat cccggcgtga acgccatcgt caccttcctg
     1501 cccggcgaag cgatccggga ggcgaagaag ctcgatgcga agcgggcgcg cgccaggggc
     1561 gccgacgcca tcgggccgct cgcgggcctg ccgatcgcct acaaggacat gatcgcgacg
     1621 aagggcatac gcaccaccta cggctcgccg atctacgcgg acaacgttcc ggccgaggac
     1681 agcctcctcg tcgaacgcct gaaggccgcc ggcgccatca cgctgggcaa gaccaacacg
     1741 ccggagttcg cggccggcag ccagaccttc aatccggttt tcggggcaac gctcaacccg
     1801 ttcgacctta cgaagacttg cggcggctcc tccgggggcg cggccgttgc ggtggcctgc
     1861 ggcatgctgc cctttgccga cggctcggac ctgggcggaa gcctgcgcaa cccgggcaac
     1921 ttcaacaacg tggtgggttt ccggccgaca ccggggcgcg ttcccgtcta tcccgcgacc
     1981 gatgcctggg cgacgctggc agtgctcggg cccatcgccc gcacggtgga cgacgcggcg
     2041 ttcctgctct ccgcgatggc cggtcccgac gaccgctcgc cgatcggcat caccgagccg
     2101 ggcagccggt tcgccaggcc gctcgcgagg gatttccgga aggtgcgcgt cgcatggtca
     2161 agggatctcg gcggcctgcc ggtggacagg cgcgtgactg cggtgctcga gaggcagcga
     2221 aaggtgttcg agtcgctcgg ctgcgtcgtc gaggaggcgc agcccgacct caccggcgcc
     2281 gacgaagtct ttcacgtgct gcgcgccgtg ggatacgtgc agaaatacga aggcctcctc
     2341 gccacggatc gcgcgcggat caaggacacg gtcgtctgga acatcgagca gggcctggcg
     2401 atggacggcc cccgcgtggc gcgggcgatg gcgctgcgct cggagatcta tcgccgcatg
     2461 cgcgagttca tggaccgcta cgagttcctc tgcctgcccg tgaatcaggt gccgcccttc
     2521 cccgtgacgc aaccctacgt c
//
LOCUS       DASUEV010000075         6261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_709791, whole genome shotgun sequence.
ACCESSION   DASUEV010000075 DASUEV010000000
VERSION     DASUEV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709791"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..977)
                     /locus_tag="VFV55_02375"
     CDS             complement(126..977)
                     /locus_tag="VFV55_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4943168.1"
                     /translation="MPPPASPVGRDRPSHPGIASVEFTSLAVRAVRKVAAEEILSRFR
                     TVTAQRKDDGSLVTEADLAAQVALTDVLGRIEPVDVLGEEMAEAEQRRIWEGGGRFWC
                     VDPLDGTANFSAGVPYFAVSVALMQGDRPLFGVVVDPIADEAYFAVRGAGAWRDGFAL
                     RRPAGEIGLESAIAEIDLRRSLAHVRHEIKHHPPFARRLSCGSSALSWCHLAAGRTDI
                     LLHAGQKVWDYAAGALIAEEAGMRIATLAEEDFWSGPAWERSVIAARTPELFAAWRDW
                     VRARLAL"
     gene            1049..1783
                     /locus_tag="VFV55_02380"
     CDS             1049..1783
                     /locus_tag="VFV55_02380"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX4943169.1"
                     /translation="MASPRIFCDLRLGPGAQFSLPQDAANHVGRALRLRAGDALVVFD
                     GRGGEFEAVIQRIDRDRVDVKTGAFSDADRESPIEVGLVQGLPEADKMDWTLQKSVEL
                     GVAWVQPVVCERSVVRLSGDRAARRESHWQRVVIAACEQCGRNRLPELRPTLAFRDWA
                     AQPSTARRWMLAPGADESLAGQPAPAGPVELLVGPEGGLSERELDIAAMIGFASLSLG
                     PRVLRTETAPLAALSAMQALWGDFRR"
     gene            1813..3141
                     /gene="argA"
                     /locus_tag="VFV55_02385"
     CDS             1813..3141
                     /gene="argA"
                     /locus_tag="VFV55_02385"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX4943170.1"
                     /translation="MPKTPAIKTEAFVKWFRSATPYIHQWGGATFVIAFGGEVLADGE
                     FQQLTHDINVLMSLEVRVVLVHGTRPQVEEQMRQHGVESRYVGNRRVTDARALSCVKE
                     ANGIVRVEMEALLSMELANSPMWGADIRVSSGNFVTAKPVGIVEGVDFGHTGEVRKID
                     AEGIRRRLDDHEVVLISPLGFSPTGDVFNCTLEDVATSTAIALRADKLIFLTETAGAL
                     DAKGRLISELTVKQGEELIAANGGLPEDVRIYLPCAIKACSQGVKRAHLISRHVDGAL
                     LIELFTHHGIGTMVVPESPEVIRDATDADIPGIVQIIEPLEQQGILVRRERDKLEREI
                     DRFFVIESDGNILGCAALYPFPGESTAELACLAVNPFYRDGGRGERLLAFAEVRARKA
                     GFKSLFVLSTRTTHWFIERGFGEADISRLPGSKQSLYNYERRSKIFTKEL"
     gene            3141..3410
                     /locus_tag="VFV55_02390"
     CDS             3141..3410
                     /locus_tag="VFV55_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX4943171.1"
                     /translation="MARMVNCIKLKKEAEGLDFPPYPGELGKKLWESVSKEAWKGWLE
                     QQKMLVNENRLNLADPRARKYLEEQMRRHFFGEGADAVQGYVPKG"
     gene            complement(3714..4424)
                     /gene="phoU"
                     /locus_tag="VFV55_02395"
     CDS             complement(3714..4424)
                     /gene="phoU"
                     /locus_tag="VFV55_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX4943172.1"
                     /translation="MNTQEHTSKRYDAELEAVRSRVLQMGGLVETQITAAIEALSTGN
                     ISLAEQTIADDHRVNALEVRIDEDCAHIIARRQPAAQDLRMIMTIIKMITDLERIGDE
                     AEKIARMTKLIYASERMLLPRFAEIKVAAGYALEMLKKSLDAFARVDIAAAAAVVKQD
                     RLLDEQFRGILRQLITFMMEDPRTISTSIEILFVAKALERIGDHAKNMSEYVVYLVKG
                     KDVRHITAEEFDRETSSH"
     gene            complement(4523..5188)
                     /gene="rpiA"
                     /locus_tag="VFV55_02400"
     CDS             complement(4523..5188)
                     /gene="rpiA"
                     /locus_tag="VFV55_02400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX4943173.1"
                     /translation="MDQDALKKLCAEAALAFVEDDMMVGVGTGSTVNHFIAALAARRS
                     RIAGTVSSSEASAKRLRAAGIPVADLNSTDGCDIYVDGADEVTESLAMIKGGGGALTR
                     EKIVAQASRRFVCIADASKLVAVLGKFPLPVEVIPMARSLVARQIVKLGGRPVLREGV
                     VTDNGNVILDVHGLAILDPAALESELNQLPGVVTNGLFARRPADVLLLGTAQGVRRID
                     RLR"
     gene            5231..>6261
                     /gene="ilvA"
                     /locus_tag="VFV55_02405"
     CDS             5231..>6261
                     /gene="ilvA"
                     /locus_tag="VFV55_02405"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX4943174.1"
                     /translation="MKTDYLRKILSARVYDVAIESPLELAPAISRRTGNHILLKREDK
                     QPVFSFKLRGAYNKMAHLKPEQLARGVICASAGNHAQGVALGAQKLGTTATIVMPVTT
                     PRIKVSAVAVRGAKVVLHGDSYHEAAQHAKAMAKKQGLTFVHPYDDPLVIAGQGTIGM
                     EILRQHQRPIDAIFVPVGGGGLIAGIASYVKRITPATRIIGVEPVDSNAMTASLKAGR
                     RVTLPHVNIFADGVAVRQVGRETFRLCRELVDEMVLVDTDEICAAIKDIFEDTRTVVE
                     PSGALALAGAKRWLARRRLKGRTVVAIACGANMNFDRLRFVAERAELGEHREAVLAVT
                     IPEKPGSFRA"
BASE COUNT         1010 a         2113 c         2125 g         1013 t
ORIGIN      
        1 ggaaaagggg tcaggttact tttcagcccg ccgtcttgcg acggcaatac gatagaaaag
       61 taacctgacc ccttttccgt tttgcctaat ttttgtgcaa agtaacctga cccctttgcc
      121 gatccctaca gcgcgagtct cgcgcgcacc caatcgcgcc acgccgcgaa gagctccggc
      181 gtgcgcgccg cgatcaccga gcgttcccac gccggccccg accagaagtc ttcctcggcg
      241 agcgtggcga tgcgcatgcc tgcctcttcc gcgatgaggg cgcccgcggc gtagtcccag
      301 accttctggc cggcgtgcag gaggatgtcg gtccggcccg ccgcgaggtg gcaccacgat
      361 agcgccgacg agccgcagct gagccgccgc gcgaacggcg gatggtgctt tatctcgtgc
      421 cggacgtggg cgaggctgcg ccgcaggtcg atttccgcga tggcgctctc gaggccgatc
      481 tcgccggcgg ggcggcgcag cgcgaagcca tcgcgccagg caccagcgcc gcgcaccgcg
      541 aaataggcct cgtcggcaat ggggtcgacc acgacgccga acagcggccg gtcgccctgc
      601 atcagcgcga ccgataccgc gaagtacggc accccggcgc tgaagtttgc cgtgccgtcg
      661 agcggatcga cgcaccagaa gcgcccaccg ccctcccaga ttcgccgctg ttcggcctcc
      721 gccatctcct cgccgagaac gtcgacgggc tcgatccggc cgagcacgtc ggtgagtgcc
      781 acctgcgccg cgaggtcggc ttccgtcacc aggctgccgt cgtccttgcg ctgcgcggtg
      841 accgtgcgaa agcgtgacag gatctcctcg gccgcgacct tgcgtacggc gcgcacggcc
      901 agcgaggtga attcgacgct tgcaatgccc gggtgcgatg gacgatcgcg gccgacaggc
      961 gaggcaggtg ggggcatgga gacgatgccg ggaggggttg cgatcggtat gttagcatcg
     1021 ggtcgattgc cgccttccgt gtttcaccat ggcttccccg cgcatcttct gcgatctccg
     1081 gctcggtccg ggcgcccagt tctcccttcc ccaggacgcc gccaatcacg tgggccgggc
     1141 gctgcgcctg cgcgccggcg acgcgcttgt cgtcttcgac ggccgcggcg gggaattcga
     1201 ggcggtcatc cagcgcatcg accgggaccg cgtcgacgtg aagacaggcg ccttcagcga
     1261 tgccgaccgc gagtccccga tcgaggtcgg gctcgtgcag ggcctgcccg aggcggacaa
     1321 gatggactgg accctgcaga aatcggtgga actcggcgtg gcgtgggtcc agcccgtggt
     1381 ctgcgagcgc agcgtcgtgc gcctgtccgg ggaccgtgcg gcgcgccgcg aatcccactg
     1441 gcagcgcgtg gtgatcgccg cctgcgagca atgcgggcgc aaccggctgc cggaactgcg
     1501 ccccacgctc gccttccgcg actgggcggc gcagccctcg acggcacggc gctggatgct
     1561 ggcgccgggc gcggacgaat ccctggcggg gcagccggcg ccggccgggc ccgtcgaact
     1621 gctcgtggga cccgagggtg gcctttccga gcgcgagctg gacatcgccg ccatgatcgg
     1681 gttcgcctcg ctctcgctgg gcccgcgcgt gctgcgcacc gagaccgctc cgctcgcggc
     1741 cctttccgcg atgcaggcgc tgtggggcga cttccgccgg tagaatccgt ccgcgatccc
     1801 cgaaacccgt cgatgcccaa gacccctgcg atcaagaccg aagccttcgt gaagtggttc
     1861 cggtcggcca cgccctacat ccaccaatgg ggcggggcga ccttcgtcat cgccttcggt
     1921 ggcgaggtgc tcgccgatgg cgagttccag cagctcaccc acgacatcaa cgtcctcatg
     1981 agcctggagg tgcgcgtggt gctggtgcac gggacccggc cgcaggtcga ggagcagatg
     2041 cgccagcacg gcgtggaatc gcgctacgtg ggcaaccgcc gcgtcaccga cgcgagggcg
     2101 ctttcgtgcg tgaaggaggc caacggaatc gtgcgcgttg aaatggaggc gctcctgtcc
     2161 atggagctcg ccaacagccc gatgtggggt gccgacatcc gcgtctcctc gggaaacttc
     2221 gtcaccgcga agccggtggg catcgtggag ggcgtggact tcggccacac cggcgaggtg
     2281 cgcaagatcg acgccgaggg catccgccgc cgcctggacg accacgaggt ggtgctcatc
     2341 tccccgctcg ggttctcgcc cacgggcgac gtgttcaact gcacgctcga ggacgtggcc
     2401 acctcgaccg cgatcgccct gcgcgcggac aagctcatct tcctgaccga gaccgccggc
     2461 gcgctcgacg cgaagggccg gctcatctcg gagctcacgg tgaagcaggg cgaagagctc
     2521 atcgccgcca atggcgggct tcccgaggac gtgcggatct acctgccctg cgccatcaag
     2581 gcgtgctcgc agggggtgaa gcgcgcgcac ctgatcagcc ggcacgtcga cggggcgctc
     2641 ctgatcgagc tcttcaccca ccacggcatc ggcacgatgg tggtgcccga atcgcccgag
     2701 gtgatccgcg acgcgacgga cgcggacata ccgggcatcg tgcagatcat cgagccgctg
     2761 gagcagcagg gcatcctggt gcgccgggag cgcgacaagc tcgagcgcga gatcgaccgg
     2821 ttcttcgtga tcgagagcga cggcaacatc ctcgggtgcg cggcgctcta tcccttcccg
     2881 ggggagtcga ccgcggagct cgcctgcctt gcggtgaacc cgttctaccg tgacggcggg
     2941 cgcggcgagc ggctcctcgc ctttgccgag gtgcgcgcgc ggaaggcggg gttcaagtcc
     3001 ctcttcgtgc tctccacccg cacgacgcac tggttcatcg agcgcggatt cggcgaggcc
     3061 gacatctcgc ggctgcccgg gagcaagcag tcgctctaca attacgaacg ccgctccaag
     3121 atcttcacga aggaactctg atggcacgca tggtgaactg catcaagctg aagaaggagg
     3181 ccgagggcct cgatttcccc ccgtatcccg gcgagctggg caagaagctc tgggagagcg
     3241 tgtccaagga agcgtggaag ggctggctcg agcagcagaa gatgctggtg aacgagaacc
     3301 ggctcaacct ggccgacccg cgggcgcgca agtatctcga ggagcagatg cgccggcact
     3361 tcttcggcga gggcgccgat gccgtgcagg ggtacgtgcc caaggggtga gggttgcgga
     3421 gtgatgcggg cgcaaacctt ggaacacgga tgaacacgga taaaggcgga tgaacacgga
     3481 tgaattccga tattcatcta ttcatcgaat gccaggagtg gtctgagcga accgtacact
     3541 tgaagccctc cattcctgca ccggaagccg cgatcaactc aagatttatc cgtgttcatc
     3601 cgcctttatc cgtgttcatc cgtgttccaa aggtttacgg ccgcatcact ccccgccgcc
     3661 catgaagaaa gccgccctcg ggcggctttc cattccacgc gcctcgcgcg aagtcagtgc
     3721 gaggaagtct cccggtcgaa ctcctccgcg gtgatgtggc gcacgtcctt gcccttcacc
     3781 aggtagacca cgtactcgga catgttcttg gcgtggtcgc cgatgcgctc cagcgccttg
     3841 gcgacgaaga gaatctctat cgacgtcgat atggtgcgcg gatcttccat catgaacgtg
     3901 atgagctggc gcaggatgcc gcggaactgc tcgtcgagca ggcggtcctg cttgaccacc
     3961 gcggcggccg cggcgatatc gacgcgcgca aaggcgtcca gcgacttctt gagcatctcg
     4021 agcgcgtagc cggcggcgac cttgatctcc gcgaagcgcg gcaggagcat gcgctccgag
     4081 gcgtagatga gcttggtcat gcgcgcgatc ttctcggcct cgtcgccgat gcgctcgagg
     4141 tcggtgatca tcttgatgat cgtcatgatc atgcgcaggt cctgcgcggc cggctggcgg
     4201 cgggcaatga tgtgcgcgca gtcctcgtcg atcctcacct cgagggcgtt cacgcggtgg
     4261 tcgtcggcga tggtctgttc ggcgagcgag atgttgccgg tcgagagcgc ctcgatggcg
     4321 gcggtgatct gcgtctccac caggccgccc atctgcagca cgcgcgagcg cacggcctcc
     4381 agctcggcgt cgtagcgctt cgaggtgtgt tcctgggtat tcaggggcat cgggtgattc
     4441 ctccatcgca tcgcggcctg cccgggcggg atcggcgccg gatgccggat gctaacgcgt
     4501 cattgtgacg caatcgtgac gttcagcgca ggcggtcgat tcggcgcacg ccctgcgcgg
     4561 tgccgagcag gagcacgtcc gcgggacggc gtgcgaagag gccgttggtc acgacgcccg
     4621 gaagctggtt gagttccgac tcgagcgccg ccgggtcgag gatggcgaga ccgtgcacgt
     4681 cgaggatcac gttgccgttg tcggtgacca cgccttcgcg cagcacgggg cgcccgccga
     4741 gcttcacgat ctgccgcgcc acgaggctcc tcgccatcgg gatcacctcc accggcaggg
     4801 ggaacttccc gagcaccgcg acgagcttcg aggcgtccgc gatgcagacg aacctgcggc
     4861 tcgcctgcgc cacgatcttc tcgcgcgtga gggccccgcc gccgcccttg atcatcgcga
     4921 ggctctccgt gacctcgtcc gcgccgtcga catagatatc gcagccgtcg gtactgttca
     4981 ggtcggcgac cggtatcccc gccgcgcgca gccttttcgc gctcgcttcc gaacttgaaa
     5041 cggtcccggc gatgcgcgac ctgcgcgcgg ccagtgcggc gatgaaatgg ttcaccgtcg
     5101 aaccggtgcc cacccccacc atcatgtcgt cctcgacaaa ggcgagggcg gcctccgcgc
     5161 agagcttctt gagggcgtcc tggtccatgg ggccaatcat atattggtat tcttggggtc
     5221 tgggagaatc atgaaaaccg actacctgcg caagatcctg tcggcccgcg tctacgacgt
     5281 ggccatcgaa agccccctcg agctcgcccc cgccatttcg aggcgcactg gcaaccacat
     5341 cctgctgaag cgcgaggaca agcagccggt cttctccttc aagctgcgcg gcgcctacaa
     5401 caagatggcg cacctgaagc ccgagcagct cgcgcgcggc gtgatctgcg cgagcgcggg
     5461 caaccacgcc cagggcgttg cgctcggcgc gcagaagctg gggacgacgg ccaccatcgt
     5521 gatgccggtg accacgccgc gcatcaaggt ctcggcggtg gcggtgcgcg gcgcgaaggt
     5581 ggtcctgcac ggcgacagct accacgaggc cgcgcaacac gcgaaggcga tggcgaagaa
     5641 gcagggcctc acgttcgtgc acccctacga tgatcccctc gtcatcgccg ggcagggcac
     5701 gatcggcatg gagatcctgc gccagcacca gcggcccatc gacgccatct tcgtgccggt
     5761 gggcggcggc ggactcatcg cgggcatcgc gtcctacgtg aagcgcatca cccccgcgac
     5821 ccggatcatc ggcgtggagc cggtggactc caacgccatg accgcgtcgc tcaaggccgg
     5881 ccgccgcgtg acgctgccgc acgtgaacat cttcgccgac ggcgtggcgg tccggcaggt
     5941 ggggcgcgag accttccggc tgtgccggga gctcgtcgac gagatggtgc tggtcgacac
     6001 cgacgagatc tgcgcggcca tcaaggacat cttcgaggac acgcgcaccg tggtggagcc
     6061 ctccggggcc ctcgcgctcg cgggcgccaa gcgctggctc gcgcggcgcc gcctcaaggg
     6121 caggaccgtg gtggcgatcg cctgcggcgc caacatgaac ttcgaccggc tgcgcttcgt
     6181 ggccgagcgc gccgagctgg gcgagcaccg cgaggcggtg ctggcggtga ccatcccgga
     6241 gaagccgggc agcttccgcg c
//
LOCUS       DASUEV010000076         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1511576, whole genome shotgun sequence.
ACCESSION   DASUEV010000076 DASUEV010000000
VERSION     DASUEV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511576"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFV55_02410"
     CDS             complement(<1..738)
                     /locus_tag="VFV55_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4943175.1"
                     /translation="MTAYVIRRMWQMIPTLAGVILLIFFLFNWVGGDPAQVLAGKISN
                     PEQIANIRKQLGVDQPYWYQLWVFVQQVFTFDFGRSWSTNEEVSRILLTRVGPTLTIM
                     VPVMIIETTLAVVFAIMVAYVRGSLTDRTIMVICTTAMSISFLVYIIFGQWLFGFILG
                     WFPVQGWSESFWKNLFTYAPLPIMLAILVGVAPQLRLFRSFFLEEIHQDYVRTARAKG
                     VPEKTVMLKHVLRNAMIPILTNIGISLP"
     gene            complement(751..2577)
                     /locus_tag="VFV55_02415"
     CDS             complement(751..2577)
                     /locus_tag="VFV55_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943176.1"
                     /translation="MRRLAVLAACLACALFAAAPAGAAAERKVLRMALRSAEAGFDPQ
                     RIDDRYSVGVCENFYEPLLTYDYLARPVRLVPLVAETVPEPEEGGTRYTFRLRPGILF
                     ADDPAFKGARRELVAKDVEYAIKRFRDPVNRSPYEWLFEQKIEGLDALAESAKKTGRF
                     DYDAKIPGIDVRDRYTVSFKLVAPDFNFLYVLAMPNVVPVAREVIEAYGADTHAHPVG
                     TGPFVLKEWVRRSKIVLERNPNHRGYELDVRYADMSDPWDRAAVEELAGKRLPLLDRV
                     EIYPIEDEQPRYLAFINREHDLLDELPVAFINQVLPNGRLAPSLARLGVKVFREEQPE
                     FTYDVFNMNDKVDGRDNPVGGYTPDRVALRRAMVLAHDIDREISVIRKGQAIPAQSPI
                     PPGVVGYDPAFRSNGQDYNPPRAKALLDMAGYLDRDGDGWREQPDGRPLVIDYKYTAF
                     NQSNRELAELWVKSMADVGIRMQATAVQFVDLLKDKKVGRFEVSGSAWTADYPDAQNF
                     LQLLYGPNTGQSNDAGFKLAEYDRLYRLSQGVPPGPERNALYHEMIRLMLVWAPYRLG
                     VHRVFNHLIYPWVKGYKKHPILYTNFKYLDLDVAARQAATNR"
BASE COUNT          477 a          848 c          953 g          469 t
ORIGIN      
        1 gggcagcgag atgccgatgt tggtgaggat cgggatcatc gcgttgcgca gcacgtgctt
       61 gagcatgacg gtcttctcgg gcacgccctt ggcgcgcgcg gtgcgcacgt agtcctggtg
      121 gatctcctcg aggaagaagg agcggaacag gcgcagctgc ggggccacgc ccaccaggat
      181 cgccagcatg atcggcagcg gcgcgtaggt gaacaggttc ttccagaagc tctcgctcca
      241 gccctgcacc gggaaccagc cgaggatgaa gccgaagagc cactggccga agatgatgta
      301 caccaggaag ctgatcgaca tggcggtcgt gcagatgacc atgatcgtgc ggtcggtaag
      361 gctgccgcgc acgtaggcga ccatgatcgc gaagacgacg gcgagcgtcg tctcgatgat
      421 catcaccgga accatgatgg tgagcgtggg ccccacccgc gtaagcagga tgcgcgagac
      481 ctcctcgttg gtggaccagc tgcgcccgaa gtcgaaggtg aacacctgct gcacgaacac
      541 ccagagctgg taccagtagg gctggtccac gcccagctgc ttgcggatgt tggcgatctg
      601 ctcggggttg gagatcttgc ccgccagcac ctgcgcgggg tcgcctccca cccagttgaa
      661 caggaagaag atgagcagga tcacgccggc gagcgtcggg atcatctgcc acattcgtcg
      721 gatcacgtag gcggtcacgc ttcttctcct tcagcgattc gtcgccgcct gccgggcagc
      781 gacatcgaga tcgaggtact tgaagttggt gtagaggatc gggtgcttct tgtagccctt
      841 cacccacggg tagatcaggt ggttgaacac ccggtgcacg ccgaggcgat acggcgccca
      901 gacgagcatc agccggatca tctcgtggta gagggcgttt cgctcggggc cgggcggaac
      961 gccctgcgag agccggtaga gccggtcgta ttccgcgagc ttgaagcccg cgtcgttgga
     1021 ctgccccgtg ttgggcccgt agagcagctg caggaagttc tgcgcgtccg ggtagtccgc
     1081 ggtccaggcc gagcccgaca cctcgaagcg gccgaccttc ttgtccttga ggaggtcgac
     1141 gaactgcacg gccgtggcct gcatgcggat gcccacgtcg gccatggact tcacccacag
     1201 ctccgcaagc tcgcggttcg actggttgaa ggcggtgtac ttgtagtcga tcaccagggg
     1261 ccggccgtcg ggctgctcgc gccagccatc gccgtcgcgg tcgaggtagc ccgccatgtc
     1321 gaggagcgcc ttggcgcggg gcgggttgta gtcctggccg ttcgaccgga aggcggggtc
     1381 gtagcccacc accccgggtg gaatggggct ctgcgccggg atcgcctggc ccttgcgaat
     1441 gaccgagatc tcgcggtcga tgtcgtgggc gagcaccatc gcgcggcgaa gcgccacgcg
     1501 gtccggcgtg tagccgccga cgggattgtc gcgcccgtcg accttgtcgt tcatgttgaa
     1561 gacgtcgtag gtgaattccg gctgctcctc gcggaacacc ttgacgccca gcctcgcgag
     1621 cgagggcgcg agccggccgt tgggcagcac ctggttgatg aacgcgacgg gcagctcgtc
     1681 cagcaagtcg tgttccctgt tgatgaacgc gaggtagcgc ggctgttcgt cctcgatggg
     1741 atagatctcg acgcgatcga ggagcggaag gcgcttgccg gcgagttcct cgaccgcggc
     1801 ccggtcccac gggtccgaca tgtcggcata gcggacgtcg agctcgtagc cgcggtggtt
     1861 cgggttcctc tcgagcacga tcttggatcg ccgcacccac tccttgagca cgaagggacc
     1921 ggtgcccacg gggtgggcgt gcgtgtcggc gccgtaggcc tcgatcacct cgcgggcgac
     1981 cggcaccacg ttgggcatcg cgagcacgta caggaagttg aaatcgggcg cgacgagctt
     2041 gaagctcacc gtgtagcggt cgcgcacgtc gatgcccggg atcttcgcgt cgtagtcgaa
     2101 gcggccggtc ttcttcgccg attcagcgag cgcgtccagg ccctcgatct tctgctcgaa
     2161 gagccactcg taggggctgc ggttcaccgg gtcgcggaag cgcttgatgg cgtactccac
     2221 gtccttcgcc acgagctccc gcctggcccc cttgaaggcc gggtcgtcgg cgaacaggat
     2281 ccccggccgc aggcggaagg tgtagcgcgt gccgccctct tccggctcgg ggacggtctc
     2341 ggccaccagg ggcaccaggc gaacgggccg ggcgaggtag tcgtaggtga ggagcggctc
     2401 gtagaagttc tcgcacaccc ccaccgaata gcggtcgtcg atccgctggg gatcgaaccc
     2461 cgcctccgcc gagcgcaatg ccatgcgcag gaccttgcgc tccgctgcgg cgccagccgg
     2521 tgccgcggcg aaaagcgcgc aggcgaggca ggcggcgagg acggcaaggc gacgcatcaa
     2581 gcgggagctc cggagacggg caaatcgcaa attatacggt gtcagacacg gcagccagac
     2641 aaaggggtca ggttacttcg ggaaaagggg tcaggttact tttctgtcgt attgccgtcc
     2701 caagacggct ggctgaaaag taacctgacc ccatttccca aagtaac
//
LOCUS       DASUEV010000077         4828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_239736, whole genome shotgun sequence.
ACCESSION   DASUEV010000077 DASUEV010000000
VERSION     DASUEV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239736"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /gene="lptB"
                     /locus_tag="VFV55_02420"
     CDS             complement(<1..651)
                     /gene="lptB"
                     /locus_tag="VFV55_02420"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX4943177.1"
                     /translation="MVLRAEALMKRYRKRTVVSDVSLTVESGEVVGLLGPNGAGKTTC
                     FYMILGLVPIDQGTVRLDDRDVTHLPIYRRSRLGLSYLPQEASIFRRLNVAQNIQAVL
                     ELQGLEEEEIEARLENLLDDLHIGHLRASPALSLSGGERRRVEIARALATSPRFILLD
                     EPFAGVDPIAVLDIQQIIRFLKERGIGVLITDHNVRETLGICDHAYIINQGSVLAFG"
     gene            complement(705..1325)
                     /gene="lptA"
                     /locus_tag="VFV55_02425"
     CDS             complement(705..1325)
                     /gene="lptA"
                     /locus_tag="VFV55_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX4943178.1"
                     /translation="MRAHATRLVVHTLRLVLAAALAGAISAHAERGDREKEIQVLADR
                     LSADDAKKEAVYEGNVIVTQGSMRITSARIVVREDAQGYRTYIATGAPVTFRQKRDKV
                     DDWIDGSAARAEFDDRNDILKLFNGAKLKSSQGELAGDFISYDRGKEFFEVTGAAPGA
                     PAGAGSRVRATIIPQKKGVDGKPAPAAEAPPVNLKPDKRPGTGGEP"
     gene            complement(1325..1891)
                     /gene="lptC"
                     /locus_tag="VFV55_02430"
     CDS             complement(1325..1891)
                     /gene="lptC"
                     /locus_tag="VFV55_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX4943179.1"
                     /translation="MIRPSSWLPLGVLGMLVALTWWLDQLVQPTPERSDGRMRHDPDL
                     VVENFSARKFGEDGRTLYTLAARKMVHYPDDNSALLEKLGFEAYEPRQPRVTIASDGG
                     RLLEGGDEVWFEGNVVLVREADKRYEASRLTTERLLVLPDAGVARTTGPVLLENPAAR
                     VEASGLELNNQTRIMKLDRVRATYKPRR"
     gene            complement(1888..2415)
                     /locus_tag="VFV55_02435"
     CDS             complement(1888..2415)
                     /locus_tag="VFV55_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX4943180.1"
                     /translation="MPDAVTRARAIRLALFDVDGVLTDGRLYYGPAGEAMKVFDIRDG
                     HGLKMLARSGVVTGLLSGRRSEAAAARARELGMPHVMLGVEDKLAHFDRLLAQLGLEA
                     SQCSFMGDDLPDAPVLARCGLAVAVADAEADVKAIAHLVTRAAGGHGAVREFCEFVMR
                     AQGTLESAAKREGKG"
     gene            complement(2415..3392)
                     /locus_tag="VFV55_02440"
     CDS             complement(2415..3392)
                     /locus_tag="VFV55_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX4943181.1"
                     /translation="MPDSARILALAKEVLEIEAGAVAALTSRLGEQFLAAVEMIFASK
                     GRVVVTGMGKSGHVGGKIASTLASTGTPAFFMHPGEASHGDLGMIAAGDVVIAISNSG
                     ESDEILRILPLIKRRGAKVIAITGRPVSTLAGESDVHLDAAVEKEACPMNLAPTASTT
                     ATLALGDALAVALLEKRGFGSDDYALHHPGGALGRRLLLHVSDVMHTGEKLPRVGVEA
                     TLKEAILEMSQKNLGMTAIVDADGRVAGLFTDGDLRRTLEKHDDIRGLKVAEVMTRNP
                     RSIGPSRLAAEAAQVMQTHHVSGRLLVVDESGRLVGALHVDDLLRAGVI"
     gene            3546..>4828
                     /locus_tag="VFV55_02445"
     CDS             3546..>4828
                     /locus_tag="VFV55_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX4943182.1"
                     /translation="MPAILGYLLVGAVIGPGALGLVSASEDKRYLAEFGVVFLMFSVG
                     LEFSLPQLMAMRRTVFGYGGAQVTLAMVLAAAVALAAGASWQTGLIVGGVLAMSSTAI
                     VSKSLSEQAKLHTPAGRQIMGVLLFQDLAVIPLLVLIPALGRPAEALASSMVLALAKA
                     AVVLAVVLFLGQRLMRPLFHLVAKQKSSELFVLFVLLVTLGLAWVTELAGLSLVLGAF
                     LAGMLISETEYRYQVDDYIKPFRDVLLGFFFVTIGMLLDLRVVMANWFYVALVLVALL
                     VAKFVLVFWLGRVFGNEKPTALRCALALAPAGEFGFVLLSLAGREGALDPATLQVVLA
                     GALISMLATPVLLANTERIVLYVVESEWTQRAMALHQLAVRTMATQGHVIVCGYGRSG
                     QALSRFLEREGVSVIALDADPERVRQAAAAGDSVV"
BASE COUNT          656 a         1630 c         1689 g          853 t
ORIGIN      
        1 cccgaaggcg aggaccgagc cctggttgat gatgtaggca tggtcgcaga tgcccagcgt
       61 ctcccgtacg ttgtggtcgg tgatgagcac gccgatgccg cgctccttga ggaagcggat
      121 gatctgctgg atgtccagga cggcgatcgg atcgacgccg gcgaagggtt cgtcgagcag
      181 gatgaagcgc gggctggtgg cgagtgcccg cgcgatctcg acgcggcgac gctcgccgcc
      241 ggaaagggac agtgccgggc tcgcgcgcag gtgaccgatg tgcaggtcgt cgagcaggtt
      301 ctccaggcgc gcctcgatct cctcttcctc cagtccctgg agttccagga cggcctggat
      361 gttctgcgcg acattgaggc ggcggaatat cgacgcttcc tgcggcaggt aggaaagccc
      421 gagccgcgag cggcggtaga tgggaaggtg ggtgacgtcc cggtcgtcga gccggaccgt
      481 tccctggtcg atcggcacga ggccgaggat catgtagaag caggtggtct tgcccgcgcc
      541 gttgggcccg aggagcccta ccacctcgcc gctctccacg gtcagcgaga cgtccgagac
      601 cacggtgcgc ttccggtagc gcttcatgag cgcctcggcg cgcagcacca tccgcttcgg
      661 ctcgctcttc acgagcgtga gtggctgggc gacggtgctt gcgctcacgg ctcgccgcca
      721 gtccccgggc gcttgtcggg cttgaggttg accggcggcg cttccgcagc cggggccggt
      781 ttgccgtcca ctcccttttt ctgcggaatg atggtcgcgc gcacgcgcga accggcgccg
      841 gcaggagcgc cgggcgcggc gccagtgacc tcgaagaact ccttgccgcg gtcgtaggag
      901 atgaaatcgc cggccagctc gccctgggag ctcttcagct tcgccccgtt gaacagcttc
      961 aggatgtcgt tgcggtcgtc gaattcggcg cgcgcggcgg agccgtcgat ccagtcgtcg
     1021 accttgtcgc gcttctggcg gaaggtgacc ggggcgcccg tcgcgatgta ggtgcgatag
     1081 ccctgcgcat cctcgcgcac gacgatcctc gcggaggtga tgcgcatcga gccctgcgtg
     1141 acgatgacgt tgccctcgta gaccgcctcc ttcttcgcgt cgtcggccga aaggcgatcg
     1201 gcgagcacct ggatctcctt ctcgcggtcg cccctctcgg cgtgcgcgga aatcgcgccg
     1261 gccagggccg cggcaagcac gagacgcagg gtgtggacga cgaggcgggt ggcgtgtgcg
     1321 cgcatcaacg gcggggcttg taggtggcgc gcacgcgatc gagcttcatg atgcgcgtct
     1381 ggttgttcaa ttccaggccc gaggcctcga cgcgggcggc ggggttttcg agaagcacgg
     1441 ggccggtcgt gcgggccacg ccggcgtcgg gaaggacgag gagccgttcc gtggtgagcc
     1501 tggaggcctc gtagcgcttg tcggcctcgc gcaccagaac gacgtttccc tcgaaccaga
     1561 cctcgtcgcc gccctctagc agccggccac cgtccgacgc gatcgtcacg cggggctggc
     1621 gcggctcgta cgcctcgaaa ccgagcttct ccagcagtgc cgagttgtcg tccgggtaat
     1681 gtaccatctt gcgcgccgcc agggtgtaga gcgtcctgcc gtcttcaccg aacttgcgcg
     1741 cgctgaaatt ctcgactacc aggtccgggt cgtggcgcat ccttccgtcg ctgcgctccg
     1801 gcgtgggctg caccaactgg tccagccacc aggtgagcgc gaccagcatc ccgaggacgc
     1861 cgagcggcag ccaggaagag gggcggatca tcccttgccc tcgcgtttcg cggcgctctc
     1921 caaggtgcct tgcgcgcgca tcacgaattc gcagaactcg cgcaccgccc cgtgtccgcc
     1981 ggcagcccgg gtgacgagat gcgcgatcgc cttcacgtcc gcctccgcat cggctacggc
     2041 gaccgcgagc ccgcagcgcg ccagcaccgg cgcatccggg agatcgtcgc ccatgaacga
     2101 gcactgcgat gcctcgagcc ccagttgcgc gagcagccgg tcgaagtgcg cgagcttgtc
     2161 ctcgacaccg agcatcacgt gcggcattcc cagttcccgt gcgcgcgccg cggcggcttc
     2221 cgagcggcgg ccggagagca agccggtgac cacgcccgat cgcgcgagca tcttcaggcc
     2281 gtgcccgtcg cggatgtcga acaccttcat cgcctcgccg gctggtccgt agtaaaggcg
     2341 cccgtcggtg agcacgccgt cgacgtcgaa gagcgcgagg cggatggcgc gggcgcgggt
     2401 gactgcgtcg ggcatcaaat cacaccggcc cgcagcaggt catcgacgtg cagcgcgccc
     2461 acgagccggc cggattcgtc gacgacgagg agccgtccgc tgacatggtg cgtctgcatc
     2521 acctgcgcgg cctcggcggc caggcgcgac gggccgatcg agcgcgggtt cctcgtcatc
     2581 acctcggcga ccttgaggcc gcggatgtcg tcgtgcttct cgagcgtgcg gcgcaggtcg
     2641 ccatcggtga agagccccgc gacgcgcccg tcggcgtcga cgatcgcggt catgccgagg
     2701 ttcttctgcg acatctcgag aatcgcctcc ttgagcgtgg cctccacgcc cacgcgcggg
     2761 agcttttccc cggtgtgcat cacgtccgag acgtgcagga gcagccgccg gccaagcgcg
     2821 cctcccgggt ggtgcagcgc atagtcgtcg ctgccgaacc cgcgcttctc caggagggcc
     2881 acggcgagtg cgtcgccgag ggcgagcgtg gccgtcgtgc tcgcggttgg cgcgaggttc
     2941 atcgggcacg cttccttctc caccgcggcg tcgaggtgca cgtcggactc gcccgcgagc
     3001 gtcgataccg gccggccggt gatcgcgatc actttcgcgc ctcggcgctt gatgagggga
     3061 aggatgcgca ggatctcgtc ggattcgccg gagttggaaa tcgcgatgac cacgtcgcct
     3121 gcggcgatca tcccgaggtc gccgtggctc gcctccccgg ggtgcatgaa gaacgcgggc
     3181 gttccggtgg aggcgagggt ggaggcgatc ttgccgccga cgtgcccgga cttccccatg
     3241 ccggtcacga cgacgcggcc cttcgacgcg aagatcatct cgaccgccgc caggaactgc
     3301 tcgccgaggc gactggtgag cgccgccacg gcgccggcct cgatttcgag cacttccttc
     3361 gcaagcgcca ggatgcgcgc ggagtcgggc atcggccggg gatggtggag atttgtcacg
     3421 gaggaaagta taaagggatt gccccacccc cgccgccctc gcccaagatg catgacgccc
     3481 ttcccgtagc actcatcctt ctcggttccg cggttgcggt cgtcgtgatt ttccggcgcc
     3541 tgtccatgcc tgcgatcctc ggttatctgc tcgtgggcgc ggtcatcggc ccgggcgcgc
     3601 tggggctggt ctcggcttcc gaggacaagc gctacctcgc cgagttcggg gtcgtcttcc
     3661 tcatgttctc ggtgggcctc gagttcagcc tgccgcaatt gatggccatg cggcgcaccg
     3721 tgttcggcta tggcggcgcc caggtgaccc tcgcgatggt gctcgcggcg gccgtggcgc
     3781 tcgccgcggg cgcgtcgtgg cagacggggc tcatcgtggg cggcgttctc gcgatgtcgt
     3841 cgaccgccat cgtctccaag tcgctctccg agcaggcaaa gctgcacacg ccggcggggc
     3901 gccagatcat gggcgtgctc ctgttccagg acctcgccgt cattccgctg ctggtcctca
     3961 ttccggcgct cgggcgcccg gcggaggcgc tggcaagctc gatggtcctc gcgctcgcga
     4021 aggcggcagt ggtcctggcg gtggtgctct tcctcggcca gcggctgatg cgcccgctct
     4081 tccacctcgt ggcgaaacag aagtcgtccg agctcttcgt cctcttcgtg ctgctcgtca
     4141 ccctcgggct ggcgtgggta acggaactcg ccgggctttc gctcgtgctg ggcgccttcc
     4201 tcgccggcat gctcatctcc gagaccgagt accgctacca ggtggacgac tacatcaagc
     4261 ctttccgcga cgtcctgctg ggtttcttct tcgtcaccat cggcatgctg ctcgacctgc
     4321 gcgtggtgat ggcgaactgg ttctacgtgg cgctggtgct ggtggcgctc ctggtggcga
     4381 agttcgtgct cgtgttctgg ctggggcgcg tcttcggcaa cgagaaaccg acggcactgc
     4441 gctgcgcgct ggcgctcgcg ccggcgggcg agttcggttt cgtgctgctc tccctggcgg
     4501 gccgcgaggg cgcgctggac ccggccacgc tccaggtggt tctcgcgggc gcgttgatct
     4561 cgatgctggc cacgcccgtg ctgctcgcca acacggagcg catcgtcctg tacgtcgtcg
     4621 aaagcgaatg gacgcagcgt gccatggcgc tgcaccagct ggcggtgcgg accatggcca
     4681 cgcagggcca cgtcatcgtc tgcggctacg gtcgcagcgg ccaggcgctt tcccggttcc
     4741 tcgagcgcga aggcgtgtcg gtcatcgcgc tggacgcgga tcccgagcgc gtccgccagg
     4801 cagcggccgc gggcgattcg gtggtcta
//
LOCUS       DASUEV010000078         5739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1390523, whole genome shotgun sequence.
ACCESSION   DASUEV010000078 DASUEV010000000
VERSION     DASUEV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390523"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFV55_02450"
     CDS             complement(<1..620)
                     /locus_tag="VFV55_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4943183.1"
                     /translation="MQSHPPAWLRWAPFLFVFLWSTGFIGSRLGAPYAEPLTFLSWRY
                     AVAVTLMLAAALATRAAWPASPMAAGHAAVAGLLVHGAYLGGVFCGVARGMPLATVSL
                     IVGLQPVLTAMAAGPLLGERLRRSQWLGIALGFAGVVLVVAGKWSGAASDATAYAWAF
                     FALAGITAGTLYQKRFCGAVPMLAGGVIQYTAAGIVTFAAACATET"
     gene            785..2035
                     /locus_tag="VFV55_02455"
     CDS             785..2035
                     /locus_tag="VFV55_02455"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4943184.1"
                     /translation="MRSLATCLALGLSAGAFAQPLPVTTADKAGFSAEGLARIDAFFA
                     REIAADRVPGAVVAIARDGKLVHYKAYGFLDKGAGKPMPLDAMFNLASMTKIMASVGA
                     LTLNEEGRLPLKSRLDEYLPEFKDVKVGVVSPAGEVTLEAPRQPIFIHDLMRHTSGFT
                     YGNANHPVRKQYPGAFDATDTPGPEFLDKIARAPLVNQPGTIFEYGVSADVLGFVIEK
                     ISGQRLGEFLKVRIWDKVGMKDTMFVVPESRMARLAQPLPVDPISGKPQAIKILKTQS
                     KFDCAGACAFGTVGDFLRFGQMLVNGGTIDGKRVLSPKTVAFMTSNHLTASIKNNVPL
                     VEPHRDGYSFGLGVAVRGFDGVAATPGTAGDYSWNGANGTSFWADPKEKLVVVVGTVA
                     PGDIRKYYREQMGALVYGAMSRLK"
     gene            2079..3662
                     /locus_tag="VFV55_02460"
     CDS             2079..3662
                     /locus_tag="VFV55_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4943185.1"
                     /translation="MNLALWLERHARVDPSRTAVFVGASPWKSYARLAADAASLARGL
                     RGKLGLAAGDRVAVVMTNAPEYPEVLLGIWWAGLAAVPVNAKLHPREVAFILEDSGAR
                     ATFATPEWMAGIAQATSALAAPPVVLEAGSPDYRALFGDGMPPVAVEDGALAWIFYTS
                     GTTGRPKGAMLSHGNLRAMAQGYLTDVDVVGPDDRLLHAAPMSHGSGLYLVPYLLAGA
                     AQVIPESGGFDPAEVFALAGAHRGVGMFAAPTIVRRMVEHAAMHAPSLEGLRTVVYGG
                     GPMYVADLLRALEVMGPRFAQIYGQGESPMTITALARRFVGDRSHPRHLARISSVGVA
                     QSGVEVVVRDERGCDLPPGEVGEVCVRGAVVMSGYWMNPEATAKSLRDGWLWTGDLGA
                     FDEEGFLALKDRSKDLIISGGSNIYPREVEEALLTHPGVAEVSVVGRADPEWGEAVVA
                     FIVARGPRLPAADLDAHCLAAIARFKRPKEYRFVSELPKNNYGKVLKTELRAMLAREE
                     PGAGTAAPGTPASRTIIPK"
     gene            3748..4344
                     /gene="wrbA"
                     /locus_tag="VFV55_02465"
     CDS             3748..4344
                     /gene="wrbA"
                     /locus_tag="VFV55_02465"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX4943186.1"
                     /translation="MARVLVLYYSSYGHIEQMAEAVAQGVREAGSEAIVKRVPETVPE
                     DVARKSGFKLDQKAPVATVEELPEYDAIIFGTPTRYGNMTAQMKTFLDRTGALWMKGS
                     LIGKVGSVFTSSATQHGGQESTILTFHPVLLHLGMVIVGLPYSFQGQMGYGEVVGCSP
                     YGASTIAGGDGSRQPSKIELEGARFQGRHVAQIAAKLH"
     gene            complement(4407..4832)
                     /locus_tag="VFV55_02470"
     CDS             complement(4407..4832)
                     /locus_tag="VFV55_02470"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEX4943187.1"
                     /translation="MKRFLAAFSFAILALAANAQQKPEAIDDQQFSLNKGNVFEVATP
                     RPFEFEDGAKTLRPLEGSGMPVMISHTIDDDLPITMEKNGCIRCHTPTGKKVRGAPAV
                     PPSHVAAGDKPAVAGKRWNCIACHAPQADVKPVVANTAR"
     gene            complement(4829..>5739)
                     /gene="napH"
                     /locus_tag="VFV55_02475"
     CDS             complement(4829..>5739)
                     /gene="napH"
                     /locus_tag="VFV55_02475"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HEX4943188.1"
                     /translation="RPVRHFEMPSSLAGKVHAWRFLIARRAVQAAVLLAFFGTAHWGW
                     KAMGRPFLAGNLSAASLAGKIPLADPFAVLEVLATRHLVVSEALLGAAIVFVIYFLLG
                     GRVFCAWVCPMNVVTDAARWVRRRLAVRDVFAIPPGVRYAVLALALVLSAIAGVAAFD
                     WISPIAMLHREILYGAGLGLTSALGVFLFDAFVLKHGWCGHLCPLGAFWSLAGRGAQV
                     RVAFDDATCTRCGDCLKVCPEPRVLNFNEAARAGMVRHGECTNCAACIAVCPESSLRF
                     TLRAAVRAYPVHVPGTKPFPTEGMTP"
BASE COUNT          959 a         1930 c         1998 g          852 t
ORIGIN      
        1 ctcgtctcgg tcgcgcaggc ggcggcgaag gtgacgatgc ccgcggcggt gtactggatc
       61 acgcctccgg cgagcatcgg aacggcgccg cagaagcgct tctggtagag cgtgccggcg
      121 gtgatgcccg ccagcgcgaa aaacgcccat gcgtacgcgg tggcatcact tgccgcgccg
      181 ctccacttgc ccgcgacgac gagcaccacg cccgcgaaac cgagcgcgat gcccagccat
      241 tggctgcgcc tgagacgctc gcccagcagc ggcccggcgg ccatcgccgt gaggacgggc
      301 tgcaggccga cgatgagcga aaccgtcgcg aggggcatgc cgcgcgccac gccgcagaac
      361 acgcccccca ggtaagcgcc gtgcaccagg agtcccgcga cggccgcgtg tcccgccgcc
      421 atgggcgacg cgggccatgc cgcgcgcgtc gcgagtgccg cggcgagcat caacgtcacg
      481 gcaacggcgt accgccacga aaggaacgtg agcggctccg cgtagggcgc gcccaggcgg
      541 ctgccgatga agcccgtgct ccacaggaag acgaagagga aaggcgccca gcgcaaccag
      601 gcaggaggat gggattgcac gggcggcgcg caggagcgtt gcggagttgc gattctaacc
      661 gcgcttcccc ggccgcccca aaggaaacgc tccatcggcc cgcgtcgatg gcgggccgat
      721 gcgtggagtc gtaatatgtc cgccggcctc gatgcccccc gactcacgaa aaggaaaaca
      781 gaaaatgcgt tcgctcgcga cttgccttgc tctcggcctc agcgccggtg cgttcgccca
      841 gccattgccc gtgaccacgg ccgacaaggc cggcttctcc gccgaaggcc ttgcgcgcat
      901 cgacgcgttc ttcgcccgcg agattgcggc cgaccgcgtg cccggcgccg tcgtggccat
      961 tgcgcgcgac ggcaaactcg tccactacaa ggcctatggc ttcctcgaca agggcgccgg
     1021 caagcccatg cccctcgatg cgatgttcaa cctcgcctcg atgaccaaga tcatggcgag
     1081 cgtgggcgcg ctcacgctca acgaggaagg gcgcctgccg ctcaagagcc gcctcgacga
     1141 gtacctgccg gaattcaagg acgtgaaggt gggcgtggtg tctcccgcgg gcgaagtgac
     1201 cctggaggca cccaggcagc ccatcttcat ccacgacctg atgcgccata cctcgggctt
     1261 cacctatggc aatgcgaacc atccggtgcg caagcagtat ccgggcgcgt tcgacgcgac
     1321 ggataccccg ggccccgagt tcctcgacaa gatcgccagg gccccgctcg tgaaccagcc
     1381 cggcacgatc ttcgagtacg gcgtctccgc cgacgtgctg ggcttcgtga tcgagaagat
     1441 cagcggccag cggctcggcg agttcctgaa ggtgcgcatc tgggacaagg tcggcatgaa
     1501 ggacacgatg ttcgtcgtgc ccgaaagccg gatggcgcgg ctcgcgcaac cgctgcccgt
     1561 cgacccgatc tcgggcaagc cgcaggcgat caagatcctc aagacgcaat cgaagttcga
     1621 ttgcgcgggc gcttgcgcct tcggcaccgt cggcgacttc ctgcgcttcg gccagatgct
     1681 cgtgaacggc gggaccatcg acggcaagcg cgtgctgagc ccgaagacgg tggccttcat
     1741 gacttcgaac cacctgacgg catccatcaa gaacaacgtg ccgctcgtcg agccgcatcg
     1801 cgacggctat agcttcggcc tcggcgtcgc cgtgcgcggc ttcgacggcg tggcggccac
     1861 tccgggcaca gccggtgact attcgtggaa tggcgccaac ggcacctcgt tctgggccga
     1921 tccgaaggag aagctcgtcg tcgtggtggg cacagtggca ccgggcgaca tccgcaagta
     1981 ttaccgggag cagatgggcg cgctggtcta cggcgcgatg tccaggctca agtaggcgca
     2041 atatctcgcg gtgccagaca ccgtgccagg cactgtccat gaacctcgca ctctggctcg
     2101 aacgccacgc ccgcgtcgat ccgtcgcgga cggcagtatt cgtcggtgcg tcgccgtgga
     2161 agtcgtacgc ccgactcgcg gcggatgcgg catcgctggc ccggggactg cgcgggaagc
     2221 ttggcctcgc ggccggcgat cgcgttgccg tcgtcatgac caacgcgccc gagtatccgg
     2281 aggtattgct cgggatctgg tgggccggtc ttgccgcggt gccggtgaac gccaagctgc
     2341 atccgcgcga agtggccttc atcctcgaag actccggtgc gcgtgcgacg tttgcgacac
     2401 cggagtggat ggcgggaatc gcgcaggcaa cctccgcgct cgccgcaccc cccgtcgtac
     2461 tcgaagcagg cagtccggac taccgcgcgc tgttcggcga cggcatgccg cccgtcgcgg
     2521 tggaggatgg cgcgctcgcc tggatcttct acacgagcgg caccaccggc cggccgaagg
     2581 gggcgatgct ctcgcacggc aacctgcggg cgatggcgca gggctacctc acggatgtcg
     2641 atgtcgtcgg tcccgacgat cgcctgctcc atgcggcacc catgtcgcac ggttcgggtc
     2701 tctacctcgt cccctacctg ctggcgggcg ccgcgcaggt cattcccgag tccggcggct
     2761 tcgatcccgc ggaggtgttc gccctcgcgg gggcgcatcg cggcgtcggc atgttcgctg
     2821 cgcccacgat cgttcgccgc atggtcgaac atgcggccat gcacgcgcct tcgctcgagg
     2881 gcctgcgcac cgtggtctac ggcggcgggc cgatgtacgt ggcggacctg ctgcgggccc
     2941 tcgaggtgat gggcccgcgt ttcgcgcaga tctacgggca gggcgagagc ccgatgacca
     3001 tcaccgcgct cgccaggcgc ttcgtgggcg atcgaagcca cccgcgccac ctcgcgcgca
     3061 tttcctcggt gggcgttgcg cagagcggcg tggaagtcgt cgtgcgcgac gaacggggat
     3121 gcgacctgcc gccgggcgaa gtcggcgagg tctgcgtgcg cggggccgtg gtgatgtcgg
     3181 gctactggat gaatccggag gccacggcga aatcgctgcg cgacggctgg ctgtggaccg
     3241 gcgaccttgg cgccttcgac gaggagggct tcctcgcgct caaggaccgc agcaaggacc
     3301 tgatcatcag cggcggctcc aacatctacc cgcgcgaggt ggaggaagcg ctgctcacgc
     3361 atcccggcgt ggccgaggtc tcggtggtgg gccgcgcgga cccggagtgg ggcgaggccg
     3421 tcgtcgcgtt catcgtggcg cgcgggccgc gattgcctgc cgcggatctc gacgcgcact
     3481 gcctcgcggc aatcgcgcgt ttcaagcggc cgaaggagta ccgcttcgtg agcgagctcc
     3541 cgaagaacaa ctacggcaag gtgctcaaga ccgagttgcg cgcgatgctc gcgcgcgagg
     3601 aaccgggcgc aggaacggcg gcgcccggga cgcctgcgtc ccgaacgatc atcccgaaat
     3661 gaccatcgct ggaccgtcct ggcctcccgg ccggggcagg acggaattca acctggcctg
     3721 atcgcccgac ctggcaagga ggggaagatg gcaagagtgc tcgtcctgta ttactcgtcg
     3781 tatggtcaca tcgagcagat ggccgaagcg gtcgcgcagg gcgtgcgcga ggccggctcc
     3841 gaagccatcg tcaaacgcgt gccggaaacc gttcccgagg acgtcgcccg gaaatccgga
     3901 ttcaagctcg accagaaggc gccggtcgcc accgtggagg aactgccgga gtacgacgcg
     3961 atcatcttcg gcaccccgac gcggtacggg aacatgaccg cgcagatgaa gaccttcctc
     4021 gaccgtaccg gcgcgctgtg gatgaagggc tcgctcatcg gcaaggtcgg ctcggtgttc
     4081 acctcgtcgg ctacccagca cggcggccag gaatcgacca tcctcacctt ccaccccgtc
     4141 ttgctgcacc tgggcatggt gatcgtcggc ctgccctatt ccttccaggg gcagatgggg
     4201 tatggcgagg tggtgggctg ctcgccctat ggcgcgtcca ccatcgcggg cggggacggc
     4261 agccgccagc cttcgaagat cgagctcgag ggagcccgct tccagggccg gcacgtggcc
     4321 cagatcgcgg cgaagttgca ctgacccgcg agcatcgcgt tccgcatccc cttgccgtcg
     4381 cgcagcggcg agggggcgca ggaagtttac cgggcggtgt tggccacaac cggcttcaca
     4441 tcggcctgcg gcgcgtggca ggcgatgcag ttccagcgct tgccggccac ggccggcttg
     4501 tcgcctgcgg ccacgtgcga cggcggcacg gccggcgccc cacggacttt cttgcccgtt
     4561 ggcgtgtggc agcggatgca gccattcttc tccatcgtga tgggcaggtc gtcgtcgatg
     4621 gtgtgcgaga tcatcaccgg catcccggag ccctcgagcg ggcggagcgt cttcgctccg
     4681 tcctcgaact cgaagggccg gggcgtggcg acctcgaaga cgtttccctt gttgaggctg
     4741 aactgctggt cgtctatggc ctccggcttc tgctgggcgt tggcggcgag cgcgaggatc
     4801 gcgaacgaga aggcggcgag gaagcgtttc atggcgtcat tccttcagtc ggaaagggtt
     4861 ttgtacccgg tacatgtacc gggtacgcgc gcacggcggc gcgcaaggta aagcggaggc
     4921 tggattcggg gcacacggcg atgcatgcgg cgcaattggt gcactcgccg tgccggacca
     4981 ttccggcgcg ggcggcctcg ttgaagttga gcacgcgcgg ctccgggcat accttgaggc
     5041 agtcgccgca gcgcgtgcag gtagcgtcgt cgaaggccac gcgcacctgc gcgccgcgcc
     5101 cggccagcga ccagaacgcg cccagcgggc acaggtggcc gcaccagccg tgcttgagca
     5161 cgaaggcatc gaagagaaag acgcccagcg cggaggtgag acccaggcct gcgccgtaga
     5221 ggatctcccg gtgcagcatc gcgatggggc tgatccagtc gaacgcggcc acgccggcga
     5281 tggccgaaag aacgagagca agggcgagga cggcatagcg aacgccgggc gggatggcga
     5341 acacgtcgcg cactgcaagg cgccggcgaa cccagcgcgc ggcgtcggtc accacgttca
     5401 tcgggcagac ccacgcgcag aacacgcgcc cgcccaggag gaagtagatc acgaagacga
     5461 tggccgcacc gagaagcgcc tcacttacga ccaggtggcg cgtggcaagc acctccagta
     5521 ccgcgaacgg gtcggccagc ggaatctttc ccgccaggct cgcggcggag aggtttcccg
     5581 cgaggaacgg ccggcccatt gccttccagc cccagtgcgc ggtgccgaag aaggcgagca
     5641 gcacggccgc ctgcacggcg cgccgcgcga tcaggaagcg ccaggcgtgc accttgcccg
     5701 caagcgacga gggcatctcg aagtggcgca cgggccggc
//
LOCUS       DASUEV010000079         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_23060, whole genome shotgun sequence.
ACCESSION   DASUEV010000079 DASUEV010000000
VERSION     DASUEV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23060"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1474)
                     /locus_tag="VFV55_02480"
     CDS             complement(161..1474)
                     /locus_tag="VFV55_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4943189.1"
                     /translation="MATVIATNETILVVGGGISGMTAALEAAECGKQVVLVEKSPTLG
                     GRTALLNRYFPKLCHPTCGLEINLRRLRGNRNVRTLTLAEVVSVEGSRGDYTATVRVS
                     PRYVNENCTGCGDCARAVSAEIANPFEYGLAKTKAAYLPHAMAYPARYVLDPSIAGTA
                     EGEKARAACKYGAIDFGMREETIHLKVGAVVWATGWRPYDAARIQPYGYGRFPNVITS
                     LEFERLADTRGPTGGKLLRPSDGQPAKNIAFIQCAGSRDENHLRHCSRICCMASLKQT
                     RYVREAAGDEGRSTIYYIDIRAIDRFEDFYKAVQADPNVAFVKSKVARIAEDRASRDL
                     VLHGVDTEGYHRYATTHDLVVLAVGMEPETRGMKLPDDVIVDSSGFIEGSRDGGQFGA
                     GSAASPLDVNRSVQSATAATLRAIRVIHQAAAAEKSGTVPNITHG"
     gene            complement(1671..1871)
                     /locus_tag="VFV55_02485"
     CDS             complement(1671..1871)
                     /locus_tag="VFV55_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943190.1"
                     /translation="MTLDKARELLRVQVSFGGAYNRNGARNILDAVEREHGAQAADSL
                     LAEFRLDELFGLKPFHSRQQSR"
     gene            complement(1868..2257)
                     /locus_tag="VFV55_02490"
     CDS             complement(1868..2257)
                     /locus_tag="VFV55_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943191.1"
                     /translation="MISPFTLDEVGLVSSLLRSRYLSDTPLEEVEADVQLPPENSAPV
                     ACPGLYWEARGAHFLVVKVGERRYVAQFFYGEKEQFGTGRPEYRNLGECITVLLRLQS
                     DHERTRASALKDAAEPADDEYHGPPVI"
     gene            2256..>2692
                     /locus_tag="VFV55_02495"
     CDS             2256..>2692
                     /locus_tag="VFV55_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4943192.1"
                     /translation="MVAQSNHTAAPTQGPAAQCEVRKTTCYMCACRCGIRVHLRDGEV
                     RYIDGNPDHPLNKGVICAKGASGIMKQYSPARLTRPLRRKNGAERGAGEFEAISWEEA
                     FSLLAERLGKIRSTDPRKFALFTGRDQMQALTGLFARQFGTPN"
BASE COUNT          419 a          886 c          927 g          460 t
ORIGIN      
        1 ctcgtcgcga atggaggcca cgccggcttc cgagcagagg aactcgtggg tgtggacgag
       61 cgcgatcttg ccttccttct gcgcgatctt cgcgagctgc tgcaggtcga gccgttcggc
      121 cagtccgcat ccgccgcaca cgtacgctgc aagtttgttc tcagccatgg gtaatattgg
      181 ggacagtccc tgatttttct gccgctgcag cctggtggat cacgcggatc gcgcgaaggg
      241 tcgccgccgt ggcgctctgc accgagcggt tcacgtcgag cgggctcgcg gcggatcccg
      301 cgccgaactg gccgccgtcg cgcgagccct cgatgaaccc gctcgagtcg acgatcacgt
      361 cgtcgggcag cttcatgccg cgcgtctcgg gctccatgcc gaccgccagg acgacgaggt
      421 cgtgcgtcgt cgcgtagcgg tggtagccct cggtgtcgac gccgtgcagc acgaggtcgc
      481 ggctggcgcg atcctcggcg atgcgcgcca ccttcgactt cacgaacgcc acgttcgggt
      541 cggcctgcac cgccttgtag aaatcctcga agcggtcgat ggcgcggatg tcgatgtagt
      601 agatcgtgga gcgcccctca tccccggcgg cctcgcgcac gtaacgcgtc tgcttgagag
      661 aggccatgca gcagatgcgc gaacagtggc gcaggtggtt ctcgtcccgc gacccggcgc
      721 actggatgaa ggcgatgttc ttcgccggct ggccgtccga gggacgcagg agcttgccgc
      781 cggtggggcc gcgcgtgtcg gcgagccgct cgaactccag gctcgtgatc acgttgggga
      841 accggccgta gccgtagggc tggatgcgcg ccgcgtcgta gggacgccat ccggtggccc
      901 acacgacggc gccgaccttg agatggatcg tctcctcgcg cattccgaag tcgatcgcgc
      961 cgtacttgca ggcggcgcgg gccttctcgc cctcggcggt gccggcgatg gaaggatcga
     1021 gcacgtagcg cgcgggatag gccatcgcgt gcggcaggta cgccgccttg gtcttcgcga
     1081 ggccgtactc gaacgggttg gcgatctccg cgctcactgc cctggcgcag tcgccgcagc
     1141 cggtgcagtt ctcgttcacg taacggggag agacacgtac ggtggcggtg tagtccccgc
     1201 gggacccctc gacggaaacg acttccgcga gcgtgagggt gcgcacgttg cggttgccgc
     1261 gcagccggcg aaggttgatc tcgaggccgc acgtggggtg gcagagcttc ggaaagtagc
     1321 ggttgaggag cgcggtccgg ccgcccagcg tcgggctttt ctccaccagc accacctgct
     1381 tgccgcactc ggccgcctcc agggctgccg tcatgccgct gatacccccg cccaccacga
     1441 gaatcgtctc gttcgtggca atgaccgtcg ccatttcgtc ctcgagttga ctgcgaacaa
     1501 catcgtcccg cccttgcggg acaggatagc cagagcctcg accttatgtc ctgccccccc
     1561 atgccggtca attaccccga tgggctacgc gtacccctta ggagtgatgg acttcccggg
     1621 tgggttgccg cgaaatcggg gcggcccaaa tcacctgcat ttagtggggg ttaccgagat
     1681 tgttgccgtg aatgaaatgg tttcaggccg aagagctcgt cgagccggaa ttccgccagc
     1741 agggagtcgg cggcctgcgc gccgtgctcg cgctcgaccg cgtccaggat gttgcgcgcg
     1801 ccgttgcggt tgtatgcccc gccgaaggac acctgcacgc gcaggagctc gcgtgccttg
     1861 tcgagcgtca tatgaccggg gggccgtggt attcgtcgtc ggccggttcg gcggcgtcct
     1921 tgagcgcgct tgcgcgcgtg cgctcgtgat cggattgcag gcgaaggagc acggtgatgc
     1981 actcgccgag attgcggtac tcgggccggc cggtgccgaa ctgctccttc tcgccgtaga
     2041 agaactgcgc gacgtagcgg cgctcgccga ccttgaccac gaggaaatgc gcgccgcgcg
     2101 cctcccagta gaggccgggg caggcgaccg gcgcggagtt ctcgggagga agctgcacgt
     2161 cggcctcgac ctcctcgagc ggcgtgtcgg agagatatcg gctgcgaagc agcgacgaga
     2221 cgaggccgac ctcgtcgaga gtgaacgggg aaatcatggt tgcccaatct aaccacaccg
     2281 ccgcgccgac gcaaggcccg gccgcgcaat gcgaggtccg caagaccacg tgctacatgt
     2341 gcgcgtgccg ctgcggcatc cgcgtccact tgcgtgacgg cgaggtgcgc tacatcgacg
     2401 gcaaccccga ccacccgctc aacaagggcg tgatctgcgc caagggcgcc tccggcatca
     2461 tgaagcagta ctcgcctgcg cggctcacgc gaccgctgcg gcgcaagaac ggcgccgagc
     2521 gtggcgcggg cgagttcgag gcgatcagct gggaggaggc gttttcgctc ctcgccgagc
     2581 gcctgggaaa gatacgctcg accgatccga ggaagttcgc gctcttcacc ggccgcgacc
     2641 agatgcaggc gctgaccggc ctcttcgccc ggcagttcgg cacgccgaac ta
//
LOCUS       DASUEV010000080         9531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_711119, whole genome shotgun sequence.
ACCESSION   DASUEV010000080 DASUEV010000000
VERSION     DASUEV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711119"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..556
                     /locus_tag="VFV55_02500"
     CDS             32..556
                     /locus_tag="VFV55_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX4943193.1"
                     /translation="MRRWLLLCAAALMLVSCSSEIDELRQFVKNSEKSLPRKIDPLPA
                     VKPFEPFTYEGFDLPDPFKPRKITAREGAAGGVAPDLNRRKEPLEAYPLEQLKMVGTL
                     QQGKQMFAIVRAEKTLYRVKKGNYMGQNFGLITDITDADIKLKEIVQDSAGDWAERQS
                     VLPLLEETGKGDRK"
     gene            553..2748
                     /locus_tag="VFV55_02505"
     CDS             553..2748
                     /locus_tag="VFV55_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX4943194.1"
                     /translation="MTSNYFRNRTAGAIPAALVALMLALAAPLAWAQGSARNVVESIS
                     FSSIQGGKILVKVGMKEPLAALPQSFAVSNPARIAIDLPDTTSSLGKTQVDAGEGDLK
                     SVSVVQTANRTRLVMNLARSLTYTTALDGRLLVVTIDGSQASASTAPPAAGAVSTVFA
                     EAAPGAVRQNIRDVDFRRGNAGEGRIIVDLSSASTGIDIRQQGRSILIDFINSSVPKN
                     LVRRLDVGDFGTPVRYVDTFEQAGNARMVIEPRGIWEYSAYQTDTQFILEVKPVKEDP
                     NKLVQSSTPGYAGEKLSLNFQNVEVRAVLQVIADFTGLNIITSDTVGGNLTLRLKDVP
                     WDQALDIILQSKGLSKRKNGNVVLIAPTDELAAKEKLALEANAQISDLEPLRTESFAL
                     SYAKAEDLKKLLSDKDQKILSKRGSATVDERTNTLFVQDTGTRLEEARRLVLQLDVPV
                     RQVMIEARIVIADDKWGRQLGARFGTQSAFGSGNRNYGVSGTLVDTVSPLGNNPLSRG
                     SSNLTSPGSGLGSIAGPQGIVPAGGQPEQLNVNLPVVGAAGSLALSILNLGSGNLVNI
                     ELSALEADNRGKVVSSPRVITADKKKAVISQGTEIPYQTAAASGATTIAFKPAVLELA
                     VTPRITPDDRIIMDLEVKKDSVGQIFANIPSIDTKKVTTQVLCDNGDTIVLGGIFEQT
                     TRTTIEKVPLLGDVPFIGNLFKRTVKQDDKTELLIFVTPKIVKDTLTVR"
     gene            2828..3370
                     /locus_tag="VFV55_02510"
     CDS             2828..3370
                     /locus_tag="VFV55_02510"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX4943195.1"
                     /translation="MDPGNVFLVGMMGSGKSTVGRALARRLGREFVDCDREIVERTGV
                     PIATIFEIEGEDGFRRREAAMLAELADRSGTVVATGGGAVLDAGNRRLMREHGIVVYL
                     HTPLDHLHERTRRDVARPLLATGDRRATLTELLVARDPLYREVAHIVVESGTPSAGSL
                     AGRIAELLGAHEGKAQAAPR"
     gene            3367..4449
                     /gene="aroB"
                     /locus_tag="VFV55_02515"
     CDS             3367..4449
                     /gene="aroB"
                     /locus_tag="VFV55_02515"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX4943196.1"
                     /translation="MSARTLTVGLGERSYPIHIGPGLIGQGALFAPYLGSGRAALVTN
                     ETVAPLYADTVEKSLAACGARFFRVVIPDGEAEKRWETLERVYGELLRAQADRRTVIV
                     ALGGGVVGDLAGFAAATYQRGVPLIQVPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFH
                     QPLAVIADTRTLDTLPDRELAAGLAEVIKYGAIRDLPFFEWLEENVDRLRAREAAALA
                     HAIRRSCEIKAEVVALDERESGVRALLNLGHTFGHAIETLEGYGAWLHGEAVAAGMAM
                     AARLSARLGRLPAGDARRLEALIRRAGLPVEPPALAVDAWLDAMGRDKKNESGRITFI
                     LLDRLGAAAIEKHVPAGELEAFLTGR"
     gene            complement(4465..4773)
                     /locus_tag="VFV55_02520"
     CDS             complement(4465..4773)
                     /locus_tag="VFV55_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4943197.1"
                     /translation="MAMIERFEALLAAGKDGALLRFGLGSEYLKAGDAQRAAMHLREA
                     LARDPQYSAAWKLLGKALEGGDRAAAAEAYRRGITAAESKGDKQAAREMQVFLKRLER
                     "
     gene            4890..6470
                     /locus_tag="VFV55_02525"
     CDS             4890..6470
                     /locus_tag="VFV55_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943198.1"
                     /translation="MLRTLRLALAALAILGLPALAPAKEREVSIGLQAAITSMDPHYH
                     NLSPNNSMMLHVFEPLIKRDENQKLVPGLATSWKALDDLTWEFKLRRNVRFHDGSPFT
                     ADDVVFTLKRVPNVPNSPSSFATFTKPIIDVKVVDPYTLVFKTATPHVLLPSDLASVM
                     IVSKVSGEKAATEDYNSGKAAIGTGPYKFAEYVPNQRVVLKANYAYWGGEEPWDKVTF
                     KILTNPAARVAALLSGDVQMIETVPTADIARLAKDPKYSLSDKVSNRVIYVHLNQSTE
                     KAPPFVTDKSGKPLEKNPFRDARVRKALSIGINRDAIVDRIMEKKAVAAAQLLPDFFY
                     GTSKKLKPAKYDPEGAKKLLAEAGYPGGFALTIHGPNNRYINDDKVAQAIAQFYSRIG
                     IDAKVETMPSAVYFTRATKLEFGYMVLGWGTESGEQGSSLRSLLATYDRDKGMGVTNR
                     GRYSNPAFDKLLATALLTMDDKKREALIQQAAETAMNDTALIPLHYEVSTWATSRGYR
                     YTPRTDQYTLATGLRSAK"
     gene            6557..7540
                     /locus_tag="VFV55_02530"
     CDS             6557..7540
                     /locus_tag="VFV55_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4943199.1"
                     /translation="MLAYAIRRFGQSLFVLVLMSFLVFVGVYAIGNPVELLVNPQADE
                     AERIRATAALGLDMPFLEQYWVFLKGAASGNLGKSFVYNVPALSLILDKLPATIELAF
                     VAMVIAIVLGIPLGLVAGLKPDSLVGRGIMAGSILGFSLPTFWVGLVLIMVFSVQLGW
                     LPSNGRGETLAFAGIPVSFLTADGLAHLAMPAVNLALFKLSLLIRLTRAGTREAVLQD
                     YVKFARAKGLTQGRVIGVHVLKNILIPIVTVIGLELGSVIAFAIVTESIFAWPGTGKL
                     LIDSINQLDRPVIVAYLLVIVTLFIVINFVVDVLYSILDPRVRLGGSAAAH"
     gene            7544..8485
                     /locus_tag="VFV55_02535"
     CDS             7544..8485
                     /locus_tag="VFV55_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4943200.1"
                     /translation="MIDAGDSPAQAKVAETLLTSKELATGPWSRFRADFAESRLAMLG
                     LALLVVILLIGILAPVISPQNPYDLAQLDVMDSKLAPGEESANGRTYWLGTDDQGRDM
                     LSGIFYGLRISLGVGVASTVFALSIGLTFGLLAAYFGGWVDMLIMRIVDIQLSFPAIL
                     IALVLLAVLGQGVDKIVVALVTVQWAYYARTVRSSALVERQKEYIEAARVLALGQARV
                     VFRHLLPNCLPPLIVVATVQVAHAIALEATLSFLGLGLPITEPSLGLLISNGFQYLLS
                     GKYWISFYPGIALLLTIMSINLVADQLRDILNPRLRR"
     gene            8482..9450
                     /locus_tag="VFV55_02540"
     CDS             8482..9450
                     /locus_tag="VFV55_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4943201.1"
                     /translation="MSGAPLLQVDGLCMHFFTKAGVVKAVDDVSFSVGRGEILGLVGE
                     SGSGKSMTGYSIIGLIDPPGRIVEGRIVFDGIDLTRLDAAAWRRIRGNRVAMIFQDPM
                     MTLNPVLRIDTQIVEAVRAHADVSRESALARARESLVRVGIASPDERLRAYPHQFSGG
                     MRQRVAIAIAMVNSPDLIIADEPTTALDVTIQGQILFEMQKVCRESGTALIWITHDLS
                     VIAGLADKVCVMYAGRFVESGSVAEILDRPLHPYTRGLIDSVPSRNERGRPLRQIPGM
                     TPSLLSLGPGCPFRERCGFATALCAESPPITEPAPGRRLRCFHPAT"
BASE COUNT         1598 a         3402 c         2992 g         1539 t
ORIGIN      
        1 ccaaggacaa ggcggccaag ggggctaaga aatgaggcgc tggctgctgc tgtgcgccgc
       61 ggcactcatg ctcgtctcgt gcagttccga gatcgacgag ctcaggcagt tcgtgaagaa
      121 ctcggaaaag agcctgccgc gaaagatcga cccgctcccg gccgtcaagc cgttcgagcc
      181 tttcacctac gagggcttcg acctgcccga ccccttcaag ccgcgcaaga tcacggccag
      241 ggaaggcgcc gctggcggcg tcgctcccga cctcaaccgg cgcaaggaac ccctcgaggc
      301 ctaccccctc gagcagctga agatggtggg cacgctgcag caaggcaagc agatgttcgc
      361 catcgtccgg gcggaaaaga ccctgtatcg cgtgaagaag ggcaactaca tggggcagaa
      421 cttcggcctc atcaccgaca tcacggatgc cgacatcaag ctgaaggaaa tcgtccagga
      481 cagcgccggg gactgggcag aacggcagag tgtcctgccg cttctcgaag agactggcaa
      541 aggggataga aagtgacctc caactacttc cgcaaccgaa ccgccggcgc gattccggcc
      601 gcactcgtgg cgttgatgct cgcgcttgcc gccccgctcg cctgggcgca aggctcggcc
      661 aggaacgtgg tcgagtcgat cagcttctcc tcgatccagg gcggcaagat cctggtcaag
      721 gtgggcatga aggagccgct cgcggccctt ccccagagct tcgcggtcag caatcccgcg
      781 cgcatcgcga tcgaccttcc ggacaccacg tccagcctcg gcaagacgca ggtcgacgcg
      841 ggcgagggcg acctcaagag cgtctccgtg gtccagacgg ccaaccgcac gcggctggtg
      901 atgaatctcg cgcgcagcct cacctacacc accgcgctcg acggcaggct cctcgtcgtc
      961 acgatcgacg gttcgcaggc ctccgcgtcc acggccccgc ccgccgccgg cgccgtctcc
     1021 accgtgttcg ccgaagcggc gccgggcgcc gtccggcaga acatccgcga cgtcgacttc
     1081 cgccgcggca atgccggcga gggtcgcatc atcgtggacc tctcctccgc cagcaccgga
     1141 atcgacatcc ggcagcaggg ccgttcgatc ctcatcgact tcatcaacag ttccgttccg
     1201 aagaacctcg tgcgccggct ggacgtgggc gacttcggca cgccggtgcg ctacgtcgat
     1261 accttcgagc aggccggcaa cgcgcgcatg gtgatcgagc cgcgcggcat ctgggaatac
     1321 tcggcctacc agaccgacac ccagttcatc ctcgaggtga agccggtcaa ggaagacccg
     1381 aacaagctgg tgcagagctc gacacccgga tacgcgggcg agaagctttc cctcaacttc
     1441 cagaatgtcg aggtgcgtgc ggtccttcag gtgatcgccg acttcaccgg cctcaacatc
     1501 atcaccagcg atacggtcgg cgggaacctc acgctgcgcc tgaaggacgt tccctgggac
     1561 caggctctcg acatcatcct gcagtccaag ggcctgtcga agcgcaagaa cggcaacgtg
     1621 gtgctgatcg cgccgaccga cgaactggcc gccaaggaaa agctcgccct cgaggcgaat
     1681 gcccagattt ccgacctgga gccgcttcgc accgagtcct ttgcgctgtc gtacgccaag
     1741 gccgaggacc tgaagaagct gctctccgac aaggaccaga agatcctgtc caagcgcgga
     1801 agcgccaccg tcgacgagcg caccaacacc ctgttcgtcc aggacacggg cacgcgcctc
     1861 gaggaggcgc gccggctggt cctgcagctc gacgtcccgg tgcgccaggt gatgatcgag
     1921 gcgcgcatcg tcatcgccga cgacaagtgg ggccgccagc tcggcgcccg cttcggcacc
     1981 cagtccgcgt tcggctcggg caaccgcaac tacggcgtgt cgggcaccct ggttgatacg
     2041 gtgtcgccgc tcggtaacaa ccccctgtcc cgcggctcgt cgaacctgac gtcacccggc
     2101 tcgggcctcg gctccatcgc cggcccgcag ggaatcgtgc ccgccggagg ccagcccgag
     2161 cagctcaacg tcaaccttcc ggtcgtcggt gccgccggct cgctggcgct gtcgatccta
     2221 aacctcggca gcggcaatct cgtgaacatc gagctttccg ccctcgaggc cgacaaccgc
     2281 ggcaaggtgg tgtcgagccc ccgcgtgatc accgccgaca agaagaaggc cgtcatctcc
     2341 caggggacgg agatcccgta ccagacggcg gcggcaagcg gcgcgacgac gatcgccttc
     2401 aagccggcgg tgctggaact cgccgtgacc ccgcgaatca ctcccgacga ccgcatcatc
     2461 atggacctcg aggtgaagaa ggactccgtg ggccagattt tcgccaacat cccctcgatc
     2521 gacacgaaga aggtgacgac ccaggttctc tgcgacaacg gcgatacgat cgtcctgggc
     2581 gggatcttcg agcagacgac ccgcacgacg atagaaaagg tcccgctgct gggcgacgtc
     2641 ccgttcatcg gcaacctttt caaacgcacc gtgaagcagg acgacaagac ggagctcctg
     2701 atcttcgtga ccccgaagat cgtcaaggac acgctcacgg tccggtgacg ctgccctcac
     2761 cccgatcgag ccccggccac gccggggctt gtcgtttctg cgctatcctt tccgcaaggg
     2821 cgatcgaatg gatccgggca acgtcttcct cgtcggcatg atgggctcgg gcaagagcac
     2881 cgtcggcagg gcccttgcgc gccgactcgg gcgcgagttc gtcgactgcg atcgcgagat
     2941 cgtcgagcgc accggagtgc ccatcgccac gatcttcgag atcgaaggcg aggacgggtt
     3001 caggcgccgg gaggcggcga tgctcgcgga actcgccgac cggagcggca cggtggtcgc
     3061 caccggtggc ggcgcggtgc tcgacgccgg gaaccgccgc ctgatgcgcg agcacggcat
     3121 cgtggtctat ctccacacgc ccctcgacca cctgcacgag cgcacgcgcc gcgacgtcgc
     3181 gcgaccgctc ctcgccaccg gcgaccggcg cgccaccctc accgagcttc tcgtggcccg
     3241 cgaccccctg taccgggagg tcgcacacat cgtcgtcgag agcggaactc ccagcgccgg
     3301 gtcgctggcc gggcggatcg cggaacttct cggggcgcat gaggggaagg cgcaggcggc
     3361 gccccgatga gcgcgcgcac actcacggtc ggcctcggcg agcgcagtta ccccatccac
     3421 atcgggccgg gcctgatcgg ccagggcgcc ctcttcgcgc cgtacctggg atcggggcgg
     3481 gcggcattgg tcaccaacga aacggtcgcg cccctctacg ccgacacggt ggagaaatcc
     3541 ctggccgcgt gcggtgcccg tttcttccgg gtggtcattc ccgacggcga ggccgagaag
     3601 cgctgggaaa ccctggagcg ggtatacggg gagctgcttc gggcccaggc agaccggcgc
     3661 acggtcatcg tggcgctcgg cggtggcgtg gtcggcgacc tggccgggtt cgccgcggcg
     3721 acctaccagc ggggcgttcc gctgattcag gtgccgacga cgctactggc ccaggtggac
     3781 tcgtccgtcg gcggcaagac cgcggtgaac catccgctcg gcaagaacat gatcggcgcc
     3841 ttccaccagc cgctcgccgt gatcgccgac accaggacgc tcgacacgct gcccgaccgg
     3901 gaactcgcgg cggggctggc cgaggtgatc aagtacgggg cgatccgcga cctgcctttc
     3961 ttcgaatggc tcgaggaaaa cgtcgatcgc ctgcgcgcgc gcgaagcggc cgcgcttgcg
     4021 cacgccatcc gacgttcgtg cgagatcaag gcggaggtcg tcgcgctgga cgagcgcgaa
     4081 tcgggcgtgc gcgcgctgct taacctcggc cacacgttcg ggcatgccat cgagaccctc
     4141 gaaggatacg gcgcatggct gcacggcgag gccgtggcgg cgggcatggc gatggccgcg
     4201 cggctttcgg cacgcctcgg ccggcttccc gccggggacg cgcgtcgcct cgaggcgctg
     4261 atccggcgcg ccggcctgcc cgtcgagccg cccgcgctcg cggtcgatgc ctggctcgat
     4321 gccatggggc gcgacaagaa gaacgagtcg ggccgcatca ctttcatcct gctcgatcgc
     4381 ctgggcgctg ccgccatcga gaagcacgtg ccggcaggcg agctggaggc cttccttacc
     4441 gggcgctagg cgcgatgctc tgtgctagcg ctcgagccgc ttcaggaaca cctgcatctc
     4501 ccgcgcggcc tgcttgtcgc ccttcgattc cgctgccgtg atgccgcgcc gataagcctc
     4561 tgcggccgcc gcgcggtcgc cgccctcgag ggccttgccc agcagcttcc atgccgccga
     4621 gtattgcgga tcccttgcga gcgcctcgcg aagatgcatc gccgcgcgtt gcgcatcgcc
     4681 ggccttgagg tattccgaac ccagcccgaa gcgaaggagc gcgccgtcct tgcctgccgc
     4741 gagcagtgcc tcgaagcgct cgatcatcgc catgttccaa gtctccgaac cgccgcccgg
     4801 tcccggggaa tgacggtatc atcggaaccg ccgcatttcc cgctttccgg aatcccatcg
     4861 tcccgacgaa aaccagagga gttcccgtca tgctgcgcac cctgcgactt gcgcttgccg
     4921 cactcgccat cctgggcctg ccggccctgg cccccgccaa ggaacgcgag gtatcgatcg
     4981 gcctgcaggc cgccatcacc tcgatggacc cgcactacca caacctgtcg ccgaacaact
     5041 cgatgatgct gcatgtattc gagcccctca tcaagcgcga cgagaaccag aagctggtcc
     5101 ccggcctggc gacctcgtgg aaggcactgg acgacctcac ctgggagttc aagctgcgca
     5161 ggaacgtgcg cttccacgac ggctcgccct tcacggccga cgacgtggtg ttcacgctca
     5221 agcgcgtgcc caacgtcccc aacagcccgt cttccttcgc gaccttcacc aagccgatca
     5281 tcgacgtgaa ggtggtcgat ccgtacaccc tcgtcttcaa gaccgccacc cctcacgtcc
     5341 tgctgccgag cgacctcgcc tcggtcatga tcgtctcgaa ggtgagcggc gaaaaggccg
     5401 ccaccgagga ctacaactcc ggcaaggcgg cgatcggcac cgggccctac aagttcgcgg
     5461 agtacgtgcc caaccagcgc gtcgtgctca aggccaacta cgcctactgg ggaggcgagg
     5521 agccgtggga caaggtcacg ttcaagatcc tcaccaaccc cgccgcccga gtggcggcct
     5581 tgctgtccgg cgacgtgcag atgatcgaga cggttcccac cgcggacatc gcgaggctcg
     5641 ccaaggaccc gaagtacagc ctctccgaca aggtgtccaa ccgggtgatc tacgtgcacc
     5701 tcaaccagag cacggaaaag gcgccgccct tcgtgacgga caagtccggc aagccgctgg
     5761 agaagaaccc cttccgcgac gcgcgcgtgc gcaaggccct gtcgatcggg atcaaccgcg
     5821 acgcgatcgt cgaccgcatc atggagaaga aggcggtcgc cgcggcgcag ctcctgcccg
     5881 atttcttcta cggcacgagc aagaagctca agcccgcgaa atacgacccc gagggcgcga
     5941 agaagctgct cgccgaggcg ggataccccg gcggcttcgc cctcaccatc cacggcccca
     6001 acaaccgcta catcaacgac gacaaggtcg cccaggccat cgcccagttc tactcccgca
     6061 tcggcatcga cgcgaaggtg gagacgatgc cttcggccgt gtacttcacc cgcgcgacga
     6121 agctcgagtt cggctacatg gtgctcggct gggggacgga atccggcgag cagggctcca
     6181 gcctgcgctc tctcctcgcg acctacgacc gcgacaaggg catgggcgtg accaaccgcg
     6241 gccgttattc caatcccgcg ttcgacaagt tgcttgccac cgcgctgctc accatggacg
     6301 acaagaagcg cgaggcgctg atccagcagg ccgccgagac ggcgatgaac gacacggccc
     6361 tcatcccgct gcactacgag gtgagcacct gggccacctc caggggctac cgctacaccc
     6421 cgcgcacgga ccagtacacc ctcgcgacgg gactgcgttc cgcgaaatag ggtcccacca
     6481 cggcaagccg ggccgcgcct gcgcgcggcc cggcgtttcc gaggcgtaag atagcccgtc
     6541 cccgcgagcc ttcaccatgc tggcctacgc catccgccga ttcgggcaga gcctcttcgt
     6601 gcttgtcctg atgtcgttcc tcgtgttcgt cggggtgtac gccatcggca atccggtgga
     6661 gctgctcgtc aacccgcagg ccgacgaggc cgagcgcatc cgcgcgaccg ccgcgctggg
     6721 cctggacatg cccttcctcg agcagtactg ggtgttcctc aagggcgcgg cgagcggaaa
     6781 cctcggcaag tccttcgtct acaacgtgcc ggccctttcg ctcatcctcg acaagctccc
     6841 ggcgaccatc gagctcgcgt tcgtggcgat ggtgatcgcc atcgtgctcg ggattccgct
     6901 cggcctggtc gccggcctca agcccgactc cctcgtcggg cgcggcatca tggcgggctc
     6961 catcctcggc ttcagcctgc ccaccttctg ggtcgggctg gtcctcatca tggtcttctc
     7021 ggtccagctg ggctggctgc cctccaacgg gcggggcgag accctggcgt tcgccggcat
     7081 cccggtgagc ttcctcacgg ccgacgggct tgcccacctg gccatgccgg cggtgaacct
     7141 cgcgctcttc aagctttcgc tcctcatccg gctcacgcgt gccggcacgc gcgaggcggt
     7201 gctgcaggat tacgtgaagt tcgcccgcgc caagggcctc acgcagggcc gcgtgatcgg
     7261 cgtgcacgtc ctgaagaaca tcctcattcc gatcgtcacc gtgatcggcc tggagctggg
     7321 ctcggtgatc gccttcgcca tcgtgaccga gtcgatcttc gcgtggcccg gcaccggcaa
     7381 gctcctcatc gactcgatca accagctcga ccgtccagtg atcgtggcct acctcctggt
     7441 gatcgtgacg ctcttcatcg tcatcaattt cgtggtcgac gtcctctact cgatcctgga
     7501 tccgcgcgtg cggctgggcg gctcggcggc ggcccactga ccgatgatcg atgccggcga
     7561 ctccccagcg caagccaagg tagcggaaac gctgctgacg tcgaaggagc ttgccaccgg
     7621 cccgtggtcg cgcttccgcg ccgacttcgc cgaaagccgg ctcgccatgc tcggcctcgc
     7681 actcctcgtg gtcatcctgc tcatcggcat tctcgcgccg gtgatatcgc cgcagaatcc
     7741 ctatgacctc gcgcaactcg acgtcatgga ctcgaagctc gcccccggcg aggaatcggc
     7801 gaacggcagg acgtactggc tgggcacgga cgaccagggc cgcgacatgc tgtcggggat
     7861 cttctacggc ctgcgcatct cgctcggcgt gggcgtggcg agcacggtgt tcgcgctttc
     7921 gatcggcctt accttcggcc tcctggcagc ctacttcggc ggatgggtcg acatgctgat
     7981 catgcgcatc gtcgacatcc agctttcgtt tccggcgatc ctcatcgcgc tcgtcctcct
     8041 tgccgtgctc ggccaggggg tggacaagat cgtggtcgcg ctggtgactg tccagtgggc
     8101 ttactacgcg cgcacggtgc gctcttcggc tctcgtggag cggcagaagg aatacatcga
     8161 ggccgcccgc gtgctcgccc tcggccaggc acgggtcgtg ttccggcacc tgctgcccaa
     8221 ctgcctgccg cccctcatcg tggtggccac ggtgcaggtt gcccatgcca tcgccctgga
     8281 ggccacgctc tcgttcctgg gcctgggact tccgatcacc gagccgtcgc tggggctgct
     8341 catctcgaac gggttccagt atctcctgtc gggcaagtac tggatcagct tctatcccgg
     8401 catcgcgctc ctgctcacga tcatgtcgat caatctcgtg gccgaccagc tgcgcgacat
     8461 cctcaacccg agattgcgcc ggtgagcggt gcaccgctgc tgcaggtcga cggcctgtgc
     8521 atgcacttct tcaccaaggc cggcgtggtg aaggcggtcg acgacgtgag cttcagcgtc
     8581 ggccgcggcg agatcctggg cctggtcggc gaatcggggt cgggcaagtc gatgacgggc
     8641 tactcgatca tcggcctcat cgacccgccg ggccggatcg tcgagggccg catcgtcttc
     8701 gacggcatcg acctcacgcg gctcgacgcc gcggcctggc ggcggatccg gggaaaccgc
     8761 gtcgccatga tcttccagga cccgatgatg accctcaatc cggtcctgcg gatcgacacg
     8821 cagatcgtcg aggcggtgcg ggcgcacgcc gacgtgtcgc gcgaaagcgc gctggcccgc
     8881 gcgcgcgaat ccctcgtgcg ggtgggcatc gcctcgcccg acgagcgcct gcgcgcctat
     8941 ccgcaccagt tctccggcgg catgcgccag cgcgtggcga tcgccatcgc gatggtcaac
     9001 tcccccgacc tcatcatcgc cgacgaaccc acgaccgcac tggacgtcac catccaggga
     9061 caaatcctct tcgagatgca gaaggtctgc cgggaaagcg gcacggcgct catctggatt
     9121 acccacgacc tgtcggtgat cgcgggattg gccgacaagg tgtgcgtcat gtacgcgggg
     9181 cgcttcgtcg agtccggctc cgtcgccgag atcctcgacc ggcccctgca tccctacacc
     9241 cgcggcctca tcgactcggt gccgagccgc aacgagcgcg ggcgccccct tcgccagatt
     9301 ccgggcatga cgccgtcgct gctctccctc gggccgggat gtccgttccg ggagcgctgc
     9361 ggctttgcca cggcactctg cgccgagagc ccgcccatca ccgaacccgc gcctggccga
     9421 aggctgcgct gctttcatcc ggccacatga tcgccaccgt ggacgccctg cctccgctcc
     9481 tcgcgcttcg cgggatctcg aagcgattct ccaatcggct cgatctcgcc t
//
LOCUS       DASUEV010000081         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_711146, whole genome shotgun sequence.
ACCESSION   DASUEV010000081 DASUEV010000000
VERSION     DASUEV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711146"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="VFV55_02545"
     CDS             complement(<1..1874)
                     /locus_tag="VFV55_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HEX4943202.1"
                     /translation="MAGKAGGYRGGIDAALADFIELLGKAILSGIAASVILALLALGL
                     ATTAGAQTRPNDAQTGTLLFRTGAAGVFAPAPKVETDVTIHVTGMVARTRVSQTFHNP
                     GTDWVEGVYVFPLPENAAVDHLWLRIGEREIEGQVKEKEEARRVYAQAKREGKKAALV
                     EQQRPNLFTNAVANIGPGEYVRVLIEYQQTLAIDNGEYRLRFPLAITPRYEPAGAAIP
                     DQPGTDGPPKGALSGSPASDPGIDPILHPDYAPAGHRGVNPVAIGVVIDYGMPLASVT
                     SSYHDAWIEKESGTRTVVYLKKEQEEADRDFELVWSPQGGPDPLAAVFTESRNGTDYT
                     LLMVMPPQPTAAEKAAIVPLPRETVFIIDTSGSMQGTSIRQAKEALLTGLGTLTARDR
                     FNVVEFNSATRPMWPDALPATIDNVKFASEWVAKLKADGGTEMAGALTFALNGRDTPG
                     YLRQVVFMTDGAVGNEDQLFKLIAARLGATRLFTVGIGSAPNSHFMTKAAQFGRGTFT
                     YIGDLREVQEKMAKLFAKIESPVLKDVSIRWPDGTPVETFPARVPDLYLGEPIVVAAA
                     HPALKGTIVVSGTRGIQPWSVALTPSPSGDPTGVGALWARAKIASLMDELRLGADEAV
                     "
BASE COUNT          319 a          679 c          733 g          357 t
ORIGIN      
        1 atcaccgcct cgtcggcgcc caggcgcagc tcgtccatca ggcttgcgat cttcgccctg
       61 gcccagaggg cgcccacgcc ggtcggatcg ccgctcggcg aaggcgtgag cgccacgctc
      121 cagggttgga tgccgcgcgt cccggagacg acgatcgtcc ccttgagcgc cggatgggcg
      181 gccgccacga cgatcggctc gccgaggtag agatcgggca cgcgcgccgg gaaggtctcg
      241 acgggtgtgc cgtcgggcca gcggatcgac acgtccttga gcaccggcga ctcgatcttc
      301 gcgaagagct tcgccatctt ctcctgcacc tcgcgcaggt cgccgatgta ggtgaaggtg
      361 ccgcggccga actgcgccgc cttggtcatg aagtggctgt tgggcgcgga gccgatgccc
      421 acggtgaaga ggcgcgtggc gcccaggcgt gcggcgatga gcttgaagag ctggtcctcg
      481 ttgccgaccg cgccgtcggt catgaagacc acctggcgaa ggtagccggg cgtgtcgcgg
      541 ccattcagcg cgaacgtgag ggcgccggcc atttcggtgc cgccgtcggc cttgagcttc
      601 gcgacccact cgctggcgaa cttcacgtta tcgatcgtcg cgggcagcgc gtcgggccac
      661 atcggtcgcg tcgccgaatt gaattccacg acattgaaac ggtcgcgcgc cgtgagggtg
      721 ccgaggcccg tgaggagagc ctccttcgcc tgccggatcg aggtgccctg catggacccg
      781 gaggtgtcga tgatgaacac ggtctcgcgc ggcagcggca cgatggcggc cttctccgcg
      841 gccgtcggct gcggcggcat caccatgaga agcgtgtaat cggtgccgtt cctcgattcc
      901 gtgaagacgg cggccagcgg gtccgggcct ccctgcggcg accacaccag ctcgaagtcc
      961 cggtcggctt cctcctgttc cttcttcagg tagacgacgg ttcgcgtgcc ggactccttc
     1021 tcgatccacg cgtcgtggta ggagctggtc acactggcga gcggcatgcc gtagtcgatc
     1081 accacgccga tggccaccgg gttcacgccg cggtggccgg cgggcgcgta gtcgggatgc
     1141 aggatggggt cgatgccggg gtcgctggcc gggctgccag agagggcccc cttgggcggc
     1201 ccatccgtac cgggttgatc gggaatcgcg gcgcccgcgg gctcgtagcg cggcgttatc
     1261 gcgagcggga agcgcaggcg gtactcgccg ttgtcgatgg ccagcgtctg ctggtactcg
     1321 atgagcacgc gcacgtactc gcccggcccg atgttggcga cggcgttggt gaagaggttg
     1381 gggcgctgct gttcgaccag cgcggccttc ttcccctcgc gcttcgcctg agcgtaaacg
     1441 cgccgcgcct cctccttctc cttcacctgg ccctcgatct cgcgctcgcc gatgcgcagc
     1501 cacaggtggt cgacggcggc gttctcggga agcggaaaga cgtacacgcc ctcgacccag
     1561 tcggtgccgg ggttgtggaa ggtctgggat acgcgcgtgc gggcgaccat gccggtcacg
     1621 tgaatcgtga cgtccgtctc gaccttcggc gccggggcga aaacgcccgc ggcgccggtg
     1681 cggaacagga gggttcccgt ctgcgcgtcg ttgggcctgg tctgcgcgcc cgcggtcgtc
     1741 gcaagcccca gggctaagag ggcaaggatc acgcttgcgg cgatgcccga gaggattgcc
     1801 ttgcccagca actcgatgaa gtcggccagt gccgcatcga ttccgccgcg gtatccgcct
     1861 gccttgcctg ccatgttccc tccccctgtg cgccgaccca ttcggcaacg ccattacgcg
     1921 cgggcggggg ggcgccatcc ttgggcaaag cgggaagggt ggcgaccaat tcgggcaaga
     1981 aaatgacaac gaaaaggggt caggttactt tgcacaaaaa ataggcaaaa cggaaaaggg
     2041 gtcaggttac ttttcagccc gccgtcttgc gacggcaata cgatagaa
//
LOCUS       DASUEV010000082         8516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_482368, whole genome shotgun sequence.
ACCESSION   DASUEV010000082 DASUEV010000000
VERSION     DASUEV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482368"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1546)
                     /locus_tag="VFV55_02550"
     CDS             complement(44..1546)
                     /locus_tag="VFV55_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4943203.1"
                     /translation="MIPRRIAAAALLVALAAATTLVAVAEPLRVGSKRFTESYILGEI
                     LVQAAQRAGAAAEHRPGLGNTAILFEALQRGAIDAYPEYTGTIAREILKDGGHADLAA
                     INRRLAPMGLAASVPFGFSNGYALGMRESEAAAKGIATLSDLARHPEIALGLSHEFLG
                     REDGWPGLRAAYGMAQKDPRGLDHGIAYEALAAGQVAVIDLYTTDAKIARLGVRVLAD
                     DRAFFPRYDAVVLHRLDAPGRHPRAFAAWSRLQGRIDEAAMIRLNGRAELDRVDFAAV
                     AGEFLGSTTIAAPRSLWSAVFAPDFARLAFEHAMLVFGSLAVAVAAGVPMGIVAAKVR
                     VFAQPVLVAAGLVQTIPSLALLAFLIPLTGIGAWPALIALFLYALLPIARNTHAGLME
                     VAGGLRHAALALGLTPRQTLVRIELPLALPTILAGVKTSAVVNVGTATIAAFVGAGGF
                     GERIAQGLAVNDHQLLLAGALPAAALALAVHAFFELVERWLRRGTHSAAR"
     gene            complement(1593..2906)
                     /locus_tag="VFV55_02555"
     CDS             complement(1593..2906)
                     /locus_tag="VFV55_02555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX4943204.1"
                     /translation="MTTLLARRTARLWIALASFVPAVALALAPLPVAKPEAVGMSSQK
                     LALIGEAMRKEVADGSFRGAVVMVARKGKLVYHEAFGMQTPTTKMSEGSIFRIYSMTK
                     PLVSVAAMMLVEEGKVQLTDPVGKYLAGFDKLQVSVAGKDASGNVAYTLAAADRPMTV
                     QDLLRHTAGLAYGEITTNAPVKEGLEKAGVYRKDLDYEARGVTPAEQVERMATVPLAF
                     QPGTTWHYSLGVDILGRVVEAASGMRLADFLEARLFKPLKMVDSGFWVPPAKMPRLAE
                     TLEKDRFANRGFPLIDVSSVPKNDSGGAGGVSTASDYLRFSQMLMNGGVLEGARVLSP
                     TTVRLMTSDHLGSFIATPVQPGELLLGTKGYTFGLGFTVRQGDGVAGVAGSAGEFMWA
                     GYAGTFFWVDPREQVTAVLMTQAASPNRAYFRRMIKNLVYQAITN"
     gene            3039..5282
                     /locus_tag="VFV55_02560"
     CDS             3039..5282
                     /locus_tag="VFV55_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX4943205.1"
                     /translation="MAPSPGAAHQPYIAASDHVAEFTVRAVLMGTLLGIVFGASSLYL
                     VLKVGLTVSASIPVAVIAITLFRLVAKGGGRDSTILENNIVQTAGSAGESIAFGLGVT
                     MPAILILGFDLEIVRVMLVGVLGGALGILMMIPLRLTLIVARHGELKYPEGTACAEVL
                     KAAEAAKASRGSRGTGAADDASRAGVIFLGFAIGLAYKAANVALKGWKDVPEKVFDAP
                     LKGGSVGVEISPELLGVGYIIGPRIASIMCAGGVLAYLLLIPMISFFGDALQVPVAPG
                     VKLIHDMDPGEIRGAYVLYIGAGAVAAGGIVSLVRALPTIWRSLREGLKGFGEAGDAS
                     TRRTDRDIPMKWVLVGALAIIVAITLAPPLHMNLLGAMLILVFGFLFSTVSSQLTGEI
                     GSSSNPISGMTVATLLLTCLIFLLVGWTGGPYYVTALSVGAIVCIASSNAGTTSQDLK
                     TGFLVGATPRLQQFAILAGALASALVLGPILLRLNDAATVYVPIAQVAPGLATDAQSL
                     TERATLQGPQAAQDGGTYRVWHKTDLAGGPAGKYLVDDAGRAVYLVDPGINGSHGTRP
                     DGTEVRKYDAPKAVLMSYIIKGILDRKLPWALVIFGVVIALVLEMSGIASLPFAVGVY
                     LPLSSSAPIFIGGAVRWLVDRRRNRQAQYRDASEAVRSAAGDRSPGVLLASGYIAGGA
                     LAGIVIAFTAGVLSGFDKAVTDWAAANNPFFAGPWADLLSLVPFLGLAVLLHAVGHEK
                     PPRESGA"
     gene            5397..8057
                     /locus_tag="VFV55_02565"
     CDS             5397..8057
                     /locus_tag="VFV55_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX4943206.1"
                     /translation="MNIVNLSIPEVLASLKTSEGGLDAGEAARRLREFGHNRIEEVKG
                     KPLWLRFLREFTHFFALILWVAAALAFFAESRSRGEGMWQLGAAILAVIVINGVFSFW
                     QEYRAERAISALRQLLSPQAKVLRGGLLQEMAAELLVPGDVVLLGEGDNVPADCRLIE
                     ASRLRVNLSTLTGEPLAKPRTAEGIADGRALDAKNLVLAGTSVISGECRAVVFATGMR
                     TEFGRIAHLTQTAGEAGSPLQREIARLSKLVAVLASVLGLTFFGIGTAIGLPFWDNVM
                     FAIGIIVANVPEGLLPTVTLSLAMATQRMARRNALVRHLPSVETLGSATVICCDKTGT
                     LTQNRMSVKQVYAARLQGPTNDLRSIAGDIHLQRNARYCHSLKRGSRNGKQAWLGDPM
                     EIALAEFAGADNAQDDGAVTGDIPFDSERRRMSVVVERDGERWLYCKGAPEVVLLLCD
                     RIDDGDTTVPLDEEGRRRFTEAQDAMAQGGQRVLAFACRKLDADEGPEERNLALSGLI
                     GLHDPPRPEVHEAVSRCRTAGIKVIMVTGDHPDTAVAIAREIGLVRSASPAVVQGGAL
                     LKMTPAQLQIALDSPEILFTRVTAEQKMLVVEGLKAKGHVVAVTGDGVNDAPALKTAH
                     IGIAMGISGTDVAKEAADMILLDDNFASIVNAIEEGRAVFENIRRFLTYILTSNIPEL
                     VPYIAFVIFRIPLPLTIIQILAVDLGTDMLPALALGAERPNRLLMQRPPRPAGERLLT
                     WPLLARAYLWLGVLQAAVSMAAFFFVLQRGGWDYGDMPGKSDPLYLQATTACLAAIVV
                     AQVVNVFVCRHPHESILRFRPADNPLLLAGIAFEVLLILAIVYTPVGNGAFGTLPLSW
                     EPWLLMITLALGIGLLEELRKLLAGAGRRA"
     gene            complement(8086..>8516)
                     /locus_tag="VFV55_02570"
     CDS             complement(8086..>8516)
                     /locus_tag="VFV55_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4943207.1"
                     /translation="FNCVRLAVPHLRRSGNASLMNVSSLAGRLGFALRTPYASAKWGV
                     VGLTRSLAVELGPDNIRVNALLPGVIAGERQARVLAAKAEARGISFEEMESIAFSYSS
                     LKEYVTPQQLADLVAFVASPRGRMISGQALSVCGDTNMLG"
BASE COUNT         1299 a         2998 c         2877 g         1342 t
ORIGIN      
        1 ccccggctcg tcccggccca cccccctcct ccccatcgcc gggctaacgt gccgcggaat
       61 gcgtgcctcg ccgcagccac cgctccacca gctcgaagaa tgcgtgcacg gcgagcgcca
      121 gcgccgccgc gggcagcgcc ccggccagca gcaactggtg atcgttcact gcgaggcctt
      181 gcgctatgcg ctcgccgaag ccccccgccc ctacgaacgc cgcgatggtg gcggtgccga
      241 cgttcaccac ggccgaggtc ttcacgccgg cgaggatcgt gggcagagcc agcggaagct
      301 cgatgcgcac gagcgtttgc cgcggcgtga gccccagcgc gagtgcggca tgccgaagcc
      361 cgcccgcgac ttccatgagt cccgcgtgcg tattgcgcgc gatgggcagg agcgcgtaga
      421 ggaacaatgc gatgagcgcc ggccacgcgc cgatgccggt gagcggaatg aggaaagcca
      481 gcagcgccag cgagggaatg gtctgcacca gccccgccgc gaccagcacg ggctgcgcga
      541 agacgcgcac cttcgccgcg acgatgccca tcggcacgcc cgccgcgacg gccacggcga
      601 gcgaaccgaa cacgagcatc gcgtgttcga aggcaaggcg cgcgaagtcc ggtgcgaaca
      661 ccgcggacca gaggctgcgc ggcgcggcaa tcgtggtcga gccgaggaac tcgccggcca
      721 ccgctgcgaa atcgacgcga tccagctcgg cgcgcccgtt gaggcggatc atcgcggctt
      781 cgtcgatcct gccctgcaag cgcgaccacg cggcgaacgc gcgcggatgt cgccccggcg
      841 cgtcgagacg gtgcagcacc acggcgtcgt agcgcgggaa aaaggcgcgg tcgtccgcga
      901 gcacccggac gcccaggcgc gcgatcttgg cgtcggtggt gtagaggtcg atcaccgcga
      961 cctgcccggc ggccagggcc tcgtaggcga tcccgtgatc gaggccgcgc ggatccttct
     1021 gcgccattcc gtacgcggca cgcagccccg gccaaccatc ctcgcgcccg aggaattcgt
     1081 gcgacaggcc gagcgcgatc tccgggtggc gcgccaggtc cgagagcgtc gcgatgccct
     1141 ttgcagccgc ctcggattcg cgcatgccca gcgcgtagcc gttggagaac ccgaagggca
     1201 cgctcgccgc caggcccatg ggcgcaaggc gccggttgat ggccgcaagg tccgcgtggc
     1261 cgccgtcctt gaggatctcg cgcgcgatcg tcccggtgta ctcgggatag gcgtcgatgg
     1321 ctccgcgctg cagggcctcg aagaggatgg cggtgttgcc caggcccggc cgatgctcgg
     1381 ccgccgcccc ggcgcgctgc gcggcctgca cgaggatttc gccgaggatg tacgactcgg
     1441 tgaaccgctt cgagccgacg cgcaggggct cggcgaccgc cacgagcgtt gtcgccgcgg
     1501 cgagggcgac gaggagggcg gcggctgcga tgcggcgcgg gatcatgggg gctcgcgaaa
     1561 cggggttcgc atccgcttcc gcccccatcg cctcagttcg tgatggcctg gtagacgagg
     1621 ttcttgatca tgcggcggaa gtaggcgcgg ttcgggctcg cggcctgcgt catgagcacc
     1681 gccgtcactt gctccctggg atcgacccag aagaatgtgc ccgcgtagcc ggcccacatg
     1741 aactcgcccg cggatcccgc cacgcccgcg acgccgtcgc cctgccgaac ggtgaagccg
     1801 agcccgaacg tgtagccctt ggtgcccaga agcagctcgc cgggctgcac gggtgtcgcg
     1861 atgaagctgc cgagatggtc ggacgtcatg agccgcaccg tggtggggct aagcacgcgg
     1921 gcgccctcga gaacgccacc gttcatgagc atctggctga agcgcaggta gtcggatgcg
     1981 gtggacacgc cgccggcacc gccggagtcg ttcttcggca cgctggagac gtcgatgagc
     2041 gggaagccgc gattggcgaa gcggtccttc tcgagggtct cggccagtcg cggcatcttc
     2101 gcgggcggaa cccagaaacc cgagtcgacc atcttgagcg gcttgaacag gcgtgcctcg
     2161 aggaaatccg cgaggcgcat ccccgaggcg gcttccacga cgcggccgag gatgtcgacg
     2221 ccgaggctgt agtgccacgt ggtgcccggc tggaacgcga gcggcaccgt ggccatgcgc
     2281 tccacctgct ccgcgggcgt cactccgcgc gcctcgtaat cgaggtcctt gcggtacacc
     2341 cccgccttct cgaggccttc cttcaccggc gcattggtcg tgatctcgcc gtatgcgagg
     2401 ccggcggtgt ggcgcagcag gtcctgcacg gtcatcggcc ggtccgccgc cgccagcgtg
     2461 taggccacgt tgccgctggc atccttgccc gcgacgctca cctgcagctt gtcgaacccg
     2521 gccaggtact tgcccaccgg gtcggtgagc tgcaccttgc cttcctcgac cagcatcatc
     2581 gccgcgacgg acacgagcgg cttggtcatc gagtagatgc ggaagatcga gccctcggac
     2641 atcttcgtgg tcggcgtctg catcccgaac gcctcgtgat acacgagctt gcccttcctc
     2701 gcgaccatca ccaccgcgcc gcggaagctt ccgtcggcga cttcctttcg catcgcctcg
     2761 ccgatgagcg cgagcttctg cgacgacatc cccacggctt ccggcttcgc gaccggaagc
     2821 ggggcaaggg cgagggcgac ggcagggacg aacgaggcaa gagcgatcca caaacgcgcg
     2881 gtgcgcctgg cgagcagggt tgtcatgtca tttcctcccc gaaaaagcgg tcactctcga
     2941 ccttgctttc gcgcccgtca agggcgggtg cggcacgtcg gccgggcgta gcgcgataat
     3001 cgccgcgctc acccggttcc ctcccaggag aatcccccat ggccccctcg cccggcgcgg
     3061 cgcaccaacc ctacatcgcg gcctcggacc acgtcgccga gttcaccgtg cgtgccgtgc
     3121 tcatgggaac ccttctcggc atcgtgttcg gcgcatcgtc gctctacctg gtgctcaagg
     3181 tcggcctcac ggtgagcgca tcgatcccgg tggcggtgat cgcgatcacg ctctttcgcc
     3241 tcgtcgccaa ggggggcggt cgggattcga cgatcctgga gaacaacatc gtccagaccg
     3301 cgggttccgc gggcgaatcg atcgccttcg gcctgggtgt gacgatgccc gcgatcctga
     3361 tcctcggctt cgacctggag atcgtacgcg tcatgctggt cggcgtactc ggcggggcgc
     3421 tcggcatcct gatgatgatt ccgcttcgcc tcacgctcat cgtcgcccgg cacggtgagc
     3481 tcaagtaccc cgagggcacc gcttgcgccg aggttctcaa ggccgcggaa gccgcgaagg
     3541 catcgcgcgg ttcgcgcggc acgggtgccg ccgacgacgc gagccgcgcc ggcgtcatct
     3601 tcctgggctt cgccatcggg ctggcctaca aggccgccaa cgtcgcgctg aagggctgga
     3661 aggatgtccc ggagaaggtc ttcgacgcgc cgctcaaggg cggctccgtg ggcgtggaga
     3721 tctctcccga gctgctcggc gtgggctaca tcatcggccc gcgcatcgcc tcgatcatgt
     3781 gcgcgggcgg ggtgctcgcc tacctgctgc tcatcccgat gatcagcttc ttcggcgacg
     3841 cgctgcaggt ccccgtggcg ccgggcgtga agctcatcca cgacatggac cccggcgaaa
     3901 tccgcggcgc ctacgtgctc tacatcggtg cgggcgccgt ggccgccggc ggcatcgtga
     3961 gcctcgtccg cgcgctgccg acgatctggc gcagcctgcg cgaaggactc aagggtttcg
     4021 gcgaggccgg ggacgcaagt acccggcgca cggaccggga catcccgatg aagtgggtgc
     4081 tcgtcggcgc gctcgccatc atcgtcgcca tcacgctcgc gccgccgctg cacatgaacc
     4141 tgctgggcgc gatgctgatc ctggtcttcg gattcctgtt ttccaccgtc tcctcgcagc
     4201 tcaccggcga gatcggctcg tcgagcaacc ccatttccgg gatgacggtc gccacgctcc
     4261 tgctcacctg cctcatcttc ctgctggtgg gctggacggg cggcccgtac tacgtgaccg
     4321 cactttccgt gggcgccatc gtgtgcatcg cctcgagcaa cgccgggacg acgtcgcagg
     4381 acctcaagac cgggttcctc gtgggcgcca cgccgcgcct gcagcagttc gcgatcctcg
     4441 ccggcgcgct cgcctccgcg cttgtcctgg ggccgatcct gctcaggctc aacgatgcgg
     4501 cgacggtcta cgtcccgatc gcccaggtgg cgccaggcct tgctaccgac gcgcaatccc
     4561 tcaccgagag ggccaccctg caaggcccgc aggcggcgca ggacggcggc acctatcgcg
     4621 tctggcacaa gacggacctg gccggcgggc ccgccggcaa gtacctcgtg gatgacgcgg
     4681 gccgcgccgt ctacctcgtc gatccgggca tcaacggcag ccacggcacg cggcccgacg
     4741 gcaccgaggt gcgcaagtac gacgcgccca aggcggtgct catgtcctac atcatcaagg
     4801 gcatcctcga ccgcaagctc ccgtgggcac tggtgatctt cggcgtggtc atcgcgctgg
     4861 ttctcgagat gtccggcatc gcctcgctcc ccttcgccgt cggcgtgtac ctgccgctat
     4921 cctcgtcggc tcccatcttc atcgggggcg cggtgcgctg gctcgtcgac cgccggcgca
     4981 accgccaggc gcagtaccgc gatgcgagcg aggcggtgcg ctctgccgcg ggcgaccgga
     5041 gtcccggcgt gctgctcgcc tcgggctaca tcgccggcgg ggcgctggca ggaatcgtca
     5101 tcgcgttcac ggcgggcgtg ctttccggct tcgacaaggc ggtgaccgac tgggccgccg
     5161 cgaacaatcc cttcttcgcc ggaccgtggg cggacctgct ctccctcgtc cccttcctgg
     5221 gactcgccgt cctgctccac gccgtcgggc acgaaaagcc gccgcgggaa tcgggagcgt
     5281 gatgccggcc ggtcgcatcc cgctttgcga atcgagagtg gcgacatgaa ccggcaatga
     5341 gtcatagtta ggggtggcgt cagggcaacc tgcctgccgc gcgacacccc ggctccatga
     5401 acatcgtcaa tctttcgatc cccgaagtcc tggccagcct caagacttcg gagggtggcc
     5461 tagacgcggg cgaggccgcc aggcggctgc gcgagttcgg ccacaaccgc atcgaggagg
     5521 tcaagggcaa gcccctgtgg ctgcgcttcc tgcgcgagtt cacgcatttc ttcgcgctga
     5581 tcctgtgggt cgctgccgcc ctggcgttct tcgccgagtc gagaagccgc ggcgagggca
     5641 tgtggcagct cggcgcggcc atcctcgccg taatcgtgat caacggcgtg ttttcgttct
     5701 ggcaggagta ccgcgccgaa cgcgccatct cggccctgcg gcaattgctg tcgccgcagg
     5761 ccaaggtcct gcgcggcggg ctgttgcagg agatggccgc cgagctgctc gtgccgggcg
     5821 atgtcgtcct gctcggcgag ggcgacaacg tgccggcgga ttgccggctc atcgaggcgt
     5881 cccgcctgcg cgtcaacctc tccaccctta ccggcgagcc cctcgccaag ccgaggacgg
     5941 cggaaggaat cgccgatggc cgggcgctcg atgcgaagaa cctggtcctc gccggcacct
     6001 cggtgatttc gggagagtgc cgggccgtgg tgttcgccac cggcatgcgc accgaattcg
     6061 gcaggatcgc ccacctgacc cagaccgcgg gcgaggccgg ttcgcccctg caacgcgaga
     6121 tcgcccgcct gtcgaagctg gtggccgtac tggcctcggt ccttggcctc acgttcttcg
     6181 gcatcggcac ggccatcggc ctgcccttct gggacaacgt catgttcgcc atcggcatca
     6241 tcgtggccaa cgttcccgag ggattgctgc cgaccgtgac gttgtcgctt gcgatggcga
     6301 cccagcgcat ggcccggcgc aacgcgctcg tgcgccacct gccctccgtc gagacgctgg
     6361 ggtcggccac cgtcatctgc tgcgacaaga ccggcacgct cacgcagaac cggatgtccg
     6421 tgaagcaggt gtacgcggcc cgcctgcagg gtccgacgaa cgacctgcgc agcattgccg
     6481 gcgacatcca cctccagcgc aatgcgcgtt actgccacag cctgaagcgc ggcagccgca
     6541 acgggaagca agcctggctg ggagacccga tggagatcgc gctggcggag ttcgccggcg
     6601 ccgacaatgc ccaggacgac ggcgcggtca cgggcgacat tcccttcgac agcgagcgcc
     6661 ggcggatgtc tgtcgtcgtc gagcgcgatg gtgaacggtg gctctactgc aagggggctc
     6721 ccgaggtggt gctgctcctg tgcgaccgca tcgacgatgg cgacaccacg gtgcccctcg
     6781 atgaagaggg ccggcgccgc ttcaccgagg cgcaggacgc catggcgcaa ggcgggcagc
     6841 gcgttctcgc tttcgcctgc cggaaactgg acgccgatga aggccccgag gagaggaacc
     6901 tcgcgctgtc gggcctcatc ggactgcacg acccgccgcg ccccgaagtg cacgaggccg
     6961 tatcccgatg ccgcacggcg ggcatcaagg tgatcatggt caccggcgac catccggaca
     7021 cggccgtcgc gatcgcccgc gagatcggtc tcgtgcggtc cgcgagcccc gcggtcgtgc
     7081 agggaggcgc gctgctgaag atgacgccgg cgcaactgca gatcgcactg gacagccccg
     7141 agatcctgtt cacccgcgtc acggccgagc agaagatgct ggtggtcgaa gggctcaagg
     7201 ccaaggggca cgtggtggcg gtcaccggcg acggcgtcaa cgacgcgccg gcgctcaaga
     7261 ctgcgcacat cggcatcgcc atgggaatct ccggcacgga cgtcgccaag gaagcggccg
     7321 acatgatcct gctcgacgac aacttcgcga gcatcgtcaa cgcgatcgag gaagggcgcg
     7381 cggtattcga gaacatccgc aggttcctca cctacatcct gacatcgaac attcccgaac
     7441 tggtccccta catcgccttc gtgattttcc ggattccgct gccgctcacc atcatccaga
     7501 tcctcgcggt cgacctgggt accgacatgc tgccggcgct ggccctggga gcggagcgac
     7561 ccaatcgcct gctcatgcag cgaccgccgc gcccggccgg ggaacgcctg ctcacctggc
     7621 cgctgctggc aagggcctac ctgtggctgg gggtgctgca ggcggccgtg tcgatggcgg
     7681 cgttcttctt cgtcctgcag cggggcggct gggattacgg cgatatgccc ggcaagtccg
     7741 atcccctgta cctgcaggcg acgaccgcgt gccttgctgc catcgtcgtg gcgcaggtgg
     7801 tcaacgtgtt cgtgtgccgg catccgcacg agtcgatcct tcgcttccgc ccggcggaca
     7861 acccgctgct cctggcggga atcgcgttcg aggtcctgct gatcctcgcg atcgtctaca
     7921 cgcccgtcgg gaacggggcc ttcggaaccc ttcccctgtc gtgggagccg tggctgctca
     7981 tgatcacgct cgcgctgggc atcggcctgc tcgaggaact gcgcaagctt cttgccggtg
     8041 ccggacgccg tgcctgaaac cgcgaccggc tccgacaacc gcgtttcagc ccagcatgtt
     8101 cgtgtcgccg caaaccgaga gggcctgccc ggagatcatc cggccgcgcg ggctggcgac
     8161 gaacgcgacc aggtccgcga gctgctgggg cgtcacgtac tccttgagcg aactatagga
     8221 gaaggcaatc gactccatct cctcgaagga gatgccgcga gcctcggcct tggccgcgag
     8281 gacgcgagcc tgccgctcgc cggcaatgac gcccggcagg agcgcattca cgcggatatt
     8341 gtccgggccc agctccaccg cgagcgagcg cgtcagcccc accacgcccc acttggcgga
     8401 agcgtaggga gtgcgcaggg cgaagcccag ccgccccgcg agcgaggaga cgttcatgag
     8461 gctggcgttc cccgagcggc gcaggtgcgg cacggcgagg cgcacgcagt tgaact
//
LOCUS       DASUEV010000083        21352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1513070, whole genome shotgun sequence.
ACCESSION   DASUEV010000083 DASUEV010000000
VERSION     DASUEV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 21352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21352
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513070"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFV55_02575"
     CDS             complement(<1..317)
                     /locus_tag="VFV55_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4943208.1"
                     /translation="MKLYYFPGACSIAPHIVAREAGIALELEKVDLGSKRTATGEDYT
                     TVNPKGYVPALRLDDGSVLTEVSALIQYLADRAPGSGLIPAAGTMERYRVLEWIGFIA
                     TEI"
     gene            447..1100
                     /locus_tag="VFV55_02580"
     CDS             447..1100
                     /locus_tag="VFV55_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX4943209.1"
                     /translation="MSERYLVYFADPMCSWCYGFGPELEALLRDRPGLRMNVVMGGLR
                     PYNTEKASPAFRETITGHWAHVARESGLTFSDAALAREGFVYDTEPACRAVVTVRATD
                     PARALPYLKRVHAAFYAQGRDVTQPDVLADLAADQGLDRAAFLSAHAAPAAREAVRQD
                     FAATQQTGVTGFPTLAVAYPGRGHYLVSAGFTRTAALVEQLDRIDEIAGEDSARQAS"
     gene            1206..1763
                     /locus_tag="VFV55_02585"
     CDS             1206..1763
                     /locus_tag="VFV55_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943210.1"
                     /translation="MGPERLPPIPDASLTPEQKRAATELATGPRGAVFGPFVPLLRSP
                     DFLSPLQKAGEYLRYKSALPPRLSEFAILIVARHWNQQFEWHIHHPIAERAGVPAGHI
                     RAVAENRRPDNMAADESVVHDFLQELQENRAVSDTTYGRAVELFGERGVVDLCGLCGY
                     YSLLAMVMNVARTPLPDGVPPPFES"
     gene            1774..3033
                     /locus_tag="VFV55_02590"
     CDS             1774..3033
                     /locus_tag="VFV55_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4943211.1"
                     /translation="MHGRRRFLRDCLAILSAAPALAKAASVPPRPAADFEALWHAIDA
                     GYAYLASGQDWRTAAGRWRPRAAAARSREELLAALEGVIDELNDEHLTLDAHLPGSPR
                     PVPMATDIWAEWVGKEAVISAVRAGSVADVAGAVPGMHVISTGGMPVDRAVRAMLRRN
                     RQADPRARDWALRRLLAGPWSGTLTFEVSSAGRASHLEIERQDLPPSGTPPLIARRIG
                     EDRDLGYLRVKNNLDDAGLVQHFDAALQLLKGTRGLILDLRETQSGGSPDVARALLGR
                     FVTAESPWIVRAPRGARRGADAPPERISPRGPFPYTAPTLVLVDRWTAGEGESLAIGL
                     EAAAKATLVGTAMAGLRGDIRTLRLPQSGIVLRYPAARVFHANGTPREVVRPAVAVDI
                     VAPSGGPGDPILYQALKLLGSGAAPGR"
     gene            3135..4094
                     /locus_tag="VFV55_02595"
     CDS             3135..4094
                     /locus_tag="VFV55_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943212.1"
                     /translation="MNSIVPLRHRTPTPPEDERGIVAAWLPEATGTDPAALHRLAAGP
                     SRRLGELVTDVNPACESVVAEALENASRTGRRVGEALVEKGVLTPGERDSLVEFQRHQ
                     RGEAPTEDRLRLGQILVTLGHISESNLAEALEGQRTSKRPLGEELVARGRISDDVLQS
                     ALGTQRRLVVAALVAALAMASPGTMAPAEAAQSGTHSINVAVRVLPFVRIKVLRQPES
                     MEITKEDAERGYVEIRSASHLHVTANTPWEVYFKPRGEVALSARVSGLEGDVVVGPAG
                     GRFAGLRPQSNSKDFDLSYRFDLAPGVGPGTYPWPLTISVQGA"
     gene            complement(4114..4536)
                     /locus_tag="VFV55_02600"
     CDS             complement(4114..4536)
                     /locus_tag="VFV55_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX4943213.1"
                     /translation="MALQLNLPTGLTGTAEMVVGEQHTAPRIGSGRIHVLATPVMINL
                     IEAAALAAVEQSLPEQHQSLGTRLDISHIAATPVGMRVRATAEVVGVEGRTIRFRVRA
                     EDEHDLIGEGTHERVVVNLERFDERVREKAAREGREKP"
     gene            4656..6485
                     /gene="mnmC"
                     /locus_tag="VFV55_02605"
     CDS             4656..6485
                     /gene="mnmC"
                     /locus_tag="VFV55_02605"
                     /inference="COORDINATES: protein motif:HMM:NF002483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEX4943214.1"
                     /translation="MTGRSVCVTPARLERTPDGVPYAPEFQDIYHSAAGGLAQARHVF
                     LAGSGLPERWRGRDSFTILETGFGLGINFLAAWDALRADPAGPARLHFVSVERHPFAA
                     GDLADALAPFEELRPLATALLAIWPPPLAGFHRLQFDGGRVQLTLMAGDALELLPQLE
                     ARADALFLDGFSPAKNPEIWSPEVVRELARLAVPGATLATWTVAGGVRAALAGAGFRV
                     EKRDGFGFKREMLAGVREGEVAAPARARRHAVIVGAGLAGIFCAERLASRGWETDLID
                     VRERRTGATVGLVRPIANIRDAVNAQASRPAFLYALQHFRALQHDGYHLVWNRCGVLQ
                     LADGDDEAARFAAIADSQCHPPEFLRFVDAAGARAIAGRPVRGPGWWMPSGAWVSPGS
                     LAIALLGRAGSRVRRHVGRAVERLELEGADWRAIDADGSVIAEAPTLVVANAFDAKRL
                     LPDARLRLALVRGQVTFLPPSAARTLDVIVSGSGYVAPLPDGGHVVGATYQHDDTGED
                     VRAADHRENLARAGKMLPGFADDVHPMTLSGLAGFRATVPDRLPIFGATAIPGIWTAT
                     GLGSRGLLWAPIGAELIASRLEGEPSPLPRDLAGAISPRRFLS"
     gene            6656..7666
                     /locus_tag="VFV55_02610"
     CDS             6656..7666
                     /locus_tag="VFV55_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4943215.1"
                     /translation="MQVTLKFVSSAAALWLSAGLSWAQGLIVDTAYVENAAKHGALVW
                     DARSEDDYSAGHVPGAVNIGNAGSVFRDMNREDPPPAAVAAKIFGNAGMDILNREVIV
                     YTRKGDSFAYFAARMVEYYGGRHAKVYHGGLDDWKAAGKAVEKDAPRLKPVSLTLTTE
                     GNGAIGNKEMVERVRAGGAQIIDTRTVKEYTGEDIRAVRGGHIPGAVNIPYEANWQDP
                     AAAGKLASKQVSNRDGMALKSPEDLRKLYAKLDPSKETIVYCQSGVRASETAVVLREL
                     GFKDVKVHEPSWLGYAGVLSAPAEDEVFVNVGALNGMVSSLAVRVRDLEAEIAKLQTA
                     RR"
     gene            complement(7820..8986)
                     /locus_tag="VFV55_02615"
     CDS             complement(7820..8986)
                     /locus_tag="VFV55_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4943216.1"
                     /translation="MNLSKKTLGYAGASLVVVGLLAWWAWPGPRIVETTRAARGPVQV
                     TIEEDGETRAVDRYGIAAPVAGRLLRVVLREGDAVKAGQTVAEIAPLPISEAERDMLE
                     AKLDAARALRLEADERVRSAQTDAAQAERERVRVADLVAKKFMSEQALEQADVTAKMK
                     VVEVKAARSRAAAAAADIEAARATLTAVRDKAPMVRVTSPVSGSILRIAEKSERVVQP
                     GTTLVTVGDPLALEIVVDVLSNDAVRVKPGMRMLITGWGGSETLEAVVRTVEPAAFRK
                     ISALGVEEQRVNVVADFKAVPPGIGDAYRVDARIVVAEKADALRVPVGALFRREGKWT
                     VFQLTDGRAKARSVDVGLRGAREAEIVAGLAENDEVVLYPGNDLIEGARVVARK"
     gene            complement(9000..11369)
                     /locus_tag="VFV55_02620"
     CDS             complement(9000..11369)
                     /locus_tag="VFV55_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4943217.1"
                     /translation="MRVGALDRKILRDAWHMRGQILACALVVAGGINSFVSLSSVNRT
                     LAASQAAFYEEYRFADVFAGLVRAPEHVSAALGAIPGIATLETRVAVRVNLDVPGLAE
                     PARGRIISISRDAAGGLNALHLRRGRMPAAMSQDEVLASEAFAIANGLQPGDSIRGVM
                     NGRYKKLTVVGVAISPEFIYALGPGQLVPDNRRYGVLWMDRRGLATAYDMEGAFNDVT
                     ARVSPGASVPAVLGEMDRLLATYGGIGAYDREDQLSHRFLSDEIAQNRTMSRITPTIF
                     LLVGAFLLYTILTRLVQMQRSQVGLLKAFGYSGAEIALHYLKLALVISLIGAALGVAT
                     GLPFARYMLAMYAEYYYFPMYGNDQVASVAAAGVALAVAAAALGAVPAALRGARLAPA
                     DSMRPEPPPRFHAGVLERWGLRRALPPAGRMMIRNLARRPLRFIANVTGIAIAVSLLV
                     MMSAMLDGVRRLMEWQFTIVQREDIQVLLVEPRTRDALDSFRAMHGVTEAEPFRLAAA
                     RLHSGYRMKRGLLFGLEEGPTLRRVVGGDGRAYAIPREGVLLTAIFAGSLGVRAGDSI
                     EVEVMEGKRARETVLVVGIVDEPIGYAAYVSRATANRLMREGDVLTGVYLRVDADERA
                     RIFAELKAMPLVAGANLREAAYAQFNAMIDRSVGIMITVNLVFAWIIAFGLVYNGARI
                     ALSERGYELATLRVLGFTQRETASLLLGEQGVITLVGLPAGLALGIALTLYFIWMLST
                     QMWRMPYVMTGANLVGAMVAVAAAAVASGGAVAWKLRRLDLVAALKARE"
     gene            complement(11366..12073)
                     /locus_tag="VFV55_02625"
     CDS             complement(11366..12073)
                     /locus_tag="VFV55_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4943218.1"
                     /translation="MSDPVFRMRGVSKVYHVGELEVVALREVSLELFAGEFVVLLGPS
                     GSGKSTLLNLLGGLDTPTAGEVDYLDHSLSESGDEQLTRFRREHVGFVFQFYNLIPSL
                     TAAENVALVTDIAAKPMDPRDALALVGLAERADHFPAQLSGGEQQRVAIARAVAKRPD
                     VLLCDEPTGALDVATGRLVLEVLERVNREMGTITAVITHNASIAAMGDRVIRMSSGKI
                     IEIAGNATRLPAAELVW"
     gene            complement(12158..13564)
                     /locus_tag="VFV55_02630"
     CDS             complement(12158..13564)
                     /locus_tag="VFV55_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4943219.1"
                     /translation="MPGSRTSRGAFAALCALVVLVYLPGLAGPFQFDDYFTVATDPGA
                     RSLVAWWENVTSHVRPLLKASFVLTGALGQAVGDVPTGHRIGNLAIHLGATFTFYLFG
                     LRASTALEPWRDMQSRNAHALMAAAVFALHPLSTEAVSYLSARSSSLATLASVLALLA
                     WLNAREARGAQRFAWILAGVASWAIASGSREVAAATPLLWLLLEWLRAPMEIDPRARR
                     RVLLGLACVIALAVVAFIAWMAIHPRYGPLLDMSARILAARMREPVMATALGYLACVA
                     ALACAPNIDPTPSPATGLQALVLAGCLVLVGVLAVRARRRHPMALVALAWAALWLLPI
                     YALPIRHDIVAERHAYPAVWSLGWLVGAIVVPVAAARERAVRIGGRVLAMLLVSVLAL
                     LSAARNREYRSEESLWEAAARDSASKLRVLNNLGAAYIEAGQWDEAQRALRAAQKLDP
                     DNEIVGINLDRALRRSPN"
     gene            complement(13611..14315)
                     /locus_tag="VFV55_02635"
     CDS             complement(13611..14315)
                     /locus_tag="VFV55_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943220.1"
                     /translation="MMSRLKILNGAPVALLLAFAVAGPARAQGSACACADLADLRNRY
                     CEARAAIGEYARQIAKIRAQETKTGAPVMYTVARYAEHVQPCVQEAINQVTDANANRP
                     TADTNNACIITYKGNPTACLKQVLGAHESVHVTVCQKWTNDRDKEGFFASLRGLFTDF
                     REGTTLVDLLNEERAAYQTEMNHVRDDLARLSRSKPPCPGMPAEPPGPPRIPTIEPCP
                     PPKPRPDAQDSSCKHR"
     gene            complement(14341..15948)
                     /locus_tag="VFV55_02640"
     CDS             complement(14341..15948)
                     /locus_tag="VFV55_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943221.1"
                     /translation="MEERNATEPKRGNFLSEGFRELGRKIDRAKLRSLMRKQETDRAA
                     ALVALGQRAWEEKIDLSAFAELRDRLTGLDARAGELSATASKLDAEKSTLETERRAEL
                     EKFAARRKAVEEKKSPIDAALRTSRSAKTAIEQAIKQSESRLAAIAGKLSTLDRDIAA
                     FGTAAAPGQGPKLAAAQAERAKLAAEQVDLGARMAKAREDLPAHVTEESRLSGESQKY
                     AAEIAVIDAEQKASIAHIDENLARVRKESQGASQQASAVQKDRSGNLGSLGQALYEAK
                     VAAPQLAEPAGRVAAIDRARAEAQSTLDASTAESQLLSGATMATFWSVLVGVPLLLAA
                     LGVGTWQFLHRRAAPAPVAIQATTVPGKGCTYQQPPDSGEGVSISSDCTRTEGTFVEG
                     RLHGKGRKAWPNGELMEGQFYSGSLYGPGLHVTRDGRRIEAAFSGGRPIGPGKLTMPD
                     GTVFEGRFWGPAIVGWGVRRSPNGEVLAGDWREAPGGGMKPFGLMLRVRPDGTREKVE
                     AAALDPASAPSAAPATQAQPVSDDKLY"
     gene            16439..17023
                     /locus_tag="VFV55_02645"
     CDS             16439..17023
                     /locus_tag="VFV55_02645"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEX4943222.1"
                     /translation="MRQFFNFIAGAALLLALSGCAPLLTQSPGRGLSPVNAVSDGGDE
                     HLILFGHDVVSYFTEGTHQRGLPAIKSVYKGVTFRFASPEHKALFDQSPERYLPQFNG
                     YCANGMVYGIPWGGDADTWKMIDGKLYIFGGAGSRDAFLLDVPRNMALAHRYWNQEVN
                     GSNAFVQRAWRLVVRVPHYKSGAQLAEEVAKAKR"
     gene            17028..>21352
                     /gene="uvrA"
                     /locus_tag="VFV55_02650"
     CDS             17028..>21352
                     /gene="uvrA"
                     /locus_tag="VFV55_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX4943223.1"
                     /translation="MNELSQVPGIDPGPLDAESGGAISIRGARQNNLKNLDVDIPTGE
                     LVVVTGPSGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDSIEGIPPAI
                     AIDQTNPVRTSRSTVGTMTELNDHLKLLFARAGHLFCRNCSRPVRRDSPESIFAEIAE
                     RAKGADDPRVAITFPIEVPKNFTEEEVTRHLEAQGYARIHRRKGARLDVVQDRLRLGN
                     AEKARVVEAIEAALRVGAGRVNVFVEDSADAWRFSTDLHCPDCDIHYSDPSPARFSFN
                     SPVGACDKCRGFGRVIGVDYGLVVPDEAKSLGNGAVKPFQTASFDECQDDLEKYAKRR
                     GVALDIAWRDLPAEHRRWVIEGDEDWIGWKKTGKTHWYGVQRFFDWLETKAYKMHIRV
                     LLSRYRAYTPCADCDGARLKPESMLWRVGTKTEADAVLAPERRYPPRNTKWNLDELAA
                     LPGLCLHDVMLLPIERARTFFERLALPAPMDEAAELLLEEVNARLRFVCEVGLGYLTL
                     DRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMGRVIGVMKRLRDAG
                     NSLVVVEHDPQIMLEADRLLDMGPGPGERGGEIVFFGTPDGLRQAKTVTAEYLSGRRR
                     ADAGVVSQPPAADTPRLAIRGVTEHNLKGIDVEIPLHRFVCVTGVSGSGKSTLIQDVL
                     HPALLKHLGKPTESPGAFREMHGAGRISDVVFVDQSPIGRTTRSNPASYVGAFDAIRA
                     LFAKTQDAKGRGYTAGTFSFNSGNGRCPTCSGNGFEHVEMQFLSDVYLRCPDCNGRRY
                     RDEILEVKWGGRNIAEALELTVHEALDFFAGEPDVVARLRPLADVGLDYLRLGQPVPT
                     LSGGEAQRLKLAGHLIEASGRPVAMLPRRPADGIAPALHPDVAPARTRLFLFDEPTTG
                     LHFDDVAKLLAAFRRLIAAGHSLVVIEHNLDVIRACDWLIDLGPEGGDAGGDIVFAGT
                     PSDATLHPASHTGKALLDYDKALHSTDHLRSSVFICGSQDLRSNYSPTHPFSKNIQVR
                     HAREHNLKNIDVDIPRGKFTVITGVSGSGKSTLAFDILFAEGQRRYLESLNAYARQFV
                     QPASKPDVDAIFGIPPTVAIEQRTSRGGSKSTVATQTEIHHFLRLLFVKLGTQHCPDC
                     DVPIEPQSEEAIAARLFREHRGERIALLAPLVVSRKGYYTDLAKWAGSKGVSHLRVDG
                     EYLSTGRWPRLDRFREHTIEMPVAEVEVTPANEVALREAIAAAVEHGKGVVHALPGGW
                     GATHSVNFSTKRACRSCGRSFPELDPRLFSYNSKHGWCPACYGTGVKLEGSGIDWNDE
                     RAKTGTEDHVLDSWVSWLELDEACPACAGRRLNREALAVRFRGQSIAAMSAQPIEAAQ
                     RFFRGLALTGRDQEIARDILAELASRLSFLEQVGLGYLSLDRGAPTLSGGEAQRIRLA
                     AQLGS"
BASE COUNT         3245 a         7601 c         7198 g         3308 t
ORIGIN      
        1 tgaatttcag tcgcgatgaa gccgatccat tcgagcacgc ggtaacgctc catcgtgccc
       61 gccgcgggaa tgaggccgga gccgggcgcg cggtcggcga ggtactggat gagcgccgat
      121 acttcggtca gcacggaacc gtcgtcgaga cgcaatgcgg ggacgtatcc cttggggttc
      181 accgtcgtgt agtcctcccc ggtggcggtc cgcttgctgc cgaggtcgac cttctcgagt
      241 tcgagggcaa tgccggcctc gcgggcgacg atgtgcggcg cgatggagca tgcgcccgga
      301 aaatagtaga gcttcatgga cactccgctt gggtagggtg ggaaagggac gttccgcgaa
      361 cgcaagatta ccgcggccag cggttcttcg ccggaagcgt ggcgcgcggc gcgatcgcca
      421 cgaatccggg cgcataatcc ggcgtcatga gcgaacggta tctcgtctat ttcgccgacc
      481 cgatgtgctc gtggtgctac gggttcgggc cggaactgga agcgctgctg cgcgacaggc
      541 ccggcctgcg catgaacgtc gtgatgggcg gcctgcggcc gtacaacacc gagaaggcga
      601 gccccgcctt tcgcgagacg atcaccgggc actgggcgca cgtggccagg gaatcgggcc
      661 tgacgttcag cgatgccgca ctggctcgcg agggcttcgt ctacgacacg gaacctgcct
      721 gccgggcagt tgtcaccgtg cgcgcaacgg accccgctcg cgcgctgccc tacctgaagc
      781 gcgtgcacgc cgccttctat gcgcagggcc gcgacgtgac gcaaccggat gttctcgcgg
      841 acctcgcggc cgaccagggc ctcgatcgcg ccgcgttcct gtccgcgcac gccgcgccgg
      901 ctgccaggga ggccgtccgc caggatttcg cggccacgca gcagacgggc gtgacgggct
      961 ttccgacgct ggccgtggcc taccccggcc ggggccacta cctcgtctcg gcggggttta
     1021 cgcgaaccgc cgcgctcgtc gagcaactcg atcgcatcga cgagatcgcc ggagaagact
     1081 ctgcccgaca ggcgtcctga aggcgtccct ttgacttgcg tcatcggcgc ggccgccgcc
     1141 gttccgttag catgggttga tcccggttcc cggccggaca cagagtcact cctcgaggag
     1201 cagccatggg gcccgaacgc ctccccccga ttccggacgc cagcctcacg cccgaacaga
     1261 agcgcgccgc caccgagctt gcgaccggcc cgcggggcgc ggtcttcggc cccttcgtgc
     1321 ccctgctgcg cagccccgat ttcctgtccc ccttgcagaa ggccggcgaa taccttcgct
     1381 acaagagcgc gctcccgccg aggctctcgg aattcgcgat cctcatcgtg gcgcgccact
     1441 ggaaccagca attcgagtgg cacatccacc atccgatcgc ggaacgcgcc ggtgtcccgg
     1501 cgggccacat ccgcgccgtg gcggagaacc ggcgccccga caacatggcg gccgacgagt
     1561 ccgtcgtgca cgacttcctg caggagctcc aggagaaccg cgccgtgagc gacacgacct
     1621 atggccgcgc cgtcgaactc ttcggggagc gcggcgtcgt cgacctgtgc gggctgtgcg
     1681 gctactactc cctgctcgcg atggtgatga acgtcgcgcg cacccccctg cccgacgggg
     1741 ttcccccgcc cttcgagtcg taggcatcga cgcatgcacg gacgccggcg attcctgcgc
     1801 gactgcctcg ccatcctctc cgccgcgccc gccctggcga aagcggcatc cgtcccgcct
     1861 cgccccgctg ccgatttcga ggcgctctgg cacgcgatcg acgcgggata cgcctacctc
     1921 gcgagcggcc aggattggcg aaccgccgcc ggccgctggc ggccgcgcgc cgcggcagcc
     1981 cgctcgcgcg aggaactcct ggccgcgctc gagggggtga tcgacgagct gaacgacgag
     2041 cacttgaccc tcgacgcgca cctgcccggc tcgccgcggc cggtgcccat ggctaccgac
     2101 atctgggccg aatgggtcgg caaggaggcg gtgatctccg cggtgcgcgc cggcagtgtt
     2161 gccgacgtgg cgggcgccgt gccgggcatg cacgtgatct cgaccggggg aatgccggtc
     2221 gaccgcgcgg tgcgcgcgat gctccgccgc aaccggcagg ccgatccgcg ggcgcgggac
     2281 tgggccttgc gacgccttct cgcggggccg tggtcgggca cgctcacctt tgaagtgagt
     2341 tccgccggcc gtgcgagcca cctcgagatc gaacgccagg acctgccgcc gtccggaacg
     2401 ccgccgctca tcgcgcggcg aatcggcgag gatcgggacc tgggctacct gcgcgtgaag
     2461 aacaacctcg acgatgcggg cctcgtgcag catttcgacg ccgcgctgca actcctgaag
     2521 ggaacgcgcg gcctgatcct ggacctgcgc gagacccagt ccggcggttc tcccgacgtg
     2581 gcgcgcgcgc ttctggggcg cttcgtgacg gcggaatcgc cgtggatcgt ccgcgcgccg
     2641 cgcggcgcgc ggcgtggcgc ggacgcgccc cccgagcgga tttcaccgcg cggcccgttc
     2701 ccctatacgg cgcctacgct ggtgctcgtc gatcgctgga ccgccggcga gggtgaatct
     2761 ctcgccatcg ggctcgaagc cgccgcgaag gcgacgctcg tgggaacggc catggccggc
     2821 ctgcgcggcg acatccggac gctgcgcctg ccgcaatcgg gaatcgtcct ccgctatccc
     2881 gcggcccgtg tcttccacgc caacggaacc ccgcgcgaag tcgtgcgccc ggcggtcgcg
     2941 gtggatatcg tcgctcccag cggcggcccg ggggatccca tcctctacca ggcgctcaag
     3001 ctgctcggat cgggcgcggc accgggccgc tagcgcccgg atcgccggtt ccatcggtaa
     3061 ccggggcggc cggcaggacc tggccagggg cgtacaatga attgcgtccg ttaaaccccc
     3121 tcgcgaagcc cgaaatgaat tccatcgttc ctttgcgcca tcggacaccg actcccccgg
     3181 aggacgaacg gggcatcgtg gcggcctggt tgcccgaggc caccggcacc gacccggccg
     3241 ccctgcaccg gctggccgcc ggcccctcgc gccggctggg cgaactcgtt acagacgtaa
     3301 acccggcctg cgaatccgtc gttgccgagg cgctcgaaaa cgcgtcgagg accggccgaa
     3361 gggtcggcga agcactcgtc gagaagggcg tgctcacgcc cggcgaacgc gattcgctgg
     3421 tcgaattcca gcgccaccag cgcggggagg cgccgaccga agaccgcctt cgcctgggcc
     3481 agatcctcgt cacgctcggt cacatctcgg aatcgaacct cgccgaggcg ctcgagggcc
     3541 aacggacatc caagcgcccg ctgggcgagg aactcgtcgc ccggggccgg atttccgacg
     3601 atgtcctgca gtccgcactc ggcacgcaac gccgccttgt cgtcgccgcc ctcgtcgcgg
     3661 ccctcgccat ggcgagtccg ggcaccatgg cgcccgcgga agccgcgcaa tcgggcactc
     3721 attcgatcaa cgtcgcggtc cgggttctgc cgttcgtgcg catcaaggtg ctgcgccagc
     3781 cggagtcgat ggagatcacc aaggaagatg cggaacgcgg ctatgtcgag atccgctcgg
     3841 cctcgcacct gcacgtgacc gccaacacgc cgtgggaggt ctacttcaag ccgcgcggag
     3901 aagtcgccct ttccgcgcgc gtgagcggtc tcgagggcga cgtcgtcgtc gggcccgccg
     3961 gcggcaggtt cgccggcctg cgcccgcagt cgaattcgaa ggatttcgac ctgagctacc
     4021 gattcgacct ggcgccgggc gtgggtcccg gcacctatcc ctggcccctc acgatttccg
     4081 tgcagggcgc ctgagcgcag ccaggcagtc ccctcagggc ttctcccggc cctcccgcgc
     4141 cgccttctcg cgaacgcgct cgtcgaagcg ctccaggttc accaccacgc gctcgtgcgt
     4201 gccttcgccg atcaggtcgt gctcgtcctc ggcgcgaacg cggaaacgaa tggttcgtcc
     4261 ctcgacgccc accacttccg ccgtggcgcg cacgcgcatg cccacgggtg tcgcggcgat
     4321 gtgcgaaatg tcgagccgcg tgccgaggct ctggtgctgc tcggggagcg actgctcgac
     4381 ggcggcgagc gcggcggcct cgatgaggtt gatcatgacc ggcgtggcga ggacgtggat
     4441 gcgcccgctg ccgatgcgag gcgcggtgtg ctgctcgccg accaccatct cggccgtccc
     4501 ggtgagtccg gtcggaagat tcaattgaag ggccatgggc tgggcgtttc gcgatccccg
     4561 aaaatcgggg ctagccgcca ttatctggga cttgccctct cctgtcgcca tcggcgtcga
     4621 gcagcccgcg cgggccatgg cggtatcatc acgccatgac cggccgatcc gtctgcgtca
     4681 cccccgcgcg gctcgagcgc acgccggatg gcgttccgta cgcccccgaa ttccaggaca
     4741 tctaccattc ggccgccggg ggcctggcgc aggcgcgcca cgtattcctc gccggaagcg
     4801 ggctgccgga gcgctggcgc ggacgcgact cgttcaccat cctggagacg ggcttcgggc
     4861 tcggcatcaa cttccttgcc gcgtgggacg cccttcgcgc ggatcccgcg gggcctgccc
     4921 ggctgcattt cgtgtcggtc gagcggcatc cattcgccgc cggcgatctc gcggacgccc
     4981 tggcgccgtt cgaggaattg cggccgctcg ccacggccct gctcgcgata tggccgcctc
     5041 cgctcgcggg tttccaccgc ctgcagttcg acggcgggcg cgtgcagctc acgctcatgg
     5101 ccggggacgc gctcgagttg cttccgcaac tcgaggcacg cgcggacgcg ctgttcctcg
     5161 acgggttttc ccccgcgaag aatcccgaga tctggtcgcc cgaagtggtc cgcgagctcg
     5221 cgcggctggc cgttccgggc gcgacgctcg ccacgtggac ggtggccggc ggtgttcgcg
     5281 cggcgctcgc cggggccgga ttccgggtcg agaagcgcga cggcttcgga ttcaagcgcg
     5341 agatgctcgc cggcgtgcgc gagggcgagg ttgcggcgcc ggcccgggcc cggcgccacg
     5401 cggtcatcgt cggcgccggg ctcgcgggaa tcttctgcgc ggagcgactc gcctcgcgag
     5461 gctgggagac cgacctcatc gacgtgcgcg aaaggcgcac cggcgcaacc gtcggcctcg
     5521 tgcgcccgat cgccaatatc cgtgacgcgg tcaacgcgca ggcctcccgg ccggcgttcc
     5581 tgtatgcgct gcagcacttc cgcgccctgc agcacgacgg ctatcacctg gtctggaatc
     5641 gctgcggcgt gctgcagctc gcggatggcg acgacgaggc ggcgcgcttc gcggcgatcg
     5701 ccgattcgca gtgccatccg ccggaattcc tgcgcttcgt cgacgcggcc ggggcgcgcg
     5761 cgatcgcggg ccggccggtg cgcggcccgg gatggtggat gccctcggga gcctgggttt
     5821 cgcctggcag cctcgccatc gcactcctcg ggcgggcggg ctcacgcgtg cgccgccatg
     5881 tcgggcgcgc ggtcgagcgc cttgaactcg agggtgccga ctggcgggcg atcgacgcgg
     5941 acggttcggt catcgccgag gcgcccacgc ttgtcgtcgc caatgccttc gacgcgaagc
     6001 ggctgcttcc cgacgcccgc ctgcggctcg cgctcgtgcg cggccaggtg acgttcctgc
     6061 cgccgtccgc ggcgcgaacc ctcgacgtga tcgtgagcgg cagcggctac gtcgcgccgc
     6121 tgcccgatgg cgggcatgtc gtgggcgcca cgtaccagca cgacgatacc ggcgaggacg
     6181 tccgggctgc cgaccatcgc gagaatctcg cccgcgccgg caagatgctt cccggcttcg
     6241 ccgacgacgt ccacccgatg acgctctccg gcctggccgg attccgcgcg acggtgcccg
     6301 accgcctgcc gatcttcggt gcgaccgcca tcccgggcat ctggaccgcc accgggctcg
     6361 gctcgcgcgg cctgctctgg gcgccgatcg gcgcggagct catcgcctcg cgactcgagg
     6421 gcgagccctc gcccctgccg cgcgaccttg cgggcgcgat ttcgccgcgc aggttcctct
     6481 cgtaggcccg ccgcttttca acgaagcgca agttatcgta tcctccgcga ccgggcgggc
     6541 gggtgcccga agtttgcggg cttcctgcca ttgctcgccg gatccacgcc ggaaggaatc
     6601 gtcagggaca acgagcggcg tcgccattcg ccgacgctac aaggagattt gaaccatgca
     6661 ggtaacgctt aaattcgtgt cgtcggccgc agccctctgg ctctcggccg gcttgtcctg
     6721 ggcccagggg ctcatcgtcg acaccgccta tgtggagaat gccgcgaagc acggtgcgct
     6781 cgtctgggat gcccgcagcg aggacgacta ctcggccggc catgtccccg gcgcggtgaa
     6841 catcggcaat gccggaagcg tctttcgcga catgaaccgg gaggaccccc cgccggccgc
     6901 cgtggccgcg aagatcttcg gcaacgcggg catggatatc ctcaaccggg aggtgatcgt
     6961 ctacacgcgc aagggcgact cgttcgccta tttcgccgcg cgcatggtcg agtactacgg
     7021 cggcaggcat gccaaggtct accacggcgg actcgacgac tggaaggcgg ccggcaaggc
     7081 ggtcgaaaag gacgcgccca ggctgaagcc cgtttccctc accctgacga cggaaggcaa
     7141 cggggcgatc ggaaacaagg agatggtcga gcgcgtccgc gcagggggcg cacagatcat
     7201 cgacacccgc accgtcaagg aatacacggg cgaggacatc cgcgcggtcc gcggcggcca
     7261 tatccccggc gccgtgaaca tcccgtacga agccaactgg caggatcccg cggcggccgg
     7321 caagctcgcc tcgaagcagg tttccaatcg cgacggcatg gcgctcaagt ccccggagga
     7381 cctcaggaag ctctacgcga agctcgatcc gtccaaggag accatcgtct attgccagag
     7441 cggcgttcgt gcttcggaga cggccgtcgt gcttcgcgaa ctgggcttca aggacgtgaa
     7501 ggtccacgag ccttcatggc tcggctacgc cggcgtcctt tccgcaccgg cggaggacga
     7561 ggtcttcgtg aacgtgggcg cgctcaatgg gatggtctcc tcgctggccg tgcgcgtgcg
     7621 ggacctcgag gcggaaatcg ccaagctcca gactgcgagg cgctgatccg cgaaggaaaa
     7681 gggggtcaag gaaaaggggt caggttactt ttcagcccgc cgtctagcga cggcaatacg
     7741 acagaagagt aacctgaccc cttttccgtt ttgcctcttt tttgtgcaaa gtaacctgac
     7801 cccttttcct ccttttccgc tacttccgcg cgaccactcg cgcgccctcg atgaggtcgt
     7861 tgcccggata gagaacgact tcgtcgttct cggccaggcc ggccacgatc tcggcttcgc
     7921 gtgcaccgcg caggcccacg tccacgctgc gcgccttggc gcggccgtcg gtcagctgga
     7981 agaccgtcca tttcccctcg cggcggaaca gcgcccccac gggcacacga agcgcgtcgg
     8041 ccttctcggc gacgacgatg cgcgcgtcga cgcggtaggc gtcgccgatt ccagggggca
     8101 cggccttgaa gtccgccacc acgttcacgc gctgttcctc gacgccgagc gcagagatct
     8161 tcctgaaggc cgccggctcg acggtgcgca cgacggcttc gagcgtctcg ctcccgcccc
     8221 agccggtgat aagcatgcgc atccccggct tcacgcgcac cgcatcgttc gacaggacgt
     8281 cgaccacgat ttccagcgcg agcggatcgc ccacggtgac cagcgtcgtg cccggctgca
     8341 ccacgcgctc gctcttttcc gcgatgcgca ggatgctgcc gctcaccggc gaggtgacgc
     8401 gcaccatcgg tgccttgtcg cggacggccg tgagcgtggc gcgcgccgcc tcgatgtcgg
     8461 ctgccgccgc cgccgcgcgc gaccgggcgg ccttcacctc cacgaccttc atcttcgcgg
     8521 taacgtccgc ctgctcgagc gcctgttccg acatgaactt cttcgccacg aggtcggcca
     8581 cgcgaacgcg ctcgcgctcg gcctgtgccg catcggtctg cgcgctccgt acgcgctcat
     8641 cggcttcgag cctgagagcc cgcgccgcgt cgagcttcgc ctcgagcatg tcgcgctcgg
     8701 cctccgagat gggcagcggc gcgatctccg ccaccgtctg ccccgccttc acggcatcgc
     8761 cttcgcgcag gacgactcgc agcaggcgcc cggcgaccgg tgcggcgatg ccatagcgat
     8821 cgaccgcgcg cgtctcgccg tcctcctcga tggtgacctg caccgggccg cgcgcagcgc
     8881 gggtggtctc gacgatgcgc gggccgggcc aggcccacca cgcgagcagg ccgaccacga
     8941 ccagggaggc accggcgtat ccgagtgtct tcttcgagag gttcattttc cgctccgcat
     9001 cattcgcgcg ccttcagcgc cgcgacgagg tccaggcgcc gcagcttcca tgccaccgcc
     9061 ccgccggacg ccaccgcggc ggcggccacc gcaaccatcg ccccgaccag gttcgcgcca
     9121 gtcatcacgt agggcatccg ccacatctgc gtggacagca tccagatgaa atagagggtg
     9181 agcgcgatgc ccagcgcaag ccccgcggga agcccgacga gcgtgatcac gccctgttcg
     9241 cccagcagga gggaggccgt ctcgcgctgc gtgaatccga gcaccctgag cgtggccagc
     9301 tcgtagccgc gttccgacag cgcgatgcgc gcgccgttgt acacgagccc gaaggcgatg
     9361 atccacgcga agacgagatt taccgtgatc atgatgccga cgctgcggtc gatcatggcg
     9421 ttgaactgcg cgtaggccgc ctcgcgcagg ttcgcgcccg ctaccagcgg catggccttg
     9481 agttccgcga agatgcgcgc ccgctcgtcg gcgtccacgc gcaggtacac gccggtcagc
     9541 acgtcgccct cgcgcatcag gcgattggcc gtggcacgcg agacataggc cgcgtagccg
     9601 atcggctcgt ccacgatacc caccaccagc accgtctcgc gcgcccgctt gccctccatc
     9661 acctcgacct cgatggagtc gccggcgcgc accccgaggc ttcccgcgaa gatggcggtc
     9721 agcaggacgc cctcgcgcgg gatcgcgtag gccctcccgt cgccgcccac cacccggcgc
     9781 agcgtcggcc cctcctcgag gccgaagagg agcccgcgct tcatgcggta gccactatgc
     9841 aggcgcgccg cggcaagccg gaacggctcg gcttccgtca cgccgtgcat ggcgcggaac
     9901 gaatccaggg cgtcgcgcgt gcgcggctcg acgagcagga cctggatgtc ctcgcgctgc
     9961 acgatcgtga actgccattc catcaggcgg cgcacgccat cgagcatcgc ggacatcatc
    10021 accaggagcg agacggcgat cgcgatgccg gtcacgttcg cgatgaagcg caggggacga
    10081 cgtgccaggt tgcggatcat catccggccc gccggcggca gtgcccgccg cagcccccag
    10141 cgctcgagga ctccggcgtg gaagcgcggc ggcggttcgg ggcgcatcga gtcggccggt
    10201 gccagccgcg cgccgcgcag ggcggcggga accgcgccga gggccgccgc agcgacggcg
    10261 agcgccacgc ccgcggcggc gaccgacgcg acctggtcgt tgccgtacat cgggaagtag
    10321 tagtactccg catacatcgc gagcatgtag cgcgcgaagg gcagccccgt cgccacgccg
    10381 agcgcggcgc cgatgagcga gatcaccagc gcgagcttga ggtagtgcag cgcgatctcc
    10441 gcccccgagt agccgaacgc cttcagcagc cccacctgcg agcgctgcat ctgcaccagc
    10501 cgcgtgagta tcgtatagag gaggaatgcg ccgaccagca ggaatatcgt cggcgtgatg
    10561 cgcgacatcg tgcggttctg ggcgatttcg tccgacagga agcggtgcga gagctggtcc
    10621 tcgcggtcgt aggcgccgat gccgccgtag gtcgcgagca ggcggtccat ttcgccgagc
    10681 acggccggca ccgacgcgcc cgggctgact cgcgcggtga cgtcattgaa cgccccctcc
    10741 atgtcgtagg ccgtcgcgag ccccctgcgg tccatccaga ggacgccgta gcgccggttg
    10801 tccgggacga gctggcccgg acccagcgcg tagatgaact ccggcgagat cgccacgccg
    10861 accaccgtga gcttcttgta gcgcccgttc atcacgccgc ggatcgaatc ccccggctgc
    10921 aggccgttgg cgatcgcgaa agcctcgctc gccagcacct cgtcctggct cattgccgcc
    10981 ggcatgcgcc cgcggcgcaa gtgcagcgcg ttgagccccc cggccgcgtc gcgcgagatg
    11041 gagatgatgc gcccgcgcgc cggctccgcg agcccgggaa cgtcgaggtt cacgcggacc
    11101 gctacgcgcg tctcgagcgt cgcgatgccc ggaatcgcgc ccagcgccgc ggagacgtgc
    11161 tccggggcgc gcaccagccc ggcgaacacg tcggcgaagc ggtactcctc gtagaacgcg
    11221 gcctgcgatg cggcgagcgt gcgatttacc gaggagagcg acacgaagga gttgatgccc
    11281 cccgccacca ccagcgcgca ggcgaggatc tggccgcgca tgtgccaggc gtcgcgcagg
    11341 atcttgcgat cgagggcgcc gaccctcacc acacgagctc cgcggcgggc aggcgcgtcg
    11401 cattgcccgc gatctcgatg atcttcccgc tggacatgcg gattacgcga tcgcccatgg
    11461 ccgcgatcga cgcgttgtgc gtgatgacgg cggtgatggt gcccatctcg cggttcacgc
    11521 gctcgagcac ttccagcacg agacgccccg tggccacgtc cagcgcaccc gtgggctcgt
    11581 cgcagagcag cacgtcgggg cgcttggcga cggcgcgcgc gatggccacg cgctgctgct
    11641 cgccgccgga aagctgggcg gggaaatgat ccgcccgctc ggccaggccc acgagggcga
    11701 gggcgtcgcg gggatccatg ggtttcgccg cgatatcggt gacgagcgcc acgttctccg
    11761 cggcggtcag actggggatg aggttgtaga actggaagac gaagcccacg tgctcgcggc
    11821 ggaaacgcgt gagctgctcg tcgccgcttt cggaaaggga atggtcgagg tagtccacct
    11881 ccccggcggt gggcgtgtcc agcccgccca ggaggttcag tagtgtcgat ttgccgctgc
    11941 ccgaggggcc gagcagtacc acgaactctc ccgcgaagag ttccagcgac acttcgcgca
    12001 gcgcgaccac ttccagctcc cccacgtggt agaccttgga gacgccgcgc atgcggaaaa
    12061 cgggatcgga cacgcacata cctcgcccgg ggacagtggt tccagcatgc gccggggtgc
    12121 ctgccgcgga ctgacttcga tcaagcccgg cgacgattca attcggcgaa cgccgcagcg
    12181 cgcgatcgag gttgatcccg acgatttcgt tgtcgggatc cagcttctgc gccgcacgca
    12241 gtgcgcgctg cgcctcgtcc cattgcccgg cctcgatgta ggccgccccg agattgttga
    12301 gcacgcgcag cttggaagcc gagtcgcgcg cggccgcttc ccagaggctt tcctcgctgc
    12361 ggtattcccg gttccgggcg gcgctcagca gcgcgagtac gctcaccagc agcatcgcaa
    12421 gcacgcgacc gccgatgcgc accgcccgct cgcgggccgc ggccaccgga accacgatcg
    12481 cgccgacgag ccatcccagg ctccagaccg ccgggtatgc atggcgttcc gcgacgatgt
    12541 cgtgccggat cggcaacgcg tagatcggca gcagccacag ggccgcccag gcgagtgcca
    12601 cgagcgccat cggatggcgc cggcgggcgc gcaccgcaag gacgccgacg agcacgaggc
    12661 agcctgcgag gacgagcgcc tgcaggccag tggccggcga cggcgtgggg tcgatgttcg
    12721 gcgcgcaggc cagtgccgca acgcaggcca ggtagccgag cgcggtcgcc atgacaggct
    12781 cgcgcatacg cgccgccagg atgcgcgcgg acatgtcgag caagggtccg tagcgcgggt
    12841 ggatcgccat ccaggcaatg aacgccacga cagcgagagc gatcacgcac gcgagcccga
    12901 gcaatacacg ccggcgcgcg cgcggatcga tctccatggg cgcccgcagc cactcgagca
    12961 acagccacag gagcggcgtg gcggccgcca cttcgcggct gccgctcgca atcgcccatg
    13021 acgcgacacc ggcaaggatc cacgcgaagc gctgcgcgcc gcgagcctcg cgggcattca
    13081 gccaggcaag caacgccagt acggaagcca gcgtggcgag cgaggacgaa cgtgccgaaa
    13141 ggtagctcac cgcctcggtc gacagcgggt gcagcgcaaa gaccgcggcg gccatcaagg
    13201 cgtgcgcgtt tcgcgactgc atgtcgcgcc acggttcaag cgctgtgctc gcacgcaatc
    13261 cgaacagata gaacgtgaac gtcgcgccga ggtgaatggc caggttgccg atgcgatggc
    13321 cggtcggcac gtcgccgacg gcctggccca gggcgcccgt gagaacgaag ctcgccttga
    13381 gcaaggggcg aacgtgcgag gtgacgtttt cccaccaggc aacgagcgag cgcgccccgg
    13441 gatcggtcgc cacggtgaag tagtcatcga actggaacgg tcccgcaagc ccggggagat
    13501 agacgaggac gacgagcgcg cagagggcgg cgaatgcgcc gcggctcgtt cgcgatccgg
    13561 gcatcatgtc agcgtgccga tcgtggctcg cccttgcacg ggcgagggat tcagcggtgc
    13621 ttgcagctcg aatcctgcgc gtccgggcgc ggtttcgggg gcgggcacgg ctcgatcgtg
    13681 ggaatgcgcg gcggccccgg cggctccgcg ggcatgcccg ggcagggggg cttgctgcgc
    13741 gacaggcgcg cgagatcgtc gcgcacgtgg ttcatctcgg tctggtaggc ggcgcgctcc
    13801 tcgttgagga ggtcgaccag cgtcgtcccc tcgcggaagt cggtgaagag cccgcgcagc
    13861 gacgcgaaga agccttcctt gtcccggtcg ttggtccact tctggcacac ggtgacgtgg
    13921 acgctttcgt gcgcgccgag tacctgcttc aggcaggcgg tgggattgcc cttgtaggtg
    13981 atgatgcagg cgttgttggt gtcggccgtg ggccggttcg cgttcgcgtc ggtgacctgg
    14041 ttgatggcct cctgcacgca gggctggacg tgctcggcgt aacgcgccac ggtgtacatc
    14101 acgggagcgc cggttttggt ctcctgggcg cggatcttgg cgatctgcct ggcgtattcg
    14161 ccgatggccg cgcgggcttc gcaataccgg ttgcgcaggt ccgcgaggtc ggcacaggcg
    14221 caggccgaac cctgcgcacg cgccgggccc gcgaccgcga aggcaaggag cagcgccacg
    14281 ggcgccccat tcaggatctt cagtctcgac atcatcattg cgctcgggcg gcacgccgct
    14341 tcagtagagt ttgtcatcgg aaaccggctg cgcctgggtt gcgggagcgg ccgatggcgc
    14401 gcttgcggga tcgagcgccg ccgcctcgac cttttcgcga gtgccatcgg gacgcacgcg
    14461 cagcatcagc ccgaagggct tcatgccgcc accgggcgcc tcgcgccagt cgcccgccag
    14521 cacctcgccg ttggggctgc ggcgcacgcc ccagccgacg atcgccggcc cccagaaccg
    14581 gccttcgaaa acggtgccgt cgggcatcgt gagcttgccg ggccctatcg ggcggcctcc
    14641 cgagaacgct gcctcgatgc ggcgcccgtc acgcgtgacg tgcagcccgg ggccgtacag
    14701 ggagccgctg tagaactgcc cttccatcag ctcgccgttg ggccatgcct tcctgccctt
    14761 gccgtgcagt cgcccctcca cgaacgtccc ttccgtgcgc gtgcagtcgg acgagatcga
    14821 cacgccctcg ccggaatcgg gcggctgctg gtaggtgcag cccttccccg gcacagtggt
    14881 cgcctggatg gccacgggcg cgggcgcggc gcgacggtgc aggaattgcc aggtgcccac
    14941 gcccagcgcg gcgagcagca atggcacgcc gaccagcacg ctccagaacg tggccatcgt
    15001 agcgccagag agcaattgcg attcggccgt cgacgcgtcg agcgtggatt gcgcttccgc
    15061 tcgcgcccgg tcgatggccg ccacgcgccc ggccggctcc gcgagttggg gagccgcgac
    15121 tttcgcctcg tacaacgcct gcccgaggct tccgagattg ccggagcggt ccttctgcac
    15181 ggcgctcgcc tgctgcgagg cgccttgcga ttccttgcgc acgcgggcga gattctcgtc
    15241 gatgtgcgcg atggatgcct tctgttccgc gtcgatcacc gcgatctccg cggcgtactt
    15301 ctggctctcg ccggagaggc ggctttcctc ggtcacgtgg gccggaaggt cctcgcgggc
    15361 cttcgccatc ctcgcgccga ggtcgacctg ctcggcggcg agcttcgcgc gctccgcctg
    15421 cgcggccgcg agcttgggac cttgcccggg tgctgcggca gttccgaaag ccgcgatgtc
    15481 cctgtcgagc gtggagagct tgccggcgat ggccgccagg cgcgactcgg actgcttgat
    15541 cgcttgttcg atcgcggtct tcgccgatcg cgacgtgcgc agggcggcgt cgatgggact
    15601 cttcttctcc tcgaccgcct tgcggcgggc ggcgaatttc tcgagctcgg cccggcgctc
    15661 ggtctcgagg gtggactttt ccgcgtcgag tttgctcgcc gtcgcggaga gctcgccggc
    15721 gcgggcgtcg agcccggtca ggcggtcgcg caactcggcg aaggccgaga ggtcgatctt
    15781 ttcctcccag gcgcgctggc cgagggcgac gagggctgcg gcgcggtccg tctcctgctt
    15841 gcgcatgagg ctgcgcagct tggcgcgatc gatcttgcgt cccagctccc ggaaaccctc
    15901 cgacaggaaa ttgccgcgct tcggttccgt cgcgtttcgc tcttccatca atcccggctt
    15961 tcgtcctgac attcgcattg ttgattgacg acgctctgcc ggcgcctttc ggggaatttc
    16021 cgcgtttcag tcgttgcggc atttccggat ggtccgatat tgaatgggga gagcgcgcgt
    16081 gtctacttcg gcggacacaa tttccgcgga acttccgctc gggcgtgcct gcgccacgat
    16141 cggaattgcg gaacgtgatt gccgcatgaa gcgctttccg acgaccagcc ggaaagccat
    16201 ttagcggata gccgaattgc cagttggcgc ttggccgctt accaaaattg cgatcagcac
    16261 gttgagccgc ctgtagccgc cggccggtcc gacttatcca cattccgcga acccgtcacg
    16321 cacggtcatg ttcccggccg tttccatgcc tacaatgatc gtttgaccgc agacgaagcc
    16381 cattccccca ggcttcctgc atccctgccg attcaacaaa cgacccagga acccctccat
    16441 gcggcaattc ttcaatttca tcgccggcgc ggccctgctg ctggcccttt ccggctgcgc
    16501 gcccctcctg acccagagcc cgggcagggg gctgagcccc gtcaacgccg tctccgatgg
    16561 cggggacgag cacctcatcc tcttcggcca cgacgtggtc agctacttca ccgaaggcac
    16621 gcaccagcgc ggcttgcccg cgatcaagtc cgtgtacaag ggcgtcacat tccgtttcgc
    16681 gagcccggag cacaaggcgc tcttcgacca gtcgccggag cgatacctgc cgcagttcaa
    16741 cggctactgc gccaacggca tggtctacgg catcccctgg ggcggcgacg ccgacacgtg
    16801 gaagatgatc gacggcaagc tctacatctt cggcggcgcc gggtcgcgcg acgcgttcct
    16861 gctcgacgtg cccaggaaca tggcgctcgc ccaccggtac tggaaccagg aagtgaacgg
    16921 gtcgaacgcc ttcgtgcagc gcgcctggcg cctggtggtc cgcgtgccgc attacaagag
    16981 cggcgcgcag ctcgccgagg aagtggccaa ggcgaagcgg tgaagtcgtg aacgaacttt
    17041 cgcaagtccc cggcatcgat cccggccccc tcgacgcgga atccggcggc gcgatatcca
    17101 ttcgcggcgc ccgccagaac aacctcaaga acctcgatgt cgacatcccc acgggcgagc
    17161 tcgtcgtcgt gaccggccca tcgggctcgg gcaagagctc gctcgtcttc gatacgctct
    17221 acgccgaggg ccagcgccgc tacgtcgaga cgttttcgcc ctacgcgcgg cagttcctcg
    17281 accgcatgga caagccgcag gtggactcga tcgagggcat cccgccggcc atcgccatcg
    17341 accagaccaa tccggtgcgc acctcgcgct cgacggtcgg cacgatgacg gagctgaacg
    17401 accacctgaa gctgctcttc gcccgcgccg gccacctctt ctgccgcaat tgctcgcgcc
    17461 ccgttcgccg cgactcgccc gagtcgatct tcgccgagat cgccgaacgc gccaaaggcg
    17521 cggacgaccc gcgcgtcgcg atcaccttcc ccatcgaggt gccgaagaat ttcaccgagg
    17581 aggaagtcac caggcacctc gaggcgcagg gctatgcgcg catccaccgg cgcaagggcg
    17641 cgcgactgga cgtcgtgcag gaccgcctgc gcctgggcaa cgcggagaaa gcccgggtcg
    17701 tggaggcgat cgaggcggcg ctgcgcgtgg gcgccgggag ggtgaacgtg ttcgtcgagg
    17761 acagtgccga tgcgtggcgc ttctccacgg acctgcactg ccccgattgc gacatccact
    17821 attccgaccc gagccccgcg cgcttctcct tcaactcgcc cgtcggcgcc tgcgacaagt
    17881 gccgcggttt cgggcgcgtg atcggcgtgg actacgggct ggtcgtcccc gacgaggcga
    17941 agtcgctcgg caacggcgcg gtgaagcctt tccagaccgc gagtttcgac gagtgccagg
    18001 acgacctcga gaagtacgcg aagcgtcgcg gcgtcgccct cgacatcgcc tggcgcgacc
    18061 tgcccgccga gcaccggcgc tgggtcatcg agggagacga ggactggatc ggctggaaga
    18121 agacgggcaa gacgcactgg tacggcgtgc agcgcttctt cgactggctc gagaccaagg
    18181 cgtacaagat gcacatccgg gtcctgctct ccaggtaccg cgcctacacg ccgtgcgcgg
    18241 actgcgacgg ggcgcggctg aagcccgagt cgatgctctg gcgcgtcggc acgaagaccg
    18301 aggcggacgc ggtgctggcc ccggaaaggc ggtacccgcc gcggaacacg aagtggaacc
    18361 tcgatgagct cgccgcgttg ccgggcctgt gccttcatga cgtgatgctc ctgccgatcg
    18421 agcgcgcgcg aaccttcttc gagcgcctcg ccctgccggc gcccatggac gaagccgccg
    18481 agctgctcct cgaggaggtc aacgcgcggc tgcggttcgt ctgcgaggtg gggctgggct
    18541 acctcacgct cgaccgccag tcgcgcacgc tttccggcgg cgaggtgcag cgcatcaacc
    18601 tcaccacggc gctcggcacc tcgctcgtga acacgctctt cgtgctggac gagccctcca
    18661 tcggactgca cccgcgcgac atgggccggg tgatcggcgt gatgaagcgg ttgcgcgatg
    18721 ccggcaactc gctcgtggtg gtcgagcacg acccgcagat catgctggaa gccgaccgcc
    18781 tgctcgacat ggggccgggg cccggcgagc gcggcggcga gatcgtcttc ttcggcacgc
    18841 ccgatgggct gcggcaggcg aagaccgtca ccgccgagta cctctccggc cgccgccgcg
    18901 ccgacgcggg agtcgtctcg caaccgcccg ccgccgacac gccgcgcctc gcgatccgcg
    18961 gtgtcaccga gcacaacctg aagggcatcg acgtcgagat cccgctgcat cgcttcgtgt
    19021 gcgtgacggg cgtgtcgggc tccggcaagt ccacgctgat ccaggacgtc ctgcatccgg
    19081 cgctcctcaa gcatctcggc aagccgaccg agtcaccggg cgcattccgc gagatgcacg
    19141 gcgcggggcg aatctccgac gtcgtgttcg tcgaccagtc gcccatcggc cggacgacgc
    19201 gctccaatcc cgccagctat gtcggcgcct tcgacgccat ccgcgccctg ttcgcgaaga
    19261 cgcaggatgc gaaaggccgc ggctacacgg cgggcacctt cagcttcaac tcgggcaacg
    19321 ggcgctgccc cacctgcagc ggcaacggct tcgagcacgt ggagatgcag ttcctgtccg
    19381 acgtgtacct gcgctgcccg gactgcaacg ggcgccgcta ccgcgacgag atcctcgagg
    19441 tgaagtgggg aggacgaaac atcgcggagg cgctggagct cacggtgcac gaggcgctcg
    19501 acttcttcgc gggggagccg gacgtagtgg cgcggttgcg gcccctggcc gacgtgggtc
    19561 tcgactacct gaggctgggg cagccggtgc cgacgctgtc gggcggcgag gcgcagcgac
    19621 tgaagctcgc gggccacctc atcgaggcct cgggccggcc ggtggcgatg ctgccgcgac
    19681 gccctgccga cggcatcgcc ccggcgctcc atcccgacgt tgcgccggca cggacgcgcc
    19741 tcttcctctt cgacgagccc accacgggcc tgcacttcga cgacgtggcc aagcttctgg
    19801 ccgccttccg ccgcctcatc gccgcgggcc actccctcgt cgtgatcgag cacaacctcg
    19861 acgtgatccg cgcctgcgac tggctcatcg acctcggccc cgaaggcggc gacgctggcg
    19921 gcgacatcgt cttcgccggc actccatcgg atgcgacact ccatcccgcc tcccacaccg
    19981 gaaaagctct cctcgattac gacaaggccc tccattccac cgaccatctg cgttcatccg
    20041 tgttcatctg tggttcccaa gatttgcgtt cgaattactc tccgacgcat cctttctcga
    20101 agaacatcca ggtccgccac gcccgcgagc acaacctcaa gaacatcgac gtcgacatcc
    20161 cgcgcggcaa gttcaccgtg atcaccggcg tctccggctc cggcaagtcc accctcgcct
    20221 tcgacatcct cttcgccgag ggccagcgcc gctacctcga gtcgctcaac gcctacgcac
    20281 gccagttcgt gcagccggcc tccaagcccg acgtggacgc gatcttcggc atcccgccca
    20341 cggtggcgat cgagcagcgc accagccgcg gcggctcgaa gagcacggtg gccacgcaga
    20401 ccgagatcca ccatttcctg cgcctgctgt tcgtgaagct gggcacgcag cattgccccg
    20461 actgcgacgt gcccatcgag ccgcagagcg aggaagcgat cgccgcgcgc ctgttccgcg
    20521 agcaccgcgg cgagcgcatc gcgctcctcg cgccgctggt ggtgagccgc aagggctatt
    20581 acaccgatct cgccaagtgg gccgggtcga agggcgtatc gcacctgcgc gtcgacggcg
    20641 agtacctgtc caccggccgc tggccgcggc tggaccgctt ccgcgagcac acgatcgaga
    20701 tgcccgtggc ggaagtggaa gtgacgcccg ccaacgaggt cgcgctgcgt gaggcgatcg
    20761 ccgccgccgt cgagcacggc aagggcgtgg tgcacgccct tcccggcgga tggggcgcga
    20821 cgcattccgt gaacttctcc acgaagcgcg cgtgccgttc ctgcgggcgc agcttccccg
    20881 aacttgaccc gcgcctcttc tcctacaact caaagcacgg ctggtgcccc gcctgctacg
    20941 gcacgggcgt gaagctcgag ggctcgggca tcgactggaa cgacgagcgc gcgaagaccg
    21001 gcacggagga ccacgtgctc gattcgtggg tctcgtggct cgaactcgac gaagcctgcc
    21061 cggcgtgcgc gggccggcgg ctcaatcgcg aggcgctggc cgtgcgcttc cgcggtcaat
    21121 ccatcgccgc gatgagcgcc cagccgatcg aggcggcgca gcgcttcttc cgcggactcg
    21181 ccttgacggg gagagaccag gaaatcgcgc gcgatatcct tgccgaactc gcctcgcgcc
    21241 tttcgttcct cgagcaggtg gggctgggct acctgtcgct cgaccgcggc gcgccgaccc
    21301 tttccggagg ggaggcgcag cgcatccgcc ttgccgcgca gctcggatcg aa
//
LOCUS       DASUEV010000084         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_24362, whole genome shotgun sequence.
ACCESSION   DASUEV010000084 DASUEV010000000
VERSION     DASUEV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24362"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFV55_02655"
     CDS             complement(<1..207)
                     /locus_tag="VFV55_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX4943224.1"
                     /translation="MARMKFICDAERCIECNGCVTACKSEHEVPWGVNRRRVVTLNDG
                     VPGERSVSVACMHCSDAPCMAVCPT"
     gene            complement(239..3163)
                     /locus_tag="VFV55_02660"
     CDS             complement(239..3163)
                     /locus_tag="VFV55_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX4943225.1"
                     /translation="MLLNRKSEGRAERANPLSRAMNALGATTVDRRAFLKGSGITAGA
                     AAFASQLPLNMIGEAKAATGGGKIEVKRTVCTHCSVGCAVDAHVQDGVWIRQEPVFDS
                     PLNLGSHCAKGASVREHGMTEHSHRLKSPMKLVDGKYRKITWEQAINEIGDKLLAIRK
                     ESGPDAVFWLGSSKHSNEQSYMFRKFVSMFGTNNMDHQARICHSTTVAGVANTWGYGA
                     MTNSYNDLMNSKAMFFIGSNAAEAHPVSMLHALHAKETGARMIVADPRFTRTAAKADQ
                     YIRFRSGGDVPLLFGMLHHIFKNGWEDKQYLNDRVFGMEKVREEVMKNWTPDKVTEAC
                     GVPEAEVFQAAQSMAMNRPSTVVWCMGQTQHSIGNAMVRASCILQLALGNVGVSGGGT
                     NIYRGHDNVQGATDVGPNPDSLPGYYGIVAGSWKHWCRVWGVDYEWMKGRFASQAMME
                     KPGMTLSRWIDGVMEKNELIDQENNLRAVIYWGHAPNSQTRGLEMKTAMEKVDLMVIV
                     DPYPTASAVMPDRKEGVYLLPAATQFETSGTATASNRSIQWREKVVEPLFESKPDHTI
                     MYAFAKKFGFGAEFVKNIKLVKDKSGWDEPLVEDILREINAGVWTIGYTGQSPERLKA
                     HMRHMDAFDPKTLRCTKSVVDKETGYDLEGDYFGLPWPCYGNAALKHPGSPNLYDTSK
                     HVMDGGGNFRANFGISRDGVSLLAEDGSHSKGADITTGYPEFDHVLMKKLGWWGELTP
                     EEAKEAEGKNWKNDLSGGIQRVVLKNHGCHPFGNAKARAIVWNFPDGIPKHREPIYSN
                     RPDLVAKYPTHDDKKAFWRLPTLYKTAQQKNITDKVHEKYPIVLTSGRLVEFEGGGEE
                     TRSNPWLAELQQENFVEINTKDAQARGIKYWDYVWVSSPTGARIKVRALVTERVGQGV
                     SWIPFHFAGWWQGKDLLDKYPDGAAPIVRGEAVNTATTYGYDSVTMMQETKTTLCQIE
                     RA"
     gene            complement(3175..3372)
                     /locus_tag="VFV55_02665"
     CDS             complement(3175..3372)
                     /locus_tag="VFV55_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX4943226.1"
                     /translation="MNNHTKPNSRRKFLLTASLGGGGAAAAVVTGRKIAAGGEVAPAA
                     EQPRGYHVSEHILKYYKTTQV"
     gene            complement(3401..>3914)
                     /locus_tag="VFV55_02670"
     CDS             complement(3401..>3914)
                     /locus_tag="VFV55_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX4943227.1"
                     /translation="KLLRTLALAAPLEGGDGALADAWKALVAASAVMDEEAVAEEYET
                     LFVGVGKAKVSVYAGWYTGAHAVDHPRVRIIEDLAALGLGHPAGIAEPEDHFAGLLDA
                     MRILIAGGAGRAPSGIAEQKRFYELHLKPGVGAFLAALREAPESNYYRKVAAVGEAFV
                     GIENESFNLG"
BASE COUNT          571 a         1298 c         1285 g          760 t
ORIGIN      
        1 agtcgggcag actgccatgc atggcgcgtc cgaacagtgc atgcaggcca ccgacaccga
       61 gcgctcaccg ggcacgccgt cgttgagggt cacgacgcgc cgtcggttca cgccccaggg
      121 cacctcgtgt tcgctcttgc acgccgtcac gcagccattg cactcgatgc agcgctcggc
      181 gtcacagatg aatttcattc tggccatttg tctttctcct aatatccgtg gcggtgcgct
      241 aggcgcgctc gatctggcac agggtggtct tggtctcctg catcatcgtc accgagtcgt
      301 agccgtaggt ggtggccgtg ttcacggcct cgccgcgcac gatcggcgcc gcgccatccg
      361 ggtacttgtc gaggaggtcc ttgccctgcc accagcccgc gaaatggaac gggatccagg
      421 aaacgccctg gccgacccgc tccgtgacca gggcgcgcac cttgatgcgc gcgcccgtcg
      481 gggagctgac ccagacgtag tcccagtact tgatgccgcg agcctgggcg tccttcgtgt
      541 tgatctccac gaagttttcc tgctgcagct cggcgagcca cgggttggag cgcgtttcct
      601 cgccgccgcc ctcgaactcg accaggcgcc cggaggtgag cacgatcggg tacttctcgt
      661 gcaccttgtc ggtgatgttc ttctgctgcg cggtcttgta gagcgtgggc aggcgccaga
      721 atgccttctt gtcgtcgtgc gtcggatact tggcgacgag gtcggggcgg ttggagtaga
      781 tcggctcgcg gtgcttcggg atcccgtcgg gaaagttcca gacgatcgcc cgcgccttcg
      841 cgttgccgaa cgggtggcag ccgtggttct tgaggacgac gcgctggatg ccgccggaaa
      901 ggtcgttctt ccagttcttg ccttcggcct ccttcgcctc ctcgggcgtg agctcgcccc
      961 accagcccag cttcttcatg agcacgtggt cgaactcggg atagcccgtg gtgatgtcgg
     1021 cgcccttgga gtgcgagccg tcctcggcca gcagggagac gccgtcacgc gagatgccga
     1081 agttggcgcg gaaattcccg ccgccgtcca tcacgtgctt ggaggtgtcg tagagattcg
     1141 gcgagcccgg gtgcttgagc gcggcgttgc cgtagcaggg ccacggcagg ccgaagtagt
     1201 cgccctcgag gtcgtagccc gtctccttgt cgacgacgga cttcgtgcag cgcagcgtct
     1261 tcggatcgaa ggcgtccatg tggcgcatgt gggccttgag gcgctccggg gactggccgg
     1321 tgtagccgat ggtccacact cccgcgttga tctcgcgcag gatgtcctcc acgagcggct
     1381 cgtcccagcc cgacttgtcc ttcaccagct tgatgttctt cacgaactcc gcgccgaagc
     1441 cgaacttctt ggcgaacgcg tacatgatcg tgtggtccgg cttggactcg aagagcggct
     1501 cgacgacctt ttcgcgccac tggatcgagc ggttcgacgc ggtggccgtt ccggaggtct
     1561 cgaactgcgt ggccgcgggc aggaggtaga cgccttcctt gcggtcgggc atcactgccg
     1621 aagcggtggg atacgggtcg acgatcacca tcaggtcgac cttctccatc gccgtcttca
     1681 tctccaggcc gcgcgtctgc gagttgggcg cgtggcccca gtagatgacc gcgcgcaggt
     1741 tgttttcctg gtcgatgagc tcgttcttct ccatcacgcc gtcgatccag cgcgagaggg
     1801 tcatgcccgg cttctccatc atcgcctggg aagcgaagcg ccccttcatc cactcgtagt
     1861 cgacgcccca gacgcggcac cagtgcttcc acgaccccgc gacgatgccg tagtagcccg
     1921 gcagcgagtc ggggttgggg cccacgtcgg tcgcgccctg cacgttgtcg tggccgcggt
     1981 agatgttggt cccgccgccg gagacgccga cgttgccgag tgccagttgc aggatgcagc
     2041 tcgcgcgcac catcgcattg ccgatggagt gctgggtctg ccccatgcac cacacgacgg
     2101 tcgagggccg gttcatggcc atggactgcg cggcctggaa gacctcggcc tcgggcacgc
     2161 cgcaggcttc cgtcaccttg tccggcgtcc agttcttcat cacctcctcg cgcaccttct
     2221 ccatcccgaa gacgcggtcg ttgaggtact gcttgtcttc ccagccgttc ttgaagatgt
     2281 ggtggagcat gccgaagagc agcggcacgt cgccgccgga acggaagcgg atgtactggt
     2341 cggccttcgc cgcggtgcgc gtgaagcgcg ggtcggcgac gatcatcctc gcgcccgtct
     2401 ccttggcgtg cagcgcgtgc agcatcgaga ccgggtgggc ctcggccgcg ttcgacccga
     2461 tgaagaacat ggccttcgag ttcatcaggt cgttgtagga gttggtcatc gccccgtagc
     2521 cccacgtgtt cgcgacgccc gcgacggtgg tggagtggca gatgcgcgcc tggtggtcca
     2581 tgttgttcgt gccgaacatc gacacgaact tgcggaacat gtaggactgc tcgttgctgt
     2641 gcttggagga gcccagccag aaaacggcgt cggggcccga ttccttgcgg atcgcgagca
     2701 gcttgtcgcc gatctcgttg atcgcctgtt cccaggtgat cttgcggtac ttgccgtcga
     2761 ccagcttcat cggcgacttc aggcggtgcg aatgctcggt catgccatgc tcgcgcacgg
     2821 aggcgccctt ggcgcagtgc gagccgagat tgagcggcga atcgaacacg ggttcctggc
     2881 ggatccacac gccgtcctgg acgtgcgcat cgacggcgca acccacggaa cagtgggtgc
     2941 agaccgtgcg cttcacctcg atctttccgc cgcccgtggc ggccttcgcc tcgccgatca
     3001 tgttgagcgg caactggctc gcgaacgcgg ccgcgccggc ggtgatgccg gagcccttca
     3061 ggaaggcgcg gcgatcgacg gtcgtcgccc cgagggcgtt catggcgcgg gaaagcgggt
     3121 tggcgcgctc ggcccggcct tcggatttgc gattgagcag catggtcggc ctcctcagac
     3181 ttgcgtggtc ttgtagtact tcaggatgtg ctcggagacg tgatagcccc gcggctgttc
     3241 ggcagccggg gcgacttccc cgcccgcggc gatcttcctg cccgtcacga cggccgcggc
     3301 cgcgccgccg ccaccgaggc tcgcggtgag caggaacttc ctgcggctgt tcggtttggt
     3361 gtggttgttc atgtcacgcc tcctgttcag taactgcaat tcaacccagg ttgaacgact
     3421 cgttttcgat gccgacgaaa gcctcgccga cggctgcgac cttccggtag taattcgatt
     3481 cgggcgcttc gcgaagggcc gcgaggaatg cacccacgcc cggcttcaag tgcaactcgt
     3541 agaagcgctt ctgttccgcg atgccggaag gcgcgcgccc cgcaccgccg gcgatgagga
     3601 tccgcatcgc gtcgagcagg cccgcgaagt ggtcttccgg ctccgcaatt cccgccgggt
     3661 ggccgagccc gagcgccgcg agatcctcga tgatgcgcac ccgcgggtga tcgacggcgt
     3721 gcgcccccgt gtaccacccc gcgtagaccg acaccttcgc cttgcccacg cccacgaaca
     3781 gcgtctcgta ttcctccgcg acggcctcct cgtccatcac ggcggaggcc gcgacgagcg
     3841 ccttccacgc atcggcgagc gcgccgtcgc ccccttccag gggagcggcg agcgccagcg
     3901 tccgcaggag cttc
//
LOCUS       DASUEV010000085        10423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_482653, whole genome shotgun sequence.
ACCESSION   DASUEV010000085 DASUEV010000000
VERSION     DASUEV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482653"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFV55_02675"
     CDS             complement(<1..188)
                     /locus_tag="VFV55_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4943228.1"
                     /translation="MCTEASSRRQAGISLVELVMFIVVVGIGVAGILSVLNVTASRSA
                     DPLPVKQALAIAESLLEEV"
     gene            complement(178..618)
                     /locus_tag="VFV55_02680"
     CDS             complement(178..618)
                     /locus_tag="VFV55_02680"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX4943229.1"
                     /translation="MQRNRGFTLIELVVVLVLVGILAVFVAPRFDTAVFSTRGFADQV
                     GAALRFAQKTAVAQRRTVCVTTTSSSLTLTQAAAPPPSAACGGMLAIPGTKAGILNAP
                     AGVTLSVVTLHFTALGQAVPGTTFTVTGDAARTITIEGETGHVH"
     gene            complement(903..1274)
                     /locus_tag="VFV55_02685"
     CDS             complement(903..1274)
                     /locus_tag="VFV55_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4943230.1"
                     /translation="MNLKSQQGFTLIELVVVIVILGILAAVAVPKFIDLSGEASQAAV
                     EGVAGGLSSASAVNYAARKANAAKGIAIANCTDVAGALQGGLPTGYVITAGAVVADAT
                     ATCTLTGQNAKTATFTALGIS"
     gene            complement(1329..1805)
                     /locus_tag="VFV55_02690"
     CDS             complement(1329..1805)
                     /locus_tag="VFV55_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4943231.1"
                     /translation="MRTRQAGFTLMELVIVIVIIGILAAVALPRFVNMQRDARTAKAQ
                     GIYGAVKSAAVLAKSRCELDLSRLVAGGTCTATGGTVNMDGAVVSMVNRYPAATASGI
                     DRAAQVSPAEGVTIGGSGSQRIYEVAGAGLAVECRIVYNEAPLGGAPDIQIVTSGC"
     gene            complement(1914..3143)
                     /locus_tag="VFV55_02695"
     CDS             complement(1914..3143)
                     /locus_tag="VFV55_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4943232.1"
                     /translation="MPQFAYKGRNARGEAVDGVLDSATAASVADQLMAGGVTPVEIKP
                     GRPAETGGDVDRSLFRPKVGPMDVLLFSRHLYTLLKAGVPIMSALGGLRDSATNAAFR
                     ELLADVQESLDAGRELSAALGRHAKVFTPFYVAMVRVGEMTGMLHEIFLRLFHYLDFE
                     RVMRDQVKAALRYPTFVVIAIAVAIVIVNIFVIPPFAKVYQGFNAKLPLMTELLIGFS
                     GFMVTFWPVLLGFAVAMVMGFRAWTATVAGRLAWDEMKLRLPISGSILKKAALARFAR
                     SFSLSARSGVPIVQAMGLVAATVGNSYMGGKVERMREGIERGESVLRTAAASGIFTPV
                     VLQMIAVGEESGALDDLMQEVADMYQREIEYDLKNLSAQIEPVLIIFLGVLVLILALG
                     VFLPIWDLGRAAMGRPT"
     gene            complement(3149..4855)
                     /locus_tag="VFV55_02700"
     CDS             complement(3149..4855)
                     /locus_tag="VFV55_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX4943233.1"
                     /translation="MTGPAKVRLGEVLVEQGLLTKAQLDSALAEQKQSARKLGRIIVE
                     RGLVPEEAISRALGKQLGIPFVDLRHFNVRREIVRRLGENQARRLRAVVLDDRDSVIR
                     VGLADPTNLAAYDELLRILGKNVELAVVTESDLLATIDRIYQRTEEISTLAKELTQEV
                     GDFSIDFGGLLDTTPRGEDAPVVRLLQTIFEDAVRVRASDIHIEPQEKTLNIRFRIDG
                     VLHLQIESDMKIASALVQRLKLMAGLDISERRLPQDGRFNVKVRNNPVDVRMSTMPTQ
                     WGESVVMRLLTQAGGVLGLERLGMPPRILERLRHTIHRTGGMVLVTGPTGSGKTTTLY
                     AMLSEINTPEKKIITVEDPVEYRLPGINQVQVHDRIELTFERVLRSALRQDPDVIMVG
                     EIRDRVTAETGLRAAITGHMVFSTLHTNDAVGTPIRLLDMGVPRYMVAMSLQLVVAQR
                     LVRVNCVACAEEYVPDAAEMTWLGEHRLDEADRPSFRRGRGCSHCNGTGFRSRIGIFE
                     MLEMTRPVVEAANHEDPVTFVRVAREQMGDRTLRNHAARLVLAGKTTAREAMRVDSEL
                     EE"
     gene            complement(5065..5757)
                     /gene="bioD"
                     /locus_tag="VFV55_02705"
     CDS             complement(5065..5757)
                     /gene="bioD"
                     /locus_tag="VFV55_02705"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX4943234.1"
                     /translation="MSRGVFVTGTDTGVGKTVVACALVHALRARGYRVAPMKPVAAGA
                     ISREGVWANEDTLALAQAAGPDAPAHHAITPVLLREAMAPHIAASREGLRIDLAALVE
                     AHRQLALRADFTVVEGVGGFRVPLGPSLDARDLASALQLPVVLVVGLRLGCLNHALLT
                     AESIERAGLALAGWIANAIDPAMRAQDENVDALAERLRAPLLGRLPFAPDHAPATRAG
                     FLDVKPLVDTRG"
     gene            complement(5754..6617)
                     /gene="bioC"
                     /locus_tag="VFV55_02710"
     CDS             complement(5754..6617)
                     /gene="bioC"
                     /locus_tag="VFV55_02710"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEX4943235.1"
                     /translation="MLPVKRDVRRAFQRAARTYDEAAVLQREVCTRLLEHLDPIRLVP
                     QRAVDLGCGTGQAFDALAKRFPGATIVGLDLAPAMLARARERSPWWQRVLGSPRPALV
                     CADAERLPLANASVDLAFSNLALQWCEPSRVFAEAARVLGAEGLFLFSTFGPDTLREL
                     RAAFAEADVAPHVNRFIDMHDLGDALVDAGFADPVMEMETITLEYDTVLAIARDLKAI
                     GAVNSLPSRSRGLPGRNRWRRMTQAYEQFRRNGVLPATWEVIYGHAWKVPPKRLADGR
                     QVVSFAPKAPR"
     gene            complement(6622..7383)
                     /gene="bioH"
                     /locus_tag="VFV55_02715"
     CDS             complement(6622..7383)
                     /gene="bioH"
                     /locus_tag="VFV55_02715"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEX4943236.1"
                     /translation="MTLHVERLGAGPDLVLLHGWGLHGGVWHSLAARMAGSFRVHLVD
                     LPGHGHSHGVPFAGLDAVVDAVSPCAPEGAVICGWSLGGLVAIRLATRHPSRAAALAL
                     VSTTPCFVQRPGWPHAMQRTTLEAFAEGLRKEPAKTIRSFVNLNALGGPQSRDRMREL
                     AALLAGRGTPSAESLHAGLALFHDADLRDEVATIDRPAAVIHGGRDALTPVGAGRWLA
                     GALPAARFVEIADAAHLPFVSHTEAVAQALEALRG"
     gene            complement(7367..8539)
                     /gene="bioF"
                     /locus_tag="VFV55_02720"
     CDS             complement(7367..8539)
                     /gene="bioF"
                     /locus_tag="VFV55_02720"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX4943237.1"
                     /translation="MEQAGLAALARGLDDLGRAGLLRARRVLDSAQGPVVLEGGQPLA
                     NFASNDYLGLANHPRLRAAAHAAIDAYGFGAGSSPIVVGHSRLHEEAQAAFARFTALP
                     RALMFPSGYAANLGILTALADRSAEIFADKLNHTCLNDGALLSRAAFHRYAHGDLERL
                     EAMLVASKSAIRVIATDTVFSMDGDIAPLPDLLSLAERYDAWLVLDDAHGIGVLGEGR
                     GSLAHFRLRSPRIVYMATLGKALGGYGAFVAGEAVVIEWLTQRARTYVFSTALPPVVA
                     AVASESLRLIEEHPALVGTLHERIGEFRRACTRHGIETASSTAIHPIVVGDATRAVVL
                     SARLRELGQLVPAIRPPTVPDGMARLRVSLSAAHSRDQVLALAAALGEVLHDDAAR"
     gene            complement(8547..9467)
                     /gene="bioB"
                     /locus_tag="VFV55_02725"
     CDS             complement(8547..9467)
                     /gene="bioB"
                     /locus_tag="VFV55_02725"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX4943238.1"
                     /translation="MRDEARALLDLPFNDLLFKAQTVHRENFDPNRIQRSTLLSIKTG
                     GCPEDCGYCPQSARYATGVENEAMLPLDEVVRSAQAARENGATRFCMGAAWRGPKARD
                     LEPVLDMVREVKKLGLETCCTLGMLKDGQAEQLREAGLDYYNHNLDTAPEFYGEVITT
                     RDYGDRLATLERVRGAGIRVCCGGIVGMGESRDARAGLLAQLASLDPQPESVPINQLV
                     QVEGTPLAGTEALPWTEFVRTIAVARILMPKSFVRLSAGRTQMHEAVQALCLLAGANS
                     IFYGEKLLTTGNPDVERDDALFEALGLTAG"
     gene            9563..10309
                     /locus_tag="VFV55_02730"
     CDS             9563..10309
                     /locus_tag="VFV55_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX4943239.1"
                     /translation="MPAVKWFVEHFRTVAQKTGSIRELKFPWPADCLLCLDAVRGAMV
                     CDNCERLLPNSRRRCPRCALPMSEDWWCADCLREPPAFDDVVTAFDYRFPIDRLVRRF
                     KFSADLATGAYLGDALARAAATAPMPDLVLASPASAARLRERGFNPALVLARRVASAA
                     GIAVDSRVVAKIRHTPPQTGLDHATRRRNLRGAFAVRRRLDGIHVAVVDDVMTTGTTL
                     SALAAVLKAAGAARVSGWVAARTPEPPRED"
     gene            10314..>10423
                     /locus_tag="VFV55_02735"
     CDS             10314..>10423
                     /locus_tag="VFV55_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831517.1"
                     /note="member of the SPOUT superfamily of RNA
                     methyltransferases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX4943240.1"
                     /translation="MVDVVLVNPEIPPNTGNVIRLCANTGTPLHLVAPLG"
BASE COUNT         1674 a         3536 c         3597 g         1616 t
ORIGIN      
        1 acttcctcca gcagcgactc ggcgatggcg agcgcctgct tgaccggcag cgggtccgcg
       61 ctgcgcgacg cggtgacgtt cagcaccgaa aggatgccgg ccacgccgat gccgacgacg
      121 acgatgaaca tgaccagctc gaccagcgag atgcctgcct ggcgccggct cgacgcctca
      181 gtgcacatgg cccgtttcac cctcgatggt gatggtgcgc gcagcgtcgc ctgtgacggt
      241 gaaggttgtg ccgggaaccg cctgcccgag cgccgtgaaa tgaagggtca ccaccgacaa
      301 ggtgacgccc gcgggcgcat tcaggattcc ggcttttgtg cccggaatgg cgagcatgcc
      361 cccgcaagcc gcgctgggcg gcggcgccgc ggcctgggtc agcgtgaggg aactcgatgt
      421 agtcgtgaca cagacggtgc gccgctgtgc gacggccgtc ttttgcgcga aacgcagcgc
      481 cgccccgacc tggtcggcga atccgcgcgt gctgaagacg gcagtgtcga agcgcggcgc
      541 gacgaagacg gccaggatcc ccacgagaac gaggacgacg accagttcga tcagggtgaa
      601 accgcgattg cgttgcatcg gccgcagttc cttctccggg gagcgattcc ccgtgaatca
      661 cccgccgcga cacggcgcca atgcgcatgg ccgtacgaac gacagtaatc gccttgccgg
      721 caacggaacc cctttcggga ccggtgccgg caccaggtgg gcgctttcga cggcggaacc
      781 gtgccctctt ccaccattac cacggacggg cacggattac gacaacgacc tatacgcctg
      841 gcgcccgcac cgatgcatcg aaaccaaaac gctccgcggc tggcaggccg gggtcgcgaa
      901 tatcaggaaa ttcccagcgc cgtaaaggtg gccgtcttcg cgttctgtcc ggtcaaggtg
      961 caagtcgccg tggcgtcggc aacgaccgct cccgcggtga tcacgtatcc cgtgggcagc
     1021 ccgccctgca aggcgcctgc gacatccgtg caattggcga tcgcgatgcc cttggccgcg
     1081 tttgccttcc gcgccgcgta attcaccgcc gaagccgaag acaggccgcc tgccacgccc
     1141 tccacggctg cctggctcgc ttcacccgag agatcgatga atttcggcac cgcgaccgct
     1201 gccaggatgc cgagaatcac gatgaccacg accagttcga ttagcgtaaa gccttgctgg
     1261 gatttcaagt tcattttgct tcctctagga gttctgcatc caattgcatc cgggtgccca
     1321 tgggcttttc agcacccgct cgtgacaatc tgaatatccg gcgcaccgcc cagtggcgcc
     1381 tcgttgtaga caatacgaca ttctacagca aggcccgctc cagccacttc gtagatccgc
     1441 tgcgagcccg agccgccaat ggtcacgcct tccgccggcg acacctgcgc ggcgcggtct
     1501 atcccggagg ccgtggccgc gggatagcgg ttgaccatgc tgaccacggc gccgtccatg
     1561 ttcaccgtgc cgcccgtcgc cgtgcacgtc ccgccagcca cgagccggga caggtccagt
     1621 tcacagcgcg acttcgcgag gaccgccgcc gacttgaccg caccgtagat gccctgcgcc
     1681 ttggccgttc gcgcatcgcg ttgcatgttg acgaagcgcg gcagcgcgac ggcggcgagg
     1741 atgccgatga tcacgatcac gatcaccagc tccatcaggg tgaatccggc ttggcgggtt
     1801 cgcattctct gtccggaagg caacgggatt tccaaccggc atgtttatca atcaatggta
     1861 cttgttaatc accgggagga ccacgtggcg ccggaaaacg tgcccgcccc ccatcaggtg
     1921 ggccggccca tcgccgcgcg gccgaggtcc cagatcggca ggaatacgcc gagcgccagg
     1981 atgagcacga gcacgccgag gaagatgatg aggacgggct cgatctgggc gctcaggttc
     2041 ttcaggtcgt attcgatctc gcgctggtac atgtccgcga cttcctgcat caggtcgtcg
     2101 agggcaccgg actcctcgcc caccgcgatc atctggagca ccaccggcgt gaagatgccg
     2161 gaagccgccg cggtgcgcag cacgctttcg ccacgctcaa taccctcgcg catccgttcc
     2221 accttgccgc ccatgtagct gttgcccacc gtggccgcga cgaggcccat ggcctggacg
     2281 atcggcacgc cgctgcgcgc cgagagagag aagctccgcg cgaagcgcgc aagtgccgcc
     2341 tttttcagga tgctgcccga aatgggcagc cgcaacttca tttcgtccca cgcgagccgc
     2401 ccggcaacgg tggcggtcca ggcgcggaat cccatgacca tcgcgacggc gaagccgaga
     2461 agaaccggcc agaaggtcac catgaacccc gagaaaccga tcagcagttc cgtcatcagg
     2521 ggcagcttgg cgttgaatcc ctggtagacc ttggcgaagg gcgggatgac gaagatgttc
     2581 acgatcacga tggcgaccgc tatcgcgatc accacgaacg tcgggtagcg cagcgccgcc
     2641 ttcacctggt cgcgcatgac gcgctcgaaa tccaggtagt ggaacagccg caggaatatc
     2701 tcgtgaagca tgccggtcat ctcgccgacg cgcaccatgg cgacgtagaa cggcgtgaac
     2761 actttcgcgt gccggcccag cgccgcggag agttcgcggc ccgcgtcgag gctctcctgg
     2821 acgtccgcca gcaattcgcg gaacgcggca ttcgtggcgg aatcgcgaag cccgccgagg
     2881 gcgctcatga tcggcacgcc cgccttgagc agcgtgtaga gatggcggct gaacagcagc
     2941 acgtccatcg ggccgacctt gggccggaaa aggctccggt cgacgtcgcc ccccgtctcg
     3001 gccgggcggc cgggcttgat ctcgacgggc gtcacccctc ccgccatcag ttggtcggcc
     3061 accgaggcgg cagtcgcgct gtccaggacg ccgtcgaccg cctcgccgcg ggcgtttcgc
     3121 cccttgtagg cgaattgcgg cacgcggcct attcctccag ttcgctgtcg acgcgcatgg
     3181 cctcgcgcgc ggtggtcttt ccggcaagca caagtcgcgc cgcgtggttg cgcagcgtcc
     3241 ggtcgcccat ctgctcgcgg gccacgcgca cgaaagtgac cgggtcctcg tggttggcgg
     3301 cctcgaccac cggccgggtc atctcgagca tctcgaagat tccgatgcgc gaccggaacc
     3361 cggtgccatt gcagtgcgag cagcctcgtc cccgccggaa cgagggcctg tccgcctcgt
     3421 cgagccggtg ctcgcccagc caggtcatct ccgcggcgtc aggcacgtac tcctccgcac
     3481 aggccacgca attgacgcgc acgaggcgct gggccacgac gagctgcagc gacatcgcca
     3541 ccatgtaccg gggcactccc atgtcgagga ggcggatcgg cgtacccacc gcgtcgttcg
     3601 tgtgcagggt ggaaaacacc atgtggccgg tgattgccgc gcgcagtccc gtttcggccg
     3661 tgacccggtc gcgaatctcg cccaccatga tgacgtcggg atcctggcgc agcgcggagc
     3721 gcagcacgcg ctcgaaggtg agttcgatgc ggtcgtggac ctgcacctgg ttgatcccgg
     3781 gcaggcggta ttccaccgga tcctccaccg tgatgatctt cttctcgggg gtgttgatct
     3841 cggagagcat cgcgtagagg gtggtcgtct tgccgcttcc cgtcgggccg gtgaccagca
     3901 ccatgccccc cgtgcgatgg atggtgtgcc gcagccgttc gagtatgcgc ggcggcatgc
     3961 ccaggcgctc cagcccgagc acgccgcccg cctgggtgag caggcgcatc acgaccgatt
     4021 cgccccattg cgtcggcatg gtggacatgc gcacgtccac ggggttgttc ctgaccttca
     4081 cgttgaagcg gccgtcctgc ggcaggcggc gctccgaaat gtccagcccg gccatgagct
     4141 tcagccgctg caccagggcc gaggcgatct tcatgtccga ctcgatctgc aggtggagca
     4201 cgccatcgat gcgaaagcgg atgttgagcg tcttttcctg cggctcgatg tggatgtccg
     4261 aggcgcgcac gcgcacggcg tcctcgaaga tggtctgaag cagccggacg acgggggcgt
     4321 cttccccgcg cggcgtggtg tcgagcaatc cgccgaagtc gatcgagaaa tcacccacct
     4381 cctgggtgag ttccttggcc agggtggaga tctcctcggt ccgctggtag atgcgatcga
     4441 tggtcgcgag caggtccgac tcggtcacca ccgcgagttc cacgttcttg cccaggatgc
     4501 gcagcagttc gtcgtaggcg gccaggttgg tgggatcggc cagtccgacg cgaatgacgg
     4561 agtcgcggtc gtcgaggacc accgcgcgca gccgccgcgc ctggttctcg ccgaggcgcc
     4621 ggacgatctc gcgccggacg ttgaaatgcc ggaggtccac gaaggggatg ccgagctgct
     4681 tgcccagcgc cctggagatg gcttcctcgg gaaccagccc gcgctccacg atgatgcggc
     4741 cgagcttgcg ggcgctttgc ttctgttcgg cgagcgcgga atcgagctgt gccttggtga
     4801 gcaacccctg ctcgacgaga acctccccga gccgcacctt ggcgggcccc gtcacgaggt
     4861 ctctccggcg ttatgcagga tcattgtcga acgttggctc cggggcgccc cgattccggt
     4921 gaacagtctg cgcggcacgc gcttccgtcc cgcaccccaa tcacgcaatg atgtcagtcg
     4981 cgaagccgaa aggcaaagcg gcgtcgtgca cgtggcgggg gacgggatgg aaaatccgcc
     5041 tggccggggg cctgcgcaag gcactcagcc gcgcgtgtcg acgagcggtt tcacgtcgag
     5101 gaatcccgct cttgtcgccg gcgcgtggtc gggcgcgaac ggcaggcggc cgaggagcgg
     5161 cgcgcgcagc cgttcggcga gggcgtccac gttctcgtcc tgcgcacgca tggccgggtc
     5221 gatcgcattg gcgatccagc cggccagcgc gagaccggcg cgctcgatcg actcggcggt
     5281 aagcagcgcg tgattgaggc agcccagccg caggccgacg acgaggacga cgggcaattg
     5341 cagggcgctt gcgagatcgc gggcgtccag ggacggtccc agggggacac gaaacccgcc
     5401 cacgccctcg acgacggtga aatccgctcg caaggccagt tggcggtggg cctcgaccag
     5461 cgcggcgagg tcgattctca ggccttcgcg ggaagcggca atatgcggcg ccatcgcctc
     5521 gcgcagcaat accggcgtga tggcgtgatg cgccggcgcg tccggcccgg cagcctgcgc
     5581 gagcgctagc gtgtcctcgt tcgcccacac gccttcgcgc gatatcgcgc cggccgccac
     5641 gggcttcatc ggtgcgacgc gataaccccg ggcacgcagg gcgtgcacga gcgcgcaggc
     5701 gacgacggtc ttgcccaccc cggtgtcggt gccggtcacg aacacgccgc ggctcaacgc
     5761 ggggccttcg gcgcgaagct caccacctgc cggccgtcgg cgagccgctt cggaggcacc
     5821 ttccacgcgt gtccgtagat gacctcccag gtcgccggca gcacgccgtt gcggcggaat
     5881 tgctcgtagg cctgcgtcat gcgccgccag cggttgcgcc ccggcagccc gcgggagcgc
     5941 gacggcagcg agttgaccgc gccgatggcc ttcaggtcgc gggcgatcgc gaggacggtg
     6001 tcgtactcca gcgtgatcgt ctccatttcc atgacgggat cggcgaatcc cgcgtccacg
     6061 agcgcgtcgc cgaggtcgtg catgtcgatg aagcggttga cgtgcggggc aacgtcggcc
     6121 tcggcgaacg cggcccgcag ttccctgagc gtgtccggcc cgaaagtgga gaagaggaag
     6181 agcccttcgg cgccgagcac gcgcgcggct tccgcgaaga cgcgcgaagg ctcgcaccac
     6241 tgcagcgcga ggttggagaa ggcgaggtcg accgatgcgt tggcgagcgg cagccgctcc
     6301 gcgtccgcgc acaccagcgc cgggcgcggc gatcccagca cgcgctgcca ccatggcgat
     6361 cgctcgcgcg ctcgcgccag catggcggga gcaagatcga ggccgacgat cgtcgcgccc
     6421 ggaaagcgct tcgcaagcgc gtcgaacgcc tgcccggttc cgcagccgag gtccacggcc
     6481 cgctgcggga cgagacggat cggatcgagg tgctcgagaa ggcgcgtgca cacctcgcgc
     6541 tgcaggacgg cggcctcgtc gtaggtccgc gcggcgcgct ggaaggcccg gcgcacatcg
     6601 cgtttcaccg gaagcatcgc ctcatccacg cagggcctcg agagcttgcg ccacggcttc
     6661 ggtgtggctc acgaagggca ggtgggccgc atcggcgatt tcgacgaagc gcgcggcggg
     6721 aagcgcgccg gcgagccagc gtcccgcgcc tacgggcgtg agcgcgtcgc gcccgccatg
     6781 gatcacggcg gcggggcggt cgatcgttgc gacctcgtcg cgcaggtccg cgtcgtggaa
     6841 cagcgcgagc ccggcgtgta gcgattcggc ggagggtgtc ccgcgcccgg ccagcagcgc
     6901 ggccagctcg cgcatccggt cgcgcgactg tggccctccg agcgcattga gattcacgaa
     6961 gctgcggatc gtcttcgcgg gctccttgcg caggccctcg gcgaacgcct cgagcgtcgt
     7021 gcgctgcatc gcatgcggcc agcctggccg ttgcacgaag cacggcgtgg tgctcacgag
     7081 ggcgagagcc gcggcgcggg acgggtggcg cgtggcgaga cggatggcca cgagtccgcc
     7141 cagggaccag ccgcagatga ccgcgccctc cggcgcgcag ggcgacaccg cgtcgacgac
     7201 agcgtcgagc cccgcgaacg gaacgccgtg cgagtggccg tggcccggca ggtcgacgag
     7261 gtgcacgcga aaggagccgg ccatccgggc cgcgagggag tgccacacgc caccgtgcag
     7321 tccccacccg tgcagcagca cgaggtcggg accggcaccg agccgctcaa cgtgcagcgt
     7381 catcgtgaag cacctcgccg agggcggcgg ccagcgcgag cacctggtca cgggaatggg
     7441 ctgccgacag cgaaacgcga aggcgtgcca tcccgtccgg aaccgtgggc gggcggatcg
     7501 cggggacgag ctggccgagc tcgcgcagtc gcgcggaaag cacgaccgcg cgcgtggcgt
     7561 cgccgacgac aattgggtga atggcggtcg acgacgcggt ctcgatgccg tgccgcgtgc
     7621 aggcgcgacg gaactcgccg atccgttcgt gcaatgtgcc cacgagcgcg ggatgttcct
     7681 cgatcaggcg caaggattcg ctggcgacgg ccgcgaccac cggcggcaac gccgtcgaga
     7741 acacataggt ccgcgcgcgc tgcgtcagcc actcgatcac gacggcctcg cccgcgacga
     7801 atgcgccgta gccgccgagg gcctttccga gggtggccat gtacacgatg cgcggcgagc
     7861 gcaggcggaa atgcgccagc gagccgcggc cttcgcccag cacgccgatg ccgtgcgcgt
     7921 cgtcgagcac gagccacgcg tcgtaacgct cggcgagcga gagcaggtcg ggaagcggcg
     7981 cgatatcgcc atccatgctg aagacggtgt cggtcgcgat cacgcggatc gcggacttcg
     8041 aggcgacgag catcgcctcc agccgctcga ggtcgccgtg cgcatagcga tgaaaggccg
     8101 cgcgcgacag gagtgcgccg tcgttgaggc aagtgtggtt cagcttgtcg gcgaagattt
     8161 ccgcggagcg gtccgcgagc gccgtgagga tgccgaggtt ggcggcgtag ccgctcggga
     8221 acatgagcgc gcgcggcagc gcggtgaagc gcgcgaaagc cgcctgcgcc tcctcgtgca
     8281 gccgggagtg gccgacgacg atgggggacg agcccgcgcc gaagccgtag gcgtcgatgg
     8341 ctgcgtgcgc ggcggcgcgc aagcgtggat ggttggcgag gccgaggtaa tcgttggagg
     8401 cgaaattcgc gagcggctgc ccgccttcaa gcaccaccgg cccctgcgcc gaatcgagca
     8461 cgcggcgcgc gcgcagcagc cccgcgcggc cgagatcgtc cagcccgcgc gcgagagcgg
     8521 ccagtccggc ttgctccacg tcagcctcag ccggccgtga ggccgagcgc ctcgaagagc
     8581 gcgtcgtccc gctcgacgtc ggggtttccg gtcgtgagga gcttctcgcc gtagaagatg
     8641 gaattggcgc ccgcgagcag gcacagcgcc tggacggcct cgtgcatctg cgtgcggccc
     8701 gcggaaagcc ggacgaagct cttcggcatg aggattcgcg ccacggcgat cgtgcgcacg
     8761 aactccgtcc acggcagcgc ctcggtgccg gcgagcggcg tcccttccac ctgcacgagc
     8821 tggttgatgg gcaccgactc gggctgcggg tcgaggctcg cgagctgcgc gagaagaccg
     8881 gcgcgcgcat cgcgcgattc gcccatgccc acgattcccc cgcagcagac gcggatcccg
     8941 gcgccgcgaa cgcgctcgag cgtcgccagc cggtcgccgt aatcgcgcgt cgtgatcacc
     9001 tcgccgtaga actcgggcgc ggtgtcgagg ttgtggttgt agtagtcgag tcccgcttcg
     9061 cgcaactgct ccgcctgccc gtccttcagc atgccgagcg tgcagcaggt ttcgagcccc
     9121 agcttcttga cctcgcgaac catgtcgagg accggctcga ggtcgcgcgc cttgggtccg
     9181 cgccacgccg cgcccatgca gaagcgcgtg gcgccgtttt ccctggcagc ctgggccgag
     9241 cgcaccacct cgtccagcgg cagcatcgcc tcgttctcca cgccggtggc atagcgcgcc
     9301 gattgcgggc agtagccgca gtcctcggga cagcctcccg tcttgatcga gagaagcgtc
     9361 gagcgctgga tgcggttcgg atcgaaattt tcgcggtgga ctgtctgcgc cttgaagagc
     9421 agatcgttga agggaaggtc gaggagggcc cgcgcctcgt cacgcagcgt gcgggtgcgg
     9481 gggtccgccg gggcggcaag gtgaacgatc tggtccaagg ggcgcggcct gtgtttgtaa
     9541 gttgctgatg attaggaaga atatgccagc tgtcaaatgg ttcgtcgagc acttccggac
     9601 ggttgcccaa aaaacaggca gtatccgcga gttgaagttc ccgtggccgg ccgattgcct
     9661 gttgtgcctg gatgcggtcc gcggggcgat ggtatgcgat aattgtgagc gcttgctacc
     9721 aaattcccgc cgacgctgcc cgcgatgtgc cctacccatg tccgaggatt ggtggtgcgc
     9781 cgactgcctg cgcgagccgc ccgctttcga tgacgtcgtg actgcctttg actaccggtt
     9841 tccgatcgat cgcctggttc gccggttcaa gttctcggcc gacctggcca ccggcgccta
     9901 ccttggcgat gctctggcgc gtgccgcggc aaccgccccg atgccggatc tcgtcctcgc
     9961 ctcgccggca tctgccgcca gactgcggga gcgaggcttc aatcccgccc tggtgctggc
    10021 tcgccgggtg gcgtctgcgg caggcatcgc cgtcgactcc cgggttgtcg cgaagatccg
    10081 gcatacgccg ccacagacgg ggctcgacca cgcgacccgg cgccgcaact tgcgcggggc
    10141 gttcgccgtc cgccgccggc tggacgggat tcatgtcgcg gtcgttgacg acgtgatgac
    10201 gaccgggacc acgctttccg cgctcgccgc cgtgctcaag gcggctggtg ccgcgcgggt
    10261 gtccgggtgg gtggcggcgc gtacgcccga gccgccgagg gaggactgaa gccatggtgg
    10321 acgtcgtcct cgtgaacccc gagattccgc ccaatacggg caacgtgatc cgcctgtgcg
    10381 ccaacacggg cacgcccttg cacctggtcg cgcccctggg ctt
//
LOCUS       DASUEV010000086         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_826309, whole genome shotgun sequence.
ACCESSION   DASUEV010000086 DASUEV010000000
VERSION     DASUEV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826309"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1796
                     /locus_tag="VFV55_02740"
     CDS             354..1796
                     /locus_tag="VFV55_02740"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX4943241.1"
                     /translation="MLDALGRDFRAIAPAVDFCALRFVEESSELLTVRQDVAEPPQLS
                     TDRGAMVTVIDKGGLGYAATSDLTPAGLREAASRATRLAQLTAGRSVVDYGRIAMPSP
                     RGTYASPPGDDPARLTRREKLDLLAGEAKQCRIDERIVDWEASLWTTRTRQAYFTADG
                     GEVEQVFHYLVPNMSATAHANGITQTRSHAGRGNGYCRQGGLEILDSFGLKGSGRSVA
                     EGALELLAAPNCPGGKMDVLLMPDQMMLQIHESIGHPLELDRILGDERNFAGTSFVTL
                     DMFGSYRYGSELLDVTFDPARPEEFASYGWDDDGLAAERQFLIRKGILERPLGGAISQ
                     ARAGIAGVANTRACSWNRAPIDRMANLNIEPGRSSLDDLIAGIDQGVMMRTNISWSID
                     DSRNKFQFGCEWGRMIRNGRLAEVVKNPNYRGISATFWRSLSGVGDASTFEVMGTPFC
                     GKGEPNQVIRCGHASPACRFSGVDVFGGAA"
     gene            1799..3121
                     /locus_tag="VFV55_02745"
     CDS             1799..3121
                     /locus_tag="VFV55_02745"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX4943242.1"
                     /translation="MKSYFLELADFVQSLAAAGEVIASCFSAESSDFIRFNRSAVRQA
                     TAVRQFRWTLTLIRDGRRVDAAATLAGQGAADREQLKAMLADLRDGIGEAPVDPYLLY
                     PTAPVNTERESPGEIPDASLVIDAVLDAGRGLDLVGLYAGGPIHEGYANSLGQRNWQR
                     VDNFNFEWCLYHDRDKAVKTSYSGPRWEADALATRMRFAREQLALLGKPARTLDPGHY
                     RAYLSPAAMEDVLGMLCWGGFGLKSQRTKHSPLMRMADGAERFDPRVTICENTAEGLA
                     AGFQREGFVRPGRVELIRQGRPADALVSPRSAKEYGVATNGANGEETPESLDLAAGEL
                     PRAEVLETLDRGIYVGNLWYLNFSDRSACRLTGMTRFASFWVEDGEIRAPLNVMRFDD
                     SAFRMLGSELEALTRERDLMLQGGTYGSRNTSSMRTPGALLRDFALVL"
BASE COUNT          543 a         1189 c         1106 g          539 t
ORIGIN      
        1 aggcgaacgt gaacggagac ggtgggtggg gtccgattgg ctgcttcgaa tttttctttc
       61 cggccagatc aacttgctaa cagcatcgac ttcggggaaa cctcgacata ggttcgatcc
      121 gtgacgcccg acgcaacttg acctggccgg aaagaagaat tcgaagcagc caatcggacc
      181 ccacccaccg tctccgttca cgttcgcctc caactggcac atcgtctatc tcgttcgaag
      241 atagcggagt gaggccggtg cggcgcgtcc aggtgacgcg gcgcgcgacg ttcgggcgta
      301 aaatgcgttc tggccgccca cgaggcggcc gcttcttttc ccccggagcc cccatgctcg
      361 atgccctcgg gcgcgacttc cgcgccattg cccccgccgt cgatttctgc gccctgcgct
      421 tcgtggagga atcctcggag ttgctcaccg tgcgccagga cgtggccgag ccgccgcagc
      481 tctccaccga ccgcggcgcg atggtcacgg tgatcgacaa gggcggcctg ggctacgcgg
      541 ccacgagcga cctcacgccc gcgggcctgc gcgaggccgc gagccgcgcc acgcgcctcg
      601 cgcagctcac cgcggggcgc agcgtcgtgg actacggcag gatcgcgatg cccagcccgc
      661 gcggcaccta cgcctcgccg ccgggcgacg atcccgcccg gctcacgcgg cgcgagaagc
      721 tcgacctcct ggcgggcgag gcgaagcagt gccggatcga cgagcgcatc gtggactggg
      781 aagcgagcct gtggaccacg cgcacgcgcc aggcttattt caccgccgat ggcggcgagg
      841 tggagcaggt cttccactac ctcgtgccca acatgtcggc cacggcgcat gccaacggca
      901 tcacgcagac gcgctcccat gccggccggg gcaacggcta ctgccggcag ggcgggctgg
      961 aaatcctcga ttccttcggc ctcaagggca gcggccgctc ggtcgcggaa ggggcgctcg
     1021 aactgctcgc cgcgccgaac tgcccgggcg ggaagatgga cgtcctcctc atgccggacc
     1081 agatgatgct gcagatccac gagtccatcg ggcacccgct ggagctcgac cgcatcctgg
     1141 gcgacgagcg caatttcgcc ggcaccagct tcgtcacgct ggacatgttc ggctcctatc
     1201 gttacggatc ggaactcctg gatgtgacct tcgatccggc gcgccccgag gaattcgcaa
     1261 gttacgggtg ggacgacgac gggctcgccg ccgagaggca gttcctcatc cgcaagggca
     1321 tcctggagcg cccgctgggc ggggcgattt cgcaggcgcg cgccgggatc gcgggcgtag
     1381 ccaacacgcg cgcttgctcg tggaaccgcg ctcccatcga ccgcatggcc aacctgaaca
     1441 tcgaaccggg ccgctccagc ctcgacgatc tcatcgccgg catcgaccaa ggcgtgatga
     1501 tgcgcacgaa catctcgtgg tccatcgacg actcgcgcaa caagttccag ttcggctgcg
     1561 agtggggccg catgatccgc aacggcaggc tcgcggaagt ggtgaagaat ccgaactacc
     1621 gcggcatctc cgccaccttc tggcgctcgc tctccggtgt cggcgacgca tccacgttcg
     1681 aggtgatggg cacgcccttc tgcggcaagg gagagcccaa ccaggtcatt cgttgcggcc
     1741 atgcctcccc ggcctgccgg ttctccggcg tggacgtctt cggcggagcg gcatgatgat
     1801 gaaatcctat tttctcgagc tcgccgattt cgtgcagtcg ctggccgccg ccggcgaggt
     1861 gatcgcctcc tgcttctcgg ccgaatcctc ggacttcatc cgcttcaaca ggagcgcggt
     1921 gcgccaggcc actgccgtgc gccagtttcg ctggacgctc acgctcattc gcgacggccg
     1981 ccgcgtcgac gccgcggcca cgctggccgg ccagggcgcc gcggaccgcg agcaactgaa
     2041 ggcgatgctc gccgacctgc gcgacggcat cggcgaagcg cccgtcgacc cgtacctgct
     2101 ctacccgact gcgccggtga acaccgagcg tgaatcgccg ggcgaaattc cggacgcgtc
     2161 gctcgtgatc gatgcggtgc tcgatgccgg tcgcggactg gacctcgtcg gcctctacgc
     2221 gggcgggccc atccacgagg gctatgccaa ttcgctgggc cagcgcaact ggcagcgcgt
     2281 ggacaacttc aatttcgaat ggtgcctgta ccacgatcgc gacaaggcgg tgaagacttc
     2341 gtactcgggg ccgcgctggg aggccgacgc gttggccacg cgcatgcgct tcgcccgcga
     2401 acagctcgcc cttctcggca agcccgcgcg cacgctcgac ccgggccatt accgcgccta
     2461 cctctcgccc gcggcgatgg aggacgtgct ggggatgctc tgctggggcg gcttcgggct
     2521 caagagccag cgcaccaagc acagccccct catgcgcatg gcggacggcg ccgagcgctt
     2581 cgacccccgc gtgacgatct gcgagaacac ggccgagggg ctcgccgccg gcttccagcg
     2641 cgagggcttc gtgcgcccgg gccgcgtgga attgatccgg caggggcgac cggccgacgc
     2701 gctcgtttcg ccgcgttccg cgaaggagta cggcgtggcg accaacggcg ccaacgggga
     2761 ggaaacgccc gaatcgctcg atctcgccgc cggcgaactt ccgcgcgcag aggtgctcga
     2821 aaccctggac cgcggcatct acgtgggcaa cctctggtat ctcaacttct ccgaccgctc
     2881 ggcctgccgc ctcaccggca tgacgcgttt cgcctcgttc tgggtggagg acggcgagat
     2941 ccgcgcgccg ctcaacgtga tgcgcttcga cgacagcgcc ttccgcatgc tcggttccga
     3001 actggaggca ctcacgaggg agcgcgacct catgctgcag ggcggtacct atggatcgcg
     3061 caacacttcg tcgatgcgca cgcccggggc cctgctgcgc gacttcgccc tcgtcctcta
     3121 gccggattga cgccgtgccc tcggcggcgt agcatccaaa aaacccatag gaaacaataa
     3181 ttaagcttat ggcgtggaag cgaaatctca gggggatggc gttgcttgcc gcgctggcgg
     3241 ccatcggcgc ccagggagcc gatgcgcccc ggcccccgct tgctcccgcg gcgatcccgg
     3301 acgcgttcga cgaccccgat ctcccgatgc cggacccgga cctgtggcag cgcgtgcgca
     3361 agggcttcct gctcgag
//
LOCUS       DASUEV010000087         8659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_941130, whole genome shotgun sequence.
ACCESSION   DASUEV010000087 DASUEV010000000
VERSION     DASUEV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941130"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VFV55_02750"
     CDS             <1..1393
                     /locus_tag="VFV55_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4943243.1"
                     /translation="VMAQLHELHRAGHTIILITHDAAVAAEADRTIQLRDGVVVDDRR
                     HAHAPPPATGKAPHALPVAQTSLLPDVLEAVKMAMRSLRTNLFRTVLTLLGIIIGVGA
                     VVAMLALGDGSKAAVLSRIEAMGTDLLIITPGARNVRTRGEKDNLVAEDAEAIADLPN
                     VREALAQYGSGVTVRYGGVDYTTECTGTTANFARVHDWTPTQGSFFAEADVKGFAPVA
                     VLGQTVADNLFRNGEEPIGKYVLINNIPFQVIGVLAPKGANAAGKDLDDNMFVPLSTA
                     RMRLFGKRYVYPITVQVADMAHAQETEAAITALLTERHRKVDFQIRNMASLLEAASET
                     QNTLTLLLGSIAAISLLVGGIGVMNIMLVSVTERIREIGIRMATGARMLHILLQFMTE
                     ALVVCAVGGAVGVAGGLAAAWIAGRLGSPVIFSLAPVVMAFGSAFLIGLLFGFLPARR
                     AARMDPVVALATE"
     gene            1426..2805
                     /locus_tag="VFV55_02755"
     CDS             1426..2805
                     /locus_tag="VFV55_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX4943244.1"
                     /translation="MAILLTVAIAGCATQADRYERPAVDTPARWSGRAGTDAWPDRNW
                     WSAFGSPELDALIGEAQAANHDVRAAAARVGQARANAGLASAGLFPVLSLGFDGQRSK
                     DPGKSAVNSYALGPQASYEVDLWGRLRDTARAGDAALLASEFDRETVRLGLTADVANA
                     YFQLLSLNDRIRVAEENVVVARRLLDLLSVQQQAGRVSTLEVERQRSLVASTEAAIPP
                     LRQQRVVTRDALAVLLGRPLDKTPDPKGSLRTLAPPAARGGVPAQLVERRPDIRRAEA
                     DLMAANANVGAARGALFPNLTLAANGGTQAGTVAALFGSGTGYYALAAALVGTIFDGG
                     ARRARVDLSQAQREERVEFYLQAVVSSFRDVEDALAGIEQFAVQEDLLVTAAKSAREA
                     YRIVDVRYREGAENFFNVLDAQRTLLSAESAVDQARLARFAAATGLYRALGGGWDGRT
                     ETPLAQATR"
     gene            complement(2844..2933)
                     /locus_tag="VFV55_02760"
     tRNA            complement(2844..2933)
                     /locus_tag="VFV55_02760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2897..2899),aa:Ser,seq:cga)
     gene            complement(2987..3520)
                     /locus_tag="VFV55_02765"
     CDS             complement(2987..3520)
                     /locus_tag="VFV55_02765"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX4943245.1"
                     /translation="MEIVLVVMVVFAFVVVAWLTLAQSGEPPRPGKAAPDFRLQGIDG
                     KEQSLSALRGTRAVIVFHPQDETPECVAVVERLAAAAPAMASAGAWLATVVVSRREAA
                     QAYAGTHAAGLRVLCDADGRAAKAYGALVNLGFMKFARKLIVLVDARGNVERVWRDAV
                     GSEQVDELLGVFAGPPR"
     gene            3605..4945
                     /locus_tag="VFV55_02770"
     CDS             3605..4945
                     /locus_tag="VFV55_02770"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4943246.1"
                     /translation="MTKILHRSLRGSLPFAVSAKGMRFTDREGREYIDACGGAAVSCL
                     GHGHPDVLAAMHAQIDRVAYAHTSFFTTEVAEELADTLIADAPAGMSHVYLVSGGSEA
                     MEAALKMARQYCVESGQPERSVFIGRRQSYHGNTLGALAVGGNEWRRKQFAPLLIDVA
                     RVSPCYEYRDRREGESPEAYSERLAAELEATIAQVGPKRVIAFVAETVVGATAGAVPP
                     TPGYFRRVREICDGHGILLICDEVMCGMGRTGTLYATEQEGIVPDLVAIAKGLGGGYQ
                     PIGAVLAQARIVEAFRTGSGLFQHGHTYLGHPVAAAAALAVQKVIKRDNLLAAVRTRG
                     AYFERRLREALGGHPHVGDIRGRGLFRGVELVEDRATKRPFAPALKLHAKVKAEAMGR
                     GLMVYPMGGTIDGVNGDHVLLAPPFIATEADLDEIVGRLAAAIETSTAAIRRAA"
     gene            4981..5949
                     /locus_tag="VFV55_02775"
     CDS             4981..5949
                     /locus_tag="VFV55_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX4943247.1"
                     /translation="MKRITLGIALAAFAAFAAPDATAQQKYVTIGTGGVTGVYYAAGG
                     AICRLVNKDRAKHGIRCSVESTGGSVFNINTIKAGELDLGVTQSDVMYNAVKGLGQFK
                     DAGAFGELRAVMSLHPEPFTVVARKEANVKAFTEFKGKKFNVGNPGSGTRASMEELLG
                     AMGWKLGDFALASELKADEHGPALCDGKIDGFFYAVGHPSANIQDPTTSCGAKLVSLT
                     GPAVDKLVAEKPYYAKVTIPAGLYPNNPQATETYGVLAALVSSTKVPADSVYAVVKAV
                     FDNFEEFKKLHPALAHLKPEDMVKNGLSAPLHDGAARYYREKGWIK"
     gene            6030..8621
                     /locus_tag="VFV55_02780"
     CDS             6030..8621
                     /locus_tag="VFV55_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX4943248.1"
                     /translation="MDKDKKPVEPSAELQQLVADADTGGREVGGAVGKLILLVAVGWS
                     LFQLWYASPLPFVLGWGILNDTEARSLHLGIALFLGYLCFPALKSSGRASVPWYDWIL
                     AIAGAFAGAYFLLFYAELATRPGQPNLRDIVVGCAGLVMLLEATRRAVGLPMTVLAIV
                     FLVYILFGRYMPDVLAHKGASLERMISHQWLTTEGVFGVALGVSSSFIFIYVLFGALL
                     DRAGAGNYMMQVSMAFLGHMRGGPAKVAVVSSGLNGLISGSSVSNVVSGGIFTIPLMK
                     KAGYGGVKAGAIETMSSVNGQIMPPVMGAAAFLMVEYVGIPYTEICKNAFLPATLSYI
                     SLFYIVHLEALKLGLQPFAPGRARTMRERFIGWGLGLSGTIAVLCAIYFAAEAAKSLF
                     GAAAPWVMGFLLAALYVWCLRVAAKCPDLPKDIDVKNPVLPEGWPTVKAGMHFLIPFG
                     ILIWCLMIEELSPALAAFWATATLLVLMVTQRPLTELFRGRPIALREIKDGLREVFFG
                     FNDGARSMIGIAIATGTAGIIVGGITLTGLGLRMTDFVEFVSQGNVIVMLLFTAFVCL
                     VLGLGVPTTANYILVATLMAPVIVELGAESGLVIPLIAVHLFVFYFGIMGDITPPVGL
                     ASFAAAAISGEDPIQTGLQGSVYALRTVVLPFIWIFNPQLLLLDLEGWQELVYVVVAS
                     TVACLTFAAATMGWFQVKSRWWETAILLLATLMLFRPNFFMDQLYEPWESRPATALYQ
                     IATELPEDSRIVLVIEGTNVEGDDVRKTISVQLTKPGEGRARFAEAGVTFSVLGDETR
                     IGTVKFGSRAKRGGFEQGWKVREIKVRSDAPSEHWVFIPGFALIALVFYLQRRRMRPA
                     GAAPAMA"
BASE COUNT         1358 a         3041 c         2883 g         1377 t
ORIGIN      
        1 ggtgatggcg cagctgcacg agctgcaccg cgccgggcac acgatcatcc tgatcaccca
       61 cgacgcggcg gtcgcggccg aggccgatcg caccatccag cttcgcgacg gcgtggtcgt
      121 cgacgaccgg cgccacgccc atgcgccgcc gccggcaacg ggcaaggcgc cacacgccct
      181 gcccgttgcg caaacgagcc tgctgcccga cgtcctcgag gccgtgaaga tggccatgcg
      241 gtcgctgcgc accaatctct tccgcaccgt gctcaccctg ctcggcatca tcatcggcgt
      301 gggcgcggtg gtggccatgc tggcgctcgg cgacggcagc aaggccgcgg tgctctcgcg
      361 catcgaggcg atgggcaccg acctgctgat catcacgccc ggcgcgcgca atgtgcgcac
      421 gcgcggcgaa aaggacaatc tggtcgccga ggatgcggaa gcgatcgcgg acctgcccaa
      481 cgtacgcgag gccctcgcgc aatacgggag cggcgtcacg gttcgctacg gcggcgtcga
      541 ctacacgacc gagtgcacgg gaacgaccgc caacttcgcc cgcgtccacg actggacgcc
      601 gacgcagggc agcttcttcg ccgaagcgga cgtgaagggt ttcgcccccg tcgccgtgct
      661 cggccagacg gtggcggaca atctgttccg caatggcgag gagccgatcg gcaagtacgt
      721 cctaatcaac aacattccgt tccaggtgat cggcgtgctc gccccgaagg gcgcgaatgc
      781 cgccggcaag gacctggacg acaacatgtt cgttcccctg agcaccgccc gcatgcgcct
      841 cttcggcaag cgctatgtgt acccgatcac ggtgcaggtc gccgacatgg cgcacgccca
      901 ggaaaccgag gccgcgatca cggccctgct cacggagcgt caccgcaagg tggatttcca
      961 gatccgcaac atggcctcgc tgctcgaggc cgcgagcgag acccagaaca ccctgaccct
     1021 cctgctcggg tcgatcgccg ccatctccct gctcgtgggc ggcatcggcg tgatgaacat
     1081 catgctggtg tcggtgaccg agcgcattcg cgagatcggc atccgcatgg caacgggcgc
     1141 ccgcatgctg cacatcctgc tgcagttcat gaccgaggcg ctggtcgtct gcgcggtcgg
     1201 cggcgcggtc ggcgtggcgg gcggtcttgc cgcggcgtgg atcgccggcc gactgggttc
     1261 gccggtgatt ttctcgctcg cccccgtcgt gatggcattc ggcagcgcat tcctcatcgg
     1321 actgctgttc ggcttcctgc cggcgcgaag ggccgcgcgc atggaccccg tcgtcgccct
     1381 cgccacggag taagcaccat gtcgcccacc tgcccccggc ttccaatggc catccttctc
     1441 accgtcgcaa tcgccggctg cgcgacccag gccgatcgtt acgaacgccc tgcggtcgac
     1501 acgccggctc gctggtccgg tcgtgccggc accgacgcct ggccggaccg caactggtgg
     1561 tccgcattcg gcagcccgga actcgatgca ttgatcggag aagcgcaggc ggccaatcac
     1621 gacgtccgtg cggccgcggc acgggtcggg caggcgcgcg ccaatgccgg cctcgccagc
     1681 gccggtttgt ttccggtgct gagcctcggc ttcgacgggc agcgcagcaa ggacccggga
     1741 aagagcgcag tgaacagcta cgcgctgggc ccgcaggcaa gctacgaggt cgatctctgg
     1801 ggcaggctgc gcgataccgc gcgtgccggc gacgctgctc ttctggccag cgaattcgac
     1861 cgcgagacag ttcgcctcgg tctcaccgcc gacgtggcca atgcctactt ccagctgctc
     1921 tcgctgaacg accgcattcg cgtcgccgag gagaacgtcg tggtggccag gcggctgctc
     1981 gacctgctct ccgtccagca gcaggccggc cgcgtgtcga cgctcgaagt cgaacgccag
     2041 cgttcgctgg tcgccagcac cgaggcggcg atcccgccct tgcgccagca gcgcgtcgtg
     2101 acacgcgacg cgctcgcggt gctcctcgga cgccccctgg acaagacacc ggatccgaaa
     2161 ggctccctgc gcaccctggc accgcccgcg gcccgcggcg gcgttcctgc gcaactcgtc
     2221 gagcggcggc cggacatccg gcgcgccgaa gccgacctga tggccgccaa cgccaatgtg
     2281 ggcgcagccc gcggcgcgct gtttccgaac ctgacgctgg cggcgaacgg agggacccag
     2341 gcgggcacgg tcgccgccct gttcggctcc gggacgggtt actacgcgct cgccgcggcc
     2401 ctggtcggta cgatcttcga cggtggagcg cggcgtgccc gcgttgacct gtcgcaggcg
     2461 caacgcgagg agcgcgtgga attctatctg caggccgtcg tgtcctcgtt ccgcgacgtc
     2521 gaggacgcgc tcgcgggcat cgaacaattc gccgtgcagg aagacctcct ggtgacggcg
     2581 gcgaagtccg cccgcgaggc ttaccggatc gtcgatgtgc gctaccgcga gggtgccgag
     2641 aacttcttca acgtcctcga cgcgcagcgc accctgctga gtgccgagtc ggccgtcgac
     2701 caggcgcggc tggcgcgatt cgccgccgct acgggcctgt accgggcgct ggggggcggc
     2761 tgggatggcc ggaccgagac gccgctcgcc caggccacgc gatgaaaaaa gccaatccct
     2821 tgcggggatt ggcttgaact gactggcgga gagggtggga ttcgaaccca cggtaccccg
     2881 gagggtactt cagatttcga gtctgacgcg atcgaccact ctgccacctc tccggacccg
     2941 aagggcgcga ttatagccga ccggggtgcc gatttcgacc ctggactcag cgcgggggcc
     3001 cggcgaacac gccgagcagc tcgtccacct gctcggaacc cactgcatcg cgccagacgc
     3061 gctcgacgtt gccccgggcg tcgaccagga cgatcagctt gcgcgcgaac ttcatgaacc
     3121 ccaggttcac cagcgctccg taggccttgg cggcgcgacc gtccgcatcg caaaggaccc
     3181 gcaatcccgc cgcatgggtg ccggcatagg cctgcgccgc ctcccgccgg ctcaccacta
     3241 ccgtcgccag ccacgcgccc gccgaagcca tcgccggcgc cgcggcggcc aggcgttcga
     3301 ccaccgccac gcattccggc gtttcgtcct ggggatggaa gacgatcacc gcgcgggtgc
     3361 cgcgcagcgc cgagagcgat tgctccttcc cgtcgatccc ctgcaggcgg aaatcgggag
     3421 cggccttgcc gggccgcggc ggctcgcctg actgggcgag cgtgagccac gccacgacga
     3481 cgaaggcgaa gacgaccatg acgacgagga cgatttccac ggcggaagtc cggagttggg
     3541 cgggcgcagt gtagcagcgc cgagtcagga gcgccctcgc ggtgctatcc ttgcgcagtt
     3601 tcccatgacc aagatcctgc accggagcct gcgcggtagc ctgcccttcg cggtatcggc
     3661 caagggcatg cgcttcaccg atcgcgaggg tcgcgaatac atcgacgcct gcgggggcgc
     3721 ggccgtttcc tgcctcggcc acgggcaccc cgacgtgctc gccgcgatgc acgcgcagat
     3781 cgaccgcgtg gcctacgcgc acaccagctt cttcaccacc gaagttgccg aggaactggc
     3841 ggacacgctc atcgcggacg cgccggcggg catgtcgcac gtctatctcg tctccggcgg
     3901 gtcggaggcc atggaggccg cgctcaagat ggcgcgccag tactgcgtcg aatcggggca
     3961 gccggagcgc agcgtcttca tcggccggcg ccagagctat cacggcaaca cgcttggggc
     4021 gcttgccgtg ggcggcaacg aatggcggcg caagcagttc gcgccgcttc tcatcgacgt
     4081 cgcacgcgtc tcgccgtgct acgaataccg cgaccgccgc gagggcgaat ccccggaggc
     4141 ctacagcgag cgcctggccg ccgaactgga agcgacgatc gcgcaggtcg gaccgaaacg
     4201 cgtcatcgca ttcgtggccg agaccgtcgt cggcgcaacc gcgggcgccg tgccgcccac
     4261 gccgggctat ttcaggcgcg tgcgcgagat atgcgacggc cacggcatcc tgctcatctg
     4321 cgacgaggtg atgtgcggca tgggccgcac gggcacgctc tacgcaacgg aacaggaagg
     4381 gatcgtgccg gacctcgtgg ccatcgccaa gggcctgggc ggcggctacc agccgatcgg
     4441 cgccgtgctc gcgcaggcga ggatcgtcga ggccttccgc acgggcagcg gccttttcca
     4501 gcacggtcat acctatctcg ggcacccggt tgccgcggcg gcggcgctgg cggtgcagaa
     4561 ggtcatcaag cgcgacaacc tgctcgcggc ggttcgcacg cgcggtgcgt atttcgaacg
     4621 gcggctccgc gaggcgctgg gcggtcaccc tcacgtaggc gacatccgcg gccgcggact
     4681 cttccgcggc gtggaactgg tcgaggaccg cgccacgaaa cgcccgttcg cccccgccct
     4741 gaagctgcac gcgaaggtca aggccgaggc gatggggcgc ggcctcatgg tctacccgat
     4801 gggcggcacc atcgacggcg tgaacggcga ccacgtgctg ctcgcgcccc ccttcatcgc
     4861 cacggaagcc gacctcgacg agatcgtcgg gcggctcgcc gcggccatcg agaccagcac
     4921 cgcggccatc cggcgcgcgg cgtaggtatg acccaccgac ccaccaagag gagtccagca
     4981 atgaaacgaa tcaccctcgg catcgccctg gccgcgttcg cggccttcgc cgcgccggac
     5041 gcgaccgcgc agcagaaata cgtcaccatc ggcacgggcg gcgtcaccgg cgtgtactac
     5101 gccgcgggcg gcgcgatctg ccgcctcgtg aacaaggacc gcgccaagca cggcatccgc
     5161 tgctcggtgg aatccaccgg cggctccgtg ttcaacatca acaccatcaa ggccggcgag
     5221 ctcgacctcg gcgtgaccca gtccgacgtg atgtacaacg ccgtcaaggg gctcggccag
     5281 ttcaaggacg ccggtgcctt cggcgagctg cgcgccgtga tgtcgctgca cccggagccg
     5341 ttcaccgtgg tcgcgcgcaa ggaagccaac gtcaaggcct tcaccgaatt caagggcaag
     5401 aagttcaacg tcggcaaccc cggctccggc acccgcgcct cgatggagga actgctgggc
     5461 gccatgggct ggaagctcgg cgacttcgcg ctcgcctcgg agctcaaggc cgacgaacac
     5521 ggccccgccc tgtgcgacgg caagatcgac ggtttcttct acgcggtggg ccacccctcc
     5581 gccaacatcc aggacccgac cacgagttgc ggggcgaagc tggtctccct cacgggcccg
     5641 gccgttgaca agctcgtagc cgagaagccg tactacgcga aggtcacgat tcctgccggg
     5701 ctctacccga acaacccgca ggccaccgaa acctacggtg tcctcgccgc actggtgagc
     5761 tccaccaagg tgcccgccga cagcgtgtac gcggtcgtga aggcggtgtt cgacaacttc
     5821 gaggagttca agaagctgca cccggcgctc gcccacctga agcccgagga catggtgaag
     5881 aacgggctgt cggcccccct gcacgacggt gcggcgcgct actacaggga aaagggctgg
     5941 atcaagtgag agccccccgg catccgccgg ggtgacgcac ctgcggggtg ggcgttcgcc
     6001 cgccccgttt tcgcttgagg ggaggagccg tggacaagga caagaaaccc gtcgaaccca
     6061 gcgccgagct gcagcaactg gtggctgatg ccgataccgg gggccgcgaa gtcggcggcg
     6121 ccgtcggcaa gctgatcctc ctcgtggccg tcggctggtc gctcttccag ctgtggtacg
     6181 cctcgcccct gcccttcgtg ctcggctggg gcatcctcaa cgacaccgag gcacgctcgc
     6241 tccacctcgg catcgcgctg ttcctgggct acctctgctt tcccgccctg aagagttccg
     6301 ggcgcgcgag cgttccctgg tacgactgga tcctcgcgat cgccggcgcg ttcgccggcg
     6361 cctacttcct gctgttctac gccgagctcg ccacccgtcc gggacagccc aacctgcgcg
     6421 acatcgtggt cggttgcgcg ggcctcgtca tgctgctcga ggccacgcgc cgcgccgtgg
     6481 gactgccgat gacggtgctc gccatcgtgt tcctcgtcta catcctgttc ggcaggtaca
     6541 tgccggacgt gctcgcgcac aagggcgcct cgctcgagcg catgatctcg caccagtggc
     6601 tcacgaccga aggcgtcttc ggcgtggcgc tcggcgtgtc gtcctcgttc atcttcatct
     6661 acgtcctgtt cggtgcgctg ctcgacagag ccggcgccgg caactacatg atgcaggtgt
     6721 cgatggcctt cctcggccac atgcgtggcg gaccggcgaa agtggcggtc gtctcctccg
     6781 ggctcaacgg cctcatctcc ggctcctcgg tctcgaacgt cgtgtccggc ggcatcttca
     6841 cgatcccgct gatgaagaag gcgggctatg gcggcgtgaa ggccggtgcc atcgagacga
     6901 tgagctcggt caacgggcag atcatgccgc cggtgatggg cgcggccgcg ttcctgatgg
     6961 tggagtacgt cggcattccc tacacggaga tctgcaagaa cgccttcctg ccggcgacgc
     7021 tctcctacat ctccctcttc tacatcgtgc acctcgaggc gctcaagctc gggctgcagc
     7081 ccttcgcgcc cggccgcgcg cgcacgatgc gcgagcgctt catcggctgg gggctgggcc
     7141 tttccggaac gatcgccgtg ctttgcgcca tctatttcgc ggcggaggcg gcgaagtccc
     7201 tgttcggcgc ggcggcgccc tgggtaatgg gcttcctgct tgccgcgctt tacgtgtggt
     7261 gcctgcgggt cgccgcgaaa tgcccggacc tgccgaagga catcgacgtg aagaacccgg
     7321 tgctgcccga gggctggccg acggtgaagg ccggcatgca cttcctcatc ccgttcggaa
     7381 tcctcatctg gtgcctgatg atcgaggagc tctctcccgc gctcgccgcc ttctgggcca
     7441 ccgcgacgct tctcgtgctc atggtcacgc agcgccccct caccgagttg ttccgcggcc
     7501 gccccatcgc cctgcgcgag atcaaggatg gcctgcgcga ggtgttcttc ggcttcaacg
     7561 acggcgcccg aagcatgatc ggcatcgcca tcgcaaccgg caccgccggc atcatcgtcg
     7621 gaggcatcac gctcaccggc ctggggctgc ggatgaccga cttcgtggag ttcgtctcgc
     7681 agggcaacgt gatcgtcatg ctgctcttca cggcgttcgt ctgcctcgtc ctggggctgg
     7741 gcgtgccgac gaccgccaac tacatcctcg tggcgacgct gatggcgccg gtgatcgtgg
     7801 aactgggcgc cgagtcgggg ctggtgatcc cgctcatcgc ggtgcacctg ttcgtcttct
     7861 acttcgggat catgggcgac atcacgccac cggtggggct tgcgagcttc gcggcagccg
     7921 cgatctcggg ggaggacccg atccagacgg gcctgcaggg gtccgtgtac gcactgcgca
     7981 cggtcgtgct gcccttcatc tggatcttca atccccagct gctgctgctg gacctcgagg
     8041 gctggcagga gctcgtctac gtggtggtgg cctcgacggt ggcctgcctc acgttcgcgg
     8101 ccgcaaccat gggctggttc caggtgaaga gccgctggtg ggaaaccgcg atcctgctgc
     8161 tcgccacgct gatgctcttc cgccccaatt tcttcatgga ccagctctac gaaccatggg
     8221 aatcgcgccc ggcgacggcg ttgtaccaga tcgcgacgga gcttcccgag gattcgcgca
     8281 tcgtgctcgt catcgaggga acgaacgtgg agggcgatga cgtccgcaag accatttcgg
     8341 tgcagctgac caagcccgga gagggccgcg cgcgcttcgc agaagcgggc gtgaccttca
     8401 gcgtgctggg cgacgaaacg cggatcggga cggtcaagtt cggcagccgc gccaagcgcg
     8461 gcggcttcga gcagggctgg aaggtgaggg aaatcaaggt ccgctcggat gcgccgtcgg
     8521 aacactgggt gttcataccg ggcttcgcgc tcatcgccct ggtgttctac ctgcagcggc
     8581 ggcggatgcg cccggccggt gcagcacctg cgatggcctg acgactgata tcctcatccg
     8641 gaggacccac gtgcccacc
//
LOCUS       DASUEV010000088         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_941292, whole genome shotgun sequence.
ACCESSION   DASUEV010000088 DASUEV010000000
VERSION     DASUEV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941292"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFV55_02785"
     CDS             complement(<1..958)
                     /locus_tag="VFV55_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4943249.1"
                     /translation="MPSDSSAAGGRAPLPLALLLACTVLAHAAFNGSRLTLSLNALSL
                     DASPLAVGVMMSLFAALPMLLGVVSGRLVDRIGVRRPLIGGTVLLAACVALPGLVPAF
                     PVLYVAAAGIGTAFMVFHIAVQHAVGAGSSEAERKANFGWLALGFSISNFIGPTSAGF
                     MIDSIGHRATFLVLALSALGALSLVAWKRHAFVDNHGAAKAADTRNALQLLRDPELRR
                     VFLVTAMLASAWDLFVFVMPIYGTEIGLSASTIGLILGSFALATIVIRVALPWISRHV
                     RDWPMITATFVIACIAYALFPLVETVPLLAAIAFLLGLGLGST"
     gene            complement(969..1847)
                     /gene="htpX"
                     /locus_tag="VFV55_02790"
     CDS             complement(969..1847)
                     /gene="htpX"
                     /locus_tag="VFV55_02790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX4943250.1"
                     /translation="MKRIVLFLATNLAVMLVLSVTLNVLGVNRFLAAEGLNVGMLVVF
                     SAVVGFTGAFISLLMSKSMAKWSTGAQVIVTPQNADEQWLVGTVRRLSEKAGIGMPEV
                     AMYEGEPNAFATGAFRDSSLVAVSTGLLHAMTKEEVEAVLAHEVAHVANGDMVTLTLV
                     QGVVNTFVVFIARVVGYVVDKAVFRTERGVGAGYYITVVVCEIVFGILASIIVAWFSR
                     QREFRADAGAARFMGTPRPMVSALRRLGGLEPGALPQSLQAFGIAEGHNRFLALFASH
                     PPIPERIAALETARPA"
     gene            complement(1896..2366)
                     /gene="ssb"
                     /locus_tag="VFV55_02795"
     CDS             complement(1896..2366)
                     /gene="ssb"
                     /locus_tag="VFV55_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX4943251.1"
                     /translation="MASVNKVILIGNLGRDPETRYMPDGGAVTNVSIATTESWKDKNG
                     EKQEKTEWHRIAFFGKLAEIAGEYLKKGSQVYVEGRLQTRKWQDKDGQDKYTTEIIAD
                     RMQMLGSRQGMGGGDREREPGGEREGASRPAAKPAAGAKPAGSKFDDFEDDIPF"
     gene            complement(2382..3584)
                     /locus_tag="VFV55_02800"
     CDS             complement(2382..3584)
                     /locus_tag="VFV55_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4943252.1"
                     /translation="MSARELRASFSLASIFGLRLFGMFIILPVFALYAERLPGWNLTL
                     VGIALGAYGLTQALLQAPYGWVSDRVGRKPVMVAGLAIFAFGSLICALADAPWAVILG
                     RTVQGAGAISGVALAAAADLTRPSQRTKSMAIIGSTIGVSFALSFAVAPFLQKSIGVP
                     GIFALTGLLAVAAVAIVAWVVPPQPAKRHDHAHVPLGAVLRDPELVRLNVGIFGLHAI
                     LMALFIVVPLALVRAGLPAGSHSGIYLGAVGTGFLLMLPFVAVRTLARHEKAVFLGAV
                     VVLALGIGVLAVASDRLWPLAAGLVIFFTAFNVLEAKLPALVSKAAPPTAKGVASGIY
                     SSVQFLGTFVGGAAGGAVAQHFGPVAVLASCLAIAIAWLAVAWPMEEPAAREPEPHDS
                     HEPQATQP"
     gene            3724..>5279
                     /locus_tag="VFV55_02805"
     CDS             3724..>5279
                     /locus_tag="VFV55_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX4943253.1"
                     /translation="MDHIRIRGARTHNLKNVHLDIPRNRLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPHCPEHGLALSAQSVSQMVDHVLALPEDTRVMVLAPLVTGRKG
                     EQAELFEELRSQGFVRLRINGEVHEIDQLPKLDRNRKHTIEIVVDRLKVRADTKQRLA
                     ESFETALRHADGRAIAVEMQSGKEHLFSAKFACPLCSYSLPELEPRLFSFNNPMGACP
                     RCDGLGQVTFFDPKRVVAFPHLSLAGGAIRGWDRRNQFYFSMLVSLAQHYGFDVETPF
                     ESLPERAQTVVLDGSGDEKIAFRYPGERGKSAVREHSFEGILPNLERRFRETDSAAVK
                     EELAKYLNDRACPDCGGARLRREARHVRVAGRSIEEVSAFPLKDAQPFFEKLVLPGNR
                     QAIAEKILREIANRIRFLNDVGLDYLCLMRSADTLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLH"
BASE COUNT          912 a         1806 c         1822 g          739 t
ORIGIN      
        1 gggtggagcc cagacccagg ccgagcagga aggcgatcgc ggcgagcagc ggcaccgtct
       61 cgaccagggg gaagagcgcg taggcgatgc aggcgatcac gaaggtggcc gtgatcatcg
      121 gccagtcgcg cacgtggcgc gagatccacg gcagggccac acggatgacg atggtggcga
      181 gcgcgaagct gccgaggatg aggccgatgg tcgaggcgct cagcccgatc tccgtgccgt
      241 agatcggcat gacgaacacg aacaggtccc aggcgctggc gagcatcgcc gtcaccagga
      301 acacgcgccg cagctcgggg tcgcgcagca gctgcagggc attgcgcgtg tcggccgcct
      361 tcgccgcgcc gtggttgtcg acgaaggcgt ggcgcttcca ggcgacgagc gagagcgccc
      421 cgagggcgga cagcgcgagg acgaggaacg tcgcgcggtg gccgatcgag tcgatcatga
      481 agcccgcgga ggtcggcccg atgaagttgg agatcgagaa gcccagcgcc agccagccga
      541 agttggcctt gcgctcggcc tcgctgctgc cggcgccgac cgcgtgctgc accgcgatgt
      601 ggaagaccat gaaggccgtg ccgatgccgg cggcggccac gtacagcacc gggaacgccg
      661 gcaccagccc cggcagcgcc acgcacgccg ccagcagcac ggtgccgccg atgagcgggc
      721 ggcgcacgcc gatgcggtcg accaaccgcc ccgagaccac gcccagcagc atcggcagcg
      781 ccgcgaagag cgacatcatc acgcccaccg cgagcggcga ggcgtcgagc gacagcgcgt
      841 tcagcgagag cgtgaggcgg ctgccgttga acgcggcgtg cgcgagcacg gtgcaggcga
      901 gcagcagcgc gagcggcaac ggcgcgcggc cgcccgccgc gctggaatcg ctgggcaaag
      961 gccccgggct acgccgggcg cgccgtttcc agggcggcga tgcgctcggg aatcggcggg
     1021 tggctcgcga acagcgcgag gaagcggttg tgtccctcgg cgatgccgaa cgcctgcagc
     1081 gactgcggca gcgcgcccgg ctcgaggccg ccgagacggc gcagcgccga gaccatgggc
     1141 cgcggtgtgc ccatgaagcg ggccgcgccc gcatcggccc ggaattcgcg ctggcgcgag
     1201 aaccaggcga cgatgatcga ggcgaggatt ccgaacacga tctcgcagac gacgacggtg
     1261 atgtagtagc cggcgcccac gccgcgctcg gtcctgaaga cggccttgtc gaccacatag
     1321 ccgacgacgc gggcaatgaa tacgacgaag gtattcacca ccccctggac gagcgtgagc
     1381 gtgaccatgt cgccgttggc gacgtgcgcc acctcgtggg ccaggacggc ttccacttcc
     1441 tccttcgtca tcgcgtgcag gagccccgtc gagacggcga ccagcgacga gtcgcggaag
     1501 gcgcccgtgg cgaacgcgtt gggctcgccc tcgtacatcg cgacctccgg catgccgatg
     1561 ccggcctttt ccgacaggcg ccgcacggtg ccgacgagcc attgctcgtc cgcgttctgc
     1621 ggcgtgacga tcacctgcgc gcccgtggac cacttggcca tggacttcga catcagcagc
     1681 gagatgaacg cccccgtgaa gcccaccacc gcggagaaca ccacgagcat cccgacattc
     1741 aatccctcgg cggcgaggaa ccggttcacg ccgagcacgt tgagcgtgac cgaaagcacc
     1801 agcatcaccg cgaggttcgt ggcgaggaag agaacgatgc gtttcatggc ggcgttccgt
     1861 gcaagttcgg tggaaaggtc attctaccgg cacccctaga acggaatgtc gtcctcgaaa
     1921 tcgtcgaact tggagccggc gggcttcgcc ccggccgcgg gctttgcggc gggacgggat
     1981 gcaccctcgc gctcgccgcc gggttcgcgt tcccggtcgc cgccgcccat gccctggcgc
     2041 gaaccgagca tctgcatgcg gtcggcgatg atctcggtgg tgtacttgtc ctgcccgtcc
     2101 ttgtcctgcc acttgcgcgt ctgcagccgc ccctcgacgt acacctggct gcccttcttg
     2161 aggtattcgc cggcgatctc cgcgagcttg ccgaagaagg cgatgcggtg ccattccgtc
     2221 ttctcctgct tttcgccgtt cttgtccttc caggactcgg tggtcgcgat cgacacgttg
     2281 gtgacggcac cgccgtcggg catgtagcgc gtctcgggat cgcggccgag gttgccgatg
     2341 aggatgactt tgttgacgga tgccatggtt ttctcccggg gtcagggttg ggttgcttgc
     2401 ggctcgtggg aatcatgagg ctccggctcc cgtgcggccg gttcctccat cggccaggcg
     2461 acggcaagcc aggcgatcgc gatggcgagg caggacgcga ggacggccac ggggccgaaa
     2521 tgctgcgcga cggcgccgcc cgccgctccg ccgacgaagg tgccgaggaa ctgcacgctc
     2581 gaatagatgc cggacgcgac tcccttggcc gtgggcggcg ccgccttcga cacgagcgcg
     2641 ggcagcttcg cctctagaac gttgaaggcg gtgaaaaaga tgacgagccc tgccgcgagc
     2701 ggccacaacc ggtcggaggc cacggccagc acgccgatgc ccagggccag gaccacgacg
     2761 gcgccaagga agacggcctt ctcgtggcgt gccagggtcc gcaccgcgac gaagggaagc
     2821 atgagcagga atccggtgcc gaccgcaccc aggtagattc ccgagtgcga tcccgccggc
     2881 aggccggcgc gcacgagcgc gagcggcacc acgatgaaaa gcgccatgag gatcgcgtgc
     2941 aggccgaaga ttccgacgtt gagccgcacc agctccgggt cgcggagcac ggcaccgagc
     3001 ggcacgtgcg cgtggtcgtg ccgctttgcc ggctgcggcg gaaccaccca cgccacgatc
     3061 gccacggcgg ccaccgcgag cagcccggtc agcgcgaaga tgccggggac gccgatcgac
     3121 ttctgcagga acggcgccac ggcgaacgac agggcgaacg agacgccgat cgtcgagccg
     3181 atgatcgcca tcgacttggt gcgttggctg ggccgcgtga ggtccgccgc cgccgcgagc
     3241 gccacgccgg agatcgcccc cgcgccctgc acggtccggc cgaggatcac ggcccagggc
     3301 gcatcggcga gcgcgcagat gaggctgccg aacgcgaaga tcgcgagccc cgccaccatc
     3361 accggcttgc gcccgacgcg gtccgatacc cacccgtacg gagcctgcag gagggcctgc
     3421 gtgagcccgt aggcgccgag ggcgatgccc accagggtga ggttccagcc cggcagccgt
     3481 tcggcgtaca gggcgaacac gggcaggatg atgaacatcc cgaaaagtcg cagcccgaag
     3541 atcgaggcga gcgaaaagct cgcccggagc tcccgggcgc tcatgccgac cggagaaggg
     3601 gccaatgggg ggattcgaac gggatggaac gctcaggtat attagcagat tgtccttccg
     3661 gcccggcttg cgcgccgggg gccccgaaga ccagaacagg accacgatac atcccgaccg
     3721 ggcatggacc acatccgaat ccgcggcgcc cgcacgcaca acctcaagaa cgtccacctc
     3781 gacattccgc gcaaccggct ggtggtgatc acgggcctgt ccggctcggg caagtcgtca
     3841 ctcgcgttcg acaccctcta cgcggagggg cagcggcgct acgtggagtc cctgtcggcc
     3901 tatgcccggc agttcctgca gctcatggaa aagccggacg tggacctcat cgaggggctg
     3961 tcgccggcca tctcgatcga gcagaaggcg acctcgcaca acccccgctc gacggtcggg
     4021 acggtaaccg agatccacga ctacctgcgc ctgctcttcg cgcgcgtcgg cgaaccgcat
     4081 tgcccggaac acggcctggc gctttccgcg caaagcgtct cgcagatggt cgaccacgtt
     4141 ctcgcgttgc ccgaggacac gcgcgtgatg gtgctggccc ctctcgtgac gggccgcaag
     4201 ggcgagcagg ccgaactctt cgaggagctt cgctcgcagg gcttcgtgcg gctgcgcatc
     4261 aatggcgaag tccacgagat cgaccagttg ccgaaactcg accgcaaccg caagcacacc
     4321 atcgagatcg tggtcgaccg cctgaaggtc cgggccgata ccaagcagcg gctcgcggaa
     4381 tccttcgaga cggcgctgcg ccatgccgac gggcgcgcca tcgcggtgga aatgcagtcc
     4441 ggcaaggagc acctcttctc ggcgaagttc gcctgcccgc tgtgcagcta ctcgctcccg
     4501 gagctcgagc cgcgactgtt ctcgttcaac aatcccatgg gcgcgtgccc gcggtgcgac
     4561 gggctcgggc aggtcacgtt cttcgacccc aagcgcgtcg tcgcgtttcc gcacctttcg
     4621 ctcgcgggcg gtgccatccg cggctgggac cggcgcaacc agttctattt ttcgatgctc
     4681 gtgtcgctcg cgcagcacta cggcttcgac gtggagacgc cgttcgagag cctgccggag
     4741 cgcgcccaga ctgtggtcct ggacggatcc ggcgacgaga agatcgcgtt ccgctacccc
     4801 ggcgagcgcg gcaaatcggc cgtgcgcgag cattccttcg aggggatcct cccgaacctg
     4861 gagcgccgct tccgcgagac cgattcggcg gcggtaaagg aggagcttgc caagtacctg
     4921 aacgaccgcg catgccccga ctgcggcggc gcaaggctgc gccgggaggc gcggcacgtc
     4981 cgcgtggcgg gccgctccat cgaggaagtt tcggcgtttc ccctgaagga cgcgcaaccc
     5041 ttcttcgaga agctggtcct ccccggcaac cgccaggcga tcgcggagaa gatcctgcgc
     5101 gagatcgcca accgcatccg gttcctcaac gacgtgggcc tggactacct ctgcctcatg
     5161 cgctcggccg acaccctctc cggcggcgag gcgcagcgca tccgccttgc gagccagatc
     5221 ggctccgggc tcaccggcgt gatgtacgtg ctggacgaac cttccatcgg gctgcacca
//
LOCUS       DASUEV010000089         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1514364, whole genome shotgun sequence.
ACCESSION   DASUEV010000089 DASUEV010000000
VERSION     DASUEV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514364"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFV55_02810"
     CDS             <1..945
                     /locus_tag="VFV55_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4943254.1"
                     /translation="AAAARSYVLPEADVVMLVGARLNWLLSHGKGKTWGGKAPKEWGG
                     QKFIQVDISPQEADSNVRIDAPVVGDIGSCVSALLAGIDSSWPKPPADWLGAIAERKN
                     RNIAKMGETLARNPAPMNFHSALNVVRDIVKANPDAMLVNEGANALDFTRSIVDMYKP
                     RKRLDVGTWGVMGIGMGFAVAAAVVSGEQVIAIEGDSAFGFSGMEVETICRYNLPVCV
                     VILNNNGVYRGDEVNPTGGRDPSPLVFVKDARYEKLMEAFGGVGVHATTPAELRKAME
                     EAIRSRRPTLINAVIDETAGTESGRITALNPTAKKKQP"
     gene            998..2269
                     /gene="frc"
                     /locus_tag="VFV55_02815"
     CDS             998..2269
                     /gene="frc"
                     /locus_tag="VFV55_02815"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR03253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX4943255.1"
                     /translation="MEALKGVRILDMTHVQAGPTCSQLLAWMGADVIKFEPPQGDATR
                     GQLRDIPGADSLYFTMLNCNKRSITVNMKSAEGKLVFVDLLKKCDIMMENFGPGVLER
                     LGFTWEKIHELNPKIVVGSIKGFGSSGPYAEFKAYENVAQAMGGSMSTTGVPNGRPFV
                     TGAQIGDSGTGLHLAIGLLAALHQAQRTGQGQYVEVAMMDGIMNLCRVKWRDHQRLTR
                     GRLPEYSVPTHQNMGAVPRAGNDSGGGQLGNAIRCAPGGTNDFIYVVVQEAVWEALAA
                     RVGPEVGLPDFAADPRFATIDERRRHQNELWEILGEFALKYDKRHMMKILNELDVPCG
                     PIMSTEDLANDEHVRGREMYVELDHPQRGKWFNVGMPIKMSASPAKIERSPLLGEHTE
                     EVLKTVLGYDDGKIAAMKQAGAFSAPPRKVA"
     gene            2461..>2662
                     /locus_tag="VFV55_02820"
     CDS             2461..>2662
                     /locus_tag="VFV55_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943256.1"
                     /translation="MRIAIIGQQDFGKAVLEAFLARGDTVAGVFVAPEKEGAKPDPMK
                     VAAQEKGLKVFQFPSLKSGDATK"
BASE COUNT          515 a          831 c          917 g          399 t
ORIGIN      
        1 gctgccgctg cccgctccta tgtgctgccc gaggccgacg tggtgatgct ggtcggcgcg
       61 cgactgaact ggctgctctc gcatggcaag ggcaagacct ggggcggcaa ggccccgaag
      121 gagtggggcg gccagaagtt catccaggtc gacatctcgc cgcaggaggc ggacagcaac
      181 gttcgcatcg acgccccggt ggtcggcgac atcggttcct gcgtgtcggc gctgctggcc
      241 ggcatcgact ccagctggcc gaagccaccg gcggactggc tgggcgccat cgccgagcga
      301 aagaacagga acatcgcgaa gatgggcgaa accctggcca ggaatccggc gccgatgaat
      361 ttccacagcg cgctgaacgt ggtgcgcgac atcgtcaagg cgaacccgga cgccatgctg
      421 gtgaacgagg gcgccaacgc gctggacttc acccgcagca tcgtcgacat gtacaagccg
      481 cgcaagcgcc tggacgtcgg aacctggggc gtgatgggca tcggcatggg cttcgccgtg
      541 gcggcggccg tggtgagcgg cgagcaggtg attgcgatcg agggcgacag cgccttcggc
      601 ttctccggca tggaagtcga gaccatctgc cgctacaacc tgccggtctg cgtggtgatc
      661 ctgaacaaca acggcgtcta ccgcggcgac gaagtcaatc cgaccggcgg ccgcgatcct
      721 tcacccctgg tgttcgtcaa ggatgcgcgc tacgaaaagc tgatggaggc cttcggcggc
      781 gtgggcgtgc acgccacgac cccggccgag ttgcgcaagg cgatggaaga agccatccgc
      841 tcgcgcaggc ccacgctcat caatgccgtc atcgacgaga ccgccggcac cgaaagcggt
      901 cgcatcacgg cgctgaaccc gaccgcgaag aagaaacagc cctgacgcag gtataagtaa
      961 aatgcttgaa accacggatg aataggaagc gacgcgcatg gaagctctca aaggcgtaag
     1021 aattctcgac atgacccacg tgcaggcggg cccgacgtgc tcgcagttgc tcgcttggat
     1081 gggcgcggac gtgatcaagt tcgagccgcc gcagggcgac gcgacgcgcg gccagttgcg
     1141 cgacatcccc ggcgccgaca gcctctactt cacgatgctc aactgcaaca agcgctcgat
     1201 cacggtcaac atgaagagcg ccgagggcaa gctcgtcttc gtcgacctgc tgaagaagtg
     1261 cgacatcatg atggagaact tcggccccgg cgtcctcgag cgcctcggct tcacctggga
     1321 aaagatccac gagctcaacc cgaagatcgt cgtgggatcc atcaagggtt tcggctcgtc
     1381 cgggccgtac gcggagttca aggcgtacga gaatgtcgcg caggcgatgg gcggctcgat
     1441 gagcaccacg ggcgtgccca acggccgccc gttcgtgacc ggcgcgcaga tcggcgactc
     1501 gggcacggga ctgcacctcg cgatcggcct gctggccgcg ctgcaccagg cgcagcgcac
     1561 cggccagggc cagtacgtcg aggtcgcgat gatggacggc atcatgaacc tgtgccgcgt
     1621 caagtggcgt gaccaccagc ggctcacgcg cgggcggctg cccgagtact cggtgcccac
     1681 gcaccagaac atgggcgcag tgccgcgggc gggcaacgac tccggcggcg gccagctcgg
     1741 caacgccatc cgctgcgccc cgggcggcac caacgacttc atctatgtcg tcgtgcagga
     1801 agcggtgtgg gaagctctcg ccgcgcgcgt cgggccggag gtgggcctgc cggacttcgc
     1861 ggccgatccg cggttcgcga cgatcgacga gcgccgccgg caccagaacg agctgtggga
     1921 gatcctgggc gagttcgcgc tcaagtacga caagcgccac atgatgaaga tcctgaacga
     1981 gctcgacgtg ccctgcggcc ccatcatgtc gacggaggac ctcgccaacg acgagcacgt
     2041 gcgcgggcgc gagatgtacg tggagctcga ccatccgcag cgcggcaagt ggttcaacgt
     2101 cggcatgccg atcaagatgt cggcctcgcc ggcgaagatc gagaggagcc cgctgctggg
     2161 cgagcacacg gaagaggtcc tcaagacggt gctcggctac gacgacggca agatcgccgc
     2221 gatgaagcag gcgggggcgt tttccgcgcc gccgaggaag gtggcgtagg ggagtggtgc
     2281 gggccaaagg cgtcgggcac ggatgaacac ggatgtctcg tgatcgaaat ggcgcagtgc
     2341 caggagggac gccgtcgtcc tttccggcat cgcggcttcc gctcaggatt catccgcgtt
     2401 catccgcagt tatccgtatt catccgtgtt ccagaggttt actgacggga taattcgaaa
     2461 atgcgcattg cgatcatcgg gcagcaggat tttggcaagg cggtccttga agctttcctc
     2521 gcgcgcggcg acacggtcgc gggcgtgttc gtggcgccgg agaaggaggg cgccaagccc
     2581 gaccccatga aggtagccgc gcaggaaaag gggctcaagg tgttccagtt cccctcgctc
     2641 aagagcggtg acgcgacgaa gg
//
LOCUS       DASUEV010000090         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_356547, whole genome shotgun sequence.
ACCESSION   DASUEV010000090 DASUEV010000000
VERSION     DASUEV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356547"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFV55_02825"
     CDS             <1..212
                     /locus_tag="VFV55_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX4943257.1"
                     /translation="TTGRLTSEMVIKAAQMGIPFLVSRSGLTKMGHEIATTVGITMIG
                     RAINRRYLIFTGGERFVRRASPAAA"
     gene            224..631
                     /locus_tag="VFV55_02830"
     CDS             224..631
                     /locus_tag="VFV55_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX4943258.1"
                     /translation="MASTAPETAVARCHCGGVEVVAAFPSRFCAHCYCWSCRTTHAAG
                     VVTWIGFKRAQVRFAKGEELLRKYESSRGTVRRFCNTCGTRITFESAHGRWADEVHLP
                     LALFVTPVDREPAGNSFPSERPAWAPFHAFPDD"
     gene            624..1205
                     /gene="mobA"
                     /locus_tag="VFV55_02835"
     CDS             624..1205
                     /gene="mobA"
                     /locus_tag="VFV55_02835"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX4943259.1"
                     /translation="MIEVTGLVLAGGQGSRMGGVDKGLAPFRGRALVEHVIERLSPQV
                     DEILVNANRNPEAYAGFGHRVIADEIPGFAGPLAGFERGLAHASGKLVATVPCDSPFL
                     PADLVSRLRAALEAANADLAVARTGEQPHPVFCLMRRGVQQSLHEFLSTGQRKIDKWY
                     TALRVVEVAFDDEPEAFANINTRDELAGLEKPP"
     gene            1202..2470
                     /gene="glp"
                     /locus_tag="VFV55_02840"
     CDS             1202..2470
                     /gene="glp"
                     /locus_tag="VFV55_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX4943260.1"
                     /translation="MTKLTITQASCADDYDPDSMPVDSARAFIHRFLDPVSATVRVPI
                     RSALGRVLAEDVVSPVDVPSHRNSAMDGWAMRGSDLQPGAETTLDEIGSSFAGRPFAG
                     RVGPGQCVRIMTGGVVPEGADTVVMQERAQADGKRVTFAAGQKTGQNLRQAGEDLRRG
                     DVALAKGRIVRPADAGLLASLGIGEVGVYRPLRVAFFSTGDELKSIGTALGEGEIYDS
                     NRYTIHGMLTRLGCEVLDMGVVRDDPALLERAFREAAANADVVITSGGVSVGEADFVK
                     GLLGKLGEVVFWKIAMKPGRPLAYGKIGGAHFFGLPGNPVSVMVTFYQFVREALLKLM
                     GVDPVAAIPTFPATCTAKLRKAPGRTEFQRGILSRGTDGAWTVRPTGDQGSGILKSMS
                     EANCFIILGDAVGNVEAGTRVEVQLLEGIV"
     gene            2467..2946
                     /locus_tag="VFV55_02845"
     CDS             2467..2946
                     /locus_tag="VFV55_02845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4943261.1"
                     /translation="MSKAPPFVPATEHEGTGLDYLRRMVAGEIASVPIGDTLGFRAID
                     VEHGRIVIAGTPDRRSYNLIGTVHGGWAASILDSAMALATLSSLDEHHLFTTLDIKIN
                     YVRGMTAETGEVRAEGRVLNAGRRVILSESKLTDSAGKLLATGQSTCLVIPRKAKAS"
BASE COUNT          501 a         1111 c         1080 g          481 t
ORIGIN      
        1 acaccaccgg ccggctcacc tcggagatgg tcattaaggc ggcgcagatg ggcattccct
       61 tcctcgtctc gcgctccggc ctcaccaaga tgggccacga gatcgcgacg accgtcggca
      121 tcacgatgat cgggcgcgcg atcaatcgcc gctacctgat cttcacggga ggcgagcggt
      181 tcgtccggcg cgcgtccccg gcggccgcct gaggccgcac atggtggcga gcaccgctcc
      241 cgaaacggcc gtcgcgcgct gccattgcgg cggcgtcgag gtcgtggctg cctttccctc
      301 gcgtttctgc gctcactgct actgctggag ctgccgcacc acccacgcgg caggcgtcgt
      361 cacctggatc ggcttcaagc gggcgcaagt ccgcttcgcg aagggcgagg aactgctgcg
      421 caagtacgaa tcgtcgcgcg gcactgttcg ccggttctgc aacacgtgcg gcacccgtat
      481 caccttcgaa tccgcgcacg gccgatgggc ggacgaagtg caccttccgc tggcactctt
      541 cgtcacgccg gtcgaccgcg agcccgccgg gaattccttc ccgtcggagc gccccgcctg
      601 ggcccccttc cacgccttcc ccgatgattg aagtgaccgg actcgtgctc gccggcggcc
      661 agggaagccg catgggcggc gtcgacaagg gcctcgcgcc gttccgcggg cgcgcgctcg
      721 tggagcatgt catcgagcgc ctctcgccgc aggtcgacga gatcctggtg aacgccaacc
      781 gcaatcccga ggcctatgcg ggattcgggc accgcgtcat cgcggacgaa ataccgggat
      841 tcgcaggacc gctggccggt ttcgagcgcg ggctggcgca cgcgagcggc aagctggtgg
      901 ccacggtgcc gtgcgactcg cccttcctgc ccgcggacct cgtctcccgc ctgcgcgcgg
      961 ccctggaagc cgccaacgcc gatctcgcgg tggcccggac gggagagcag ccccaccccg
     1021 tcttctgcct catgcgccgc ggcgtgcaac agtcgctgca cgaattcctg tccacgggcc
     1081 agcgcaagat cgacaagtgg tacaccgcgc tgcgcgtggt ggaggtcgcg ttcgacgacg
     1141 agcccgaagc cttcgccaac atcaacaccc gcgacgaact cgcgggactc gagaaaccgc
     1201 catgaccaag ctcaccatca cgcaggcgtc ctgcgcggac gattacgatc cggattcgat
     1261 gccggtcgac agcgcccgcg ccttcatcca ccgtttcctc gaccccgtct ccgcgacggt
     1321 tcgcgttccc atccgcagcg cgctgggccg cgtgctcgcc gaggacgtcg tttccccggt
     1381 ggacgtgcca tcgcaccgca actccgcgat ggacggctgg gcgatgcgcg gctcggacct
     1441 gcagcccggc gccgaaacga cgctcgacga gatcgggtct tcgttcgccg ggcggccgtt
     1501 cgcgggccgc gtcgggccgg ggcagtgcgt gcggatcatg accggcggcg tggtgcccga
     1561 aggcgccgac acggtcgtca tgcaggagcg cgcgcaggcc gacggcaagc gcgtcacctt
     1621 cgccgccggg cagaagacgg ggcagaacct gcgccaggcc ggcgaggacc tcaggcgcgg
     1681 cgacgtggcg ctggcgaagg gacgcatcgt gcgccccgcg gacgcagggc ttctcgcctc
     1741 gcttggcatc ggcgaggtcg gcgtctaccg gccgcttcgc gtggctttct tttccaccgg
     1801 cgacgagttg aagtcgatcg ggaccgccct gggcgagggc gagatctacg actccaaccg
     1861 ctacacgatc cacggcatgc tcacgcgcct gggctgcgaa gtgctcgaca tgggcgtcgt
     1921 gcgcgacgac ccggcgctcc tcgaaagggc gttccgcgag gcggcagcca acgccgacgt
     1981 cgtgatcacg agcggcggcg tgtcggtggg cgaggccgac ttcgtgaagg gcctgctcgg
     2041 caagctgggc gaagtggtgt tctggaagat cgccatgaag ccgggccggc cgctcgccta
     2101 cggcaagatc ggcggcgcgc acttcttcgg cctgcccggc aacccggtgt cggtgatggt
     2161 caccttctac cagttcgtgc gcgaggcgct gctcaagctc atgggcgtgg atcccgttgc
     2221 cgcaatcccg accttcccgg ccacatgcac ggcgaagctc aggaaggcgc ccggacgcac
     2281 cgaattccag cgcggcatcc tgtcgcgcgg gaccgacggc gcctggaccg tgcggccgac
     2341 cggcgaccag gggtcgggaa tcctcaagtc gatgagcgag gccaactgct tcatcatcct
     2401 cggcgacgcg gtgggcaatg tcgaggccgg cacccgggtg gaggtccagc tcctcgaggg
     2461 catcgtgtga gcaaggctcc acccttcgtc ccggccaccg agcacgaggg aacgggcctc
     2521 gattacctgc ggcgcatggt cgcgggcgag atcgcctcgg tgcccatcgg cgatacgctg
     2581 ggtttccggg ccattgacgt cgagcacggc cgcatcgtca tcgcgggaac gcccgaccgg
     2641 cgctcgtaca acctcatcgg cacggtccac ggcggctggg cggcgtccat cctcgacagc
     2701 gccatggcgc tggccaccct ttcctcgctc gacgagcacc acctcttcac caccctcgac
     2761 atcaagatca attacgtgcg cggcatgacc gccgagacag gcgaagttcg cgccgaaggc
     2821 agggtgctga acgccgggcg gcgcgtgatc ctgtcggagt cgaagctcac cgacagcgcc
     2881 ggcaagctcc tcgcgaccgg gcagagcacc tgcctcgtga ttccccgcaa ggccaaggcc
     2941 tcatagtcgg cagcggctga ggtcgagggc aatcccggtt gaccagggcg aggcggtagc
     3001 ggtggaagtc gatcgcaatc gtgggcacgc gggtcgagga ggcggtggcg gtggcggtgg
     3061 aagtcgatcg caatcctggg cacgcaatcg tgaccgtcgg atgggtgccc ggaaagtcga
     3121 ggccaggcag aacaagccag gcctcgactt tccacccatc taatgacgag ggc
//
LOCUS       DASUEV010000091        10750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_356654, whole genome shotgun sequence.
ACCESSION   DASUEV010000091 DASUEV010000000
VERSION     DASUEV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356654"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /gene="hslU"
                     /locus_tag="VFV55_02850"
     CDS             complement(<1..176)
                     /gene="hslU"
                     /locus_tag="VFV55_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230746.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEX4943262.1"
                     /translation="MTPQEIVHELDKHIVGQGAAKKAVAIALRNRWRRSQVAEPLRSE
                     ITPKNILMIGPTGVG"
     gene            complement(233..772)
                     /gene="hslV"
                     /locus_tag="VFV55_02855"
     CDS             complement(233..772)
                     /gene="hslV"
                     /locus_tag="VFV55_02855"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX4943263.1"
                     /translation="MTGETFHGTTIICVRRGAQVAMGGDGQVTLGNVVIKASARKVRR
                     LYSDRILAGFAGATADAFTLFERFEAKLEKHQGNLLRSAVELAKDWRTDRMLRRLEAM
                     LAVADRESSLLITGNGDVLEPEHGIVAMGSGGSYAQAAAIALLGHTELSPADIVKRSL
                     EIAGEICIYTNQIHVVETL"
     gene            complement(780..1880)
                     /locus_tag="VFV55_02860"
     CDS             complement(780..1880)
                     /locus_tag="VFV55_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4943264.1"
                     /translation="MASPSSGVPSTGNLKSAPRQPARVGNEDYHSTLLPHAQEAAVLY
                     AANLAAPAEAILKGETRNPSSRSNKQPWLMLFDLYEATQNRRAYDELSLVYTVKFEQS
                     PPAWAGGAEAATDPRRAQNRERNDLFVLRPNAAGELAQEIEKFVAFAESMGTVRLDTG
                     KLATITAAESALLAAALKRLRRAGMPMWFNNTDSLEQLLRAAFNERTSDTTRGYWMLL
                     FELFVVQGKMEPFEELGLEYAVAFEISPPNWEVYVNPVAAATARTQAVTAAPSAPEAG
                     FVLKGVVSTASQGEFAELAARAASGTELVVDMGKVLRIDFAATAQFFEVIKAIQLAGK
                     RVILSNLSELNAALLEAFGFNRHAILIRRKAN"
     gene            complement(2169..3164)
                     /locus_tag="VFV55_02865"
     CDS             complement(2169..3164)
                     /locus_tag="VFV55_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4943265.1"
                     /translation="MAAVAQPDKDLIPVTILTGFLGAGKTTLLNRILTEQHGERIAVI
                     ENEFGETGVDTEILVEDREEQIVEMNNGCLCCTVRGDLVRILGDLGRKRRQGKLKFDR
                     VVIETTGLANPGPVAQTFFMDEDIHDEYLLDAVITVVDAKHGQKQLDEHDEARAQVGF
                     ADRILLSKTDLVGEAETADLMDRLRDMNPRAHQKKVHFGETDIREILDIRGFNLNAAL
                     EIDPGFLAEGHHHHDDDVKSFVWRDPRPLQMEKIETFLSLMVQNYGADLLRYKGVLNI
                     QGEPRRMIFQGVHMLMGGTPGKPWAPGETRESVMVFIGRKIPRRLFEEGLAYCAA"
     gene            complement(3168..4229)
                     /locus_tag="VFV55_02870"
     CDS             complement(3168..4229)
                     /locus_tag="VFV55_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4943266.1"
                     /translation="MTATTVTPVTLLTGFLGSGKTTLLNRLLGHPEFGDCAVIVNEFG
                     PVAIDHVLVRTASENVVLLPSGCVCCQVAGDLVQALRDLYFKRAAGEIPAFRRAIIET
                     TGLADPAPLARTLIELPLVAARYSLAGIVTTVDGQHGSGQLDRHPEATKQAAVADRLV
                     ITKTDLAEPPALEALEGRLAALNPGAVRLRSARGDADPQRLLDTGLYRGSVRLADAGS
                     WLNAGAYRRVGDPMPAAHDPRVTAFAWFAGTPVSWPAFEDALATLLDLAGERILRLKG
                     LVNVAGEAGPVAVHAVQHTLYPPAPLPSWPDGDRRTRLVFIVRDLEESFVAQMLDSEL
                     GTLPYSALQQEGSVPCPER"
     gene            complement(4258..4698)
                     /locus_tag="VFV55_02875"
     CDS             complement(4258..4698)
                     /locus_tag="VFV55_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX4943267.1"
                     /translation="MGKSRTHYAAHLPAAQVRIRAAGERLTAPRAAVLAALLGSEAAL
                     THHELEGALRADLAVDRVTVYRVLDWLVAVGLAHRIPGEDRTWRFHANRDRSHGPHAH
                     FTCSRCGKTVCLEDVAVPPRVRLPRGFLPQRVELTVQGLCAACH"
     gene            complement(4751..5641)
                     /gene="xerC"
                     /locus_tag="VFV55_02880"
     CDS             complement(4751..5641)
                     /gene="xerC"
                     /locus_tag="VFV55_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX4943268.1"
                     /translation="MSPPTSALARYLDFLRHERRLAAHTLTAYSRDGALLGTLAEGRE
                     PGELRAADVRRFVAVLHGQGLSPRSLARLLSSWRGFFDWLVRHREIAVNPCKGIRAPR
                     AARTLPEALSPDDASRLVAFDDPSDAGIRDRALFELAYSCGLRVSELTGLDQGAVDAG
                     AGEARVTGKGSKTRIVPVGQPALEALSRWLPVRDRLAAPGEKALFIGRSGKRMSTREV
                     QRRIKRRAAAAGLSMDVHPHMLRHSFASHVLQSSGDLRAVQEMLGHASIASTQVYTHL
                     DFQHLAKVYDAAHPRARRKG"
     gene            complement(5625..6302)
                     /locus_tag="VFV55_02885"
     CDS             complement(5625..6302)
                     /locus_tag="VFV55_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX4943269.1"
                     /translation="MLTPEQVAEFLRRNPGFFEQNVDLLINIQIPHPHGGRAVSIGER
                     QLVAAREKTRLLEDKLRELIGFGEENDAVSEKVHRMACRLMLATGLDAVLDTTYLDLL
                     DHFAVPHVAVRLWNVAERDPETKEFADVAAEMHEFVAQMTTPYCGNHAVYESQGWFGE
                     AAPHLKSFALVALRHGEHCFGVLALASEEAQRFYPEMGTLYLTRIGELLSSALWRHLT
                     PVREPAD"
     gene            complement(6296..7180)
                     /gene="dapF"
                     /locus_tag="VFV55_02890"
     CDS             complement(6296..7180)
                     /gene="dapF"
                     /locus_tag="VFV55_02890"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX4943270.1"
                     /translation="MRLRFTKMHGQGNDFVVIDGVRQAVALTAAQVRAIADRHFGVGC
                     DQLLLVEPAREPGNDFRYRIWNADGGEVEQCGNGARCFARFVRDEGLTDKTELRVETA
                     RGVIRPRIEATGQVSVDMGPPRFAPAEVPFTAPAERPTYAIAVGGITIEASVLSMGNP
                     HAVQVVADVDAAPVTTQGPLIENHPAFPNRVNAGYLQVVARDHIRLRVWERGAGETLA
                     CGTGACAAVVAGIRRGLLDRRVRVTTRGGDLAIRWDGTAADMTPPFAPVIMTGNAVRV
                     FEGEIELPATGSLETTSC"
     gene            complement(7186..8073)
                     /locus_tag="VFV55_02895"
     CDS             complement(7186..8073)
                     /locus_tag="VFV55_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX4943271.1"
                     /translation="MVILRRLGSWLGWALLWLMHWLPTPVLGAIGRALGRLLYRFGRG
                     RVTDTNLALCFPGMPEAERKALGRRNFEALGRNAIEMSVMWFASDRRVLDLVAIRGRE
                     HLDRLRGTPVILLVPHFIGLNMGGARIANDFPGSASIYSRQKNPVLDRLFLHGRMRFG
                     EPILLSRQDGMRAIVKTLREGRLLYFLPDMDFGPRDAIFVPFFGVPAATVTALPRLAK
                     LGRAKVVPAIARQLERGYEIVVYPPWEDYPTGDIEADVRRMNEFIEERVREIPEQYFW
                     PHKRFKTRPPGEPGPYRRK"
     gene            complement(8067..8954)
                     /locus_tag="VFV55_02900"
     CDS             complement(8067..8954)
                     /locus_tag="VFV55_02900"
                     /inference="COORDINATES: protein motif:HMM:NF006487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX4943272.1"
                     /translation="MKLLLRFLAWLDLSANHRLGAFAGRLVFLLSPRYRLRTLENLRT
                     AGIAPDEAGTARLARDNAAEIGKGAAELAWALFRPVKELAGLVKELNGWDAVERLRAL
                     GRPLIFITPHLGGYDIAGRYLWSRLPILVMYRPHKLGWLDDVIREGRNRGAAPDGSNV
                     APATMAGVRMLLRHLRKGGCTVILPDQVPGEGEGEWVDFFGRPAYTMTLTGRLQEASG
                     AAVVFCFAERLPEGRGFRLHLRAREEPLPADRAAAALAVNQGIEDLIRIAPAQYLWGY
                     NRYKRPAGAPPAPGGALQW"
     gene            9036..10235
                     /gene="metK"
                     /locus_tag="VFV55_02905"
     CDS             9036..10235
                     /gene="metK"
                     /locus_tag="VFV55_02905"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX4943273.1"
                     /translation="MKSNYLFTSESVSEGHPDKVADQISDAVLDAILAQDPRSRVAAE
                     TLCNTGLVVMAGEITTHANVDYIQVARDTIKRIGYDNTDFGIDYKGCAVMVCYDKQSP
                     DIAAAVDHASDEYLNQGAGDQGMMFGYACDETPVLMPAPIYYAHRIVERQSQVRKDGR
                     LAWLRPDAKSQVTMRYENGKPVSIDTIVLSTQHAPDVSQEQIKEAVIELVIKPALPKE
                     MLKDTRYLVNPSGRFVVGGPQGDCGLTGRKIIVDTYGGACPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNVVAAGLARQCQIQLSYAIGVAKPINVTVYTEGTGAIADEKIAKLVEE
                     HFDLRPKGIVTMLDLLRPIYLKTAAYGHFGREEPEFMWETTDKAKLLRDAAGLKGAHA
                     KATATAD"
     gene            complement(10305..>10750)
                     /locus_tag="VFV55_02910"
     CDS             complement(10305..>10750)
                     /locus_tag="VFV55_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943274.1"
                     /translation="SAACLAAGCAAGPVHDTARHEAGAPDSRLAVDFPEPLRSHTLAN
                     MRDHLAALQEIQQALAESAFDRAADVSEKRLGMTSLTAHGAHDLAKFMPVGMQAAGTE
                     MHRSASRFSVEAANAGATGNVKPALAALARLTQQCVACHSGYRMK"
BASE COUNT         1692 a         3559 c         3866 g         1633 t
ORIGIN      
        1 cccacgcccg tgggcccgat catcaggatg ttcttcggcg tgatctcgct gcgcagcggc
       61 tcggccacct gcgagcggcg ccagcggttg cgcagggcga tcgcaacggc cttcttggcc
      121 gcgccctggc cgacgatgtg cttgtcgagc tcgtggacga tttcctgcgg cgtcatcatc
      181 gactccgccg gcgggacggg catttcggct ggggcgctca aggtcccgcc ggtcagaggg
      241 tctcgaccac gtggatctgg ttggtgtaga tgcagatctc gcccgcgatc tcgagggacc
      301 tcttcacgat gtcggccggg gagagctcgg tgtgcccgag cagggcgatc gccgcggcct
      361 gcgcgtagga gccgccgctg cccatggcga cgatgccgtg ctcgggctca agcacgtcgc
      421 cgttgccggt gatgaggaga gaggactcgc ggtcggccac ggcgagcatc gcctccagcc
      481 ggcgcagcat ccggtcggtg cgccagtcct tggcgagctc gaccgccgag cgcaggagat
      541 tgccctggtg cttctcgagc ttggcctcga agcgctcgaa cagcgtgaac gcgtcggccg
      601 ttgcgccggc gaaaccggcg aggatccggt cactgtagag acggcgcacc ttgcgcgcgg
      661 aagccttgat gacgacattg ccgagcgtca cctggccgtc gccgcccatg gcgacctgcg
      721 cgccgcggcg aacgcagatg atcgtggtgc cgtggaaggt ttcgccggtc atgacgggcc
      781 tagttcgcct tgcggcggat gaggatcgcg tggcgattga agccgaacgc ctcgagcaac
      841 gccgcattca attccgaaag attcgagagg attacccgct tgccggcgag ctggatcgcc
      901 ttgatcacct cgaagaactg tgcggtggcg gcgaagtcga tgcgcagcac cttgcccatg
      961 tcgacgacga gttcggtgcc ggacgcggcg cgcgcggcca gctcggcgaa ctcgccctgg
     1021 ctcgcggtgg aaaccacgcc cttcagcacg aagcccgctt ccggtgcgct gggcgctgcg
     1081 gtcacggcct gggtgcgggc ggtggcggcc gccacggggt tcacgtagac ctcccagttg
     1141 ggcggcgaga tctcgaaggc gacggcgtac tcgagtccca gttcctcgaa aggctccatc
     1201 ttgccctgca ccacgaagag ttcgaagagc agcatccagt agccgcgagt cgtatccgaa
     1261 gtccgctcgt tgaacgcggc gcgcagcagt tgctcgagcg aatccgtgtt gttgaaccac
     1321 atcggcatgc cggcgcggcg caggcgtttc agtgccgccg cgaggagcgc ggactcggcg
     1381 gcggtgatgg tggcgagctt gcccgtgtcg aggcgaaccg tccccatcga ctccgcgaaa
     1441 gcgacgaatt tctcgatctc ctgcgcgagc tcgcccgcgg cattgggccg gaggacaaac
     1501 aggtcgttgc gctcgcgatt ctgcgcgcgc cgcggatccg ttgcggcctc ggcgccgccg
     1561 gcccaggcgg gcggcgactg ctcgaacttc accgtgtaga cgagggagag ttcgtcgtac
     1621 gcgcggcgat tctgcgtggc ttcgtagagg tcgaacagca tgagccaagg ctgcttgttc
     1681 gagcggctcg aggggttgcg cgtctcgccc ttgaggatgg cctccgccgg ggccgcgaga
     1741 ttggcggcgt aaaggacggc ggcctcctgc gcatgcggca gcagggtgga gtggtaatcc
     1801 tcattgccga cgcgcgccgg ctggcggggc gctgacttca ggtttccggt cgacggaacg
     1861 ccggaggaag gggaagccac gatgtcgtgt gcctcagggc ctggccgggg aaccggtgaa
     1921 tccgctggcg acgcgtggaa tcggtccgta gggggacatg aatgaatgcc ttgtcgtttc
     1981 gagaggaagc ccgcgggttc gcgcgagcag gcccgcactt ccggagcgat gcaggcaaac
     2041 gccccgatcg cgaggaaggc ggccgccggc accgcgcccg tgagcgaggc ccagtatagc
     2101 gcccgtcacg gggagcgtca cgccttcccc agcgacgtga agcgtttccg ccgcaggcgg
     2161 ggccgcgctc acgccgcgca atacgcgagg ccttcctcga agaggcggcg cggaatcttg
     2221 cggccgatga acaccatcac gctctcgcgc gtctctcccg gcgcccacgg ctttccgggc
     2281 gtgccgccca tcagcatgtg cacgccctgg aagatcatgc gccgcggctc gccctggata
     2341 ttgaggaccc ccttgtagcg cagcaggtcg gcgccgtagt tctgcaccat gagcgacagg
     2401 aaggtctcga tcttctccat ctgcagcggc cgcgggtccc gccacacgaa gctcttcacg
     2461 tcgtcgtcgt ggtggtggtg gccctcggcg aggaagccgg ggtcgatctc gagcgcggcg
     2521 ttgagattga agccgcggat gtcgaggatt tcgcggatat ccgtttcgcc gaaatggacc
     2581 ttcttctggt gggcacgcgg attcatgtcg cgaagccggt ccatgaggtc ggccgtttcc
     2641 gcttccccga cgaggtccgt cttcgaaagc aggatccggt ccgcgaatcc cacctgcgcg
     2701 cgcgcctcgt cgtgctcgtc gagctgcttc tggccgtgct tcgcgtccac caccgtgatg
     2761 acggcgtcga gcaggtactc gtcgtggatg tcctcgtcca tgaaaaaggt ctgcgcgacc
     2821 gggccgggat tggcgagacc cgttgtctcg atcaccacgc ggtcgaactt cagcttgccc
     2881 tggcggcgct tcctgccgag gtcgccgagg atgcgcacga ggtcgccgcg cacggtgcag
     2941 cacaggcacc cgttgttcat ctcgacgatc tgctcctcgc ggtcctccac gaggatctcg
     3001 gtgtccacgc cggtctcgcc gaattcgttc tcgatgacgg cgatcctctc gccgtgctgc
     3061 tcggtgagga tgcggttcag gagcgtcgtc ttccccgcgc cgaggaagcc ggtgaggatg
     3121 gtgacgggga tgaggtcctt gtcgggttgc gcgacggcag ccatgcgtta cctttcgggg
     3181 caggggacgc ttccctcttg ctgcaaggcg gaatagggaa gcgtccccag ttccgagtcc
     3241 aacatctgtg cgacgaacga ttcttcaagg tcgcggacga tgaatacgag gcgcgttcgc
     3301 cggtcgccgt cgggccatga cggcaggggc gcggggggat agagcgtgtg ctgcacggcg
     3361 tgcacggcga cggggcccgc ttcgcccgcc acgttcacga gccccttcag gcgcaggatg
     3421 cgctccccgg ccaggtcgag aagggtggcg agggcgtcct cgaaggccgg ccaggagaca
     3481 ggggtcccgg cgaaccaggc gaacgcggtc acgcgcgggt cgtgcgcggc gggcatcggg
     3541 tcgcccaccc gccggtaggc gccggcgttc agccacgagc cggcgtccgc caggcgaacg
     3601 cttccgcggt acagccccgt atcgaggagg cgctgcgggt cggcgtcgcc gcgggcgctg
     3661 cgcaggcgca cggcgcctgg gttgagggcc gcaagccgcc cctcgagggc ctcgagcgcc
     3721 ggcggctccg cgagatccgt cttcgtgatc accaggcggt cggcgaccgc ggcctgcttc
     3781 gtggcctcgg gatggcgatc gagctggccg ctgccgtgct ggccgtccac ggtcgtcacg
     3841 atgccggcga gcgaatagcg ggccgcgacc agcggaagct cgatgagcgt gcgggcaaga
     3901 ggcgcggggt cggccagacc cgtggtctcg atgatcgcgc ggcggaacgc ggggatctcg
     3961 ccggcggccc gcttgaaata gaggtcgcgc aaggcctgca cgagatcgcc cgcgacctgg
     4021 cagcacacgc agccgctcgg caacagcacc acgttctccg aggccgtccg caccagcacg
     4081 tggtcgatcg ccaccggccc gaactcgttc acgatgaccg cgcagtcgcc gaattctggg
     4141 tgaccgagca gccggttgag cagcgtcgtc ttgccgctgc cgaggaagcc ggtgagcagc
     4201 gttaccggcg tgacggtggt ggcggtcatc ggcaaggcgg cccggcgggg atggcggtca
     4261 gtggcacgcg gcgcacagtc cctgcacggt gagctcgacc cgctgcggca ggaatcccct
     4321 cggcaggcgc acccgcggcg gaaccgcgac gtcctccagg cagacggtct tgccgcagcg
     4381 cgagcacgtg aagtgcgcat gcgggccatg cgagcggtcg cgattggcgt ggaagcgcca
     4441 ggtacggtcc tcgcccggga tgcggtgggc gagccccacg gcgaccagcc agtcgagcac
     4501 gcggtacacg gtcacgcggt cgacggcgag gtccgcccgg agcgcgccct cgagctcgtg
     4561 gtgcgtgagc gcggcctccg aaccgaggag tgcagcgagg accgccgcgc gcggggcggt
     4621 gagcctctct ccggcagcgc ggatccggac ctgcgccgcg ggcaggtgcg cggcgtaatg
     4681 cgttcgggac ttgcccatgg ccggattcta ggccgcgcga cgatccagat gcaattcggt
     4741 tgcgcggccg tcagcccttg cgcctggccc gcgggtgggc cgcgtcgtag accttggcca
     4801 ggtgctggaa atcgaggtgc gtgtagacct gggtcgaggc gatgctcgca tgaccgagca
     4861 tttcctgcac ggcgcgcagg tcacccgagg actgcagcac gtgcgaggcg aacgaatggc
     4921 gcagcatgtg cgggtgcaca tccatcgaga gccctgccgc ggccgcgcgg cgcttgatgc
     4981 ggcgctggac ctcgcgggtt gacatgcgct ttccggaacg gccgatgaat agcgcctttt
     5041 cgccaggtgc ggcgaggcgg tcccttaccg gcagccagcg ggacagcgcc tcgagcgccg
     5101 gttggcccac ggggacgatg cgcgtcttgg agccctttcc cgtgacgcgc gcttcgcccg
     5161 cgcccgcgtc cacggcgccc tggtcgagtc cggtgagctc ggagacccgc aggccgcagg
     5221 aataggcgag ctcgaagagc gcgcggtcgc ggatgccggc gtcggagggg tcatcgaagg
     5281 cgaccaggcg cgaggcgtcg tcgggggaaa gcgcctccgg aagcgtgcgc gccgcgcgtg
     5341 gcgcgcggat gcccttgcac gggttgaccg cgatctcccg gtgacgcacc agccagtcga
     5401 agaagccgcg ccacgaggaa agcagtcgcg cgagcgatcg cggcgaaagc ccctggccgt
     5461 gcagcacggc gacgaagcgg cggacatccg cggcgcgcag ttcgccgggc tcgcgtccct
     5521 ccgccagcgt cccgaggagc gccccatcgc gggaataggc ggtcagcgta tgggcggcga
     5581 ggcgccgctc gtgccggagg aaatcgaggt agcgcgcgag cgcgctagtc ggcgggctca
     5641 cggacggggg tgaggtggcg ccacagcgcg ctggagagga gctcgccgat gcgcgtgagg
     5701 tagagcgtgc ccatttcggg atagaagcgt tgcgcttcct ccgaggcgag cgcgagcacg
     5761 ccgaagcagt gctcgccgtg gcgcagcgcc acgagcgcaa aggacttcag gtgcggcgcg
     5821 gcctcgccga accagccctg gctttcgtag accgcgtggt tgccgcagta gggcgtggtc
     5881 atctgcgcca cgaactcgtg catctcggcg gcgacgtcgg cgaactcctt ggtctcgggg
     5941 tcgcgctcgg ccacgttcca caggcgcacc gcgacgtgcg gaaccgcgaa atggtcgagc
     6001 aggtcgagat aggtcgtgtc gagaacggca tcgagccccg tggcgagcat caggcggcag
     6061 gccatccggt ggaccttctc ggagacggca tcgttttcct cgccgaagcc gatgagctcg
     6121 cgaagcttgt cctcgaggag ccgcgtcttc tcgcgcgcgg cgacgagctg gcgttcgccg
     6181 atcgacaccg cgcgcccgcc gtgcgggtgg ggtatctgga tgttgatcag caggtcgacg
     6241 ttctgttcga agaaaccggg gttgcggcgg aggaattcgg cgacttgttc cggggtcagc
     6301 aagaggtggt ctccagggag ccggtcgccg ggagctcgat ttcgccctcg aagacgcgca
     6361 cggcattgcc cgtcatgata acgggagcga aggggggcgt catgtccgcc gccgtcccgt
     6421 cccagcggat ggcgagatca ccgccgcgcg tggtcacccg aacgcgtcgg tcgagaagcc
     6481 cccggcggat gccggcgacg acggcagcgc aggcgcccgt gccgcacgca agcgtctcgc
     6541 ccgcgccgcg ctcccagacg cgcaggcgga tgtggtcgcg cgcgaccacc tgcaggtagc
     6601 ccgcattcac gcggttggga aaagcggggt ggttctcgat gagcgggccc tgggtcgtga
     6661 ccggcgccgc atcgacgtcc gcgaccacct gcacggcgtg cgggttgccc atggacagca
     6721 cgctcgcctc gatcgtgatt cctcccacgg cgatcgcgta ggtcgggcgc tccgccggcg
     6781 ccgtgaaggg cacctcggcg ggcgcgaatc gcggcgggcc catgtcgacg ctcacctgcc
     6841 ccgtggcctc gatgcgcggg cggatgacgc cgcgcgcggt ctccacgcgc agctcggtct
     6901 tgtcggtgag cccctcgtcg cgcacgaatc gcgcgaaaca gcgcgccccg ttgccgcact
     6961 gctcgacctc gccgccgtcg gcgttccaga tgcggtagcg gaagtcgttg ccgggctcgc
     7021 gggccggctc gacgagcagg agctggtcgc agccgacgcc gaagtggcgg tcggcgatgg
     7081 cgcggacctg ggcagcggtc agcgcgacgg cctgccgcac gccgtcgatc acgacgaaat
     7141 cgttgccctg gccgtgcatc ttggtgaacc tgaggcgcat ggccgtcatt ttcgccgata
     7201 cggccctggc tcgccgggcg gacgcgtctt gaaccgcttg tgcggccaga aatactgctc
     7261 ggggatctcg cgcacgcgct cctcgatgaa ttcgttcatg cgccgcacgt cggcctcgat
     7321 gtcgccggtg ggatagtcct cccagggcgg gtagacgact atctcgtagc ctcgctcgag
     7381 ctggcgggcg attgccggca cgaccttcgc gcgcccgagc ttcgccaggc gcggaagcgc
     7441 ggtgaccgtg gccgccggca ccccgaagaa cgggacgaag atcgcgtcgc gcgggccgaa
     7501 atccatgtcg ggcaggaagt agagcaggcg tccctcgcgc agcgtcttga cgatggcgcg
     7561 catgccgtcc tgccgcgaaa gcaggatcgg ctcgccgaag cgcatccgcc cgtgcaggaa
     7621 aaggcggtcc aggaccggat tcttctgccg gctgtagatg ctcgcggagc cgggaaaatc
     7681 atttgcgatc cgggcgcccc ccatgttgag gccgatgaag tgcggcacca gcaggatgac
     7741 gggcgtgccc cggagccggt ccagatgctc gcgcccgcgg atcgccacga gatcgagcac
     7801 gcgcctgtcg ctcgcgaacc acatgacgct catctcgatc gcgttgcgac ccagcgcctc
     7861 gaaattgcgc cgaccgagcg ccttgcgctc ggcctcgggc atcccgggga agcatagggc
     7921 gaggttcgtg tccgtgacgc gtccgcgacc gaagcggtag agaagccttc ccagggcgcg
     7981 gccgatcgcc cccagcaccg gcgtgggaag ccagtgcatc agccacagca gggcccagcc
     8041 gagccacgat cccaggcggc gcaggatcac cattgcagcg cccccccagg tgccggcggc
     8101 gcgcctgccg gccgcttgta gcggttgtag ccccacaggt actgtgcagg cgcgatccgg
     8161 atgaggtcct cgatgccctg gttcacggcg agcgcggctg cagcccggtc tgcgggaagg
     8221 ggttcctcgc gggcgcgaag gtgcagccgg aatccccgcc cttccggcag gcgttcggcg
     8281 aagcagaaca cgaccgcggc gcccgaggct tcctgcagcc ggccggtcag ggtcatcgta
     8341 taggcggggc ggccgaagaa atcgacccat tcgccctcgc cctcgcccgg cacctggtcg
     8401 gggaggatca ccgtgcagcc tcccttgcgc agatggcgaa gaagcatgcg aacgcccgcc
     8461 atggtggctg gcgcgacgtt ggaaccgtcg ggtgccgcgc cgcgattgcg cccctcgcgg
     8521 atcacgtcgt cgagccagcc gagcttgtgc ggccggtaca tcaccaggat cggcaggcgc
     8581 gaccagaggt aacgcccggc tatgtcgtat ccgcccaggt gcggcgtgat gaaaatgagc
     8641 gggcgtccca gggcgcgcaa gcgctccacc gcgtcccagc cattcagttc cttcaccagc
     8701 cccgcgagct ccttcaccgg ccggaacagg gcccaggcga gttcggcggc ccccttgccg
     8761 atttcggccg cgttatcgcg ggccagtcgt gccgtgcctg cctcgtcggg agcgatgccc
     8821 gccgtgcgca ggttctcgag cgtgcgcaat cggtaccgcg gcgagaggag gaacacgagg
     8881 cggcccgcaa acgcacccag tcggtggttc gcggaaaggt ccagccaggc gaggaagcgg
     8941 agaaggagtt tcaaggcgcg gccgccggta aacggcgacg ggggcggata ttttgctaaa
     9001 atgccgggtt caattgcgac tgtggggcga acccgatgaa atcgaactac ctctttacct
     9061 cggaatcggt ttccgagggc cacccggaca aggtggcgga ccagatctcg gatgccgttc
     9121 tggacgccat tctagcccag gacccgcgct cgcgagtcgc ggcggagacc ctgtgcaaca
     9181 ccgggctcgt cgtgatggcc ggcgagatca cgacgcacgc gaacgtggac tacatccagg
     9241 tcgcgcgcga cacgatcaag cggatcggct acgacaacac cgatttcggc atcgactaca
     9301 agggttgcgc ggtcatggtc tgctacgaca agcagagccc ggacatcgcc gcggccgtgg
     9361 atcacgcctc cgacgaatac ctcaaccagg gggccggcga ccaggggatg atgttcggct
     9421 acgcgtgcga cgagacgccg gtactcatgc ccgcgccgat ctactacgcc caccgcatcg
     9481 tcgagcggca gtcgcaggtg aggaaggacg ggcggctcgc ctggctgcgt cccgacgcca
     9541 agagccaggt gacgatgcgc tacgagaacg gcaagcccgt ctccatcgac accatcgtgc
     9601 tctccaccca gcatgcgccg gacgtctcgc aggaacagat caaggaggcg gtgatcgagc
     9661 tggtcatcaa gcccgcgctg ccgaaggaga tgctcaagga cacgcgttac ctggtgaatc
     9721 cgagcgggcg cttcgtcgtg ggcggaccgc agggcgactg cggcctcacc gggcgcaaga
     9781 tcatcgtcga cacctacggc ggcgcctgcc cgcacggcgg cggcgcgttt tccggcaagg
     9841 atccgtcgaa agtcgaccgc tccgccgcct acgcggcacg ctacgtggcc aagaacgtgg
     9901 tggccgcggg actcgcacgc cagtgccaga tccagctctc ctacgcgatc ggcgtggcca
     9961 agcccatcaa cgtgaccgtg tacaccgaag gcacgggcgc catcgccgac gaaaagatcg
    10021 cgaagctggt cgaggagcac ttcgacctgc gccccaaggg catcgtcacg atgctcgacc
    10081 tcctgcgccc catctacctg aagaccgccg cctacggcca cttcggccgc gaggagccgg
    10141 aattcatgtg ggagaccacg gacaaggcga agctgctgcg cgacgcggcc ggcctcaagg
    10201 gtgcgcacgc gaaggcgacg gcgaccgcgg actgaagggt tccggtcccc cgggcgtgac
    10261 gaccggaatg aaatgagcac gggcggccag ttggccgccc gtgctcattt catgcggtag
    10321 cccgaatggc aagccacgca ttgctgcgtg aggcgtgcca gggctgcgag ggccggcttc
    10381 acgttgcccg tcgcgccggc gttggcggcc tccacggaaa agcggctcgc cgaacggtgc
    10441 atctcggtcc cggcggcctg catgccaacg ggcatgaact tcgccaggtc gtgggcgccg
    10501 tgtgcggtga gcgacgtcat gccgaggcgc ttctccgaaa cgtccgccgc ccggtcgaag
    10561 gcgctctcgg cgagggcctg ctggatctcc tgcagcgccg cgaggtggtc gcgcatgttg
    10621 gccagggtgt gcgagcgcag cggctcgggg aaatcgacgg ccaagcgaga gtccggagcc
    10681 ccggcttcat ggcgcgcggt gtcgtggacg ggtccggcgg cacagccggc cgcaaggcag
    10741 gccgcggaga
//
LOCUS       DASUEV010000092         6012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_713459, whole genome shotgun sequence.
ACCESSION   DASUEV010000092 DASUEV010000000
VERSION     DASUEV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713459"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1373
                     /gene="zapE"
                     /locus_tag="VFV55_02915"
     CDS             156..1373
                     /gene="zapE"
                     /locus_tag="VFV55_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX4943275.1"
                     /translation="MTDTAETFPVEDEELEPADPNQYSGPLEWYWHFQQRPDFETDSE
                     QLRVLGELERLFGELEDYRQYRAGRLNRLVTNLGAGRKPPRGMYIWGGVGRGKSLMMD
                     AFFKVSRHRRKRRVHFHEFMREIHGRMRALSGTEDPLDAISSEIARELRLLCFDEFHV
                     GDIADAMILGRLLELMIAKGVVFVMTSNYGPDGLYPNGLQRARFLPAIEIIKRELDVI
                     AMGAGSDHRRRILEGFRVWYSPPGEEADQHLALFFEAMTKAAFERDDSIEIGGRALPF
                     RQRAKGVIWFDFEALCMEPRSQLDYLEIASGYHTVLVSGVPRLLPAQTDVVRRLTWLV
                     DVLYDQRVRLVMSAEVPPEELVAPSVELTGPGRIVRAEFARTASRLREMQSRDYFARK
                     HASAENPQVLRAR"
     gene            1433..2416
                     /locus_tag="VFV55_02920"
     CDS             1433..2416
                     /locus_tag="VFV55_02920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEX4943276.1"
                     /translation="MFKAIHLTREADQFQARVAQLDEAALPEGDVTLRVEYSTINYKD
                     ALAITNKSPVVRKWPMVAGIDGAGVVEQSSHPAWKPGDRVILDGWGVGEAHWGCLAQK
                     ARLKGDWLVRLPPGFTTRQAMAIGTAGYTAMLCALALAKQGVAKDQGDILVTGATGGV
                     GSVAISILSGWGYRVVASTGKAAEADYLRSLGAADVIDRNELAQPGKPLQKERWAGVV
                     DSVGSHTLANACAQMKYRGVVAACGLAQGMDFPASVAPFILRGVRLIGVDSVMAPRGE
                     REEAWARLASDLDRAKLDAITTEVGLAEAIARAPDVLAARVRGRIVVDVNK"
     gene            2442..3407
                     /locus_tag="VFV55_02925"
     CDS             2442..3407
                     /locus_tag="VFV55_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4943277.1"
                     /translation="MKRSIAAIAGLVLAFAAPVAWSQAWPTKPIRILVGVTPGGTTDT
                     LARFLGQEMAKELGQPVVVENRAGAGGNIAAEAVARSSPDGYTLLFVNTSHSVNMGLY
                     GSRLPYDAVKDFTPITQVSTGPAVLVAARDFPAKDANDVIAMAKAQPGKLDFAIGGLG
                     TSIHMAGELFKMMAGVDIANIPYKGSSPALADVMGGQVKLMFAPVINAVPQVKAGKVK
                     ALGVTSTTRIPALPDVPPIADAVPGYESAAYFGLLGPAKLPRDVLAKLHASAARAAQS
                     AELKARLEPDGSRVVGGTPEEFTAFLVADIEKWRRVVKATGARPD"
     gene            3530..5425
                     /locus_tag="VFV55_02930"
     CDS             3530..5425
                     /locus_tag="VFV55_02930"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEX4943278.1"
                     /translation="MTTYKEFHARSIRDPEGFWGEQAKLIDWHRPFDKVLDYSKPPFS
                     PWFSGGLTNLCHNAVDRHLASRAAQKALVWISTEVNEEKSWTFAELHDEVNCFAAILR
                     SLGVGKGDRVLIYMPMVPEAVFAMLATVRIGAIHSVVFGGFAAASLAARIDDAQPKVM
                     ITSDAGARGGKPIPLKPLVDESIRLSKAPPAHVVIVNRRLDAAMTTVPGRDLDYAELR
                     AKHAGAKVPVTWLESNEPSYILYTSGTTGLPKGVQRDTGGYAVALAASMKHIYCSKPG
                     EAYFSTSDIGWVVGHSYIVYGPLINGSTTIMYEGLPIRPDPGIWWKIVSDYKVTSMFS
                     SPTAIRVLKKQDPAYMSKHDTKCLKYLFLAGEPLDEPTARWVADNLGCPVVDNFWQTE
                     TGWPILSACLGVEDTPRKFGSPSFPVYGYNVKIKDEITGKETGPDEKGVLCIVPPLPP
                     GVMTTVWGDDERYVKTYYGSFRDEMVYTTFDWAVRDKDGYYFVLGRTDDVINVAGHRL
                     GTREIEEAVQAHAGIAEVAVVGVADSLKGQVPVAFAVAKDATKTATKDLAAAFEKEVM
                     AMVDKVLGAIGRPARVHFVTVLPKTRSGKLLRRSIQALCEGRDPGDLTTIEDPGALEQ
                     IRAALRG"
     gene            5422..5934
                     /locus_tag="VFV55_02935"
     CDS             5422..5934
                     /locus_tag="VFV55_02935"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEX4943279.1"
                     /translation="MTGPLVPDVLAPGLRLVFCGTAPSKASAAARAYYAKPGNRFWKA
                     LHEAGFTPRRFLPAEYAKLLELGIGLTDLCKGHSGNDDELPRGSLDAPGLLRKIERFR
                     PGVVAFTSKNAAKAFLGRDVDYGWQPERVGETRLFVLPSPSGQATRFWDPRPWRELAE
                     VKGSGYFAQK"
BASE COUNT         1004 a         1976 c         2089 g          943 t
ORIGIN      
        1 cgttcgacac ggatgaacac ggatgaacac ggataagggc agatgagagt gtttcaggat
       61 tcgaggcatg ttcattgcag gtccaggaaa cgtgatttat ttgcttttat ccgtgttcat
      121 ccgtgttcat ccgtgtcccc cgcttttggg ttccgatgac cgacaccgct gaaaccttcc
      181 cggtcgagga cgaggaactc gagcccgcgg atccgaacca gtactcgggc ccgctcgagt
      241 ggtactggca cttccagcag cgccccgact tcgagaccga cagcgagcag ttgcgcgtgc
      301 tgggcgagct ggagcggctc ttcggcgagc tggaggacta ccgccagtac cgcgcgggaa
      361 ggctgaaccg cctggtgacc aacctcggcg ccggccggaa gcccccgcgc ggcatgtaca
      421 tctggggcgg cgtggggcgc ggcaagtcgc tcatgatgga cgccttcttc aaggtgagcc
      481 gccaccggcg caagcgccgc gtccacttcc acgagttcat gcgcgagatc cacggccgga
      541 tgcgcgcgct ctcgggcacc gaggatccgc tggacgccat ctcgagcgaa atcgcgcggg
      601 aactgcggct cctctgcttc gacgaattcc acgtcggcga catcgccgac gcgatgatcc
      661 tcgggcgcct gctggagctg atgatcgcca agggcgtcgt cttcgtgatg acctccaact
      721 acgggcccga cggactctac ccgaacgggc tgcagcgcgc gcggttcctg ccggccatcg
      781 agatcatcaa gcgcgagctc gacgtgatcg cgatgggcgc ggggtccgac caccggcggc
      841 gcatcctgga gggcttccgc gtgtggtatt cgccgccggg agaggaagcc gaccagcacc
      901 tcgccctctt cttcgaggcg atgaccaagg ccgccttcga gcgcgacgac tcgatcgaga
      961 tcgggggccg ggcgctgcct ttccgccagc gcgcgaaggg cgtgatctgg ttcgatttcg
     1021 aggccctgtg catggagccg cgctcgcagc tcgattacct cgagatcgcg agcggctacc
     1081 acacggtgct ggtgtccggc gtgccgcgcc tgctgcccgc gcagacggac gtggtccgcc
     1141 gcctgacctg gctcgtcgac gtgctctacg accagcgggt gcggctggtg atgtcggcgg
     1201 aggtcccgcc cgaggaactc gtggcgcctt cggtggaact caccggtccc gggaggatcg
     1261 ttcgcgccga gttcgcgcgc accgcgagcc gcctgcgcga gatgcagtcc agggactatt
     1321 tcgcgcgcaa gcacgcttcg gcggagaatc cccaggtgct gcgggcgagg taattcgcgg
     1381 ccggggccgg cgtccggtac cgtggctcat caccatcgga gacattgcaa ccatgttcaa
     1441 ggccatccat ctcacccgcg aagcggacca gttccaggcg cgcgtcgcgc agctcgacga
     1501 agccgcgctg ccggaaggcg acgtgacgct gcgcgtcgag tactccacga tcaactacaa
     1561 ggacgctctc gcaatcacca acaagtcgcc cgtggtgcgc aagtggccca tggtcgccgg
     1621 catcgacggc gcgggcgtgg tcgagcaaag cagccatccg gcgtggaagc cgggcgaccg
     1681 ggtgatcctc gacggctggg gcgtgggcga ggcgcactgg ggatgcctcg cgcagaaggc
     1741 gcggctcaag ggcgactggc tggtgcgcct gccgccggga ttcacgacgc gccaggcgat
     1801 ggcgatcggc acggccggct ataccgcgat gctctgcgcg ctggcgctcg cgaagcaggg
     1861 cgtggcgaag gaccagggcg acatcctcgt gaccggggcc accggtggcg tgggcagcgt
     1921 ggccatctcc atcctgtcgg gctggggcta ccgggtcgtc gcctcgacgg gaaaggccgc
     1981 ggaggcggat tacctccggt cgctgggcgc ggccgacgtg atcgaccgca acgagctggc
     2041 tcagccgggc aagccgttgc agaaggagcg ctgggccggc gtagtcgact cggtgggcag
     2101 ccacacgctt gccaacgcct gtgcgcagat gaagtaccgc ggcgtggtcg ccgcctgcgg
     2161 gctggcgcag ggcatggatt ttcccgcgag cgtcgcgccg ttcatcctgc gcggcgtgcg
     2221 gctcatcggc gtggacagcg tcatggcgcc gagaggggag cgcgaagagg cgtgggcgcg
     2281 ccttgcgtcc gacctcgatc gcgccaagct cgatgccatc acgacggagg tgggtcttgc
     2341 cgaagccatt gcacgggcgc cggatgtcct ggcggccagg gtccgcggcc gcatcgttgt
     2401 ggacgtgaac aaatgaccag gtccgcgtgg agaaccggaa aatgaaacgc tcgatcgccg
     2461 caatcgcggg gctggttctg gccttcgcag cccctgtcgc ctggtcgcag gcgtggccca
     2521 cgaagccgat caggatcctg gtaggcgtga cgcccggggg caccaccgac acgctggcgc
     2581 gattcctcgg gcaggagatg gcgaaggaac tcgggcaacc ggtcgtcgtg gaaaaccgcg
     2641 ccggcgcggg cggcaacatt gcggcggagg cggtcgcccg ctcgtcgccg gacggctaca
     2701 cgctcctctt cgtgaacacg agccacagcg tgaacatggg cctctacggg tcgcggctgc
     2761 cttacgacgc tgtgaaggac ttcacgccca tcacgcaggt gtcgacgggg ccggcggtgc
     2821 tggtggcggc aagggacttt cccgcaaagg acgcgaacga cgtgatcgcc atggccaagg
     2881 cccagcccgg aaagctcgat ttcgcgatcg gcgggctggg cacctccatc cacatggcgg
     2941 gcgagctctt caagatgatg gccggcgtcg acatcgccaa catcccgtac aagggcagct
     3001 ccccggcgct cgccgacgtg atgggcgggc aggtgaagct catgttcgcg ccggtgatca
     3061 atgcggttcc gcaggtgaag gcgggcaagg tgaaggcgct cggcgtgacc agcaccacgc
     3121 gcattccggc gcttcccgac gtgccgccga tcgccgatgc cgtgcccggc tacgagtcgg
     3181 ccgcgtattt cggcctgctc gggccggcga agctgcccag ggacgtcctc gcgaagctgc
     3241 acgcgtccgc ggcgcgggcg gcgcagtccg ccgaactcaa ggcgcgcctc gaaccggatg
     3301 gctcgcgggt ggtcggcgga acgccggagg agttcacggc tttcctcgtc gccgatatcg
     3361 agaagtggcg gcgggtggtg aaggccacgg gcgcaaggcc cgactaggga ataccctgac
     3421 ccgcgcacct gatactttgc ggcggatcaa ctaagctcgc cgccggaaat ctaagctgca
     3481 agtcgccccg ccgaagccgg gccggcccat tccgccagga gacgatccga tgaccaccta
     3541 caaggaattc cacgcgcgct cgattcgcga tcccgagggt ttctggggcg agcaggcgaa
     3601 gctcatcgac tggcaccggc ccttcgacaa ggtgctcgac tacagcaagc cgccgttcag
     3661 tccgtggttc tcgggcggcc tcacgaacct ctgccacaac gccgtcgatc gccatctcgc
     3721 ttcgcgcgcg gcgcagaagg cgctggtctg gatctccacc gaggtgaacg aggagaagag
     3781 ctggaccttc gccgaactcc acgacgaggt gaactgcttc gcggcgatcc tgcgctcgct
     3841 cggcgtcggc aagggcgacc gcgtcctcat ctacatgccg atggtgccgg aggcggtgtt
     3901 cgccatgctc gccacggtgc gcatcggcgc catccattcc gtggtgttcg gcggctttgc
     3961 ggcggcgagc ctcgcggcgc gcatcgacga cgcgcagccg aaggtgatga tcacgtcgga
     4021 cgccggtgcg cgcggcggca agcccatccc gttgaagccc ctggtcgacg aatcgatccg
     4081 gctctcgaag gcgcctcccg cccatgtcgt catcgtgaac cggcggctcg acgcggcgat
     4141 gacgacggtt cccgggcgcg acctcgacta cgcggagctt cgcgcgaaac acgccggcgc
     4201 gaaggtgccg gtgacgtggc tggagtcgaa cgagccttcc tacattctct acaccagcgg
     4261 aaccaccggg ctgcccaagg gcgtgcagcg cgacacgggc ggctatgcgg tggcgctcgc
     4321 ggcgtcgatg aagcacatct actgctcgaa gccgggcgag gcgtacttct ccacctccga
     4381 catcggctgg gtcgtgggcc attcctacat cgtctacggg ccgctcatca acggctcgac
     4441 cacgatcatg tacgaggggc tgccgatccg ccccgacccg ggcatctggt ggaagatcgt
     4501 gtccgactac aaggtcacct cgatgttctc ctcgcccacg gcgatccggg tgctcaagaa
     4561 gcaggacccg gcctacatgt cgaagcacga cacgaaatgc ctcaagtacc tcttcctcgc
     4621 cggcgagccc ctggatgagc ccaccgcgcg ctgggtggcg gacaacctgg gctgcccggt
     4681 cgtggacaac ttctggcaga ccgagaccgg ctggccgatc ctgtcggcgt gcctcggcgt
     4741 ggaggacacg ccgcgcaagt tcggctcgcc ctcgttcccc gtgtacggct acaacgtgaa
     4801 gatcaaggac gagatcaccg gcaaggagac gggccccgac gagaagggcg tgctttgcat
     4861 cgtgccgccc ctgccgccgg gcgtgatgac gacggtgtgg ggcgacgacg agcgctacgt
     4921 gaagacctat tacgggtcct tcagggacga gatggtctac acgaccttcg actgggccgt
     4981 acgcgacaag gacggctact acttcgtgct cgggcgcacc gacgacgtga tcaacgtcgc
     5041 cgggcaccgc ctcggcacgc gcgagatcga ggaggcggtg caggcccatg ccggcatcgc
     5101 ggaggtggcg gtggtgggcg tggccgactc gctcaagggc caggtgccgg tcgccttcgc
     5161 ggtggccaag gatgcgacga agaccgccac gaaggacctg gccgcggcgt tcgagaagga
     5221 agtcatggcc atggtggaca aggtgctcgg cgccatcggg cggccggccc gggtgcattt
     5281 cgtgacggtg ctgcccaaga cgcgctcggg caagctgctg cggcgatcca tccaggcgct
     5341 gtgcgaaggg cgcgatccgg gcgatctcac gaccatcgag gatccgggcg cgctcgagca
     5401 gatccgcgcc gcgctgcgcg ggtgacggga ccactcgtcc ccgacgttct cgcgccgggg
     5461 ctgcgcctcg tcttctgcgg cacggcgccc tcgaaagcgt ctgccgcggc gcgagcgtac
     5521 tacgcgaaac cggggaaccg gttctggaag gcgctgcacg aggcgggctt cacaccgcgg
     5581 cgcttccttc ccgccgagta cgcgaagctg ctcgagctgg ggatcggcct caccgacctg
     5641 tgcaagggac actcgggcaa cgacgacgag ttgcctcgcg gttctctcga cgcacccggc
     5701 ctgctcagga agatcgagcg cttccgcccc ggtgtggtgg cattcaccag caagaacgcc
     5761 gccaaggcct tcctcggccg cgacgtcgac tacggctggc agcccgagcg ggtaggggaa
     5821 actcgtctct tcgtgctgcc gtcaccgtcc gggcaggcga cccgcttctg ggacccgcgt
     5881 ccctggcggg agctcgcgga ggtaaagggg tcaggttact ttgcacaaaa atgaggcgat
     5941 gaaaaagggg gcaggttact tttcaatggt tcctgccgtc gacagacggc tggctgaaaa
     6001 gtaacctgcc cc
//
LOCUS       DASUEV010000093        10155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1617068, whole genome shotgun sequence.
ACCESSION   DASUEV010000093 DASUEV010000000
VERSION     DASUEV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617068"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..562)
                     /locus_tag="VFV55_02940"
     CDS             complement(365..562)
                     /locus_tag="VFV55_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943280.1"
                     /translation="MSPNQRKQWKPPVRLDSVANAFVLAAALLVVLFAAGTAHFDAEP
                     LRMADRQAEVRIVADASSPTR"
     gene            734..3136
                     /locus_tag="VFV55_02945"
     CDS             734..3136
                     /locus_tag="VFV55_02945"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4943281.1"
                     /translation="MPDCYSFGAVEVRPAERRLLIDGRPAAVGARAFDFLLALIDRRD
                     RVVSKDELLDIVWRGLVVEENNLQVQVSTLRRLLGPRAIATVPGHGYRFVLHLDEPRD
                     ATPVSPRKENLPVQLDSFIGREREIRELEGLLAAAPLVTLTGTGGTGKTRLSLQLAAG
                     LVDGHPDGVWLVELAPLADARLVAQAIAAVLGVKEAAGRPVSEALAKHVASRHLLLIL
                     DNCEHLIEGCAEIASSLLRASPRLKILATSREPLRVRGETTYPLAPLPTPAGEDPADV
                     EAITQCDAVRLFVDRAIAAQPAFAVTQRNATAVAEICRHLDGIPLAIELAAARVRALD
                     VETIAGRLGDRFRLLAGGDPTALPRQQTLRALIDWSHDLLTAPERALLRRLAVFAGSW
                     TLQAAESVASGNGIDEPGVLGLLASLVEKSLVAREVEGTRYRLLETVRQYALERLDDS
                     GEAGAIRTRHLHCCLALAEEAAAGIIGPDQGTWLARLDVERENLLAAHAWCDRSEGGA
                     EAGLRLVCALKRYWINRGLPGLAHRMAVEALTRPGAEARTLARCRALFDAGQLDSWMG
                     RYGEAQAYLVESLAIARETGDRSRIEAVLQPLGLAMLGKGDLRAARGHFEEALALARE
                     LGNKRELAAALNALAQLHRAEGHPDAAEPLYEQAVDLARELGDRESIAIGLLNLAMVS
                     IGRGHRGRAPAMLLEVIGIAGEIGSKPVGQSVLDVCAGLAALRGEPDRAAIFYGAAQE
                     QAKRTGLRRDPADEAFLAPLVEKARGVLGDAAFAAAQAAGRESGFEESMGLARAWVTR
                     SR"
     gene            complement(3120..3536)
                     /locus_tag="VFV55_02950"
     CDS             complement(3120..3536)
                     /locus_tag="VFV55_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943282.1"
                     /translation="MKTRKNALIGLALIFGLTACAHERVTHFGRPDPHNPRVHIAAGS
                     FVVVDQEPIYVSLKEKNFLITWHLPAVSGYVFPDDGIVIAKPDGEFDCKVQPGGERFS
                     CTFRNSKPGRYKYTIKVLDREKGKLLEPLDPFIVNG"
     gene            3707..4996
                     /locus_tag="VFV55_02955"
     CDS             3707..4996
                     /locus_tag="VFV55_02955"
                     /inference="COORDINATES: protein motif:HMM:NF020322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4943283.1"
                     /translation="MFESLQSALATNKFVSGGIVLAALGVAAMWLREVPVKVARWAKH
                     FFVTTLSIDSREELMFPALVEYMDTREALRRLNNFTVRTVRQQDSSYQSLHDELQQGG
                     RPQTLFSPGEGFHLFVLDGRLMWMKREVQVGVSIVEKITLSTLGRDKRPLEALVHAAM
                     EHRIERELNRIAIHVPSTYGNDWTRARLGNNRKLQSVVLKAGQKEAILADLERFFASR
                     ERFFASRERYETLGIPWRRGYLLYGPPGTGKTSLVTALASELSLNVCVLSLASPNITD
                     EKVGNLLASVPRRSVILIEDVDAFFQNRSKADAQVRVSYSGFINALDGVTAHEGSVVF
                     LTTNHPQLIDEAAIRSGRVDFRLELGLCDRDQLERMFTKFIHDPVAAARFADAVQADR
                     WSPAQVQERLLKAGSVDEALELFRERAEGEVAMLRAA"
     gene            complement(5021..5524)
                     /locus_tag="VFV55_02960"
     CDS             complement(5021..5524)
                     /locus_tag="VFV55_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX4943284.1"
                     /translation="MKLPPKDIVETTLANRTLTTLSSAMAAAGLVKQFQGPGPFTLFA
                     PSDEAFLKLPGEKLDALMRDKKRLAALINYHTLRGTMKTSDIPNGTAKTVEGGMLTIG
                     ATDEGMRVDHANVTTPNIASRNGVIHVIDTVMMPRAEGVAPPTPDLHESPWSGRRRAP
                     PRPRSRW"
     gene            complement(5756..7564)
                     /locus_tag="VFV55_02965"
     CDS             complement(5756..7564)
                     /locus_tag="VFV55_02965"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943285.1"
                     /translation="MNLKKLTLALAALAALPFAVQSAEPETEAVEYYNLTTNHYFITA
                     TASEARIIDDGGAGPGWVRTGRSFQAWLDPAKAPSTASEVCRFYSTGANSHFYTGDAA
                     ECASLKSLEAAERSSAQATGAPILGWRYEGIGFRIEMPASGSCPAGTLAISRVYNNGF
                     TSGEGSNHRFVDDSQLRDLMVDRSWVAEGVAICARAKPNGSDANLGPTTTNFDPLAAA
                     WTGTARWKKEAGAVETRVAAPLALTIAADGKVDGSGQGCAFTGQVQSGDGFRSLFSGT
                     ISASGCTDAAFSGAYSRFQLERFGNGTLAVHMKRGDNANEASVEAVLTTGAPPVTPPP
                     STNPSAGGITGDWAGTVGWTALQRQGGTETFLVESNRPLQLAISSGGAIAGSGFGCSF
                     SGTLSTTSVAGIFGGSVSASGCTEGVFNGTYATVKVKRDDAMLEVEFEKESETAGVTT
                     KVQVEGKLVSTAVLPTTPVNPPSPPAGIAGTYKSAFTADIEIRDRSNGSDNTTTSSLS
                     SESQFNVTTGGGVSGTGFACSFNGTLTLTDAALQLHTGTITATGCSSASLNGSYTASA
                     HPENGGALQLEMERESEVANVRTKVRIHGTAPRTGA"
     gene            complement(7913..9487)
                     /locus_tag="VFV55_02970"
     CDS             complement(7913..9487)
                     /locus_tag="VFV55_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4943286.1"
                     /translation="MQDQPFKLTYSTMFDPPEALHQRFEATLAEVKGRLGAPCPMRIG
                     GEARAGRAEFESRSPIDRDWLLARLPRGTAQDAADAVAAARAAFRPWARTAWPERVAL
                     LRRVAKRIEERVYELGAAMALEVGKNRMEAIGEVQETADLISWYCAEMEENGGFLRYL
                     PDDPIAGFTSRNRTVLRPHGVWVVIAPFNFPMALAGGPIGAALVAGNTVVFKVASDTA
                     WCGALLMDVFREAGIPDGVLNYVTGSGAEIGEALVRHPDVAGITFTGSGEVGMRIVRL
                     FGEGRWPRPCIVEMGGKNPVVVTRHADLGRAATGIFRSAFGLQGQKCSAASRVLAERP
                     VMDELVARLAQLAGEAAVGDPTLRRNWLGPVINAAARERYRKVVAEVADGGRVYAGGR
                     ELADGDLARGYFVAPTVATAPWASRLWRDEHFLPFVLVGAVDSLDEALTRANDTDYGL
                     TAGFYGAPGEIDTFLERIEAGVVYVNRPQGATTGAWPGYQPFGGWKGSGSTGKAIGSF
                     YYVTQYLREQSQTVVE"
     gene            complement(9511..9903)
                     /locus_tag="VFV55_02975"
     CDS             complement(9511..9903)
                     /locus_tag="VFV55_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX4943287.1"
                     /translation="MKKTKPFPVPVRVLYSCAKCPAYCCTYSDIEVGKRDIARLARHF
                     GLDYETAEARFTTLDPKEKVRLLRHRSDKVFDSACTFLDQKLRRCTVYEARPGVCRNY
                     PDSRRCGYYDFLKFEREQQGDPEFVART"
BASE COUNT         1604 a         3435 c         3453 g         1663 t
ORIGIN      
        1 ctctcaatgg gtaaaaagtc ggggacagac gtgtttggct ggccccgact ttttgggacc
       61 cattcgacgg tcccgcttgc gtgcccagga cgccgattaa cttgccccat ccccggcctg
      121 actccaattc gccgttcgcg catgcgtgcc caggacgccg atggacttgc cccatcgccg
      181 gcccatctcc ccttcgccgc gcgcatccgg cactctggtc gcatcccgcg atgctgaccg
      241 cgacacgctt gcgcggaatt cggcgatgat cgaaaaacag ggcgagggcg acccacaacc
      301 gcggccgtcc ccgccaaaca cgcgcctcac gagtgtgcga gccttgtgga gcgatttctt
      361 gatgtcatct cgttggcgaa gacgcgtctg ccacaatgcg aacttccgcc tgccggtccg
      421 ccatgcgtag cggctcggcg tcgaaatgcg ccgttcccgc cgcgaacagg acgacgagca
      481 atgcggcagc cagcacgaag gcgttggcga cgctgtcgag cctcacgggg ggtttccact
      541 gcttgcgttg attcgggctc atgacgggct ccttggaagg gcggtgccgg cactgcatgg
      601 ccggtgaaca gactctccgc cccgcggggt ccggccgtcc ttaagccggc ctgaaaagat
      661 cctgaaatcc gggagaaggt tagaatttcc cggcggctga ttgcgcttcc aggcctctcc
      721 cagggagaag cacttgcccg actgctacag cttcggcgcc gttgaagtac gcccggcaga
      781 gcggcggctg ctcatcgacg gacggccggc cgccgtgggc gcccgcgcct tcgacttcct
      841 gctggcactg atcgatcgcc gcgaccgcgt ggtgagcaag gacgagttgc tcgacatcgt
      901 ctggcgcggc cttgtcgtcg aggaaaacaa cctccaggta caggtgtcca ccctgcgccg
      961 gctcctgggc ccgcgagcca tcgccaccgt ccccgggcac ggctaccgct tcgtgctcca
     1021 tctcgatgag ccccgggacg cgacgccggt ttctccacgc aaggagaacc tgccggtgca
     1081 gctcgacagc ttcatcggcc gcgagcgcga gatccgggag ctggaaggcc tgctcgccgc
     1141 ggcgccgttg gtgaccctga ccggcacggg cggcaccggc aagacgcgcc tgtcgttgca
     1201 gctggccgcc ggactggtag acggacaccc ggatggcgta tggctcgtgg aactggcgcc
     1261 gctggccgat gcgcggctgg tggcacaggc catcgccgcg gtgctcggcg tgaaggaggc
     1321 ggcggggcgc ccggtatcgg aggccctggc gaagcacgtg gccagccggc acctgcttct
     1381 catcctggac aactgcgagc acctcatcga gggttgcgcc gaaatcgcca gctcgctgct
     1441 gcgagcgagc ccgcgcctga agatcctcgc caccagtcgt gaacccttgc gcgtcagggg
     1501 cgaaacgact tatccgctgg cgccgctccc gactcccgcc ggcgaggatc cggccgatgt
     1561 cgaagccatc acgcaatgcg atgccgtccg cctgttcgtc gatcgcgcga tcgccgcgca
     1621 gcccgccttc gcggtgacgc aacgcaatgc gacggccgtg gccgaaatct gccgacacct
     1681 ggatggcatc ccgctcgcga tagaactggc ggcggcgcgc gtacgcgcgc tcgacgtgga
     1741 gacgatcgcc ggccgcctgg gagatcgctt ccgcctgctc gcgggaggcg accccaccgc
     1801 gttgccgcgc cagcagacgc ttcgcgccct gatcgactgg agccatgacc tgctgaccgc
     1861 acccgagcgc gccctcttgc gacggctcgc cgtgtttgcc gggagctgga ccttgcaggc
     1921 cgccgaatcg gtggcctccg gaaacggaat cgacgagccg ggcgtgctcg ggctcctggc
     1981 ttccctggtg gaaaagtcgc tcgttgcccg ggaagtggaa ggtacgcgct atcgcctcct
     2041 cgaaacggtt cgccagtacg ccctggagcg gctcgacgat tcgggcgagg cgggcgccat
     2101 ccggacgcgc cacctgcact gctgcctggc actcgcggaa gaggcggccg ccggaatcat
     2161 cgggcccgac caggggacgt ggctcgcgcg gctagacgtc gaacgggaaa accttctcgc
     2221 cgcgcacgcc tggtgcgatc ggtcggaggg aggagccgaa gccgggttgc ggctcgtctg
     2281 cgcgctgaag cgctattgga tcaaccgcgg cctgccagga ctcgcgcacc gcatggccgt
     2341 cgaggctctc acgcgccccg gggccgaggc acgaaccctc gcccgctgcc gcgcgctctt
     2401 cgacgcgggc cagctcgact cgtggatggg ccgctatggc gaggcgcagg catacctggt
     2461 ggaaagcctc gcgatcgccc gggaaaccgg tgaccgaagc cggatcgagg cggtgctcca
     2521 gccgctgggc ctggccatgc tcggcaaagg ggacttgcgc gccgcccgcg ggcatttcga
     2581 ggaagcgctt gccctggcgc gggagttggg caacaagcgc gaactcgccg ccgcgctcaa
     2641 cgcgctggcg cagcttcacc gtgcggaggg ccatcccgat gccgccgaac ccctctacga
     2701 gcaggccgtg gacctggcgc gcgagctggg tgaccgggaa agcatcgcga tcggcctgct
     2761 caacctggcg atggtgtcaa tcggccgcgg gcaccgtggc cgtgcccccg ccatgctgct
     2821 cgaggtgatc ggcatcgcag gagagatcgg ctcgaagccg gtcgggcaaa gcgtccttga
     2881 cgtgtgcgcg gggctggcgg cattgcgcgg ggagccggat cgcgccgcca ttttctacgg
     2941 cgccgcccag gaacaggcga agcggaccgg gctgcgccgc gaccctgccg acgaggcgtt
     3001 cctcgcaccg ctggtcgaga aggcgcgtgg ggtgttgggc gacgccgcgt tcgcggcggc
     3061 gcaggccgcc gggcgcgaaa gcggcttcga ggaatcgatg gggctggcgc gcgcctgggt
     3121 cacccgttca cgatgaacgg atcgagcggc tcgagcagct tgcccttttc cctgtccagc
     3181 accttgatcg tgtacttgta gcgccccggc ttcgaattgc ggaacgtgca cgaaaaccgc
     3241 tccccgcctg gttgcacctt gcaatcgaat tcgccgtcgg gcttggcgat cacgatgccg
     3301 tcgtcgggaa acacgtagcc ggagaccgcg ggcaggtgcc acgtgatcag gaaattcttt
     3361 tccttgaggg atacgtagat gggctcctgg tcgaccacca cgaagctgcc cgcggcgatg
     3421 tgcaccctgg ggttgtgcgg gtcgggcctg ccgaaatgcg tcactcgttc gtgggcgcat
     3481 gccgtcaatc cgaaaatcag cgcaagtccg atcagggcat tttttctcgt cttcatgggc
     3541 ttcctccact tgtgatccga tcgggatctc gcgaatgctg cacctgcggc cccgcgcccg
     3601 caagacgggg ccgcggatcg ccttaggcca cctgtcatcc cccccccacc ccctcccgcg
     3661 ttagggggtc gcggccaatt ccggccgccc gccctggagg cacgccgtgt tcgaatcgct
     3721 ccaatccgct ctggccacca ataagttcgt ctccggcggc atcgtgctgg ccgcgctggg
     3781 cgtggctgcg atgtggcttc gcgaggtgcc ggtcaaggtg gcccggtggg cgaaacattt
     3841 cttcgtgacc acgctctcga tcgacagccg cgaggaactc atgtttcccg cgctcgtcga
     3901 gtacatggac acccgcgagg ccctgcgccg gctcaacaac ttcaccgtcc gcacggtgcg
     3961 ccagcaggat tcgagctacc agagcctgca cgacgaactg cagcagggcg gccggccgca
     4021 gacgctcttc tcccccggcg agggcttcca tctcttcgtc ctggacgggc gcctcatgtg
     4081 gatgaagcgc gaggtgcagg tgggcgtctc gatcgtcgag aaaatcacgc tctccacgct
     4141 gggccgcgac aaacgcccgc tggaggcgct cgtgcatgcg gcgatggagc atcgcatcga
     4201 gcgggagctg aaccgcatcg ccatccatgt tccgagcacc tacggcaacg actggacgcg
     4261 cgcgcgcctg ggcaacaatc gcaagctcca gtcggtggtc ctcaaggcgg ggcagaagga
     4321 ggcgatcctc gcggacctgg agcgcttctt cgcgagccgg gagcgcttct tcgcgagccg
     4381 ggagcgctac gagacgctgg gcatcccgtg gcgccgcggc tatctcctct acggcccgcc
     4441 cggcacaggc aagacctcgc tggtcacagc gctcgcttcc gaactttcgc tcaacgtgtg
     4501 cgtgctgagc cttgcctcgc ccaacatcac cgacgagaag gtcggcaacc tgctggccag
     4561 cgtgccgcgc cgttcggtga tcctcatcga ggatgtcgac gccttcttcc agaatcgcag
     4621 caaggccgat gcgcaggtgc gcgtgtccta ctccggcttc atcaacgcgc tggacggtgt
     4681 cacggcccac gaagggtcgg tggtgttcct caccaccaac catccgcagc tgatcgacga
     4741 ggcggccatt cgttccggtc gcgtggactt ccgtctggaa ctggggctat gcgatcgcga
     4801 ccagctcgaa cgcatgttca cgaagttcat ccacgacccg gtggccgcgg cccggttcgc
     4861 ggacgcggtg caggccgatc gctggtcgcc ggcgcaagtg caggagcggc tgttgaaagc
     4921 cggctccgtg gacgaggcgc tggagttgtt ccgcgaacgg gcggaaggcg aggtcgcgat
     4981 gctgcgggcg gcctgaacgg aaaggtctcc cgcgcgcggg ttaccagcgc gaccggggcc
     5041 gcggcggcgc tcgccgccgg ccggaccagg gcgattcgtg aaggtcggga gtcggcggag
     5101 caactccttc cgcgcgaggc atcattaccg tgtcgatcac atggatcacg ccgttcctgg
     5161 aggcgatgtt gggcgtcgtg acgttcgcgt ggtccacccg catcccttcg tcggtggcac
     5221 cgatggtcag catgccgccc tcgacggtct tcgcggtgcc gttcggaatg tccgaggtct
     5281 tcatggtgcc gcgcagcgtg tggtagttga tgagtgcggc caggcgcttc ttgtcccgca
     5341 tcagcgcatc gagcttttcc ccgggaagct tgaggaatgc ctcatcgctg ggcgcgaaga
     5401 gggtgaacgg cccggggccc tggaactgct ttaccagacc cgcggccgcc atcgcgctgg
     5461 agagcgtggt gagggtccgg ttggcaaggg tcgtttcgac gatgtccttg ggcggcaatt
     5521 tcatgggaat tccttcgcgg agatcggcaa gtctccacta tgggcgcatg ggccgggatt
     5581 ccccggagac accggaaata attgcattga cttcccgggc aggcgcgaga ctcgggcgca
     5641 ggggcgccac gggatgcagg tgcgcgggcg cggaacgcaa ccgggggcaa gggcatcgcg
     5701 cagatgcgcg ccgactacct tcgtcggcgc gccggcgatg ccctcgagcc ggcaatcagg
     5761 cgccggtacg cggcgccgtg ccgtgaatcc tcaccttcgt gcgcacgttc gcgacctccg
     5821 actcccgttc catttcgagc tggagggcgc cgccgttttc gggatgcgct gaagccgtgt
     5881 aggagccgtt cagcgaggcg ctcgagcagc ccgttgccgt gatcgtgccc gtatgcaact
     5941 gcagcgccgc atccgtgagc gtgagcgtcc cgttgaatga gcaggcgaag ccagtgcccg
     6001 agacgccacc gccggtcgtc acgttgaact gcgattcgct cgaaagcgac gaggtcgtcg
     6061 tgttgtcgct gccgttggag cggtcacgga tctcgatgtc ggcggtgaag gcgctcttgt
     6121 aggtgcccgc gatcccggcc gggggcgacg gcggattgac aggcgtcgtg ggcaggacgg
     6181 cagtgctcac gagcttgcct tccacctgaa ccttggtcgt cacgccggcc gtctcgcttt
     6241 ccttctcgaa ttccacctcg agcatcgcgt cgtcgcgctt caccttcacg gtggcgtagg
     6301 tgccgttgaa gacgccttcc gtgcagccgc tcgcggagac gctgccgccg aatatgccgg
     6361 ccaccgaagt ggtcgaaagc gttcccgaga agctgcagcc gaatcccgaa ccggcaatgg
     6421 cgccgccgct tgaaatcgcg agttgcagcg ggcgattcga ttcgacgagg aaggtctccg
     6481 tgccgccctg gcgctgcagc gccgtccagc ccaccgtgcc agcccagtcg ccggtaatgc
     6541 cgccggcgga agggttcgtg gagggcggcg gggtgaccgg tggcgcgccg gtcgtgagca
     6601 ctgcctcgac cgatgcctcg ttcgcgttgt cgccgcgttt catgtgcacg gcgagcgtgc
     6661 cgttgccgaa gcgctcgagc tggaagcggc tgtaggcccc gctgaaggcc gcgtcggtgc
     6721 agcccgaagc ggagatcgtg ccggagaaga gcgaacggaa tccgtcgccg gactggacct
     6781 gcccggtgaa ggcgcagccc tggccgctgc cgtccacctt gccgtcggcg gcgatggtga
     6841 gcgcaagggg cgcagcgacg cgcgtttcga cggcacccgc ctcctttttc cacctggccg
     6901 tgccggtcca tgcggccgcc agcgggtcga agttggtcgt ggtggggccg aggttcgcat
     6961 cgctgccgtt cggcttggca cgcgcgcaaa tcgccacgcc ttcggcaacc cacgagcggt
     7021 cgaccatcag gtcgcgcagc tgcgagtcgt cgacgaagcg atggttggac ccttcgccgg
     7081 aagtgaagcc gttgttgtag acgcgcgaaa tcgccagggt gccagccggg caggagccgc
     7141 tcgccggcat ctcgatgcgg aagccgattc cctcgtacct ccagcccagg atgggcgcgc
     7201 cggtcgcctg ggcgctgctg cgctccgcgg cttcgaggct cttcaggctt gcgcattcgg
     7261 cggcgtcccc ggtgtagaag tgcgagttgg cgccggtcga atagaagcgg cacacctcgg
     7321 aggccgtgga cggcgccttg gcaggatcga gccatgcctg gaaggaccgg ccggtgcgca
     7381 cccagcccgg gcccgcgccg ccgtcgtcga tgatcctcgc ctccgaagcg gtggcggtga
     7441 tgaaatagtg gttcgtcgtg aggttgtagt actccacagc ttccgtttcc ggttctgcgg
     7501 actgcacggc gaaaggcagg gccgccaggg cggcaagggc gagagtcagc tttttcaggt
     7561 tcatggatca ctcctagaca acatggggac acggggtccg gtatctaacc agcagcacac
     7621 ttcgtgccag cccaattcat gatcccaact cattgatcgg taacgataac gagactggca
     7681 tcccgaaggc ccgaccgcgc ttgtctgccg ggagcgacga cagtgggcag atacccaaga
     7741 cgtttcttcg cggcgcccgg ccagggcctt gccgaagcga aagcccatag ggcggcatgc
     7801 aaagcccgca cttcacggag cccactgtag tcaatccgga aaggggccga atgtggtcat
     7861 ttgccgcagg cccttcgccc ggacccgcgc ccgccagccc tttgcgccag cctcactcga
     7921 ccacggtctg cgactgctcc cgcaggtact gggtgacgta gtagaaggac ccgatcgcct
     7981 tgccggtgga tcccgaaccc ttccagcccc cgaagggctg gtagcccggc caggcgcccg
     8041 tggtggcgcc ctgcgggcgg ttcacgtaga cgacgccggc ctcgatccgc tcgaggaagg
     8101 tgtcgatctc gcccggcgcc ccgtagaagc cggccgtgag cccgtaatcg gtgtcgttgg
     8161 cgcgcgtgag cgcttcgtcg agcgaatcga ccgcgcccac cagcacgaag ggcaggaagt
     8221 gctcgtcgcg ccagagacgc gaagcccacg gcgcggtggc cacggtgggc gcgacgaagt
     8281 agccgcgcgc gaggtccccg tcggcgagtt cgcgtccgcc ggcgtacacc cgaccgccat
     8341 cggcgacttc cgcgacgacc ttgcggtagc gctcgcgggc cgccgcgttg atcaccggcc
     8401 ccagccaatt gcgccgcaag gtgggatcgc ccaccgcggc ctcgcccgcc agctgggcaa
     8461 gccgggcgac cagttcatcc atgaccggac gttccgccag cacgcgcgag gccgcggagc
     8521 atttctgccc ctgcaggccg aaggcggagc ggaaaatgcc ggtggcggcg cgacccagat
     8581 cggcgtggcg tgtgacgacg actggattct tgccgcccat ctcgacaatg cacgggcgcg
     8641 gccagcgccc ctcgccgaac aggcgcacga tgcgcatccc cacctcgccg gagccggtga
     8701 acgtgatgcc ggcgacgtcc gggtgccgca ccaacgcctc tccgatttcc gcgccgctgc
     8761 ctgtcacgta attcaggacg ccgtccggga tcccggcctc gcggaacacg tccatcagga
     8821 gcgccccgca ccaggccgtg tcgctcgcca ccttgaagac gaccgtattg cctgccacga
     8881 gcgccgcgcc gatcgggccg ccggccagcg ccatggggaa attgaacggc gcgatcacga
     8941 cccacacgcc atgcggcctg agcaccgtgc ggttgcggct ggtgaagccc gcgatggggt
     9001 cgtcgggcag gtaacgcagg aagccgccgt tctcttccat ctccgcgcaa taccaggaga
     9061 tgaggtcggc ggtctcctgc acctcgccga tcgcttccat gcggttcttg cccacttcca
     9121 gcgccatggc ggcgccgagc tcgtagacgc gctcctcgat ccgcttcgcg accctgcgca
     9181 acagcgcaac acgctcgggc caggccgtcc tggcccacgg ccggaaggcc gcgcgcgcgg
     9241 cggcgaccgc atcggccgcg tcctgcgccg tgccgcgggg caagcgcgcc agcaaccagt
     9301 cgcggtcgat gggggagcgc gattcgaatt ccgcccgtcc ggccctcgcc tcgccgccga
     9361 tgcgcatcgg acaaggcgcg cccagcctcc ccttgacctc ggcaagcgtc gcctcgaagc
     9421 gctggtgcag cgcctcgggc gggtcgaaca tcgtggaata ggtgagcttg aaaggctggt
     9481 cctgcacggc tcgctcccgc ggaggcggct tcatgttcgc gcgacgaact cggggtcgcc
     9541 ctgctgctcg cgctcgaact tgaggaagtc gtagtacccg cagcgcctgc tgtccgggta
     9601 attgcggcag acgccggggc gcgcctcgta caccgtgcag cggcgcagct tctggtcgag
     9661 gaacgtgcag gcggagtcga acaccttgtc gctgcggtgg cgcagcaagc gcaccttctc
     9721 cttgggatcg agcgtggtga agcgcgcctc ggccgtttcg tagtcgagcc cgaaatggcg
     9781 ggcgagccgc gcaatgtcgc gcttgccgac ctcgatgtcc gagtaggtgc agcaataggc
     9841 ggggcacttc gcgcaggagt agagcacgcg gacggggacg ggaaagggct tggtcttctt
     9901 catcgatcgc aggggcgtgg caaaagggcg caattgtacg cgaccccgaa ggcgcccgga
     9961 cgatgacggc gccgggtcgc atcgcccatc gtgcatcgac gaattcccgc gtgcaagcaa
    10021 cgcgagtcgc acggccccgt gccgcatccc cacttcttga ttccggtcaa cgctgcgcta
    10081 ccattccgta atagcctgaa agtccgggga cccgctgcgg aagcgaaaag gggtttcatg
    10141 cgcgatatgc acgat
//
LOCUS       DASUEV010000094         5120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1617189, whole genome shotgun sequence.
ACCESSION   DASUEV010000094 DASUEV010000000
VERSION     DASUEV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617189"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1963
                     /gene="alaS"
                     /locus_tag="VFV55_02980"
     CDS             <1..1963
                     /gene="alaS"
                     /locus_tag="VFV55_02980"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX4943288.1"
                     /translation="GVMHPLPKPSVDTGMGLERLTAVLQHVHSNYEIDLFQTLVRAAA
                     RETGTRDLASPSLRVIADHIRACAFLICDGVIPGNEGRGYVLRRIARRAMRHGYKLGQ
                     KQPFFYRLVADLAAEMGDAYPELREAEGRVTATLRQEEERFGETLENGMAMLDAALAG
                     GKRQLDGDTAFRLYDTFGFPVDLTADIGRERGFTVDMAAFDAAMTAQQERSRAASKFK
                     AGAQLEYGGIKTSFRGYDTLSEEGRVVALYREGSPVGSLSAGESGIVVLDRTPFYAES
                     GGQVGDRGELTKGGVCLTLFAVEDTQKIQPEVFGHYGVVKTGELAVGDTVAAEVDLDK
                     RARTMRHHSATHLMHKALREVLGAHVQQKGSLVDEDKTRFDFSHNAPMTDAEIRRVEE
                     IVNAEILANAATRSRVMPIDEAQKLGAMMLFGEKYGDEVRVLDIGTSRELCGGTHVAR
                     TGDIGYFRVVSEGGVAAGIRRVEAIAGSVALAQAQADRDRLARAAEALKAPPQEIDAK
                     IAQVVENVKALEKEVAKLKGQLAAGKLDDVLASGMRTIKGVKAVAVLLDGADAGMLRD
                     YLDKVKERLGSGVALLASVADGKVSLIAGVTKDLTGRIKAGELVNFVAQQVGGKGGGR
                     PDMAQAGGTDPAALPKALQSVDGWLGERL"
     gene            1996..3294
                     /locus_tag="VFV55_02985"
     CDS             1996..3294
                     /locus_tag="VFV55_02985"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEX4943289.1"
                     /translation="MGHPNFILPEDDLRSALNDEVHSRPPVRLAGAQHIVYLAVRHDP
                     AERAAHRQALATIAQSAGAAVAPQVDHAVVTLAEGLRFKWEGHAEFSSYTFFAEGAGE
                     RVPAAVLESAGWPEIAATLPGEVLVAVSIDLVPFQGDSPPTNAFHTQSDTIVGGAVAE
                     GRGWGYTDFRIREDGFTRFLLLNREMGLGQAGRMVQRLLEIETYRMMALLAFPVARKA
                     APDLGAMETELAAIVGRIAGATPQDEARLLDDLTRLAARVEHLLSATRFRFEAADAYD
                     ALVSRRLAELREVRIPGLSTIEEFLHRRLAPAMATCRSASRRIGNLASRVTRASALLR
                     TRVDIAREQQNQKVLEAMNRRGRVQLRLQQTVEGLSVVVITYYGVSLVGVLAKAAKAA
                     GVDWNPEVVMGIAVPFVAALVFGGVRRVRKALEGEEKGGA"
     gene            3291..3833
                     /locus_tag="VFV55_02990"
     CDS             3291..3833
                     /locus_tag="VFV55_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4943290.1"
                     /translation="MSAEPFTFCPRCAGPLAERLVADEDGRGVLRRACTDACGFVQWG
                     NPVPAVGALVEHEGEIILARNRAWPEGWFALVTGYLEAREDPKAAVVREVKEELGLDV
                     VETNLIGNSIFERKNEIMLCYHAVATGTVQLGAELAEYRRHAPATLKPWPRATGFAVA
                     DWMRARGLPFEFVDFAAARR"
     gene            3830..4552
                     /locus_tag="VFV55_02995"
     CDS             3830..4552
                     /locus_tag="VFV55_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4943291.1"
                     /translation="MSARPDMAPEGVARIAGYLRAAGLAQVEGHEDEVALAIGDLLEA
                     MGEKVAIDDPGSLYTYPVPMLTADGACSVTDELAPVPYDLTGILGGRGEQATRRLALL
                     ERLVERAQETTGSDWVGIYQRRVNPAGHPVLVKLSYVGRPSRAEFPLNREFAAGSTNS
                     TVGLTGRALVIDDVKKHVQAGGGFYVCDGDVQSEACLPLFDDARNVTGIVDAEAIPTS
                     FYGPARLATLVALAIVASAVLP"
     gene            4557..4964
                     /locus_tag="VFV55_03000"
     CDS             4557..4964
                     /locus_tag="VFV55_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4943292.1"
                     /translation="MINPTGIDHIVLRVRDADAMVAFYIGVLGCTLERRRDDIGLVQL
                     RAGRSLIDLVPVDGKLGRQGGAAPGPEGRNLDHLCLRIDPFDGEAIRAFLAARGCEPG
                     EIAMRFGAEGEGPSIYVRDPEGNTVELKGPVGK"
BASE COUNT          795 a         1755 c         1837 g          733 t
ORIGIN      
        1 cggcgtcatg catccgctgc ccaagccctc ggtggacacg ggcatgggcc tggagcgctt
       61 gacggcggtg ctgcagcacg tccactcgaa ctacgagatc gacctgttcc agacgctggt
      121 aagggccgcg gcgcgcgaga cggggacgcg cgacctcgcc tcgccgtcgc ttcgcgtcat
      181 cgccgaccac atccgtgcct gcgccttcct catctgcgac ggcgtcattc ccggcaacga
      241 gggcagggga tacgtgctgc gccgcatcgc ccgccgcgcc atgcgccatg gctacaagct
      301 cgggcagaag cagccgttct tctaccggct ggttgccgat ctcgcggcgg agatgggcga
      361 cgcttacccg gaactgcgcg aggcggaggg ccgggtgacg gccacgctgc gccaggaaga
      421 ggagcgtttc ggcgagacgc tcgagaacgg catggcgatg ctcgatgccg cgcttgccgg
      481 cgggaagagg caactggacg gcgataccgc cttcaggctc tacgacacct tcggctttcc
      541 ggtggacctc acggcggaca tcggccgcga gcgcggcttc accgtggaca tggcggcatt
      601 cgatgcggcc atgacggcgc agcaggagcg ttcgcgcgcc gccagcaagt tcaaggccgg
      661 agcgcagctc gaatacggtg gcatcaagac atccttccgc ggctacgaca cgctttccga
      721 ggaaggccgg gtggtcgcgc tctatcgcga ggggagcccg gtcgggagcc tctcggcggg
      781 agagtccggc atcgtggtcc tcgaccgcac gccgttctat gccgagtccg gcggccaggt
      841 gggcgaccgc ggcgagctca ccaagggcgg cgtatgcctc acgctctttg cggtggaaga
      901 cacgcagaag atccagcccg aggtattcgg gcactacggc gtggtgaaga ccggcgagct
      961 cgccgtgggg gacaccgtcg cggccgaggt ggatctcgac aagcgcgcgc gcacgatgcg
     1021 ccaccactcc gccacgcacc tcatgcacaa ggcgctgcgc gaggtgctcg gcgcgcacgt
     1081 gcagcagaag gggtcgctgg tcgacgagga caagacgcgc ttcgacttca gccacaacgc
     1141 gccgatgacc gacgccgaaa tccgccgcgt ggaggagatc gtcaacgccg agatcctcgc
     1201 caacgccgcc acgcgctcgc gcgtcatgcc catcgacgag gcgcagaagc tcggcgcgat
     1261 gatgctcttc ggcgagaagt acggcgacga ggtgcgcgtg ctcgacatcg gcacctcgcg
     1321 cgagctgtgc gggggcaccc atgtcgcgcg caccggcgac atcggctact tccgcgtcgt
     1381 ctcggaaggc ggcgtcgcgg cgggcatccg tcgcgtggag gccatcgccg gcagcgtcgc
     1441 gctagcgcag gcgcaggccg accgcgaccg cctcgcgcgc gcggccgagg ccctcaaggc
     1501 cccgccgcag gagatcgacg cgaagatcgc gcaggtcgtc gagaacgtga aggcgctcga
     1561 gaaggaagtc gcgaaactga agggccagct cgcggccggc aagctcgacg acgtgctcgc
     1621 ctccggcatg cgcacgataa agggcgtgaa ggccgttgcg gtgctgctcg acggcgccga
     1681 tgccgggatg ctgcgcgatt acctggacaa ggtgaaggag cgcctcggca gtggcgtggc
     1741 gctcctggct tcggtggcgg acggcaaggt gtccctcatc gcgggcgtca ccaaggacct
     1801 gacgggcagg atcaaggcgg gcgagctggt gaacttcgtg gcccagcagg tgggtggcaa
     1861 gggcggcggc aggcccgaca tggcccaggc tggcggcacc gaccccgccg cgctgccgaa
     1921 agcgctccag agcgtggatg gctggctggg cgagcggctc tagctagcct gcgcgccgac
     1981 cggtagcgca agcccatggg ccatcccaat ttcatcctgc cggaggacga ccttcgctcc
     2041 gccctgaacg acgaggtgca ttcgcggccg ccggtgcgcc tggccggtgc ccagcacatc
     2101 gtctacctcg cggttcgcca cgatcccgcg gagcgcgccg cgcaccggca ggcgctcgcc
     2161 acgatcgcgc agtccgcggg cgcagcggtt gcgccccagg tggaccacgc ggtggtgacg
     2221 ctggccgagg gactgcgctt caagtgggaa ggacacgcgg aattctcgag ctacaccttc
     2281 ttcgcggaag gcgcggggga gcgcgtgccc gccgcggtgc tcgaaagcgc cggctggccg
     2341 gagatcgcgg cgacgcttcc cggtgaagtg ctggtggcgg tctcgatcga cctcgttccc
     2401 ttccagggag attcgccccc caccaacgcg ttccacacgc agtcggacac gatcgtgggc
     2461 ggcgcggtgg ccgagggtcg cggctggggc tacaccgact ttcgcatccg cgaggacggc
     2521 ttcacgcgct tcctcctgct caaccgcgag atgggactgg ggcaggccgg gcgcatggtg
     2581 cagaggctcc tcgagatcga gacctaccgc atgatggcgc tgctcgcttt tccggtggca
     2641 cgcaaggccg cgccggacct gggcgccatg gagacggaac tcgcggccat cgtgggacgc
     2701 atcgccggcg ccacgccgca ggacgaggcc cggctgctcg acgacctcac gcgactggcc
     2761 gcgcgcgtgg agcacctgct gagcgccacc cgcttccgct tcgaggcggc cgacgcctac
     2821 gatgcgctcg tctcgcgccg cctcgcggaa cttcgcgagg tacgcatccc gggcctgtcc
     2881 acgatcgagg agttcctgca ccgccgcctc gcgcccgcga tggccacctg ccgcagcgct
     2941 tcgcgacgca tcggcaatct cgcgtcgcgc gtgacgcggg caagcgcgct gctgcgcacg
     3001 cgcgtggaca tcgcgcgcga gcagcagaac cagaaggtcc tcgaggccat gaaccgccgc
     3061 ggacgcgtcc agctgcgcct gcagcagacg gtggaaggcc tgtcggtcgt cgtcattacc
     3121 tactacgggg tctcgctcgt gggcgtgctg gccaaggccg cgaaggccgc gggcgtggac
     3181 tggaacccgg aggtcgtcat gggcatcgcg gtgcctttcg tggcggcgct ggtgttcggc
     3241 ggcgtgcgcc gcgtgcgcaa ggccctcgag ggcgaggaaa agggcggggc atgagcgccg
     3301 agcccttcac gttctgcccg cgctgtgccg ggccgctggc cgagcgcctc gttgccgacg
     3361 aggacggccg gggcgtcctg cgccgcgcct gcaccgacgc ctgcggattc gtccagtggg
     3421 gaaaccccgt gcccgccgtt ggcgccctgg tcgaacatga aggcgagatc atcctcgccc
     3481 gcaaccgcgc gtggcccgag ggctggttcg cgctggtcac cggttacctc gaggcccgcg
     3541 aggacccgaa ggcggcggtg gtgcgcgagg tgaaggagga actcggcctc gacgtggtgg
     3601 agaccaacct catcggaaac tccatcttcg agcgcaagaa cgagatcatg ctgtgctatc
     3661 acgcggtggc caccggaacg gtgcagctgg gcgcggaact cgccgaatac cgccgccacg
     3721 cgccggcgac cctcaagcca tggccgcgcg cgaccggatt cgcggtcgcg gactggatgc
     3781 gcgcccgcgg cctgcccttc gaattcgtcg acttcgcggc ggcccgccga tgagcgcccg
     3841 gccggacatg gcgcccgaag gggtggcgcg gatcgcgggc tacctgcgcg ccgcggggct
     3901 cgcgcaggtg gagggccacg aggacgaagt cgccctggcc atcggcgacc tcctcgaggc
     3961 catgggcgag aaggttgcga tcgacgatcc gggctcgctc tacacgtacc cggtcccgat
     4021 gctcaccgcg gacggcgcgt gttccgtgac cgacgagctt gcccccgtgc cctacgacct
     4081 caccggtatc ctcggcggac gcggcgagca ggccacgcgg cgcctcgccc tcctcgagcg
     4141 gctcgtggaa cgcgcgcagg aaacgaccgg ctccgactgg gtcggcatct accagcggcg
     4201 cgtcaacccg gcggggcatc cggtgctcgt gaagctctcc tatgtcggac gaccgagccg
     4261 cgccgagttt ccgctcaacc gggagttcgc cgcgggcagc acgaacagca cggtcggcct
     4321 caccgggcgc gcgctcgtca tcgacgacgt gaagaagcac gtgcaggccg gtggcgggtt
     4381 ctacgtgtgc gacggcgatg tgcagtcgga agcgtgcctg ccactcttcg acgacgcgcg
     4441 caacgtgacc ggaatcgtcg acgccgaggc gattccgacg tcgttctatg ggcccgcccg
     4501 cctcgcgacc cttgtggcgc tcgccatcgt tgcctccgcg gtgctgccct gatcccgtga
     4561 tcaacccgac cggcatcgat cacatcgtcc ttcgcgtgcg cgacgccgac gcgatggtcg
     4621 cgttctatat cggcgtcctc ggctgcacgc tggagcgccg ccgcgacgac atcgggctgg
     4681 tgcagcttcg cgccggccgt tcgctgatcg acctggttcc cgtcgacggc aagctcggcc
     4741 ggcagggagg cgcggcgccg ggcccggaag ggcgcaacct cgaccacctc tgcctgcgca
     4801 tcgatccgtt cgacggcgag gcgatccgcg ccttccttgc ggcgcgcggc tgcgagcccg
     4861 gcgagattgc aatgcgcttc ggcgcggagg gcgaaggccc gtcgatctac gtgcgcgatc
     4921 ccgagggcaa cacggtcgag ctgaagggac cggtcgggaa gtgagggcgc gagagcgagc
     4981 gtgaccggcg aatttggagc taggccgggg atggggcaag tctatcggcg tcctgggcac
     5041 gcaagcggga ccgtcgaatg ggtcccaaaa agtcggggcc agccaaacac gtctgtcccc
     5101 gactttttac ccattgagag
//
LOCUS       DASUEV010000095         7803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_141861, whole genome shotgun sequence.
ACCESSION   DASUEV010000095 DASUEV010000000
VERSION     DASUEV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141861"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..571
                     /locus_tag="VFV55_03005"
     CDS             101..571
                     /locus_tag="VFV55_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX4943293.1"
                     /translation="MSYWLMKSEPAEFSIDDLARAPGKSTPWFGVRNYQSRNYMRDAM
                     RVGDGVLFYHSSCEVPGIAGLARVSSAPHPDETQFDRKSPYFDPKAARDNPRWILVDV
                     ELVKKTRLLSLEELRGYAELADMVVLRRGNRLSITPVTPGEWAIVTKILARDAR"
     gene            561..1364
                     /locus_tag="VFV55_03010"
     CDS             561..1364
                     /locus_tag="VFV55_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4943294.1"
                     /translation="MPGEALTWLTYAGLGVVVGFFSGLLGIGGGSMIVPILGLVFVAF
                     GFPPDQVMHLSLGTSLAAILAATASGARAHQAHGAVRGDIVKGLAPGIAAAGLAAGAI
                     ARVVSVAFLKYFFLAFMFYVTTQILFGLKPARARPVPGRLGLFGVGALIGGLSGLAGV
                     GGAMISVPFMTAWGVPFKSAIGTSAAISFVVAVAGTIGYVAAGLTVPSLPAWTVGYVY
                     LPALLGISVTSVFVAPWGARVAHRLPVGHLKKAFAVFLLALAVKLITAL"
     gene            1436..2812
                     /gene="trkA"
                     /locus_tag="VFV55_03015"
     CDS             1436..2812
                     /gene="trkA"
                     /locus_tag="VFV55_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX4943295.1"
                     /translation="MKILILGAGQVGASVAESLVSEKNDVTVVDTSAERLKDLQGQFD
                     LRTVTGNAAHPEVLAEAGAADTDMLFAVTASDETNLVASKVGHAVFNIPTRIARIRST
                     WFERHPELLEGDCFSVSHAICPEQIVTEYILRLIEFPEALEVVEFAGGKVDLVAVRAV
                     VEGTLVRHPLRDLEELLPDIGAKVAAIYRNDRPLALSPDTVVHPGDEVFILADARDIR
                     EVMTELRKMDRPVRRVIIAGGGNIGLRLARAIEGRMNVKVIEASKRRCEFLAGQLASA
                     LVLNGDVSNEDLLGDENVDETDLFVSVTNDDEANIMSALLAKRMGARRVIALINRRAY
                     GDLMQGGQIDIAISPAQATLGSLLEHVRRGDVVAVHSVRRGRAEALELIAHGDRKTSR
                     VVGRRIREIDLPPGATIVGIIRGDRVIVAKDDGLVIEPDDHVLVFLTQTRMIPKVEKL
                     FEVSVGFF"
     gene            2815..4338
                     /locus_tag="VFV55_03020"
     CDS             2815..4338
                     /locus_tag="VFV55_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX4943296.1"
                     /translation="MSRSIPQERRAQEGGRRPVDAVLRHFPVLHVLSAAVLLFSPAFL
                     VPLVFSMALADGAHAAFETGFLVTFVTGLFTFFLTRRRHRRELQPRDGFLLVSLVWSV
                     VPAFATIPLLLAMPGLSFTDAYFEAVSGMTTSGATVLAGLDALEPSVNIWRSLLVWIG
                     GMGIIVLAVAIMPLLGVGGSQLFKAESPGCMKDTKLTPRIGDTAKGLWTVYAAVTAAC
                     LVSFRLAGMTWVDAVIHAFSTMGLGGFSSHDDSFGYFKSPAIEAVTTFFMLAASLNFA
                     THFLAVRRRSPAPYAADPEVRRVLIWMVGSSVGIAAFLVLRGTYPDFATALRHASFNV
                     VSIASTTGFASLDYAQWPAFAPIWMIFLSCFATSSGSTGGGIKMVRAVVLFKQTMREM
                     TRIIHPKAVLPVKFGEQVIENNVIFAVLAFMLMWGGTIIVMSMLLLASGLDVVSAVTA
                     VLACINNMGPGLGAVGPSSNYAGLTDFQTWVCTATMLLGRLELFTMLVLFTPAFWRK"
     gene            complement(4313..5608)
                     /locus_tag="VFV55_03025"
     CDS             complement(4313..5608)
                     /locus_tag="VFV55_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX4943297.1"
                     /translation="MTSSGPVDAQPAWNRAVAGVLVAFLFVLPFSSNAALRNALLGFA
                     AACVLYGSAKGMVARPRLPSWRVLAPILAYGAWSIASVAWSVEPAYSLSELRPGLLYP
                     FIAFLAFFAATPSVAAIDRWAWSLSAGLAALGLVAGAELVLDGWWDPQRWHSDAGYYA
                     THVVLALPLLAWAFLRAGTAFRGARVVLAAVAVLTLVVTSWNDNRIAWVALAAMTLLA
                     VFLSRASLAGPLRSQVVAFTAAALIAFSALFVLSIQQRTTKLEHTEQAAEAQLSRDPR
                     LAIWDHAARLNGEAPWIGHGYGRGILRQQFRNGVSPGVDNPLYTHAHNTPINVLLQGG
                     AIGLGLFAWMVAALVREMAAGLKAGPPRRCAATLGLVLVAGFAIRNMTDDFLVRHSAL
                     LAWSLAGAILGALRPAPAPVVSGTASDTAGTATSARTPA"
     gene            complement(5625..6758)
                     /locus_tag="VFV55_03030"
     CDS             complement(5625..6758)
                     /locus_tag="VFV55_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX4943298.1"
                     /translation="MTNTRIDPRALTRELLAFNTINPPGMERACARHLGAILEAAGFR
                     TAYHEFAEARTSLVAEIGGDPVRPPICFTGHIDTVPLGAKAWSKDAFAGETDGDRLYG
                     RGSTDMKSGIAAFVAAAVELAPLLARSPGVVLVITASEEIGCEGSKFLADAKLLDRAG
                     AIVVAEPTANYPYVGHKGLVWFEIETTGVTAHGSMPELGDNAINRMARVIGDLEQFRF
                     PVESHPVMGNPTLSVGTIRGGLNTNSVPDSAKITVDIRTVPGIDHAHLCHSLEHLLGP
                     RGATVKPFVDTPSLYTEPANEWVQEVFEVCSPFLEGRPTPKTITFSTDGAYLKQGYGG
                     PPAVVLGPGEPRLAHQTDEWCSMSRIEQSVDTFATLMRRWCGV"
     gene            complement(6772..7518)
                     /locus_tag="VFV55_03035"
     CDS             complement(6772..7518)
                     /locus_tag="VFV55_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4943299.1"
                     /translation="MEFFTFAWITDPQIWLSLVTLCALEIVLGIDNLVFIAILTGRLP
                     EHQRSLGRKVGLALALVTRLMLLATLAWIVGLTQPVFTIAGKDFSWRDIILIAGGLFL
                     LYKATVEIHEMIEKEEGDPDKAEGAAGVTFKGVVIQIAIIDIIFSLDSVITAVGMADH
                     LWVMVVAVVMAMLIMIAASDPLANFVSAHPTVKMLALSFLLLIGVVLIADGLGVHVPK
                     GYIYFALAFSVAVETLNQWARRRRKRLKGA"
     gene            7548..>7803
                     /locus_tag="VFV55_03040"
     CDS             7548..>7803
                     /locus_tag="VFV55_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943300.1"
                     /translation="MSHARCRTPASSRESVHDPQGQLRRTQQKLYDYINRLQKGIGAA
                     GQATTYGYAGDNSYWTVTDPLFRKTTNYKDVFDRLNYVTDP"
BASE COUNT         1226 a         2636 c         2687 g         1254 t
ORIGIN      
        1 ggtcaggtta ctttacacat cacccggcga agccgggatt cgatggactg taaagtaacc
       61 tgacccctta tcgaaaagta acctgacccc ttttcactca atgtcctact ggctcatgaa
      121 gtcggagccg gccgagttct cgatcgacga tctcgcccgc gcccccggca agtccacccc
      181 ctggttcggc gtgcgcaatt accagtcgcg caattacatg cgcgacgcga tgcgcgtggg
      241 cgacggcgtg ctcttctacc actcgagctg cgaggtgccg ggaatcgcgg gcctcgcgcg
      301 cgtgtcgagc gcgccgcacc ccgacgagac gcagttcgac cggaagagcc cgtatttcga
      361 tcccaaggcc gcgcgcgaca acccgcgctg gatcctggtg gacgtcgagc tggtgaagaa
      421 aacgcgcttg ctgtcgctgg aggaacttcg cgggtacgcc gaacttgcgg acatggtggt
      481 tctgcgccgc ggcaatcgtc tttcgatcac gccggtcacc cccggcgaat gggcaatcgt
      541 caccaaaatc ctcgcgcgcg atgcccggtg aagcgctcac ctggctgacc tacgccggcc
      601 tcggcgtcgt cgtcgggttc ttctcggggc tgctcggcat cggcggcggc tcgatgatcg
      661 tgccgatcct ggggctcgtg ttcgtcgcgt tcggcttccc gcccgaccag gtgatgcacc
      721 tgtcgctcgg cacttcgctc gcggcgatcc tcgccgcgac cgcctccggc gcgcgcgcgc
      781 accaggcgca cggcgcggtg cgcggcgaca tcgtgaaggg gctggcaccc ggcatcgcgg
      841 cggcggggct cgcggcaggc gcgatcgcgc gcgtggtgtc cgtggctttc ctgaagtatt
      901 tcttcctcgc cttcatgttc tacgtcacca cccagatcct gttcgggctg aagcccgcgc
      961 gcgcgcgtcc cgttcccggt cgcctgggac ttttcggcgt gggcgcgctc atcggcggcc
     1021 tgtcgggcct cgcgggcgta ggcggcgcga tgatctccgt gccgttcatg acggcgtggg
     1081 gcgtgccctt caagtccgcg atcggcacgt cggccgcgat cagcttcgtg gtggccgtcg
     1141 cggggaccat cggctacgtg gcggcgggcc tcacggttcc gtcgcttccc gcgtggacgg
     1201 tgggctacgt ttacctgccg gcgctcctcg gcatctccgt gacgagcgtc ttcgtggcgc
     1261 catggggcgc gcgggtggcg caccgcctgc cggtgggcca cctgaagaag gcgttcgccg
     1321 tcttcctgct cgccctcgcc gtgaagctga tcacggcact atgagcgccg cggcagcgcg
     1381 ccgggcgcac ccggcgtaag attcgcgcag tcagcccgac aatttccgct cccccttgaa
     1441 aatactcatc ctcggcgccg gccaggtcgg cgccagcgtg gccgaaagcc tggtctcgga
     1501 aaagaacgac gtcacggtcg tcgataccag tgccgagcgc ctgaaggacc tgcagggcca
     1561 gttcgacctg cgcacggtca ccggcaacgc ggcgcacccg gaggtcctcg ccgaggccgg
     1621 tgccgccgac accgacatgc tcttcgcagt gaccgcgagc gacgagacca acctcgtggc
     1681 gagcaaggtg gggcacgcgg tgttcaacat cccgacgcgc atcgcgcgca tccgctcgac
     1741 gtggttcgag cgccacccgg agctcctcga gggcgactgc ttctccgtga gccacgccat
     1801 ctgccccgag cagatcgtga ccgagtacat cctgcgcctg atcgagttcc ccgaagcgct
     1861 ggaagtggtc gagttcgccg gcggcaaggt ggacctggtg gccgtgcgcg cggtcgtgga
     1921 aggcacgctt gtgcgccacc cgcttcgcga cctcgaggag ctccttccgg acatcggcgc
     1981 gaaggtggcc gcgatctacc gcaacgaccg gccgctcgcg ctctcgcccg acacggtcgt
     2041 tcacccgggc gacgaggtct tcatcctcgc cgacgcgagg gacatccgcg aggtgatgac
     2101 cgagctgcgc aagatggacc ggccggtgcg ccgcgtcatc atcgcgggcg gcggcaacat
     2161 cggcctgcgg ctcgcgcgcg ccatcgaggg ccggatgaac gtgaaggtga tcgaggcctc
     2221 gaagcggcgc tgcgagttcc tcgccggcca gctcgccagc gcgctggtgc tcaacggcga
     2281 cgtgagcaac gaggacctgc tgggcgacga gaacgtggac gagaccgacc tcttcgtttc
     2341 ggtgaccaac gacgacgagg cgaacatcat gtcggcgctg ctcgccaagc gcatgggcgc
     2401 gcgccgggtg atcgcgctga tcaaccgccg cgcctacggc gacctcatgc agggcgggca
     2461 gatcgacatc gccatctcgc ccgcgcaggc cacgctcggc agccttctcg agcacgtgcg
     2521 ccgcggcgac gtggtggccg tgcacagcgt gcgccgcggg cgcgcggagg cgctggagct
     2581 catcgcccac ggcgaccgca agacctcgcg cgtcgtcggc cgcaggatcc gcgagatcga
     2641 cctgccgccc ggggccacga tcgtcgggat catccgcggc gaccgggtca tcgtcgccaa
     2701 ggacgacggg ctggtgatcg agcccgacga ccacgtcctc gtgttcctca cccagacgcg
     2761 catgatcccg aaggtcgaga agctcttcga ggtctcggtg ggattcttct aggcatgagc
     2821 cgctcgatac cccaggagcg ccgggcgcag gaaggcggcc ggcggccggt ggacgcggtg
     2881 ctgcgccact tcccggtgct gcacgtgctg tcggcggcgg tgctgctctt ctcgccggct
     2941 ttcctcgtgc cgctcgtgtt ctccatggcg ctggccgacg gcgcgcacgc ggccttcgag
     3001 acgggcttcc tcgtcacctt cgtgaccggg ctgttcacgt tcttcctgac ccgcaggcgg
     3061 caccgccgcg agctgcagcc gcgcgacgga ttcctcctcg tgtccctcgt gtggtcggtg
     3121 gtgccggctt tcgccacgat tcccctgctg ctcgccatgc ccggcctttc gttcaccgac
     3181 gcctatttcg aggcggtctc gggcatgacc acctccggag ccacagtgct tgccgggctg
     3241 gacgccctcg agccttcggt caacatctgg cgctcgctcc tggtgtggat cggcggcatg
     3301 ggcatcatcg tgctggcggt ggcgatcatg ccgctgctgg gcgtgggcgg cagccagctc
     3361 ttcaaggccg aaagcccggg ctgcatgaag gacaccaagc tcacgccgcg catcggcgat
     3421 accgcgaagg gcctgtggac ggtgtacgcc gccgtcaccg ccgcctgcct cgtctctttc
     3481 cgtctcgccg ggatgacgtg ggtggacgcg gtgatccatg cgttctcgac catggggctg
     3541 ggcggattct cctcccacga cgacagcttc ggctacttca agtccccggc gatcgaggcg
     3601 gtgacgacgt tcttcatgct ggccgctagc ctcaacttcg cgacgcactt cctcgcagtg
     3661 cgcaggagat cgccggcgcc ctacgccgcg gatcccgagg tgcgtcgcgt gctgatctgg
     3721 atggtcgggt cgtcggtcgg catcgcggcc ttcctcgtct tgcgtggcac gtacccggac
     3781 ttcgccacgg cattgcggca cgcttcgttc aacgtggtgt ccatcgcctc gacgacgggc
     3841 ttcgcctcgc tggactacgc gcagtggccg gcattcgcgc ccatctggat gatcttcctg
     3901 tcgtgcttcg ccaccagctc gggctccacg ggcggcggca tcaagatggt ccgtgccgtg
     3961 gtgctgttca agcagacgat gcgcgagatg acgcgcatca tccacccgaa ggccgtgctg
     4021 ccggtgaagt tcggcgagca ggtgatcgag aacaacgtga tcttcgccgt tctcgccttc
     4081 atgctgatgt ggggcgggac gatcatcgtg atgtcgatgc tgcttctcgc gagcggtctc
     4141 gacgtggttt ccgccgtgac cgcggtgctc gcctgcatca acaacatggg cccggggctc
     4201 ggcgccgtgg gcccgtcgtc gaactacgcc ggcctcaccg acttccagac ctgggtctgc
     4261 accgccacca tgctgctcgg gcgcctcgag ctcttcacga tgctcgtgct cttcacgccg
     4321 gcgttctggc ggaagtagcg gttccagcgg tgtcggacgc ggtgcctgac accacgggcg
     4381 ccggcgccgg ccggagcgcg ccgaggatcg cgccggcgag ggaccaggcg agcagtgcgc
     4441 tgtggcgcac gaggaaatcg tcggtcatgt tgcggatcgc gaacccggcg acgagcacca
     4501 gcccgagcgt ggcggcgcag cgccgcggcg gcccggcctt gagcccggcg gccatctcgc
     4561 gcaccagcgc cgccaccatc caggcgaaca ggcccagccc gatcgccccg ccctgcaaca
     4621 gcacattgat cggggtgttg tgcgcgtggg tgtaaagcgg gttgtcgacg cccggagaca
     4681 cgccgttgcg gaactgctgg cgaaggatgc cgcggccgta cccgtggccg atccagggcg
     4741 cctcgccatt cagccgcgcc gcgtggtccc agatcgcgag gcgcgggtcg cgggagagct
     4801 gggcctcggc ggcttgctcc gtatgttcca gcttcgtggt gcgttgctgg atcgagagca
     4861 cgaacagcgc ggagaacgcg atcaaggccg ccgcagtgaa tgcgacgacc tgcgagcgca
     4921 gcggccctgc gagcgatgcg cgtgacagga acacggcgag cagcgtcatc gccgcgagcg
     4981 ccacccaggc gatgcggttg tcgttccacg aggtgacgac gagggtgagc acggcgacgg
     5041 ccgcgagcac gacccgcgcg ccgcggaagg ccgtgccggc gcgcaggaag gcccaggcaa
     5101 ggagcggaag cgccagcacg acgtgcgtgg cgtagtaccc cgcatcgctg tgccagcgtt
     5161 gcgggtccca ccagccatcg aggacaagct ccgccccggc aacgaggccc agcgccgcga
     5221 gcccggcgga aaggctccag gcccagcgat cgatggccgc gacagacggc gtggccgcga
     5281 agaacgcgag gaaggcgatg aaggggtaga gcaacccggg gcgcagctcg gacagcgaat
     5341 aggccggctc gacgctccat gcgacggagg cgatgctcca tgcgccatac gcgaggatcg
     5401 gcgccagcac gcgccacgag ggaagccgcg gcctcgccac catgcctttc gccgacccgt
     5461 agagcacgca ggccgcggca aacccgagca gcgcgttgcg cagcgccgcg ttcgaggaga
     5521 acggcaggac gaacaggaac gcgacgagca cgccggcgac ggccctgttc catgcgggct
     5581 gcgcgtcgac aggtccggaa gatgtcatcg gcgcccgcga gcgcctagac gccgcaccag
     5641 cggcgcatga gcgtggcgaa cgtgtccacg gactgctcga tgcgcgacat cgagcaccac
     5701 tcatccgtct ggtgcgcgag gcggggttcc ccggggccga ggaccacggc gggcgggccg
     5761 ccgtagccct gcttgagata ggcgccgtcc gtggagaagg tgatggtctt cggcgtcggc
     5821 cgcccttcga gaaatggcga gcagacctcg aagacctcct gcacccattc gttggccggc
     5881 tccgtgtaga gcgagggagt gtcgacgaac ggcttcaccg tcgcgccgcg cgggccgagc
     5941 aggtgctcga gggaatggca caggtgagcg tggtcgatgc ccggcacggt gcggatgtcc
     6001 acagtgatct tcgccgaatc gggcaccgag ttggtattga gcccgccgcg gatggtgccc
     6061 acgctgagcg tcgggttgcc catcaccgga tgagactcga cgggaaagcg gaactgctcg
     6121 aggtcgccga tcacgcgcgc catcctgttg atggcgttgt cgcccagctc gggcatggag
     6181 ccgtgcgcgg tgacgcccgt cgtctcgatc tcgaaccaca ccaggccctt gtgccccaca
     6241 tacggatagt tggccgtggg ctcggccacg acgatggcgc cggcgcgatc gagcagcttc
     6301 gcatcggcga ggaacttcga gccctcgcag ccgatctcct cgctcgcggt gatgacgagg
     6361 accacgccgg ggctgcgcgc gagaagcggc gcgagctcga ccgccgccgc cacgaaagcc
     6421 gcgatgccgc tcttcatgtc ggtggagcca cggccgtaga ggcggtcacc gtcggtctcg
     6481 cccgcgaagg cgtccttcga ccacgctttc gcgccgaggg gcacggtgtc gatgtgcccg
     6541 gtgaagcaga tcgggggcct gacgggatcg ccgccgattt cagccacgag gctggtgcgc
     6601 gcctcggcga actcgtggta cgcggtgcgg aacccggcgg cctcgaggat ggcgccgagg
     6661 tggcgcgcgc aggcccgttc catgcccggc gggttgatcg tgttgaaggc gagcagctcc
     6721 ctggtgagcg cccgtggatc gatgcgggta ttcgtcattg ccaacgggtc ctcaggcgcc
     6781 cttgagccgc ttgcggcggc ggcgggccca ctggttgagc gtttccacgg ccacggagaa
     6841 cgcgagcgcg aagtagatgt agcccttggg cacatgcacg cccaggccgt cggcgatgag
     6901 gacgacgccg atgagcagca ggaaggagag cgccagcatc ttcaccgtcg gatgcgccga
     6961 gacgaaattc gccagcgggt cggaggccgc gatcatgatg agcatcgcca tcaccacggc
     7021 caccaccatc acccacaggt ggtcggccat gccgacggcg gtgatcaccg aatcgagcga
     7081 gaagatgatg tcgatgatcg cgatctggat caccacgccc ttgaaggtga cgccggccgc
     7141 gccctcggcc ttgtcggggt ccccttcctc cttctcgatc atctcgtgga tctcgacggt
     7201 ggccttgtag aggaggaaga gtccgcccgc gatgaggatg atgtcgcgcc aggagaaatc
     7261 cttgccggct atcgtgaaga cgggctgcgt gagccccacg atccaggcca gcgtggcgag
     7321 cagcatgagc cgcgtgacga gcgccagcgc gagccccacc ttgcggccca gcgagcgctg
     7381 gtgctccgga aggcgccccg tgaggatcgc gatgaagacg aggttgtcga tcccgagcac
     7441 gatctccagc gcgcagagcg tgacgagcga gagccagatt tgcggatcgg tgatccaggc
     7501 gaaagtgaag aattccatgg agcgtcgttc ctgttgccag gccgccattg tctcatgcgc
     7561 gttgtcgaac cccggcaagc agtcgggaga gcgtccatga tccgcagggc cagctccggc
     7621 ggacgcagca gaagctctac gactacatca accggctgca gaagggaatt ggagcggccg
     7681 ggcaggcgac cacctacgga tacgccgggg acaacagtta ctggaccgta acggatccgc
     7741 ttttccggaa gaccacgaac tacaaggatg tattcgaccg cctcaactac gtcaccgatc
     7801 cgg
//
LOCUS       DASUEV010000096         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1516290, whole genome shotgun sequence.
ACCESSION   DASUEV010000096 DASUEV010000000
VERSION     DASUEV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516290"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1108
                     /locus_tag="VFV55_03045"
     CDS             143..1108
                     /locus_tag="VFV55_03045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX4943301.1"
                     /translation="MKTTFLDFEQPIAELEQKIEELRFVQDDSAVDISQEIERLSKKS
                     RDLTKDIYAKLSSWQISKVARHPQRPYTLDYIAAMCTDFEEMHGDRSFSDDAAIVGGL
                     ARFNGQSVMVLGHQKGHDTNEKILRNFGMPRPEGYRKALRLMKLAEKFAIPLLTFIDT
                     PGAYPGIGAEERGQSEAIGRNLYEMAGLRVPIVVSVIGEGGSGGALAIAVGDATLMMQ
                     YSTYSVISPEGCAAILWKSPENAAEAAECLGITAPRLKSLGLVDKVVNEPLGGAHRDP
                     AEAAKSLKKAIADNLRILQDLPMTQLLERRQDRLVNYGKFKELAE"
     gene            1129..2553
                     /gene="tilS"
                     /locus_tag="VFV55_03050"
     CDS             1129..2553
                     /gene="tilS"
                     /locus_tag="VFV55_03050"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX4943302.1"
                     /translation="MPPRPVLPEGLTARLRRRLAAHVRRGQSVCVAYSGGLDSTVLLD
                     LLARLRAETGFTLSALHVHHGISRNADRWAEACAAFAHARGVPLVIARVTVDRDHPAG
                     LEAAARASRYAAFARAGTDFVALAHHRDDQAETVLLQALRGTGMKGLSAMPEARAADG
                     SVWIRPLIDEPRDVLLAHAREAGLDWVEDESNELTAFDRNFLRHEIMPALERRFPQYR
                     ESLARLARHAATASGLLEELAREDTARITDRDGLSAAGLGSLDAARRANVLRHFLAGH
                     GLAMPGEARLADMSRQLVSARDDARILLLHGGRALVRHQGRIVIEEAPGEAAGWDVAW
                     HGERVFSLGEGRGEVRFADAAGEGIAVERVGGEGWHFAPRAGGERIRLHDGAPTRTLK
                     NLLQERAVPVWRRSRLPLLFEGDRLVWVPGIGIAAGYRAKGTAAGLIPDWLSGTVPEN
                     RPRNLSRTPAAPSPGAPPCDKMKS"
     gene            2582..3007
                     /gene="ndk"
                     /locus_tag="VFV55_03055"
     CDS             2582..3007
                     /gene="ndk"
                     /locus_tag="VFV55_03055"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX4943303.1"
                     /translation="MAVQRTLSIIKPDAVAKNIIGKIYSRFETNGLKVIAAKMVHLSR
                     QEAEGFYAVHRARPFFKDLVEFMISGPVMIQVLEGEQAVQKNRDLMGATDPKKAEKGT
                     IRADFADSIDANAVHGSDAPETAATEIAYFFPASQVYSR"
     gene            3038..>3354
                     /locus_tag="VFV55_03060"
     CDS             3038..>3354
                     /locus_tag="VFV55_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX4943304.1"
                     /translation="MRTNLLDFTLPALTDWFASLGEKPFRARQVFRWVHQRGESDFDA
                     MTDLAKSLREKLRETAEVRPPAILSEHRSADGTVKWLFDVGIGNGIETVFIPEDDRGT
                     LCVS"
BASE COUNT          534 a         1182 c         1128 g          510 t
ORIGIN      
        1 cgccgggacc catcccgggg ccacgcctgc gcgctccgaa aatgcaaacg catccttcgc
       61 ctgcctcgcc cttcgccctg cccacctgcc tcgcacctgc caccctgtta tcatttccgc
      121 cattcgcatc gccggccacc aagtgaaaac caccttcctg gatttcgagc agccgatcgc
      181 ggaactcgag cagaagatcg aggagctgcg cttcgtgcag gacgacagtg ccgtcgacat
      241 ctcgcaggag atcgagcggc tgtcgaagaa gagccgcgac ctcacgaagg acatctacgc
      301 gaagctctct tcgtggcaga tctcgaaggt cgcgcgccat ccgcaaaggc cgtacacgct
      361 cgactacatc gcggccatgt gtaccgattt cgaggaaatg cacggcgacc gctccttctc
      421 ggacgatgcc gcgatcgtgg gcgggctggc gcgcttcaac ggccagagcg tgatggtgct
      481 gggccaccag aagggccacg acaccaacga gaagatcctg cgcaacttcg gcatgccgcg
      541 tcccgagggc taccgcaagg cgctgcgcct catgaagctc gcggagaagt tcgcgatccc
      601 gctgctcacc ttcatcgaca cgcccggcgc gtatcccggc atcggcgccg aggagcgcgg
      661 gcagtcggag gcaatcgggc gcaacctcta cgagatggcc ggactgcgcg tgcccatcgt
      721 ggtttcggtc atcggcgagg gcggttccgg cggggcgctg gcgatcgcgg tgggcgacgc
      781 gacgctgatg atgcagtact ccacctactc cgtgatctcg cccgagggct gcgcggccat
      841 cctgtggaag agcccggaga acgcggccga ggcggcggag tgcctgggca tcacggcgcc
      901 gcgcctgaag tccctgggcc tcgtcgacaa ggtggtgaac gagcctctcg gcggcgccca
      961 ccgcgacccg gccgaggcgg ccaagtccct caagaaggcg atcgcggaca acctgcgcat
     1021 ccttcaggac ctgccgatga cccagctcct cgagcgccgc caggaccggc tcgtgaacta
     1081 cggcaagttc aaggagctgg cggagtagcg cgcacggcgc cgtctccgat gcccccacgc
     1141 cccgttctcc ctgaaggact cacggcgcgc ctgcggcggc gactcgccgc gcacgtgcgg
     1201 cgcggccagt cggtgtgcgt cgcctactcg ggcggcctcg actccaccgt cctgctcgac
     1261 ctccttgcgc gcctgagggc ggaaaccggg ttcacgctct ccgcactgca cgtccatcac
     1321 ggcatttcgc gcaatgccga tcgctgggcc gaggcttgcg ccgcgttcgc gcacgcgcgc
     1381 ggcgtgccgc tcgtgatcgc gcgcgtgacg gtcgatcgcg accaccccgc gggcctggaa
     1441 gcggcggcgc gcgcgtcgcg ctacgcggcc ttcgcccggg ccggcaccga tttcgtggct
     1501 ctggcgcacc accgggacga ccaggccgag acggtgctgc tccaggcgtt gcgcggcacc
     1561 ggcatgaagg ggctctcggc gatgcccgag gcgagggccg cggacgggtc ggtgtggatc
     1621 cggccgctca tcgacgagcc gcgcgacgtg ctcctggccc atgcgcgaga ggcggggctc
     1681 gactgggtcg aggacgagtc gaacgaactc accgcgttcg atcgcaactt cctgcgccac
     1741 gagatcatgc ccgcgctcga gcggcgcttt ccgcagtatc gcgagtcgct cgccaggctg
     1801 gcccggcatg cggccaccgc gagcgggctt ctcgaggaac tcgcgcgcga ggacacggct
     1861 cgcatcaccg accgggacgg gctctccgcc gccggcctcg gttccctcga cgccgcgcgc
     1921 cgggccaacg tgctgcgcca cttcctcgcg ggccacgggc ttgccatgcc gggggaggcg
     1981 cggctcgccg acatgtcgcg ccagctcgtt tccgcccgcg acgacgcgcg catcctgctg
     2041 ctgcacggcg gccgggccct ggtgcgccat caggggcgca tcgtcatcga ggaggctccc
     2101 ggcgaggcgg ccggatggga tgtcgcgtgg cacggcgagc gtgtgttctc cctcggcgaa
     2161 ggtcgcgggg aagtgcgttt tgcggacgcg gcgggcgaag ggatcgcggt cgagcgcgtg
     2221 ggcggagagg gctggcactt cgcgccgcgc gcgggcggcg agcgcatccg ccttcacgac
     2281 ggcgcgccca cccggacgct caagaacctg ttgcaggagc gcgcggtgcc ggtctggcgg
     2341 cggtcgcgcc tgccccttct cttcgagggc gatcgcctgg tatgggtacc cgggatcgga
     2401 atcgcggccg gctaccgcgc caagggcacg gcagcgggac tcatcccaga ctggctttcg
     2461 gggacggttc cggagaaccg tccccgaaac ctgtcccgga caccggccgc ccctagcccg
     2521 ggagcgccgc cctgtgataa aatgaaaagc taaatttttc attcagttag cggagatttt
     2581 catggcggtt cagcgcacct tgtcgatcat caagcccgat gccgtggcca agaacatcat
     2641 cggcaagatc tactcccgtt tcgagaccaa cgggctcaag gtcatcgcgg caaagatggt
     2701 ccatctctcc cggcaggaag cggaagggtt ctacgccgtg caccgggccc gcccgttctt
     2761 caaggacctc gtggaattca tgatttccgg cccggtgatg atccaggtgc tcgaaggcga
     2821 gcaggcggtg cagaagaacc gcgacctcat gggcgccacc gacccgaaga aggcggagaa
     2881 aggcacgatc cgcgccgatt tcgccgactc catcgatgcc aacgccgtgc acggctcgga
     2941 tgcgccggag actgccgcga cggagatcgc ctacttcttc ccggcgagcc aggtctattc
     3001 gcgctgagcg ggccgcaagg ccgcgcgcga caagtccatg cggaccaacc tgctcgactt
     3061 cacgcttccc gccctgaccg actggttcgc gtcgctgggc gagaagccgt ttcgcgcccg
     3121 ccaggtgttc cgctgggtgc accagcgcgg cgagtccgac ttcgacgcga tgaccgacct
     3181 tgccaagtcc ctgcgcgaga agctccgcga aacggccgag gtccgcccgc ccgccatcct
     3241 ctccgagcac cgctcggccg acggcaccgt gaagtggctc ttcgacgtgg gcatcggcaa
     3301 cggcatcgag acggtgttca tccccgagga cgaccgcggc acgctgtgcg tgtc
//
LOCUS       DASUEV010000097         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1395175, whole genome shotgun sequence.
ACCESSION   DASUEV010000097 DASUEV010000000
VERSION     DASUEV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395175"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFV55_03065"
     CDS             <1..894
                     /locus_tag="VFV55_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX4943305.1"
                     /translation="TMEKWGLSYESLSRSNPGLVMVRLSGFGQTGPYKDKPGFGAVGE
                     SMGGMRYITGYPDRAPVRVGISIGDSLAALFGVIGALTAIVHRGNTGKGQVVDVALYE
                     AVFAMMESMLPEYGMGGTIRERAGSALPGIVPSNTYRCRDGKYVVIGANGDSIFKRMM
                     RMIGRPDLADDPSLASNDGRVRRTEELDRVIGEWTATVGLDEALVKLEGAEVPSGRIY
                     SIADIVADVHYQARGMIEKHRLDAGNDILLPGIVPRLSDTPGATKWIGPRLGEHTDAV
                     LREAGYSEADVRRLREAKVIA"
     gene            complement(940..>3645)
                     /gene="ppc"
                     /locus_tag="VFV55_03070"
     CDS             complement(940..>3645)
                     /gene="ppc"
                     /locus_tag="VFV55_03070"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX4943306.1"
                     /translation="RLGRILGDTLKQHEGEETYELIETIRKLAVASPRVEDVTSRRAL
                     AGTLDALTDDQTVLVVRAFSFFSLLANIAEDRHHIRRQRENRRHGSRPLDSTLRGLFA
                     DALERGTTRAEGMATLSRVRVTPVLTAHPTEVQRRSTLDIQLAIADWLGRLDNPDLLE
                     EELAAFEAELRRLVSTLWQTRMLRAVKLGVKDEIENALAYFDYSFIDAVPRIHEEIED
                     SVAALPGEGAVAPLPTVLSIGSWVGGDRDGNPFVTAEMLEHAFRRQAEIAFDHYFAEV
                     HALGAELPISGLLVQLTPDMKALSEHSRDPSLFRQDEPYRRALTGVFARLAATAAALG
                     LKREHRTAVGKGEPYADCAAFVADLDVIDASLRTGNSARLAGGRLRHLRRAARTFGFH
                     LATVDLRQNADVHERVIAELFRCARVAPDYLALEEGARQKLLRADLATPRPLRSPFAA
                     YSELTRGELAIVEQAAKVHSSLGPESIRQYVISKTESVSDLLEVAVMLKEAGLVVPGE
                     APVAKMQIVPLFETIGDLRRAPDTMRAWFALPEARSIVSSLGGVQEVMLGYSDSNKDG
                     GYVTSNWELYKAETELVAVFREAGVRLRFFHGRGGTVGRGGGPSYEAILAQPPGSVQG
                     ELRLTEQGEVIASKYANRDIGRRNLEALLAATVRATLDEAPHEEHAEYHAAMEEISAF
                     AIAAYRGLVYETPGFVDYFRASTPIREIAELNIGSRPASRKPSQRIEDLRAIPWVFSW
                     AQCRVMLPGWYGLGSAVEAFVAKHGEEGRGLLADMWLNWPFFRAMLSNLEMLLAKADL
                     SVAARYKELVPDARLADEIFDRIRRELDLTVKAFFTITTNEAFLVTNPSLARSIRNRF
                     PYLDPLNHLQVELLKRYRAGDSADKVHRGIHLTINGLAAGLRNSG"
BASE COUNT          570 a         1238 c         1274 g          563 t
ORIGIN      
        1 acgatggaaa aatggggcct gtcctatgag tcgctctcca ggtccaaccc ggggctggtc
       61 atggtgcggc tatccggctt cggccagacg ggcccctaca aggacaagcc gggcttcggc
      121 gccgtcggcg aatccatggg cggcatgcgc tacatcaccg gttaccccga tcgcgcgccg
      181 gtgcgcgtgg gcatctcgat cggcgattcg ctcgcggccc tgttcggcgt gatcggggcg
      241 ctcaccgcga tcgtccatcg cggcaacacg ggcaagggcc aggtcgtgga cgtggcgctc
      301 tacgaggcgg tgttcgcgat gatggaatcg atgcttccgg agtacggcat gggcggcacc
      361 atccgcgagc gggcgggctc ggcgctgccc ggcatcgtgc cctccaacac ctaccgttgc
      421 cgcgacggga aatacgtcgt catcggcgcc aacggcgact ccatcttcaa gcggatgatg
      481 cgcatgatcg ggcgccccga cctcgcggac gacccgtcgc tcgccagcaa cgacgggcgg
      541 gtcaggcgca cggaggagct cgaccgcgtg atcggcgaat ggacggcgac cgtcggtctc
      601 gacgaggccc tcgtgaagct cgagggggcc gaggtgccct cgggacgcat ctactccatc
      661 gccgacatcg ttgccgacgt ccactaccag gcgcgcggca tgatcgagaa gcaccggctc
      721 gacgcaggga acgacatcct gctgcccggc atcgtgccca ggctctcgga cacgcccggc
      781 gcgacgaaat ggatcggccc gaggctgggc gagcacaccg acgccgttct gcgcgaggca
      841 ggctattccg aggccgatgt ccgccgcctt cgcgaagcaa aggttatcgc gtaggtaaca
      901 ttggggacgg ttccggagaa ccgtccccaa tacctgtccc tatcctgagt tgcgcaggcc
      961 cgcggccagg ccgttgatgg tgaggtggat gccgcggtgc accttgtccg cggaatcgcc
     1021 ggcgcggtag cgcttgagga gctcgacctg caggtggttc aggggatcga ggtaggggaa
     1081 gcggttgcgg atggagcgcg ccagggacgg gttggtcacc aggaacgcct cgttggtggt
     1141 gatggtgaag aaggccttca cggtcaggtc cagctcgcgg cggatgcgat cgaagatctc
     1201 gtcggccagg cgcgcgtcgg gcaccagttc cttgtagcgc gcggccaccg acaggtccgc
     1261 cttggcgagg agcatctcca ggttggagag catggcgcgg aagaagggcc agttgagcca
     1321 catgtcggcc agcagcccgc gcccctcctc gccgtgcttg gcgacaaagg cctccacggc
     1381 gctgcccagc ccgtaccagc ccggcagcat cacgcggcat tgcgcccagg agaacaccca
     1441 gggaatggcg cgcaggtcct cgatgcgctg cgagggcttg cgggaggccg gccgcgaacc
     1501 gatgttgagc tccgcgatct cgcgaatcgg cgtggaggcg cggaagtaat cgacgaaccc
     1561 gggcgtctcg tataccagtc cccggtacgc ggcgatggcg aacgcggaga tttcctccat
     1621 cgcggcgtgg tactcggcgt gctcctcgtg cggcgcctcg tcgagggtgg cgcgaaccgt
     1681 ggcggcgagc agcgcctcga ggttccggcg cccgatgtcg cggttggcgt acttgctcgc
     1741 gatgacctcg ccctgttcgg tgaggcgcaa ctcgccctgc acactgccgg gcggctgcgc
     1801 gagaatcgcc tcgtaggacg gcccgccccc gcgccccacg gtgccgccgc gcccgtggaa
     1861 gaagcgcagc cgcacgcccg cctcgcggaa caccgccacc agctccgtct ccgccttgta
     1921 gagctcccag ttggaagtga cgtaaccgcc gtccttgttg ctgtcggagt agccgagcat
     1981 cacttcctgc accccgccca gcgaagagac gatcgatctt gcttccggaa gtgcgaacca
     2041 cgcgcgcatc gtgtccggcg cccggcgcag gtccccgatg gtctcgaaga gcggcacgat
     2101 ctgcatcttc gcgacgggcg cctcgcccgg aaccacgagc cccgcttctt tcagcatcac
     2161 cgcgacttcg agcaggtccg acacgctttc ggtcttggag atcacgtact ggcggatcga
     2221 ttcaggcccc agggaggaat gcaccttcgc tgcctgctcc acgatggcga gctcgccccg
     2281 ggtgagttcc gaataggcgg cgaaaggcga gcgcaatggc cgcggcgtgg ccaggtccgc
     2341 gcgcagcagc ttctgccgtg ccccctcctc gagcgccagg taatcgggcg ccacgcgcgc
     2401 gcagcgaaac agctccgcga tgacgcgctc gtgcacatcc gcattctggc gcaggtccac
     2461 ggtggccagg tggaagccga aggtgcgcgc ggcccggcgc aaatggcgca ggcggccgcc
     2521 ggccagccgc gccgagttgc ccgtccgcag gctcgcgtcg atcacgtcga ggtcggccac
     2581 gaacgccgcg cagtccgcat acggctcgcc cttgcccacg gcggtgcggt gctcgcgctt
     2641 gagccccagc gcggcggccg tcgcggcgag cctcgcgaac acgcccgtga gggcccggcg
     2701 atacggttcg tcctggcgga agagcgaggg gtcccgggaa tgctccgaca gcgccttcat
     2761 gtccggcgtc aactgcacca gcagcccgga aatgggcagc tccgccccca gcgcgtgcac
     2821 ctccgcgaaa tagtggtcga acgcgatctc ggcctggcgg cggaaggcgt gctcgagcat
     2881 ctcggcggtg acgaacgggt tgccgtcgcg atcgccgccc acccagctgc cgatggacag
     2941 cacggtcggc aggggcgcga cggcgccctc gcccggaagt gcggccaccg aatcctcgat
     3001 ctcctcgtgg atgcgcggca cggcgtcgat gaatgagtag tcgaagtagg cgagcgcgtt
     3061 ctcgatctcg tccttcacgc cgagcttcac ggcgcgcagc atgcgcgtct gccagagggt
     3121 ggacaccagc cgccggagct ccgcctcgaa ggctgccagc tcctcttcca gcaggtccgg
     3181 gttgtcgagc cggcccagcc aatcggcgat ggcgagctgg atgtccagcg tgctgcggcg
     3241 ctgcacttcc gtgggatggg cggtcagcac gggcgtcacg cgcaccctcg acagcgtggc
     3301 cattccctcg gcgcgcgttg tgcccctttc cagcgcatcg gcgaagaggc cgcgcaaggt
     3361 ggaatcgagc ggtctcgacc cgtggcgccg gttctcccgc tggcggcgga tgtgatggcg
     3421 gtcctcggcg atgttggcca gcagcgagaa gaagctgaat gcccgcacga ccagcacggt
     3481 ctggtcgtcg gtcagggcgt cgagcgtccc ggcgagcgcg cggcgggagg tcacgtcctc
     3541 cacgcgcggg ctcgccacgg cgagcttgcg gatggtttcg atgagctcgt aggtctcctc
     3601 gccctcgtgc tgcttgaggg tatcgccgag gatccggccc agccg
//
LOCUS       DASUEV010000098        18878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_242680, whole genome shotgun sequence.
ACCESSION   DASUEV010000098 DASUEV010000000
VERSION     DASUEV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 18878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18878
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242680"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="VFV55_03075"
     CDS             <1..1636
                     /locus_tag="VFV55_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4943307.1"
                     /translation="GAATTTARAMTKSPAPVTQAMLNAAGGNAGNWIHSNGSYEQTRF
                     HPGKLINTGNVAKLKPAFVFQTAVLESMETAPVVVDGVMFLTTSFNHVYAIDAATGEE
                     FWHYKHKLGPIVTVCCGNNNRGVAIAGGTLYMGTIDAKLVALDAKTGKLLWETQIADP
                     EKGYSETMAPVAVDGKILIGTNGGEYGIRGFVKAFDARDGKNLWTFHTIPETGHEGVW
                     AARDATGRDMHRDIAAEKAALAKDSSFYKALGGGVWMAPAVDLKTRTVFFVVGNPSPD
                     LYGAIRPGDNLYTDSIVAVDLDKGTYKWHFQYIAHDVWDLDAVSPPILTQAKNAAGKI
                     VDVVIHGGKTGHIYVHERDTGKLIRFSDAMVPQENMWVLPTKQGARMLPGANGGVEWS
                     PMAVDPKARVAYAANLHQPMTYHVEETPYPGGKLWLGGAFKTIPGEEQWGNLVAVNLD
                     NGKLVWQHKTPQPLIGGVLATAGGLVFNGEANGWFKAFDAKNGKELWKFNCGAGVNAP
                     AVSYTVNNKQYVAVAAGGNNQIDSKRGNSVFVFALP"
     gene            1703..2284
                     /locus_tag="VFV55_03080"
     CDS             1703..2284
                     /locus_tag="VFV55_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4943308.1"
                     /translation="MKPLAAALACAANLILVFPSAAQDDPAVRKAAEPCFACHGPEGN
                     SVNPAYPILAGETWRYIYIQLKDFKEGRRADPLMSPMAANLTRDDMIALGNFFAAQKR
                     RPNGFKADGARVAAGKKISDAVLCPMCHLGGFSGQNDIPRVAGQHPDYIRKQLRDFKA
                     RKRTNDAGNMTSVAANLSDDDIENLTHYIANLN"
     gene            2384..3076
                     /gene="phoB"
                     /locus_tag="VFV55_03085"
     CDS             2384..3076
                     /gene="phoB"
                     /locus_tag="VFV55_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX4943309.1"
                     /translation="MPAHILVVEDEPAIQELIALNLRQAGQRAVLADSAEQAMEQIRE
                     ELPDLVLLDWMLPGQSGLELARRLRGDARTRNLPIIMLTARGGEEDKLRGLETGADDY
                     ITKPFSVKELQARIKAVLRRRAPEITDDVVDYDGLRLDPASHRITGKGRELPMGPTEF
                     RLLHFFMTHPERVHTRTQLLDQVWGDHVFIEERTVDVHIRRLRSALAKSGHHALIQTI
                     RGSGYRFSSATK"
     gene            3073..4407
                     /gene="phoR"
                     /locus_tag="VFV55_03090"
     CDS             3073..4407
                     /gene="phoR"
                     /locus_tag="VFV55_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEX4943310.1"
                     /translation="MKDWIGRLASIAAIAGLGALLTGYIAGAEFAALFLFAFLVIVVS
                     IGLSGRARLERWLAEPSVDDLPEGEGPWDEIYARLHRILRQQSNTRASLSHALWRFRQ
                     AGEAMPDGVIVLDGGNRIEWMNPSAENHFGLAFKDDRGQAITNLIRQPSFVSYLDARR
                     YGEPLTLKGASEADRILSVQLVPYGDREKLLLSRDVTRWERLEAVRRDFIANVSHELR
                     TPLTVLKGFLETLTDARQADEKLYRRSLELMTDQAERMQRLVEDLLMLSRLEDTRYPL
                     REEPVDVPALLQSVLVEAESINQGRHRIASKIEPAWLLASREEIRSAITNLLTNAIRY
                     TPAGGDIAVTFAIENGEPTLRVRDNGEGIAPEHIPRLTERFYRVDRSRSRSTGGTGLG
                     LAIVKHVLQRHQGRLDIASEVGAGSTFSCVFPATRIASPSGSPAQDTPVRAA"
     gene            4476..4991
                     /gene="fabA"
                     /locus_tag="VFV55_03095"
     CDS             4476..4991
                     /gene="fabA"
                     /locus_tag="VFV55_03095"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX4943311.1"
                     /translation="MTQKSAYNRDELIACGQGRLFRPDSGRLPLPPMLMFDRITRIND
                     DGGSEGKGEIVAELDVKPDLWFFGCHFESDPVMPGCLGLDALWQLVGFYLVWRGNPGR
                     GRALGSGEVKFVGQVLPTAKLVTYRLTLKRLIERKLVLGIADGSVLVDGREICTAIDL
                     KVGLFTSTDAF"
     gene            4991..6205
                     /gene="fabB"
                     /locus_tag="VFV55_03100"
     CDS             4991..6205
                     /gene="fabB"
                     /locus_tag="VFV55_03100"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX4943312.1"
                     /translation="MRRVVITGLGIVSCLGNDKQGVSASLREMRSGITHQPEYATMGF
                     RSQVAGAPVIDLEAAIDRKVRRFMGQAAAYAYVSMKDAIADAGLTPDMVSNPRTGLIT
                     GSGGGSPENQVEAADTARSKGVKRIGAFMVPRTMTSTTSACLATPFGIKGINFTIASA
                     CATSAHCIGESAEKIALGKQDVMFAGGGEELHWSLSCLFDAMGALSSGRNDTPAEASR
                     AYDAGRDGFVIAGGGGMVVLEEYEHARKRGARIYAEVVGYGATSDGYDMVAPSGEGAV
                     RCMRMALETVQGPIDYLNTHGTSTPVGDTAELGAIREAFASGGTAPAFSSTKSLTGHS
                     LGAAGVQEAIYCLLMMEGGFIAGSANVTNRDPAVGDLPLVTATRDAKLTTVMSNSFGF
                     GGTNATLVLRRV"
     gene            6266..6658
                     /locus_tag="VFV55_03105"
     CDS             6266..6658
                     /locus_tag="VFV55_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEX4943313.1"
                     /translation="MIGILLVSHGAFGESLIHCASHVLGRRPLYVRQLGVTVHDVPEE
                     ILPTAEDLIRFLDQGQGVLVMTDIYGATPSNIAAKLLKPGRVEGLAGVNLPMLIRALT
                     YREEPLETAVAKALSGGTEGVMRMFPEA"
     gene            6673..6942
                     /locus_tag="VFV55_03110"
     CDS             6673..6942
                     /locus_tag="VFV55_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX4943314.1"
                     /translation="MQKQTITIVNKLGLHARASAKLTQTAARYPCGVHLSRNGKRVNA
                     KSIMGVMMLAAGIGATVEIETDGPDEEAAMEALVKLFADKFDEGE"
     gene            6956..8719
                     /gene="ptsP"
                     /locus_tag="VFV55_03115"
     CDS             6956..8719
                     /gene="ptsP"
                     /locus_tag="VFV55_03115"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX4943315.1"
                     /translation="MAADGPAPTAHNVCFTMHGIGVSGGIAIGHTHLFLGMSTEVDHY
                     DIAYHDVGREQRRYDRAVKEVRDELKVLADSVRHSAASELAPFVNVHLMLLEDVAFSE
                     APRKIIAEQRCNAEWALRAQLDELSAQFDDIEDEYLREREADVRQVAERVLTALAGSK
                     RRIAIEPTRQDTDLILVARDLSPADVILFREHPFAAFVTDMGGATSHTAIIARSLAIP
                     AIVALHHARTMIREDELVIVDGTQGVLIVNPDRAVLAEYRLKQNQARLEKDKLKRLKS
                     APTATLDGTAVQLQANIELPSDLDAVRASGAAGIGLFRSEFLFMNRRELPDEDEQYEA
                     YRQVVEGMKGRPVTIRTLDIGADKALDGQEHQTENPALGLRAIRYCLAEPQIFNTQLR
                     AILRASRHGKAMILIPMLSSYAELHQAVAAVQRAKEELTVEGVKFDPLVPVGGMIEIP
                     AAAIALPMFIRKLDFLSIGTNDLIQYTLAIDRADDAVAHLYDPLHPAVLSLIAGVIRT
                     ALQHDKPVAVCGEMAGDVELTRLLLGMGLRRFSMHPASLLAVKQRVLSTSLPDIAALV
                     ARVMKADEPEKLISLIEKLNA"
     gene            8722..9150
                     /locus_tag="VFV55_03120"
     CDS             8722..9150
                     /locus_tag="VFV55_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943316.1"
                     /translation="MLREVSAVAQDIPGLTRRWFRDEYFDLYLWEGHGGEIGALQLCY
                     DRSDKERSLRWSAEKGFLHEGVDRPETKPGRAMTAILVVDGAMPLAAVSRKLLAAAKE
                     LPAGIRDFLFARLEEYARGGRPPTPGGRGAPRHGGRERLE"
     gene            9280..10506
                     /locus_tag="VFV55_03125"
     CDS             9280..10506
                     /locus_tag="VFV55_03125"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX4943317.1"
                     /translation="MNDIPPGSVGEVAPQRAGFLEPLILKGGKTLASYELVYETYGTL
                     NAERSNAVLVCHALSGNHHVAGWHADSPKKAGWWDNMVGPGKAIDTERFFVVGVNNLG
                     GCHGSTGPTSIDRATSQPYGAGFPLVTVEDWVASQARLMDRLGVERLAVAMGGSLGGM
                     QALQWTLEFPERIAHAIVIAAAPKLSTQNIAFNDVARQAIVSDPDFHGGNFYAMKTVP
                     ARGLKLARMLGHITYLSEDLLGERFGREIKGDDYGFNYEVEFEIESYLRYQGDKFAQV
                     FDANTYLIMTKALDYFDPARETGGDFARAMAAARAGFLVVSFASDWRFPPQRSREIVK
                     ALVKGGKNVTYAEIDAPHGHDAFLLDNAHYHRLVAAYMDNVAAEVGAGRRARPLIVGG
                     TELEEMKGYAGKGRPS"
     gene            10503..11087
                     /gene="metW"
                     /locus_tag="VFV55_03130"
     CDS             10503..11087
                     /gene="metW"
                     /locus_tag="VFV55_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX4943318.1"
                     /translation="MTPRFDFDLISSWIPEGARVLDLGCGDGDLLAGLAATRGVKGYG
                     VEIDPERMLAAVGHGVNVIQVDLESGLYAFEDDAFDFVILSQTLQAMRRSDHILAEML
                     RVGREGIVTFPNFGYWRHRLSILMGRMPVSESLPYQWYDTPNIHLCTVKDFEDLCVKV
                     GAEILDERVLHEGQVVEVLPNLFGSLGVFRFKRK"
     gene            complement(11137..11556)
                     /locus_tag="VFV55_03135"
     CDS             complement(11137..11556)
                     /locus_tag="VFV55_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX4943319.1"
                     /translation="MAVTHCLDTNVLLYAFSKAPRDAAKARIAREWIAREDWGVTVQV
                     LQEFYVNAVRTPHELGHDDALAMIEEIADSRPVGATDLPLMRHALQLKNRHGIAYWDA
                     AVIAGARRLGATVLVSEDLSHGQDYGGVRVMNPFAGA"
     gene            complement(11559..11864)
                     /locus_tag="VFV55_03140"
     CDS             complement(11559..11864)
                     /locus_tag="VFV55_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX4943320.1"
                     /translation="MTVNLSVKNVPEALAAMLRARAERNHRSLQGELMAILEAAANDP
                     QMARAHSVAAAYDSTPRPAQSLLDRLVAIAGDRGIDTTKRLTREQAHDRQRLRKSGI"
     gene            complement(11916..13190)
                     /locus_tag="VFV55_03145"
     CDS             complement(11916..13190)
                     /locus_tag="VFV55_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4943321.1"
                     /translation="MASHDYSAVFRNRNLAAILLLAFSSGLPLALTGGTLQAWMTVEG
                     VDLSTIGFFTLVGLPYTWKFLWAPMMDRYVPPFLGRRRGWLLLTQLALAAGIAAMAFA
                     SPKGNLAWLAALAVFVAFTSASQDIVFDAYRTDVAQPEQRGLAAAFTVVGYRIAMLTS
                     GAAALVLVGGSGFIPALGWQDTYLLMAALMVVGAMATLWGPEPQAGAPPPRTLDEAVW
                     APLQEFFSRPGAWVLLALIVLYKLGDAFAGSLTTAFLLRGAGFSLDDVGYVNKGMGLA
                     ATIFGALFGGALMVRLGLYRALMAFGILQAVSNLAFMALAAAGKSWPLMLFAVGFENL
                     CGGMGTAAFVALLMALCDHRFTATQYALLSALAAFGRVYVGPAAGYATDPRFLGLSWT
                     VFFFLTFLVALPGLVLLWWKREALRSLDRPVD"
     gene            13290..13682
                     /gene="msrB"
                     /locus_tag="VFV55_03150"
     CDS             13290..13682
                     /gene="msrB"
                     /locus_tag="VFV55_03150"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX4943322.1"
                     /translation="MAKIVRTNEEWKKLLTPQQFEVTRKHGTERPFANEYNDCKVPGT
                     YQCVCCGQDLFASDAKFDSGTGWPSFWQPAAPGAVLEKDDSSWFVKRTEVVCANCDAH
                     LGHVFDDGPKPTGMRYCMNSAALKLKPR"
     gene            complement(13669..14046)
                     /locus_tag="VFV55_03155"
     CDS             complement(13669..14046)
                     /locus_tag="VFV55_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943323.1"
                     /translation="MHLESALPIVKALADGVNPVTGESYPEGSPYAEPRALRALFSAV
                     DLMQREVEREKRRERLPANFGKPWSEGEDHAVTAAFDAGLTIPEVARKHARTQGSIRL
                     RLEKLGKLPPSGEARYDNGPSAA"
     gene            complement(14103..16013)
                     /locus_tag="VFV55_03160"
     CDS             complement(14103..16013)
                     /locus_tag="VFV55_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4943324.1"
                     /translation="MLRLSNITLARGARVLLKEASLTVFPGHRVGLVGANGAGKSSLF
                     ALVLGELHQDAGEVELPARWVLAHVAQELHDTDRAALDFVMDGDTELRQVEAAIREAE
                     AAHEEGERAAMLHGRYDEIGGYAARSRAQALMSGLGFAPEAEASAVAMFSGGWRMRLN
                     LARALMCRSDLLLLDEPTNHLDLDAVMWLEDWLKAYPGALLLITHDREFLDSVVGTIV
                     HFDAQKLTSYAGNYSAFEVQRADRLAQQQAAHERQQRTIAHLHSFIDRFRAKATKARQ
                     AQSRIKALEKLEVIAAAHVDNPFTFAFREPPVKPKLLFRVAEASVGYGDKAVVSDIEW
                     SVYFGEAIGLLGPNGAGKSTLLKTIVGDLPPVAGAIIRSPDLRVGYFAQHQVEKLRLE
                     ESVMWHLARLAAGVREQELRDFLGGFDFRGDQVFQRVADLSGGEKARLALALIVWERP
                     NLLVLDEPTNHLDIEMREALAQALQDFEGTLIVVAHDRHLLEAATDQWWLVADGKVAP
                     FDGNLDDYREWSRQYRARGTKSEGTGAGADRKAQKRAQAEARQKLADAKKPFEKRIGT
                     IERELAKLQPEKEALDAWLASGEAYGEAMRAELAERSKRHGEIAARIAQLEEDWLWTN
                     AAMDVEVNRARE"
     gene            complement(16013..16786)
                     /locus_tag="VFV55_03165"
     CDS             complement(16013..16786)
                     /locus_tag="VFV55_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX4943325.1"
                     /translation="MRIVTLNTNGIRSAASKGLLDWMKRQKADLVCLQEIKAQEPDLP
                     REILAPGKLHAFFHPAEKKGYSGVAIYARKEPDRAELGLGIRDIDAQGRYLQLDYGNL
                     SVVSIYVPSGSSGEEAQARKFSFMKRFMPKLKAMRASGREFILCGDWNIAHKEIDLKN
                     WRSNQKNSGFLPEERAWLTQAFDTVGWVDVFRTLNPLPEQYTWWSNRGRAREKNVGWR
                     IDYHIATPGIARTAEYARIYKDRWFSDHAPLTIDYGYTL"
     gene            16852..17514
                     /gene="pyrE"
                     /locus_tag="VFV55_03170"
     CDS             16852..17514
                     /gene="pyrE"
                     /locus_tag="VFV55_03170"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX4943326.1"
                     /translation="MDATAPAAGQDFRRQFVEFAIRAGVLRFGEFTTKAGRLSPYFFN
                     AGLFHDGASLGELSRYYARAALASGIAFDLLFGPAYKGITLAAATAVALAQLGHNVPF
                     SFNRKEAKDHGEGGSIIGAPLAGRVLIIDDVISAGTSVRESEAMIRAAGAVPAGVLIA
                     LDRQERGQGAMSATQEVSRDHGIPVTAIATLDDVLATLRGRADLRDRIAAIEEYRRQY
                     GA"
     gene            17594..18304
                     /locus_tag="VFV55_03175"
     CDS             17594..18304
                     /locus_tag="VFV55_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4943327.1"
                     /translation="MKILVILAMLAAPAAHAVYKCKDEKGITHVGDTPPAACANVVLY
                     EIAKSGNVIRTIDPTPTGDQVRAKTEEAARRKEAEKAAAEQRRKDIALLATYSSEQDI
                     DTSRDLNLKPIEGRIQSARERTLAVEKRTKELETELEFYKAGKGKGGKSPATVREAPP
                     QLKADLEQVQREKASLAQSITNYGKEMQEVRERYDADKKRWVELKQMNREGKFDLRDS
                     REIEASRKTEPAKQPARK"
     gene            complement(18345..>18878)
                     /gene="gatB"
                     /locus_tag="VFV55_03180"
     CDS             complement(18345..>18878)
                     /gene="gatB"
                     /locus_tag="VFV55_03180"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833187.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX4943328.1"
                     /translation="AMRERFVSGHGLNAADATTLTASKALATYFEEVVRLAGGEPKAC
                     ANWVLGELSALLNRLEIEADASPVAPAALAGLVKRVLDGTVSGKTAKEIFEAMAAGEG
                     EADTLIEKRGLRQISDTGAIEALVDQVLAANAKLVDDYRSGKEKAFNALVGQVMKASQ
                     GKANPAQANAILKRKLG"
BASE COUNT         3177 a         6492 c         6388 g         2821 t
ORIGIN      
        1 gggcgcggct actacaacgg ctagggccat gaccaagtcc ccggcgcccg tcacgcaggc
       61 gatgctcaac gccgcgggcg ggaatgcagg caactggatc cattccaacg gcagctacga
      121 gcagacgcgt ttccacccgg gcaagctgat caacaccggc aacgtcgcga agctgaagcc
      181 cgccttcgtc ttccagacgg cggtgctgga atcgatggaa acggcgcccg tcgtggtgga
      241 cggcgtcatg ttcctgacca cgtccttcaa ccacgtctac gcgatcgacg cggccacggg
      301 cgaggagttc tggcactaca agcacaagct cggtcccatc gtgaccgtgt gctgcggcaa
      361 caacaaccgc ggcgtggcga tcgccggcgg cacgctctac atggggacga tcgacgccaa
      421 gctggtggcg ctggacgcga agaccggcaa gctcctctgg gagacgcaga tcgccgatcc
      481 cgagaagggc tactcggaaa cgatggcgcc cgtggcggtc gacggcaaga tcctcatcgg
      541 caccaacggc ggcgaatacg ggatccgcgg cttcgtgaag gccttcgacg ccagggacgg
      601 caagaacctc tggaccttcc acacgattcc tgagacggga cacgaaggcg tgtgggcggc
      661 cagggatgcg accgggcgcg acatgcaccg cgacatcgcc gcggagaagg cggctctcgc
      721 caaggacagc tcgttctaca aggcgctcgg cggcggcgtg tggatggcgc ccgcggtcga
      781 tctcaagacg cgaacggtgt tcttcgtcgt gggcaacccg tcgccggacc tctatggcgc
      841 gatccgcccg ggcgacaacc tgtacaccga ttccatcgtc gcggtcgacc tcgacaaggg
      901 cacgtacaag tggcacttcc agtacatcgc ccatgacgtg tgggacctcg acgccgtgag
      961 cccgccgatc ctcacgcagg cgaagaatgc cgccggaaag atcgtggatg tcgtcattca
     1021 cggcggcaag accggccaca tctacgtgca cgagcgcgac acgggcaagc tgatccgctt
     1081 ctcggacgcg atggtgccgc aggagaacat gtgggtgctg ccgaccaagc agggcgcgcg
     1141 gatgctgccg ggcgccaacg gcggggtgga atggtcgccg atggccgtcg atccgaaggc
     1201 gcgcgtggcc tacgccgcga acctgcacca gcccatgacc taccacgtcg aggaaacgcc
     1261 gtatccgggc ggcaagctct ggctgggcgg cgccttcaag acgatccccg gcgaggagca
     1321 gtggggcaat ctcgtcgcgg tgaacctcga caacggcaag ctcgtctggc agcacaagac
     1381 gccgcagccc ctgatcggcg gcgtgctcgc gacggccggc ggcctggtct tcaacggcga
     1441 ggccaacggc tggttcaagg cgttcgacgc gaagaacggc aaggagctct ggaaattcaa
     1501 ctgcggggcc ggcgtcaacg cccccgcagt gtcgtacacg gtgaacaaca agcaatacgt
     1561 cgccgtcgcc gccggcggca acaaccagat cgactccaag cggggcaaca gcgtcttcgt
     1621 gttcgcgctg ccctgatcca tcccggaccg atgaccgatc cggtgtctga caccggatcc
     1681 gtcatcgcag gagtccacgc ccatgaagcc actggctgcg gcgctcgctt gcgccgccaa
     1741 cctgatcctc gttttcccgt ccgccgcgca ggatgacccg gcggtccgga aggccgccga
     1801 accctgcttc gcctgccacg ggccggaagg caattccgtg aacccggcgt acccgatcct
     1861 cgccggcgaa acctggcgct acatctacat ccagctcaag gatttcaagg aaggccggcg
     1921 tgccgacccc ctgatgtcgc cgatggccgc caacctgaca cgcgacgaca tgatcgcgct
     1981 cgggaacttc ttcgcggcgc agaagcgcag gcccaacggc ttcaaggccg atggcgcgcg
     2041 cgtggcggcc ggcaagaaga tttccgacgc ggtgctctgc ccgatgtgcc acctgggagg
     2101 attctccggc cagaacgaca tcccgcgagt cgccggccag caccccgact acatccgcaa
     2161 gcagttgcgc gacttcaagg cgaggaagcg gacgaacgac gcgggcaaca tgacgagcgt
     2221 cgccgccaac ctctccgacg acgacatcga gaacctcacg cactacatcg ccaacctgaa
     2281 ctgagcgggc ggcggccccg gcgcgacgcc cccgtaaggc tgtaaccttg ctgtcatccc
     2341 ggccccgtaa gatccaggct ggtcacggtt tcccggcttt gccatgcccg cccacatcct
     2401 cgtcgtcgaa gacgaaccag ccatccagga actgatcgcc ctgaacctcc ggcaggccgg
     2461 gcagcgcgcg gtgctggccg acagcgccga gcaggccatg gagcaaatcc gggaggagct
     2521 gccggacctg gtcctgctcg actggatgct gccggggcag agcggcctgg agcttgcccg
     2581 gcgcctgcgc ggcgacgcgc gcacgcgaaa tctcccgatc atcatgctca cggcacgcgg
     2641 cggcgaggaa gacaagctgc gcggcctgga aaccggcgcc gacgactaca tcaccaagcc
     2701 cttctcggtg aaggagctgc aggcgcgcat caaggcggtg ctgcgccgcc gcgcgccgga
     2761 gatcaccgac gacgtggtcg actacgacgg cctccggctc gaccccgcct cgcaccgcat
     2821 caccggcaag ggccgggaac tgccgatggg gcctaccgag ttccggctgc tgcatttctt
     2881 catgacccac ccggagcgcg tgcacacgcg cacccagctc ctcgaccagg tgtggggcga
     2941 ccacgtgttc atcgaggaga ggacggtcga cgtgcacatc cggcggctgc gctccgcgct
     3001 ggcgaaaagc ggccaccacg ccctgatcca gacgatacgc ggcagcggct accggttttc
     3061 aagcgcgacg aaatgaagga ctggatcggc cgcctcgcct cgatagccgc catcgcgggc
     3121 ctgggggcgc ttctcaccgg ctacatcgcg ggcgcggagt tcgccgcgct cttcctcttc
     3181 gccttcctcg tgatcgtggt ttcgatcggg ctgagcggcc gcgcccgcct cgagcgctgg
     3241 ctcgccgaac cctcggtgga cgacctgccg gaaggggaag ggccctggga cgagatctac
     3301 gcgcgcctgc accggatcct gcgccagcag agcaacaccc gcgcgagcct ctcgcacgcg
     3361 ctgtggcgct tccggcaggc gggcgaggcg atgcccgacg gcgtgatcgt gctcgacggc
     3421 ggcaaccgca tcgaatggat gaatccctcg gccgagaatc acttcggcct cgccttcaag
     3481 gacgatcgcg ggcaggcgat caccaacctc atccgccaac cgtccttcgt gagctacctc
     3541 gacgccaggc gctacggcga gccactcacc ctcaagggag cgagcgaggc ggaccgcatc
     3601 ctttccgtgc agctcgtccc ctatggcgac cgcgagaaac tccttctttc gcgcgacgtc
     3661 acgcgctggg aacggctgga ggccgtgcgg cgggatttca tcgccaacgt ctcgcacgaa
     3721 ctgcgcacgc ccctcacggt gctgaagggt ttcctggaga cgctcaccga cgcgaggcag
     3781 gcggacgaga agctgtaccg ccgctcgctg gagctgatga cggaccaggc cgagcgcatg
     3841 cagcgactcg tcgaggacct cctcatgctc tcgcggctgg aggacacgcg ttaccccctg
     3901 cgcgaggagc ccgtggacgt gccggcgctc ctccagtcgg tgctcgtcga ggcggagagc
     3961 atcaaccagg ggcggcaccg catcgcctcg aagatcgagc cggcgtggct cctcgccagc
     4021 cgcgaggaaa tccgcagcgc catcaccaac ctcctcacca atgccatccg ctacacgccg
     4081 gccggcggtg acattgccgt caccttcgcg atcgagaacg gcgagccgac cctgcgcgtg
     4141 cgcgacaacg gcgagggcat cgcgccggag cacatcccgc gcctcaccga gcgcttctac
     4201 cgcgtcgacc gcagccggtc gcgctccacc ggcggcacgg gactggggct ggcgatcgtg
     4261 aagcacgtgc tgcagcgcca ccaggggcgg ctcgacatcg cgagcgaggt cggggcagga
     4321 agcacgttct cctgcgtctt tcccgccacc cgcatcgcgt cgccatcggg aagccccgcg
     4381 caggacacgc cggtccgggc ggcgtaggga cggcgtgcgc aaaagcgccg ggccgagtta
     4441 tcctatccgc tccgaaagcc gcgagaaccg tcgcgttgac acagaaatcc gcttacaacc
     4501 gcgatgagct gatcgcttgc ggccagggcc gtctgttcag gccggacagc ggccgcctgc
     4561 cgcttccgcc catgctgatg ttcgaccgca tcacccggat caacgacgat ggcggcagcg
     4621 agggcaaggg cgagatcgtc gccgaactcg acgtgaagcc ggacctctgg ttcttcggct
     4681 gccatttcga aagcgatccg gtgatgccgg gttgcctcgg cctcgacgcg ctgtggcagc
     4741 tggtcggctt ctacctggtc tggcgcggca accccggccg cggccgcgcg ctcggatccg
     4801 gggaggtcaa gttcgtcggc caggtcctgc cgacggcgaa actcgtgacc taccggctca
     4861 ccctcaagcg cctgatcgaa cgcaagctgg tcctgggcat cgccgacggc agcgtgctgg
     4921 tcgacggccg cgagatctgc accgccatcg acctcaaggt cgggctgttc acctccacgg
     4981 acgccttctg atgcggcgcg tcgtcatcac cggcctcggc atcgtctcct gcctgggcaa
     5041 cgacaagcag ggcgtgagtg cgtccctgag ggagatgcgc tccggcatca cccatcagcc
     5101 cgagtacgcg acgatggggt tccgcagcca ggtcgccggg gcgccggtca tcgacctcga
     5161 ggccgcgatc gaccgcaagg tacggcggtt catgggccag gcagcggcct acgcctacgt
     5221 gtcgatgaag gacgccattg cggatgccgg actgacgccc gacatggtct ccaacccccg
     5281 caccggcctg atcacgggtt ccggaggggg ttcgccggag aaccaggtcg aggccgccga
     5341 caccgcccgc agcaagggcg tcaagcgcat cggggcgttc atggtgccgc gcacgatgac
     5401 cagcacgacc tcggcctgcc tcgccacgcc cttcgggatc aagggcatca atttcaccat
     5461 cgcctcggcg tgcgcgacca gcgcgcactg catcggcgag agcgcggaga aaatcgcgct
     5521 gggcaagcag gacgtgatgt tcgccggcgg cggcgaggag ctgcactggt cgctttcctg
     5581 cctgttcgac gcgatgggcg cgctgtcgtc cgggcgcaat gacacgccgg ccgaagcgtc
     5641 gcgcgcctac gacgccggcc gcgacggctt cgtgatcgcc ggcggcggcg gcatggtcgt
     5701 gctcgaggag tacgagcacg cgcgcaaacg cggcgccagg atctatgccg aggtcgtcgg
     5761 ctacggcgcg acttcggacg gctacgacat ggtcgcgccc tccggcgagg gcgccgtgcg
     5821 ctgcatgcgc atggcgctcg agaccgtgca gggtccgatc gactacctca acacgcacgg
     5881 cacctccacg ccggtcggcg acaccgccga gctcggcgcg atccgcgagg cgttcgcctc
     5941 cggcggaacc gcccctgcct tttcgtcgac caagtcgctg accggccatt cgctcggcgc
     6001 cgcgggagtg caggaggcga tctactgcct gttgatgatg gagggcgggt tcatcgccgg
     6061 ctccgcgaac gtgaccaaca gggacccggc ggtcggcgac ctgcccctcg tcaccgcgac
     6121 tcgcgatgcg aagctcacca ccgtgatgtc gaactcgttc ggcttcggcg gcaccaacgc
     6181 gacgctggtg ctgcgcaggg tgtagcagcc cgccggcggg cgggccggtt ggcgcgccgc
     6241 agccggcggg ccgtagaatg cccccatgat cggcatcctc ctcgtgtccc acggcgcctt
     6301 cggcgaaagc ctcatccact gcgcgagcca cgtgctgggc aggcgccccc tttacgtgcg
     6361 ccagctgggc gtcaccgtgc acgatgttcc ggaagagatc ctccccaccg cggaggacct
     6421 gatccgcttt ctcgaccagg gccagggcgt gctggtgatg accgacatct atggagccac
     6481 cccgtccaac atcgccgcga agctcctcaa gccggggcgc gtcgagggcc ttgcgggcgt
     6541 gaacctgccg atgctcatcc gcgccctcac ctaccgcgag gagccgctcg agaccgcggt
     6601 ggccaaggcg ctgtccggcg gcacggaagg ggtcatgcgc atgttcccgg aagcctgagg
     6661 acccgcgcac ccatgcagaa gcagaccatc accatcgtga acaagctggg gttgcacgcc
     6721 cgcgcatccg ccaagctcac ccagaccgcc gcgagatacc cctgcggcgt gcacctctcg
     6781 aggaacggca agcgcgtcaa cgcgaagagc atcatgggcg tgatgatgct cgcggcgggc
     6841 atcggcgcca cggtcgagat cgagaccgac ggcccggacg aggaagccgc catggaagcg
     6901 ctggtgaagc tctttgccga caagttcgac gaaggcgagt aggaccgcgc ccgccatggc
     6961 cgccgacggc cctgccccga ccgcccacaa cgtctgcttc accatgcacg gcatcggcgt
     7021 gtccggcggc atcgccatcg ggcacacgca cctcttcctc ggcatgtcca ccgaggtcga
     7081 ccactacgat attgcgtacc acgacgtcgg gcgcgagcag cggcgttacg accgcgccgt
     7141 caaggaagtt cgcgacgagc tgaaggtgct cgccgacagc gtgcgccaca gcgccgcctc
     7201 ggaactcgcg cccttcgtga acgtgcacct gatgctcctc gaggacgtgg ccttctccga
     7261 ggccccgcgc aagatcatcg ccgagcagcg ctgcaatgcc gagtgggccc tccgcgccca
     7321 gctcgacgag ctctccgccc agttcgacga catcgaggac gaatacctgc gcgagcgcga
     7381 ggccgacgtg cgccaggtgg ccgaacgcgt gctcaccgcg ctggcgggct ccaagcggcg
     7441 catcgcgatc gagccgacgc gccaggacac cgacctgatc ctcgtcgcac gcgacctgtc
     7501 gcccgccgac gtgatcctct tccgcgagca cccgttcgcg gcgttcgtca cggacatggg
     7561 cggcgccacc tcgcacacgg cgatcatcgc ccgctccctt gccatccccg cgatcgtggc
     7621 cctgcaccac gcgcgcacga tgatccgcga ggacgagctg gtgatcgtgg acggcacgca
     7681 gggcgtgctc atcgtcaacc ccgaccgggc cgttctcgcc gagtaccgcc tcaagcagaa
     7741 ccaggcgcgg ctcgagaagg acaagctcaa gcgcctgaag tcggctccta cggccacgct
     7801 cgacggcacg gcggtgcagt tgcaggccaa catcgagctg ccctcggacc tggacgccgt
     7861 gcgggcgagc ggtgcggccg gcatcggcct tttccgcagc gaattcctgt tcatgaatcg
     7921 ccgcgagctc ccggacgagg acgagcagta cgaggcctac cgccaggtgg tcgagggcat
     7981 gaaggggcgc ccggtcacga tccgcacgct cgacatcggc gccgacaagg cgctcgacgg
     8041 ccaggagcac cagaccgaga acccggccct ggggctgcgc gccatccgct actgcctcgc
     8101 cgagccgcag atcttcaaca cgcagctgcg cgccatcctg cgcgcctcgc gccacggcaa
     8161 ggcgatgatc ctgatcccga tgctctcctc gtacgcggag ctgcaccagg ccgtggccgc
     8221 cgtgcagcgc gccaaggagg aactgacggt cgagggcgtg aagttcgacc ccctcgtgcc
     8281 ggtgggcggc atgatcgaga ttccggccgc ggccatcgcg ctgccgatgt tcatccgcaa
     8341 gctcgatttc ctgtcgatag gcaccaacga cctcatccag tacacgcttg ccatcgaccg
     8401 cgccgacgac gccgtggcgc atctttacga cccgctgcac cccgcggttc tttcgctcat
     8461 cgcgggcgtg atccgcacgg cgctgcagca cgacaagccg gtggcggtgt gcggcgagat
     8521 ggcgggtgac gtggagctca cccgcctgct tctgggcatg ggcttgcgcc gtttctccat
     8581 gcacccggcg agcctgctcg cggtgaagca gcgcgtgctg tccacgagcc tgccggacat
     8641 cgcggcgctg gtggcgcgcg tgatgaaggc cgacgagccg gagaagctca tctcgctcat
     8701 cgagaagctg aacgcatagc catgctccgc gaggtttcgg ccgtcgcgca ggacatcccg
     8761 gggctcaccc gccggtggtt ccgcgacgag tacttcgacc tctacctgtg ggaggggcac
     8821 ggaggcgaga tcggggccct ccagctctgc tacgaccgct cggacaagga gcgaagcctg
     8881 cgctggtcgg cggaaaaggg attcctgcac gaaggcgtgg accgcccgga gacgaagccg
     8941 ggccgggcca tgacggcgat cctggtcgtg gatggcgcga tgccgctggc cgccgtatcg
     9001 aggaagctgc tggccgccgc gaaggaactg ccggccggga tccgcgattt cctcttcgcg
     9061 cggctcgagg agtacgccag gggcgggcgg ccgcccacgc cgggcgggcg cggggcccct
     9121 cgccacgggg ggcgcgagcg gctagaatga ccgcatcgcg ccgaatttga actcagcttg
     9181 cgggcgcgta ataaatgcgg ctaaagcgag aggcaggtct cccgacgaga acccgacccc
     9241 gcgacagccg gtcgggtttt tttgttcgca cgggacgaaa tgaacgacat tcctccaggc
     9301 tccgtgggcg aggtcgcgcc gcagcgcgcg ggtttcctcg agcccctcat cctcaagggc
     9361 ggcaagacgc tcgcctccta cgagctcgtc tacgagacct acgggacgct caatgcggag
     9421 cggtccaacg ccgttctcgt ctgccacgcg ctctcgggca accaccacgt cgccggctgg
     9481 cacgcggaca gcccgaagaa ggcgggctgg tgggacaaca tggtgggccc cggcaaggcc
     9541 atcgacacgg agcgcttctt cgtggtcggc gtgaacaacc tcggcggctg ccacggttcc
     9601 accggtccga cctccatcga ccgcgccacg agccagccct acggcgccgg attcccgctc
     9661 gtcacggtgg aggactgggt ggcttcgcag gcgcggctca tggaccggct gggggtcgag
     9721 aggctcgcgg tggccatggg cggctcgctg ggcggcatgc aggcgctgca gtggacgctc
     9781 gagttcccgg agcgcatcgc gcacgcgatc gtgatcgccg ccgcgccgaa gctttcgacg
     9841 cagaacatcg ccttcaacga cgtggcgcgc caggcgatcg tctccgaccc cgatttccac
     9901 ggcggcaact tctacgcgat gaagacggtg ccggcgcggg gactcaagct cgcgcgcatg
     9961 ctcgggcaca tcacctatct ctcggaggac ctcctgggcg agaggttcgg gcgcgagatc
    10021 aagggcgacg actacggctt caactacgag gtggagttcg agatcgagtc gtacctgcgc
    10081 taccagggcg acaagttcgc gcaggtgttc gacgccaaca cctacctgat catgaccaag
    10141 gcgctggact acttcgatcc ggcccgcgag accggcggcg acttcgcccg tgcgatggcg
    10201 gcggccaggg ccgggttcct cgtggtctcc ttcgccagcg actggcgctt cccgccgcag
    10261 cgctcgcgcg aaatcgtgaa ggcgctggtg aagggcggaa agaacgtcac ctacgccgag
    10321 atcgacgctc cgcacgggca cgacgcgttc ctcctcgaca atgcgcacta ccaccgcctg
    10381 gtggcggcgt acatggacaa cgtggccgcg gaagtgggcg cgggcaggcg cgcgcggccg
    10441 ctcatcgtcg gcggcacgga gctcgaggag atgaagggct acgccggcaa ggggaggccc
    10501 tcgtgacgcc gcgcttcgac ttcgacctca tctcttcgtg gatcccggaa ggcgcgcgcg
    10561 tgctggacct gggctgcggc gacggcgacc tgctcgcggg gctcgctgcc acgcgcggcg
    10621 tgaagggcta cggcgtggag atcgacccgg agcgcatgct cgcggcggtg ggccatggcg
    10681 tgaacgtgat ccaggtggac ctggaaagcg gcctctacgc cttcgaggac gacgccttcg
    10741 atttcgtgat cctctcgcag acgctgcagg cgatgcgccg ctcggaccac atcctcgccg
    10801 agatgctgcg cgtgggacgc gaggggatcg tcaccttccc caacttcggc tactggaggc
    10861 atcgcctgtc catcctgatg gggcgcatgc cggtgtcgga atcgcttccc taccagtggt
    10921 acgacacgcc caacatccac ctgtgcacgg tgaaggattt cgaggacctg tgcgtcaagg
    10981 tgggcgccga gatcctcgac gagcgcgtgc tgcacgaagg gcaggtcgtg gaggttctcc
    11041 cgaacctgtt cgggagcctg ggcgtcttcc gcttcaagcg gaaatagccg gtcgatcgat
    11101 ggggcggccc gctgcgcgcg gccaccgcgc cctgcgtcac gcgccagcga agggattcat
    11161 cacccgcacg ccgccgtagt cctggccgtg cgaaaggtct tcgctcacga gaacggtggc
    11221 gccgaggcgg cgcgcgccgg caatcacggc ggcatcccag taggcgatgc catggcggtt
    11281 cttcagttgc agcgcatggc gcatgagcgg aaggtcggtg gcgcccaccg ggcggctgtc
    11341 ggcgatttcc tcgatcatgg cgagcgcatc gtcgtggccc agctcgtgcg gcgtgcgcac
    11401 cgcattcacg tagaactcct gtagcacctg gacggtgacg ccccagtctt cgcgagcgat
    11461 ccattcgcgc gcgatgcgcg cctttgcggc gtctcgcggg gctttcgaga acgcgtacag
    11521 cagcacgttg gtgtcgaggc agtgcgtgac ggccacgatc agattcccga cttgcgcagc
    11581 cgttggcgat cgtgggcctg ttcgcgcgtg agccgcttcg tggtgtcgat gccgcggtcg
    11641 ccggcgatgg ccaccaaccg gtcgagcagg ctttgcgccg gacgtggggt cgaatcgtaa
    11701 gcggccgcga cggaatgggc ccgtgccatc tgcggatcgt tcgctgcggc ttccaggatc
    11761 gccatcagtt caccctgcag ggaacgatgg ttgcgctcgg cgcgagcgcg cagcatcgcc
    11821 gccaatgcct cgggcacgtt cttgaccgaa agattgaccg tcatagtggc tccataacag
    11881 ctccagatgg agccattatg gaaccgcctg cccggtcagt caaccggccg gtcgagcgag
    11941 cgcagtgcct cgcgcttcca ccagaggaga accagcccgg gaagtgccac gaggaaggtg
    12001 aggaagaaga acacggtcca cgagaggccg aggaacctgg ggtccgtggc gtagcccgcg
    12061 gcgggaccca cgtacacgcg gccgaaggcg gccagcgcgg aaagcagcgc gtactgcgtg
    12121 gcggtgaagc ggtggtcgca cagcgccatg agcagcgcca cgaaggccgc cgtgcccatg
    12181 ccgccgcaga ggttctcgaa gccgaccgcg aagagcatca gcggccagct cttccccgcc
    12241 gcggccagcg ccatgaacgc gaggttggag acggcctgca ggatgccgaa ggccatgagc
    12301 gcgcggtaca gcccgagccg caccatgagc gcgcccccga agagcgcgcc gaagatcgtc
    12361 gcggcgagac ccatgccctt gttcacgtag cccacgtcgt cgagcgagaa gccggcgccg
    12421 cgcagcagga aggcggtggt gagcgagccc gcgaaggcat cccccagctt gtagaggacg
    12481 atgagcgcga gcagcaccca ggctccgggc cgcgagaaga actcctgcag cggcgcccat
    12541 accgcctcgt cgagcgtgcg cggcggcggc gcgcccgctt gcggctccgg cccccacagc
    12601 gtggccatcg cgccaacaac catcaacgcg gccatgagga ggtaggtgtc ctgccagccc
    12661 agcgccggga tgaatcccga tccgccgaca aggacgaggg cggcggcacc cgaggtgagc
    12721 atcgcgatgc ggtagcccac gaccgtgaag gccgccgcga gcccgcgctg ctccggctgc
    12781 gcgacgtcgg tccggtaggc gtcgaagacg atgtcctgcg aggcggaagt gaaggcgacg
    12841 aagacggcca gcgcggcaag ccaggcgaga ttgcccttcg gcgaggcgaa ggccatggcg
    12901 gcgatgcccg cggccagtgc cagctgggtg aggaggagcc agccgcgccg tcgtcccagg
    12961 aagggcggca cgtagcggtc catcatcggc gcccagagga atttccacgt gtagggcagg
    13021 cccaccagcg tgaagaagcc gatggtggag aggtccacgc cctccaccgt catccacgcc
    13081 tgcagcgtgc cgccggtgag cgccagcggc aggccggacg agaaggcgag gagcaggatg
    13141 gccgcgaggt tgcggttgcg gaagacggcg gaatagtcgt gggacgccaa ggtgccgaaa
    13201 ggataccgga accgcgcttc gccacgaccg tccgcggcta gaatgcagcg ttcaacgacg
    13261 cccgccgacg ggccgacccg gagaccgcca tggccaagat cgtcaggacc aacgaggagt
    13321 ggaagaagct gctcaccccc cagcagttcg aggtgacgag gaagcacggc acggaacgcc
    13381 ccttcgccaa cgagtacaac gactgcaagg tccccggaac gtaccagtgc gtgtgctgcg
    13441 ggcaggacct ctttgcctcc gacgccaagt tcgattccgg caccgggtgg ccgtcgttct
    13501 ggcagccggc cgcgccgggc gcggtgctgg aaaaggacga ctcgagctgg ttcgtgaaac
    13561 gcaccgaggt ggtgtgcgcg aactgcgacg cccacctcgg ccacgtcttc gacgacggcc
    13621 ccaagcctac ggggatgcgc tactgcatga actccgcggc tttgaagctc aagccgcgct
    13681 agggccgttg tcgtagcgcg cctcgcccga aggcgggagc ttgcccagct tttcgaggcg
    13741 cagccggatg gagccctggg tgcgcgcgtg cttgcgcgcc acctccggaa tcgtcaatcc
    13801 cgcgtcgaac gcggccgtga cggcgtggtc ctcgccttcg ctccacggct tgccgaaatt
    13861 ggcgggcagg cgctcgcggc gcttttccct ttccacctcg cgctgcatga ggtccacggc
    13921 ggagaagagc gcgcgcagcg cccgcggctc ggcatagggg ctgccttccg ggtaggactc
    13981 gccggtgacg gggttcacgc cgtccgccag cgctttcacg atgggcaatg cggattcgag
    14041 atgcatgggg cctccggtgg gtggggccgc gtcgacatgc gcggccgcat tccggacaac
    14101 gctcattcgc gcgcgcggtt gacctcgacg tccatcgccg cgttggtcca cagccagtcc
    14161 tcctcgagct gcgcgatcct cgccgcgatc tcgccgtggc gtttcgagcg ttccgcgagc
    14221 tccgcgcgca tcgcctcccc gtaggcctcg cccgaggcga gccacgcgtc gagcgcttcc
    14281 ttctcgggct ggagcttcgc gagttccctt tcgatggtgc cgatcctctt ctcgaagggc
    14341 ttcttcgcgt ccgcaagctt ctgccgcgcc tcggcctgcg cgcgtttctg cgccttgcgg
    14401 tcggcgcccg cgcccgtccc ctcggatttc gtcccgcgcg cgcggtactg ccgcgaccac
    14461 tcccggtagt cgtccaggtt gccgtcgaag ggcgcgacct tcccgtcggc caccagccac
    14521 cactggtcgg tggccgcctc caggaggtgg cggtcgtggg cgacgacgat gagcgtgccc
    14581 tcgaaatcct gcagcgcctg cgccagggcc tcgcgcatct cgatgtcgag gtggttggtg
    14641 ggctcgtcga ggaccagcag gttgggccgc tcccacacga tgagggcgag ggccaggcgc
    14701 gccttctcgc cgccggagag gtcggccacg cgctggaaga cctggtcgcc gcgaaagtcg
    14761 aatcccccga ggaaatcgcg cagctcctgc tcgcgcaccc cggcggccag gcgcgcgagg
    14821 tgccacatga cgctttcctc gaggcgcagc ttttccacct ggtgctgcgc gaaataaccg
    14881 acgcgcaggt ccggcgagcg gatgatcgcg ccggccaccg gtggcaggtc gcccacgatg
    14941 gtcttgagga gcgtcgactt gcccgcgccg ttgggcccca gaagcccgat ggcctcgccg
    15001 aagtacacgc tccactcgat gtccgacacc accgccttgt cgccgtagcc cacgctcgcc
    15061 tccgcgacgc ggaacaggag cttcggcttc acgggcggct cgcggaaggc gaaggtgaac
    15121 ggattgtcca cgtgcgcggc cgcaatcact tccagcttct ccagcgcctt gatgcggctc
    15181 tgcgcctggc gcgccttggt ggccttggcg cggaagcggt cgatgaagga gtgcaggtgc
    15241 gcgatggtgc gctgctgcct ttcgtgggcc gcctgctgct gggcgagccg gtcggcgcgc
    15301 tgcacctcga aggcggagta gtttcccgcg tacgacgtca gtttctgcgc gtcgaagtgc
    15361 acgatcgtgc ccaccacgga atcgaggaac tcgcggtcgt gcgtgatgag gagaagcgcg
    15421 ccgggatagg ccttgagcca gtcctcgagc cacatcaccg cgtcgaggtc gaggtggttc
    15481 gtgggctcgt cgaggagcag caggtcggag cggcacatga gcgcgcgggc gaggttgagg
    15541 cgcatgcgcc agccgccgga gaacatcgcc accgcgctcg cctcggcctc gggcgcgaag
    15601 cccaggcccg acatgagcgc ctgcgcgcgc gaacgcgccg cgtagccgcc gatctcgtcg
    15661 tagcgcccgt gcagcatcgc cgcgcgctcg ccttcctcgt gcgccgcctc cgcctcgcgg
    15721 atcgccgctt ccacctggcg aagctccgtg tcgccgtcca tcacgaagtc gagggcggca
    15781 cggtcggtgt cgtgcagctc ctgcgccacg tgggccagca cccagcgcgc cggcagttcc
    15841 acctcgcccg cgtcctggtg cagctcgccc aggacgagcg cgaacaggct cgacttgccc
    15901 gcgccgttgg cgccaaccag gcccacgcgg tgtccgggaa agacggtgag ggaagcttcc
    15961 ttcagcagga cgcgcgcgcc gcgcgccagc gtgatgttcg agaggcgaag cactacagcg
    16021 tgtacccgta gtcgatggtg agcggcgcgt gatccgagaa ccagcggtcc ttgtagatgc
    16081 gcgcgtactc tgccgtccgc gcgatgccgg gcgtggcgat gtggtagtcg atccgccagc
    16141 ccacgttctt ttcccgggcg cggccgcggt tggaccacca cgtgtattgc tccggcaggg
    16201 gattgagcgt gcggaagacg tccacccagc ctaccgtgtc gaaggcttgc gtgagccagg
    16261 cgcgctcctc cggaagaaac ccggagttct tctggttgga gcgccagttc ttgaggtcga
    16321 tctccttgtg cgcgatgttc cagtcgccgc acaggatgaa ctctcgcccc gaggccctca
    16381 tggccttgag cttcggcatg aagcgcttca tgaacgagaa cttgcgcgcc tgcgcctcct
    16441 cgcccgacga tcccgaggga acgtagatcg agaccacgga gagattgccg tagtcgagct
    16501 gcaggtagcg gccctgggcg tcgatgtccc tgatgccgag ccccagctcg gcgcggtcgg
    16561 gctccttcct cgcgtagatg gccacgccgc tgtagccctt cttctcggcc gggtggaaga
    16621 acgcgtgcag cttccccggg gccaggatct cgcggggaag gtcgggttcc tgcgccttga
    16681 tctcctggag gcagaccagg tcggccttct ggcgcttcat ccagtcgagc aggcccttgg
    16741 aggcggccga ccggatgccg ttggtgttca gggtgacgat gcgcacggaa gacctcgttt
    16801 cgggacgcgc tttgggggaa aatagaacgc attctaatgg tcccggtccc catggacgcc
    16861 accgcccccg ccgccggaca ggatttccgc cgccagttcg tcgagttcgc catccgggcg
    16921 ggagtgcttc gcttcggtga attcacgacc aaggcggggc ggctgtcccc gtacttcttc
    16981 aacgccgggc tcttccacga cggggcaagt ctcggggagc tttcgcggta ctacgcccgg
    17041 gccgcgctcg cctcggggat cgccttcgac ctcctcttcg gacccgccta caagggcatc
    17101 acgctggccg cggccacggc cgtggcgctt gcccaattgg gtcacaatgt gccgttttcc
    17161 ttcaatcgca aggaagccaa ggaccacggg gagggcggaa gcatcatcgg cgcgcccctc
    17221 gcgggccgcg tgctgatcat cgacgacgtg atttcggcgg ggacctcggt gcgcgaatcg
    17281 gaggcgatga tccgggcggc cggggccgtg ccggctggcg tgctaattgc gttggacagg
    17341 caggaacggg ggcagggagc gatgtccgcc acccaggaag tcagccgcga ccatggcatt
    17401 cccgttacgg ctattgcaac gctggacgac gtgctcgcca ccctgcgggg gcgggccgac
    17461 ttgcgtgatc ggatcgccgc catcgaggag tatcggcggc agtatggggc atgacccccg
    17521 aatcggcttg gtggtgcacg ggcgcgagag atgcccggca cgggcgcaaa gtaaaggtgg
    17581 cagcgtgggc gcaatgaaga tactggtcat cctggcgatg ctcgcggccc ccgcggcgca
    17641 tgccgtctac aagtgcaagg acgagaaggg catcacccac gtcggggata cgcccccggc
    17701 cgcctgcgcc aacgtcgtcc tgtacgagat cgcgaagtcg ggaaacgtca tccggacgat
    17761 cgaccccacg cccacggggg accaggtcag ggcgaagacg gaagaagctg cgcgccgcaa
    17821 ggaggccgaa aaggcagccg ccgagcagcg ccgcaaggac atcgcgctgc tggccaccta
    17881 cagttcggaa caggacatcg acacctctcg cgacctgaac ctgaagccga tcgagggccg
    17941 catccagagt gcacgggaac ggacgctggc cgtcgagaag cgcacgaagg agctggaaac
    18001 cgaactggag ttctacaagg ccggcaaggg caagggtggc aagtctcccg ccacggtccg
    18061 cgaggccccg ccgcagctca aggccgacct ggagcaggtc cagcgcgaga aggcgtcgct
    18121 cgcccagtcg atcacgaact acgggaagga aatgcaggaa gtgcgcgagc gctacgacgc
    18181 cgacaagaag cgctgggtgg agttgaagca gatgaaccgc gagggcaagt tcgacctgcg
    18241 cgactccagg gagatcgagg cgtcccggaa gaccgagccc gcgaagcagc ccgcgaggaa
    18301 atagggcacg gcacccgtgc cggccgcctg gcttcccgcg ccgttcagcc gagtttcctc
    18361 ttcaggatcg cgttcgcctg cgcggggttc gcctttccct gcgaagcctt catcacctgt
    18421 cccaccagcg cgttgaaggc cttttccttg ccggaccggt agtcgtcgac cagcttcgcg
    18481 ttggcggcca gtacctggtc cacgagggcc tcgatggcgc cggtgtcgga gatctgccgc
    18541 agcccgcgct tctcgatgag ggtgtcggcc tcgccctcgc ccgcggccat cgcctcgaag
    18601 atctccttcg cggtctttcc cgacaccgtg ccgtcgagga cgcgcttcac cagccccgca
    18661 agcgcggccg gcgcgacggg gctcgcgtcg gcctcgatct ccagccggtt cagcagcgcc
    18721 gagagctccc cgagcaccca gttggcgcag gccttcggct cgccccccgc caggcgaacg
    18781 acttcctcga aataggtcgc cagcgccttc gacgcggtga gcgtggtcgc atcggcggcg
    18841 ttgagcccgt ggccggacac gaagcgctcg cgcatcgc
//
LOCUS       DASUEV010000099         6540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1396346, whole genome shotgun sequence.
ACCESSION   DASUEV010000099 DASUEV010000000
VERSION     DASUEV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396346"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFV55_03185"
     CDS             complement(<1..269)
                     /locus_tag="VFV55_03185"
                     /inference="COORDINATES: protein motif:HMM:NF027966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4943329.1"
                     /translation="MVTDRRHFLRRALAMAAAGFLAPVPARATTRAKFADFPFALGVA
                     SGSPTATGFVLWTRLAPDPLGGGGLGPEDIDVGWEVAHDEKFSRIV"
     gene            367..756
                     /locus_tag="VFV55_03190"
     CDS             367..756
                     /locus_tag="VFV55_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4943330.1"
                     /translation="MKDTVKTMNAIAWFEIPAANFDRAVKFYEKVLGGPIKREKMGDN
                     DMGVFPHVGETSVAGCIWRGEGYRPSPDGTAVYLHAEPDLAAALARVGSAGGRVVVPK
                     TALPPGMGHYAQFIDSEGNRVGLYSME"
     gene            complement(794..1321)
                     /locus_tag="VFV55_03195"
     CDS             complement(794..1321)
                     /locus_tag="VFV55_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX4943331.1"
                     /translation="MVAILNRLPSRAVFAAIFMACASLIGFGLVLQHSVGLEPCPMCI
                     LQRYAFVTIGMVALIAAIHGPVGVGVRVYSFLIGAFAIAGGSVSVRQSLLQRFPAPEA
                     ANCMAGDLDYLLGNFPLAQVLPKIFAGTGDCAKVQWSLFGLSIAEWALVWFVLLVTVA
                     VWLAFFRKRRAPAIA"
     gene            complement(1481..1981)
                     /locus_tag="VFV55_03200"
     CDS             complement(1481..1981)
                     /locus_tag="VFV55_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4943332.1"
                     /translation="MKRILIPVDGSATGLLAVKAVLADRSGNVERIELLNVQPRLSRH
                     ASRYIPRRSRDGWREERAHKALEPARRLVESAGIACRTHTGIGVTAQVVADTARLLGV
                     NEIVLGATQRGPVGRLLANSLSSRLLAAASVPVRVIAAAPAPAFERYAAPVGIGIALL
                     AFAAQD"
     gene            complement(1978..2709)
                     /locus_tag="VFV55_03205"
     CDS             complement(1978..2709)
                     /locus_tag="VFV55_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4943333.1"
                     /translation="MELFSPAFLSALAAIIVIDLALAGDNAIVIALAARNLPPNLQRR
                     AIVWGTVGAIAVRVVMTLAVVSLLGIPGLMAAGGAILVWIAIKLLTPGSGESHEIKAA
                     ATFWGAMQTIVIADAAMGLDNVLAVAGAAHGSFALVVIGLLISIPIVVWGSTLILGWV
                     GRFPWIVQVGVAVLAWTSAKMIVSEPLFADFFSAHDAARLFTYALVFAIVFGAGSIAR
                     RRRRAVVHGPLESQQPFANPQEVTP"
     gene            3132..3470
                     /locus_tag="VFV55_03210"
     CDS             3132..3470
                     /locus_tag="VFV55_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4943334.1"
                     /translation="MTRSRMRRYFRHGFLPQIVAFEACVRHGSVTRAAEELSLAQPTV
                     SCLVRKLSDTLGERLLETRSRRVEPTPAGRELLGLYGEIFAAFHRFDERCAPIAIEVP
                     RFPANVSSRR"
     gene            3490..3915
                     /locus_tag="VFV55_03215"
     CDS             3490..3915
                     /locus_tag="VFV55_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX4943335.1"
                     /translation="MPLALPSKKTLTLEAAKLVAAAGEATAVKNGWKVVIAVVDDGGH
                     AIYLQRLDGTQSSSVGTALGKARTAVGFKRPTRLLEEMVNNGRYSFLSVAGEPVLLQG
                     GLPIEVDGQLVGAVGVSGVKASDDEIVAQAGIDALRAAL"
     gene            3975..5321
                     /locus_tag="VFV55_03220"
     CDS             3975..5321
                     /locus_tag="VFV55_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX4943336.1"
                     /translation="MEPHALGLRALHAAFKEGRFTVPDYVRALNDRIAALEPRIQAFE
                     WFEPARAMAEAEERSGGILKDLPLYGVPVGVKDIIATRGIPTRMGSRIFANHVPGRSA
                     GLVRRLEALGGLVMAKTVTTEFAFRHPGKTHNPWNPAHTPGGSSSGSAASVAAGFVPV
                     AIGTQTLGSVIRPAAFCGIVGYKPSFGAISRDGVFPFSRTLDHVGVFARSVEDAAWFG
                     SCLMGLDQRDEATGIRAGMRSLSVPLAPLGHAPRLAVVKTPKWSLASEAQRANFTEAV
                     ANLKDAGALVREVPLPRAFNEAWNVVGAIMDYEASRGFASLESRHRLQLSAALTELLD
                     RGKRITPEQHAKNLARRDEYRRWLDGLYDGCDAIVTIPAAGEAPEGLASTGDATFCSL
                     WSMAGLPAITMPSGRGPRGLPLGLQVVGRYREDERALQVAAWCETVLGVSLGLAGG"
     gene            complement(5237..5944)
                     /locus_tag="VFV55_03225"
     CDS             complement(5237..5944)
                     /locus_tag="VFV55_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943337.1"
                     /translation="MNGLIVATIAIGLFVDRHGGAHGQLAVNVLAWAVFARLWFVSAP
                     AQRSALLACLVYATAGEVFLSLVWGLYHYRLGNIPLFVPPGHVLLFFLGTQLAARLAD
                     GGEWWIAALALPIVAAFAWTGRDTLGPILFGLFLACMILSRSRKLYATMLVLSLAMEL
                     WGTWLGNWAWRREVPWLALVTDNPPLAAGAFYAALDLLVVSTVGARRLQPPASPRLTP
                     STVSHQAATCSARSSSR"
     gene            5960..6379
                     /locus_tag="VFV55_03230"
     CDS             5960..6379
                     /locus_tag="VFV55_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEX4943338.1"
                     /translation="MTRSRRPVRVHDSRAVRIALIAAGFLSVGLGIAGLVLPVLPTTP
                     FMLLAAACFARSSPRFHRWLLHHRLFGPIVSEWERHRAIPWKTKLWAIALMSGTLAVS
                     ITFFVKPPWLKAALAAFGVGLAVWLYRVPSRDRPRPK"
BASE COUNT         1085 a         2165 c         2317 g          973 t
ORIGIN      
        1 acgatgcgcg agaacttctc gtcgtgtgcg acctcccatc ccacgtcgat gtcttcgggg
       61 ccgagcccgc ccccgcccag gggatccgga gcaagtcggg tccagaggac gaatccggta
      121 gccgtcggcg acccggaggc cacgccgagg gcaaagggaa agtcggcgaa cttcgcccgc
      181 gtcgtcgccc gggccgggac aggcgcgaga aatcctgctg ccgccatggc gagcgcgcgg
      241 cgaagaaaat ggcggcggtc ggtaaccatc ggcaacatta tcgcggaacg ctgctgaggc
      301 catgtcgtcg aaaaggggcg gcaccctttc acccgtgcgc gttgcacgac atcccgagga
      361 ggacccatga aagataccgt gaagacgatg aacgcgatcg cctggttcga gatcccggcc
      421 gcgaatttcg atcgcgccgt gaagttctac gaaaaggttc tcggcggccc catcaagcgc
      481 gagaagatgg gcgacaacga catgggcgtc ttcccccatg tgggcgaaac ctccgtggcc
      541 ggctgcatct ggcgcggcga gggataccgg ccctcgccgg acggcacggc ggtgtacctc
      601 cacgccgagc ccgacctcgc tgcggcgttg gcccgtgtcg ggtcggccgg cggccgggtg
      661 gtcgtaccca agaccgcgct gccgccgggc atgggccact acgcgcagtt catcgacagc
      721 gagggcaatc gggtgggact ctattcgatg gaatgagtcc gggaagtccg gcgcgggcct
      781 ggctccgcgc cggtcaggcg atggcgggcg cgcgtcgctt gcggaagaag gcgagccaga
      841 cggcgacggt caccagcaac acgaaccaca ccagcgccca ttccgcgatc gagagcccga
      901 aaaggctcca ctggaccttg gcgcaatcgc cggtgcccgc gaagatcttg ggcaggacct
      961 gcgccagcgg gaagttgccg aggaggtagt ccaggtcgcc ggccatgcag ttcgcggcct
     1021 ccggcgcggg gaagcgctgc agcagcgact ggcgcacgga gacgctgccg ccggcgatgg
     1081 cgaaggcccc gatcaggaac gagtagacgc gaacgccgac gcccaccggg ccatggatgg
     1141 cggcgatgag ggcgaccatg ccgatcgtca cgaaggcata gcgctgcagg atgcacatcg
     1201 ggcacggctc gagcccgacc gaatgctgga gcacgaggcc gaagccgatg agcgacgcgc
     1261 aggccatgaa gatggctgcg aaaacggcac gggaaggaag gcggttcagg atggcgacca
     1321 tgggcgatag gatgctcgat tttcccacag gttcccgccc tgcacgacga tccgcgtcaa
     1381 ttccgcaaga cgcgcaccaa aacagcaaca aaaacaataa gttgcaattc cggatttgcc
     1441 aaaaggggcc gcggccgaat gggccgcggc ttcccttcgg ctagtcctgc gcggcgaagg
     1501 cgagtaacgc gattccgatg ccgaccggcg ccgcatatcg ctcgaaggcg ggcgccgggg
     1561 cggccgcgat gacccggacg ggcacgctgg ccgcggcaag cagccgggaa ctcagcgaat
     1621 tggcgagcag tcggccgacc ggaccgcgct gcgtcgcgcc gagcacgatc tcgttcacgc
     1681 cgagaagccg cgcggtatcg gcgacaacct gcgcggtcac gccgatgccg gtgtgcgtgc
     1741 ggcacgcgat tccggccgat tcgacgagcc ggcgcgcggg ctcgagcgcc ttgtgcgccc
     1801 tctcctcgcg ccacccgtcg cggctgcgcc tcgggatgta ccgcgaggcg tgccgcgaga
     1861 ggcgtggctg gacgttcagg agttcgattc gctcgacgtt gccggatcga tccgcgagaa
     1921 ccgcctttac ggcgagcagg cccgtcgcgc tgccgtcgac ggggatcagg atacgtttca
     1981 tggcgtcacc tcctgggggt tggcgaacgg ttgttgcgac tcgagcggcc cgtggaccac
     2041 tgcgcgacgg cgacgacggg caatggagcc ggcaccgaag acgatggcga agacgagcgc
     2101 gtacgtgaac agccgcgcag cgtcgtgagc ggaaaagaag tcggcgaaga gaggctccga
     2161 gacgatcatc ttcgcgctgg tccaggccag gacggccacg ccgacctgca cgatccacgg
     2221 gaagcgcccg acccacccga ggatgagcgt cgaaccccag acgacgatcg ggatgctgat
     2281 gagcagcccg atcaccacga gggcgaagga cccgtgcgcc gcgcctgcca ccgcgaggac
     2341 gttgtcgagg cccatggccg cgtcggcgat cacgatggtc tgcatggcac cccagaacgt
     2401 ggccgcggcc ttgatctcgt gcgattcgcc gctgcccggc gtgagcagct tgatggcgat
     2461 ccagacgaga atcgcgcccc ctgccgccat gaggccgggg atgcctagca gcgagacgac
     2521 ggccagcgtc atcacgacgc gcaccgcgat cgctccgacg gttccccaga cgatcgccct
     2581 tcgctgcaag ttgggcggga gattgcgggc ggcgagcgcg atcacgatcg cgttgtcgcc
     2641 ggcgagcgcg aggtcgatca cgatgatggc ggcaagcgcc gagaggaagg ccggtgagaa
     2701 aagctccatc gctgtctcct gggcgaagcg ttgtcgttcg atatgcggca attattgaag
     2761 gcggtcagcg ccggcggatg gagattcgca atcgcgccat tgcaattgcg gatgcattgc
     2821 gattcgccat gcgtgccgga ttgcgaaacc gcgccggacc cgatataaag gggctgcgct
     2881 tcgcccaggc ggagcgtgcc ctccaaaggg gagtagcttt tacggcggag tcgtcaatac
     2941 gggccctcgg gttccggctc cggcggcaag cgagagcgcc agtcgcccgt ttgttagcga
     3001 gacctttgcc agatcgtggc aaaggtcttt tcgcttctgc ggcctttgtc ccgacccggg
     3061 caaaggccgc gtcgtttccg gtgcccggta acgacgcggg ttggctaacg aacttccgtc
     3121 aggagggatt catgacccgc tcccgcatgc gacgttattt ccgccacggc ttcctgcccc
     3181 agatcgtggc cttcgaggcc tgcgtgcgcc acggcagtgt cacgcgcgcg gccgaggagc
     3241 tttccctcgc gcagcccacg gtgtcctgcc tggtgcgcaa gctttccgat acgctcgggg
     3301 agcggctcct cgaaacacgc tcgcgccgag tggagcccac gcccgcgggg cgcgagcttc
     3361 tcggcctgta tggggagatc ttcgcggcct tccaccgctt cgacgagcgc tgcgccccca
     3421 tcgcgatcga ggtgccaagg ttcccggcca atgtatcatc gcgccgttga cctcgttccg
     3481 gagccggcca tgccactcgc tcttccttcg aaaaagacgc tcaccctgga ggccgcgaag
     3541 ctcgttgcgg ctgccggcga ggcgacggcc gtgaagaacg gctggaaagt agtgatcgcg
     3601 gtggtggacg atggcggaca cgccatctac ctgcagcgcc tcgatggaac gcaatcctcc
     3661 agcgtgggga cggcactcgg caaggcgagg actgccgtcg gattcaagcg accgacccgc
     3721 ctgctcgagg aaatggtgaa caacgggcgc tattcgttcc tttcggtcgc cggcgagccg
     3781 gtgctgctgc agggcgggct gccgatcgag gtcgacggcc agctcgtggg ggccgtgggg
     3841 gtgtcgggcg tcaaggcgag cgacgacgag atcgtcgccc aggccgggat cgacgccctg
     3901 cgcgccgctc tctgagcggg tagggccggc gctccgggtt ggccccgagg gggttcaccc
     3961 ggcagaatag cgggatggaa ccccacgccc tcggccttcg cgcccttcac gcggccttca
     4021 aggaaggtcg cttcacggtc ccggactatg tccgggctct caacgaccgg atcgccgccc
     4081 tcgagccgcg catccaggcc ttcgagtggt tcgagcccgc ccgcgccatg gccgaggccg
     4141 aggaacgctc cggcggcatc ctgaaggacc tgcccctcta cggcgttccc gtgggcgtga
     4201 aggacatcat cgccacgcgc ggcattccca cgcgcatggg ctcgcgcatc ttcgcaaacc
     4261 atgtgccggg gcgttcggcc ggcctggtgc ggcgtctcga ggcgctcggt ggcctggtga
     4321 tggccaagac ggtcaccacc gaattcgcct tccgccaccc gggcaagacg cacaacccgt
     4381 ggaatcctgc ccacacgccc ggcggctcgt cgagcgggtc cgcggcgtcg gtggcagcgg
     4441 gattcgtgcc cgtcgcgatc ggcacgcaga cgctgggatc ggtgatccgg cccgcggcgt
     4501 tctgcggcat cgtgggctac aagccgagct tcggagccat ctcgcgcgac ggcgtctttc
     4561 cgttcagcag gacgctggat catgtcggcg ttttcgcccg ctcggtggag gacgcggcct
     4621 ggttcgggtc ctgcctcatg ggcctcgacc agcgtgacga ggccaccggc atccgcgccg
     4681 gcatgcgctc gctctccgtg ccgctcgcgc cgctggggca cgcgcctcgc ctcgcggtgg
     4741 tgaagacgcc gaagtggtcc ctcgcgtccg aggcgcagcg ggccaacttc accgaggccg
     4801 tggccaacct caaggacgcc ggtgccctgg tgcgcgaggt gccgttgccg cgcgctttca
     4861 acgaggcctg gaacgtggtc ggggcgatca tggactacga ggcgtcgcgc ggcttcgcct
     4921 cgctcgaatc gcgccaccgc ctgcagcttt cggccgcgct caccgagctg ctggatcgcg
     4981 gcaagcgcat cacgccggag cagcacgcga agaacctggc gcgccgcgac gagtaccgcc
     5041 gctggctgga cgggctttac gacggatgcg atgccatcgt cacgatcccc gcggcgggcg
     5101 aggcgcccga ggggctcgcc agcaccggcg acgcgacctt ctgctcgctg tggtcgatgg
     5161 ccgggctccc ggcgatcacg atgccttcgg ggcgtggccc gcgcggcctg ccgctggggc
     5221 tgcaggtcgt ggggcgctac cgggaggacg agcgcgcgct gcaggtggcg gcctggtgcg
     5281 agaccgtgct gggcgtgagc ctggggctcg cgggcggttg aaggcgccgc gcgccgacgg
     5341 tgctcaccac gagcaggtcg agcgcggcgt agaacgcccc ggccgcgagc ggcgggttgt
     5401 cggtgacgag cgcgagccag ggcacctcgc ggcgccaggc ccagttgccg agccaggttc
     5461 cccacaactc catcgcgagg gacagcacca gcatcgtcgc gtacagcttg cgcgagcgcg
     5521 acaggatcat gcacgcgagg aataggccga acaggatcgg cccgagcgtg tcgcggccgg
     5581 tccacgcgaa agctgcgacg atcggcaacg ccagcgcggc gatccaccac tccccgccat
     5641 ccgcgagccg tgcggcgagc tgcgtgccga ggaagaagag caggacgtga cccggcggca
     5701 cgaagagcgg gatgttgccg aggcggtagt ggtacaggcc ccacaccagc gagaggaaca
     5761 cttcgccggc ggtggcgtag acgaggcagg cgaggagggc ggagcgttgc gcgggagcgg
     5821 acacgaacca gagccgggcg aagacggccc acgcaaggac gttcacggcg agctggccgt
     5881 gggcgccgcc gtgccggtcc acgaaaagtc cgatcgcgat ggtcgcgacg atgagcccgt
     5941 tcatggcgcg cattctagcg tgacccgttc ccggcgcccg gtgcgggtgc acgattcgcg
     6001 cgcggttcgc atcgcgctca tcgccgcggg tttcctcagt gtggggctgg gtatcgcggg
     6061 cctcgtcctt ccggtgctgc cgacgacgcc gttcatgctc ctggccgcgg cgtgtttcgc
     6121 acgttcctcg ccgcgattcc accgctggct cctgcatcac aggttgttcg ggcccatcgt
     6181 gtcggaatgg gagcggcacc gcgcgatccc gtggaaaacg aagctctggg ccatcgcgct
     6241 gatgtcgggg acgctcgccg tctccatcac gttcttcgtg aagccgccct ggctcaaggc
     6301 ggcgctggcg gcattcggcg tcggcctcgc cgtgtggcta taccgcgtgc cttcccggga
     6361 tcgtccgcga ccgaagtaga cgggcagggc atgccgatcg caatcctggg cacgcgggtc
     6421 gaggaggcgg tggcggtgga agtcgatcgc aatcctgggc acgcaagcgt gaccgtcgga
     6481 tgggtgcccg gaaagtcgag tccagccaaa cacgtctggc ctcgactttc cacccatcta
//
LOCUS       DASUEV010000100        11991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_602216, whole genome shotgun sequence.
ACCESSION   DASUEV010000100 DASUEV010000000
VERSION     DASUEV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11991
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602216"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFV55_03235"
     CDS             complement(<1..620)
                     /locus_tag="VFV55_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4943339.1"
                     /translation="MAAVLKHQTLPPVKLIRNVPKEEYYVPGHRTCAGCGPALQYKLV
                     AKAAGPNTIFLGPTGCMYVANASYLCTPYMVPWMHTQITNGGAVASGVEAAYKVLLRK
                     GKRTGEFPNIIVMAGDGGTVDIGLQAMSAMMYRGHDALFICYDNEAYTNTGIQTSPTT
                     PYGANTTFTPPGPAIPEGKKLFPKDPPQLVIGGHPAVKYVATASVA"
     gene            complement(637..1863)
                     /locus_tag="VFV55_03240"
     CDS             complement(637..1863)
                     /locus_tag="VFV55_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4943340.1"
                     /translation="MAKEMMLSGCASFAQAAKMAQVEVICSYPIRPYTAIMMELAKMV
                     ANGELDAEFVHGEGEHAQLAVVMGASAAGARALTGSSGVGVTYAFENYSPIAGGRLPL
                     QMAIADRALDPPGDFGSEHTDAMSCRDQGWLMGWAETPQEAFDNCLINYRVGEDHRVL
                     LPQYVCQDGYFVSHIPGLVKIPDQAQVDDFLPPYNLPHPLDPKRPVSHGPQIRPDQGT
                     VMDMQRAQAMLDTPAVIKEATDDYNRIFGRNYSPFVEEFMTEDADIGFFIMGAHTMTA
                     RHAVKHLRAQGVKAGLVKLRWVRPWPTQEVAQALAKFKAVGVVETSTSYGGAMRGGNL
                     LHEVRASLYELEKRPLTTSFMAGLGGEMVPLNDFYYMAGVLAKALKEGRTRQTVYWVG
                     FEAEDAKVKQEETATA"
     gene            complement(1876..2202)
                     /locus_tag="VFV55_03245"
     CDS             complement(1876..2202)
                     /locus_tag="VFV55_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943341.1"
                     /translation="MFNIPSDKQVPLGCVTPAPVGEQPMMITGNWREYRPVLEAEKCD
                     LCMNCIVFCPDACFSLDDAGEQVVLNEKYCKGCNICINECAPGALTAAYELDFDDGVV
                     RLDKPF"
     gene            complement(2217..2831)
                     /locus_tag="VFV55_03250"
     CDS             complement(2217..2831)
                     /locus_tag="VFV55_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX4943342.1"
                     /translation="MASKVHQITVWARGVVQDKEGRDIANGLARAADKEGKFTQAFDN
                     YVDLPDRINVPLRKYARVSDEEIEERYEYENAHPEVVIVADATIVKGIDILRGMEKGG
                     TLVVATDRKPEDILKFIPNRNLLDQIVCVDAKTICGDQTIDFSGSEGGVDSLGIGQGI
                     AAPILGALARGTTGLVKLENLDAVVKNKDALHKGHDQAVVMSLH"
     gene            3000..3944
                     /locus_tag="VFV55_03255"
     CDS             3000..3944
                     /locus_tag="VFV55_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4943343.1"
                     /translation="MKNATFRQLEVFEAVARHGSYSRAAAELRMSQPAVSIHVRQLET
                     HAGVPLFEQFGKRIHLTPAGEEVLRYSRAIIQQRREVDGALAALKGIRGGRLNIAVIS
                     AGDYFFPGLLAEFCRRHEGVTVRLAVNNREEILRQLTEDTTDLTVLLRPPEDSDMVAE
                     AFAPQPHVIIAAPDHQLVRRRQIPLRELAQEAFIVREQGSDTRLAMDELLAESGVRFN
                     ITMEIRSTETIKQAVIAGMGISFLSAHTLGRELEDGQLAVLDVEGFPVMREWHIVHHR
                     NKRLPPVAVAFKDFLLQEGAARIEQLLGRAAAKRKPTA"
     gene            4036..5010
                     /locus_tag="VFV55_03260"
     CDS             4036..5010
                     /locus_tag="VFV55_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX4943344.1"
                     /translation="MPHAIRFHKTGGPEVLQWEDVPVADPAPGEARIRLKAIGLNYID
                     TYHRSGLYPLPLPSPIGREGAGVVEAVGAGVTDLKPGDRVAWTTGPLGTYAEVKNHPA
                     ERLVKLPDAISFEQAACMMLQGLTVHYLLRRTYPVKAGETILFHAAAGGVGLIACQWA
                     KALGATLIGTVGSEEKAKLALANGCAHAIIYTKEDFLARVKEITGGKGVPVVYDSIGK
                     DTFMKSLDCLQPRGLMVTFGNASGPVEPFNLGILAQKGSLYVTRPTLDTHVGKREELL
                     AATKELFDHVLAGRIRIAPRQSYALKDAAQAHRDLEARKTTGSTILVP"
     gene            5051..6010
                     /locus_tag="VFV55_03265"
     CDS             5051..6010
                     /locus_tag="VFV55_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX4943345.1"
                     /translation="MALLLKERDVEQLLTMPLAIDLVERVHREYSAGNAIDIPRERTR
                     LPKAALHILQGAVPSAGVFGYKAYTSSREGIRFIVYAFNAERGNLDAIVEANYLGMIR
                     TGAAGGVAAKWLARPDAKVAGVFGSGWQAQGQIEALAAVRALDRVKVYSRKAEKLAAF
                     CKRMGDRLGLDVVPAGTPEETVRGSDVVVTITTSATPVFDGNWIEPGQHISAAGSNSL
                     LRQEIDETAVRRCSPIVVDSRPTAVKEAGDLLPALEKGRLHAGQLAEIGEVIDGTRAG
                     RTMPEQVTLFESQGMAIQDLAIVAELAKMARARGLGTEIDVGT"
     gene            6095..8707
                     /locus_tag="VFV55_03270"
     CDS             6095..8707
                     /locus_tag="VFV55_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4943346.1"
                     /translation="MKSQFWKADWFFGLVIAIGVLVFNSASNLIPGLERWAYDLGVKM
                     TSKNPSDRIAVVAIDEQSIANIGRWPWPREVQAKLIDQLSAARARVIGNTVFFFEPQV
                     DPGLAYVEKMLAIYDRAYPGAAPQEAPGVAAIGAVTPAAQVAQPAGEVAEIGRLLREA
                     SVALNNDIRLAASVKKAGNVVVPMVFDEMTNAPPPGRPDKPLPEYVARNAIGSASQAG
                     GSLIHGVSLIAPIEQIGANVVAMGHLNATPDPADGAIRSEPLVIDYYGQQFPSLALMI
                     AAKSLNLGAKDIKVNPGESVALGNKVVRTDEQAKMFTYFYKDRDGRAAFPVDSFFDVY
                     TGKIPAAKYADKIVLIGAVAAGIGTNQVTPISAQMNPVTTLAHSVSSLLQEHFFVAPT
                     WGIYATIGAYLLVAAYLVVLLPRLGAGMGAILTALLFVALFGAHLGLMTGAGLWLQLM
                     LPATLLVIGHLLLTTKRFLVTERGKERSDIEGAESNRMLGLAFQQQGQLDMAFDKFRK
                     CPYDEQLGENLYALALDFERRRQFNKAESVFGHIHRQDPKFKDVAERMQRAKTMSETI
                     ILGGSSAARSSAATMVLQGGQTEKPMLGRYQVEKELGKGAMGVVYLGKDPKIGRTVAI
                     KTMALASEFEGDELQEAKERFFREAETAGRLTHPNVVTIYDAGEEHDLAYIAMEFLRG
                     KDLVPYTKPDTLLAPDKVLSIVERVADALGYAHSMGVVHRDIKPANIMYEPESDTAKV
                     TDFGIARITDSSRTKTGMVLGTPSYMSPEQLAGKKIDGRSDLFSLGVTLFQLLSGRLP
                     FEGESMTQLMFAIASNPHPPIREINPQLAPWIDAIIDKALAKDVNVRYQTGAEFAEAI
                     RQARKGGPSVNVSL"
     gene            8694..9266
                     /locus_tag="VFV55_03275"
     CDS             8694..9266
                     /locus_tag="VFV55_03275"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEX4943347.1"
                     /translation="MSLSEPKAKREPLHRRTIEIVGYKREDGLFDIEGRLLDRKDVEF
                     PVGGRRKLPGEPVHEMWLRITVDRELRIVDAEAATDAMPYVGHCDRITPDYAKLIGLS
                     IGPGYLRKVKELLGGIHGCTHITELAGSLATAAFQTFAGQGLAPPDRKPPQLDRCHAL
                     DVHAEAVRLFYPRWYRGTTPVGEPGPTENH"
     gene            9364..10527
                     /gene="sucC"
                     /locus_tag="VFV55_03280"
     CDS             9364..10527
                     /gene="sucC"
                     /locus_tag="VFV55_03280"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX4943348.1"
                     /translation="MNIHEYQGKEIFRKFGVPTPRGIPAFSVDEAVKAAETLGGKVWV
                     VKAQIHAGGRGKGGGVKVAKSPAEVKDLAGKILGMTLVTHQTGPEGRKVRRLLIEEGA
                     DIRKELYVALVVDRVTQRATMMASSEGGMDIEEVAAHTPEKIHRVAIDPRTGLKDAEA
                     EDIARKIGVPEGSIAQARDLLKNLYRVFDECDCSLAEVNPLIVTGEGKVVALDAKLNF
                     DSNALFRHPEIVAYRDLDEEDPAEVEASKFDLSYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGGNPANFLDVGGGATTEKVTEAFKIMLARPGLKAILVNIFGGIMKCDTIANGVVA
                     AAKEVHLSVPLVVRLEGTNVDIGKKILADSGLPIISASDMGDAAAKVVAAAKG"
     gene            10538..11413
                     /gene="sucD"
                     /locus_tag="VFV55_03285"
     CDS             10538..11413
                     /gene="sucD"
                     /locus_tag="VFV55_03285"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX4943349.1"
                     /translation="MSILINKDTKVITQGITGKTGQFHTKMCRDYANGKNCFVAGVNP
                     KKAGEDFEGIPIYGTVAEAKKQTGATVSVIYVPPPFAAAAIDEAVEAGLDLVICITEG
                     IPVRDMIRTRDRMRGTKTLLLGPNCPGTITPDEIKIGIMPGHIHRKGRIGVVSRSGTL
                     TYEAVGQLTALGLGQSSAVGLGGDPVNGLKHIDLLKLFNDDPGTDAVIMIGEIGGSDE
                     ENAARWAKANMKKPVVGFIAGVTAPAGKRMGHAGAIISGGKGTAQEKLAVMEECGIKV
                     TRNPAEMGKLLKSVI"
     gene            11449..>11991
                     /locus_tag="VFV55_03290"
     CDS             11449..>11991
                     /locus_tag="VFV55_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4943350.1"
                     /translation="MSEAVPATPFLKRLAPEARDLLLAAARPVSFVKGATLVRHGQAA
                     RGAFVLREGQAEARVPAPGGECFVVARLGSGDIFGEMALVEAGTCTATVVATANVDGW
                     FLAFEDFRAVISQRHPAARELQHAVTLVLAERLRAVNLRLAAIASPEDRPSRPIPEQF
                     LAGMGRARRASFDLAAFLPRL"
BASE COUNT         1995 a         4080 c         4030 g         1886 t
ORIGIN      
        1 taggccacgg aggcggttgc cacgtacttg accgcggggt ggccgccgat gacgagctgc
       61 ggcggatcct tcgggaaaag cttcttgccc tcggggatcg ccgggccggg cggcgtgaag
      121 gtcgtgttcg cgccatacgg cgtcgtgggc gaggtctgga tgccggtgtt ggtgtacgcc
      181 tcgttgtcgt agcagatgaa gagcgcgtca tggccgcggt acatcatggc ggacatggcc
      241 tgcagcccga tgtccacggt gccgccatcg ccggccatga cgatgatgtt ggggaattcg
      301 cccgtgcgct tgcccttgcg cagcagcacc ttgtaggcgg cctcgacgcc cgaggcgacg
      361 gcgccgccgt tggtgatctg cgtgtgcatc cacgggacca tgtacggcgt gcagaggtag
      421 ctcgcgttcg ccacgtacat gcagcccgtg ggcccgagga agatcgtgtt gggaccggcc
      481 gccttggcga ccagcttgta ctgcagcgcc gggccgcatc ccgcgcaggt ccggtggccg
      541 gggacgtagt attcctcctt cggcacgttc cggatcagct tgaccggcgg aagcgtttgg
      601 tgtttcagga cggcagccat cgtggcctcc ttgtcatcaa gcggttgcgg tttcttcctg
      661 tttcactttg gcgtcctcgg cttcgaagcc gacccagtac acggtctgcc gggtcctgcc
      721 ctccttgagc gccttcgcga gcactccggc catgtagtag aagtcgttga gcggcaccat
      781 ctcgccgccg agaccggcca tgaacgaggt cgtgagcggg cgcttctcca gctcgtagag
      841 cgaggcacgg acttcgtgca ggaggttccc gccgcgcatg gcgccgccgt aggaggtgct
      901 cgtctcgacc acgcccacgg ccttgaactt cgcgagtgcc tgcgcgactt cctgcgtggg
      961 ccacgggcgc acccagcgca gcttcaccag ccccgccttc acgccctggg cccgcaggtg
     1021 cttcacggcg tggcgcgcgg tcatcgtgtg cgcgcccatg atgaagaagc cgatgtcggc
     1081 atcctcggtc atgaactcct cgacgaacgg cgagtagttg cgcccgaaga tgcggttgta
     1141 gtcgtcggtc gcttccttga tcacggccgg cgtgtcgagc atcgcctgcg cgcgctgcat
     1201 gtccatcacc gtgccctggt cggggcggat ctgcgggccg tgcgagacgg ggcgcttggg
     1261 atcgagcggg tgcggcaggt tgtagggcgg caggaagtca tcgacctgcg cctggtccgg
     1321 gatcttcacg aggcccggga tgtgcgagac gaagtagccg tcctggcaga cgtattgcgg
     1381 caggagcacc cggtggtcct cgccgacgcg gtagttgatg aggcagttgt cgaaggcctc
     1441 ctgcggcgtc tcggcccagc ccatcagcca gccctggtcg cggcacgaca tcgcgtcggt
     1501 gtgctcgctg ccgaagtcgc cgggcgggtc gagcgcgcgg tcggcgatcg ccatctgcag
     1561 gggcaggcgt ccgccggcga tgggcgagta gttctcgaac gcgtaggtga ccccgacccc
     1621 cgagctgccg gtgagcgctc gcgcgcctgc ggcagacgca cccatgacca ccgcgagctg
     1681 cgcgtgctcg ccttcgccat ggacgaactc ggcgtcgagc tcgccgttgg ccaccatctt
     1741 cgcgagctcc atcatgatcg cggtgtaggg ccggatcgga tacgagcaga tcacctcgac
     1801 ctgggccatc ttggcggcct gcgcgaagga cgcgcagccg ctgagcatca tctcttttgc
     1861 catggtcttg cctcctcaga acggcttgtc gagccggacg acgccatcgt cgaaatccag
     1921 ctcgtaggcg gcggtcagcg ctcccggggc gcactcgttg atgcagatgt tgcagccctt
     1981 gcagtacttc tcgttgagca ccacctgctc gccggcgtcg tcgagcgaga agcacgcgtc
     2041 ggggcagaac acgatgcagt tcatgcagag gtcgcacttc tcggcctcga gcaccggccg
     2101 gtactcgcgc cagttgcccg tgatcatcat cggttgctcg ccgaccggag cgggcgtcac
     2161 gcacccgagc gggacctgct tgtcactggg gatgttgaac attggcgtcc tccgattcag
     2221 tgcagggaca tcacgaccgc ctggtcgtgg cccttgtgca gcgcgtcctt gttcttcacg
     2281 accgcgtcga ggttctcgag cttcaccagg cccgtcgtgc cccgcgcgag cgcgcccagg
     2341 atcggcgccg cgatgccctg gccgatgccg agcgaatcca cgccgccctc gctcccggag
     2401 aagtcgatcg tctggtcgcc gcagatcgtc ttcgcgtcca cgcagacgat ctggtcgagg
     2461 aggttccggt tgggaatgaa cttgaggatg tcctcgggct tgcggtcggt ggcgaccacc
     2521 agcgtcccgc ctttctccat gccgcgaagg atgtcgattc ccttcacgat ggtcgcgtcg
     2581 gcgacgatca ccacctcggg atgcgcgttc tcgtactcgt agcgctcctc gatctcctcg
     2641 tcgctcacgc gggcgtactt gcgcagcggg acgttgatcc ggtcgggaag gtcgacgtag
     2701 ttgtcgaagg cctgcgtgaa cttgccttcc ttgtccgccg cgcgggcaag gccgttggcg
     2761 atgtcccgcc cctccttgtc ctgaaccacc ccccgagccc ataccgtgat ctggtgcacc
     2821 ttgctggcca tgagagcctc ctttgttgcc ctgggtgttg cgtccggtcc gacctctgaa
     2881 ccacttctag aacgcgcgtc cggtatcggt ttgatctgag tcaacgaagt cgccaattcg
     2941 cttatggttt attgtggtta caatagcttt cagcttatga ggcgagggga tggagggtca
     3001 tgaagaacgc gacctttcgc cagctcgagg tgttcgaggc ggtggcgcgg cacggcagct
     3061 actcgcgcgc cgccgcggag ctgcgcatga gccagccggc ggtgtcgatc cacgtgcggc
     3121 agctcgaaac ccatgcgggg gtgccgctgt tcgagcagtt cggcaagagg atccacctca
     3181 ccccggccgg cgaggaagtc ctgcgctaca gccgcgccat catccagcag cgccgggaag
     3241 tcgacggggc gctcgcggcg ctgaagggaa tccgcggcgg ccggctcaac atcgccgtga
     3301 tcagcgccgg cgactatttc tttcccgggc tcctcgccga attctgccgc cgccacgaag
     3361 gcgtcaccgt gcggctcgcg gtcaacaatc gcgaggaaat cctgcgccag ctcaccgagg
     3421 acacgacgga tctcacggtg ctgctgcgcc cgcccgaaga ctccgacatg gtcgccgagg
     3481 cgttcgcgcc gcagccgcac gtcatcatcg cggcgcccga ccaccagctg gtgcgccggc
     3541 ggcagattcc cctgcgggaa ctggcgcagg aagccttcat cgtgcgcgaa cagggttcgg
     3601 acacgcgcct ggcgatggac gaactgctcg cggagtccgg cgtcaggttc aacatcacga
     3661 tggaaatcag gagcaccgag accatcaagc aggcggtcat cgccggcatg gggatcagct
     3721 ttctctccgc ccataccctg gggcgggagc tcgaggacgg gcaactcgcg gtgctggacg
     3781 tcgagggatt cccggtcatg cgcgagtggc acatcgtgca ccaccggaac aagcgcctgc
     3841 cgccggtggc cgtggcgttc aaggacttcc tcctgcagga aggggcggcg cggatcgaac
     3901 aattgctcgg gcgcgccgcg gcgaagcgca agcccaccgc gtgaggggaa ggtcgagggc
     3961 agcctcgggg ccctcggtgt cgaaggcatt ttcgggcgac aatagcgacc ccgctccgcc
     4021 aacacgagac cccccatgcc ccacgcgatc cgattccaca agaccggcgg ccccgaagtc
     4081 ctccagtggg aggacgtgcc cgtcgcagac cccgcccccg gcgaggcgcg catccgcctg
     4141 aaggcgatcg gcctcaacta catcgacact taccatcgca gcggcctgta tccgctgccc
     4201 ctgccgtccc cgatcggccg cgagggcgcg ggcgtggtgg aggcggtcgg cgccggcgtc
     4261 accgacctga agccgggcga ccgcgtggcg tggacgaccg gcccgctggg aacctatgcc
     4321 gaagtgaaga accatcccgc cgagcggctc gtgaagctcc ccgacgcgat ttccttcgag
     4381 caggcggcgt gcatgatgct gcagggcctc acggtccact acctgctgcg caggacctat
     4441 cccgtgaagg cgggcgaaac gatcctcttc cacgccgccg caggcggcgt ggggctcatc
     4501 gcatgccagt gggcgaaggc gctcggcgcc acgctgatcg gcaccgtcgg ctcggaggaa
     4561 aaggcgaagc tcgcgctcgc gaacggctgc gcgcacgcga tcatctacac gaaggaggac
     4621 ttcctcgccc gcgtgaagga gatcaccggc ggcaagggcg tgccggtcgt gtacgactcc
     4681 atcggcaagg acaccttcat gaagtcgctc gactgcctgc agccgcgcgg cctcatggtc
     4741 accttcggca atgcgtcggg gcccgtggag ccgttcaatc tcggcatcct cgcccagaag
     4801 ggttccctct acgtgacgag gccgacgctc gacacgcacg tgggcaagcg cgaggaactg
     4861 ctggccgcca cgaaggagct cttcgaccac gtgctcgccg ggcgcatcag gatcgcgccg
     4921 cggcagtcgt acgcgctcaa ggatgcggcg caggcacacc gcgacctcga ggcgcgcaag
     4981 accacgggat ccacgatcct ggtgccgtga tcccgggcgg gctcgtcgcc gtcctgcact
     5041 gagcaccgcc atggccctgc ttctcaagga aagggatgtc gagcagctgc tcacgatgcc
     5101 gctcgcgatc gacctggtcg agcgcgtcca ccgggaatac tcggctggca acgcgatcga
     5161 cattccacgc gagcgcacga ggctgccgaa ggcggcgctg cacatcctgc agggggccgt
     5221 gccgtcggcc ggcgtgttcg gctacaaggc ctatacctcc agccgggagg gcatccgctt
     5281 catcgtctac gcgttcaacg ccgaacgcgg aaacctcgac gccatcgtgg aggcgaatta
     5341 cctcggcatg atccgcaccg gcgcggcggg aggggtcgcc gcgaaatggc tggcgcgtcc
     5401 ggatgcgaag gtggcgggcg tgttcggctc cggctggcag gcgcagggcc agatcgaagc
     5461 gctcgccgcg gtgcgggcgc tcgaccgcgt gaaggtctac tcacgcaagg cggagaagct
     5521 cgcggctttc tgcaagcgga tgggcgacag gctgggcctc gacgtggtgc ccgcgggcac
     5581 gcccgaggaa accgtgcgcg gcagcgacgt cgtggtgacc atcaccactt ccgcgacgcc
     5641 ggtcttcgac ggcaactgga tcgagcccgg ccagcacatc agcgccgccg gctccaattc
     5701 gctgcttcgc caggagatcg acgagacggc cgtgcgccgc tgctcgccca tcgtcgtgga
     5761 ctcgcgtccc acggcagtca aggaagccgg ggacctcctt ccggcactgg aaaagggacg
     5821 gctccacgcc gggcagctgg cggaaatcgg cgaggtgatc gacggcaccc gggccggccg
     5881 gaccatgccc gagcaggtga cgcttttcga gtcgcagggg atggccatcc aggacctcgc
     5941 catcgtggcc gagctcgcga agatggcgcg ggcccggggg ctcggcacgg agatcgacgt
     6001 cgggacttga gggaccgtgt aagatttctt ctcctggcgc tgcatcctgt cgaagcgcac
     6061 cgtccgggcg ccgggcaaga acaagggaga atccgtgaag agccaattct ggaaggccga
     6121 ctggtttttc ggcctggtga tcgccatcgg cgtgctcgtc ttcaactcgg cctcgaacct
     6181 gatcccgggg ctggagcgct gggcctacga cctcggcgtg aagatgacct ccaagaaccc
     6241 gagcgacagg atcgccgtgg tcgcgatcga cgagcagtcg atcgccaaca tcggccgctg
     6301 gccgtggccg cgcgaggtgc aggcgaagct catcgaccag ctttcggccg cccgggcgag
     6361 ggtgatcggc aatacggtct tctttttcga gccgcaggtg gaccccggcc ttgcctacgt
     6421 cgagaagatg ctcgcgatct acgacagggc ctaccccggt gcggcgccgc aggaggcgcc
     6481 cggcgtcgcg gccatcgggg cggtcacgcc cgccgcgcag gtggcccagc ccgcgggcga
     6541 ggtggccgag atcggccggt tgctgcgcga ggcctccgtc gcgctcaaca acgacatccg
     6601 ccttgccgcc agcgtgaaga aggcgggcaa tgtagtggtg ccgatggtct tcgacgagat
     6661 gacgaacgcg ccgccgccgg gcaggcccga caagccgctc ccggaatacg tggcgcgcaa
     6721 cgccatcggt agtgcctcgc aggcgggggg ctcgctcatc cacggcgtct cgctcatcgc
     6781 gcccatcgag cagatcggcg ccaacgtggt ggcgatgggc cacctcaatg ccacgcccga
     6841 cccggccgac ggcgccatcc gcagcgagcc gctggtgatc gactactacg gccagcagtt
     6901 cccctcgctc gcgctcatga tcgcggcgaa gagcctgaac ctgggggcca aggacatcaa
     6961 ggtgaacccg ggcgagagcg tggcgctcgg caacaaggtc gtgcgcaccg acgagcaggc
     7021 caagatgttc acctacttct acaaggaccg cgacggacgc gccgccttcc ccgtcgactc
     7081 gttcttcgac gtctacaccg gcaagattcc ggcggccaag tatgccgaca agatcgtgct
     7141 catcggcgcg gtggccgcgg gcatcggcac caaccaggtg acgccgatat cggcgcagat
     7201 gaacccggtc accacgctcg cgcactccgt gtcgtcgctg ctgcaggagc acttcttcgt
     7261 cgcccccacc tggggcatct acgcgacgat cggcgcctac ctgctggtgg cggcctatct
     7321 cgtcgtgctg ctgccgcgcc tgggggccgg catgggcgcc atcctcacgg cgctgctctt
     7381 cgtggcgctg ttcggcgccc acctcgggct catgaccggc gcgggcctgt ggctgcagct
     7441 catgcttccc gccaccctgc tcgtgatcgg gcacctgctc ctcaccacca agcgcttcct
     7501 cgtgaccgag cgcggcaagg aaagatcgga catcgaaggc gcggaatcca accgcatgct
     7561 cggcctcgcc ttccagcagc agggccagct cgacatggcc ttcgacaagt tccgcaagtg
     7621 cccctatgac gagcagctgg gcgagaacct ctatgcgctc gccctcgact tcgagcgccg
     7681 ccgccagttc aacaaggcgg aatccgtgtt cggccacatc caccggcagg acccgaaatt
     7741 caaggacgtg gccgagcgca tgcagcgcgc caagaccatg agcgagacga tcatcctggg
     7801 cggctcctcg gccgcccgct cgagcgcggc gacgatggtg ctgcagggcg ggcagacgga
     7861 gaagccgatg ctcggccgct accaggtgga gaaggagctg ggcaagggcg ccatgggggt
     7921 ggtgtacctg ggcaaggacc cgaagatcgg ccgcacggtg gcgatcaaga cgatggccct
     7981 tgcctccgag ttcgaaggcg acgagctgca ggaggcgaag gaacgcttct tccgcgaggc
     8041 ggagacggcc gggcgcctca cccaccccaa cgtcgtcacc atctacgacg ccggcgagga
     8101 gcacgacctc gcctacatcg ccatggagtt cctcaggggg aaggacctcg tgccctacac
     8161 caagccggac acgctgctcg cgcccgacaa ggtgctgtcc atcgtcgagc gcgtcgccga
     8221 cgcgctgggc tacgcgcatt cgatgggcgt ggtgcaccgc gacatcaagc cggccaacat
     8281 catgtacgag ccggagagcg acaccgccaa ggtgaccgac ttcggcatcg cgcgcatcac
     8341 cgactcgtcc aggaccaaga ccggcatggt gctgggcacg ccgtcctaca tgtcgcccga
     8401 gcagctggcc ggcaagaaga tcgacggccg ctcggacctc ttttccctcg gcgtcaccct
     8461 cttccagttg ctgagcggtc ggctaccctt cgagggcgag tcgatgacgc agctcatgtt
     8521 cgccatcgcg agcaatccgc acccgcccat ccgcgagatc aacccgcagc tcgcgccgtg
     8581 gatcgacgcg atcatcgaca aggcgctcgc caaggacgtg aacgtgcgct accagacggg
     8641 cgccgagttc gcggaagcca tccgccaggc gcgcaagggc ggcccctcgg tcaatgtctc
     8701 tctctgagcc aaaggcgaag cgcgagcccc tgcaccgccg cacgatcgag atcgtcggct
     8761 acaagcgcga ggacggcctc ttcgacatcg aggggcgcct gctcgaccgc aaggacgtgg
     8821 agtttcccgt cggcggccgc cgcaagctgc ccggcgagcc ggtgcacgag atgtggctgc
     8881 gcatcaccgt ggaccgcgag ctgcgcatcg tggacgccga ggcggccacc gacgccatgc
     8941 cctacgtggg gcactgcgac cgcatcacgc cggattacgc gaagctcatc ggcctttcca
     9001 tcgggccggg ctacctgcgc aaggtgaagg agctcctcgg cggcatccac ggctgcacgc
     9061 acatcacgga actcgcgggg tcgctcgcca cggccgcgtt ccagaccttc gccgggcagg
     9121 ggctggcccc gcccgaccgc aagccccccc agctcgaccg gtgccacgcg ctcgacgttc
     9181 acgcggaggc cgtgcggctt ttctatcccc gctggtatcg cggcacgacg ccggtcggcg
     9241 aacccgggcc gaccgagaac cactgaacgc atcttcgccg ccggatgatc ggcgtcaagg
     9301 ctcgggcggg cgggccggct gctagaatgc ccgggctccc acaatcctgc tccccccacg
     9361 gccatgaaca tccacgaata ccagggcaag gaaatcttcc gcaagttcgg cgtgcccacg
     9421 ccgcgcggca tccccgcgtt ttccgtcgac gaggcggtga aggcggccga gactctcggc
     9481 ggaaaggtct gggtggtgaa ggcgcagatc cacgccggtg gccgcggcaa gggcggcggc
     9541 gtgaaggtcg ccaagtcgcc ggcggaggtg aaggatctcg ccggaaagat cctcggcatg
     9601 acgctggtca cgcaccagac cgggcccgag ggacgcaagg tgcgccggct cctcatcgag
     9661 gagggcgcgg acatccgcaa ggaactgtac gtggccctcg tggttgatcg cgtcacgcag
     9721 cgtgccacga tgatggcctc gagcgaaggc ggcatggaca tcgaggaagt cgcggcgcat
     9781 acgccggaga agatccaccg cgtggcgatc gaccccagga ccggcctcaa ggatgccgaa
     9841 gccgaggaca tcgcgcgcaa gatcggcgtt cccgagggct ccatcgccca ggcgcgcgac
     9901 ctgctgaaga acctgtaccg ggtcttcgac gagtgcgact gctcgctcgc ggaggtgaac
     9961 ccgctcatcg tcaccggcga gggcaaggtg gtcgccctcg acgccaagct caatttcgat
    10021 tccaacgcgc tcttccgcca ccccgagatc gtcgcctacc gcgacctcga cgaggaggac
    10081 cccgcggagg tcgaggcgtc gaagttcgat ctttcctaca tctcgctcga cggcaacatc
    10141 ggctgcctgg tgaacggcgc ggggctggcc atggcgacca tggacacgat caagctcttc
    10201 ggcggcaacc ccgccaactt cctcgacgtg ggcggcggcg ccacgacgga gaaggtgacg
    10261 gaagccttca agatcatgct cgcgcgcccc ggcctcaagg cgatcctggt gaacatcttc
    10321 ggcggcatca tgaagtgcga cacgatcgcc aacggcgtgg tcgcggcggc gaaggaggtg
    10381 cacctgtcgg tgccgctggt cgtgcgcctg gagggcacga acgtggacat cggcaagaag
    10441 atcctcgccg actccgggct gccgatcatc tcggcatccg acatgggcga cgccgcggcg
    10501 aaggtcgtgg ccgccgcaaa gggctaaggg aacggacatg agcatcctga tcaacaagga
    10561 caccaaggtc atcacgcagg gcatcaccgg caagaccggc cagttccaca cgaagatgtg
    10621 ccgcgactac gccaacggga agaactgctt cgtggccggc gtgaacccga agaaggcggg
    10681 cgaggatttc gagggcatcc ccatctacgg cacggtggcc gaggcgaaga agcagacggg
    10741 cgccacggtc tccgtgatct acgttccgcc gcccttcgcg gccgcggcga tcgacgaggc
    10801 ggtggaagcc ggcctcgacc tcgtgatctg catcaccgag ggcatcccgg tgcgcgacat
    10861 gatccgcacg cgcgacagga tgcgcggcac gaagacgctg ctgctgggcc cgaactgccc
    10921 cgggacgatc acgcccgacg agatcaagat cggcatcatg cccggccaca tccacaggaa
    10981 gggccgcatc ggcgtggtgt cgcgttccgg cacgctcacc tacgaagccg tggggcagct
    11041 caccgcgctg ggcctgggac agtcatccgc cgtcggcctc ggtggcgatc cggtcaacgg
    11101 attgaagcac atcgacctcc tcaagctgtt caacgacgac cccgggaccg acgccgtcat
    11161 catgatcggc gagatcggcg gctccgacga ggaaaacgcc gcgcgctggg cgaaggccaa
    11221 catgaagaag cccgtcgtgg gattcatcgc gggcgtcacc gcgcccgcgg gcaagcgcat
    11281 ggggcatgcc ggcgcgatca tctcgggcgg caagggcacg gcgcaggaga agctcgcggt
    11341 gatggaggag tgcggcatca aggtgacgcg caatcccgcg gagatgggca agctcctcaa
    11401 gtcggtgatc tgagcgccgg gccgggcatc gcgcccggcc cttcccccgt gtccgaagcc
    11461 gtcccggcca cgccgttcct gaagcgcctt gcgcccgagg cgcgcgacct gctgctcgcc
    11521 gccgcgcgtc ccgtatcctt cgtgaaaggc gcgaccctcg ttcgccacgg ccaggccgcc
    11581 cgcggcgcct tcgtcctgcg cgaaggccag gccgaggccc gggtgcccgc gcccggcggg
    11641 gaatgcttcg tggttgcccg gctcggcagc ggcgacatct tcggcgagat ggccctcgtg
    11701 gaggccggca cctgcaccgc tacggtcgtc gccaccgcca atgtggacgg ctggttcctc
    11761 gccttcgagg attttcgcgc cgtgatctcg cagcgccatc ccgctgcccg cgaattgcag
    11821 cacgcggtga cgctggtgct cgctgaaagg cttcgcgcgg tgaacctgcg gctggccgcg
    11881 atcgcctcgc ccgaggatcg tccttcgcgc ccgatacccg agcaattcct cgccggcatg
    11941 ggacgcgccc gccgcgcctc gttcgatctt gcggcattcc tgccgcgcct c
//
LOCUS       DASUEV010000101         5702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_602256, whole genome shotgun sequence.
ACCESSION   DASUEV010000101 DASUEV010000000
VERSION     DASUEV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602256"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VFV55_03295"
     CDS             <1..1381
                     /locus_tag="VFV55_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4943351.1"
                     /translation="PDDRARVRDTRERLLSGEIGHIATEYRVRRAQDEWITVGVNGYA
                     IKDPTRSKERQLRIIGFVSDVSERIRAEAERQALAAQLRQAEKMEAVGRLAGGIAHDF
                     NNILGAILGYGELAQSRAQADPQLKRYVDTIVGAGNRAKALVAQILAYSRAESGVKEP
                     VHLGAVIEEVCELLRGSSAANVEVRLVAPQEETLVMGDPTRLHQLMMNLSSNAIQAMP
                     DGGVLEIALTRRTLAAPVRTRLAEVAPGEWAVLEVKDTGQGIPPAVIDRIFEPFFTTK
                     PAGRGTGLGLALVHSIVGEHGGAIDVASEEGRGTTFTIWLPRLEEGAGGAAAAEAPAS
                     AGRGQVILAVDDEPEVLAALEEMLATLGYEPAGYTDSRAALEAFRADPRRFEAVISDE
                     VMPGLTGTQLVLELRRVNPAIPIVIATGFGGAGFETRALAVGVNRVLRKPYRMQEIGE
                     ALRLFFGAS"
     gene            1495..2385
                     /locus_tag="VFV55_03300"
     CDS             1495..2385
                     /locus_tag="VFV55_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4943352.1"
                     /translation="MLETGQSSAGVPGMAGTAPAWRVGTSFQPLFSVRDAALAGFEAL
                     ARPMSGAIASVRPSAYFAAHGGADLARIDRECRHAHLSRFASLDQGEGFLCLNMHPRS
                     ALADVTAADLRAELGLHGLEPSRICIELLGDASGDEKLLAEVVAACRAIGVRVTMDNF
                     GVAHSNFDRVMGLAPDFVKIDRSLVGEAAGCAKARCLLPSVIRLLHDAGTQVIVEGIE
                     EAPHALLAIEAGADFVQGYYFGAPRAVLSAEPLTLRLLAELKKVHRAPVPDPDDCDVD
                     PANFCLARLLMAGRALAVEG"
     gene            complement(2401..3540)
                     /locus_tag="VFV55_03305"
     CDS             complement(2401..3540)
                     /locus_tag="VFV55_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX4943353.1"
                     /translation="MEREQSLKFMHDLLRALIARKGSDLFITAGFPPAMKVDGKVMPV
                     SQQSLSPVHTQELARSIMTDKQAAEFEATNECNFAVSPTGIGRFRVNAFVQQARVGLV
                     LRTITTTIPKFEELGLPAVLKDVAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENSYG
                     HIITIEDPVEYVHEHRNCIITQREVGVDTDNWFNALKNTLRQAPDVILIGEIRERETM
                     EYGIAFAETGHLCMSTLHANSTNQALDRIINFFPEERRAQLLMDLSLNLKAIISQRLI
                     PKKGTKGRVAAIEIMLNSPLMQDLIFKGEVHEMKALMARSRELGMQTFDQALFDLFEQ
                     DQISYEDALRNADSVNDLRLKIKLESKNAKNRDLGSGLEHLEIVK"
     gene            complement(3557..4600)
                     /locus_tag="VFV55_03310"
     CDS             complement(3557..4600)
                     /locus_tag="VFV55_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX4943354.1"
                     /translation="MDIAELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVPPLEH
                     KDVHGMVYDIMNDSQRKVYEETLEVDFSFEIPNLARFRVNAFNQQRGAGAVFRTSPSK
                     VLSLEDLHAPRIFAEIAKYPRGLVLVTGPTGSGKSTTLAAMVNDVNENEYGHILTVED
                     PIEFVHEAKKCLINQREVGPHTLSFANALRSALREDPDIILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTVDRIIDVFPAAEKEMIRAMLSESLRAVISQTLCKTKDGSG
                     RVAAHEIMIGTPAIRNLIREAKVAQMYSAIQTGQSIGMQTLDQNLQDLVKRNLVSIPE
                     ARMKASNKDAFVG"
     gene            4689..5390
                     /locus_tag="VFV55_03315"
     CDS             4689..5390
                     /locus_tag="VFV55_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX4943355.1"
                     /translation="MSPIAENLQGMRRRISEALQGDGRAVTLVAVSKTHPPGLVREAF
                     AAGQRDFGENYVQEAVAKIGALADLPATWHFIGALQGNKAKEVARHFDWVHGVDRARI
                     ADLLSRHRPAGKPPLNVCVEVNISEEATKGGVKPGEALALARHVASLPGLRFRGLMGM
                     ASPSGDEAKARAEFTVLRRELEAIRAAGLAGDTLSMGMTHDYRAALAEGATMLRLGTA
                     IFGAREAQPWREQQA"
     gene            5387..>5702
                     /locus_tag="VFV55_03320"
     CDS             5387..>5702
                     /locus_tag="VFV55_03320"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX4943356.1"
                     /translation="MRITFLGGGNMGAAIIGGLVERGFSAAGITVIDPGQAAREGLVS
                     RFGVAVFAAAGAGLPAADALVLAVKPQQMREAVRPLLPLEPSTVVVTIAAGIRIADLS
                     RWL"
BASE COUNT          825 a         1886 c         2038 g          953 t
ORIGIN      
        1 ccccgacgac cgcgcgcgcg ttcgcgacac gcgcgagcgg ctcctctccg gggagatcgg
       61 ccacatcgcc accgagtacc gcgtgcggcg tgcgcaggac gagtggatca cggtcggcgt
      121 gaacggctat gcgatcaagg atcccacgcg cagcaaggaa cggcagctgc gcatcatcgg
      181 gttcgtgagc gacgtgtcgg agcgcatccg cgccgaggcc gagcgccagg cgctggccgc
      241 gcagctcagg caggcggaga agatggaggc ggtcgggcgc ctggcgggcg gcatcgccca
      301 cgacttcaac aacatcctgg gcgcgatcct ggggtacggg gagctcgcgc agtcgcgcgc
      361 gcaggccgac ccgcaactga agcgctatgt cgacacgatc gtgggcgcgg gcaaccgcgc
      421 caaggcgctc gtcgcgcaga tcctcgccta cagccgggcc gagagcgggg tcaaggagcc
      481 ggtgcacctc ggcgcggtga tcgaggaagt ctgcgagctg ctgcgcggct cttcggcggc
      541 caacgtcgag gtgcgcctcg tggcgccgca ggaggagacg ctggtgatgg gcgatcccac
      601 gcgcctgcac cagctcatga tgaacctctc ctcgaatgcg atccaggcaa tgccggacgg
      661 cggcgtgctc gagatcgccc tcacgcggcg cacgcttgcc gcgcccgtgc gcacgcgtct
      721 cgcggaggtc gcacccggtg aatgggccgt gctcgaggtg aaggataccg ggcagggcat
      781 tcccccggcg gtgatcgacc gcatcttcga gcccttcttc accaccaagc ccgcggggcg
      841 gggaaccggc ctcggcctgg cactcgtgca ctcgatcgtg ggcgagcacg gcggggccat
      901 cgacgtggcg agcgaggagg ggcgtggcac cacgttcacc atctggctgc cgaggctcga
      961 ggaaggggcc ggcggcgccg cggccgcgga ggcgccggcc agcgcgggcc gcggccaggt
     1021 catcctcgcc gtggacgacg agccggaggt gctcgcggcc ctcgaggaga tgctcgccac
     1081 cctgggctac gagccggccg gctacacgga cagccgggct gccctggaag ccttccgcgc
     1141 cgacccgcgc cgcttcgagg cggtgatcag cgacgaggtg atgccgggcc tcaccggcac
     1201 gcagctcgtc ctggagttgc ggcgcgtgaa tcccgccatc ccgatcgtca tcgccacggg
     1261 cttcggcggc gcgggcttcg agacacgcgc gctggccgtc ggcgtgaacc gcgtcctgcg
     1321 caagccctac cggatgcagg agatcggcga ggcgctgcgc cttttcttcg gcgccagctg
     1381 atgcccttgc aattgtaatt gcggccatcc ggggcgtaat cgcagggtag ttcgcgccgc
     1441 cgagaatgac gcggcgcgcg ggaatttccc cgccgcagga atcggggtgc ggtcatgctg
     1501 gaaacggggc agtcgtcggc cggcgtgccg ggaatggcgg ggacggcccc cgcatggcgc
     1561 gtcgggacga gtttccagcc gctgttttcc gttcgcgatg ccgcgctcgc cggattcgag
     1621 gcgctggcgc ggcccatgag cggcgcgatc gcgtccgtgc ggccatccgc ctatttcgcg
     1681 gcccacggcg gggccgacct ggcgcgcatc gaccgcgaat gccggcatgc ccacctgtcg
     1741 cgcttcgcga gcctggacca gggcgagggc ttcctctgcc tcaacatgca cccgcgctcc
     1801 gcgctggccg acgtcacggc tgccgacctg cgcgcggagc tggggctgca cggcctggaa
     1861 ccctcccgga tctgcatcga gctgctcggg gacgcctccg gcgacgaaaa gctgcttgcc
     1921 gaggtggtcg ccgcctgccg cgccatcggc gtgcgcgtga cgatggacaa tttcggcgtc
     1981 gcgcactcca atttcgaccg cgtgatgggt ctggcgccgg atttcgtgaa gatcgaccgc
     2041 tccctggtgg gcgaggcggc gggctgcgcc aaggcccggt gcctcctgcc ctcggtcatc
     2101 cggctcctgc acgatgcggg cacgcaggtc atcgtggagg gcatcgagga ggcgccgcac
     2161 gcgctgctcg cgatcgaggc cggcgcggac ttcgtgcagg gctactattt cggcgcgccg
     2221 cgcgcggtgc tctcggccga accgctcacg ctgcgcttgc tggccgagct gaaaaaggtg
     2281 catcgggcgc ccgttccgga cccggacgac tgcgatgtcg atcccgccaa cttctgcctg
     2341 gcgcgcctgc tcatggccgg gcgcgccctc gccgtcgagg gctgaaggcg ccgcccgccg
     2401 tcacttgacg atctcgaggt gctccagccc cgagccgagg tcgcggttct tcgcgttctt
     2461 gctctcgagc ttgatcttga ggcgaaggtc gttgaccgag tcggcgttgc gcagcgcatc
     2521 ctcgtagctg atctggtcct gctcgaagag gtcgaagagc gcctggtcga aggtctgcat
     2581 gcccagctcg cgcgagcggg ccatgagcgc cttcatctcg tgcacctcgc ccttgaagat
     2641 gaggtcctgc atgaggggcg agttcagcat gatctcgatg gccgccacgc ggcccttggt
     2701 gcccttcttc gggatgaggc gctgggagat gatggccttg aggttcagcg acaggtccat
     2761 cagcagctgg gcgcggcgct cctcggggaa gaagttgatg atgcggtcga gcgcctggtt
     2821 ggtggagttc gcatgcaggg tcgacatgca caggtggccc gtctcggcga aggcgatgcc
     2881 gtattccatc gtctcgcgct cgcggatctc gccgatgagg atcacgtcgg gagcctggcg
     2941 cagcgtgttc ttgagcgcgt tgaaccagtt gtcggtgtcc acgcccacct cgcgctgcgt
     3001 gatgatgcag ttgcggtgct cgtggacgta ctcgaccggg tcctcgatgg tgatgatgtg
     3061 gccgtagctg ttctcgttgc ggtgcccgat catcgcggca agcgaggtcg acttgcccga
     3121 gcccgtgccg ccgacgaaga tcaccaggcc gcgcttggtc atggccacgt ccttcagcac
     3181 cgcgggcagt cccagctcct cgaacttggg gatcgtcgtg gtgatcgtgc gcagcaccag
     3241 ccccacgcgg gcctgctgga cgaaggcgtt cacgcggaag cgcccgatgc ccgtcgggct
     3301 gacggcgaaa ttgcactcgt tggtcgcctc gaactcggcc gcctgcttgt ccgtcatgat
     3361 cgagcgggcg agctcctgcg tgtgcacggg cgacaggctc tgctgggaaa ccggcatcac
     3421 cttgccgtcc accttcatcg ccggcgggaa tcccgcggtg atgaagaggt ccgagccctt
     3481 gcgcgcgatg agggcgcgca gcaggtcgtg catgaacttg agagattgtt cgcgttccat
     3541 ggtgccctcc ggcctttcag ccgacgaatg cgtccttgtt ggaggccttc atgcgcgcct
     3601 ccgggatcga gacgaggttc ctcttcacca ggtcctggag gttctggtcg agcgtctgca
     3661 tgccgatcga ctggccggtc tggatcgcgg agtacatctg cgccaccttc gcctcgcgga
     3721 tgaggttgcg gatggcgggg gtgccgatca tgatctcgtg cgccgccacg cggccgctgc
     3781 cgtccttggt cttgcacagc gtctgcgaga tcaccgcgcg cagcgattcg gaaagcatcg
     3841 cgcggatcat ctccttttcc gccgcgggaa acacgtcgat gatgcggtcc acggtcttcg
     3901 cggcggagga cgtgtgcagc gtgccgaaca cgaggtggcc cgtctcggcc gcggtcatcg
     3961 ccagccggat cgtctccagg tcgcgcattt cgcccacgag gatgatgtcc gggtcctcgc
     4021 gcagcgcgct gcgcagggcg ttggcgaacg acagcgtgtg cgggccgacc tcgcgctggt
     4081 tgatgaggca cttcttggcc tcgtgcacga actcgatcgg gtcctccacc gtgaggatgt
     4141 ggccgtactc gttctcgttc acgtcgttga ccatcgccgc gagcgtggtg gacttgcccg
     4201 agcccgtggg cccggtcacc agcaccaggc cgcgcgggta cttggcgatc tcggcgaaga
     4261 tcctcggggc gtgcaggtcc tcgagcgaga gcaccttcga gggactggtc cggaacaccg
     4321 cgccggcgcc gcgctgctgg ttgaacgcgt tcacgcggaa gcgcgcgagg ttcgggatct
     4381 cgaaggagaa gtcgacctcc agcgtttcct cgtacacctt tcgctgggag tcgttcatga
     4441 tgtcgtagac catcccgtgc acgtccttgt gctcgagcgg cggcacgttg atgcggcgga
     4501 cgtcgccgtg cacgcggatc atcggcggca gcccagcgga caggtgcagg tccgaggcct
     4561 tgttcttcac ggagaatgcg aggagttcgg cgatatccat ttcttcgtcg cggctgtgga
     4621 aggcaatggc gcgtgaaggg accggcgttc agccagagta aaatgcgcca ctcgtaccct
     4681 gaccgattat gtctccaatc gcggaaaacc tgcaaggaat gagacggcgc atttccgagg
     4741 cgttacaagg agatggccgc gccgtcacgc tcgtggcggt gtccaagacg cacccgcccg
     4801 ggctggtgcg cgaggccttt gccgccgggc agcgcgattt cggcgagaac tacgtgcagg
     4861 aggccgtggc caagatcggc gcgctcgcgg accttcccgc gacgtggcat ttcatcggcg
     4921 ccctgcaggg aaacaaggca aaggaggtcg cgcggcattt cgactgggtg cacggcgtgg
     4981 accgcgcgag gatcgccgat ctcctctcgc gccatcgtcc ggcgggcaag ccgcccctga
     5041 acgtctgcgt cgaggtcaac atcagcgagg aggcgaccaa gggcggcgtg aagcccggcg
     5101 aggcgctggc cctcgctagg cacgtcgcgt cgctgccggg actgcgcttc cgcggcctca
     5161 tgggcatggc gagcccttcg ggcgacgagg cgaaggcgcg ggccgagttc acggtgctgc
     5221 gccgcgagct ggaggcgatc cgcgccgcgg gcctcgcggg cgacacgctg tcgatgggca
     5281 tgacgcatga ctaccgggcc gcgcttgccg aaggggccac gatgctgcgc ctcggcacgg
     5341 ccatattcgg cgcgcgcgag gcgcaaccct ggcgggagca gcaggcatga ggatcacctt
     5401 tctcggcggg ggcaacatgg gcgcggccat catcggtgga ctggtcgagc ggggattttc
     5461 cgcggccggc atcaccgtca tcgacccggg ccaggccgcc cgcgagggcc tcgtctcgcg
     5521 cttcggcgtg gccgtgttcg cggccgcggg cgcggggctt cctgccgccg atgcgctcgt
     5581 cctggcggtg aagccgcagc agatgcgcga agcggtgcgg ccgctgctcc cgctcgaacc
     5641 ttccaccgtc gtcgtcacca tcgcggccgg catccgcatc gcggacctgt cgcgctggct
     5701 gg
//
LOCUS       DASUEV010000102        14092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_716170, whole genome shotgun sequence.
ACCESSION   DASUEV010000102 DASUEV010000000
VERSION     DASUEV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 14092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14092
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716170"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..2490)
                     /locus_tag="VFV55_03325"
     CDS             complement(235..2490)
                     /locus_tag="VFV55_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4943357.1"
                     /translation="MNHFAAYLPAFLLAVILPVAALAEEPAPGNVSPATAVGTDEFPP
                     AVAARPLPASPPVAPAREVRETLHGVEVIDPYRWLEDVKLPESQAYFQAQDEYARKAL
                     ARIPGRAALAERIRALSAAEVTVRSLALANGKVFYLKLAPGQETPVLYVREGFAGAER
                     VLVDPTRFSTEAARAAIDWFRPSPDGRLVAYGVSLGGTEDATLRVLEVRNARDTGVAI
                     DRARFNAGLSWHHDSRAFYYTRIPEGGAEDSAHRYAGIRLYRHVIGRPAGQDEVIFAS
                     GAGGARDVPEFAYPALVVPEDSTHAFAIVRHGARREIAVHMTTMVDLAKGLPRWSKIV
                     DFDDGVTAIQAWKDDLYLLTHKGAPRNKVLLVRAGRDGISRPRPAVPEGDAVILDMAL
                     AADALYLRSTVGGLERLERLNLGFGASRTPEFVKTAFDVSITQLLTNPRRPGAVLYLQ
                     GFLEPPAVVTVEAKTGNLHNTGLQPAPRADVSEMDEVRLYATSHDRVKVPITLLYRKK
                     TMLTADNPTLLIGHGAYGMSQAPVFDATRLAWLERGGIIAFAHVRGGGEYGEEWHRDG
                     QKAKKGNTILDFIACAEFLVKYGFTSPRRLAVQGSGAGGIPAAGAMVRRPDLFAAVVP
                     RVAMLDMLRFELAPGGPANIPEFGSVATREGFEALLGMSAYQQVKDGTAYPAVLLTAG
                     MNDPRVDPWQAAKMTARLQAASAGGKPVLMRVEWQGGHGTGATRTQKDEELADIYAFL
                     LWQFGHPDFMP"
     gene            complement(2607..5627)
                     /locus_tag="VFV55_03330"
     CDS             complement(2607..5627)
                     /locus_tag="VFV55_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943358.1"
                     /translation="MDNSTVFTKTAKGITQVNQRSASLSKDLMRILRLVDGKSNFEQI
                     LAKAELDKAVLEKAMNTLIKDGFARIFETRKDQDPFGEDDFDFTAPGKLPAQAQRVVP
                     AAANDVGELVRQRAKGEADKKARESAQAAAREKARQQAEARATAEAEARAKVEAEMRA
                     MEQARRAKEASERARAELDAKMREEEARRRAVAEHQAKLAAERKAKEEEESRRLAEMK
                     AKAQAEANARARAELETRVREEEARKRLVSEQVARAMAEEKLKEEAESQRLAEVRAKA
                     EAEARALAEARSRAEAEAQALAKARAEAEASVRRHAEQAAASEQELKVRLKEEIETRI
                     RGEMEVLLRDEIGEKARAEMQAQIMQEARLAAKAEFEERLQEEREALQRAGTEVRAQA
                     ESAAKARAEQEAKLRAEAEARAVAEGAAREKAEEETRRLRRLEARARGEVEAAAREKA
                     ETASLAARERAESAARLEAERKAKVEAEARATVEAEERERREKELAATIDQERRAREE
                     AEKRARIEARARESVEETTREKVKAELEGDMTRRAELEGKAQAKAYMQAKQQAELDED
                     ARIRSDQEKKAREIAEVLRTKVEPDVKDEAPAARRGPRKKVNWARNIVAGLAAILVIG
                     VGLLHVVPLRPFADKLEKGIGGWLHEEVSISSLTFSLIPSPHLKIAGFTVGKALDAKA
                     ASGRVFLDIPALLGDRVVVNTLVLENVSITGDAPRRIFSWGKVEGKSAAAEIDAVQLK
                     NVRLDVKPDLKPFDATLSFGRDGTFTNASLSGEGKWTATIRPGESGYDVTISARNWEP
                     PIGAPVAVSDLTAKGVLTASDITFPEFEAVLLDGKMTGSLKVSWGPSINLASSFSLAR
                     VRAEQLVGAFTRSIAVTGKVGGNFTVGAESATLEKLFAAPRVQGKFQLTDGSISNADL
                     VASMQSSDAAGRAGVTKIADLTGDYGVVDGRASFRNLNLHGGVLRGSGGIEIGANGAL
                     SGRLQVEIRSNIAQDRGSFAVSGTVAKPILRRGG"
     gene            5902..7902
                     /locus_tag="VFV55_03335"
     CDS             5902..7902
                     /locus_tag="VFV55_03335"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX4943359.1"
                     /translation="MNRFAAVLLSLAVLPLAAWADPPGRVARLALVEGQASVFTDPEQ
                     GWEDARINTHLTSENSLWTEPGARAEVRFGSTAIRLGEVTQLDILQLDDSSFHGHVAR
                     GTLTVRIRDFDRAESYAVTTPDARFQLRSNGRYRIDADLERGESRLTVFSGTARLDTV
                     GGSISIDTSRSVRVTGGERPRYEFEAAYATAIDEWALARDQRFDERETSRYVPPQMTG
                     WEDLDDYGVWRNEPEYGPVWFPTRVEAGWAPYRYGRWTWVRPWGWAWVDDAPWGYAPF
                     HYGRWAFIGSRWGWYPGHYTTRPVWAPALVGWVGRPGWNISISTGLTDVVGWYPLSPY
                     DRFQPWYATNVTYVTNVNRIVIPPRWNRDRRDDRRPDRRDDRGDNRHDNRDRGATVVP
                     RNQFGSHRPVQNVLAPVSRDVVAAQPVVSGSSVLPSRNEWRSRPKPVQSSPAPRAAAS
                     GEPVGSPRMSSPLAPAPGAPAARPRATTPAAPAYAAPQAAPQVAPRVAPRVAPQAAPQ
                     AAPQVAPRVAPQAAPQAAPQSAPTSRFARPAPAEPALRAKPAPVEPATQAKPPEPRAI
                     ELRVPRAIERAAPVQVQPHVQPEAQPRMQPQAQPQAQPQLQSSPLQQRVQPQAQPQPA
                     AVPVAPAANPAPVQERPARGGERPSPRPVEKPARPADTGQGG"
     gene            7945..9153
                     /locus_tag="VFV55_03340"
     CDS             7945..9153
                     /locus_tag="VFV55_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4943360.1"
                     /translation="MQSARPFWTLAIAGAAILAVTMGIRQSLGLFVSPLNTATGLGIV
                     TVSFTLAVGQFVWGAAQPVFGAIADRVGPVRVMMAGGLLLAIGLAATPFMGSSLGLVF
                     FLGVVTAAGAGAGSFSILMGAAANRMPPERRAFASGFINAGGSFGQFVFAPLSQALIA
                     TVGWIAALWALAAAALATIPMSVAFRERHGEAHHGAHAAGLTLTGQIAQAFRDPSYWC
                     LHAGFFTCGFHIAFLVTHLPGEVTLCGLPASVSAVSLAIIGLANIAGSLGAGWLAQTQ
                     RMKWILFWMYGTRAIAVAVYLLLPKTTATFYAFAAVLGLTWLATVPPTAGLVGKLFGT
                     RFLATLFGLTLLSHQIGGFFGAWLGGLAIQRTGNYNWMWYADMALAAAAAVVNLPIRE
                     AIPQPVAKAA"
     gene            complement(9199..10236)
                     /locus_tag="VFV55_03345"
     CDS             complement(9199..10236)
                     /locus_tag="VFV55_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4943361.1"
                     /translation="MKTNPLGRDGPAVTVATLGTMTFGRQNTEAEAHDQLDLALERGV
                     NLVDAAEMYPVPAAERTHGFTERYVGSWLAKGRRDRVMVATKITGPGRKFAWMRDGRQ
                     ALTEEQIVLACEGSLARLGTDYLDLYQLHWPSRNVPKFGGWSFDPAAEVQATPVDEQL
                     GALDRLVRAGKVRRIGLSNETPWGVMSFLAAVRGSGLARVASVQNAYNLLNRVAESGL
                     SEVLWREQVGMLAYSPLAFGYLTGKYRDGVPARSRVGELENFGARYSKPGVAPAVAAY
                     CALAASRGLSPVQLALGFVASRPFVTSTIVGATSRAQLVENLDAIATPLDRETLDAID
                     RIHLLHTNPAP"
     gene            complement(10246..10455)
                     /locus_tag="VFV55_03350"
     CDS             complement(10246..10455)
                     /locus_tag="VFV55_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX4943362.1"
                     /translation="MEMKRLNATGLRSAGYDEKNRKLVVETTAGTYEYANVSPEVYRR
                     LMTSPSPASYYRESIEEEFTGRKLR"
     gene            complement(10481..11074)
                     /locus_tag="VFV55_03355"
     CDS             complement(10481..11074)
                     /locus_tag="VFV55_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX4943363.1"
                     /translation="MRIAAALVVLLATPALAASEEFVVESAHTYPSFEVSHLGISTQR
                     GRFEHTSGRILLDREAGTGTIEVVIDTPTISTGNRVLDAMLKGEDFFNVERFPAMTFR
                     ARSMEFEGGEPKRATGELTLLGVTRPVEMSFVGFACTRLPFLVRLTCGADVVAKIKRS
                     DFGMTAYSAFVGDEVRLEIQVEAVKQEADAQPPPAGE"
     gene            complement(11078..11563)
                     /locus_tag="VFV55_03360"
     CDS             complement(11078..11563)
                     /locus_tag="VFV55_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4943364.1"
                     /translation="MNRDVRPPDLADVFSRLASMPGFLDAALARADRSLLARRAADGG
                     FSLVEHACHLRDLEREGYLLRVMRLLAEDRPVLQGFAGDRIAEERDYQSQDAAAAARD
                     FAAARAEVLRVARSLGAGELARAGEFAGRPVSLAGVLAMAGDHDAEHRSQIDRLLAEL
                     G"
     gene            complement(11560..12162)
                     /gene="msrA"
                     /locus_tag="VFV55_03365"
     CDS             complement(11560..12162)
                     /gene="msrA"
                     /locus_tag="VFV55_03365"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX4943365.1"
                     /translation="MRILKILSLAVALAAPAVAIAQGATEKATFAGGCFWCMEPPYDS
                     IPGVISTTSGYTGGRTANPTYAQVSSGTTGHAEAIQIVYDPKKVTYEKLLEVFWVNID
                     PTVRDRQFCDGGTQYRTGIFYHSEAQRKAAEASKAALEKSKPFKAAIVTPVEMAGTFY
                     PAEDYHQDYYLKNPVRYKFYRNGCGRDARLRELWGDKAGK"
     gene            complement(12215..12838)
                     /locus_tag="VFV55_03370"
     CDS             complement(12215..12838)
                     /locus_tag="VFV55_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX4943366.1"
                     /translation="MSATIASARLHLALLAAWSVLFLLTGWSPKDRLTWVLEVAPAVA
                     AVVIIAAIYARWRFTPLVLVLILVHCAVLMIGAKYTYAEVPWFDWLRAELGLARNHYD
                     RLGHFVQGFVPAMIAREILLRKAVLPRGRWLFFLVTCVCLAISATYEFVEWGVALASG
                     EAATAFLGTQGDVWDTQWDMFMALWGAIVAQVSLAGTHDRQLGLGNP"
     gene            complement(12835..13737)
                     /locus_tag="VFV55_03375"
     CDS             complement(12835..13737)
                     /locus_tag="VFV55_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX4943367.1"
                     /translation="MERIGIDLGGSKIEIAVLDANGAEALRRRVATPAGDYAAILEAI
                     AGLVANAERERGVRCTVGMAMPGALSLATGLVKNSNSVCLNGRPLKADLESRLQREVR
                     LANDANCFALSEAVDGAGRGATIVFGAILGTGVGGGIVVDRQVLAGANAIAGEWGHNP
                     LPQSGAGDGPLPRCYCGREGCVETFLSGPGIAADHERATGDRIEAAEIARRAGEGGAT
                     CEATLCRYEERLARSLAVVMNIVDPDVIVLGGGLSNLARLYARVPDLWGAHVFSDQVR
                     TRLVPPVHGDSGGVRGAAWLWGRP"
BASE COUNT         2028 a         5079 c         4698 g         2287 t
ORIGIN      
        1 gcccctccag gcgcgggcga agccatcggg ccaccttgac cgcctgcctg cggcccttgt
       61 cggtgagctc gcgccgcatg tcgaccacgc ccgcttccgc atccgcgtgg cgccacagga
      121 ccagttccat tgttcgctct ccttggtgtc agacacgatg ttcgttgtgt gtcagtgtca
      181 gactccagac ccgccaactt cgagaggagt ctgacaccag caagcatcga agattcacgg
      241 catgaagtcc gggtggccga attgccagag gagaaaggcg tagatgtcgg ccagttcctc
      301 gtccttctgc gtgcgcgtcg cgcccgttcc atgcccaccc tgccattcga cgcgcatcaa
      361 cacgggcttg ccgccggcgg aggcggcctg caggcgcgcg gtcatcttgg ccgcctgcca
      421 ggggtccacg cgcgggtcgt tcatgccggc ggtgaggagg accgccggat acgcggtgcc
      481 gtccttcacc tgctgatagg cggacatccc gagcaacgcc tcgaacccct cgcgcgtggc
      541 gacggagccg aactccggga tgttggccgg gccgccgggc gcgagttcga agcgcagcat
      601 gtcgagcatc gccacgcgcg gcacgaccgc ggcgaagaga tcgggccggc gcaccatcgc
      661 accggccgcg gggatgccgc ctgcgcccga gccctggacc gccaggcggc gcgggctcgt
      721 gaagccgtac ttcaccagga attccgcgca ggcgatgaaa tcgagaatcg tgttgccctt
      781 cttcgccttc tgcccgtcgc ggtgccactc ctcgccgtac tcgccgccgc cgcggacgtg
      841 cgcgaaggcg atgatgccgc ctcgctcgag ccacgcgagc cgggtggcgt cgaagaccgg
      901 cgcctgcgac atgccgtagg cgccgtggcc gatgagcagc gtcgggttgt cggccgtgag
      961 catcgtcttc ttgcgataga gcagcgtgat cggcaccttg acgcggtcgt ggctcgtcgc
     1021 gtacaggcgc acctcgtcca tctccgaaac gtcggcccgc ggcgcgggct gcaatcccgt
     1081 gttgtgcagg ttcccggtct tggcttccac ggtcaccacg gcgggcggct ccaggaagcc
     1141 ctgcaggtac aggaccgcgc cagggcgccg cggattcgtg aggagctggg tgatggacac
     1201 gtcgaacgcg gtcttcacga attccggcgt gcgcgatgcg ccgaagccca ggttcaggcg
     1261 ctcgagccgc tcgagcccgc cgaccgtgct cctgaggtac agggcgtcgg cggccagtgc
     1321 catgtcgaga atgaccgcat cgccttccgg caccgcggga cggggccgcg agatcccgtc
     1381 cctgcccgcc ctcaccagaa gcaccttgtt gcgcggtgcc cccttgtggg tgagcaggta
     1441 gaggtcgtcc ttccacgcct ggatggcggt cacgccatcg tcgaaatcga cgatcttcga
     1501 ccagcgcggc agccccttcg cgaggtcgac catcgtcgtc atgtgcacgg cgatctcgcg
     1561 acgcgcgccg tggcgcacga tcgcgaacgc atgcgtcgaa tcctcgggca cgacgagcgc
     1621 cgggtaggcg aactccggaa catcgcgcgc gccacccgcg ccggaggcga agatgacctc
     1681 gtcctggccg gccgggcgcc cgatcacgtg ccgatacagg cgaatgcctg cgtagcggtg
     1741 cgcggagtcc tcggccccgc cctcgggaat gcgcgtgtag tagaacgcgc ggctgtcgtg
     1801 gtgccaggaa aggcccgcgt tgaagcgcgc gcggtcgatg gccacgccgg tatcgcgcgc
     1861 gttgcgcacc tccagcacgc gcaatgtcgc gtcctccgtg cccccgaggg ataccccgta
     1921 ggccaccagc cggccgtcag gcgacgggcg gaaccagtcg attgcggcac gcgccgcctc
     1981 ggtcgagaat cgggtggggt cgaccagcac ccgttccgcg cccgcgaacc cttcgcgcac
     2041 gtacagcacc ggcgtttcct gccccggggc gagcttcagg tagaacacct ttccgttggc
     2101 aagcgcgagg ctgcgcaccg tgacctccgc agccgacagc gcgcggatgc gctccgccag
     2161 ggcggcgcgc cccgggattc gcgcgagcgc cttgcgcgcg tactcgtcct gcgcctggaa
     2221 gtacgcctgg gactcgggca acttcacgtc ttccagccac cggtagggat cgatcacttc
     2281 gacgccgtgc agcgtctcgc gaacttcgcg cgccggcgcc accgggggcg atgccgggag
     2341 cgggcgcgcc gccacggcgg gcgggaattc gtccgtgccc actgccgtcg ccggtgacac
     2401 atttcccggc gccggctctt cggcgagagc cgccacggga aggatcacgg cgagcaggaa
     2461 ggcgggcagg taagcggcga agtggttcat cgtggtgact cgtcggtcgt catggcgaac
     2521 tgctgcgcag ccagccgcgc acgaagtccg gcgggcccgc gagcgcttcc cgccaccccg
     2581 cctggcgagg cagtgccaat tcggtgtcag ccgccgcggc gcaggatcgg cttggccacc
     2641 gtacccgaca ccgcgaacga accgcggtcc tgggcaatgt tggaacggat ctccacctgg
     2701 agcctgcccg aaagggcgcc gttggcgccg atttcgatgc cgccgctgcc gcgcaggacg
     2761 ccgccatgca gattgaggtt gcggaacgat gcgcgcccgt cgacgacgcc gtaatcgccg
     2821 gtcaggtcgg cgatcttggt tacgcccgcg cggcccgccg cgtccgacga ctgcatggac
     2881 gcaacgaggt ccgcgttcga aatggagccg tcggtgagct ggaacttgcc ttgcacccgg
     2941 ggcgcggcga aaagcttctc gagcgtcgcc gattcggccc ccaccgtgaa gttccccccc
     3001 acctttccgg tcacggcgat actgcgcgtg aaggcgccca caagctgttc cgcgcgcacg
     3061 cgggccagcg agaagctgga ggcgagattg atgctcgggc cccagctgac cttgagtgag
     3121 ccggtcatct ttccgtcgag caggacggcc tcgaactccg ggaacgtgat gtcgctagcc
     3181 gtgagcaccc cctttgccgt gagatcgctc accgccaccg gcgcgccgat gggcggttcc
     3241 cagttgcgcg cgctgatggt gacgtcgtat ccggattcgc ccgggcggat ggtcgccgtc
     3301 cacttgccct cgcccgacag gctcgcgttg gtgaatgtgc cgtcgcggcc gaaggaaagg
     3361 gtcgcgtcga acggcttcag gtcgggcttc acgtccagcc tcacgttctt gagctgcacg
     3421 gcgtcgattt cggccgccgc gctctttcct tcgacctttc cccacgaaaa aatgcgccgg
     3481 ggggcgtcgc ccgtgatcga gacgttctcc agcacgaggg tgttgacgac cacgcggtcg
     3541 cccaggagcg cgggaatgtc caggaaaacg cggccgcttg ccgccttcgc gtcgagcgcc
     3601 ttgccgacgg tgaaccccgc gatcttcagg tgcggcgacg ggatgaggga gaacgtgagg
     3661 ctcgatatcg aaacctcctc gtgcagccag ccgccgatgc ccttctcgag cttgtcggcg
     3721 aaggggcgca ggggcaccac atgcagcagg cccacgccga tcaccaggat cgccgcgagg
     3781 cccgccacga tgttgcgggc ccaattgacc ttcttgcgcg ggccgcgccg ggcggccggg
     3841 gcctcgtcct tgacgtcggg ctcgaccttg gtgcgcaaca cctccgcgat ctcgcgcgcc
     3901 tttttttcct ggtccgaccg gatgcgcgca tcctcgtcca gttccgcctg ctgcttggcc
     3961 tgcatgtagg ccttggcctg cgccttgccc tccagctcgg cgcggcgcgt catgtcaccc
     4021 tcgagctcgg ccttcacctt ctcgcgcgtg gtttcctcga ccgactcgcg cgccctcgcc
     4081 tcgatgcggg cacgcttttc ggcttcctcg cgtgccctgc gttcctggtc gatcgtggcg
     4141 gccagctcct tctcgcgccg ctcgcgctcc tcggcctcga ccgtcgcgcg ggcctcggcc
     4201 tcgaccttcg ccttgcgctc ggcttcgaga cgcgccgccg actccgcgcg ttcgcgcgcg
     4261 gcgagcgatg cggtttcggc cttctcccgc gcggcggctt ccacttcacc acgggcgcgc
     4321 gcctccaggc ggcgcagccg tcgcgtctcc tcctcggctt tttcccgggc ggcgccttcc
     4381 gcgacggccc tcgcctccgc ctcggcacgc agcttcgctt cctgctccgc gcgtgccttc
     4441 gccgcgctct cggcctgggc gcgcacttcc gtcccggccc tctgcaacgc ctcgcgctcc
     4501 tcctgcaacc gctcctcgaa ctcggccttc gccgcgaggc gcgcctcctg catgatctgc
     4561 gcctgcatct ccgcgcgggc cttctcgccg atctcgtcgc gcaggagcac ctccatctcg
     4621 cccctgatgc gcgtttcgat ctcctccttg aggcggacct tgagctcctg ctccgaggcc
     4681 gccgcctgct cggcgtggcg ccgcacggac gcttcggctt ccgcgcgcgc cttggcgagc
     4741 gcctgggcct cggcttccgc gcgcgacctg gcctcggcga gcgcacgcgc ttcggcctcg
     4801 gccttggcgc gcacctccgc gaggcgctgc gattccgcct cttccttcaa cttctcttcc
     4861 gccatcgccc gcgccacctg ttccgagacc aggcgcttgc gcgcttcctc ctcgcggacc
     4921 cgggtctcga gttccgcgcg agcccgtgcg ttggcctccg cctgggcctt cgccttcatt
     4981 tcggcgaggc gccgggactc ttcctcctcc ttggccttgc gctcggcggc aagcttcgcc
     5041 tggtgctcgg ctacggccct gcggcgagct tcctcttcgc gcatcttcgc atcgagctcg
     5101 gccctggccc gttcggaggc ttccttggcg cggcgagcct gttccatcgc gcgcatctcc
     5161 gcctcgacct tggcccgagc ctccgcctcc gccgtggcgc gcgcctcggc ctgctgccgg
     5221 gccttctcgc gcgcggcagc ctgggcgctc tcgcgcgcct tcttgtcggc ctcccccttg
     5281 gcccgctggc gcaccagctc gccgacgtcg ttggccgcgg cgggcacgac acgctgcgct
     5341 tgcgcaggga gcttgcctgg cgcagtgaag tcgaaatcgt cctcgccgaa cgggtcctga
     5401 tccttgcgcg tctcgaagat gcgcgcgaag ccgtccttga tcagggtgtt catcgccttc
     5461 tcgaggaccg ccttgtcgag ctcggccttc gccaggatct gctcgaagtt ggacttgccg
     5521 tcgacgagcc tgaggatgcg catcaggtcc ttcgacaggg aggcggaacg ctggttgacc
     5581 tgcgtgatgc ccttggcggt cttggtgaag acggtgctgt tgtccatccg atggtcccgg
     5641 ggcgaattct tctttgctgg cggaaggcgg ctggcgggcg agaaaaccgg cccggatacg
     5701 caggcccccg atttcccgtg ccaacgaaat tatccaagcc aataatggca tgaattcagg
     5761 atgttggaaa atcgcttcgt ctgcgacccg ggcacgacag atcttgttac agtaaatgct
     5821 gtcctccggt tgacggtccg gcgcgttatg gattcatgga accggcgctt cgccggcgat
     5881 caaccggaag gcaggaccgc gatgaaccga ttcgccgctg tgctcttgtc ccttgccgta
     5941 cttccgctcg ccgcgtgggc cgatccgccc ggccgcgtgg cgcggcttgc cctcgtcgag
     6001 ggccaggcct ccgtgttcac cgaccccgag caggggtggg aggatgcgcg catcaacacc
     6061 cacctcacca gcgagaactc gctgtggacg gagccgggcg cccgcgccga ggtccgcttc
     6121 gggtccacgg ccattcgact tggcgaggtt acgcagctcg acatcctcca gctcgacgac
     6181 tcttcgtttc atggccatgt cgcgcgcggc acgctcacgg tgcgcatccg cgatttcgac
     6241 agggccgagt cgtacgccgt caccacgccc gatgcccgct tccagttgcg cagcaacggg
     6301 cgctaccgga tcgacgccga cctcgagcgc ggcgaatccc ggctcaccgt cttttccggc
     6361 accgcgcgcc tcgatacggt cggcggctca atcagcatcg ataccagccg cagcgtgcgc
     6421 gtgaccggcg gcgagcgccc gagatacgaa ttcgaggcgg cctacgccac tgccatcgac
     6481 gaatgggccc ttgcccgcga ccagcgcttc gatgagcgcg agacttcgcg ctacgtgccg
     6541 ccgcagatga cgggctggga agacctggac gactacggcg tctggcgcaa tgaaccggaa
     6601 tacggtcccg tctggttccc gacccgcgtg gaagcgggct gggcccccta ccggtacggt
     6661 cgctggacgt gggtccgccc ctggggctgg gcctgggtcg acgacgcgcc gtggggttac
     6721 gcgcctttcc actacgggcg ctgggcgttc atcggcagcc ggtggggctg gtatcccggc
     6781 cactacacga cgcgcccggt gtgggcgccc gccctggtcg ggtgggtcgg ccggccggga
     6841 tggaacatct ccatatcgac gggacttacc gacgtggtcg gctggtatcc gctctcgccc
     6901 tacgaccgct tccagccctg gtacgcgacc aacgtcacct acgtgaccaa cgtgaaccgc
     6961 atcgtgatcc cgccgcgctg gaatcgcgac cgccgcgacg atcgccgccc cgaccggcgc
     7021 gatgaccgcg gcgacaaccg ccacgacaac cgcgaccgcg gtgccaccgt cgtgccacgc
     7081 aaccagttcg ggtcccaccg gccggtgcag aacgtcctgg cgcccgtttc ccgcgacgtt
     7141 gtggctgcgc aacccgtcgt atccggctcg tcggtactgc cgtcgcgcaa cgaatggcgc
     7201 tcgcgtccga aacccgttca atcgtcccct gcgcctcgtg cagccgcgag cggcgaacct
     7261 gtcggaagcc cgcggatgtc gtctcccctt gcacccgctc ccggagcgcc tgcggccagg
     7321 cccagggcga ccactcccgc ggcgcctgcc tacgctgcac cccaggccgc gccccaggta
     7381 gcgccccgag tagcgcctcg agtagcaccc caggcagcgc cccaggcagc gccccaggta
     7441 gcgccccgag tagcgcccca ggcagcgccc caggcggcgc cccagtccgc accgacatcg
     7501 cgtttcgcgc gacctgcacc cgcggagcct gccctgcgcg ccaagccggc acccgtggaa
     7561 cccgcgacgc aggcgaagcc gccagagcct cgcgccatcg aattgcgcgt gccgcgcgct
     7621 atcgaaaggg ctgccccggt gcaggtgcaa cctcacgtgc aacccgaggc gcaaccgcgg
     7681 atgcaacctc aggcgcaacc gcaagcgcaa ccgcagctgc aatccagtcc gcttcagcag
     7741 cgggtacagc cgcaggcgca gcctcagcct gccgccgtgc ccgtcgcacc tgcggcgaat
     7801 cccgcccccg tccaggaaag accggcccgg ggaggcgaaa ggccctcgcc acggcccgtg
     7861 gaaaagccgg cgaggcccgc ggacaccggc caggggggct gaggactcac gcgctggcgc
     7921 ggggacgatg gtagtctccc gcgcatgcaa tccgcccgac ctttctggac gcttgccatc
     7981 gcgggcgcgg ccatcctcgc cgtgacaatg gggatccgcc agtccctggg gctgttcgtc
     8041 tcgccgctca acaccgccac gggcctcggc atcgtcacgg tgagcttcac gctcgccgtg
     8101 ggccagttcg tgtggggcgc ggcgcagccg gtcttcggcg ccatcgccga ccgtgtgggc
     8161 ccggtgcgcg tcatgatggc gggcgggctc ctgctcgcga tcgggctcgc ggcgacgccg
     8221 ttcatgggat cgagccttgg cctggtcttc ttcctcggcg tcgtcaccgc cgcgggcgcc
     8281 ggcgccggca gcttctccat cctcatgggc gcggccgcca accgcatgcc gccggagcgg
     8341 cgcgccttcg cttccggttt catcaacgcc ggcggctcgt tcgggcagtt cgtgttcgcg
     8401 ccgctctcgc aggcgctcat cgccacggtg ggctggatcg cggctctatg ggcgcttgcc
     8461 gccgcggccc tcgccacgat ccccatgtcg gtcgccttcc gcgagaggca cggcgaggct
     8521 catcacggcg cgcacgccgc gggcctcacg ctaaccgggc agattgcgca ggcgttccgc
     8581 gacccgagct actggtgcct gcacgcgggc ttcttcacct gcggcttcca catcgccttc
     8641 ctcgtgacgc acctgccggg ggaggtgacc ctgtgcgggc ttcccgcatc ggtgtccgcc
     8701 gtgtcgctcg cgatcatcgg tctcgccaac atcgcgggca gcctcggcgc ggggtggctt
     8761 gcgcagaccc aacggatgaa atggatcctc ttctggatgt acggcacgcg cgcgatcgcg
     8821 gtggccgtct acctgctgct gccgaagacg accgcgacgt tctacgcctt cgcggcggtg
     8881 ctgggactca cctggctggc cacggtgccg cccacggccg ggctcgtcgg caagctcttc
     8941 ggcacgcgct tcctcgccac gctcttcggc ctcacgctgc tctcgcacca gatcggcgga
     9001 ttcttcggcg cgtggctggg cggcctcgcc atccaacgca ccgggaacta caactggatg
     9061 tggtacgccg acatggcgct cgccgcggcg gccgcggtgg tcaacctgcc gatccgcgag
     9121 gcgattccgc aaccggtggc aaaggcggct tgagcggcag gagttgaaat ctccctcccc
     9181 tcctgcgacg ccgcaacatc agggcgcggg attggtgtgc agcagatgga tgcggtcgat
     9241 cgcatcgagc gtctcgcggt ccagcggtgt cgcgatggca tccaggttct cgacgagttg
     9301 cgcgcgcgac gttgcgccga cgatcgtgct ggtcacgaaa ggacggctcg ccacgaagcc
     9361 gagggcgagt tgcaccggcg acaagccgcg gctggccgcc agggcgcagt aggccgccac
     9421 ggccggcgcc acgccgggct tcgaatagcg cgcgccgaaa ttttcgagtt cgcccacgcg
     9481 cgagcgcgcg ggaacgccat cgcggtactt gccggtgagg tagccgaagg cgagcgggct
     9541 gtaggcgagc atccccacct gctcgcgcca cagcacttcg gaaagcccgc tctccgccac
     9601 gcgattgagg aggttgtacg cgttctgcac gctcgccacc cgcgcgaggc cgctgccgcg
     9661 caccgcggcg aggaaagaca tcacccccca gggcgtctcg ttggagagac caatgcggcg
     9721 aaccttgccg gcgcgcacca ggcggtcgag ggcgccgagc tgctcgtcga cgggcgtggc
     9781 ctgcacctcg gcggccgggt cgaaactcca gccgccgaac ttggggacat tgcgcgacgg
     9841 ccagtgcagc tggtagaggt cgaggtagtc ggtgcccagg cgcgcaaggc ttccctcgca
     9901 cgcgagcacg atctgctcct cagtaagcgc ctgcctgccg tcgcgcatcc acgcgaactt
     9961 gcgccccggt ccggtgatct tcgtcgcgac catgacgcgg tcgcgccggc ccttcgccag
    10021 ccaggagccc acgtaacgct cggtgaatcc gtgcgtgcgc tccgcggcgg gcaccgggta
    10081 catctccgcc gcgtccacga ggttcacgcc gcgctcgaga gcgaggtcga gctggtcgtg
    10141 cgcctcggcc tcggtgttct ggcggccgaa ggtcatcgtg ccgagtgtcg cgaccgtgac
    10201 ggcagggcca tcgcggccga gtggattcgt cttcacacgc gcgccctagc gcagcttccg
    10261 gccggtgaac tcctcctcga tggattcgcg gtaatagctt gcgggcgaag gggaagtcat
    10321 gaggcgccgg tacacctccg gcgacacatt ggcgtactcg taggtgcccg ccgtggtctc
    10381 gacgacgagc ttgcggttct tctcgtcgta gcccgcggat cgcaagccgg ttgcgttgag
    10441 ccgtttcatc tccatctcgc ttctcccttt gaagtccgca tcactcgccc gccggcggcg
    10501 gttgtgcatc ggcttcctgc ttgaccgcct cgacctggat ctccagcctg acttcgtccc
    10561 cgacgaaggc ggaataggcc gtcatgccga agtccgagcg ctttattttc gccaccacgt
    10621 cggcgccgca ggtgaggcgg acgagaaagg gaagcctcgt gcacgcgaag cccacgaagc
    10681 tcatctcgac ggggcgcgtc acgcccagca gggtgagctc gcccgtggcg cgcttcggtt
    10741 cgccgccttc gaactccatc gagcgcgcgc ggaaagtcat ggcgggaaag cgctcgacgt
    10801 tgaagaagtc ctcgcccttg agcatcgcgt cgagcacgcg gttgccggtg ctgatcgtgg
    10861 gcgtgtcgat gacgacctcg atcgttcccg tgcccgcctc gcggtcgagg aggatgcgcc
    10921 cggaggtatg ttcgaagcgc ccacgctgcg tcgagatgcc gaggtggctc acctcgaacg
    10981 atgggtaggt gtgggcggac tcgaccacga attcctcgga cgcggccagc gcaggcgtgg
    11041 ccagcaggac gacaagtgcg gccgcgatgc gcatcgctca acccagctcc gcgagaaggc
    11101 gatcgatctg gctgcggtgc tccgcgtcgt ggtccccggc catggcgagg acgccagcca
    11161 gcgacaccgg cctgcccgcg aactcgcccg cgcgcgcgag ttccccggca cccagcgagc
    11221 gcgcgacgcg caggacctcc gcgcgggcgg cggcgaaatc gcgcgcggcg gcggccgcgt
    11281 cctgcgactg gtaatcgcgc tcctcggcga tgcggtcgcc cgcgaagccc tggaggacgg
    11341 ggcggtcctc ggccagcagg cgcatgacgc gaagcagata gccttcgcgc tcgaggtcgc
    11401 gaaggtgaca ggcgtgctcg acgagggaga accccccgtc ggccgcgcgt cgcgcgagca
    11461 gcgaccggtc cgcgcgggcg agcgccgcat cgagaaaccc gggcatcgag gccaggcgcg
    11521 aaaacacgtc ggccaggtcc ggtgggcgca cgtcgcgatt catttccccg ccttgtcgcc
    11581 ccacagttcg cgcagccgcg catcgcgccc gcagccgttg cggtagaact tgtagcgcac
    11641 cgggttcttc aggtagtagt cctggtggta gtcctcggcg gggtagaagg tcccggccat
    11701 ttcgacgggc gtgacgatcg cggccttgaa cggcttcgac ttctcgagcg cggccttcga
    11761 cgcttccgct gccttccgct gcgcttccga gtggtagaag atccccgtcc ggtactgcgt
    11821 gccgccatcg cagaactggc ggtcgcgcac cgtgggatcg atgttcaccc agaacacctc
    11881 gaggagcttc tcgtaggtga cctttttcgg gtcatagacg atctggatcg cctccgcgtg
    11941 ccccgttgtg cccgaggaaa cctgcgcata ggtggggttc gccgtgcgcc cgccggtgta
    12001 gccggaggtc gtggaaatca cgcccgggat cgagtcgtag ggcggctcca tgcaccagaa
    12061 gcagccgccg gcgaaggtgg ccttctcggt ggcgccctgc gctatcgcca cggccggggc
    12121 ggccagggct acagcgaggc tcaggatctt caggattcgc atcgacttct ccattcgtgg
    12181 aaaggggcgc aagttgcttc gaccgcgccg gggcctacgg attccccagc cccagctggc
    12241 ggtcgtgtgt tccggccagc gatacctgcg cgacgatggc gccccacagg gccatgaaca
    12301 tgtcccactg cgtgtcccag acgtcgccct gggtgccgag gaaggcagtc gccgcctcgc
    12361 cggaggccag tgccaccccc cactcgacga actcgtaggt cgcgctgatg gccaggcaca
    12421 cgcacgtcac gaggaagaaa agccaccttc cgcgcgggag cacggctttg cgcagcagga
    12481 tctcgcgcgc gatcatcgcc ggcacgaagc cctgcacgaa atggcccagg cggtcgtagt
    12541 ggttgcgcgc cagtcccagc tcggcgcgca gccagtcgaa ccacggcacc tcggcatagg
    12601 tgtatttcgc cccgatcatc agcacggcgc aatgcaccag gatcaggacg aggacgaggg
    12661 gcgtgaagcg ccagcgcgcg taaatggccg caatgatgac gaccgcagcc accgccggcg
    12721 ccacctccag cacccaggtg aggcggtcct tcggggacca tccggtgagg aggaaaagga
    12781 ccgaccacgc cgccagcagc gccaggtgca gtcgcgcgga ggcgatggtg gcactcatgg
    12841 cctgccccac agccacgccg cgccgcgcac gccacccgaa tcgccgtgca ccggcggcac
    12901 gaggcgcgtg cgcacctggt ccgaaaatac gtgcgcgccc cacaggtccg gcacgcgtgc
    12961 gtagagccgc gcgaggttgg acagcccgcc gcccagcacg atcacgtcgg gatcgacgat
    13021 gttcatcacc accgcgagcg atcgggcgag ccgctcctcg tagcggcata gcgtcgcttc
    13081 gcacgtggcc ccgccttcgc cggcacggcg cgcgatttcc gccgcctcga tccgatcacc
    13141 cgtggctcgt tcgtggtccg ccgcaatccc cggccccgac aggaaggttt cgacgcagcc
    13201 ctcgcgcccg cagtagcagc gcggcagcgg tccatccccg gcgcccgact gcggcaaggg
    13261 attgtgtccc cattccccgg cgatcgcgtt ggcgccggcg aggacctgcc ggtccaccac
    13321 gatgcccccg cccacgccgg ttccgaggat cgcgccgaag acgatcgtcg cgccacgccc
    13381 cgcgccgtcc acggcctccg acagcgcgaa acagttggcg tcgttggcga ggcgcacttc
    13441 gcgctgcagg cgcgactcga ggtccgcctt gagcggccgg ccgttgaggc agacggaatt
    13501 ggagttcttc accagcccgg tggcgaggga aagtgcgcca ggcatggcca tgcccacggt
    13561 gcagcgcacg ccgcgttcgc gctccgcatt ggccaccagc cccgcgatgg cctccagaat
    13621 ggccgcgtag tcacccgccg gcgtcgcgac gcgccggcgc aaggcctcgg cgccgttcgc
    13681 gtcgaggacc gcgatctcga tcttgctgcc gccgaggtcg atgccgatgc gttccatggg
    13741 tggcgcgact atagcccaat atttcacgtg aaatgggcag tgtgaaccac gtcacatcgc
    13801 cagcgcggcg cttgtccgat agtggggtca caagcagtga acaaccccgc aagagcgcac
    13861 cttggcgatc gcccgggcca gaaaaccggt ccgggcgcgc catttttttg tgtcttcgcc
    13921 gaattgcagc gacgcgcctc cgtgcctgat acggccggcg aacacgcgaa cgcgaacggc
    13981 gaaggggagt cgggccgggg ctggggcaag tccatcggcg tcctgggcac gcggcgaaag
    14041 gtaggtaggc cggggatggg gcaagttaat cggcgtcctg ggcacgcaag cg
//
LOCUS       DASUEV010000103         5004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1397019, whole genome shotgun sequence.
ACCESSION   DASUEV010000103 DASUEV010000000
VERSION     DASUEV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397019"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1402)
                     /locus_tag="VFV55_03380"
     CDS             complement(104..1402)
                     /locus_tag="VFV55_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4943368.1"
                     /translation="MATDATSALNPGVARREVFAWAMYDFANSGYTTVVLTAVFNAYF
                     VATVAGNAPWATFAWTVALGISYAIVMAAGPVVGAWCDAHAAKKRALAASTVGCVAGT
                     ALLAGAGPGDVAWAVAFIVVSNVAYSLGENLTAAFLPELARPESVGKVSGWGWSLGYF
                     GGLLSLGLCLAWVTSAPARGSTTAQSVPGTMLITAAVFALASLPTFLLLRERAVPVPT
                     SARQSHAFARLARTAREAASYRDLFAVFACGTFYQAGVATVIALAAIYAEQVMGFETK
                     DTILLILAVNVTAAIGAFGFGYAQDRIGKVRALRITLYGWIVMVAVAYFGTTQAVFWI
                     AANIAGLAMGSSQSAGRALVAYFAPPDRSGEFFGLWGVATRLASILGPVTYGAVTWAT
                     GGNHRLAILLTGVFFVVAIACLAPVNERRGHAAALAPQTP"
     gene            complement(1555..2937)
                     /locus_tag="VFV55_03385"
     CDS             complement(1555..2937)
                     /locus_tag="VFV55_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4943369.1"
                     /translation="MTSIPRTTALALAAVLAAGCAAFDPNNLLSRQALPVETPSGTPV
                     PPPASPTLDMSARMAALDFVWSTVGERYYDPMLNGTDWNAARERWRPRALAAGSDDEF
                     WEALDHMTGELHDSHTRVESPARAARNARFESVTLGFTFLPLEGKLAVTSVHPGSDAF
                     WAGVRPGMTLVEVSGEPARSAYARALDETRDGSTAQAKHLSAARRLLTGEPDTTATLA
                     FARGDGTTFTATLKRQRFASPPRVTHRVLPSGLGYIRLTSWEQSMQGPMIEAIAALKG
                     TPGIVIDLRGNPGGSALMVRNVAAQFFKGRIEVGRTLTRSGRPITIAFDWIEVVKVRQ
                     ELEGTGAYLGPVVVLVNASSGSGSELFAGILQSQGRATIVGQTSCGCLLAFMGYAEVP
                     GGGKLAYSEVGFVFPNGKRIEGAGVVPDVPVPLTIADLLVDRDRALEEAQAVLAGKKQ
                     GTRMNTDGHG"
     gene            3028..3774
                     /locus_tag="VFV55_03390"
     CDS             3028..3774
                     /locus_tag="VFV55_03390"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX4943370.1"
                     /translation="MTRPAHASVLAALLAAACASAPAPSPVAPGSPDPAPFSTAAIDV
                     LPAGWKPFRLSRFKKATDYRVVTLENTVALQATADSSASGLSFDTVADVHEYPWLSWK
                     WKVPALNGMANNTLAQVEDSPARIIVVFEGGREKLPAAEQINYDLAKAMGGNELPYAT
                     IMYIWENYLPEGAIISHHFTTRIKMIVAGSGRKDLGTWHEERVNLLEDYRRAFGEEPP
                     RVRAVGIMSDSDNTGARTVAYYGDIHFGHR"
     gene            complement(3783..4496)
                     /locus_tag="VFV55_03395"
     CDS             complement(3783..4496)
                     /locus_tag="VFV55_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943371.1"
                     /translation="MSRLLFFLLLIANLAFGAHLWLTAPPAEPDFSRRERNREEAKIV
                     AVIPPLIAARRAEETRMQMQTLAGAACVEIAGIAAADLPQAREAFAAMQLGDRLVERK
                     VEEITRHWVYIPPAKDRRSAEQAASILRMKGVTDLSVRPDRAISLGVFSTEEAARRYL
                     VLVEAKGATGAQVGPFAKEVKDSVFLVREPDTQLIARLALLQREHATSTLRAVACPAA
                     DAAATSAATPPELPPATSK"
     gene            complement(4505..>5004)
                     /locus_tag="VFV55_03400"
     CDS             complement(4505..>5004)
                     /locus_tag="VFV55_03400"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX4943372.1"
                     /translation="IASQREQLGVTSGYRDAAQLGTDRWAALIAAHHAAAVHQLVVNA
                     GTALTVDALRADGFFMGGLIVPGPALMRRSLDGGTAQLRLTEGVFDPFPKSTPDAITT
                     GSLQAAVGAIERMRAAMIAGGFAPQGLVLSGGAAPALAPQLPMPFTLNDNLVLDGLVL
                     IARAC"
BASE COUNT          820 a         1695 c         1788 g          701 t
ORIGIN      
        1 ggcctgccga gcaccacgcc gagctcggcc cagctgtgcg tgaagaagtt gttggcgagc
       61 gcgagcgtcg gtgcgccgtg ggagatgaag agggatggaa atgtcatggc gtctgcgggg
      121 cgagagcggc agcgtggccg cggcgctcgt tcaccggggc aaggcatgcg atcgccacga
      181 cgaagaatac gccggtaagg aggatcgcga ggcgatgatt gccacccgtg gcccaggtga
      241 cggcgccgta ggtgacgggc ccgagaatgg aggcgagccg ggtggcgact ccccacagcc
      301 cgaagaactc gccgctgcgg tccggcggcg cgaaataggc caccagcgcc cggcccgcgg
      361 actgcgacga gcccatggcg agcccggcaa tgtttgcggc gatccagaag accgcttgcg
      421 tcgtgccgaa ataggccacg gccaccatca cgatccaccc atagagcgtg attcgcagcg
      481 cccgcacctt gccgatacgg tcctgcgcgt agccgaagcc gaaggccccg atggcggccg
      541 tcacgttcac cgcgaggatg aggaggatcg tgtccttcgt ctcgaacccc attacctgct
      601 cggcgtagat cgccgccagc gcgatcacgg tcgccacccc cgcctggtag aaggtgccgc
      661 aggcgaagac ggcgaacagg tcccgatagg aggccgcctc cctggctgtt cgcgccagcc
      721 gggcgaaggc gtgggactgg cgcgcagacg tcggcacggg caccgcgcgc tcgcgcagca
      781 ggaggaaggt ggggagcgac gcgagcgcga aaacggccgc cgtgatgagc atcgtgcccg
      841 gaaccgactg ggcggtggtc gagccgcgcg cgggcgcgct cgtcacccag gccaggcaca
      901 ggcccagcga gaggagaccg ccgaaatagc ccaggctcca accccatccc gacaccttgc
      961 cgacggactc cgggcgcgcc agctccggca ggaacgcggc ggtgaggttc tcgcccaggg
     1021 aataggcgac gttggacacg acgatgaacg cgaccgccca cgcgacatcg ccgggcccgg
     1081 cgcccgccag gagcgccgtg cccgcgacac acccgaccgt gcttgccgcg agcgcccgct
     1141 tcttcgcggc gtgcgcatcg caccacgcgc ccaccaccgg cccggcggcc atcacgatcg
     1201 cgtaggaaat gccgagcgcg accgtccacg cgaacgtggc ccagggcgca ttgccggcca
     1261 ccgtcgccac gaagtacgcg ttgaacaccg ccgtgaggac gaccgtggtg tagccggagt
     1321 tggcgaagtc gtacatcgcc caggcgaaca cttcccggcg cgcgacgccc gggttcagtg
     1381 cggaagtggc gtcagtggcc aaggtcgggg caaatctcgg ggtaaatctc gggaacacgg
     1441 atgaacacgg atggacacgg atgaacacgg ataaaggcgg aagaatgact actcgtgccc
     1501 ctggttgcga tggccggcgg gatgcaatcc gggcacggct cagccatccc gtttctatcc
     1561 gtgtccatcc gtgttcatcc gtgtcccctg cttcttgccc gcaagtacgg cctgcgcctc
     1621 ctcgagcgcg cggtcgcgat ccacgagcag gtcggcgatc gtcagcggaa cgggcacgtc
     1681 gggcaccacg cccgcgccct cgatgcgctt gccgttgggg aagacgaatc ccacctcgct
     1741 gtaggcgagc ttgccgcccc cgggcacctc ggcgtagccc atgaacgcga gcaggcaccc
     1801 gcacgaggtc tggcccacga tcgtcgcgcg tccctgggac tgcaggattc ccgcgaagag
     1861 ctcgctgcca cttccgctcg atgcgttgac cagcacgacc accggaccca gataggcgcc
     1921 ggttccctcg agctcctgcc tgaccttgac cacctcgatc cagtcgaagg cgatcgtgat
     1981 gggcctgccg gagcgcgtga gcgtgcggcc gacttcgatc cttcccttga agaactgcgc
     2041 ggcgacgttg cgcaccatga gtgcggagcc gcccggattg ccgcgcaggt cgatcacgat
     2101 gccgggcgtg cccttcaggg ccgcaatcgc ttcgatcatc ggcccctgca tcgactgctc
     2161 ccagctcgtg agacggatgt agcccagccc cgaaggcagc acgcgatgcg tgacgcgcgg
     2221 ggggctggcg aagcgctggc gcttgagagt cgcggtgaac gtcgtgccgt cgccgcgcgc
     2281 gaaggcgagg gtcgccgtcg tgtccggttc gcccgtgagg agccggcgcg cggcggacag
     2341 gtgcttcgcc tgcgccgtgg agccgtcgcg tgtttcgtcc agcgccctcg cgtaggcgga
     2401 gcgtgcgggc tcgccggaca cctccaccaa tgtcatgccg gggcgcacgc cggcccagaa
     2461 tgcgtccgac ccgggatgga cgctcgtcac ggcgagcttg ccttcgaggg ggaggaacgt
     2521 gaagccgagt gtcacggact cgaagcgggc atttcgtgcc gcgcgcgcgg gcgattccac
     2581 gcgcgtgtgc gaatcgtgca gctcgccggt catgtggtcg agggcttccc agaattcgtc
     2641 gtcgctaccc gcggcaaggg ccctcgggcg ccatcgctcg cgcgcggcgt tccagtccgt
     2701 gccgttcagc atggggtcgt agtagcgctc gcctaccgtt gaccacacga agtcgagggc
     2761 tgccatccgg gccgacatgt ccagcgtggg actggccggc ggtggcaccg gcgtgcccga
     2821 tggggtctcg acgggcaggg cctggcgcga gaggaggttg ttggggtcga acgccgcgca
     2881 ccccgccgcg aggacggcgg caagggcgag ggcggtagtg cgcgggatcg aggtcatccg
     2941 ggccggtcga aagggtggaa gcggcgtgcg gcgcgaatcg cagctagact gtcgatgccg
     3001 ctatccgcgg ccagaataac cgataacgtg acccgtccgg cacacgccag cgttcttgcc
     3061 gccctgctcg cggccgcctg cgcgtccgcg cccgcgccat cgcctgtcgc cccgggttcg
     3121 ccggatccgg cgcccttctc gaccgccgcc atcgacgtcc tgccggccgg atggaaaccc
     3181 ttccggctct cgcgcttcaa gaaggcgacc gactaccggg tagtcaccct ggagaacacg
     3241 gtagccctgc aggcgaccgc ggactcctct gcctccgggc tctcgttcga cacggtggcg
     3301 gacgtgcacg agtatccgtg gctcagctgg aaatggaagg tcccggcgct caacggaatg
     3361 gccaacaaca cgctcgcgca ggtcgaggat tcgccggcgc gcatcatcgt cgtcttcgag
     3421 ggcggaaggg aaaagctgcc ggccgcggag cagatcaact acgacctcgc caaggcgatg
     3481 ggcggcaacg agctgcccta cgcgacgatc atgtacatct gggagaatta cctccccgag
     3541 ggcgcgatca tctcgcacca cttcaccacg cgcatcaaga tgatcgtcgc cggcagcggc
     3601 cgcaaggacc tcggcacgtg gcacgaggag cgcgtgaacc tgctcgagga ctaccgccgc
     3661 gcgttcggcg aggagccgcc acgcgtgcgg gcggtgggca tcatgagcga ctccgacaac
     3721 accggcgcgc gcacggtcgc ctactacggc gacatccatt tcggccaccg gtaaggcggc
     3781 ccctacttcg aggttgccgg tggcaattcc ggcggcgtgg cggccgaggt cgcggccgcg
     3841 tcggcagcgg ggcaggccac ggcgcgcaac gtcgaggtgg cgtgctcgcg ttgcaggagc
     3901 gcgaggcgcg cgatgagctg cgtgtcgggc tcgcgcacga ggaacacgga atccttcact
     3961 tccttggcga acggtcccac ctgcgcgccc gttgcgccct tcgcctccac cagcaccagg
     4021 tagcggcggg cggcttcttc cgtcgagaag acgccgagcg agatggcgcg gtcggggcgc
     4081 acggagaggt cggtcacgcc cttcatgcgc aggatcgagg cggcctgctc ggcggaacgg
     4141 cggtccttgg cgggcggaat gtagacccag tggcgcgtga tctcctcgac cttgcgctcg
     4201 acgaggcgat cgcccagctg catggcggcg aacgcctcgc gggcctgcgg caggtcggcc
     4261 gccgcgatgc ccgcgatctc gacgcacgcc gcgccggcca gcgtctgcat ctgcatgcgc
     4321 gtctcctcgg ccctgcgcgc ggcgatgagc ggcgggatga cggcgacgat cttcgcctcc
     4381 tcgcgattgc gctcgcgcct cgagaaatcc ggctcggccg gcggcgcggt gagccacagg
     4441 tgcgcgccga aggcgaggtt cgcgatgagg agcaggaaga agagcaggcg ggacaagggg
     4501 acgttcagca ggcgcgtgcg atgaggacca ggccgtcgag gaccagattg tcgttcaacg
     4561 tgaagggcat gggaagctgc ggggcgagcg cgggcgcggc gccgccggaa agcacgaggc
     4621 cctgcggggc gaaaccgcct gcgatcatcg ccgcgcgcat gcgttcgatc gcgccgaccg
     4681 cggcctgcaa cgacccggtg gtgattgcat ccggcgtgct cttcgggaag ggatcgaaga
     4741 cgccttccgt gaggcgcagt tgcgcggtgc cgccgtccag cgagcggcgc atgagcgcgg
     4801 gaccgggcac gatgagtcct cccatgaaga atccgtcggc gcgcagcgca tccaccgtca
     4861 gcgcggtgcc cgcgttcacc acgagctggt gcacggccgc ggcatggtgc gcggcgatga
     4921 gcgcggccca gcgatccgtg cccagttgcg cggcgtcgcg atagccgctg gtcacgccga
     4981 gctgctcgcg ctgcgaggcg atga
//
LOCUS       DASUEV010000104         8111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_831436, whole genome shotgun sequence.
ACCESSION   DASUEV010000104 DASUEV010000000
VERSION     DASUEV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831436"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="VFV55_03405"
     CDS             <1..1500
                     /locus_tag="VFV55_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943373.1"
                     /translation="AGNAVEGTGDGIVWSRVGEWRIAKLRVQSPGAVAIRVGLRIGAT
                     RQPWTLRVAGSDDESKALGPVRMAGPLGQAEIHWTPLTEGDAQVIELASPAEEPEPAV
                     EIATLSHLVAGPSARFAKTVRDIGRSGSCNIDYACVPSPSQALRNAANAVVQMLFTVR
                     SGGSFLCTGTLLNDTNPGTQVPYLFSGNHCFDEEFAPFNNATQMQQVANSLNTFFFFD
                     AVACGSLQAPPFVQRFGGATYLYSNLAQDVLLVRLNDWAPAGAFLSGWDPNPLSAGTS
                     VTLLHHPGGDLKKYSTGTTGSLVTLVSPVDAVTGFIPVTYNQGTAEPGSSGAGLLTFS
                     NANGQYLVRGGLWGGSASCTALSSLDYYSRFDVAYPTLRQWLEAANLPEFDVTDLWWN
                     PSEDGWGINLTQHPSGQVFAVWYTYAADSGPLWLVMSAGQWVTSRSFRGKLYRTSGPG
                     YTQLPFNPAAVKQNEVGTLTLDFTSAGSGSFTWVVDGVQGTKVITRQAF"
     gene            1532..2368
                     /locus_tag="VFV55_03410"
     CDS             1532..2368
                     /locus_tag="VFV55_03410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4943374.1"
                     /translation="MAPASLSIAIVTHAPDERLLARTIARAADAMAFARERGSLGSAR
                     LQIVDNGPGDLATMLGQLGEAALARVERAELDVISGQGNVGFGRGHNLAIARSAADFH
                     LVLNPDVEMDRDALDAALRYLDAHPGVGAITPSVRGTDGEREYLVKAWPTPGVLFVRG
                     FVPRFLHGLFRRQLDAYELRHLDWEKEQSPVAIASGCFLLCRGAALAAVKGFDPGFFL
                     YFEDFDLTLRLSRVTTVAYCPQVRIVHHGGKAAAKGWRHIAWFVNSARRFFTLHGRAP
                     AG"
     gene            2510..2674
                     /locus_tag="VFV55_03415"
     CDS             2510..2674
                     /locus_tag="VFV55_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943375.1"
                     /translation="MKELTEHEVLAVAGGLPSGSALDEVSYRAPAEPLRDPLAFAELA
                     TEEHPDRVTD"
     gene            2748..2924
                     /locus_tag="VFV55_03420"
     CDS             2748..2924
                     /locus_tag="VFV55_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943376.1"
                     /translation="MKDIDKKDSPDVSGGVSPDDSGCLPRFPFSIDYPPNPDMPFPEP
                     PRSFDDPVPVVNVK"
     gene            2953..3144
                     /locus_tag="VFV55_03425"
     CDS             2953..3144
                     /locus_tag="VFV55_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943377.1"
                     /translation="MSEPDVPKALEKNELSGTQVEDIAGGGLIDGQTLIDFSASLIQT
                     YDNLVAFASHVIGRVAGEP"
     gene            3213..4082
                     /locus_tag="VFV55_03430"
     CDS             3213..4082
                     /locus_tag="VFV55_03430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX4943378.1"
                     /translation="MPAPTGLQIAFLAFAFEFFSMLASKTLAPLIHWPADQFALLGQL
                     ISFSMATLILFGFPGLRKRCLSELRVPVPAGSSPELIAASIAHLAVPFAVVGGVVMQA
                     FATGSADQLLSRIPTNDPSMAWEDTLSPPGLLRLFLLAWFVGPVIEELVFRGFLYRAF
                     ERQWGWPVSMALTSFLFGLAHPTHIAAAALGSVILICVLRRTGSLRACIFVHITFNIL
                     ISWPLFGQVLLTAPKGLSTSIATWSIQLACLAFAAVALPAYVWMSRKAKLPAGDGEVA
                     ASPVHLGSSRISS"
     gene            4126..5310
                     /locus_tag="VFV55_03435"
     CDS             4126..5310
                     /locus_tag="VFV55_03435"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX4943379.1"
                     /translation="MIRPLSYAALTIVAVAIAVAILSYLFIAEYAKKASLAGTLVPAS
                     GAIRVVAQQPGLVRERRVSEGERVAAGEALLRLVDARATRDDGPVGEATVALAETRIA
                     QTRRQRVEIKAATHSERDALRGRIAGLEEELTHLDGELDALVAREALAQRSLQRFDDL
                     QRRGFVSPAQRQQKEEESLERRSRRHAAARARLGLERELAGLRLAVTESEARSRAQLS
                     ALDAQLAALAQERVERRAQADAVVTAPAAGTVAALLVEPGQVVGSGANLLTLLPDGSP
                     LEAHLFAPSRAIGFIRAGQEVMLRFPAFPYQKFGSHRARVLSVSRSALAPAELGFAPP
                     DGSREPLYRVKVALGTQTVAAYGRAEPLQAGMQVEGDVLLDRRRLIEWVFEPLFSLAG
                     RA"
     gene            5312..7465
                     /locus_tag="VFV55_03440"
     CDS             5312..7465
                     /locus_tag="VFV55_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX4943380.1"
                     /translation="MGPLAGLDFGGARRLPVFLQTEAAECGLASLGMVASFHGHRIDL
                     AALRRRFTLSLKGATLAYLMQVAGRLHLAPRPLRLDLDELPQLKTPCILHWDLNHFVV
                     LKSAAAREVVIHDPAFGVRRLPMAEVSKHFTGIALELVPTAEFRPADERRRVRLRDLT
                     GPVAGLARSLAQVLALALVLQSFAVLAPFYMQWVVDGAVVSADRDLLAVLGLGFLLLA
                     LVQVAVTALRSWVVLHLGTILNIQWLANVFSHLLRLPVAWFEKRHLGDVVSRFGAVTT
                     IQRTLTSSFVEAVIDGLMAVATLAMMLVYSGALTALAVIAVAAYAVSRAAFYAPLRRA
                     TEESIVHAARQQSHFLETVRGVQSIKLFGRQEERRARWLNLVVDAVNRDLVVQKLSLG
                     FRSANGLVFGVERIAVVWVGALLVLDSAFSIGMLFAFMAYKEQFSARVAGLIDKLIEL
                     KMLQLQGERLADIVLTPPEEEARSPAPVVEETSIEVRNLSFRYSDTEPFVLQNCAFRI
                     EPAESVAIVGPSGCGKTTLVKIMLGLLAPTDGQVLVGGIDLQRLGVEPYRRLVGTVMQ
                     DDLLFAGSIADNVSFFDPGPSQEAIERCTKLAAVHDDIATMPMGYHTLIGDMGAALSG
                     GQRQRILLARALYKQPRILFLDEATSALDVQRERQVNAAIRGLNLTRILIAHRPETIA
                     SAGRVIVLQGGKVAQDLRRAVDTSHGSGDAATHAA"
     gene            7535..7948
                     /locus_tag="VFV55_03445"
     CDS             7535..7948
                     /locus_tag="VFV55_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943381.1"
                     /translation="MIHTRKRGRPRTSDLTRAEQLRAAKRAQRRREREAGIAAVELRL
                     PSERATQLRAATAAPRFKALLGSLLDNLVLDLEAWPVLRELAWSRSGRWILAEDALAI
                     YERNWRFVDPRHLGKDEAALIERLKATCGAGVLNA"
BASE COUNT         1197 a         2836 c         2676 g         1402 t
ORIGIN      
        1 gccgggaacg cggtggaagg caccggtgac ggcatcgtct ggagccgcgt gggcgaatgg
       61 cgcatcgcca agctgcgcgt ccagtcgccc ggtgcggtgg cgattcgcgt cggcttgcgc
      121 atcggcgcga cgaggcagcc ctggacgctg cgcgtggcgg gcagcgacga cgaatcgaag
      181 gccctcggcc ccgtccggat ggccggcccc ctggggcagg ccgaaatcca ctggacgccg
      241 ctcacggaag gcgacgcgca ggtgatcgag ctcgcttccc ctgccgaaga gcccgagccc
      301 gcggtcgaga ttgccaccct ctcgcacctg gtcgccggtc cttcagcgcg cttcgccaag
      361 accgtccgcg acatcggccg gtccggttcg tgcaacatcg actacgcgtg cgtcccaagc
      421 ccgtcccagg cgctgcgcaa tgccgcgaat gccgtggtgc agatgctgtt caccgtgcgc
      481 agtggcggct ccttcctgtg caccggaacg ctcctcaacg acaccaaccc cggcacgcag
      541 gtcccgtacc tgttctcggg caaccactgc ttcgacgagg agttcgcgcc gttcaacaac
      601 gcgacgcaga tgcagcaggt ggcgaacagc ctcaacacat tcttcttctt cgacgccgtg
      661 gcctgcggga gcttgcaggc gccgccgttc gtgcagcgtt tcggcggagc gacttacctg
      721 tacagcaacc tggcccagga cgtgctgctc gtgaggctga acgactgggc gccggccggc
      781 gcgttccttt ccggatggga cccgaatccg ctttcggccg gaacttcggt gaccttgctg
      841 caccatccgg gtggcgacct gaagaaatac agcaccggca cgacgggcag cctcgtcacg
      901 ctggtcagcc cggtcgacgc cgtaacggga ttcattcccg tcacctacaa ccagggcacc
      961 gcggagcccg gctcgagtgg cgcaggcctg ctgacattct ccaatgccaa tggccagtac
     1021 ctggtgcgcg gcggcctgtg gggcggctcg gcgagctgca ccgcactttc gtcgctggac
     1081 tactactcgc gcttcgacgt cgcctatccg accctgcggc agtggctgga agccgcgaac
     1141 ctgcccgaat tcgacgtgac ggacctctgg tggaatccgt cggaggatgg ctggggcatc
     1201 aacctcaccc agcacccgag cgggcaggtg ttcgccgtct ggtacaccta cgccgccgac
     1261 tccggcccgc tgtggctggt gatgtccgcc gggcaatggg tgacgagcag gtccttcagg
     1321 ggcaagctct accgcacgag cggacccggc tatacccagc tgccgttcaa cccggccgcg
     1381 gtgaagcaaa acgaagtggg aacgctcacg ctcgatttca cgagcgccgg cagcggttcc
     1441 ttcacctggg tggtggacgg cgtgcagggc acgaaagtca tcacgcgcca ggcgttctga
     1501 acccggccgc ggctcgcgca taatgcgagc catggcgcct gcctccctct cgatcgccat
     1561 cgtcacgcac gcgcccgacg agcgcctgct cgcgcgcacg attgcgcgcg ccgccgacgc
     1621 gatggccttc gcccgcgagc gcggctcgct gggttccgcc cgcctgcaga tcgtcgacaa
     1681 cggccccggc gacctcgcga ccatgctcgg gcaactgggc gaggccgccc tcgcccgcgt
     1741 ggagagggcc gaactggacg tgatctccgg ccagggcaac gtgggattcg ggcggggcca
     1801 caacctcgcc atcgcccgtt cggcggccga cttccacctc gtgctgaatc cggacgtgga
     1861 gatggaccgt gacgcgctcg atgcggcact tcgctacctg gacgcgcatc ccggcgtcgg
     1921 cgccatcacg ccttcggtgc gcgggaccga cggcgagcgc gaatacctgg tgaaggcctg
     1981 gcccacgccc ggcgtgctct tcgttcgggg attcgtgccg cggttcctgc acggtttgtt
     2041 ccgccggcaa ctggacgcct acgagctgcg gcacctggac tgggagaagg agcagtcccc
     2101 cgtcgcgatc gccagcggct gcttcctgct ttgccgaggc gcggcgcttg ccgcggtgaa
     2161 gggtttcgac cccggcttct tcctctattt cgaggacttc gacctcacgt tgcggctttc
     2221 ccgcgtgacc acggtggcgt actgcccgca ggtgcggatc gtgcatcacg gcggcaaggc
     2281 ggcggcgaag ggatggcggc acatcgcgtg gttcgtgaac tcggcacggc ggttcttcac
     2341 cctgcacggg cgcgctcccg ccggctgatc cgccgcgcgc gtcttcgcgc gccgcggaaa
     2401 tcgctctttc gattttcgcg attctcgcgg gcgcggcaat gctcgctttc ctagcatggg
     2461 ctccagtccg gtcacgcccc gcgcagaccg gcagccaaag gaggagccca tgaaagagct
     2521 gaccgaacac gaagtcctcg ccgttgccgg cggcctgcct tccggctccg cgctcgacga
     2581 agtgtcctac cgcgcgcccg ccgagccgct tcgcgatccg ctggcgttcg ccgaactcgc
     2641 gaccgaggaa catccggatc gggtcaccga ttgacccggg gcgccgcgcc tcgccggtcg
     2701 cccgcgccgc gccgacattt ccgcgaccgc agcccaagga gattgccatg aaggacatcg
     2761 acaagaagga ctcgccggat gtctcgggcg gagtttcgcc cgacgacagc ggatgcttgc
     2821 cgcgtttccc gttttccatc gactatccgc ccaatccgga catgccgttc ccggagccgc
     2881 cgcgcagctt cgacgacccg gtaccggtcg tcaacgtgaa gtgaatcgca ggatcaacgc
     2941 aaggagaatg ccatgagcga accggatgtt ccgaaggctt tggagaagaa cgaattgagt
     3001 gggacgcagg tggaggacat cgcaggaggg gggctaatcg acggccagac cctaatcgac
     3061 ttctcggcct cgctgatcca gacctacgac aacctcgtcg cctttgcctc gcacgtgatc
     3121 gggagggtcg cgggcgaacc ctgagcgcac cttacgtgtc gtgacgatcc caagggctgc
     3181 gctgccgcag cccttctcga cgagaccttc tcatgcccgc gcccaccggt cttcagattg
     3241 cattcctggc cttcgccttc gaattctttt ctatgctcgc atcgaagacg ctggccccgc
     3301 tcatccattg gccggccgac cagttcgcgt tgctgggcca actgatctcg ttctcgatgg
     3361 cgacgctgat cctgttcggc ttccccgggt tgcgcaagcg ctgtctctcc gaacttcgcg
     3421 ttccggttcc cgcagggtcg tcgccggaac tgatcgccgc ctccattgct catctggcag
     3481 ttccgttcgc agtcgtcggc ggtgtcgtca tgcaggcctt cgcaacggga agcgcggacc
     3541 agcttctatc gagaattccc accaatgacc cttcgatggc gtgggaagac acgctctcgc
     3601 cccccggcct tcttcggctg ttcctcctgg cgtggttcgt gggaccggta atcgaggagc
     3661 tcgtctttcg cggatttctc tatcgtgcct tcgagcgcca gtgggggtgg ccggtgtcga
     3721 tggcgctcac gtctttcctg ttcggtttgg ctcacccgac acacatcgcg gctgcggcgc
     3781 tgggctcggt catcctgatc tgcgttcttc gtcgaaccgg ttcccttcgg gcgtgcattt
     3841 tcgtccacat cacgttcaac attctcatct cctggcctct gttcggacag gtgctgctca
     3901 cggcgccgaa aggcctttca accagcattg ccacctggtc cattcaactg gcttgcctcg
     3961 cctttgccgc ggtggcactg cccgcgtacg tgtggatgtc gcgcaaggcg aaacttcccg
     4021 ccggcgacgg cgaagtcgcc gcatctccgg ttcaccttgg ctcttcccgc atttcgtcct
     4081 gaggttcacg ccgaacaggc ccgttcggcg ctcgggacca tcgtattgat ccggccgctg
     4141 tcctacgcgg cgctgacgat tgtcgcggtc gccatcgccg tcgcgatcct gtcctatctc
     4201 ttcatcgccg aatacgcgaa gaaggcatcc ctcgcaggca ccctcgttcc cgcatccggc
     4261 gcgatccggg tcgtggcgca gcagcccggc ctcgtgcgcg agcgccgcgt gagcgaaggc
     4321 gagcgggtgg ccgcagggga agcattgctg cgcctcgtgg acgcgcgcgc cacgcgcgat
     4381 gacggtcccg tgggcgaagc cacggtcgcc ctggcggaaa cacggatcgc acagacgcgc
     4441 cggcagcgcg tcgaaatcaa ggccgccacg cacagcgagc gcgacgccct tcgcggcagg
     4501 atcgcgggcc tcgaagagga actgactcac ctcgacggcg aactcgatgc cctcgtcgcc
     4561 cgcgaggcac tcgcgcaacg ctcgctgcaa cgcttcgacg acctgcagcg gcgcggcttc
     4621 gtgtcgccgg cgcagcgcca gcagaaggag gaggagtcgc tggagcggcg ctcgcggcgg
     4681 cacgccgcgg cccgggcgcg gctgggtctc gagcgcgagc ttgcaggcct tcgccttgcc
     4741 gtcaccgagt cggaggcgcg ctcgcgcgcc cagctctcgg cgctcgacgc acagctcgcc
     4801 gccctcgcgc aggaacgggt cgagcggcgg gcgcaggccg atgcggtggt gaccgcgccc
     4861 gccgcgggaa ccgtggcggc actgctcgtg gagccggggc aggtggtcgg atccggggcc
     4921 aacctgctca cgctccttcc ggacggctcg cccctggaag cgcacctctt cgcgccgtcg
     4981 cgcgccatcg gattcatccg ggcaggccag gaagtgatgc tgcgttttcc ggcgtttccc
     5041 taccagaaat tcggcagcca tcgcgcccgc gtgctttcgg tttcgcgaag cgccctcgcc
     5101 cccgcggagc tgggctttgc gccgcccgat ggatcgcgcg agccgctcta ccgcgtcaag
     5161 gtggcgctcg ggacgcagac cgtggccgcc tacggccgcg ccgagcccct gcaggccggc
     5221 atgcaggtag agggcgacgt gctcctcgac aggcgccgcc tcatcgagtg ggtgttcgag
     5281 ccgcttttca gcctggccgg aagggcctga agtggggccg cttgcggggc tcgacttcgg
     5341 cggcgcccgg cggcttcccg tcttcctgca gacggaggcc gccgagtgcg ggcttgcgag
     5401 cctggggatg gtcgcgagct tccacgggca ccgcatcgac ctcgccgcgt tgcgccggcg
     5461 cttcacgctt tccctcaagg gcgccacgct cgcctacctg atgcaggtgg ccggcaggct
     5521 gcacctcgcg ccgcggcccc tgcgcctgga tctggacgag ctgccgcaac tgaagacccc
     5581 gtgcatcctg cactgggatc tcaaccactt cgtcgtcctg aaatcggccg cggcgcgcga
     5641 ggtggtcatt cacgatccgg ccttcggcgt gcggcgcctg ccgatggccg aggtgtcgaa
     5701 gcatttcacg ggaatcgcgc tcgagctcgt ccccaccgcc gagttccggc ccgccgacga
     5761 acgccgccgc gtgcgccttc gcgacctcac ggggcccgtc gcggggctgg cgcggtcgct
     5821 cgcgcaggtg ctcgcgctcg ccctcgtgct ccagtcgttc gcggtcctcg ctcccttcta
     5881 catgcaatgg gtggtcgacg gcgccgtcgt gtcggcggac cgcgacctgc tcgccgtgct
     5941 cggcctggga tttctcctcc tcgcgctggt ccaggtggcg gtgacggccc tgcgttcgtg
     6001 ggtcgtgctc catctcggca cgatcctcaa catccagtgg ctcgccaacg tgttctcgca
     6061 cctgctgcgc ctgcccgtcg cgtggttcga gaagcgccac ctcggcgacg tggtgtcgcg
     6121 cttcggcgcg gtgacgacga tccagcgcac gctcacgtcg agcttcgtcg aggcggtgat
     6181 cgacgggctg atggcggtgg cgacgctggc gatgatgctc gtctacagcg gcgcgctcac
     6241 ggcgctcgcg gtgatcgccg tggccgccta cgcggtctcg cgggccgcgt tctatgcgcc
     6301 gctgcgccgc gcgacggagg agagcatcgt ccatgccgcg cgccagcaaa gccacttcct
     6361 cgagacggtg cgcggcgtgc agtcgatcaa gctcttcggg cggcaggagg agcggcgcgc
     6421 gcggtggctc aacctggtcg tcgatgcggt gaaccgcgat ctcgtcgtcc agaagctctc
     6481 gctcggattc cgctcggcca acggcctcgt cttcggcgtc gagcgcatcg ccgtggtctg
     6541 ggtgggcgcg ctcctggtgc tcgactccgc cttttccatc gggatgctct tcgccttcat
     6601 ggcctacaag gagcagttct cggcgcgcgt ggccgggctc atcgacaagc tgatcgagct
     6661 gaagatgctg caactgcagg gcgagcgcct ggccgacatc gtgctgacgc cgcccgagga
     6721 ggaggcacgc tcgcccgcgc ccgtcgtgga ggagacttcc atcgaggtga ggaacctctc
     6781 gttccgctat tccgacaccg agcccttcgt gctgcagaac tgcgcgtttc gcatcgagcc
     6841 cgcggagtcg gtcgcgatcg tgggcccctc gggctgcggc aagacgaccc tggtgaagat
     6901 catgctgggt ctgctcgcgc ccaccgacgg gcaggtgctg gtcggcggca tcgacctgca
     6961 gaggctgggc gtcgaaccct accgccgcct cgtcggaacg gtgatgcagg acgatctcct
     7021 gttcgccggt tccatcgccg acaacgtgag cttcttcgac cccggcccca gccaggaggc
     7081 gatcgagcgc tgcacgaagc tcgccgccgt gcacgacgac atcgccacga tgcccatggg
     7141 ctaccacacg ctcatcggcg acatgggcgc ggcactgtcg ggcgggcagc ggcaacgcat
     7201 ccttctcgcg cgcgcgctct acaagcagcc gcgcatcctc ttcctcgacg aggcgacgag
     7261 cgcgctcgac gtgcagcgtg aacgccaggt gaacgcggct atccggggcc tcaacctcac
     7321 gcgaatcctc atcgcgcacc ggcccgagac gatcgcctcg gcggggcgcg tcatcgtgct
     7381 gcagggcggc aaggtcgcgc aggacttgcg gcgggcggtc gatacgtcgc acggatcggg
     7441 ggacgcggcg acacacgcgg cttgagcagc atggaatcga cacctgtcca ttaattatgt
     7501 tgacatgtca tctgctaagg ggcgagaatg ccgcatgatc catacacgca agcgcggccg
     7561 cccccggact tcggacctca cccgggccga gcagcttcgc gctgcgaaac gcgcccagcg
     7621 ccgtcgcgag cgcgaagcgg gtatcgcggc ggtggaattg cgcctgccaa gcgaaagggc
     7681 gacccagctt cgcgcagcga cggccgcccc gcgattcaag gccttgctcg gcagcttgct
     7741 cgataacctg gttctcgatc tcgaggcctg gcctgtcctg agggagctcg cctggagccg
     7801 gtcgggccgc tggatccttg cggaagatgc gttggccatc tacgaacgca attggcgctt
     7861 cgtcgaccct cgccatctcg gcaaggacga agcggcgctc atcgagcggc tgaaggcgac
     7921 ctgcggcgcg ggggtcctca atgcataacc ggcatttcga gcgcccggcc cacggcgagg
     7981 ttgtcgcgct gctcgcttca agatacttcg cgaactgcgc catcgagctc gccggaagct
     8041 acggagagga agtggagcgc gagctggcat cggtgacggc caggctgcgc gaggacaagg
     8101 cttaccgcaa c
//
LOCUS       DASUEV010000105        10248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1519051, whole genome shotgun sequence.
ACCESSION   DASUEV010000105 DASUEV010000000
VERSION     DASUEV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519051"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VFV55_03450"
     CDS             <1..1291
                     /locus_tag="VFV55_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEX4943382.1"
                     /translation="EDDGDARPRERTAATIAKARQQASVGMLTDALGDESFCHALVEA
                     IGDGGEFATALGRLRFVPTGSYAALAGAAPGKLPVSAPIAQSSNTAVTLGERLFLKCY
                     RRLRRGINPEFEIGCFLTNVARFPNCVPVAGGVEHVAADGSTSALAVLQAYVSNQGDG
                     WNYTLEYLDRHFADTGSAGEGTHGPYLALSRTLGERVAQLHRAFATPGGGADFDPEPV
                     TERDVAAWKARARAEAQATLELLSRRAGVLDDVVRHEAGMLLANRRGLLATIDGAVIA
                     TKGMCKLRYHGGLHLGRVLLRNNDFLIIDFEGETACAPEERRAKHSPLRDVAGLLRSF
                     DYARWSALRRPPHGAQEYARCAPLADAWHTAARQAFLEGYAQAVEGAGILASLEAARG
                     LVRLFEIEKALCELRQELENRPAWAAIPLNGLRALAG"
     gene            1323..2003
                     /locus_tag="VFV55_03455"
     CDS             1323..2003
                     /locus_tag="VFV55_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943383.1"
                     /translation="MNVDLLVEPVQVLLRQAGEFLPRLALAALVMIAGWLAAKLVRLA
                     VVRGLRAVNFNVLTERAGMDGFLKQGGIETDTTGIFGVLAYWLIILASLVIAFNGLGL
                     TYITDLLGKVVLFLPKVIVALLVLAFGTYFAGFIGHAVTAYCRNIGVADADLLGRIAQ
                     YALIVFVVLIALDQTNIGGDIVRQSFLILLAGAVLGISLAFGLGGRRWAARLLERWWP
                     SERDKDGS"
     gene            complement(2027..4384)
                     /gene="parC"
                     /locus_tag="VFV55_03460"
     CDS             complement(2027..4384)
                     /gene="parC"
                     /locus_tag="VFV55_03460"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX4943384.1"
                     /translation="MLDTKTPDLFDSAPPAPPPQRPAGGASSGDGDVPLATFAESAYL
                     AYAMSVVKGRALPAIEDGQKPVQRRILYAMRELGNRHDAPFKKSARIVGDVIGKFHPH
                     GDSAVYEAAVRMAQDFTLRYPLIQGQGNFGSRDGDGAAAMRYTEVRLTAFAEAILLSE
                     LERGTVDFIPTYDGSLEEPRLLPARLPIALLNGASGIAVGMATEIPPHNMGEVVEACV
                     VALGSAKAADAQILASIPGPDFPGGGQVISPPEEIAKAYETGRGSVRVRARWTVEKLA
                     RGQYRVAVTETPPGTNAARVLAEIEEITNPKPRAGRKTVSAEAQALKNAVLGMLESAR
                     DDSDREHPVRLVFEPRSSRIAPEELMNLLLAHTSLESNAPMNLVAIDREGRPRQAGLA
                     HWIREWAAFRLEALERRLRHREGEVAERLHILDGRMIAFLNIDKVIKVIRGADEPRPA
                     LMEKFALSERQAEDILEIRLRQLAKLEGIRIEREIGELKSERAQLAKLLASEAARRKL
                     AVKEVTADGGRFGDRRRTTIEAAERAAAAPVETLSDEPITVVCSRNGFLRTRTGHGID
                     AAALSWKEGDGPLAILETRTIHPIVLVGENGRTYSVRALDIPGGKGDGVPATSLVDLA
                     GTRIMGVLAGDPVTQVLLASSGGTGLRCRLGDLFTRQRAGKAFMNVGEGEKCLAPETI
                     REGATEVATLSAGGRLLVFPLAEVNELSGGGKGVILMRLHEGEALLGARVSSGALKVK
                     GKGRGDKVSFVTVSPSDLETYRGNRARAGRVLQGTLKSFAGFADA"
     gene            complement(4405..6372)
                     /locus_tag="VFV55_03465"
     CDS             complement(4405..6372)
                     /locus_tag="VFV55_03465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX4943385.1"
                     /translation="MSSRYDERSIRVLKGLEPVKERPGMYTRTDTPLHIIQEVVDNAA
                     DEALAGAARTITVTLHADHSVSVEDDGRGIPVGPHPEEKVSTVEVVFTRLHAGGKFDK
                     KVGGSAYAFSGGLHGVGVSVTNALSKRLEVRVKRDGAAWALAFEDGFLVEKLRKTGTV
                     PAKQTGTSVRAWPDAKYFDSDRIKRAELEHLLRAKAVLLPGLTMRLTDEATGATTEWK
                     YEGGMAAYLQESLAGEEGVASPVIVGESYCGEGDSQFAQGEGAAWALAWCEGGGGGES
                     FVNLIPTVDGGTHESGLKSGVFDSIREFCLHHELLARGVTLQTEDVWKYLRFVLSVRL
                     LDPQFQGQTKAKLNSRDAVKLVSLRLKDPLDAWLNQNVEAGKKIAELAIRAAVARQKA
                     GKATEKRRGSGVAVLPGKLTDCESEDIARNELFLVEGDSAGGSARQGRDRHFQAILPL
                     RGKILNTFEVDPTQIYANNEVHDIAVALGIEPHGIGEAAKALSGLRYGKVVIMADADV
                     DGSHIQTLLLALFFRHFPGLLAHGHVFIAQPPLYCVKVDAQGKSRPERRLYALDEAER
                     DAILLKLEDEKVREGAVHVGRFKGLGEMNPEELRETTMSPDTRRLLPLTVSQEILADT
                     HKVFTLLMGKGEAASRRNWMEERGNLVEADI"
     gene            complement(6428..7498)
                     /locus_tag="VFV55_03470"
     CDS             complement(6428..7498)
                     /locus_tag="VFV55_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4943386.1"
                     /translation="MKKPILLVAAALSAAAAFAPWAQKGGAPQPRAVAPRGPLLPQEQ
                     SLANLFDSAAPSVAYITTERLERTGLFTAEVAQGAGSGFVWDAEGHVVTNYHVLAGAR
                     SVAVSLDSGKPIEATVAGFSEDYDLAVVKLDKVPKHLKPIPLGASRDLRIGQTVYAIG
                     NPYGLTRTLTTGIVSALDRHLPTSEYREIAGAIQTDAAINPGNSGGPLLDSAGRLIGV
                     NTAIRSPSGGSSGVGFAIPADLVNRIVPQLIARGKAQLPGIGIRAVDPVLAARAGITG
                     VVVAEVLRGSPASEAGLVAVDRRTGEVGDVIVAVNGRPVEQMGTFAAELDRAGIDNVA
                     ELTVVREKKERKVRVKVVDVQR"
     gene            complement(7566..8180)
                     /locus_tag="VFV55_03475"
     CDS             complement(7566..8180)
                     /locus_tag="VFV55_03475"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX4943387.1"
                     /translation="MQLIGMLDSPYVRRVAISMKLMEIPFEHRPLSVFRHYERFRDIN
                     SVVKAPSLVCDDGVVLMDSTLILDYLEGLVAPERRLMPLRGDARREALRLTGLALAAC
                     DKGVSLVYEREQRPPERRHEPWYERVLAQTLAAFTALEDATGKADPWLQGTGPNAADI
                     AVAVAWRFGQYYNAQGVPAAKFPALVKFSAQAEALPAFQSTPLD"
     gene            complement(8188..8469)
                     /locus_tag="VFV55_03480"
     CDS             complement(8188..8469)
                     /locus_tag="VFV55_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943388.1"
                     /translation="MNGFVALAQDAAGGRRENGEAASFTMNLDHIRLQLDELEHRAEE
                     TRDPMEMRRINLECTALERSIVIVEEEFERNIAMLEAVRKRLRRLRARA"
     gene            complement(8501..9937)
                     /locus_tag="VFV55_03485"
     CDS             complement(8501..9937)
                     /locus_tag="VFV55_03485"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX4943389.1"
                     /translation="MYPIPQFVRHAGRALAAVLAASVIMSPTQGGGGVTGTGFVAYGE
                     VTDFGSVFVNGIEFFTQSANVIIDGVSNRSESELRIGMPVRVDGVLNPDGRTGNASTV
                     TYSADLRGIVDGPATSSDDEGALFFIHGLAVRVDEYTVFESLAGPRSLAAGDRVEASG
                     LRDDETGELYATYVSRGTLSAGTVVSGAISETGASSFRLGSLTVQYDAMALRNVPSGE
                     LANGMVVRVRADAAPVAGNLQATDVSVELESLAAVPGTKGSAEGIVSLPTPAGFWLGS
                     LMVLVDERTQFRDGTAAGLLDGALVGVEGTVQAGGALLAERVSYVNLMNASAQAVVAA
                     TDATGFRLVSAEGVRVNVNSSTRFIDRSTEDPSQFGPGDLRVGDTVRVTGLEAAAGTI
                     LAERVMRIRPTNVVELRARSRSAVSPTAVLLAMNAVTGPSTVFRDEKGEEISQQAFFA
                     KALGRQVRAKGLVQGSDSFVAATIDIES"
     gene            complement(9960..>10248)
                     /locus_tag="VFV55_03490"
     CDS             complement(9960..>10248)
                     /locus_tag="VFV55_03490"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEX4943390.1"
                     /translation="NVPVESLPVLRAFAREKGEQVLDELAREMSRHDRDVNPAVGGTG
                     RTRAVVGIYFAEEVLSREYEPAQVATDEDAKRTDQPAKARGRKRPKIGSPE"
BASE COUNT         1486 a         3599 c         3503 g         1660 t
ORIGIN      
        1 ggaggacgac ggcgacgcgc gcccgcgcga gcgcaccgcg gcgaccatcg ccaaggcgcg
       61 acagcaggcc agcgtcggca tgctcaccga cgcgctgggc gacgagtcgt tctgccacgc
      121 cctcgtcgag gcgatcggcg acggcggcga attcgccacc gcgctcggca ggttgcgctt
      181 cgtgccgacc ggctcctacg ccgcgctcgc gggcgctgcc ccgggcaagc tgccggtatc
      241 ggcgccgatt gcgcagagct cgaacaccgc ggtgacgctc ggcgagcggc tgttcctcaa
      301 gtgctaccgc cggctgcggc gcggcatcaa cccggaattc gagatcggat gcttcctcac
      361 gaacgtcgcg cgcttcccga actgcgtgcc ggtggcgggc ggcgtcgagc atgtggctgc
      421 cgacggctcg acgagcgccc tcgcggtgct gcaggcctac gtctcgaacc agggcgacgg
      481 ctggaactac acgctcgagt acctcgaccg gcacttcgcg gacacgggtt cggcgggcga
      541 aggcacgcac ggtccgtacc tcgcgctctc gcgcaccctt ggcgagcggg tcgctcaact
      601 ccaccgggcc ttcgcgacgc ccggcggcgg cgccgacttc gatcccgagc cggtgacgga
      661 gcgcgacgtg gcggcgtgga aggcgcgcgc acgcgccgag gcacaggcca cgctcgaact
      721 cctgtcgcgc cgggccggag tcctcgacga cgtcgtgcgc cacgaggcgg ggatgctcct
      781 cgcgaaccgg cgcgggctcc tcgccacgat cgacggcgcc gtcatcgcga ccaaggggat
      841 gtgcaagctg cgctaccacg gcggactgca cctcggccgg gttctgctgc gcaacaacga
      901 cttcctcatc atcgacttcg agggcgagac ggcgtgcgcg cccgaggagc ggcgcgcgaa
      961 gcactcgccg ctgcgcgacg tggcggggct gctgcgctcc ttcgattacg cgcgctggag
     1021 cgcgctgcgg cgcccgccgc acggggcgca ggaatacgcg cgctgcgcgc cgctggccga
     1081 cgcctggcac accgcggcac ggcaggcgtt cctcgagggc tacgcgcagg ccgtggaggg
     1141 cgccgggatc ctcgcgtcgc tcgaggcagc gcgcggcctg gtccggctct tcgagatcga
     1201 aaaggcgctc tgcgagctgc gccaggagct ggaaaaccgg cccgcgtggg ccgccattcc
     1261 gctcaacggg ctgcgtgcgc tcgccggctg aacgcgggga cgaaagacga agggagaatg
     1321 cgatgaacgt ggatctgctg gtcgaacccg tacaggtact cctcaggcag gcgggcgaat
     1381 tcctgccgcg gctcgcgctc gccgcgctgg tgatgattgc cgggtggctc gcagccaagc
     1441 tcgtgcggct cgccgtggtg cgggggctgc gcgccgtcaa cttcaacgtg ctcaccgagc
     1501 gcgccggcat ggacgggttc ctgaagcagg gaggcatcga gaccgacacc accggcatct
     1561 tcggcgtgct ggcgtactgg ctcatcatcc tcgcctcgct ggtgatcgca ttcaacgggc
     1621 tgggcctcac ctacatcacg gacctgctcg gcaaggtcgt gctcttcctg cccaaggtca
     1681 tcgtcgcctt gctggtcctc gccttcggca cctacttcgc cggcttcatc ggccacgccg
     1741 tcaccgccta ctgccgcaac atcggcgtgg ccgacgcgga cctgctcgga cgcatcgccc
     1801 agtacgcact catcgtcttc gtggttctca tcgccctcga ccagacgaac atcggcggcg
     1861 acatcgtgcg ccagagcttc ctcatcctgc tggcgggggc cgttctcggg atttcgctcg
     1921 ccttcggcct cggcgggcgg cgctgggcgg cgcggctact cgagcgctgg tggccctcgg
     1981 agcgcgacaa ggacggcagc tagcgccgcg gtcgcggcgc tgcagcctac gcgtcggcga
     2041 agccggcgaa ggacttcagc gttccctgca gaacccggcc cgcgcgggcg cggtttccac
     2101 gataggtttc gagatccgac ggcgacacgg tcacgaagga caccttgtcg ccgcggccct
     2161 tgcccttcac cttgagggca ccggaggaga cccgggcgcc gaggagcgct tcgccctcgt
     2221 gaaggcgcat gaggatcacg cccttgccgc cgccggaaag ctcgttcacc tccgcgagcg
     2281 gaaagaccag gagccggccc ccggccgaga gcgtcgcgac ttccgtggcg ccttcccgga
     2341 tcgtttcggg cgcgaggcat ttctcgcctt cgcccacgtt catgaacgcc ttgccggccc
     2401 gctgccgcgt gaagagatcc cccagccggc agcgcaggcc ggttcccccg ctggaggcca
     2461 ggagcacctg cgtcacggga tcgcccgcga gcacgcccat gatgcgcgtg ccggccaggt
     2521 ccacgagcga ggtcgcggga acgccgtcgc ccttgccgcc ggggatatcc agcgcccgca
     2581 cgctgtaggt tcgcccgttt tcgcccacca gcacgatcgg gtggatggtc cgcgtctcga
     2641 ggatggcgag cggcccgtcg ccctccttcc acgacagcgc ggccgcgtcg atgccatgtc
     2701 cggtgcgcgt gcgcaggaac ccgtttcggg agcagacgac cgtgatcggc tcgtcgctga
     2761 gcgtctccac gggtgccgcg gcggcacgct cggcagcctc gatcgtcgtg cggcgccggt
     2821 cgccgaagcg cccgccgtcg gccgtcactt ccttcaccgc gagcttgcgc cgggctgcct
     2881 cgctggcgag cagcttcgcg agctgcgccc gctcggactt gagctcgccg atctcgcgct
     2941 cgatgcggat gccctcgagt ttcgcgagct ggcgcaggcg gatctccagg atgtcctcgg
     3001 cctgccgctc ggagagcgcg aacttctcca tgagcgcggg cctgggctcg tcggcacccc
     3061 ggatcacctt gatcaccttg tcgatgttga ggaaggcgat catgcggccg tcgaggatat
     3121 gcaggcgctc ggcgacctcg ccctcccggt gcctcaggcg ccgctccagc gcctccagcc
     3181 ggaatgccgc ccactcgcgg atccagtgcg cgagcccggc ctgccgcggc cgcccttcgc
     3241 ggtcgatggc caccaggttc atcggggcgt tcgattcgag cgaagtgtgc gcgagcagca
     3301 ggttcatgag ctcttcgggc gcgatgcgcg agctgcgcgg ctcgaacacc agccgcacgg
     3361 gatgctcgcg gtcggagtcg tcccgcgcgc tctcgagcat gccgagcacg gcgttcttga
     3421 gcgcctgcgc ctcggccgac accgttttcc tgcccgcgcg cggcttggga ttggtgatct
     3481 cctcgatctc ggccagcacc ctggccgcgt tggtgcccgg cggcgtctcg gtcaccgcga
     3541 cgcggtactg cccgcgggcg agcttctcca ccgtccagcg cgcgcgcacc cggacgctgc
     3601 cgcgccccgt ctcgtaggcc ttcgcgatct cttccggcgg cgagatgacc tgcccgccgc
     3661 ccggaaaatc gggacccggg atggaagcga ggatctgcgc atcggcggct ttcgcgctcc
     3721 ccagcgcgac cacgcacgcc tcgaccacct cgcccatgtt gtgcggcggg atctcggtgg
     3781 ccatgcccac ggcgatgccg gatgcgccgt tgagcagcgc gatgggaagc cgcgcgggca
     3841 ggagcctggg ctcctcgagc gagccgtcgt aggtcgggat gaagtcgacg gtgccgcgct
     3901 cgagctcgga gagcaggatc gcttccgcga atgcggtgag ccgcacttcg gtatagcgca
     3961 tggccgcggc gccgtcgccg tcgcgcgagc cgaagttgcc ctgcccctgg atgagcggat
     4021 agcgcagcgt gaaatcctgc gccatgcgca cggccgcctc gtacacggcg gagtcgccgt
     4081 gcggatggaa cttgccgatc acgtcgccca cgatgcgcgc ggatttcttg aacggggcat
     4141 cgtggcgatt gccgagttcg cgcatcgcgt agaggatgcg ccgctgcacc ggcttctggc
     4201 cgtcctcgat ggccggaagc gcgcggccct tcaccacgct catcgcgtag gcgaggtagg
     4261 cgctctcggc gaaagtcgcg agcggcacgt cgccgtcgcc ggaggaagcg ccgccggccg
     4321 gccgctgcgg cggaggcgcg ggcggggcgg agtcgaacag atcgggagtc tttgtatcga
     4381 gcatgatttg aacggggaca gtccctagat gtccgcctcg acgaggttgc cgcgctcttc
     4441 catccagttg cggcgcgagg cggcttcgcc cttgcccatg aggagggtga agaccttgtg
     4501 cgtatcggcg aggatttcct gcgagaccgt gagcggcagg aggcgacgcg tgtccgggct
     4561 catggtcgtt tcgcgaagct cctccgggtt catctcgccc aggcccttga agcggcccac
     4621 gtggacggcg ccttcgcgca ccttctcgtc ctcgagcttc aggaggatcg cgtcgcgctc
     4681 cgcctcgtcc agcgcgtaca ggcgccgctc ggggcggctc tttccctggg cgtcgacctt
     4741 gacgcagtaa agcggcggct gcgcgatgaa gacgtgcccg tgggcgagca gtcccgggaa
     4801 gtgccggaag aagagcgcca gcagcagcgt ctggatgtgc gagccgtcca cgtccgcgtc
     4861 ggccatgatg acgaccttgc cgtagcgcag cccggaaagc gccttcgcgg cctccccgat
     4921 gccgtgcggc tcgatcccga gggcgaccgc gatgtcgtgc acctcgttgt tggcgtagat
     4981 ctgcgtgggg tcgacctcga aggtgttgag gatcttgccg cgcaagggca ggatcgcctg
     5041 gaagtgccgg tcgcgcccct gccgggccga gccgccggcg gaatcgccct cgacgaggaa
     5101 aagctcgttg cgcgcgatgt cctcgctctc gcaatcggtg agctttccgg gcagcaccgc
     5161 cacgccggag ccgcgccgct tctcggtggc cttgccggcc ttctgccgcg caacggccgc
     5221 ccgtatcgcc agctcggcga tcttcttgcc ggcctccacg ttctggttca gccaggcgtc
     5281 cagcgggtcc ttgaggcgca gggagacgag cttcaccgcg tcgcgggaat tgagcttcgc
     5341 cttcgtctgc ccctggaact ggggatccag gaggcgcacg gaaaggacga agcgcaggta
     5401 cttccacacg tcctcggtct gcagcgtaac gccgcgcgca agtagctcgt ggtgcaggca
     5461 gaactctcgt atcgaatcga acacgccgga cttgaggccg gattcgtgcg tgccgccatc
     5521 gacggtgggg atgaggttca cgaaggattc gccgcccccg ccgccttcgc accaggcgag
     5581 cgcccaggcg gcgccttcgc cctgcgcgaa ctgggagtcg ccctcgccgc agtacgactc
     5641 gcccacgatg acggggctcg ccacgccttc ctcgccggcc aggctctcct gcaggtaggc
     5701 ggccatgccg ccttcgtact tccactcggt ggttgcgccc gtggcttcgt cggtgagccg
     5761 catggtgagg ccgggaagca gcaccgcctt ggcgcgcagc aggtgctcga gttcggcgcg
     5821 cttgatccgg tcgctgtcga agtacttggc atccggccag gcgcgaaccg aggttccggt
     5881 ctgtttcgcc gggaccgtgc ccgtcttcct gagcttctcg acgaggaagc cgtcctcgaa
     5941 cgccagcgcc caggcggcgc cgtcgcgctt cacgcgcacc tcgaggcgtt tcgacagcgc
     6001 gttggtgacg gagacgccta ccccgtgcag gccgccggag aaggcgtagg cggacccgcc
     6061 caccttcttg tcgaactttc cgcccgcgtg caggcgcgtg aagacgacct cgacggtgga
     6121 caccttctcc tcggggtgcg ggccgacggg gatgccgcgg ccgtcgtctt ccacgcttac
     6181 ggagtggtcg gcgtgaagcg tcacggtgat cgtcctggcg gcgcccgcca gcgcctcgtc
     6241 ggccgcgttg tcgacgacct cctggatgat gtgcagcggg gtatcggtcc gggtgtacat
     6301 ccccgggcgt tccttgacgg gctcgaggcc cttgaggacg cggatggatc tttcgtcgta
     6361 acgggaggac atgcgggggg ccggctttcg aatccgctat tttacggtgt ccgacccctt
     6421 ttccgctcta gcgctggaca tcgacgacct tgacccgcac cttgcgttcc tttttttcgc
     6481 gcacgacggt gagctcggcc acgttgtcga tgccggcgcg gtccagctcc gccgcgaagg
     6541 tgcccatctg ctcgaccggc cggccgttca cggcgacgat cacgtcgccc acctcgcccg
     6601 tgcggcggtc caccgccacg agcccggctt cggaagcggg cgacccgcgc agcacttccg
     6661 ccacgaccac cccggtgatg ccggcgcgcg cggcgagcac gggatcgacc gcccggatgc
     6721 cgatgcccgg cagctgcgcc ttcccgcgcg cgatgagctg cggaacgatg cggttcacga
     6781 ggtcggcggg gatcgcgaag cccacgccgg aggaacctcc cgatggcgag cgtatcgccg
     6841 tgttcacgcc gatgagccgc ccggcgctgt cgagcagcgg cccgccggag ttgcccgggt
     6901 tgatcgccgc gtcggtctgg atggcgcccg cgatctcgcg atattccgaa gtcggcaggt
     6961 gccggtccag cgcgctcacg atgcccgtgg tcagcgtgcg cgtcagcccg tacggattgc
     7021 cgatggcgta caccgtctgc ccgatgcgca ggtcgcggga agcaccgagc ggtatcggtt
     7081 tcaggtgctt cggcaccttg tcgagcttca cgacggcgag gtcgtagtcc tccgagaatc
     7141 ccgccacggt ggcctcgatg ggcttgcccg aatcgaggct caccgcgacg ctgcgggcac
     7201 cggccagcac gtggtaattg gtgaccacgt ggccttccgc atcccagacg aagccgctgc
     7261 ccgcgccctg cgccacctcg gcggtgaaca gcccggtgcg ctccaggcgc tcggtggtga
     7321 tataggcgac cgatggggcc gcggaatcga aaaggttcgc gagcgactgc tcctgaggca
     7381 ggagagggcc cctgggcgct accgcccggg gctggggggc gccgcctttc tgcgcccagg
     7441 gcgcgaaggc ggcagcggcc gaaagcgcgg cggcgaccag caggatcggc ttcttcatgg
     7501 agggcattcc ggaaaggtgg acccccggaa ggtcgtggcg gttccccgcc gcttcaaggg
     7561 gccgttcagt cgagcggcgt ggactggaag gccggcaacg cctcggcttg cgcggaaaac
     7621 ttcaccagcg ccggaaattt cgccgccggc acaccctgcg cgttgtagta ctgcccgaaa
     7681 cgccaggcca ccgcaactgc gatgtcggcc gcgttcggcc cggtgccctg aagccagggg
     7741 tcggccttgc cggtggcatc ctccagcgcc gtgaaggccg cgagcgtttg cgccaggacg
     7801 cgctcgtacc agggttcgtg gcgcctttca gggggacgct gctcgcgctc gtacaccagg
     7861 gacacgccct tgtcgcaggc cgccagtgcg aggccggtga gccgcagcgc ctcgcgccgc
     7921 gcatcgcccc gaagcggcat gaggcgcctc tccggcgcaa cgagtccctc gaggtagtcg
     7981 aggatgagcg tggaatccat gagcacgact ccgtcgtcgc agacgagcga cggcgccttt
     8041 accacggagt tgatgtcacg gaagcgctcg taatggcgaa acacggaaag cggcctgtgc
     8101 tcgaagggta tctccatgag cttcatggag atggccacgc gccggacgta gggtgaatcg
     8161 agcatgccga tgagctgcat gacggcctca ggcccgcgcc cgcaggcggc gcaggcgctt
     8221 gcggaccgct tccagcatcg cgatgttgcg ctcgaattcc tcctcgacaa tgacgatgga
     8281 acgctccagc gccgtgcatt ccaggttgat gcggcgcatc tccatcgggt cgcgggtttc
     8341 ctcggcccgg tgctcgagtt cgtccagctg caggcgaata tggtccaggt tcatggtgaa
     8401 tgaggccgct tccccattct cccgccgccc gcccgcggca tcttgcgcca gcgcaacgaa
     8461 cccgttcatc ggcgcgtgac cggcgcgaac cccgcccccg ctacgattcg atatcgatgg
     8521 tcgccgcgac gaaggaatcg gatccctgca ccagcccctt cgccctgacc tggcggccca
     8581 gggctttcgc gaagaacgcc tgctggctga tctcctcgcc cttctcgtcc cggaagaccg
     8641 tggaagggcc cgtcaccgcg ttcatcgcga gcaggaccgc ggtcgggctc accgccgatc
     8701 gcgaccttgc acggagttcg acgacattcg tgggacggat gcgcatgacg cgctcggcga
     8761 ggatggtgcc cgcggcggct tccaggccgg ttacgcggac cgtatcgccc acgcgcaggt
     8821 caccaggacc gaattgggaa gggtcttcgg tgctgcggtc gatgaagcgc gtcgaggaat
     8881 tcacgttcac gcgcactccc tcggcggaaa cgagccggaa acccgtcgcg tccgtcgcgg
     8941 ccaccaccgc ctgcgcggat gcattcatca gattgacata cgagacgcgc tcggcgagga
     9001 gcgcgccgcc cgcctgcacg gtgccctcga caccgaccag cgcgccgtcg agaaggccgg
     9061 ccgcggtccc gtcgcgaaat tgcgtgcgct catcgacgag caccatcagg ctacccagcc
     9121 agaacccggc aggcgtgggc agggacacga tgccttcggc gctccccttg gtacccggga
     9181 cggccgcaag gctttccaat tcgacgctca cgtcggtcgc ctgaaggttg cctgccaccg
     9241 gggcggcatc cgccctcacc ctcacgacca ttccgttcgc gagctcgccg gatgggacgt
     9301 tgcgcagcgc catcgcgtcg tattggacgg tgagcgagcc cagcctgaag ctcgaggcgc
     9361 ccgtttccga aatcgctccc gagaccacgg tgcccgccga aagcgtgccg cgcgaaacgt
     9421 aggtcgcgta gagttcgccc gtctcgtcgt cgcgcagccc gctagcctcg acgcgatcgc
     9481 ccgccgcgag cgagcggggt ccggccaggg actcgaagac cgtgtactcg tccacgcgca
     9541 ccgcgaggcc gtggatgaag aagagcgcgc cttcgtcgtc cgaactcgtg gcaggcccat
     9601 cgacgattcc gcgcaggtcg gccgagtacg tcacggtgga ggcgttgccg gtccttccgt
     9661 ccggattcag gacaccatcg acgcgcaccg gcatgccgat gcgcagctcg ctttcgctgc
     9721 ggttcgatac gccgtcgatg atcacattgg cggactgcgt gaagaactcg atgccgttca
     9781 cgaaaacgct tccgaaatcg gtcacctcgc cgtacgcgac aaagcccgtg cccgtcacgc
     9841 cgccgccgcc ctgcgttggc gacatgatca cggaagccgc gaggaccgcg gcgagcgccc
     9901 ggccggcatg gcgcacgaat tgggggatgg ggtacacgat tattctcctg actcttatgt
     9961 cattcgggcg acccgatctt gggtcgcttc ctgccgcgcg ccttcgccgg ttgatccgtg
    10021 cgcttcgcgt cttcgtcggt cgccacttgc gccggttcat actcccggga caacacttcc
    10081 tcggcgaaat agatccccac gacggcccgc gtccttccgg ttccgcccac ggcgggattc
    10141 acgtcgcgat cgtgacgcga catctcgcgg gccagctcgt cgagcacctg ttcgcccttc
    10201 tcccgcgcga acgcgcgcag cacgggcagg ctttcgacgg gcacgttg
//
LOCUS       DASUEV010000106         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_831998, whole genome shotgun sequence.
ACCESSION   DASUEV010000106 DASUEV010000000
VERSION     DASUEV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831998"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFV55_03495"
     CDS             <1..309
                     /locus_tag="VFV55_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX4943391.1"
                     /translation="KDDPKKPAAAAEMKAVALLDLNTASREELVKLKGIGDARADAII
                     KGRPYKGKNELVDKKIVPENVYNDIKDKVIAKQADTTPAKAVKAEAKPAEGKKKKGGC
                     "
     gene            complement(402..1958)
                     /locus_tag="VFV55_03500"
     CDS             complement(402..1958)
                     /locus_tag="VFV55_03500"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX4943392.1"
                     /translation="MQDQLIIFDTTMRDGEQSPGASMTRDEKVRIGKQLERLRVDVIE
                     AGFAAASDGDFEAIRAVAEAVKDSTVCSLARANDKDVRRAGEAVAPASRKRIHTFIAT
                     SPIHMKLKLRMSEDQVLEAAVKAVKLAREYTDDVEFSAEDAVRSEVDFLCRIFEATIA
                     AGANTINVPDTVGYSVPERWGALFRELLTRIPNAGKAVWSTHCHNDLGMAVANSLAAV
                     TNGARQVECTINGLGERAGNASLEEIVMAVRTRSDVYSCRTGIDATQIVPTSKLVSTI
                     TGYPVQPNKAVVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWGANRLTLGKLSGR
                     NAFRSRLTELGITLASEEALNAAFAKFKELADKKREIFDEDLQALVSDESVTPEQERL
                     KLVSLVAHSETGEQPFARVVITDAGHEMRSEARGAGPVDASFQAIEDMVHSGTELLLF
                     SVNNITTGTDSQGEVTVRLAKDGRVVNGMGADTDIVVASAKAYLSALNKMGISAARVN
                     PQYGEESVTP"
     gene            complement(2165..2617)
                     /locus_tag="VFV55_03505"
     CDS             complement(2165..2617)
                     /locus_tag="VFV55_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4943393.1"
                     /translation="MSWVIQRWRTESKEKQYQSIRDAIDEGDVTALARMLHTTAPDTV
                     VNGEGRSLLMVAVEESNVPAIELLLARGANPNHRDGQGSTALALASRMGFQEAVEVLL
                     SAGADPNVRDVSGMTALDIAEEHGSHDIVAALLRIGGQSGRDLPPAQP"
     gene            complement(2603..3328)
                     /gene="pssA"
                     /locus_tag="VFV55_03510"
                     /pseudo
     CDS             complement(2603..3328)
                     /gene="pssA"
                     /locus_tag="VFV55_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
     gene            complement(3338..3985)
                     /locus_tag="VFV55_03515"
     CDS             complement(3338..3985)
                     /locus_tag="VFV55_03515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX4943394.1"
                     /translation="MAHYPHPVIAREGWPFLAGSLALAALSTWLGGWWSLPFWLVAAF
                     VLQFFRDPAREVPAVEGAVLAPADGRIVQVGKARDDYLGRDAVKISVFMNVFNVHSNR
                     SPVDGTVANVWYVPGLFVNADLDKASTGNERNAVHLRTASGMDVTVVQVAGLIARRIL
                     CYVGQGDRLSRGQRYGFIRFGSRVDVYLPPGSTPKVAIGDKVSATSTVLALLPAA"
     gene            complement(4023..>4535)
                     /locus_tag="VFV55_03520"
     CDS             complement(4023..>4535)
                     /locus_tag="VFV55_03520"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076384.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX4943395.1"
                     /translation="CAIGGTRGGVIETNFREETETDLFGEQTVLCGGIVELIKAGYET
                     LVEAGYAPEMAYFECLHETKLIVDLIYEGGIANMNYSISNNAEFGEYLTGPKVIGPES
                     RKAMKEALAHIQSGGYARDFILENRAGAPTLLARRRLLAEHPIEQVGEKLRAMMPWIR
                     KNKLVDQSRN"
BASE COUNT          749 a         1518 c         1529 g          739 t
ORIGIN      
        1 aaggacgacc cgaagaagcc cgccgccgca gccgagatga aggccgtggc cctgctcgat
       61 ctcaacaccg cttcccgcga ggaactggtc aagctcaagg gcatcggcga cgcgcgcgcc
      121 gacgcgatca tcaagggccg cccgtacaag ggcaagaacg aactcgtgga caagaagatc
      181 gttcccgaga acgtctacaa cgacatcaag gacaaggtca tcgccaagca ggccgacacc
      241 acgccggcga aagccgtgaa ggcggaagcc aagccggccg aaggcaagaa gaagaagggc
      301 ggttgctgac ctcccggccc cgcggtcgcg accgcggggc cggaggcatg cggtgaagat
      361 gcccgcgatg agctcgcggg catcttcatt gaattccggc ttcagggagt caccgactcc
      421 tcgccgtact gcgggttcac gcgcgcggcg ctgatgccca tcttgttgag ggcgctcagg
      481 taggccttgg cggaggcgac gacgatgtcc gtgtcggcgc ccatgccgtt caccacccgg
      541 ccgtccttgg cgagccgcac ggtcacttcc ccctgggagt ccgtgccggt ggtgatgttg
      601 ttcaccgaga aaagcagcag ttccgtcccg ctgtgaacca tgtcctcgat cgcctggaac
      661 gaggcgtcga cggggccggc cccgcgcgct tcggagcgca tctcgtggcc tgcgtcggtg
      721 atcaccacgc gcgcgaacgg ctgttcaccg gtctcggagt gggccaccag ggagaccagc
      781 ttcaggcgtt cctgctcggg cgtgacgctc tcgtcggaga cgagcgcctg gagatcctcg
      841 tcgaagattt cgcgcttctt gtcggccagt tccttgaact tggcgaacgc cgcattcaac
      901 gcctcctccg aggcgagcgt gatcccgagt tcggtcagcc ggctgcggaa ggcgttgcgc
      961 ccggacagct tgccgagcgt gaggcggttc gcgccccagc ccacgtcctc ggcacgcatg
     1021 atctcgtagg tctcgcggtg cttgagcacg ccgtcctggt ggattccgga ttcgtgcgcg
     1081 aaggcgttgg cccccaccac cgccttgttg ggttgcaccg gatagccggt gatcgtcgac
     1141 accagcttgc tggtcggcac gatctgcgtg gcatcgatcc cggtgcggca ggagtagacg
     1201 tcggaccggg tcctcaccgc catcacgatt tcctcgagcg aggcgttgcc ggcgcgctcg
     1261 cccaggccgt tgatcgtgca ttcgacctgg cgcgcgccat tggtcaccgc ggcgagcgaa
     1321 ttggccaccg ccatcccgag gtcgttgtgg cagtgggtgg accagaccgc cttgccggca
     1381 ttcgggattc gcgtgaggag ctcgcggaaa agcgcgcccc agcgctcggg cacgctgtag
     1441 ccgacggtgt ccggcacgtt gatcgtgttc gcgcccgcgg cgatggtggc ctcgaagatg
     1501 cggcagagaa aatcgacctc cgagcgcacc gcgtcctccg ccgagaactc gacgtcgtcc
     1561 gtgtattcgc gcgcgagctt caccgccttc acggcggctt ccagcacctg gtcctcgctc
     1621 atgcgcagct tcagcttcat gtggatgggg ctggtggcga tgaaggtgtg gatgcgcttg
     1681 cgcgatgccg gcgccaccgc ctcgcccgcg cgccggacgt ccttgtcgtt ggcgcgggcg
     1741 agcgagcaca cggtcgaatc cttcaccgct tccgcaacgg cgcggatggc ctcgaaatcg
     1801 ccatcgctcg cggccgcgaa gccggcctcg atcacgtcga cgcgcagcct ctcgagctgc
     1861 ttgccgatgc gcaccttctc gtcgcgggtc atggaagcgc ctgggctttg ctcgccgtcg
     1921 cgcatggtgg tgtcgaaaat gatcagctga tcctgcatgg ttcgctccgg aatgggggaa
     1981 ggttcacacg ccggcccgtt tgccgggcgg caccgcaact gccgaatggg ggggtgggat
     2041 atttagcgcg cgaggcgcag cagcaggccg gggaggcccg ccagggggat gcggagcagg
     2101 tggtcggcga ttcgcgcgac ggagttcatg gagcgactat agcaacggcc gcaggggctg
     2161 gcaactaggg ttgcgcgggc ggaaggtcgc gcccggactg cccgccgatg cgcaggaggg
     2221 ccgccacgat gtcgtgcgaa ccgtgctcct cggcaatgtc cagcgccgtc attccggata
     2281 cgtcgcgcac gttggggtcg gcaccggcgg aaagaaggac ctccaccgcc tcctggaatc
     2341 ccatccggga cgccagggcc agcgccgtcg atccctgccc gtcgcggtgg ttcgggttcg
     2401 cgccccgggc gagcagcaac tcgatcgcgg gcacgttcga ctcctccacc gcgaccatca
     2461 gcagcgagcg cccttctccg ttcaccaccg tgtccggggc ggtcgtgtgc agcatgcgcg
     2521 cgagcgccgt gacgtctccc tcgtcgatcg cgtcgcggat gctctggtac tgcttctcct
     2581 tcgactcggt gcgccagcgc tggatgaccc agctcacgta gccggaaact ccgtaagcca
     2641 ccatgaggcc ccacagcacg tgcgacggct cgatcgagat gagcagcagg gcgaccacga
     2701 tcaccagcgt catccagaac gggacggcgc ggcgaaggtt gagctccttg ccgctgtaga
     2761 acctcacgtt cgagaccatg gtcacgccgg cataaacggt gagcgccgcg gcgaaccact
     2821 tcacctctcc gcctttcacg tggtaatcgt tgagcaccca gatcatgcct gccaccaggg
     2881 ccgcggcggc cgggctgggc agcccggtga accagcgctt gtcggccacg gaaagctggg
     2941 tgttgaaccg cgcgaggcga agcgcggcgc cgacgcagta cacgaaggcc gcgatccagc
     3001 cgatgcgccc catcccccgc agcgcccact cgtacatcac gagcgccggc gccgcgccga
     3061 aggagaccat gtccgcgagg ctgtcgtatt ccgcgccgaa tgcggactgc gtgtgcgtga
     3121 ggcgcgccac ccgtccgtcg aggccgtcga ggaccatcgc cacgaagatc gcgatggagg
     3181 cgcgctcgaa ctcgtggttc attccctgca cgatcgcata gaagccggcg aagagcgcca
     3241 gcgtcgtcag gaggttcggc agcaggtaga tcccgcggcg gcgaacgggc tccatcaacg
     3301 tctggtggcg cttcgcggat gtgtccatgg cttcctttca ggctgcggga agcagcgcaa
     3361 gcaccgtgga cgtcgccgaa accttgtcgc cgatggcgac cttcggcgtc gatccgggcg
     3421 gaaggtacac gtcgacgcgc gagccgaagc ggatgaatcc gtagcgctgg ccgcgcgaaa
     3481 gccggtcacc ctgtcccaca tagcagagga tcctcctggc gatcagcccc gcgacctgca
     3541 ccacggtgac atccatcccg gaggcggtac gcaggtgcac ggcgttgcgc tcgttgccgg
     3601 tggacgcctt gtcgagatcc gcgttgacga aaagcccggg gacgtaccac acgttcgcga
     3661 ccgtgccgtc gacgggggag cggttcgagt ggacattgaa gacgttcatg aagacgctga
     3721 tcttcaccgc gtcgcgcccg aggtagtcgt cgcgcgcctt gcccacctgc acgatccgcc
     3781 cgtcggccgg cgcgaggacg gcgccctcga ccgcgggaac ctcgcgcgca ggatcgcgga
     3841 agaactgcag cacgaatgcc gcgaccagcc agaacggcaa cgaccaccat cccccgagcc
     3901 acgtggagag ggccgcgagg gccagggagc cggccaggaa gggccagccc tcgcgcgcga
     3961 tgaccgggtg cgggtagtgc gccaaggggg ccctcagccg ccggctcgtt gcgcagccac
     4021 gcctagttgc ggctctggtc gacgagcttg ttcttcctga tccacggcat catggcccgg
     4081 agcttctcgc cgacctgctc gatgggatgc tcggcgagca ggcgccggcg cgcgagcagg
     4141 gtcggggcgc ccgcgcggtt ctcgagaatg aagtcgcgcg cgtagccgcc ggactggatg
     4201 tgggcaagcg cctccttcat cgccttcctg gattcgggac cgatcacctt gggcccggtc
     4261 aggtattccc cgaactccgc gttgttggag atcgagtagt tcatgttggc gatgccaccc
     4321 tcgtagatga ggtcgacgat gagcttggtc tcgtgcaggc actcgaagta ggccatctcg
     4381 ggagcgtaac ctgcctccac cagcgtctcg tagcccgcct tgatgagttc gacgattccg
     4441 ccgcagagca ccgtctgctc gccgaagagg tcggtctccg tctcctcgcg gaaattcgtc
     4501 tcgatgaccc cgccgcgcgt gccgccgatc gcgca
//
LOCUS       DASUEV010000107         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_245444, whole genome shotgun sequence.
ACCESSION   DASUEV010000107 DASUEV010000000
VERSION     DASUEV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245444"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1009)
                     /locus_tag="VFV55_03525"
     CDS             complement(89..1009)
                     /locus_tag="VFV55_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX4943396.1"
                     /translation="MAFYAGARPGIGDFLRIVDIRTKIVSVSSLLVGSGWALVTVPDR
                     FSWGLFALMAAATLLIDMGTTGFNSYFDFVTGVDTRESDQERFKALVQSEIDPRIALH
                     LSFALYAVAMPLGLAIGTRAGWSVVAAGAFCMVFAFFYSGGPHALSRTPVGEFFAGGL
                     LGGALVAIAAFVHTQRLDGATLLVGLPSSLIIAGILSTNNACDRVGDARAGRRTLAIV
                     LGPERSHWPVFALVAAAFAAVALLAALRVLPPWAFLPMVPAAAFAWRTLAAMKARGWS
                     HATKAANMGGISLVFIAFTACLLASFALGA"
     gene            complement(1013..2350)
                     /locus_tag="VFV55_03530"
     CDS             complement(1013..2350)
                     /locus_tag="VFV55_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4943397.1"
                     /translation="MTILSDQIHLHPDQLDAFWMPFTANRQFKAAPRLMAKAEGMYYW
                     TPEGRKILDGFAGLWCCNAGHNRKPIVAAIQQQAATMDYAPAFQMGHPLAFELANRIV
                     DIAPEGMNHVFFCNSGSEAVDTALKIALAWHRVRGEGARTRLIGRERGYHGVGFGGIS
                     VGGMVANRKMFGTMLAGVDHLPHTHDLGHNAYSRGEPSWGAHFADELERLVQLHDAST
                     IAAVIVEPMAGSTGVLIPPKGYLKRLREITKKHGILLIFDEVITAFGRLGGAFSSDVY
                     GVVPDMITFAKGITSGSVPMGGVIASSEVHDAFMVGPPGAIELFHGYTYSAHVLACAA
                     GVATLQLYKDEDLFARARKMAPVLEDALHSLKGTRHVIDIRNTGLVGAVELEPRPGAP
                     GARTFDTYIGCWDKGVLVRQAGDIICLAPALVVEESHIAEMVGTLRAVLESVK"
     gene            2529..3467
                     /locus_tag="VFV55_03535"
     CDS             2529..3467
                     /locus_tag="VFV55_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4943398.1"
                     /translation="MRLVTYCAGPAGPARAGVRVGHRILDIEAASRVKGEPLPSSMKA
                     LFAAGRGALSRVQALAKAATSEARSFSQAMHEERAIRLLPPVPDADKFLCVGKNYRQH
                     LDELVKNDLLKEIPQEPTAFVKLNSSLVGNDAKVARPQGIVRLDYEPELVFVIGRRAL
                     GVKKIDAMDYVAGVTILNDLTCRDLQKKEVASGSRFWTGKNIPGFGPLGPEIVTMDEV
                     PDPYDLWMTCSVNGVERMRVNTRDQIWKLPDILEHFSRHIPVEPGDMFSTGAPGGVAV
                     GKPNAAELFLKPGDVVECSIEGIATLRTFIVAPGEA"
     gene            3467..>3855
                     /locus_tag="VFV55_03540"
     CDS             3467..>3855
                     /locus_tag="VFV55_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX4943399.1"
                     /translation="MAAGRRLILAALAALVLPPIPDAFAQSYPAKSVKIVVPFPPGGG
                     ADTLARIIGPHLAKIWGQPVVVENKPGASGHIGADMVAQSPADGYTLLMASTAALSEK
                     NVKNFAPVSLVSASAYVIVANPKVKAGT"
BASE COUNT          656 a         1309 c         1308 g          582 t
ORIGIN      
        1 gagcgacggc aatacgacag aaaagtaacc tgaccccttt tccgttttgc ctattttttg
       61 tgcaaagtaa cctgacccct tttcctgttc aagctcccag cgcgaagctg gcgagaaggc
      121 acgcggtgaa ggcgatgaac acgagcgaga tgccgcccat gttcgctgcc ttggtcgcgt
      181 gcgaccagcc gcgcgccttc atggccgcaa gggttcgcca ggcgaacgcg gcggcgggga
      241 ccatcggcag gaatgcccac ggcggaagca cgcgcaaggc ggcgagcaag gccaccgcag
      301 cgaaggctgc cgccacgagg gcgaacaccg gccagtgcga tcgttccggg ccgagcacga
      361 tggcgagcgt gcgccgcccc gcgcgcgcat cgccgacgcg gtcacacgcg ttgttggtgg
      421 aaaggatgcc ggcgatgatg agcgacgagg gcagccccac gagcagggtc gcgccgtcca
      481 gccgctgcgt gtgcacgaag gccgcgatgg cgaccagagc accgcccagc aacccgcccg
      541 cgaagaactc gcccacgggc gttcgcgaca gggcgtgcgg cccgccggaa tagaagaagg
      601 cgaagaccat gcagaaggcg cccgcggcca ccacgctcca gccggcgcgc gtgccgatgg
      661 cgagccccag gggcatcgcc acggcgtaga gggcgaagga gaggtgcagc gcgatgcgcg
      721 ggtcgatttc gctctgcacc agtgccttga accgctcctg gtccgattcg cgcgtgtcca
      781 ccccggtgac gaagtcgaag tagctgttga accccgtggt gcccatgtcg atgaggagcg
      841 tggccgccgc catcagcgca aagagccccc aggagaatcg atccggcacg gtcacgagcg
      901 cccagccgct gccgaccagc agcgaagaga cgctgacgat cttggtgcgg atgtcgacga
      961 tgcgcaggaa gtcgccgatg ccgggtcgcg cgcccgcgta gaacgccatc ccctacttga
     1021 cgctttccag caccgcgcga agcgtgccga ccatctcggc gatgtgcgat tcctcgacga
     1081 cgagcgccgg ggcaaggcag atgatgtcgc cggcctggcg caccagcacg cccttgtccc
     1141 agcagccgat gtaggtgtcg aaggtgcgcg cgcccggcgc gcccgggcgc ggctcgagct
     1201 cgaccgcgcc cacgagaccg gtgttccgga tgtcgatcac gtgccgggtg cccttgagcg
     1261 aatgcagtgc gtcctccagc accggcgcca tcttccgggc gcgggcgaaa aggtcctcgt
     1321 ccttgtacag ctgcagcgtc gccacgccgg ccgcgcacgc gaggacgtgc gccgagtagg
     1381 tgtagccgtg gaacagctcg atggcgcccg gcgggcccac catgaacgcg tcgtggacct
     1441 cggacgaggc gatgacgccg cccatcggca cgcttcccga ggtgatcccc ttggcgaagg
     1501 tgatcatgtc gggcacgacg ccgtagacgt cggaggaaaa ggccccgccc aggcgcccga
     1561 aagccgtgat cacctcgtcg aagatgagca ggatgccgtg cttcttcgtg atctcgcgca
     1621 gccgcttgag gtagcccttc gggggaatca gcacgccggt ggagcccgcc atcggctcga
     1681 cgatcacggc ggcgatcgtg gaagcgtcgt gaagctgcac cagccgctcc agctcgtccg
     1741 cgaagtgcgc gccccaggag ggctcgccgc gggagtaggc attgtggccg agatcgtgcg
     1801 tgtgcggcag gtggtccacg cctgccagca tcgtgccgaa catcttgcga ttggccacca
     1861 tgccgcccac cgagatgccg ccgaagccca cgccatggta gccgcgctcg cggccgatga
     1921 gccgcgtcct ggcgccctcg cccctcacgc ggtgccaggc cagcgcgatc ttgagcgcgg
     1981 tgtcgaccgc ctccgatccc gagttgcaga agaacacgtg gttcatgccc tcgggcgcga
     2041 tgtccacgat gcggttggcg agttcgaagg cgagcgggtg gcccatctgg aaggcgggcg
     2101 cgtagtccat cgtcgcggcc tgctgctgga tcgccgccac gatgggcttg cggttgtggc
     2161 cggcgttgca gcaccacagg cctgcaaagc cgtcgaggat cttccttcct tcgggcgtcc
     2221 agtagtacat gccctcggcc ttcgccatca gccgcggcgc cgccttgaac tggcggttgg
     2281 ccgtgaaggg catccagaag gcgtcgagct ggtcggggtg caggtggatc tggtcggaaa
     2341 ggatcgtcat ggcgggtccg gggcggctcg taagcgggaa accgtcattc tgcactcatg
     2401 cagacccgcc tcgccaacgc ggtcacgttc tccgcgccgg cctcgccttg ccccgcctcc
     2461 tgcgggggaa cacaatggaa cgctgaaggc accatgcatc catccggaaa catcagcggg
     2521 accattccat gagactcgta acatattgcg ctgggcccgc cggccccgcg cgcgccggcg
     2581 ttcgcgtcgg ccatcgcatt ctcgacatcg aagcggcgtc gcgcgtgaag ggcgagccgc
     2641 tgccttcgtc gatgaaagcg ctgttcgccg ccgggcgcgg cgccctgtcg cgcgtgcagg
     2701 cgctggccaa ggccgccacc agcgaggcgc gctcgttctc gcaggcgatg cacgaggagc
     2761 gcgccatccg cctgctgccg ccggtgccgg acgccgacaa gttcctctgc gtgggcaaga
     2821 actaccgcca gcacctcgac gagctggtga agaacgacct cctgaaggag atcccgcagg
     2881 aacccacggc cttcgtgaag ctcaattcca gcctcgtggg caacgatgcg aaggtcgccc
     2941 gtccgcaggg catcgtgcgc ctggactacg agccggagct ggtcttcgtg atcggccgcc
     3001 gcgccctggg ggtgaagaag atcgacgcga tggactacgt ggccggcgtg acgatcctca
     3061 acgatttgac ctgccgcgac ctgcagaaga aggaggtggc ctccggctcg cgcttctgga
     3121 cgggcaagaa catcccgggc ttcggcccgc tgggtcccga gatcgtcacc atggacgagg
     3181 ttcccgatcc ctacgacctg tggatgacct gctcggtgaa cggcgtcgaa cgcatgcgcg
     3241 tcaacacgcg cgaccagatc tggaagctgc cggatatcct cgagcacttc tcgcgccata
     3301 tccccgtcga gccgggggac atgttctcga ccggcgcgcc tggcggggtg gcggtgggca
     3361 agcccaacgc tgccgaactc ttcctgaaac cgggcgacgt cgtcgagtgc tccatcgagg
     3421 gcatcgccac gctgcgcact ttcatcgttg cacccggaga agcctgatgg ccgcagggcg
     3481 ccgattgatc ctcgccgccc tcgcggcgct cgtcctcccg cccatccctg acgcgttcgc
     3541 gcagtcatat cccgccaagt cggtgaagat cgtcgtgccc tttccgccgg gcgggggcgc
     3601 ggacacgctg gcgcgcatca tcggcccgca tctcgcgaag atctggggcc agccggtcgt
     3661 cgtggaaaac aagccgggcg ccagcggcca catcggcgcg gacatggtcg cgcagtcgcc
     3721 ggcggacggc tacacgctcc tcatggcctc gaccgccgcg ttgagcgaga agaacgtgaa
     3781 gaacttcgcg ccggtgtcgc tggtgtcggc ctcggcctac gtgatcgtcg ccaaccccaa
     3841 ggtgaaggcg ggcac
//
LOCUS       DASUEV010000108         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_604076, whole genome shotgun sequence.
ACCESSION   DASUEV010000108 DASUEV010000000
VERSION     DASUEV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604076"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFV55_03545"
     CDS             complement(<1..408)
                     /locus_tag="VFV55_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943400.1"
                     /translation="MSPWGAKASLVMLDAGFGILRAITPGKSPDDILIVGLDDAAFES
                     PDVDPVGPALRKLPDVLVRIARGRPRAIALHARLPRASQEAAAPGWDDALATALSVAQ
                     AAAPLAVGMSVDPRREVVPIHDALLRGVDTRALV"
     gene            complement(589..1128)
                     /locus_tag="VFV55_03550"
     CDS             complement(589..1128)
                     /locus_tag="VFV55_03550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4943401.1"
                     /translation="MNHCNVCGAKAEYRVPPGDTHARAVCPACGHIQYQNPKIVVGSV
                     PVWADRVLLCRRAIEPRLGNWTLPAGFMENNETAPEGAAREALEEANARIEVEQLYTL
                     FSVPHISQVYVIFRAKLLDLDFSPGVESLEVKLVREEEIPWDELAFASVRRTLSHFYE
                     DRKSGVFLPRFGEIRPPER"
     gene            1225..2175
                     /locus_tag="VFV55_03555"
     CDS             1225..2175
                     /locus_tag="VFV55_03555"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX4943402.1"
                     /translation="MLVGALLSGTASGAEFGPDEMVIVLNSGDGSVSVVDPVRRKEVR
                     RIPVGKEPHHLMPTPDGRELIVANAASNDLVFLDAKSGEIRRRVENISDPYQLGYSPD
                     RKWFVSASNRLDRVDIYHASDFRLAKRIPLSKVPSHIAFSADSSTAFVSLQDSNEIAA
                     IDLDKQVVIWKMSTGSQPAGLWMTPDDRHLLVGMTGESHVEVYDWRARKLVKRLLTGQ
                     GAHNFVARGDGRHVFLSNRMANTISMIDQQTLEVKDTFAVPGGPDCMEITRDGKQLWT
                     TSRWTRMLTVVDLDTKKVVHQILVGKSPHGLYFQSHASRQ"
     gene            2172..2915
                     /locus_tag="VFV55_03560"
     CDS             2172..2915
                     /locus_tag="VFV55_03560"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX4943403.1"
                     /translation="MNVRAILVATLATATTAVAAPCAGTVYLTFDTGNMEPAEAILGV
                     LDRQGVKATFFLANEKTRRGDTSLDPSWAPFWKRVAEAGHAFGSHTWRHWYFAGDTAA
                     GKVRYAMPGSTQGEVLDAQALCAELKKPEEAFRAMTGRGFDGLWRAPGGRFTPMSVEY
                     ARRCGYTHAGWTPAGFSGDELPSDRYPGDKLIERQLRDIKDGDILLWHLGIWSRQDPL
                     WPRLDGLIAGLKAKGLCFARLTDDKRWIH"
     gene            2903..3844
                     /locus_tag="VFV55_03565"
     CDS             2903..3844
                     /locus_tag="VFV55_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX4943404.1"
                     /translation="MDPLIILWAKLHAAVFESVVQPIVFNLGMVSWLESSFEATELFL
                     LGAVQVILIALVLSALERWRPVEIWTDRRAIGVDIIYTLLMRLGILPLAIFFLLLPLV
                     GGLDGWLRLHDFIPPKLEDAFPSLFGHPFVSFVAYLVIIDFVLYWLHRGQHQIGIWWA
                     LHSLHHSQREMTFWTDDRNHLLDTALIQGALALSALVIGVAPGQFVAIIAVTRIIESF
                     SHANVRLAYGRIGELFLVSPRYHRVHHAIGFGHEGARMGANFAILFPVWDHIFGTASA
                     VRDYTATGIRDQLHGRNYGQGFWQQQWLGFKRMTGRA"
     gene            3841..>4386
                     /locus_tag="VFV55_03570"
     CDS             3841..>4386
                     /locus_tag="VFV55_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943405.1"
                     /translation="MSAARRCALALLAVPLLVVAAPEMPGRQWVAPQAQAAPLRVTEA
                     AATYRWDDALPKSTPAGEGPRALVGFVRASPKDFADVGWQAVDGGWAARFDVVSAGAE
                     GLRVRLDLSGADTLDVRVRDDAGRVESMTVPAGAGVAWGPWTEGAVQAVEVFSPVLPA
                     RGALRLGAVVHFDQPLDAKAAG"
BASE COUNT          708 a         1481 c         1457 g          740 t
ORIGIN      
        1 aacgagcgca cgcgtatcga ctccgcgcaa caaggcatcg tggataggca ccacctcgcg
       61 gcgcggatcc acgctcatgc ctaccgcgag cggtgcagcg gcctgggcca cggaaagcgc
      121 cgtcgcaagc gcgtcgtccc atcccggtgc agccgcctcc tgcgaggctc gcggcaaccg
      181 cgcatgcagg gcgatggctc gcggccggcc gcgcgcgata cgcaccagca cgtcggggag
      241 cttgcgtagc gcgggaccca ccggatccac gtccggcgac tcgaaggcgg cgtcgtcgag
      301 acccacgatg aggatatcgt cgggcgattt cccgggcgtg atcgcgcgca ggatgccgaa
      361 gcccgcgtcg agcatcacga ggctcgcttt cgcaccccag ggggacaaat ggaaagcgag
      421 gcccgcaagc gccaacgcag ccgtcgtcag gaaggcaaga agcgaaggaa aaggggtcag
      481 gttacttttc tgtcgtattg ccgtcgctag acggtgggct gaaaagtaac ctgacccctt
      541 ttcccatttt gcctattttt tgtgcaaagt aacctgaccc cttttccgtt agcgttcagg
      601 cgggcggatc tcgccgaagc gcggcaggaa cacgccggac ttgcggtctt cgtagaagtg
      661 cgacagcgtg cggcgaacgc tcgcgaaggc gagttcgtcc caggggatct cctcctcgcg
      721 caccagtttc acctcgaggc tttccacgcc cggcgagaag tcgaggtcga gcagcttcgc
      781 gcggaagatc acgtagacct ggctgatgtg cggcaccgaa aagagcgtat aaagctgctc
      841 gacctcgatg cgcgcgttgg cttcctccag tgcctcgcgg gccgcaccct cgggcgccgt
      901 ctcgttgttc tccatgaagc ccgccggcag cgtccagttg cccaggcgcg gctcgatggc
      961 gcggcggcac aggaggacgc ggtccgccca caccggcacg gaaccgacga cgattttcgg
     1021 gttctgatac tggatgtggc cgcaggcggg gcagacggca cgcgcgtgcg tatcgcccgg
     1081 cgggacgcga tactccgcct tcgcgccaca gacgttgcag tggttgatgg gaacgggggc
     1141 gcgggtcacg cgcttattcc agaaggacgg cgcagcattg agtgatggta tcataagcct
     1201 atgaaaaggc taggaaaaac aactttgctg gtcggcgccc tcctgtcggg cactgcaagc
     1261 ggggccgaat tcggccccga cgagatggtc atcgtcctca attccggcga cggcagcgtc
     1321 tccgtcgtgg atcccgttcg caggaaggaa gtcaggcgca tcccggtcgg caaggagccg
     1381 caccacctga tgccgacccc ggatggccgc gaactcattg tcgccaacgc ggccagcaac
     1441 gacctcgtgt tcctcgacgc caagtcgggc gaaatccgcc gccgcgtcga gaatatcagc
     1501 gacccctacc agctgggcta ttcgcccgac cgcaagtggt tcgtctcggc ttcgaaccgg
     1561 ctcgaccgcg tggacatcta ccatgccagc gacttccgcc tggccaagcg cattcccctg
     1621 tccaaggttc ccagccatat cgccttttcc gccgacagtt cgacggcctt cgtctcgctg
     1681 caggactcca acgagatcgc ggcgatcgac ctcgacaagc aggtcgtgat ctggaagatg
     1741 tccacgggca gccagccggc cggcctctgg atgacgcccg acgaccggca cctgctggtc
     1801 ggcatgacgg gcgaaagcca cgtcgaagtc tatgattggc gcgcccgcaa gctggtgaag
     1861 cgactgctca ccgggcaggg ggcgcacaat ttcgtcgcgc gcggcgacgg gcgccacgtc
     1921 ttcctgtcca accgcatggc caacacgatt tcgatgatcg accagcagac gctcgaggtg
     1981 aaggacacgt tcgcggtccc gggcgggccg gactgcatgg aaataacccg cgacggcaag
     2041 cagctgtgga ccacctcgcg ctggacccgc atgctcaccg tcgtggacct cgacacgaag
     2101 aaggtcgtgc accagatcct ggtcggcaag tcaccgcacg gcctgtattt ccagtcccac
     2161 gcttcgcggc aatgaacgtc cgggcgatcc tcgtggcgac ccttgccacg gcgacgaccg
     2221 ccgtcgccgc cccttgcgcc ggcaccgtgt acctcacctt cgacaccggc aacatggaac
     2281 cggcggaggc catcctcggg gttctcgacc ggcagggcgt gaaggccacg ttcttcctcg
     2341 ccaacgagaa gacgaggcgc ggcgacacct cgctcgaccc gtcgtgggcg ccattctgga
     2401 agcgcgtggc ggaggcgggc catgccttcg gctcgcacac ctggcggcac tggtatttcg
     2461 cgggtgacac cgcggcgggc aaggtgcgct atgccatgcc cggctcgaca cagggcgagg
     2521 tgctcgacgc gcaggccctg tgcgcggagc tgaagaagcc cgaagaggcg ttccgggcca
     2581 tgaccggccg cgggttcgac ggcctgtggc gagccccggg cgggcggttc acgccgatgt
     2641 ccgtggaata cgccaggcgc tgtggctaca cccatgcggg gtggacgccc gcggggttca
     2701 gcggcgacga gctgccctcc gaccgctacc cgggcgacaa gctgatcgag cggcagctcc
     2761 gcgacatcaa ggacggggat atcctgttgt ggcacctggg aatctggtcc cggcaggacc
     2821 cgctgtggcc caggctcgac ggcctcatcg ccggcctcaa ggcgaagggc ctgtgcttcg
     2881 cgcggctcac ggacgacaag cgatggatcc actgatcatc ctttgggcga agttgcacgc
     2941 cgccgtcttc gagtcggtgg tgcagccgat cgtgttcaac ctcggcatgg tctcctggct
     3001 cgagtcgtcg ttcgaggcca ccgagctgtt cctcctcggc gccgtgcagg tcatcctgat
     3061 cgccctggtg ctctcggcgc tcgagaggtg gaggcccgtc gagatctgga cggatcgccg
     3121 tgccatcggc gtggacatca tctacacgct gctcatgcgc ctgggcatcc tgccgctggc
     3181 gatcttcttc ctgctcctgc cgctcgtggg ggggctggac ggctggctgc ggctgcacga
     3241 tttcattccg cccaagctcg aggatgcctt cccgtcgctt ttcgggcacc cgttcgtctc
     3301 cttcgtcgcc tacctggtga tcatcgattt cgtcctgtac tggctgcatc gcgggcaaca
     3361 ccagatcggc atctggtggg cgcttcattc cttgcaccac agccagcgcg aaatgacgtt
     3421 ctggactgac gatcgcaacc acctgctcga cacggccctc atccaggggg ccctggcgct
     3481 ctcggcgctc gtcatcggcg tcgcgcccgg acagttcgtc gcgatcatcg ccgtgacacg
     3541 catcatcgaa agcttcagcc acgccaacgt ccgcctcgcc tacgggcgga taggcgaatt
     3601 gttcctcgtg agcccgcgct accaccgcgt gcaccatgcc atcgggttcg ggcacgaggg
     3661 cgcgcgcatg ggcgccaatt tcgccatcct cttcccggtc tgggatcaca tcttcgggac
     3721 ggccagcgcc gttcgcgact atacggcgac ggggattcgc gaccagttgc acgggcgcaa
     3781 ctacggccag ggcttctggc aacagcaatg gctcggcttc aagcgaatga ccgggcgcgc
     3841 atgagcgcgg cccgacgctg cgcgctggcc ctgctcgccg ttccgttgct cgtcgtcgcg
     3901 gcaccggaga tgccgggccg ccagtgggtt gcgccgcaag cccaggccgc tccccttcgc
     3961 gtgaccgagg cggcggccac ctatcgctgg gacgatgccc ttcccaagtc gacccccgcc
     4021 ggcgaggggc cgcgcgcgct cgtcggcttc gtgcgcgcct cgcccaagga tttcgccgac
     4081 gtcggctggc aagcggtcga tggaggctgg gcggcgcgat tcgacgtggt atccgcgggg
     4141 gccgagggcc tgcgcgtgcg cctcgacctt tccggtgcgg acacgctcga cgtgcgcgtg
     4201 cgcgacgacg ccggccgcgt ggagtcgatg acggttccgg cgggtgccgg cgtggcgtgg
     4261 ggcccctgga ccgaaggggc ggtgcaggcc gtcgaggtgt tctcgcccgt gctgcccgcg
     4321 cgtggcgcgt tgcggctggg cgcggtggtg catttcgacc agcccctgga cgccaaggcg
     4381 gcgggt
//
LOCUS       DASUEV010000109         5508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_833247, whole genome shotgun sequence.
ACCESSION   DASUEV010000109 DASUEV010000000
VERSION     DASUEV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833247"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFV55_03575"
     CDS             <1..791
                     /locus_tag="VFV55_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4943406.1"
                     /translation="TGYPGTAHRVNYVHNLFQNGAATLSGLVEMYHERVRRGELPDSK
                     DITFVMISGDGGMDIGMGPAIGAANRNHRMMILEYDNQGYMNTGAQLSYSTPFGHRTS
                     TSEVGAVLPGKRYHHKDTAQIFAACHVPYVFTASEGYPEDLMRKVAKAQWYAKNEGLV
                     YGKILSYCPLNWKTADDAAQPVLQAAIDCCFFPLYEIEHGHTTLTYDPDGSDRRHPVS
                     DWLKLMGKTRHLLKPANAAIVADIQAETDRRWARIKAMHEHPLL"
     gene            808..1656
                     /locus_tag="VFV55_03580"
     CDS             808..1656
                     /locus_tag="VFV55_03580"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX4943407.1"
                     /translation="MAKIIETRRLTPVTKYFRIEAPLIASSARPGQFVMLRVRDGGER
                     IPITIADYDRQAGTITIVVQEVGATTKRVCALEAGDEILDVAGPLGGHIDIAPGGHVC
                     GIGGGFGSAALLCLMREFTARGDTTTAILGARNKELLILADELRPLCTHLEICTDDGS
                     AGFKGFVTQRLKQLIDGGGERRPDSVVAIGPMAMMRAVAETTRAAKVKTLVSMDPLMV
                     DGTGMCGGCRVTVAGKAQFACVDGPCFDAHEVDFDVAMRRNKAYVQEEKQALDRVACM
                     ERREAP"
     gene            1656..3062
                     /gene="gltA"
                     /locus_tag="VFV55_03585"
     CDS             1656..3062
                     /gene="gltA"
                     /locus_tag="VFV55_03585"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX4943408.1"
                     /translation="MPIKRSDKTPMPVREAQMRARDFIEVNEGYTKSHASFEAERCLR
                     CQDPVCVGGCPVNVPIPEFIHAVAMGDMAGAARILRSANPLPAICGRVCPQESQCEAD
                     CSMTTRFTPVAIGHLERFVADWERTQPPSVEAKITRKEKIAVIGAGPSGLVCAGALAR
                     LGYPVTIYEALHAAGGVLRYGIPEFRLPKQVLDWEIGLLKSMGVEIVCNVIIGKTLTL
                     DELFGKMGFAAVFIGTGAGLPQFLGIPGENLNGVMSANEFLTRINLMRGYEFPESDTP
                     VRVGKRVAVIGAGNTAMDAVRTSLRMGAEEAMIVYRRSDKEMSARVEEYHHAQEEGVQ
                     FHWLTNPLEVVGNPEGWATGLKCQKMKLGEPDASGRARPVPIPGSEFVIPVDNVILAI
                     GTTPNPLLTRTTSGLLTNPKGCLIADESTGLTSKPLVFAGGDAVTGAATVILAAGAGK
                     RAGQAIHEALVERSRVPA"
     gene            complement(3059..3619)
                     /locus_tag="VFV55_03590"
     CDS             complement(3059..3619)
                     /locus_tag="VFV55_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX4943409.1"
                     /translation="MLEPIGSIEEFLAHALAIEQEAARSYREFQAHFNDHGEEVLAGL
                     CGNIAHFEQEHYELLLERTKGRTLPALTPERYRWLESGPPESAAHELVYRVATPRQLL
                     EIALKAERNARRFFEWVAGTTADGEVHALATEMAREESEHVQWVTQALEYARADAVEW
                     DKLLAAGGGPGLALGGERRVRRNTRA"
     gene            complement(3703..>5508)
                     /locus_tag="VFV55_03595"
     CDS             complement(3703..>5508)
                     /locus_tag="VFV55_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943410.1"
                     /translation="STRGVANLGAALAQFGLEAWALRNAQESYDPFWAGSHFFLADRY
                     PSRYARNSELFQGFLADPTALGGSNRFQALVPKPGAYATLAWRGATGDDVRLVEPALT
                     ANGLAADGRLAWFAEGARLRQWRTDGTQEDRATSTTFGIGAKLRHDIGFFAYGNRLVP
                     DVRAGADDPRRNFSIIDGTARRVDAGMGWRQSADRLFWVKVGSGDEESRIRANVAPAA
                     GTAQALVVQGSDFISRPERRDAQARALVRFEGGHVLSLGYESARWKSVDVLERDAFAH
                     APAATGLIESVRQDIRDDSRVVALAGHAQLGMATIEVQVDRTEYDKTNGIVVRRDFAD
                     QLVLLADNHARDEVNGRAGLEWRALPGTTLRLAWQQWLRPASIGSLSATATAGIMVDD
                     RFVLPGGRLEQARAQVEWEAGGGLLVTAHAQRQEIDNLYSALIGVLNNRPDSSNLERL
                     RNRSFNNLATLDQLEGFPDLSRGTLREAGAAVNYIVNRHLSLYAEGAWSDSENTLAYP
                     GKRFAYLPEKGGALGATFYSDHRFSIGAKAIYRGERFRDEANTPANRLRPEWDGTLQA
                     YWETQDKRWSVELLVSRIGAKSVDESFAIAVNARF"
BASE COUNT          906 a         1877 c         1902 g          823 t
ORIGIN      
        1 cgaccggcta tccggggacg gcccaccgcg tgaactacgt ccacaacctc ttccagaacg
       61 gcgcggcgac cctctccggc ctcgtcgaga tgtaccacga gcgcgtccgg cgcggcgagc
      121 tccccgactc gaaggacatc acgttcgtga tgatctccgg cgacggcggg atggacatcg
      181 gcatggggcc cgcgatcggc gccgcgaacc gcaaccaccg gatgatgatc ctcgagtacg
      241 acaaccaggg ctacatgaat accggcgccc agctctcgta ctcgaccccg ttcggccacc
      301 gcacctccac cagcgaggtc ggcgcggtgc tgccgggcaa gcgctaccac cacaaggaca
      361 cggcgcagat cttcgcggca tgccacgtgc cctacgtctt cacggcgagc gaaggctacc
      421 ccgaggacct catgcgcaag gtggccaagg cgcagtggta cgcgaagaac gaagggctcg
      481 tgtacggcaa gatcctgtcc tactgcccgc tcaactggaa gacggccgac gatgccgcgc
      541 agcccgtcct gcaggccgcg atcgactgct gcttcttccc gctctacgag atcgagcacg
      601 gccataccac gctcacctac gatcccgacg gttcagaccg ccgccacccc gtgtcggact
      661 ggctcaagct catgggcaag acccgccacc tgctcaagcc cgccaacgcg gccatcgtgg
      721 cggacatcca ggccgagacc gaccggcgct gggcacgcat caaggcgatg cacgagcatc
      781 cgctgctcta ggccccggga aaacgacatg gcgaaaatca tcgagacacg gcggctcacc
      841 cccgtcacca agtacttccg catcgaggct ccgctcatcg cctcctcggc aaggcccggg
      901 cagttcgtga tgctgcgcgt tcgcgacggc ggggagcgca ttcccatcac catcgccgac
      961 tacgaccggc aggccggcac gatcaccatc gtcgtgcagg aggtgggagc gaccacgaaa
     1021 cgggtgtgcg cgctcgaggc gggcgacgag atcctcgacg tggccggccc ccttggcggg
     1081 cacatcgaca tcgcgcctgg cggccacgtc tgcggcatcg gcggcggctt cggctcggcg
     1141 gcgctgctct gcctgatgcg cgagttcacc gcccgcgggg acacgaccac cgccatcctc
     1201 ggcgcgcgca acaaggagct gctgatcctg gccgacgagc tgcggccgct gtgcacccac
     1261 ctcgagatct gcaccgacga cggctcggcc gggttcaagg gtttcgtcac gcagcggctg
     1321 aagcagttga tcgacggcgg cggcgagcgc cgccccgaca gcgtcgtggc gatcggcccg
     1381 atggcgatga tgcgcgccgt cgcggagacg acgcgcgcgg cgaaggtgaa gacgctggtg
     1441 tcgatggacc cgctgatggt cgacgggacg gggatgtgcg gcggctgccg cgtcaccgtc
     1501 gccggcaagg cgcagttcgc ctgcgtggac ggcccgtgct tcgacgcgca cgaggtcgat
     1561 ttcgacgtgg cgatgcgccg caacaaggcc tacgtgcagg aagagaagca ggccctggat
     1621 cgcgtcgcgt gcatggaacg cagggaggca ccgtaatgcc catcaagcga tcggacaaga
     1681 ccccgatgcc ggtgcgcgag gcgcagatgc gcgcgcgcga cttcatcgag gtgaacgagg
     1741 ggtacacgaa gtcgcatgcc agcttcgagg cggagcgctg cctgcgctgc caggacccgg
     1801 tgtgcgtcgg cggctgcccg gtgaacgtgc cgatcccgga attcatccac gccgtggcga
     1861 tgggcgacat ggccggggcg gcgaggatcc tgcgctcggc caacccgctg ccggccatct
     1921 gcgggcgcgt ctgcccgcag gaatcgcaat gcgaggccga ctgcagcatg acgacgcgct
     1981 tcacgccggt ggccatcggc cacctcgagc gcttcgtggc cgactgggag cgcacgcagc
     2041 ccccctcggt ggaagcgaag atcacgcgca aggagaagat cgccgtcatc ggcgcgggcc
     2101 cgtcgggtct ggtgtgcgcg ggcgcgctgg cacgcctcgg ctacccggtc accatctacg
     2161 aggcgctgca cgccgcaggc ggcgtgctcc gctacggcat accggagttc cggctgccca
     2221 agcaggtgct cgactgggaa atcgggctgc tcaagtcgat gggcgtggaa atcgtctgca
     2281 acgtcatcat cggcaagacg ctcacgctcg acgagctgtt cggcaagatg ggctttgcgg
     2341 ccgtgttcat cgggaccggc gcggggctgc cgcagttcct tggcattccc ggcgagaacc
     2401 tgaatggcgt catgtcggcc aatgaattcc tcacccgcat caacctgatg cgcggctacg
     2461 agtttcccga atcggacacg ccggtgcgcg tcggcaagcg cgtcgcggtg atcggggcgg
     2521 gcaacacggc gatggacgcg gtgcgcacct cgctgcgcat gggcgccgag gaagcgatga
     2581 tcgtctaccg gcgcagcgac aaggaaatga gcgcgcgcgt cgaggaatac caccacgcgc
     2641 aggaagaggg cgtgcagttc cactggctca ccaatcccct cgaggtggtg ggcaatccgg
     2701 agggctgggc gacgggcctg aagtgccaga agatgaagct gggcgagccg gacgcctccg
     2761 gccgggcacg cccggtgccg attccggggt cggagttcgt gatcccggtg gacaacgtga
     2821 tcctcgccat cggcacgacc ccgaacccgc tcctcacccg cacgacgtcg gggctgctca
     2881 cgaatccgaa gggatgcctg atcgcggacg agagcaccgg gctcacgtcg aagccgctgg
     2941 tgttcgccgg cggagacgcc gttaccggcg cggcgacggt gatccttgca gccggcgcgg
     3001 gcaagcgcgc cgggcaggcg atccacgaag cgctggtcga gcggagccgg gtgccggcct
     3061 aggcgcgcgt attgcggcgt acgcgccgct cgccacccag cgcaaggccg ggcccgccgc
     3121 cggccgccag gagcttgtcc cattcgacgg cgtccgcgcg cgcgtactcc agggcctgcg
     3181 tcacccactg cacgtgctcg gactcctcgc gggccatttc cgtcgcaagg gcatggacct
     3241 cgccatcggc cgtcgttccg gcgacccact cgaagaaccg gcgcgcgttc cgctcggcct
     3301 tgagggcgat ctcgagaagc tggcgcggcg tggccacgcg atagacaagt tcgtgcgcgg
     3361 cgctctcggg cggccccgat tcgagccagc gatagcgctc gggcgtgagc gcgggcagcg
     3421 tgcgcccttt cgtgcgctcg aggagcagct cgtagtgctc ctgctcgaag tgcgcgatgt
     3481 tgccgcacag gccggccagc acttcctcgc cgtgatcgtt gaaatgcgcc tggaactcgc
     3541 gatagctcct ggcagcctcc tgctcgatgg cgagcgcgtg ggcaaggaat tcctcgatcg
     3601 atccgatggg ctcgagcatg gtgcgacgcc tcctcacgct tcacgatgcg cctaggttat
     3661 agggcgcgat tgacttgggt caacggggct tcgcgattgg gtctagaacc gggcattgac
     3721 cgcgatggcg aaggactcgt cgacactctt cgcgccgatg cgcgacacga gcagctccac
     3781 gctccagcgc ttgtcctgcg tttcccagta cgcctgcagg gttccgtccc actcgggtcg
     3841 caggcggttg gcaggggtgt tggcttcgtc gcggaaacgc tcgccgcgat agatggcctt
     3901 cgcgccgatc gagaagcgat ggtcggaata gaacgtcgca cccagcgccc cgcccttctc
     3961 cgggagatag gcgaaacgct tgccggggta ggcgagcgta ttctcgctgt cggaccacgc
     4021 tccctccgca taaagggaga gatggcggtt gacgatgtag ttcactgccg cgcccgcctc
     4081 gcgcaacgtg ccccgggaga ggtccggaaa cccctcgagc tggtcgagtg tcgcgaggtt
     4141 gttgaacgac cggttgcgca gccgctccag gttcgacgaa tcggggcgat tgttgagcac
     4201 gccgatcagc gcggagtaga ggttgtcgat ctcctggcgc tgcgcatggg ccgtgacgag
     4261 caagccgcct ccggcctccc actcgacctg cgcgcgggcc tgctcgagcc tgccgcccgg
     4321 cagcacgaag cgatcgtcga ccatgatgcc ggccgtggcc gtggccgaca gcgaaccgat
     4381 ggacgccggg cgcagccact gctgccatgc aaggcgcaag gtcgtgccgg gcaatgcacg
     4441 ccactcgagc ccggcgcggc cattcacttc gtcgcgcgcg tggttgtcgg cgagcaggac
     4501 gagctggtcg gcaaaatcgc ggcgcaccac gatgccgttg gtcttgtcgt actcggtccg
     4561 gtcgacctgc acctcgatcg tcgccatgcc gagctgcgcg tggccggcca gcgccaccac
     4621 gcgcgagtcg tcgcggatgt cctggcgcac ggattcgatc aagcctgtcg cagccggcgc
     4681 gtgcgcgaaa gcgtcgcgct cgaggacatc gaccgatttc cagcgcgcgg actcgtaacc
     4741 cagggagagc acgtgcccgc cctcgaagcg cacgagcgcc cgcgcctggg cgtcgcggcg
     4801 ctccggcctg gagatgaaat ccgagccctg cacgacgagc gcctgcgccg ttccggctgc
     4861 gggcgcgacg tttgcgcgaa tccggctctc ctcgtcaccg ctgccgacct tcacccagaa
     4921 gaggcggtcg gcggattggc gccagcccat tcccgcgtcc acgcggcgcg ccgtcccgtc
     4981 gatgatcgag aagtttcggc gcgggtcgtc ggcaccggcg cgcacatcgg gaacgagccg
     5041 gttgccgtag gcgaagaagc cgatgtcgtg gcgcaacttc gcgccgatcc cgaaggtggt
     5101 cgaggtcgca cggtcctcct gcgtgccatc ggtgcgccat tgcctcagcc gtgcgccttc
     5161 ggcaaaccac gccaggcgcc cgtccgcggc gagtccattg gcggtcaggg cgggctcgac
     5221 gaggcgcacg tcgtcgccgg tggcgccgcg ccatgcaagc gtggcgtacg cgcccggctt
     5281 cgggaccagc gcctggaagc ggttcgagcc gccaagggcg gtcgggtcgg caaggaatcc
     5341 ctggaagagc tcggagttgc gtgcgtaacg gcttgggtag cggtcggcga ggaagaaatg
     5401 gctgccggcc cagaaggggt cgtaggattc ctgcgcattg cgcagcgccc aagcttccag
     5461 cccgaactgc gcaagggccg cgccgagatt ggccacgccg cgcgtgct
//
LOCUS       DASUEV010000110         6494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_144336, whole genome shotgun sequence.
ACCESSION   DASUEV010000110 DASUEV010000000
VERSION     DASUEV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144336"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFV55_03600"
     CDS             <1..482
                     /locus_tag="VFV55_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4943411.1"
                     /translation="ITKLSEAGLADRRASHVGFVFQFYNLMPVLNAFENVELPLLLTS
                     LSRRERRERVAMALELVDLSDRRDHYPNELSGGQQQRVAIARAFISDPTIIVADEPTG
                     DLDRRSAAEILGLLRRLNAEMGKTIIMVTHDWHAVESARHVVHLEKGELVDAPATHA"
     gene            499..2025
                     /locus_tag="VFV55_03605"
     CDS             499..2025
                     /locus_tag="VFV55_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943412.1"
                     /translation="MRQLLAASLLGPAVALAAPGDFDRSFGQDGVATLGPGPVADQAD
                     SAVFVDSDGQGRLLVIGSVLQPTNGVDVSVRLFARFLPEGIPDATLSGTGYIHTAPDP
                     STNRVGLRAYAVANGRTLLVEQHRFICQPSRQPCGLPVDVPHFFAQRIEPGGEIDSSY
                     GVMATVSMDVVQEDVAVSPDGSLTVAGYQYPPAIPGAFPDPRFDVRGVDPAGQLFLPW
                     FGARPSFDCGGDARPAARSAKMARQPDGRFLFVQQVSDAAGNNRLCVSRLNPDATLDT
                     SFGSAGRLRVDDPRFAGGPATILALLARSAGGAILFLKPFGLGGSPLYMAWLTADGAL
                     DASRGVQGIVGPFAGPIDDFQTIALQPDDGILLAGFPLPRLAQAKVIPPIPDFGQPRI
                     VRLDSFGMLDPGFGPTGEGYAPLVSSGRGIWPRHLHVGSDGAIFVAGSIGVGNLALAA
                     AQSRFAVAKLEGGARHESRGGIWGSGCGSTRNAPVDPTLPALTLLAAALLLARSRKSR
                     "
     gene            2025..3188
                     /locus_tag="VFV55_03610"
     CDS             2025..3188
                     /locus_tag="VFV55_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4943413.1"
                     /translation="MYYLKLVFKNIFRHKLRSLLTLFGLVVAILAFGLLQTVVNAWYA
                     GSDMASAQRLISRNAISLVFPLPIYYRDRIKAVDGVTAVGVSNWFGGIYKDMTPGNFF
                     AQFAVDHETFFDLYPEFKVSPEELLAFKKDRRGALVGRLLADQHGLKVGDVVPLKGTI
                     YPGNWEFVVRGIYEPRDETTITRQLYFHWELLNEEIKRKYPRRAEQVGVFVVGIADGS
                     RAAEISQSIDREFRNSLAETLTETEKAFQLSFIAQTETIIVAVRIVSFVVIVIIFAVV
                     ANTMAMTARERLAEYATLKALGFGPGFVAALIVGESLMITALGGALGILATFPAAAAF
                     KAVLGSFFPVFKVAPETVAMQAASALFVGLLAGILPSIRAARVKIVDGLRYIG"
     gene            3185..4351
                     /locus_tag="VFV55_03615"
     CDS             3185..4351
                     /locus_tag="VFV55_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4943414.1"
                     /translation="MKIPLSYIFRNLWTRKLTTLLTAGGMALVVFVFAAVLMLDAGLK
                     KTLVGTGSYENAVLLRPAAQTEIQSAVYRDQASLVETLPEVARGADGTPLVSKETLVL
                     IAIPKRGSDKPANLIVRGMPAMGIRLRPQVRVIDGRMFRPGSSEIVVGSAITDGFDGT
                     GIGEKLRFAGREWTIVGTFDGGKSGFDSEIWGDVDQMLQAFRRSTYSSVLARLGSVGQ
                     FDAFQARIAADQRLILDVKQEPLYYQDQSKSLSNFISYLGVALSVIFSIGAMIGAMIT
                     MYASVANRTGEIGTLRALGFRRTSILAAFLTESLLLALVGGVAGLVLASFLQAFTITT
                     MNWQSFSQLAFGFHLTPSIVVSTLAFSLVMGLVGGFLPSVRAARLEIVDSLRAA"
     gene            4497..5600
                     /locus_tag="VFV55_03620"
     CDS             4497..5600
                     /locus_tag="VFV55_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEX4943415.1"
                     /translation="MALNRLLATYRVASSAGEIDARAKALAAEQSVEMPLEAIGDPRV
                     LQEIAATVESIRPAGSAFEVTLGLAADTTGNEASQLANMLFGNCSLQPEVELLDVAFP
                     PGYESAFPGPAFGIAGLRQRVHAPSRALTCTALKPQGSTVAHLAKLAGIFARAGIDVI
                     KDDHGLANQAYSPFAERVPAVQRAIDDANRETGGHTLYAPTFSGGPGALAEQARIAVN
                     CGVGIALVAPMLVGLPVFVEMRREHGLALLAHPAFAGASRVSPPLLLGKLFRLFGADA
                     TIFPNHGGRFSYSRETCLGIAAAARDPWNGIKPALPVPAGGMTVERVGEMIAGYGTDT
                     MLLIGGGLLTAGDALLERSRDFVAKVASHGRCA"
     gene            5597..6028
                     /locus_tag="VFV55_03625"
     CDS             5597..6028
                     /locus_tag="VFV55_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX4943416.1"
                     /translation="MTDKLVRRHTGAFHWEGVEVLEYKHEGTAPFRDVTRQVLFDDPS
                     LTTQLRYFEVAPGGWTTLERHEHVHNVMVIRGRGRCRIGETTCDLAQNDLVSVPPMTW
                     HQFHAADDEHLGFLCLVNRMRDRPQLPPGQEGRQSEPTPPR"
     gene            6154..6447
                     /locus_tag="VFV55_03630"
     CDS             6154..6447
                     /locus_tag="VFV55_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX4943417.1"
                     /translation="MATYIALANFTDQGIRNVQDTTKRAEAVREMAKKCGVTMKDIYW
                     TLGSYDMVVTFDAPDDASMTAVGLSIAKAGNVRTQTMRALSRDDMNGILAKML"
BASE COUNT         1015 a         2241 c         2145 g         1093 t
ORIGIN      
        1 acatcacgaa gctctccgaa gccgggctcg cggataggag ggcctcgcac gtcggcttcg
       61 tcttccagtt ctacaacctc atgccggtgc tgaacgcgtt cgagaacgtg gagctgccgc
      121 tgctcctcac ctcgctttcg cgccgcgagc ggcgtgagcg cgtcgcgatg gcgctggaac
      181 tggtggacct ttccgaccgc agggatcact accccaatga gctctcgggc ggccagcagc
      241 agcgcgtggc catcgcgcgc gccttcatct ccgacccgac gatcatcgtc gccgacgagc
      301 ccaccggcga tctcgaccgc aggagcgccg ccgagatcct gggcctgctg cgccggctga
      361 acgcggagat gggcaagacc atcatcatgg tcacccacga ctggcacgcg gtggaaagcg
      421 cgcggcacgt ggtgcacctg gagaaggggg aactggtcga tgctcccgcg acccacgcgt
      481 gacaggaggg caggttcaat gaggcagctc cttgccgcat cgcttcttgg tccggcagtc
      541 gcgctcgcgg cgccggggga tttcgaccgg tccttcggcc aggacggtgt ggcgacgctc
      601 ggcccggggc ccgtcgcgga ccaggcggac agcgccgtct tcgtggattc cgacgggcaa
      661 ggacgtttgc tcgtgatcgg cagcgtcctc cagcccacca atggggtcga cgtatccgtg
      721 cgactcttcg cgcgcttcct cccggaaggg attcccgatg cgactctctc gggcacggga
      781 tacatccaca ccgcgcccga tccgtccacc aaccgcgtcg ggctgcgggc atacgccgtc
      841 gcgaacggca ggactctgct cgtcgagcag caccgcttca tttgccagcc ctcgcggcag
      901 ccttgcggac tgcccgtcga cgtgccccat ttcttcgcgc agcgcatcga gcccggcggc
      961 gagatcgact cctcgtatgg cgtcatggcc acggtgtcga tggacgtcgt gcaggaggac
     1021 gtcgccgttt cacccgacgg ttcgctgacc gtcgcgggct accagtaccc gcccgccatt
     1081 cccggcgcgt tcccggaccc gcgtttcgac gtacgcggcg tcgatcccgc gggacagctg
     1141 ttcctgccct ggttcggtgc ccgcccttcc ttcgattgcg ggggcgacgc ccgcccggcg
     1201 gcgcgcagcg cgaagatggc gcggcaaccg gatggccggt ttctcttcgt gcaacaggtc
     1261 tcggatgcgg cgggaaacaa ccgcctgtgt gtctcccgcc tcaaccccga cgccaccctc
     1321 gatacgagct tcggaagcgc aggacggctt cgagtcgatg atccgcgctt cgccggaggt
     1381 ccggccacga tcctcgcgtt gcttgcgcgc agcgcgggcg gcgcgatcct gttcctgaag
     1441 cccttcggcc tcggcggcag cccgctgtac atggcctggc tgacggccga cggcgcgctc
     1501 gatgcgtcgc gtggcgtgca gggcatcgtc gggcccttcg ccggcccgat agacgacttc
     1561 cagacgatcg ccttgcagcc cgacgacggg atcctgctcg ccggtttccc cttgccgcgg
     1621 ctcgcgcagg caaaggtgat tcccccgatt ccggatttcg gccagccgcg catcgtccgc
     1681 ctggattcct tcggcatgct cgaccccggg ttcggcccca cgggcgaggg atacgcgccg
     1741 ctcgtgtcgt ccggccgggg aatatggccc cgccaccttc acgtcggcag cgacggcgcc
     1801 atcttcgtcg ccggcagcat cggcgtgggg aatctggcgc tcgcggccgc gcaatcgcgc
     1861 ttcgccgtcg cgaagctcga agggggcgcc cggcacgaat cccggggagg catctgggga
     1921 tcgggctgcg gcagcacccg gaacgcgccc gtcgatccca cgctgcccgc gttgacgctg
     1981 ctcgcggcgg cactgctgct tgcgcgatcg cgaaaatccc gctgatgtac tacctgaagc
     2041 tggtcttcaa gaacatcttc cgccacaagc tgaggagcct gctcacgctc ttcggcctgg
     2101 tggtggcgat cctcgccttc ggcctgctgc agacggtggt gaacgcgtgg tacgcgggct
     2161 cggacatggc ctccgcgcaa cggctgatct cgcgcaacgc gatctcgctc gtctttccgc
     2221 tgccgattta ctaccgcgac cggatcaagg cggtggacgg ggtgacggcg gtgggcgtct
     2281 ccaactggtt cggcggcatc tacaaggaca tgacgccggg caatttcttc gcccagttcg
     2341 cggtggacca cgagaccttc ttcgacctct accccgagtt caaggtgagc ccggaggagc
     2401 tcctcgcgtt caagaaggac cggcgcggcg ccctggtggg gcggctgctc gcggaccagc
     2461 atggcttgaa ggtgggcgac gtggtgccgc tgaagggcac gatctacccg ggcaactggg
     2521 agttcgtggt gcgcggcatc tacgagccgc gcgacgagac caccatcacg cgccagctct
     2581 acttccactg ggagctcctc aacgaggaga tcaagcgcaa gtacccgcgg cgcgcggagc
     2641 aggttggcgt cttcgtggtc ggcatcgccg acggctcgcg cgccgccgag atcagccagt
     2701 cgatcgaccg cgagttccgc aattcgctgg cagagacgct caccgagacc gagaaggcct
     2761 tccagctctc cttcatcgcg cagaccgaga cgatcatcgt ggcggtgcgc atcgtctcct
     2821 tcgtggtgat cgtgatcatc ttcgccgtgg ttgccaacac catggcgatg accgcgcggg
     2881 agaggctcgc ggaatacgcg acgctcaagg ccctgggctt cgggccgggc ttcgtcgccg
     2941 cgctcatcgt cggcgagtcg ctcatgatca cggcgctggg cggggcgctc gggatcctcg
     3001 ccacgttccc cgcggccgcc gcattcaagg cggtgctcgg ctcgttcttc ccggtgttca
     3061 aggtggcgcc ggagacggtg gcgatgcagg ccgcctcggc gcttttcgtg ggactgctgg
     3121 cgggcatcct gccgtcgatc cgggccgccc gggtgaaaat cgtggacggc ctgcgctaca
     3181 tcggatgaag atcccgctct cctacatctt ccgcaacctg tggacgagga agctcacgac
     3241 gctcctcacc gcgggcggaa tggcgctcgt ggtgttcgtg ttcgccgccg tgctgatgct
     3301 cgacgcgggc ctgaagaaga cgctcgtggg caccggcagc tacgagaacg cggtgctgct
     3361 gcggccggcg gcgcagacgg aaatccagag cgccgtgtac cgcgaccagg cgtcgcttgt
     3421 cgagacgctg cccgaggtcg cgcgcggtgc cgacgggacg ccgctcgtct ccaaggagac
     3481 cctcgtcctc atcgcgatcc cgaagcgcgg cagcgacaag cccgctaacc tcatcgtgcg
     3541 cggaatgccg gccatgggca tccgcctgcg gccgcaggtc cgggtcatcg acggccgcat
     3601 gttccgcccg ggttcgtcgg agatcgtggt gggcagcgcg atcaccgacg gcttcgacgg
     3661 cacgggcatc ggcgagaagc tgcgcttcgc ggggcgcgag tggacgatcg tgggcacctt
     3721 cgacggggga aagagcgggt tcgattccga gatctgggga gacgtggacc agatgctgca
     3781 ggccttccgc cgctcgacct attcctcggt gctcgcccgc ctgggaagcg tggggcagtt
     3841 cgacgccttc caggcgcgca tcgccgccga ccagcgcctc atcctggatg taaagcagga
     3901 accgctttac taccaggacc agtccaagtc gctctccaac ttcatcagct acctgggcgt
     3961 cgcgctctcc gtcatcttct cgatcggcgc catgatcggc gccatgatca ccatgtacgc
     4021 gagcgtggcc aaccgcacgg gcgagatcgg gacgctgcgc gcgctgggct tccggcgcac
     4081 gagcatcctc gcggcattcc tcaccgaatc gctcctcctc gccctcgtgg gcggcgtggc
     4141 gggcctggtg ctcgcctcgt tcctgcaggc gttcaccatc accaccatga actggcagag
     4201 cttcagccag ctcgcgttcg gcttccacct caccccttcc atcgtggtct cgacactcgc
     4261 cttctcgctc gtcatgggac tggtgggcgg gttccttccc agcgttcggg cggcgcggct
     4321 ggagatcgtg gattcgctgc gggcggcgta aggggcccgg attcgccgag agttgcctgg
     4381 acgcaacgcg atgacgtcat ggcgtcatgt tgctacagtg tcgccatagt caagtcgctg
     4441 cgaaagcgcg gaaagatcta ggcttgtcat caaggcttcg gccacgaggt gtatcgatgg
     4501 ctttgaaccg gctcctcgcc acctaccgcg tcgcctcgtc cgcgggagaa atagacgcgc
     4561 gtgccaaggc gctggccgcc gaacagagcg tggagatgcc cctcgaggcg atcggcgacc
     4621 cgcgcgtgct gcaggagatc gccgcgaccg tcgaatcgat ccgtcccgcc ggcagcgcct
     4681 tcgaggtgac gctgggtctc gccgccgaca ccacgggcaa cgaggcatcg caactggcca
     4741 acatgctctt cggcaactgc tcgctgcagc cggaggtgga actgctcgat gtcgcatttc
     4801 ccccgggata tgaaagcgcc tttcccgggc cggccttcgg catcgccggc ctgcgccagc
     4861 gcgtacacgc gccatcgcgt gccctcacct gcaccgccct caagccccag ggctcgaccg
     4921 tggcgcacct ggcgaagctc gccggcatct tcgcgcgcgc gggcatcgac gtcatcaagg
     4981 acgaccacgg gctcgccaac caggcgtact cgcccttcgc cgagcgcgtg ccggcggtgc
     5041 agcgcgccat cgacgatgcg aaccgcgaaa ccggcgggca tacgctctat gcgccgacgt
     5101 tttccggcgg gcccggggcg ctggccgaac aggcacgcat tgccgtgaat tgcggcgtgg
     5161 gcatcgcgct cgtggccccg atgctcgtgg gcctgccggt cttcgtcgag atgcgccgcg
     5221 agcacggcct ggccctcctc gcccaccccg ctttcgccgg cgcctcgcgc gtgtctcccc
     5281 cgctgctgct cggcaagctc ttccgcctct tcggggcaga tgcgaccatc ttcccgaacc
     5341 atggcggccg cttcagctac agccgcgaga cctgcctcgg catcgccgcc gcggcgcgcg
     5401 atccgtggaa cggaatcaag ccggcgctgc cggtgcccgc gggcgggatg acggtcgagc
     5461 gcgtgggcga gatgatcgcc ggctacggaa ccgataccat gctgctcatc gggggcgggc
     5521 tgctcaccgc cggcgacgcg ctcctggagc gcagccgcga tttcgtcgcg aaagtcgctt
     5581 cgcacgggag atgcgcatga ccgacaagct cgtccgccgc cacaccggcg cctttcactg
     5641 ggaaggcgtc gaggtgctcg agtacaagca cgagggcacg gcgcccttca gggacgtgac
     5701 gcgacaagtg ctcttcgacg acccctcgct caccacgcag cttcgctact tcgaagtcgc
     5761 ccccggtggc tggaccacgc tcgagcggca cgagcacgtg cacaacgtga tggtgatccg
     5821 cggcaggggc cgctgccgaa tcggcgagac gacgtgcgac ctggcgcaga acgacctggt
     5881 gagcgtgccg cccatgacct ggcaccagtt ccacgccgcg gacgatgagc accttgggtt
     5941 cctttgcctg gtcaatcgca tgcgcgaccg gccgcagttg ccccccggcc aggagggtcg
     6001 gcaatccgag ccgactccgc ccaggtaacg tcgatcggac ggccgcgagg cggatcgcat
     6061 acgtccacgc agagcctcag gcgggcacca tggaggccgg tgccaccgcc agcaaacgtt
     6121 cccttggcct ccctcttccg aaggagatca ttcatggcga cctatattgc gctggcgaac
     6181 ttcaccgacc agggaatccg gaatgtccag gacaccacca agcgggcgga agccgtaagg
     6241 gaaatggcga agaaatgcgg cgttacgatg aaggatatct actggacgtt gggcagctac
     6301 gacatggtcg tcacgttcga cgcgccggac gatgcctcca tgaccgccgt cggactgagc
     6361 atcgcaaagg cgggaaacgt gcgcacgcag acgatgcgag ccctgtcccg ggacgacatg
     6421 aacgggattc tcgccaagat gctgtagccg acggtgtcag aagggtaaaa ggggtcaggt
     6481 tactttgcac aaaa
//
LOCUS       DASUEV010000111         6911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1161661, whole genome shotgun sequence.
ACCESSION   DASUEV010000111 DASUEV010000000
VERSION     DASUEV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161661"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..770
                     /gene="ampD"
                     /locus_tag="VFV55_03635"
     CDS             252..770
                     /gene="ampD"
                     /locus_tag="VFV55_03635"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX4943418.1"
                     /translation="MCSGARWVPSPNQDLRPDDAAVSLLVIHNISLPPGEFGGEWITD
                     LFLDRLHPGAHPYFAGLAGLRVSAHFLIRREGETIQYVPCELRAWHAGDSSWNGRERC
                     NDFSVGIELEGTDYLPFTDAQYESLGELTLALFARYGRLDLAGHSDIAPGRKTDPGPH
                     FDWARYRAAINA"
     gene            836..2032
                     /locus_tag="VFV55_03640"
     CDS             836..2032
                     /locus_tag="VFV55_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4943419.1"
                     /translation="MQAFMRDHVFPAEDGYRDELVGGADWRRWKQPAIMESLKERARR
                     EGLWNLFLPHAGLGAGLSNADYAPLAEITGHSLIAPEVFNCNAPDTGNMEVLALYGSA
                     EQKERWLDPLLAGRIRSAFCMTEPEVASSDATNMRATAVVEGNEVVLNGRKWWSSGLG
                     HPDCRVAIFMGLSEPNADKHHQHSMVLVPLDLPGVRIERMVPCFGVYDEPFGHGEVSF
                     TNVRVPKENVVAGIGRGFEIAQGRLGPGRIHHCMRVIGAAERALELLCARAESRVAFG
                     KPLALLGGNADVIANARMAIDQARLLTLRAAWMMDSVGVKDAMSEISQIKVVAPGMAQ
                     QVIDAAIQVHGGAGVSDDFPLTALMGYARILRIADGPDEVHRALIAKLELRRQRAKRE
                     ADGIAP"
     gene            2029..3111
                     /locus_tag="VFV55_03645"
     CDS             2029..3111
                     /locus_tag="VFV55_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX4943420.1"
                     /translation="MTDASSLVDRAGGVRAGEELDAGKVDAFLKSRIAGLAGTPEIGQ
                     FSRGASNLTYLVRYPGRDLVLRRPPFGHKAKSAHDMLREARVMTALKPVYPVVPAVLA
                     IGDDPSVMGCEFFVMERIAGIIPRQDLPAGLGFGEAQTRRLCLNVVDKLVELHAIDPQ
                     AAGLADLGKGEGYVRRQVEGWSRRFRDARTDDVSDFESVMAWLAVRRPEREVAIRVIH
                     GDYRFDNVVLDPADPFRVIGVLDWEMATLGDPLMDLGNSLAYWVQADDDEYFRMTRRQ
                     PTNAPGMLTRAQVIEHYAARTGLPVANFDFYAVFGLFRVAVIVQQIWRRYREGATKDP
                     QFAAFGPLVTYLDGRCRRLVADSHGV"
     gene            3152..3994
                     /locus_tag="VFV55_03650"
     CDS             3152..3994
                     /locus_tag="VFV55_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4943421.1"
                     /translation="MKEFAGKVAVITGAAEGIGRAVAEEAAARGMKLLLADIDAARLD
                     ATTAELRAKGADADGLRTDVSKASQVDDLADLAFGRFGRVHLLVANAGVACAKPVWDT
                     SPEDWEWVMGVNFYGVTHVLRAFLPRMMAGGEEGHVVATASMAGLLSLPGMAAYNASK
                     HAVVTVMEGLHHDLTLRSAKIGACVLCPSWVKTRIALSERNRTSGEVTRPEDLDPVTA
                     RLAAAVQRAVDGGIEPGEAARAVFAAVSDGRFYVLTHGDSKSGVKARLDAILHDRVPP
                     LVRL"
     gene            4348..>6911
                     /locus_tag="VFV55_03655"
     CDS             4348..>6911
                     /locus_tag="VFV55_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX4943422.1"
                     /translation="MRMVSDVKPTTPLPDPVQSEEGVNLPERYADYKVIRRNGAVVGF
                     EPDKIAVAVTKAFLAVAGGTAAASAGVREKVHGVTQAVVNALVRRHPAGGTYHIEDIQ
                     DQVELALMRAGEHQVARSYVLYREERARERAARAQEAAQARQPKLHVTVNGGKVPLDL
                     ASLEALIVSACQDLGDAVEPGTILDATLRDLYDGVPIEEVHKCAILAARMLIEQDPAY
                     TYVSARLLLHSIRREVLGEEVAHEQMASRYAEYFPTFIQQGIRAQLLDERLGTFDLVR
                     LGEAIRSSRDLKFDYLGLQTLYDRDFLHIHDHHIELPQAFFMRVAMGLSINEVHREER
                     AIEFYNVLSTFDFMSSTPTLFNSGTQRSQLSSCYLTTVADNLDGIYEAIKENALLSKF
                     AGGLGNDWTPVRALGSYIKGTNGKSQGVVPFLKVVSDTAVAVNQCFAPETGVYTADGL
                     KPIKDVKPGDLVLGKSGQYREVERRFAYNQTDAMVAVKVKHAIDPLLVTTGHPFFAIQ
                     CVPMEQANSRTLAWLAKGKVKAEWVEAGQLQAGDYVAQVVPTEVVNVPGLSEDDARFY
                     GILLGDGHLSKDCLEWGVSGNPLKDEHLAFVRAYLAERGIHYWEHQRGENYGQVHWAS
                     GRGVVRNGTTGRIVGAGAPTMPFGHEDLYDEAGRKHVARRFAHLPKAHTLAMIRGLLE
                     TDGGVSRGKEIYFTNTSKPLVDGLRYQLLRMGVPTAGQYRERENGHTATRSDGSTTTF
                     VGTTKAYDLRIPAVPVIAGLVGCKPIVKRNWLEIEGKLFSRITEIQAITPKPFVYDLK
                     VETDESYMTEAGLAHNGGKRKGAVCCYLETWHLDIEEFLELRKNTGDDRRRTHDMNT"
BASE COUNT         1174 a         2362 c         2289 g         1086 t
ORIGIN      
        1 acttcggcaa cacctcgaca taggttcgaa ccgtgacgcc cgacgcatat tgacctggcc
       61 caaaatcaaa aaaaacccaa cccacctgac cccttgtatg ccatgggcac attccctcgc
      121 acatcggcct gaccgcaatt ccggttggcg actatcggca cattccctcg cacatcggcc
      181 tgaccgcaat tccacgcatg gacatcgata acgacatcac tccaccgcgc ttcctcgtgg
      241 acatcgcagg catgtgctcg ggcgcacgct gggtgccgtc gcccaatcag gacctgcggc
      301 ccgacgatgc cgcggtctcg ctcctcgtga tccacaacat ctcgctgccg ccgggcgagt
      361 tcggcgggga gtggatcacc gatctcttcc tcgaccgcct gcacccgggg gcgcacccgt
      421 acttcgcggg gctggcgggg ttgcgcgtgt ccgcgcactt cctcatccgc cgcgaaggtg
      481 agacgatcca gtacgtgcct tgcgagctgc gcgcctggca cgcgggcgac tcgagctgga
      541 acgggcgcga gcgctgcaac gacttttccg tcggcatcga gctcgagggc acggactacc
      601 tgcctttcac ggacgcgcag tacgagtcgc tgggcgaact cacgctggcg ctcttcgcac
      661 gctacggaag gctcgacctt gccgggcatt ccgacatcgc gccggggcgc aagaccgacc
      721 ccgggccgca tttcgactgg gcgcgctatc gcgccgccat caacgcctga acacgggccg
      781 gacaccgacc atggacttcg caccctcttc caaagccgca gaattcatcg cccgcatgca
      841 ggccttcatg cgcgaccacg tcttccccgc cgaggacggc taccgcgacg aactggtggg
      901 cggggccgac tggcgccggt ggaaacagcc ggcaatcatg gaatcgctca aggagcgcgc
      961 gcgccgcgag ggcctgtgga acctgttcct gccgcacgcc ggcctggggg cgggcctgtc
     1021 gaacgcggat tacgcgccgc tcgcggagat aaccggtcac tcgctcatcg cgccggaagt
     1081 gttcaactgc aacgccccgg acaccggcaa catggaggtg ctcgcgctct acgggtcggc
     1141 ggagcagaag gagcggtggc tcgaccccct gctggcgggc aggatccgct ccgccttctg
     1201 catgaccgag ccggaggtgg cctccagcga cgcgaccaac atgcgcgcca ccgccgttgt
     1261 cgagggcaac gaggtagtgc tcaacggccg caagtggtgg agctcggggc tcggccaccc
     1321 ggactgccgc gtggccatct tcatggggct ctccgagccg aatgccgaca agcaccacca
     1381 gcattcgatg gttctggttc ccctcgattt gcccggcgtc aggatcgagc gcatggtgcc
     1441 ctgcttcggc gtgtacgacg agcctttcgg ccacggcgag gtctcgttca cgaacgtgcg
     1501 cgtgccgaag gagaacgtgg tcgccggcat cgggaggggg ttcgagatcg cgcagggccg
     1561 cctcggcccc gggcgaatcc accactgcat gcgcgtgatc ggcgcggcgg agcgcgccct
     1621 cgagctgctc tgcgcgcggg ccgagagccg cgtggcattc ggcaagcccc tggccctgct
     1681 cggcggcaat gccgatgtca tcgccaacgc gcgcatggcc atcgaccagg cgcggctcct
     1741 cacgctgcgg gccgcgtgga tgatggactc cgtcggcgtg aaggacgcca tgagcgagat
     1801 ctcgcagatc aaggtggtgg cgcccggcat ggcgcagcag gtgatcgatg cggccatcca
     1861 ggtccacggc ggcgccggcg tctcggacga tttcccgctc accgcgctca tgggatacgc
     1921 gcggatcctg cgcatcgccg acggccccga cgaggtgcat cgcgcgctca tcgcgaagct
     1981 ggaactgcgc aggcagcgcg cgaagcgcga ggccgacggc atcgccccat gaccgatgcg
     2041 tcctccctcg tggaccgcgc aggcggcgtc cgcgcggggg aggagctcga tgcggggaag
     2101 gtggatgcgt tcctcaagtc gcgcatcgcg ggcctggcgg gcacgcccga aatcggccag
     2161 ttctcgcgcg gggcgtccaa cctgacctac ctggtgcgct acccgggacg ggacctcgtg
     2221 ctgcgccgcc cgccgttcgg gcacaaggcg aagagtgcgc acgacatgct ccgcgaggcg
     2281 cgcgtgatga cggcgctgaa gcccgtgtac cccgtcgtgc ccgcggtgct cgccatcggc
     2341 gacgatccat ccgtcatggg gtgcgagttc ttcgtgatgg agcggatcgc cggcatcatc
     2401 cctcgccagg acctgcccgc ggggctcggt ttcggcgagg cgcagacacg caggctctgc
     2461 ctcaacgtcg tggacaagct cgtggaattg cacgcgatcg acccgcaggc ggccggtctc
     2521 gcggacctcg gcaagggcga gggctatgtc cggcgccagg tcgagggctg gagcaggcgc
     2581 tttcgcgacg cgcgaaccga cgacgtgagc gacttcgagt ccgtcatggc ctggctcgcg
     2641 gtgaggcgcc cggaacgcga ggtcgccatc cgcgtgatcc acggcgacta ccgcttcgac
     2701 aacgtggtgc tcgaccccgc cgacccgttc agggtcatcg gcgttctcga ctgggagatg
     2761 gccaccctcg gcgacccgct catggacctc ggcaactcgc tcgcctactg ggtgcaggcc
     2821 gacgacgacg agtatttccg catgacccgg cgccagccca ccaacgcgcc ggggatgctc
     2881 acgcgcgcgc aggtgatcga gcactatgcg gcgcgcaccg gactgccggt cgcgaacttc
     2941 gacttctacg cggtcttcgg gctcttccgc gttgcggtga tcgttcagca gatctggcgc
     3001 cgctatcgcg agggtgccac gaaggacccg cagttcgccg ccttcggccc gctcgtcacc
     3061 tatctcgacg ggcgctgccg gaggctcgtc gccgattcgc acggtgtctg acacggtgtc
     3121 agacaccgtg tcagacaccg caggaggatt catgaaggag tttgcaggaa aggtcgccgt
     3181 gatcaccggc gcagccgagg gaatcggcag ggcagtggcc gaggaggcag ccgcgcgcgg
     3241 catgaagctg ctgcttgccg acatcgacgc ggcgaggctc gatgccacga cggcggaact
     3301 gcgcgcgaag ggcgccgatg ccgacggctt gcgaaccgac gtgtcgaagg cgagccaggt
     3361 cgacgacctg gcggacctcg cgttcgggcg attcggccgc gtgcacctgc tcgttgccaa
     3421 cgcgggcgtg gcctgtgcca agccggtgtg ggacaccagc ccggaggact gggagtgggt
     3481 gatgggcgtg aatttctacg gcgtcacgca cgtgttgcgg gccttcctgc cgcgcatgat
     3541 ggccggtggc gaagaagggc acgtggtggc gaccgcgtcg atggccggtc tgctgtcgct
     3601 tccgggcatg gcggcgtaca acgccagcaa gcacgccgtg gtgacggtga tggagggcct
     3661 gcatcacgac ctcaccttgc gcagcgcgaa gatcggtgcc tgcgtcctgt gcccgagctg
     3721 ggtgaagacg cgcatcgccc tctccgagcg caatcgcacg tcgggcgagg tcacgcggcc
     3781 cgaggacctc gatcccgtca cggcaaggct ggccgccgcg gtgcaacggg ccgtggacgg
     3841 ggggatcgag cccggcgagg cagcgcgggc cgtgttcgca gccgtttccg acggacgctt
     3901 ctacgtcctc acccatggcg actcgaagtc gggcgtgaag gcgcggctcg acgccatcct
     3961 gcacgaccgc gtaccccccc tcgtccgcct gtaggtcccg cctgccgccc cgtgcggacg
     4021 gcgggttcgc gcggaacctg ctcaatcgtt acgcagtagt gccgcccaag cacctgattt
     4081 cgcgcaggaa aaaaatcttg cccgcagcgc ttgcgcgcgc tgcgttcctt tactagactt
     4141 tgtggtcacg ctcgccaaca gacactagat cttgtggatg gagcggtgga caagttgtgc
     4201 gcaacctggg gaatcgttcc tggtcatccg caaggaaatc aggaccttgg atggtccccc
     4261 gcatagctgt gttttttcga cacgattgcc cgcggcgtcc cacgcggccc gcgggctcca
     4321 aaacagcttc atacaaggag aggacccatg cgcatggttt ccgacgtcaa gcctacgacc
     4381 ccccttcccg acccggtgca gagcgaggag ggcgtgaact tgccggagag gtacgcggac
     4441 tacaaggtca tccgccgcaa cggggcggtc gtcggcttcg agccggacaa gatcgccgtc
     4501 gcggtgacca aggcgttcct cgccgtcgcg ggcggcaccg cagccgcgag cgcgggtgtt
     4561 cgcgagaagg tgcatggcgt cacgcaggcc gtggtgaacg cgctcgtgcg ccgccatccg
     4621 gccggcggca cgtaccacat cgaggacatc caggaccaag tggaactcgc cctgatgcgc
     4681 gccggcgagc accaggtcgc gcggtcctac gtgctctatc gcgaggagcg cgcccgcgag
     4741 cgcgccgccc gcgcgcagga ggccgcccag gcgcgccagc cgaaactgca cgtgacggtg
     4801 aacggcggca aggtgccgct cgacctcgcc tccctcgagg ccctcatcgt ctccgcctgc
     4861 caggacctgg gcgatgcggt ggagcccggg acgatcctcg acgcgacgct gcgcgacctc
     4921 tacgacggcg tgcccatcga ggaagtgcac aagtgcgcca tcctcgccgc gcgcatgctg
     4981 atcgagcagg acccggcgta cacctacgtt tcggcgcggc tgctgctgca ctcgatccgc
     5041 cgcgaagtgc tcggcgagga agtcgcgcac gagcagatgg ccagccgcta cgccgagtac
     5101 ttcccgacct tcatccagca gggcatccgg gcgcagcttc tcgacgagcg cctcggcacc
     5161 ttcgacctcg tgcgcctggg cgaggcgatc cgctcctcgc gcgacctcaa gttcgactac
     5221 ctgggcctgc agacgctcta cgaccgcgac ttcctgcaca tccacgacca ccacatcgag
     5281 ctgccgcagg cgttcttcat gcgcgtggcc atgggcctgt ccatcaacga ggtgcaccgc
     5341 gaggagcgcg ccatcgagtt ctacaacgtg ctctcgacgt tcgacttcat gtcgagcacg
     5401 ccgacgctct tcaactccgg cacgcagcgc tcgcagctct cctcctgcta cctcaccacg
     5461 gtcgccgaca atctcgacgg catctacgag gccatcaagg agaacgcgct gctctccaag
     5521 ttcgcgggcg ggctgggcaa cgactggacg ccggtgcgcg cgctgggttc ctacatcaag
     5581 gggaccaacg ggaaatcgca aggagttgtc cctttcctga aagtagtgtc ggacaccgcc
     5641 gtcgcggtga accagtgctt cgcgccggag accggcgtct acaccgccga cggcctcaag
     5701 ccgatcaagg atgtgaagcc cggcgacctc gtgctgggca agtccggtca gtaccgcgag
     5761 gtcgagcgcc gattcgccta caaccagacg gacgccatgg ttgccgtgaa ggtgaagcac
     5821 gcaatcgatc cgttgctcgt cacgacgggt cacccgttct tcgccatcca gtgcgtgccg
     5881 atggaacagg cgaatagccg caccctcgcc tggctcgcca agggaaaggt gaaggcagag
     5941 tgggtcgaag ccgggcagct tcaggccggc gactacgtgg cgcaggtggt gccgacggaa
     6001 gtggtgaacg tgcccggcct ctcggaggac gacgcgcgct tctacggcat cctcctcggc
     6061 gacgggcacc tttcgaagga ttgcctggag tggggcgtct cgggcaatcc gctcaaggac
     6121 gagcacctcg cattcgtacg cgcctatctc gcggagcgcg gcatccatta ctgggaacat
     6181 cagcgtggcg aaaattacgg tcaggtccac tgggcctcgg gccgcggcgt ggtgcgcaac
     6241 ggcaccaccg gccgcattgt cggcgcgggc gcgcccacga tgcccttcgg ccacgaggat
     6301 ctctatgacg aggcgggcag gaaacacgtc gcgcgccgct ttgcccacct gccgaaggcg
     6361 catacgctag ccatgatccg cggtctcctc gaaaccgatg gcggcgtctc gcgcggcaag
     6421 gagatctatt tcaccaatac ctcgaagccg ctcgtcgacg gactgcgcta tcagcttctg
     6481 cgcatgggcg tgccgacggc cgggcagtac cgcgagcgtg agaacggtca caccgccacg
     6541 cgttccgacg gctccacgac caccttcgtc ggtaccacga aggcctacga cctgcgcatt
     6601 ccggcggttc ccgtgattgc cggcctcgtg ggttgcaagc cgatcgtgaa gaggaactgg
     6661 ctcgagatcg aaggcaagct cttctcccgt atcacggaga tccaggccat cacaccgaag
     6721 ccgttcgtct acgacctcaa ggtcgaaacc gacgagtcgt acatgacgga agccggcctt
     6781 gcgcacaacg gcggcaagcg aaagggggcg gtgtgctgct acctcgagac ctggcacctg
     6841 gacatcgagg aattcctgga gctgcgcaag aacaccggcg acgaccgccg ccgcacgcac
     6901 gacatgaaca c
//
LOCUS       DASUEV010000112        15443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1521999, whole genome shotgun sequence.
ACCESSION   DASUEV010000112 DASUEV010000000
VERSION     DASUEV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521999"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1574)
                     /gene="lpdA"
                     /locus_tag="VFV55_03660"
     CDS             complement(144..1574)
                     /gene="lpdA"
                     /locus_tag="VFV55_03660"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX4943423.1"
                     /translation="MSQTFDVVVIGAGPGGYIAAVRAAQLGLKTACIDGWKNAKGELA
                     LGGTCLNVGCIPSKALLASSEEFEKVNHHLDEHGITVAGAKIDVARMQARKDGIVTKM
                     TKGIEFLFKKNKITWLQGFGKFVAAGDLYTVEVANGDRKETVQAKNVIVATGSKARHL
                     PNVPVDNVTICDNEGALAFGSAPKRLGVIGAGVIGLELGSVWRRLGSEVTILEALPVF
                     LGVCDEAVQKEAWKTFTKSQSLNIRLGVRIEKVSVRKIGVLVEYTDDKGLAQKLECDK
                     LVVSVGRLPNTDGLGALNVGLKVDERGFVEVDGQCRTNLPNVWAIGDVVRGPMLAHKA
                     EDEGVMVAELIAGQKPHIDYDCIPWIIYTSPEVAWVGLTEQKAKADGIAVRTGQFPFA
                     INGRALGSGNAQGFVKIVADAATDRVLGVHIVHAHASDLIQEAVTVMEFKGASEDIAR
                     ICHGHPTLSEVVKEAALAVDKRSLNS"
     gene            complement(1693..1986)
                     /locus_tag="VFV55_03665"
     CDS             complement(1693..1986)
                     /locus_tag="VFV55_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943424.1"
                     /translation="MARRRGKWRPAIVLRRAFFFVLGSLLVGFLAVLFQQSLGLYLPR
                     HVAVSLAVGLAVAATFWLLGLLKHPPLFLRNVPPLAAGLCAGLGVHIARFWLR"
     gene            complement(1997..3220)
                     /gene="odhB"
                     /locus_tag="VFV55_03670"
     CDS             complement(1997..3220)
                     /gene="odhB"
                     /locus_tag="VFV55_03670"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX4943425.1"
                     /translation="MQVEVKVPQLSESVSEATLVAWHRKVGEAVKRDENLIDIETDKV
                     VLELPAPADGVIAKIVREDGSTVTSGEVIAMIDTAGAVAASAPAVDPAQAARSAAPAA
                     AAAGAKAASPAAAKLAAEKGVDTAALGGTGRDGRITKGDVLAAASPAAPAPAAPAPAA
                     PSAKPAAPVSIPVGNRPEQRVPMSRLRARVAERLVQSQSTAAILTTFNEVNMAPVMEL
                     RNRYKDRFEKEHGVKLGFMSFFVKAAVAALRKYPVVNASIDGTDIVYHGFFDVGIAVG
                     SERGLVVPILRNADQMSIADIEKAIADFGKRAKDGKITIEELTGGTFSISNGGVFGSM
                     LSTPIINPPQSAILGIHATKERAVVENGQVVVRPMNYLALSYDHRIIDGREAVLSLVA
                     MKDALEDPARLLLDV"
     gene            complement(3253..6099)
                     /locus_tag="VFV55_03675"
     CDS             complement(3253..6099)
                     /locus_tag="VFV55_03675"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX4943426.1"
                     /translation="MSSVMKQFEASAHLYGANAPFVEELYERYLRDPASVAPEWRAQF
                     DAWQAPEGRRDVAHTPVIEAFAAAAKRGPGASPTAVAADDEKQMKVLMFIRAHRTTGS
                     HYSNLDPLKRMDHVPVPELELASYGLGEADMGTVFSMGSFANRRELKPLREIVRMVRK
                     TYCDTIGVEYMYLSSMEEKRWLRDRFEGTLSTPKVSAEEKRFLLERLTASETLERYLH
                     TRYVGQKRFSGEGGESLVPLLDIVIEEGGAQGLKEVVIGMAHRGRLSVLVNNLGKIPA
                     DLFSEFEGKKAAELAAGDVKYHQGFSSDIQTAGGAVHLTLAFNPSHLEIVDPVVEGSV
                     RARQVRRGDLKGDTVLPLLIHGDASFSGQGVVMETLALSQTRGYRTGGTLHVVVNNQI
                     GFTTSDTRDTRSSLYCTGIAKMIEAPVFHVNGDDPEAVAMVARIALDYRMKFRKDVVI
                     DMVCFRRLGHNEQDEPFVTQPLMYKKIAQHPGTRKLYADRLEREGVVAPGLADELVTS
                     VRTALDAGKPTNPKILYGLRPALAVDWAPYMGVDWRRPATTALPLEKLKQLAVRLTDI
                     PSNFKLHPTVERMLAARREMGQGRLALDWGMAENLAYASLVDEGHPVRLSGQDCGRGT
                     FAHRHAVLHDQNREKWDSGVYLPLQHIREGQGNFLVIDSLLSEEAVLGFEYGYATAHP
                     FELVIWEAQFGDFANGAQVVIDQFIASGEAKWGRLCGLTMFLPHGYEGQGPEHSSARL
                     ERYLQLCAEHNIQVVVPSTPAQFFHMLRRQILRPLRKPLIVMTPKSLLRRKESTSPLE
                     DLAGGAFQCVIGDPAKPAPKKVKRVVFCSGKVYFDIAAERDKRGIDDVALVRVEQLYP
                     FPHADFAEQIALYPSAKSVVWAQEEPANQGAWHRIQHYLLEHLRPDQVLGAAQRKSSA
                     SPAVGYLALHNQQQKELIDAALTLDAASSRKAGD"
     gene            complement(6137..7444)
                     /gene="gltA"
                     /locus_tag="VFV55_03680"
     CDS             complement(6137..7444)
                     /gene="gltA"
                     /locus_tag="VFV55_03680"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX4943427.1"
                     /translation="MQAKGKALLTYDDGKTLEMPVYGGTIGPDAIDIRALYGKTGKFT
                     YDPGFLSTASCNSTITYIDGDAGVLMYRGYPIEQLAKNCDFLEVCYLLLRGELPNGSQ
                     KKTFDRTVTLHTMVHEQLARLYQGFRRDSHPMAVMVGVVGALSAFYHDSLDINDPEHR
                     EISAFRLIAKLPTIVAMAYKYNMGQPFMYPKNDLDYSSNFMRMMFGVPAEDYHVNPVL
                     VRALDRILILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALQ
                     MLMDIQKEGGVEKLGEFVKKAKDKNSGVKLMGFGHRVYKNYDPRAKLMQETCREVLDE
                     LGLHNDPLFKLAMALEKVALEDEYFVSRKLYPNVDFYSGIVQRALGIPVSMFTCIFSL
                     ARTVGWIAQWNEMISDPELKIGRPRQLYQGATKRDVVPLAKRG"
     gene            complement(7466..7717)
                     /locus_tag="VFV55_03685"
     CDS             complement(7466..7717)
                     /locus_tag="VFV55_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX4943428.1"
                     /translation="MIEAVRSRELERLKWRSRRGLLELDVVLARFWGGCGEGLDDTDA
                     VRLATLLAMPDNDLLDLVMGRRDCPDPALKPLLQRLKAA"
     gene            complement(7718..8413)
                     /locus_tag="VFV55_03690"
     CDS             complement(7718..8413)
                     /locus_tag="VFV55_03690"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX4943429.1"
                     /translation="MKFRIHRYDPDKDPRPYFQDYDVAMGPADRMLLDALVRIKSVDD
                     TLSLRRSCREGVCGSDAMNINGKNGLACVTKLAGLAEPVVIKPLPGLPIIRDLIVDMT
                     QFFDQYHSVKPYVIDPTPLPEKERLQSPRERAELDGLYECILCACCSTSCPSFWWNPD
                     KFVGPAGLLNAYRFIADSRDQALNERLDDLEDPYRLFRCHSIMNCVDVCPKGLNPTLA
                     IGRIKELMVRRAV"
     gene            complement(8415..10181)
                     /gene="sdhA"
                     /locus_tag="VFV55_03695"
     CDS             complement(8415..10181)
                     /gene="sdhA"
                     /locus_tag="VFV55_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX4943430.1"
                     /translation="MALPVRKFDAVIVGAGGSGLRAALELSNAGLKVAVLSKVFPTRS
                     HTVAAQGGVGASLGNMGEDSWHWHMYDTIKGSDWLGDQDAIEFMCRMAPEVVIELEHF
                     GMPFDRNENGTIYQRPFGGHTQNFGERPVQRSCCAADRTGHAMLHTLYQRNVLARTQF
                     FVEWMALDLVRDAGGAVLGVSALEMETGDVVLFQGKATLFATGGSGRMFASSTNAFIN
                     TGDGLGIAARAGIPLQDLEFFQFHPTGVFGAGILITEAVRGEGGILLNKNGERFMERY
                     APTMKDLASRDVVSRAMATEIKEGRGAGEHADHILLKLDHLGPDVIDRRLPGIREIAL
                     KFANVDCAKEPIPVVPTAHYQMGGIPTNYRGEVVVPKDGNPASPVPGFYAAGECGCAS
                     VHGANRLGTNSLTDLLVFGKSAGMSMIDHVRRHAGAHPDLPQDAGDRSLARLARLEGQ
                     KGGEDVSAVRDELQRAMQAHCGVFRFPDLLRQGVERIRALGERLARTEVKDKSKVFNT
                     ARIEALELENLYEAARATMVGAEARKESRGAHDREDFHARDDQGWLKHTLWYKEGDRL
                     DYKPVNLKPLSVESFEPKARVY"
     gene            complement(10183..10536)
                     /gene="sdhD"
                     /locus_tag="VFV55_03700"
     CDS             complement(10183..10536)
                     /gene="sdhD"
                     /locus_tag="VFV55_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX4943431.1"
                     /translation="MKAYAKNPVGAHYGLSAWLLQRLTAVVMATYTVFMLVFVLWCPP
                     ASWADWKGMFSGSFVRLATMLFFLALLYHAWVGVRDIIMDYVKPTGVRLALQALVGTA
                     LVFYLVWSVSILWGR"
     gene            complement(10533..10919)
                     /gene="sdhC"
                     /locus_tag="VFV55_03705"
     CDS             complement(10533..10919)
                     /gene="sdhC"
                     /locus_tag="VFV55_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX4943432.1"
                     /translation="MTTAPKQRPKYYDLNLAHLPVPGLASIVHRISGALLFFPAIPAL
                     LFLMQSTLGTEEGWQHWKEVLAQPAVKVIVLGFVWLYAHHFFAGIRYLLLDLHVGVEL
                     DRARASAKIVFALGAVATLLIGWRLW"
     gene            complement(11095..11829)
                     /locus_tag="VFV55_03710"
     CDS             complement(11095..11829)
                     /locus_tag="VFV55_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4943433.1"
                     /translation="MTETLPSPSFRPLYEQIKILITQSLVAGEWKPGEAIPSEMEFAA
                     RFRVSQGTVRKAIDELASEHILVRRQGKGTFVASHSEPSYQYRFLRVMPDNGQKLHPH
                     SVLLEVRKGKASGEIARSLAIKTGSPVHMIKRLLEFQGRPLILDEMVLAASRFPGLTV
                     AKLEECKGSMYSFYETAFGLRMIRAEERIRAVPAEEAVAEHLRVPPGTPLLCVDRIAF
                     TYGDMPVEWRRGLCLTDGFSYYNELA"
     gene            12020..12898
                     /locus_tag="VFV55_03715"
     CDS             12020..12898
                     /locus_tag="VFV55_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX4943434.1"
                     /translation="MPPYFRPRRSMLYVPGCSMRYLNKSRTLKVDSVILDLGDPILVE
                     AKVESRRNVVEAVLAGGFGRREVVVRVNDLDSPWGEDDVKAVAGIGADAVLFPNVESR
                     ADVLQALSALDAAGGSHLPIMVMVESPLAVLRAEEIAAASDRIACIVMATSDLITQLH
                     ARRTPDRIALHYSLSRVLLAGRAFDRSVVDGIATDLKDMQSFEYACRLARDLGFDGKS
                     LVHPFQLPYCNDAFTPKPHDLAAATELIEALEHAHREGRGVVVVNGRLIEGHHLKAAR
                     RLLALADMIQQLEAGR"
     gene            12903..13994
                     /locus_tag="VFV55_03720"
     CDS             12903..13994
                     /locus_tag="VFV55_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4943435.1"
                     /translation="MADRIARPLAGFFFDDYAIGQHLAHATPRTVTQADATLHLALTG
                     SRNPVHCAAPVAAALGHRAVPLDDLLVFHVAFGKTVHDVSFNAVANLGYADLRFLAPV
                     YPGDTLSARSEVIGLKENSSGKTGVVWVRSRAFNQDGAEVLSWVRWVMVARNLAVTAS
                     GKEGPAIPAPAAIVPHLPREVTAGRLSIPAFLDPRALDPRATGGTRWWDDYAPGETID
                     HPGAMTIEEADHMLATRLYQNTARVHFDALAARGTAFGRRLVYGGHVISLCRALAHDG
                     LENAFAIAAIHGGAHANPTFAGDTIVTRTVIHSRDEIPARADVGALRVRMLGAKNVKL
                     ADLATPAPGAGDPAVVLDLDLTLLVPRRA"
     gene            14032..15024
                     /locus_tag="VFV55_03725"
     CDS             14032..15024
                     /locus_tag="VFV55_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4943436.1"
                     /translation="MPPPRHPKDALFAGEKPFPVIPACEHFAGSEKLITKALDLQERL
                     GAVFDITCDCEDGAETGREKQHAQMIVDLLHSSANRFAMAGARIHDFTHPHWKKDVDI
                     LVGGAGQVIAYVTIPKCTSVVQAEEMILYVQSVARRRKIKRAIPVHVLIETHGALHDA
                     WEIAQLPGVQVLDFGLMDFVSGHHGAINASNMRTPGQFEHPLVARAKVDVVAAALANG
                     VVPSHNVSLDLKNSYNVFRDAWRARSDFGFLRMWSIHPSQVQPIVDAMKPDLNEVATA
                     GAILLEAQKASWGPIQFEGELHDRATYRYFWELLQKARITGVKMPEDAARAFFD"
     gene            15115..>15443
                     /locus_tag="VFV55_03730"
     CDS             15115..>15443
                     /locus_tag="VFV55_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX4943437.1"
                     /translation="MTTMTPGAKFRAALKDEKPLQVPGAICAYHATLAKASGFRAIYL
                     SGGGVAAGSLGIPDLGITNLDDVLTDIRRITDVCDLPLLVDVDTGFGASAFNVARTTK
                     SLIKFGAA"
BASE COUNT         2436 a         5272 c         5174 g         2561 t
ORIGIN      
        1 aacacggata agagcagatg aaattgtttc gggattcggg ggaagcccgt cgcgagcccg
       61 gaagacgctc tttatcagct cttatccgtg ttcatccgtg ttcatccgtg tccaacgctc
      121 ttggcttccg tcagcccctg cgcctacgag ttgagggaac gcttgtcgac cgccagcgcc
      181 gcttccttca ccacctcgga aagcgtcggg tgcccgtggc agatgcgcgc gatgtcctcc
      241 gacgcgccct tgaactccat taccgtcacc gcctcctgga tcaggtcgga ggcgtgcgca
      301 tgcacgatgt gcacgcccag aacgcggtcc gtggcggcgt cggcaacgat cttcacgaag
      361 ccctgcgcat tcccgcttcc cagcgcgcgg ccgttgatcg cgaagggaaa ctgccccgtc
      421 ctcaccgcga tgccgtccgc cttcgccttc tgctcggtca ggcccaccca cgccacctcc
      481 ggcgaggtgt agatgatcca cgggatgcag tcgtagtcga tgtgcggctt ctggccggcg
      541 atgagttccg cgaccatcac gccctcgtcc tcggccttgt gcgcgagcat cggcccgcgc
      601 accacgtcgc cgatggccca cacgttcggc aggttcgtgc ggcactgtcc gtcgacctcg
      661 acgaatccgc gttcgtccac cttgaggccc acgttcaagg cgcccaggcc gtcggtgttg
      721 ggcaggcgcc cgacggagac cacgagcttg tcgcactcga gcttctgggc gaggcccttg
      781 tcgtccgtgt attccaccag gacgccgatc ttgcgcaccg agaccttctc gatcctgacg
      841 cccagcctga tgttgagcga ctgggacttg gtgaaggtct tccaggcctc cttctgcacc
      901 gcctcgtcgc acacgcccag gaaaaccggc agcgcctcca ggatcgtcac ctccgagccc
      961 agccgccgcc agacgctgcc cagttccaga ccgatgacgc ccgcgccgat gaccccgagc
     1021 cgcttgggcg cggacccgaa ggcgagcgcg ccttcgttgt cgcagatggt cacgttgtcc
     1081 accggcacgt tcggcaggtg gcgcgccttc gacccggtgg cgacgatgac gttcttcgcc
     1141 tgcaccgtct ccttcctgtc gccgttcgcc acctcgacgg tatagaggtc tcctgcggcc
     1201 acgaacttcc cgaagccctg cagccaggtg atcttgttct tcttgaacag gaattcgatt
     1261 cccttggtca tcttcgtgac gatgccgtcc ttgcgcgcct gcatcctggc cacgtcgatc
     1321 ttggcccccg cgaccgtgat gccgtgctcg tcgaggtggt ggttcacctt ctcgaattcc
     1381 tcggacgagg cgaggagcgc cttggacggg atgcacccga cgttgaggca cgttccgccg
     1441 agggccagct cgcccttcgc gttcttccag ccgtcgatgc aggcggtctt gagtcccagc
     1501 tgggcagcgc gcaccgcggc gatgtagccg ccgggcccgg caccgatcac gacgacgtcg
     1561 aaggtctgcg acatggcggt tccgatcgag tgaagtgggt tacgggttcg acaccgggga
     1621 caaggcggcg atggggggag gccagatgct cgacacggag aacccggcgc tcgtcagtca
     1681 cgcgttgcgt ccctagcgca gccagaagcg cgcgatgtgc acgcccagcc ccgcgcaaag
     1741 gcccgccgcg agaggcggaa cattcctgag gaagagcggc gggtgcttga ggagccccag
     1801 cagccagaag gtggccgcca ccgccagtcc caccgcgagc gacaccgcga cgtgccgcgg
     1861 caggtacagg cccagcgatt gctggaagag gaccgcgagg aagcccacga ggagcgaccc
     1921 cagcacgaag aagaaggccc gccgcaggac gatcgcgggc cgccacttcc ccctgcgccg
     1981 tgccaatttc ccgctcctag acgtcaagca gcaggcgcgc cgggtcctcc agcgcgtcct
     2041 tcatggccac cagcgacagc accgcctcgc gtccgtcgat gatgcggtgg tcgtaggaga
     2101 gcgcgaggta gttcatcggc cgcaccacca cctgcccgtt ctcgacgacc gcgcgttcct
     2161 tggtggcgtg gatgccgagg atggcgctct gcggcgggtt gatgatcggc gtcgagagca
     2221 tcgagccgaa gaccccgcca ttggagatcg agaaggttcc cccggtcagt tcctcgatgg
     2281 tgatcttgcc atccttggcg cgcttgccga aatccgcgat cgccttctcg atgtcggcga
     2341 tcgacatctg gtcggcgttg cgcaggatcg gcaccaccag cccgcgctcg ctgcccaccg
     2401 cgatgcccac gtcgaagaag ccgtgataga cgatgtcggt gccgtcgatg gaggcgttca
     2461 ccaccgggta cttgcgcagc gcggccaccg cggccttcac gaagaaggac atgaagccga
     2521 gcttcacgcc gtgctccttc tcgaagcggt ccttgtagcg gttgcgcaac tccatcaccg
     2581 gcgccatgtt gacttcgttg aacgtggtga ggatggcggc cgtggactgc gactggacga
     2641 ggcgttcggc gacgcgcgcg cgaagccgcg acatcggaac gcgctgttcg ggccggttgc
     2701 cgacgggaat ggagaccggc gcagcgggct tcgccgaagg tgccgcgggc gcgggtgctg
     2761 caggcgcagg cgccgccggt gaagcggcag cgagcacgtc gcctttcgtg atgcggccgt
     2821 cgcgacccgt gccgccgagt gcggcggtgt ccacgccctt ctccgcggca agcttggccg
     2881 ctgcagggga cgcggctttc gcgccggccg cggcggcagc cggggcggcg gacctcgcgg
     2941 cctgcgcggg gtcgacggcc ggtgccgagg ccgccacggc gcccgccgtg tcgatcatgg
     3001 cgatcacctc gcccgaggtc acggtgctgc cgtcctccct gacgatcttc gcgatcaccc
     3061 cgtcggcggg cgcgggcaat tccagcacca ccttgtcggt ctcgatgtcg atcaggttct
     3121 cgtcgcgctt caccgcctcg cccaccttcc tgtgccaggc gacgagggtg gcctccgaga
     3181 cgctctcgga caactggggg accttcactt cgacttgcat gtcgtttcct tcgctgcgcg
     3241 ccggcggacc cttcagtccc cggccttcct ggaggaagcg gcgtccagcg tgagggccgc
     3301 gtcgatgagt tccttctgct gctggttgtg cagcgccagg tagccgacgg cgggcgacgc
     3361 cgacgacttg cgctgcgcgg cacccaggac ctggtcgggc ctcaggtgct ccagcaggta
     3421 atgctggatg cggtgccacg cgccctggtt ggccggttcc tcctgcgccc acaccacgct
     3481 cttcgcgctg gggtagaggg cgatctgttc ggcgaaatcg gcgtgcggga acgggtagag
     3541 ctgctccacg cgcacgaggg ccacgtcatc gatgccgcgc ttgtcgcgct cggccgcgat
     3601 gtcgaaatag accttgcccg agcagaacac gacgcgcttc accttcttcg gggcgggctt
     3661 cgcgggatcg ccgatcacgc actggaaagc gccgccggca agatcctcga gcggcgaagt
     3721 cgattccttg cgcctgagca ggctcttggg cgtcatcacg atgaggggct tgcgcaacgg
     3781 ccgcaggatc tgccggcgca gcatgtggaa gaactgcgcg ggcgtggacg ggacgacgac
     3841 ctggatgttg tgctcggcgc acagctgcag gtagcgctcc aggcgcgccg aggagtgctc
     3901 ggggccctgc ccttcgtagc catgcggcag gaacatcgtg aggccgcaca ggcgccccca
     3961 cttcgcctcc ccggaggcga tgaactggtc gatcaccacc tgcgcgccgt tggcgaagtc
     4021 gccgaactgc gcctcccaga tcaccagctc gaagggatgc gccgtcgcgt agccgtactc
     4081 gaatccgagg acggcctcct ccgacagcag cgaatcgatc accaggaagt tgccctggcc
     4141 ctcgcggatg tgctgcagcg gcaggtacac gccggaatcc catttctccc ggttctggtc
     4201 gtgcagcacg gcgtggcggt gcgcgaaggt gccgcgcccg caatcctggc ccgacaggcg
     4261 caccgggtgg ccctcgtcca cgagcgaggc gtaggcgagg ttttccgcca tgccccagtc
     4321 gagggcgagc ctgccctggc ccatctcgcg ccgcgcggcg agcatgcgct cgaccgtcgg
     4381 atgcagcttg aagttcgacg ggatgtcggt caggcggacg gcgagctgct tgagcttttc
     4441 caggggcagc gccgtggtcg ccgggcggcg ccagtcgacg cccatgtagg gcgcccagtc
     4501 gacggcgagc gccggcctga ggccgtagag gatcttggga ttcgtgggct tgcccgcgtc
     4561 cagcgcggtg cgcacgctcg tcaccagctc gtcggcgagg ccgggggcga cgacgccctc
     4621 gcgctcgagc ctgtccgcgt agagcttgcg cgtgcccggg tgctgcgcga tcttcttgta
     4681 catgagcggc tgggtgacga agggctcgtc ctgctcgttg tggcccaggc gccggaagca
     4741 caccatgtcg atcaccacgt ccttgcggaa cttcatgcgg tagtcgagcg cgatccgcgc
     4801 caccatcgcc accgcctcgg ggtcgtcgcc gttcacgtgg aagacgggcg cctcgatcat
     4861 cttggcgatg cccgtgcagt agagcgagga tcgggtatcg cgcgtgtccg aggtcgtgaa
     4921 gccgatctgg ttgttcacga cgacgtgcag cgtgccgccg gtccggtagc cgcgcgtctg
     4981 cgacagggca agtgtctcca tcaccacgcc ctgcccggaa aaggacgcgt cgccgtggat
     5041 gagcagcgga agcaccgtgt cacccttgag gtcgccgcgg cgaacctggc gcgcgcgcac
     5101 cgagccctcc accaccgggt ccacgatctc caggtgcgag gggttgaagg cgagcgtgag
     5161 atggaccgcg ccgcccgcgg tctggatgtc cgaggaaaat ccctggtggt acttcacgtc
     5221 gccggcggcg agctcggcgg ccttcttgcc ctcgaactcg gaaaacaggt cggcgggaat
     5281 cttgccgagg ttgttgacca ggacgctgag gcgcccgcgg tgcgccatgc cgatcaccac
     5341 ctccttgagt ccctgcgcgc cgccctcctc gatcacgatg tcgagcagcg gcaccaggct
     5401 ctcgccgcct tcgccggaga agcgcttctg gcccacgtag cgcgtgtgca ggtagcgctc
     5461 cagcgtctcc gaggcggtga ggcgctcgag caggaagcgc ttctcctcgg ccgacacctt
     5521 cggcgtggag agcgtgccct cgaagcggtc gcgcagccag cgcttctcct ccatggacga
     5581 gaggtacatg tactcgacgc cgatcgtgtc gcagtacgtc ttgcgcacca tccggacgat
     5641 ttcgcgcaac ggcttcagct cgcgccggtt ggcgaaggac cccatggaaa acacggtgcc
     5701 catgtccgcc tcgcccagcc cgtaggatgc gagctcgagc tcgggcacgg gcacgtggtc
     5761 catgcgcttg agcgggtcga ggttcgagta gtgcgagccg gtggtgcggt gcgcccggat
     5821 gaacatcagc accttcatct gcttctcgtc gtcggcggcg accgccgtcg gcgaggcccc
     5881 gggcccgcgc ttcgccgccg ccgcgaaggc ctcgatcacc ggcgtgtggg cgacgtcgcg
     5941 cctgccctcc ggggcctgcc atgcgtcgaa ctgcgcgcgc cactccgggg cgaccgatgc
     6001 cgggtcccgg aggtagcgct cgtagagttc ctcgacgaac ggcgcgttgg cgccgtacag
     6061 gtgcgcggat gcctcgaact gcttcatgac gctggacatt ggggcctcgc gtggcgggaa
     6121 cggggtcctt cgggcgctag ccgcgcttgg cgagcggaac gacgtcgcgc ttggtggccc
     6181 cctggtagag ctggcgcggc cggccgatct tcagctccgg gtccgagatc atctcgttcc
     6241 actgcgcgat ccagcccacc gtgcgcgcga gcgagaagat gcaggtgaac atggacaccg
     6301 ggatgcccag cgcgcgctgc acgatgccgg aatagaagtc gacgttgggg tagagcttgc
     6361 ggctgacgaa gtattcgtcc tcgagcgcca ccttctccag cgccatcgcg agcttgaaca
     6421 gcgggtcgtt gtgcaggccc agctcgtcga gaacctcgcg gcaggtctcc tgcatcagct
     6481 tggcgcgcgg gtcgtagttc ttgtacacgc ggtggccgaa gcccatgagc ttcaccccgg
     6541 aattcttgtc cttcgccttc ttcacgaact cgccgagctt ctcgacgccg ccttccttct
     6601 ggatgtccat gagcatctgc agcgccgcct cgttggcgcc gccgtgggcc gggccccaga
     6661 ggcaggcgat gcccgcggcg atgcaggcga agggattggc gccggaggag cccgccagcc
     6721 gcaccgtcga ggtcgaggcg ttctgctcgt ggtcggcgtg caggatgagg atgcggtcga
     6781 gcgcgcgcac cagcaccggg ttcacgtggt agtcctcggc cgggacgccg aacatcatgc
     6841 gcatgaagtt cgacgagtaa tcgaggtcgt tcttgggata catgaacggc tggcccatgt
     6901 tgtacttgta ggccatggcg acgatcgtcg gcagcttcgc gatgaggcgg aaggcggaga
     6961 tctcgcggtg ctcggggtcg ttgatgtcga gggagtcgtg gtagaaggcc gagagcgcgc
     7021 ccaccacgcc gaccatcacc gccatcgggt gggagtcgcg gcggaacccc tggtacaggc
     7081 gcgcgagctg ctcgtgcacc atcgtgtgca gggtgacggt ccggtcgaag gtcttcttct
     7141 gcgagccgtt cggcagctcg ccgcgcagga gcaggtagca gacctccagg aagtcgcagt
     7201 tcttggcaag ctgctcgatc gggtagccgc ggtacatcag cacgcccgcg tccccgtcga
     7261 tgtaggtgat ggtcgagttg cagctcgccg tcgacaggaa gcccggatcg taggtgaact
     7321 tgccggtctt gccatacagg gcgcggatgt cgatcgcatc cgggccgatg gttccgccat
     7381 acaccggcat ttccagcgtc ttgccgtcgt cgtaggtcag tagtgccttg cctttggctt
     7441 gcatgcgttt ctccaggtgg gccgcctagg cggccttgag tcgttgcagt agcggtttca
     7501 gtgccggatc ggggcaatcg cgccgcccca tcaccaggtc gagcaggtca ttgtccggca
     7561 tcgcgagaag cgtcgcgagg cgcaccgcgt ccgtatcgtc cagtccttcc ccgcatcccc
     7621 cccagaaccg cgcgagcacg acatcgagct cgagaagccc ccggcggctg cgccatttga
     7681 gccgttccaa ttcgcgcgaa cgcacggctt cgatcattta taccgccctg cggaccatca
     7741 gttccttgat ccttccgatg gccagggtcg ggttgagccc cttcgggcag acatccacgc
     7801 aattcatgat ggaatggcag cggaagagcc ggtagggatc ctccaggtcg tcgagccgct
     7861 cgttgagcgc ctggtcgcgg ctgtcggcga tgaaccggta ggcattgagc agccccgcgg
     7921 gcccgacgaa cttgtcggga ttccaccaga aagacgggca ggacgtggag cagcaggcgc
     7981 aaaggatgca ctcgtagagc ccgtcgagct cggcgcgctc ccgcggcgac tgcaggcgct
     8041 ccttttccgg caggggcgtg gggtcgatga cgtagggctt gaccgagtgg tactggtcga
     8101 agaactgcgt catgtcgacg atcaggtcgc ggatgatggg caatcccgga agcggcttga
     8161 tcaccaccgg ctcggcaagc ccggcgagct tcgtcacgca cgcgagcccg ttcttgccgt
     8221 tgatgttcat cgcgtcggac ccgcagacgc cctcgcggca cgagcggcgc agcgacagcg
     8281 tgtcgtccac cgacttgatg cgcaccagcg cgtcgagcag catgcggtcg gccggcccca
     8341 tggccacgtc gtagtcctgg aagtagggcc tggggtcctt gtccgggtcg tagcggtgga
     8401 tgcggaattt catgtcagta gacgcgcgcc ttgggttcga aggattccac cgagaggggc
     8461 ttcaggttga ccggcttgta gtcgaggcgg tcgccctcct tgtaccagag cgtgtgcttg
     8521 agccagccct ggtcatcgcg ggcgtggaaa tcctcgcggt cgtgggcgcc gcgcgattcc
     8581 ttgcgcgcct ccgcgcccac catcgtcgcg cgggccgcct cgtagaggtt ctccagctcg
     8641 agcgcctcga tgcgcgcggt gttgaacacc ttcgacttgt ccttcacctc ggtgcgcgcc
     8701 agccgctcgc ccagcgcgcg gatccgttcc acgccctggc gcagcaggtc cggaaagcgg
     8761 aacacgccgc agtgcgcctg catcgcgcgc tggagctcgt cgcgcaccgc ggagacgtcc
     8821 tccccgccct tctgcccttc caggcgcgcg aggcgggcaa gcgaacggtc cccggcgtcc
     8881 tgcggcaggt ccgggtgcgc tccggcgtgc ctgcggacat ggtcgatcat gctcatgccc
     8941 gcgctcttgc cgaagaccag gaggtcggtg agggagttgg tcccgagccg gttcgcgccg
     9001 tggacggatg cgcagccgca ctcgcccgcc gcgtagaagc cgggcacggg gctcgccgga
     9061 ttgccgtcct tcggcacgac gacctcgccg cggtaattgg tcgggatgcc gcccatctgg
     9121 tagtgggccg tcggcaccac cgggatcggc tccttcgcgc aatccacgtt ggcgaacttg
     9181 agcgcgatct cgcggatgcc gggcagccgc cggtcgatca cgtcggggcc gagatggtcg
     9241 agcttgagaa ggatgtggtc ggcgtgctcg ccggcgccgc ggccttcctt gatctcggtc
     9301 gccatggcgc gcgacaccac gtcgcgggag gcgaggtcct tcatggtggg cgcatagcgc
     9361 tccatgaagc gctcgccgtt cttgttcagc aggatcccgc cttcgccgcg cacggcctcg
     9421 gtgatgagga ttccggcacc gaagacgccc gtcgggtgga actggaagaa ctcgagatcc
     9481 tgcagcggga tgccggcgcg ggcggcgatc cccagcccgt cgcccgtatt gatgaaggcg
     9541 ttggtggacg aggcgaacat gcgccccgag ccgccggtcg cgaagagcgt ggcctttccc
     9601 tggaagagca cgacgtcgcc cgtctccatc tcgagggcgg aaacaccgag gaccgcgccc
     9661 ccggcgtcgc gcaccaggtc gagggccatc cactcgacga agaactgtgt cctggcgagg
     9721 acgttcctct ggtagagggt atgcagcatg gcgtgcccgg tgcggtccgc ggcgcagcag
     9781 gagcgctgca cggggcgctc cccgaagttc tgcgtgtgcc cgccgaacgg gcgctggtag
     9841 atggtgccgt tctcgttgcg gtcgaaaggc atgccgaagt gctcgagctc gatcaccacc
     9901 tcgggcgcca tgcggcacat gaactcgatc gcgtcctggt cgcccagcca gtccgacccc
     9961 ttgatcgtgt cgtacatgtg ccagtgccag ctgtcctcgc ccatgttgcc gagcgaggca
    10021 cccacgccgc cctgcgccgc cacggtgtgc gagcgcgtcg ggaagacctt ggagaggacg
    10081 gcgaccttga ggccggcgtt ggagagctcg agcgccgcgc gcagtccgga accgccggcc
    10141 cccacgatca ccgcgtcgaa tttgcgaacc ggaagcgcca tgtcatcggc cccagaggat
    10201 ggatacggac cacacgaggt agaacacgag cgcggtgccc acgagcgcct ggagcgcgag
    10261 gcgcacgccc gtgggcttca cgtagtccat gatgatgtcg cgcacgccga cccacgcgtg
    10321 gtagaggagc gcaaggaaga agagcatcgt cgcgagccgc acgaaggagc ccgagaacat
    10381 ccccttccag tcggcccagc tcgccggcgg gcaccacagc acgaacacca gcatgaagac
    10441 ggtgtaggtc gccatgacca ccgcggtgag gcgctgcagc agccacgcgc tgagcccgta
    10501 gtgcgcgccg acggggttct tcgcgtaggc cttcaccaga gcctccagcc gatgaggagc
    10561 gtggccaccg cgcccagcgc gaagacgatc ttggccgagg cgcgcgcgcg atccagctcc
    10621 accccgacat gcaggtcgag cagcaggtag cggatgccgg cgaagaagtg gtgcgcgtag
    10681 agccagacga agccgagcac gatcaccttc accgccggct gcgcgagcac ctccttccag
    10741 tgctgccacc cttcttcggt gccgagcgtg gattgcatga ggaagagaag cgcggggatg
    10801 gcggggaaga agagaagcgc gccggagatg cggtgaacga ttgacgcgag ccccggtacg
    10861 ggcagatgcg cgagattgag atcgtaatac ttcggtctct gcttgggcgc agttgtcatt
    10921 gttcgttcct gggtggcatg gcggcggcca ggcgaaggcg ggaggggagc cgctgcacga
    10981 tcccgcgacg ctgaaagccg gggaattcta ccgtggattt gttgcgaccg cacaatcgga
    11041 tacccctgtc gtgcgcaccg aaattccact tttcccggaa tcgggaagcg cacctcacgc
    11101 gagctcgttg tagtaggaga atccgtccgt gaggcacagc ccgcggcgcc actcgacggg
    11161 catgtcgcca taggtgaagg cgatgcggtc cacgcacagc aggggcgtgc ccgggggcac
    11221 gcgcaggtgt tccgccacgg cctcctcggc gggcaccgcg cggatgcgct cctcggcgcg
    11281 gatcatgcgc agcccgaagg ccgtctcgta gaagctgtac atcgagccct tgcactcctc
    11341 gagcttcgcc accgtgaggc ccgggaagcg gctcgcggca agcaccatct cgtcgaggat
    11401 gaggggccgg ccctggaact ccagcaggcg cttgatcatg tgcaccggcg aacccgtctt
    11461 gatcgcgagc gagcgcgcga tctcgccgct cgccttgccc ttgcgcacct cgagcagcac
    11521 gctgtgcggg tgcagcttct gcccgttgtc cggcatcacg cgcaggaaac ggtactggta
    11581 ggaaggctcg ctgtgcgagg cgacgaacgt gcccttgccc tggcggcgca cgaggatgtg
    11641 ctccgaggcc agttcgtcga tcgccttgcg caccgttccc tggctcacgc ggaagcgcgc
    11701 ggcgaactcc atttcggagg ggatcgcctc tccgggcttc cattcgccgg ccaccaggct
    11761 ctgcgtgatg aggatcttga tctgctcgta gagcggccgg aacgacggcg acggcagggt
    11821 ctcggtcatg gcgcaaatgc tagcacagag ggtcgctcat atgtcttata taagataatg
    11881 tcttatataa gatacaagtc ctgttgacac ccctcgcggc cgcggcgtag catgctgcgc
    11941 agcagcaagc gcccccgatg tcgcttccgc cccgcgaccc gcaggcgcgc attccccgcc
    12001 aaatcagagg cttgccgcca tgccccccta cttccgcccg cgccgctcga tgctctacgt
    12061 gccggggtgc agcatgcgct acctgaacaa gtcgcgcacc ctgaaggtcg actcggtgat
    12121 cctcgacctc ggcgacccga tcctggtcga ggccaaggtc gagtcgcgcc gcaacgtggt
    12181 cgaggcggtt ctcgccggcg gcttcggccg gcgcgaagtg gtggtgcgcg tgaacgacct
    12241 cgactctccc tggggcgagg acgacgtgaa ggcggtggcg gggatcggcg cggacgcggt
    12301 cctcttcccc aacgtcgaat cccgggccga cgtgctgcag gcactttccg cgctcgatgc
    12361 ggccgggggc agccacctgc ccatcatggt gatggtcgag agccccctcg cggtgctgcg
    12421 cgccgaggag atcgccgccg catccgaccg catcgcctgc atcgtgatgg ccacgtcgga
    12481 cctcatcacg cagttgcacg cgcggcgcac gcccgaccgg atcgcgctgc actactcgct
    12541 ttcgcgcgtg ctgctggccg gccgcgcgtt cgaccgctcg gtggtcgacg ggatcgccac
    12601 ggacctgaag gacatgcagt ccttcgagta cgcctgccgg ctggcccgcg acctgggctt
    12661 cgacggcaag agcctcgtgc accctttcca gcttccctac tgcaacgacg ccttcacgcc
    12721 gaagccgcac gacctcgccg cggccaccga actgatcgag gcgctcgagc acgcgcatcg
    12781 cgaggggcgc ggcgtggtgg tggtgaacgg ccgcctcatc gaggggcacc acctcaaggc
    12841 cgcgcgccgg ctgcttgcgc ttgcggacat gatccagcag ctcgaagcgg gccgctgaca
    12901 ccatggcgga ccggatcgcc cggccgctgg ccggcttctt cttcgacgac tacgcgatcg
    12961 ggcagcacct cgcgcacgcc acgccgcgca cggtcacgca ggcggacgcc accctccatc
    13021 tcgctctcac cggcagccgc aatcccgtgc actgcgcggc cccagtcgcc gccgcgctcg
    13081 ggcaccgggc ggtgccgctc gacgacctgc tcgtcttcca cgtcgcgttc ggcaagaccg
    13141 tgcacgacgt ctccttcaat gccgtcgcca acctcggcta cgccgacctg cgcttcctcg
    13201 cccccgtcta tcccggcgac acgctttccg cgcggagcga agtcatcggc ctcaaggaga
    13261 actccagcgg gaagaccggc gtggtctggg tgcgctcgcg cgccttcaac caggacggtg
    13321 cggaggtgct ttcctgggtt cgctgggtca tggtcgcccg gaacctggcg gtaaccgcga
    13381 gcggcaagga ggggccggcc attcctgcgc ccgccgccat cgttccccac ttgccccgcg
    13441 aggtgacggc cggccggctg tcgattcccg cgttcctcga cccccgcgcg ctcgacccgc
    13501 gtgccaccgg cggaacccgc tggtgggacg actacgcgcc gggcgagacg atcgaccacc
    13561 ccggcgcgat gacgatcgag gaagccgacc acatgctcgc cacgcgcctt taccagaaca
    13621 ccgcgcgcgt gcacttcgac gcgctcgccg cccgcggcac cgccttcggc aggcgcctgg
    13681 tctacggcgg ccacgtgatc tcgctgtgcc gcgcccttgc ccacgacggc ctcgagaacg
    13741 cctttgcgat cgcggccatc cacggcgggg cgcatgccaa ccccacgttc gccggcgaca
    13801 cgatcgtcac gcgcaccgtg atccattcgc gcgacgagat accggcacgc gccgacgtgg
    13861 gcgcgctgcg cgtgcggatg ctcggcgcga agaacgtgaa gctcgccgac ctcgccacgc
    13921 ccgcccccgg cgcgggggat ccggcggtgg tcctcgacct cgacctcacc ctcctcgttc
    13981 cgcgccgcgc gtaacgccta gaatagccat ttcacttccg ccaggtagcc catgccccct
    14041 ccccgccacc cgaaggacgc ccttttcgcc ggcgagaagc cctttccggt catccccgcc
    14101 tgcgagcact tcgccggttc cgagaagctc atcaccaagg cgctcgacct gcaggaaagg
    14161 ctcggggccg tgttcgacat cacctgcgat tgcgaggacg gggcggagac ggggcgcgag
    14221 aagcagcacg cgcagatgat cgtggacctg ctgcattcgt ccgccaaccg gttcgccatg
    14281 gccggcgcgc gcatccacga cttcacccac ccgcactgga agaaggacgt cgacatcctc
    14341 gtgggcggcg ccgggcaggt catcgcctac gtgacgatcc cgaaatgcac ctcggtggtc
    14401 caggccgagg agatgatcct ctacgtgcag tcggtggcga ggcgcaggaa gatcaagcgg
    14461 gcgattcccg tgcacgtgct catcgagacg cacggcgcgc tgcacgacgc gtgggagatc
    14521 gcgcaactgc ccggcgtgca ggtgctcgac ttcgggctca tggatttcgt gagcggccac
    14581 cacggcgcca tcaacgcctc caacatgcgc acgccggggc agttcgagca cccgctggtc
    14641 gcgcgcgcaa aggtcgatgt cgtggccgcg gcgctggcca acggggtggt cccgtcgcac
    14701 aacgtctcgc tggacctcaa gaacagctac aacgtcttcc gcgatgcgtg gcgcgcccgc
    14761 agcgacttcg gcttcctgcg catgtggagc atccacccct cgcaggtgca gcccatcgtc
    14821 gacgcgatga agccggacct gaacgaggtg gcgaccgccg gagcgatcct tctcgaggcc
    14881 cagaaagcca gttggggtcc gatccagttc gagggcgagc tgcacgaccg cgccacctac
    14941 cgctatttct gggaactgct ccagaaggcg cgcatcaccg gcgtgaagat gccggaagac
    15001 gctgcgcggg ccttcttcga ttgacggggc aggttgcgcg taccggaaag cgacgaggat
    15061 tcgattcacg ccgattcaac cgaagggcaa cctgacccgg agagaagaag aaagatgacc
    15121 accatgaccc ccggcgcgaa attccgcgcc gcgctgaagg acgaaaagcc cctgcaggtg
    15181 ccaggcgcca tctgcgccta ccacgcgacg ctcgccaagg cctcgggatt cagggcgatc
    15241 tacctttccg gcggcggcgt ggccgcgggc tccctgggca ttcccgacct ggggatcacc
    15301 aacctggacg acgtcctcac ggacatccgc cgcatcaccg acgtgtgcga cctgccgctg
    15361 ctggtggacg tcgacaccgg gttcggtgcg agcgccttca acgtcgcgcg cacgacgaag
    15421 tcgctcatca agttcggcgc ggc
//
LOCUS       DASUEV010000113         7214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1400810, whole genome shotgun sequence.
ACCESSION   DASUEV010000113 DASUEV010000000
VERSION     DASUEV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400810"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VFV55_03735"
     CDS             complement(<1..1071)
                     /locus_tag="VFV55_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX4943438.1"
                     /translation="MNSPSTSIATASSPTPFKLTRQFSVVALACVVAVAAALGFVVSR
                     LLGDELLKRDAVVTMEFVQSIVSTDETAPHFRSAIQLPIHEALEDTFRHFAKMPDVLR
                     ANVYTRDRRAIWSSDPKIIGRQFGENEELDNALAGNPVFKSGFVSKEEHVEASELVAK
                     DSFNQFVEIYVPVRDFAGSVVGVVELYKTPNALFEAIRDGKRTIAIVSTAGGILLYLV
                     LIGIVHRADRIMHDQQRRLLEGERLAAVGEMAATVAHAIRNPLATIRTSVEVALDQDP
                     GNFGEPGEDIIAEVDKIEDWIRQILAFSRPGSMKVENLALNDLVRKSLGTCERESNRL
                     GVRVETRLAEPSPPARGDAALVE"
     gene            complement(1166..1969)
                     /locus_tag="VFV55_03740"
     CDS             complement(1166..1969)
                     /locus_tag="VFV55_03740"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX4943439.1"
                     /translation="MPVTISQLNIYPVKGLKGIAVESAMATGRGLAHDRRFMVVDQDR
                     RFLSQRELPVMATVWTEIAGSELRLAAPECGEVAVPVAPVDGEPLDVGIWSSTCRAIA
                     PSREADRWLASFLARSCRLVYMPEETERPVDPRYAGPGHCVGFADAFPVLVAGEASLA
                     DLNARLPRPVPMDRFRANVVLSGAGAFAEDGWLELRAGEVRLRLAKPCGRCQVTTTDQ
                     ATGEVHSPEPLATLSAYRPSKEFGARFGMNAVVLHTGLLQTGMEVATTI"
     gene            2065..3183
                     /locus_tag="VFV55_03745"
     CDS             2065..3183
                     /locus_tag="VFV55_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4943440.1"
                     /translation="MALWDKLMGELVDIVEWLDDSNNTLAYRFERYQNEIKYGAKLVV
                     REGQVAVFINEGKLADVFRPGTHELTTQNLPVLATLKGWKYGFNSPFKAEVYFCSTRK
                     FTDLKWGTPGPAMMRDKDFGAVRATAFGIYAIRVKDAGIFVKDLVGTDNRFTTDEIQE
                     NLRGKIGLRIKEVMPELGIPVLDLEPKVTELGKRLCDRLAPEFEGLGLELVEVQVQDI
                     GLPEEVEKAIDKRGAMAAIGNLQDYTRYETASAIRDAANNPGGAAGAAVGIGAGVAMG
                     AQMINAMSGNAATAPLGAGAAVAPPIPGAVSFLVAIGGQQSGPFDLATLQQQVAGGQL
                     NRASLVWKAGMAQWAKAGEVQELAPLFANVPPPIPPSA"
     gene            3187..4311
                     /locus_tag="VFV55_03750"
     CDS             3187..4311
                     /locus_tag="VFV55_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943441.1"
                     /translation="MEQADLQQTRPESAFVKTFPCTGCGAKLSFAPGTRSLQCEYCGT
                     ANAIAENDARIEELDFDAYLKALEGNMEVQEVEEVKCDKCGAAQSMVGNLFAGHCTFC
                     GAAIVSKSYASRRIKPKAIVPFQVKRERAAESFRRWVRGLWLAPGDLKKYAQSDAGLT
                     GVYLPYWTYDCQTASDYSGERGDDYYTTQSYTTQDSSGRSVTQTRRVKHTRWTPASGH
                     VERFHDDVLVMATRTLPANITDSVTRFDLKGLVPYQPEFVSGFQAEAYQVTLKDGYPI
                     ARRSIDEAVQALVRRDIGGDQQRIASIATRYSEVKFKHVLLPVWISAYRYRDKVYRFL
                     INGQTGEVSGESPKSWWKIAFLVAGILVVLFVILMAGGKG"
     gene            complement(4434..4940)
                     /locus_tag="VFV55_03755"
     CDS             complement(4434..4940)
                     /locus_tag="VFV55_03755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4943442.1"
                     /translation="MTIKVGDRLPAGTVQEFIEVEGNGCSLGPNTFKVEDLVKGKKIA
                     IFGLPGAFTPTCSAKHVPSYLNNYAALKAKGVDEIWCFAVNDPFVMGAWSRDQKAGGK
                     IRFMADGSAAYSKQLGLELDLVARGMGMRCQRFSMFVDNGVVKTLNVEGPGKFEVSDG
                     ETMLKQVA"
     gene            complement(4981..5622)
                     /locus_tag="VFV55_03760"
     CDS             complement(4981..5622)
                     /locus_tag="VFV55_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX4943443.1"
                     /translation="MDFPVWITYLAATIILSVSPGPGVFSSLAAGLNHGFRRGMWNAV
                     GMQVANFALLAFVSVGLGAILLASESLFTTVKWGGVAYLVWLGFVTWRSEPHHFDSRA
                     DAPQGKDSREVFLKGFLVNLTNPKGIIFYVAVLPQFIDLARPQFVQYAILGVTTLVVD
                     LVVMAGYIAVAARVLAVMEDPSHLRWVNRILGGAFVAAGVALAAFRRGHAAAS"
     gene            complement(5622..6416)
                     /locus_tag="VFV55_03765"
     CDS             complement(5622..6416)
                     /locus_tag="VFV55_03765"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4943444.1"
                     /translation="MKDNVVIITGASKGIGAELARRMAAKGAMLALVARDRPALEKVA
                     AECAALGARSEVIVADVAVGEDCRRMVEHVIAAFGRIDTLVNNAGATMWARFEDIDDM
                     AILARIMQVNYMGAVYCTHYALPHLKAARGRIVGIASLTALVGVPTRSGYAASKHAMR
                     GFFDSLRIELADTGVTVTMIYPGFVSTGIRENATGPDGKPIQVSPVKEGEVMTVEECA
                     RITLGAIAARKREVVMTVRGKMGILLKLVAPGLVDRIARRAIETGR"
     gene            complement(6506..>7214)
                     /locus_tag="VFV55_03770"
     CDS             complement(6506..>7214)
                     /locus_tag="VFV55_03770"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX4943445.1"
                     /translation="ELPQRLFGLQLRDLSGIGRQMEKRLERHGIRTVEALCSRTREEL
                     CAVWGGVGGEVMHDRLRGEAVALRDSDTHSISHSSVLAPDKRNAADAFAILDRLVQKA
                     AMRLRKSEHYASRLAVAIKYLDGSRWDAGMRLVDTQDTVTFLHALEKLWAGRPQDRRT
                     ILQVGMAFSDLVTEAGHTGSLFAAESRSKSLYEALDKLNARFGKQAVYFASAHKARDR
                     GGMHIAFNHIPDPETER"
BASE COUNT         1236 a         2384 c         2392 g         1202 t
ORIGIN      
        1 ctcgacgagg gcggcgtcgc cgcgcgcggg cggtgacggc tccgccaggc gggtctcgac
       61 gcggacgccg aggcggttgc tttcccgctc gcaagtgccg aggctcttgc ggacgaggtc
      121 gttgagggcc aggttctcga ctttcatgct gccgggccgc gagaaggcga ggatctggcg
      181 gatccagtcc tcgatcttgt cgacttcggc aatgatgtcc tcgccaggct ccccgaaatt
      241 gcccgggtcc tggtcgagcg ccacctccac cgaggttcgt atcgttgcca aagggttgcg
      301 gatggcatgc gccacggtcg cggccatttc gcccacggcg gcgagccgct cgccttcgag
      361 cagccggcgc tgctggtcgt gcatgatccg gtcggcgcgg tggacgatgc cgatgagcac
      421 caggtagagc aggatcccgc ctgccgtcga gacgatggcg atggtgcgct tgccgtcccg
      481 gatcgcctcg aagagggcgt tcggcgtctt gtacagctcg accacgccca cgacgctccc
      541 ggcgaaatcg cgcaccggca cgtagatctc gacgaactgg ttgaagctgt ccttggcaac
      601 gagctccgag gcttccacgt gttcttcctt gctcacgaag ccgctcttga acacgggatt
      661 gccggcgagc gcgttgtcga gctcctcgtt ctcgccgaac tgcctgccga tgatcttcgg
      721 gtcgctggac cagatggcgc ggcgatcgcg cgtgtagacg ttggcgcgca acacgtccgg
      781 catcttcgcg aagtgccgga acgtatcctc gagcgcctcg tgtatcggca actggatcgc
      841 cgaacggaag tggggggcgg tctcgtcggt gctcacgatg ctctgcacga attccatcgt
      901 cacgacggca tcgcgcttca ggagctcgtc gcccagcagg cgggaaacga cgaaaccgag
      961 cgccgcggcg accgcgacga cgcaggccag ggccacgacg gaaaactggc gcgtgagctt
     1021 gaacggtgtc ggagacgaag ccgtggcgat gctggtggaa ggcgagttca tgggtgcgca
     1081 gggtttgtca ttgtgccggc agctgctgaa tggatggtgg catctccatg gtttccgatc
     1141 aaggcgggtg aagtcgcaag cccgttcaaa tggtcgtagc aacctccatg cctgtttgca
     1201 gcaggcccgt atgcagcacc acggcgttca tgccgaatcg cgcgccgaac tccttgctcg
     1261 gacggtatgc ggacaaggtc gcaagcggtt ccggtgaatg gacttccccg gtggcctgat
     1321 cggtggtcgt gacctggcaa cgcccgcagg gcttcgccag gcgcaggcgg acctcgcccg
     1381 cgcgcaattc cagccagccg tcctcggcaa aggccccggc gccggaaagc acgacgttcg
     1441 cccggaaccg gtccatcggg acgggcctgg gcagccgggc gttcaggtcg gccagcgagg
     1501 cctctcccgc gaccaggacg gggaaagcgt cggcaaaacc aacgcaatgg cccggcccgg
     1561 catatcgtgg gtcgacgggg cgctcggtct cctcgggcat gtagacgagc cggcaggaac
     1621 gggcgaggaa cgacgcgagc catcggtccg cctcccggga gggcgcgatg gcccggcacg
     1681 tgctgctcca gatcccgacg tccaggggct cgccgtcaac cggcgccacc ggcacggcca
     1741 cctcgccgca ctccggcgcg gcgaggcgca gttcgctgcc cgcgatttcc gtccacaccg
     1801 tggccatgac cggcaactcg cgctgcgaca gaaagcgccg gtcctggtcg accaccatga
     1861 agcggcgatc gtgcgcgagg ccgcggccgg tggccatggc ggattcgacc gcgatcccct
     1921 tgaggccctt caccgggtag atattcagct gcgagatggt cacgggcatg gggcgaggat
     1981 tgaatcgggg ggatgatcgg gggtacttta ccggttccgg ccgggggaat tccggcaggc
     2041 ttccattcct gaaaaggcga cgccatggct ctgtgggaca agttgatggg cgaactggtc
     2101 gacatcgtcg agtggctcga cgattcgaac aacacgctcg cgtaccgctt cgaacgctac
     2161 cagaacgaga tcaagtacgg cgcgaagctc gtggtccgcg agggccaggt ggcggtgttc
     2221 atcaatgaag gcaagctcgc cgacgtgttc aggcccggca cccacgagct caccacgcag
     2281 aacctgccgg tccttgcgac gctcaagggc tggaagtacg gattcaacag cccgttcaag
     2341 gcggaggtgt acttctgttc cacgcgcaag ttcactgacc tcaagtgggg taccccgggc
     2401 ccggcgatga tgcgcgacaa ggatttcggc gccgtgcgcg ccacggcctt cggcatctat
     2461 gccatccggg tgaaggacgc cggcatcttc gtgaaggacc tggtcggcac cgacaaccgc
     2521 ttcaccaccg acgagatcca ggagaacctg cgcgggaaga tcgggctgcg catcaaggag
     2581 gtgatgccgg agctgggcat ccccgtgctc gacctcgagc ccaaggtcac cgagctgggc
     2641 aagcggcttt gcgaccggct cgctcccgag ttcgagggcc tgggcctgga actggtcgaa
     2701 gtccaggtgc aggacatcgg cttgccggag gaggtcgaga aggccatcga caagcgcggc
     2761 gccatggcgg ccatcggcaa tctccaggac tacacccggt acgagaccgc ttcagccatc
     2821 cgcgacgccg ccaacaaccc gggtggcgcg gcgggtgcgg ccgtgggcat cggcgcgggt
     2881 gtcgcgatgg gcgcacagat gatcaacgcg atgtcgggaa atgcggcaac tgcgccgctt
     2941 ggggccggcg ccgccgtagc tccgccgatt cccggcgcgg tttcgttcct cgtcgccatc
     3001 ggtggccagc agtcgggccc gttcgacctg gcgaccctcc agcagcaggt ggcgggcggc
     3061 cagctgaacc gcgcctcgct cgtgtggaag gccggcatgg cgcagtgggc caaggcgggc
     3121 gaggtgcagg aactggcgcc cttgttcgcc aacgtgccgc ccccgattcc cccttccgcc
     3181 taggcgatgg aacaggcgga cctccagcag acccgacccg agagcgcctt cgtcaagaca
     3241 tttccctgca ccggttgcgg ggcgaagctc tcgttcgcgc cgggaacgcg cagcctgcag
     3301 tgcgagtact gcggcaccgc gaacgcgatt gccgagaacg acgcgcgcat cgaggaactc
     3361 gatttcgacg cctacctgaa ggccctcgag ggcaacatgg aggtgcagga agtcgaggag
     3421 gtgaagtgcg acaagtgcgg cgccgcgcag tcgatggtcg gcaacctctt cgcggggcac
     3481 tgcaccttct gtggcgccgc gatcgtctcg aaaagttatg cgagccggcg catcaagccc
     3541 aaggcgatcg tgccgttcca ggtgaagcgg gagcgcgcgg cggaaagctt ccggcgctgg
     3601 gtgaggggcc tctggctcgc cccgggcgac ctgaagaaat atgcgcagag cgacgcgggc
     3661 ctcaccggcg tgtacctgcc gtactggacg tacgactgcc agactgcgag cgactattcc
     3721 ggcgagcggg gcgacgacta ttacacgacg cagtcctaca cgacgcagga ctcgtcgggc
     3781 cgttcggtca cgcagacccg gcgcgtgaag cacacgcgct ggacgccggc ctcgggacat
     3841 gtcgagcgct tccatgacga cgtgctcgtg atggccacgc ggaccctccc ggccaatatc
     3901 accgactcgg tcacccgctt cgacctgaaa gggctggtgc cctaccagcc cgagttcgtg
     3961 agcggcttcc aggccgaggc ctaccaggtg accctcaagg acggctaccc gatcgccagg
     4021 cggtccatcg acgaggcggt gcaggccctc gtccggcgcg acatcggcgg cgaccagcag
     4081 cgcatcgcct cgatcgcgac gcgctactcg gaggtgaagt tcaagcacgt gctgctgccg
     4141 gtatggatct cggcctaccg ctaccgcgac aaggtctacc gcttcctcat caacggccag
     4201 acgggcgagg tgtcgggcga gagcccgaag tcgtggtgga agatcgcctt cctcgtcgcc
     4261 gggatcctcg tcgtgttgtt cgtgatcctg atggcgggcg ggaaggggta gggagagggg
     4321 cccgattaaa ggggtcaggt tactttgatc aaaggggtca ggttacttta ctgtcgacgg
     4381 atttcgatcg ggctgtaaag taacctgacc cctttaatcc acttcctctc gcttcaggcg
     4441 acttgcttga gcatcgtttc gccgtcggaa acctcgaact tgccggggcc ttcgacgttg
     4501 agcgttttca ccacgccatt gtcgacgaac atcgagaaac gctggcagcg cattcccatg
     4561 ccgcgcgcga cgaggtcgag ttccaggccg agctgtttgg agtaggcggc gctgccgtcg
     4621 gccatgaagc ggatcttgcc gccggctttc tggtcgcggg accacgcgcc catgacgaac
     4681 gggtcgttca ccgcgaagca ccagatctcg tccacgccct tcgccttgag tgccgcgtaa
     4741 ttgttgaggt agctcggcac gtgcttggcc gagcaggtgg gggtgaacgc gcccggcagg
     4801 ccgaagatcg cgatcttctt gcccttcacg aggtcctcga ccttgaaggt attcggcccg
     4861 agcgaacagc cgttgccctc gacttcgatg aattcctgga ccgtgcccgc gggcagccgg
     4921 tcgccgactt tgatggtcat cctcgatgct cctctcgttg ttgggttgtt gcggaaagaa
     4981 tcacgacgcc gcggcatggc cgcggcggaa ggccgccagc gccacgcccg ctgccacgaa
     5041 cgcgccgccg agaatgcggt tcacccagcg caggtgcgac ggatcctcca tcacggcgag
     5101 cacgcgcgcg gcaaccgcga tgtaacccgc catcaccacg agatccacga cgagcgtggt
     5161 caccccgagg atggcgtact gcacgaattg cggccgcgcc aggtcgatga actgcggaag
     5221 cacggccacg tagaagatga tccccttcgg attggtgagg ttgacgagga agcccttcag
     5281 gaatacctcg cgcgaatcct ttccctgcgg cgcgtcggcg cgcgagtcga aatggtgcgg
     5341 ttcggagcgc caggtgacga aacccagcca cacgaggtag gcgacgccgc cccatttgac
     5401 ggtggtgaac agcgattccg aggcgaggag gatcgcgccc aggcccacgg atacgaaggc
     5461 cagcagcgcg aaattggcca cctgcatgcc caccgcgttc cacatgccgc ggcgaaagcc
     5521 gtggttcagg cccgcggcga ggctggaaaa cacgcccggt cccggcgaga cggacaggat
     5581 gatggtggcg gcgagatagg tgatccagac cgggaaatcc atcagcgacc cgtttcgatc
     5641 gcgcgccggg cgatgcggtc caccagcccg ggagcgacga gtttgagaag aattcccatt
     5701 ttaccgcgaa ccgtcatgac cacctcgcgc ttcctcgccg cgatcgcccc cagggtgatc
     5761 cgggcgcatt cctccacggt catcacctcg ccttccttca cggggctcac ctggatgggc
     5821 ttgccgtcgg gccccgtggc gttctcgcgg atgccggtgg agacgaagcc ggggtagatc
     5881 atcgtgacgg tcacgcccgt gtccgcgagc tcgatccgca gcgagtcgaa gaagccgcgc
     5941 atggcatgct tggatgcggc gtagcccgag cgcgtcggaa ccccgacgag ggccgtgagg
     6001 ctcgcgattc cgacgatccg gccgcgcgcg gccttgaggt gcggcagcgc gtagtgggtg
     6061 cagtagacgg ctcccatgta gttcacctgc atgatgcgcg cgaggatcgc catgtcgtcg
     6121 atgtcctcga atcgcgccca catcgtggcc cccgcgttgt tcaccagcgt gtctatgcgg
     6181 ccgaacgcgg cgatcacatg ctccaccatc ctgcggcagt cctccccgac cgccacgtcg
     6241 gcgacgatca cctccgagcg cgcgcccagt gccgcgcact cggcagccac cttctccagc
     6301 gccgggcggt cgcgcgccac gagcgccagc attgccccct tcgccgccat ccgcctggcc
     6361 agttcagcgc cgatgccctt cgacgcgccg gtgatgatca ccacgttgtc cttcacgccg
     6421 tcctctccat gtgcgccatc gcattactca tcgacgatca tccgtgttca tccgtggttc
     6481 caacgccttt cccccgcatc actcgctacc gttccgtttc cggatcggga atgtggttga
     6541 aggcgatatg catcccgccc cggtcccgtg ccttgtgcgc gctcgcgaaa tacaccgcct
     6601 gcttcccgaa ccgcgcattg agcttgtcca gcgcctcgta gaggctcttc gacctcgatt
     6661 ccgccgcgaa gagcgagccg gtgtgccccg cctccgtcac gaggtcggaa aaggccatgc
     6721 ccacctggag gatcgtgcgc cggtcctgcg gcctcccggc ccacagcttc tccagcgcgt
     6781 gcaggaaggt gacggtgtcc tgcgtgtcca cgaggcgcat gcccgcgtcc cagcgcgagc
     6841 cgtcgagata cttgatcgcc accgccaggc gggaagcgta gtgttccgac ttgcgcagcc
     6901 gcatcgccgc cttctggacc aggcgatcga ggatcgcaaa ggcgtccgcc gcattgcgct
     6961 tgtcgggcgc cagcacgctc gaatgcgaga tcgagtgcgt gtcggagtca cgcaaggcca
     7021 ccgcctcgcc gcgcaggcga tcgtgcatga cctcgccgcc cacgccgccc cacaccgcgc
     7081 aaagctcttc acgggtgcgc gagcaaagcg cttccacggt ccggatgccg tggcgctcca
     7141 ggcgcttttc catctgcctg ccgatgccgg aaaggtcgcg caactgcagc ccgaagagcc
     7201 gctgcggcaa ctcg
//
LOCUS       DASUEV010000114        11024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_720406, whole genome shotgun sequence.
ACCESSION   DASUEV010000114 DASUEV010000000
VERSION     DASUEV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720406"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFV55_03775"
     CDS             complement(<1..543)
                     /locus_tag="VFV55_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943446.1"
                     /translation="MKTLLRIVATLASLLALQSTGADMDMAVAQKWTSAKVVKYRVDG
                     VHNGREAVVFGDYEGKADVIDRLTVEFSWDVRKSAVIEPVTVKDGKSELGNIKSDGTN
                     CPPPKLKGEYEHFQIVSTSMSSRDQIQIKGTRTYPPASVSNYPGSCSMRAIPGGKEEA
                     LLWVTGASPEVLGMPIVPGGP"
     gene            complement(596..1099)
                     /locus_tag="VFV55_03780"
     CDS             complement(596..1099)
                     /locus_tag="VFV55_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943447.1"
                     /translation="MSRRGFVSSRAFSRVILLSFAILGGCYESSTPLGPAERGVVNPE
                     FLGTWTCRNAADDANAKTLLVVAFDSSRYFLEWRDKEETTRWAAHSSIVGGALLHNVR
                     ELKADGRDTKWVFLRALAPTESELLLWLVKDDSLKELGEPQALREIRRRVKDESLYKP
                     WATCMRA"
     gene            complement(1126..1455)
                     /locus_tag="VFV55_03785"
     CDS             complement(1126..1455)
                     /locus_tag="VFV55_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEX4943448.1"
                     /translation="MLLLIGSGVIAAVSIPLILGFVPPNRIYGFRTRQTLSNEALWYQ
                     ANFFAGWAFLIAAFASVVLLALVRGGAFPGVASEVAAFALPVVVAIVACFVYLRRITD
                     GVAGGRR"
     gene            complement(1632..2687)
                     /locus_tag="VFV55_03790"
     CDS             complement(1632..2687)
                     /locus_tag="VFV55_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEX4943449.1"
                     /translation="MRKPLSFLALLAIAAAALAADYAPVRPGLELAFPADFGAHPEHR
                     IEWWYATGQLDTARGPIGFQVTFFRLRNKEADANPSRFAPRQLLFAHAALADPAHGRL
                     RHDQRIARAFPGIAEARPGDTHVFIDDWSFKRVDGRYLARIPGEGFALDLVLAPTQPL
                     LPQGEAGYSRKGPSPAQASGYYSEPQLAVTGTVKIDGRAIDAKGVAWLDHEWSSEILA
                     GNAVGWDWVGLNLEGGGALMAFRLRDKAGGTVWAGGTLRADGKVRAFSDDEVGFTPRR
                     TWRSPRTGVTWPVQMDFEVAGQLWSLAPLMDDQEMDARGSTGTLYWEGAVTATGPNGA
                     KGRGYLELTGYSERLRF"
     gene            complement(2680..5214)
                     /locus_tag="VFV55_03795"
     CDS             complement(2680..5214)
                     /locus_tag="VFV55_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX4943450.1"
                     /translation="MNAALVATLLRGSLSGNRWRSLLAIACIGLGVALAGAVHTLHAS
                     ALDEIDRAARLLSGTADEQVRGPRNGFDDATYAAVALHPAVAVASPVVELEATAAGLE
                     APAAGRETTIRVLGIDPFRASRLQPGFLAEGATSRAGGTPTLLDAHAAWLTPAAIAKA
                     GATVGGDVALRGPLGEVRLKIAGTLPALEGGGPIAVMDIAAAQLAFGRVGVIHRIDLR
                     LAPGAARETVLREIAAKLPPGVSIVRPDESTMRTAGLTRAYRVNLTALALMALFTGGF
                     LVFSTLALQAAKRRQEFALLRALGLTGNGVRAFLALEGGLLGLAGAALGTVLGLVASR
                     AMLERFGYDLGAGFFPGSGDTFAPDAVALAVIALLGVFTAAAAALAVTRSLARLDVAQ
                     ALKDRHLDLPSPPGGWAWPIALALAAILAAWLPPAGEIPVGGYLSIGLALAAAVMAVS
                     PASRLLLDRIPASDAPLRTLARAQVKSLPGHLSAMVSGIVVSVALTAAMAIMVFSFRV
                     SLDQWLTGILEADFFARAAPQGDAVVFGPEAQAQIAAVPGVARIDPIRFERLAPEGST
                     GLLTVMARPVDAALLAGFQVDPPAVPARDGAIPAWISEASVDLFGWRVGQVVTIPLAG
                     RSASFRVAGIFRDYARTWGALLVDLADYRALTNDAFANDIAIHLASGADAGAVEARIA
                     KVVAQVPGAELHDASYIHARALRIFDRTFAATYALEAAAILIALAGVTSSFAALAWSR
                     RREFGVLRHLGLARGDVMRLLALEGGAAGIIGALLGLVAGAAVSVVLVHVVNRQSFHW
                     GMAIHWPWLGLAVLLGSVVFLCALGASLAGRAAVAREAVHAVKDDA"
     gene            complement(5211..5921)
                     /locus_tag="VFV55_03800"
     CDS             complement(5211..5921)
                     /locus_tag="VFV55_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4943451.1"
                     /translation="MLEVRGLAKSWPGGAPLFDSVDLSVAPGELVAILGESGAGKSTL
                     LNIVAGLDRADAGTVTVSDSTRSQTPPGVRLRVESGTVVTALTDDELALWRRRNVGFV
                     FQAFHLLPYLTVGENVGLPLLLNGVPGAERRSRSIAALESVGMSGFEDRRPYSLSGGE
                     MQRVAVARAVAHRPSLVLADEPTGNLDENNAASALQALRSAVKREGAAGLLVTHSAVA
                     AACADRVLRLAGRRLGPA"
     gene            complement(5936..6502)
                     /locus_tag="VFV55_03805"
     CDS             complement(5936..6502)
                     /locus_tag="VFV55_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX4943452.1"
                     /translation="MSEHGIHVHGPHDHEVEHQAQHGGMGQRIAIFTAVLATIGAVVS
                     FLGGHTQNEALLYKNDAVLKRAEASDQWNYYQAKSMKQNLAEFAAAMSADPKKSEFYT
                     VEAARYAKEKKDIEKEARRLEDDSKASNRKSEKALHPHEMLAISMTLLQIAIALASIT
                     VLTKKDWLLWGAGGSALAGVALGVIAWF"
     gene            complement(6539..6970)
                     /locus_tag="VFV55_03810"
     CDS             complement(6539..6970)
                     /locus_tag="VFV55_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4943453.1"
                     /translation="MKNVSELLKGKPTRMVSVKPEDTVLDAIKVMARENIGAAIVMSG
                     DRLCGILSERDYARKVILRGRSSETTLVEEIMTPNVVCVSPRAKSRDCMGLMSERNIR
                     HLPVVDEGRVIGMVSIRDIVTDIIADQDFTISQLESYISGQ"
     gene            complement(7023..8663)
                     /locus_tag="VFV55_03815"
     CDS             complement(7023..8663)
                     /locus_tag="VFV55_03815"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX4943454.1"
                     /translation="MTRESMEFDVVVVGGGPAGLATAIHLRQLAAARDAEVSVCLIEK
                     GSEIGAHILSGAVIDPKALAELFPDWKEKGAPLKQPVTEDRFLFFSETGTTQVPNWLL
                     PACFGNFGYYAASLGNLCRWLGAQAEALGVEIYPGFAAAEVLFDEEGRVMGVATGDVG
                     IGKNGERTTRYQPGMELHAKYTVFAEGCRGNLGKGLQERFRLREGRDPAVYGLGLKEL
                     WEIDPAKHRPGLVIHGAGWPLDNSTYGGSWMYHLEDNQVSVGLVVGLGYTNPYLSPFE
                     EFQRFKTHPAMRAFLEGGRRVAYGARAINAGGLQALPRLVFPGGVLVGDDAGFLNAAK
                     VKGSHAAIKSGMLAADAAFDALAAGRSRDELTAFPEAFEKSWLHDELYRARNFKPFMT
                     KGLVAGSLLVGIDQVVFGGKAPWTLHHPGPDHEQLRPKGECVPPVYPKPDGVLTFDRL
                     SSVFVSNVAHEEDQPAHLTLKDAAVPIAVNLALYDAPETRYCPAGVYEIVTEGQNSRL
                     QINAQNCVHCKTCDIKDPTQNIHWVVPEGGGGPNYPNM"
     gene            8782..10785
                     /locus_tag="VFV55_03820"
     CDS             8782..10785
                     /locus_tag="VFV55_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX4943455.1"
                     /translation="MGPWAISLLMITGFAGFAVLAWRKLAIVVALQPEVRWDRPGERI
                     ARLLTMGFGQSRMVAGEWKPGIMHAAIFLGFMALLVRKLNLIAIGYDPLALIPGWPGA
                     VYAAIKELVEVGVLAALVYAFWRRFVLKPKRLEPNREAILILSLIAVIVVTDFLFDGF
                     RFVKYAADAGITHERGFAPVGAALAVAFAGLSPGAIDLGLHASYWVQMVTVFTFLVLL
                     PAGEHFHIVTALPAVFLARATPLNRVPSVDLERIMADDAGDDMKVGAKTAADLLWKEA
                     FDPFTCTECGRCKDSCPTFLTGKPLAMKWVNDSLKHHLMEKREPIVAGKEADLPPLVG
                     GVISEETLWACTTCGYCERSCPIGLEHLGRFYRLRQHQVMMEGAFPQELKAVFDAYES
                     QSNPWGLPADTRGDWAKDLGVPLLENAQQAREIDWLFYVGSAQSFDLRAQKTAKAFVK
                     ILKAAGLRFAILGPREGSTGECVRRAGNEMLFQSLAQALVETLNGLGVARIVTCDPHA
                     FNSLAHEYPEFGGKYEVIHHTQLIDRLIRDGKIAPRRVFEGVIYHEPCYLARHNGEYE
                     APRRVLAAVSRDTPPEFVMARDKAMCCGAGGGRMWMEEHTGKRINVLRAEQALERAPK
                     AIVTACPYCAIMLRDGVAQLGHADDLPTRDIAELVAEAIEPAT"
BASE COUNT         1796 a         3763 c         3755 g         1710 t
ORIGIN      
        1 ggggccgccc ggcacaatcg gcatgccgag gacttccggt gaagctcccg tcacccacag
       61 gagcgcctct tctttgccac cgggaatggc gcgcatggag cagctccccg ggtagttgga
      121 gacgctcgcc ggcggatagg tccgcgtgcc cttgatctgg atctggtcgc gggaactcat
      181 cgaagtcgac acgatctgga agtgctcgta ctcgcccttg agcttcggtg gcgggcagtt
      241 ggtgccgtcg gacttgatgt tgccgagctc cgactttccg tccttcaccg tgaccggctc
      301 gatgaccgcg ctcttcctga cgtcccacga gaactcgacc gtgaggcggt cgatcacgtc
      361 cgccttgccc tcgtaatcac cgaatacgac ggcctcgcgc ccgttgtgca cgccgtccac
      421 gcgatacttg acgaccttgg ccgaagtcca tttctgcgcc acggccatgt ccatgtccgc
      481 gccggtcgat tgcagggcca gcaggctggc gagagtggcg acgatccgaa gcagggtttt
      541 catggcaggg caccttggca cggcctcggc gtgcgagtca agcgaacagg cccgcctacg
      601 cccgcataca cgttgcccac ggcttgtaca gcgactcgtc cttcacgcgc cgcctgatct
      661 cgcgaagggc ctgcggctcg cccagctcct tcaggctgtc gtccttcacc agccaaagca
      721 gcagctcgga ctccgtgggt gccaatgcgc gcaggaagac ccacttcgtg tcgcgaccgt
      781 cggccttcag ttccctcaca ttgtggagca gcgcgcctcc gacgatcgag gagtgcgccg
      841 cccaccgggt ggtctcctcc ttgtcgcgcc attccaggaa gtagcgggaa gaatcgaacg
      901 ccacgaccag cagcgtcttc gcgttcgcat cgtcggctgc attccggcag gtccaggttc
      961 cgaggaactc gggattgacc acgccgcgct cggcaggccc aagtggcgtg gacgactcgt
     1021 aacatccgcc caggattgcg aaagagagca ggatgacacg cgagaaggca cgcgagctca
     1081 cgaagccacg acggctcacg gaccgctcgg acgcgttgag cgcggtcacc tgcgtccgcc
     1141 agccaccccg tcggtgattc tccgcagata gacgaagcac gccacgatcg cgacgaccac
     1201 cggcaatgca aaggccgcga cctcggaagc cactcccgga aacgcaccgc cgcggacaag
     1261 agcaagcagg acgacgctcg cgaatgccgc gatcaagaat gcccaacccg cgaagaaatt
     1321 ggcctggtac cacaacgcct cgttcgagag ggtctggcgc gtccggaagc cgtaaatgcg
     1381 attgggcgga acgaacccga ggatgagcgg aatgctcacc gcagcgatga ctccggaccc
     1441 gatcagcaac agcatgctcg aactcattcc gtccttcgac cgacggccaa cagcaaacgc
     1501 gctggcacgt gccattcggc gcaaatgcag gtgttaatcg ggagtttgtc caagtatgac
     1561 gccgtggtca acccggacgc gatgacagga aaggtgcggc ggctcgagcg cgaaggggac
     1621 ggtgatgtcg ctcaaaagcg cagcctttcc gaatagccgg tgagctcgag gtagccgcgg
     1681 ccctttgccc cgttcggccc cgtggcggtg accgcgccct cccagtagag cgttccggtg
     1741 gagccgcgcg cgtccatctc ctggtcgtcc atcagcgggg cgaggctcca tagctggccc
     1801 gccacctcga agtccatctg cacgggccag gtgacgccgg tacgcggtga tcgccaggtg
     1861 cggcgcggcg tgaatcccac ctcgtcatcg gaaaacgcgc ggaccttgcc gtcggcccgc
     1921 agcgtgcccc cggcccacac cgtgccgccc gccttgtcgc gcagccggaa cgccatgagc
     1981 gcgccgccgc cctcgaggtt cagccccacc cagtcccagc ccacggcgtt cccggcgagg
     2041 atctcgctgg accactcgtg gtcgagccat gcaacaccct ttgcgtcgat cgcccggccg
     2101 tcgatcttca ccgtgcctgt caccgcgagc tgtggctcgc tgtaatagcc gctggcttgc
     2161 gcggggctcg gtcccttgcg gctgtagccc gcctcgccct gcggcaggag cggctgcgta
     2221 ggggccagaa cgaggtcgag cgcgaaccct tcacccggaa tgcgcgcgag gtagcgcccg
     2281 tccacgcgct tgaacgacca gtcgtcgatg aagacgtggg tgtcgcccgg ccgcgcttcc
     2341 gcgatgccgg gaaaggctcg cgcgatgcgc tggtcgtggc gcagccggcc gtgcgccgga
     2401 tcggccagtg cggcgtgggc gaagagcaac tgccgcggcg cgaagcggct ggggttcgcg
     2461 tcggcctcct tgttgcgcag gcgaaagaag gtgacctgga agccgatcgg cccgcgcgcc
     2521 gtgtccagtt gccctgtcgc gtaccaccac tcgatgcggt gctccgggtg ggcgccgaaa
     2581 tcggcgggga aggcgagctc gaggcccggc cgaacgggtg cgtagtccgc agccagtgcc
     2641 gccgcggcga tggcgagcaa ggcgaggaag gacagtggct tacgcatcgt ctttcaccgc
     2701 gtgcaccgcc tcgcgagcca ccgccgcccg ccccgcgagc gaagcgccca gcgcgcacag
     2761 gaacaccacg ctgccgagca ggacggccag cccgagccac ggccagtgaa tcgccattcc
     2821 ccagtggaac gactggcggt tcaccacgtg caccagcacc acgctcacgg ccgcgcctgc
     2881 gacgaggccg agcaaggccc cgatgattcc cgcggcgccc ccttcgaggg ccagaagccg
     2941 catcacgtcg ccgcgcgcga gccccaggtg gcgaagcacg ccgaactcgc gccgccgtga
     3001 ccacgccagc gccgcgaagc tcgaggtcac gcccgcgagc gcgatgagga tcgcggccgc
     3061 ctccagcgcg tacgtggcgg cgaaggtgcg gtcgaagatg cgcagcgccc tggcgtggat
     3121 gtacgacgca tcgtgcaact ccgcgcccgg cacctgtgcc acgactttcg caatgcgtgc
     3181 ctccaccgct cccgcgtcgg cgccggaggc aaggtggatg gcgatgtcgt tggcgaatgc
     3241 atcgttcgtc agcgcccggt agtcggcgag gtccacgagg agcgcgcccc aggtgcgcgc
     3301 gtagtcgcgg aagatcccgg ccacgcggaa ggaagcgctg cgccccgcga gaggtatcgt
     3361 cacgacttgc cccacgcgcc agccgaagag gtccaccgag gcttccgata tccacgccgg
     3421 gatggcgccg tcgcgcgcgg gcacggcggg cggatcgacc tggaagcccg cgagcagcgc
     3481 agcatccacg gggcgcgcca tcacggtgag aaggcccgtg cttccctcgg gcgcgaggcg
     3541 ctcgaagcgg atgggatcga tgcgggcaac ccccggcacg gcggcgatct gcgcctgcgc
     3601 ttccggcccg aacacgacgg catcgccctg cggcgcggcg cgcgcgaaga aatccgcctc
     3661 gagaatgccg gtgagccact ggtcgagcga gacgcggaag gaaaagacca tgatcgccat
     3721 ggcggccgtc agcgccacgc tcacgacgat gccggagacc atcgccgaga gatgcccggg
     3781 aaggctcttc acctgggcgc gcgcgagcgt tcgcagcggc gcgtcgctcg cgggaatgcg
     3841 atcgagcagc aggcgcgagg cggggctcac tgccatcacg gccgcggcga gcgccagccc
     3901 gatggagagg taaccgccga cgggaatctc gcccgccggc ggaagccacg cggcaaggat
     3961 cgcggccagc gcaagcgcga tgggccacgc ccagccgccg ggcggggaag gaagatcgag
     4021 atgccggtcc ttgagggcct gcgcgacgtc gaggcgggcc agcgatcgcg tgacggccaa
     4081 tgccgccgca gccgccgtga acactcccag gagcgcgatc accgcgagcg caactgcatc
     4141 gggcgcgaac gtgtcgcccg atcccgggaa gaaccccgcg ccgaggtcgt agccgaatcg
     4201 ctcgagcatg gcgcggctcg ccaccagccc cagcaccgtt cccaacgccg cgcccgccag
     4261 ccccaggagc ccgccctcga gcgcgaggaa ggcgcgcacg ccgttccccg tgaggccgag
     4321 cgcgcgcagg agcgcgaatt cctgccgcct tttcgccgcc tggagtgcaa gcgtggaaaa
     4381 gacgaggaat ccgccggtga aaagcgccat cagcgcaagc gccgtgaggt tcacgcggta
     4441 ggcgcgcgtg aggccggcgg tgcgcatggt gctctcgtcg gggcggacga tcgacacgcc
     4501 gggcggaagc ttcgcggcaa tctcgcgcag caccgtctcg cgcgcggcgc cgggggcgag
     4561 gcgcaggtcg atgcgatgga tcacgcccac gcgtccgaag gcgagttgcg cggcggcgat
     4621 gtccatcacg gcgatgggcc cgccgccttc cagcgcgggc agcgtcccgg cgatcttcaa
     4681 tcgaacctcg cccagcgggc cgcgcagcgc cacgtcgccg ccgaccgttg ccccggcctt
     4741 cgcgatggcc gcgggcgtga gccacgcggc gtgcgcatcg agaagcgtgg gcgtgccacc
     4801 ggcgcggctc gtggcgccct cggccaggaa ccccggctgc agccgggacg cgcggaacgg
     4861 atcgatgccc agcacgcgga tcgtcgtctc gcgtccggcc gccggcgctt cgagtccggc
     4921 cgcggttgcc tcgagctcga ccacgggaga ggcgacggcc accgccgggt gcagcgccac
     4981 ggcggcgtag gtggcatcgt cgaagccatt tcgcggcccg cgcacctgtt cgtccgccgt
     5041 gccggaaagc agtcgtgcgg cgcggtcgat ctcgtccagc gcagaggcat gcagcgtgtg
     5101 cacggcgccc gcaagcgcaa cgcccaaccc gatgcaggca atggccagca gcgagcgcca
     5161 gcggttgccg gagagcgatc cgcgaagaag cgttgcgacg agcgccgcgt tcatgccggg
     5221 ccgaggcggc gaccggccag gcgaaggacg cgatcggcgc aggcggccgc cacggccgag
     5281 tgcgtgacta gaaggccggc cgcgccctcg cgcttcaccg cgctgcgcaa cgcctgcagt
     5341 gcggacgcgg cgttgttctc gtcgaggttg ccggtgggct cgtcggccag caccagcgag
     5401 ggccggtgcg cgacggcgcg cgccacggcg acacgctgca tctcgccgcc ggaaagcgag
     5461 tacggccggc ggtcctcgaa gccggacatg ccgacggact cgagcgccgc gatggaccgg
     5521 ctgcggcgtt ccgcaccggg tacgccgttc agaagcagtg gaagccccac gttctcgccg
     5581 accgtgaggt aaggcagcag gtggaaggcc tggaagacga agcccacgtt ccggcggcgc
     5641 cacaacgcca gctcgtcgtc cgtcagcgcc gtaaccacgg tgccagactc cacccggagt
     5701 ctgacaccgg gcggagtctg actccgggtg gagtctgaca ccgtgacggt gccggcgtcg
     5761 gcccggtcga gaccggcgac gatgttgagc aacgtggatt tgcccgctcc ggattcgccg
     5821 aggatggcga ccagctcgcc gggcgcgacc gagaggtcca cggaatcgaa gaggggcgcg
     5881 ccacccggcc aggacttcgc gaggccgcgg acctcgagca tggccgggaa gccggctaga
     5941 accaggcgat gacgccgagg gccacgccgg ccagggcgct cccgccggcg ccccagagca
     6001 gccagtcctt cttcgtgagc acggtgatcg aggccagcgc gatggcgatc tgcagcaggg
     6061 tcatcgagat ggccagcatt tcgtgcgggt gcagcgcctt ctcgcttttc ctgttgctcg
     6121 ccttcgagtc gtcctcgagc ctgcgcgctt ccttctcgat gtccttcttt tccttcgcgt
     6181 agcgcgcggc ctcgacggtg tagaactcgg atttcttcgg gtccgcgctc atcgcggccg
     6241 cgaactccgc caggttctgc ttcatgctct tggcctggta gtagttccac tggtccgaag
     6301 cctcagcgcg cttcaggacc gcgtcgttct tgtagagcag cgcctcgttc tgcgtgtgcc
     6361 cgccgaggaa gctcacgacc gcgccgatgg tggcgaggac ggcggtgaag atcgcgatgc
     6421 gctggcccat gcccccgtgc tgcgcctggt gctcgacttc atggtcgtgg gggccgtgga
     6481 catggattcc gtgttccgac attcgccttg atttccttcc ggagtcgccg tggcggcgct
     6541 attgcccgct gatgtagctc tcgagctggg agatggtgaa gtcctggtcg gcgatgatgt
     6601 cggtcacgat gtcgcggatg gagaccatcc cgattacgcg gccctcgtcg accaccggca
     6661 ggtggcggat gttcctctcc gacatgagcc ccatgcagtc gcggctcttg gcgcgcgggc
     6721 tcacgcacac cacgttgggg gtcatgatct cctcgacgag cgtggtttcc gaggagcggc
     6781 ctctgaggat caccttgcgc gcgtaatcgc gctccgagag gatgccgcag agccggtcgc
     6841 cggacatcac gatggccgcg ccgatgttct cccgcgccat caccttgatc gcgtcgagga
     6901 cggtgtcctc gggcttcacc gacaccatcc gcgtaggctt gcctttcagc agttcagaga
     6961 cgttcttcat ggctttccct cctcgccagt gagactccgg gatcagctcg tgtacgccgc
     7021 gcctacatgt tcgggtagtt cggcccgccg ccgccttcgg gcaccaccca gtggatgttc
     7081 tgcgtcgggt ccttgatgtc gcaggtcttg cagtgcacgc agttctgcgc attgatctgc
     7141 aaccgcgaat tctgcccttc cgtgacgatc tcgtaaacgc ccgccgggca atagcgcgtc
     7201 tccggggcat cgtacagggc caggttgacg gctatgggga cggccgcgtc cttcagggtg
     7261 aggtgggccg gctggtcctc ctcgtgggcc acgttcgaga cgaacaccga ggagagacgg
     7321 tcgaacgtga gcacgccgtc gggctttgga tacacgggag gaacgcattc gcctttcggc
     7381 cgcagttgct cgtggtccgg gccggggtgg tgcagcgtcc acggcgcctt gccgccgaag
     7441 accacctggt cgatgccgac gagcagggac ccggcaacga gccccttggt catgaagggc
     7501 ttgaaattgc gcgcgcggta cagctcgtcg tgcagccagc tcttctcgaa cgcctcggga
     7561 aacgcggtga gctcgtcgcg cgaccggccc gcggccagcg catcgaacgc ggcatcggcg
     7621 gcgagcatgc ctgacttgat cgcggcatgg ctgcccttga ctttcgcggc gttgaggaag
     7681 cccgcgtcgt cgcccaccag cacgccgccc gggaagacca gcctgggcag cgcctgcagg
     7741 ccgccggcgt tgatcgcgcg cgcgccatag gccacgcggc ggccgccctc gaggaaggcg
     7801 cgcatggccg ggtgcgtctt gaagcgctgg aattcctcga agggggagag gtaggggttg
     7861 gtgtagccca gcccgacgac cagtcccacg gacacctggt tgtcctccag gtggtacatc
     7921 cacgagcccc cgtaggtcga attgtccagc ggccatccgg caccgtggat cacgaggccg
     7981 ggccggtgct tcgcgggatc gatctcccag agctccttca ggccgaggcc gtaaacggcc
     8041 gggtcgcgtc cctcgcgcag ccggaagcgc tcctgcagcc ccttgccgag attgccccgg
     8101 cacccttccg cgaacacggt gtacttcgcg tgcaattcca tgccgggctg gtaacgggtg
     8161 gttctctcgc cgttcttccc gatcccgacg tcgcccgtgg ctacgcccat gacgcgtcct
     8221 tcctcgtcga agagcacctc cgccgctgcg aagccgggat agatctcgac gcccagtgct
     8281 tcggcctgcg cgccgagcca gcggcagagg ttgccgaggc ttgcggcgta atagccgaag
     8341 ttgccgaaac aggcgggcag gagccagttg gggacctgcg tggtgcccgt ctcggagaag
     8401 aacaggaagc ggtcctcggt caccggttgc ttgagcggcg cgcctttttc cttccagtcc
     8461 ggaaagagtt ccgcgagcgc cttcggatcg atgaccgcgc ccgagaggat gtgggcgccg
     8521 atctccgaac ccttctcgat gaggcacacc gagacctctg cgtccctggc ggcggcgagt
     8581 tgcctcaggt ggatggccgt cgcgaggccg gcggggccgc cacccacgac aaccacatcg
     8641 aattccatcg attcgcgggt cattccggcc tccagcggcc ttggaaacgt ttgatttggc
     8701 tcatgtcgcg agttcgtgat gcctttctaa tggcgaatgc ggtgccagac aagacacgga
     8761 gtctgacacc atggagatcg catgggaccc tgggcaattt cgctgctcat gataacgggc
     8821 ttcgccggat tcgctgtgct ggcgtggcgc aagcttgcca tcgtggtcgc gctccagccc
     8881 gaggtgcgct gggaccggcc aggggagcgg attgcgcggc tcctgacgat gggcttcggg
     8941 caatcgcgca tggtcgccgg cgaatggaag ccgggaatca tgcacgcggc gatcttcctc
     9001 ggcttcatgg cgctgctggt gcgcaagctc aacctcatcg ccatcggcta cgaccctctc
     9061 gcgctcatcc ccggctggcc gggggcggtc tacgcggcga tcaaggaact ggtcgaggtg
     9121 ggcgtgctcg cggccctcgt ctatgcgttc tggcggcgct tcgtgctgaa gccgaagcga
     9181 ctggagccca accgcgaggc gatcctcatc ctgtcgctca tcgccgtgat cgtcgtcacc
     9241 gatttcctct tcgacggttt ccgcttcgtg aaatacgcgg ccgacgccgg catcacccac
     9301 gagcgcggct tcgccccggt gggcgcggcg ctggccgtcg ccttcgcggg cctctccccg
     9361 ggcgcgatcg acctcgggct gcacgcctcc tactgggtac agatggtcac ggtgttcacg
     9421 ttcctcgtgc tgctcccggc cggggagcat ttccatatcg tgacggcact tcccgccgtg
     9481 ttcctcgcac gcgcgacgcc gctcaaccgc gtgccctccg tcgacctcga gagaatcatg
     9541 gcggacgacg ccggcgacga catgaaggtg ggcgcgaaga cggcggccga cctcctgtgg
     9601 aaggaagcct tcgacccctt tacctgcacc gagtgcgggc ggtgcaagga ttcctgcccc
     9661 accttcctca ccggcaagcc gctggctatg aagtgggtga acgactcgct caagcaccac
     9721 ctgatggaaa agcgcgagcc gatcgtggcc ggaaaagagg cggatcttcc gccgctcgtg
     9781 ggcggcgtga tttcggaaga gaccctctgg gcgtgcacca cctgcggcta ctgcgagcgc
     9841 tcgtgcccca tcggcctcga gcacctcggg cgcttctacc gcctgcgcca gcaccaggtg
     9901 atgatggaag gcgccttccc gcaggaactg aaggccgtct tcgacgccta cgagagccag
     9961 tccaatccgt gggggctgcc ggccgatacg cgcggcgact gggcgaagga tctcggcgtg
    10021 ccgctcctcg agaacgcgca acaggcaagg gagatcgact ggctcttcta cgtgggctcg
    10081 gcgcagtcct tcgacttgcg tgcgcagaag acggcgaagg ctttcgtgaa gatcctcaag
    10141 gccgcgggat tgcgcttcgc gatcctgggg ccgcgcgagg gctcgaccgg cgagtgcgtg
    10201 cgccgcgccg gcaacgagat gctcttccag tcgcttgcgc aggccctggt ggagacgctg
    10261 aacggcctgg gtgtcgcgcg catcgtcacc tgcgatccgc acgccttcaa ctcgctcgcc
    10321 catgaatacc cggagttcgg cgggaagtac gaggtgatcc accacacgca gctcatcgac
    10381 cggctcatcc gcgacggaaa gatcgcgccg cgccgcgtct tcgagggcgt catctaccac
    10441 gagccctgct atctcgcgcg ccacaacggc gagtacgagg ccccgcgccg cgtgctggcc
    10501 gccgtctcgc gcgacacgcc gccggagttc gtcatggccc gcgacaaggc gatgtgctgc
    10561 ggcgccgggg gagggcgcat gtggatggag gagcacaccg gcaagcgcat caacgtgctg
    10621 cgcgccgagc aggcgctgga gcgtgccccg aaggccatcg tcacggcgtg tccctactgc
    10681 gcgatcatgc tgagggatgg cgtggcgcag cttggccacg cggacgacct gccgacgcga
    10741 gacatcgcgg agctggtggc cgaggcgatc gaaccggcaa cctgagcgca gtcgaggtgg
    10801 ccggggagtc gcgatcggaa tggaagggag gcgtcgggga aagtcgatcg caatcctggg
    10861 cgattgctag gtaggcgtcg ggaaaagtcg atcgcaatcc tgggcacgca agcgagaccg
    10921 tcggatgggt gcccggaata gtgtcaccca tgctcccgga taggaccgaa caagccagac
    10981 ctcgaatttc cacccatcta cagacgaggc cgctgcgctg cctc
//
LOCUS       DASUEV010000115         4427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1064312, whole genome shotgun sequence.
ACCESSION   DASUEV010000115 DASUEV010000000
VERSION     DASUEV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064312"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFV55_03825"
     CDS             <1..874
                     /locus_tag="VFV55_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX4943456.1"
                     /translation="FGAKKDEIVGPVLSDFGYHIIRVTDVKPEKGKSLAEATPEIEAE
                     LKKSVAARRFAEVAEGLTNMVYEQSTSLKPAADQYKLTVQQTNWFAKAGGAPPLLANP
                     KLLAEIFSDNAIKAKRNTAAIEVAPSVLVAARVLEFKPAEQRPYDAVRALIEARTKRE
                     EALKLAVADGEAKLKAAAEGKDAGLKWPAPLAVSRQKPGGLSPAVLDKVFRADAKKLP
                     AVVGLATPAGYSLVRITKVMEVDAIDDAKRKALAERLKQTLAMSELESTLKSLRGTIG
                     VEIRKDALDKKASP"
     gene            complement(1025..1813)
                     /locus_tag="VFV55_03830"
     CDS             complement(1025..1813)
                     /locus_tag="VFV55_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4943457.1"
                     /translation="MGFMQGKRILVTGMLSNRSIAYGVAKALVREGAEVAFTYQGEGI
                     RERVAELAREFGSAPLYPCDVSDDAQIDALFVALARHWDGFDGLLHAIAFSPREALKG
                     EFHESVTREAFRIAHDVSSYSLAALARGAAPYMKGRGGSIVTLTYLGSSRSIPRYNVM
                     GLAKASLEACVRYLAAGLGPQGIRVNAISAGPIKTLAAAGIGDFGKIFRFVEKYAPLR
                     RNVTADEVGNVAAFLLSDLASGITGEVTYVDAGFNTTTAGLADD"
     gene            1965..3743
                     /locus_tag="VFV55_03835"
     CDS             1965..3743
                     /locus_tag="VFV55_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943458.1"
                     /translation="MLAAVPAAAADPAKVFRYAFPIAETGFDPAELSDLYSGNLIANI
                     FDTPLEYDYLARPITLVPNTLAEMPVITEDGTLYTMRVKPGIHFADDPAFKGQSRELT
                     AQDYVYSIKRLFDPKRKSPNLYLLEGLVAGMDEILAAARKANRMDYDTPVEGLRALDR
                     YTFQIRLKQPNYNFLYYLAYCNLTCAVAREADEFYGDRIGENPVGTGPYRLTFWKRSS
                     KMVFEANPGFREQHFAGEPAPGDARAEAALAANKGKRLPMVGRIEVYIVEESQPRWLA
                     FLNAEHDYIERVPDEFSNVVVPNNRLAANLARRGIQLDRQPGMEITYSYFAMKDPVVG
                     GYTPEKIALRRAIVLGQDVGAEIRIARKNQAIPAYSPIGPGAMGYDPDFRSTATEYNP
                     AKAKALLDMYGYADCDGDGWRDLPRKSADEPCKPFSVEYASRPDGPQKPLDENWKKNM
                     DDIGIRMTFRKAKWPDLLKESKAGRLQMWGLGWSAAIPDADMFFVMLYGPNEGQANHS
                     RFSLPEFDRLYEQAKRLPNGPERNAIYREMNRLFLVYAPWRLGVHRYYNDLVHPWVLG
                     YYRHPVMRGTWKFLDIDLARLAAAGR"
     gene            3748..>4427
                     /locus_tag="VFV55_03840"
     CDS             3748..>4427
                     /locus_tag="VFV55_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943459.1"
                     /translation="MRPLTCVVRALAATVLVAAFAAGAAPLKVLRVPFNIAETNFDPA
                     FASDTYSNTVIDEIFEPPLTYDMLARPYKLKPQTAEAMPEIAEGGRIYTIRLRKGIYF
                     ADDPAFGGKRRELEAKDYEYAIKRLIDPKIASPNAWLVEGRIDGIDDAAAAAKKAGHL
                     DYDAPIRGIEMPDRHTLRIRLAKPDYNFLYILAMGSLGAQAREVVERYGTDIGAHPVG
                     TGAFVLAE"
BASE COUNT          804 a         1521 c         1417 g          685 t
ORIGIN      
        1 cttcggcgcg aagaaggacg agatcgtggg cccggtgctg tcggacttcg gctatcacat
       61 catccgcgtg accgacgtga agcccgagaa gggcaagagc cttgccgagg ccacgccgga
      121 gatcgaggcg gagctgaaga agagcgtcgc cgcgcgccgc tttgcggaag tggccgaggg
      181 cctcacgaac atggtctacg agcagtcgac gagcctcaag cccgccgcgg accagtacaa
      241 gctcaccgtc cagcagacca actggttcgc gaaggccggg ggcgccccgc cgctgctggc
      301 caacccgaag ctcctcgcgg agatcttctc ggacaacgcc atcaaggcca agcgcaacac
      361 cgcggccatc gaggtggcgc ccagcgtgct ggtggccgcg cgcgtgctcg agttcaagcc
      421 ggccgagcag cgcccgtacg atgcggtgcg cgcgttgatc gaggcgcgta ccaagcgcga
      481 ggaagcgctc aagctcgccg tcgccgacgg tgaagcgaag ctcaaggccg cggccgaagg
      541 caaggatgcc gggctcaaat ggcccgcgcc gctcgcggtc agccgccaga agcccggcgg
      601 gctctccccg gcagtgctgg acaaggtctt ccgggcggat gcgaagaagc tgcccgccgt
      661 cgtcggcctg gcgacgcccg ccggctactc cctggtgagg atcaccaagg tgatggaagt
      721 cgatgccatc gacgacgcca agcgcaaggc cctggccgag cggcttaagc agacgctcgc
      781 gatgagcgag ctggagtcca cgctcaagag cctgcgcggc accatcggcg tcgaaatccg
      841 caaggacgcg ctcgacaaga aggcctcgcc ctgatcagga aaaggggtca ggttactttt
      901 cagcccgctg tcttgcgacg gcaatacgac agaaaagtaa cctgacccct tttccgacct
      961 cttttccggt ttgcctattt tttgtgcaaa gtaacctgac cccttttcga ccccttttcc
     1021 tttcctagtc gtcggccaga cccgctgtcg ttgtgttgaa gccggcgtcc acgtaggtca
     1081 cctcgccggt gatgccgctc gccaggtcgg agagcaggaa ggcggcgacg ttgcccacct
     1141 cgtcggccgt cacgttgcgg cgcaacggcg cgtacttttc cacgaagcgg aagatcttgc
     1201 cgaaatcgcc gatgccggcg gcagccagcg tcttgatcgg gccggccgag atcgcattga
     1261 cgcggatgcc ctgcgggccg agccccgccg ccaggtagcg cacgcacgcc tccaggctcg
     1321 ccttggcgag acccatgacg ttgtagcggg gaatggagcg cgacgacccg aggtaggtga
     1381 gggtgacgat cgacccgccg cggcccttca tgtacggcgc cgcgcccctg gcgagcgcgg
     1441 ccaggctgta gctggacacg tcgtgggcga tacggaacgc ttcccgcgtg acgctctcgt
     1501 ggaactcgcc cttcagcgct tcccgcgggg aaaaggcgat cgcgtgcaac aggccgtcga
     1561 aaccgtccca gtgcctggcg agggcaacga acaacgcgtc gatctgggcg tcgtcgctga
     1621 cgtcgcaggg gtagaggggg gcgctgccga attcgcgggc gagctccgcc acgcgttcgc
     1681 ggatgccttc accctggtag gtgaacgcga cctcggcgcc ttcgcgcacc aaggccttgg
     1741 ccacgccgta ggcgatggac cggttgctga gcattcccgt gacgaggatg cgcttaccct
     1801 gcatgaaccc catttcgggc gtcctttccc agtagaattg cgacaaattc gggcgtggag
     1861 agcggggtcg aattctatcg cattccgtcc ccttccccgc cgtgccgccc gtgaggtccg
     1921 tatccatgct gttcgcgcgc cttcttgcac cactcgcatg cgcaatgctt gccgccgtgc
     1981 ccgccgcagc ggccgacccg gccaaggtct tccgctacgc gtttcccatc gcggagacgg
     2041 gcttcgatcc ggccgaactc tcggatctct attcgggcaa cctcatcgcc aacatcttcg
     2101 acaccccgct cgagtacgac tacctggcgc ggccgatcac gctcgtgccc aacacgctcg
     2161 cggagatgcc ggtgattacc gaggacggca cgctgtacac gatgcgcgtg aagccgggga
     2221 tccacttcgc cgacgacccg gcgttcaagg gccagtcacg cgagctcacc gcccaggact
     2281 acgtctactc catcaagcgc ctgttcgacc cgaagaggaa gtcgcccaac ctctacctgc
     2341 tcgagggcct ggtcgccggc atggacgaga tcctggcggc cgcgcgcaag gcgaaccgca
     2401 tggattacga cacgccggtc gagggcctgc gcgcgctcga ccgatacacg ttccagatca
     2461 ggctcaagca gcccaactac aacttcctct actacctcgc ctactgcaac ctgacctgcg
     2521 cggtcgcacg cgaggccgac gaattctacg gcgacaggat cggcgagaat cccgtcggaa
     2581 ccggcccgta ccggctgacc ttctggaaac gctcctcgaa aatggtgttc gaggccaacc
     2641 cggggtttcg cgagcagcat tttgccgggg agcccgcgcc gggcgatgcg cgggccgagg
     2701 ccgcgctggc tgccaacaag ggcaagcgac tgccgatggt gggcaggatc gaggtctaca
     2761 tcgtggagga atcgcagccg cgctggctcg ccttcctcaa cgccgagcac gactacatcg
     2821 agcgcgtgcc cgacgaattc tcgaacgtgg tggtgcccaa caatcgcctc gcggccaacc
     2881 tcgccaggcg cggcatccag ctcgaccggc agccgggcat ggagatcacc tactcgtatt
     2941 tcgcgatgaa ggacccggtc gtcggcggct acactccgga gaagatcgcg ctgcgccgcg
     3001 ccatcgtgct gggccaggac gtcggcgccg agatccgcat cgcgcggaag aaccaggcga
     3061 tccccgccta cagccccatc ggccccggcg ccatgggcta cgacccggat ttccgttcca
     3121 cggccaccga gtacaacccg gccaaggcca aggcgctcct cgacatgtac ggctacgcgg
     3181 actgcgacgg cgacggctgg cgcgacctgc cacgcaagag cgcggacgag ccgtgcaagc
     3241 cgttctcggt cgaatacgcc tcccgtcccg acggcccgca gaagccgctc gacgagaact
     3301 ggaagaagaa catggacgac atcggcatcc ggatgacgtt caggaaggcc aagtggccgg
     3361 acctgctcaa ggaatcgaag gccggccggc tgcagatgtg gggcctgggc tggagcgccg
     3421 ccattcccga tgccgacatg ttcttcgtga tgctctacgg tcccaacgag ggccaggcca
     3481 accactcgcg cttcagcctg ccggaattcg atcgcctcta cgagcaggcc aagcgcctgc
     3541 ccaacgggcc ggagcgaaac gcgatctacc gcgagatgaa ccggctcttc ctcgtctacg
     3601 cgccgtggcg cctgggcgtg caccgctact acaacgatct cgtgcacccg tgggtgctgg
     3661 gctactaccg gcacccggtc atgcgcggca cctggaagtt tctcgacatc gaccttgccc
     3721 ggctcgccgc cgcgggcagg tagcgccatg cgcccgctca cctgcgtcgt tcgcgccctc
     3781 gctgccacgg tgctcgtcgc ggcgtttgcc gcgggagcgg cgcccctgaa ggtactgcgc
     3841 gtcccgttca acatcgcgga gacgaacttc gacccggcgt tcgcgtcgga tacgtactcc
     3901 aacacggtga tcgacgagat cttcgagccg cccctcacct acgacatgct cgcgcggccg
     3961 tacaagctga agccgcagac cgccgaggcg atgccggaaa tcgccgaggg cggaaggatc
     4021 tacaccatcc ggctgaggaa gggcatctac ttcgcggacg atccggcctt cggcggcaag
     4081 cggcgcgagc tggaggccaa ggactacgaa tacgcgatca agcgcctcat cgacccgaag
     4141 atcgcttcgc ccaacgcctg gctggtcgag ggcaggatcg acgggatcga cgacgccgcg
     4201 gctgccgcca agaaggcggg gcatctcgac tacgatgcgc cgatccgcgg aatcgagatg
     4261 ccggaccgcc acacgctgcg catccgcctc gccaagcccg actacaactt cctctacatc
     4321 ctcgccatgg gctcgctggg tgcgcaggcg agagaggtgg tggagcgcta cggcacggac
     4381 atcggcgcgc acccggtggg cacgggagcc ttcgtcctcg cggaatg
//
LOCUS       DASUEV010000116         9305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1162500, whole genome shotgun sequence.
ACCESSION   DASUEV010000116 DASUEV010000000
VERSION     DASUEV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162500"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VFV55_03845"
     CDS             <1..1491
                     /locus_tag="VFV55_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4943460.1"
                     /translation="LAQQLQAADSPGGVLFETFHRRKDGSTFPVEVSARSLEIGGKLY
                     RQSFVRDITTRRQAEDAIRESEVRFRTVVESAPDGIFIQTGGRFAWLNREAMRIFGIA
                     GMEAARGQPVAERFHPDSRELARARIRLVNEERRSVGAGELAVLQPDGSRVDVETSAV
                     PFTYEGEDGALVYVRDITDRKHALEELRRSEHRFRTLVEGVPNIAVQGYDRERRVVFW
                     NAASEALYGFTPEEAAGRKLEDLIIPPEMREAVVAATTRWVTDGVPIPASELVLRRKD
                     GSPVTVFSSHAMSVNQRGDPEMYCIDVDLTALKRAEAESVEWKHRYEAAILASGQLLH
                     DWNPKTGEMSILGDTERMLGYRTDEMAGGLPRWREQIHPDDREAFDRGIAHAIENRAP
                     FHQRYRVARKDGIWLTMQDDGYFLLDASGAISRMVCFVVDVTERSRVQSRIEEQLDEL
                     RRWHAAMLGRETRILELKREVNGLLAAAGQSPRYGVTGRDGAKATA"
     gene            1488..3128
                     /locus_tag="VFV55_03850"
     CDS             1488..3128
                     /locus_tag="VFV55_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HEX4943461.1"
                     /translation="MIESPALSLVHNASLLLAMALVYDVLAGKSAPLSHQARRQVLLG
                     LLVGAIGMAVMLTPWQYVPGVAFDTRSVLLGVTGLFFGWIPTVIAMAMTAALRLYQGG
                     LATGAGVAVIVASGCLGLAWRRWHRGALEDISFPSLYVLGITIHVTMLALMFLLPREM
                     ALRVLAAITLPVLVIYPFATALLGALLSARLRRHRLGGRLVESEERLRLALAAANQGL
                     WDLDIRTGEVIVSTEYATMLGYDPAGFRETSASWNQRVHPEDHERVAGIYRDYVAGAI
                     PEYRVEFRLRMQDGGWLWVLSQGSIVARDAAGAPLRMLGTNTDITGRRKSEQHLREAQ
                     AEAQRLLAESDQSRLALLSVVEDLKAAEEKLGRSEAFYRSLFENMHEGFAYCRMVYED
                     GEPRDFVYLRVNESFLRMRGLSRVEGRAASEVIPGIRDSNPELLENYGRVAASGNPEI
                     FEVNIARLERWYLVSVFSPEKDHFVAAFTDITQRKEAELENERRLAELKRWHEVTLDR
                     EERVMQLKAEVNELRRRLGEAPRFGLPGPAPQPPGGGP"
     gene            3125..4144
                     /locus_tag="VFV55_03855"
     CDS             3125..4144
                     /locus_tag="VFV55_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4943462.1"
                     /translation="MKPGRGVAIAVVLAVAVVVAGALWWAAPFRQAAPPVAPEKLAIA
                     VSGTYIGSGLVLVAAARGYFAQEGLEVTLQQHTSGRDALDAVLGNHADIATAGDTPLM
                     FAVMKGAPVSVVATIFTAGRAHGIVARRDRGIATPADLAGKRLGATFGTDAHFVADVM
                     LAAHGKSLSSVQLENIKPEDAPAALSSGRVDAVATWEPWIGMARKAAGDNAIAFLTDP
                     GFLIGFNLAGRKDWVGANQEKLRRLLRALVRAEQLVAEKPQEARTLILEATGTDRSIF
                     DAGGPSYRFQVRLDQSLLIMLEDQGRWAINEGLTERRQVPNLLDALDLRPLEAVRPAA
                     ISIVR"
     gene            4150..5607
                     /locus_tag="VFV55_03860"
     CDS             4150..5607
                     /locus_tag="VFV55_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4943463.1"
                     /translation="MRIRTQAYLSALISVAIVGVIVATLLAFETQTQLAIARLEFANR
                     IYSEGVSGLRLATTEYFLYRQPRARQQWLQRHASLTRLLASDILEGSPEQASLEALQA
                     RNRYVGEVFAHLAALYERPATGATTQEYERRLVTQIMLATQDMVVDAALLATRNHERL
                     VAARRAASSLILALVLLAGVIVLVNSAMTLSQLVRPILLLKQGAEVLAGGGLAFRTNI
                     AVDNEIGELARSFDRMAAQLAESHDALERKGAQLEEANRELEAFSYSVSHDLRAPLRG
                     IDGWSLALLEDFGDGLHEKARGYLGIVRSETQRMGQLIDDLLMLSRVGRAGIRHEPLD
                     LSAIATSVAARLEQAEPARRFEFAIQQGMRANGDEQLLRIALANLFDNACKFTRPRAV
                     ARIEFGAEEEQDPATGARRAVYFVRDNGVGFDMARAKNLFGAFQRLHTTSEFPGTGIG
                     LATVKRIIHRHGGTISAEAQVGRGATFRFTLEEAA"
     gene            5604..6044
                     /locus_tag="VFV55_03865"
     CDS             5604..6044
                     /locus_tag="VFV55_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4943464.1"
                     /translation="MNMKPILLVEDNPSDVGLTRRAFEKSAIRNTLVVAEDGQEALDY
                     LFAEGRHAGRAPGEAPMLVLLDLKLPRVDGLEVLRRMRADPRTRRIPVVILTSSSEEQ
                     DIALGYDLGTNSYIRKPVDFHNFADVIRQLGMYWLVMNEPPPDA"
     gene            6037..7881
                     /locus_tag="VFV55_03870"
     CDS             6037..7881
                     /locus_tag="VFV55_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4943465.1"
                     /translation="MPEAARGEPLSLLVVEDSASDAGLMVRHLERNGFDVADRRVETE
                     DELRAALREGPWDVVLCDFNVPGFDAGAALAALHETGLDIPFIVVSGVIVEGAAIELM
                     RAGASDYVMKENLARLAPVVRREVEEARGRAASRRAQAALAATLQRHAAAIDSALDGY
                     AALDGGHRILEVNDALSAMTGRSREELLQLRVDELFAAGAIAASQAFPSREGGGHARF
                     DSQWRRKDGSTLDVELSVTCHGGEDIEIFLFAHDITMRKQREAELGGLTRESERARLA
                     LLGVLEDQREIAEELRESEEQFRGMLEHNVSAMFLIEDGNLTYANPRTGEILGYTSDE
                     MTGKPFISFVVDEDRQMIARVVEQLISGKLGKAERNFKAIRKDGSHVDIGASATRAML
                     RGKVMILGAAQDIGERKKAREEIDRYIVRLESSMQGTLEAVSHMVELRDPYTSGHERR
                     VGDLAAAIAAEMGLPDAVIKGLRLTGYVHDIGKISIPAEILSKPGRLTPLEYELIKSH
                     SQAGFEVLKGVDFPWPVAEAILQHHERADGSGYPRGLKGEEIILEARVIAVADVLEAM
                     GSHRPYRPSLGLEAALAEIEKNAGRLYDATAAAACLRLFREKGYSLGA"
     gene            complement(7916..9223)
                     /locus_tag="VFV55_03875"
     CDS             complement(7916..9223)
                     /locus_tag="VFV55_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX4943466.1"
                     /translation="MSQQEHRTAVPSGVADILQAFVQFPAPMALVNLEGTAVLVNAAF
                     SSRFGEARVVPESLRELLRGEGGERRVALRSPGLEQAVEVRARAIPMPDRILLILDES
                     GGPRRHDEMEGLRERVSQLERLAATDHLTGAWNRSHFDRLIDAEIARSTGSRQPVSLV
                     LLDIDHFKRINDTFGHGMGDSVLRELVRLVQSRIRASDVLFRWGGEEFTVLVASAGYR
                     GAERVAENLRAAVAGHEFRGVGTVTISLGVAEHAGGEDPQSWFRRLDETLYAAKNGGR
                     NRVVVDRRGSSDAWAAEVGVSALHLEWQEAYECGDATIDGEHEQLFVLANRLIDAVAR
                     GEEEPATFREALDVLLAHVQRHFADEEAILARCHYADLEQHKAAHKGLLRRAGYLKER
                     AEAGEASLGSVVEFLAQDVVARHLMTVDRAFFPLFDKRPPVPA"
BASE COUNT         1497 a         3206 c         3229 g         1373 t
ORIGIN      
        1 ctggcgcagc agctgcaggc cgccgacagt cccggcggcg tgcttttcga gacgttccac
       61 cggcgcaagg acggcagcac cttccccgtg gaggtcagcg cgcggtccct cgagatcggc
      121 ggcaagctgt accggcagag cttcgtgcgc gacatcacca cccgccggca ggccgaggat
      181 gccatccgcg agagcgaggt gcgattccgc accgtcgtcg agtcggcccc cgacggcatc
      241 ttcatccaga ccggcgggcg cttcgcgtgg ttgaaccggg aggccatgcg catcttcggc
      301 atcgccggca tggaggccgc gcgcgggcaa ccggttgccg agcgtttcca tcccgattcc
      361 cgggagctgg cgcgtgcgcg catccggctg gtgaacgagg agcgccgttc cgtgggcgcc
      421 ggcgagctgg ccgtgctgca accggacggc tcgcgggtcg acgtcgaaac gagcgccgtt
      481 ccgttcacct acgagggcga ggacggagcg ctcgtgtacg tgcgcgacat caccgaccgc
      541 aagcacgccc tggaggaact gcgcaggagc gagcatcgct tccgcacgct ggtggagggc
      601 gtgccgaaca tcgccgtcca gggctacgac cgcgagcgcc gggtggtctt ctggaacgcg
      661 gccagcgaag cgctctacgg cttcacgccg gaggaggcgg ccggccgcaa actcgaggac
      721 ctgatcattc ccccggagat gcgcgaggcc gtggtcgccg caacgacgcg atgggtaacg
      781 gatggcgtgc cgatacccgc gagcgagctg gtcctgcgcc gcaaggacgg ctcgcccgtc
      841 accgtgttct cgagccacgc catgtcggtc aaccagcgcg gcgacccgga gatgtactgc
      901 attgacgtgg acctgacggc gctcaagcgg gccgaggcgg aatcggtcga atggaagcac
      961 cgctacgagg cggccatcct ggcgagcggg cagttgctgc acgactggaa tccgaagacg
     1021 ggcgagatgt cgatcctggg cgacacggag cgcatgctcg gctaccgcac cgatgagatg
     1081 gccggcggcc tcccgcggtg gagggaacag atccaccccg acgaccgcga agcgttcgac
     1141 cgagggatcg cccatgcgat cgagaatcgc gccccgttcc accagcgcta tcgcgtcgcg
     1201 cggaaggatg gcatctggct caccatgcag gacgacggct acttcctgct cgacgcatcc
     1261 ggcgcgatct cgcgcatggt gtgcttcgtc gtggacgtca ccgagcgcag ccgggtgcaa
     1321 agccgcatcg aggaacaact ggacgagttg cgccgctggc acgcggccat gctcggccgc
     1381 gaaacgcgca tcctcgagct caagcgcgag gtgaacggcc tgcttgcggc cgccggccag
     1441 tcgccccgct atggcgtcac cgggcgcgac ggcgcgaagg cgacggcgtg atcgagtcgc
     1501 ccgcgctctc gctggtccac aacgcctcgc tgctgctggc catggcgctg gtgtacgacg
     1561 ttcttgccgg aaagtccgcg ccgctctccc accaggcccg gcgccaggtc ctgctcggcc
     1621 tgctcgtcgg cgccatcggc atggccgtga tgctgacccc gtggcagtac gtcccgggcg
     1681 tggccttcga cacgcgctcc gtgctgctgg gcgtcaccgg cctgttcttc gggtggatcc
     1741 cgacggtcat cgcgatggcc atgaccgccg cgttgcgact gtaccagggc ggcctggcca
     1801 cgggggccgg cgtggcggtc atcgtcgcct ccggatgcct gggcctcgcg tggcgccgct
     1861 ggcaccgcgg agcgctcgag gacatttcct tcccctcgct gtacgtgctc ggcatcacca
     1921 tccacgtgac catgctcgcg ctcatgttcc tgcttccgcg ggagatggcc ctgcgcgtgc
     1981 ttgcggcgat caccctgccg gtcctcgtga tctatccgtt tgccaccgcc ctcctcggcg
     2041 ccctgctttc cgcccggctt cggcgccacc gcctgggggg ccggctcgtg gaaagcgagg
     2101 aacgactccg gctggcgctt gccgccgcca accaggggct ctgggatctc gacatccgca
     2161 ccggcgaggt catcgtgagc accgagtacg ccaccatgct cggctacgac ccggccggct
     2221 ttcgcgaaac ttccgcgtcg tggaaccagc gcgtgcatcc cgaggaccac gagcgcgtgg
     2281 ccgggatcta ccgcgactac gtggcgggag cgatcccgga ataccgcgtc gagtttcgcc
     2341 tgcgcatgca ggacggcggc tggctctggg tcctctccca gggcagcatc gtcgcccgcg
     2401 acgcggcggg cgcgcccctg cgcatgctcg ggaccaacac cgacatcacc gggcgcagga
     2461 agtccgagca gcacctgcgc gaggcgcagg ccgaggcgca gcgcctgctg gccgaatccg
     2521 accagtcgcg gctcgccctc ctgagcgtcg tggaggacct caaggcggcc gaggagaagc
     2581 tgggccgcag cgaggcgttc taccggagcc tgttcgagaa catgcacgag ggcttcgcct
     2641 actgccggat ggtctacgaa gacggcgagc cgcgcgactt cgtctatctg cgcgtcaacg
     2701 aatcgttcct gcgcatgcgc gggttgtcgc gagtggaggg tcgcgcggcg agcgaggtca
     2761 tcccgggcat ccgcgattcc aacccggagc ttctcgagaa ctacggccgc gtcgcggcgt
     2821 ccggcaatcc cgagatcttc gaggtgaaca tcgcgcgcct cgaaaggtgg tacctggtat
     2881 ccgtcttcag tcccgaaaag gatcacttcg tggcggcgtt caccgacatt acccagcgca
     2941 aggaggcgga actggagaac gagcgccgcc ttgccgaact gaagcgatgg cacgaagtca
     3001 cgctcgatcg cgaggagcgc gtcatgcagc tcaaggccga ggtaaacgag ctgcggcgca
     3061 ggctgggtga agcgccacga ttcggcttgc cgggtccggc accgcagccg ccagggggag
     3121 gcccgtgaaa cccggcagag gcgtcgccat cgcggtcgtg cttgcggtcg cagttgtcgt
     3181 ggccggagcg ctatggtggg cggcgccatt ccgccaggcc gcccctcccg tcgcgccgga
     3241 gaagctcgcc atcgcggtga gcggcaccta catcggctcc ggtctcgtgc tggtcgcggc
     3301 ggcccggggc tacttcgcac aggagggact ggaggtcacg ctccagcagc acaccagcgg
     3361 ccgcgatgcc ctcgatgcgg tgctcggcaa tcacgccgac atcgcaaccg ccggcgacac
     3421 gcccctgatg ttcgcggtga tgaagggagc gccggtttcg gtggtggcga cgatcttcac
     3481 cgccggccgg gcccacggaa tcgtcgcgcg ccgcgaccgg ggcatcgcca cgcccgcgga
     3541 tctcgcgggc aagcggctcg gcgcgacgtt cggcaccgac gcgcacttcg tggccgatgt
     3601 catgctggcg gcgcacggga aaagcctgtc gtcggtccaa ctcgaaaaca tcaagcccga
     3661 ggatgcacct gcagccctgt cttcgggtcg ggtggacgcc gtggcgacct gggagccgtg
     3721 gatcggcatg gcgcgcaagg cggccggaga caacgcaatc gccttcctca ccgaccccgg
     3781 gttcctgatc ggcttcaacc tggccggacg caaggattgg gtgggcgcga accaggagaa
     3841 gctgcgccgc ctcctgaggg cgctcgtccg cgccgagcaa ctggtcgccg agaagccgca
     3901 ggaggcccgc acgctgatcc tcgaggcaac cgggaccgac cgttccatct tcgacgccgg
     3961 cggccccagc tatcgcttcc aggtgcggct cgaccagagc ctgctgatca tgctcgagga
     4021 ccaggggcgc tgggcgatca acgagggcct cacggagcgc aggcaggtgc cgaacctgct
     4081 cgatgcgctc gacctccgcc cgctggaagc ggtcaggccc gcggcgatct cgatcgtgcg
     4141 ctgaccccga tgcgcatccg tacgcaggcg tacctgagcg ccctcatttc cgtggccatc
     4201 gtcggcgtga tcgtcgcgac gctgctggca ttcgagacgc agacgcagct tgcgatcgcc
     4261 cgcctcgagt tcgccaatcg catctattcg gagggcgtga gcggcctgcg gctggccacc
     4321 acggagtact tcctgtaccg gcagccccgg gcccggcagc aatggttgca gcgccatgca
     4381 tcgctgaccc ggctgcttgc cagcgacatc ctggagggat cgcccgagca ggcgagcctg
     4441 gaggcgctgc aggcgcgcaa ccgctacgtg ggcgaggtat ttgcccacct cgcagcgctc
     4501 tacgagcgcc ccgcgacggg cgcgacgacg caggaatacg agcgccggct ggtgacccag
     4561 atcatgctcg ccacgcagga catggtcgtc gacgcggcgc tcctcgccac ccggaaccac
     4621 gaacggctcg tcgcggcccg gcgcgcggcg agcagcctga tcctcgcgct ggtcctcctg
     4681 gcgggcgtca tcgtcctcgt gaactccgcg atgaccctga gccagctcgt caggccgatc
     4741 ctgctcctga agcagggcgc ggaagtcctc gcggggggag gcctcgcgtt ccggaccaac
     4801 atcgccgtgg acaacgagat cggcgagctc gcgcgatcgt tcgaccggat ggcggcgcag
     4861 ctggccgagt cgcacgatgc gctggagcgc aagggcgccc agctcgagga ggcgaaccgc
     4921 gaactggagg cgttcagcta ttcggtgtcg cacgacctgc gcgcgcccct gcgcggcatc
     4981 gacggctgga gcctggcgct cctcgaggat ttcggcgacg gcctccacga gaaggcccgc
     5041 ggctacctcg ggatcgtgcg ctccgaaacg cagcgcatgg gacaactgat cgacgatctg
     5101 ctgatgctct cgcgcgtggg ccgggcaggc atccggcacg agccgctcga tctcagcgcg
     5161 atcgccacgt cggtggccgc gaggctggag caggccgagc ccgcgcgccg gttcgaattc
     5221 gccatccagc agggcatgcg cgccaacggt gacgaacagc tactccggat cgcgctggcc
     5281 aacctcttcg acaacgcctg caagttcacg cgcccgcgag cggtggcgcg catcgaattc
     5341 ggcgcggaag aggagcagga cccggccacc ggggcacgcc gcgccgtcta tttcgtccgc
     5401 gacaacggcg tgggcttcga catggcgcgc gcgaagaacc tcttcggcgc cttccagcgc
     5461 ctgcacacga cgagcgaatt tccgggcacg ggtatcgggc tcgccacggt caagcgcatc
     5521 attcaccgcc atgggggaac gattagcgcc gaggcgcagg tgggccgcgg cgcgacgttc
     5581 aggttcacgc tcgaggaggc cgcatgaaca tgaagccgat actgctggtg gaggacaacc
     5641 cgagcgacgt cgggctgacc cgccgcgcat tcgagaagag cgccatccgc aacacgctgg
     5701 tggtggccga ggacggccag gaagcgctcg actacctctt cgccgaggga agacacgctg
     5761 gccgggcgcc cggcgaagcg cccatgctgg tcctgctgga cctcaagctg ccccgcgtgg
     5821 acgggctgga agtcctgcgg cgcatgcgtg ccgacccgcg cacgcggcgc attccggtgg
     5881 tgatcctcac ctcgtcgtcg gaggagcagg acatcgccct gggctacgac ctgggcacca
     5941 acagctacat ccgcaagccg gtggatttcc acaacttcgc cgacgtcatc cggcagctcg
     6001 ggatgtactg gctggtgatg aacgagcccc cgcccgatgc ctgaagccgc caggggcgag
     6061 cccctttccc tcctcgtcgt cgaggattcc gccagcgacg ccgggctgat ggtccggcac
     6121 ctcgagcgca acggcttcga cgtggcggac cggcgcgtcg agacggagga cgagttgcgc
     6181 gcagccttgc gcgaggggcc gtgggacgtg gtgctttgcg atttcaacgt tccgggcttc
     6241 gacgccggcg cggcccttgc ggcgctgcac gagaccgggc tggacatccc gttcatcgtg
     6301 gtctcgggcg tcatcgtcga gggagcggcg atcgagctga tgcgcgcggg tgccagcgac
     6361 tacgtgatga aggaaaacct cgcacgcctt gccccggtgg tgcggcgcga ggtcgaggaa
     6421 gcgcgcgggc gtgcggcgag tcgccgggcg caggccgcgc ttgcggcaac cctgcagcgg
     6481 cacgcggcgg ccatcgattc cgccctcgac ggttacgccg cccttgacgg cgggcaccgc
     6541 atcctcgagg tcaacgacgc gctgtccgcc atgacgggcc gctcgcggga ggaactgctg
     6601 caactgcgcg tggatgagct gttcgccgcg ggagccatcg ccgcctcgca ggccttcccg
     6661 tcccgcgaag gcggaggcca cgcgcgtttc gacagccagt ggcggcgcaa ggacggctcg
     6721 acgctcgacg tcgagctttc ggtcacctgc cacggcggcg aggacatcga gatcttcctg
     6781 ttcgcgcacg acatcaccat gcgcaagcag cgcgaagccg aactcggggg cctcacccgc
     6841 gaaagcgaac gggcgcgcct cgcgctgctg ggcgtgctcg aggaccagcg cgagatcgcg
     6901 gaggaactgc gcgagagcga ggagcagttt cgcggcatgc tcgagcacaa tgtctcggcg
     6961 atgttcctga tcgaggacgg caacctcacc tacgccaacc cgcgaacggg cgagatcctg
     7021 ggttacacca gcgacgagat gacgggaaag ccgttcatct ccttcgtcgt cgacgaggac
     7081 cggcagatga tcgcccgggt ggtggagcaa ctgatatccg ggaagctggg caaggccgaa
     7141 cgcaatttca aggcgatccg caaggacggc tcccacgtcg acatcggcgc cagcgcgacc
     7201 cgcgccatgc tgcgcggcaa ggtcatgatc ctcggcgcgg cgcaggacat cggcgagcgc
     7261 aagaaggcgc gcgaggagat cgaccggtac atcgtgcggc tcgaaagctc catgcaggga
     7321 accctcgagg cggtgtcgca catggtggag ctgcgcgatc cgtacacctc gggacacgag
     7381 cgccgggtgg gagacctggc tgccgccatc gccgccgaaa tggggttgcc cgacgcggtg
     7441 atcaagggcc tgcgcctcac gggctacgtg cacgacatcg gcaagatcag catcccggcg
     7501 gagatcctgt ccaagccggg gcggctgacc cccctggagt acgagctgat caagtcgcac
     7561 agccaggcgg gattcgaggt cctcaagggc gtcgacttcc cgtggcccgt cgcggaggcg
     7621 atcctgcagc accacgagcg cgccgacggc agcggttacc cgcgcgggct caagggtgag
     7681 gagatcatcc tcgaggcgcg cgtcatcgcc gtggccgacg tgctggaggc catggggtcg
     7741 caccgcccct atcgtccctc gctcggcctc gaggccgcgc tcgcggagat cgagaagaac
     7801 gcgggccgcc tgtacgacgc gaccgccgcg gccgcctgcc tgaggctctt tcgcgagaag
     7861 ggttattccc tgggcgcctg aagcggcccg ctcaccggaa ggcgccttgc gccggtcatg
     7921 cgggaaccgg cgggcgcttg tcgaagagcg gaaagaaggc gcggtccacc gtcatcaggt
     7981 gccgcgcgac gacatcctgg gcgaggaact cgacgacgga gcccaggctg gcctcgccgg
     8041 cttccgcgcg ctccttcagg tagcccgccc gccggagcag gcccttgtgc gcggccttgt
     8101 gctgctccag gtcggcatag tggcagcggg cgaggatggc ctcctcgtcg gcgaagtgcc
     8161 gctgcacgtg cgcaagcagc acgtcgaggg cctcccggaa ggtcgccggc tcctcctcgc
     8221 cgcgcgctac ggcatcgatg agccggttgg ccagcacgaa gagctgctcg tgttcgccgt
     8281 cgatggtggc gtcgccgcac tcgtacgcct cctgccactc gaggtgcagg gcggacacgc
     8341 cgacttcggc cgcccaggcg tcggaactgc cgcggcgatc cacgaccacg cggttgcgcc
     8401 cgccattctt ggcggcatag agcgtttcgt cgaggcggcg aaaccaggat tgcggatcct
     8461 cgccaccggc gtgctcggcc acgccgaggc tgatggtgac cgtgcccacg ccgcgaaatt
     8521 catgccccgc caccgcggca cggaggtttt ccgccacgcg ctccgcgccg cggtagcccg
     8581 cggaggcgac gagcaccgtg aattcctcgc cgccccagcg gaacaggacg tcggaagcgc
     8641 ggatgcgcga ctggaccagg cgcacgagct cgcgcagcac ggaatcgccc atgccgtggc
     8701 cgaacgtgtc gttgatccgc ttgaagtgat cgatgtcgag gagcaccagg gacaccggct
     8761 gccggctccc ggtgctgcgg gcaatttccg cgtcgatcag gcggtcgaag tgcgagcgat
     8821 tccacgcgcc ggtcaggtgg tccgtggcgg cgaggcgctc gagctgcgag acgcgttcgc
     8881 gaagcccttc catctcgtca tgccgcctcg gcccgcccga ttcgtcgagg atcaggagaa
     8941 tccggtccgg catcgggatg gcccgggcgc ggacctcgac cgcctgctcg aggcccggcg
     9001 atcgcaaggc gacgcgacgc tcgccgccct cgccccgcaa caattcgcga aggctctccg
     9061 ggaccacgcg ggcttcgccg aaacgggacg agaacgccgc attcaccagc acagccgtgc
     9121 cttcgagatt caccagcgcc atcggggcgg gaaactgcac gaaggcctgg aggatatccg
     9181 cgacgccgga cggtactgcc gttcgatgtt cttgttggct catcgggata atctgccccc
     9241 tttaaccgcg cccgcgttga ccttccacaa ggtttcccgc cccgcgcctc cgaaaatggg
     9301 gtcat
//
LOCUS       DASUEV010000117         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_835968, whole genome shotgun sequence.
ACCESSION   DASUEV010000117 DASUEV010000000
VERSION     DASUEV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835968"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..970)
                     /locus_tag="VFV55_03880"
     CDS             complement(290..970)
                     /locus_tag="VFV55_03880"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX4943467.1"
                     /translation="MDPIAHLALATLVFLATHFASSTAVRSSLVESIGERAYLGAYSL
                     VSFVTIGWMAWAYLHAPFLPLWQVPGVKLWPLAVMPFSLVLVAAGVMTKNPSAVGQAA
                     ALKAGDPARGILRVTRHPVMWGIALWAAVHLVARGDVASLVFFGGFLALALAGTALID
                     ARKNDTLGEEWARFAAVTSNVPFSAIVEGRNHFSASEVGASRLAVGLALYAIVVAIHP
                     WLFGVRAY"
     gene            complement(983..2791)
                     /locus_tag="VFV55_03885"
     CDS             complement(983..2791)
                     /locus_tag="VFV55_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943468.1"
                     /translation="MQLGIVALLVAAAGLAATPPACAADPAKVFRYAFEIAESSLDPQ
                     RISDVYSNIVNAAMYDPPLRYDYLARPLKLRPNTLASMPEISADGLTYTLRVKPGIHF
                     ADDPVFNGKKRELVAEDYVYSIKRLMDPKLAAPLLSEVEGIIVGSDEALAKARKANRF
                     DYDAPIEGLKALDRYTFQIKLTQPFYVFIYNLADCRVSCAVAREMVEHYGDDFGSHPV
                     GTGPYRLAFWKRSSKLVFEANPDYREEYFDGEPGPDDAEGQAILAVQRGKRLPMVGKV
                     EVYIIEEVQPRWLAFLNEEHDLAFRVSEEFANVAMPNNRLAPNLRKRGIRMAQVPALD
                     LTFAYFNMEDPVVGGYTPEKVALRRAISLAYNTGDEIAIVRKNQAVPAHTPYSPGVAG
                     YDPDFRTGANEYSIAKSRALLDMFGYLDRDGDGYREMPDGSPLVLRNASTPSARDQQV
                     DELWKRSMDDVGLRMTFKKAKWPDLLKESDAGKLMMWQLGGAASAPDADTWLTSLYGP
                     NAGFKGNRARFRLDAYDRLYEQARVMPDGPQRTRIYQQMTKLVVAYAPWKVNVHRILT
                     DMWYPWVVGYRRPLVQSSNFWKYIDIDLSKRPPSSR"
     gene            2934..>3655
                     /locus_tag="VFV55_03890"
     CDS             2934..>3655
                     /locus_tag="VFV55_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943469.1"
                     /translation="MARIAPIVMFGAAFVAGAATAADPIAIGISIAQSPPGSVVQGTQ
                     VKDGVEIAKDMINAKGGVLGRPIVLIYEDNQGIPEKGRAATEKLITKDKVVAVTGGHQ
                     SSVCLAEIEVAHRYATPYVNTNCWSDDVRKKGYAEAFNPGNYNTRVSMAMAETIAAMG
                     VKNVVAFAENTDYGIGQAKLLAEFIKTKAPAAKFKYETLDRAGKDFTPAVLALRASPP
                     DMVINVMLPPAAYILMNQLYEQG"
BASE COUNT          648 a         1172 c         1240 g          595 t
ORIGIN      
        1 ccttgctcca cgacggcgcg gagcagctcg ggcagaagcc cgagactttc gaaagcctga
       61 ctgatggtgt tctcctgaaa gatcggcctg gaagggcatt gcgcccccga ccggttcagg
      121 cagacaagac gtgcatcatt gtgaatccgc gccgcagggg ctggcggaag ggcttgaggt
      181 gccaaccggt tgaagcgcct cgggaaccct ggatccgcat tctacgcgaa aacccgtatc
      241 ggggacggtt ccggagaacc ggtattgggg acaggtctgg aattcgctct taataggccc
      301 gcacgccaaa gagccagggg tggatggcca cgacgattgc atacagggcc aggccgacgg
      361 cgaggcggct tgcgcccacc tcgctcgccg aaaagtgatt gcgcccctcg acgatcgcgg
      421 aaaagggcac gttggacgtg accgcggcga agcgggccca ctcctcgccc agggtgtcgt
      481 tcttgcgtgc gtcgatgagg gccgtgccgg cgagggccaa tgcgaggaag ccgccgaaga
      541 agaccagcga ggccacgtcg cccctggcaa ccaggtggac cgcggcccac agtgcgatac
      601 cccacatgac cgggtgccgc gtgacgcgaa ggatcccccg ggcagggtca ccggccttga
      661 gcgccgccgc ctggcccacg gccgacgggt tcttcgtcat cacgccggca gccacgagca
      721 cgagcgaaaa cggcatcacc gcgagtggcc acagcttcac gccgggaacc tgccacagcg
      781 gcaggaaggg cgcgtgcagg tacgcccacg ccatccaccc gatcgtgacg aacgacacca
      841 gcgagtaggc gcccaggtag gccctctcgc cgatcgactc caccagggag ctgcgaacag
      901 ccgtgctgga ggcgaagtgc gtggcgagaa aaacgagcgt ggctagcgcg aggtgggcga
      961 ttgggtccat cgggatggca tttcagcgcg aggagggtgg tcgtttcgaa aggtcgatgt
     1021 cgatgtactt ccagaagttg ctgctctgca ccagcgggcg gcggtaaccc acgacccacg
     1081 ggtaccacat gtccgttagg atgcggtgca cgttgacctt ccacggcgcg taggccacga
     1141 ccagcttcgt catctgctgg tagatcctcg tgcgctgcgg cccgtcgggc atcacgcgcg
     1201 cctgctcgta gaggcgatcg taggcgtcca ggcggaagcg cgcccggttc cccttgaagc
     1261 cggcattggg gccgtagagc gaggtaagcc aggtgtcggc gtccggcgcg gacgcggccc
     1321 cgcccagctg ccacatcatc agcttgcccg cgtcggattc cttgagaagg tcgggccact
     1381 tcgccttctt gaacgtcatc cgcaggccca cgtcgtccat gctgcgcttc cacagctcgt
     1441 cgacctgctg gtcgcgggca ctgggcgtcg atgcgttgcg cagcaccagc gggctgccgt
     1501 cgggcatttc gcggtagccg tccccgtcgc ggtcgagata gccgaacatg tcgagcaagg
     1561 cgcgcgactt cgcgatgctg tactcgttgg cgccggtgcg gaaatccggg tcataacccg
     1621 ccacgccggg gctgtagggc gtgtgcgcgg gcaccgcctg gttcttgcgc acgatggcga
     1681 tctcgtctcc ggtgttgtag gcgagcgaga tggcgcggcg caacgccacc ttttccggcg
     1741 tgtagccgcc gacgacggga tcctccatgt tgaagtacgc gaaggtcagg tcgagggcgg
     1801 gcacctgggc catgcggatg ccgcgcttgc gcaggttcgg ggccagcctg ttgttcggca
     1861 tggccacgtt ggcgaattcc tcggagaccc ggaatgcgag gtcgtgctcc tcgttcagga
     1921 aggcgagcca gcgcggttgc acctcctcga tgatgtagac ctccaccttg cccaccatcg
     1981 gcagtcgctt gccgcgctgc accgcgagaa tggcttgccc ctccgcatcg tccgggcccg
     2041 gctcgccatc gaagtactcc tcgcggtagt ccgggttggc ctcgaagacg agcttggacg
     2101 agcgcttcca gaaggcgagg cggtagggcc ccgtgccgac ggggtgcgag ccgaagtcgt
     2161 cgccgtagtg ctccaccatc tcgcgcgcca cggcgcagga gacgcggcaa tcggcgaggt
     2221 tgtagatgaa gacgtagaag ggctgcgtga gcttgatctg gaaggtgtag cggtccagcg
     2281 ccttcagccc ctcgatgggc gcgtcgtagt cgaacctgtt ggcctttcgc gccttcgcga
     2341 gcgcctcgtc gctgccgacg atgatgccct ccacctcgga aagcagcggt gccgcgagct
     2401 tcgggtccat caaccgcttg atggaataca cgtagtcctc ggccaccagc tcgcgcttct
     2461 tcccgttgaa cacggggtcg tccgcgaagt ggatgccggg cttcacgcgc agggtgtagg
     2521 tcaggccgtc ggcgctgatc tccggcatcg acgcgagcgt gttgggcctg agcttgagcg
     2581 gccgcgcgag gtagtcgtag cgaagcggcg ggtcgtacat cgcggcgttg acgatgttcg
     2641 aatagacgtc gctgatcctc tgcgggtcga ggctcgactc ggcaatctcg aaggcgtaac
     2701 ggaaaacctt ggcggggtcc gcggcacagg cgggcggcgt ggccgcgagg cccgcggccg
     2761 ccacgaggag cgcgacgatc ccgagttgca ttgtccttcg cctgtcgagt gccgtgagtc
     2821 tctgctccgg gtagtatact cagccggtcg gtcaccgaag aggccgatgc ggtaaattgc
     2881 gcggccacgc attgcgcatc gccgctccaa aattcctgag gaggagagta tccatggcga
     2941 gaatcgcccc gattgtcatg ttcggcgcag ccttcgtggc cggcgccgcc accgctgcag
     3001 acccgatcgc gatcggcatc agcatcgcgc agtccccgcc cggatccgtc gtccagggca
     3061 cccaggtgaa ggacggcgtg gagatcgcga aggacatgat caacgccaag ggcggcgtgc
     3121 tggggcgtcc catcgtgctc atctacgagg acaatcaggg catcccggaa aagggccgcg
     3181 ccgccaccga gaagctgatc acgaaggaca aggtcgtcgc cgtcaccggc gggcaccaga
     3241 gctccgtgtg cctcgccgag atcgaggtgg cgcaccgcta cgccacgcct tacgtgaaca
     3301 ccaactgctg gtccgacgac gtgcgcaaga agggctatgc ggaagcgttc aaccccggca
     3361 actacaacac gcgcgtctcc atggcgatgg ccgagacgat cgcggcgatg ggcgtgaaga
     3421 acgtcgtcgc cttcgccgag aacacggact acggcatcgg gcaggcaaag ctcctcgcgg
     3481 aattcatcaa gaccaaggcg ccggccgcca agttcaagta cgagacgctg gatcgcgccg
     3541 ggaaggactt cacgccggcc gtgctcgccc tgcgcgccag cccgcccgac atggtgatca
     3601 acgtcatgtt gccgccggcc gcctacatcc tcatgaacca gctctacgag caggg
//
LOCUS       DASUEV010000118         6386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1064929, whole genome shotgun sequence.
ACCESSION   DASUEV010000118 DASUEV010000000
VERSION     DASUEV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064929"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VFV55_03895"
     CDS             complement(<1..115)
                     /locus_tag="VFV55_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4943470.1"
                     /translation="MKKVQQGFTLIELMIVVAIIGILAAVAIPAYQDYIVRS"
     gene            complement(445..2094)
                     /locus_tag="VFV55_03900"
     CDS             complement(445..2094)
                     /locus_tag="VFV55_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4943471.1"
                     /translation="MAPTLANLQHALATHFLSKLEYLNAIGIALSQERDTNRLLETIL
                     VAAKNLTRADGGTLYRLADDKLQFEILRNDSLSIEMGGTSGNPVPFYPIPLHDKDGNP
                     NRTMVAAYAALNDRTVNIADAYTEKGFDFSGTRNFDKRTGYRSTSFLTVPMKNHEGEI
                     IGVLQLLNAIDAKTGGVTVFSDEDQRLAESLASQAAIALTNRLLIQQLEVLFESLIEL
                     INTAIDDKSPYTGGHCKRVPTLTMMLAEAANAATEGPLAQFRMTDKDRYELKIAGLLH
                     DCGKITTPVHVVDKATKLQTIYDRIGLLDTRFEALKREAEIAMLRAALAAREAQDASS
                     EQRAREDFEAKVRQYDDDRAFLRRTNVGGERMSPEDQVRVSRIAQYTWTGPRGAPERF
                     LTKDEEANLNIPYGTLNPQEREIINHHIMATIKMLEALPWPRHLLNVPEYAGGHHERM
                     DGKGYPRGLTRAQMSVQARIMGIADIFEALTARDRPYKPGKTLSESLAILGKFKENGH
                     IDPDLFDIFVKDQVYLRYAREFLDPEQIDEVDVAKIPGYPG"
     gene            complement(2127..4517)
                     /locus_tag="VFV55_03905"
     CDS             complement(2127..4517)
                     /locus_tag="VFV55_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX4943472.1"
                     /translation="MSAVLEPKVPQASAGAADVTVKLAFSKKIQAVTNRIHSTNNVDE
                     IILEVSRDICQLFEADRMTIYTIGEDGQSIVSKVKTGLNSFKDIRLPVSESSLAGFCA
                     LHKRHMNIKDVYDDAELKQFSPNLAFLKAVDQRTGYRSKQMLIAPILGGESGSDLMGV
                     LQLINSLSGQPFSALHDEGVMELAKTLAIAFRVRQQMPGQIASKFEYLVIDSIIQAGE
                     LDLATKAARRKNRNVEEILIDEFQVKPAAIGAALAKYFSVEYEPHKADRIKPIDLLKN
                     LKREYCEANQWIPLEDSKAGIVVMATDPERVRNSRMVDNVFPRAKIIYKVTTQRDFAQ
                     VLTLYFGGAGESGMGTESIGDMLSNLEEDEEPGAIAADDVSAAADNELVKLVNKIIVD
                     AYNQGASDIHVEPLPGKGKTGVRFRKDGSLSTYIEVPAAYRQAMITRLKIMCDLDISE
                     KRKPQDGKIKFKKFGPLDIELRVATIPTAGGVEDVVMRILAAGEPIPLDKLGVLPTNM
                     ERLKSCVSKPYGLFFVCGPTGSGKTTTLHSVLGFLNTVDTKIWTAEDPVEITQKGLRQ
                     CQVNPKAGFTFATAMKAFLRADPDIIMVGEMRDKDTTSIGIEASLTGHLVFATLHTNS
                     APESIIRLLDMGMDPFNFADALLGILAQRLAKRLCKCKEAYTPEQDEIQHFITEYCEE
                     LRNTEEFKQDANAGYKSVFDRFQKDYGFGKAGFTMYKAKGCDICGGSGYKGRVGLHEL
                     MVGTDAVKKLIQEHARVAQLFAQALSDGMLTLKMDGMEKVLQGITDMKQVRTVCIK"
     gene            complement(4569..5333)
                     /locus_tag="VFV55_03910"
     CDS             complement(4569..5333)
                     /locus_tag="VFV55_03910"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX4943473.1"
                     /translation="MRIRVLGCSGGIGGSLRTTAFLVDEDILVDAGTGVGDLSLESLA
                     KIDHIFVTHSHLDHVTSIPLLVDTVCWMRGRPIVVYGIEGTLDILRAHVFNWKVWPDF
                     TQIPDGDNPFMVYREIAVGETVEIGGRRITAIPANHTVPAVGYLLDSGRASLAYSGDT
                     TANAALWEVVNGARNLRYLIIETAFSNKERDIAVASKHLCPEMLARELDRMRARPEVF
                     ITHLKPGEGALTMKEVSTAAGRWEPRRLENNQEFTI"
     gene            complement(5419..6249)
                     /locus_tag="VFV55_03915"
     CDS             complement(5419..6249)
                     /locus_tag="VFV55_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX4943474.1"
                     /translation="MAKLILSVDGQVLKEHTLSKERTLIGRKPHNDIQIDNLAVSGEH
                     AAIITILNDSFIEDLGSTNGTMVNGKPIKKHFLQANDVIEIGKHKLKYFNDAPSQASA
                     ADFEKTMIIRSPSRPQPAAPPPAPGPAAAPAAERMTSDTLTGMKAMTPEVVRAVPPPP
                     VSVAPAAPAPPAAPVAPAAPVAHAAHAPLPDAAIQVLSGAAAGRTLDLTKNLTTIGKP
                     GLQVAVITKRPNGYFITHVEGASYPALNGTSLGPQAHTLGDHDMIEIAGVKMEFFFKR
                     "
BASE COUNT          936 a         2008 c         2170 g         1272 t
ORIGIN      
        1 tggaacgaac gatgtagtcc tggtaggccg gaatcgcgac agccgcgaga atgccgatga
       61 tcgcgaccac gatcatcagt tcgatcagcg taaagccctg ttggaccttt ttcattggtg
      121 ctcctgtgta agtaatgggg ggaaacgctc tccacaccgt agtgtgcaag cgacaagtac
      181 gcaaccgccg tgccacgagc tgccgccaac taccgttgtc gcccgccggg ccggaaatcg
      241 gaatcggcgg cactttccct tgccccatca atgacttccg gaattcggcc tggcgccgcc
      301 ccgcccctgg tccgaacggc ttcgtccctt cccggcatgg gccggcccgg cgcctgcctc
      361 atttggaccc attagtgcct caattcgtca cctcgtgggc cgtcatgcca gatcggtgcc
      421 cacgaggggg aaagaaacga acggctatcc cggatagccg gggatcttcg cgacatccac
      481 ctcgtcgatc tgctcgggat cgaggaactc gcgtgcataa cgcaggtaga cctggtcctt
      541 cacgaagatg tcgaagaggt ccgggtcgat gtggccgttc tccttgaact tcccgagtat
      601 cgcgagggac tcggagagcg tcttgcccgg cttgtaggga cggtccctgg cggtgagcgc
      661 ctcgaagatg tcggcgatgc ccatgatgcg ggcctgcacg ctcatctgcg cgcgcgtgag
      721 gcccctgggg tagccctttc cgtccatgcg ctcgtggtgg cccccggcgt actcgggcac
      781 gttcaggaga tggcgcggcc atggcagcgc ctcgagcatc ttgatcgtgg ccatgatgtg
      841 gtggttgatg atctcgcgtt cctgcgggtt gagcgtcccg taggggatgt tgaggttcgc
      901 ctcctcgtcc ttggtgagga agcgctccgg cgccccgcgc gggcccgtcc acgtgtactg
      961 cgcgatccgc gacacgcgca cctggtcctc ggggctcatg cgctcgccgc cgacgttggt
     1021 gcggcgcagg aacgcccggt cgtcgtcgta ctggcgcacc ttcgcctcga agtcctctcg
     1081 cgcgcgctgc tccgaggagg catcctgcgc ctcccgcgcc gccagcgccg cccgcagcat
     1141 ggcgatctcg gcctcgcgct tgagggcctc gaagcgcgtg tcgaggaggc cgatgcggtc
     1201 gtagatggtc tgcagcttcg tggccttgtc cacgacgtgc accggcgtcg tgatcttgcc
     1261 gcagtcgtgc aggagcccgg cgatcttgag ctcgtagcgg tccttgtccg tcatgcggaa
     1321 ctgcgcgagc ggcccttccg tcgccgcgtt cgcggcttcg gcgagcatca tggtcagcgt
     1381 gggaacgcgc ttgcaatgcc cgccggtgta ggggctcttg tcgtcgatcg cggtgttgat
     1441 gagctcgatg agggactcga acaggacctc gagctgctgg atgaggagcc tgttggtgag
     1501 cgcgatcgcc gcctgggagg cgagcgactc ggccaggcgc tggtcctcgt cgctgaacac
     1561 cgtgacgccg ccggtcttcg cgtcgatggc gttgagcagt tgcagcaccc cgatgatctc
     1621 gccctcgtgg ttcttcatcg gcacggtaag gaacgaggtc gagcggtagc cggtgcgctt
     1681 gtcgaagttg cgcgttcccg agaagtcgaa acccttctcc gtataggcgt cggcgatgtt
     1741 caccgtgcgg tcgttgagcg ccgcgtaggc cgccaccatc gtgcggttgg gattgccgtc
     1801 cttgtcgtgc agggggatcg ggtagaacgg gacggggttc cccgacgtgc cgcccatctc
     1861 gatcgacagg ctgtcgttcc tcaggatctc gaactgcagc ttgtcgtcgg ccagccggta
     1921 gagggtgccg ccgtcggcgc gcgtgaggtt cttggccgcg acgaggatgg tctccagcag
     1981 gcggttggtg tcgcgctcct gggaaagggc gatgccgatc gcgttgaggt actcgagctt
     2041 cgaaaggaag tgcgtcgcga gcgcatgctg caggttcgcc agcgtcggcg ccacggcttg
     2101 cgttggcgct tgcccgccct gcagcgtcat ttgatgcaga ccgtccgcac ctgcttcatg
     2161 tcggtgatgc cctgcagcac cttctccatg ccgtccatct tgagggtgag catgccgtcc
     2221 gagagcgcct gcgcgaagag ctgcgccacg cgggcgtgtt cctggatcag cttcttcacc
     2281 gcatccgtgc cgaccatgag ctcgtgcagg cccacgcgcc ccttgtagcc ggagccgccg
     2341 cagatgtcgc atcccttggc cttgtacatg gtgaagccgg ccttgccgaa gccgtagtcc
     2401 ttctggaagc ggtcgaagac gctcttgtag ccggcattcg cgtcctgctt gaattcctcg
     2461 gtgttgcgca gttcctcgca gtactcggtg atgaagtgct ggatctcgtc ctgctcgggc
     2521 gtgtacgcct ccttgcactt gcacaggcgc ttggccaggc gctgcgcgag gatgcccagc
     2581 agggcgtcgg cgaagttgaa cgggtccatg cccatgtcga gcaggcggat gatcgattcc
     2641 ggcgccgagt tcgtgtgcag cgtcgcgaac accaggtggc ccgtgagcga ggcctcgatg
     2701 ccgatcgagg tggtgtcctt gtcgcgcatc tcccccacca tgatgatgtc cgggtcggcg
     2761 cgcaggaacg ccttcatcgc ggtggcgaag gtgaaccccg ccttggggtt gacctggcac
     2821 tggcgaaggc ccttctgcgt gatttccacg gggtcctcgg cggtccagat cttggtgtcg
     2881 acggtgttga ggaagccgag gacggaatgc agcgtcgtcg tcttgcccga gcccgtcggt
     2941 ccgcagacga agaacaggcc gtacggcttc gacacgcagc tcttcaggcg ctccatgttg
     3001 gtgggcagca cgccgagctt gtccagcggg atcggctcgc ccgccgccag gatgcgcatc
     3061 acgacatcct cgacgccgcc ggccgtgggg atggtcgcca cgcgcagctc gatgtcgagt
     3121 ggaccgaact tcttgaactt tatcttgccg tcctgcggct tgcgcttctc cgagatgtcg
     3181 aggtcgcaca tgatcttcag gcgcgtgatc atggcctggc gataggccgc gggaacctcg
     3241 atgtaggtgg agagcgagcc gtccttgcgg aagcgaacgc ccgtctttcc cttgcccggc
     3301 agaggctcga cgtggatgtc cgaggcaccc tggttgtagg cgtcgacgat gatcttgttg
     3361 accagcttca ccagctcgtt gtccgcggcc gcggagacgt cgtccgcggc gatcgcgccg
     3421 ggctcctcgt cctcctcgag attcgagagc atgtcgccga tcgactcggt gcccatgccg
     3481 gactcgccgg cgccgccgaa gtagagggtg aggacctggg cgaagtcgcg ctgcgtggtg
     3541 accttgtaga tgatcttcgc gcgcgggaac acgttgtcca ccatgcgcga gttgcgcacg
     3601 cgctccgggt cggtggccat caccacgatc cccgccttgg aatcctcgag cgggatccac
     3661 tggttggcct cgcagtactc gcgcttgagg ttcttcagca ggtcgatggg cttgatgcgg
     3721 tcggccttgt gcggctcgta ctcgacgctg aagtacttgg cgagcgcggc gccgatggcg
     3781 gccggcttca cctggaactc gtcgatgagg atctcctcga cgttcctgtt cttgcgccgc
     3841 gcggccttgg tggcgaggtc gagctcgccg gcctggatga tgctgtcgat gacgaggtac
     3901 tcgaacttgg aggcgatctg ccccggcatc tgctggcgca cgcggaaggc gatcgcgagc
     3961 gtcttggcga gctccatcac gccctcgtcg tgcagcgcgc tgaacggctg acccgacagg
     4021 ctgttgatga gctgcagcac ccccatgagg tccgagcccg actcgccgcc caggatcggc
     4081 gcgatgagca tctgcttcga gcggtatccg gtgcgctggt ccaccgcctt caggaacgcg
     4141 aggttcggcg agaactgctt gagctcggcg tcgtcgtaga cgtccttgat gttcatgtgg
     4201 cgcttgtgca gcgcgcagaa gccggcgagc gacgattccg agaccgggag cctgatgtcc
     4261 ttgaaggagt tgagccccgt cttcaccttc gagacgatgg actggccatc ctcgccgatg
     4321 gtgtagatcg tcatgcggtc cgcctcgaag agctggcaga tgtcgcgcga gacctcgagg
     4381 atgatctcgt cgacgttgtt cgtggagtgg atccggttcg tgaccgcctg gatcttcttc
     4441 gagaaggcca gcttgaccgt gacgtccgcc gcgccggccg aagcctgcgg caccttgggt
     4501 tcgaggactg cgctcatgtg tgtacctgcc gatcggttgt tgccgcctgt cgggagatcc
     4561 cgcgaccgct agatcgtgaa ttcctggttg ttctcgagcc tgcgcggttc ccagcgcccg
     4621 gcggccgtgg acacctcctt cattgtaagc gccccttcgc ccggcttgag gtgggtaatg
     4681 aacacctcgg gccgtgcccg catccggtcc agttccctcg ccagcatctc cgggcagaga
     4741 tgcttcgacg ccaccgcgat gtcgcgctcc ttgttggaga aggccgtctc gatgatgagg
     4801 tagcgcaggt tcctggcccc gtttaccacc tcccacaggg cggcgttcgc ggtcgtgtcg
     4861 ccggaatagg cgaggctcgc ccggccgctg tcgagaagat accccaccgc gggcaccgtg
     4921 tggttggccg ggatggcggt tatgcgccgc ccgccgatct ccaccgtctc gccgaccgcg
     4981 atctcccggt acaccatgaa ggggttgtcg ccgtcgggta tctgggtgaa gtcgggccag
     5041 accttccagt tgaagacgtg ggcgcgcagg atgtcgaggg tcccctcgat gccgtagacg
     5101 acgatcgggc ggccgcgcat ccagcaaacc gtgtccacga ggagcgggat gctggtcacg
     5161 tggtcgaggt gcgaatgggt cacgaagatg tggtcgatct tcgccaggct ttccagagac
     5221 aggtcgccca cgcccgttcc ggcatcgacg aggatgtctt catcgacgag gaatgcggtc
     5281 gtccgcaggc ttccgccgat gccgccgctg caccccagaa ccctgatccg catcttctga
     5341 cctcggcccg tggacacggg cttgtttgcg gcccgcgagc caccgcgccg gccgggggcg
     5401 gccgccgggg ccggtagctc agcgcttgaa gaagaactcc atcttcaccc cagcgatctc
     5461 gatcatgtcg tggtcgccca gggtgtgggc ctgggggccg agcgaggtgc cattgagggc
     5521 cggatagctc gctccctcga catgggtgat gaagtagccg ttcggccgct tggtgatgac
     5581 cgccacctgc aggcccggct tgccgatggt ggtgaggttc ttcgtgaggt cgagcgtgcg
     5641 ccccgcagcg gcaccggaca gcacctggat cgcggcatcc ggaaggggag cgtgcgcggc
     5701 gtgcgctact ggcgcggcag gcgctacagg cgccgccggc ggggcaggcg cggccggcgc
     5761 cacggacacg ggcggcggcg ggacggcccg gaccacctcc ggcgtcatcg ccttcatgcc
     5821 ggtgagcgta tcgctggtca tccgctccgc ggccggtgcc gcggcaggac cgggggccgg
     5881 cggcggcgcg gcgggctgcg gcctggaggg gctgcggatg atcatcgtct tctcgaagtc
     5941 ggccgccgag gcctgcgagg gggcgtcatt gaagtacttg agcttgtgct tgccgatctc
     6001 gatgacgtcg ttggcctgca ggaaatgctt cttgatgggc ttgccgttga ccatggtgcc
     6061 attggtgctg cccaggtcct cgatgaacga gtcgttcagg atcgtgatga tcgccgcatg
     6121 ctcgccgctg accgcgaggt tgtcgatctg gatgtcgttg tggggctttc tcccgatcag
     6181 ggtgcgctcc ttggagagcg tgtgctcctt gagcacctgt ccatcgacac tcaatatcag
     6241 ctttgccatg gtcggattcc ttgcggatta cctgaatatt gccttgaagc gtgcaagcca
     6301 gccacgcgcc acggcgaagg gttcggcgac gccgatgagc atcgccgaga tgttgtcgcg
     6361 gccgccattg tcgttggccg cctgga
//
LOCUS       DASUEV010000119        13437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1065641, whole genome shotgun sequence.
ACCESSION   DASUEV010000119 DASUEV010000000
VERSION     DASUEV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065641"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..2129)
                     /locus_tag="VFV55_03920"
     CDS             complement(84..2129)
                     /locus_tag="VFV55_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX4943475.1"
                     /translation="MDLKHWKLEMDAQSLAWLWFDRAGTATNTFSTEVLQELDRIADH
                     LAAMPPKGLAILSAKENGFAAGADIDEFTTISSAEQAMAFTTLGNAVFDRIAALPFPT
                     LAMIHGFCMGGGTELALACRYRMADDGPKTRMALPEVLIGIVPGWGGAKRMPALIGAA
                     NALDLMLTGRGVDGRRAKKLGLVDAVTQKRHFGNAVRMFLASPPPPHKPPLSAAITDW
                     PVVRSFVASMSRKKVAEKARQDHYPAPYAIIDLWKDFGGDVRNIPVGHPASMASLFAH
                     STTRNLIRLFKLQDRMKAFGKRGSVPIDARDRGGEPIRHLHVVGAGAMGGDIAAWCAL
                     KGITVTLQDLGPERIAPAIQRAHALFKKRLKQPHLVRAAFDRLIPDVKGDGVPKADLV
                     IEAIVENAEVKRKLFAGIEPRMKEGAILASNTSSIPLQELSGVLERPERLVGLHFFNP
                     VAMMMLVEIVEGPQTSPEVMQAGQAFARQIDKLPLPCKSAPGFLVNRVLSPYLMEAML
                     MLDEGVPGEAIDAAAKDFGMPMGPIELADMVGLDICWAVGQELAKPGTPIPKKLQASI
                     EAKNLGKKTGQGFYKWVKGKPRKASGSGSVDLKPIADRMVVAALNEAVACLREKIVTD
                     ADLCDAGVIFGTGFAPHRGGPISAIRERGKESWLGTMAELRAKHGDRFVPDTGWDTI"
     gene            complement(2147..3430)
                     /locus_tag="VFV55_03925"
     CDS             complement(2147..3430)
                     /locus_tag="VFV55_03925"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX4943476.1"
                     /translation="MAARRVHVVDGARTPFLKAKKGLGPFTGADLAVAAGRPLVTRQP
                     FDATAFDEVIVGAALPSADEANIGRVVSLRLGCGEKVPAWTVMRNCASGMQAIDTACL
                     NIQAGRSSLVLAGGTDAMSHAPLLFGPAMVNWLADWYAAKSFGQKAATAAKFRLANLA
                     PVIAILRGLTDPVVKLSMGSTAEIVATRFGITRQMMDEFAVESHRRLAFAQDNGHLAE
                     IEPIYGPDGTVYSTDDGLRRDSAVEKLARLKPVFDRTYGNVTPGNSSQITDGAAMLIL
                     AAEEVVQEYGLTTLGRVVDANWGALDPAEMGLGPVHAITPMLQRNHLGWDDIDCVEVN
                     EAFAAQVLGCLAAWESDEYCGTHFGLDRAFGRLDRGKLNVDGGAIAMGHPIGASGARV
                     VLHALKVLERTGGRRAVASLCIGGGQGGAMLLERP"
     gene            complement(3406..5868)
                     /locus_tag="VFV55_03930"
     CDS             complement(3406..5868)
                     /locus_tag="VFV55_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4943477.1"
                     /translation="MLPIILSVLAAIAVAWALAYHRANAVAWSAALAVGAACLAGFTA
                     APAGVLAAAWVVVALIAALAVVKPIRRAIVTGPLFGVFKRVLPQVSQTEQEALDAGSV
                     WWDADLFSGSPGWTKLLAYPAPKLTAEEQAFVDGPVEELCGMLDEWVINHERLDLSPE
                     AWKFIRDKGFLGIIIPKSYGGLGFSAFAHSEIVTKISTRSGAAAVTVMVPNSLGPAEL
                     LIHYGTDEQKNHYLPRLARGQEIPCFALTNPDAGSDAASIPDFGIVCKGLHEGREVLG
                     IRLTWEKRYITLAPVATLLGLAFKLYDPEKLLGAQEDVGITLALVPTSHAGVVVGRRH
                     DPLGCAFMNGPTTGKDVFIPLDWVIGGKAQAGTGWRMLMECLAAGRSISLPSMSMAAG
                     KIGTRVTGAYARIRSQFKTPIGRFEGIEEPLARIGGSTYLMDGMRRMTMAALDMGEKP
                     SVISAICKYHMTERMRSILNDAMDVHGGKGICMGPNNYLGRAYQTVPIAITVEGANIL
                     TRSLIIFGQGAIRCHPWVLKEIRAAGADDLVAFDRALWGHVAFTASNGARALWLGLTG
                     GKGISVPGSADTRRYLQILTRFSSAFALLADMSMFVIGGSLKRKEMLSARLGDILAHL
                     YMMSATVKRFHDDGCPKEDLPLLTWAMYDSSFKLQVAMDGVLANFPNRPIAWVLRHLA
                     FPKGLTLTAPHDKWASRVARLLIEPGAVRDRLTAGIYLPRKEDDAIGRLEFALEAVIK
                     AEPIEAKIRAAQKEGKLPQRTLAERRDAAVKSGVITLAEFDHLSRTDRLRRSVIMVDD
                     FPTDLSRKSNQEKAPWLRAASM"
     gene            complement(5939..6658)
                     /locus_tag="VFV55_03935"
     CDS             complement(5939..6658)
                     /locus_tag="VFV55_03935"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4943478.1"
                     /translation="MPAQHPARRTLAMDALSQDTKSRILDAAESLFTEHGFEATSLRQ
                     LTSAAGVNLAAVNYHFGTKEELFQAVLTRRLDPMNQERIDLLARYEREAGGRPLTCEK
                     ILSAMLIPALRLSRDERRGGKDFLRVLGRAYADPAPFIRTFLSAQYAEMIARFKDAFQ
                     KALPHLTKQELTWRLHFVMGALSYTLAGTDALKLMAQVIPHDKDNDEMLLQRLAPFLA
                     AGLKAPLGDAKKLELASGDWK"
     gene            6806..7594
                     /locus_tag="VFV55_03940"
     CDS             6806..7594
                     /locus_tag="VFV55_03940"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX4943479.1"
                     /translation="MHRVLLLSVALAAAAIPRPADAILVRADRDDAEYRELATRYPSA
                     VGLGAAAGTGVLIAPRWMLTTAHRAAALRAMKGPLLIAGRPHEIQDVLVHPDWKPGGE
                     SDIALIFLRTAADGVEPTPIYRLPDEEGQAARIVGVGETGRIGEKATKPDGLARAAIN
                     TVDRVTPRTLGMRLKGPEDASDLQGALAPGDGGAPAFFEAGGRIFVAGIASTPEGANA
                     GGHARNVGDWEVYARVSAFAAWIDDTMSKVAVEEAARETAKPRR"
     gene            complement(7599..8879)
                     /locus_tag="VFV55_03945"
     CDS             complement(7599..8879)
                     /locus_tag="VFV55_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX4943480.1"
                     /translation="MADREFGFETLALHAGQIPDAQTGARAAPIYQTTSFVFDSADHA
                     ASLFNLQTFGNVYTRLSNPTTAMFEERMAALEGGRAAVATASGQAAEMVALLNICQAG
                     DHIVSSSKLYGGTHTMFAVNFPKLGIEATLVDPDDPENFRRALRPNTKVIFSETLGNP
                     AINMVDLAAVGAIAREAGVPFMVDNTAASPYLCQPFRHGADIVVHSATKFIGGHGTTM
                     GGVVVESGKFPWDNGKFPSMTEPSAGYHGVRFYETFGDFGFTMKARMEILRVFGPAIS
                     PFNAWLLLQGLETLAVRMDRHCANTMGVANFLRDHRKVAWVNYPGLAGNKYHALTKRY
                     MPKGAGALLSFGIRGGYEAGVKFIEGLQFVSHLANIGDAKSLVIHPASTTHRQIPEAD
                     LVKAGVPPDMVRLSIGLETLDDILWDLDQALSRA"
     gene            complement(8883..9878)
                     /locus_tag="VFV55_03950"
     CDS             complement(8883..9878)
                     /locus_tag="VFV55_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX4943481.1"
                     /translation="MFWKVLVAVIVAVAAAFLTQSVGITVATFLIAMIVVLIIEGIRV
                     VPQQQAWVIERLGKFHEVLPPGLNVIVPFIDRVAYRHSLKEVPLDIPEQVCITKDNTQ
                     LAVDGIIYYQVSDPKLASYGSSDYVLAISQLAQTALRSEVGKMELDKTFESRSEINHM
                     VVSVLDDAGRTWGIKVLRYEIKSLTPPEAILRSMQAQITAEREKRALIAKSEGQRQQE
                     INIADGAKQAKVLQSEGDKTAAINKAQGEALALTTVAEATANAVRQVAAAIGAEGGML
                     AANLKVAEKYVEAFANLAKTGNTLIVPANLTDVSTFVSTAMTVLDRTRASSEVPK"
     gene            complement(9904..10338)
                     /locus_tag="VFV55_03955"
     CDS             complement(9904..10338)
                     /locus_tag="VFV55_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX4943482.1"
                     /translation="MEPYLAWIIAGFVLVIIELLSGTFYLLVIGVGAFAGAAVAWAGG
                     GYLGQAIGACAVALVGTWFVNRWHRTRQPGSAKDNFLDLGQPVVLESWVDASSGTARV
                     KYRGTTWDARVAAGAHPEPGATLVINGQDGSTLLVGAAPTSK"
     gene            complement(10440..10943)
                     /locus_tag="VFV55_03960"
     CDS             complement(10440..10943)
                     /locus_tag="VFV55_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX4943483.1"
                     /translation="MTGRILERLSVSHGTMDDIAKLRRILRDNRTIAVVGLSANWYRP
                     SYFAAKYMQDHGYRIFPVNPMYDSVLGEKCYKSLADIPEKVDLVDCFRRSEEIAALAD
                     EAIAIGAKVLWMQLGVRSEEARRKAEAAGLEVVEDRCLKIEHGRLFGGLGWAGVNTKV
                     ISAKRPA"
     gene            10942..12723
                     /locus_tag="VFV55_03965"
     CDS             10942..12723
                     /locus_tag="VFV55_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943484.1"
                     /translation="MRQSLIAFAVAVAVCMPARASNHDTVSPPIGAGPFKVACSNIAQ
                     DEALIAALGSTPAEIWEGRPRDGQARYISQVLAAPGTALQFEAPVPDIRDIYPRLAGE
                     NVHYVAIVCHPTPQANADADYVLPGTSDIVPHMQLPGASPRLIGLAEYAATYGIAVDP
                     AMPSSAPARLPLIVFSHGLGGSPVSPGYVDAMVDLASHGFMVAAVFHGDSRFSRIRVE
                     DLGQFVTLLLNFDGFVEMELLRPVSLKSLLDTLLQHAGFAPGIDAERIGGFGASLGGQ
                     AVANLLGARLTTSLRLACRDTVTDARVKAAVGLVPYAGQTFLPAFCDDQGGAVAVKRP
                     YLALSGTADTTAPIAMVEQAVNRFQGSRYLVELQGVQHEYLPEYRGDVMTWTVTFLNA
                     YLGVAADPGAIARLIRLKSVTGGPQDALRVDAHIPFPPGPEEALVLEFYNDPLAHYFI
                     AAGADEIASILGGGAGPGWQLTGHAFKAYPRLPADAFTTAVPVCRFYGALAGGPNSHF
                     FTADAPECDLVKRSGGWYYEGTGFYITPVGADGRCPSGYLQVLRAYNQGFPRNDSNHR
                     FTTSDSTWREMERHGWVLEGAVMCARA"
     gene            complement(12704..>13437)
                     /locus_tag="VFV55_03970"
     CDS             complement(12704..>13437)
                     /locus_tag="VFV55_03970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4943485.1"
                     /translation="RTRDGTFYYAMEYLDGLSLEELVQKEGRLPVPRAVHALRQVCNS
                     LKEAHERGLVHRDVKPHNLMLCHHGGEFDVVKVLDFGLVKELENVHTRDITQFARVLG
                     TPLYMAPERLRNPTDADARADIYALGAVAFFLLTGRRVFETQSDHDLVYQVLNVAPPS
                     IADRGAQDVPAILEALVARCLAKDREERPRSIAEVAAVLAEVARERPWREEEARDWWA
                     NYAAAREKATGAGDVAPGVVRPGRT"
BASE COUNT         2050 a         4607 c         4543 g         2237 t
ORIGIN      
        1 gcctccacgg atccagggcc gcaatcattt caccgcccat ctgtgttcat ctgtggttcc
       61 aacgcctttg ggtcactgaa ctctcatatc gtgtcccaac ccgtatccgg cacgaaccgg
      121 tccccatgct tcgcccgcag ctccgccatc gtcccgagcc acgattcctt gccccgttcc
      181 cttatcgcgg agatcggccc gccgcgatgc ggcgcgaatc ccgtcccgaa gatcaccccc
      241 gcatcgcaca ggtccgcgtc ggtcacgatc ttctcgcgca ggcacgccac cgcctcgttg
      301 agcgccgcga ccaccatccg gtccgcgatc ggcttcaggt ccaccgagcc gctcccgctc
      361 gccttccgcg gttttccctt gacccacttg tagaagccct gcccggtctt cttgcccagg
      421 ttcttcgcct cgatgctggc ctggagcttc ttcgggatcg gcgtcccggg tttcgccagc
      481 tcctgtccca ccgcccagca gatgtcgagg ccgaccatgt ccgcgagctc gatcggcccc
      541 atgggcatgc cgaaatcctt cgccgcagcg tcgatcgcct cgcccggaac gccctcgtcc
      601 agcatgagca tcgcttccat gaggtagggc gagagcacgc ggttcaccag gaagccgggc
      661 gcgctcttgc agggcagcgg aagcttgtcg atctgcctgg cgaacgcctg cccggcctgc
      721 atcacctccg gagatgtctg cgggccctcg acgatctcca ccagcatcat catcgcgacc
      781 gggttgaaga agtgcagccc cacgaggcgc tcgggacgct cgagcacgcc ggagagttcc
      841 tgaagcggga tgctcgacgt gttggaggcg aggatcgccc cctccttcat ccggggctcg
      901 atgccggcga agagctttct cttgacctcc gcattctcga cgatggcctc gatgacgagg
      961 tccgccttcg gcacgccatc gcccttcacg tcggggatga ggcggtcgaa cgcggcccgg
     1021 acgaggtgcg gctgcttcag gcgcttcttg aagagcgcat gcgcgcgctg gatggcgggc
     1081 gcgatgcgct cgggcccgag gtcctggagc gtgacggtga ttcccttgag cgcgcaccac
     1141 gcggcgatgt cgccgcccat ggcgcccgcg ccgaccacgt gcaggtggcg gatcggctcg
     1201 ccgccgcggt cccgtgcatc gattggcacg gacccgcgct tgccgaacgc cttcatccgg
     1261 tcctgcagct tgaagaggcg gatgaggttg cgcgtcgtgg aatgcgcgaa gaggctcgcc
     1321 atcgacgccg ggtgcccgac cgggatgttg cgcacgtccc cgccgaaatc cttccacagg
     1381 tcgatgatcg cgtagggcgc cgggtaatgg tcctggcgcg ccttctcggc gaccttcttc
     1441 cgggacatcg acgccacgaa cgagcgcacc accggccagt cggtgattgc cgccgagagc
     1501 ggcggcttgt gcggcggcgg cggactggcg aggaacatgc gcaccgcgtt gccgaagtgg
     1561 cgtttctgcg tcacggcatc caccagcccg agcttcttcg cccggcgccc gtcgacgccg
     1621 cggccggtca gcatgaggtc gagggcgttg gccgcgccga tgagcgccgg catgcgcttc
     1681 gctccgcccc agcctggcac gatgccgatg agcacctcgg gcagtgccat gcgcgtcttc
     1741 gggccgtcgt cggccatgcg atagcggcag gcgagcgcca gctccgtgcc gccgcccatg
     1801 cagaagccgt ggatcatcgc gagcgtcggg aagggcagtg ccgcgatcct gtcgaagacg
     1861 gcgtttccga gcgtggtgaa ggccatcgcc tgctccgcgc tcgagatcgt ggtgaactcg
     1921 tcgatgtccg ccccggcggc gaagccgttc tccttggccg agaggatggc gagccccttg
     1981 ggcggcatgg cggcgaggtg atcggcgatg cggtccagtt cctgcagcac ttccgtggaa
     2041 aaggtgttgg tcgcggtgcc ggcgcggtcg aaccacagcc aggcgaggct ctgcgcgtcc
     2101 atttcgagct tccagtgctt caggtccatg ttcttccttg gggggcctag ggccgttcga
     2161 gaagcatcgc gccgccctgc ccgccgccga tgcacagcga cgccacggcc cgccggccgc
     2221 cggtgcgctc gagtaccttg agcgcgtgca gcacgacccg cgcgcccgac gcaccgatgg
     2281 gatgtcccat cgcgatggca ccgccatcca cgttcagctt gccacggtcc agcctcccga
     2341 aggcccggtc gagcccgaag tgcgtcccgc agtactcgtc gctttcccac gcggcaaggc
     2401 agccgagcac ctgtgcggca aaggcctcgt tcacctccac gcagtcgatg tcgtcccagc
     2461 cgaggtggtt tctctgcagc atcggggtga tggcgtgaac cggacccagg cccatctccg
     2521 cgggatcgag cgcgccccag ttggcgtcca cgacgcgccc gagcgtcgtc aggccgtatt
     2581 cctgcacgac ctcctccgcc gcgagaatga gcatcgccgc gccgtcggtg atctgcgagc
     2641 tgttgcccgg cgtcacgttg ccgtaggttc gatcgaacac gggcttgagc ctggcgagct
     2701 tctccacggc ggaatcccgc cgcagcccgt cgtccgtcga gtaaaccgtc ccgtccggcc
     2761 cgtagatggg ttcgatctcc gcaaggtgcc cgttgtcctg cgcgaacgcg agccggcgat
     2821 gcgattccac cgcgaactcg tccatcatct ggcgggtgat gccgaagcgc gtggcgacga
     2881 tctcggcggt cgagcccatc gacagcttca ccacggggtc ggtgaggccg cgcaggatgg
     2941 cgatcacggg agcgaggttg gcgagccgga atttcgcggc cgtcgccgct ttctgcccga
     3001 aggacttcgc cgcgtaccag tcggccagcc agttcaccat cgccggcccg aagagcagcg
     3061 gcgcgtggct catggcgtcc gtgccgcccg cgagcaccag gctggaacgc cctgcctgga
     3121 tattgaggca cgcggtgtcg atcgcctgca tgcccgaggc gcagttgcgc atgacggtcc
     3181 aggccggcac cttttcaccg cagccgaggc gcagcgaaac cacgcggccg atgttggcct
     3241 cgtcggcgga gggcaacgca gcgcccacga tcacctcgtc gaacgccgtc gcgtcgaagg
     3301 gctggcgcgt gacgagcggg cgccccgcgg ccacggcaag gtcggcgccg gtgaacgggc
     3361 cgagcccctt cttcgccttg aggaacgggg tgcgggcccc gtcgactaca tggacgcggc
     3421 gcgcagccat ggggctttct cctggttcga cttacgtgac aggtcggtcg ggaaatcgtc
     3481 gaccatgatg accgagcgcc gcagccggtc ggtgcgcgag aggtggtcga actcggcgag
     3541 cgtgatgacc ccactcttca cggctgcgtc gcgccgctcg gcgagcgtgc gctgcgggag
     3601 cttgccttcc ttctgcgcgg cgcggatctt cgcctcgatg ggctcggcct tgatcacggc
     3661 ttccaaggcg aattcgaggc gcccgatggc gtcgtcctcc ttgcggggca gatagattcc
     3721 cgccgtgagc cggtcgcgca ccgcgcccgg ctcgatgagc aggcgcgcca cgcgggaggc
     3781 ccacttgtcg tgcggcgcgg tcagtgtgag gcccttcgga aaggcgaggt ggcgcagcac
     3841 ccacgcgatc ggccggttcg gaaaattcgc gagcacgccg tccatcgcga cctgcagctt
     3901 gaacgaactg tcgtacatcg cccaggtgag gagcggcaag tcctccttcg ggcaaccgtc
     3961 gtcgtggaag cgcttcacgg tggccgacat catgtagagg tgggcgagga tgtcgcccaa
     4021 gcgtgccgag agcatttcct tcctcttgag gctgccgccg atcacgaaca tcgacatgtc
     4081 cgcgagcagc gcgaaggcgg acgagaagcg cgtgaggatc tgcaggtagc ggcgcgtgtc
     4141 cgcggagccg ggaaccgata tgcccttgcc gccggtgagg ccgagccaca gggcgcgcgc
     4201 gccgttggat gccgtgaatg cgacgtgccc ccagagggcg cggtcgaagg ccacgaggtc
     4261 gtcggctccc gcggcgcgga tttccttcag cacccacggg tggcagcgga tggcgccctg
     4321 gccgaagatg atgagcgagc gcgtgaggat gttggcgccc tccacggtga tggcgatggg
     4381 caccgtctgg taggcgcggc cgaggtagtt gttggggccc atgcagatcc ccttgccgcc
     4441 gtgcacgtcc atcgcgtcgt tcaggatcga gcgcatgcgc tcggtcatgt ggtacttgca
     4501 gatggccgag atgacgctcg gcttctcgcc catgtcgagc gcggccatgg tcatgcggcg
     4561 catgccgtcc atgaggtagg tcgagccgcc gatgcgcgcg agcggctcct cgatgccctc
     4621 gaacctgccg atgggcgtct tgaactgcga acggatgcgc gcataggccc cggtgacgcg
     4681 cgtgccgatc ttgccggcgg ccatggacat cgacggaagc gagatggagc ggcccgcggc
     4741 caggcactcc atgagcatgc gccacccggt gcccgcctgg gccttgccgc cgatcaccca
     4801 gtccagcggg atgaagacgt ccttgcccgt ggtgggcccg ttcatgaacg cgcagcccaa
     4861 cgggtcgtgc cgccggccca cgacgacgcc ggcgtgcgaa gtcgggacga gcgccagcgt
     4921 gatgccgaca tcctcctgcg cgccgaggag cttttcggga tcgtagagct tgaaagcaag
     4981 gccgagaagg gtcgccaccg gcgccagcgt gatgtagcgc ttttcccagg taagccggat
     5041 gccgagcact tcccggccct cgtgcaggcc cttgcagacg atgccgaagt ccggaatgga
     5101 cgcggcgtcc gagccggcat cggggttggt cagcgcgaag caggggattt cctggcccct
     5161 ggcaaggcgc ggcaggtagt ggttcttctg ctcgtccgtg ccgtagtgga tgaggagttc
     5221 ggccggcccc agcgagttgg gcaccatgac cgtcacggcc gcagcaccgc tgcgggtcga
     5281 tatcttggtg acgatctcgg aatgcgcgaa ggcggagaag cccaggccgc cataggattt
     5341 cgggatgatg atgccgagga agcccttgtc gcggatgaat ttccacgcct cgggcgagag
     5401 gtccaggcgc tcgtggttga tgacccactc gtcgagcatt ccgcagagct cctcgacggg
     5461 gccgtccacg aacgcctgtt cttccgcggt cagcttcggc gccgggtagg cgagcagctt
     5521 ggtccagccg gggctgccgc tgaaaaggtc ggcgtcccac cacacgctcc ccgcgtcgag
     5581 cgcttcctgc tcggtctgcg aaacctgggg gagaacccgc ttgaagacgc cgaagagcgg
     5641 ccccgtgacg atcgcgcggc ggatgggctt caccacggcg agcgcggcga tgagcgccac
     5701 cacgacccac gccgcggcaa gaacaccggc aggcgcagcg gtaaacccgg cgaggcaagc
     5761 cgcaccgacg gccagcgccg cggaccacgc gacggcgttt gcgcggtgat aggcgagcgc
     5821 ccacgcgacg gcgatcgcgg cgaggaccga caggatgatg ggcagcatcg tcattctcct
     5881 tccccaatga agcgcccgcg aagcgggcag gcattgctcc tcgacgcccc ggccggggct
     5941 acttccagtc gcccgaggcc agctcgagct tcttcgcatc gccgagcggc gccttgaggc
     6001 cggccgcgag gaacggcgcc aggcgctgca gcagcatttc gtcgttgtcc ttgtcgtgcg
     6061 ggatcacctg cgccatgagc ttgagcgcat ccgtgcccgc gagcgtgtag gaaagtgcgc
     6121 ccatcacgaa atgcaggcgc caggtgagtt cctgcttcgt gaggtggggc agtgccttct
     6181 ggaacgcgtc cttgaagcgc gcgatcatct cggcgtactg ggccgacagg aaggtgcgga
     6241 tgaagggcgc cgggtcggca taggcgcgcc ccaggacgcg aaggaagtcc ttgccgccgc
     6301 gcctctcgtc gcgcgagagc ctgagtgccg ggatgagcat cgccgagagg atcttctcgc
     6361 aggtgagcgg ccggccgccg gcctcgcgct cgtagcgcgc gagcaggtcg attcgctcct
     6421 ggttcatcgg atcgagccgg cgcgtgagca ccgcctggaa aagctcttcc ttcgtgccga
     6481 agtggtagtt gaccgccgca agattcacac cggccgcgga tgtgagctgc ctcaggctcg
     6541 tcgcctcgaa gccgtgctcc gtgaacaggg attccgccgc gtccaggatg cgcgacttgg
     6601 tgtcctgcga cagggcatcc atcgcaagcg ttcggcgggc ggggtgctgc gcgggcatgg
     6661 cgtctgtctt ccggatatgt ggcaagggcg tcggaatcgc gtcaaacgct cgattcaaac
     6721 gcccgtttga aactatccta gggcgcgcgg ccggccattg tcaaatccct gcgcgtaccg
     6781 ccttcggcta cacttccgcc gcaccatgca tcgagttctc ctgctttcgg tcgcgctcgc
     6841 cgcggccgcg atcccccgac ccgccgacgc gatccttgtc cgcgccgacc gggacgatgc
     6901 ggagtaccgc gagctggcca cgcgctatcc atccgccgtc ggcctgggcg ccgcggcagg
     6961 cacgggcgtc ctcatcgcgc cacgctggat gctgaccacg gcccaccgcg cggcggcgtt
     7021 gcgcgcgatg aagggaccgc tgctcatcgc cggccgtccc catgagatcc aggacgtcct
     7081 tgtccatccc gactggaagc cgggtggcga atcggacatc gcgctcatct tcctgcgcac
     7141 cgccgccgac ggtgtggagc cgacaccgat ctaccgcttg cccgacgagg agggccaggc
     7201 cgcgcgcatc gtgggcgtgg gcgagacggg ccggatcggc gagaaggcca cgaagccgga
     7261 cggcctggcg cgcgcggcca tcaacaccgt cgatcgcgtg acgccccgca cgctcgggat
     7321 gcgcctcaag ggtcccgagg acgcatccga cctgcagggc gccctggccc cgggggacgg
     7381 cggcgccccg gccttcttcg aagccggcgg ccgcattttc gtggcaggca tcgcctccac
     7441 acccgaaggc gccaatgccg gcggccatgc acgcaacgtc ggcgactggg aggtctacgc
     7501 gcgcgtctcc gccttcgcgg cctggatcga cgacacgatg tcgaaggtgg ctgtcgagga
     7561 agccgcgcgc gagacggcga aaccgcggcg ctgaacgatc aggcgcgcga aagcgcctgg
     7621 tccaggtccc agagaatgtc gtcgagggtc tcgaggccga tggagagccg caccatgtcg
     7681 ggcggcacgc cggccttcac caggtccgcc tccgggatct ggcgatgcgt ggtggacgcg
     7741 gggtggatca cgagcgattt cgcgtcgccg atgttggcca ggtggctcac gaactgcagc
     7801 ccctcgatga acttcacgcc cgcctcgtaa ccgccgcgga tgccgaacga gagcagcgcg
     7861 cccgcgccct tcggcatgta gcgtttcgtg agggcgtggt acttgttgcc ggcaaggccc
     7921 ggatagttca cccacgcgac cttgcgatgg tcgcgcagga agttcgcgac acccatcgtg
     7981 ttggcgcagt gccggtccat gcgcacggcc aacgtctcca gcccctgcaa caggagccag
     8041 gcattgaagg gcgagatggc ggggccgaac acgcgcagga tctccatgcg cgccttcatc
     8101 gtgaagccga agtcgccgaa ggtctcgtaa aagcgcacgc cgtggtaacc ggccgagggt
     8161 tccgtcatcg acgggaactt gccgttgtcc cacgggaact tcccgctctc caccaccacg
     8221 ccccccatgg tcgtgccgtg gccgccgatg aacttggtgg ccgaatgcac cacgatatcc
     8281 gcgccatgcc tgaagggctg gcacaggtag ggggacgccg ccgtgttgtc caccatgaac
     8341 ggcaccccgg cctcgcgcgc gatggcgccg accgcggcga gatcgaccat gttgatggcg
     8401 gggttgccca gcgtttcgga aaagatcacc ttcgtgttcg ggcgaagggc ccggcggaag
     8461 ttttccgggt cgtcgggatc gacgagcgtg gcctcgatgc cgagcttcgg aaagttgacg
     8521 gcgaacatcg tgtgcgtgcc gccgtagagc ttcgacgacg atacgatgtg gtcgccggcc
     8581 tggcagatgt tgaggagggc caccatttcg gcggcctgcc cggaggcggt ggcgacagcg
     8641 gcgcgcccgc cctcgagagc cgccatgcgc tcctcgaaca tcgcggtcgt gggattcgac
     8701 aggcgcgtgt agacgttgcc gaaggtctgc aggttgaaca ggctggccgc atggtcggcg
     8761 ctgtcgaaga cgaacgacgt cgtctggtag atcggcgccg cgcgcgcgcc cgtctgcgca
     8821 tccgggatct ggcccgcgtg cagcgccagc gtctcgaagc cgaactcgcg atcggccatc
     8881 gcttacttcg gcacttccga agaggccctc gtcctgtcga gcaccgtcat cgcggtggac
     8941 acgaacgtgg aaacgtcggt gaggttcgcc ggcacgatga gcgtgttgcc ggtcttcgcg
     9001 agattcgcga aggcctcgac gtatttttcc gcgaccttga ggttcgcggc cagcatcccg
     9061 ccctcggcgc cgatggcggc cgcgacctgc cgcaccgcgt tggcggtggc ctcggccacg
     9121 gtcgtgagcg ccagggcctc gccctgcgcc ttgttgatcg cggccgtctt gtcgccctcg
     9181 gactgcagca ccttggcctg cttggcgccg tcggcgatgt tgatctcctg ctggcgctgg
     9241 ccctcgctct tggcgatgag ggcgcgcttc tcccgctcgg cggtgatctg cgcctgcatc
     9301 gagcgcagga tggcctccgg cggcgtgagg ctcttgattt cgtagcgcag caccttgatc
     9361 ccccaggtgc gacccgcgtc gtcgaggacg gagacgacca tgtgattgat ctcgctgcgg
     9421 ctctcgaagg tcttgtccag ctccatcttg cccacctcgc tgcgcagcgc ggtctgcgcg
     9481 agctgcgaga tggcgagcac gtagtccgac gagccgtagg aagcgagctt cgggtctgac
     9541 acctggtaat agatgatccc gtccaccgcg agctgcgtgt tgtccttggt gatgcagacc
     9601 tgctcgggga tgtcgagcgg cacctccttg agcgagtggc ggtaggccac gcggtcgatg
     9661 aacggcacga tcacgttgag gcccgggggc aggacctcgt ggaacttgcc caggcgctcg
     9721 atcacccagg cctgttgctg gggaacgaca cggattccct cgatgatcag caccacgatc
     9781 atcgcgatga ggaacgtggc gacggtgatg ccgacgcttt gcgtgaggaa tgcggcggcg
     9841 accgcgacga tgacagcgac gagtaccttc cagaacatgg tgcctccctg gcttcttgtt
     9901 ggtctatttc gacgtcggcg cggcgcccac gaggagcgtg ctgccgtcct gcccgttgat
     9961 gacgagtgtt gcgcccggct cggggtgcgc tcccgcagcg acccgtgcgt cccacgtcgt
    10021 gccccggtac ttcacccggg ccgttcccga gctcgcgtcc acccacgatt cgaggaccac
    10081 gggctggccg aggtcgagga agttgtcctt cgcactgccc ggctggcgcg tgcgatgcca
    10141 gcgattcacg aaccacgtcc cgaccagcgc caccgcgcag gcgccgatcg cctgcccgag
    10201 gtagccgccg cccgcccacg cgaccgcggc gccggcgaag gcgcccacgc cgatcaccag
    10261 caggtagaag gtcccggaca ggagctcgat gatcaccagc acgaaaccgg cgatgatcca
    10321 ggcgaggtag ggttccatga cgacctttct tgtctagtat tgacttgtca ccggcaagcg
    10381 acgaacggga cttgttctct ccggcgatcg caaggcgctt gcggcacgga ggcggcgcat
    10441 caggcgggtc gcttggcgga tatcaccttc gtgttcaccc ccgcccaacc cagcccgccg
    10501 aaaaggcgcc cgtgctcgat cttgaggcag cggtcctcga cgacctcgag accggcggcc
    10561 tcggccttgc gccgggcctc ctcgctcctc acgccgagtt gcatccagag caccttcgcg
    10621 ccgatcgcga tggcctcgtc ggcaagggcg gcgatctcct cgctcctgcg gaagcagtcg
    10681 acgagatcga ccttctccgg gatgtcggcc agcgatttgt agcacttctc gcccaacacg
    10741 ctgtcgtaca tcggattcac cgggaagatg cggtagccgt ggtcctgcat gtacttggcg
    10801 gcgaagtagc tcggacggta ccagttggcc gagagcccga ccaccgcgat ggtgcggttg
    10861 tcacgcagga tgcggcgcag cttcgcgatg tcgtccatgg ttccgtggga aactgaaagc
    10921 ctctcgagga ttctacccgt catgcgccaa tcgctcatcg ccttcgccgt cgccgtcgcc
    10981 gtctgcatgc ccgcgcgggc gagcaatcac gacaccgtat cgccgcccat cggcgcgggc
    11041 ccgttcaagg tggcgtgctc gaacatcgca caggacgagg cgctgatcgc ggcgctcggc
    11101 tcgacgccgg ccgagatctg ggaaggccgg ccgcgcgacg ggcaggcgcg atacatttcg
    11161 caggttctcg ccgcgcccgg cacggcgctg caattcgagg cgccggtgcc ggacatccgc
    11221 gacatctacc cgaggctcgc gggggaaaac gtccactacg tggcgatcgt ctgccaccct
    11281 acgccgcagg cgaatgcgga tgccgactac gtccttccgg gaacgagcga catcgtgccg
    11341 cacatgcaac tgcctggcgc gtcgccgcgg ctgatcggtc tcgcggaata cgcggcaacg
    11401 tacgggatcg ccgtggaccc cgcaatgccc tcctccgcac cggcgcgctt gcccctcatc
    11461 gtcttctcgc acggcctggg ggggagcccg gtcagcccgg gctatgtcga tgcgatggtc
    11521 gacctcgcct cgcacggctt catggtggcg gcggttttcc acggcgattc gcgcttctcg
    11581 cgcatccgcg tcgaggacct cggccagttc gtgaccctgc tcctgaactt cgacggtttc
    11641 gtcgaaatgg agttgctgcg tccggtgtcg ctgaaatcgc tgctcgacac cctcctgcag
    11701 cacgcgggat tcgcacccgg catcgatgcg gagcgcatcg gcggcttcgg cgccagcctc
    11761 ggcgggcaag ccgtggcgaa cctgctcggc gcgcggctca cgaccagcct gcgcctcgcc
    11821 tgccgggaca ccgtcaccga cgcgcgcgtc aaggcggcag tgggactcgt gccttacgcg
    11881 gggcagacct tcctgcccgc cttctgcgac gaccagggcg gcgcggtcgc agtgaagcgc
    11941 ccgtatctcg ccctctccgg aacggcggac acgacagcgc ccatcgcgat ggtggagcag
    12001 gcagtcaatc gcttccaggg ctcgcgttac ctggtcgagc tgcagggcgt ccagcacgag
    12061 tacctgcccg aatatcgcgg cgacgtgatg acctggaccg tgacgttcct gaacgcctac
    12121 ctcggagtcg ccgcggatcc cggggcgatc gcccgcctca tccggctgaa gagcgtcacc
    12181 ggcggtccgc aggacgcgct ccgcgtggac gcgcacattc cgttcccgcc gggtccggag
    12241 gaggcgctgg tcctggagtt ctacaacgat ccgctcgccc actacttcat cgccgcgggc
    12301 gccgacgaga tcgcctcgat cctcggcgga ggcgcgggtc ccgggtggca actgaccggc
    12361 cacgcgttca aggcgtatcc gcggcttccg gcggacgcct tcaccacggc cgtcccggtg
    12421 tgccggttct atggcgcctt ggctggcggc ccgaattcgc acttcttcac cgcggacgct
    12481 cccgagtgcg acctcgtgaa gcgatccggg ggctggtact acgaggggac gggcttctac
    12541 atcacgccgg tcggcgcgga cgggcgctgc ccatcgggct acctccaggt gctgcgcgcc
    12601 tacaaccagg gtttcccgcg caacgactcc aaccatcgct tcacgaccag cgattccacg
    12661 tggcgggaaa tggaacggca cgggtgggtc ctcgaaggcg ccgtcatgtg cgcccgggcc
    12721 tgaccacgcc gggggccacg tcgccggcac cggtggcctt ttcgcgcgcg gctgcatagt
    12781 tcgcccacca gtcccgcgct tcctcctcgc gccacggccg ctcgcgcgcc acctcggcca
    12841 gcaccgcggc cacttccgcg atcgaccgcg gccgttcctc gcgatccttg gcgaggcatc
    12901 gtgccaccag ggcctcgagg atcgcgggga cgtcctgcgc gccgcgatcc gcgatcgagg
    12961 gcggggcgac attgagcacc tggtagacga ggtcgtggtc gctctgcgtc tcgaagacgc
    13021 gccgcccggt gaggaggaag aacgccaccg cccccagcgc gtagatgtcg gcccgggcat
    13081 cggcgtcggt cgggttgcgc aggcgttccg gcgccatgta gagcggcgtg cccaggacgc
    13141 gcgcgaactg cgtgatgtcc cgcgtgtgca cgttctcgag ttccttcacg aggccgaagt
    13201 cgagcacctt cacgacgtcg aattcgccgc catggtggca gagcatcagg ttgtggggct
    13261 tcacgtcccg gtgcaccagc ccgcgctcgt gcgcttcctt cagcgagttg cagacctgcc
    13321 gcagggcgtg caccgcgcgc ggtaccggca ggcggccctc cttctgcacc agctcctcga
    13381 gcgacaggcc gtcgaggtat tccatcgcgt agtagaaagt accgtcgcgc gtgcgcc
//
LOCUS       DASUEV010000120         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_250842, whole genome shotgun sequence.
ACCESSION   DASUEV010000120 DASUEV010000000
VERSION     DASUEV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250842"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..556
                     /gene="aceE"
                     /locus_tag="VFV55_03975"
                     /pseudo
     CDS             23..556
                     /gene="aceE"
                     /locus_tag="VFV55_03975"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
     gene            933..2579
                     /gene="aceF"
                     /locus_tag="VFV55_03980"
     CDS             933..2579
                     /gene="aceF"
                     /locus_tag="VFV55_03980"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX4943486.1"
                     /translation="MTCAPSPTRSASSSRGATRRSAPTGSAAATRARTCAASSRWTAT
                     TSSSRPSRRSPRKARSRPRRWRRPSRNTASTPRSPRPGPSDPGQFENKQQHEERALAV
                     IEVKVPDIGDFKDVPVIELLVKAGDTVQKEASLVTLESDKATMDVPAPTGGVVKELKV
                     KLGDKLSEGSIVLLLESAGALAAVPAAAPAAPAPAPSPATPAAVPAPAAPAAPAPAGT
                     IPAAPPSAPAHRPAPVPPLPAAEAGAPSHASPGVRRFARELGVDLALLKGSGPKGRIL
                     KEDIQEFVKSALSGAGATAPRASGGGLADLGLPAWPKVDFAKFGPVEAQPLSRIKKIS
                     GPALARNWVMIPHVTQFDEADITDLEAFRARVNEENAKSGTKVTPLAFLIKACVAALK
                     KFPDFNASIDGDNLVLKRYWHIGFAADTPNGLMVPVIKDADKKGVVEIAKETGELAAK
                     AREGKLGPADMQGGTFSISSLGGIGGTAFTPIINAPEVAILGVSKAAMKPVWNGKEFA
                     PRLMMPLSLSYDHRVIDGAAAARFTTYLAQVIADLRRGML"
     gene            2589..4388
                     /gene="lpdA"
                     /locus_tag="VFV55_03985"
     CDS             2589..4388
                     /gene="lpdA"
                     /locus_tag="VFV55_03985"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX4943487.1"
                     /translation="MAILEIKVPDIGDFKDVPVIEILVKPGDAVKKEASLVTLESDKA
                     TMEVPSTHAGTVKELKVKVGDRLSEGSVILLLESAETVQAARATAPAAATSAPSPTAT
                     KPATGNQTTLREALTTTLASLDADLHAEVLVLGAGPGGYTAAFRAADLGKKVVLVERY
                     ATLGGVCLNVGCIPSKALLHAAKVIADAEDMAQHGVSFGAPKVDLDGVRKFKDGVVSK
                     LTRGLASLAKARKVTVVEGRAQFASPHTLEVETKEGRKVVSFDHCIIAAGSQSARIPG
                     LPYDDPRLMDSTSALELKDVPKRLLIIGGGIIGLEMATVYDALGSKVTVVEFMDRLIP
                     GADPDIVKPLAKRIEKRYEKILLKTKVAKIDALKEGLCATFESADGAAPAPAPEVFDR
                     ILLSVGRRPNGKNIAAEKAGVTVNDRGWIPVDNQMRTNVSHIYAIGDIVGEPMLAHKA
                     THEGKLAAEVIAGMAHHVWEARTIPSVAYTDPEVAWMGLSEPEAKARGIEYEKAVFPW
                     AASGRAIGMGREEGLTKILFEKGSKRILGAGIVGINAGELIAETVLALEMGADAEDIG
                     LTIHPHPTLSETVFFSAEIEEGSITDMMPKARS"
BASE COUNT          813 a         1564 c         1548 g          649 t
ORIGIN      
        1 gaccacgatg gccttcgtgc gcatgctcac ctcgctcgtg agggacaaga acatcggccg
       61 caacatcgtg cccatcgtgc cggacgaatc gcgcacgttc ggcatggagg gcctgttccg
      121 ccagctgggc atctactcca ccgtgggcca gctctacgag cccgaggaca gcgaccagct
      181 catgttctac aaggaggaca agaacggcca gatcctccag gaaggcatca acgaggcggg
      241 cgggatgtgc tcgtggatct cggcggcgac gtcgtactcg acgaacaacg tgccgatgat
      301 tcccttcttc atcttctact cgatgttcgg cctgcagcgc gtgggcgacc tcgtgtgggc
      361 ggcgggcgac atgcgcgccc gcgggttcct catgggcggc cccgcggggc gcacgacgct
      421 caacggcgag ggcctgcagc acgaggacgg gcacagccac atcctctcct cgatgatccc
      481 caactgccgc tcgtgggacc cgaccttcgc ctacgaggtc gtggtgatca tccaggacgg
      541 cctgcggcgc atggtggagg accaggagga cgtgttccac tacatcaccg tgatgaacga
      601 gaactacccc cagccggcgc tgcccgaggg cgcgcgcgag ggaatcctca agggcatgta
      661 caggctgcct ggcggcggcg ccggcaaggg gccgcgcgtg cagcttctcg gcagcggcac
      721 catcctgcgc gaggtgatcg cggcggccga cctgctggcc gccgactggg gagtcgcctc
      781 cgacctctgg agctgcccca gcttcaccga gcttcgccgc gacggcatcg ccacggcccg
      841 ctggaacctg ctgcacccga ccgggaagcc gcgcgcgtcg cacgtcgagg agtgcctggc
      901 gggcacgaag ggaccggtgg tcgcctcgag cgattacgtg cgcgccttcg ccgaccagat
      961 ccgcgagttc gtcccgcggc gctacaaggc gctcggcacc gacgggttcg gccgcagcga
     1021 ctcgcgcgag aacctgcgcc gcttcttcga ggtggaccgc cactacgtcg tcgtcgcggc
     1081 cctcaaggcg ctcgccgagg aaggcgagat cccggccgcg aaggtggcgg aggccatcgc
     1141 gaaatacggc atcgacgccg agaagcccgc gccctggacc gtctgacccc gggcaattcg
     1201 aaaacaagca acaacacgag gagagagcct tggccgtcat cgaggtcaag gtccccgaca
     1261 tcggggactt caaggacgtg cccgtcatcg agttgctggt gaaggccggc gacacggtgc
     1321 agaaggaggc ctcgctcgtc acgctcgaga gcgacaaggc caccatggat gtacccgcgc
     1381 ccaccggagg cgtggtgaag gaactcaagg tgaagctggg cgacaagctg agcgagggca
     1441 gcatcgtcct tctcctcgag tccgcgggcg cccttgccgc agtacccgcc gcggccccgg
     1501 cggcaccggc ccccgcgcca tctcccgcga caccagcggc tgtcccggcg ccagcagcac
     1561 cggccgcgcc agcgcccgca gggaccattc ccgcggctcc gccgtctgca ccggctcaca
     1621 ggcccgcacc tgttccccct ctgcccgcgg cggaagcggg cgcgccttcg cacgcgagcc
     1681 cgggcgtgcg ccgcttcgcg cgcgaactgg gcgtcgatct cgccttgctg aagggatcgg
     1741 ggcccaaggg acgcatcctc aaggaagaca tccaggaatt cgtgaaatcg gctctctcgg
     1801 gcgccggtgc gacggccccg cgcgcttccg gcggcggcct ggcggacctg ggcctgcccg
     1861 cgtggcccaa ggtcgacttc gcgaagttcg gcccggtcga ggcgcagccg ctttcgcgca
     1921 tcaagaagat ttccggcccg gcgctggcgc gcaactgggt gatgatcccg cacgtcacgc
     1981 agttcgacga ggcggacatc accgacctcg aggcgtttcg cgcgcgcgtg aacgaggaga
     2041 atgcgaagtc cggcaccaaa gtgacgccgc tcgccttcct catcaaggcc tgcgtggcgg
     2101 cactgaagaa gttcccggac ttcaacgcct ccatcgacgg cgacaacctc gtgctcaagc
     2161 gctactggca catcggcttc gccgccgata cgccaaacgg actgatggtc ccggtgatca
     2221 aggacgccga caagaagggc gtggtcgaga tcgcgaagga gacgggcgag ctcgcggcca
     2281 aggcgcgcga gggcaagctc gggcccgcgg acatgcaggg cggcaccttc tcgatttcct
     2341 cgctgggcgg catcggcggc accgcgttca cgccgatcat caacgcgccg gaagtggcga
     2401 tcctgggggt ttccaaggcg gcgatgaagc cggtgtggaa cggcaaggag ttcgcgccgc
     2461 gactcatgat gccgctctcg ctatcgtacg accaccgcgt catcgacggt gccgcggcgg
     2521 cgcgcttcac cacctacctc gcccaggtca tcgccgacct gcggcgcggc atgctctagg
     2581 agcgcgccat ggccatcctc gagatcaagg tccccgacat cggcgacttc aaggacgtcc
     2641 cggtcatcga aatcctggtg aagccgggtg atgccgtgaa gaaggaggcg tctctcgtca
     2701 ccctcgaatc cgacaaggcg acgatggagg tgccctcgac gcacgcgggc accgtgaagg
     2761 aactgaaggt caaggtgggc gatcgcctct ccgagggctc ggtgatcctg ctgctcgaga
     2821 gcgccgagac ggtacaggcg gcccgtgcga ccgcgcccgc tgccgcgact tccgcgccat
     2881 cgcccactgc cacgaagccg gccaccggca accagaccac gctgcgcgag gcccttacca
     2941 ccacgctcgc gagcctggac gcggacctgc acgccgaagt gctcgtgctc ggcgccggcc
     3001 ccggcggcta cacggcggca ttccgcgcgg ccgacctcgg caagaaggtg gtgctggtcg
     3061 agcgttatgc gactttgggc ggcgtgtgcc tcaacgtggg ctgcatcccc tcgaaggcgc
     3121 tgctgcacgc ggccaaggtc atcgccgacg ccgaggacat ggcgcagcac ggcgtctcct
     3181 tcggcgcgcc gaaggtcgat ctcgacggcg tgcgcaagtt caaggacggc gtcgtctcga
     3241 agctcacccg gggcctcgcg agcctcgcga aggcgcgcaa ggtgaccgtg gtggaagggc
     3301 gcgcgcaatt cgcctcgccg cacacactgg aagtggagac gaaggaaggc cgcaaggtcg
     3361 tctcgttcga ccattgcatc atcgccgcgg gctcgcagtc ggcgcgcatt ccgggcttgc
     3421 cctacgacga cccgcgcctc atggattcca cgagcgcgct ggaactgaag gacgtcccga
     3481 agcgcctgct catcatcggc ggcggcatca tcggcctcga gatggccacg gtctacgacg
     3541 cgctcggctc gaaggtgacg gtggtggagt tcatggaccg cctcataccc ggggcggacc
     3601 ccgacatcgt gaagccgctg gcgaagcgca tcgagaagcg ctacgagaag atcctgctga
     3661 agaccaaggt ggcgaagatc gacgcgctga aggaaggcct gtgcgccacc ttcgaaagcg
     3721 ccgacggcgc cgcgcccgca ccggccccgg aggtcttcga ccgcatcctg ctctccgtcg
     3781 gccgccggcc caacggcaag aacatcgccg cggaaaaggc cggcgtgacg gtgaacgatc
     3841 gcggctggat acccgtcgac aaccagatgc gcaccaacgt ttcgcacatc tacgccatcg
     3901 gcgacatcgt gggcgagccg atgctcgcgc acaaggcgac ccacgagggc aagctcgcgg
     3961 cggaagtgat cgccggcatg gcgcaccacg tgtgggaggc gcgcacgatc ccgagcgtgg
     4021 cctacacgga tcccgaggtg gcctggatgg gtctgtcgga accggaggcg aaggccaggg
     4081 gcatcgagta cgagaaggcc gtgttcccgt gggcggccag cggccgcgcc atcgggatgg
     4141 gacgcgagga gggcctcacc aagatcctct tcgagaaggg ctcgaagcgc atcctcggcg
     4201 ccggaatcgt cggcatcaac gcgggcgagc tcatcgcgga gacggtgctt gccctcgaga
     4261 tgggcgcgga cgccgaggac atcggcctca ccatccatcc gcacccgacg ctttccgaga
     4321 ccgtgttctt ctccgccgag atcgaggaag gctcgatcac cgacatgatg ccgaaggcgc
     4381 gatcgtaggt acgaacaccg cacgcgagtc gaggaggcgg tggcggtgga agtcgatcgc
     4441 aatcctgggc acgcaatcgt gaccgtcgga tgggtgcccg gaaagtcgag gccaggcaga
     4501 acaagccagg cctcgacttt ccacccatct acagacgagg gcgctaacgc tttctcgtcc
     4561 gcgacggtcg cgtg
//
LOCUS       DASUEV010000121         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1066559, whole genome shotgun sequence.
ACCESSION   DASUEV010000121 DASUEV010000000
VERSION     DASUEV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066559"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFV55_03990"
     CDS             <1..445
                     /locus_tag="VFV55_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX4943488.1"
                     /translation="FQVDADGLLSVSAREKTTGIGASVVVKPSYGLTDDEVATMLREG
                     FDHAQADRDARALAEQRVEAEGLITAIRSAIAHDGDLLSSVERASLEAGLASLDVARQ
                     GTDHRALKSAIEALNRASESFAGRRMDRSVSRALAGRRVDEVSAK"
     gene            442..783
                     /gene="fdx"
                     /locus_tag="VFV55_03995"
     CDS             442..783
                     /gene="fdx"
                     /locus_tag="VFV55_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEX4943489.1"
                     /translation="MTRVTVLPHEALCPEGATFDAEPGTTLCDGLLTHGIEIEHACEK
                     SCACTTCHIIVREGFDSLEEASDKEDDLLDKAWGLEFTSRLSCQARVGGQPLTVEIPK
                     YTINQVSESKR"
     gene            780..974
                     /gene="iscX"
                     /locus_tag="VFV55_04000"
     CDS             780..974
                     /gene="iscX"
                     /locus_tag="VFV55_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEX4943490.1"
                     /translation="MKWTDTRDIAIALAEKYPDTDPKTIRFTDLHQWVMGLDGFADVP
                     GHSGEKILEAIQMAWIEEAE"
     gene            complement(1043..2428)
                     /locus_tag="VFV55_04005"
     CDS             complement(1043..2428)
                     /locus_tag="VFV55_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4943491.1"
                     /translation="MKLHILATLLAVALAPAAWAQSPAAAEKPTISKPCLNCHKASPG
                     ELQGAFENVAFKSQAIQLKIDAATEIVKFDPATIKVTDAGIAKPAEALYDIRKGHEAR
                     IAFVEKDGVKTATAIWFKGPIKIAPEKLAKFDEVAALVAKGPEAGNFVLVDSRPLPRV
                     QEGTIPGSVNLPFTTKGFDALAAEKLPADKSKAIIFFCQGITCMLSPNSLRRAEALGY
                     TNVKVYREGWPEWTQKKVGVLAGAHLKEAWIDKDMPHVLIDARPAAEAKAGFIAGAVT
                     ITPAQVKSALAKFPDKALKAPIMVYDGGAGKAAMEVASAITKAGYPNVNVVTGGFDGW
                     KAAGYPVAAGTPAAKVAYAPKPRPGDIGIEAFRKLVKGTPPDTLILDVRNQDEANAGM
                     IKGAKLVPDEEILARLAEIPKDKRIVAHCSTGVRAEMTYHKLKEKGYNVAYVKADIEI
                     DKSGKPTITAN"
BASE COUNT          438 a          866 c          898 g          482 t
ORIGIN      
        1 gttccaggtg gatgccgacg gcctgctctc ggtttcggcg cgcgagaaga cgacgggcat
       61 cggggcatcc gtggtggtga agccctccta cggcctcacc gacgacgagg tcgccacgat
      121 gcttcgcgaa ggattcgatc atgcgcaagc cgaccgcgac gcgcgcgcgc tcgccgagca
      181 gcgcgtggag gccgaagggc tcatcaccgc gatcaggtcg gccatcgcgc acgacggcga
      241 cctgctttcg tccgtggaac gcgcgtcgct ggaagccggc ctcgcctcgc tggatgtcgc
      301 gaggcagggc acggaccacc gcgcgctcaa gtccgcgatc gaggcgctca atcgcgcgag
      361 cgagtcgttc gccggccggc gcatggatcg ctcggtttcg cgcgccctgg ccgggcggcg
      421 ggtcgacgaa gtgtccgcca aatgacccgt gtcaccgtcc tgccgcacga ggcactctgc
      481 ccggagggcg cgactttcga cgccgagccg ggcacgacgc tgtgcgatgg cctgctcacc
      541 cacgggatcg agatcgagca tgcgtgcgag aaatcctgcg cctgcaccac gtgccacatc
      601 atcgtgcgcg agggcttcga ttcgctcgag gaggcgtccg acaaggaaga cgacctcctc
      661 gacaaggcat ggggcctgga attcacgtcg cgcctctcgt gccaggcgag ggtgggcggc
      721 cagcccctga cggtggagat cccgaaatac accatcaacc aggtctcgga gagcaaacga
      781 tgaaatggac cgacacccgc gacatcgcga tcgcgctcgc cgaaaagtac cccgacaccg
      841 acccgaagac gatccgcttc accgacctgc accagtgggt gatggggctg gacgggttcg
      901 ccgacgtgcc cggccactcc ggcgagaaga tcctggaagc gatccagatg gcgtggatcg
      961 aggaggcgga gtaggttccc gcgctgttcg ccaatgggaa aggggcgggt aaccccgccc
     1021 cctcttccgg tcgatgccgc gttcagttcg cggtgatcgt gggcttgccg gacttgtcga
     1081 tctcgatgtc ggccttcaca taggcgacgt tgtagccctt ctccttgagc ttgtggtagg
     1141 tcatctcggc gcgcacgccc gtcgagcagt gcgcgacgat gcgcttgtcc ttcgggatct
     1201 cggcgaggcg cgcgaggatt tcctcgtccg ggacgagctt cgcacccttg atcatgccgg
     1261 cgtttgcctc gtcctggttg cggacgtcga gaataagcgt gtcgggcggc gtgcccttga
     1321 cgagtttcct gaacgcctcg atgccgatgt cgccggggcg gggcttgggc gcgtatgcca
     1381 ccttggcggc gggggtgccg gcggcaaccg ggtagcctgc ggccttccag ccatcgaagc
     1441 ctcccgtgac cacgttcacg ttcggatagc cggccttggt gatcgccgaa gccacctcca
     1501 tcgccgcctt gccggcgccg ccgtcgtaga ccatgatcgg ggccttgagc gccttgtccg
     1561 ggaatttcgc gagcgcggac tttacctgcg caggcgtgat cgtgaccgca cccgcgatga
     1621 atccggcctt tgcttcggcc gccgggcgcg cgtcgatcag cacgtgcggc atgtccttgt
     1681 cgatccacgc ctccttcagg tgggcgccgg cgaggacgcc gactttcttc tgcgtccact
     1741 cgggccagcc ttcgcgatag accttcacgt tggtgtagcc cagcgcttcg gcgcgacgga
     1801 gcgaattggg gctcaacatg caggtgatgc cctggcagaa gaaaatgatc gccttggact
     1861 tgtctgccgg cagcttctcc gcggccagcg cgtcgaagcc cttggtggtg aacggcaggt
     1921 tcaccgagcc gggaatggtg ccttcctgca cgcgcggcag cgggcgcgag tcgacgagaa
     1981 cgaaattgcc ggcctccggg cccttcgcca ccagtgcggc gacctcatcg aacttcgcga
     2041 gcttttcggg ggcgatcttg atcggcccct tgaaccagat cgccgtggcc gtcttcacgc
     2101 cgtccttctc cacgaacgcg atgcgcgcct cgtgtccctt gcgaatgtcg taaagcgctt
     2161 cggccggttt cgcgatgccc gcgtccgtaa ccttgatcgt cgccgggtcg aacttgacga
     2221 tctccgtggc ggcgtcgatc ttcaactgta tcgcctgcga cttgaaagcg acgttctcga
     2281 acgccccctg caattcgccg ggcgaagcct tgtggcagtt caggcacggc ttcgagatgg
     2341 tcggtttttc cgcggcggcg ggtgactggg cccacgccgc gggtgcgagg gcaaccgcga
     2401 gcagggttgc gaggatgtgc agcttcatga cttctccttc gatgacgaaa tggtgtgtgc
     2461 acaggcacga ccacgacgtg gttgggaaca ctcgtcgtgc ggggcgtgac cgatgttcac
     2521 catttgacgc ccgacagcgg gcggtgccgt gacctacatc aacaggcgct tatatgccct
     2581 gcgggggacc ggctcgatgc cggggcgaag ggcaaaaaaa aaaggggcag gaaacctgcc
     2641 ccacaaggcg cactcttgaa actggaggag agactggccg gcct
//
LOCUS       DASUEV010000122         5467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1525112, whole genome shotgun sequence.
ACCESSION   DASUEV010000122 DASUEV010000000
VERSION     DASUEV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525112"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="VFV55_04010"
     CDS             complement(<1..1852)
                     /locus_tag="VFV55_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4943492.1"
                     /translation="MGVSGRIARYFQDSQLTPLVALVAFLLGLFAVAITPREEEPQIN
                     VTMANVFIPFPGASVQDVESLVSIPAEQVLSQMEGIEHVYSVSRPGMSVLTVQFRVGV
                     PRIDAIVRLRDTMSANRDWAPPELGILEPVIKPRGIDDVPVVSLTLWSDDAGRGAFEL
                     ERVAHAIEVELKRVAGTREVQTLGGPGRVVRVLLDADRLASHGVTALDVRNALQLSNV
                     TLPTGKLVRDNREIAVETGNYLESAADVRALVVGVQEKSPVYLADVAQVVDGPDQPSR
                     YVWTGTGPASDELAPATRGEHPAVTLQITKKPGENAVDVAARVIQRVGELKGTLIPAD
                     MHATVTRNYGATANDKAQSLIKKLVFATLSVVVLVFATLGWREALIVGLAVVLTLAST
                     LFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHRGLKGGPLSGIIPDAVDE
                     VGGPTILATFTVIAALLPMAFVTGLMGPYMSPIPINASMGMVLSLAIAFTVTPWLALK
                     LLATQGHAHASSLDAKLERFFGSLLGRFITANDAARNRRRLGLGIAVAIALSVGLAGV
                     KLVVLKMLPFDNKSEFQVVVDMPTGTALEETSRVLHELGAYLATVPEVTDY"
     gene            complement(1865..2926)
                     /locus_tag="VFV55_04015"
     CDS             complement(1865..2926)
                     /locus_tag="VFV55_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4943493.1"
                     /translation="MKPASLALLAAVGLVAAACGSSAPPGAPPATAVALATAEAQLRE
                     VDLDTTAEAVIEAVRQSTVSAQVSGRIVDIRFDVGDRVEKGAVILRIDERAANQAVAA
                     SEAQVREAEASLANARAQFERTKQLVAQKFVSQAALDKSEADFKAAESRMKAMLAGAG
                     AAATERSFTTIVAPYSGIVSARHVQLGEMAAPGKPLLTGFDPSSLRVVATVASAQVAA
                     IQAGARARIEVPSLDRWVEVRSVTVVPSADPRTHTTQVRLDLPIDATGLLPGVFARAH
                     FATGKAPRLMVPRTAVLRRSEVTAVYVVDGKGVPQLRQVRIGSAADEKSVEILAGLRA
                     GEKVAIEPVKAGMATGAAG"
     gene            complement(2940..3383)
                     /gene="trxC"
                     /locus_tag="VFV55_04020"
     CDS             complement(2940..3383)
                     /gene="trxC"
                     /locus_tag="VFV55_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX4943494.1"
                     /translation="MDATATEFVHVACPKCLSQNRIDAARVTEGPKCGKCETPLLDAS
                     VVELDEASFDAFVARTSVPVLVDFWAPWCGPCKQMAPEFAQAAKRLAGKARLVKVNTD
                     LAQALAGRFAVRSIPTLLLMKEGREAGRKTGARPAADLERWVASA"
     gene            complement(3393..3611)
                     /locus_tag="VFV55_04025"
     CDS             complement(3393..3611)
                     /locus_tag="VFV55_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX4943495.1"
                     /translation="MNTERLVRIFAGAFVLLSLGLGVPGSPMFASQNFLWFTAFVGAN
                     LLQSGFSCFCPLEIILRKLGVKSAAQAA"
     gene            3743..4027
                     /locus_tag="VFV55_04030"
     CDS             3743..4027
                     /locus_tag="VFV55_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEX4943496.1"
                     /translation="MPTIRDETHKKELIHRLRRVEGQLRGIQAMIEKGSDCEAITQQL
                     SASRRALDKAFYHVLSCAIQSGGEAPAGAAGKAVVDKRLERAAELLAKFG"
     gene            4080..>5467
                     /locus_tag="VFV55_04035"
     CDS             4080..>5467
                     /locus_tag="VFV55_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX4943497.1"
                     /translation="MEPSFAPIGREALFDRLALGHAGRLTVLTPNRRLAQALSRAFDD
                     SRARAGLVAWESADIVAWSGFVERLWDESLHAPHGGSLPLLLDPEEERALWESVVGDS
                     RQAKELLSATGPAAQCGEAWQLLHAWRLGPAAASADANEDAKAFHEWAARYERLTRDQ
                     RFVEAARLPDLLVPFVRDGSLRLPATLVLYAFDLLAPQARDLVDAFAAAGVEVLACDA
                     PRHEGHAVRESLATPGDEIAAAARWARARLHEAATRPDAAAPRIGVVVPDLARSRAVV
                     ERVFAATLHPGHNAPGGVRAPRAFGLSLGMPLSGFAIVNDALLVLALAGREIAFEEAS
                     RFLRSPFLAGATVEMAARARLDAALRKRAGVALTLDRLAGLVAAKGMPRAPVLGNLLA
                     GLAEFRRSNLFGMRTPSDWAKAFAQALRLAGFPGDRSLDSAEFQTLGKWHEVVAAFAR
                     LERVAGRMGYREA"
BASE COUNT          802 a         1867 c         1975 g          823 t
ORIGIN      
        1 gatagtcggt cacctcgggc accgtggcaa gataggcacc cagctcgtgc agcacgcgtg
       61 aggtctcctc cagcgctgtg ccggtgggca tgtccaccac cacctggaac tccgacttgt
      121 tgtcgaaggg cagcatcttc agcaccacga gcttcacgcc ggcaaggccc acggacagcg
      181 caatggccac ggcgattccc agccccagcc ggcggcggtt ccgcgcggcg tcgttcgcgg
      241 tgatgaagcg cccgagcagg gaaccgaaga agcgctcgag cttcgcgtcg agggaggagg
      301 cgtgcgcgtg gccctgcgtg gcaaggagct tgagggcgag ccacggcgtc acggtgaagg
      361 cgatcgcgag cgacagcacc atgcccatgc tcgcgttgat ggggatcggg ctcatgtagg
      421 gacccatgag gccggtgacg aacgccatcg gcaggagggc ggcgatgacc gtgaaggtcg
      481 cgaggatcgt ggggccgccc acctcgtcca ccgcatcggg gatgatgccg gagagaggcc
      541 cgcccttgag gccgcggtgg cgatggatgt tctcgaccac cacgatcgcg tcgtcgacca
      601 ggatgccgat ggaaaatatg agcgcgaaga gcgaaacccg gttgagcgtg aatccccacg
      661 cccacgaggc gaagagcgtc gaggcgagcg tgagcaccac ggcgaggccc acgatgagcg
      721 cctcgcgcca gccgagcgtg gcgaagacga gcaccacgac ggacagggtg gcgaacacca
      781 gcttcttgat gaggctttgc gccttgtcgt tggccgtcgc cccgtagttg cgcgtgacgg
      841 tcgcgtgcat gtcggcgggg atgagcgtgc ccttgagttc cccgacgcgc tggatgaccc
      901 gtgcggcgac atccacggcg ttctcgccgg gcttcttggt gatctgcagg gtcacggccg
      961 ggtgctcgcc gcgggtagcg ggcgccaatt cgtccgatgc ggggccggtt cccgtccaga
     1021 cgtagcgcga gggctggtcg gggccgtcga cgacttgcgc cacgtcggcg aggtagacgg
     1081 ggctcttttc ctgcacgccc accacgagcg cgcggacgtc cgccgcggac tccaggtagt
     1141 tgccggtctc cacggcgatc tcgcggttgt cgcgcacgag ctttccggtg ggcaacgtca
     1201 cgttcgaaag ttgcagcgca ttgcgcacgt cgagcgcggt gacgccgtgg gaggcgaggc
     1261 ggtccgcgtc gaggagcacg cgaacgacgc gtcccgggcc gcccagcgtc tgcacctcgc
     1321 gcgtgccggc aacgcgcttg agctccacct cgatcgcgtg cgccacgcgc tcgagctcga
     1381 acgcaccccg gcccgcgtcg tcgctccaga gggtgaggga caccacgggc acgtcgtcga
     1441 tgccgcgggg cttgatgacc ggttcgagga tcccgagttc cgggggcgcc cagtcgcggt
     1501 tggcggacat ggtgtcgcgc agccgcacga tggcgtcgat gcgcggcacg cccacccgga
     1561 actgcacggt gagcaccgac atgccggggc gggagactga gtagacgtgc tcgatgccct
     1621 ccatctggga gagcacctgc tcggccggaa tggagaccag cgactcgacg tcctgcaccg
     1681 acgcgccggg gaacgggatg aagacgttgg ccatcgtcac gttgatctgc ggttcctcct
     1741 cgcgcggcgt gatcgccacg gcgaagaggc ccagcaggaa cgccaccagc gcgacgagcg
     1801 gggtgagctg cgaatcctgg aaatagcgtg cgatgcgccc ggagacaccc acggccggcc
     1861 gcccctagcc cgcggcgccg gtagccatgc cggccttcac cggctcgatg gcgaccttct
     1921 cgcccgccct gaggccggcg agaatctcca ccgacttctc gtcggctgcg gaaccgatcc
     1981 gtacctggcg caattgcggc acgcccttgc cgtcgacgac gtaaacggcg gtgacctcgc
     2041 tgcggcgcag caccgcggtg cgcggaacca tgaggcgcgg ggccttcccc gttgcgaagt
     2101 gggcgcgcgc gaagacgccg gggagcagcc ccgtggcatc gatcggcagg tcgaggcgca
     2161 cctgcgtcgt gtgcgtgcgc gggtcggcgg acgggaccac ggtgacggat ctcacctcga
     2221 cccagcggtc gagggaaggc acctcgatgc gcgccctggc cccggcctgg atggcggcca
     2281 cctgggcgga ggcgacggtg gccacgacgc gaaggctcga cgggtcgaat ccggtaagga
     2341 gcggcttgcc gggcgcggcc atctcgccca gctgcacgtg tcgcgccgag acgatgccgc
     2401 tgtagggagc gacgatggtg gtgaagcttc tttcggtggc ggccgcgccg gcgccggcga
     2461 gcatggcctt catgcgcgat tcggcggcct tgaaatcggc ctcggacttg tcgagggcgg
     2521 cctggctcac gaacttctgc gcgacgagct gcttcgtgcg ctcgaactgc gcgcgggcgt
     2581 tggcgagtga ggcttcggct tcgcgtacct gcgcctcgct cgcggccacg gcctggttcg
     2641 cggcgcgctc gtcgatgcgc aggatcaccg cgcccttctc gacgcggtcg ccgacgtcga
     2701 accggatgtc gacgatgcgc cccgatacct gcgccgagac cgtcgattgc cgcaccgcct
     2761 cgatcacggc ctccgcggtg gtgtcgaggt cgacttcgcg caattgcgcc tcggcggtgg
     2821 caagggcaac cgcagtggcc ggcggagcgc cgggcggggc cgaggagccg caagcggcgg
     2881 cgaccaggcc gacggcggcg agaagggcga gggaggcagg tttcatcacg tgcccgggat
     2941 caggcggaag cgacccagcg ctcgagatcg gcggcggggc gcgccccggt cttgcggccc
     3001 gcttcgcggc cctccttcat gaggagcagg gtcggaatcg agcgcacggc gaatcggccg
     3061 gccagggcct gcgcgaggtc ggtgttcacc ttcaccagcc gcgccttccc cgccaggcgc
     3121 ttggcggcct gcgcgaactc gggggccatc tgcttgcacg gcccgcacca cggcgcccag
     3181 aaatcgacca gcaccggcac gctggtgcgc gcgacgaagg catcgaacga ggcctcgtcc
     3241 agttccacga ccgaagcgtc gaggagcggc gtctcgcact tgccgcattt cgggccctcg
     3301 gtcacgcgcg ccgcatcgat gcggttctgc gagaggcact tcgggcaggc gacgtggacg
     3361 aactcggtgg cggtggcatc catggctttc tcctatgcgg cttgcgcggc gctcttgacg
     3421 ccgagcttcc tcagtatgat ctcgagcggg cagaagcagg agaagccgct ctgcagcagg
     3481 ttggcgccga cgaaggcggt gaaccagagg aaattctggc tcgcgaacat cgggctgccg
     3541 ggcacgccga ggccgaggct cagcaggacg aaggcgccgg cgaagatgcg gacgagtcgt
     3601 tcggtgttca tggcggtctc caggtggggg atggccgcaa tataccatgg ggggtatggt
     3661 atgtcaattg ccggcgcgca tcccgggcgc ttgtctccgg atacccggta gtgtataaac
     3721 ggtgcgatta caggagagtg ccatgcccac catccgggac gaaacgcaca agaaggaact
     3781 gatccaccgg ctgcgccgcg tcgaggggca gttgcgcggc atccaggcga tgatcgagaa
     3841 gggctcggac tgcgaggcca tcacgcagca gctctcggcc agccgccgcg ccctcgacaa
     3901 ggctttctac cacgtgcttt cctgcgccat ccagtcggga ggcgaggccc ccgcgggggc
     3961 cgccggcaag gcagtcgtgg acaagcgcct cgagcgcgcc gcggagctcc tcgccaagtt
     4021 cggctgatgc cggctcccgg gctcatcgcc tgagcctgcc ggccgctatc atgcggccta
     4081 tggaaccctc cttcgcgccc atcggccgcg aggcgttgtt cgatcgcctc gccctcggcc
     4141 acgccgggcg ccttaccgtc ctcaccccca atcgccggct cgcgcaggcg ctctcgcgcg
     4201 ccttcgacga cagccgtgcg cgtgccggcc tcgtcgcctg ggaaagcgcc gacatcgtgg
     4261 cgtggtccgg cttcgtcgag cggctctggg acgagtccct gcatgccccg catggcggca
     4321 gcctgccgct gcttctcgat ccggaggagg agcgggcgtt gtgggagtcg gtggtcggcg
     4381 actcccgcca ggcgaaggaa ctcctttccg ccaccggtcc cgcggcgcag tgcggcgagg
     4441 cctggcaact gctgcacgcg tggcgactcg gccccgccgc ggcatcggcg gacgccaacg
     4501 aggacgcgaa ggcgttccac gaatgggcgg cgcgctacga gcggctcacc cgtgaccagc
     4561 gattcgtgga ggccgcccgc ctgccggacc tcctggttcc cttcgtgcgc gatggctcgc
     4621 ttcgcctgcc ggcaacgctc gtcctttatg ccttcgacct gcttgcgccg caggcgcgcg
     4681 atcttgtcga tgcgttcgct gcggccgggg tcgaggtgct cgcgtgcgat gcgccgcggc
     4741 acgagggcca cgcggttcgc gaatccctcg cgaccccggg cgacgagatc gccgccgcgg
     4801 cccgctgggc acgcgcaagg ctgcacgagg cggcgacgcg gccggatgct gccgcgccgc
     4861 gcatcggcgt cgtggtgccc gatcttgctc ggtcgcgcgc ggtagtcgag cgcgtattcg
     4921 ccgcgacgct gcatcccggc cacaacgcgc ccggcggcgt gcgcgcgccg cgcgcgttcg
     4981 gcctttcgct cggcatgccg ctttccggtt tcgcgatcgt caacgatgcg ctgctcgtcc
     5041 tcgcgctcgc cgggcgcgag atcgcgttcg aggaggcgag ccggttcctg cgctcgccgt
     5101 tccttgccgg cgctacggtc gagatggctg cgcgcgcccg gctggatgcg gcgctgcgca
     5161 agcgcgcagg cgtcgcactc accctggacc ggctcgcggg cctcgttgcg gcgaaaggca
     5221 tgccgcgcgc gcctgttctc ggcaacctcc tcgccgggct cgccgaattc cgccgcagca
     5281 acctcttcgg catgcgcacg ccgtccgact gggcgaaggc gttcgcgcag gccctgcggc
     5341 tcgcgggctt ccccggcgac cgcagcctcg attccgcgga gttccagacc ctcggcaagt
     5401 ggcacgaagt ggtcgcggcc ttcgcccggc tcgagcgggt ggcgggaaga atgggttacc
     5461 gcgaagc
//
LOCUS       DASUEV010000123         5540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_37346, whole genome shotgun sequence.
ACCESSION   DASUEV010000123 DASUEV010000000
VERSION     DASUEV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37346"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1913)
                     /locus_tag="VFV55_04040"
     CDS             complement(<1..1913)
                     /locus_tag="VFV55_04040"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX4943498.1"
                     /translation="MNSAIILALACAALALVYGAVSARWILAQPDGNDRMREIAAAIQ
                     AGAKAYLNRQYGTIGMVGVVLFVVIWLLLGWMTALGFAIGAIASGAAGYIGMNVSVRA
                     NVRTAEAARRGIGPALNIAFKGGAVTGLLVVGLGLLSVTAFFLLLQGSAGLDAKMSDV
                     VKPLIGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTIIATMVLGALLMSSPAVVGNVVTYPLVLGAVSIVASI
                     IGVGFVKTTEGGKIMSALYRGLVVAGVISLVAFYFVTGWMMKDVVAAYPNLTVVNLWG
                     ASVVGLVLTALMVIITEYYTGTDFGPVQHVAEASTKGHATNIIAGIGVSMRSTAWPVL
                     SVCLAIWAAYALAGLYGIAIAATSMLSMAGIIVALDAYGPITDNAGGIAEMAELPKEV
                     RNVTDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLEELGRHITFELSNPAVI
                     IGLFLGGLIPYLFGAMAMEAVGRCAGSVVEEVRRQFREIKGIMEGTAKPDYSRAVDML
                     TKGAIKEMMIPSLLPVLSPVAVALIVGFLMGKDAGVQALGGMLMGTIVTGLFVAISMC
                     TGGGAWDNAKKYI"
     gene            complement(1973..3232)
                     /locus_tag="VFV55_04045"
     CDS             complement(1973..3232)
                     /locus_tag="VFV55_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX4943499.1"
                     /translation="MPRNAFYAQSGGVTAVINASAAGVIETARKHRTKIGKVYAGRDG
                     IIGALTEDLIDTSRESPANIRALMHTPGGAFGSCRYKLKGVEKGAHEYERLIEVFKAH
                     DIGYFLYNGGNDSADTCLKVSRIAAEMGYPLIAVHVPKTIDNDLPVTDTCPGFGSVAK
                     YVATSMREAGFDVASMAKTSTKVFVLEVMGRHAGWITAAVGLAQSKPDEAPHVLLFPE
                     IAFDEAAFLAKVDDSVKRFGYCAIGVSEGLQDRDGRFLSETGLKDAFGHSQLGGVAPV
                     VAGLVQSRLGYKYHWAVADYLQRSARHIASKTDLEQSYAVGKAAVELAIRGESGVMPT
                     IVRVSSRPYRWKIGVARLEDVANVEKKMPRDFIAPDGFHITARCRRYLEPLIAGEAFP
                     PFEKGLPKYVRLKNAAVRKKTKTAFKL"
     gene            complement(3307..3960)
                     /gene="adk"
                     /locus_tag="VFV55_04050"
     CDS             complement(3307..3960)
                     /gene="adk"
                     /locus_tag="VFV55_04050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX4943500.1"
                     /translation="MRLILLGGPGAGKGTQAGYITQKYSIPQISTGDMLRAAVKAGTP
                     RGLAAKAIMDRGELVSDDIIVELVKERIQAPDSTRGFLFDGFPRTIPQAEAMKASGID
                     VDHVVEIAVDDAAIIERMSGRRVHLASGRTYHAKYNPPKVPGKDDVTGEALIQRDDDT
                     EETVRKRLAIYHRQTVPLVEFYAKWAASGDARAPKCHRIPGSGTVEEVRDRVFAALG"
     gene            complement(4059..4853)
                     /gene="kdsB"
                     /locus_tag="VFV55_04055"
     CDS             complement(4059..4853)
                     /gene="kdsB"
                     /locus_tag="VFV55_04055"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX4943501.1"
                     /translation="MVGERAGRIEAFKVIIPARLRSTRLPGKMLAEVAGKPMLAWVAE
                     RAARSGAAEVIIATDHADIADAMTALGWRVCMTAVAHPTGTDRLAEAVRILGLDDGEI
                     VVNVQGDEPLIDPLLVRAVAGELGRRPAASMATAAHPITSANAFFDPNVVKVVLDDEG
                     CATYFSRAPVPYARDAFAASKDVLPPGFPALRHIGIYAYRVEYLRRFSSLSQTPAERF
                     EALEQLRALGHGHRIAVALWTGPVEAGVDTAQDLERVRAILGPARA"
     gene            complement(4856..5035)
                     /locus_tag="VFV55_04060"
     CDS             complement(4856..5035)
                     /locus_tag="VFV55_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX4943502.1"
                     /translation="MDPKLLEILVCPICKGPLVRRREPDELICRADRLAFPVRDDIPV
                     MLEEEARKLGPDEEA"
     gene            complement(5035..>5540)
                     /gene="lpxK"
                     /locus_tag="VFV55_04065"
     CDS             complement(5035..>5540)
                     /gene="lpxK"
                     /locus_tag="VFV55_04065"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX4943503.1"
                     /translation="ERGFGNGLPLPSGPLREPVSRLKSVDAVVVNGGWPERLPAPRQF
                     AMALGGECFVSFSGEELSPPDFALASRGRRVAAVAGIGNPARFFAHLGRLGVVADRHP
                     FPDHHHFQPAELRLPRAELVVMTEKDAVKCAAFADARMWFLRVEAVLPPEFDAFLLER
                     LATGTRR"
BASE COUNT          891 a         1867 c         1914 g          868 t
ORIGIN      
        1 tcgatgtact tcttggcgtt gtcccacgcg ccgccgcccg tgcacatcga gatcgcgacg
       61 aagaggccgg tcacgatcgt tcccatgagc atgccgccga gcgcctggac gcccgcgtcc
      121 ttgcccatca ggaagccgac gatgagggcc acggcgaccg gcgacagcac cggcagcagc
      181 gacgggatca tcatctcctt gatcgcgccc ttggtgagca tgtcgaccgc gcgcgagtag
      241 tcgggcttgg ccgtgccttc catgatcccc ttgatctcgc ggaactggcg gcgcacttcc
      301 tccaccaccg aacccgcgca gcggcccacc gcctccatcg ccatggcgcc gaaaaggtag
      361 gggatgagtc cgccgaggaa caggccgatg atcacggccg ggttggaaag ctcgaacgtg
      421 atgtgccggc ccagctcctc cagcttgtgg gtgtagtcgg cgaagagcac cagggccgcg
      481 aggcccgcgg agccgatggc gtagcccttg gtcaccgcct tggtggtgtt gcccacggcg
      541 tccagcgggt cggtcacgtt gcgcacttcc ttgggcagtt ccgccatctc ggcgatgccg
      601 ccggcgttgt cggtgatcgg gccgtaggcg tcgagcgcca cgatgatgcc ggccatggag
      661 agcatggagg tcgcggcgat ggcgatgccg tacaggccgg cgagcgcgta ggccgcccag
      721 atcgcaaggc acaccgacag caccggccag gcggtcgagc gcatggacac gccgatgccc
      781 gcgatgatgt tggtggcgtg gcccttggtc gaggcttccg cgacgtgctg caccgggccg
      841 aaatcggtgc cggtgtagta ctcggtgatg atcaccatca gcgcggtgag cacgagaccc
      901 acgacggagg cgccccagag gttgaccacc gtgaggttcg ggtaggcggc caccacgtcc
      961 ttcatcatcc agccggtgac gaagtagaag gccaccagcg agatcacgcc ggcgacgacg
     1021 aggccgcgat acagggcgga catgatcttg ccgccctcgg tcgtcttcac gaatccgacg
     1081 ccgatgatcg aggcgacgat cgacacagcg cccagcacca gcggataggt gaccacgttg
     1141 cccacgacgg ccgggctgga catgaggagc gcgcccagca ccatggtcgc gatgatcgtc
     1201 accgcgtagg tctcgaagag gtccgcggcc atgccggcgc aatcgcccac gttgtcgccc
     1261 acgttgtcgg cgatcaccgc ggggttgcgc gggtcgtcct cgggaatgcc ggcctccacc
     1321 ttgcccacga ggtcggcgcc cacgtcggcg cccttggtga agatgccgcc gccgagacgc
     1381 gcgaagatgg agatgaggga cgagccgaag gccaggccga tgagcggctt cacgacgtcg
     1441 ctcatcttcg catcgagccc cgccgagccc tgcagcagaa ggaagaacgc cgtgaccgag
     1501 agcaggccca ggccgaccac caggagcccg gtgacggcgc cgcccttgaa ggcgatgttg
     1561 agcgccgggc cgatgccgcg gcgcgccgcc tcggcggtgc gcacattggc gcgaaccgac
     1621 acgttcatgc cgatgtagcc cgccgcgccc gaggcgatcg cgccgatggc gaagcccagc
     1681 gcggtcatcc agcccagcag gagccagatc accacgaaga gcacgacgcc caccatgccg
     1741 atggtgccgt actggcggtt gaggtaggcc ttcgccccgg cctggatggc cgctgcgatc
     1801 tcgcgcatgc ggtcgttgcc gtccggctgc gcgaggatcc accgggccga gaccgcgccg
     1861 tagaccagcg cgagcgccgc gcaggccagc gccaggatga ttgctgagtt caacatctca
     1921 cttctccctt tgactgcggt tatttgcgac ggaaaagcat gcgccgcccg cgctagagct
     1981 tgaaggcggt cttcgttttc ttgcggaccg cggcgttctt cagccgcacg tacttcggca
     2041 ggcccttctc gaacggggga aacgcctcgc ccgcgatgag cggctcgagg tagcggcggc
     2101 acctggcggt gatgtggaac ccgtccggtg cgatgaaatc gcgcggcatc ttcttttcca
     2161 cgttggcgac gtcctcgagc ctcgccacgc cgatcttcca gcggtaggga cggctcgaaa
     2221 cgcgaacgat ggtgggcatc acgcccgact cgccgcgaat cgccagttcc accgccgcct
     2281 tgcccacggc gtaggactgc tcgaggtcgg tcttcgaggc gatgtggcgc gccgaacgct
     2341 gcaggtaatc ggccaccgcc cagtggtact tgtagccgag gcgcgactgc acgagcccgg
     2401 ccaccaccgg cgccacgcca ccgagctggg agtgcccgaa ggcgtccttg aggcccgtct
     2461 cggagaggaa tcttccgtcc ctgtcctgca gcccctcgga aacgccgatg gcgcagtagc
     2521 cgaagcgctt cacgctatcg tccaccttcg cgaggaaagc cgcctcgtcg aaggcgatct
     2581 cgggaaagag aagcacgtgc ggcgcctcgt ccggcttcga ttgcgccagc cccacggcag
     2641 ccgtgatcca gcccgcgtgg cgccccatca cctcgaggac gaagaccttc gtggaggtct
     2701 tggccatcga ggccacgtcg aatcccgcct cgcgcatgct ggtggccacg tacttggcca
     2761 ccgacccgaa gccggggcag gtgtcggtga cgggcaggtc gttgtcgatc gtcttgggca
     2821 cgtgcacggc gatgagcgga tagcccatct cggcggcgat gcgcgagacc ttgaggcagg
     2881 tgtccgcgga atcgttgccg ccgttgtaga ggaaatagcc gatgtcgtgc gccttgaaga
     2941 cctcgatcag gcgctcgtac tcgtgagcgc ccttctccac gcccttgagc ttgtagcggc
     3001 aggagccgaa ggccccgccg ggcgtgtgca tcagcgcgcg gatgttcgcg ggcgattcgc
     3061 ggctggtgtc gatgaggtct tccgtgagcg cgccgatgat gccgtcgcgt cccgcataga
     3121 ccttgccgat tttcgtgcgg tgcttgcgcg cggtttcgat gacacccgcg gcgctcgcgt
     3181 tgatcaccgc ggtgacgccg cccgactggg catagaacgc gtttctgggc atgggcgcag
     3241 ggagttcagt tggcggttga tcggaacacg gaggtcacgg aggactcgga gttgaatcgc
     3301 cttctcttat cccagcgcgg cgaagacgcg gtcgcggact tcctcgaccg tgccgctgcc
     3361 ggggatgcgg tggcacttgg gcgcgcgtgc gtcgcccgag gcggcccact tggcatagaa
     3421 ctcgacgagc gggacggtct gccggtggta gatggcgagg cgcttgcgca ccgtctcttc
     3481 ggtgtcgtcg tcgcgctgga taagggcttc gcccgtgacg tcgtccttgc ccggcacctt
     3541 cggcgggttg tacttcgcgt ggtaggtgcg gcccgacgcc aggtgcacgc gccgccccga
     3601 catgcgctcg atgatggccg catcgtccac ggcgatctcc acgacgtggt cgacgtcgat
     3661 gccggaggcc ttcatcgctt cggcctgcgg gatggtgcgc gggaagccgt cgaagaggaa
     3721 gccgcgcgtg ctgtcgggcg cctggatcct ctccttcacg agttccacga tgatgtcgtc
     3781 ggaaacgagc tcgccgcggt ccatgatggc cttggctgcc aggcccctcg gcgtaccggc
     3841 cttcacggcg gcgcgaagca tgtcgcccgt cgagatctgt ggaatcgagt atttctgcgt
     3901 gatgtagccg gcctgcgtgc ccttgccggc gcccgggcca cccaacaaga tcaggcgcat
     3961 gggcctgcct ccctcaaggg ttctctgcgg aaaagcgccc gattatcgcc gcctcggggg
     4021 aagcgggtca aacccgggga aatcccgagg gggggtattc aggccctcgc cgggccgagg
     4081 atcgcgcgca cccgctccag gtcctgcgcg gtgtccacgc ccgcctcgac aggcccggtc
     4141 cacaaggcga ccgcgatccg gtggccgtgg cccagggcgc gcagttgctc cagcgcctcg
     4201 aagcgctcgg cgggtgtctg cgacaggctc gaaaaccggc gcaggtattc cacgcggtag
     4261 gcatagatgc cgatgtggcg aagcgccggg aagccgggcg gcagcacgtc cttcgaggcg
     4321 gcgaaggcat cgcgcgcata gggaacgggc gcccgcgaga aataggtcgc gcacccctcg
     4381 tcatcgagga ccaccttcac gacgttcggg tcgaagaagg cattcgccga cgtgatgggg
     4441 tgagcggccg tggccatgga ggccgcgggc cgccttccca gctcgcctgc caccgcgcgg
     4501 acgaggagcg ggtcgatgag gggctcgtca ccctgcacgt tgaccacgat ctcgccatcg
     4561 tcgagcccga ggatccggac ggcctccgcg aggcggtccg ttcccgtggg atgcgcgaca
     4621 gccgtcatgc acacgcgcca gccgagcgca gtcatcgcat cggcgatgtc ggcgtggtcg
     4681 gtcgcgatga tcacttccgc ggcgccgctc cgcgcggccc gctcggccac ccaggcgagc
     4741 atcggctttc cggcgacctc ggcgagcatc ttgcccggca gtcgcgtcga gcgaagccgc
     4801 gccgggatga tgaccttgaa ggcctcgatc ctgccggccc tttccccgac cacggtcagg
     4861 cttcctcgtc gggaccgagc ttcctcgcct cctcctcgag catgaccggg atgtcgtcgc
     4921 gtaccgggaa ggccagccgg tcggcgcggc agatgagctc gtcgggttcg cggcggcgga
     4981 cgagcgggcc cttgcagatc gggcagacga ggatctcgag gagcttgggg tccatcagcg
     5041 gcgtgtcccg gtggcgaggc gttcgaggag gaaggcgtcg aactccggcg gcagcaccgc
     5101 ctcgacgcga aggaaccaca tccgcgcatc cgcgaatgcc gcgcatttta cggcatcctt
     5161 ctcggtcatg acgaccagtt ccgcgcgcgg caggcgcagt tctgccggct ggaagtggtg
     5221 atgatcggga aacgggtggc gatcggccac cacgcccagc cgcccgaggt gggcgaagaa
     5281 ccgcgcggga ttgccgatgc ccgccaccgc cgcgacgcga cgcccgcgcg aggcgagcgc
     5341 gaaatccggc ggggacagct cctcgccgga gaacgaaacg aagcattctc caccgagggc
     5401 catcgcgaac tggcgcggcg cgggaagccg ctcgggccag ccgccgttca ccaccaccgc
     5461 atcgacgctc ttcaggcgcg acaccggctc gcgaagcggt cccgagggca gcggcaaccc
     5521 gttgccgaag ccgcgctcgc
//
LOCUS       DASUEV010000124        11566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_838891, whole genome shotgun sequence.
ACCESSION   DASUEV010000124 DASUEV010000000
VERSION     DASUEV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838891"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..179
                     /locus_tag="VFV55_04070"
     CDS             27..179
                     /locus_tag="VFV55_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX4943504.1"
                     /translation="MCLVCGYVYDEAEGSPDDGLAPGTRWEDVPANWSCPECGARKDD
                     FEMVEL"
     gene            437..802
                     /locus_tag="VFV55_04075"
     CDS             437..802
                     /locus_tag="VFV55_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4943505.1"
                     /translation="MVIDDSNTIRRSAEIFLMQAGCQVILAENGFDALAKIADHEPDL
                     VFVDIMMPRLDGYQTCALIKKSGKHHATPVVMLSSKDSLFDRARGRMVGSDEYLTKPF
                     TKESLLRAVETHVARRSTA"
     gene            836..1201
                     /locus_tag="VFV55_04080"
     CDS             836..1201
                     /locus_tag="VFV55_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4943506.1"
                     /translation="MPIKKVMVVDDSPTERAYLDNLLKKKGFEVILVDSGEAAIERAK
                     SDRPDLILMDVVMPGLNGFQATRAITRDESTKHIPVIICTTKDQETDKIWGLRQGAKD
                     YVTKPINEADLIEKIKSFG"
     gene            1215..1730
                     /locus_tag="VFV55_04085"
     CDS             1215..1730
                     /locus_tag="VFV55_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX4943507.1"
                     /translation="MARRTGLREFQLSVAERLRTAATRATVSSKLGFQIGGVNWFVSL
                     EQASEVIPLPALVPVPLTQPWFRGVANVRGKLYGIVDFNAFQGGEPTPGGTERRVILV
                     SDKLVSGAGILVSRMLGLRNPEHFTAAERPADAAAWVGAVFTDASGASWRELDLARLA
                     KERRFLEVGIH"
     gene            complement(1727..2068)
                     /locus_tag="VFV55_04090"
     CDS             complement(1727..2068)
                     /locus_tag="VFV55_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943508.1"
                     /translation="MFICVVIGMWPIRGTLKLSRLRRRRAAAAGFLAIAPPIVEGGPP
                     GRVSGPPPAVNLVIVDDAAEAGRAPAAGFGAPIFRNQSASEVLSPAAGALAMVFSVQR
                     DIEGGADARTP"
     gene            2043..4040
                     /locus_tag="VFV55_04095"
     CDS             2043..4040
                     /locus_tag="VFV55_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX4943509.1"
                     /translation="MPITTQMNIMGTIALGLVALTALMVFVDATARTRNSTYVTVAAQ
                     MQYHTQRLAKAASLAARGQQAAFPQVQDSRDEFANFLKVLQEGGFAFGIEVPSAATSE
                     ELRSRLEELGQRWPESSNAATAILAARSDLVSLAQNIAQIRVGAEEMASISQEFTALM
                     GQGGSVPGQVLRASRLTFLAERLGRGSADILGAETIDPEVPFLIGKDTNDLRDLIKAL
                     ESGSEGLAIVAVRDAEARGKLAELKAQFEKFEKNVGPILRELQKLVSARQAGAQLVAG
                     SEQLQSAVGRLQDALQGEKSAATLVAVFVFGGLLVIVLVVMGLVFLADSRRSAAQAEK
                     ENKKNQEAILRLLNEMGDLADGDLTVKAKVTEDITGAIADSMNYTVDELRTLVTGVNS
                     AAISVTQKTAQAQSISAELLGAAERQSKEIEETTSQVLQVSRSISEVSSTAEEGAQVA
                     QRSLAAADKGRLAVENSISGMNDIREQIQETSKRIKRLGESSQEIGEIVELISDITEQ
                     TNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSGEATKQIGAIVKSIQADTQDAV
                     AAMERSTHGVVEGAKLSDAAGQALNEIDTVTKNLAQLIQQISQATNTQAEATNTVARN
                     MQEILEITRQTTRGTQQTAGSIQDLSTVAQELKSSVAGFKL"
     gene            4047..9653
                     /locus_tag="VFV55_04100"
     CDS             4047..9653
                     /locus_tag="VFV55_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX4943510.1"
                     /translation="MAARPAPGVDLGPLSWVKTEIDHSLGQARENLDRILTAPRDRAP
                     VKYILTHLHQATGALAMVGLGAATRFNEELERLVTSVEACDAAQLGARVPVAKRAISI
                     LSGYLDSLMSGAADRPMSLLPGYLELNRARGASDATEGDLFHPDLGAALPAPAEPAAA
                     MDDAVLAKALTHQRSQYQQGLLRMLRGGDAREAGRVMQGAIAAIESFQAAAASRPFWT
                     AAAGFFDALALDGLVLDAATKPLLAKVDQQVKQLIDGSARVPERLFRDLLLQVGRSRP
                     VSPRIAALKEAYRLDELLARPEPSQGDTADEALGSLVRELRELTVQQKDTWLKFTSGN
                     RAALEPFGKQALALADRAAPLPRGDLRQVLARLADVAPMLKARAIPPSEAQALEVATA
                     LLFIESALENYFRLGADFDRQAATMSERLKAAMAGEAIAPLDTIEGGILDEMTRRAQE
                     RLLVFQVGQEVQVNLQGIEQALDAFFRDPARRDGLRALPAQFAQVQGALRIMELDAAA
                     GLNGAVESRVRQFAEGTADGTGEAAEMVADGLSALGLYITAIQQGAARPEEVLEPALV
                     RLGLIPSLEAVEEEDFRKTGTVSPLDLDVQKQKVQALYEDWKEIPASEAKEKLERAVD
                     DLKRDAVVIADAEVARQSDQALAALQGAADASQSGVFRAIRELAPDKPPEVPPPQVVQ
                     LVDAPGAEIDRELLEIFLEEAAEVAATIAQQLETCRSAPHDRDSLTTIRRGFHTLKGS
                     GRMVGLDDLGEVAWQCEQVLNKWLKEENPATPGLLDFIALAQRSFSGWIAQLKSGSGT
                     SIDGTEIARCAELLKSGAAEAPAPVPESVPVETVAPEGFTFESLELLPPAEAPPPPAA
                     GAEEAEPAVPVPPAAVHEEPAEAIEEPAEEPDVVVGTVSLSFPLYAIYVGESEQHAAT
                     LEREMSAIEADPMHPVSHEFMRAAHTLTSSSRTTGFHVLADVAHALEKWLADAIDYPP
                     EFTAARLATTRRAVDGVISMVRSIAGRALPLGRNDLVEDLKALREGLQESRRTGEGTH
                     IRMPGVIREALEARAAQAQAVPSLPGEPREAIPTAPREVAIPAAPGEELAAPVEEEPV
                     QEAALPFEAGKEQRKIRDDVDRDLLPIFLEEARELVPAVSDGLRRWKANPSDIAATAE
                     LRRYLHTLKGSSRMTGLMRLGELAHVLEARVIEMGTKAAPSLHEFEEAEERIDRFSVA
                     LERLARGEDMQDLVPVEVPVSAVFEQQKDKPTPLAVIAAAAETMARQQGLVPEQREVR
                     AALLRVNADLVDQFVNEAGELSIARSRIEGEIAAFRRALSDMAENVARMRAQLREIEM
                     ASEGQMQSRLKEQEEHGGAFDPLEFDRFTRMQELTRFLAESLSDVITLQQGLQKSIDE
                     TELALLQQSRLNRELQQGLMGVRLVPLANLADRFYRVVRQTAKDLDRKANLELKGIRV
                     ELDRSVLEKITAPFEHLLRNAVAHGIEPPQARLAAGKPEIGEIVIDAVQRGNEIVLTI
                     SDDGRGLDYPLIRSRAVELGMLGANDELPEAALAQYIFWTGFSTAKEVTQVAGRGIGM
                     DVVRNEITSLGGRVELASTPGRGTTFTITLPLTLAVTQAVMLRAGDELYAVPSAMVDQ
                     VQEYKAQSYAEVAARGEVLWKDNRYPLRSLGALLGGHDSPVPPRQIPVLLLKSGVQRA
                     AVRVDEIIGNREVVVKTIGPQLARLAGVSGATVLGNGQVVLILNPVNLVHLEGGAAEA
                     APTAPAPQAPATPAKAAAPLVMVVDDSLTVRKITGRMLSREGYEFVAAKDGVDALQQL
                     QDVRPDVILLDVEMPRMDGFEFARNVRADSATRSIPIIMITSRTADKHRNRALELGVN
                     EYMGKPYQEDQLLALIARYARATTPA"
     gene            complement(9664..10470)
                     /locus_tag="VFV55_04105"
     CDS             complement(9664..10470)
                     /locus_tag="VFV55_04105"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX4943511.1"
                     /translation="MEIAPLVNALVLGVVEGLTEFLPVSSTGHLIIVSDLLGLNDEKG
                     KVFDIVIQLGAILAVCWEYRARFARAFSGVASDPVQQRFATNLVVAFLPAAVIGLAFQ
                     REIKAFLFNPVSVAVALVAGAIVIFAVERWYARHGAPRVVAVDEMRWGDALKVGFAQC
                     FSLIPGTSRSGATIIGGMLFGLSRQAATEFSFFLAVPIMFAATGYQLVKYRHLLDAAD
                     LGPFAVGFAVSFVAALAAVRGLIRYVANHDFRAFAWYRIALGVAVLAYFR"
     gene            complement(10539..>11566)
                     /gene="thiC"
                     /locus_tag="VFV55_04110"
     CDS             complement(10539..>11566)
                     /gene="thiC"
                     /locus_tag="VFV55_04110"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX4943512.1"
                     /translation="VPIYQALEKVDGKAEELTWEIFRDTLVEQAEQGVDYFTIHAGVR
                     LPFIPMTAKRMTGIVSRGGSIMAKWCLAHHRESFLYERFEDICAIMAAYDVSFSLGDG
                     LRPGSIYDANDEAQFAELETLGELTSIAWKHDCQVMIEGPGHVPMQLIKENMDLELEH
                     CHEAPFYTLGPLTTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGLPDKDDVK
                     DGIITYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFNLGLDPDKAKQFHDET
                     LPQEGAKLAHFCSMCGPHFCSMKITQDVREFAARHGVSEQEALAKGMEVKAVEFVAKG
                     AELYSKA"
BASE COUNT         1969 a         3874 c         4055 g         1668 t
ORIGIN      
        1 ccgcccccgc ccggtacaga acctggatgt gcctcgtctg cggctacgtc tacgacgagg
       61 cggagggttc cccggacgac ggcctcgccc caggcacgcg ctgggaggac gtgcccgcga
      121 attggagctg tccggaatgc ggtgcgcgca aggacgattt cgagatggtg gagctgtgag
      181 gtggtgaaac gggttccgcg actgttcgaa acggcttcaa gttgcggcgc ttgaatcttg
      241 ttttcaaggg ccaaatggca taggctacat tcgttaagcg agcagtcgcc aaagagcgaa
      301 ttcgcggggg aggcccggtt cggtcgatgc tgcgtgcgca gaggtgcgtt gcgcgcgcga
      361 gtgccttcgg cgccttgaag cagtgaaaat cgaggaaggc gtggcggaca cgaagaccct
      421 tgccggcgtg cgcgtgatgg tgatcgacga cagcaacacc atccgtcgca gcgccgagat
      481 cttcctcatg caggccggct gccaggtcat cctcgccgag aacggcttcg acgccctggc
      541 gaagatcgcc gaccatgagc ccgacctcgt cttcgtcgac atcatgatgc cgcgactgga
      601 cggctaccag acctgcgcgc tgatcaagaa gagcggcaag caccatgcga caccggtggt
      661 catgctttcg tcgaaggaca gcctcttcga ccgcgcgcgc ggccgcatgg tcggttccga
      721 cgagtacctg accaagccgt tcaccaagga aagccttctc cgggccgtcg agacccacgt
      781 cgcccgccgg agcacggcct gacgaacccg aatcacgtaa tggagtgcta agccaatgcc
      841 gatcaagaaa gtcatggttg tggacgattc gccgacggag cgggcctacc tggacaacct
      901 cctgaagaag aagggattcg aggtgatcct cgtcgacagc ggcgaggcgg cgatcgagcg
      961 cgcgaagagc gatcgcccgg acctcatcct catggacgtc gtcatgcccg ggcttaacgg
     1021 cttccaggcg acgcgggcca tcacccgcga cgagtcgacc aagcacatcc cggtcatcat
     1081 ctgcaccacc aaggaccagg agacggacaa gatctggggc ctgcgccagg gcgccaagga
     1141 ctacgtcacc aagccgatca acgaggccga tctgatcgaa aagatcaagt cgttcggttg
     1201 aggtgccggg ttccgtggcg cgccggaccg gtcttcgcga gttccagctc tcggttgccg
     1261 agcggctgcg cacggccgcg acccgcgcga ccgtgtcgtc gaagctgggc ttccagatcg
     1321 gcggcgtgaa ctggttcgtt tcgctcgagc aggcgagcga ggtcatcccg ctgccggccc
     1381 ttgttccggt gccgctcacc cagccctggt tccgcggcgt ggcgaacgtc cggggaaagc
     1441 tgtacggcat cgtcgacttc aacgcgtttc agggcgggga gccgaccccc ggcggcacgg
     1501 agcgccgggt gatcctcgtc tcggacaagc tcgtgagcgg cgcggggatc ctcgtctcgc
     1561 gcatgctcgg gctgcgcaat cccgagcatt tcaccgccgc cgagcggcct gcggacgccg
     1621 ccgcctgggt gggcgccgtt ttcacggatg catcgggcgc atcctggcgg gaactcgacc
     1681 tcgcgcggct ggcgaaggaa cgccgcttcc tggaggtcgg aatccactag ggcgtgcgcg
     1741 catcggcgcc gccttcgata tcacgctgca cggagaacac catggccaaa gcaccagcgg
     1801 caggactcaa gacttcgctc gcggactggt tccggaagat cggcgcgcca aagccggccg
     1861 cgggcgcgcg gccggcttcc gccgcgtcgt ccacgatgac gaggttgacg gccgggggcg
     1921 gtcccgagac ccgtcccggc ggccccccct cgacgatggg cggcgcgatg gcgaggaaac
     1981 cggctgcggc cgcgcggcgt cgcctgagcc tggacaattt cagcgtgccc ctgatcggcc
     2041 acatgccgat cacgacgcag atgaacatca tgggcacgat cgcgctcggg ctcgtggcgc
     2101 tcaccgcgct gatggtgttc gtggacgcca cggcacgcac gaggaactcg acctacgtga
     2161 cggtggccgc gcagatgcag taccacacgc agcgcctggc caaggccgcg agcctcgcgg
     2221 cccggggcca gcaggcggcc ttcccgcagg tgcaggacag ccgggacgag ttcgccaact
     2281 tcctcaaggt gctgcaggag ggtggcttcg cattcggcat cgaggtgccc tcggcagcca
     2341 ccagcgagga actgaggagc cgcctcgagg agctcggcca gcgctggccg gaatcgtcga
     2401 acgccgccac cgccatcctc gcggcccgaa gcgacctggt ctcgctcgcg cagaacatcg
     2461 cccagatccg cgtcggcgcc gaggaaatgg cctcgatctc ccaggagttc accgcgctca
     2521 tgggccaggg gggctccgtg ccgggccagg tgctccgggc cagccgcctg acgttcctcg
     2581 ccgagcgcct gggtcgcggc tccgccgaca tcctcggcgc cgagaccatc gatccggaag
     2641 tgccgttcct catcgggaag gacaccaacg acttgcgcga cctcatcaag gcgctcgaat
     2701 ccggcagcga agggctcgcg atcgtcgcgg tgcgtgatgc cgaggcgcgc ggcaagctgg
     2761 cggagctcaa ggcgcagttc gagaagttcg agaagaacgt cggcccgatc ctgcgcgagt
     2821 tgcagaagct cgtgtcggcc cggcaggcgg gcgcgcagct cgtggcgggt tccgagcagt
     2881 tgcagagcgc cgtcgggcgc ctccaggacg cattgcaggg cgagaaatcc gcggccacgc
     2941 tggtcgcggt gttcgttttc ggggggttgc tcgtcatcgt tctcgtcgtg atgggcctcg
     3001 tcttcctcgc ggactcccgg cgcagcgccg cgcaggccga gaaggagaac aagaagaacc
     3061 aggaggcgat cctgcgcctg ctcaacgaga tgggcgacct cgccgacggc gaccttacgg
     3121 tgaaggcaaa ggtaaccgag gacatcacgg gcgccatcgc ggattcgatg aactacacgg
     3181 tcgacgagct gaggacgctg gtgaccggcg tgaacagcgc ggccatctcg gtgacgcaga
     3241 agacggccca ggcgcagtcg atttccgcgg aactcctcgg cgccgcggag cggcagtcga
     3301 aggaaatcga ggagacgacc tcccaggtgc tccaggtgtc gcgctcgatt tcagaggtgt
     3361 cgtccactgc cgaggagggc gcgcaggttg cgcagcgttc ccttgcggcc gccgacaagg
     3421 gccgcctcgc ggtggaaaac tcgatctccg ggatgaacga cattcgcgag cagatccagg
     3481 agacctccaa gcggatcaag cgcctcggcg agtcgtcgca ggagatcggc gagatcgtgg
     3541 agctcatttc ggacatcacg gagcagacga acgtgctcgc cctcaatgcc gccatccagg
     3601 cggcatcggc cggcgaggcg ggccgcggct tctcggtggt ggcggaggaa gtgcagcgcc
     3661 tcgcggaacg ctcgggcgag gcgaccaagc agatcggtgc gatcgtgaag tcgatccagg
     3721 cggacaccca ggacgcggtg gccgccatgg agagatcgac gcacggcgtg gtcgaggggg
     3781 ccaagctctc cgacgcggcg ggccaggccc tgaacgagat cgacacggtg acgaagaacc
     3841 tcgcgcagct catccagcag atctcgcagg ccacgaacac gcaggccgag gctaccaaca
     3901 ccgtggcgcg caacatgcag gaaatcctgg agatcacgcg ccagaccacg cgcggcaccc
     3961 agcagacggc cgggtcgatc caggacctct cgacggtggc gcaggaactg aagagttccg
     4021 tcgccggttt caagctctag gaaaccatgg ccgcccgtcc cgcgccaggc gtcgatctcg
     4081 gcccgctcag ctgggtcaag accgagatcg accattcgct ggggcaggca cgcgagaacc
     4141 tcgacaggat cctcactgcg ccgcgggacc gagcgccggt caagtacatc ctcacccacc
     4201 tgcaccaggc cacgggcgcg ctggcgatgg tcgggctcgg ggcagccacc cgcttcaacg
     4261 aggagctgga gcggctcgtg acctcggtgg aggcgtgcga tgccgcgcag ctgggcgcac
     4321 gcgttccggt cgccaagcgg gcgatctcga tcctgtccgg ctatctcgac tccctgatga
     4381 gcggcgcggc cgaccggccg atgtcgctgc tgccgggata cctggagctg aaccgcgcgc
     4441 gcggggcttc cgatgccacc gagggggacc tgttccatcc cgacctgggc gccgcgttgc
     4501 ctgcgcctgc ggagccggct gccgcgatgg acgacgcggt gcttgccaag gcgctcacgc
     4561 accagcgctc ccagtaccag cagggcctgc tgcgcatgct gcgcggtggc gacgcacgcg
     4621 aggccggtcg agtgatgcag ggcgcgatcg cggcgatcga atcgttccag gccgccgcag
     4681 ccagccgtcc cttctggacg gcggcggcgg gcttcttcga tgcgctcgcc ctggatggtc
     4741 tcgtgctcga tgccgcgacg aagcccctgc tggccaaggt ggaccagcag gtgaagcaat
     4801 tgatcgacgg ttccgccagg gtgccggagc gactcttccg cgaccttctg ctgcaggtcg
     4861 ggcgcagccg tcccgtcagc ccgcggatcg ccgcgctgaa ggaggcctat cgcctcgacg
     4921 aactgctcgc gaggccggag ccgtcgcagg gcgacacggc cgacgaggcg ctgggctcgc
     4981 tcgtgcgcga gcttcgcgag cttaccgtcc agcagaagga cacctggctc aagttcacct
     5041 cgggcaaccg cgccgccctg gagccgttcg gcaagcaggc cctggcgctc gccgaccgcg
     5101 ccgcgccgtt gcctcgcggc gacctgcggc aggtgctcgc gaggcttgcg gacgtcgcgc
     5161 ccatgctcaa ggcacgcgcc ataccgccct ccgaagcgca ggccctggag gttgcgacgg
     5221 cactgctttt catcgagtcc gcgctggaaa actatttcag gctgggcgcg gacttcgacc
     5281 ggcaggcggc gacgatgtcg gagcgcctga aggcggccat ggctggcgag gcgatcgcgc
     5341 cgttggacac gatcgagggc ggaatcctcg acgaaatgac gcgacgtgcc caggaaaggc
     5401 tcctcgtctt ccaggtggga caggaagtgc aggtgaacct ccagggaatc gagcaggctc
     5461 tggacgcgtt cttccgcgac cccgcccggc gcgacggcct cagggcgctc cccgcccagt
     5521 tcgcgcaggt ccagggcgcg ctcaggatca tggaactgga cgccgccgcc ggactcaacg
     5581 gcgcggtcga gtctcgcgta cgccagttcg ccgaggggac ggcagacgga acgggcgagg
     5641 ccgccgagat ggttgccgac ggcctgtccg cgctgggcct ttacatcacc gcgatccagc
     5701 agggcgcggc acggccggag gaggtcctcg aaccggcgct ggtgcgcctc gggctcattc
     5761 cctccctcga agcggtcgaa gaggaggatt tccggaagac cggcacggtg tcgccgctgg
     5821 atctcgacgt gcagaagcag aaggtgcagg cgctctacga ggactggaag gagattcccg
     5881 cgagcgaggc gaaggaaaag ctcgagcgcg cggtcgacga cctcaagcgc gacgccgtcg
     5941 tgatcgccga tgccgaggtg gcccggcaaa gcgaccaggc tctcgcggcc ctgcaagggg
     6001 cggccgacgc gagccagtcg ggcgtctttc gcgcgatccg cgagctggcc ccggacaagc
     6061 cgccggaggt gccaccgccg caggtggtgc agctcgtcga tgcccccggc gcggaaatcg
     6121 acagggagct cctcgagatc ttcctcgagg aggcggccga ggtcgccgcg acgatcgctc
     6181 agcagctcga gacatgccga agcgccccgc acgaccgcga ctcgctgacc acgatccggc
     6241 gcgggttcca cacgcttaag ggcagcggcc gcatggtcgg cctcgatgac ctgggcgagg
     6301 tcgcgtggca atgcgagcag gtgctgaaca agtggctgaa ggaggagaat cccgccacgc
     6361 cgggcctgct cgacttcatc gcgctcgcgc agcggtcgtt ctcggggtgg atcgcgcagc
     6421 tgaagtcggg cagcggtacg tcgatcgacg gcacggagat cgcccgctgc gcggaactcc
     6481 tgaagagcgg cgcggcggag gcgcccgcgc cggtgcccga gagcgttccg gtggaaacag
     6541 ttgcgcccga aggcttcacg ttcgagtccc tcgagcttct gccgcccgcc gaggcgccgc
     6601 cgccccctgc ggccggggcg gaagaagccg aacccgcggt gcccgtgccg cccgctgccg
     6661 tgcatgagga gcccgcggaa gcgatcgagg agcccgccga ggaacccgac gtggttgtcg
     6721 gcacggtttc gctgtcgttc ccgttgtacg cgatctacgt gggcgagtcc gagcagcatg
     6781 cggccacgct cgagcgcgag atgtccgcga tcgaggccga cccgatgcac ccggtgagcc
     6841 acgagttcat gcgcgccgcg cacacgctga ccagcagttc ccggacgacc gggttccacg
     6901 tgctggcgga cgttgcgcac gcgctggaaa agtggcttgc cgacgcgatc gactacccgc
     6961 cggaattcac ggccgccagg ctcgccacga cgcgccgcgc ggtggatggc gtcatatcca
     7021 tggtgcggtc catcgccggt cgcgcgcttc cgctcgggcg caacgatctc gtcgaggacc
     7081 tgaaggcgct tcgcgagggg ctccaggagt cgcggcgcac gggagagggc acccacatcc
     7141 gcatgccggg cgtcatccgc gaggcgctcg aggcgcgggc ggcgcaggct caggcggtgc
     7201 cgtcgctgcc cggcgagccc cgcgaggcga ttcccaccgc gccccgcgag gtggcgatac
     7261 ccgccgcgcc cggggaggaa ctcgcggcac ccgtggaaga ggagccggtg caggaagccg
     7321 cgctgccctt cgaagccggc aaggaacagc gcaagatccg ggacgacgtc gaccgcgacc
     7381 tgcttccgat cttcctcgag gaagcccgcg agctcgtgcc cgcggtgagc gacggcctgc
     7441 ggcgctggaa ggcgaaccct tccgacatcg cggccaccgc ggagctgcgc cgctacctgc
     7501 acacgctgaa gggcagctcg cgcatgacgg gcctcatgcg cctgggcgag ctcgcgcacg
     7561 tgctcgaggc gcgagtcatc gagatgggga cgaaggccgc tccgtcgctg cacgagttcg
     7621 aggaggccga ggagcggatc gaccgcttct ccgtcgccct cgagcggctc gcccgcggcg
     7681 aggacatgca ggacctggtg ccggtcgagg ttccggtgtc cgccgtcttc gaacagcaga
     7741 aggacaagcc gacgcctctc gcggtgatcg ccgcggctgc ggagacgatg gccaggcagc
     7801 aggggctcgt acccgagcag cgcgaagtgc gcgcggcact gctgcgcgtg aatgccgacc
     7861 tcgtcgacca gttcgtgaac gaggcgggcg agctctcgat cgcgcgttcg cgcatcgaag
     7921 gcgagatcgc cgccttcagg cgggcgcttt ccgacatggc cgagaacgtg gcgcggatgc
     7981 gcgcgcagct gcgcgagatc gaaatggcct ccgaaggcca gatgcagagc cggctcaagg
     8041 agcaggagga acacggcggc gccttcgatc ccctcgagtt cgaccgcttc acgcgcatgc
     8101 aggagctcac gcgcttcctg gcggaatcgc tctccgacgt gataaccctg cagcagggcc
     8161 tgcagaagag catcgacgag acggagctgg cactcctgca gcagtcccgg ctgaaccgcg
     8221 agctccagca gggcctcatg ggcgtgcggc tcgtgcccct ggccaacctc gcggaccgct
     8281 tctaccgcgt cgtgcgccag accgcgaagg acctcgacag gaaggcgaac ctggagctca
     8341 agggcatacg cgtggagctc gaccgctccg tgctggaaaa gatcacggct cccttcgagc
     8401 acctgctgag gaacgcggtg gcgcacggaa tcgagccccc gcaggcgcgc ctggcggcgg
     8461 gcaagcccga aatcggcgag atcgtgatcg acgccgtgca gcgcggcaac gagatcgtcc
     8521 tcaccatctc cgacgacggc cgcggcctgg attaccccct gatccgcagc cgcgccgtgg
     8581 agctcggcat gctcggggcg aacgacgagc tcccggaggc ggcgctcgcc cagtacatct
     8641 tctggaccgg gttctccacg gcgaaggagg tcacgcaggt ggccggccgc ggcatcggca
     8701 tggacgtggt gaggaacgaa atcacctcgc tcgggggtcg cgtggagcta gcctccacgc
     8761 cggggcgcgg caccacgttc accatcaccc tgcccctcac cctcgcggtg acccaggccg
     8821 tgatgctgcg cgccggggac gagctctacg ccgtgccctc ggcgatggtc gaccaggtcc
     8881 aggagtacaa ggcgcagtcc tatgccgagg tcgccgcccg cggcgaggtg ctctggaagg
     8941 acaatcgcta tccgctgcgc tcgctgggcg cgctgctggg cggacacgac agtcccgttc
     9001 cgccgcggca gattcccgtg ctcctcctga agagcggcgt gcagcgcgcg gcggtgcgcg
     9061 tcgacgagat catcggcaac cgcgaagtgg tggtgaagac catcggcccg cagctcgcgc
     9121 ggctggccgg cgtttccggc gccacggtgc tcggcaacgg gcaggtggtg ctgatcctca
     9181 acccggtgaa cctggtgcac ctcgagggcg gcgcggccga agcggccccg accgcgccgg
     9241 caccgcaggc gcccgcgacg ccagcgaagg ccgcggcgcc gctggtgatg gtggtggacg
     9301 attcgcttac ggtgcgcaag atcaccggcc gcatgctctc gcgcgagggt tacgaattcg
     9361 tcgccgcaaa ggacggggtc gacgcgctgc agcagcttca ggacgtgcgg cccgacgtca
     9421 tcctgctcga cgtcgagatg ccgcgcatgg acggattcga gttcgcgagg aacgtgcgcg
     9481 cggacagcgc cacgaggtcg atcccgatca tcatgatcac ctcgcgcacg gcggacaagc
     9541 accgcaaccg cgccctcgag ctgggcgtga acgagtacat gggcaagccc taccaggaag
     9601 accagctcct tgcgctcatc gcgcgttacg cgcgcgcgac cacgccggcc tgaacgtctc
     9661 ccgctagcgg aagtaggcga gcaccgccac gcccagggcg atgcggtacc acgcgaaggc
     9721 ccggaagtcg tggttcgcca cgtagcggat caggccccgc accgccgcga gcgccgcgac
     9781 gaaactcacc gcgaatccca cggcgaaggg gcccaggtcg gccgcatcca ggaggtgccg
     9841 gtacttgacc agctggtaac ccgtcgcggc gaacatgatc ggcaccgcga gaaagaacga
     9901 gaactcggtc gcggcctggc gcgacaaccc gaagagcatt ccgccgatga tcgtggcacc
     9961 ggagcgcgag gtgccgggga tgagcgagaa gcactgcgcg aaccccacct tgagcgcgtc
    10021 cccccagcgc atttcgtcga ccgcgacgac gcgcggcgcg ccatggcgcg cgtaccagcg
    10081 ctcgacggcg aagatcacga tcgcgccggc cacgagggca acggcgacgc tcaccgggtt
    10141 gaagaggaac gccttgatct cgcgctggaa cgcgagcccg atcaccgccg cggggaggaa
    10201 tgcgacgacc aggtttgtcg cgaatcgctg ctgcaccggg tcggaggcga caccggaaaa
    10261 ggccctggcg aagcgggcgc ggtactccca gcacaccgcg aggatcgcgc ccagctggat
    10321 cacgatgtcg aacaccttgc ccttctcgtc gttcaggccg aggagatcgg aaacgatgat
    10381 gaggtggccg gtggaggaga ccggcaggaa ttccgtgaga ccctccacca cgcccaggac
    10441 gagcgcgttc acgagcgggg cgatttccat cggcgcgagt ctagcatcgc ggcaacggcc
    10501 gggacgcgga cgcgcgcgta aagtggtggc gcggggcctc aggccttcga gtagagctcg
    10561 gcgcccttcg ccacgaactc caccgccttc acctccatcc ccttcgccag ggcctcctgc
    10621 tcggatacgc cgtgcctcgc cgcgaattcg cgcacgtcct gggtgatctt catcgagcag
    10681 aagtgcgggc cgcacatcga gcagaaatgc gcgagcttgg cgccttcctg gggaagcgtc
    10741 tcgtcgtgga actgcttggc cttgtccggg tcgaggccca ggttgaactg gtcgtcccag
    10801 cggaactcga agcgcgcctt cgacagtgca ttgtcgcgga cctgcgcgcc ggggtgtccc
    10861 ttggccaggt cggcggcgtg cgcggcgatc ttgtaggtga tgatgccgtc cttcacgtcg
    10921 tccttgtccg gcaggcccag gtgctccttg ggcgtcacgt agcagagcat ggcggtgccg
    10981 taccagccga tctgcgccgc gccgatgccg ctggtgatgt ggtcgtagcc cggcgcgatg
    11041 tcggtggtca gcgggcccag cgtgtagaac ggcgcctcgt ggcagtgctc gagctcgagg
    11101 tccatgttct ccttgatgag ctgcatcggc acgtggccgg ggccctcgat catcacttgg
    11161 cagtcgtgct tccaggcgat cgaggtgagc tcgccgaggg tctccagctc ggcgaactgc
    11221 gcctcgtcgt tggcgtcgta gatggagccg gggcgcagcc cgtcgcccag cgagaacgag
    11281 acgtcgtagg cggccatgat cgcgcagatg tcctcgaagc gctcgtagag gaagctttcc
    11341 ctgtggtggg cgaggcacca cttggccatg atcgagccgc cgcgcgagac gatgccggtc
    11401 atgcgcttcg cggtcatcgg gatgaagggc aggcgcacgc ccgcgtggat ggtgaagtag
    11461 tccacgccct gctcggcctg ctcgacgagg gtgtcgcgga agatctccca ggtaagctcc
    11521 tcggccttgc cgtccacctt ctcgagcgcc tggtagatgg gcacgg
//
LOCUS       DASUEV010000125         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_37942, whole genome shotgun sequence.
ACCESSION   DASUEV010000125 DASUEV010000000
VERSION     DASUEV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37942"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="ugpE"
                     /locus_tag="VFV55_04115"
     CDS             <1..889
                     /gene="ugpE"
                     /locus_tag="VFV55_04115"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEX4943513.1"
                     /translation="SSATSSAGSSTDMMVENRPVRDFLAHAVLVVGVLLVAFPLWVTF
                     VASTLTFDLIVTVPMPLWPGDHFLANYTQVLTHGSEKGSSAPVSTMLVNSLIMALSIA
                     IGKISISILSAFAIVYFRFPGRSFFFWSVFVTLMLPVEVRIIPTFKVAADLGILDSHV
                     GLAMPLIASATATFLFRQFFMTIPDELAEAARIDGAGPIRFFIDVVLPLSKTHMAALF
                     VILFIYGWNQYLWPLLITASESMYTVVIGIKRMIAGGDASNEWHLIMATAMLALMPPA
                     LVVVLMQKWFVKGLVETEK"
     gene            986..2053
                     /locus_tag="VFV55_04120"
     CDS             986..2053
                     /locus_tag="VFV55_04120"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEX4943514.1"
                     /translation="MAEISLHGVTKSFGKTEVIHRTDVKIDDGEFVVMVGPSGCGKST
                     VLRMIAGLETTTDGEIRIAGKRVNEVEPKDRNIAMVFQNYALYPHMSVYDNMAYGLKI
                     RKLAKDDIERRVQRAAGILELGPYLKRKPRELSGGQRQRVAMGRAIVREPAAFLFDEP
                     LSNLDAKLRVQMRLEIQKLHRELRTTSVYVTHDQVEAMTLADRMIVINAGVIEQIGAP
                     LEVYDNPASLFVAGFIGSPSMNFLPGKRAGDGVDVGEGLVIPMPAGVKERAGEQVTVG
                     VRPEHFVPVNGSGPVIRFGVDTVEALGADSLLHGVFNAQALVVRIEGHAMPKAGETLM
                     LSPMPGKTFFFDTATGKRLAA"
     gene            2090..2842
                     /locus_tag="VFV55_04125"
     CDS             2090..2842
                     /locus_tag="VFV55_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4943515.1"
                     /translation="MLRRLEELTLNSSAPPGQLLYDGWLLRFSPGKAKRARSINAVYP
                     SRLPLAEKLAHCERVYGERGLPAIYRISAHTDPPQLDAMLAGSGYEPFSPTEVRTADI
                     DPGSLVLDPQREASLASPRLEEWFDMVGDLRGTPIAHRSAHLARLAALPLEMRPAALL
                     EEGVPMATGLAIVEDGHVGLFDVVTREGERRRGYARSIVAALLRWGWERGARRSYLQV
                     DEGNAPAIALYERFGFALAYRYWYRGRPGETK"
     gene            2839..3324
                     /locus_tag="VFV55_04130"
     CDS             2839..3324
                     /locus_tag="VFV55_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX4943516.1"
                     /translation="MSDIDEVSRRLGEACARTHRVLVTAESCTGGGVGEAITRAPGSS
                     AWFDRGFVTYSNESKVDLLGVKQETIGAHGAVSEAVAREMATGALHQSDAHLAVAVTG
                     IAGPEGGTAAKPVGLVWFAWASLDGEVRTKFEVFGGDRAAIRQQAVLEALTGLEALAT
                     G"
BASE COUNT          558 a         1132 c         1164 g          578 t
ORIGIN      
        1 cagttccgct acatcgagcg cagggtccag tactgacatg atggtcgaga acaggccggt
       61 ccgggatttc ctcgcgcacg ccgtgctcgt cgtgggcgtg ctgctggtcg ctttcccgct
      121 gtgggtgacg ttcgtcgcct cgacgctcac tttcgacctg atcgtgacgg tcccgatgcc
      181 gctgtggccg ggggaccatt tcctcgccaa ctacacgcag gtgctcacgc acgggtcgga
      241 gaagggctcc tccgcccccg tctcgacgat gctcgtgaac agcctgatca tggcgctgtc
      301 catcgccatc gggaagatct cgatctcgat cctgtcggcg ttcgcgatcg tgtacttccg
      361 ctttcccggc cggtccttct tcttctggag cgtcttcgtc accctgatgc tgccggtgga
      421 ggtgcgcatc atccccacct tcaaggtcgc cgccgacctc ggcatcctcg actcgcacgt
      481 ggggctcgcg atgccgctca tcgcgtcggc cacggcgacg ttcctgttcc gccagttctt
      541 catgacgatt cccgacgagc tggcggaagc cgcgcgcatc gacggtgcgg gccccatccg
      601 gttcttcatc gacgtggtgc tgccgctgtc gaagacgcac atggccgcgc tcttcgtcat
      661 cctcttcatc tacgggtgga accagtacct gtggcccctt ctcatcacgg cctcggaatc
      721 gatgtacacc gtggtgatcg gcatcaagcg catgatcgcg ggcggggatg cctccaacga
      781 gtggcacctc atcatggcca cggccatgct tgccctcatg cccccggcgc tcgtggtcgt
      841 gctcatgcag aaatggttcg tgaagggcct ggtcgaaaca gaaaaatagt ggtgcagtcg
      901 actgttgaaa cagcgggaac ggtgttggag cggaccgatg gccgctcaac gccggcgggc
      961 atcgaaatca actgagcgaa gacagatggc ggaaatcagc ctgcacggcg tgacaaagag
     1021 cttcggcaag accgaggtca tccatcgaac cgacgtgaag atagacgacg gcgagttcgt
     1081 cgtgatggtc ggcccgtcgg gctgcggcaa gtccaccgtc ctgcgcatga tcgcggggct
     1141 ggagacgacc accgacggcg agatccgcat cgcgggcaag cgcgtgaacg aggtggagcc
     1201 caaggaccgc aatatcgcga tggtgttcca gaactacgcg ctctacccgc acatgagcgt
     1261 gtacgacaac atggcctacg gcctgaagat caggaagctc gccaaggacg acatcgagcg
     1321 gcgcgtgcag cgggccgccg ggatcctcga gctcggcccg tacctgaagc gaaagccccg
     1381 cgagctctcc ggtggccagc ggcagcgcgt ggcgatgggg cgcgcgatcg tgcgcgagcc
     1441 ggcggcgttc ctcttcgacg agccgctgtc gaacctggac gcgaagctgc gcgtgcagat
     1501 gcgcctggag atccagaagc tgcaccggga actgcgcacc acgagcgtgt acgtgaccca
     1561 cgaccaggtc gaggcgatga cgctcgcgga ccggatgatc gtcatcaacg ccggcgtgat
     1621 cgagcagatc ggggcgccgc tcgaggtcta cgacaatccg gcctcgctct tcgtcgcggg
     1681 cttcatcggc tcgccgtcga tgaacttcct gccgggcaag cgtgcgggcg acggcgtgga
     1741 cgtgggcgag gggctcgtca ttcccatgcc ggccggcgtg aaggagcgcg ccggcgagca
     1801 ggtgaccgtc ggcgtccgcc ccgagcattt cgtcccggtg aacgggtccg gcccggtcat
     1861 caggttcggc gtcgacacgg tggaggcgct gggcgcggat tcgcttctgc acggcgtctt
     1921 caacgcgcag gccctggtcg tgcgcatcga agggcacgcc atgccgaagg cgggcgagac
     1981 gctcatgctt tcgccgatgc ccggcaagac cttcttcttc gacaccgcca ccggcaagcg
     2041 cctcgcggcc tgatgggacg ccgcgccgcg ccgcgcaaag gctagcccct tgctccggcg
     2101 actcgaggag ctcacgctca attcgtccgc gccgcccggg cagctcctct acgacgggtg
     2161 gctcctgcgc ttctcgccgg gcaaggcgaa gcgggcgcgc tccatcaacg cggtctaccc
     2221 ttcgcggttg cccctcgcgg aaaagctggc ccattgcgag cgcgtctacg gcgagcgcgg
     2281 gctgcccgcc atctaccgga tctcggcgca caccgatccg ccgcaactcg acgcgatgct
     2341 tgcagggtcc ggctacgaac cgttcagccc gaccgaggtg cggactgccg acatcgaccc
     2401 ggggtcgctt gtcctggatc cgcaaaggga agcgtccctg gcgagcccgc gcctcgagga
     2461 atggttcgac atggtgggcg acctgcgcgg cacgccgatc gcgcatcgca gcgcgcacct
     2521 tgcacgcctg gctgccttgc cgctcgaaat gcgtcccgcg gccctgctcg aggaaggcgt
     2581 gcccatggct accgggctcg ccatcgtcga ggacggccac gtgggtctct tcgacgtggt
     2641 cacgcgcgaa ggcgagcgcc ggcgcgggta cgcaaggtcg atcgtggccg cgctgctgcg
     2701 ctggggatgg gagcgcggcg cgcgccggtc ctacctccag gtggacgagg gcaacgcgcc
     2761 ggccattgcg ctctacgaac ggttcggatt cgcgctagcc tatcgttact ggtaccgcgg
     2821 ccgcccggga gagacgaaat gagcgacatc gacgaagttt cgcgacgcct cggcgaggcc
     2881 tgcgcgcgca cgcaccgggt cctggtgact gccgaatcgt gcaccggcgg cggggtcggc
     2941 gaggcgatca cgcgcgcgcc gggcagttcc gcctggttcg accgcggatt cgtcacctac
     3001 tcgaacgagt ccaaggtcga cctgctgggc gtgaagcagg agacgatcgg tgcccatggc
     3061 gccgtctccg aagcggtggc ccgcgaaatg gcgacgggcg ccctgcacca gagcgatgcg
     3121 cacctggccg tcgccgtcac cggcatcgcc ggccccgagg gcggcaccgc cgccaagccc
     3181 gtcggcctcg tctggttcgc gtgggcgtcc ctcgacgggg aggtccggac gaagttcgag
     3241 gtcttcggtg gtgaccgtgc ggccatccgg cagcaggcgg tgctcgaggc cctgacaggg
     3301 ttggaggcgc tcgccaccgg ctaggggtga aaaggtgaaa aggggtcagg ttactttgca
     3361 caaaaaatag gcaaaacgga gaaggggtca ggttactttt ctgtcgtatt gccgtcgcta
     3421 gacggcgggc tg
//
LOCUS       DASUEV010000126        12129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_839552, whole genome shotgun sequence.
ACCESSION   DASUEV010000126 DASUEV010000000
VERSION     DASUEV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839552"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VFV55_04135"
     CDS             <1..1252
                     /locus_tag="VFV55_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4943517.1"
                     /translation="GSFMARLATLHEAAMAADPSLAAPAARAEQALRAFLAARAEERD
                     AVQKFGFITAGGRVELLTQSISRSVQASLEDKERWRVYLFYYAAALLLGLGYLAARVF
                     AAQAALSEANESLEKRVAARTKELSEALVRLKESEAQLVQSEKMSSLGQMVAGVAHEI
                     NTPLAYVKNSVATVRHRLPELKDAVNQAERLMGLLQSEPASQADLQETVAAVNARLAQ
                     LRDHQVLGDLESLSNDGLHGIEEISELVANLRNFSRLDRSKVASFNVNEGIAATLLIA
                     RPQLRNVSVERHFGEIPSITCSPSQVNQVLLNLVTNAVQALDKSPKTITITTRREGAD
                     AIAFEVADNGKGIAPEALPKIFDPFYTTKEVGKGTGLGLSIAYKIVTEHGGRIGVKSQ
                     PGQGTVFTVVLPLQPPAGLAAGGS"
     gene            1257..3161
                     /locus_tag="VFV55_04140"
     CDS             1257..3161
                     /locus_tag="VFV55_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4943518.1"
                     /translation="MQIPERIGKYVVQSILGKGATGPVYRGFDPGIKRPVALKAITKR
                     LLDPADLDYVINRFRHEAQAVGRLTHPRIAAIYDFIETDEIACIVMELVNGRSLAHHL
                     TEVTRYGFADTWEVVRQVLDGLAYSHGQGVIHRDLKPGNILVNDDGRIKITDFGVARI
                     DTSTLTVMGDIVGTPHYMAPEQFAGQPSTPRTDLYQVGVIVYELLTGARPFTGNNVEI
                     LRRVLNERPADPSTLNPQISRQLDAVVQKALAKDPAERFGSAREFADALRGALEDSLG
                     APLAVTPANGFGAEPGATSLMDKARLIAGARDAGAIGDPAVASAPPSTFATGSDASKA
                     RILFVDDEERILNALRALFRGRYHVFTAGNGALALELARRFGIHVVVSDQRMPNLTGV
                     ELLRQVKDISPNSVRILLTGYSDLAAIVGSINEGEVFRFVKKPWDNDEMQATIADAVA
                     IAMELAAAPPAPAPPAGGIDASILVVDSSAALAEGLKSVLGGLVPVRLVATPDDALKA
                     LEAEEIALIVADLSVGQDGLVTLFKLLKARHPEILSILVTESSDSELVIELINQAHIF
                     RFLNKPVGVRQLRSHVDAALGKYRAFRQQPGLVHTQAVVESAQARTSPWGAKLFERIR
                     ALPDRLFSRA"
     gene            3250..4035
                     /locus_tag="VFV55_04145"
     CDS             3250..4035
                     /locus_tag="VFV55_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX4943519.1"
                     /translation="MTTLTKDELADLKRAKALLENPGVAAKLSSMLGSPVEKGMKMLP
                     SSWQKSVHKATETALMKALDVAVNSLGERRRIVSQDRFHRMAAAASGAAGGAFGLAAL
                     SWELPLSTTLMLRSIADIAASEGENPRHIDTKLACLTVFALGSTKDKRDNATESGYFA
                     TRAALASAISEASAHLARKGLSKSGAPALVRLVATIAARFGVVVSEKAAAQAVPIIGA
                     AGGALVNTVFIGHYQDMARGHFIVRRLEMIHGAEPVRAAYAKV"
     gene            4038..4331
                     /locus_tag="VFV55_04150"
     CDS             4038..4331
                     /locus_tag="VFV55_04150"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX4943520.1"
                     /translation="MTAYVIAEVDVANPEGYSAYTQIVPATISRYGGRFLVRGGAAEV
                     LEGDWPKLRRVIIEFPSVEAARAWWDSPEYEKPKSMRRANSTGRLILLEGFDG"
     gene            complement(4380..5612)
                     /locus_tag="VFV55_04155"
     CDS             complement(4380..5612)
                     /locus_tag="VFV55_04155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX4943521.1"
                     /translation="MNFDSLGLSAPICQAVAAAGYTDPTAVQARVIPEALAGRDLMVS
                     SQTGTGKTAAFMLPALQMLTEAHQVTGRGPRVLVLVPTRELSMQVTKAAETYGRNMGR
                     IKTVSVVGGMPYPAQNRLLQAGVDILVATPGRLLDQMQSGRIDLSRLQLLVLDEADRM
                     LDMGFKEDLEAIVARMPATRQTLLFSATLDSNIVRLAGALLRNPVRIEIDRQQARHEN
                     IVQRMHFADDMGHKNRLLTHLLRDVDMHQAIVFTATKRSADEVAQDLHSQGFSAAALH
                     GDMNQGARTRTLQALRRGGLRVLVATDVAARGIDVQGISHVINYDLPRQAEDYVHRIG
                     RTGRAGRNGTAISLVNSREHAQVKTIERFTAQPIPVSVIEGLEPKVRAFSRPSGPARR
                     PAGKSWAKPAGRSFRQGR"
     gene            5962..7656
                     /locus_tag="VFV55_04160"
     CDS             5962..7656
                     /locus_tag="VFV55_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HEX4943522.1"
                     /translation="MHGLPSRTVHGDSRRYNQERSPPSHESLLPPDSPGFGALLRHIE
                     ALLLLVGLGLLAAAAQAQQDIAVFKTHAGDFAQGQVGAAFTVTVTNVGGAPTPGVVTV
                     TDDVPAGLTPTAAAGAGWVCNIAGAAVTCSRSDALAPGASYPPITVIVNVAPTAPASV
                     TNTATASVGGDVDATNNSSSDVATILPGPDLTISKSHASSFLQGQVGAAFTVTVSNGG
                     GIPTSGAVTVTDNVPAGLTPTAAAGAGWVCNVAGASVTRVRSDPLAPGASYGPITLTV
                     NVAPDAPPSVTNTAIASGGGDVNAANNTASDVATIVPALSAVTPSAGANGSIAPATPQ
                     SVASGGTFIFTVTPDPGYTASVGGTCGGTLAGTTYTTNPITTPCTVVASFSVIPITTF
                     TGPSPTGTGPITATLTGGGAACSFAASQFIPLTGHPRSPPAGSAPAAIDFPHGLFDFT
                     TTGCTPASTITITASYPQLLPPGTQYWKYGPTPMDPAPHWYVLPAAIAGNTATFTITD
                     GTLGDDDLAANGTIVDQGGPGFPLGANQIPTLSEWAMLLLASLMLIAGMRAPRRRA"
     gene            complement(7697..8299)
                     /locus_tag="VFV55_04165"
     CDS             complement(7697..8299)
                     /locus_tag="VFV55_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4943523.1"
                     /translation="MKTVLNVGAGHRDSGSFFPPAFRTAEWKELRLDIDPANEPDVIG
                     TMLDMSAVADGSVDAVFSSHAIEHLYPNELPVAIKEFLRVLRPGGFAVITCPDLQEAA
                     KMIAEDRLMDVAYDSPAGPVTPFDIVFSHRQFTGRDQPYMAHHSGFTLRVLIGTLRAN
                     GFAAVAGTRRPSVFDLWVVATKQPMADEALRELAVKVLPA"
     gene            complement(8313..9218)
                     /gene="hemF"
                     /locus_tag="VFV55_04170"
     CDS             complement(8313..9218)
                     /gene="hemF"
                     /locus_tag="VFV55_04170"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX4943524.1"
                     /translation="MDIASVRDYLAGLQDRIVGKFTQLDGKPFLRDAWERPEGGGGVS
                     RLVEEGNLLERGGVGLSLVSGAKLPPSASAHRPEIAGRPWEAMGVSLVFHPRNPYVPT
                     VHMNVRFFAAKSPDGDPEKAVWWFGGGMDLTPYYGFEEDCAHFHRVNREALAPFGPQY
                     HPRFKKWCDEYFFNKHRNEPRGIGGIFFDDFNEPGFEKSFAMMRAVGDAFLPAYVPIV
                     ERRRDIPYGERERDFQAYRRGRYVEFNLVWDRGTHFGLQSGGRTESILMSLPPLVKWR
                     YDWKPEPGTPEARLYTDFLVAKEWA"
     gene            complement(9224..10522)
                     /gene="purD"
                     /locus_tag="VFV55_04175"
     CDS             complement(9224..10522)
                     /gene="purD"
                     /locus_tag="VFV55_04175"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX4943525.1"
                     /translation="MKLLVIGSGGREHALAWKLAQSPRVSKVLVAPGNAGTAREEGLV
                     NVDVTAIGDLVKLAKDEKVGLTVVGPEAPLAAGVVDLFRAEGLPIFGPTKAAAQLESS
                     KDFAKRFMVRHGIPTAHYATFTEAKAAHAHVDDVVANAPVGETAAPIVIKADGLAAGK
                     GVVVAMTPGEAHGAIDMMLAGGGMGEAGHRVVIEEFMAGEEASFIVMADGKSALAFAS
                     TQDHKRIFDGDRGPNTGGMGAYSPAPVVTPEVHARAMREVILPTLKGMEKEGNPYTGF
                     LYAGLMIDAAGSPRVVEFNCRMGDPETQPIMMRLKGDFLDLLEHALGGTLDRAEAEWD
                     RRAALGVVIAAAGYPESPRKGDEITGLAHDSDDCHVFHAGTAKVDGKVVTAGGRVLCV
                     TALGDKVKFAADRAYEAIAGIRFEGMQYRKDIGYRAIHRR"
     gene            complement(10519..12072)
                     /gene="purH"
                     /locus_tag="VFV55_04180"
     CDS             complement(10519..12072)
                     /gene="purH"
                     /locus_tag="VFV55_04180"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX4943526.1"
                     /translation="MRIQRALVSVSDKTGIVDFAAALVAQGVELLSTGGTAKLLASAG
                     IPVIEVGDYTGFPEMMDGRVKTLHPKVHGGILARRDLPEHVAAMKEHGIPPIDMVVVN
                     LYPFAQTVAKPGCTMDDAIENIDIGGPAMVRASAKNHAFVAIVTDPADYAGLAAKLAA
                     SGGSLGFADRYALAAKAFSHTAEYDGMISSWLTARDESGAAREFPGRLNLQYSLAQSL
                     RYGENPHQSAAFYVERDPARGTLSRFRQLQGKELSYNNIADADAAWECCRTFDVPACV
                     IVKHANPCGVAIAQSPLEAYRRAFLTDPTSAFGGIIAFNRPVDGPTAEAVSKQFVEVV
                     IAPAYDQAAKKVLAAKQNVRLLSIEAAKDFNRFDMKRVGGGLLVQTPDTFDIAELRVV
                     TRKKPSEAQMNDLVFAFRVARFVKSNAIVFCSNGMTLGVGAGQMSRVDSTRIAAIKAK
                     NAGLSLVGSVAASDAFFPFRDGLDVIAREGARAVIQPGGSVRDEEVIDAANGHGMAMV
                     FTGMRHFRH"
BASE COUNT         1935 a         4245 c         4036 g         1913 t
ORIGIN      
        1 cggttccttc atggcgcgcc tcgccaccct tcacgaagcc gccatggccg ccgacccgtc
       61 gctggcggcg ccggcagccc gtgcggagca ggccttgcgc gccttcctgg cggcccgcgc
      121 cgaggagcgc gacgccgtgc agaaattcgg tttcatcacc gccggcggcc gcgtcgagct
      181 cctgacccag tcgatctcgc gctccgtgca ggcgtccctc gaggacaagg agcgctggcg
      241 cgtctacctg ttctactacg ccgcggcgct gctcctgggc ttgggctacc tcgccgcgcg
      301 cgtcttcgcc gcccaggccg cgctaagcga agccaacgaa agcctggaaa agcgcgtggc
      361 cgcgcgcacg aaggagctgt cggaggcgct cgtgcgcctc aaggagtccg aagcgcagct
      421 cgtgcagagc gagaagatgt cctcgctcgg ccagatggtt gcgggcgtgg cccacgagat
      481 caacacgccg ctcgcctacg tgaagaacag cgtggccacg gtgcgccacc gcctgcccga
      541 gctcaaggac gcggtcaacc aggccgagcg cctgatgggg ctgctgcagt cggaaccggc
      601 cagccaggcg gacctgcagg aaacggtcgc cgcggtcaac gcccgcctcg cgcagctgcg
      661 cgaccaccag gtcctcggcg acctcgagtc gctctcgaat gacgggctgc acggcatcga
      721 ggaaatctcg gagctggtcg ccaacctcag gaacttctcg cgcctggacc gaagcaaggt
      781 tgcgagcttc aacgtgaacg agggcatcgc cgccacgctg ctcatcgccc ggccgcagct
      841 gcgcaatgtc tccgtcgagc ggcacttcgg cgaaatcccc tcgatcacct gttcgccctc
      901 gcaggtgaac caggtgctgc tcaatctcgt gaccaacgcc gtccaggccc tcgacaagtc
      961 gccgaagacg atcaccatca ccacgcgccg ggagggcgcc gacgcgatcg ccttcgaggt
     1021 ggccgacaac ggcaagggca tcgccccgga ggcgctgccg aagatcttcg acccgttcta
     1081 caccaccaag gaggtgggca agggcaccgg tctcgggctc tcgatcgcct acaagatcgt
     1141 caccgagcac ggcgggcgca tcggcgtgaa atcgcagccg gggcagggca ccgtgttcac
     1201 ggtggtcctg ccgctgcagc cgccggccgg gctcgcggcc gggggatcct gagcccgtgc
     1261 agatccccga acgaatcggc aagtacgtcg tccagtcgat cctcggcaag ggggccacgg
     1321 ggcccgtcta tcgcggattc gaccccggca tcaagcggcc ggtggcgctc aaggcgatca
     1381 ccaagcgcct gctcgacccc gccgacctgg actacgtgat caaccggttc cgccacgagg
     1441 cgcaggccgt cgggcgcctc acgcacccgc gcatcgcggc catctatgac ttcatcgaga
     1501 ccgacgagat cgcctgcatc gtgatggaac tggtgaacgg caggtcgctg gcgcaccacc
     1561 tcacggaggt tacgcgctac ggcttcgcgg acacctggga ggtcgtccgc caggtgctcg
     1621 acgggctcgc ctattcgcac ggccagggcg tcatccaccg cgacctgaag ccgggcaaca
     1681 tcctcgtgaa cgacgatggc cgcatcaaga tcaccgactt cggggtcgcg cgcatcgaca
     1741 cctccacgct cacggtgatg ggcgacatcg tgggcactcc gcattacatg gcccccgagc
     1801 agttcgccgg ccagccgtcc acgccgcgca cggacctgta ccaggtcggc gtcatcgtct
     1861 acgagctcct caccggcgcg cgccccttca cgggcaacaa tgtcgagatc ctgcgccgcg
     1921 tgctgaacga gcgtcccgcc gacccctcga cgctcaaccc gcagatctcc cggcagctcg
     1981 acgcggtggt gcagaaggcg ctcgccaagg accccgcgga gcgcttcggc agcgcccggg
     2041 aattcgccga cgcgctcagg ggcgcactcg aggacagcct cggcgcgccg ctcgccgtca
     2101 cccccgccaa cggcttcggc gccgagcctg gcgcgacaag cctcatggac aaggctcgcc
     2161 tcatcgccgg ggcacgggac gcgggcgcga tcggcgatcc ggccgtcgcc tcggcgccac
     2221 cttccacctt cgccacgggc agcgacgcga gcaaggcgcg cattctcttc gtggacgacg
     2281 aggaaaggat cctgaacgcc cttcgagccc ttttccgcgg ccggtaccac gtcttcaccg
     2341 ccgggaacgg ggcgctcgcg ctggaactcg ccaggcgctt cggcatccat gtcgtggtga
     2401 gcgaccagcg catgcccaac ctgacgggcg tggagctgct gcggcaggtg aaggatatct
     2461 cgcccaactc ggtgcgcatt ctcctcaccg gctactcgga cctcgccgcg atcgtgggct
     2521 ccatcaacga gggcgaggtg ttccgcttcg tgaagaagcc ctgggacaac gacgagatgc
     2581 aggcgaccat cgcggacgcg gtggccatcg cgatggaact cgcggccgcc ccgccggcgc
     2641 cggcgccgcc ggccgggggt atcgacgctt cgatcctggt ggtcgattcc tccgccgcgc
     2701 tcgccgaggg gctcaagtcc gtgctgggcg gcctggtgcc cgtccgcctc gtcgcgacgc
     2761 cggacgacgc gttgaaggcg ctcgaggccg aggaaatcgc cctcatcgtc gcagacctgt
     2821 ccgtggggca ggatgggctc gtcacgctct tcaagctgct caaggcgcgc cacccggaga
     2881 tcctgtccat cctcgtcacg gagtcctccg attcggaact ggtgatcgag ctcatcaacc
     2941 aggcgcacat cttccgcttc ctcaacaagc cggtcggcgt gcgccagttg cgcagccacg
     3001 tcgacgcggc cctcgggaag taccgcgcct tcaggcagca gcccggtctg gtgcacactc
     3061 aggcggtggt cgagagcgcg caggcgcgga cttcgccgtg gggtgcgaaa ctcttcgagc
     3121 gcatccgcgc cctccccgac cgcctcttct cccgcgcgta gaacaacggg gtcaggttac
     3181 tttactgtcg acgtatttcg actgggctgt aaagtaacct gaccccgttg ttccggtaac
     3241 atcttcgcca tgacgacgct cacgaaagac gaactcgcgg acctgaagcg cgccaaggcg
     3301 ctgctcgaga accccggcgt cgcggccaag ctctcctcga tgctcggctc gccggtcgag
     3361 aagggcatga agatgctgcc ctcctcgtgg cagaagagcg tgcacaaggc gacggagacc
     3421 gcgctcatga aggcgctcga cgtagcggtg aattcgctcg gagagcggcg caggatcgtg
     3481 tcgcaggaca ggtttcaccg catggcagcg gcagcctcgg gggccgcggg cggcgccttt
     3541 gggctcgcgg cgctgagctg ggaactgccg ctttcgacca cgctcatgct gcgctccatc
     3601 gccgacatcg ccgcgagcga gggcgagaac ccgcgccaca tcgacaccaa gctcgcctgc
     3661 ctcacggtgt tcgcgctcgg ctcgacgaag gacaagcgcg acaacgccac ggagtcggga
     3721 tatttcgcga cgcgtgcggc gctcgcctcg gccatctcgg aagcctccgc tcacctggca
     3781 aggaagggcc tgtcgaaatc cggcgcccct gccctggtgc ggctggtcgc gaccattgcc
     3841 gcgcggttcg gcgtggtggt ttcggagaaa gccgccgcgc aggcggtgcc gatcatcggt
     3901 gccgccgggg gtgcgctggt gaacaccgtc ttcatcgggc actaccagga catggcgcgc
     3961 gggcacttca tcgtgcgccg cctcgaaatg atccatggcg ccgagcccgt gcgcgccgcc
     4021 tacgcgaaag tgtgaccatg accgcctacg tgatcgccga ggtcgacgtc gccaaccccg
     4081 agggctacag cgcctacacg cagatagtcc cggccacgat ctcccgttac ggcgggcgat
     4141 tcctcgtgcg cggcggcgcc gccgaggttc tcgagggcga ctggccgaag ctgcgccgcg
     4201 tgatcatcga gtttccctcg gtggaagcgg cgcgcgcctg gtgggactcg ccggagtacg
     4261 agaaacccaa gtcgatgcgc cgggccaatt cgaccggccg cctcatcctc ctcgaaggct
     4321 tcgacggcta ggcgccgtac cgggtacatg taccgggtac atgtacccgg tacaacgtcc
     4381 tagcggccct gccgaaatga tctgccggcg ggcttggccc acgacttacc cgcgggtcgc
     4441 cgggccgggc cggacggccg cgagaacgcg cgcaccttcg gttccaggcc ctcgatcaca
     4501 ctgaccggga tcggctgcgc cgtgaagcgt tcgatggtct tcacctgggc gtgttcgcgc
     4561 gagttcacca ggctgatggc ggttccgttg cggcccgcgc ggccggtgcg gccgatgcgg
     4621 tgcacgtagt cctcggcctg gcgcggcagg tcgtagttga tgacgtgcga gatgccctgc
     4681 acgtcgatgc cgcgcgcggc aacgtcggtt gcgacgagca cgcgcaatcc gccgcggcga
     4741 agcgcctgca gcgtgcgggt gcgggcgccc tggttcatgt cgccgtgcag cgccgccgcc
     4801 gagaatccct gggaatgcag gtcctgcgcc acctcgtcgg ccgatcgctt ggtcgcggtg
     4861 aagacgatcg cctggtgcat gtcgacatcg cgcagcaggt gggtgagcag gcggttcttg
     4921 tgccccatgt cgtcggcgaa atgcatgcgc tgcacgatgt tctcgtggcg cgcctgctgg
     4981 cggtcgatct cgatgcgcac cgggttgcgc agcagcgcgc cggcgaggcg aacgatgttc
     5041 gagtccagcg tggcggaaaa gagcagcgtc tggcgcgtgg cgggcatgcg cgcgacgatg
     5101 gcttcgagat cttccttgaa ccccatgtcg agcatgcggt ccgcctcgtc cagcaccagc
     5161 agctgcaggc gcgagaggtc gatgcggccc gactgcatct ggtcgagtag acgacccggc
     5221 gtggccacca ggatgtcgac gccggcctgc agcaggcggt tctgcgcggg gtagggcatg
     5281 ccgccgacca cggagacggt ctttatccgg cccatgttgc ggccataggt ttcggcggcc
     5341 ttggtgacct gcatcgagag ctcgcgcgtc ggcacgagca cgagcacgcg cgggccgcgg
     5401 ccggtgacct ggtgtgcctc ggtcagcatc tgcagcgccg gcagcatgaa ggcggcagtc
     5461 ttgccggtgc cggtctgcga ggaaaccatc aggtcgcgcc cggcgagggc ctcgggaatc
     5521 acgcgcgcct gcacggcggt gggatcggtg taacccgccg cggcgacggc ctggcagatg
     5581 ggggcggaga ggcccaggga gtcgaagttc attttcagtt ttcccgcgcg gccattccgg
     5641 tgggcgcgct cgtacggcga ttgcgaaaaa ggctcgccgc ccgtcgtcgg aaacggggga
     5701 actcgacacg ccggaatgaa taaagggcgc gcgaagtcgg caggccatga caccggcacc
     5761 aaccgattcg caatccttcg ggaattaacc gtggggaact gctgcgggaa gagagagctc
     5821 tcttccggga aggtcaggga cgatgaacga ttgcggtgcg aattaccgtt gtgctgcacc
     5881 gcacaaaacg aactatacgc aaaagacgtc tgattatcaa gagatttttc cagaccccgg
     5941 ctcgggccga caaccgccgc catgcatgga ctcccctcgc ggacagtcca tggagactcc
     6001 cgtcgatata atcaggaacg ttcgcctcca tcccatgagt ccctgctgcc ccctgattcg
     6061 ccggggtttg gtgcgctgct tcggcacatc gaagctctcc tcctgctcgt tggcctgggg
     6121 ttgctggcgg ccgccgcgca ggcccagcag gacatcgctg tcttcaagac ccatgcggga
     6181 gacttcgctc agggtcaggt gggtgcggcg ttcacggtga ccgtcacgaa tgtgggcggc
     6241 gccccaacgc ctggcgtcgt gaccgtgacc gacgatgttc cggcggggct tacgcctacc
     6301 gcggcagccg gtgccggatg ggtctgcaac attgccggag cggccgtcac ctgctcgcgc
     6361 agtgatgcgc tggcgccggg cgcgagctat ccgccgatca ccgtgatcgt caatgtggcg
     6421 cccactgcgc ccgccagcgt aaccaacacg gcgaccgcga gcgttggcgg agacgtcgat
     6481 gccaccaaca actcgtcgtc ggacgtcgcg accatcctgc cggggcccga cctcaccatt
     6541 tccaagtccc atgcatccag cttcctgcag ggccaggtgg gagcggcgtt cacggtgacc
     6601 gtctcgaatg ggggcggcat cccgacatcg ggcgccgtga ccgtgaccga caacgtgccg
     6661 gcggggctta cgcctaccgc ggcagccggt gccggatggg tctgcaacgt tgccggagcg
     6721 tccgtcaccc gcgtgcgcag cgatccgctg gcgccgggcg cgagctatgg gccgataacc
     6781 ctgaccgtca acgtggcgcc cgatgcgccc cccagcgtga ccaacacggc gatcgcaagc
     6841 ggtggcggcg acgtcaatgc cgcgaacaac acggcatcgg acgtcgcgac catcgtgcca
     6901 gcgttgtctg ccgtgacgcc cagtgccggc gccaacggct ccatcgcccc ggcgacaccc
     6961 cagtcggtcg cgagcggcgg gacgttcatc ttcaccgtga cgcccgatcc gggctacacg
     7021 gcttcggtag gcggcacctg cggcggcacg ctcgcgggaa ccacctacac cacgaatccg
     7081 atcacgaccc cgtgcaccgt cgtcgcaagc ttttccgtga tccccatcac caccttcacc
     7141 ggcccctctc cgaccggcac ggggcccatc accgcgaccc tcaccggcgg cggggcggcc
     7201 tgttccttcg cggccagcca gttcattccg ctcaccggcc atccgcgcag cccgcccgcg
     7261 ggcagcgcgc cggccgccat cgacttcccg catggcctgt tcgacttcac gaccacggga
     7321 tgcacgccgg cgtcgaccat aacgatcacc gcctcctacc cgcaactgct tccaccgggc
     7381 acgcaatact ggaagtacgg ccccacgccg atggatcccg cgccgcactg gtacgtgctg
     7441 ccggccgcga tcgcgggaaa caccgcgacc ttcacgatca ccgacggtac cctgggcgat
     7501 gacgacctcg ccgccaacgg cacgatcgtc gaccagggcg ggccgggatt ccccctcggg
     7561 gccaaccaga ttccgacgct ctcggagtgg gcgatgctgc tgctcgcctc cctcatgctc
     7621 atcgccggga tgcgggcccc gaggcgcagg gcatagccac gggcgatgcg gcgtcccatc
     7681 aggcccggtc cttcggtcag gccggcagca ccttcacggc cagttcccgc agggcctcgt
     7741 cagccatcgg ctgcttcgtc gccaccaccc acaggtcgaa gacgctgggc ctgcgcgtgc
     7801 ccgcgaccgc ggcgaacccg ttggcgcgca gcgtgccgat cagcaccctc agcgtgaatc
     7861 cgctgtggtg ggccatgtag ggctggtccc ggccggtgaa ttgccggtgc gagaagacga
     7921 tgtcgaaggg ggttaccggt ccggccggcg agtcgtaggc cacgtccatc agccggtcct
     7981 ccgcgatcat cttcgcggcc tcctgcaggt cggggcaggt gatgacggcg aagcccccgg
     8041 gccgcaggac gcgcaggaac tcctttatcg ccaccgggag ctcgttgggg tagaggtgct
     8101 cgatggcgtg gctggagaag acggcatcga cgctcccgtc ggccaccgcc gacatatcga
     8161 gcatggtgcc gatgacgtcc ggttcgttgg cgggatcgat gtcgagccgc agttccttcc
     8221 actccgccgt ccggaaggcc gggggaaaga acgagccgct gtcgcggtgg cccgcgccga
     8281 cgttgaggac cgtcttcacg tcacggggcc tgtcaggccc actccttcgc cacgaggaag
     8341 tcggtataga gcctcgcctc gggcgtgccc ggctcgggct tccagtcgta gcgccatttc
     8401 accagcggcg gcagcgacat gaggatggat tccgtgcgcc cgcccgactg caggccgaag
     8461 tgcgtgccgc ggtcccacac caggttgaat tccacgtagc gaccgcggcg ataggcctgg
     8521 aaatcgcgct cgcgctcgcc ataggggatg tcgcggcggc gctcgacgat gggcacatag
     8581 gcgggcagga acgcgtcacc caccgcacgc atcatggcga agctcttctc gaaacccggc
     8641 tcgttgaagt cgtcgaagaa aattccgccg atgccgcggg gctcgttgcg atgcttgttg
     8701 aagaaatact cgtcgcacca cttcttgaag cgcgggtggt actgtgggcc gaagggagcc
     8761 agcgcctcgc ggttgacgcg gtggaagtgg gcgcagtcct cctcgaagcc gtagtagggc
     8821 gtgaggtcca tgccgccgcc gaaccaccac acggccttct ccgggtcgcc gtccggcgat
     8881 ttcgcggcga agaaacgcac gttcatgtgg acggtgggca cgtacggatt cctcgggtgg
     8941 aagacgagcg agacgcccat cgcctcccac ggccggcccg cgatctcggg acggtgcgcc
     9001 gatgccgacg gcggcagttt cgcccccgac acgagcgaca ggcccacgcc gccgcgctcc
     9061 aggagattgc cttcctcgac gaggcgcgac acgccgccgc cgccctcggg gcgctcccac
     9121 gcatcgcgca ggaaaggctt cccgtcgagc tgcgtgaact tgccgacgat gcgatcctgc
     9181 aggccggcga ggtaatccct gaccgaggcg atgtccatgt cggtcagcgc cggtgaatcg
     9241 cccggtaccc gatgtccttg cggtactgca tgccctcgaa gcggattccc gcgatcgcct
     9301 cgtaggcccg gtcggcggcg aacttcacct tgtcgcccag cgcggtgacg cacagcacgc
     9361 gcccgcccgc ggtgacgacc ttgccatcga ccttcgcggt gcccgcgtgg aacacgtggc
     9421 agtcgtccga gtcgtgcgcg aggccggtga tctcgtcgcc ctttcgcggc gactcgggat
     9481 atccggccgc ggcgatcacc acgcccaggg cggcgcggcg gtcccactcc gcttccgcgc
     9541 gatcgagcgt gccaccgagc gcgtgctcca ggaggtcgag gaagtcgccc ttcagccgca
     9601 tcatgatggg ctgcgtttcg gggtcgccca tgcggcagtt gaattccacc acgcgcgggc
     9661 tgcccgccgc atcgatcatg aggcccgcat agagaaaccc ggtatagggg ttgccttcct
     9721 tctccatgcc cttcagcgtc ggcaggatga cctcgcgcat ggcgcgcgca tgcacctcgg
     9781 gcgtcacgac cggcgcgggc gaataggcgc ccatgccgcc cgtgttgggg ccgcggtcgc
     9841 cgtcgaagat ccgcttgtgg tcctgggtgg aagcgaaggc gagcgcgctc ttcccgtccg
     9901 ccatcacgat gaagctcgcc tcctcgccgg ccatgaactc ctcgatcacc acgcggtgcc
     9961 cggcctcgcc catgccgccg cccgcgagca tcatgtcgat ggccccgtgc gcctcgccgg
    10021 gcgtcatcgc gacgaccacc cccttgccgg cggccaggcc gtcggccttg atgacgatgg
    10081 gcgcggctgt ctccccgacc ggcgcattcg cgaccacgtc atccacatgc gcatgcgccg
    10141 ccttcgcctc cgtgaaggtg gcgtagtgcg cggtcgggat gccgtggcgc accatgaagc
    10201 gcttggcgaa atccttggac gactcgagct gcgccgcggc cttcgtcggc ccgaagatcg
    10261 ggagcccctc ggcgcggaag aggtccacca cgcccgccgc gagcggcgcc tccggcccca
    10321 cgacggtgag gcccaccttc tcgtccttcg cgagcttcac gaggtcgccg atggccgtga
    10381 cgtcgacgtt caccagcccc tcctcgcgcg ccgtgcctgc attgcccggg gcaacgagga
    10441 ccttcgagac acgcggggat tgcgcgagct tccaggccag cgcgtgctcc cggccgccgg
    10501 agccgatgac gaggagcttc aatgccggaa gtgccgcatg ccggtgaaca ccatggccat
    10561 gccatgcccg ttcgccgcgt cgatcacctc ttcgtcgcgc acgctcccgc cgggctggat
    10621 gaccgccctg gcgccctccc tcgcgatcac gtccaggccg tcgcggaagg ggaagaaagc
    10681 gtcggatgcc gccaccgagc ccacgaggga aagtcccgcg ttcttcgcct tgatcgcggc
    10741 gatgcgcgtc gaatcgacgc ggctcatctg gccggcgccc acgcccagcg tcatgccgtt
    10801 ggaacaaaag acgatcgcgt tcgacttcac gaaccgggct acgcggaagg cgaagacgag
    10861 gtcgttcatc tgggcctcgc tgggcttctt cctcgtcacc acgcgaagct ccgcgatatc
    10921 gaaggtgtcg ggcgtctgca cgaggagccc gccgcccacg cgcttcatgt cgaagcggtt
    10981 gaaatccttc gccgcttcga tcgagaggag acgcacgttc tgcttggccg ccagcacctt
    11041 cttcgcggcc tggtcgtagg cgggagcgat caccacctcg acgaactgct tcgagacggc
    11101 ttctgccgtg ggcccgtcca ccggccggtt gaacgcgatg atgccgccga acgccgaggt
    11161 cgggtcggtg aggaaggcgc gacggtaggc ctccagcggc gactgcgcga tggccacgcc
    11221 gcacgggttg gcgtgcttga cgatcacgca cgccggcacg tcgaaagtgc ggcagcattc
    11281 ccaggccgcg tccgcatccg cgatgttgtt gtagctcagt tccttgccct gcagctggcg
    11341 gaagcgcgac agcgtccccc tggccgggtc gcgctcgacg tagaaggccg cggactggtg
    11401 cgggttctcg ccgtagcgaa gcgactgggc aagcgagtac tggaggttga ggcggcccgg
    11461 aaattcacgg gcggcgcccg actcgtccct cgccgtgagc cacgaggaga tcatgccgtc
    11521 gtactcggcg gtgtggctga aagccttggc cgccagcgcg tagcggtcgg caaagccgag
    11581 gctgccgccg ctggccgcga gtttcgccgc gagccccgca tagtccgccg ggtcggtgac
    11641 gatggccacg aacgcgtggt tcttggccga ggcccgcacc attgcaggcc cgccgatgtc
    11701 gatattctcg atcgcgtcgt ccatcgtgca gcccggcttc gccacggtct gcgcgaacgg
    11761 atagaggttc accaccacca tgtcgatggg cgggatgccg tgctccttca tggccgccac
    11821 gtgctcggga aggtcgcggc gcgcgaggat gcccccgtgc accttcgggt gcagcgtctt
    11881 cacgcgcccg tccatcattt cgggaaagcc cgtgtagtcg ccgacctcga tgacgggaat
    11941 ccccgccgag gcgagaagct tcgcggtgcc gccggtggag aggagctcga cgccttgcgc
    12001 tacgagcgcg gccgcgaaat cgacgatgcc ggtcttgtcg gagacgctga ccagcgcccg
    12061 ctggattcgg atctggtcgt tttgcatggt ccgtccaaga acggaagcgc aggaaacgtc
    12121 cggctcagt
//
LOCUS       DASUEV010000127        11232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_147354, whole genome shotgun sequence.
ACCESSION   DASUEV010000127 DASUEV010000000
VERSION     DASUEV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147354"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFV55_04185"
     CDS             complement(<1..393)
                     /locus_tag="VFV55_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943527.1"
                     /translation="MARRRKVEAAAGPAALSRGAVLVTGFEPFGGEDINPSWEICKAL
                     PAAIAHARIDTLRVPTEFRRAIEVVAAAIEQLEPALVILLGQAGGRAAISVERVAINV
                     DDARIADNAGRQPVDEPVARAGPAAYFAT"
     gene            complement(399..1190)
                     /locus_tag="VFV55_04190"
     CDS             complement(399..1190)
                     /locus_tag="VFV55_04190"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX4943528.1"
                     /translation="MGKTFAGKSVVITGSTSGIGLGIARAFAAEGANVMLNGLGEVAA
                     IEATRAGLASEFGVKVLYHGADMMKPAEITDLVRTAEKEFGAVDILVNNAGIQHVSPV
                     DEFPLDKWDAIIAINLSSAFHATRAALPGMKKRGWGRVINMASAHGLVASPYKSAYVA
                     AKHGLVGFTKTVALEVAEQGITVNAICPGYTWTPIVEKQIDDQARVHGIPREKVVSDI
                     MLAPQPTKKFIKIEDVARVALNLADISAEQINGTTISIDGGWTAR"
     gene            complement(1216..1842)
                     /locus_tag="VFV55_04195"
     CDS             complement(1216..1842)
                     /locus_tag="VFV55_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4943529.1"
                     /translation="MTLKIYGIAASRAIRALWMAEELGLAYEHVPIHFRDDPDGPLGK
                     NNAGFRRANPMGRVPAIDDDGFTLSESMAINLYLARKHGKGLWPATVAGEGLAFQWSF
                     FAVTEIDPLIVEWASHAIVLPEDKRDPVRAAAALGKLAQPLAVVDAALARSPYLAGDA
                     FTVADLNLAAAMFRARRMDHPTRPNLARWLADCFARPAARRAWAMRGE"
     gene            complement(1839..2294)
                     /locus_tag="VFV55_04200"
     CDS             complement(1839..2294)
                     /locus_tag="VFV55_04200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4943530.1"
                     /translation="MTEWRFARLDELTAREVHDILQARAAVFVVEQACVFQDMDGADP
                     DSWHLFTRVDGELAAYCRIVPAGIKFAEPSIGRVITTGAMRGTGLGRALMLEALRRSA
                     TLWPGAAIRIGAQRRLETFYHSLGFVTDSEPYDEDGIAHVEMVHTGEGT"
     gene            complement(2291..4714)
                     /gene="ppsA"
                     /locus_tag="VFV55_04205"
     CDS             complement(2291..4714)
                     /gene="ppsA"
                     /locus_tag="VFV55_04205"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX4943531.1"
                     /translation="MARAYVLPLETLRMTDVGSVGGKNASLGELISQLSAAGVRVPGG
                     FATTAQAFNDFLDANGLKARIAQALAKLDTEDVGALAVTGAEIREWIATAPLPAELER
                     QIREAYAKVAGDAASAGQGEDPAAPSFAVRSSATAEDLPDASFAGQQETFLNIRGIDN
                     VLDAVRQVFASLYNDRAIAYRVHKGFDHVEVALSAAVQRMVRSDTGAAGVVFTLDTES
                     GFRDVVFVTASYGLGEMVVQGAVNPDEFYVSKVCLAAGRPAILRRGLGSKAIKMVFTS
                     EAKAHRSVVQRDVIPADRDRFCLGDAEVEELARYAMAIEKHYGRPMDIEWGKDGIDGR
                     IYILQARPETVKSQEKKRDHLTRYRIGRRGEVLASGRAIGSKIGQGRVRLVKDVAEIA
                     RVQDGDVLVTDMTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARELGVPAVVGCENATR
                     VLRDGDLVTVSCSEGDTGFIYAGKLDFEVLDVSLDRMPRIPVKLSMNVGNPQLAFDFC
                     QLPNDGVGLARLEFIISNMIGIHPKACIEYAQLPVDLKAQVARQSRGYESPTRFYVEK
                     IVEGVATIAAAFWPKKVIVRLSDFKSNEYRNLIGGAHYEPQEENPMLGFRGASRYIAQ
                     SFRDCFNLECEAMRRVRDDMGLTNVELMIPFVRTLKEAEQVHAILKDNGLERGRNGLK
                     VVMMCEIPSNAILADEFLKHFDGFSIGSNDMTQLTLALDRDSGLVMESFDERDPAVKR
                     MLSLAIQACRRQGKYVGICGQGPSDYPDLAEWLMEEGIESMSLNPDTIVETWLHLAKA
                     ADKVATGAP"
     gene            4864..5688
                     /locus_tag="VFV55_04210"
     CDS             4864..5688
                     /locus_tag="VFV55_04210"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX4943532.1"
                     /translation="MPHRRRVFFVSDRTGLTVEALGSSLISQFDGIDFVRVTLPFIDS
                     ADKARDVVAQIDAAHRETGIRPLVFSSLVNDAVRAEVNKVDGLVLDVFGNFIVPLEQE
                     LGQKSMHAVGKSHSAGNAKDYNHRIEAINYTLAHDDGVTHRGLEEADLVLVGVSRSGK
                     TPTSLYMAMQFGIKAANYPLIPEDLEANRLPPSLMPLKHKVWGLTIHPDRLHQIRKER
                     RPDSRYAALDNCRFEVAAAEKLMRQADIPFLDSTTKSIEEIATTMLHEAHLVRRVY"
     gene            5728..7632
                     /locus_tag="VFV55_04215"
     CDS             5728..7632
                     /locus_tag="VFV55_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX4943533.1"
                     /translation="MKPVRRVLPALLLTVFAGATVPAANAAQFSNVVVFGDSLSDAGF
                     YRPFLASLGLPAPLVATLGRFTTNPGPIWAELISQHYGATPAASNANGWIFAQGGARV
                     ASDSSSTPPGGAQRPVSTQVTEYLARNGGAADPGALHTIWAGANDFFQNFGAFAVGAI
                     NQTQLQANVLGAATAEIQQVGRLYAAGARYVMVFSLPNIGATPQFASLGPAAAGAATA
                     LSAGYNTTLFTGLASTGMRVIPVDTFALLSEIMANPGAYGFSNTTGIACGAFPPITTP
                     TTVSSQFCYTGNLVASGADNSYVFADGVHPTTGAHRIVADFVKSLIDGPTAYSMLGEV
                     PLRTRAGHIQTLSDGLALGQASPGRFNAFASMAGGNFEVESRGNDASFDSNNRSYSVG
                     ITMRANESIVVGAAIGKSKSQGHFGDDLGGFRTSEDTGSIFFGARAGGFYASGAASIA
                     NIDFTQSSRVIKLGSARRVAESAPSGSNASAFVNAGYDFAVGKLSVGPTVAWTSQNID
                     VNAFDESGAGSANLHIGAQKRRSEVASVGIRASYDLGTITPYAKYTADKERRNDDRLI
                     TASPLTFAAGNSYDLPAFQPADSSWGTVTVGIRGRMSDSIAYGINYAKVTGRSGVNED
                     FVFGTLAIRF"
     gene            7697..8005
                     /locus_tag="VFV55_04220"
     CDS             7697..8005
                     /locus_tag="VFV55_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX4943534.1"
                     /translation="MKRVYNAANLPDAYIVAGYLASRGIRVRILNVNAAGAIGELPAD
                     AAAPQLWVEDPRDEARAREAIDAFQALASGPSRRCAACGEENPPAFEVCWSCGRGLEA
                     "
     gene            complement(8011..10224)
                     /locus_tag="VFV55_04225"
     CDS             complement(8011..10224)
                     /locus_tag="VFV55_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4943535.1"
                     /translation="MKPARLCLAFACIAGAVAAPAESLAQAGAPVRPKVGLVLGGGGA
                     RGGAHLGVLEVLEEMRIPFDCVAGTSIGALVGGAYVAGVSPAEMRETIRRTDWNTLFD
                     DSAGRDAVGLRRKYLDDRFYSGLEFGVTTRGLRYREGAVAGEKFKLFFNQLVRTDPGD
                     RNIEELPLPLTLIATDIGTGERVALREGSLAGAMRASMSVPGVIAPVIREGHMLVDGG
                     LVDNLPIQEVRERCGAEVVIAVNVGSPLLKPEEVTGVVSVVGQVVNLLTEQNVAKSLS
                     LLKPGDIYLKPDLGDVTAADFTRQLDAAEAGRKAALGAAEALRTLSVAPAAYREWRAR
                     VRLAPTQAPVIDEIRVAATRFVNPRDLRESIRQREGQPLDAKALAEDLVLIYSRGDLR
                     NLDYSILRERDKTVLKLTPIEKPWGPDYLRFGVNLSSDFRTDSPYNLRALYRKTWINA
                     QGAEWLAAVQLGSDQLISTEFYQPVDERQRWFVRPFASVGLTKVGLFLDGDRLAEYRL
                     RIARAGLDAGANLGAYGQASLGWLERNLRATLDTGPPILPEVKMDFGGPTAAVAIDTQ
                     DFAFFPTRGYRANVNVFDAQRVSTGLGKYGRAEAKFGGAWSLGDLIVLGSLEGGKTLH
                     GTLPLGDTFSLGGQRRLSAFAPDQILGGEYGLGTMQVQWRLTRPLPIFGLSLLAGVSY
                     ETGRMRDPITESNLTGRLDSYGLYLAANTPLGPMYLGYADTSGRSGRFYLFIGTP"
     gene            complement(10238..10963)
                     /locus_tag="VFV55_04230"
     CDS             complement(10238..10963)
                     /locus_tag="VFV55_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX4943536.1"
                     /translation="MQKTPAAEHPQRQYRYYEFVMAAFVTVLICSNLIGPAKIAQVEF
                     PVVGLLTFGAGVLFFPISYVFGDVLTEVYGYARSRRVIWAGFAGLAFASFMAWVVVAL
                     PPAPFWKNQAAYEIAFGQAWRIAAASMFAYFCGEFVNSFTLAKMKILTEGRWLWTRTI
                     GSTIVGEGVDSLLFYPLAFWGTGIIPDEKLPTVMFAQFVAKVGVEAVFTPVTYAVVGW
                     LKRAEHEDYYDRDTDFTPFSVKV"
BASE COUNT         1778 a         3966 c         3705 g         1783 t
ORIGIN      
        1 ggtcgcgaaa taggcggctg gccccgcgcg ggccacgggc tcgtccaccg gctgccggcc
       61 ggcattgtcg gcgatgcgcg cgtcgtccac gttgattgcc acgcgctcca cggaaatcgc
      121 ggcgcgcccg ccggcctgcc ccagcaggat gaccagcgcg ggctccagtt gctcgattgc
      181 ggcggccacg acttcgatgg cgcgacggaa ttcggtcggc acccgcagcg tgtcgatgcg
      241 ggcgtgcgcg atggccgcgg gcagcgcctt gcagatctcc caggacgggt tgatgtcctc
      301 gccgccgaag ggctcgaaac ccgtgacgag gaccgcgccc ctggacaagg cggcaggccc
      361 ggccgcggct tccaccttgc gccggcgagc catcgcggct agcgcgcggt ccagccgccg
      421 tcgatggaaa tcgtggtgcc gttgatctgc tcggccgaga tgtccgcgag gttgagggcg
      481 acgcgcgcca cgtcctcgat cttgatgaac ttcttcgtcg gttgcggggc gagcatgatg
      541 tccgacacca ccttttccct ggggatgccg tgcacgcggg cctggtcgtc gatctgcttc
      601 tcgacgatgg gggtccacgt gtaacccggg cagatggcat tcaccgtgat gccctgctcc
      661 gcgacctcca gcgccacggt cttcgtgaac cccaccaggc cgtgcttggc cgccacgtag
      721 gcgctcttgt acggcgaggc caccaggcca tgggcggacg ccatgttgat cacgcgcccc
      781 cagccgcgct tcttcatgcc cggcagcgcc gcgcgcgtcg cgtggaaggc gctggacagg
      841 ttgatggcga tgatggcgtc ccacttgtcg agcgggaact catccaccgg cgagacgtgc
      901 tggataccgg cgttgttcac caggatgtcg accgcgccga actccttttc cgccgtgcgc
      961 acgaggtcag tgatctcggc gggcttcatc atgtcggcac cgtggtagag gaccttcacg
     1021 ccgaactcgg acgcgaggcc ggcgcgcgtc gcctcgatgg cggccacctc gcccaggccg
     1081 ttgagcatca cgttcgcccc ctcggcggcg aacgcccggg cgatgcccag gccgatgccg
     1141 ctcgtcgacc ccgtgatcac caccgacttt cccgcgaatg tcttgcccat gttcatctcc
     1201 tcctggtgcg ccggctcact cgccgcgcat cgcccacgcc cgccgggcgg cgggtcgcgc
     1261 gaagcagtct gccagccagc gcgccaggtt cgggcgcgtc gggtggtcca tgcgccgcgc
     1321 gcgaaacatc gccgcggcga gattcaggtc cgccaccgtg aaggcgtcgc ccgcgaggta
     1381 cggcgagcgc gcgagcgcgg catcgacgac ggcaagaggc tgtgcaagct tcccgagcgc
     1441 cgccgccgcg cggaccgggt cgcgcttgtc ctcgggcagc acgatggcgt gcgaggccca
     1501 ctcgacgatc aaggggtcga tttcggtgac ggcgaagaac gaccactgga aggcgagccc
     1561 ctcccccgcg acggtggcgg gccacagccc cttgccgtgc ttgcgcgcga ggtacaggtt
     1621 gatcgccatc gactccgaaa gcgtgaaccc gtcgtcgtcg atcgccggca cgcggcccat
     1681 cggattggcg cgccggaagc ccgcgttgtt cttgccgaga gggccgtcgg gatcgtcgcg
     1741 gaaatggatg ggcacgtgct cgtaggccag cccgagttcc tccgccatcc acagggcgcg
     1801 gatcgcgcgc gaggcggcga tcccgtagat tttcagcgtc atgtcccttc tcccgtgtgc
     1861 accatttcaa cgtgcgcaat gccgtcctcg tcgtagggct cggagtccgt cacgaacccg
     1921 agcgaatggt agaacgtctc gagccgccgt tgcgcgccga ttcgaatcgc cgcgccgggc
     1981 cataacgtcg ccgacctgcg cagggcttcc agcatcagcg cacgccccag gccggttccg
     2041 cgcatcgcgc ccgtcgtgat cacgcgcccg atcgagggct ccgcgaactt gatcccggcc
     2101 ggaacgatcc ggcaataggc ggcgagctcg ccgtccaccc gcgtgaagag atgccagctg
     2161 tcgggatcgg cgccgtccat gtcctggaag acgcaagcct gctcgaccac gaatacggcc
     2221 gcgcgcgcct gaaggatgtc gtgcacctcg cgcgccgtga gctcgtcgag gcgcgcgaaa
     2281 cgccattcgg tcatggggcg ccggtggcga ccttgtccgc ggccttcgcc aggtggagcc
     2341 aggtctcgac gatggtgtcc gggttgagcg acatcgactc gatgccctcc tccatcagcc
     2401 actccgcgag gtcggggtag tcgctggggc cctgcccgca gatgcccacg tacttgccct
     2461 ggcgacggca cgcctggatg gcgagcgaga gcatgcgctt caccgccggg tcgcgctcgt
     2521 cgaaggactc catcaccagc cccgagtcgc ggtcgagcgc cagggtgagc tgcgtcatgt
     2581 cgttggagcc gatggagaag ccgtcgaagt gcttcaggaa ctcgtcggcg aggatggcgt
     2641 tcgaggggat ctcgcacatc atcaccacct tgagcccgtt cctgccgcgc tccaggccgt
     2701 tgtccttcag gatcgcgtgc acctgctcgg cctccttcag ggtgcgcacg aacgggatca
     2761 tgagctcgac gttggtgagc cccatgtcgt cgcggaccct gcgcatcgcc tcgcattcca
     2821 ggttgaagca gtcgcggaag gattgcgcga tgtagcgcga ggccccgcgg aagcccagca
     2881 tcgggttctc ctcctgcggc tcgtagtgcg cgccgccgat gaggttgcgg tactcgttcg
     2941 acttgaagtc cgagaggcgc acgatgacct tcttgggcca gaacgccgcg gcgatggtgg
     3001 ccacgccttc cacgatcttc tcgacgtaga agcgcgtcgg gctttcgtag ccgcgcgact
     3061 ggcgcgccac ctgggccttg aggtcgacgg gcagctgcgc gtactcgatg caggccttgg
     3121 gatggatgcc gatcatgttg gaaatgatga attccagccg cgccaggccc acgccgtcgt
     3181 tgggaagctg gcagaaatcg aaggcgagct gcggattgcc cacgttcatc gacagcttca
     3241 ccggaatcct gggcatccgg tcgagggaga cgtccagcac ctcgaagtcg agcttgcccg
     3301 cgtagatgaa gccggtgtcg ccttcggagc aggatacggt gaccaaatcg ccatcccgca
     3361 gcacgcgggt ggcgttctcg cagcccacca ccgccggcac gcccagctcg cgggcgatga
     3421 tcgcggcgtg gcaggtgcgc ccgccccggt tggtgacgat ggcggccgcc cgcttcatca
     3481 ccggctccca gttgggatcg gtcatgtcgg tcacgagcac gtcgccgtcc tgcacgcggg
     3541 cgatttccgc cacgtccttg accaggcgca cgcggccctg cccgatcttc gagccgatgg
     3601 cgcggcccga ggcgagcacc tcgccgcgcc tgccgattcg gtatcgggtg aggtggtccc
     3661 gcttcttctc ctggctcttc accgtctccg gacgcgcctg caggatgtag atgcggccgt
     3721 cgattccgtc cttgccccac tcgatgtcca tcggccggcc gtagtgcttc tcgatcgcca
     3781 tggcatagcg cgcgagttcc tccacctcgg cgtcgccgag gcagaagcgg tcgcggtccg
     3841 cgggaatgac gtcgcgctgg accacggacc tgtgcgcctt cgcctcgctg gtgaagacca
     3901 tcttgatcgc cttgctgccg aggccgcggc gcaggatcgc gggcctgccc gccgcgaggc
     3961 agaccttgga gacatagaac tcgtcggggt tcaccgcgcc ctgcaccacc atctcgccca
     4021 ggccgtagct cgcggtgacg aagacgacat cgcggaagcc cgactccgtg tcgagcgtga
     4081 agacgacgcc cgcggcgccg gtgtcggagc gcaccatgcg ctgcacggcg gcggacagcg
     4141 ccacctccac gtggtcgaag cccttgtgca cgcgataggc gatggcgcga tcgttgtaga
     4201 gcgaggcgaa gacctgccgt accgcatcga gcacgttgtc gatgccgcga atgttgagga
     4261 aggtctcctg ctggccggcg aaggaggcgt cgggcaggtc ctcggccgtg gccgaggagc
     4321 gcacggcgaa gctcggtgcc gcgggatcct caccctggcc ggcggatgcc gcatcgcccg
     4381 cgaccttcgc ataggcctcg cggatctggc gctcgagctc ggccgggagc ggtgccgtcg
     4441 cgatccactc gcggatctcc gcgcccgtca ccgccagcgc gcccacgtcc tcggtgtcga
     4501 gcttcgcgag ggcctgcgcg atcctcgcct tcaggccgtt ggcgtcgagg aagtcgttga
     4561 aggcctgcgc ggtggtggcg aagcccccgg gaacccgcac gccggccgcg gagagctggc
     4621 tgatgagttc gcccagggag gcgttcttgc cgccgaccga ccccacgtcg gtcatgcgca
     4681 gcgtttcgag gggtaatacg taggctctgg ccatgctctc tcgcggggtt cggatgacgg
     4741 gcagttcgga atttgcaatt atatcggacg cttgcggtcg atgctgcgct gcatcaaact
     4801 gccgccacga ccttcgatca tcccgcgccc gcgcgcgaac cccctccatc ccggaaccat
     4861 tccatgcccc accgccgccg cgtattcttc gtctccgacc gcaccggcct caccgtcgag
     4921 gccctcggct cctccctgat ctcccagttc gacggcatcg acttcgtccg cgtgacgttg
     4981 cccttcatcg attccgcgga caaggcccgc gacgtcgtcg cccagatcga cgcggcgcac
     5041 cgggagaccg gcatccgccc cctggtcttc agctcgctcg tgaacgacgc cgtgcgcgcc
     5101 gaagtcaaca aggtggacgg cctcgtgctg gacgtcttcg ggaacttcat cgttccgctc
     5161 gagcaggaac tggggcagaa gtcgatgcac gccgtgggca agagccacag cgccggcaac
     5221 gccaaggact acaaccaccg catcgaggcg atcaactaca ccctcgccca tgacgacggc
     5281 gtcacgcacc gcgggctgga ggaagccgac ctggtgctgg tcggggtctc gaggagcggc
     5341 aagacgccca cgtccctcta catggccatg caattcggca tcaaggcggc caactacccg
     5401 ctgatccccg aggacctcga ggccaaccgc ctgcccccct cgctcatgcc gctcaagcac
     5461 aaggtctggg gtctcaccat ccacccggac cgcctgcacc agatccgcaa ggagcggcgc
     5521 cccgactcgc gctacgcggc gctggacaac tgccgcttcg aagtggccgc cgcggagaag
     5581 ctcatgcgcc aggccgacat ccccttcctc gattcgacga cgaagtccat cgaggagatc
     5641 gccaccacca tgcttcacga ggcacatctc gtgcgtcggg tatattgacg accataacaa
     5701 accaccccaa tccggaggag taaccgaatg aagcccgtcc gtcgcgtcct gcccgccctg
     5761 ctgctcaccg tgttcgccgg cgccaccgtg cccgccgcga acgccgccca gttctccaac
     5821 gtcgtggttt tcggtgacag cctctccgac gcgggcttct accggccgtt cctcgcgagc
     5881 ctcggcctgc ccgccccctt ggtggccacc ctgggccgct tcacgaccaa cccgggtccg
     5941 atctgggcgg aattgatctc acagcattac ggcgccacgc cggcggcctc gaacgcgaac
     6001 ggctggatct tcgcgcaggg cggcgcgcgc gtcgcatcgg actcgtccag cacgcccccg
     6061 ggcggggcgc agcggccggt gagcacccag gtcaccgagt acctcgcccg gaacggcggc
     6121 gcagccgacc ccggcgcgct gcacacgatc tgggccggcg ccaacgattt cttccagaat
     6181 ttcggcgcgt tcgccgtggg cgccatcaac cagacgcagc ttcaggcgaa cgtgctcggc
     6241 gcggccaccg ccgaaatcca gcaggtcggg cgcctctacg cggccggcgc gcgctatgtg
     6301 atggtcttct cgctacccaa catcggcgca acgccgcagt tcgcctcgct cgggcccgcg
     6361 gcggccggcg cggccacggc gctttccgcc ggctacaaca cgacgctctt caccggcctc
     6421 gcctcgacgg gcatgcgcgt cattccggtc gacaccttcg cgctgctctc cgagatcatg
     6481 gcaaacccgg gtgcctacgg cttttccaac acgaccggca tcgcctgcgg cgcgttcccg
     6541 ccgatcacca cgcccaccac cgtgtcgtcg cagttctgct ataccggcaa tctcgtcgcc
     6601 tccggtgccg acaacagcta cgtcttcgcc gacggcgtgc acccgaccac cggcgcgcac
     6661 cgcatcgtcg ccgacttcgt gaagtcgctg atcgacggac cgacggccta ctcgatgctg
     6721 ggcgaagtgc cgctgcgcac gcgggccggc cacatccaga ccctctcgga cggcctcgcg
     6781 ctgggccagg cgtccccggg ccggttcaac gccttcgcgt cgatggcggg cggcaacttc
     6841 gaggtggaat cccggggcaa cgacgcaagc ttcgattcca acaaccgctc ctactcggtg
     6901 ggcatcacga tgcgcgccaa cgagtccatc gtcgtcggcg ccgcgatcgg caagagcaag
     6961 tcccagggcc acttcggcga tgacctgggg ggcttccgca ccagcgagga cacggggtcg
     7021 atcttcttcg gcgcccgggc gggcggcttc tacgccagcg gcgcggcctc gatcgccaac
     7081 atcgacttca cgcaatcgag ccgcgtcatc aagctcggct ccgccaggcg cgtagccgaa
     7141 tcggcgccgt cgggctccaa cgcgtcggca ttcgtgaacg ccggctacga cttcgccgtc
     7201 ggcaagctct ccgtcggccc gaccgtggcg tggacctcgc agaacatcga cgtgaacgcg
     7261 ttcgacgagt cgggcgccgg gtcggcgaac ctgcacatcg gcgcacagaa gcgccgctcg
     7321 gaagtggcga gcgtcggcat ccgcgcctcc tacgacctgg gcaccatcac gccctacgcg
     7381 aagtacaccg ccgacaagga gcgcaggaac gacgaccgcc tcatcaccgc ctcgccgctg
     7441 accttcgcgg ccggcaactc ctacgacctg cccgccttcc agccggccga ttcgagctgg
     7501 ggcacggtca cggtgggcat tcgcggcagg atgagcgact ccatcgccta cgggatcaac
     7561 tatgcgaagg tcacgggccg cagcggcgtg aacgaggatt tcgtcttcgg cacgctcgcc
     7621 atccgcttct gacctcccgc ccgcgaacgg catcgcgaac gggccgcccg cgggcggccc
     7681 gtttcgcttc ctccccgtga aacgcgtcta caacgccgcc aatcttcccg acgcctacat
     7741 cgtggccggg tatctcgctt cgcgcgggat ccgtgtccgc atcctcaatg tcaacgccgc
     7801 gggagccatc ggcgagcttc ctgccgacgc ggcggcgccc cagttgtggg tggaggaccc
     7861 gcgcgacgag gcccgggcga gggaggcgat cgacgccttc caggcgctgg cctccggccc
     7921 gagccggcgg tgcgccgcct gcggcgagga aaatcccccg gccttcgagg tgtgctggtc
     7981 ctgcgggcgc ggcctcgagg cctgacgcgc tcagggcgtc ccgatgaaga ggtagaagcg
     8041 ccccgaacgg ccgctcgtgt ccgcatagcc caggtacatc ggcccgagcg gcgtgttggc
     8101 ggccaggtag agtccgtagg agtcaagccg gccggtgagg ttggattcgg tgatcggatc
     8161 gcgcatgcga cccgtctcgt acgagacccc cgcgagcagc gacaggccga aaatcggcaa
     8221 cggccgcgtg aggcgccact gcacctgcat cgttcccaac ccgtactcgc cgcccaggat
     8281 ctggtccggc gcaaaggccg acagccgcct ttgtccgccg agcgagaagg tgtcgcccag
     8341 ggggagcgtc ccgtggagcg tcttgccccc ctcgaggctg cccagcacga tgaggtcccc
     8401 cagcgaccac gcgccgccga acttcgcctc ggcgcggccg tacttgccga ggcccgtcga
     8461 cacgcgctgc gcgtcgaaga cgttcacgtt cgcgcgatag ccacgcgtcg ggaagaacgc
     8521 gaaatcctgg gtgtcgatcg cgaccgcggc ggtagggccg ccgaaatcca tcttcacttc
     8581 gggcaggatc ggggggcccg tgtcgagggt cgcacgcagg ttgcgctcga gccagcccag
     8641 gctcgcctgc ccgtacgcgc cgaggttggc gcccgcatcc agccccgcgc gcgcgatgcg
     8701 cagccggtat tcggcaaggc ggtcgccgtc gaggaacagc ccgaccttcg tgaggcccac
     8761 ggaggcgaag ggacgcacga accagcgctg gcgttcgtcg accggctggt agaactcggt
     8821 cgagatcagc tggtcgctgc cgagctgcac cgcggcgagc cactccgccc cctgggcgtt
     8881 gatccaggtc ttgcgataca gcgcccgcag gttgtagggg gaatcggtcc ggaaatccga
     8941 agagaggttg accccgaagc gcaggtagtc gggtccccac ggcttctcga tcggcgtgag
     9001 cttgagcacg gtcttgtcgc gctcgcgcag gatggagtaa tcgagattgc gcaggtcgcc
     9061 gcggctgtag atcagcacca ggtcctcggc gagcgccttg gcgtcgagcg gctgcccttc
     9121 gcgctggcgg atgctttcgc gcaggtcgcg cggattgacg aagcgggtgg cggccacgcg
     9181 gatctcgtcg atcaccgggg cctgggtcgg cgcgaggcgc acgcgcgcgc gccattcgcg
     9241 gtaggccgcc ggcgcgaccg acagggtgcg cagcgcctcc gcagcgccaa gggccgcctt
     9301 gcgcccggcc tccgccgcat cgagctggcg cgtgaaatcg gccgcggtga catcgccgag
     9361 gtccggcttc aggtagatgt cgcccggctt caggagggag agcgacttgg cgacgttctg
     9421 ctcggtgaga aggttcacca cctgcccgac cacgctcacc acgcccgtca cctcctcggg
     9481 cttgagcagc ggcgagccca cgttgaccgc gatcaccacc tcggcgccgc aacgctcgcg
     9541 cacttcctgg atcggcaggt tgtccacgag gccgccgtcg acgagcatgt gtccttcgcg
     9601 gatcaccggc gcgatgacgc ccggcacgga catgctcgcg cgcatcgcgc ccgccaggct
     9661 gccctcgcgc aacgcaacgc gctccccggt gccaatgtcg gtggcgatga gggtgagcgg
     9721 cagcggcagt tcctcgatgt tgcggtcccc cgggtcggtg cgcacgagct ggttgaagaa
     9781 gagcttgaac ttctcgccgg ccacggcccc ctcgcggtag cgcaggcccc tggtcgtcac
     9841 gccgaactcg aggccggaat agaaccggtc gtcgagatac ttgcggcgca ggcccacggc
     9901 atcgcgcccg gcgctgtcgt cgaagagcgt gttccagtcg gtcctgcgga ttgtctccct
     9961 catctccgcc ggcgaaaccc ccgcgacgta tgcccctccc acgagtgcgc cgatgctcgt
    10021 cccggcaacg caatcgaacg gtatgcgcat ttcctcgagc acctcgagca ccccgaggtg
    10081 cgccccgccc cgcgcgcccc cgccgcccag gaccaatccg accttgggac gcactggggc
    10141 gcccgcctgg gccagggatt cggccggggc ggcgacggcc cctgcgatgc aggcgaaggc
    10201 gaggcacaat cgggcgggtt tcaccggggg ctcctcctta caccttcacg gagaacggcg
    10261 tgaagtccgt atcgcggtca tagtagtcct cgtgctcggc gcgcttgagc catcccacga
    10321 cggcgtaggt gaccggcgtg aagaccgctt ccacgcccac cttcgcgacg aactgggcga
    10381 acatcaccgt ggggagcttc tcgtcgggga tgatccccgt gccccagaac gccagcggat
    10441 agaaaagcag cgagtccacg ccttcgccca cgatcgtcga gccgatggtg cgcgtccaca
    10501 gccagcgccc ctcggtgagg atcttcatct tggcgagcgt gaacgagttc acgaactcgc
    10561 cgcagaagta ggcgaacatc gaggcggccg cgatgcgcca ggcctgcccg aaggcgatct
    10621 cgtaggcggc ctggttcttc cagaagggtg ccggcggcag cgccaccacc acccaggcca
    10681 tgaaggaggc gaacgcgagg cccgcgaacc cggcccagat gacgcgccgc gagcgcgcat
    10741 agccgtagac ctcggtgagg acgtcgccga agacgtagga gatggggaag aaaagcactc
    10801 cggcgccgaa ggtgaggagc ccgaccacgg ggaactccac ctgggcgatc ttggccgggc
    10861 cgatcaggtt cgagcagatg agcacggtca cgaaggccgc catcacgaac tcgtagtacc
    10921 ggtactggcg ctgcgggtgc tcggctgcgg gagttttttg catgaggtgg gctccgactc
    10981 atccggggtt gggggatcgc gcgacgaatc ccggaactcg tgcgcgatgg gtaatgggcg
    11041 gcggggagtg acgctaccca aaagctagga cacggatgaa cacggatgaa cacggataaa
    11101 ttctgataat gaccgggatc gaggaaatgt cggagtgccg cagacgattc ccttcggtcg
    11161 atcaattcgc tgacgcctcc tccagcccga tcattatcag aatttatccg tgttcatccg
    11221 tgttcatccg tg
//
LOCUS       DASUEV010000128         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_253529, whole genome shotgun sequence.
ACCESSION   DASUEV010000128 DASUEV010000000
VERSION     DASUEV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253529"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFV55_04235"
     CDS             complement(<1..748)
                     /locus_tag="VFV55_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943537.1"
                     /translation="MLKRTAFLLLAIVLATTAFGQGRKRIDKAADLPRFSYKIEGKLE
                     DLVRNEEAFRRFAAEVRRDDQSVLDGYDIADKSAQRGLLGVLAQIDFLEGRYAEAGKR
                     AAQMRALEEKPADKLISGMQLLSMVAAQAKTGNLASDAYRGEVGRIIAAELAKLPYPV
                     IANDIKGMKSTAEIIGEALVMGNVRDRLQPVVDKAGGVLGSDFAPAIVGARYALVARL
                     PLKQTLIETYSAYLAANKVEKPDIWAARAVE"
     gene            889..2235
                     /gene="miaB"
                     /locus_tag="VFV55_04240"
     CDS             889..2235
                     /gene="miaB"
                     /locus_tag="VFV55_04240"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX4943538.1"
                     /translation="MTATARKVFIRTFGCQMNEYDSDKMADVLAASGGFEKTDHIDEA
                     DLILFNTCSVREKAQEKVFADLGRLKPLKQARPGLLIGVGGCVASQEGEEIIRRAPFV
                     DVVFGPQTLHRLPELLAGRRSTGRAQVDISFPEIEKFDHLPPARVDGASAFVSIMEGC
                     SKYCSFCVVPYTRGEEVSRPFDDVLAEVRDLAARGVKEVTLLGQNVNAFAGEMADGEI
                     ADFALLLSHVARVEGIERLRYTTSHPLEFSQRLIDAYGTIPRLVSQVHLPVQSGSDRI
                     LAAMKRNYTAMEYKSIIRRLRAVRPEISVSSDFIVGFPGETEADFAQTMKLVDDVRFD
                     GSFSFVYSARPGTPAAGLADDTPQAVKLERLQRLQMRIQELAQEYSRAMVGTRQRVLV
                     EGPSRKDPSELAGRTDNNRVVNFPGDARLVGQFVDVDITQALPHSLRGAVAPAATA"
     gene            2320..5028
                     /locus_tag="VFV55_04245"
     CDS             2320..5028
                     /locus_tag="VFV55_04245"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX4943539.1"
                     /translation="MNKLYIVSVAAFAALAIASTASAQAPAPSDPAAAAPAAPAAPAA
                     PPAPAATPAGPLKPFKDVLKDAKAIPGYFTLHQKDEKVWIELKPEQFDKPFFFSMNIP
                     RSIGERGLYGGQMVGPWFPMPGGSQVAVWKKIGNQVQLIAKNTEFFASAGTPQARFVE
                     ESFSDSLIASATAAAQPHPDTKAVVIEGNALLFADLPGYSTMIETAFRMPFALDAKNS
                     SFSRVTNTENMTSLQVNAHFAVPKISAPPVTPPPVPAPPPPKATPDPRSFFVGFQYNF
                     MPLPATPMAARPADERIGHFNVSRVDYTDDVSPKLRSHYVNRWRLEKKDPAAAVSEPK
                     EPITYWLDKNIPEKYRKSVADGILEWNKAFEKAGFRNAVVVKQQTEKDDFDTMDSRHA
                     SVRWFTGADVGFAIGPSHVDPRSGEILDADIGMSDVFGRGARRLVSEDLARTFGDEAP
                     VPETFSYAQRSARGFLACNYLNESAREMNFASDLLESRGLDMSSPEADALAQAYVKDV
                     VMHEVGHTLGFQHNFRSSSIYTLKQIQDPEFTKKNGVTGSVMDYTPFNISVKGEPQGE
                     YAMSTLGPYDYWAVEYAYKEIAPASEKEELARIASRSTEPALAFGNDEDAGYGNIAIG
                     MDPEVNRFDLGADPLDYYRHRLKLSRELWDRIQTLQLTPGESYERLTRSFTSGFAQLA
                     RVAPLAAKFVGGVRHLRDHAGSGRPLYEPTPVARQREALAMITGDFLKVDSFRFRPEF
                     VSRIGIDQFDRPRNPDISIAAAVLKVQSAVLDQLMADVVAARILDSQDKVEDRKKAIQ
                     LSEVYDTLQGAIWAELRTGQDITGMRRNLQRDHLRRVAGALVKPAATTPADARALQRE
                     NAIVLQKQIRAALAKPGMSKEARAHLGESLNTLTEALKAPLARAGV"
     gene            5164..>5757
                     /locus_tag="VFV55_04250"
     CDS             5164..>5757
                     /locus_tag="VFV55_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX4943540.1"
                     /translation="MLCGALDENLRQIETALDVAIARRSERFSISGNPEQVGLAARAL
                     RKFYDLAARDLSVEEIQLGLVEISRTPAGGGPESPALRLPRKSELTARTPRQAQYLQQ
                     MKTHDITFGVGPAGTGKTFLAVAAAVDSFERDAVKRIVLTRPAVEAGEKLGFLPGDLA
                     QKVDPYLRPLYDALYDLMGYDKVGRLFERGAIEVAPLA"
BASE COUNT          955 a         2013 c         1884 g          905 t
ORIGIN      
        1 gctcgaccgc gcgtgccgcc cagatgtcgg gcttctcgac cttgttcgcc gccaggtagg
       61 ccgagtaggt ctcgatcagg gtctgcttga ggggcagccg cgccacgagc gcgtagcggg
      121 cgcccacgat ggcgggcgcg aagtcggagc cgagcacgcc gcccgccttg tcgacgacgg
      181 gctgcaggcg gtcgcggacg ttgcccatca ccagcgcttc gccgatgatc tcggccgtgg
      241 atttcatgcc cttgatgtcg ttcgcgatca ccgggtacgg cagcttcgcg agttcggccg
      301 cgatgatcct ccccacctcg ccgcgatagg cgtcggaagc gaggtttccc gtcttcgcct
      361 gcgcggcgac catcgagagc agttgcatgc ccgagatgag cttgtcggcg ggcttctcct
      421 cgagcgcacg catctgggcg gcgcgcttgc cggcctccgc atagcgcccc tcgaggaagt
      481 cgatctgcgc cagcacgccc agcaggccgc gttgcgcgga cttgtcggcg atgtcgtacc
      541 cgtcgaggac ggattggtcg tcgcgccgca cctctgccgc aaagcggcgg aaggcttcct
      601 cgttgcgcac gaggtcctcg agcttgccct cgatcttgta ggagaagcgt ggcaggtcgg
      661 ccgccttgtc gatgcgcttc ctgccctggc cgaaggcggt ggtcgccagc acgatcgcca
      721 gcagcaagaa tgcggttcgc ttgagcattc gcaggttcct ccagggggtg cggccattcg
      781 ggcaggcccg aaggccggaa gcggccgatt atagcgatgc ggtaatatct cgctcctcca
      841 tcgccttttg cctgcccgga ttcaacctcc gcgacccagg ccgacgccat gaccgctacc
      901 gccaggaagg tcttcatccg tactttcggg tgccagatga acgagtacga ctcggacaag
      961 atggccgatg tgctggccgc gtccggcgga ttcgagaaga ccgaccatat cgacgaggcc
     1021 gacctcatcc tcttcaacac ctgctcggtg cgcgaaaagg cgcaggagaa ggtgttcgcg
     1081 gacctcggcc ggctcaagcc cctcaagcag gcgaggcccg gactcctgat cggcgtgggc
     1141 gggtgcgtgg cgagccagga gggcgaggag atcattcgcc gggcgccctt cgtggacgtg
     1201 gtgttcggcc cgcagacgct gcaccgcctc cccgagctgc tcgccggccg gcgctcgacg
     1261 ggccgggcgc aggtcgacat ctcgttcccc gagatcgaga agttcgacca cctcccgccc
     1321 gcgcgcgtgg acggggcgag cgccttcgtg tcgatcatgg agggctgcag caagtactgc
     1381 agcttctgcg tcgtgcccta cacgcgcggc gaggaagttt cgcgtccctt cgacgacgtg
     1441 ctcgcggagg tgcgcgacct cgccgcccgg ggcgtgaagg aagtcacgct cctcggccag
     1501 aacgtgaacg ccttcgcggg cgagatggcc gatggcgaga ttgccgactt cgccctgctc
     1561 ctgtcgcacg tggccagggt ggaggggatc gagcgcctgc gctacacgac ctcgcatccg
     1621 ctcgaattct cgcagcgcct catcgacgcc tatggaacga tccccaggct cgtctcgcag
     1681 gtgcacctgc cggtgcagtc gggctcggac cggatcctcg ccgccatgaa gcgcaactac
     1741 acggcgatgg agtacaagtc gatcatccgc cggctgcgtg cggtccggcc ggagatctcc
     1801 gtctccagcg acttcatcgt gggctttccc ggcgagaccg aggcggactt cgcgcaaacg
     1861 atgaagctcg tggacgacgt gcgcttcgac ggctcgttca gcttcgtcta cagcgcgcgt
     1921 cccggcaccc cggcggcggg tctcgccgac gacacgccgc aggcggtgaa gctcgaacgc
     1981 ctgcagcggt tgcagatgcg gatccaggaa ctggcgcagg agtactcgcg cgccatggtt
     2041 ggcacccgcc agcgcgtgct ggtcgagggg ccgtcgagga aggacccgtc ggagctcgcc
     2101 ggccggacgg acaacaatcg cgtcgtgaac tttcccggcg acgcgcggct cgtggggcaa
     2161 ttcgtcgacg tagacatcac gcaggcgctg ccccacagcc tgcgcggcgc cgtggctccg
     2221 gccgcgacgg catgaaccca tcgtgacttt ccgtgacaaa cccggtcccg cggcgtgcta
     2281 ttttgcgtcg cttcaaggac ccgtaacgcg aggagcctca tgaacaagct gtacatcgtg
     2341 tccgtcgccg ccttcgccgc actcgccatc gcctcgaccg cctcggccca ggccccggct
     2401 ccgagcgatc ccgccgccgc tgcgcctgcc gcacccgctg cgcctgccgc gccccctgcg
     2461 cccgccgcca cgcccgccgg ccctctgaaa ccgttcaagg atgtgctcaa ggatgcgaag
     2521 gccatcccgg gctatttcac gctgcaccag aaggacgaga aggtgtggat cgagctcaag
     2581 cccgagcagt tcgacaagcc cttcttcttc tcgatgaaca tcccgcgttc catcggcgag
     2641 cgcggacttt atggcggcca gatggtgggc ccgtggttcc cgatgccggg cggaagccag
     2701 gtggccgtat ggaagaagat cggcaaccag gtgcagctca tcgcgaagaa caccgagttc
     2761 ttcgcgagcg cgggcacgcc ccaggctcgt ttcgtcgagg agtccttctc cgacagcctc
     2821 atcgcgtcgg ccaccgcggc ggcgcagccc catccggaca cgaaagcggt cgtcatcgaa
     2881 ggcaacgcgc tgctgttcgc cgacctgccg gggtactcga ccatgatcga gacggcattc
     2941 cgcatgcctt tcgcgctgga tgcgaagaat tccagcttca gccgggtcac caacaccgag
     3001 aacatgacga gcctgcaggt gaacgcgcat ttcgccgtgc ccaagatctc ggcgccgccc
     3061 gtgactccgc cgccggtgcc tgccccgccg ccgcccaagg ccacgccgga tccgcgcagc
     3121 ttcttcgtgg gcttccagta caacttcatg ccgctgcccg ctacgccgat ggccgcgcgc
     3181 ccggccgacg agcgcatcgg ccacttcaac gtctcgcgcg tcgactacac ggatgacgtg
     3241 tcgcccaagc tgcgttcgca ttacgtgaac aggtggcggc tggagaagaa ggatcccgcc
     3301 gccgcggtgt cggagccgaa ggagcccatc acctactggc tcgacaagaa catcccggag
     3361 aagtaccgca agtcggtcgc cgacggcatc ctcgagtgga acaaggcctt cgagaaggcg
     3421 ggcttccgca acgccgtcgt cgtgaagcag cagacggaga aggacgattt cgacaccatg
     3481 gactcgcgcc acgcatcggt gcgctggttc accggcgccg acgtgggctt cgccatcggg
     3541 ccttcgcacg tcgacccgcg ttccggggag atcctggacg ccgacatcgg catgtcggac
     3601 gtcttcgggc gcggcgctcg ccgcctggtt tccgaggacc tcgcccgcac cttcggcgac
     3661 gaggcgccgg tgccggaaac cttctcctac gcgcaacgtt ccgcacgcgg attcctcgcc
     3721 tgcaattacc tgaacgagtc cgcccgcgag atgaatttcg cgtccgacct gctcgaatcg
     3781 cgtggtctcg acatgtcgag ccccgaagcc gatgcgctgg cccaggccta cgtgaaggac
     3841 gtggtcatgc acgaggtcgg gcatacgctc ggcttccagc acaacttccg ctcctccagc
     3901 atctacacgc tgaagcagat ccaggacccg gagttcacga agaagaacgg ggtcaccggc
     3961 tcggtgatgg actacacgcc gttcaacatc tcggtgaagg gcgagccgca gggcgagtac
     4021 gccatgagca cgctcgggcc ctacgactac tgggccgtgg agtacgccta caaggagatc
     4081 gcaccggcct ccgagaagga ggagctcgcg aggatcgcgt cgcgctccac ggagccggcg
     4141 ctcgccttcg gcaacgacga ggacgccggc tacggcaaca tcgcgatcgg catggacccc
     4201 gaggtgaacc gcttcgacct gggcgccgac ccgctcgact actaccgcca ccggctcaag
     4261 ctctcgcgcg agttgtggga tcgcatccag accctgcaac tcacccccgg cgagagctac
     4321 gaacggctca cgcgcagctt caccagcgga ttcgcgcaac tcgcacgggt cgcgccgctc
     4381 gcggccaagt tcgtcggcgg cgtgcgtcac ttgcgcgatc atgccggatc gggccggccg
     4441 ctctacgagc ccacgccggt tgccaggcag cgcgaggcgc tggccatgat caccggcgac
     4501 ttcctcaagg tcgacagctt ccgcttcagg ccggagttcg tgagccgcat cggcatcgac
     4561 cagttcgatc gcccgcgcaa tcccgacatc tcgatcgcgg cggccgtact caaggtgcaa
     4621 agcgccgtgc tcgaccagct catggccgac gtcgtcgccg cccggatcct cgattcgcag
     4681 gacaaggtgg aagaccggaa gaaggcgatc cagctttccg aggtctatga cacgctgcag
     4741 ggcgccatct gggcggagct caggacgggc caggacatca ccggcatgcg ccgcaacctg
     4801 caacgcgacc acctgcgccg cgtggccggc gcgctcgtga agcccgctgc caccacgccg
     4861 gccgacgcgc gggcgctgca gcgcgagaac gcgatcgtgc tgcagaagca gatccgcgcc
     4921 gcgctggcca agcccggcat gtccaaggag gcgagggcgc acctgggcga aagcctcaac
     4981 acgctcaccg aggcgctcaa ggcgcccctc gcacgggcgg gcgtctaggg aaccgcgccc
     5041 cggctcccgg gccccgctac aataggcctg ccggtcgttc cggcaggcct attgtccttt
     5101 cgccacgtga agaaccagac ccaggaggtg gcgttcgcgc cggtcgacaa cgcgcggctc
     5161 gcgatgctgt gcggtgcgct ggacgagaac ctccggcaga tcgagaccgc gctggacgtc
     5221 gccatcgcgc gccgctccga gcgcttcagc atttcgggca atcccgagca ggtggggctg
     5281 gccgcgcggg cgctgcgcaa gttctacgac ctcgcggcgc gcgacctctc ggtggaggaa
     5341 atccagctcg gcctggtgga gatctcgcgc acgcctgccg gcggcggacc cgaatcgccg
     5401 gcgctgcgat tgccgcgcaa gagcgaactg accgcgcgca cgccgcggca ggcccagtac
     5461 ctgcagcaga tgaagaccca tgacatcacg ttcggcgtcg ggcccgctgg cacgggcaag
     5521 accttcctcg cggtggccgc cgccgtggat tccttcgagc gcgacgccgt gaagcgcatc
     5581 gtcctcacgc gcccggcggt ggaggccggt gagaaactgg ggttcctgcc cggcgatctc
     5641 gcgcagaagg tcgacccgta cctgcgcccg ctctacgacg cgctctacga cctcatgggc
     5701 tacgacaagg tgggcaggtt gttcgagcgc ggcgccatcg aggtcgcgcc gctggcc
//
LOCUS       DASUEV010000129        15295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_840133, whole genome shotgun sequence.
ACCESSION   DASUEV010000129 DASUEV010000000
VERSION     DASUEV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840133"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..932
                     /locus_tag="VFV55_04255"
     CDS             438..932
                     /locus_tag="VFV55_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX4943541.1"
                     /translation="MSPGTAVSPNQPSPSQPLAAVPRTGVAAPAPASAAPVRAASDSA
                     PRVEEGAGSKLIVGPNIKLRGVEIDDCDTLVVEGRVEATMVSRSIQIAETGSFKGSAD
                     IEVAEIRGAFDGELTVRGRLVVHATGRISGKVRYGKLLVQEGGELTGDIGTLAERSGI
                     VKTA"
     gene            complement(929..2296)
                     /gene="purB"
                     /locus_tag="VFV55_04260"
     CDS             complement(929..2296)
                     /gene="purB"
                     /locus_tag="VFV55_04260"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX4943542.1"
                     /translation="MSHALTALSPLDGRYAAKTRALQEHFSEFALIRLRVRVEIAWLK
                     ALASDPAFVLLPAFSTATVEELDRVAAGFSVADAERVKAIEATTNHDVKAVEYWLRDR
                     FRGNAEVSAAGEFIHFACTSEDINNLAHALMVRGGLEGVLLPAIDAIAARLDELAQAH
                     ADVAMLSRTHGQPATPTTLGKEMANVAWRLKRARLATARVEMLGKLNGASGNFNAHVV
                     AAPAMDWPGFARRFVTGLGIAYNPLTIQIEPHDALAEAFDALARLNTILVDLDRDVWG
                     YISLGYFRQKVKPGEVGSSTMPHKVNPIDFENSEGNLGLANALLRHLAEKLPVSRWQR
                     DLTDSTVLRNMGVAFGYCLLGYEACLRGLGKLEANPARIAADLDGCWDVLAEPVQTVM
                     RAHRIAGSYEKLKDLTRGKGGITQEELHRFVRSLPLPDDAKARLLALTPATYVGLAAT
                     LARAR"
     gene            2397..3002
                     /locus_tag="VFV55_04265"
     CDS             2397..3002
                     /locus_tag="VFV55_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4943543.1"
                     /translation="MKLVASKTSPYARKVRVLLAEKQLAFEFVEESAWNPGTTVPRFN
                     PLNKIPVLVTDDGTFLYDSGVIAEYLDPLGAPSFIPPSGLERACVRRDEALGDGIADA
                     GIAIFLERKREAARQDPAWISRQRSKVDAGIGALARELGGKAYLRGDTLSLGDVACAC
                     ALLWLEFRLPEIAWRSQHAALGRWIERLEARPSFVLTRPPA"
     gene            complement(3041..4108)
                     /gene="mnmA"
                     /locus_tag="VFV55_04270"
     CDS             complement(3041..4108)
                     /gene="mnmA"
                     /locus_tag="VFV55_04270"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX4943544.1"
                     /translation="MKIVVGLSGGVDSAVAALLLKRQGHEVIGLFMKNWEDDDDDEHC
                     SSREDLVDAVSVADRIGIEVEAVNFAAEYRERVFASFLAEYRAGRTPNPDVLCNAEIK
                     FRAFLDHAVAMGAERIATGHYARVEEHGGRFVLLKGKDPAKDQSYFLHRLTQAQLSRA
                     VFPLGHLRKTEVRAIAREAGLAVHAKKDSTGICFIGERPFREFLSRYLPREPGPIVTP
                     SGERLGEHHGLMYHTLGQRQGLGIGGRRGGDGAPWYVAAKDLASNTLVVVQGHDDARL
                     FSSRLRAQDASWIEGSPPRDDGKLAAKTRYRQADSPCSLATGEAGAFTLEFGQPQWAV
                     TPGQSAVIYRGEVCLGGGVIA"
     gene            complement(4202..4513)
                     /locus_tag="VFV55_04275"
     CDS             complement(4202..4513)
                     /locus_tag="VFV55_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943545.1"
                     /translation="MKKLSALIAAVFASVSLGAFAQAPAKPAVPATPAVAATPATPGV
                     KPAEPAKPTVAATPAVPSAKATVKTAEAPAAPSKSAKKKVSKKKAVAKKVEDKLDAPK
                     K"
     gene            complement(4635..5183)
                     /locus_tag="VFV55_04280"
     CDS             complement(4635..5183)
                     /locus_tag="VFV55_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX4943546.1"
                     /translation="MPMLILVNKPYGFVCQFSPSGDRPTLAGLVPVPGVYPAGRLDTD
                     SEGLLVLTDDGRLQARIADPRHKLEKAYWVQVEGLPGEDQLEAMRRGVDLGDFVTRPA
                     RVDAIEEPAHLWPRDPPIRVRRNIPTSWLDVRIREGRNRQVRRMTASTGLPTLRLIRY
                     QVGPWTLAGLAPGQWREASPAL"
     gene            5294..6508
                     /locus_tag="VFV55_04285"
     CDS             5294..6508
                     /locus_tag="VFV55_04285"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX4943547.1"
                     /translation="MSKIKVKNPVVEMDGDEMTRIIWKRIREKLILPYLDIDLKYYDL
                     GMEHRDATDDKVTVDSANATKEYGVAVKCATITPDEARVKEFNLKQMWKSPNGTIRNI
                     LDGTIFREPIICRNVPRIVPHWDKPVVVARHGYGDVYKATEMKFDGPGTIKLTFVPKG
                     GGAPVEKEVFQAPGAGITMAMYNLDDSIRGFARACFNYALTRNYPVYLSTKNTILKVY
                     DGRFKNLFQETFDAEFKAKFAAAGLTYEHRLIDDMVASNLKWSGGYLWACKNYDGDVQ
                     SDTIAQGYGSLGLMTSVLTTPDGKTVEAEAAHGTVTRHYRQHQEGKPTSTNPIASIYA
                     WTRGLQYRGRMDNTPDVVNFANTLEKVCVDMVESGKMTKDLALLIGPDQPFLTTEAYM
                     DAVDAELKRRMG"
     gene            complement(6588..6791)
                     /locus_tag="VFV55_04290"
     CDS             complement(6588..6791)
                     /locus_tag="VFV55_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX4943548.1"
                     /translation="MATGTVKWFNDAKGYGFITPDDGSEDLFAHFSAIQMQGFKTLKE
                     GQKVSFDVTQGPKGKQASNIQAA"
     gene            7022..7330
                     /gene="clpS"
                     /locus_tag="VFV55_04295"
     CDS             7022..7330
                     /gene="clpS"
                     /locus_tag="VFV55_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX4943549.1"
                     /translation="MPSKSSGSTLLKPSAAKVEPPPMYKVVMYNDDFTPMEFVVEVLQ
                     MFFALPREQATQVMLKVHTEGRGVCGTYPKDVASTKVEQVIQYSRQHQHPLQCAMEES
                     "
     gene            7327..9606
                     /gene="clpA"
                     /locus_tag="VFV55_04300"
     CDS             7327..9606
                     /gene="clpA"
                     /locus_tag="VFV55_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX4943550.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALCDNPSASEVL
                     KACAAKIDELKKALADFVMQHTPTVAGTGEVDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIYGEKDSHAVYFLHQQGVSRLDVVNFISHGITKNPQSGARDEGESAEEGQ
                     EAPGAGGALESFTQNLNQLAIDGKIDPLIGREAELERVIQILCRRRKNNPLLVGEAGV
                     GKTAIAEGLAKRVIDSQVPEVLAKAQVYALDMGALLAGTKYRGDFEQRLKAVLKQLVE
                     NPNAILFIDEIHTLIGAGSASGGTLDASNLLKPALSSGALKCIGATTYNEFRGIFEKD
                     HALSRRFQKIDVNEPSIQETVEILRGLKSRFESHHGVKYTANALTTAAELSARYINDR
                     HLPDKAIDVIDEAGALQRILPKSRQKKVIGKPEIEEIIAKIARIPARSVSSDDRTQLR
                     NLDRDLRATVFGQDAAIEALSAAIKMARSGLGNPQKPIGSFLFSGPTGVGKTEVARQL
                     AFIMGVELVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKHPYSVLLLDEI
                     EKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVVIVMTTNAGAEGLSKNAIGFATDRK
                     TGDEMAEIKRLFTPEFRNRLDAVISFAALDENVILRVVDKFLMQLEAQLGEKKVEAQF
                     TEGLKKYLAKNGFDPLMGARPMARLIQDTIRRALADELLFGRLAGGGRVTIDVDSDGK
                     VKLDIATATPSGEPVPTAT"
     gene            9735..10082
                     /locus_tag="VFV55_04305"
     CDS             9735..10082
                     /locus_tag="VFV55_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4943551.1"
                     /translation="MIGIARYLGVAAVSLAAGLAVAQQPAPPAFAPSNLSPAGIRSLA
                     ANCAACHGTNGRPASGSTLAGLAGKPRNEMSNSLTQFKEGKKPATVMHQLAKGYSDEE
                     LEALADYFSKQPR"
     gene            10100..11371
                     /locus_tag="VFV55_04310"
     CDS             10100..11371
                     /locus_tag="VFV55_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HEX4943552.1"
                     /translation="MSMQRRDFLKAGMAAAAAAGLAGCAGLPAAGSKARVVVVGGGYG
                     GATAAKYVRLFDPSIEVVLVEPNEAFVSCPISNLVLGGFKTMNDITTPYTGLANNHGV
                     RIVRDMATAIDADKKQVRLAKGETLSFDRVIVSPGIDFIPGAMPALENAEAQARVLHA
                     WKAGPQTVALRRQLEDMPDGGVYVLAIPEAPYRCPPGPYERACQVAAYFKARKPKSKV
                     LVLDANGDVTSKGPLFKKAWAELYKGIIEYRPNSKAVDVDLRAGAVKLELDDVKGNVL
                     NVVPPMKAGKIADAFITANKRWCEVDWITFESKAARGVHLLGDSTLSAPLMPKSGHMA
                     NAHGKACAAAVVALLNGDTPNQSPTLSNTCYSFVSDTEVVHVASIHKYDAAARTFKTV
                     PGSGGVSSARNEMEAGFALNWARNIWADTLT"
     gene            11501..12880
                     /locus_tag="VFV55_04315"
     CDS             11501..12880
                     /locus_tag="VFV55_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX4943553.1"
                     /translation="MNFHDGKPNPGARPAMRPDPMTRKRLLVLFPDEWDRSMAARRDG
                     VHDFLFEGFDLFRFPDNARLFTFDVARFVDRLERRYASAGLDGILTSDEQFGPVVASL
                     LGERLGLPHNPIRAVLTAQHKYYARLAFERSLPECNPRFCLVVRDFRHTRELPLAFPL
                     YVKPVKAAFSVLARRIDSFDALDRHARFSWFEQAIIERLVKPFGDVMRAHAGFDVEPF
                     TMIAEELIDGVQVTVNGFARAGAVTMLGVIDSIMYPGTDQFQRFQYPSALPRELQSGA
                     EEVARRALAAVGFTHGMFNVELRLCTSTGLAKVIEINPRAAGQFYDLFERVDGYNLFD
                     ALVALETGGEPAIRHRQGRDAFAASFVLRDLEGAGLGRWPARGEIGRLRARHPEATIM
                     FYPKRGADLRREMKWLGSYRYGVVNLGAPTLGELFANYGRIHRDIAFHPRGQESSRVD
                     EMLGEAAAD"
     gene            complement(12944..13525)
                     /locus_tag="VFV55_04320"
     CDS             complement(12944..13525)
                     /locus_tag="VFV55_04320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4943554.1"
                     /translation="MDFPEIETARLRLVIARPGLELAFARFYADNFAGHLDRWSPPPP
                     QDGFGEDHWAKRLRVFEKEFREGTTARWAMLSPNAAAPEVIGTCNFTQIVKGPFRACV
                     LGYQVDRRHEGHGLMHEALGATIAYAFGELRLHRIMANYRPENARSGRLLERLGFLRE
                     GYAREYLFIDGAWRDHVLTSRTNPLFDAAWLRE"
     gene            complement(13548..14120)
                     /gene="slmA"
                     /locus_tag="VFV55_04325"
     CDS             complement(13548..14120)
                     /gene="slmA"
                     /locus_tag="VFV55_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX4943555.1"
                     /translation="MAKTGERKAQILQMLASMLEEPKAERVTTALLAARLDVSEAALY
                     RHFASKAQMYEGLIEFIEQTLFTLVNRIQSDEADAMKQVESMLVALLAFAQKNPGMTR
                     VLTGDALAIEDERLQLRVNQLVDRLEASIRQSTRLAVSAGQLPADWDANAYATTLIAY
                     VIGRWHLFAKSGFKRSPTEGWPQHWRVLSA"
     gene            complement(14134..>15295)
                     /locus_tag="VFV55_04330"
     CDS             complement(14134..>15295)
                     /locus_tag="VFV55_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX4943556.1"
                     /translation="RATVTEMGPLLLARILDLNETQAGVLAAVFKIADDDGLALLDLK
                     DLRALLQHAGDNAAQFRTRYGNISTASIGAIQRGLLALESQGGEKFFGEPVLELDDLM
                     QTLDGKGVVNILAADKLLASPRVYATMLLWLLSELFEQLPEVGDPEKPKLVFFFDEAH
                     LLFSDAPREMLDRIEQVVRLVRSKGVGVWFVTQNPLDVPDTVLGQLGNRVQHALRAYT
                     PRDQQAVKAAATTFRSNPKLDVEKAITELGVGEALVSFLDEKGRPFPVERAFVIPPRS
                     RIGPITPDERRKAIESSLVAGQYEKAVDRESAYEKLAARAGERAQGEETGGSVLGGIL
                     GKLGLPGGDAAKGRTRETAIEAAAKSAARAMGSEVGRRIIRGVLGSILGGRR"
BASE COUNT         2612 a         5142 c         5108 g         2433 t
ORIGIN      
        1 gtccttggcg aatgccgtgc cggtggcggc gaggagcgcg atggcggcgg cggcgatgaa
       61 cttcttcatg gtttctcctg ttgggtgggg tcaggctacg tgcgacacga attctagcgg
      121 cttcgcgggc accctgcgca cccggtgcgt gggccggaat tgccgcctgc gagcgacgtc
      181 tccgtgggga gacgggcacg aactgcatca aatcaacccg ttgcgcgccc tggcccgatc
      241 ggctgtcgcg ggatggcgac gatagcttcc tgcgctttcc gcggccgact tgccgaaacc
      301 cgcgtggatc ctcgatccgc gggaaacgtg ccgatgtggc acggatcgtg ggtgaggtcc
      361 gacagttcaa ccgacaagga gcagtcatga gatacgcaga cggattattc ggccggaagg
      421 atgcaccgcg aggcgcgatg tccccgggaa ccgccgtttc cccgaaccag ccttcgccca
      481 gccagcccct ggccgccgtt ccccgtaccg gtgtcgcggc gcctgcgccc gcgtcggctg
      541 cgccggtgcg cgctgcatcc gattccgcgc cgcgcgtcga ggagggagcc ggctcgaagc
      601 tgatcgtcgg ccccaacatc aagctcaggg gcgtggagat cgacgactgc gacacgctgg
      661 tcgtcgaagg ccgtgtcgag gcgacgatgg tgagccgttc gatccagatc gccgagaccg
      721 gctcgttcaa gggctcggca gacatcgagg tcgccgagat ccgcggcgct ttcgacggcg
      781 agcttaccgt tcgcggccgg ctcgtcgtcc acgcgaccgg ccggatcagc ggcaaggtgc
      841 gctacgggaa gctcctggtc caggaggggg gtgaattgac cggcgacatc ggcacgctcg
      901 cggagcgctc cgggatcgtg aagaccgcct agcgcgcgcg cgcgagcgtc gcggcaagcc
      961 cgacgtaggt cgccggtgtg agcgcgagca ggcgcgcctt ggcatcgtcg ggcaggggca
     1021 gcgagcgcac gaagcggtgc agttcctcct gcgtgatgcc gcctttcccc cgcgtgaggt
     1081 ccttcagctt ctcgtaggac cccgcgatgc ggtgggcgcg catcaccgtc tgcacgggct
     1141 cggccagcac gtcccagcat ccgtcgagat cggcggcaat gcgagccgga ttcgcctcga
     1201 gcttgccgag gccgcgcagg cacgcctcat accccagcag gcaatagccg aatgccacgc
     1261 ccatgttgcg cagcaccgtg gaatcggtga ggtcgcgttg ccaccgtgag accggcaact
     1321 tctcggccag gtggcgcagc agcgcattgg cgagcccgag gttgccctcg gaattctcga
     1381 agtcgatcgg gttcaccttg tgcggcatcg tcgaggagcc cacctcgccc ggcttcacct
     1441 tctggcggaa gtacccgagc gaaatgtagc cccacacgtc gcggtccagg tcgacgagga
     1501 tggtgttgag cctcgcgagg gcgtcgaagg cctccgcgag cgcgtcgtgg ggttcgatct
     1561 ggatcgtgag ggggttgtag gcgatgccga gcccggtcac gaaccgccgc gcgaagccgg
     1621 gccagtccat ggcgggcgcg gcgacgacgt gggcattgaa gtttccggaa gcgccgttca
     1681 gcttgccgag catctcgacg cgggccgtgg cgagccgggc gcgtttcagc cgccaggcca
     1741 cgttggccat ttccttgccg agcgtggtcg gcgtggccgg ctgcccatgg gtgcgcgaga
     1801 gcatggccac gtcggcgtgc gcttgcgcca gctcatcgag gcgcgcggcg atcgcgtcga
     1861 tcgcgggcag caggacgcct tccaggccgc cgcgcaccat cagggcatgc gcgaggttgt
     1921 tgatgtcctc ggaggtgcac gcgaaatgga tgaactcccc ggcagccgag acttccgcgt
     1981 tgccgcggaa ccggtcgcgc agccagtact cgacggcctt cacgtcgtgg ttcgtcgtcg
     2041 cctcgatggc cttcacgcgc tccgcgtcgg ccacggagaa cccggcggcg acccggtcga
     2101 gctcctccac cgtagccgtg gaaaaggcgg gaagcaggac gaaggcgggg tcggacgcga
     2161 gcgccttcag ccacgcgatt tcgacacgca cgcgcaggcg gatcagcgcg aactcgctga
     2221 aatgctcctg caacgcgcgg gtcttggcgg cgtagcgccc gtctagcggc gaaagggcgg
     2281 taagggcgtg ggacatggcg gaaccgtggg cggattttat cacgcggccg cgagctgccg
     2341 gccgccggat accccggtgc tatactccgg ccgccgcccg ggattgagga gcccgcatga
     2401 agctagtcgc atcgaagacc agcccttacg cacgcaaggt ccgggtgctg ctcgccgaga
     2461 agcagcttgc cttcgaattc gtcgaggaaa gcgcgtggaa ccccggcacg accgttcccc
     2521 ggttcaaccc gctcaacaag atacccgtgc tggtgacgga cgacggcacc ttcctctacg
     2581 actcgggcgt gatcgcggag tacctcgatc ccctcggcgc tccgtccttc atcccaccct
     2641 ccggcctcga gcgcgcctgc gtcaggcgcg acgaggcgct cggtgacggg atcgcggacg
     2701 cggggatcgc gatcttccta gagcgcaagc gcgaggcggc ccgccaggac cccgcctgga
     2761 tatcccggca gcgcagcaag gtggacgcgg gcatcggggc gctcgcgcgc gagctgggcg
     2821 gcaaggcata cctgcgcggc gacacgctgt cgctcggcga cgtggcctgt gcctgcgcgc
     2881 tgctctggct ggaattccgc ctgccggaga tcgcctggcg ctcgcaacac gccgcgctcg
     2941 gccggtggat cgagaggctc gaggctcgcc cgtcgttcgt gcttacccgg ccgccggcct
     3001 gaacatcgca gtccccggcg gccatctcgt cgaccttgcc tcaggcaatc acgcccccgc
     3061 ccaggcacac ctcaccgcgg tagatcaccg ccgactgccc cggcgtcacg gcccactgcg
     3121 gttggccgaa ctcgagcgtg aatgcgccgg cttcacccgt cgcgagcgag catggcgaat
     3181 ccgcctgccg gtaacgcgtc ttcgcggcga gcttcccgtc gtcccggggt ggcgagcctt
     3241 cgatccagct tgcgtcctgc gcgcgaaggc gtgaagagaa gagtcgcgca tcatcgtgcc
     3301 cctgtacgac gaccagggtg ttcgaggcga ggtccttcgc ggcgacgtac cacggtgcgc
     3361 cgtcgccccc gcggcggccc ccgataccga gtccctggcg ctgccccagc gtgtggtaca
     3421 tgaggccgtg gtgctccccg aggcgctcgc cgctcggggt gacgatggga cccggctcgc
     3481 gcggcaggta gcgcgagagg aattcgcgga aggggcgttc cccgatgaag caaatgcccg
     3541 tggagtcctt cttggcgtgg acggcaagcc cggcctcgcg cgcaatggcc cggacctcgg
     3601 tcttgcgcag gtgtcccagc ggaaagacgg ccctggaaag ctgggcctgg gtcaggcgat
     3661 gaaggaagta cgattggtcc ttcgcgggat ccttcccctt caggagcacg aagcggccgc
     3721 cgtgttcctc gacccgggcg taatgtccgg tggcgatccg ctcggctccc atcgccacgg
     3781 cgtggtccag gaacgccctg aacttgatct cggcattgca cagcacgtcc ggattgggcg
     3841 tgcggccggc acggtattcg gcaaggaagc tcgcgaacac gcgctcccgg tactccgccg
     3901 cgaagttcac ggcctcgacc tcgatgccaa tccggtcggc gacggacacc gcgtcaacga
     3961 ggtcctcgcg gctcgagcag tgctcgtcgt cgtcgtcatc ctcccagttc ttcatgaaca
     4021 ggccgatcac ctcgtggccc tgccgcttga ggagcagcgc ggcgacggcc gaatcgacac
     4081 ctccggagag cccgacgaca atcttcatgg aacgctatct tgcggcaaat gggcgaaccg
     4141 ggcaggccaa acgaaaaagg cagggccgaa gccctgcctt cccggtcccg tgttgacggg
     4201 attacttctt gggcgcgtcc agcttgtcct cgaccttctt ggcgaccgcc ttcttcttgc
     4261 tgaccttctt cttggcggac ttggaaggtg ccgccggggc ctcggccgtc ttcaccgtgg
     4321 ccttcgcgga cggaacggcg ggagttgccg cgacggtggg cttggcgggt tcggcgggct
     4381 tgacgcccgg ggtggccggg gtcgccgcga cggcgggggt agcaggcacg gcgggcttgg
     4441 caggcgcctg ggcaaacgcg cccagcgaaa cggatgcgaa cacggcggcg atcagtgcag
     4501 acagcttctt catttcggta cctttgcgtt aagtggggaa ttttgcttcg actgcgctgg
     4561 aagccagcct cgtcacggcg aataacgcag caggtgcggc cccggttgac cggcgcggga
     4621 acggccgcac ggaatcagag ggcaggtgac gcctcccgcc actggcccgg ggcgaggccg
     4681 gccagggtcc acgggccgac ctggtaccgg atgagccgca acgtgggcag gcccgtgctt
     4741 gcggtcattc gccggacctg ccggttgcgt ccctcccgga tgcgcacgtc gagccaggaa
     4801 gtcggaatgt tccggcgcac ccggatggga ggatcgcggg gccaaaggtg cgcgggctcc
     4861 tcgatcgcat ccacccgggc cgggcgggtc acgaaatccc ccaggtcgac gcccctgcgc
     4921 atcgcctcga gctggtcctc cccgggcaac ccttccacct gaacccagta cgccttctcg
     4981 agcttgtgcc gggggtcggc aatccgcgcc tgcaggcgac cgtcgtcggt gaggaccagg
     5041 agtccctcgc tgtccgtgtc gagccgcccc gcgggataga caccgggaac gggcaccagg
     5101 cccgcgaggg tcgggcgatc ccccgacggg ctgaactggc agacgaaacc gtacggtttg
     5161 ttgaccagga tcagcatggg catggggggg agtatcggaa ggctttgcta taattccacg
     5221 cttttcgcac cgccgtcagc gccggtcgcg atattcgccc gccccccaaa gacgccccac
     5281 aggagtcgtg ctcatgagca agatcaaggt caagaacccg gtcgtcgaga tggatggcga
     5341 cgagatgact cgcatcatct ggaagaggat ccgcgagaag ctgatcctcc cgtacctcga
     5401 catcgacctc aagtactacg acctcgggat ggagcaccgc gatgccaccg acgacaaggt
     5461 cacggtcgac tccgccaatg ccacgaagga gtatggggtg gcggtgaagt gcgcgaccat
     5521 cacgcccgac gaggcgcgcg tcaaggaatt caacctgaag cagatgtgga agagtcccaa
     5581 cggcacgatc cgcaacatcc tcgatggcac cattttccgc gagcccatca tctgcaggaa
     5641 cgttccgcgc atcgtcccgc actgggacaa gcccgtcgtc gtggcccgcc acggctacgg
     5701 ggacgtgtac aaggcgacgg aaatgaagtt cgatggccct ggcacgatca agctcacgtt
     5761 cgtcccgaaa ggcggcggcg caccggtcga gaaggaggtg ttccaggcac ccggcgcggg
     5821 catcacgatg gcgatgtaca acctggacga ctcgatccgc ggattcgccc gcgcgtgctt
     5881 caactacgcg cttacccgga actacccggt ctacctgtcg accaagaaca cgatcctcaa
     5941 ggtctacgac ggtcgcttca agaacctctt ccaggagacc ttcgacgcgg agttcaaggc
     6001 caagtttgcc gccgccggcc tcacctacga acaccggctg atcgacgaca tggtcgcctc
     6061 gaacctcaag tggagcggtg gctacctgtg ggcgtgcaag aattacgacg gcgacgtgca
     6121 gtccgacacc atcgcccagg gctacggctc gctgggcctc atgacctccg tcctcacgac
     6181 gccggacggc aagaccgtcg aggcggaggc ggcccacggc acggtgacgc gccactaccg
     6241 gcagcaccag gagggcaagc ccacgtcgac caaccccatc gcctcgatct acgcctggac
     6301 gcgcggcctg cagtaccgcg gccggatgga caacacgccg gacgtggtga acttcgcgaa
     6361 cacgctcgag aaggtgtgcg tggacatggt ggagtccggc aagatgacga aggacctcgc
     6421 gctgctcatc ggtcccgacc agccgttcct caccaccgag gcgtacatgg acgccgtcga
     6481 tgcggagctc aagcgccgca tgggatgaca ccggacgcgc ggccggaaaa caaaaagccc
     6541 ccggggacgc gagcctcggg ggctttccag ttctggtcag tcggctctta ggcggcctgt
     6601 atgttcgacg cctgcttgcc tttcgggcct tgcgtcacgt cgaaggagac cttctggcct
     6661 tccttgaggg tcttgaaacc ctgcatctgg atggcggaga agtgggcaaa gagatcctcg
     6721 ctgccgtcat ccggcgtgat gaacccgtag ccttttgcat cgttgaacca cttgacggta
     6781 ccggttgcca ttcgcttgca tacctcttgg ggaaacgggt ggaggcccgc acattgttcg
     6841 gatccaggcc cgcacggcga aatggcgatg cgaaatcctt gaagccaaac tccaacgcgc
     6901 cttgtacgcc cgttgcccgg gcaagtcaat agagcgggcg gggtcttgaa aaaccgccgt
     6961 cctttgtcca tattgaacag gaccgaaacg acggatgccc gcaccatccc gcgccaaagg
     7021 catgccaagc aagtccagcg gctcaacttt gctcaagccg agcgcggcga aggtcgagcc
     7081 gccgccgatg tacaaagtcg tcatgtataa cgatgacttc acgcccatgg aattcgtggt
     7141 ggaggtactg cagatgttct tcgcgcttcc ccgggaacaa gcgactcagg tgatgctcaa
     7201 agtgcatacc gaggggcgcg gcgtgtgcgg cacctacccg aaggacgtgg cctccacgaa
     7261 agtggagcag gtaattcaat attcgcgcca gcaccagcat cccctgcagt gcgcgatgga
     7321 ggaatcatga tcgcccagga gttggaagtc agcctgcaca tggccttcgt cgaggcccgc
     7381 cagaagcgcc acgagttcat caccgtggag caccttctcc tcgccctgtg cgacaacccg
     7441 tcggcctcgg aggtgctcaa ggcgtgcgcg gccaagatcg acgagctgaa gaaggcgctg
     7501 gccgacttcg tgatgcagca cacgccgacg gtcgcaggca cgggcgaggt cgacacccag
     7561 cccacgctgg gcttccagcg ggtcatccag cgcgccatcc tgcacgtgca gtcctcgggc
     7621 aagaaggagg tgaccggcgc caacgtgctg gtggccatct acggcgagaa ggattcgcac
     7681 gccgtctact tcctgcacca gcagggcgtc tcgcggctgg acgtggtgaa cttcatctcg
     7741 cacggaatca ccaagaaccc gcagtcggga gcgcgcgatg aaggcgaaag cgccgaggag
     7801 ggccaggaag ccccgggcgc cggcggcgcg ctggagagct tcacgcagaa cctcaaccag
     7861 ctcgcgatcg acggcaagat cgacccgctc atcggccgcg aggccgagct cgagcgcgtg
     7921 atccagatcc tctgccgccg ccgcaagaac aacccgctgc tcgtcgggga ggccggcgtg
     7981 ggcaagaccg ccatcgccga ggggctcgcc aagcgcgtga tcgactccca ggtgcccgag
     8041 gtactcgcca aggcgcaggt ttacgcgctg gacatgggcg cgctcctcgc gggcaccaag
     8101 taccgcggtg atttcgagca gcgcctcaag gcggtcctca agcagctcgt ggagaacccc
     8161 aacgcgatcc tcttcatcga cgagatccac accctcatcg gtgccgggag cgcctccggc
     8221 ggcacgctgg acgcctccaa cctgctcaag ccggcccttt cgtcgggcgc gctcaagtgc
     8281 atcggcgcca cgacgtataa cgagttccgc ggcatcttcg agaaggacca cgcgctttcg
     8341 cgccgcttcc agaagatcga cgtgaacgag ccctccatcc aggagacggt cgagatcctg
     8401 cgcggcctca agtcgcgctt cgagtcgcac cacggggtga agtacacggc caacgcgctc
     8461 accacggccg cggagctttc cgcccgctac atcaacgacc gccatctccc cgacaaggcg
     8521 atcgacgtga tcgacgaggc gggggccctg cagcgcatcc tgcccaagtc gcggcagaag
     8581 aaggtgatcg gcaagcccga gatcgaggaa atcatcgcga agatcgcgcg catcccggcg
     8641 cgcagcgtct cctccgacga ccgtacccag ctgaggaacc tcgaccgcga cctccgggcg
     8701 accgtgttcg gccaggacgc ggccatcgag gcgctgtcgg ccgcgatcaa gatggcacgt
     8761 tccggactgg gcaacccgca gaagccgatc ggctcattcc tgttctcggg ccccaccggc
     8821 gtgggcaaga ccgaggttgc acggcagctg gcgttcatca tgggcgtcga gctcgtgcgc
     8881 ttcgacatgt ccgagtacat ggagcgccac gcggtctcgc gcctcatcgg cgcgcctccg
     8941 ggctacgtcg gattcgacca gggcgggctc ctcacggaag cgatcacgaa gcatccctat
     9001 tcggtgcttc tgctcgacga gatcgagaag gcgcacccgg acatcttcaa catcctgctg
     9061 caggtgatgg accacggcac gctcacggac aacaacggcc gcaaggccga tttccgcaac
     9121 gtcgtgatcg tgatgacgac caacgcgggc gccgagggac tgtcgaagaa cgccatcggc
     9181 ttcgccaccg accgcaagac cggcgacgaa atggccgaga tcaagcgcct cttcacgccg
     9241 gagttccgca accgcctgga cgcggtcatt tcctttgccg cgctcgacga gaacgtcatc
     9301 ctgcgggtcg tggacaagtt cctcatgcag ctcgaggcac agctgggcga gaagaaggtc
     9361 gaggcgcagt tcacggaggg cctcaagaag tacctcgcga agaacgggtt cgacccgctc
     9421 atgggcgcac gccccatggc gaggctcatc caggacacca tccgccgcgc gcttgccgac
     9481 gagctcctct tcgggcggct cgcgggcggg ggacgcgtga ccatcgacgt ggacagcgac
     9541 ggcaaggtga aactcgatat cgccaccgcc acgccttccg gcgagccggt cccgaccgcc
     9601 acctgacctg cgcgcggcgc gcatcgccgc cgatccccat tgggggcata cccccctgac
     9661 catcgggacg ggtttacagg cgccggaccc ccggatactc tcgggaggcg cccaaaaacc
     9721 tgaggagaag cccgatgatc ggaatcgcgc gatacctggg ggtggcagcg gtctcgctgg
     9781 cggccggact tgccgtcgcc cagcaacccg ccccgcccgc gtttgcccca tccaacctct
     9841 cgccggcagg gatacgttcc ctggccgcga actgtgccgc ctgccacggc acgaacggcc
     9901 ggcccgccag cggctcgacg cttgccgggc tggccggaaa gccgcgcaat gaaatgagca
     9961 actccctgac ccagttcaag gagggcaaga aacccgcgac ggtcatgcac cagctcgcca
    10021 agggctattc ggacgaggag ctcgaggccc tcgccgacta cttctcgaag caaccccgct
    10081 aggcgaccgg aggacacaca tgagcatgca acgaagggat ttcctcaagg cgggtatggc
    10141 tgcggcagcg gcggcgggcc tcgcgggctg cgcggggctt ccggcagccg gttccaaggc
    10201 gcgcgtggtg gtcgtcggcg gcggttacgg cggcgcgacg gcagccaagt acgtacggct
    10261 gttcgatccg tccatcgagg tggtgctggt cgagcccaac gaggcattcg tctcgtgccc
    10321 gatctccaac ctcgtgctgg gcggcttcaa gacgatgaac gacatcacga cgccctacac
    10381 cggcctcgcg aacaaccacg gcgtgcgcat cgtgcgcgac atggcgaccg ccatcgacgc
    10441 ggacaagaag caggtgcgcc ttgcgaaggg cgagaccctg tccttcgacc gcgtgatcgt
    10501 gtcgcccggc atcgacttca tccccggcgc gatgccggcc ctcgaaaatg cggaggcgca
    10561 ggcacgcgtg ctgcatgcct ggaaggccgg cccgcagacg gtggcacttc gccgccagct
    10621 cgaggacatg cccgacggcg gcgtgtacgt cctggccatt ccggaggcgc cctaccgctg
    10681 ccccccggga ccctacgagc gcgcctgcca ggtcgcggcc tacttcaagg cgagaaaacc
    10741 gaagtcgaag gtgctggtcc tcgacgccaa tggcgatgtc acctcgaagg ggcccctctt
    10801 caagaaggcg tgggcggaac tctacaaggg aatcatcgaa taccggccca acagcaaggc
    10861 ggtcgacgtg gacctgcgcg cgggtgccgt aaagctcgaa ctggatgacg tgaagggcaa
    10921 cgtgctcaac gtggtgccgc cgatgaaggc ggggaagatc gccgatgcct tcatcaccgc
    10981 gaacaagcgc tggtgcgagg tggactggat cacgttcgag tcgaaggccg ccaggggcgt
    11041 gcatctgctg ggcgactcga cattgagcgc cccgctcatg cccaagtccg ggcacatggc
    11101 caacgcgcac gggaaggcct gcgcggcggc cgtggtggcg ctgctcaacg gcgacacgcc
    11161 gaaccagtcg ccaacgctct cgaacacctg ttacagcttc gtttccgaca ccgaagtcgt
    11221 gcacgtggcc tccatccaca agtacgacgc ggccgcgcgc acgttcaaga cggtcccggg
    11281 atccggtggc gtgtcgtcgg cgaggaacga aatggaagcg ggcttcgcgc tcaactgggc
    11341 acgcaacatc tgggccgata cgctcacctg agatcgttac gatgaagcgc gaacgggccg
    11401 cctcggcggc ccgttcgcat cacgcgctgc gccaggcttc agagcgccgt ggaatacggg
    11461 cattcaatcc gctgttgacg gcgtaagctt ccggtaagag atgaacttcc atgatggcaa
    11521 gccgaatccg ggcgcaaggc cggcgatgcg cccggacccg atgacgcgca agcggctcct
    11581 cgtccttttt cccgacgagt gggaccgctc gatggcggcg cggcgcgacg gggtccacga
    11641 cttcctgttc gagggattcg accttttccg ctttccggac aacgcgcgcc tcttcacatt
    11701 cgatgtcgcg cgattcgtcg accggctgga acgccgctat gccagcgccg ggctggacgg
    11761 aatactcacc tccgacgaac agttcggtcc cgttgtcgcc tccctcctgg gagagcgcct
    11821 gggccttccg cacaacccca taagggcggt gctcactgcc cagcacaagt attacgcgcg
    11881 ccttgcgttc gagagatcgc tccccgagtg caatccccgc ttctgcctgg tcgtccggga
    11941 cttccgccac acccgcgagc ttccgctcgc gttcccgctg tacgtgaagc cggtcaaggc
    12001 ggcgttctcg gtgctcgcgc gccgcatcga ctcgttcgac gcgcttgacc gccacgcgcg
    12061 cttctcgtgg ttcgagcagg cgatcatcga gaggctcgtg aagcctttcg gcgacgtgat
    12121 gcgcgcccat gcgggcttcg acgtcgagcc attcaccatg atcgccgagg aactgatcga
    12181 cggggtgcag gtcaccgtga acggtttcgc ccgggccggt gcggtgacga tgctcggcgt
    12241 gatcgactcg atcatgtatc cgggcacgga ccagttccag cgcttccagt atccgtcggc
    12301 ccttccgcgc gaattgcagt cgggcgcgga agaggttgcg cggcgggcgc tcgccgcggt
    12361 cgggttcacg cacggcatgt tcaacgtgga actgcgcctg tgcacgtcca ccgggctcgc
    12421 caaggtgatc gagatcaatc cgcgggcagc ggggcagttc tacgacctct tcgagcgcgt
    12481 ggacggctac aacctcttcg atgcgctcgt cgccttggag acgggagggg aaccggcaat
    12541 ccgccaccgg caggggcgcg acgcattcgc agcgagcttc gtgctgcgcg acctggaggg
    12601 cgcggggctc ggccgctggc cggcccgggg cgagatcggc cgtttgcgcg cgcgccatcc
    12661 cgaggcgacg atcatgttct acccgaagcg cggggccgac cttcgccgcg agatgaaatg
    12721 gctgggaagc taccggtacg gcgtggtgaa tctcggcgcg ccgacgctgg gggagctgtt
    12781 cgcgaactac ggccggatcc atcgcgacat cgcgttccat ccccgcggcc aggaatcctc
    12841 gcgcgtcgac gagatgctcg gggaagcagc cgccgactag cgcgggaaca ggcggcttcg
    12901 tccgcggctc catcgccctg ccccgggcga agcgttccgg aattcactcg cgaagccatg
    12961 ccgcgtcgaa tagcgggttc gtcctcgagg tgaggacgtg gtcgcgccac gccccgtcga
    13021 tgaagaggta ctcgcgcgcg tagccctcgc gcaggaatcc gagccgctcg agcaggcggc
    13081 cgctgcgcgc gttttcgggc cggtagttgg ccatgatgcg gtgcaggcga agctcgccga
    13141 aggcgtaggc gattgtcgcg ccgagcgcct cgtgcatgag cccgtggccc tcgtgccggc
    13201 gatcgacctg gtagcccagc acgcaggcgc ggaacggtcc cttcacgatc tgcgtgaaat
    13261 tgcaggtccc gatgacctcc ggcgccgccg cattcggcga gagcatcgcc cagcgcgccg
    13321 ttgtgccttc cctgaactcc ttctcgaata cccgcaggcg cttcgcccag tgatcctcgc
    13381 cgaagccatc ctgcggtggc gggggggacc accggtcgag atgtcccgcg aagttatccg
    13441 cgtagaagcg ggcgaacgct agttcgagcc ccgggcgcgc gatgaccagc ctgagccgcg
    13501 cggtctcgat ctcggggaag tccacgcagg ccgcgcctcg cgggcgctca ggcagacagg
    13561 acgcgccagt gttgcggcca gccctcggtg ggggagcgct tgaagccgct cttggcgaac
    13621 aggtgccagc ggccgatcac gtaggcgatg agcgtcgtgg cataggcgtt ggcgtcccag
    13681 tcggccggca gctggcccgc gctgaccgcg aggcgcgtgc tctggcgaat gctcgcctcg
    13741 aggcggtcga cgagctggtt cacgcgcagc tgcaggcgct cgtcctcgat cgccagcgca
    13801 tctcccgtga gcacgcgcgt catgccgggg ttcttctgcg cgaaggcgag cagcgcgacg
    13861 agcatggact cgacctgctt catcgcgtcc gcctcgtcgg actggatgcg gttgacgagc
    13921 gtgaacagcg tctgctcgat gaattcgatg agcccctcgt acatctgggc cttgctggcg
    13981 aagtggcggt acagcgctgc ctccgacacg tccaggcgcg ccgcgagcag cgccgtcgtc
    14041 acgcgttccg ccttgggctc ctcgagcatc gaggcgagca tctgcaggat ctgggccttg
    14101 cgctccccgg tcttcgccat gggtctccct tcgtcagcgg cgaccgccga ggatcgaccc
    14161 cagcacgccg cggatgatgc gtcgcccgac ttcgcttccc atcgcgcggg cggcgctctt
    14221 ggccgcggcc tcgatggccg tctcccgcgt cctgcccttc gccgcgtcgc ccccgggaag
    14281 tccgagcttg cccaggattc cgcccagcac gctgccgccc gtctcttcgc cctgcgcacg
    14341 ctcgcccgcg cgggcggcga gcttctcgta ggccgactcc cggtcgacgg ccttctcgta
    14401 ctggcccgcg accagcgagg attcgatcgc cttgcgtcgc tcgtcgggcg tgatcggccc
    14461 gatgcgcgag cgtggcggaa tgacgaaggc acgctccacg gggaacggcc tgcccttctc
    14521 gtcgaggaac gacaccaacg cctcgccgac gcccagctcg gtgatggcct tctcgacatc
    14581 gagcttcggg ttcgaccgga acgtggtggc ggcggccttc acagcctgct ggtcgcgcgg
    14641 cgtataggcg cgaagcgcat gctgcacgcg gttgccgagc tgccccagga ccgtatccgg
    14701 gacatcgagc ggattctgcg tgacgaacca gacgcccaca cccttcgagc gcaccaggcg
    14761 caccacctgt tcgatgcggt cgagcatttc cctgggcgcg tcgctgaaga gcaggtgcgc
    14821 ctcgtcgaag aagaagacga gcttcggctt ctcggggtcg cccacctcgg gcagctgctc
    14881 gaaaagctcc gagagcagcc acagcagcat cgtcgcgtag acgcgcgggc tggcgaggag
    14941 cttgtcggcg gcgaggatgt tcacgacgcc cttgccgtcg agggtctgca tgaggtcgtc
    15001 gagctcgagc accggctcgc cgaagaactt ctcgccgccc tgggattcga gcgcgaggag
    15061 gccgcgctgt atcgcgccga tgctcgccgt cgagatgttg ccgtaccggg tccggaactg
    15121 cgcggcgttg tctcccgcat gctgcaggag cgcgcgcagg tccttgagat cgaggagcgc
    15181 gaggccgtcg tcgtcggcga tcttgaacac ggcggcgagg acgccggcct gcgtctcgtt
    15241 gaggtcgagg atgcgcgcga gcagaagcgg ccccatttcc gtaaccgtgg cgcgc
//
LOCUS       DASUEV010000130         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_39755, whole genome shotgun sequence.
ACCESSION   DASUEV010000130 DASUEV010000000
VERSION     DASUEV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39755"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..126
                     /locus_tag="VFV55_04335"
     CDS             4..126
                     /locus_tag="VFV55_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX4943557.1"
                     /translation="MRKLEKEMLEAARNLEFERAAHLRDELRVARERLMEVGGG"
     gene            119..628
                     /locus_tag="VFV55_04340"
     CDS             119..628
                     /locus_tag="VFV55_04340"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX4943558.1"
                     /translation="MAERKAGVLFVCTGNICRSPTAEGVFRRLAGERGMLEKMLVDSA
                     GTHDYHVGEAPDPRAVEHAARRGYDLAPLRARQVTRHDFDAFDRILAMDRSHLRQLQA
                     IAPPGAPARISMFLDHSQRWRGLDVPDPYYGGSRGFEEVLDMVEEASALLLEEILAEI
                     PAELPRRRR"
     gene            625..1194
                     /locus_tag="VFV55_04345"
     CDS             625..1194
                     /locus_tag="VFV55_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX4943559.1"
                     /translation="MSKPPASRFPVPELSELPEDMRERINKVQEKAGFVPNVFVALAH
                     RPDEFRAFFAYHDALMLRAGGLSKAEREMIVVATSAANQCLYCIVAHGAILRAYSKRP
                     LIADQVAGNYLKADLSPRERAILDFAMKVALEAHRVEEADFVPLREHGLSDEDVWDVG
                     AIAAFFALSNRMANLTAMRPNDEFYTMAR"
     gene            complement(1207..1578)
                     /locus_tag="VFV55_04350"
     CDS             complement(1207..1578)
                     /locus_tag="VFV55_04350"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4943560.1"
                     /translation="MLESIGQIAVNVKDTARARAFYRDVLGLKHLFDAGPTLAFFQCG
                     GVRLMLSPASKPEYDHPGSIIYYKVADLTATHAAILAKGATFEAAPHLVATMPDHELW
                     MAFLRDSEGNLLALMEEKRPA"
     gene            complement(1597..2199)
                     /locus_tag="VFV55_04355"
     CDS             complement(1597..2199)
                     /locus_tag="VFV55_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX4943561.1"
                     /translation="MTAPIDFWFDFSSPYGYIAAELIDDVAARHGRTVNWHPILLGPI
                     FKAVGTAPLTMLPIKGEYSKLDFARTARFHKVPFRLPEKFPVGTQVAARAFYLIADRD
                     PARARDFARRVFRAYFAEGRDVADPAVVIELAGAAGVDTAALADPLKGEAIKERMKSE
                     VDGAMKAGVFGSPHFIVDGEPFWGVDRIPAMEEWMRTGGW"
     gene            complement(2206..>4648)
                     /locus_tag="VFV55_04360"
     CDS             complement(2206..>4648)
                     /locus_tag="VFV55_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX4943562.1"
                     /translation="ARTAGIGRSIEEMQWDLNYLLQLWNAIETAAKEEKGPCLIYLES
                     SLVVRAIRDLFQPDIGEILVDTPHIHEQALAFMSTVMPANVNKVKLYRDDVPLFSRFQ
                     IEHQIESAYRRDVTLPSGGAIVIDHTEALVSVDVNSARATRGHDIEQTAFNTNLEAAD
                     EIARQLRLRDLGGLIVIDFIDMESVKNQREVENRLRDALRHDRARVQVGKISRFGLLE
                     LSRQRLQPSLEETTHSACPRCHGTGFIRDTASTALHVLRILQEEAMKESTAAVHVQVP
                     VDVGTYLLNEKRSEIIKIEARLKVGVILIPNPALETPNYHIERLRHDSPLLDAQVPSY
                     EMVGIPEEEEDERRPQRGAQPKDKEEKPARPEPLVKGITPDQPAPNVPAPDVVAAQAA
                     AAQSAPVPEKATGGLIERIFGWFRKPENEPVASIPAPVTAARPAEGIIRKERDDKRGG
                     RGDGKRGDSRRGEGRGRGEGRNEPRGEARGEVRGEARGEARGEARGEARGEARGEARG
                     EARGEGRGEGRARGEGRGEGRGRGEGRNEPRGEGRTGDRGEGRQDGRRRGQQQAGEPR
                     AQREAEAPLPGALEPVAALTAHPVIPAEAPNGDGEGEGGRRRRRGRGRGRGDRTERAP
                     ETMAELRAEFPAEPETEAVPPLRSEPRAEPPRHTEPSAEPPRHAEPRSAPMPAATREV
                     PVAVPAAAPPDEPPVMATPIPRAPQVIASFSAPPATYQVVSEHDKVAEEEPHRPVRRH
                     REEGSGPAPAEPLQLVETASDKVVAQPVFEDDAPRRPVRRRHRAPAPAAPAEPLQLVE
                     TASDVQSTEGNSPQA"
BASE COUNT          630 a         1572 c         1646 g          800 t
ORIGIN      
        1 cgcgtgcgca agctggaaaa ggagatgctg gaggcggcgc gcaatctcga gttcgagcgc
       61 gcggcgcacc tgcgggacga attgcgcgtg gctcgcgaga ggctcatgga ggtgggcggt
      121 ggctgagcgc aaggccggcg ttctattcgt ctgcacggga aacatctgcc gctcgcccac
      181 ggccgaggga gtcttccgcc gactggccgg ggagcgcggc atgctggaga agatgctcgt
      241 cgattccgcc ggcacccacg actaccacgt gggcgaggcg cccgacccgc gcgccgtcga
      301 gcacgcggcg cggcgcggct acgatctcgc gccgctgcgg gcgcgccagg tcacgcgcca
      361 tgatttcgac gcgttcgacc gcatcctcgc catggaccgc agccacctgc gacaattgca
      421 ggcgatcgcg ccgcccggag cgccggcgcg gatctcgatg ttcctcgatc attcgcaacg
      481 ctggcgcgga ctcgacgtgc cggacccgta ctatggggga agccgcgggt tcgaggaggt
      541 gctggacatg gtggaggaag cctcggcgct gctcctcgag gaaattctcg ccgaaatccc
      601 cgctgaactg ccaaggagaa gacgatgagc aagccccccg caagcaggtt tcccgtcccc
      661 gagctctccg agctgcccga ggacatgcgc gaacgaatca acaaggtgca ggagaaggcg
      721 ggattcgtgc ccaatgtctt cgtcgcgctg gcgcaccgcc ccgacgagtt ccgcgccttc
      781 ttcgcctacc acgacgcgct gatgctgcgc gcgggcggcc tgagcaaggc ggaaagggaa
      841 atgatcgtcg tcgccacctc ggccgcgaac cagtgcctgt actgcatcgt ggcccatggc
      901 gcgatcctgc gcgcgtactc caagcgcccg ctcatcgccg accaggtggc gggcaactac
      961 ctcaaggccg atctttcgcc gcgcgaacgg gcgatcctcg acttcgcgat gaaggtggcc
     1021 ctggaggcgc accgggtgga ggaggccgat ttcgtcccgc tgcgggagca cgggctgtcc
     1081 gacgaggacg tgtgggacgt gggcgccatt gccgcgttct ttgcgctttc caaccgcatg
     1141 gccaacctga ccgccatgcg gccgaacgac gagttctaca cgatggcgcg gtagcggtgc
     1201 cgcgattcag gcggggcgct tttcctccat gagcgcgagg aggttgcctt cggagtcgcg
     1261 caggaaagcc atccacagct cgtggtccgg catggtggcc accaggtgcg gcgcggcctc
     1321 gaaggtcgcc cctttcgcga ggatcgccgc gtgtgtcgcc gtgaggtccg cgaccttgta
     1381 gtagatgatc gatccggggt ggtcgtactc gggcttcgag gcgggcgaga gcatcagccg
     1441 cacgccgccg cactggaaga aggcgagcgt cgggcccgcg tcgaacaggt gcttcaggcc
     1501 cagcacgtcg cggtaaaagg cccgcgcccg ggccgtgtcc ttcacgttca cggcgatctg
     1561 gccgatcgat tcgagcacgt cgttcccctt ctccggctac cagccgccgg tgcgcatcca
     1621 ctcttccatc gcgggaatgc ggtccacgcc ccagaacggc tcgccgtcga cgatgaaatg
     1681 cggcgagccg aagacgcctg ccttcatggc gccgtcgacc tcggacttca tgcgctcctt
     1741 gatcgcctcg cccttgagcg gatccgcaag ggccgccgta tcgaccccgg ccgcgccggc
     1801 cagctcgatc accactgccg gatcggcgac gtcacgaccc tcggcgaaat aggcgcggaa
     1861 gacgcgcctc gcgaaatcgc gtgcccgagc cgggtcgcga tcggcgatga ggtagaacgc
     1921 gcgcgccgcg acctgcgtgc cgacgggaaa cttctccggc agccggaacg gcaccttgtg
     1981 gaagcgcgcc gtgcgcgcga agtcgagctt cgagtactcg cccttgatgg gcagcatcgt
     2041 gagcggcgcc gtgccgacgg ccttgaagat ggggccgagc aggatcgggt gccagttcac
     2101 ggttctcccg tgccgggcgg ctacatcgtc gatcagttcc gcggcgatgt aaccgtacgg
     2161 cgaggagaag tcgaaccaga aatcgatcgg agcggtcatg ccgcctcagg cctgcggcga
     2221 gttgccttcg gtcgactgga cgtcggacgc ggtctcgacg agctgtagcg gctcggcggg
     2281 cgctgccggg gcaggcgcgc gatggcggcg tcgcaccggc cggcgtggcg cgtcgtcctc
     2341 gaataccggt tgtgccacga ccttgtcgct ggccgtttca accagttgca gcggctcggc
     2401 cggggcgggg ccgctgcctt cctcgcgatg ccggcgaacc gggcgatgcg gttcctcttc
     2461 ggcgaccttg tcgtgctcgc tgaccacctg gtaggtggcg ggcggcgccg agaaggacgc
     2521 gatgacctgg ggcgcacgcg gaatcggcgt ggccatcacg ggcggttcat cgggcggtgc
     2581 ggccgcaggc actgcaaccg ggacttcccg ggttgcggcc ggcatcggtg ccgatcgggg
     2641 ttcggcgtgc cgcggcggtt ctgcacttgg ttcggtgtgc cgcggcggtt ctgctctcgg
     2701 ttcgctgcgc agcggcggca ccgcctccgt ttccggttcc gcggggaact cggcccgcag
     2761 ctcggccatg gtttccggtg cgcgctcggt ccggtcgccg cgaccgcggc cgcggcctcg
     2821 ccggcggcgc cggccgcctt caccttcgcc gtcaccattc ggtgcctcgg cggggatcac
     2881 gggatgcgcg gttagggcgg ccacgggctc gagcgcgccg gggaggggag cttccgcttc
     2941 cctttgcgcg cgaggttcgc ccgcttgctg ctggccgcgc cggcggccgt cctggcgtcc
     3001 ttcgccacgg tccccggttc gaccttcgcc acgcggctcg ttgcggcctt cgccgcgtcc
     3061 acgaccctca ccacggcctt ctccgcgggc gcggccttca ccgcggcctt caccacgtgc
     3121 ttcaccgcgt gcttctccac gtgcttcacc gcgagcttca ccgcgagctt cgccccgagc
     3181 ttcaccgcga gcttcgcctc gaacttcacc ccgtgcttcg ccccgcggct cgttgcggcc
     3241 ttcgcctcgc ccacgacctt caccgcgacg gctgtcgcct cgcttgccgt cgccgcggcc
     3301 gccccgcttg tcgtcacgct ccttgcggat gatgccttcg gccggccgtg ctgccgtgac
     3361 cggtgcgggg atggatgcga ccggctcgtt ctccggcttg cggaaccagc cgaagatgcg
     3421 ctcgatcaac ccgccggtcg ccttctccgg gactggcgcc gattgcgcgg ccgcggcctg
     3481 cgcggcgacg acgtccggcg ccggcacatt gggggccggc tgatcggggg tgatgccctt
     3541 cacgagcggc tcggggcgcg cgggcttttc ttccttgtcc ttgggctgcg cgccgcgctg
     3601 cgggcggcgt tcgtcctctt cctcttccgg gatgccgacc atctcgtagc tgggcacctg
     3661 ggcgtcgagg agcggcgagt catggcgcag gcgctcgatg tggtagttcg gcgtctcgag
     3721 ggcggggttc gggatgagga tcacgccgac cttgaggcgc gcctcgatct tgatgatctc
     3781 cgagcgcttc tcgttcagca ggtaggtgcc gacgtccacc ggcacctgca cgtgcacggc
     3841 ggccgtgctt tccttcatgg cctcctcctg caggatgcgc aggacgtgca gcgcggtcga
     3901 ggcggtgtcg cggatgaatc cggtgccgtg gcagcgcggg caggcggaat gggtggtctc
     3961 ctcgagggag ggctgcaggc gctgccggga gagctccagc aggccgaagc gcgagatctt
     4021 gccgacctgg acgcgggcac ggtcgtggcg cagcgcgtcg cgcaggcggt tctccacctc
     4081 gcgctggttc ttcacgcttt ccatgtcgat gaagtcgatg acgatgagcc cgcccaggtc
     4141 gcgcaggcgc aactggcggg cgatctcgtc cgcggcctcg aggttggtgt tgaacgccgt
     4201 ctgctcgatg tcgtgcccgc gcgtggcacg tgccgagttc acgtcgacgg acacgagggc
     4261 ttccgtgtgg tcgatcacga tcgcgccgcc ggagggcagg gtgacgtcgc gccggtaggc
     4321 gctctcgatc tggtgctcga tctggaagcg cgaaaagagc ggcacgtcgt cgcggtagag
     4381 cttcaccttg ttcacgttgg ccggcatcac ggtgctcatg aacgcgagcg cctgctcgtg
     4441 gatgtgcggc gtgtcgacga ggatctcgcc gatgtcgggc tggaagaggt cgcggatggc
     4501 gcgcactacc aggctcgact cgaggtagat gaggcagggg cccttctcct ccttggccgc
     4561 ggtctcgatg gcgttccaga gctgcagcag gtaattgagg tcccactgca tttcctcgat
     4621 gctgcggccg atacccgccg tgcgggcg
//
LOCUS       DASUEV010000131         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_39863, whole genome shotgun sequence.
ACCESSION   DASUEV010000131 DASUEV010000000
VERSION     DASUEV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39863"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFV55_04365"
     CDS             complement(<1..663)
                     /locus_tag="VFV55_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4943563.1"
                     /translation="MNRITQLALAVAIALGIAACGQKEEAKPKVEAKPAAPAGVTVTI
                     AHAGPLTGSIAHLGKDDENGVALAVQHANDKKLVIDGKPVTFKMVSEDDQADPKTGTT
                     VAQKLVDAKVAAVVGHLNSGVTIPASEIYNKAGIPVISGSATNPTLTERGLKTVFRTV
                     GRDDQQGPAIAAYIAGELKAKKVAIIDDKTAYGEGLANEVEKYLKGAKISIVGRERTT
                     DKE"
     gene            complement(833..1144)
                     /locus_tag="VFV55_04370"
     CDS             complement(833..1144)
                     /locus_tag="VFV55_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4943564.1"
                     /translation="MKKSYLVIPALGLAFAAAPALANLELATKSACTACHAVDKKLVG
                     PAYKDVAAKYKGNPKAEAMLIEKVKKGGVGTWGQVPMPPNANVKDDDVKTLVKWVLST
                     K"
     gene            complement(1159..>2622)
                     /locus_tag="VFV55_04375"
     CDS             complement(1159..>2622)
                     /locus_tag="VFV55_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX4943565.1"
                     /translation="KFPHPDTIETCLRHYAASSASSSLDMATTTLAKMAEGGIFDQLG
                     GGFARYSVDNDWAIPHFEKMLYDNGQLIRLYADAWAITGDSLFARTVEETAAWVMREM
                     QSPEGGYYSSLDADSEGEEGKFYVWTREEVQALLTTQEKAVAIPHFGFDRGPNFEGHA
                     WNPVVATRIPAIAATLGIAEADARSQLASARAKLFAARERRIRPGRDDKILTSWNALM
                     IGGMAHAARVFDRPDWLASARRALDFIRSTMWKDERLLATHKDGRTHLNAYLDDYAFL
                     LAALLEVLQADFRADDLAWAEAVGNALIESFQDTDGGGFFFTSHDHERLIHRPKPGQD
                     NATPSGNGVAAWALNRLCFLSGETSFQRAAEGTLALFWPAMERSPAGFGSLLATLEET
                     LKPPTTVIVNGPESGFAPWREALRSDYRPDTMVLFVPGTATPLPPPLAKPAGAAVNAW
                     VCEGVTCMAPLAEPGQLRETLKDPKITGCASTPNRSA"
BASE COUNT          374 a          812 c          940 g          496 t
ORIGIN      
        1 ttccttgtcg gtggtgcgct cgcggccgac gatcgaaatc ttggcgccct tcaggtactt
       61 ctcgacctcg ttggcgagcc cctcgccgta ggcggtcttg tcgtcgatga tggcgacctt
      121 cttcgccttg agttcgcccg cgatgtaggc ggcgatggcc gggccctgct ggtcgtcgcg
      181 cccgacggtg cggaacaccg tcttgagtcc gcgctcggtg agcgtggggt tggtggcgga
      241 acccgaaatc accgggatgc cggccttgtt gtagatctcg gaggcgggaa tcgtcacgcc
      301 cgagttgagg tggcccacga ccgcggcgac cttggcgtcc accagcttct gtgcgaccgt
      361 tgtgccggtc ttcgggtccg cctggtcgtc ctcgctcacc atcttgaagg tgacgggttt
      421 gccgtcgatc acgagcttct tgtcgttggc gtgctgcacc gcgagtgcca ccccgttctc
      481 gtcgtccttg ccgaggtgcg cgatggaacc ggtgagcgga cccgcgtggg cgatggtgac
      541 ggtgacaccg gccggggcgg cgggcttcgc ttcgaccttg ggcttggctt cttccttctg
      601 gccgcaggcc gcgatgccga gggcgatggc gacggccagg gcaagctggg tgatacggtt
      661 catcgaggtg tctccttgtg ggatgtgggg ggcgggcgct tcggcccagc cgggcggggg
      721 tgtgaagcgc gcaattatag cgggtgccac aaaagggaaa agggtcaggt tactttcggt
      781 gtctgacacc gaaagtaacc tgaccctttt cccgcgtaag cgggtgctta cattacttcg
      841 tggagaggac ccacttcacg agcgtcttga cgtcatcgtc cttcacgttg gcattcgggg
      901 gcatcggaac ctggccccag gtgcccacgc cgcccttctt caccttctcg atgagcatcg
      961 cctcggcctt ggggttgccc ttgtacttgg ccgcgacgtc cttgtaggcg gggccgacga
     1021 gcttcttgtc cacggcgtgg caggcggtgc aggcggattt ggtcgccagc tccagattgg
     1081 ccagggccgg cgcggcagca aacgcgagtc caagggccgg gatgacgaga taggacttct
     1141 tcatgatcga gcttctcctt aggcggaacg gttgggggtt gaggcgcaac ccgtgatttt
     1201 cgggtctttc agcgtttcgc gcaattgccc cggctccgcg aggggagcca tgcatgtaac
     1261 gccctcgcac acccatgcgt tgaccgccgc gccggcgggc tttgccaggg gcgggggcag
     1321 gggggttgcg gtgccgggca cgaacaacac catcgtgtcc ggccggtaat cgcttcgcag
     1381 ggcctcgcgc cagggggcga atcccgactc ggggccgttc acgatgacgg tcgttggcgg
     1441 cttgagcgtc tcctccagcg tcgccaggag gctgccgaaa ccggcggggc ttcgctccat
     1501 cgccggccag aagagcgcga gcgtcccttc cgcggcccgc tggaagctcg tctcgcccga
     1561 aaggaagcaa aggcgattca gcgcccacgc cgccaccccg ttgcccgagg gcgtcgcatt
     1621 gtcctgcccc ggcttgggcc ggtggatgag gcgctcgtgg tcgtgggagg tgaagaagaa
     1681 gccgccgccg tccgtgtcct ggaacgattc gatgagcgcg ttgcccaccg cctcggccca
     1741 ggcgaggtca tcggcacgga agtcggcctg cagcacttcg agcagggcgg ccagtaggaa
     1801 cgcgtaatcg tcgaggtagg cgttgaggtg ggtccggccg tccttgtggg tggcaagaag
     1861 ccgctcgtcc ttccacatcg tcgagcgtat gaaatcgaga gcgcggcggg cggaggcgag
     1921 ccagtcgggc cggtcgaaga cgcgggcggc gtgcgccatg ccgccgatca tgagcgcgtt
     1981 ccacgacgtg aggatcttgt cgtcgcgtcc cggccggatg cgccgttcgc gggcggcgaa
     2041 gagcttcgcg cgcgccgagg cgagttgcga gcgcgcgtcg gcctcggcga tgcccagcgt
     2101 ggcggcaatg gcggggatcc gcgtcgcgac gaccggattc cacgcgtggc cttcgaagtt
     2161 ggggccgcgg tcgaagccga agtgcgggat ggcgacggcc ttctcctgcg tcgtgaggag
     2221 ggcttgcacc tcctcgcgcg tccatacgta gaacttgccc tcctcgccct cggaatcggc
     2281 gtcgagcgag gagtaatagc ccccctcggg cgactgcatc tcgcgcatca cccacgcggc
     2341 cgtttcctcg acggtgcgtg cgaaaaggga atcgcccgtg atggcccacg cgtcggcgta
     2401 gaggcggatg agctgcccgt tgtcgtagag catcttctcg aagtgcggga tggcccagtc
     2461 gttgtccacg ctgtagcgcg cgaagccgcc gcccagctgg tcgaagattc cgccctcggc
     2521 catcttcgcg agcgtcgtcg tggccatgtc gagggacgag gacgcgctgc tggcagcgta
     2581 gtggcgcagg cacgtctcga tcgtgtcggg gtgcgggaac tt
//
LOCUS       DASUEV010000132         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_148114, whole genome shotgun sequence.
ACCESSION   DASUEV010000132 DASUEV010000000
VERSION     DASUEV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148114"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VFV55_04380"
     CDS             <1..1693
                     /locus_tag="VFV55_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX4943566.1"
                     /translation="SDQWFVKMEGFAKAGLDAVASGRTRFVPDMWATVYNQWLENIQD
                     WCISRQLWWGHQIPAWYAEDGTAFVGRNFEEASAKAKAAGTSIAPEARDPDVLDTWFS
                     SAFVPFSTLGWPDGAAMAKERPFYLPSSVLVTGFDIIFFWVARMIMTTLHFTGEVPFR
                     DVFINAIVRDAEGQKMSKSKGNTIDPLDVIDGITLADLVDKSTQGLMLPAHRQAAEKR
                     IKRDYPEGIASYGADALRFTFAALSTHGRTLNFDLKRCEGYRSFCNKLWNATRFVLMN
                     CEGKDTGLDPAAPVELSFADRWIVSRLQRAEGEVAQGYEEYRFDMSARALYEFVWDEY
                     CDWYLELAKVQLQSGSEAAQRGTRRTLIRVLETTLRLAHPVIPFITEELWQKVAPLAG
                     RTGDTVMLARWPQAQPEKIDAAAEHDIAVLKELTNAARNLRSEMGLAPGQRVPAFVQA
                     GPEVAVHLPYIQALARLSEAKVVATLPDADSPVAATGSGKLMLHVEIDRAAERERLAK
                     EAARLDGEVVKAETKLGNASFVERAPAAVVEQEKKRLADFRAKLDDIRVQLARLA"
     gene            1701..2972
                     /locus_tag="VFV55_04385"
     CDS             1701..2972
                     /locus_tag="VFV55_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4943567.1"
                     /translation="MPLPRSLRALASPNFRRYYAGQAVSMIGTWVQSLALMWVAYGLS
                     GSTLFTGLVGFLNSVPYLVLSPFAGVLGDRLDRRRILLTVLTLLFLQSLALAALSGLG
                     MLTMTQLAALALFAGVVNSFETPTRQSFFVQLLDNRDDLPNAIALNSVLMNGARLVGP
                     SLGGLVIAAWGETACFAINAASYLFVIGALGRTHAVRASPPRPPSHPLADLADGWRYA
                     MGFVPIRRMLFMLATISITIGPYSSLMPAIAVKTFGEGAQLVGLFIGAVGFGAVVAAV
                     GLARRPSVRGLAKWIGVSAILAGAGASGFCFSRSIPLSAFLMATAGFGMFMTAASCNT
                     IVQSIVEEDKRGRVMSYYTMFFIGSLPIGHLAAGALAERIGAPYTFLAGGIACSLVGA
                     VFAFRLPSFRDHLRPVYVERGIIPPTGEPPQ"
     gene            2969..3754
                     /locus_tag="VFV55_04390"
     CDS             2969..3754
                     /locus_tag="VFV55_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX4943568.1"
                     /translation="MTDTVLLERRGSAAIITLNRPDKLNALDGALMQDLDRVTREVAE
                     DSAIRAVMLRGAGRAFMAGGDVAFFHAHLDTLAQRFQEIGQGFHASVKRLRTMDKPVL
                     ACVHGAVAGGGLSVMLACDLAIASRDAQLSLAYANIGTSPDGGSSFTLPRIVGLRKAL
                     ELALLGERLDADAACSLGLVNWVVEPAELEARAAAILERLAAGPTFAYARTKALFNET
                     WERPMTVQLDAELASFARCVATHDFAEGTGAFVEKRKPVFTGK"
     gene            3760..4581
                     /locus_tag="VFV55_04395"
     CDS             3760..4581
                     /locus_tag="VFV55_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4943569.1"
                     /translation="MATLAGKTLFITGASRGIGREIALRAARDGANVAIAAKTTEPHP
                     KLKGTIHTVAAEVEAAGGKALAIVLDVRDEAAIAAGVESAARAFGGIDILVNNASAIS
                     LTPTLQTPARRFDLMMGVNVRATFLCSQACIPYLRKASNPHILNLAPPLAMKAKWFAG
                     HTAYTISKYGMSLCTLGMAEELRPEGIAVNSLWPATTIATAAVEVHFPPEILAASRHP
                     AIMADAAYAILTRPSREATGNFFLDEAVLRAAGVTDFDRYAITPGVPLFRDLFVD"
BASE COUNT          668 a         1667 c         1523 g          756 t
ORIGIN      
        1 ctccgaccag tggttcgtga agatggaagg gttcgcgaag gccgggctcg acgccgtggc
       61 atcgggacgc acgcggttcg tgccggacat gtgggccacc gtgtacaacc agtggctcga
      121 gaacatccag gactggtgca tctcgcgcca gctttggtgg gggcaccaga tcccggcgtg
      181 gtacgccgag gacgggacgg ctttcgtggg gcgcaatttc gaagaagcgt cggcaaaggc
      241 aaaggcagcc ggcacttcga tcgctcccga agcgcgcgac cccgatgtcc tcgatacctg
      301 gttctcctcg gccttcgtgc ccttctcgac gctcggctgg cccgacgggg ccgcgatggc
      361 gaaggaaagg cccttctacc tgccctcctc cgtcctcgtc accggcttcg acatcatctt
      421 cttctgggtg gcgcgcatga tcatgacgac gctccacttc accggcgagg tgccgttccg
      481 cgacgtattc atcaacgcca tcgtgcgcga cgccgagggc cagaagatgt cgaagtccaa
      541 gggcaacacc atcgaccctc tcgacgtgat cgacggcatc acgctggccg acctggtgga
      601 caagtcgacg caaggcctca tgctcccggc gcaccgccag gcggccgaga agcgcatcaa
      661 gcgcgactat cccgagggca tcgcctccta tggcgcggac gcgctgcgct tcaccttcgc
      721 cgccctgtcc acgcacgggc gcacgctcaa cttcgacctc aagcgctgcg agggctaccg
      781 cagtttctgc aacaagctct ggaacgccac gcgcttcgtg ctgatgaact gcgagggcaa
      841 ggacacgggc ctcgatcctg ccgcgcccgt cgagctctcc ttcgccgacc ggtggatcgt
      901 ctcgcgcctg cagcgcgcgg agggcgaagt cgcgcagggc tacgaggaat accgcttcga
      961 catgtcggcg cgcgcgcttt acgagttcgt gtgggacgag tactgcgact ggtacctcga
     1021 gctcgccaag gtgcagctcc agtccgggag cgaggcggcg cagcgtggca cgcgccgcac
     1081 gctcatccgg gtgctcgaga ccacgcttcg cctcgcccat cccgtgatcc cgttcatcac
     1141 cgaggagctt tggcagaagg tggcgccgct ggcgggccgc acgggcgaca ccgtgatgct
     1201 cgcgcgctgg ccgcaagcgc agccggagaa gatcgacgca gcggcggaac acgacatcgc
     1261 cgtgctcaag gaactcacca acgcggcgcg caacctgcgc tccgagatgg gcctcgctcc
     1321 ggggcagcgc gtgccggcct tcgtgcaggc tggccccgag gttgccgtcc accttcccta
     1381 tatccaggcc ctcgcgcggc tctcggaggc gaaggtggtc gcgactcttc ccgacgcgga
     1441 ctctcccgtg gcagcgaccg gtagcggcaa gctcatgctg cacgtcgaga tcgaccgtgc
     1501 ggccgaacgc gagcggctgg cgaaagaggc ggcgcggctc gatggcgagg tcgtgaaagc
     1561 ggagaccaag ctcggcaatg cctcgtttgt cgagcgcgcc ccggccgccg tcgtggagca
     1621 ggaaaagaag cggctcgcgg atttccgcgc caagctcgac gacatccggg tccagctcgc
     1681 gcgactggcc tgaccggccc gtgccccttc cgcgcagcct ccgggcgctc gcctcgccca
     1741 acttccgccg ctactacgcc gggcaggcgg tgtcgatgat cggcacctgg gtgcagtccc
     1801 tggcgctcat gtgggtcgcg tacggcctgt ccggctccac gttgttcacc gggctcgtgg
     1861 gcttcctcaa ctccgttccg tacctcgtgc tctcgccgtt cgccggcgtg ctgggcgacc
     1921 gcttggaccg gcgccgcatc ctgctcacgg tgctcacgct gctcttcctg cagtccctgg
     1981 cgctggccgc cctttcggga ttggggatgc tgaccatgac ccagctcgca gcgcttgcgc
     2041 tcttcgccgg cgtggtcaat tccttcgaga cgcccacccg gcaatccttc ttcgtgcagt
     2101 tgctcgacaa ccgggacgac ctgccgaacg cgatcgcgct caattccgtc ctgatgaacg
     2161 gcgcccgcct ggtgggcccg tcgctgggcg gactggtcat cgcggcctgg ggcgagacgg
     2221 cctgcttcgc gatcaacgcg gcaagctacc tgttcgtgat cggcgccctg ggccgcacgc
     2281 acgccgtgcg cgcctcgccg ccgcgtcccc cgagccaccc gctggccgac ctcgccgacg
     2341 gctggcgtta cgccatgggc ttcgtcccga tccgtcgcat gctcttcatg ctcgccacga
     2401 taagcatcac catcggcccc tattcgtcgc tcatgccggc catcgccgtg aagaccttcg
     2461 gcgagggcgc gcagctggtc ggcctgttca tcggcgccgt gggcttcggg gccgtcgtgg
     2521 ccgcggtggg ccttgcgcgg cgcccttcgg tgcgcgggct cgcgaagtgg atcggcgttt
     2581 ccgccatcct ggcgggcgcg ggcgcatcgg gcttctgttt ctcgcgctcc atcccgctgt
     2641 cggcgttcct catggcgacg gcgggcttcg gcatgttcat gaccgccgcc tcgtgcaaca
     2701 ccatcgtcca gagcatcgtc gaggaggaca agcgcgggcg cgtgatgagc tactacacga
     2761 tgttcttcat cgggtcgctg cccatcgggc acctcgccgc aggtgccctg gccgagcgga
     2821 tcggcgctcc gtacaccttc ctggccggcg gcatcgcgtg ctcgctggtc ggggcggtct
     2881 tcgccttccg cctgccgtcc ttccgcgacc acctgcgccc cgtctatgtc gagcgcggta
     2941 tcatcccgcc caccggagag cccccccaat gaccgatacc gtcctgctcg aacgccgcgg
     3001 ctcggcggcc atcatcaccc tgaaccggcc cgacaagctc aatgcgctcg acggcgccct
     3061 catgcaggac ctggaccggg tcacgcgcga ggttgccgag gattcagcca tacgcgcggt
     3121 gatgctgcgc ggcgccggcc gcgcgttcat ggcgggcggc gacgtcgcct tcttccacgc
     3181 gcacctcgat acgctggcgc agcgcttcca ggagatcggc cagggcttcc acgcctccgt
     3241 gaagcgcctg cgcacgatgg acaagccggt gctcgcgtgc gtgcacggcg ccgtggccgg
     3301 cggaggcctg tcggtgatgc tcgcctgcga tctcgccatc gcctcgcgcg acgcgcagtt
     3361 gtccctggcc tacgccaaca tcggcacctc ccccgatgga ggcagctcct tcacgctgcc
     3421 gcgcatcgtc ggcctgcgga aggcgctcga gctcgccctt ctcggcgagc gcctcgacgc
     3481 cgacgcggcc tgttcgctcg gcctcgtgaa ctgggtcgtc gagccggcgg aactggaggc
     3541 gcgcgccgcg gcgatcctcg agcggctcgc ggccggtccc accttcgcct acgcgaggac
     3601 caaggcgctg ttcaacgaga cgtgggagcg ccccatgacc gtgcagctcg acgccgagct
     3661 ggcctcgttt gcgcgctgcg tcgcgacgca cgacttcgcg gagggcacgg gcgccttcgt
     3721 cgagaagcgc aagcccgtgt tcacggggaa gtgaatgcga tggccacgct ggcgggcaag
     3781 acactcttca ttaccggcgc ctcgcgcggc atcggccgcg agatcgcgct acgcgccgcg
     3841 cgcgacgggg ccaacgtggc gatcgcggcc aagacgacgg agccgcaccc gaagctcaag
     3901 ggcaccatcc acacggtcgc cgccgaggtc gaagccgcgg ggggcaaggc gctcgccatc
     3961 gtcctcgacg tgcgcgacga agcggccatt gccgcgggcg tcgaatcggc ggcgcgtgcc
     4021 ttcggcggca tcgacatcct ggtgaacaac gcgagcgcca tcagcctcac gccgacgctg
     4081 cagaccccgg cccggcgctt cgacctgatg atgggcgtga acgtgcgcgc gaccttcctg
     4141 tgctcgcagg cgtgcatccc ttacctgcgc aaggcatcga acccgcacat cctgaacctc
     4201 gccccgccgc tggcgatgaa ggcgaagtgg ttcgccggcc acacggcgta caccatctcc
     4261 aagtacggca tgagcctgtg cacgctcggc atggccgagg aactccggcc cgaggggatc
     4321 gcggtgaact cgctgtggcc ggccacgacg atcgcgacag cggcggtgga ggtccacttc
     4381 ccgccggaga tcctggccgc cagccgccac ccggccatca tggcggatgc ggcgtacgcc
     4441 atcctcaccc ggccgagccg ggaggcgacg gggaacttct tcctcgacga ggccgtgctg
     4501 cgcgcggccg gcgtgacgga tttcgaccgc tacgccatca cgccgggcgt tcccctgttc
     4561 agggatctgt tcgtcgatta gcagtgcgcg cgccgtggca ttccccgccg gcgc
//
LOCUS       DASUEV010000133         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_957107, whole genome shotgun sequence.
ACCESSION   DASUEV010000133 DASUEV010000000
VERSION     DASUEV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957107"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFV55_04400"
     CDS             <1..640
                     /locus_tag="VFV55_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX4943570.1"
                     /translation="LDSKNPALQAMIEAAPQLLDHLGEGAARHFDETRRLLSDEGVEF
                     TVNPRLVRGMDYYNRTVFEWVTDRIGAQSTIAGGGRYDGLFAMLGGKPTPACGFGLGM
                     ERIILTMQAFGRSAAQEPDAFLVHAGEAASRWAWRVGERLRDEGHACVLGAGGSFKSQ
                     MKKADASGARFAIILGDDEVAAGKFTLKPLRGEGEQVLVTPEEALVRLRRPS"
     gene            674..1306
                     /locus_tag="VFV55_04405"
     CDS             674..1306
                     /locus_tag="VFV55_04405"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4943571.1"
                     /translation="MSGSYDFEEQERIAAIHHWWEDNAKFVYAAIAAFVLAVGGWRGW
                     LYWSAVQSEDAAAMVAELAKARGDAKRVADIAQAVIDKHPRTFFASEAALVSAQASFE
                     AGDLATAQSRLEWVMANGRDEHRGIARIRLAAVLLDLKKYAEALQVLEANKDEAWAAM
                     AADLKGDILFAQGRLDEARAAYKLAIDKSDQRSPLRSVTELKLGALGGAQ"
     gene            1303..2436
                     /gene="bamB"
                     /locus_tag="VFV55_04410"
     CDS             1303..2436
                     /gene="bamB"
                     /locus_tag="VFV55_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEX4943572.1"
                     /translation="MKAAGRAAATACALALAGCGYFGLFVDAAKKPMPLAEIHATVTP
                     RVAWSASVGKAGGYKFSPKLEGNRIFAAAADGTITILDAQDGRVISRLDTGRKLSSGA
                     SGQGDLVIAGTIKGEVVAFDMAGKEKWVSNVGGEVLAPVVVAGKSAIARTGDGRVYAL
                     AFADGKRQWVYQRPTPALLLRAETSVVATPTNVVAGYPGGKLIALDLDDGKLTWEVTV
                     SLPRGSTELERVADVSGLPVIDAGRICAAAFQGKVACFDIQGGNTLWTRDLSSAVGLA
                     IDDKSVYVVDDQDNVHALARDSGASRWKQDKLLRRRLTAPVVVDGKVVVGDSLGYLHV
                     LAKDDGAFIGRIALDGGAVNAMLPANAGLIVQTAGGTVSFVRF"
BASE COUNT          423 a          896 c          944 g          388 t
ORIGIN      
        1 cctggattcc aagaaccccg ccctgcaggc gatgatcgag gcggcgccgc agctgctcga
       61 ccacctgggc gagggtgcgg cgcgccattt cgacgaaacc cggcgcctgc tttcggacga
      121 gggcgtggag ttcaccgtga acccgcgcct ggtgcgcggc atggactact acaaccgcac
      181 cgtgttcgag tgggtcacgg accgcatcgg cgcccagtcc acgatcgccg ggggagggcg
      241 ctacgacggc ctcttcgcga tgctgggcgg caagcccacg cccgcctgcg ggttcggcct
      301 gggcatggag cgcatcatcc tcaccatgca ggcgttcggg cgcagcgccg cgcaggagcc
      361 cgacgcgttc ctcgtgcacg cgggcgaggc ggcgtcgcgc tgggcgtggc gcgtgggcga
      421 gcgcctgcgc gacgaagggc acgcgtgcgt gctgggcgcc ggcggcagct tcaagtcgca
      481 gatgaagaag gccgacgcga gcggtgcccg cttcgctata atcctcggcg acgacgaggt
      541 cgccgccggc aagttcacgc tgaagccgct ccgcggcgaa ggcgagcagg tcctcgtcac
      601 ccccgaagag gcgctcgtgc gcctgcgccg cccctcctga agtcccccga accccctccc
      661 gaagcgaccc cccatgtccg gatcctacga tttcgaagag caggaaagaa ttgccgccat
      721 ccaccactgg tgggaggaca acgccaagtt cgtctacgcg gccatcgccg cattcgtgct
      781 ggcggtgggc ggctggcgcg gctggctgta ctggtccgcg gtgcagtccg aggatgccgc
      841 ggcgatggtg gccgagctcg ccaaggcgcg cggcgacgcg aagcgggtcg cggacatcgc
      901 gcaggcggtg atcgacaagc acccgcgcac gttcttcgcc tccgaggccg cgctcgtttc
      961 ggcgcaggcg agcttcgagg caggtgacct ggccaccgcg caatcgcgcc tggaatgggt
     1021 gatggcgaac ggccgcgacg agcaccgcgg catcgcgcgc atccgcctgg ccgccgtcct
     1081 gctcgacctc aagaagtacg ccgaggcgct gcaggtgctg gaggccaaca aggacgaagc
     1141 ctgggccgcg atggccgccg acctgaaggg cgacatcctg ttcgcgcagg gccgcctcga
     1201 cgaggcgcgc gccgcctaca agctcgccat cgacaagagc gatcagagaa gcccgttgcg
     1261 ttccgtcacg gaactcaagc tcggcgcgct cgggggagcg caatgaaagc cgcgggccgc
     1321 gccgccgcca cggcctgcgc cctggcgctg gccggctgcg gctacttcgg cctcttcgtc
     1381 gacgcggcga aaaagcccat gccgcttgcc gagatccacg cgacggtaac gcccagggtg
     1441 gcgtggagcg cctccgtggg caaggcgggc gggtacaagt tctcgccgaa gctggagggg
     1501 aaccgcatct tcgccgccgc cgccgacggc accatcacga tactcgacgc acaggacggc
     1561 cgcgtgatct cgcgcctgga caccgggcgc aagctctcct cgggcgcttc cggccagggc
     1621 gatctcgtca tcgccggcac gatcaagggc gaggtggtcg ccttcgacat ggccggcaag
     1681 gagaagtggg tctccaacgt gggcggcgaa gtcctcgcgc ccgtggtcgt ggcgggcaag
     1741 agcgcgatcg cgcgcaccgg cgacgggcgc gtctacgcgc ttgcgttcgc cgacggcaag
     1801 cgccagtggg tctaccagcg tccgaccccg gcgctgttgt tgcgcgccga aacctcggtc
     1861 gtcgccacac ccaccaacgt cgtcgcggga taccccggcg gcaagctcat cgccctcgac
     1921 ctggacgacg gcaagctcac ctgggaggtg accgtgagcc tgccgcgcgg ctcgacggag
     1981 ctcgagcggg tggctgacgt gtcggggctg ccggtgatcg acgccgggcg catctgcgcg
     2041 gcggcgttcc agggcaaggt ggcgtgcttc gacatccagg gcggcaacac gctctggacg
     2101 cgcgacctgt ccagcgcagt cggcctcgcg atcgacgaca agagcgtcta cgtcgtggac
     2161 gaccaggaca acgtgcatgc gctggcccgc gacagcggcg cctcgcgctg gaagcaggac
     2221 aagctgttgc gccgcaggct caccgcaccg gtcgtcgtgg acggcaaggt cgtggtgggc
     2281 gattcgctcg gctacctgca cgtgctcgcg aaggacgacg gcgcgttcat cggccgcatt
     2341 gcgctcgacg gcggcgcggt gaacgcgatg ctgccggcaa atgcgggcct catcgtgcag
     2401 acggccggag ggacggtctc cttcgtgcgc ttctgatcgc cggacgagca cggcggggat
     2461 cgaagcaagc gtctgcgttc gtcgatctgg cacgaccgtg atcgaagcgg gcgtcatggt
     2521 tcagggtgtg tggagtgttt tatttgggtt ttggccagat caacttgctt acggctgcga
     2581 cgctgccggt atttcgacat aggttcggcc agcgatgccc gacgcgaatt gacctggcca
     2641 aaacccaaat a
//
LOCUS       DASUEV010000134         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1626563, whole genome shotgun sequence.
ACCESSION   DASUEV010000134 DASUEV010000000
VERSION     DASUEV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626563"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFV55_04415"
     CDS             complement(<1..249)
                     /locus_tag="VFV55_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943573.1"
                     /translation="MSNALVLATAPVLAIDAGNTRVKWGLRVGEEWVARGAIATAGAA
                     RLGRDWPTMPPGTRALGSNVGGLAVQSLVYEACARHDLV"
     gene            complement(246..1220)
                     /locus_tag="VFV55_04420"
     CDS             complement(246..1220)
                     /locus_tag="VFV55_04420"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX4943574.1"
                     /translation="MGPLTLNALRLLADGHFHSGTEIARRLDRSRAALSESLKGAREL
                     GVEVFSVPGKGYRLARPIEFLDAHAIVSRLWPVASRVRLTLLDEVDSTSTHLASAAQA
                     GAPSGTCVAAEWQRAGRGRRGRSWQAGLGASLTFSLLWRFEQGPGHLAGLSLAVAVAV
                     VRALERAGVPGVGVKWPNDLVHDWKKLGGILVETSGETLGPSAAIIGIGINYRLGEAL
                     AGRIDQPATDVVSCGGAMPSRNALLAGLLAELVIALERFGRQGFAAFRDDWKARHVYA
                     GRRVTVHAGDKPAQEAQVLDVADDGALVVAFGGRTARLTSAEISLRPL"
     gene            complement(1221..2099)
                     /locus_tag="VFV55_04425"
     CDS             complement(1221..2099)
                     /locus_tag="VFV55_04425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF018583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX4943575.1"
                     /translation="MPVAKSFFQNTIALVYDFDGTLSPQPMQEYTVLPRMGIAPREFW
                     KRVNEEALATESDMMLVYMRHILESLDRQKIAVKREDFAKMASAIRYFPGVEGWFRRV
                     NDYVRKQSGGRVKLRHYVISAGQKEILDGVSIRRHFRRIYASEYHFNHHGVATFPKLL
                     VTDTVKTQFLFRINKGKEAVTESINEHMPEAERPIPFQNMIYVGDGMTDVPSMALTKK
                     NGGRTLAVYAKDDERGKATCVKLLEAGRADYVAQADYRKGSKLSRRMELLLDAIVADI
                     ACRREAFECRAEHRAG"
     gene            complement(2300..2581)
                     /locus_tag="VFV55_04430"
     CDS             complement(2300..2581)
                     /locus_tag="VFV55_04430"
                     /inference="COORDINATES: protein motif:HMM:NF022786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HEX4943576.1"
                     /translation="MTTQIPEDAKAFFDLANRFVALANDIGEEHGRPRAAAAILWAAS
                     RYGAFTWAMSGAPGKQSPEEALDLFAAQYRKMMEDNLARMLAEFTPPSS"
     gene            complement(2578..4008)
                     /locus_tag="VFV55_04435"
     CDS             complement(2578..4008)
                     /locus_tag="VFV55_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4943577.1"
                     /translation="MNDRTPFPAALAGELHAIVGDRGLVTAAADLEPFVTDWRGQFRG
                     RAALLVRPASTQELSKVVALLSAAGVGIVPQGGNTSLCGASVPDASGTQVIVSVSRMN
                     RVRAVDLDNDTITVEAGCILADLQRVAAQNDRFFPLSLAAEGSCEVGGNISTNAGGTQ
                     VLHYGNMREQVLGLEVVLPDGRVWDGLRGLRKDNTGYDLKHLFIGAEGTLGIVTAAVL
                     KLYPMPRAIATALVAVADPAAAVTLLGKLRAACGERVTGFELIARVCFDLVFRHITAS
                     RDPLPQGHPWYVLVELFDSSAGSGLAEMLETALGESAEAGLVQDAVVAASEAQRLELW
                     ALRENVSDAQKLEGVSVKHDVSVPVGCVPKLIEDGDRALQAAFPGIRTVAFGHVGDGN
                     LHYNAFPPPGDKRDFIAWSMEVNRIVYDVVQRLGGSISAEHGIGALKIDELPRYKSAL
                     ELDLMRAVKKALDPQGVMNPGKVLRP"
     gene            complement(4022..4501)
                     /locus_tag="VFV55_04440"
     CDS             complement(4022..4501)
                     /locus_tag="VFV55_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX4943578.1"
                     /translation="MVAPGFESKICEPRDFAARAKALARPLVFTNGVFDILHRGHVTY
                     LAEARALGEAMAIALNSDASARRLGKGDDRPINTLEDRLAVVAALESVCLVTWFDEDT
                     PIRRILECRPDRLVKGGDWPVERIVGATEVQGWGGSVHSIAFRHDRSTTRLLEKIRL"
BASE COUNT          722 a         1630 c         1701 g          746 t
ORIGIN      
        1 gaccaggtcg tggcgcgcgc acgcctcgta aacgaggctt tgcacggcga ggccacccac
       61 gttggagccc agcgcgcgcg tccccggcgg catggtcggc cagtctcggc ccagtctcgc
      121 ggccccggca gtggcgatcg cgccgcgcgc cacccactcc tcgcccacgc gcaaccccca
      181 cttcacgcgc gtgttgcccg cgtcgatggc gaggaccggc gccgtggcaa ggacgagggc
      241 gttgctcata gcgggcgaag cgagatctcg gcggaggtga ggcgcgcggt gcgcccgccg
      301 aaggcgacga cgagggcgcc gtcgtcggcg acgtcgagca cttgcgcctc ctgggccggt
      361 ttgtcgccgg cgtgcacggt tacgcgccgt ccggcataga cgtggcgcgc cttccagtcg
      421 tcgcggaagg cggcgaagcc ctggcggccg aagcgctcga gggcgatgac cagctcggcc
      481 agcaggccgg cgagcagggc gttgcgcgag ggcatcgccc cgccgcagga gaccacgtcg
      541 gtggcgggct ggtcgatgcg cccggcgagc gcctcgccca gccggtagtt gatcccaatg
      601 ccgatgatgg ccgccgaggg accgagcgtc tcgccggagg tctcgacgag aatgccgccc
      661 agcttcttcc agtcgtgcac gaggtcgttg ggccacttca cgccgacgcc cggcacgccg
      721 gcgcgctcga gcgcgcgcac caccgcgacc gccactgcga gcgaaagccc ggcgaggtgt
      781 cccggcccct gctcgaagcg ccacaggagc gaaaacgtga gcgaggcccc gagtcccgcc
      841 tgccagctgc gcccgcgacg cccgcggccg gcgcgctgcc actcggcggc cacgcacgtt
      901 cccgagggcg cgcccgcctg cgcagccgag gcgaggtgcg tgctcgtgga gtcgacctcg
      961 tcgagcagcg tgaggcgcac gcgcgaggcg acgggccaca ggcgcgagac gatggcgtgc
     1021 gcgtcgagga actcgatcgg ccgggcgaga cggtagccct tgcctggcac ggagaacacc
     1081 tcgacgccga gttcccgcgc ccccttgagc gactccgaca acgcggcgcg cgaacggtcg
     1141 agccggcgcg cgatctcggt gcccgagtgg aagtgcccgt cggccagcag ccgcaacgcg
     1201 ttgagggtga gcggccccat ctagccggcc cggtgctcgg cccggcactc gaaggcctcg
     1261 cgccggcagg cgatgtcggc gacgatcgca tcgagcagca gctccatccg tcgcgagagc
     1321 ttgctgccct tgcggtagtc ggcctgcgcg acgtaatccg cgcggcccgc ctcgaggagc
     1381 ttcacgcagg tggccttgcc gcgctcgtcg tccttcgcat acacggccag cgtgcgcccg
     1441 ccgttcttct tggtgagcgc catcgagggc acgtcggtca tcccgtcgcc cacgtagatc
     1501 atgttctgga acgggatggg tcgctcggct tcgggcatgt gctcgttgat cgactcggtg
     1561 accgcttcct tgcccttgtt gatgcggaag aggaactgcg tcttcaccgt gtccgtcacg
     1621 aggagcttgg gaaaagtggc cacgccgtgg tggttgaagt gatactcgga cgcgtagatg
     1681 cgcctgaagt gacggcggat ggagacgccg tccaggattt ccttctgccc cgcggagatc
     1741 acgtagtggc gcagcttcac ccggccgccg ctctgcttgc gcacgtagtc gttcacgcgc
     1801 cggaaccagc cttccacgcc ggggaagtag cggatggcgc tcgccatctt cgcgaagtcc
     1861 tcgcgcttca ccgcgatctt ctgccggtcc agggactcga ggatgtggcg catgtagacc
     1921 agcatcatgt ccgactcagt tgccagcgct tcctcgttca cgcgcttcca gaattcgcgc
     1981 ggcgcgatcc ccatgcgggg cagaaccgtg tactcctgca tcggttgcgg cgagagcgtg
     2041 ccgtcgaagt cgtagaccag cgcgatggtg ttctggaaga aactcttggc cacgggcacg
     2101 gtcggactcg ggatggcagg ggttcattct aaccaaagcc ccgccgccgc accctccgtc
     2161 agcagcggct gcattccggt tcgtcgcaag gggaggcctg gccatgggac cccggttcct
     2221 gccgtcgttg cgcaatcggc gccgcttcgc gcagcgaagt gaatgccgtg aatcggttat
     2281 ccccggcctt gcccgactct cacgaggacg gcggtgtgaa ctccgcgagc atgcgcgcga
     2341 gattgtcttc catcatcttg cggtactgcg cggcgaagag gtcgagcgcc tcctcggggg
     2401 actgcttgcc gggcgccccg ctcatggccc aggtgaaggc tccatagcgc gaggcggccc
     2461 acaggatcgc ggcggcagcg cggggccggc cgtgctcctc gccgatgtcg ttggcgagcg
     2521 cgacaaagcg gttggcgagg tcgaagaagg ctttcgcatc ctcgggaatc tgcgtggtca
     2581 cgggcggagc accttgccgg gattcatcac gccctgcggg tcgagcgctt tcttgacggc
     2641 gcgcatgaga tcgagctcga gcgccgactt gtagcgcggc aactcgtcga tcttgagggc
     2701 gccgatgccg tgctcggcgc tgatcgatcc gccgaggcgc tgcacgacgt cgtagacgat
     2761 gcggttcacc tccatcgacc aggcgatgaa gtcgcgcttg tcgccgggcg gggggaaggc
     2821 gttgtaatga aggttgccgt cgccgacatg gccgaaggcg acggtgcgga tgcccgggaa
     2881 tgcggcctgc agcgcgcggt cgccatcctc gatgagcttc ggcacgcacc ccacgggaac
     2941 ggacacgtcg tgcttcacgg agacgccctc gagtttctgc gcgtcggaga cgttttcccg
     3001 cagcgcccac aactcgaggc gctgcgcttc gctggcggcg acgacggcat cctgcacgag
     3061 ccccgcctcg gcggactcgc ccagcgcggt ctcgagcatc tccgccagtc cgctgcctgc
     3121 gctgctgtcg aaaagttcca ccagcacgta ccacgggtgc ccttgcggca gggggtcgcg
     3181 gctcgccgtg atgtgcctga agacgagatc gaagcaaacg cgcgcgatga gctcgaaccc
     3241 ggtgacgcgc tcgccgcacg cggcgcgcag cttcccgagc agggttaccg ccgcggccgg
     3301 atcggcaacg gccaccagcg cggtggcgat ggcgcgcggc atggggtaga gcttgagcac
     3361 cgccgcggtc acgatgccca gcgtgccttc cgccccgatg aacaggtgct tcaggtcgta
     3421 gcccgtgttg tccttgcgca gcccgcgcag cccgtcccac acgcggccgt cgggcagcac
     3481 cacttccagc cccagcacct gctcgcgcat gttcccgtag tgcagcacct gcgtgccgcc
     3541 ggcgttggtc gagatgtttc cgcccacctc gcaactgcct tcggcggcga gggacagggg
     3601 gaagaagcgg tcgttctgcg cggccacgcg ctgcaggtcg gccaggatgc agcccgcctc
     3661 gaccgtgatg gtgtcgttgt cgaggtcgac ggcgcgcacg cggttcatgc gcgagacgct
     3721 gacgatcacc tgggtgccgg aggcgtcggg gaccgaggcg ccgcacaggc tcgtgttgcc
     3781 gccctgcggg acgatgccca cgccggccgc ggagagcagc gcaacgacct tcgacagctc
     3841 ctgcgtcgag gccggtctca cgagaagcgc cgcgcggccc ctgaactggc cgcgccagtc
     3901 cgtgacgaac ggttccaggt cggccgcggc ggtcacgagt ccgcggtcgc ccacgatggc
     3961 gtgcagctcg ccggccagtg cggcggggaa gggagttcgg tcgttcatgg attcgcgggg
     4021 gctagagccg tatcttctcg aggaggcggg tggtcgagcg gtcgtgccgg aacgcgatcg
     4081 aatggacgct gccaccccag ccctgcactt ccgtcgcgcc gacgatccgc tccaccggcc
     4141 agtcgccacc cttcaccagc cggtcggggc ggcattcgag gatgcgccgg atcggcgtgt
     4201 cctcgtcgaa ccaggtgacg aggcagacgc tttccagcgc cgcgaccacg gcaaggcggt
     4261 cctcgagggt attgatcggc cggtcgtcgc ccttgcccag gcgccttgcg gacgcgtccg
     4321 agttgagcgc gatcgccatt gcctcgccca gcgcgcgcgc ctcggcaagg taggtcacgt
     4381 ggccgcggtg caggatgtcg aagacgccgt tggtgaacac gagcgggcgc gcgagcgcct
     4441 tcgcccgcgc ggcgaaatcg cgcggttcgc agatcttcga ttcgaagccg ggcgcgacca
     4501 tgacgacatt ctagtgcaag gtgacccgat ctccgtgcga gccctgcttc gaccatgacg
     4561 gatcgggtcc gcgtttcgcg gcggcggtgg cagtcgatca caatcgtggg caggtgaggt
     4621 gaggcggtgg cggtggaagt cgatcgcaat cctgggctcg tgggtcgagg aggcggcggc
     4681 ggtggaagtc gatcgcaatc ctgggcacgc aagcgagacc gtcggatggg tgcccggaaa
     4741 gtcgaggcca ggcagaacaa gccgggcctc gactttccac ccatctaatg acgagggcg
//
LOCUS       DASUEV010000135         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1626786, whole genome shotgun sequence.
ACCESSION   DASUEV010000135 DASUEV010000000
VERSION     DASUEV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626786"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFV55_04445"
     CDS             complement(<1..542)
                     /locus_tag="VFV55_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4943579.1"
                     /translation="MGARVNVGRVAVIGAGYAGIAAAVELARGGAAVTLFDANRTAGG
                     RARRVEYRATSLDNGQHVLLGAYRDTLDLMRTVSVPINALRRVPLALHYPGVLELAAP
                     PLPAPLHLLAALAAARGLSMRERYAAVRFAFAIRRAEAAANEGETVAAFLDRLAQPEP
                     LRRLVWEPLCVASLNTPADRA"
     gene            complement(527..1360)
                     /gene="hpnD"
                     /locus_tag="VFV55_04450"
     CDS             complement(527..1360)
                     /gene="hpnD"
                     /locus_tag="VFV55_04450"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX4943580.1"
                     /translation="MTPDEYCQQKAAASGSSFYYSFLFLPPDRRRAITAFYAFCREVD
                     DVADEASDVGVARAKLAWWRTEVANLMAGTASHPVTRALAPFVAPMGIDGRRMNEIID
                     GMEMDLTRHRYADFDSLRQYCHRVAGVVGQLSACIFGYRHAQTLEFAETLGLAFQLTN
                     IIRDVGEDARRGRVYLPEDELARHGLTVEDILARRGGEAFRAVMAEQAQRAESFYEQA
                     FARLHPEDRRDQRAGLIMAAIYRTLLSEIRRDGFPVLDGRVSLTPIRKLWIAWRTWVR
                     A"
     gene            complement(1357..2196)
                     /gene="hpnC"
                     /locus_tag="VFV55_04455"
     CDS             complement(1357..2196)
                     /gene="hpnC"
                     /locus_tag="VFV55_04455"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX4943581.1"
                     /translation="MPVEHYENFPVASWLLPPALRTPVEAIYAFARSADDFADEGERP
                     DAERLALLDGYARQLERIARRERPDDPVFSRLHDAIVTHDLPIPLFHDLLDAFRQDVV
                     KKRYATFDELMHYCRRSANPVGRLLLALFRKDDPANLRDSDAICSSLQLINHWQDIAV
                     DWRKGRLYLPQEDLVHFAVSEAQIAEGRVDGNWQNLVRFQCQKARAMMRSGSPLGTRL
                     PGRIGLEIRAISAGGLRILDKIDAVQGDVFRHRPVLAKGDWARILWKAAFRRPVPFRT
                     ARP"
     gene            2300..2384
                     /locus_tag="VFV55_04460"
     tRNA            2300..2384
                     /locus_tag="VFV55_04460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2334..2336,aa:Leu,seq:tag)
     gene            2458..3768
                     /gene="tig"
                     /locus_tag="VFV55_04465"
     CDS             2458..3768
                     /gene="tig"
                     /locus_tag="VFV55_04465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX4943582.1"
                     /translation="MQQAVETKTSPLERQLKLAVPVAQLEAEIAERIRKLARTVKMQG
                     FRPGKVPIKMVERHYGFQVRQEALSDTVQRQFADAVRSQNFRVAGLPRIEPVQAGADA
                     TAFEFQAVFEVYPEITMGELAERKVTRPVTEVSEKDVDATIETLRKQRATFERVERGA
                     AKGDLVNIDFEGKVDDVPFEGGSGKNFTVVLGEGRMLPDFEAALAGLVEGEEKTFPIV
                     FPEDYAEQLKGKTAQFTVKVNQVAQAKLPEVNAEFAKALGVEDGDLARMRTEIRQNVE
                     REVKKRIQSRVKEQVMQGLAAAATFEVPRALLEGEVARMREAALADLKQRGMTTQNIE
                     LPDDIFAGQARHRVTLGLLVADLVRRNNLAAQAAQVRKVIEEHADSFEQPAEMVRWFY
                     GQPERMAEVEAVVLEDNVVEWSLARMTVEDKPTAFEELMGTRKG"
     gene            3781..>4026
                     /locus_tag="VFV55_04470"
     CDS             3781..>4026
                     /locus_tag="VFV55_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX4943583.1"
                     /translation="MRDEYQGPQGLGLIPMVIEQSGRGERAYDIYSRLLKERVIFLVG
                     PINDSMANLIVAQLLFLESENPDKDINFYINSPGGSVS"
BASE COUNT          687 a         1292 c         1475 g          572 t
ORIGIN      
        1 gcccggtccg ccggcgtgtt gagcgaggcc acgcacaggg gttcccacac caggcgccgc
       61 agcggctcgg gctgcgcgag gcgatcgagg aacgcagcca ccgtttcgcc ctcgttggct
      121 gccgcctcgg cgcggcggat ggcgaacgcg aacctgaccg ccgcatagcg ctcgcgcatc
      181 gagagcccgc gcgcggcggc gagtgcggcg agaagatgca ggggtgcggg aagcggcggc
      241 gcggccagtt ccaggacacc ggggtagtgc agcgcgaggg gcacacgccg cagcgcattg
      301 atcgggacgc ttaccgtgcg catcaggtcg agcgtgtcgc gataggcgcc gagcaggaca
      361 tgttgcccgt tgtcgagcga cgtcgcgcgg tactcgacgc ggcgcgcgcg gccgccggcg
      421 gtgcgattgg catcgaagag ggtgaccgcg gcgccgcccc gcgccagttc caccgcagcc
      481 gcgatgccgg cgtagcccgc gccgatgacc gcgacccgcc ccacgttcac gcgcgcaccc
      541 atgtccgcca cgcgatccag agcttgcgga tcggggtgag ggagacacgc ccgtcgagca
      601 ccggaaagcc gtcgcggcgg atctcggaga ggagcgtgcg gtagatggcg gccatgatga
      661 gcccggcacg ctggtcgcgg cgatcctcgg gatggagcct ggcgaaagcc tgctcgtaga
      721 aggactcggc gcgctgcgcc tgctcggcca tcacggcgcg gaacgcctcg ccgccgcggc
      781 gggcgaggat gtcctcgacg gtgagcccgt ggcgcgcgag ctcgtcctcg ggaagataga
      841 cgcgcccgcg ccttgcgtcc tcgccgacgt cgcggatgat gttggtgagc tggaaggcga
      901 gacccagcgt ttcggcgaac tcgagcgtct gcgcgtggcg gtagccgaag atgcaggccg
      961 agagctggcc cacgacgccg gccacgcggt ggcagtactg gcgaagcgaa tcgaagtcgg
     1021 cgtagcgatg gcgcgtgagg tccatctcca tgccgtcgat gatctcgttc atgcgcctgc
     1081 cgtcgattcc catgggcgcc acgaagggcg ccagtgcgcg cgtcaccggg tgcgaggccg
     1141 tgcccgccat gaggttcgcg acttccgtcc gccaccacgc gagcttggcg cgcgcaacac
     1201 ccacgtcgct cgcctcgtcg gccacgtcgt ccacctcgcg gcagaacgcg tagaaggccg
     1261 tgatcgcgcg gcgcctgtcc ggcggcagga agaggaagct gtagtagaag ctcgagccgc
     1321 tggccgcggc cttctgctgg cagtattcgt cgggggtcat ggacgggccg tccgaaaggg
     1381 gacgggcctg cggaaggcgg ccttccacag gatgcgcgcc caatcgccct tcgccagcac
     1441 cgggcggtgg cggaagacgt cgccctgcac cgcgtcgatc ttgtcgagga tgcgcagccc
     1501 gcccgcggaa atggcgcgga tctcgaggcc gatgcgcccc ggcaggcgcg tgcccagggg
     1561 cgagcccgag cgcatcatgg cccgcgcctt ctggcactgg aagcgcacca ggttctgcca
     1621 gttgccgtcg acgcggccct cggcgatctg ggcttcgctc accgcgaaat ggacgaggtc
     1681 ctcctgcggc aggtagaggc gccccttgcg ccagtcgacg gcgatgtcct gccagtggtt
     1741 gatgagttgc aggctcgagc agatggcgtc ggaatcgcga aggttggccg gatcgtcctt
     1801 gcggaagagc gcgaggagca ggcggcccac ggggttcgcc gagcgccggc agtagtgcat
     1861 cagctcgtcg aaggtggcgt agcgcttttt caccacgtcc tggcggaagg cgtcgaggag
     1921 atcgtggaag agcgggatgg gcaggtcgtg ggtcacgatc gcgtcgtgca gccgcgagaa
     1981 caccgggtcg tcgggacgct cgcgccgcgc gatgcgctcg agctggcgcg catagccgtc
     2041 gaggagcgcc aggcgctcgg cgtccgggcg ctcgccttcg tcggcgaaat cgtccgcgct
     2101 cctcgcaaag gcgtagatgg cctcgaccgg ggtccgcagg gcgggcggca gaagccacga
     2161 tgcgacgggg aagttctcgt aatgctcgac cggcaagggg atgggaaacg cttgaattct
     2221 cgggattata tcggtccgcg gccgggccat gcgcggcgcc ggggattgcc ccctcttcgg
     2281 gtataatttc cccctcaccg cgaaagtggc ggaattggta gacgcaccgg atttaggttc
     2341 cggcgccgaa aggcatgggg gttcgagtcc cttctttcgc accaagttcg atcggccgat
     2401 accctgacgg ccgccccacg cggaacgcaa ccgggggaat cacacaggac tatccacatg
     2461 cagcaggcag tcgaaacgaa gaccagcccc ctcgagcggc aactgaagct ggcagtgccc
     2521 gtggcgcagc tggaagccga gatcgccgag cgcatccgca agctcgcgcg cacggtgaag
     2581 atgcagggtt tccggcccgg caaggtgccg atcaagatgg tggagcgcca ctacgggttc
     2641 caggtgcgcc aggaagcgct gtcggacacg gtgcagcgcc agttcgccga cgcggtccgc
     2701 agccagaact ttcgtgtcgc gggcctgccg cgcatcgagc cggtgcaggc cggggcggac
     2761 gcgacggcgt tcgagttcca ggcggtcttc gaggtctatc ccgagatcac catgggcgaa
     2821 ctggccgagc gcaaggtgac gcgcccggtg acggaggtgt cggaaaagga tgtcgacgcc
     2881 acaatcgaga cgctgcgcaa gcagcgcgcc accttcgagc gcgtcgagcg gggtgcggcc
     2941 aagggcgacc tcgtgaacat cgatttcgag ggcaaggtgg atgacgtgcc cttcgagggc
     3001 ggctcgggca agaacttcac cgtggtgctg ggcgaaggcc gcatgctccc ggacttcgag
     3061 gcggccctcg ccgggctcgt cgagggcgag gagaagacct tcccgatcgt gttccccgag
     3121 gactatgccg agcagctcaa gggcaagacc gcgcagttca ccgtcaaggt gaaccaggtc
     3181 gcgcaggcga agctcccgga ggtgaatgcc gagttcgcca aggcgctggg cgtggaggac
     3241 ggggacctcg cgcgcatgcg cacggagatc cgccagaacg tcgagcggga ggtgaagaag
     3301 cgcatccagt cccgggtgaa ggagcaggtg atgcagggac tcgccgccgc cgccacgttc
     3361 gaggtgccgc gcgcgctcct tgaaggggaa gtggcgcgca tgcgcgaggc cgccctcgcg
     3421 gacctcaagc agcgcggcat gaccacgcag aacatcgagc ttcccgacga catcttcgcc
     3481 ggccaggcca ggcaccgcgt gacgctgggc ctgctggttg ccgacctggt gcgcaggaac
     3541 aacctcgccg cccaggccgc ccaggtgcgc aaggtgatcg aggagcacgc cgacagcttc
     3601 gagcagcccg ccgagatggt gcgctggttc tacggccagc ccgagcggat ggccgaggtc
     3661 gaggcggtcg tgctggagga caacgtcgta gaatggtccc ttgcccgcat gacggtggag
     3721 gacaagccga cggccttcga agagctgatg ggcacccgca agggctgacg aggagacgcg
     3781 atgcgagacg aataccaggg gccgcagggc ctgggcctga tcccgatggt gatcgagcag
     3841 tcggggcgcg gagagcgcgc ctacgacatc tactcgcgcc tgctcaagga gcgcgtgatc
     3901 ttcctcgtgg gcccgatcaa cgactcgatg gccaacctga tcgtggcgca gctcctgttc
     3961 ctggagagcg agaaccccga caaggacatc aatttctaca tcaattcccc gggcggcagc
     4021 gtctcg
//
LOCUS       DASUEV010000136         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1529955, whole genome shotgun sequence.
ACCESSION   DASUEV010000136 DASUEV010000000
VERSION     DASUEV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529955"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFV55_04475"
     CDS             complement(<1..498)
                     /locus_tag="VFV55_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX4943584.1"
                     /translation="MATPEHPDAAFPASPVLPETFGPEREIRFQCRKGIGCWNACCSN
                     IDISLTPYDILRLKRRLDVSSGEFLREYTVPYEMEKDGIAGVKLRPVEGGTACRFMKP
                     EGCSVYEDRPTACRYYPVALLSMRRQGEATDRDSYALVKEPHCLGHDEPREITIADYR
                     NEQGLP"
     gene            complement(594..2660)
                     /gene="aprA"
                     /locus_tag="VFV55_04480"
     CDS             complement(594..2660)
                     /gene="aprA"
                     /locus_tag="VFV55_04480"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HEX4943585.1"
                     /translation="MEQGTFGNPNIVQEELDILLIGGGMACCGAGYEIMRWADAVKQE
                     TGVELRIKLIDKAAMDRSGAVAQGLSAINTYIGPDLDPADYARMVSNDLMGITRDDLA
                     YDLGRNVDDSVHLFEEWGLPIWKTDADGVRHDGAESIKEGLPLLKDGGKPVRSGKWQI
                     MINGESYKWIVAEAAKKALGLSRIQERTFIVKLVNDKNDPGRIAGAVGFSVRENTIYV
                     YKAKAILLAAGGCVNLFRPRSVGEGTGRAWYPVWNAGSTYAMAAEAGAEMTMMENRFV
                     PARFKDGYGPVGAWFLLFKAKATNAYGEDYMTKNKDLLNDYPPYGQAAVPASCLRNHL
                     MLKEMKEGRGPIWMDTVSALAKLRETLTPKEVKHLESEAWEDFLDMCIGQCGIWVGEN
                     IEPDKKNSELIPTEPYLLGSHSGCCGIWVSGPTDVGAPTAEEGDYDGVPAHLPRGWNW
                     GYRSMTTVKGLFTAGDGVGASGHKFSSGSHTEGRLAAKAMVKFALDNKDWKPELDTTP
                     EELAKQIYKPVMHFLEHKDYTTAIDINPHYITPKMLQFRLQKIMDEYVAGVATYYNTN
                     DKMLAIAEEKLEMLKEDAGKMRAKDLHELLRAWENYHRILTAEAHMKHIQFREESRYP
                     GFYYRTDKNFVDEENWHCFVNSIYDKSTRKWTCFKRKHVDLVDKSKLFKQAAAPAPAK
                     AAATAD"
     gene            complement(2705..>2961)
                     /locus_tag="VFV55_04485"
     CDS             complement(2705..>2961)
                     /locus_tag="VFV55_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HEX4943586.1"
                     /translation="DIVPLGGSVQPLRGSDSILWTIKFRNGTLKRFKFPIRTTAEGSI
                     DPYGGKPMPKVAELSAPGVFTKAMMGGYRAGRPDELIRCK"
BASE COUNT          429 a          958 c          994 g          580 t
ORIGIN      
        1 gggcagaccc tgctcgttgc ggtaatccgc gatggtgatc tcgcgcggct cgtcgtggcc
       61 caggcagtgc ggctccttca ccagggcata cgagtcgcgg tcggtcgcct cgccctgccg
      121 gcgcatcgac agcagcgcca ccggatagta gcggcaggcg gtgggccggt cctcgtagac
      181 gctgcaaccc tccggcttca tgaagcggca tgccgtgccc ccttccaccg gacgcagctt
      241 cacgccggcg atgccatcct tctccatctc gtaggggacg gtgtactccc tgaggaactc
      301 gcccgacgag acatccaggc gcctcttgag cctcaggatg tcgtagggcg tgagcgagat
      361 gtcgatgttg ctgcagcagg cgttccagca gccgatgccc ttgcggcact ggaagcggat
      421 ctcgcgctcg gggccgaagg tctcgggcag gacggggctc gcgggaaagg cggcgtcggg
      481 gtgttcggga gttgccatgc gggacctctg aagtgacacc gggcgcctcc ctggggaggc
      541 gcccggccat tacagcatgg aaaggcgcgg gacgtgcggc ccctgccttg cggtcagtct
      601 gcggtagcgg ccgccttggc cggcgccggc gccgccgcct gcttgaacag cttcgacttg
      661 tccacgaggt cgacgtgctt gcgcttgaag caggtccact tgcgcgtcga cttgtcgtag
      721 atcgagttca cgaagcagtg ccagttctcc tcgtcgacga agttcttgtc ggtgcggtag
      781 tagaagccgg ggtagcggct ctcctcgcgg aactggatgt gcttcatgtg cgcctccgcc
      841 gtgaggatgc ggtggtagtt ctcccaggcg cgcagcagct cgtggaggtc cttggcgcgc
      901 atcttgccgg cgtcctcctt gagcatctcg agcttctcct cggcgatggc gagcatcttg
      961 tcgttggtgt tgtagtacgt cgcgacgccg gccacgtact cgtccatgat cttctgcagg
     1021 cggaactgca gcatcttggg cgtgatgtag tgcgggttga tgtcgatcgc cgtggtgtag
     1081 tccttgtgct cgaggaagtg catcaccggc ttgtagatct gcttggcgag ttcctcgggc
     1141 gtcgtgtcca gctccggctt ccagtccttg ttgtcgagcg cgaacttgac catcgccttg
     1201 gcggccagcc gcccctcggt gtgcgagccc gaggagaact tgtgccccga ggcgcccacg
     1261 ccgtcgccgg cggtgaagag gcccttcacc gtggtcatcg agcggtagcc ccagttccag
     1321 ccgcgcggca gatgagccgg cacgccgtcg taatcgcctt cctcggccgt gggcgcgccc
     1381 acgtccgtgg gccccgacac ccagatgccg cagcagccgg agtgcgagcc gagcaggtag
     1441 ggttccgtgg ggatcagctc ggagttcttc ttgtcgggct cgatgttctc gccgacccag
     1501 atgccgcact ggccgatgca catgtcgagg aagtcctccc acgcctcgga ctcgaggtgc
     1561 ttcacctcct tgggcgtgag cgtctcgcga agcttggcga gggccgaaac ggtgtccatc
     1621 cagatcgggc cgcggccttc cttcatctcc ttgagcatca ggtgattgcg caggcaggag
     1681 gcgggaacgg ccgcctgccc gtagggcggg tagtcgttca gcaggtcctt gttcttcgtc
     1741 atgtagtcct cgccataggc attggtcgcc ttggccttga agagcaggaa ccatgcgccc
     1801 accggcccgt agccgtcctt gaatcgcgcc ggcacgaaac ggttctccat catcgtcatt
     1861 tcggcgccgg cttccgccgc catcgcgtag gtgctgcccg cgttccacac cgggtaccag
     1921 gcgcggcccg tgccctcgcc aaccgagcgc ggacggaaga ggttcacgca gccgcccgcg
     1981 gccagcagga tcgccttcgc cttgtagacg tagatcgtgt tctcgcgcac cgagaagccg
     2041 acggcgcccg caatgcggcc cgggtcgttc ttgtcgttca ccagcttcac gatgaacgtg
     2101 cgctcctgga tgcgcgagag ccccagggcc ttcttggcgg cctcggcgac gatccacttg
     2161 taggactcgc cgttgatcat gatctgccac ttgccggaac gaacgggctt gccgccgtcc
     2221 ttgagcagcg gcagcccctc cttgatggac tcggcgccgt cgtggcgaac gccgtcggcg
     2281 tccgtcttcc agatgggcag gccccactcc tcgaagaggt gcacggagtc gtcgacgttg
     2341 cggccgaggt cgtaggccag gtcgtcacgc gtgatgccca tgaggtcgtt ggagaccatg
     2401 cgcgcgtaat cggccggatc caggtccggg ccgatgtagg tgttgatggc cgacaggccc
     2461 tgcgccacgg cgccggagcg atccatggcg gccttgtcga tcagcttgat cctgagctcc
     2521 acgccggttt cctgcttgac cgcgtcggcc cagcgcatga tctcgtagcc ggcgccgcag
     2581 caggccatgc cgccgccgat gaggaggatg tccagctctt cctggacaat gttgggattg
     2641 ccgaaggttc cttgttccat tgccttgtcc tgtctcgagt cgattggcgg gaatggtgcg
     2701 tcgcctactt gcagcggatc agttcgtcgg gacggccggc gcgatagccg cccatcatcg
     2761 ccttcgtgaa cacgccgggc gccgaaagct cggccacctt gggcatgggc ttgccgccgt
     2821 acgggtcgat cgagccctcg gcggtggtgc ggatggggaa cttgaagcgc ttcagcgtgc
     2881 cgttgcggaa cttgatcgtc cacaggatgg aatcgctgcc gcgcagcggc tgcaccgagc
     2941 cgcccagcgg cacgatgtcc g
//
LOCUS       DASUEV010000137         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1627007, whole genome shotgun sequence.
ACCESSION   DASUEV010000137 DASUEV010000000
VERSION     DASUEV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627007"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFV55_04490"
     CDS             complement(<1..411)
                     /locus_tag="VFV55_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX4943587.1"
                     /translation="MPAASSPATVPAKSGIYASVLAQAKALFEGERDATANAANLAAL
                     LYHTLPELNWAGFYWLKDGGLVLGPFQGKPACVRIALGKGVCGTAAATRRTVVVPDVH
                     AFPGHIACDSASESEMVIPVMKAGRAIGVLDLDSP"
     gene            504..>2537
                     /locus_tag="VFV55_04495"
     CDS             504..>2537
                     /locus_tag="VFV55_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4943588.1"
                     /translation="MNAAAVLLAMVLTPAISFASPPLPPVATKDPVVMTKFGDRRVDD
                     YFWLRDKAGAGVIDYLKAENSYTEAVMQPLEGFRGDLYKEMLARIKETDESVPYRRGG
                     WWYYTREVEGLQYPIYCRRRGSMQAAEEVMLDVNELAAGKAYTGVDFWEVSPDGNYLA
                     YGADFTGYREYTVYVKDLRSGELLPDRLERVDDVAWAGDSATLFYVQQNAAKRPDRLF
                     RHKLGAPRDALVWEEKDELFNLSVGATRSEEWIVATSSSKDTSEVRVLPAVQPEALLR
                     MVAKRRKGHEYYVDHHGSEFWIRTNDKGRNFRLVRAPVTDPAPGNWRQVLVHRKLVML
                     EEVDLFKDFWVAKERADGVLKLRVTDFATGEFHYIDMPEPVYSTGAGTNAEYDTGTFR
                     VVYQSYITPRTVFDYDVGKRSRELLKQQPVLGGYEPGQYASEMLMATATDGTKVPVSL
                     VYRKSLRKGAPQPLLLYGYGSYGYPLDVNFRSSRLSLLDRGMVFAIAHVRGGGDRGRL
                     WYDDGKLRRKMNTFTDFIAAAEHLVATGWTAPDRLVIQGGSAGGLLMGAVANLRPDLF
                     KAVVSQVPFVDVVNTMLDATLPLTTQEYIEWGNPNKEGDYKYIRKYSPYDNIERKAYP
                     AMLVEASLNDSQVPYWEAAKYVAKLRACRTDSNVLLLKTILEAGHGGASGRYDA"
BASE COUNT          445 a          847 c          850 g          395 t
ORIGIN      
        1 cgggctgtcc aggtccagca cgcctattgc gcgaccggcc ttcatgacgg ggatcaccat
       61 ctcgctttcc gaggcgctgt cgcacgcgat gtggccggga aaggcgtgca cgtcgggaac
      121 gaccaccgtg cgccgcgtcg cggcggcggt gccgcacacg ccctttccca gcgcgatgcg
      181 cacgcaggcg ggcttgccct ggaagggtcc cagcaccagc ccgccgtcct tcagccagta
      241 gaaaccggcc cagttgagct ccggcagcgt gtggtagagg agggcggcga gattggcggc
      301 gttggccgtc gcgtcccgct cgccttcgaa gagcgccttt gcctgcgcga gcacgctcgc
      361 gtagatcccg gacttggcgg ggacggttgc ggggctggat gcggcgggca tggcggcgat
      421 cacggggaag gctgctaggt taccacccgt cccgcttcgc tataattcgc gctctcctgc
      481 cctggggctc tcccccgact gccatgaacg ccgccgcagt cctcctcgcc atggtcctca
      541 cccccgccat ttccttcgca agccctccgc tcccgcccgt cgccacgaag gatccggtgg
      601 tgatgacgaa attcggcgac aggcgtgtcg atgactactt ctggctgcgc gacaaggccg
      661 gtgccggggt catcgactac ctgaaggccg agaactccta caccgaggcg gtcatgcaac
      721 ccctcgaagg cttccgcggc gacctctaca aggaaatgct tgcccgcatc aaggaaaccg
      781 acgagagcgt cccgtaccgg cgcggcggat ggtggtacta cacgcgcgag gtcgagggcc
      841 tgcagtaccc gatctattgc cgccgcaggg gctcgatgca ggccgccgag gaagtgatgc
      901 tggacgtgaa cgaactggcc gcgggcaagg cctacacggg cgtcgatttc tgggaggtga
      961 gccccgacgg caactacctg gcctacggcg ccgatttcac cggctaccgc gagtacacgg
     1021 tctacgtgaa ggacctgcgg tcgggcgagc tgcttccaga tcgcctcgag cgcgtcgacg
     1081 acgtggcctg ggcaggtgac agcgccacgc tcttctatgt ccagcagaac gcggccaagc
     1141 ggccggaccg gctcttccgc cacaagctgg gcgcaccccg ggacgcgctg gtctgggagg
     1201 agaaggacga gctcttcaac ctgagcgtcg gcgccacgcg cagcgaagag tggatcgtgg
     1261 caacgtcatc gtccaaggac acttcggaag tgcgcgtgct gcctgcggtg cagcccgagg
     1321 cattgctgcg catggtggcg aagcgccgca agggacacga atactacgtg gaccaccacg
     1381 gcagcgagtt ctggatccgc accaacgaca aggggaggaa cttccggctg gtgcgggcgc
     1441 ccgtcaccga tcccgccccg ggcaactggc ggcaggtgct cgtgcatcgc aagctcgtga
     1501 tgctcgagga ggtcgacctg ttcaaggatt tctgggtggc gaaggagcgc gccgacgggg
     1561 tgctgaagct gcgcgtcacc gacttcgcga cgggcgaatt ccactacatc gacatgcccg
     1621 agcccgttta ctcgaccggg gccggcacca acgcggaata cgacaccggc accttccgcg
     1681 tcgtctacca gagctacatc acgccgcgca cggtgttcga ctacgacgtg ggcaagcgca
     1741 gccgcgagct cctcaagcag cagccggtgc tgggcggcta cgaacccggg cagtacgcga
     1801 gcgagatgct catggccacc gcgacggacg gcacgaaggt cccggtgtcg ctcgtgtacc
     1861 ggaagtccct gcgcaagggc gccccgcagc cgctgctgct ctacggctac ggctcctatg
     1921 gctatcccct cgacgtgaac ttccgctcct cgcggctttc actgctggat cgcggcatgg
     1981 tgttcgccat cgcgcacgtc cgcggcgggg gcgaccgggg caggctctgg tacgacgacg
     2041 gcaagctgcg ccggaagatg aacaccttca ccgacttcat cgcagccgcg gagcacctgg
     2101 tcgcgacggg gtggacggcg cctgaccggc tggtgatcca gggcggcagc gccggcgggc
     2161 tgctgatggg cgcagtggcc aacctcaggc cggacctgtt caaggccgtg gtgagccagg
     2221 tgcccttcgt ggacgtggtg aacacgatgc tcgacgccac gctccctctc acgacgcagg
     2281 aatacatcga gtggggcaac ccgaacaagg aaggggacta caagtacatc cgcaagtact
     2341 ccccctacga caacatcgag cgcaaggcct atcccgcgat gctcgtcgaa gcgtctctga
     2401 acgacagcca ggttccctac tgggaggcgg ccaagtacgt ggcgaagctg cgcgcctgca
     2461 ggacggattc gaacgtgttg ctcctcaaga cgatcctcga ggccggccac ggcggggcct
     2521 cgggccgcta cgacgcg
//
LOCUS       DASUEV010000138         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_958052, whole genome shotgun sequence.
ACCESSION   DASUEV010000138 DASUEV010000000
VERSION     DASUEV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958052"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1292)
                     /gene="recA"
                     /locus_tag="VFV55_04500"
     CDS             complement(252..1292)
                     /gene="recA"
                     /locus_tag="VFV55_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX4943589.1"
                     /translation="MDENKAKALQAALTQIERQFGKGAIMRMGDKDVEKGIEVVSTGS
                     LGLDLALGVGGLPRGRVVEIYGPESSGKTTLTLQVIANIQKLGGTAAFIDAEHALDPQ
                     YAQRLGVVTSELLLSQPDNGEQALEIADMLVRSASVDCVVVDSVAALTPKAEIEGEMG
                     EPQMGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRIGSIKRGEEVIGSETRVKVVKNKVAPPFKVADFDILYNEGISRHGEIIE
                     MGVQHRIIDKSGAWYAYKGEKIGQGKDNTREFLKENPAMSDEIEAKIREAVGIAGGMP
                     LDNGAAEAAEAD"
     gene            complement(1378..1974)
                     /locus_tag="VFV55_04505"
     CDS             complement(1378..1974)
                     /locus_tag="VFV55_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX4943590.1"
                     /translation="MALKNHQPLDPAYLEKLQDYYAEHKVIPSYSVLATLWGISAKSW
                     VSECVRRFEEAGYLDWTPDRQLKPGSRFFERRLAHDTVQAGLPNPALADGYDLVTIDD
                     YLIRVPSKTSLVRVKGESMIDAGIHDGDLIVVEQQPNANVGEIVVAIVDNEFTVKYLD
                     REKGAFVLKPANKAFPVIRPRGRLEIFGVMAGLVRKTR"
BASE COUNT          384 a          720 c          753 g          458 t
ORIGIN      
        1 gatttggcgc gcaccgtctg ctcgacgaag cgctcttccg aaagccaccc gcgcttcgtg
       61 aaatcatcca ggagagcctc gatgtcgaag ccctcctgca catgcggcgc cagcttgcgg
      121 cgcagttcag cccgggaatg ctcgcggcgg gcgagcagcc tcaatgcgcg cgcacgaact
      181 tccgcatcca tcgcagggac ggccagaccc cggggcatgg gcagacagcg gcgttagcga
      241 ccgccgaagg gtcagtccgc ctcggccgcc tcggccgcgc cgttgtcgag gggcatgccg
      301 cccgcgatgc cgaccgcctc ccggatcttc gcctcgatct cgtcggacat cgcggggttt
      361 tccttgagga attcgcgcgt gttgtccttg ccctggccga tcttctcgcc cttgtaggcg
      421 taccaggcgc cgctcttgtc gatgatgcgg tgctgcacgc ccatctcgat gatctcgccg
      481 tggcgcgaga tgccttcgtt gtagaggatg tcgaagtccg cgaccttgaa ggggggcgcg
      541 accttgttct tcaccacctt cacgcgcgtt tcgctgccga tgacttcctc tccgcgcttg
      601 atggagccga tgcggcggat gtccaagcgg acggaggcgt agaacttgag cgcgttgccg
      661 ccggtagtgg tctcggggtt gccgaacatg acgccgatct tcatgcggat ctggttgatg
      721 aagatgacca gcgtgttcga gcgcttgatg ttggcggtga gcttgcgcag cgcctgcgac
      781 atgaggcgcg cctgcaggcc catctgcggc tcgcccatct cgccctcgat ctcggccttg
      841 ggcgtcagcg ccgcgacgga gtcgacgacc acgcagtcga ccgaggccga acgcaccagc
      901 atgtcggcga tctcgagcgc ctgctcgccg ttgtccggct gcgagagcag gagctcggag
      961 gtgaccacgc cgaggcgctg ggcgtactgg ggatcgagcg cgtgctcggc gtcgatgaag
     1021 gcggcggtgc cgcccagctt ctggatgttg gcgatgacct gcagcgtgag cgtggtcttg
     1081 ccggacgatt ccgggccgta gatctcgacg acgcggccgc gcggaaggcc gccgacgccg
     1141 agggcgagat cgagccccag cgacccggtg gagacgacct cgatgccctt ctcgacatcc
     1201 ttgtcgccca tgcgcatgat cgcgcccttg ccgaactggc gctcgatctg ggtgagcgcg
     1261 gcctgcaggg ccttggcctt gttctcgtcc atcttggggg tgtcggaagc gctggtggag
     1321 atgactttga gcattggaat tatcccatat cggtgaagtt gaattttccg cccgggccta
     1381 gcgcgtcttg cgcaccaggc ccgccatcac accgaaaatt tccaggcgcc cgcgggggcg
     1441 aatgaccggg aaggctttgt tcgcgggctt caggacgaag gcgccttttt ctcggtccag
     1501 gtacttcacg gtgaattcgt tgtccacgat cgccacgacg atctcgccga cgttggcgtt
     1561 cggctgctgc tcgaccacga tgaggtcgcc gtcgtggatg ccggcgtcga tcatcgattc
     1621 gcccttcacg cgcaccaggc tcgtcttcga cggcacccgg atcaggtagt catcgatggt
     1681 gacgaggtcg tagccgtcgg caagcgccgg gttgggcagc cccgcctgca cggtatcgtg
     1741 cgcgagccgc cgttcgaaga accgggagcc gggcttgagc tgccggtcag gagtccagtc
     1801 caggtagccc gcttcctcga aacggcgcac gcactccgac acccacgatt tggccgagat
     1861 cccccacagg gtggcgagca ccgagtagga ggggatcacc ttgtgctcgg cgtagtagtc
     1921 ctggagcttc tccaggtacg ccgggtcgag gggctggtgg ttcttcaacg ccatgggcgg
     1981 gcaaccggtt tacagaacga ttgttcggta agttacagaa cgatcgttct gatgtcaagc
     2041 ggtgaaaagg ggtcaggtta ctttgcacaa agaataggca aaacggaaaa ggggtcaggt
     2101 tacttttctg tcgtattgcc gtcgcaagac ggcgggctga aaagtaacct gacccctttt
     2161 ccaaagtaac ctgacccctt ttcctcacct gacccctttt ccttcaggaa ttgagcagga
     2221 tcttcaggtc ggacgcgatc ttgtctcccg gcgtcccgta aggaagcatg aggcgcaccc
     2281 gcccctgggt atcgaacacc agggtttgcg ccgaa
//
LOCUS       DASUEV010000139        12336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1166093, whole genome shotgun sequence.
ACCESSION   DASUEV010000139 DASUEV010000000
VERSION     DASUEV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12336
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166093"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1857
                     /gene="ftsH"
                     /locus_tag="VFV55_04510"
     CDS             7..1857
                     /gene="ftsH"
                     /locus_tag="VFV55_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX4943591.1"
                     /translation="MVFMQVGNRQTSANVLDYSTFMEESRQGRIAKVKMDGNRTLKAT
                     SKDGKAYTVYTPGIMDPWMVSDLLKNGVTVEAKPEEEPSLLMNIFVSWFPMLLLIGVW
                     IFFMRQMQGGGRGGAFSFGKSRARMLDENQNQVTFADVAGVEEAKEEVSELVEFLRDP
                     SKFQKLGGRIPRGVLMVGSPGTGKTLLARAIAGEAKVPFFSISGSDFVEMFVGVGAAR
                     VRDMFEQAKKNAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFEGNSG
                     VIVIAATNRPDVLDPALMRPGRFDRQVVVPLPDIRGREQILMVHMRKVPIAPDVDASV
                     IARGCPGFSGADLANLVNEAALFAARSTKRLVDMEDFERAKDKILMGAERRSMVMPEE
                     ERRNTAYHESGHAIVAKLLPRTDPVHKVTIIPRGRALGVTVQLPEQDRYSYDRSLLLN
                     NIAVLFGGRIAEELFMNQMTTGASNDFKRATEMARRMVTEWGMSDSLGPMVYSENEGE
                     VFLGRSVTTHQAISEATMQHVDKEIKRIIDEQYNLARKLLEDNRDKVEAMTKALLEWE
                     TIDAEQINDIMAGMPPRPPKPVATKPNVPPPEGGAKGAEPPAQPSPAESA"
     gene            1908..2744
                     /gene="folP"
                     /locus_tag="VFV55_04515"
     CDS             1908..2744
                     /gene="folP"
                     /locus_tag="VFV55_04515"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX4943592.1"
                     /translation="MTPTLRCGDFTLVLDRPRVMGILNVTADSFSDGGRFLAEARAIE
                     HGLALAADGADLVDVGGESTRPGAEPVPVEMELARVMPVVRALVRGGVKVSVDTMKPE
                     VMRQAIDAGCAMVNDVNAFRAEGAAAIVARSGVGLCAMHMRGTPATMQADPQYDDVMA
                     EVRAYLLERAGALEAAGVSRDRIAIDPGFGFGKNFAHNVALLAGLPALAATGYPVVAG
                     LSRKRMIGEITGRPVDQRVAGSVAAALLAVQNGAGIVRVHDVKETVDALQIWRALDGV
                     RP"
     gene            2741..4114
                     /gene="glmM"
                     /locus_tag="VFV55_04520"
     CDS             2741..4114
                     /gene="glmM"
                     /locus_tag="VFV55_04520"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX4943593.1"
                     /translation="MKSRQFFGTDGIRGRVGDPPITPDFVLRLGYAAGLVLSRADVAP
                     PHRDRPAVLIGKDTRISGYMLESALEAGLSAAGVDTLLVGPMPTPAVAFLTRALRLSA
                     GIMVSASHNPFEDNGIKFFSAEGAKLPDAVEHDIEAVLREPLVTRDSAHLGKAERVHD
                     AEGRYIEFCKGTFAKHRTLRALKLVVDCAHGACHRLGPRVFQELGAEVVAIGVHPNGT
                     NINHGCGATHPATLARAVIDHDADFGIAFDGDGDRLAMADHDGRLFDGDQLLYAIAKH
                     RHETGRLLGGVAGTLMTNFALERRLGELGIPFARAKVGDRYVLELLEARGWECGGENS
                     GHILCLDCHSTGDAIISALQVLEALVAAGQTLGEYTRELALLPQVLVNVRLEPGVDAM
                     NDARVREAVASAESQLAGRGRVLLRPSGTEPLIRVMVEGEDPARVRALADSIAATVRG
                     IAQPVAG"
     gene            4137..5705
                     /gene="gshA"
                     /locus_tag="VFV55_04525"
     CDS             4137..5705
                     /gene="gshA"
                     /locus_tag="VFV55_04525"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX4943594.1"
                     /translation="MRKLPPEVLRGLLRGIEKEGLRVRADGMLSSAPHPEGLGAALTH
                     PHITTDFSESQLELITGVHADVDDCLRELTELHQVVYRQLGNELLWCASMPCKLPPDD
                     LIPIARYGSSNVARLKTVYRHGLAHRYGRRMQVISGIHYNFSLPESAWAHLHGADGGA
                     VPADLFRDQRYLALIRNFRRHSWLLLLLLGTSPAACGSFVAGRNHGLAAWETGSVFAP
                     GATSLRMGRLGYQSDAQSSLAVSFNDLGGYAKALGRALTEPYPAYEAIGLREGDDYLQ
                     LSTSLLQLENEFYGTIRPKRRTLPDERPLRALGERGIEYVEVRCLDVSPFSPVGIDAD
                     DMRLLDIFLLHCLLGDSPPDSPREIEAISRNQRLVAEKGRDPLVRLDRAGVRIAPADW
                     GADLLLQFEPIAAALDEAHGGGKYRGVLADARRALGDLSSLPSARVLRETQAEHGTSF
                     PDFALTQSLVHQETLAALPLAAEVEARYAKVAQQSLAEQRRIEAGDDVPFETYRQGYL
                     GQELLAGPHFRPGH"
     gene            complement(5737..6009)
                     /locus_tag="VFV55_04530"
     CDS             complement(5737..6009)
                     /locus_tag="VFV55_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX4943595.1"
                     /translation="MAITEFQALLEMQCQAVLAQLQKAGPNHMPRLDNERRKVEAAID
                     RLEAGTYGICCRCEEEVTPERLRADPATPFCQECIDEPAPAMARAW"
     gene            6291..6665
                     /locus_tag="VFV55_04535"
     CDS             6291..6665
                     /locus_tag="VFV55_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX4943596.1"
                     /translation="METERATEILECLSSPTRLRIYRLVVRQGAEGMVAGQIATQLDI
                     PPNGASFHLKAMTHARLLSVKQEGRFQRYRADITQMFDLVAYLTEECCAGHPELCAPA
                     AVVRVPVPGLKAPASRAPRAKR"
     gene            6662..7141
                     /locus_tag="VFV55_04540"
     CDS             6662..7141
                     /locus_tag="VFV55_04540"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX4943597.1"
                     /translation="MNVLFLCTGNSCRSILAEAAFNHLAPGGWRAMSAGSHPTGTVHP
                     RSLALLAREGVSTEGCRSKSWDDLLAVPDIVVTVCSSAAGETCPAYLGPVLRTHWGVD
                     DPAHATGTDEEIDAAFMKAYRILRARIEAFFALPLESLKNDKASLKAGMDRIANITS"
     gene            7153..7515
                     /gene="arsD"
                     /locus_tag="VFV55_04545"
     CDS             7153..7515
                     /gene="arsD"
                     /locus_tag="VFV55_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HEX4943598.1"
                     /translation="MVTIQVFEPALCCSSGVCGADVDPQLVAFSADADWLAKQGGRIE
                     RLNLAQQPLAFAQNAVVKEFLERSGQEALPLVLVDAKVALAGRYPRRAELARWSGIGA
                     PVAQAEAAGGCCSGGRCC"
     gene            7542..9335
                     /gene="arsA"
                     /locus_tag="VFV55_04550"
     CDS             7542..9335
                     /gene="arsA"
                     /locus_tag="VFV55_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HEX4943599.1"
                     /translation="MLFLENVPPFLFFTGKGGVGKTSLACATAVRLVDGGKRVLLVST
                     DPASNVGQVFGVTIGNAITAIDEVPGLSALEIDPQAVAQQYRDRIVGPVRGVLPDAVV
                     KGIEEQLSGACTTEIAAFDEFTALLTDRALMGKYDHIVFDTAPTGHTIRLLQLPGAWT
                     GFLDAGKGDASCLGPLAGLEKQRTQYQDAVQALADPERTRLVLVARAQDSALGEAART
                     HGELASIGLGRQHLVINGVLAEGDVAGDPIAAAIFRRERGAMDAMPGELRGLPVDRVP
                     LKGFNLVGVPALRRFFADAAAAAATPAPLSLPLAAELAGLVDDIAREGHGLVMLMGKG
                     GVGKTTLAAAVAIELAGRGHPVHLTTSDPAAHLAETLEGTLEHLVVSRIDPRAETQRY
                     RAHVLEVKGRNLDEHGRALLEEDLRSPCTEEIAVFQAFSRIIQEAGTKFVVMDTAPTG
                     HTLLLLDATGAYHREVARQFGPGGTHFTTPMMRLQDGRRTKVLIVTLAETTPVLEAAE
                     LQADLRRAGIEPWAWIVNNSLAFARPVSPLLRMRAANEIAQIEAVAHRHARRWAVVPV
                     QPEEPVGIERLRNLARSAQPATEEVCDVRAM"
     gene            9319..10395
                     /gene="arsB"
                     /locus_tag="VFV55_04555"
     CDS             9319..10395
                     /gene="arsB"
                     /locus_tag="VFV55_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX4943600.1"
                     /translation="MSAQCEVTAKRVSGAPLGFFERYLTAWVALCIVVGIILGQFLPA
                     VFQAIGAMEVAKVNLPVGVLIWVMIIPMLLKIDFGALHQVKGHIRGIGVTLAINWLVK
                     PFSMAFLAWLFIRHLFAPLLPAGQIDSYIAGLILLAAAPCTAMVFVWSRLTNGDPYFT
                     LSQVALNDVIMIFAFAPVVGFLLGVASITVPWDTLFTSVVLYIVIPVIIAQVIRRQLL
                     ARGQEAFDAAMAKIQPWSVSALLLTLVLLFAFQGNAILKQPLVIALLAVPILIQVFFN
                     SSLAYLLNRAVGEKHSVACPSALIGASNFFELAVAAAISLFGFESGAALATVVGVLIE
                     VPVMLLVVRVVNATKPWYERGARA"
     gene            10392..10976
                     /locus_tag="VFV55_04560"
     CDS             10392..10976
                     /locus_tag="VFV55_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4943601.1"
                     /translation="MNCLMRAWRQHEGELRGWLRRRLGNPVDAEDALQELFLKALRQR
                     ERFCEIINARAWLFEVARNALADRLRLSRDMVELPEDLAAAAEETAPVDSLAGCLPRV
                     LSELSPGDREAITLCDLGGMPQEEYARLKGLSLPGAKSRVQRARKRLRQQLMTGCRVS
                     LDAVGQVSDFVPRPPIAGATRRVRRGARRDCDTS"
     gene            11077..11346
                     /locus_tag="VFV55_04565"
     CDS             11077..11346
                     /locus_tag="VFV55_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX4943602.1"
                     /translation="MQTRTLTIANPGGLHAQACARIVAIAKRFRCNLSIVARGQRVRA
                     RNIFAVMLITASVGATVRIEADGPDEDMAMREIATLFHDGLGERT"
     gene            complement(11353..12066)
                     /locus_tag="VFV55_04570"
     CDS             complement(11353..12066)
                     /locus_tag="VFV55_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943603.1"
                     /translation="MSADLEELDYRSTPIGELVLRRRREPALGIDLFEVKLGDDFLMS
                     SLFTAGEIALAERGLHGLGDGPLDVVVGGLGLGYTARAVLDHAKVGSLLVVEALPAVI
                     DWHRRGLVPLGAGLASDLRCEFLNGDFFALVDTPGIDSRDSRRRFDAILVDIDHSPRH
                     VLNASHAAFYRRDGLRRLAAHLRPGGSFALWSDDPPDAEFISELEAVFAAAVAHVVEF
                     ANPLAGGTASNTVYVARTR"
BASE COUNT         1921 a         4214 c         4204 g         1997 t
ORIGIN      
        1 ctgaccatgg tcttcatgca ggtcggcaat cgccagacca gcgccaacgt gctcgactac
       61 tcgaccttca tggaggagtc gcggcagggc cgcatcgcca aggtgaagat ggacggcaac
      121 cggacgctca aggccacctc caaggacggc aaggcgtaca cggtctacac gccgggcatc
      181 atggacccgt ggatggtctc cgacctcctc aagaacggcg tcacggtcga ggcgaagccc
      241 gaggaggagc cctccctcct catgaacatc ttcgtttcgt ggttcccgat gctgctcctg
      301 atcggcgtgt ggatcttctt catgcgccag atgcagggcg gcgggcgcgg cggcgcattc
      361 tccttcggca agtcgcgcgc gcgcatgctt gacgagaacc agaaccaggt cacgttcgcc
      421 gacgtcgcgg gcgtcgaaga ggccaaggag gaggtcagcg agctggtcga attcctgcgc
      481 gacccgtcca agttccagaa gctgggtggc cgcatccctc gtggcgtgct gatggtgggc
      541 tcgcccggca cgggcaagac gcttctcgcg cgcgccatcg cgggcgaggc caaggtgccg
      601 ttcttctcca tctccggctc cgacttcgtc gagatgttcg tgggcgtggg cgctgcccgc
      661 gtgcgcgaca tgttcgagca ggccaagaag aacgcgccgt gcatcgtgtt catcgacgag
      721 atcgacgccg tgggccgcca gcgtggcgcg ggcctgggcg gcggcaacga cgagcgcgag
      781 cagacgctca accagcttct cgtcgagatg gacggcttcg agggcaactc gggcgtcatc
      841 gtcatcgccg cgaccaaccg gccggacgtg ctcgaccctg ccctcatgcg cccgggccgc
      901 ttcgaccgcc aggtggtcgt gccgctgccc gacatccgtg ggcgcgagca gatcctgatg
      961 gtgcacatgc gcaaggtgcc catcgcgccg gacgtggacg cctccgtcat cgcgcgcggc
     1021 tgcccgggct tttccggcgc ggacctcgcc aaccttgtga acgaggcggc gctcttcgcc
     1081 gcgcgctcga ccaagcgcct ggtggacatg gaggacttcg agcgcgccaa ggacaagatc
     1141 ctgatgggcg ccgagcggcg ttccatggtc atgcccgagg aagagcgccg gaatacggcg
     1201 taccacgagt cgggccacgc gatcgtcgcg aagctcctgc cccgtacgga cccggtgcac
     1261 aaggtgacca tcatcccgcg cggccgcgcg ctgggcgtca cggtgcagtt gccggagcag
     1321 gaccgctaca gctacgaccg gagcctgctg ctcaacaaca tcgcggtgct cttcggcggc
     1381 cggatcgccg aggagctctt catgaaccag atgaccaccg gcgcctcgaa cgacttcaag
     1441 cgcgccaccg agatggcccg ccgcatggtg acggagtggg gcatgtccga cagcctcggg
     1501 cccatggtct acagcgagaa cgagggcgaa gtcttcctcg gccgctcggt gaccacgcac
     1561 caggcgatat ccgaggcgac catgcagcac gtcgacaagg agatcaagcg catcatcgac
     1621 gagcagtaca acctcgcccg gaaactcctc gaggacaacc gcgacaaggt cgaggcgatg
     1681 accaaggcgc tgctcgagtg ggaaacgatc gacgcggagc agatcaacga catcatggcc
     1741 ggcatgccgc cgcgcccgcc caagccggtg gcgacgaagc ccaacgtgcc cccgccggaa
     1801 ggcggcgcga agggcgcgga accgccagcg caaccgtcgc cggcggaaag cgcctgacgg
     1861 gcgtaatgaa cggacaggcg gggcggcttc ggccgccccg ttttgctatg acccccaccc
     1921 ttcgctgcgg cgacttcacc ctcgtgctcg accggccgag ggtgatggga attctcaacg
     1981 tcaccgccga ctccttctcc gacggcggcc gattcctcgc cgaggcaagg gccatcgagc
     2041 atggcctggc gctcgccgcc gacggcgccg atctcgtgga cgtgggtggc gaatcgacgc
     2101 gcccgggagc cgagccggtg cccgtcgaaa tggagcttgc gcgcgtgatg ccggtcgttc
     2161 gcgccctggt gcggggaggc gtgaaggtgt ccgtggacac catgaagccc gaggtgatgc
     2221 gccaggccat cgacgcggga tgcgcgatgg tgaacgacgt aaatgcgttc cgggccgaag
     2281 gcgcggccgc catcgtcgcg cggtccgggg taggactgtg cgcgatgcac atgcgtggca
     2341 cgccggccac gatgcaggcc gacccgcaat acgacgacgt gatggccgag gtccgcgcct
     2401 acctcctcga aagggccggg gcgctcgagg cggcgggcgt ttcgcgcgat cgcatcgcca
     2461 tcgaccccgg cttcggtttc ggcaagaact tcgcgcacaa cgtggccctc cttgccggct
     2521 tgccggcgct cgccgcgacg ggctacccgg tggtcgcggg actttcgcgc aagcgcatga
     2581 tcggcgagat cactggacgt cccgtcgacc agcgcgttgc cggcagcgtt gccgcggcac
     2641 tgctggcggt tcagaatggt gcagggatcg tgcgcgtgca cgatgtgaag gaaacggtcg
     2701 atgcgctcca gatctggcgc gcgctcgacg gggtgaggcc atgaaaagca ggcaattctt
     2761 cggcaccgac ggcatccgcg gccgcgtggg cgatccgccg atcacgcccg acttcgtgct
     2821 tcgcctcggc tatgcggcgg gcctcgtcct ctcgcgcgcc gacgttgccc cgccgcaccg
     2881 cgaccgccct gcggtcctca tcggcaagga cacgcgcatc tcgggctaca tgctcgagtc
     2941 cgctctcgag gcggggcttt cggccgcggg cgtggatacg ctgctcgtcg gaccgatgcc
     3001 gacgcccgcg gtggcgttcc tcacgcgcgc gctgcggctt tcggcgggca tcatggtctc
     3061 ggcctcgcac aatccgttcg aggacaacgg catcaagttc ttctccgccg aaggcgccaa
     3121 gcttccggac gcggtggagc acgacatcga ggccgtattg cgcgagccgc tcgtcacccg
     3181 cgattccgcg cacctcggga aggccgagcg cgtgcacgac gccgaagggc gctacatcga
     3241 gttctgcaag ggcaccttcg ccaagcaccg gacgcttcgc gccctcaagc tggtcgtcga
     3301 ctgcgcgcac ggcgcctgcc accggctcgg gccgcgcgtg ttccaggagc tcggtgccga
     3361 ggtggtcgcg atcggcgtgc acccgaacgg cacgaacatc aaccacggct gcggcgccac
     3421 gcacccggcc acgctggcca gggcggtgat cgaccatgac gcggacttcg ggatcgcgtt
     3481 cgacggcgac ggcgaccgcc tcgcgatggc cgaccacgac gggcgcctct tcgatggcga
     3541 ccagctcctc tacgcgatcg cgaagcaccg gcacgagacc ggccgcctcc tcggcggcgt
     3601 ggccggcacg ctgatgacca acttcgccct cgagcggcgc ctgggcgagc tcggcattcc
     3661 gttcgcccgc gcgaaggtcg gcgaccgcta cgtgctcgag ctcctggaag cgaggggctg
     3721 ggaatgcggc ggggagaatt cggggcacat cctctgcctc gactgccact cgacgggcga
     3781 tgcgatcatc tccgcgctgc aggtcctgga ggcgctggtc gcggccggcc agaccctcgg
     3841 cgagtacacg cgcgagctgg cgctgctgcc gcaggtgctc gtgaacgtgc ggctggaacc
     3901 cggcgtggac gcgatgaacg acgcgcgcgt tcgcgaggcg gtcgccagcg ccgaaagcca
     3961 gctcgccggc cgcgggcgcg tgctgcttcg tccctcggga acggagccgc tgatccgcgt
     4021 gatggtcgag ggcgaggatc ccgccagggt gcgggcgctg gccgattcga tcgccgccac
     4081 cgtccgcggc atcgcccagc cggtcgccgg ctgaacgggc gaaaggcgac gcacgggtgc
     4141 ggaagcttcc tcccgaggtc ctcaggggcc tcctgcgcgg catcgagaag gaaggcctca
     4201 gggtccgggc ggatggaatg ctctcgtccg cgccgcatcc ggaagggctg ggcgcggccc
     4261 tgacgcaccc gcatatcacc accgatttca gcgaatccca gctcgaactg atcaccggcg
     4321 tccacgccga tgtcgacgat tgcctgcgcg aactgaccga gctgcaccag gtcgtctacc
     4381 ggcagctcgg aaacgaattg ctctggtgcg cgagcatgcc gtgcaagctg cctcccgacg
     4441 acctgattcc gatcgcgcgc tacggcagct ccaacgtcgc gcgcctgaaa accgtctacc
     4501 ggcacgggct cgcgcatcgc tacggccgcc gcatgcaggt catttccggc atccattaca
     4561 acttctcgct gcccgagtcg gcctgggcgc acctgcacgg cgccgacggc ggcgcggtac
     4621 cggcagacct ctttcgcgac cagcgctacc tggccctgat caggaatttc cggcgccact
     4681 cgtggttgct gctgctgctc ctgggtactt cgcccgccgc ctgcggatcg ttcgttgcgg
     4741 gccgcaacca tggacttgcg gcgtgggaga cgggctcggt gttcgcgccc ggcgcgactt
     4801 cgctgcgcat gggacggctg ggctaccaga gcgatgcgca gtcctcgctc gcggtgagct
     4861 tcaacgacct gggcgggtac gcgaaggccc tgggacgggc gctcaccgaa ccctatcccg
     4921 cctacgaggc cataggcctg cgcgagggcg atgactacct ccagctctcc acgtccctcc
     4981 tgcaactcga gaacgagttc tacggaacca tccggccgaa acggcggacc ctgcccgacg
     5041 agcggccgct gcgggcgctg ggcgagcgcg gcatagagta tgtcgaggtg cggtgcctgg
     5101 atgtcagtcc gttcagcccg gtcggcatcg acgcggacga catgcgcctg ctcgacatct
     5161 tcctgctgca ttgcctgctc ggcgacagcc ccccggattc gccgcgcgag atcgaggcca
     5221 tctcgcgcaa ccagcggctc gtcgcggaaa agggccgcga cccgctcgtc cgcctggatc
     5281 gtgccggggt gcggatcgca ccggccgact ggggcgccga cctgctgctg cagttcgagc
     5341 ctatagccgc ggcgctggac gaggcccacg ggggcgggaa gtatcgcggt gttcttgcgg
     5401 atgcgcggcg cgccctgggc gatctttcgt cactgccttc ggcgcgggtc ctgcgcgaga
     5461 cgcaggcgga acatggaacg tccttcccgg acttcgccct tacgcagtcc ctggtccacc
     5521 aggagacgct tgccgcgctt ccgctggcgg cggaggtcga ggcgcgctac gcgaaagttg
     5581 cgcagcaatc gctggccgag cagcgacgga tcgaggcggg cgacgacgtt cctttcgaaa
     5641 cctatcggca aggctacctt ggccaggaac tgctggccgg gccccacttc cggccgggtc
     5701 actgagcccg ggcggcgaat tccccgaaac cgatgtctac caggcccggg ccatggccgg
     5761 ggcgggttcg tcgatgcatt cctggcagaa gggcgtcgcc ggatcggccc gcagtcgctc
     5821 gggagtaacc tcctcctcgc agcggcagca gatcccgtag gtgccggctt cgaggcggtc
     5881 gatggctgcc tcgaccttgc gccgttcgtt gtccaggcgg ggcatgtgat tcgggccggc
     5941 tttctgcagt tgcgccagca cggcctgaca ttgcatctcg agcagggcct ggaattcggt
     6001 tattgccatg gttgcagcct tttcccgtgg tgggagctgc gcgcattttg ggcgcggcag
     6061 cagtggcata ttgtatccca accggcccct ccccgccggc ggcatttccc gaaatacggc
     6121 gcaattaccc gaatttgcac gacttttcgc cgcgcccccg gctctccgca ccctcggctc
     6181 gcggcgccct cgcttcgccg ggcctgcggg ccccgaaccg ggcggacctc ccctggcgcg
     6241 tccgatcgtg gcgattgccg cgccattgac gtttcaacta tactagaagc atggaaacgg
     6301 agcgcgcgac ggaaatcctc gagtgcctgt cgtcgccgac gaggctgcgg atctaccggc
     6361 tcgtggtcag gcagggcgcc gagggaatgg tcgccggcca gatcgccacg caactcgata
     6421 tcccccccaa tggcgcgtcc ttccacctga aggcgatgac gcatgcgcgc cttttgtcgg
     6481 tgaaacagga ggggcgtttc cagcgctacc gggccgacat cacgcagatg ttcgacctcg
     6541 tggcgtacct gacagaggaa tgctgcgccg gccatcccga gctttgcgct ccggcggccg
     6601 tggtgcgcgt gccggttccc gggttgaagg cgcccgcatc gcgcgccccg agggcgaagc
     6661 gatgaacgtg cttttcctct gcaccggcaa ttcctgccgc tccatcctcg cggaggccgc
     6721 gttcaatcac ctggcgcccg ggggctggcg ggccatgagc gccggcagcc acccgaccgg
     6781 gaccgtccac cctcgctcgc tggccctgct cgcccgcgaa ggcgtatcca ccgagggttg
     6841 ccgcagcaag tcgtgggacg acctgctggc cgtaccggat atcgtggtga ccgtctgctc
     6901 gagcgctgcc ggggaaactt gcccggccta ccttgggccg gttctgcgca cccactgggg
     6961 cgtcgatgac cccgcccatg cgaccggcac cgacgaggaa atcgacgccg cgttcatgaa
     7021 ggcctaccgc atcctgcgcg cgcgcatcga ggcatttttc gcactgcctc tcgaaagcct
     7081 gaagaacgac aaggcgtcgc tcaaggccgg gatggaccgc atcgccaaca tcacctcctg
     7141 aaggcaatca ccatggtcac gattcaagta ttcgaacctg cgttgtgctg cagctccggc
     7201 gtttgcggcg cggatgtcga tccgcaactg gtcgcgtttt cggccgacgc ggactggctc
     7261 gcgaagcagg gcgggcgcat cgagcggctc aatctcgccc agcagcccct ggcctttgcg
     7321 cagaacgcgg tcgtcaagga attcctcgag cgctccgggc aggaagcgtt gcccctggtg
     7381 ctggtggacg caaaggtggc cctggccgga cgctacccga ggcgcgccga gctcgcgcgt
     7441 tggtcgggca tcggcgctcc ggtcgcgcaa gccgaggcgg ccggcggatg ctgtagcggc
     7501 ggccgctgct gctgagcggt atccggcgcg gaaggattcg catgctgttt ctcgagaacg
     7561 tcccgccatt cctcttcttc accggcaagg ggggcgtcgg caagacttcc ctcgcgtgcg
     7621 ccacggcggt gcgattggtg gacggcggaa agcgtgtgct gctggtgagc acggatccgg
     7681 cttccaacgt cgggcaggtc ttcggcgtca cgatcggcaa tgccatcacg gcaatcgatg
     7741 aagtgccggg cctgtccgcg ctcgaaatag atccccaggc agtcgcgcag cagtaccgcg
     7801 accgcatcgt cggtcccgtg cgcggcgtgc tgcccgatgc cgtggtgaag ggcatcgagg
     7861 aacaactctc cggcgcgtgc accacggaga tcgccgcgtt cgacgagttc acggcgctgc
     7921 tgaccgatcg cgcgctgatg ggcaagtacg accacatcgt gttcgacacg gcgcccaccg
     7981 ggcacaccat ccgcctgctg cagttgccgg gggcgtggac cgggttcctg gacgcgggca
     8041 agggagatgc ttcgtgcctg ggcccgctgg ccggcctgga gaagcagcga acgcagtacc
     8101 aggatgcggt gcaggcgctc gcggatcccg agcgtacccg ccttgtcctg gtcgcaaggg
     8161 cccaggattc cgcgctgggt gaagccgcgc gcacccatgg cgagttggcc tcgatcggcc
     8221 tcgggcggca gcacctggtg atcaacggcg tccttgccga gggcgatgtg gccggggacc
     8281 cgatcgccgc ggccatcttc cggcgcgagc gcggcgccat ggacgccatg cccggtgaat
     8341 tgcgtggcct gcctgtcgat cgcgttccgc tcaagggatt caatctcgtc ggcgtgccgg
     8401 cattgcgccg tttttttgcc gacgccgccg cggccgccgc gacaccggcg cccctgtcat
     8461 tgccgctggc agcggagctg gccggactgg tcgacgacat tgcccgggaa gggcacggcc
     8521 tggtcatgct catgggcaag ggcggcgtgg gcaagacgac gctcgccgcc gccgtggcca
     8581 tcgaattggc cggtcgcggc caccccgtcc acctgaccac ctcggatccc gcggcacacc
     8641 tggccgaaac gctcgaaggg accctcgagc acctcgtggt cagccgcatc gatccgcgcg
     8701 ccgaaacgca gcgttaccgc gctcacgtgc tggaggtcaa gggacgcaac ctcgacgagc
     8761 atggccgcgc cctgctcgag gaggacctgc gctcgccctg caccgaggaa atcgcggtct
     8821 tccaggcctt ctcccggatc atccaggagg cggggacgaa gttcgtcgtc atggacacgg
     8881 cgccgaccgg gcacacgctg cttcttctcg acgcgaccgg cgcctaccac cgcgaggtcg
     8941 cgcgccagtt cggcccgggc ggcacgcatt tcaccacccc gatgatgcgg ctgcaggacg
     9001 gcaggcgcac caaggtgctc atcgtcaccc ttgcggagac cactcccgtg ctcgaggcgg
     9061 cggaactgca ggccgacctg cgccgcgccg gcatcgagcc ctgggcgtgg atcgtcaaca
     9121 acagcctggc cttcgcgcgt ccggtttcac cgctgcttcg gatgcgcgcc gcgaacgaga
     9181 tcgcgcagat cgaggccgtg gcgcatcggc atgcccgtcg ctgggcggtc gtccccgtgc
     9241 agccggagga accggtcggc atcgagcgac tgcgcaacct cgcgcgaagc gcccagccgg
     9301 ccacggaaga ggtttgcgat gtccgcgcaa tgtgaagtca ccgcgaagcg cgtttccggc
     9361 gcaccgctcg ggttcttcga gcgctacctg accgcctggg tcgcgctctg catcgtcgtc
     9421 ggcatcatcc tcgggcagtt cctgccggca gtcttccagg cgatcggggc gatggaggtc
     9481 gcgaaggtca acctgccggt gggcgtgctc atctgggtga tgatcatccc gatgctgctg
     9541 aagatcgact tcggcgcgct gcaccaggtg aagggccata tcaggggcat cggcgtgacg
     9601 ctggcgatca actggctggt gaagcccttc tcgatggctt tcctcgcgtg gctcttcatc
     9661 cggcacctgt tcgcaccgct cctccccgcc ggtcagatcg acagctatat cgcgggcctc
     9721 atcctgcttg ccgccgcgcc ctgcaccgcg atggtgttcg tgtggagccg gctcaccaac
     9781 ggagacccgt acttcacgct ctcccaggtg gcgctgaacg acgtgatcat gatcttcgcc
     9841 ttcgcgccgg tggtcggctt cctcctcggc gtggcgagca tcacggtgcc ttgggacacg
     9901 ctcttcacct cggtcgttct ctacatcgtg atcccggtga tcatcgcgca ggtgatccgc
     9961 aggcagctgc ttgcgcgagg gcaggaagcg ttcgacgcgg ccatggcgaa aatccagccg
    10021 tggtccgtct cggcactcct gctcaccctc gtcctgctct tcgccttcca gggcaacgcg
    10081 atactcaagc agccgctggt gatcgcgctg ctggcggtgc cgatcctgat ccaggtcttc
    10141 ttcaactcgt cgctcgccta cctgctcaat cgcgcggtcg gcgaaaagca cagcgtggct
    10201 tgcccttcgg ccctcatcgg cgcctcgaat ttcttcgagc tggcggtggc cgcggcgata
    10261 agcctcttcg gtttcgagtc gggcgcggcg ctcgccacgg tggtaggcgt attgatcgag
    10321 gtgccggtga tgctgctggt ggtgcgcgtg gtcaacgcga cgaagccgtg gtacgaaagg
    10381 ggagcccgcg catgaactgc ctgatgcgcg cctggcgcca gcacgagggg gagcttcgcg
    10441 gctggctgcg caggcgcctg ggcaatcccg ttgacgcaga ggacgccttg caggaacttt
    10501 tcctcaaggc gttgcgccag cgagagcgct tctgcgagat catcaatgcg cgggcgtggc
    10561 tgttcgaggt ggcgcgcaac gccctggccg acaggctgag gctttcgcgg gacatggtcg
    10621 agctgcccga ggatctcgcc gcggccgcgg aggaaaccgc ccccgtcgac agcctggcgg
    10681 gatgcctgcc gcgggtccta tccgaattgt cgccgggcga tcgcgaggcg atcacgctgt
    10741 gcgacctcgg cggcatgccg caggaggagt acgcgcgcct caagggactg agccttcccg
    10801 gcgccaagtc ccgggtgcag cgtgcgcgca agcggctgcg ccagcagctc atgacaggtt
    10861 gccgggtgag cctggatgcg gtggggcagg tgagcgactt cgtgccgcgg ccgccgatcg
    10921 ccggggcaac acggcgcgtt cgtcgcgggg caagacgcga ttgcgacacc tcgtgaaggt
    10981 ccggggttcc cttcgcgcgc gtggccgatg atccggaact cgacggggat acggctgtca
    11041 tggcagttca cccgaacggt tatgcggaga aacccgatgc agacgcgaac gctcaccatt
    11101 gccaaccccg gaggactgca cgcccaggcg tgcgcccgca ttgtcgcgat cgcgaagcga
    11161 ttccgctgca atctttcgat cgtggccagg gggcagcgcg tgcgcgcacg caatatcttc
    11221 gccgtgatgc tgatcaccgc ttcggtgggc gcgacggtcc gcatcgaggc cgacggcccc
    11281 gacgaggaca tggccatgcg ggagatcgcg acgttgtttc acgacggcct gggcgagcgc
    11341 acctaggcgg gatcaacgcg tgcgcgccac gtagacggtg ttgctcgccg ttccgcccgc
    11401 gagggggttg gcaaattcga ccacatgcgc aacggcggcg gcgaacaccg cctcgagttc
    11461 ggaaatgaac tccgcgtcgg gcggatcgtc ggaccacagg gcgaacgagc cgcccgggcg
    11521 aaggtgggcc gcgagcctgc gcagtccgtc ccgccggtag aacgcggcgt ggctcgcgtt
    11581 caggacatgc cggggcgagt ggtcgatgtc gacgagtatc gcgtcgaagc gccgccggct
    11641 gtcgcgtgag tcgatgcccg gcgtgtccac cagcgcgaag aagtcgccat tgaggaactc
    11701 gcagcggagg tccgatgcaa gtcccgcgcc cagagggacg agcccgcggc ggtgccagtc
    11761 gatgacggcc ggcaacgcct ccaccacgag cagcgagccg accttcgcgt ggtccagcac
    11821 cgcgcgcgcc gtgtaaccca gcccgagccc gcccaccacg acatcgagcg ggccgtcgcc
    11881 cagcccgtgc aggccccgtt cggcgagcgc gatttctccg gcggtgaaca ggctggacat
    11941 gaggaaatcg tcgcccagct tgacctcgaa aaggtcgatg cccagcgcgg gttccctgcg
    12001 ccgccgcagg accagttcgc caatcggcgt cgaacgatag tcgagttcct cgagatcggc
    12061 gctcaccgga catcgcctcc ggaaggttcg gcctgcatct ttcggccggg tggttcgtct
    12121 tcactcacga gggtggagtt tgagcccgag tccgagtcga ggaaagtccc gatgagttcg
    12181 gttcccactg cagcgagtcc ctggcccacc cggcggccgg tgccagtcct cgttctggcc
    12241 gcaatcctgt ggttcggcct ctaccaggcg cgggtcccgg tctccgaggg tctggtgtcg
    12301 gccttgcccg tccagcgcga cagccatctg ggcggc
//
LOCUS       DASUEV010000140         6015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_256237, whole genome shotgun sequence.
ACCESSION   DASUEV010000140 DASUEV010000000
VERSION     DASUEV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256237"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..722)
                     /gene="maiA"
                     /locus_tag="VFV55_04575"
     CDS             complement(81..722)
                     /gene="maiA"
                     /locus_tag="VFV55_04575"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX4943604.1"
                     /translation="MILHDYWRSSAAFRVRIALSVKGIEPERRYVHLRKGEQRLPGYL
                     ARHPLGLVPMLEDGAVTLTQSLAIIEYLEETHPEPPLLPAAPAERAWVRAVALAVACE
                     IHPLNNLRVLKYLDRELHADEGARDRWYAHWVAEGLAAIEKMLAARPGPGPFCLGKVP
                     TMADVCLVPQVFNAWRYKVPVEPYPLVRAVVDECMRLPAFDLSQPSKQPDAEE"
     gene            772..1386
                     /locus_tag="VFV55_04580"
     CDS             772..1386
                     /locus_tag="VFV55_04580"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX4943605.1"
                     /translation="MRRPLTRDELIRRLADIPPLEDLLTADDLEKQRLAAAAGELTPA
                     AVLLLVVNHPQGPTVVFTQRTAHLADHAGQISFPGGRVEAGDEGPAHTALREAREEVG
                     IDPAGVEILGELPDYHTSTGYRVRPVVGWVEPPVAWIPDPHEVADVFEVPLAFLLETA
                     NHRYESAFYKGRLRHYWAMPWRGRFIWGATAGMLVTFQRIVGRT"
     gene            1479..2132
                     /locus_tag="VFV55_04585"
     CDS             1479..2132
                     /locus_tag="VFV55_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4943606.1"
                     /translation="MSAQAIKSSKGEETRAQILDAAVQQASAAGFESLTIGALAECTG
                     LSKSGLFAHFGSRTDLQIATLDEASRRFTEAVFLPALKVPRGLKRLHALFENWITWPE
                     HAKLRGSCPMQAASAEYDDQPGPMRDAVAERQRHLARELSKAVQMAVDCGDFRADTDA
                     GQFVFEMFGLVLAYYHTQRLLGDAGAAARARAGFGRLVDAHRATVPKPASRPAAARR"
     gene            2154..3107
                     /locus_tag="VFV55_04590"
     CDS             2154..3107
                     /locus_tag="VFV55_04590"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4943607.1"
                     /translation="MPAVLFSSMNSTIGRSMKMLSTRARLQVGAIVAPERAVADAVRL
                     FLTPPRRLIRDRDRVFLAGGEGFVVAVGEARLSAWRFGPRDRPAVILSHGWGGRGAQF
                     HAFVPALIDAGFQAVVFDHAGHGLSEGRQSSLIDFGRGLGAVANALTLRGIRIAGVVG
                     HSLGAAAIAPFLRASNRSVRSVLVAPPASLVGYSAWFARKLGLGEKLRSRLQSVVEGR
                     YGVRWDELELPASVASLASPALVIHDENDSDVRISAGLAIARAWPGARFVRTRGLGHT
                     AILRDAGVVRDAIDFLKGEVVFARPPAAGEWSAFPGPAPLI"
     gene            3131..3640
                     /locus_tag="VFV55_04595"
     CDS             3131..3640
                     /locus_tag="VFV55_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943608.1"
                     /translation="MKNDEYTDVPATLAVALGLWAGAVAAATRAEIFARLPGEAYAAL
                     VAFAIAFALSVVTMDARVRGWLDLRGMQSASLALLGMAGLMVATGVELARGGAIDPAA
                     APWAPMALFGVPVTLALGAAALGALRREAKPGVTRPASTGPVRRPGATSGSRTSASSP
                     RAARARAAG"
     gene            complement(3534..4166)
                     /locus_tag="VFV55_04600"
     CDS             complement(3534..4166)
                     /locus_tag="VFV55_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4943609.1"
                     /translation="MSAGVLRIWGRLSSVNVQKVVWTADELGLAYERLEAGGAFGVVA
                     TAEYKRMNPNSLVPVIEDDGFVLWESNAIVRYLAAKHAPGTLWPDDLRRRADVDRWMD
                     WQATTFTPAMRDAFWHLLRMPADKRDAAAIEASRAASEKAAAILEAHLAGRAFVSGDA
                     FSPADIVLGCAAHRWLNLPLAREPRGEMTRWYASLKSRPGAAQVLSTPVA"
     gene            complement(4163..5275)
                     /locus_tag="VFV55_04605"
     CDS             complement(4163..5275)
                     /locus_tag="VFV55_04605"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX4943610.1"
                     /translation="MTYAAITGWGKCMPPSILSNADLATIMDTDDAWITSRTGIRERR
                     ISHVSVTTLAHVAAARALACAGLDPADLDLVVLGSCSYDEQVPNTASGVQALLGAGSA
                     AAMDVNTACTSFLYGLSTANAMIRTGVVRNAVVIGVEVISPFMDWNNRNVAVLFGDGA
                     AAVVLQATDERTGLLAEKLGCYADARHILRVRGMGAAYANRGVPLGVTAWDFDGQEIF
                     KRAVRGMTDASAEALQRAGLAIGDIDLVVPHQANLRIIDFVGKRIGADHEHVYLTVER
                     YGNMSAATVPVALVDAIEEGRVKPGARILTPAFGGGLTYCAHVIQWGDRTTPRQASPV
                     ELPPCDKSALEMVRAYMAIKGDAGRAAEGLAAPLAA"
     gene            complement(5350..5817)
                     /locus_tag="VFV55_04610"
     CDS             complement(5350..5817)
                     /locus_tag="VFV55_04610"
                     /inference="COORDINATES: protein motif:HMM:NF040448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HEX4943611.1"
                     /translation="MPKFLRAVRLDDSDDHLFATHGAAREGDWVVSGGYAVCDFANAP
                     KCNPRCHCESSFLALAGRERCSIAEVVEVTTEELAAHTDALAWFLVKGWGAPSWEIAH
                     SLAEEEVNHTADVCETLAPEVWITVKRMPAQGGTAVDEQYAVYDRLLIGSHKL"
BASE COUNT          867 a         2083 c         2131 g          934 t
ORIGIN      
        1 caggttactt ttcagcccgc cgtctagtga cggcaatacg acagaaaagt aacctgaccc
       61 cttttccttg accccttttc ctattcctcg gcgtcgggtt gcttcgaggg ttgcgaaagg
      121 tcgaaggcgg ggagcctcat gcattcatcc acgacggcgc ggacgagcgg gtagggctcc
      181 acgggcacct tgtaacgcca ggcgttgaag acctgcggga caaggcagac gtcggccatc
      241 gtcgggacct tgccgaggca gaacgggccg ggcccggggc gcgcggcgag catcttctcg
      301 atggcggcga gcccctcggc gacccagtgg gcgtaccacc ggtcgcgggc gccctcatcg
      361 gcgtgcaact cgcggtcgag atacttgagt acgcgcaggt tgttgagcgg atggatttcg
      421 caggccaccg ccagtgcaac ggcccgcacc caggcccgct cggcgggcgc ggccggcagg
      481 agcggcggct cggggtgcgt ctcctcgaga tactcgatga tcgcgagcga ctgggtgagg
      541 gtgacggcgc cgtcctccag catcggcacc agcccgaggg gatgcctggc gaggtacccg
      601 ggcaggcgct gctcgccctt gcgcaggtgc acgtagcggc gttccggctc gatgcccttg
      661 acggaaagcg cgatgcgcac gcggaaagcg gcggaagacc gccagtaatc atggagaatc
      721 atggtgggcg catcttatac ttgggctgga caccgtgaaa ccaccgcacc gatgcgccgc
      781 ccgctgaccc gcgacgaact catccgtcgc cttgccgaca tcccgcccct cgaggacctg
      841 ctgaccgcgg acgacctcga gaagcagcgg ctggcggccg cggcgggcga gctcacgccg
      901 gccgccgtgc tgctcctcgt ggtgaaccat ccgcaggggc ccacggtcgt cttcacgcag
      961 cgcacggcgc acctcgcgga ccatgccggg cagatcagtt ttccgggcgg gcgcgtggag
     1021 gcgggcgacg aaggcccggc acacacggcg cttcgcgagg cgcgcgagga agtgggcatc
     1081 gatccggccg gcgtcgagat cctgggggag ctccccgact accacacgtc caccggctat
     1141 cgcgtccgcc ccgtggtggg gtgggtcgag ccgcccgtcg cgtggatccc ggacccgcac
     1201 gaggtggccg acgtcttcga ggtgcccctc gcgttcctcc tcgagacggc caatcatcgc
     1261 tacgaaagcg ccttctacaa gggacggctt cgccactact gggcgatgcc gtggcgcggg
     1321 cgcttcatct ggggcgccac cgcgggaatg ctcgtcacct tccagcgcat cgtggggaga
     1381 acctaaaggg gtcaggttac tttgcttaat ttttgtgcaa agtaacctga cccctttagc
     1441 acttgacaag atacgaacga tcgtactatt ttgtcgccat gtcagcgcaa gccatcaagt
     1501 ccagcaaggg ggaagagacc cgcgcccaga tcctcgacgc cgccgtgcag caggcgagcg
     1561 cggcgggatt cgagtcgctc accatcggcg cgcttgccga gtgcacgggc ctgtcgaaga
     1621 gcgggctctt cgcgcacttc gggtcgcgca cggacctgca gatcgccacg ctcgacgagg
     1681 cctcgcgccg cttcaccgag gccgtcttcc tgcccgccct gaaggtgccg cggggcctca
     1741 agcgcctgca cgcgctgttc gagaactgga tcacgtggcc cgagcacgcc aagctgcgcg
     1801 gcagctgccc catgcaggcg gcctcggccg agtacgacga ccagcccggt ccgatgcgcg
     1861 atgcggtggc cgaacgccag cggcaccttg cgcgcgagct gtcgaaagcc gtgcagatgg
     1921 cggtcgactg cggcgatttt cgcgccgaca ccgacgcggg gcagttcgtc ttcgagatgt
     1981 tcggcctcgt gctggcctac taccacacgc agcgcctgct cggcgacgcg ggggccgccg
     2041 cccgcgcgcg cgccggcttc gggcgcctgg tggacgccca ccgcgcaacc gtcccgaaac
     2101 ccgcatcccg ccccgcggcc gcgcgccgct gatccctgac tgacggaggt cccatgcccg
     2161 ccgttctctt ctcgtccatg aatagcacga tcggtcgttc catgaagatg ctgtcgacgc
     2221 gcgcccgcct gcaggtgggg gccatcgtgg cgcccgagcg cgccgtggcc gacgcggtgc
     2281 gcctgttcct cacgccgccg cgccggctca tccgcgatcg cgaccgggtc ttcctggccg
     2341 gcggcgaagg cttcgtcgtc gccgtcggcg aagcgcggct ttcggcgtgg cggttcggcc
     2401 cgcgcgaccg tcccgcggtg atcctgagcc acggctgggg cggacgaggc gcgcagttcc
     2461 acgcgttcgt tccggcgctg attgacgcgg gcttccaggc ggtcgtgttc gaccatgccg
     2521 gccacggcct gtccgagggg cggcagtcct cgctcatcga cttcggccgc gggctcggcg
     2581 cggtggccaa cgcgctcacc ttgcgcggca tccgcatcgc gggcgtcgtg ggccattcgc
     2641 tgggcgcggc cgccatcgcg ccgttcctgc gcgcctcgaa ccgcagcgtg cgcagcgtgc
     2701 tcgtggcgcc gccggcctcg ctcgtgggct attccgcgtg gttcgccagg aagctcgggc
     2761 tgggcgagaa gctgcgctcg cgcctgcagt cggtcgtcga aggccgctac ggcgtgcgct
     2821 gggacgaact cgagctgccg gcctccgtgg cctcgctcgc gtcaccggcg ctcgtgatcc
     2881 acgacgaaaa cgattccgac gtgcgcatca gcgcgggact tgccatcgcc cgcgcatggc
     2941 ccggcgcgcg cttcgtgcgc acccggggcc tcggccacac cgcgatcctg cgcgatgcag
     3001 gcgtggtgcg cgacgccatc gacttcctca agggcgaggt ggtctttgcc cgaccgcccg
     3061 cggccggcga atggtccgcc tttcccggtc ccgcgcctct catctgatgc gacgactaag
     3121 gagcagagcc atgaagaacg acgaatacac ggatgtgccc gccacactcg ccgttgcgct
     3181 cgggctgtgg gccggagccg tcgccgcggc gacgcgcgcc gaaatcttcg cgcggctgcc
     3241 cggggaggcc tacgccgcgc tggtcgcctt cgcgatcgcc ttcgcgctga gcgttgtcac
     3301 gatggacgcg cgcgtgcgcg ggtggctcga cctgcgcggc atgcaatccg catccctggc
     3361 gctgctcggg atggcagggc tcatggtggc tacgggggtc gaactcgccc gcgggggcgc
     3421 gatcgacccg gctgctgcgc cgtgggcacc catggcgctc ttcggcgtgc ccgtcacgct
     3481 ggcgctcggg gccgcggcgc tcggggcgct gcggcgcgag gcgaagcccg gcgtcacgcg
     3541 accggcgtcg acaggacctg tgcggcgccc gggcgcgact tcaggctcgc gtaccagcgc
     3601 gtcatctccc cgcgcggctc gcgcgcgagc ggcaggttga gccagcggtg cgcggcgcaa
     3661 cccagcacga tatcggcggg agaaaaggcg tcccccgaca cgaatgcgcg tcccgccagg
     3721 tgcgcctcga ggatggcggc ggccttctcc gaggccgccc gtgaagcctc gatcgccgcg
     3781 gcatcgcgtt tgtccgcggg catgcgcagg aggtgccaga aggcgtcgcg catcgcaggc
     3841 gtgaaggtgg tcgcctgcca gtccatccag cgatcgacgt cggcgcgtcg cctcaggtcg
     3901 tcgggccaca aggtgcccgg cgcatgcttc gcggcgaggt agcgcacgat cgcgttcgat
     3961 tcccacagca cgaagccgtc gtcctcgatc accgggacca ggctgttggg gttcatgcgc
     4021 ttgtactcgg cggtcgcgac gacgccgaag gcgccgccgg cctcgaggcg ttcgtaggcg
     4081 agccccagtt cgtcggccgt ccacaccacc ttctggacgt tgaccgagga gaggcgcccc
     4141 cagatgcgca gcacgcccgc gctcacgcgg cgagaggcgc ggccagaccc tcggcagcgc
     4201 ggccggcgtc gcccttgatc gccatgtagg cgcgcaccat ctcgagggcc gacttgtcgc
     4261 agggcggcaa ctcgacgggc gaggcctgcc tcggcgtcgt ccggtcgccc cactggatca
     4321 cgtgcgcgca ataggtgagg ccgccgccga aggcgggggt gaggatgcgc gcgcccggct
     4381 tcacccggcc ttcctcgatg gcatcgacca gggcgaccgg aacggtggcc gccgacatgt
     4441 tgccgtagcg ctcgacggtc aggtatacgt gctcgtgatc cgcgccgatc cgcttgccca
     4501 cgaagtcgat gatgcgcagg ttggcctggt gcggcaccac caggtcgatg tcgccgatgg
     4561 cgagcccggc gcgctgcagc gcctcggcgg aagcgtccgt catgccgcgc acggcgcgct
     4621 tgaagatttc ctgcccgtcg aagtcccagg ccgtcacgcc aagggggacg ccgcggttgg
     4681 cgtaggccgc gcccatgccg cgcacgcgca ggatgtgccg cgcgtcggca tagcagccca
     4741 gcttttccgc gagcaggccg gttctctcgt cggtggcctg caggaccacg gccgcggcgc
     4801 cgtcgccgaa gagcaccgcc acgttgcggt tgttccagtc catgaagggc gagatcacct
     4861 ccacgccgat cacgaccgca ttgcgcacga cgcccgtgcg gatcatcgcg ttggcggtgg
     4921 acagtccgta gaggaagctg gtgcaggcgg tgttcacgtc catcgcggcc gcgctgccgg
     4981 cgccgaggag cgcctgcacg ccggaggcgg tgttgggcac ctgctcgtcg taggagcagc
     5041 tgccgagcac caccaggtcg aggtcggcgg ggtcgagccc ggcgcaggcg agcgcccgcg
     5101 cggcggccac gtgggcgagc gtggttacgg aaacgtgcga aatgcgcctt tcgcggatgc
     5161 cggtgcgcga cgtgatccac gcgtcgtccg tatccatgat cgtcgcgagg tcggcattgg
     5221 aaaggatgga cgggggcatg cacttgcccc agccggtgat ggctgcgtag gtcatttttc
     5281 gtggggtccg tgaagcggtg tggggagagt gtgcacgagt cggcgaccgg gcgcttgccc
     5341 ggtaccggct cagagcttgt gcgatccgat gaggagccgg tcgtagacgg cgtactgttc
     5401 gtccaccgcg gttccgcctt gcgccggcat gcgcttcacg gtgatccaca cttccggggc
     5461 gagcgtctcg cagacgtccg cggtgtggtt cacctcctcc tcggccaggc tgtgcgcgat
     5521 ctcccaggac ggggcgcccc agcccttcac gaggaaccag gccagcgcgt cggtgtgcgc
     5581 tgccagttcc tcggtcgtca cctcgaccac ctcggcgatc gagcaccgct cgcgtcccgc
     5641 gagcgcgagg aaggacgact cgcagtggca gcgcggattg cacttcgggg cgtttgcgaa
     5701 gtcacacacc gcgtagccgc cgctcaccac ccagtcgccc tcgcgggcgg cgccgtgggt
     5761 ggcgaagagg tggtcgtcgg aatcgtcgag gcggacggcg cgcaggaact tcggcatgga
     5821 gcgtgcttcg ctggagcgaa ggtgcccgta tcatagcagc cggccccacg gaagattcgc
     5881 ccagaggaga gaaatggcca ttgcaaagac cgccatcgtc accggtgcca accgggggat
     5941 cggcctcgag atcacccgcc agttcgtgcg ggaaggcatt cgcgtcgtca tgggcgtgcg
     6001 cgacccggcg aaggg
//
LOCUS       DASUEV010000141         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_148942, whole genome shotgun sequence.
ACCESSION   DASUEV010000141 DASUEV010000000
VERSION     DASUEV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148942"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1274
                     /gene="nqrF"
                     /locus_tag="VFV55_04615"
     CDS             48..1274
                     /gene="nqrF"
                     /locus_tag="VFV55_04615"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEX4943612.1"
                     /translation="MTEIVLGVVMFTAVILALIALLMAARSKLVATGDVTITINEDPD
                     KALRVPAGGTLLAKLAESQIFIPSACGGKGACGVCEVVVKEGGGEILPTETGYISRGD
                     AKRGCRLACQVKVKGDMAIEIAPEVFSVRKWKCRVISNRNVATFIKELKLALPEGEEV
                     PFNAGGYVQIECPPHSLSFRDFSIEEPFRADWDKFDLWRFKSSCAAPVTRAYSMANYP
                     MEKGILLFTIRIAFPPGYQESIPPGILSSWVFNLKEGDEVTVSGPFGEFFARDTNAEM
                     CFIGGGAGMAPMRSHIFDQFHRLHTKRKATYWYGARSLREAFYQDEFDAIAKAHPNFE
                     WHLALSEPLPEDNWTGYTGFIHKVLHDEYLKNHPAPEDIEYYMCGPGPMTKAVIDMLL
                     DLGVERENIMLDDFGS"
     gene            1292..1741
                     /locus_tag="VFV55_04620"
     CDS             1292..1741
                     /locus_tag="VFV55_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4943613.1"
                     /translation="MTVATVMDALREKGMETLHSIAASANVEEAVEQMAANEIGALVV
                     STEDALVGGIFTERDVMMRVVRAGLDPKRTPLSLVMTRDVHFVSPGTTLEAALALMYV
                     HRHRHLLVIDGPHVHGLVSMRDLAYQLILHGEGRFEAAVRLAGGPGT"
     gene            1759..2820
                     /locus_tag="VFV55_04625"
     CDS             1759..2820
                     /locus_tag="VFV55_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX4943614.1"
                     /translation="MDTLLSPRRRTVIQALGACAFLAGCGEVPGITRRPLAVFQGPAM
                     GTLWNAKLAGPLAEGLAAKASTAVAAALEGVVARMSTYLDTSELSLFNRHPEATPLAV
                     SRETLEVFALAREVSEASGGAFDVTVAPAVDAWGFGPSKAQRIPAAAELAAMASRIDW
                     RAIAIDRGAGTLAKSRDGVRADLSGIAKGYGVDLAARGLDALGVADYLVEAGGELRAR
                     GRNADAKPWRIGIERPDAVPQQPLLVVPLDGLSMATSGDYRIYFEQGGRRYSHEIDPA
                     TREPVRGSLASVTVVARDCAWADAMATALFVMGTARGLEFATGRGMAACFIERTGKGK
                     FVDRQTPAFARLGAEHVRA"
     gene            2829..3017
                     /locus_tag="VFV55_04630"
     CDS             2829..3017
                     /locus_tag="VFV55_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943615.1"
                     /translation="MDSTLVAVFLVAFVLIALAVGGMAMGSRMTGRCLRGSCGGPAVV
                     GKDGKEIGSCAGCPNRKP"
BASE COUNT          511 a         1155 c         1095 g          535 t
ORIGIN      
        1 cgtcgccttc atgatgttct ccgggatcca gctctagggc cgccgccatg accgaaatcg
       61 tcctcggcgt cgtcatgttc accgcggtga tcctcgcgct catcgcgctg ctcatggcgg
      121 cccgctcgaa gctcgtggcc acgggcgacg tgaccatcac catcaacgag gacccggaca
      181 aggcgctgcg ggtgccggcc ggcgggacgc tgctcgcgaa gctggcggag agccagatct
      241 tcattccctc tgcctgcggc ggcaagggcg cctgcggcgt gtgcgaggtg gttgtgaagg
      301 aaggcggcgg cgagatcctg cccacggaga ccggctacat ctcgcgcggc gacgccaagc
      361 gcggctgccg cctcgcctgc caggtcaagg tgaagggcga catggcgatc gagatcgccc
      421 ccgaggtctt cagcgtgcgc aagtggaagt gccgcgtgat ttccaaccgc aacgtggcca
      481 ccttcatcaa ggagctgaag ctggcgctgc ccgagggcga ggaagtgccc ttcaacgcgg
      541 gcggctacgt gcagatcgag tgcccgccac actcgctttc cttccgcgac ttctcgatcg
      601 aggagccgtt ccgcgccgac tgggacaagt tcgacctgtg gcgcttcaag tcgagctgcg
      661 ccgcgcccgt gacgcgcgcc tactcgatgg ccaactaccc gatggagaag ggcatcctcc
      721 tcttcaccat ccgcatcgcc ttcccgccgg gctaccagga gagcatcccg ccgggcatcc
      781 tgagctcgtg ggtgttcaac ctgaaggaag gcgacgaggt cacggtatcc ggccccttcg
      841 gcgagttctt cgcgcgcgac acgaacgcgg agatgtgctt catcggcggg ggcgcgggca
      901 tggcgcccat gcgctcgcac atctttgacc agttccaccg tctgcacacg aagcgcaagg
      961 cgacctactg gtacggagcg cgcagcctcc gggaggcgtt ctaccaggac gagttcgacg
     1021 ccatcgcgaa ggcccacccc aatttcgagt ggcacctcgc gctatcggag ccattgcccg
     1081 aggacaactg gaccggctac acgggcttca tccacaaggt cctccacgac gagtacctga
     1141 agaaccaccc ggcgcccgag gacatcgagt actacatgtg cggcccgggg ccaatgacca
     1201 aggccgtgat cgacatgctg ctggatctcg gcgtggagcg cgagaacatc atgctcgacg
     1261 atttcggcag ctgacggcga ggagacggac gatgacggta gccaccgtga tggatgccct
     1321 gcgcgagaag ggcatggaga cgctgcactc gatcgccgcc tcggcgaacg tggaagaggc
     1381 cgtcgagcag atggccgcca acgagatcgg cgccctggtg gtgagcaccg aggacgcgct
     1441 ggtcgggggc atcttcaccg agcgcgacgt gatgatgcgc gtggtgcgcg ccgggctcga
     1501 cccgaagcgc acgccgctct ccctggtgat gacgcgcgac gtgcacttcg tgtcgccggg
     1561 aaccacgctc gaggccgcgc tcgccctcat gtacgtgcac cgccaccgcc acctgctcgt
     1621 gatcgacggg ccgcacgtgc acggcctggt ctcgatgcgc gacctcgcct accagctgat
     1681 cctgcacggc gagggccgct tcgaggcggc ggtacgcctc gccggcgggc cgggcacctg
     1741 actgccaagg aagacaccat ggatacgctt ctctcccccc gccgccgcac cgtcatccag
     1801 gcgctcggcg cctgcgcgtt cctcgccgga tgcggcgagg ttccgggcat cacgcgccgg
     1861 ccgctcgccg tcttccaggg cccggccatg ggcaccctct ggaacgcgaa gctcgccggc
     1921 ccgctggccg agggcctcgc ggcgaaggcc tcgaccgccg ttgccgccgc gctcgagggc
     1981 gtggtggcgc gcatgtcgac ctatctcgac acctcggagc tttcgctctt caaccgccac
     2041 ccggaagcga caccgcttgc cgtctcgcgc gagacgctgg aggtcttcgc actggcccgc
     2101 gaggtgagcg aagcgtccgg cggcgccttc gacgtgaccg tggcgcccgc cgtcgatgcc
     2161 tggggattcg gaccgtcgaa ggcgcagcgc atcccggccg ccgcggaact ggccgcgatg
     2221 gcgtcgcgca tcgactggag ggcgatcgcg atcgatcgcg gcgcgggcac gctcgccaag
     2281 tcccgcgacg gcgtccgcgc cgatctctcc ggcatcgcca agggctacgg cgtggacctc
     2341 gcggcacggg gcctcgatgc cctgggcgta gccgactatc tcgtggaagc cggcggcgag
     2401 ttgcgcgcgc gcggccgcaa tgccgacgca aagccgtggc gcatcggcat cgaacgcccg
     2461 gacgccgttc cgcagcagcc cttgctcgtg gtgccgctcg acggcctctc catggccacc
     2521 tccggcgact accgcatcta cttcgagcag ggtggccgcc gctattccca cgagatcgac
     2581 ccggccacgc gcgagccggt tcgcggaagc ctggcctcgg tcaccgtcgt cgcgcgcgac
     2641 tgcgcctggg ccgacgccat ggccaccgcc cttttcgtga tgggcaccgc gcgcggcctc
     2701 gaattcgcca ccggtcgcgg aatggccgcc tgtttcatcg agcgcaccgg caaaggcaaa
     2761 ttcgtcgacc ggcagacgcc cgccttcgca cggttgggcg ccgagcacgt cagggcctga
     2821 ggatcaccat ggacagcact ctcgtcgccg tcttcctcgt cgccttcgtg ctcatcgcgc
     2881 tggcggtagg cggcatggcg atgggctcgc gcatgacggg ccgctgcctg cgcggctcgt
     2941 gcggcgggcc ggccgtcgtc ggcaaggacg gcaaggaaat cggctcctgc gcaggctgcc
     3001 ccaaccgcaa gccgtaaagc cgcgccgaga ttggcacgac gaagttcggg gttcagcctc
     3061 cgcaagcgcg gtcggcgatc aggacgacga agttcggggt ctggttggct atgttttttt
     3121 cgaggaaaag gggtcaggtt actttacagc ccggtcgaaa tccttcgaca gtaaagtaac
     3181 ctgacccctt ttcctcacgg ctacgatttg ctcttgcgcg ccttcggctt gggtcgcgct
     3241 ttttccggcg ctgcctccgg ttcggcttcc ttcgcggcgc ccttcgccgc ctcctg
//
LOCUS       DASUEV010000142         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1072505, whole genome shotgun sequence.
ACCESSION   DASUEV010000142 DASUEV010000000
VERSION     DASUEV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072505"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..2191)
                     /locus_tag="VFV55_04635"
     CDS             complement(206..2191)
                     /locus_tag="VFV55_04635"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4943616.1"
                     /translation="MTATAWLRRHVEAILAFGLALLFTAAALWLPAWQALERRGFDAL
                     TVLAAPGESTQPIVLVAINEESFEALKHRWPWPRSVHAQLLDRLVAGGAAVVAFDVLL
                     ADASDAGDDLAFAEAIRRAGNVVLAADFTYAETHAARLWKRTEPLELFIRAGAVAGLA
                     TVPLDPDQFVRNIPVEPDAFWRQIVKLLQVRVPSLAVPPLPEPGAMIRYLGGEGMYPA
                     IPYHLVLAATPEELKGAFEGRIVIVGRNLRAAPELGMAQGDLFATPFLSDAGGLQAGI
                     KIHATLVENALAGIAIRPGGAFAILLANLVAALGVALAFRRAKPHIAAVVAALLVAAF
                     SGIAWLAFSRANLWIPFAAPMAVVLATWLMNLARAYLSERARKQEVHQAFSMYVAPTV
                     VDQLLADPKRLALGGEAREITVMFTDLAGFTKLTEKTAPDIVSRVLTEHFTAMTDIVL
                     AHGGTVIQFIGDAIMAFWGAPLDDREHRRHATQAAVAMQRGMANLRRDFAAQGLPEIH
                     MRVGLNTGTAVVGNMGSRRRFAYSALGDCVNLASRLEGANKLYGTGILMSGETAKGAG
                     GAMAFRHVDRVRVAGKTEAVDVYTPCDDPAFIAASEIALSATFARDWDGAEGAWRGLL
                     AGHPDDRGIGLHLQRIAAWRQSPPPVGWDGGVSIDKL"
     gene            2505..>3246
                     /locus_tag="VFV55_04640"
     CDS             2505..>3246
                     /locus_tag="VFV55_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943617.1"
                     /translation="MRPDQIARNAGRGSLAIGLAGLIALFLVTLFPADLAAQSAGVTR
                     ITSSFGTGPKPVGFYEFSGTFTCDYLSGETGPCTGRFVGVERDETCSNDFQFSGDMSI
                     TGIDLSRTGPFNGVVTFTHIDFHSTTRPDGSCSFVLGPQNIEVKPFTGTWNGTTGTFE
                     FPTDRDIHGNLIVLTGSFTAYRNAPVPVFPMTVRSNISSTSATATADIQFRPQDVGAS
                     GSIYSFAVAPSALVKGGQDAKAVAIGSAW"
BASE COUNT          568 a         1096 c         1069 g          513 t
ORIGIN      
        1 ctcgagcgac tgcttcacct tcgccgccac ccaatcccgg gcgccgccat cgcggtactc
       61 gcccttgccg gtgaggctca cgatctcgcc ggccggcaca gccagcgcgg ccggcacgaa
      121 aagggaaagc gagagtccga gaagaataac gtggcgtttc atggcgggcc ttccgttggg
      181 aaatcggatt gggtgcggat ccgggtcaga gcttgtcgat cgagacaccg ccatcccatc
      241 caacgggcgg cggcgattgg cgccaggcgg cgatgcgctg gagatgaagc ccgatgcccc
      301 tgtcgtcggg gtggccggcg aggagcccgc gccacgcgcc ttccgcgccg tcccagtcgc
      361 gcgcgaatgt cgcggatagc gcaatctcgc tcgcggcgat gaacgccgga tcgtcgcacg
      421 gcgtgtacac gtccacggcc tcggtcttcc ccgcaacgcg cacccggtcg acgtggcgaa
      481 acgccatggc gccgcccgcg cctttcgcgg tttctccgga catgaggatc cccgtgccgt
      541 acagcttgtt ggcgccttcc agtcgcgaag cgaggttcac gcagtcgccg agcgccgaat
      601 aggcaaagcg ccgccgcgag cccatgttgc cgacgaccgc cgtgccggtg ttgagtccga
      661 cgcgcatgtg tatctccggc aggccctgcg ccgcgaagtc cctgcgcagg ttcgccatcc
      721 cccgttgcat cgcgacggcc gcctgcgtcg catggcgacg gtgctcgcgg tcgtccagcg
      781 gcgcgcccca gaacgccatg atcgcgtcgc caatgaactg gatcaccgtg cctccgtgcg
      841 cgaggacgat atccgtcatc gccgtgaaat gctcggtgag cacgcgcgaa acgatgtccg
      901 gcgcggtctt ctcggtgagc ttcgtgaagc cggccaggtc ggtgaacatg accgtgatct
      961 cgcgggcctc gccaccgagc gcgaggcgct tcgggtccgc gaggagctgg tccacgacgg
     1021 tcggcgccac gtacatcgaa aaggcctggt gcacctcctg cttgcgcgcc cgctcggaga
     1081 gataggcacg cgcgaggttc atgagccagg tggcaagcac gaccgccatc ggcgccgcaa
     1141 acggaatcca caggttcgcc ctcgagaagg ccagccaggc gatcccggaa aacgcggcca
     1201 cgagcaacgc agcgaccaca gccgcgatgt ggggcttcgc acggcggaag gcgagggcca
     1261 cgccgagcgc ggccacgagg ttcgcgagca ggatcgcgaa tgccccaccg ggccggatcg
     1321 cgatgcccgc cagcgcattt tccacgagcg tcgcgtgaat cttgattccg gcctgcaagc
     1381 cgcccgcatc ggagagaaac ggggtcgcga agagatcccc ctgcgccatg cccagctccg
     1441 gcgcagcgcg cagattgcgg ccgacgatca cgatgcggcc ctcgaacgcc cccttgagtt
     1501 cttccggggt ggccgcaaga acgaggtgat agggaatcgc ggggtacatg ccctccccgc
     1561 ccaggtagcg aatcatggcc cccggttccg gcagcggcgg cacggcgagg gaaggcactc
     1621 gcacctgcag cagcttgacg atctggcgcc agaacgcgtc gggttccacc ggaatgttgc
     1681 gcacgaactg gtcgggatcg agcggcaccg tcgcgagccc tgcgacggcg ccggcgcgga
     1741 tgaacaattc aagcggctcc gtgcgcttcc acaggcgggc cgcatgcgtc tcggcgtagg
     1801 tgaaatcggc ggcgagcacc acattgccgg cacgacgaat cgcctcggcg aacgcgagat
     1861 cgtccccggc atcggaggcg tcggccagca gcacgtcgaa ggccaccacc gccgcgccgc
     1921 ccgccaccag gcggtcgaga agctgcgcat gcacgctcct tggccacggc cagcgatgct
     1981 tcagggcttc gaacgattcc tcgttgatcg ccacgaggac gatgggctgg gtcgactccc
     2041 cgggagccgc caggacggtg agcgcatcga agccgcggcg ctcgagcgcc tgccacgccg
     2101 gcaaccagag ggctgccgcg gtgaacagca gcgccagccc gaatgcgagt atggcttcga
     2161 cgtggcggcg aagccatgcg gtagcggtca agtcggcttt accctcaagg ggtgatcgga
     2221 ggtttcccgg ccccggggtc cgggcccatg ggcacgatgt tgcggcgaag tcgccggaac
     2281 cgggagatcc cgcatggccg ccgcggcacg aaatgttgcc tatggttgat atggatcaaa
     2341 gacccgagtc gctgcggatg cgccgccctt gtcaacgagt cgggttcgac ggttaatgac
     2401 ttctaccctt gttccctccc agggactgac cgtgcagcag gccgccctgc tcccggcagg
     2461 acaagaagat cgtggtgaat gcgcaccgga aggagtcctc cgtcatgaga ccggatcaaa
     2521 ttgcccggaa tgccggccgg ggatcgcttg cgatcggcct ggccggactg atcgccctgt
     2581 ttctcgtgac cctctttccg gccgaccttg ccgcccagtc cgcgggcgtg acgcggatca
     2641 cgtcctcgtt cggaaccggt ccgaagccgg tgggtttcta cgagttttcc gggaccttca
     2701 cgtgcgatta cctcagcggg gaaacaggcc cttgcaccgg caggttcgtc ggagtggaga
     2761 gggacgaaac ctgcagcaac gatttccagt tcagcggcga catgtccatc acgggaatcg
     2821 acctgtcccg gaccggcccc ttcaatggcg tggttacgtt cacgcacatc gattttcatt
     2881 cgaccacgag gcccgacggc tcatgctcgt tcgtcctggg tccccagaac atcgaagtaa
     2941 agccctttac cggcacctgg aacgggacga cgggcacatt cgaattcccg acggaccggg
     3001 atatacatgg caatttgatc gtccttacgg gctcgttcac tgcctaccgc aacgcgccgg
     3061 tgcccgtgtt tcccatgacg gtgagatcca atatcagcag cacatccgcc acggccacgg
     3121 ccgacatcca gttccggccg caggacgtgg gcgcctccgg cagcatctac tcgtttgccg
     3181 tggcgccatc ggcattggtc aagggcggcc aggacgcgaa ggccgtagcc atcggctcgg
     3241 cctggt
//
LOCUS       DASUEV010000143        11932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_843210, whole genome shotgun sequence.
ACCESSION   DASUEV010000143 DASUEV010000000
VERSION     DASUEV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11932
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843210"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="VFV55_04645"
     CDS             <1..2079
                     /locus_tag="VFV55_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX4943618.1"
                     /translation="DAGATNGQVTLVGLAQRGEKAAAIERASATIKGTRSSHEIRVEA
                     DFPDKSTARALFDGGIVAGARLPEWRGRLESLETAGRASFALAAPAALLVSADRIELG
                     EALFAGEPGEVRLAVTRWTPAGVESRGSSSALVIQSIRRILNLQGQVGSNLVLAGNWD
                     LRVGESVDGFVSLRRERGDLRMGEPRQALGLEVLALRADASGGRVRVTADVRGKQAGQ
                     WTGELSVMLRNGSEGWEISPVAPIEGRFSVDVPDLAWIAAWIGPEARAGGRLKGEATL
                     AGTAREPAWSGRVEANGLVLREPALGAEVADGTIAIVLRDREARIERFALSMPWQPPD
                     EAARAIAAARRPATGTVTAEGAVDLGTRKGTIHLKAVAWPLTRLATRFLAVSGEGRAE
                     LDGNVTVMTGAFTADAGWFGIPASAPPSLSDDVIVDRGGQAPVVAKGAERIRLDLRVD
                     LGEHLHFRGRGLATRLAGSLRLAGDVGANLRTTGTIQAMGGTYDAYGRSLAIERGALN
                     FQGPVDNPGINVLALRKGLPVEAGVEVLGTVARPRVRLVSSPDVPDPEKLTWLVLGRG
                     QGDVSAGDAAVLVGAANALLGRDMPASAKLLGGLGLDDVQLGRDSSGTLGTLPQSTVA
                     GRTGAASAAEVVTVGRRLTDDIYVSYRQGLADAEGSLRVAWQLTRSLQIILRAGHLPG
                     IDAVYRFSFD"
     gene            complement(2082..2489)
                     /locus_tag="VFV55_04650"
     CDS             complement(2082..2489)
                     /locus_tag="VFV55_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX4943619.1"
                     /translation="MSNARKGKYGYSRIVALTYEQAVERVTATLKEQGFGILTEIDVK
                     ATLKKKLDKDFTKYVILGACNPNLAFQALSGEIDIGLLLPCNVTVYENPADGKTVVSV
                     LDPETMVTLTGRQDIQPLAREARDKVVAALDAM"
     gene            2674..3018
                     /locus_tag="VFV55_04655"
     CDS             2674..3018
                     /locus_tag="VFV55_04655"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4943620.1"
                     /translation="MAARQPVEDVSRYADMLSAMGTEPRLRIMRLLLSAHPEGLVVGD
                     VGAELGIANSTLSHHLERLRNEGLVNVHREGTFLRYSANAGALQDLLRFLYAECCTRS
                     GACEPKKIVSAN"
     gene            3033..3911
                     /locus_tag="VFV55_04660"
     CDS             3033..3911
                     /locus_tag="VFV55_04660"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX4943621.1"
                     /translation="MSEPNDVKEAVRERYSQAALRVKGGGGGCCAPAPAATGCCGSAP
                     AATSCCGTARAATSSDDPITSDLYDAAQAGAVPEAALQASLGCGNPTALAELKPGETV
                     LDLGSGGGIDVLLSARRVGPTGKAYGLDMTDEMLALAEENKRRSGLANVEFLKGEIES
                     IPLPDNTVDVIISNCVINLSGDKDRVLREAFRVLKPGGRFAVSDVVVRGEVPAAIRKS
                     LELWAGCIAGALSETDYVRKLSDAGFTDIGVEVTRVYGGADAQEFLAAGGFDAQAIAK
                     EVDGKFVSAFVRARKP"
     gene            4171..4290
                     /locus_tag="VFV55_04665"
                     /pseudo
     CDS             4171..4290
                     /locus_tag="VFV55_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
     gene            4287..5009
                     /locus_tag="VFV55_04670"
     CDS             4287..5009
                     /locus_tag="VFV55_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4943622.1"
                     /translation="MTIHDVTLAAREEVAEGTMAFHFAKPAGFVFKPGQAIDLILPGL
                     PDANGQPSRHAFSIVSAPFERDLVVATRMRDSAFKRALGAMPIGAPVQVDGPFGSLTL
                     HNDRARAAVFIAGGIGITPFMSMLRHAAADPIPRSLTLLYSNRRPEDAAFLAELQQLE
                     KRNRNFRLVATMTAMARSGRPWEGEVRLIDADLVRKVSAGLTTPVYYLVGPPALVEGL
                     RKALGEAQIDDDDVRSEEFYGY"
     gene            5030..5911
                     /locus_tag="VFV55_04675"
     CDS             5030..5911
                     /locus_tag="VFV55_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4943623.1"
                     /translation="MNAESGTARADHSPTAFVLAGGGSFGAVQVGMLRALTAHGVVPD
                     LVVGSSVGAINGAFFAGAPGSEGVAQLEAIWCGLHRQEVFPFTWRCLVGLLRRRNFVV
                     DPGGLRGVLERYLQFRELEHAALPLHVVATDQLGGGTVRLSSGPAVEAVLASCAIPAA
                     FPPVFIGERYLIDGAVASNTPIGVAVELGAKRIVVLPTGFACALEAPPRGVIASALHA
                     ITLLVAHQLVADIELYRERAEIITVPPLCPLSVSPYDFSQAGDMIERASEQTGRWLDK
                     GGLSRERVPGALRGHTH"
     gene            complement(5977..6951)
                     /locus_tag="VFV55_04680"
     CDS             complement(5977..6951)
                     /locus_tag="VFV55_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4943624.1"
                     /translation="MNKAIPVVSAVIACAAVLAPPAAAQAWPAKAVKFVVPQAPGGAT
                     DVFARYMGQKLSVRWGQPVVVENKAGAAGVVGTDAVAKAAADGYTFLVTYAGSQAVNQ
                     SLYARLPFDSVKDFQPVATLATIPFLLVVGPDSPHKTFRELIAAAKAKPGSVTYATSG
                     SGSVNHLLSESLKVEAGIDMVHIPYKAISAAMTDVMGGQVDNAFASVPSAIQLVRGGK
                     LRALAVSSAKRNSSAPEVPTIAEMGYPSFDVSPWWGILAPAGTPRAIVDKVNADVTEV
                     LKAPDVQAFFKDQGAEPLLTTPDSFLRLLEDDVKKWAKIVKSSGARID"
     gene            complement(6980..7681)
                     /locus_tag="VFV55_04685"
     CDS             complement(6980..7681)
                     /locus_tag="VFV55_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX4943625.1"
                     /translation="MLRSKVYLKVDRVDARTCALARTTSVADLHESLGGVAGRIGTMS
                     PAMRPLIAGLRIAGPAVTAYCSPGDNLMMHRALYLAHEGDVLVVQAPDSGAQWGDMAA
                     LYAKVKGLEGIVVDGHIRDTNELVELRSPVWATLIGPSSPRKSGHGLVNAPIVCAGVR
                     VEPGDLVVADGDGVIVVPRAEAPAAVARARARMEREEALRPEITAGKHPWFLHGCEEN
                     YRNLDVEEIDAPWHS"
     gene            7680..7832
                     /locus_tag="VFV55_04690"
     CDS             7680..7832
                     /locus_tag="VFV55_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943626.1"
                     /translation="MGFTVEGKGAFFHLTMMAGKAPLPAIAGTRCGAYHDASAVSSVF
                     LAEFDR"
     gene            7829..8572
                     /locus_tag="VFV55_04695"
     CDS             7829..8572
                     /locus_tag="VFV55_04695"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX4943627.1"
                     /translation="MRRNSFSAGYCLAIVLAAVAPPPCLADDGHDSPRRQMVEQIVAI
                     ARDAGADTGKAAIDERVLAAMSRVPRHRFVPPDQERNAYANRPLPIGHGQTISQPYIV
                     ALMTDLLEVKPDHAVLEIGTGSGYQAAILATLARTVCTIEIIAPLAREAEERLGRQGF
                     AQVRTRVGDGYYGWEECGPFDGIIVTAAAGQVPPPLVRQLKPGGRMVIPVGAPFLTQQ
                     LMLVEKNRDGTILTRQILPVRFVPLTGAH"
     gene            8569..11010
                     /locus_tag="VFV55_04700"
     CDS             8569..11010
                     /locus_tag="VFV55_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX4943628.1"
                     /translation="MTIQRPPLLAISLLSAAALGYEVLLMRLFSIIQWHHFAYMAISV
                     AMLGYGTAGTFVALSQRTLQAHFGPVFVGAAASFGFTAVAGFLLAQQVAFNPLEILWD
                     ARQPLRLLAVFGLLFVPFFCAAIAICLTFTRFAGQPHRIYSFDILGAGAGCLGIVAVL
                     FATTPSGALQAIGALGFVAAAVAAYTGAACARALPAVLLGAAVITAIGLPGDWTRLRP
                     SPYKELSQALAIGGTRIVAERSSPMGLVTVAESPQVPFRLAPGLSLNATMEPPPQLGV
                     FIDGDGPGALVHDDGRRESLAYLDSLTSALPYHLLDRPRVLVLGAGAGVDVLQAKYHR
                     ARAIDAVEIDPQVVDLVQNEFGAFSGRPYSAPGVRVHVAEARGFVAGRGGRYDLIQVA
                     LLDAFGATSAGLHSLSENYLYTVEALQEYVDHLDPGGMLAITRWVQLPPRDVLKLFAT
                     AVESLERRGNAQAGRSLALIRGWRTATLLVKNGAFTDAEITALRAFCRARSFDVEYHP
                     GIANAAAAERFNILEQPWFREGTLALLGEGRAAFVERYKFNIVPATDDKPYFFHFFKW
                     RTSPELLAQWGRGGLPLLEWGYPVLVATLVQAVAASLLMILLPLWIVRRRGDPRAGPP
                     ESHVREAFYFLAIGFAFMFVEMAFIQKFLLFLSHPLHAVAVVLSAFLAFAGLGSSYSG
                     RVQPPAAGSRDLRIAWPVAAIIVVGSLYLAILPGLFRYLMGLHDAGKILVSMGLIAPL
                     AFAMGMPFPLALAGLARRCPAFIPWAWGINAFASVVGAILASLLAIHVGFGAVVLLAL
                     LLYGLAWWSSAIIAR"
     gene            11039..>11932
                     /locus_tag="VFV55_04705"
     CDS             11039..>11932
                     /locus_tag="VFV55_04705"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEX4943629.1"
                     /translation="MNEAAAQVVLLVRAVEGADPAGKALTKDDRGYAARAAAELVRWQ
                     AADQGGRATEEAFIVKRAELLAARLAERSPNIHRALGAMRWRPWIGIALPVLAFAIGI
                     AAEHIADRRHVNILAFPLLGLVVWNIAVYVALIVRGATNLASRSRREPGWIESRLAGA
                     RGALADRAAGPLSGALAGFAFAWAQHSAPLVAARAARILHVSAAMLAIGAVAGLYVRG
                     MAFEYRAGWESTFLDANSVHAILTFFLQPAAWLTGQPLPSAGGIAALRWGGNGTGENA
                     ARWIHLYTATVMLSVVIPRLVL"
BASE COUNT         1722 a         4046 c         4201 g         1963 t
ORIGIN      
        1 gatgcaggtg cgaccaacgg ccaggtcacc cttgtcgggc tggcgcagcg cggcgagaag
       61 gcggccgcga tcgagcgcgc ctcggcgacg atcaagggga cgcgaagctc gcacgaaatc
      121 cgcgtggagg cggattttcc ggacaagtcc acggcgcggg cgcttttcga cggcggcatc
      181 gtggcgggcg cccgcctgcc ggagtggcgc gggcgcctcg aatccctgga gacggcgggg
      241 cgggccagct tcgccctcgc ggccccggct gcgcttctcg tgtccgcgga tcgcatcgag
      301 ctgggcgagg ccctcttcgc cggcgaaccg ggcgaggtgc gcctcgccgt cacgcgctgg
      361 accccggcgg gcgtggagtc gcgcggctcc tcgagcgccc tggtgatcca gtccattcgc
      421 cggatcctga acctgcaggg ccaggtgggc tcgaacctcg tgctcgcggg caactgggac
      481 ttgcgcgtcg gcgaatccgt ggacggcttc gtttcgctgc gccgcgagcg cggtgacctg
      541 cgcatgggcg aaccgcggca ggcgctgggg ctcgaggtgc tggcgctgcg cgccgatgcg
      601 agcggcgggc gcgtcagggt gacggcagac gtgcgcggaa agcaggcggg ccagtggacc
      661 ggcgagttgt ccgtgatgct gcgcaacggg agcgaaggct gggagatttc tcccgtcgcg
      721 cccatcgagg gccgcttctc cgtggacgtg ccggacctcg cgtggatcgc ggcatggatc
      781 ggcccggagg cgcgcgcggg cggccgcctc aagggcgagg ccacgcttgc cggcaccgcg
      841 cgcgagcccg cctggagcgg gcgcgtcgag gcgaacggcc tcgtcctgcg cgagcctgcc
      901 ctgggcgccg aggtggccga cgggacgatc gcgatcgtgc tcagggatcg cgaggcgcgc
      961 atcgagcgct tcgcgctttc catgccctgg cagccgccgg acgaggccgc tcgcgcgatc
     1021 gccgcggcaa ggcgtcccgc gacgggaacg gtgaccgccg agggcgcggt ggacctcggc
     1081 acgcgcaagg gaacgattca cctgaaggcc gttgcctggc cgctcacgcg cctggcgacg
     1141 cgctttctcg ccgtctcggg cgaggggcgc gcggaactcg acggaaacgt gacggtgatg
     1201 accggcgcat tcaccgcgga tgccggctgg ttcggcatcc cggcctcggc gccgccatcg
     1261 ctttccgacg acgtgatcgt cgaccgcgga ggccaggcac ccgtcgtcgc caagggtgcg
     1321 gagcgcatcc gcctcgacct gcgcgtggac ctgggcgagc acctgcattt ccggggacgc
     1381 gggctggcca cgcggctggc gggatcgttg cggctggcgg gcgacgtcgg cgcgaacctg
     1441 cgcaccacgg gcaccatcca ggcgatgggc ggcacctacg acgcctacgg ccgctcgctc
     1501 gccatcgagc gcggcgcgct caatttccag ggacccgtgg acaaccccgg catcaacgtg
     1561 ctggcgttgc gcaaggggct tccggtagag gcgggcgtcg aggtgctggg cacggtggcg
     1621 cggcccaggg tgcggctcgt ctcgtcgccc gacgtgcctg atcccgagaa gctcacctgg
     1681 ctggtgctgg ggcggggaca gggcgacgta tcggcgggcg acgcggccgt gctggtgggg
     1741 gccgccaacg cgctcctcgg ccgcgacatg ccggccagcg cgaagctcct cggcggcctc
     1801 ggcctcgacg acgtgcaact gggccgcgac tcttccggga ccctcggcac gctgccgcag
     1861 agcacggtgg cgggaaggac gggagcggca tcggcggcgg aggtggtgac ggtgggaagg
     1921 cgcctcaccg acgacatcta cgtctcctac cgccagggcc tcgcggatgc ggaaggatcg
     1981 ctgcgcgtgg cctggcagct cacgcgcagc ctgcagatca tcctgcgcgc cggccacctg
     2041 cccggcatcg acgcggtgta ccgcttctcg ttcgactgaa actacatcgc gtcgagagcg
     2101 gccaccacct tgtcgcgcgc ctcgcgggcg agcggctgga tgtcctgccg cccggtgagg
     2161 gtgaccatcg tctcgggatc gagcaccgac accaccgtct tgccgtccgc gggattctcg
     2221 tagaccgtca cgttgcacgg cagcagcagc ccgatgtcga tctcgccgga gagcgcctgg
     2281 aaggcgagat tgggattgca ggcacccagg atcacgtact tggtgaagtc cttgtccagc
     2341 ttcttcttga gcgtggcctt cacgtcgatc tcggtaagga tcccgaagcc ctgctccttg
     2401 agcgtggcgg tcacgcgctc cacagcctgc tcgtaggtca gcgcgacgat gcgcgagtat
     2461 ccgtacttgc ccttccttgc attcgacatg tgatttgccc ccggaagcga ttgataagga
     2521 aagcctgatt ttactcggtg gccggaccgg gagggcccgt cgtcgggaaa ccgggaccgc
     2581 tccgggcaag acgtcatctg ggggtgcttg acgccggcaa tatttcgatt atcatagaaa
     2641 tacagaaaga gaccaatccc gaaggaaatg cgcatggccg caaggcaacc cgttgaagac
     2701 gtttcgcgct atgccgacat gctctccgcg atgggaaccg agccgcggct gcgcatcatg
     2761 cgcctgctgc tgtcggcgca tccggagggg ctcgtggtgg gggacgtcgg ggcggagctc
     2821 gggatcgcca actcgacgct ctcgcatcac ctcgagaggc tcaggaacga gggcctcgtg
     2881 aacgtccacc gcgagggaac cttcctccgg tactcggcca acgccggggc gctgcaggac
     2941 ctgctccggt tcctgtatgc ggagtgctgc acgcgcagcg gtgcgtgcga accgaagaag
     3001 atcgtttccg ccaactgacg aaaggaagat ccatgtccga accgaacgac gtgaaggaag
     3061 ccgtccgcga gagatacagc caggccgccc tgcgcgtgaa ggggggcggg ggcggttgct
     3121 gcgcgccggc gccggccgcc acgggctgct gtggcagcgc gcccgccgcc acgagctgct
     3181 gtggcaccgc cagagccgcg acgagctcgg acgacccgat cacctccgat ctctacgatg
     3241 cggcgcaggc cggcgccgtg cccgaggcgg cgctgcaggc ttcgctgggc tgcggcaacc
     3301 cgaccgcgct ggccgagctg aaacccggag agacagtgct cgacctgggc tccggcggcg
     3361 gcatcgacgt gctgctctcc gcgcgccgcg tgggtcccac gggcaaggcc tacggactgg
     3421 acatgaccga cgagatgctc gcgctcgccg aggagaacaa gcgcaggagc gggctcgcca
     3481 acgtcgagtt cctgaaggga gagatcgaga gcatcccgct tccggacaac acggtcgacg
     3541 tgatcatctc caactgcgtg atcaacctct ccggcgacaa ggaccgcgtg ctgcgcgaag
     3601 ctttccgggt gctcaagccg ggcgggcgct tcgcggtctc cgacgtggtc gtgcgcggcg
     3661 aggtccccgc ggccatccgc aagagcctgg agctgtgggc cggctgcatt gccggcgcgc
     3721 tctccgaaac cgactacgtg cgcaagctct ccgacgcggg gttcaccgac atcggcgtgg
     3781 aggtcacgcg cgtctacggg ggcgcggatg cacaggagtt cctcgcggcc gggggcttcg
     3841 atgcccaggc catcgccaag gaagtcgacg gcaagttcgt gagcgctttc gtgcgcgccc
     3901 gcaagccgta accattttgg ggacggttcc ggagaaccgt ccccaatacc tgtcccggtg
     3961 accgtctggt ggggcgtaat ggcgccggcc gccacgctcg gggaagtgat cgcacgccgg
     4021 gcgtgggcgg tcaaggactc cggggcggca caggaccagg ccgcttcagg aagcgcgcgg
     4081 gaacgcgatg aagctcccgt ccatccaatg tcgtgaagcc aacggacagg acggtgagga
     4141 caggacatgt ttgcgacaac catcggagag gcaagcctcg atcgcgagcg gcgcgtatcc
     4201 gcctatgccg tcgaagccga aaatttcgag tcgatccaca accattcggc gttcgcccgg
     4261 ctgcgccggc ctggagagta cgcgacatga cgatccacga cgtgacgctc gccgcccgcg
     4321 aggaggtggc cgaaggcacg atggccttcc atttcgccaa gccggcaggt ttcgtcttca
     4381 agccgggcca ggcgatcgac ctcatcctgc ccggccttcc tgacgccaac gggcaaccca
     4441 gccgccacgc gttctcgatc gtcagcgccc ccttcgagcg cgacctggtc gttgccacgc
     4501 gcatgcgcga cagcgcgttc aagcgcgcgc tcggcgccat gccgatcggc gccccggtcc
     4561 aggtcgatgg cccgttcgga tcgctcaccc tgcacaacga ccgcgcccgg gccgcggtgt
     4621 tcatcgcggg cggcatcggc atcacgccgt tcatgagcat gctgaggcac gccgccgccg
     4681 atccgatacc gcgcagcctt acgctgctct attcgaaccg ccggcccgag gacgccgcat
     4741 tcctcgccga gctccagcaa ctggaaaagc gcaacaggaa cttccgcctg gtcgcgacca
     4801 tgacggcgat ggccaggtcc ggccgcccgt gggaaggcga ggtccggttg atcgacgcgg
     4861 acctggtgcg gaaggtcagt gcgggactca ccaccccggt ctactatctc gttggcccgc
     4921 cggcactggt cgagggcttg cgaaaggcgt tgggcgaggc gcagatcgac gacgacgacg
     4981 tgcgcagcga agaattctat ggatactgac ctcgccctcg agaccatgaa tgaacgcgga
     5041 gagcggcacc gcacgggctg accactcgcc cacggccttc gtcctcgccg gaggcgggag
     5101 cttcggcgcc gtgcaggtcg ggatgctgcg tgcgctcacc gcccatgggg tcgtgcccga
     5161 cctggtggtc ggttcctcgg tgggggcgat caacggcgcc ttctttgccg gtgcgcccgg
     5221 ctcggagggc gttgcgcagc tcgaggcgat ctggtgcggg ttgcaccgac aggaagtctt
     5281 cccgttcacc tggcgttgtc tcgtggggtt gttgaggcga cgcaatttcg tggtcgatcc
     5341 cggcggtttg cgcggcgttc tcgagcggta cctgcaattc cgcgaactcg agcatgccgc
     5401 cctgccgctg catgtggtgg cgaccgacca gctcggcggc ggcaccgttc ggctctcgag
     5461 cggccccgcg gtcgaggcgg ttctcgcgag ctgcgcgatt ccggcggcgt ttccaccagt
     5521 tttcatcggc gagcgctacc tgatcgatgg cgcggtggcc agcaacacgc cgatcggcgt
     5581 ggcggtcgag ctgggggcga agcgaatcgt ggtgctgccg acgggcttcg cctgcgcgct
     5641 cgaagcgccg ccgcgcggcg tgatcgccag cgccctgcac gcgatcacgc tgctggtcgc
     5701 gcatcaattg gtggcggaca tcgagctcta ccgggaaagg gcggagatca tcaccgtgcc
     5761 gcctttgtgt ccgctgtcag tgtcgccgta cgatttttca caggccgggg acatgatcga
     5821 gcgggcgtcg gaacagaccg gccgatggct cgacaagggc gggctttcgc gcgagcgcgt
     5881 tcccggcgcg cttcgcgggc atacccattg accccttcgc tctgtacccg gctgtacccg
     5941 gtacatgtac cgggtacatg taccgggtac acgtgctcag tcgatgcgcg cgccggagct
     6001 cttcacgatc ttggcccact tcttcacgtc gtcctcgagc agccggagga atgagtccgg
     6061 cgtggtgagc aggggctccg cgccctggtc cttgaagaag gcctgcacat cgggggcttt
     6121 caggacctcc gtcacgtcgg cgttcacctt gtccacgatg gcccggggcg tgccggccgg
     6181 cgcgaggatg ccccaccacg ggctcacgtc gaagctcggg taacccatct ccgcgatcgt
     6241 ggggacctcg ggcgcactgg aattgcgctt ggcgctggag accgccagcg cgcgcagctt
     6301 gccgccacgc accagctgga tcgccgaagg cacggacgcg aacgcgttgt cgacctgccc
     6361 gcccatcacg tccgtcatgg cggccgagat ggccttgtac gggatatgca ccatgtcgat
     6421 gccggcttcg accttgaggg actccgagag caggtggttc acggagccgc ttcccgatgt
     6481 cgcgtaggtg acggaaccgg gcttcgcctt tgccgcggcg atcagctccc ggaacgtctt
     6541 gtgcggcgaa tcgggcccca ccacgagaag gaacggtatc gtcgccagcg tggccacagg
     6601 ctggaaatcc ttgaccgaat cgaagggcag ccgggcgtag aggctctggt tcaccgcctg
     6661 cgacccggcg taggtgacga gaaaggtgta gccgtccgcc gcggccttgg ccaccgcatc
     6721 ggtgcccacc acgcccgccg ccccggcctt gttctcgacg accaccggct ggccccaccg
     6781 gacgctcagc ttctgcccca tgtagcgggc gaagacgtcg gtggcgccgc ccggcgcctg
     6841 cggcaccacg aacttcaccg ccttcgcggg ccaggcctgc gccgcggccg ggggggcgag
     6901 gacggccgcg caggcaatga cggcggatac gacgggaatg gccttgttca tgggggttcc
     6961 gtggcggatg gcggactgtt caggaatgcc agggggcatc gatttcctcg acgtccaggt
     7021 tgcggtagtt ctcctcgcag ccgtgcagga accacgggtg cttgcccgcc gtgatctccg
     7081 ggcggagcgc ctcctcgcgt tccatgcggg cacgggcgcg cgcgacggcg gcgggtgcct
     7141 ctgccctggg gaccacgatc acgccatcgc catcggcgac gacgaggtcg cccggctcga
     7201 cgcgcacgcc ggcacagacg atcggcgcgt tcaccagccc gtggccggac ttccgcggac
     7261 tgctcggccc gatgagggtc gcccacacgg gcgagcgaag ctccacgagc tcgttggtgt
     7321 cgcggatgtg accatcgacc acgatgccct cgagaccctt caccttcgcg tacagggccg
     7381 ccatgtcgcc ccactgcgcg ccggaatcgg gcgcctgcac gaccagcacg tcgccttcat
     7441 gcgcgagata gagcgcgcgg tgcatcatca ggttgtcgcc gggcgagcag tacgcggtga
     7501 ccgcggggcc ggcgatgcgc agtcccgcga tcaacgggcg catggcgggc gacatcgtgc
     7561 cgatgcggcc tgccacgccc cccaacgatt catgcaggtc ggcgacgctc gtggtgcgcg
     7621 ccagggcgca ggtccgcgcg tccacgcggt cgaccttcag gtagaccttc gagcgaagca
     7681 tgggattcac cgttgaaggc aagggcgcat tctttcacct gacgatgatg gcgggcaagg
     7741 caccgctgcc ggcaattgcc ggtacccggt gcggcgccta tcatgatgct tccgcggttt
     7801 cctccgtgtt ccttgcggaa ttcgaccggt gaggcgcaac tccttcagtg ccgggtactg
     7861 cctcgccatc gtgcttgcgg cagtcgcgcc gccgccatgc ctcgccgacg acggtcacga
     7921 ttcgcctcga cggcagatgg tcgagcagat cgtggccatc gcgcgggacg cgggcgcgga
     7981 tacgggcaag gcggctatcg atgagcgcgt cctggccgcg atgagccgcg tgccgcggca
     8041 ccggttcgtc ccgccggacc aggagcgcaa cgcctacgcg aaccggccgc tgcccatcgg
     8101 gcatggccag acgatctccc agccgtacat cgtggccctg atgaccgacc tgctcgaggt
     8161 gaagccggat cacgccgtgc tcgagatcgg aacgggttcc ggataccagg cggcgatcct
     8221 ggcaacgctg gcccggaccg tatgcacgat cgagatcatc gcgccgctgg cacgcgaggc
     8281 ggaagaacgg ctgggcaggc aggggttcgc gcaggtgcgc acgagggtgg gcgacggtta
     8341 ctacggctgg gaggagtgcg ggccgttcga cggcatcatc gtcaccgccg cggcggggca
     8401 ggttccgccg cccctcgtcc ggcagctcaa gccgggcggg cgcatggtga tcccggtcgg
     8461 agccccgttc ctgacgcagc agctgatgct ggtggagaag aatcgtgacg ggacgatact
     8521 cacccgccag atcctgccgg tgcggttcgt gccgctgacc ggggcgcatt gaccatccag
     8581 cgtccaccgc tcctcgccat ctcgctgctg tccgcggcgg cgctcgggta cgaagtgctg
     8641 ctgatgcgcc tgttttccat catccagtgg catcacttcg cgtacatggc gatcagcgtg
     8701 gccatgctgg gctatgggac ggcgggaacc ttcgttgcgt tgtcgcagcg caccttgcag
     8761 gcgcatttcg ggccggtctt cgtgggtgcg gccgcatcgt tcggctttac cgcggtggcc
     8821 ggctttctgc tcgcccagca ggttgccttc aatccgctgg agatcctgtg ggacgcgcgg
     8881 cagcccttgc ggctgctcgc ggtcttcggg ctgctgttcg tgccgttctt ctgcgccgcg
     8941 attgcgatct gcctcacgtt cacccgcttc gccggccagc cgcaccgcat ctacagcttc
     9001 gacattctcg gcgccggcgc cggatgcctg ggaatagtgg cggtgctgtt tgccaccacg
     9061 ccgtcgggcg cgctccaggc gatcggcgca ctcgggttcg tcgcggccgc ggtggcagcc
     9121 tataccggcg cagcgtgcgc gcgtgcgctc ccggccgtgc tgctcggtgc cgccgtcatc
     9181 accgcgatcg gactgcccgg cgactggacg cgcctgcgcc cttccccgta caaggagttg
     9241 agccaggcac tggcgatcgg cggcacgcgg atcgtcgccg aacgctccag cccgatgggc
     9301 ctcgtgaccg tggccgagag cccgcaggtt ccctttcggc tcgcgcccgg gctgagtctc
     9361 aatgccacga tggagccgcc gccgcagctg ggcgtcttca tcgacggcga cgggccgggc
     9421 gcgctggtgc atgacgacgg gcgacgcgag tcgctcgcgt acctcgacag cctcacctcc
     9481 gcgctgccct atcacctgct ggatcgcccg cgcgtgctgg tgctcggcgc cggggcgggt
     9541 gtcgacgtgc tgcaggcgaa gtatcaccgc gcccgggcga tcgacgcggt cgagatcgat
     9601 ccgcaggtgg tggacctggt gcagaacgag ttcggcgcgt tttccgggcg accgtacagc
     9661 gcgcccggcg tgcgcgttca tgttgctgag gcgcgcggct tcgtggcggg ccgcgggggc
     9721 cgttacgacc tcatccaggt ggcgttgctg gatgccttcg gggcgacgtc cgcgggactg
     9781 cattcgcttt ccgaaaacta cctgtacacc gtcgaggcgc tgcaggaata cgtggatcat
     9841 ctcgaccccg gaggcatgct cgcgatcacg cgctgggtgc agctgccgcc gcgcgacgtg
     9901 ctgaagctct tcgccacggc ggtcgagtcg ctggaacggc gcggtaacgc gcaggcggga
     9961 cggagcctcg cgctgatccg cggctggcgg acggcgacgc tgctggtgaa gaacggcgcc
    10021 ttcaccgacg cggagatcac ggcgctgcgg gcattctgcc gcgcccgctc cttcgatgtc
    10081 gagtaccacc cgggcatcgc gaatgccgcg gcagccgaac gcttcaacat cctcgagcaa
    10141 ccgtggttcc gcgagggcac gctggccttg ctgggcgagg ggcgcgcggc gttcgtcgag
    10201 cgctacaagt tcaacatcgt gccggcgacc gacgacaagc cctatttctt ccacttcttc
    10261 aaatggcgca cgtcgcccga gctcctggcg cagtggggaa ggggcggact gccgctgctg
    10321 gagtggggct acccggtgct ggtcgcgaca cttgtccagg cggtcgccgc gagcctgctc
    10381 atgatcctgc tgccgctgtg gatcgttcgg cgccgcggcg acccgcgcgc cggcccgccg
    10441 gaatcgcacg tgcgcgaggc gttctacttc ctcgccatcg gcttcgcgtt catgttcgtc
    10501 gagatggcgt tcatccagaa attcctgctg ttcctgagcc atccactcca tgccgtggcg
    10561 gtggtcctga gcgcctttct cgcctttgcc gggctgggca gcagctactc cgggcgcgtg
    10621 cagcctccgg ccgccggctc gcgcgacctg cggattgcat ggcccgtcgc cgcgatcatc
    10681 gtcgtcgggt cgctctacct ggccattctt cccggcctgt tccggtacct gatgggcttg
    10741 cacgacgcgg ggaagatcct cgtctcgatg gggctcatcg cgccgctcgc gtttgccatg
    10801 gggatgccct tcccgctcgc ccttgcaggt ctcgcccgcc gctgcccggc gttcatcccc
    10861 tgggcctggg gaatcaacgc gttcgcgtcg gtggtcggcg cgattctcgc cagcctgctg
    10921 gcgattcacg tggggttcgg cgcggttgtc ctgctcgcgt tgctgcttta tggcctggcg
    10981 tggtggtcgt cggccataat cgcccgatga cgatgccgga tgtcaccgga ggcatggcgt
    11041 gaacgaagcg gccgcacagg tggtgctgct cgtccgcgcg gtcgagggcg cggacccggc
    11101 gggcaaggcg ctcacgaagg atgatcgcgg ttacgccgcg cgcgccgcgg ccgagctcgt
    11161 tcgctggcag gcggcggacc agggcgggcg tgcgaccgag gaggcgttca tcgtcaagcg
    11221 cgcggagttg ctcgccgcca ggctcgccga gcgctcgccc aacatccacc gcgcactcgg
    11281 ggcgatgcgc tggcgcccct ggatcggcat tgctctgccg gtcctggcat tcgcgatcgg
    11341 catcgcggcg gagcacatcg cggacaggcg acacgtgaac atcctcgcgt ttccgttgct
    11401 cggactcgtc gtgtggaaca tcgccgtcta cgtcgcgctc atcgtccggg gagcaacgaa
    11461 cctggcttcg cgttcccgcc gcgagccggg ctggatcgaa agccgtcttg cgggcgcccg
    11521 cggcgcactt gccgatcgcg cggcgggccc gctttcgggg gcgctggcag gcttcgcatt
    11581 cgcctgggcg cagcactccg cgcccctcgt cgccgcccgg gcggcgcgga tcctgcacgt
    11641 gtccgccgcc atgctcgcca tcggcgcggt tgcagggctg tacgtgcgcg gcatggcgtt
    11701 cgagtatcgc gccggctggg agagcacgtt tctcgacgcc aattccgtgc acgcgatcct
    11761 gacgttcttc cttcaacccg ccgcatggct gaccggccag ccattgccgt cggccggcgg
    11821 aatcgccgca ttgcgttggg gcggcaatgg cacgggagaa aacgccgctc gctggatcca
    11881 tctctacacc gcgaccgtca tgctgtcggt ggtcatcccc cggctggtcc tc
//
LOCUS       DASUEV010000144         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_256877, whole genome shotgun sequence.
ACCESSION   DASUEV010000144 DASUEV010000000
VERSION     DASUEV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256877"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1410
                     /locus_tag="VFV55_04710"
     CDS             130..1410
                     /locus_tag="VFV55_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4943630.1"
                     /translation="MEVIGKYQIVRELGRGATGKVYLATDPFGNRQVAIKVAFPEALK
                     SSEDGAFYRNMFLNEAALAGKLNHPHIVHIYDAVVEGEFSYIVMEYVEGGTLEKFCES
                     GALLDPAEVAEIAFKCVRALAFAQRLGLTHRDIKPGNILHVDGTDIKIADFGAAINTV
                     SDRTVIANVGSPAYMAPELITGTAQATALTDIYALGVVMYYLLAGRLPFAGANTMSVI
                     YQIVNTEPEAPSVHREGLSADLDAIVLKAMAKDPARRYATWEEFGQALAEVWKKTQRP
                     GDREQSDTERFNLCRNLPFFKEFPENELWEVLRLSKWRKFPPNSVLIKEGDQGDSFFI
                     LAGGYVRVTRGKRTLSVLSAGDCFGEMSYLAHKDAPHRSASVTTTSDCIVVKVRAEDL
                     RASSLSCRRLFDERFLNTLVERLEAANEQLAVMS"
     gene            complement(1418..1906)
                     /locus_tag="VFV55_04715"
     CDS             complement(1418..1906)
                     /locus_tag="VFV55_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HEX4943631.1"
                     /translation="MDPLYFHIVAVVLVIVGLAGTVLPLLPGVPLVFGGLLVAAWADG
                     FQRVGAITLVVLGVLTALAVAADLLASALGAKRMGASPRAVAGATVGAVIGIFFGLPG
                     LVLGPFVGAVAGELSANRQLLHAGRAGLGTWIGLLLGSIAKLTLAFLMVGVFATAWLL
                     AG"
     gene            2009..3496
                     /locus_tag="VFV55_04720"
     CDS             2009..3496
                     /locus_tag="VFV55_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4943632.1"
                     /translation="MASTAIPAENTQRRAAVSQALSARLPAHAVLTAREDLLPYECDG
                     LSAYRQTPLVAALPGTVEEAAEVLRVARETGTPVVARGSGTGLSGGALPLADGILLSM
                     GKFNRILAIDPLARVAHAQPGVRNAQITEAAAPLGLYYAPDPSSQIACSIGGNVAENS
                     GGVHCLKYGLTVHNIRKVRALTIEGDAIELGSDALDAPGYDLLAVITGSEGLLALLTE
                     VTVMLRPKPQVAQVVMASFTSVEEAGEAVAAVIGAGIVPAGLEMMDRMATAAVEDYVH
                     AGYDLNAEAILLCESDGTPEEVAEEIAKITEVMKGAGSTAVAVSKSEAERLRFWSGRK
                     AAFPAVGRISPDYYCIDGSIPRKALARVLRRIEELSKHYGLRCANVFHAGDGNLHPLI
                     LYDANIPGELEKTEEYAGEILELCIEVGGTITGEHGVGVEKIRQMCVQFRPAELAQFH
                     AVKAAFDPPGLLNPGKAVPTLNRCADYGALRVSRGQLPHPDLPRF"
     gene            3496..>4003
                     /locus_tag="VFV55_04725"
     CDS             3496..>4003
                     /locus_tag="VFV55_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4943633.1"
                     /translation="MGAAAVRSDRRAEVLAVLAAVRPPVSVVAERERLAVFEMDAYIQ
                     KRETPLAAVLPDTEEQVRDIVVSLGRLGVPIVSRGAGTGISGGATPTADGVMLVLTRM
                     RRIVSIDAAARLATVEPGVANAQVTVAAEPASLFFAPDPSSQIISSIGGNIAENAGGI
                     HCLKYGLTV"
BASE COUNT          678 a         1375 c         1360 g          590 t
ORIGIN      
        1 agtacgccga tagacttgcc ccatcgccgg cctgacttcc attcgccgcg gacgtcgggc
       61 gcccgcgtcg cgcccgttcg aacgggctcg aaaagggacc cgctcccttt ttccctataa
      121 tcccgttata tggaagtgat cgggaaatac cagatcgtcc gcgagctggg caggggagcg
      181 acggggaagg tctatctggc cacggacccg ttcggcaatc gccaggtcgc gatcaaggtc
      241 gcgtttccgg aggcgctcaa gagcagcgag gacggcgcgt tctacaggaa catgttcctc
      301 aacgaggcgg cgctcgccgg caagctgaac catccgcaca tcgtccatat ctacgatgcg
      361 gtggtggagg gcgagttcag ctacatcgtg atggagtacg tcgagggcgg cacgctcgag
      421 aagttctgcg agtccggcgc actcctcgac ccggccgagg tggccgagat cgccttcaag
      481 tgcgtccggg ctctcgcctt cgcgcagcgg ctgggcctca cgcaccgcga catcaagccg
      541 ggcaacatcc ttcacgtcga cggcaccgac atcaagatcg ccgacttcgg cgccgcgatc
      601 aacacggtct cggaccgcac cgtgatcgcc aacgtgggct cccccgcgta catggcgccg
      661 gaactgatca cgggcaccgc gcaggccacg gcgctcacgg acatctacgc gctcggcgtg
      721 gtcatgtatt acctcctcgc ggggcgcctg ccgttcgccg gcgccaacac gatgagcgtc
      781 atctaccaga tcgtgaatac ggaacccgag gcgcccagcg tccatcgcga ggggctgtcc
      841 gcggatctcg acgcgatcgt cctgaaggcg atggccaagg atccggccag gcgatatgcc
      901 acgtgggagg agttcggcca ggcgctggcg gaggtctgga agaagacgca gcggccggga
      961 gatcgcgaac agtccgacac cgagcgcttc aacctgtgcc gcaacctccc cttcttcaag
     1021 gagttcccgg agaacgaact gtgggaagtg ctgcgcctgt cgaagtggcg caagttcccg
     1081 cccaatagcg tcctcatcaa ggagggtgac cagggcgact cgttcttcat cctcgcgggg
     1141 ggctacgtgc gcgtgacgcg gggcaagcgc acgttgagcg tgctctcggc cggggactgc
     1201 ttcggcgaga tgtcgtacct cgcgcacaag gatgcaccgc accgctcggc ttcggtcacc
     1261 accacgtcgg actgcatcgt ggtgaaggtc cgcgccgagg acctgcgcgc ctcgtcgctg
     1321 tcgtgccggc gcctcttcga cgagcgcttc ctcaacacgc tggtggagcg actcgaggcc
     1381 gcgaacgagc agctcgcggt catgtcctga ggagcgccta gcccgcgagc agccaggcgg
     1441 tggcgaagac gcccaccatc aggaacgcga gcgtgagctt ggctatcgag ccgagcagca
     1501 gcccgatcca tgtccccaga ccggccctgc ccgcgtgcag caactggcga ttggccgaaa
     1561 gctcccccgc gaccgccccc acgaacggtc ccagcaccag ccccggcagc ccgaagaaga
     1621 tgccgatgac cgcgccgacg gtcgcgccgg ccactgcccg cgggctcgct cccatcctct
     1681 tggcgccgag ggcggaagcg agaagatcgg ccgcaaccgc aagcgcggtg aggactccca
     1741 ggaccacgag cgttatcgcg cccacgcgct ggaacccgtc cgcccaggcc gccacaagca
     1801 ggccaccgaa caccaggggc acgccgggca gcaagggcag gacggttccc gccagcccca
     1861 cgatgacgag gacgacggcg acgatgtgga aatagagggg atccacggcg ggcgaaggac
     1921 ctgttaccgg cgctctccgg aaggacacgg cgcccatgct atcgtatgtc cacccccggc
     1981 aaggcaaccc gacccgttca ttgcgaaaat ggcatcgacc gcgattcccg ccgagaacac
     2041 gcaacgccgg gcggcggtgt cgcaggcgtt gtcggcgcgc ttgccggcgc acgccgtgct
     2101 cacggcgcga gaggacctgc tcccctacga gtgcgacggc ctgtcggcct accggcagac
     2161 gcctctcgtg gcggcattgc cgggcaccgt ggaagaggcc gccgaagtgc tgcgcgtggc
     2221 tcgcgaaacg ggcacgccgg tggtcgcgcg cggttcgggg accggtctct cgggaggggc
     2281 gctgcccctt gccgacggca tcctgctgtc gatgggcaag ttcaaccgca tcctcgccat
     2341 cgatccgctg gcccgcgtgg cgcatgcgca gccgggcgtg cgcaacgcgc agatcacgga
     2401 agccgcggcg ccgctgggcc tctactacgc gcccgacccc tccagccaga tcgcctgctc
     2461 catcggcggc aacgtggcgg aaaattccgg cggcgtgcac tgcctcaagt acggcctgac
     2521 ggtgcacaac atccggaagg tgcgcgcgct cacgatcgag ggcgacgcga tcgagctcgg
     2581 ctccgacgcc ctcgatgcgc cgggctacga cctgctcgcg gtgatcaccg gttccgaggg
     2641 cctcctcgcg ctgctcacgg aggtcacggt gatgctgcgt ccgaaaccgc aggtggcgca
     2701 ggtcgtgatg gcctccttca cgagcgtgga ggaagcgggc gaggccgtcg ccgccgttat
     2761 cggtgcgggc atcgtgccgg caggcctgga gatgatggac aggatggcca ccgccgccgt
     2821 ggaggactac gtgcacgcgg gctacgacct gaacgccgag gcgatcctgc tgtgcgaatc
     2881 cgacggcacg cccgaggaag tggccgagga gatcgccaag atcacggaag tcatgaaggg
     2941 cgcgggctcg acggcggtcg cggtctcgaa gagcgaggcc gagaggctcc ggttctggtc
     3001 ggggcgcaag gccgcgttcc ccgccgtggg gcggatctcg ccggattact actgcatcga
     3061 cggctcgatc ccgcgcaagg ctctcgcgag ggtgctgcgc cgcatcgagg aactctcgaa
     3121 gcattacggg cttcgctgcg ccaacgtttt ccacgcgggc gacggcaacc tccaccccct
     3181 catcctctac gatgccaaca ttcccggcga gctggaaaag accgaggaat acgcgggcga
     3241 gatcctcgag ctctgcatcg aggtgggcgg caccatcacc ggcgagcacg gcgtgggcgt
     3301 ggagaagatc cgccagatgt gcgtgcagtt caggccggcg gagctggcgc agttccacgc
     3361 ggtgaaggcc gccttcgacc cgccggggct cctcaatccc ggcaaggccg tgccgacgct
     3421 caatcgctgc gcggactacg gcgccttgcg cgtgagccgc ggccagcttc cgcaccccga
     3481 cctgccgagg ttctgatggg cgccgccgcc gttcgctccg atcgacgcgc cgaggttctc
     3541 gcggtgcttg cggccgtgcg accgccggtg agcgtcgttg ccgaacgcga gcgactggcc
     3601 gtgttcgaga tggacgccta catacagaag cgcgagacgc cgttggcggc cgtgctgccc
     3661 gacaccgagg agcaggtgcg cgacatcgtc gtgtcgctcg ggcgcctggg cgtgcccatc
     3721 gtgtcgcgtg gcgcgggaac gggcatttcc ggcggcgcca cgccgacggc cgatggcgtg
     3781 atgctcgtcc tcacgcgcat gcgtcgcatc gtctccatcg acgcggccgc gcggctcgcg
     3841 acggtggagc cgggcgtggc aaacgcgcag gtgacagtgg cggcggagcc cgcttccctc
     3901 tttttcgcgc cggacccgtc cagccagatc atctcgtcca tcggcggcaa catcgcggag
     3961 aacgccggcg gcatccactg cctcaagtac gggctcaccg tgc
//
LOCUS       DASUEV010000145         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_843457, whole genome shotgun sequence.
ACCESSION   DASUEV010000145 DASUEV010000000
VERSION     DASUEV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843457"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFV55_04730"
     CDS             <1..655
                     /locus_tag="VFV55_04730"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX4943634.1"
                     /translation="VGYPVAEIAADGSCVIGKAAQTGGCVTLATVKEQLLYEVHDPAA
                     YLTPDVTADIRDARLAGAGPDRVALSGVRGHERPATLKVTAFYEGGWLAEGEISYAGP
                     GAEARARLAADILARRLPELRLRVDLIGVVSVFADDAGRLLESTPWGDARDVRLRVAA
                     MHAQREVAERLGEEVTALYTCGPAGGGGVRTAIRQRLSTASCFVPREAVHARFDWVN"
     gene            655..1011
                     /locus_tag="VFV55_04735"
     CDS             655..1011
                     /locus_tag="VFV55_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943635.1"
                     /translation="MPSSTVPLRTLAHGRTGDKGNRSNISVIAYRPESWELLVTQVTE
                     AAVARALAHRAPSRVTRYLLPNLHAMNFVIDEVLDGGVNDSLNLDSHGKALAFLVLDL
                     PVDVPDDLITKTGDYP"
     gene            1008..1961
                     /locus_tag="VFV55_04740"
     CDS             1008..1961
                     /locus_tag="VFV55_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4943636.1"
                     /translation="MKRIIASVLFPLAATVALAQEFPAKTITFVVPFAAGSATDVLAR
                     SVGQAVSAETKQAVVIDNKPGASGMIAAQFVAKAPPDGYTVLITTNTTHAANEHLYKK
                     LSYDPVKDYAPLTGLGKGGQVMVVRNDLPVKSVAEFIAFARKSPGKINFGSGSSSSRI
                     AGELLQQMAGIELLHVPYKSNPLAIADMLGGTIDMMITDTTTGLPQVQAGKLRALGYS
                     GDKRSRLAPDLPTISEAGVKGYEMTYWFAAYAPAGTPPATVKRLNALLVAATKSAPVT
                     AFYEKTGVDAFTTTPEELGRFQLAETKKWGEIIRKANIQPE"
     gene            2010..2756
                     /locus_tag="VFV55_04745"
     CDS             2010..2756
                     /locus_tag="VFV55_04745"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX4943637.1"
                     /translation="MDPSPPSGSIFRARDLLEHATSMAVLTGAGISAESGIPTFRDAL
                     TGLWEKFRPEDLATPEAFEANPKLVWDWYAWRRNLVAKARPNAGHRALVELERKCRER
                     EVDFTLVTQNVDGLHRTAGSNRVIELHGNIGRVKCFDRHHPVHSWPDGDEVPTCPRCG
                     SLLRPDVVWFGEGLPPDALAAAVAAARTCDVFLCVGTSTVVEPAASLPVIAMEAGARV
                     IEVNPQPTPLTLRASVSLRGAAGEILPLLA"
     gene            complement(2839..>3489)
                     /locus_tag="VFV55_04750"
     CDS             complement(2839..>3489)
                     /locus_tag="VFV55_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase"
                     /protein_id="HEX4943638.1"
                     /translation="EDQMWEKGYLDSMQMSGAFQLLRSVDLVWSSLVKQYLKGERTPV
                     NDLMSWNADGTRMPYRMHTEYLRSLFLRNELATGRFKVWGRPIALPDIDIPIFCVGTV
                     TDHVAPWRSVYKLHLLTHAELTFALTTGGHNVGVVNPPSPESKRRYKLRTRPMGSDYL
                     DPEAFEQTADEFAGSWWTAWESWLSRRSTGKVAPPKMGGTSFTPVGDAPGKYVFMR"
BASE COUNT          586 a         1182 c         1149 g          572 t
ORIGIN      
        1 tgtgggctat ccggtggcgg agattgcggc cgatggaagc tgcgtgatcg gcaaggccgc
       61 gcaaaccggc ggttgcgtga cgctcgccac cgtgaaggaa cagctcctct acgaagtgca
      121 cgatccggcg gcgtatctca cgcccgacgt cacggccgat atccgcgatg cacgactcgc
      181 cggggccggg ccggaccgcg tggcgctctc cggcgtgcgc ggccatgagc gtccggctac
      241 cctcaaggtg accgcgttct acgaaggcgg ctggctggcg gagggggaga tttcctacgc
      301 cggaccgggt gctgaggcga gggcgaggct cgcggccgac atccttgccc ggcgcctgcc
      361 ggagctcagg ctgcgtgtcg atctcatcgg cgtggtgagc gtgttcgccg acgacgcggg
      421 gcgcctgctc gaatccacgc cgtggggcga cgcgcgcgac gtgcggttgc gcgtggctgc
      481 catgcacgcg cagcgggagg tggccgaacg cctgggcgag gaagtgaccg cgctctacac
      541 ttgcgggccg gcgggcggcg gcggcgtgcg cactgcgatt cgccagcgac tgtcaaccgc
      601 ttcctgcttc gtgccgcgtg aggcggtgca tgcgcgattc gactgggtga actgatgcct
      661 tccagcaccg ttccgctgcg cacgctcgcc catggccgca ccggcgacaa gggcaaccgc
      721 tccaacatca gcgtgatcgc ctaccgtccc gaatcctggg aattgctcgt cacgcaagtg
      781 accgaggccg ccgtagccag ggccctggcg caccgcgcgc cttcgcgggt gacgcgctac
      841 ctgctgccca acttgcacgc catgaacttc gtgatcgacg aggttctcga cggcggcgtg
      901 aacgattccc tcaacctcga ttcgcacggc aaggcgctcg cgttcctggt gctcgacctg
      961 cccgtggatg tccctgacga cctcataacc aagaccggag attacccatg aagagaatca
     1021 tcgcctccgt actgttcccg ctcgcggcca cggttgcgct ggcacaggag ttccccgcga
     1081 agacgatcac gttcgtggtg cccttcgcgg ccggcagtgc caccgacgtg ctcgcgcgat
     1141 cggtcgggca ggcggtatcc gcggagacga aacaggcggt ggtcatcgac aacaagccgg
     1201 gtgcgagcgg catgatcgcg gcgcagttcg tcgccaaggc gccgccggat ggctacacgg
     1261 tgctcatcac gaccaacacc acgcacgccg ccaacgagca cctctacaag aagctttcct
     1321 acgacccggt aaaggattac gcgccgctca cggggctcgg caagggcggg caggtgatgg
     1381 tggtgcgaaa tgacctgccg gtgaagtcgg tggcggagtt catcgcgttt gcaagaaaga
     1441 gccccgggaa gatcaatttc ggcagcggca gctcgtcgtc gcgcatcgcg ggcgagctcc
     1501 tgcagcagat ggccggcatc gagctcctgc acgtgcccta caagagcaac ccgcttgcaa
     1561 tcgccgacat gctgggcggc accatcgaca tgatgatcac cgatacgacg acggggctgc
     1621 cgcaggtgca ggccggcaag cttcgcgcgc tgggctactc cggcgacaag cgatcgcgcc
     1681 tggccccgga cctgccgacg atttccgaag ccggcgtgaa gggctacgag atgacctact
     1741 ggttcgccgc ctacgcgccc gccggcacgc cgccggccac cgtcaagcgc ctcaacgcgt
     1801 tgctggtggc ggcgacgaaa agcgccccgg tcaccgcctt ctacgaaaag accggcgtcg
     1861 acgccttcac caccacgccc gaggaactcg gccggttcca gctcgccgag acgaagaagt
     1921 ggggcgagat catcaggaaa gccaacatcc agcccgaata gcgggacagt ggattgcgta
     1981 ccgggtacat gtacccggta catgagccca tggacccctc acccccttcc ggatcgattt
     2041 tccgcgcgcg cgacttgctc gagcacgcga cctcgatggc agttctcacc ggcgcgggga
     2101 tttccgcgga atccggcatc cccacatttc gcgatgcgct gaccggcctt tgggagaagt
     2161 tccgtcccga ggatctcgcc acccccgaag ccttcgaggc caacccgaag ctggtgtggg
     2221 attggtatgc atggcggcga aatctcgtcg cgaaggcccg gcccaatgcc ggccatcgcg
     2281 cgctcgtgga actcgagcgg aaatgccgcg agcgcgaggt cgatttcacc ctggtcacgc
     2341 agaacgtgga cggactgcat cgcacggccg ggagcaatcg cgtcatcgag ctccacggca
     2401 atatcggccg cgtgaagtgc ttcgaccgcc atcaccccgt gcactcgtgg ccggatggcg
     2461 atgaggtgcc cacctgtccc cggtgcggct cgctcctgcg ccccgacgtc gtatggttcg
     2521 gcgaaggtct tccgccagac gcgctcgccg cggcggtcgc agccgcacgc acctgcgacg
     2581 tgttcctgtg cgtgggcact tccacggtgg tcgagccggc ggcttccctt cccgtcattg
     2641 cgatggaagc cggcgcgcgc gtcatcgagg tgaatccgca gcccacgccg ctcacgctgc
     2701 gggcgagcgt gagcctgcgc ggcgcggccg gcgaaatcct gccgctgctt gcctgatccg
     2761 cgcggcgcaa aagggctgtc cggatggttc tctgtacccg gtacatgtac cgggtacaga
     2821 gaaccgtccc caaatcggtc agcgcatgaa gacgtacttg cccggcgcgt cgccgaccgg
     2881 cgtgaagctc gtgccaccca tcttcggcgg cgccaccttg ccggtggagc gcctggagag
     2941 ccacgattcc cacgccgtcc accacgagcc ggcgaattcg tcggcggtct gctcgaaggc
     3001 ctccggatcg aggtagtcgc tccccatcgg ccgcgtgcgc agcttgtacc tgcgcttgga
     3061 ttcgggcgaa ggcgggttca cgacgccgac gttgtgcccg cccgtggtga gggcgaacgt
     3121 gagttcggcg tgcgtgagga ggtgcagctt gtacaccgat cgccacggcg ccacgtggtc
     3181 cgtgaccgtg cccacgcaga agatcgggat atcgatgtcg ggcagcgcga tcggccggcc
     3241 ccacaccttg aagcgtcccg tcgcgagctc gttgcgcagg aagaggctgc gcagatattc
     3301 ggtatgcatg cgatacggca ttcgcgtacc gtccgcgttc cacgacatca ggtcgttgac
     3361 gggcgtcctc tcgcccttca ggtactgctt cacgaggctg gaccacacca ggtccacgct
     3421 cctcagcaac tggaacgcgc ccgacatctg catggagtcg aggtagccct tctcccacat
     3481 ctggtcctc
//
LOCUS       DASUEV010000146         8630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1411120, whole genome shotgun sequence.
ACCESSION   DASUEV010000146 DASUEV010000000
VERSION     DASUEV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411120"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFV55_04755"
     CDS             complement(<1..836)
                     /locus_tag="VFV55_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4943639.1"
                     /translation="MGARPRLIALEADAVPADSMPADRDDGLLDAYSQAVVSAVDRVR
                     DAVVLINAFHGQAGRAGTGSGFIFAPGGYLLTNSHVVHGAKRIEMTLADGRRGPASLV
                     GEDPETDLALLHFEARENLPWAPFGDSARLRVGQLAIAIGNPLGYTHTVTTGVVSALG
                     RTLRSTTGRLIHNVIQTDAALNPGNSGGPLLDSNAQVIGVNTAIIAGAQAICFATAID
                     SAKWVVGELFAHGRVRRAYVGLSGATVPINRRTARFLEVEQQSAMRVLRGGEVKFLLV
                     VPG"
     gene            876..1526
                     /locus_tag="VFV55_04760"
     CDS             876..1526
                     /locus_tag="VFV55_04760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4943640.1"
                     /translation="MTSSQGAPPGAPFNDPAGTWNRRFEEDGFHFGTAPNTWLVEHAD
                     LFRPGQRVLCVADGEGRNSVWLAKRGLEVDAFDIAGVGVEKARRFAAEHGVTVNFAVS
                     DCDAYAWQQEHYDGVVAIFVQFADPAMRGRLFANMKRSLRPGGVLVLQGYTPKQLEYR
                     TGGPPIASHLYTVEMLREAFADLKIVELRKYEAQLAEGKGHCGRSALIGLIARRPG"
     gene            1553..1939
                     /locus_tag="VFV55_04765"
     CDS             1553..1939
                     /locus_tag="VFV55_04765"
                     /inference="COORDINATES: protein motif:HMM:NF025154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HEX4943641.1"
                     /translation="MDSLLTVMPPSWLVRAAVAGVWIYEGLWCKILGRSDNELRVVKA
                     VPRFGERFGPPFLMALGWVELGLGVWVLSGWTPGLCAAAQTLLLVALNANGLLWSRQH
                     IHDPAGMVFKNFAFLILAWVNAGLAA"
     gene            1936..2841
                     /locus_tag="VFV55_04770"
     CDS             1936..2841
                     /locus_tag="VFV55_04770"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HEX4943642.1"
                     /translation="MTGDAPGTAWEAGRLDAGHGPPRVLFGRMYEDPAIELSAFRPGG
                     RVFCIASAGCTALALAPSHPVVAVDINPAQLTYAARRIAGEPMVRGTAESLMAFGRTF
                     LPLAGWRRSTLATFLDLDDPGRQREFWKAHLDTLRFRAALKVLLSFTALRAGYAPGLL
                     SALPPRFDEVMRRRLERCFSTHPNRSNLWARALLLGELARETPSTQAMAIELVRADAA
                     TFLESAPARSFDGFTLSNILDGATEAYRRRLFEAVSRAAAPGAMVVLRSFGEPAGDMP
                     TNRAAEDRSILWGIVDVRPAGQLAT"
     gene            complement(2834..3349)
                     /locus_tag="VFV55_04775"
     CDS             complement(2834..3349)
                     /locus_tag="VFV55_04775"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4943643.1"
                     /translation="MRDLFLVVGRLFPANPAIDAAVTRARIDAPPGEAWRRLMFYEEV
                     PQRPPLLLKLFLPSPVRTQGGAKEPGATVECTYSRGGLAKRITVAEAPRLLRFEVLEQ
                     RLGIEHCITTVEGSYEIRAEGDGCEVALTTRYRGVLRPRWLWRAVERFLAHQLHRHIL
                     RGMGAKREGFR"
     gene            complement(3426..4697)
                     /locus_tag="VFV55_04780"
     CDS             complement(3426..4697)
                     /locus_tag="VFV55_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX4943644.1"
                     /translation="MIEMPGVVADVLRSVGLSTAMKAAGVVTGLLPIPQPILLVGPGA
                     SRRLGQALCGFGHRKILIVTDRVVAELGLMNAFTQALKKGGADFVVFDAITPDAPIPV
                     IEKGIAFYTRRACDAIVAFGGGSAMDAAKVIGYAAANRKHPRKLVGYFKGLRSPPPLY
                     AVPTTAGTGSEVTVAAVISDPEAGTKLVIADTRLVPSMAALDPALMTGLPRDVTAATG
                     MDALTHAVEAFIGQWATDYTDRMALAAVGLVFENLPVACRNGKNLEARERMALAATYA
                     GLAFTRANVGYVHAIAHQLGGKYHTPHGLANAIMLPRVLRYLAPAVTRRLAMLAVAAK
                     VGQEGERATVLAKKFLASIESMSRALGIPPTLDALREVDIPELAKAACWEADTNYPVP
                     RYMSQETCEGILREVLPKQSRPRAHGGRSRR"
     gene            4783..6939
                     /locus_tag="VFV55_04785"
     CDS             4783..6939
                     /locus_tag="VFV55_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943645.1"
                     /translation="MDQTAAGDFGHRGNKARSDGVGMKTVAGISLGAGAQDFEFRCRF
                     LGKPFHVRRIGTNRSTARAEKLLRHWENHADAIGLGVVKDSYRVGEQQLVDKESARLS
                     AIVTRVPVTTGGRLGDILQEWALRHVQSKLGDYFSNAKVLFFSGVTQYKLALAMSEYT
                     PNLEFADALLQLGVPKMLTSLDALELYASGAHYVVDNVPAEVMSSSVAKAWTHFVLRR
                     AMQDATVIVAPVYELDAFGLEELAGKTIITSTVNDRRIAQLKKKGVNLVVDGAPVLLG
                     HVLGPSLLDAMIIAARGKEPDDLLEDDYLEIITGLKVEPRLIYPNGFRRVNRFAFVIH
                     PLSQEYFKNIKPIELLSHISPPLFLDAVEKAMAHAPPFVYSKVTGIKSPTGVEAEGWL
                     ISVGGTPREIMNHSPDFTYRRLLDAARVARSLGAQIMGLGAFTKVVGDAGLTVARRAP
                     LPITTGNSYSASGALWAAHDAMLRLGLVPLARGGAKAHFKAMVVGATGAIGSVCARLL
                     AMVAKEVHLVSPETAKLLSLKGSILKETPDATLFLSSGADRHLADVDMIVTATSGAGK
                     RILDIMKVKPGCVITDVARPLDLPAEEVARRPDVLVIESGEIHLPGTVSMKNIGLPPN
                     VAYACLAETIVLALEGRFENFTVGRNIEWEKVREIYRLGLKHGMKLAAISGVNGPFSN
                     ADIRRVRRLALAARAATKLEPARVSRKRRMSRNSAA"
     gene            complement(7048..8457)
                     /gene="pssA"
                     /locus_tag="VFV55_04790"
     CDS             complement(7048..8457)
                     /gene="pssA"
                     /locus_tag="VFV55_04790"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:NF006946.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX4943646.1"
                     /translation="MDKKSTPPPKTSVLASTWRRLKGRADRRPALADLPCIPVSADHV
                     ATLTGPVAFRETLLALIAQARERILVATLYLQDDDAGREVLSALYAAKAARPELEIAV
                     FVDWHRAQRGLVGESRSPGNAALYRAMALRLGPGVPVYGVPVQTREMMGVMHLKGFIL
                     DDTVLYSGASLNEVYLHRNERYRLDRYHVFQDRHLADSLAGLLLHVILPSPAVHALDS
                     DVAPRTASLRAPIVKFRRVLAKTRYHYAEGTIRSGEVGITPLLGLGGRDNELNAVLLK
                     LVRQARDHLILFTPYFNLPGPLRRGIDAKIKEGCKVTIVLGDKVANDFYIPPEEPFKT
                     IGALPYLYEGNLRRFCKTHQVAIDRGLLNVHSWRHEHHTFHLKGLLVDDDYALLTGHN
                     MNPRAWRMDLENGLLVHDPHKLLRDRNQAELERIVAHTRRLDHYGSLEAIEAYPAPVR
                     RLLKRLAHARMDRLVNHVL"
BASE COUNT         1334 a         2948 c         2950 g         1398 t
ORIGIN      
        1 cccggcacca ccagcaggaa tttcacctcg ccaccccgca acacgcgcat cgcgctctgc
       61 tgctccacct cgaggaaccg cgccgtgcgc cggttgatcg gcaccgtggc gcccgaaagg
      121 cccacgtagg cgcggcgcac gcgcccgtgc gcgaagagtt ccccgaccac ccacttcgcg
      181 gagtcgatgg cggtggcgaa gcagatcgcc tgcgcgccgg cgatgatcgc ggtattcacg
      241 ccgatcacct gagcgttgct gtcgagcagc gggccgccgg agttgccggg gttgagcgcg
      301 gcatcggtct ggatcacgtt gtggatgagc cgccccgtcg tcgagcgcag cgtgcgcccg
      361 agcgcggaaa cgacgccggt ggtgacggtg tgcgtgtagc cgagcgggtt gccgatggca
      421 atggcgagct ggcctacgcg aaggcgcgcc gagtcgccga aaggcgccca cggcaggttt
      481 tcgcgcgcct cgaaatgcag cagcgcgaga tcggtttccg ggtcctcgcc gaccaggctc
      541 gccggcccgc gccgcccgtc cgcgagcgtc atctcgatgc gcttcgcacc gtgcaccacg
      601 tggctgttgg tgagcagata gccgcccggc gcgaagatga agccggatcc cgtgcccgcc
      661 cttccggcct gcccgtggaa cgcgttgatg aggacgaccg cgtcgcgcac ccggtccacg
      721 gcggagacga cggcctgcga gtaggcatcc agcaggccat cgtcgcgatc ggcgggcatg
      781 gagtcggccg gcacggcgtc ggcttccagg gctatgaggc gggggcgggc acccatcggc
      841 gttcctccgg gataaactcc gcaaatggag tcgagatgac gagcagtcaa ggggcgccgc
      901 ccggcgcccc tttcaacgat cccgccggaa cgtggaaccg ccgcttcgag gaagacggct
      961 tccatttcgg gactgcgccc aatacgtggc tcgtcgagca cgccgacctg tttcgccccg
     1021 gccagcgcgt cctgtgcgtg gccgacggag agggccgcaa cagcgtgtgg ctcgccaagc
     1081 gcggcctgga agtcgatgca ttcgacatcg ccggggtggg cgtggagaag gcacgccggt
     1141 tcgcggccga gcacggtgtc acggtcaact tcgccgtcag cgactgcgac gcctacgcct
     1201 ggcagcagga gcattacgac ggcgtcgtgg cgatcttcgt gcagttcgcc gatcccgcga
     1261 tgcgcgggcg cctgttcgcg aacatgaagc gaagcctccg gcccggcggc gtgctcgttc
     1321 tccagggcta cacgccgaag cagctcgagt accgcacggg cggaccgccg atcgcctcgc
     1381 acctctatac ggtggaaatg cttcgcgagg ccttcgcgga cctgaagatc gtcgagctgc
     1441 gaaagtacga ggcgcagctc gcggagggca aggggcactg cggtcgctcg gccctcatcg
     1501 gcctcatcgc ccggcgtccg ggataagccc cgcccccgag cataccggcc gaatggattc
     1561 gctcctcacc gtgatgcccc cgtcgtggct cgtgcgcgcc gccgtcgccg gcgtctggat
     1621 ctacgagggg ctgtggtgca agatcctcgg ccgctcggat aacgagctgc gcgtcgtgaa
     1681 ggccgtgccg cgcttcggag agagattcgg ccctccgttc ctgatggcac tgggctgggt
     1741 ggagctgggg ctgggcgtgt gggtcctctc cggctggacg ccgggactgt gcgcggccgc
     1801 gcaaacactt cttctcgtgg cgctcaacgc caatggcctc ctctggtcgc gccagcacat
     1861 ccacgacccc gccggcatgg tgttcaagaa cttcgccttc ctcatccttg cctgggtcaa
     1921 cgcgggtctg gccgcatgac aggcgatgcg ccgggcaccg cgtgggaggc ggggcgcctc
     1981 gatgccggcc acggcccgcc gcgcgtgctc ttcggccgca tgtacgagga tccggcgatc
     2041 gagctctcgg ccttccggcc cggcggccgc gtgttctgca tcgcctcggc gggctgcacg
     2101 gccctggcgc tggcgccgag ccatccggtc gtcgcggtgg atatcaatcc cgcgcagctc
     2161 acctacgcgg cgcggcgcat tgccggcgag ccgatggtgc gcggcaccgc cgaatccttg
     2221 atggcgttcg ggcggacctt cctgccgctt gcgggctggc ggcgttccac gctcgcaacc
     2281 tttctcgacc tggacgaccc gggcaggcag cgcgaattct ggaaggcgca cctcgacacg
     2341 ctgcgatttc gcgccgcgct gaaggtgctg ctctcgttca cggcgcttcg cgcaggctac
     2401 gcccccggac ttctctccgc gctgccgccg cgcttcgacg aagtgatgcg ccgccgcctc
     2461 gagcgctgct tctccacgca ccccaaccgg tccaacctct gggctcgcgc gcttctcctg
     2521 ggcgaactcg cgcgcgagac accgtcaacg caggcgatgg cgatcgagct cgtgcgcgca
     2581 gacgccgcca cctttctcga aagcgcacct gcgcggagct tcgacggctt cacgctttcc
     2641 aacattctcg acggggcgac ggaggcgtac cgaaggcggc tcttcgaggc ggtttcccgt
     2701 gccgccgcac ccggtgcgat ggtggtgctg cgcagtttcg gcgagccggc gggcgacatg
     2761 cccacgaatc gcgccgccga ggaccgctcg atcctctggg gaatcgtcga cgtgcggccg
     2821 gccgggcaac tcgctacctg aagccctcgc gcttcgcgcc catgccgcgc aggatgtggc
     2881 ggtggagctg gtgggcgagg aaccgttcca ccgcgcgcca cagccagcgt ggccgcagga
     2941 cgccgcggta cctcgtggtg agcgccactt cgcacccgtc gccctcggcg cggatctcgt
     3001 aggaaccttc cacggtggtg atgcagtgct cgatgcccag gcgctgttcg agcacctcga
     3061 agcgcaggag gcgcggcgct tccgcgacgg tgatgcgctt ggcgaggccg ccgcggctgt
     3121 aggtgcactc gacggttgcg cccggttcct tcgcgccgcc ttgcgtgcgc acggggctcg
     3181 gcaggaacag cttcaggagc agtggcgggc gctgcggcac ctcttcgtag aacatgagcc
     3241 ttcgccacgc ttccccgggg ggcgcgtcga tccgggcgcg ggtgacagcg gcatcgatgg
     3301 cggggttggc ggggaagagc cgcccgacga cgaggaagag gtcgcgcaca ttcactcctg
     3361 gttccgggcc gtcgctgcga gatgttcgct tccaggccgt gcgcattcta accgcaggcc
     3421 gcggtttacc ggcgactgcg tccgccatgc gcgcgtggcc gggattgctt cggcagaacc
     3481 tcccggagaa tcccctcgca ggtctcctgc gacatgtacc gcggcaccgg gtagttggta
     3541 tccgcctccc agcaagccgc cttggccagt tcgggaatat ccacctcgcg cagcgcgtcg
     3601 agcgtgggcg gaatcccgag ggcccggctc atcgactcga tcgacgcgag gaatttcttc
     3661 gcgagcaccg tcgcccgctc gccttcctgg cccaccttgg ccgcgacggc gagcatcgcc
     3721 aatcgcctgg tcaccgcggg agccaggtag cgcaacacgc gcggcagcat gatcgcgttt
     3781 gccagaccat ggggcgtgtg gtacttgccg ccaagctggt gcgcgatggc atgcacgtag
     3841 cccacgttcg cccgcgtgaa cgccagcccc gcgtaggtcg ccgcgagagc catgcgctcg
     3901 cgcgcctcca ggttcttccc gttccgacag gcgaccggca ggttctcgaa gacgagcccc
     3961 acggccgcga gcgccatccg gtccgtgtaa tccgtggccc actgcccgat gaacgcctcc
     4021 acggcgtgag tcagcgcgtc catgcccgtc gccgccgtca cgtcacgcgg cagccccgtc
     4081 atcaacgcgg gatcgagggc cgccatcgag ggcacgaggc gcgtgtccgc gatcaccagc
     4141 ttcgttccgg cctccgggtc cgagatcacc gcggccacgg tcacttcgga cccggtccct
     4201 gccgtggtcg gcacggcgta taggggcggt ggcgagcgca ggcccttgaa gtacccgacg
     4261 agcttgcgcg ggtgcttcct gttggcggcc gcatagccga tcactttcgc cgcatccatg
     4321 gccgagcctc cgccgaatgc cacgatcgcg tcgcaggccc gccgcgtgta gaacgcgatg
     4381 cccttctcga tcaccgggat gggcgcatcc ggcgtgatcg catcgaacac gacgaagtcg
     4441 gcgccgccct tcttcaatgc ctgggtgaac gcgttcatca ggcccagttc cgccacgacg
     4501 cggtccgtca cgatcaggat cttgcggtgg ccgaagccgc acagcgcctg ccccaggcgc
     4561 cggctcgccc ccgggcccac cagcaggatc ggctgcggaa tgggcagcag gcccgtcacc
     4621 acacccgccg ccttcattgc cgtggaaagc cccacggagc gcagcacgtc cgccaccaca
     4681 cccggcatct cgatcatctt tgcgcccgaa tcttcgacct ggacccggcc agcttgcacc
     4741 aaacccgggg gccgggccac ggttgcgcgc gcgtttgtcg acatggatca aacggcggcg
     4801 ggcgatttcg ggcaccgtgg caacaaggcg aggtccgatg gggtcggcat gaagacggtg
     4861 gcgggtatca gcctcggggc gggtgcgcag gatttcgagt tcaggtgccg ctttctcggc
     4921 aagccgttcc acgtccggcg catcggcacg aaccgcagca cggcccgggc ggaaaagctc
     4981 ctccggcact gggagaacca cgccgatgcc atcggcctgg gcgtggtgaa ggacagctac
     5041 cgcgtcggcg agcaacagct tgtagacaag gaaagcgcgc ggctttccgc catcgtgacc
     5101 cgcgtgcccg tcaccaccgg cgggcgcctg ggcgacatcc tgcaggaatg ggcgctgcgc
     5161 cacgtccagt cgaagctggg cgactacttc agcaacgcga aggtgctgtt cttctcgggc
     5221 gtgacccagt acaagctggc gctcgcgatg tcggagtaca cgcccaacct cgagttcgcc
     5281 gatgcgctgc tgcagctggg cgtgcccaag atgctcacct cgctcgatgc cctggagctc
     5341 tacgcgagcg gcgcgcatta cgtggtggac aacgtgcccg cggaggtcat gtcctcgtcg
     5401 gtggccaagg catggaccca cttcgtgctg cgccgggcca tgcaggatgc caccgtcatc
     5461 gtggcgccgg tgtacgagct ggacgccttc ggcctcgagg agctggccgg gaagacgatc
     5521 atcacctcca ccgtcaacga ccggcgcatc gcgcagttga agaagaaggg ggtgaacctg
     5581 gtggtcgacg gcgcgcccgt gctcctcggc cacgtgctgg gcccgagcct cctcgacgcg
     5641 atgatcatcg ccgccagggg caaggaaccc gacgacctcc tcgaggacga ctacctggag
     5701 atcatcaccg gcctcaaggt cgagccgcgc ctcatctatc ccaacggctt ccggcgcgtg
     5761 aaccgcttcg cgttcgtgat ccacccgctg tcgcaggagt acttcaagaa catcaagccc
     5821 atcgagctgc tctcgcacat ctcgccgccg ttgttcctgg acgcggtgga gaaggcgatg
     5881 gcccatgcgc cgcccttcgt gtactcgaag gtcaccggca tcaagtcgcc gaccggcgtc
     5941 gaggccgagg gctggctcat ctcggtcggc ggcacgccgc gcgaaatcat gaaccacagc
     6001 cccgacttca cctaccggcg cctcctggac gcggcccgcg tggcgaggag cctcggcgcg
     6061 cagatcatgg gcctgggcgc cttcaccaag gtggtgggcg acgcgggcct cacggtggcc
     6121 cggcgcgcgc cgctgcccat caccaccggt aacagctaca gcgcctcggg tgctctctgg
     6181 gcggcgcacg acgccatgct gcgcctcggc ctggttccgc ttgcccgggg cggggcgaag
     6241 gcgcatttca aggcgatggt ggtgggcgcc accggcgcga tcggctcggt ttgtgcgcgc
     6301 ctgctggcga tggtcgccaa ggaggtccac ctcgtctcgc cggaaacggc caagctcctc
     6361 tcgctcaagg gctcgatcct gaaggagacg cccgacgcca cgctcttcct ctcgtccggc
     6421 gccgacaggc acctcgcgga cgtggacatg atcgtcacgg cgacctccgg ggcgggcaag
     6481 cggatcctcg acatcatgaa ggtgaagccc ggttgcgtga tcacggacgt ggcccgtccc
     6541 ctcgaccttc cggcggaaga ggtcgcacgg cgcccggacg tgctggtcat cgaatccggc
     6601 gagatccacc ttcccggaac ggtctcgatg aagaacatcg gcctgccgcc gaacgtggcc
     6661 tatgcctgcc tcgccgagac gatcgtcctt gcactcgaag ggcggttcga gaacttcacc
     6721 gtcgggcgca acatcgagtg ggagaaggtg cgcgagatct accgcctcgg cctcaagcac
     6781 ggcatgaagc tcgcggccat ctccggcgtg aacgggccgt tctcgaatgc cgacatcagg
     6841 cgggtgcgcc gtctcgcgtt ggccgcgcgg gcggcgacga agctcgagcc tgcccgcgtt
     6901 tcgcgcaagc gtcgcatgtc gcggaatagc gccgcgtaag cctggcccaa tcgaacgcgc
     6961 gggcctgacc acggcaactt ccacggcgcg cgggtcgtgg ccaacgggtc ggtcgtcctg
     7021 caacccgtcg gcgccttgcg cgttccttta cagcacgtgg ttcaccaatc gatccatgcg
     7081 cgcgtgcgcc aggcgcttga gcaggcgccg gacgggggca ggataggcct cgatcgcttc
     7141 gagtgacccg tagtgatcca ggcgccgggt gtgcgccacg atccgttcca gctcggcctg
     7201 gttccggtcg cgcaacagct tgtgcgggtc gtggacgagc aggccgttct cgaggtccat
     7261 gcgccaggcg cggggattca tgttgtggcc cgtgaggagg gcgtagtcgt cgtccaccag
     7321 caggcccttg agatgaaatg tgtgatgctc gtggcgccac gaatgcacgt tgagcagccc
     7381 gcgatcgatg gcgacctggt gggtcttgca gaaacggcgc aggttgccct cgtagaggta
     7441 gggaagcgca ccgatcgtct tgaagggttc ctcgggcggg atgtagaaat cgttcgcgac
     7501 cttgtcgccc aggacgatgg ttaccttgca tccttccttg atcttcgcgt cgatgcccct
     7561 gcgcaggggg ccgggaaggt tgaaataggg cgtgaacagg atgagatggt cccttgcctg
     7621 gcgaaccagt ttcagcagga cggcgttgag ctcgttgtcc ctgcccccga ggccgagcag
     7681 cggcgtgatg cctacttcgc cgctgcgaat ggtgccctcg gcatagtggt agcgggtctt
     7741 cgccaggacg cggcggaact tgacgatggg ggcgcgcagg gaggccgttc gtggcgcgac
     7801 gtcgctgtcc agtgcgtgta ccgccgggct ggggaggatg acgtgcagca gcaggccggc
     7861 aaggctgtcg gccaggtgtc gatcctggaa cacgtggtat cgatccagcc ggtagcgctc
     7921 gttccgatgc aggtagacct cgttgaggct ggcgccgctg tagaggaccg tgtcgtcgag
     7981 gatgaagccc ttcaggtgca tgactcccat catctcccgg gtctggaccg gaacgccgta
     8041 aaccggaacg ccgggaccca ggcgcagcgc catcgcccgg taaagggccg cgttgcccgg
     8101 gctcctggat tcgcccacga gcccccgctg ggcgcgatgc caatcgacga agaccgcgat
     8161 ctccagctcc ggacgcgccg ccttggcggc atacagggcc gaaagcacct ccctgcccgc
     8221 atcgtcgtcc tggaggtaga gcgtcgcgac caggatccgc tcccgggcct gggcgatgag
     8281 ggccagcagg gtttcgcgaa acgccaccgg acccgtcaag gtcgcgacgt gatccgccga
     8341 caccggaatg cacggcaggt ccgccagtgc gggcctgcgg tccgcgcggc ccttcaggcg
     8401 ccgccaggtg ctggccagga cggaggtctt cgggggagga gttgatttct tgtccacggg
     8461 tttccgaagt atcgacgagg ccgcggggga agtcgatcgc gatcctgggc acgtgggtcg
     8521 aggaggtggt ggcggtggaa gtcgatcgca atcctaggca cgcaagcttg accgtcggat
     8581 gggtcccaaa aatccgaggc caggcaaaac aagccaggcc tcggattttt
//
LOCUS       DASUEV010000147         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_960476, whole genome shotgun sequence.
ACCESSION   DASUEV010000147 DASUEV010000000
VERSION     DASUEV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960476"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFV55_04795"
     CDS             <1..753
                     /locus_tag="VFV55_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4943647.1"
                     /translation="NQKGGVGKTSIAVNLAASLAHYGKRVLLVDLDPQGNATTGSGVD
                     KTALDRTVYGILLGECSVAEARARAEGGYDVVGSNRELAGAEVELIGIERREYRLREA
                     LAPVLADYDYVLIDCPPALNMLTVNGFTAADAVIIPMQCEYYALEGLSDLVGTLRKVK
                     QNLNPGIEIEALVRTMYDPRSTLTVQVSDELKRHYGDKVFDTVIPRNVRIAEAPSFGK
                     PVLLHDPASKGAHAHLAFARELLERSGAAVPA"
     gene            771..1628
                     /locus_tag="VFV55_04800"
     CDS             771..1628
                     /locus_tag="VFV55_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX4943648.1"
                     /translation="MIKHKGLGRGLDALLGGGSKSRSDGELFQLAVRSLRPGKYQPRT
                     RMDQTSLEELAESIRVRGILQPVVVRPVDDGDYEILAGERRWRAAKLAGLERIPALVR
                     EVSDDAALGIGLIENIQREDLNPIEESAGLKRLIDEFRLTHEEVAKAVGRSRSAVTNL
                     LRLLELAKAVQEMLLDGRLDMGHARALLGVSKARQVELAEQVAAKGLSVRETERLVQH
                     SADAPKAGAKAAKPRLDADTRRLQDELSEALGASVTVHPRKAGRGSLVIDYASLEQLD
                     GIVSRLKRP"
     gene            1941..2111
                     /locus_tag="VFV55_04805"
     CDS             1941..2111
                     /locus_tag="VFV55_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943649.1"
                     /translation="MLVMAEAAKWAVSLVGLASLLSGTAGVEAARAAPGAVVIAFCVA
                     WAAPLLALVKRD"
     gene            2116..2937
                     /gene="atpB"
                     /locus_tag="VFV55_04810"
     CDS             2116..2937
                     /gene="atpB"
                     /locus_tag="VFV55_04810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX4943650.1"
                     /translation="MAAQGSLTPSDYIAHHLTNLKVTVGEGGFWVVNIDTILMSWLLG
                     LVGLGLIWMVARSATSEVPGRLQSFIEMFFEFVDGTVRDVFPGSRAFLGPLALTIFAW
                     VFMMNLADLVPIDWVATVSHGLGAPAWKAVPTTDLNTTFAMSFSVLFLIIFFSFKAKG
                     AGGFVHELFTAPFGSNPLLWIPNLALNIIELLAKPVSLGMRLFGNMYAGELIFMLLGL
                     LGTIAGFSAGGLLAGGAAGVLTWAWAVFHILIILLQAFIFMVLSVVYIAMAHEHH"
     gene            2984..3250
                     /gene="atpE"
                     /locus_tag="VFV55_04815"
     CDS             2984..3250
                     /gene="atpE"
                     /locus_tag="VFV55_04815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX4943651.1"
                     /translation="MAQFLAQIQAFTALGIGIIIGLGAIGACIGIGIMGSKFLEAAGR
                     QPELIPQLQKFMFLLAGLIDAAFLIGVGIAMFFGFANPLLSVIK"
     gene            3279..3749
                     /locus_tag="VFV55_04820"
     CDS             3279..3749
                     /locus_tag="VFV55_04820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX4943652.1"
                     /translation="MNINATLFIQALSFAALIWFAVRFIWPPLNRAIDERNRRIADGL
                     AAAEKGKAALVEAERKGDAEIRQARERASGVLGESEKRGAQIVDEARQQAKAEADRII
                     AAAREQIASEVAKAKTDLREQVAVLAVAGAEKILQREVDANAHAEMLGKLKAQL"
     gene            3757..4293
                     /locus_tag="VFV55_04825"
     CDS             3757..4293
                     /locus_tag="VFV55_04825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX4943653.1"
                     /translation="MAEIATLARPYAEASFRSALEAKDLAGWSDGLALAGAIASDAQM
                     ADLLGNPRLTRMQKLELFYGVGGDGLSAHVRNLVTILVDGARASLLPEIAAQFDALKR
                     AHESVLKVRIVSARALADAEYQDLVATLGRKYGRRIEATVEIDAALIGGARVHVGDEV
                     IHASMRDALAQMAVALSR"
     gene            4322..>5530
                     /gene="atpA"
                     /locus_tag="VFV55_04830"
     CDS             4322..>5530
                     /gene="atpA"
                     /locus_tag="VFV55_04830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX4943654.1"
                     /translation="MQINPAEISELIKSKIQNLQVVAEKRTEGSVISVTDGICRIHGL
                     ADVMQGEMLEFPGNTFGMALNLERDSVGAVVLGAYERISEGDTVKCTGRILEVPVGAE
                     LIGRVVNSLGQPIDGKGPVGAKATEPIEKVAPGVIWRKSVSQPVQTGLKSIDAMVPVG
                     RGQRELIIGDRQTGKTAVAIDTIINQKGKDLICIYVAIGQKASTIVNVVRKLEEHGAM
                     AYTIVVAASASDPAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEEYVEKFTNGAVKGKTGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGSAQTKVIKKLGGG
                     VRLALAQYREL"
BASE COUNT          922 a         1932 c         1823 g          853 t
ORIGIN      
        1 aaccagaagg gcggcgtcgg caagacctcg attgccgtga accttgccgc gagcctcgcg
       61 cactacggca agcgggtgct gctcgtcgac ctcgatccgc agggcaacgc caccaccggg
      121 agcggcgtcg acaagaccgc gctcgaccgc accgtctacg gcatcctgct cggcgaatgc
      181 agcgtggccg aggcgcgggc gcgcgcggag ggcggctacg acgtcgtcgg ctccaaccgc
      241 gagctagccg gcgccgaggt cgagctgatc gggatcgaac gccgcgaata ccgtttgcgt
      301 gaagcgctcg ctccggtgct cgccgactac gactacgtgc tcatagattg cccgccggcc
      361 ctgaacatgc tgacggtgaa cggctttacc gcggccgacg ccgtgatcat cccgatgcag
      421 tgcgagtact acgcgctcga ggggctctcc gacctcgtgg gaacgctgcg caaggtgaag
      481 cagaacctga accccgggat cgagatcgag gcgctggtgc gcacgatgta cgacccgagg
      541 agcaccctca ccgtgcaggt ctccgacgaa ctgaagcgcc actacggcga caaggtcttc
      601 gacaccgtga tcccgcgcaa cgtgcgcatc gccgaggcgc cctccttcgg caagcccgtg
      661 ctcctgcacg accccgcgtc gaagggggcc cacgcgcacc tcgccttcgc gcgcgagctc
      721 ctggaacgaa gcggcgccgc cgtaccggcc tgagtacaag gaacgattcg atgatcaagc
      781 acaagggact gggccgcgga ctcgacgcgc tgctgggcgg cgggtcgaag tcgaggtccg
      841 acggcgagct gttccagctc gcggtgcgct cgctgcgccc cggcaagtac cagccgcgca
      901 cgcgcatgga ccagacctcg ctcgaggaac tcgccgaatc gatccgcgtg cgcggcatcc
      961 tgcagcccgt ggtggtgcgc cccgtggacg acggcgacta cgagatcctc gcgggcgaac
     1021 gccgctggcg cgccgcgaag ctcgcaggcc tggagcgcat tcccgccctg gtgcgggaag
     1081 tgtccgacga cgcggcgctc ggcatcggcc tcatcgagaa catccagcgc gaggacctca
     1141 accccatcga ggaatcggcg gggctcaagc gcctcatcga cgagttccgg ctcacgcacg
     1201 aggaagtggc caaggccgtg ggccggtcgc gcagcgccgt caccaacctg ctgcgcctgc
     1261 tggagctcgc caaggcggtg caggaaatgc tgctcgacgg gcgcctcgac atgggccacg
     1321 cgcgggcgtt gctcggcgta agcaaggcaa ggcaggtgga gctggccgag caggtggccg
     1381 cgaaggggct ttccgtgcgc gagaccgaac ggctggtcca gcattccgcg gacgcaccga
     1441 aggccggcgc gaaggcggcc aagccgcgcc tcgacgccga tacgcggcgc ctgcaggacg
     1501 agctttcgga ggctctcggc gcatccgtca ccgtgcatcc gcgcaaggcg ggacggggca
     1561 gcctggtcat cgattacgcg agcctcgagc agctcgacgg gatcgtttcg cgcctcaagc
     1621 gaccctgacc ggaaccatat tgcgttgccg caacttttga ccccatgacg ccttccgggc
     1681 tagaatcttc aggtttgtcg tcaccgcgcc caagcccaaa gcccgcgccg acctccgcga
     1741 ttttcgacgg ccggcggcag gttctggtgc aggccgggct ggtcgcttgc tgcagtgcag
     1801 caggcttcgt gctcggcggg cgggaaagcg cgcttgctgc gctctccgga ggctcggccg
     1861 ccctggcagg caccctggca ttcgtcgggg tccttctgtg gcgcaaccgc ccggcgccga
     1921 cgccctggca ggcccttcgg gtgctggtca tggcggaggc ggccaagtgg gccgtgtcgc
     1981 tggtgggcct cgcctcgctc ctttccggca cggccggggt ggaggcggca cgcgccgcgc
     2041 ctggcgctgt agtgatcgca ttctgtgtcg cgtgggcagc cccgctgctc gcactagtga
     2101 aaagagactg agcgtatggc cgcccaaggt tccctcacgc catcggatta catcgcccac
     2161 cacctcacca acctcaaggt gacggtcggg gagggcggct tctgggtcgt caacatcgac
     2221 acgatcctca tgtcctggct cctgggtctc gtgggcctgg ggctcatctg gatggtcgcc
     2281 aggagcgcca cgtccgaggt cccggggcgg ctgcagtcct tcatcgagat gttcttcgag
     2341 ttcgtggacg gcaccgtgcg cgacgtattc cccggctcgc gcgccttcct gggcccgctg
     2401 gcgctgacca ttttcgcgtg ggtgttcatg atgaacctcg cggacctggt ccccatcgac
     2461 tgggtggcca ccgtctcgca cggcctgggc gcgccggcat ggaaggcggt cccgaccacg
     2521 gacctcaaca ccaccttcgc catgtcgttc tcggtgctct tcctcatcat cttcttcagc
     2581 ttcaaggcca agggcgccgg cggcttcgtg cacgagctgt tcaccgcacc gttcggctcc
     2641 aacccgctgc tgtggatccc gaacctcgcg ctgaacatca tcgagctgct ggccaagccc
     2701 gtatcgctcg gcatgcggct tttcggaaac atgtatgcgg gcgagctcat cttcatgctg
     2761 ctcgggctgc tgggcaccat cgcgggcttc agcgcgggcg ggctgctggc cggcggcgcc
     2821 gcgggcgtcc tcacctgggc ctgggccgtg ttccacatcc tgatcatcct gctgcaggca
     2881 ttcatcttca tggtgctgtc ggtcgtctac atcgcgatgg cccacgagca ccactgaaaa
     2941 caaaacctcc accccaacac ccgcacctgc gaaaggaaag tccatggctc agttcctcgc
     3001 ccaaatccaa gccttcacgg cgctcggcat cggcatcatc atcggcctgg gcgccatcgg
     3061 cgcctgcatc ggcatcggca tcatgggctc gaagttcctg gaggccgccg gccgccagcc
     3121 cgagctgatc ccgcaactgc agaagttcat gttcctgctc gccggcctca tcgacgcggc
     3181 gttcctgatc ggcgtcggca tcgcgatgtt cttcggcttc gcgaacccgc tgctgtcggt
     3241 catcaagtaa tccacgccca acggaaaggc cgtacgccat gaacatcaat gcgaccttgt
     3301 tcatccaggc gctttcgttc gcggccctca tctggttcgc ggtcaggttc atctggccgc
     3361 cgctcaaccg cgccatcgac gagcgcaaca ggcgcatcgc cgacggtctc gcggccgccg
     3421 agaagggcaa ggccgcgctg gtggaagccg agcgcaaggg agacgccgag atcaggcagg
     3481 cgcgtgagcg agcctcgggc gtgctgggcg aaagcgaaaa gcgcggcgcg cagatcgtcg
     3541 acgaggcgcg ccagcaggcg aaggccgagg ccgaccggat cattgccgcc gcacgcgagc
     3601 agatcgcctc ggaagtcgcc aaggcgaaga cggacctgcg cgagcaggtc gccgtcctgg
     3661 cggtggccgg cgcggagaag atcctgcagc gcgaggtcga tgcgaacgcc cacgccgaga
     3721 tgctcggcaa gctgaaggcc cagctctagg cgcgccatgg ccgagatcgc caccctcgcc
     3781 cgcccctacg ccgaagcgtc gttccgctcg gcgctggaag ccaaggacct cgccggatgg
     3841 tccgacgggc tcgcgctcgc cggggccatc gcatccgacg cgcagatggc cgacctgctc
     3901 ggcaacccgc gcctcacccg catgcagaag ctcgagcttt tctacggcgt gggcggcgac
     3961 gggctttccg cgcacgtgcg caacctcgtg acgatcctgg tcgacggcgc ccgggcgtcg
     4021 ctgctgccgg aaatcgccgc ccagttcgac gcgctcaagc gcgcccacga aagcgtcctc
     4081 aaggtgcgca tagtgagcgc aagggcgctg gcggacgccg agtaccagga cctcgtcgcc
     4141 accctcggcc gcaagtacgg ccgcaggatc gaggccacgg tcgaaatcga cgccgcgctc
     4201 atcggcggag cccgcgtcca cgtcggcgac gaggtcatcc acgcctccat gcgcgacgcg
     4261 ctcgcccaga tggccgtcgc gctctcgcgc tgacgctgcc cacacgcttt cggagcccca
     4321 catgcaaatc aatcctgcgg aaatcagcga actcatcaag tcgaagatcc agaacctcca
     4381 ggtggtcgcg gaaaagcgca ccgaggggtc ggtgatctcg gtgaccgacg gcatctgccg
     4441 catccacggc ctcgcggatg tcatgcaggg cgagatgctc gaattcccgg gcaacacctt
     4501 cggcatggcg ctgaacctcg agcgcgattc cgtcggcgcc gtcgttctcg gcgcctacga
     4561 gcgcatctcg gaaggcgaca ccgtcaagtg caccggccgc atcctcgagg tgccggtcgg
     4621 cgccgagctg atcggccgcg tcgtcaactc gctcggccag cccatcgacg gcaagggtcc
     4681 ggtcggcgcc aaggcgaccg agccgatcga gaaggtcgcg ccgggcgtca tctggcgcaa
     4741 gagcgtttcg cagccggtgc agaccggact gaaatccatc gacgcgatgg tgccggtggg
     4801 ccgcggccag cgcgagctca tcatcggcga ccgccagacg ggcaagaccg ccgtggccat
     4861 cgacacgatc atcaaccaga agggcaagga cctcatctgc atctacgtgg cgatcgggca
     4921 gaaggcctcg acgatcgtga acgtggtgag gaagctcgag gagcacggcg cgatggccta
     4981 caccatcgtc gtcgcggcct ccgcctccga ccccgccgcg atgcagtaca tcgcccccta
     5041 ctccggctgc acgatgggcg agtacttccg cgaccgcggg caggacgcgc tcatcatcta
     5101 cgacgacctc accaagcagg cgtgggcgta ccgccagatc tcgctgctgc tgcgccgccc
     5161 gccgggccgc gaggcgtatc ccggcgacgt gttctacctg cactcgcgcc tgctcgagcg
     5221 cgccgcgcgc gtgaacgagg agtacgtgga gaagttcacc aacggcgcgg taaagggcaa
     5281 gaccggctcg ctcacggcgc ttcccgtcat cgagacgcag gcgggcgacg tgtcggcgtt
     5341 cgtgccgacc aacgtgatct cgatcacgga cggccagatc ttcctcgaaa ccgacctctt
     5401 caacgcgggc atccggccgg ccatcaacgc gggcatctcg gtgtcgcgcg tgggcggctc
     5461 cgcgcagacc aaggtcatca agaagctggg cggcggcgtg cgcctggctc tcgcgcagta
     5521 ccgcgagctc
//
LOCUS       DASUEV010000148         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1532644, whole genome shotgun sequence.
ACCESSION   DASUEV010000148 DASUEV010000000
VERSION     DASUEV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532644"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFV55_04835"
     CDS             <1..842
                     /locus_tag="VFV55_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX4943655.1"
                     /translation="PGKPAYYSDDGPPDKVPADIASIPDAVPRDEPYHRYANRPYTVF
                     GRTYAPAVNDDPMKERGLASWYGRKFHGQRTSSGEIYDMFAMTAAHKTLPIPSYARVT
                     SLKSGQSVVVRVNDRGPFHDGRVIDLSYAAAARLGIAGPGSGPVEVERVFARDGGVRV
                     AAATPAATTPPSATLAFPLASTEPPQPRAAEVATPLVSAESTGLYLQLGAFSSVDNAE
                     SFRDRIARELPWLLEPIRLAAAGGLHRVRLGPYKTREEAQAIADKIRASLDFAPLILP
                     ATR"
     gene            853..1977
                     /locus_tag="VFV55_04840"
     CDS             853..1977
                     /locus_tag="VFV55_04840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX4943656.1"
                     /translation="MKRLSALFLALLLPLAAAAQAITPPPVAARAFYLLDTLSGQALA
                     AQSEEDRFEPASLTKLMTAYLVFAAIRDHKLDPSMTAVVSERAWKAGGSRMFIEPRKP
                     VTIADLMRGMIVQSGNDATIALAEAVAGTEETFAQLMNREAKRLGMRNSNFVNSTGLP
                     AANHYATARDMAVLAAAVIRDFPELYSLYSQKEFTWNGITQANRNRLLWLDPTVDGVK
                     TGFTEAAGYCLIASAKRGERRLVSVVMGAQSDALRMSENQKLLNFGFLAYDTQRLYRK
                     GEVVASPEIFKGTSATVKLGFDHDVWLTLPRDRFQGMKAQLQTVQPFVAPYSRGQKAG
                     IMKLARDNAPVAEVAVVALEDVPAAGYLARGWDTIRMYFR"
     gene            1983..2855
                     /locus_tag="VFV55_04845"
     CDS             1983..2855
                     /locus_tag="VFV55_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEX4943657.1"
                     /translation="MTTPIAHFNGELLPLDRIRISPLDRGFIFGDGVYEVIPAYGGKL
                     LRAREHFRRLQRSMDEIRLANPHTTDEWIAEVNRLLAHHPGDQSVYIQVTRGVPPKRD
                     HVIPTGLTPTVFMMANPLSTPSRELVEKGVACVTAKDFRWEKCHIKSTSLLGNVLARQ
                     ISADAGATETILFRDGFLTEAAASNVFIVKDGAVAAAPQDNHILLGITYELLTELARE
                     GKLRYEIRPIAEAEVRSADEIWLTSSTKEVLAVTTLDGKPVGAGVPGPVFRRMHALFQ
                     AYKATLAATPVAAD"
     gene            2874..3152
                     /locus_tag="VFV55_04850"
     CDS             2874..3152
                     /locus_tag="VFV55_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX4943658.1"
                     /translation="MQADGPESKPLLTFPTDFPIKVMGRREEGYAQAILEVVLRHAPD
                     FDPATMEMRPSKAGTYLSLTVTINARSREQLDALYRDLSGHPLVMMVL"
     gene            3190..3801
                     /gene="lipB"
                     /locus_tag="VFV55_04855"
     CDS             3190..3801
                     /gene="lipB"
                     /locus_tag="VFV55_04855"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX4943659.1"
                     /translation="MTPAIRDLGRAEYEPVWRAMQAFTTARDDSTPDELWFVEHPPVF
                     TLGLAAKPEHVLDAGAIPVVRIDRGGQVTWHGPGQLVAYVLLDLKRAGYGVKELVRRL
                     EQSVIDLLAGYGVTGARQAGMPGVYVNGAKIAAVGLRVSRGCSYHGISLNVDADLAAF
                     SRINPCGYPGLASTRMADHGVRDSMEFVHARLADALEKCLTPH"
BASE COUNT          617 a         1426 c         1203 g          577 t
ORIGIN      
        1 cgcccggcaa gcctgcctac tattccgacg acggtccgcc ggacaaggtg ccggccgaca
       61 tcgcctccat tcccgatgcc gtccctcgcg acgagcccta tcaccgctac gcgaaccgcc
      121 cctacacggt attcgggaga acctacgcgc cggcggtgaa cgacgacccg atgaaggagc
      181 gcggcctcgc ctcgtggtac gggcgcaagt tccacggcca gcgcacgtcc tccggcgaga
      241 tctacgacat gttcgcgatg accgccgcgc acaagacgct gcccataccg agctacgcgc
      301 gggtgacgag cctcaagtcc ggccagtcgg tggtggtgcg ggtgaacgac cgcggaccct
      361 tccacgacgg gcgcgtgatc gacctctcgt atgcggccgc ggcgaggctc ggcatcgccg
      421 gcccgggcag cgggccggtc gaggttgaac gcgttttcgc gcgcgatggc ggggtgcgcg
      481 tcgcggcggc gacaccggcc gccaccaccc cgccatcggc tacccttgcg ttcccgcttg
      541 catccacgga accgccgcag ccgcgcgcgg cggaagtcgc cacgcccctc gtctcggccg
      601 agtccacggg cctctacctg cagctcggcg cgttctcgag tgtcgacaac gcggagagtt
      661 tccgcgaccg catcgcgcgc gagctgccct ggctgctgga gccgatccgg ctcgccgcgg
      721 cgggcggcct gcaccgcgtg cgcctggggc cctacaagac ccgcgaggag gcacaggcca
      781 ttgccgacaa gatccgcgcc tccctcgact tcgctcccct gatccttccc gcaacccgat
      841 gaatgctcct cgatgaaacg cctgtccgca ctcttcctcg cgctccttct tccgcttgcc
      901 gccgcagccc aggccatcac gccgccgccc gtcgcggcac gcgccttcta cctcctcgac
      961 acgctctcgg gccaggccct ggcggcgcag tccgaggagg atcggttcga gccggcctcg
     1021 ctcaccaagc tcatgaccgc ctacctcgtg ttcgcggcga tccgcgacca caagctcgac
     1081 ccgtcgatga ccgccgtggt ctccgagcgt gcgtggaagg cgggcggctc gcgcatgttc
     1141 atcgaaccga ggaagcccgt caccatcgcc gacctcatgc gcggcatgat cgtgcagtcc
     1201 ggcaacgacg ccaccatcgc gctcgccgag gcggttgccg gcaccgagga gaccttcgcc
     1261 cagctcatga accgcgaggc gaagcgcctg ggcatgcgca actcgaactt cgtcaattcc
     1321 accgggctgc cggccgcgaa ccactacgcc accgcgcgcg acatggcggt gctggccgct
     1381 gcggtgatcc gcgacttccc ggagctgtac agcctgtact cccagaagga attcacctgg
     1441 aacggcatca cgcaggccaa ccgcaaccgc ctgctgtggc tcgacccgac ggtcgacggc
     1501 gtgaagaccg ggttcaccga ggccgccggc tactgcctca tcgcctcggc gaagcgcggc
     1561 gagcggcgcc tggtctccgt ggtgatgggc gcgcagtccg acgccctgcg catgtcggaa
     1621 aaccagaagc tcctcaactt cggcttcctc gcgtacgaca cccagcgcct ctacaggaag
     1681 ggcgaggtcg tcgccagccc cgagatcttc aagggcacca gcgcaacggt gaagctgggc
     1741 ttcgaccacg atgtctggct gacgcttccg cgcgaccgct tccaaggcat gaaggcgcaa
     1801 ttgcagacgg tgcagccctt cgtggcgccc tactcccggg ggcagaaagc ggggataatg
     1861 aagctcgccc gcgacaatgc ccccgtcgcc gaggtcgccg tcgtggccct cgaggacgtg
     1921 ccggcggccg ggtacctcgc ccgcggatgg gacacgatcc gcatgtactt ccggtgaccg
     1981 ccatgaccac cccgatcgcg cacttcaacg gcgagctgct gcccctcgac cggatccgca
     2041 tctcgcccct cgaccgcggc ttcatcttcg gcgacggcgt ctacgaggtg attccggcct
     2101 acggaggaaa gctcctgcgc gcccgcgagc atttccggcg cctgcagcgc tcgatggacg
     2161 agatcaggct cgccaacccg cacaccaccg acgagtggat cgcggaggtg aaccggcttc
     2221 tcgcccacca tcccggcgac cagtcggtct acatccaggt cacgcgcggc gtcccgccga
     2281 agcgcgacca cgtgataccc acgggactca cgcccaccgt gttcatgatg gcgaacccgc
     2341 tgtccacgcc ctcgcgcgag ctggtggaaa agggcgtggc ctgcgtcacg gcgaaggact
     2401 tccgctggga aaagtgccac atcaagtcca cgtcgctcct cggaaacgtg ctcgcgcgcc
     2461 agatttcggc cgatgccggc gccaccgaga cgatcctctt ccgcgacggc tttctcacgg
     2521 aagccgcggc ctccaacgtg ttcatcgtca aggacggcgc cgtggccgcc gcgccgcagg
     2581 acaaccacat cctgctcggc atcacctacg agctgctcac cgaactcgcg cgcgagggca
     2641 agctgcgcta cgaaattcgc cccatcgcgg aagccgaggt gcgctccgcc gacgagatct
     2701 ggctcacctc gtccacgaag gaagtgctcg ccgtcaccac gctcgacgga aagcccgtgg
     2761 gcgccggcgt gcccggaccg gttttccgcc gcatgcacgc ccttttccag gcgtacaagg
     2821 cgacgctcgc ggccacgccg gtggccgccg actgagggag gtccggctcg gccatgcagg
     2881 cggacggccc ggaatcgaag cctctcctca cctttccgac ggactttccc atcaaggtga
     2941 tgggccggcg cgaggaaggg tacgcgcagg ccatcctcga agtggtgttg cgccacgccc
     3001 ccgacttcga tcccgccacg atggaaatgc gcccctcgaa ggcgggcacc tacctgtcgc
     3061 tcaccgtcac catcaatgcg cgctcgcgcg agcagctgga cgccctgtac cgggacctgt
     3121 ccggccaccc gctggtgatg atggtgctct gagggtgttt cgattgccgg cccgttccgc
     3181 gaccacgccg tgacgcccgc gatccgcgac ctcggccgcg ccgagtacga gcccgtgtgg
     3241 cgcgcgatgc aggcgttcac cacggcgcgc gacgattcca cgcccgacga actgtggttc
     3301 gtcgagcacc cgcccgtgtt caccctcggc ctcgcggcga agcccgagca cgtgctggac
     3361 gcgggagcca tcccggtcgt gcgcatcgac cgtggcggcc aggtcacgtg gcatgggccg
     3421 gggcagctcg tcgcctatgt ccttctcgac ctgaaacgtg ccggctacgg ggtaaaggaa
     3481 ctggtgcgcc gcctcgagca atcggtgatc gacctcctcg ccggctatgg agtcacgggc
     3541 gcccggcagg cgggaatgcc gggcgtctac gtgaacggcg ccaagatcgc cgccgtcggg
     3601 cttcgcgttt cgcgcggctg cagctaccac ggcatctcgc tcaacgtgga cgcggatctc
     3661 gcggcattct cgcgcatcaa cccctgcggc tacccggggc tcgcctccac gcgcatggcc
     3721 gaccacgggg tgcgtgatag catggaattc gtgcacgcgc gcctcgccga cgccctcgag
     3781 aaatgcctga caccccactg aagatccagc agaagcgcgg cct
//
LOCUS       DASUEV010000149         5743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1532720, whole genome shotgun sequence.
ACCESSION   DASUEV010000149 DASUEV010000000
VERSION     DASUEV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532720"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VFV55_04860"
     CDS             <1..1472
                     /locus_tag="VFV55_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX4943660.1"
                     /translation="EAQRIRLAAQLGSNLRGVCYILDEPTIGLHPRDNRILLETLRRL
                     GAKGNTLVVVEHDEDTIRHAEHVVDLGPGAGRLGGYIVAQGTADELQANPDSITGRFL
                     ASPLRHPVQPRRGVGPKVSAITVAGASLHNLKDIVARIPLARLSVVTGVSGSGKSTLA
                     RDVLHENLARLVAAARDRKAAPALLGCTSIRGWETVTRVLEVDQTPIGKTPRSCPATY
                     VGFFDTIRKLFADTQESRIRGYTASRFSFNAAGGGKGKGKGKDKGKAASAGGRCDTCE
                     GQGVKKIEMSFLPDVKVVCESCGGARFNPETLAVHLRGKSIGDVLAMSVDEAVEYFAA
                     HPSIHHALRLLQDVGLGYLTLGQQSPTLSGGEAQRIKLVTELAKVRNVEAPGPRGPRP
                     GGQHTLYVLDEPTVGLHMADVEKLIRVLHRLVDAGNTVVLIEHNLDVIAEADWIVDLG
                     PEGGDGGGRVVLAAAPEAFLAPGASTHTAKALREFLVGH"
     gene            complement(1474..2001)
                     /locus_tag="VFV55_04865"
     CDS             complement(1474..2001)
                     /locus_tag="VFV55_04865"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX4943661.1"
                     /translation="MAGPGLLTRTLEARPMDALAFAPYGALIDTASAKPEFINDGSTA
                     RHSGLATLDLRGPDRDPVIGIYVARARQFPLAITKLECHGQAAQVFIPLGEQCFVVVV
                     APGQDAPDWGRIEAFITAPGQGVCLHRGCWHHGLVALGDGDRFAVIEGGNYRCDTTEV
                     ASPVPIRLLPVVPCC"
     gene            complement(1980..2714)
                     /locus_tag="VFV55_04870"
     CDS             complement(1980..2714)
                     /locus_tag="VFV55_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4943662.1"
                     /translation="MSETLLRLDRVTAGYDRDIDVLREVSLEVHAGLVSGLIGLNGAG
                     KSTVIKTVCGFLAPKSGAIEYLGASIAGIAPHRLIDHGICCIPQESSLFPYLSVEENL
                     LLPLQGRAKAFAGVMRKRHEEVLEIFPDLKRKTRAQAGDLSGGQQKQVEFAKAWLQGP
                     KLCLIDEPSIGLSPILAEEVFGWIEKFARAGMGVLLIDHNVRRVVKMSDHVHVLSLGR
                     IAASGRREDFAGDLHEQVKQWLGLDY"
     gene            complement(2707..3429)
                     /locus_tag="VFV55_04875"
     CDS             complement(2707..3429)
                     /locus_tag="VFV55_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4943663.1"
                     /translation="MSLLRMQGLTRHFGGVAAVEDLTFAVESGEILGLIGPNGSGKTT
                     ALNMLSGFLEPDRGEVWLDGEAVTGMTTVRLARRGLLRMFQMSRVFARVSAFDNLLTA
                     GLAMGLDEREAGRRAGALLDELTLSPVRYLDAGQLSGGQRKLLEFGMCFIEPPRIALL
                     DEPFAAVHPVMRETMASFIRRRNALGQTFLLVSHDMPIVVELCQRTVCMNAGRLIAMG
                     PTREVLQDHAVIEAYLGSTSHV"
     gene            complement(3426..4403)
                     /locus_tag="VFV55_04880"
     CDS             complement(3426..4403)
                     /locus_tag="VFV55_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4943664.1"
                     /translation="MIVHANANLWVNRLRTDFRAALVVVALLALAPLFVTTPYSLGIL
                     VVSIYFALLALGWNLLAGFTGQFSLAPAAFAMLGAYATALLDYHLKAPLAIGIPAAIV
                     GTALLGWLLGRVVLRLKGPYLSLTTLSFAEIARVVIGNSHEFTRGDQGLNVPTLFESR
                     VGYYYLFLAVLLATLVGLYRLLRGRAGRFWLAIRDDATGAESRGIDVIRYKTFAFALS
                     CAICGLAGSLYGTFSQLVSPELGLLLQTGLVISMVVIGGIGTLTGPLVGALLVYLASE
                     WLRGVGNIQMIVFAAAVIVFARFFRTGLWGLVTRPSAQEAPAHSAEGKP"
     gene            complement(4400..5290)
                     /locus_tag="VFV55_04885"
     CDS             complement(4400..5290)
                     /locus_tag="VFV55_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4943665.1"
                     /translation="MLAVDLFVNGLIFGLFYALMAVGLAMIFGVLKVVNFAHGEFYMI
                     GAYTYALVALKLDVSPWVALPLAAVAGAGMGWLVERTLMRPLYAGYGSWSLMKDEYAV
                     VVTFGLSLLLINLVDKVVGPYPFRGPPLIETARFAVGPVMLNGQKLIAAGISLALLVG
                     LALFIKRSLWGRQIQAVAQNRLGASLAGIDATRTTALIFAISGLLAALSGALLAPLIN
                     PAPDVGAFPAIKSYVIVVLGGMGSVWGAMAAAILLGVLESFFAVYVSYDYRDAFGLLV
                     LILVLIFRPQGLLGEKGREV"
     gene            complement(5304..>5743)
                     /locus_tag="VFV55_04890"
     CDS             complement(5304..>5743)
                     /locus_tag="VFV55_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX4943666.1"
                     /translation="ERVRERGHLLGEDVRVRIEAGQFIPGYWYAKAQRMRRRFADAVD
                     AAFGDSDALVCPTLRAPAPAIGASRVRIGAADYPLHTAVTQLTMPFNLSGLPAISIPW
                     KLSADGVPICLQVVGRRGADWRTLAVAHRLEAASPWSRLRAAR"
BASE COUNT         1018 a         1968 c         1991 g          766 t
ORIGIN      
        1 gagaagcgca gcgcatccgc ctcgccgcgc aactgggctc caacctgcgc ggcgtctgct
       61 acatcctcga cgagccgacc atcggcctgc acccgcgcga caatcgcatc ctgctcgaga
      121 cgctgcgccg cctgggcgcc aagggcaata cgctggtggt cgtcgagcac gacgaggaca
      181 ccatccgcca cgccgagcac gtggtcgacc tcggcccggg cgcggggcgg ctcggcgggt
      241 acatcgttgc gcagggcacc gcggacgaac tgcaggccaa tccggattcc atcaccggcc
      301 gattcctcgc cagcccgctg cggcacccgg tgcagccgcg acgcggggtc gggccgaagg
      361 tttccgccat cacggtcgcc ggcgcgagcc tgcacaacct gaaggacatc gtcgcacgca
      421 ttcccctcgc gcggctgtcc gtggtgacgg gcgtctccgg ttcgggcaag tcgacgctgg
      481 cccgcgacgt tctgcacgaa aacctcgcgc ggctggtcgc ggccgcgcgc gaccggaagg
      541 ccgcccccgc gcttttaggc tgcacgtcga tcaggggctg ggagacggtc acccgcgtgc
      601 tggaagtgga ccagacgccc atcggcaaga cgccgcgttc ctgcccggcc acctacgtcg
      661 gcttcttcga caccatccgc aagctcttcg ccgacacgca ggagtcgcgc atccgcggct
      721 acaccgcgag ccgcttctcc ttcaacgcgg cgggcggagg caagggcaag ggaaagggaa
      781 aggacaaggg aaaggcggcg tccgctggcg ggcgttgcga cacgtgcgaa ggccagggcg
      841 tgaagaagat cgagatgagc ttcctccccg acgtgaaagt ggtgtgcgaa tcctgcggcg
      901 gcgcgcgttt caaccccgag accctggcgg tgcacctgcg cggcaagtcg atcggtgatg
      961 tcctggccat gagcgtggac gaagccgtcg agtacttcgc ggcgcatccc tcgatccacc
     1021 acgccctgcg cctgcttcag gacgtggggc tgggctacct caccctcggc cagcagagcc
     1081 cgacgctatc cggcggcgag gcgcagcgca tcaagctggt gacggagctt gccaaggtgc
     1141 ggaacgtcga ggccccgggc ccgcgcggac cgcggccggg cgggcagcac acgctctacg
     1201 tcctcgacga gcccaccgtg ggcctgcaca tggccgacgt cgagaagctc atccgcgtgc
     1261 tgcatcgcct cgtggacgcg ggcaacaccg tcgtcctgat cgagcacaac ctcgacgtga
     1321 tcgcggaagc cgactggatc gtggacctcg gtcccgaggg cggcgacggc ggcggacgcg
     1381 tcgttctggc ggcggcgccg gaagcgtttc tcgcccccgg cgcaagtacg cacactgcga
     1441 aggcgctgcg cgagttcctc gtggggcatt gaatcaacag cagggaacca cagggagaag
     1501 ccggatcggt accggggacg caacttcagt cgtgtcgcat cggtagttcc cgccctcgat
     1561 caccgcgaag cggtcgccgt cgcccagcgc caccagcccg tgatgccagc agccgcggtg
     1621 caggcacacg ccctggccgg gtgcggtgat gaacgcctcg atgcgacccc aatcgggcgc
     1681 atcctgtccc ggcgcgacca ccaccacgaa acattgctct cccagcggaa tgaagacctg
     1741 cgccgcttgc ccgtggcatt cgagcttcgt gatcgcgagc ggaaactgcc gcgcccgggc
     1801 aacgtagatt ccgatcaccg ggtcgcggtc gggcccgcgc aggtcgagcg tcgcgaggcc
     1861 ggaatggcgc gccgtgctgc cgtcgttgat gaattccggc ttcgcggacg cggtgtcgat
     1921 gagcgctccg tagggggcga acgcaagcgc gtccatgggc cgcgcctcca gcgtgcgcgt
     1981 cagtaatcca ggcccagcca ctgcttcacc tgctcgtgca ggtcgcccgc gaaatcctcg
     2041 cgccgcccgg acgcggcgat gcgcccgagg ctcaggacgt gcacgtgatc cgacatcttc
     2101 accacgcggc gcacgttatg gtcgatgagg agcacgccca tgccggcacg ggcgaacttc
     2161 tcgatccagc cgaagacctc ctcggcgagg atgggcgaga gcccgatgct cggctcgtcg
     2221 atgaggcaga gcttcggccc ctgcagccag gccttggcga actccacctg cttctgctgc
     2281 ccgccggaaa ggtccccggc ctgcgcgcgc gtcttgcgct tgaggtccgg gaagatctcg
     2341 agcacttcct cgtggcgctt gcgcatcacc ccggcaaagg ccttcgcccg gccctggagc
     2401 ggcaggagca ggttttcctc gacgctcagg tagggaaaca ggctcgactc ctgcgggatg
     2461 cagcagatgc cgtggtcgat gaggcggtgc ggggcgatgc cggcgatcga ggcaccgagg
     2521 tattcgatgg cgccggactt cggggcgaga aatccgcaga ccgtcttgat caccgtggac
     2581 ttgcccgcgc cgttgaggcc gatgagcccg ctcaccaggc ctgcgtgaac ctcgagggac
     2641 acctcgcgca gcacgtcgat gtcgcgatcg tagccggcgg taacgcggtc gaggcgaagg
     2701 agcgtctcag acatggctcg tgctgcccag gtaggcctcg atcaccgcgt ggtcctggag
     2761 cacctcgcgc gtggggccca tcgcgatcag gcgcccggcg ttcatgcaca ccgtgcgctg
     2821 gcaaagttcc accacgatgg gcatgtcgtg gctcacgagc aggaacgtct gcccgagcgc
     2881 attgcgccgg cgaatgaagg aggccatggt ctcgcgcatc accgggtgca cggcggcgaa
     2941 gggctcgtcg aggagcgcga tgcgcggcgg ctcgatgaag cacatgccga actcgaggag
     3001 cttcctctgc ccgccggaaa gctggcccgc gtcgagatag cgcacgggcg agagcgtgag
     3061 ctcgtcgagg agcgcgccgg cgcgccgccc cgcctcgcgc tcgtcgagcc ccatggcgag
     3121 gcccgcggtg agcaggttgt cgaaggcgga gacccgggcg aatacccgcg acatctggaa
     3181 catccgcagc agcccccggc gcgcgagccg caccgtcgtc atgcccgtca cggcctcgcc
     3241 gtccagccac acctcgcccc ggtccggctc gaggaacccg gaaagcatgt tgagcgcggt
     3301 cgtctttccc gatccgttgg gcccgatgag gccgaggatt tcgccggact cgaccgcgaa
     3361 agtaaggtcc tcgaccgcag ccacgccgcc gaagtgcctg gtgaggccct gcatgcgcag
     3421 caggctcacg gcttgccctc cgccgagtgc gccggcgctt cctgcgcact cggcctcgtg
     3481 acgagacccc acagcccggt gcggaagaat cgcgcgaaga cgatcacggc cgcggcaaag
     3541 acgatcatct ggatgttgcc gacgccgcgc agccactcgg aggcgaggta gacgaggagc
     3601 gcgcccacga gagggcccgt cagcgtgccg atgccgccga tcaccaccat cgagatcacc
     3661 aggcccgtct gcagcagcag ccccagttcg gggctcacca gctggctgaa ggtcccgtag
     3721 agggatccgg cgagcccgca gatcgcgcac gacagggcga aggcgaacgt cttgtagcgg
     3781 atcacgtcga tgccgcggct ttccgccccg gtggcgtcat cgcggatcgc cagccagaag
     3841 cgtcccgcgc ggccccgcag gagccggtac aggccgacga gcgtggcgag caggacggcg
     3901 aggaacaggt agtagtaccc gacgcggctc tcgaagagcg tgggcacgtt gaggccctgg
     3961 tcgccgcgcg tgaactcgtg cgagttgccg atgaccacgc gcgcgatctc ggcgaaggag
     4021 agcgtggtga gcgagaggta cggccccttg aggcgcagca ccacgcggcc cagcaaccag
     4081 cccagcagcg cggtccccac gatcgccgcg ggaatgccga tggccagcgg cgccttcagg
     4141 tggtagtcga gcagcgcggt cgcataggcg cccagcatcg cgaaggcggc gggtgcgaga
     4201 gagaattgcc ccgtgaatcc ggcgagcagg ttccagccga gggccagcaa cgcgaagtag
     4261 atgctgacga cgaggatgcc gagggaataa ggcgtggtca cgaacagcgg cgccagcgcg
     4321 agcaacgcca ccaccacgag cgcggcgcgg aaatccgtgc gcaggcggtt gacccagagg
     4381 ttggcgttgg cgtgcacgat catacttccc gccccttctc gccgaggagc ccctgcggcc
     4441 ggaagatcag cacgaggatg aggaccagca ggccgaaggc gtcgcgatag tcgtaggaca
     4501 cgtacaccgc gaagaacgac tcgagcacgc cgaggaggat cgcggccgcc atcgcccccc
     4561 acaccgaccc catgccgccg agcacgacga tcacgtacga cttgatcgcc ggaaaggcac
     4621 ccacgtcggg cgcgggattg atgagcggcg cgagcaacgc cccggagagc gcggcgagca
     4681 ggccggagat cgcgaagatg agggccgtgg tgcgcgtggc gtcgatgccc gcgagcgatg
     4741 cgccgaggcg attctgcgcc acggcctgga tctgccgccc ccacagcgag cgcttgatga
     4801 acagcgcgag gcccaccagg agcgcaagcg agatccccgc ggcgataagc ttctggccat
     4861 tgagcatcac cggacccacg gcgaagcggg cagtctcgat gagcggcggg ccacggaaag
     4921 gatacggccc gacgaccttg tcgacgaggt tgatgagcag cagcgacagg ccgaaggtga
     4981 ccacgaccgc gtattcgtcc ttcatcaggc tccagcttcc gtagcccgcg taaagcggcc
     5041 gcatcagcgt gcgctccacg agccagccca tgcccgcgcc cgcgacggcc gcaagcggaa
     5101 gcgcgaccca cggcgagacg tccagcttca gggcgacgag ggcgtaggtg taggcgccga
     5161 tcatgtagaa ctcgccgtgc gcgaagttca ccaccttgag aacgccgaag atcatggcga
     5221 gccccacggc catcagcgcg tagaagagtc cgaagatgag gccgttgacg aagagatcga
     5281 ccgcgagcat ggggtgcctg cgcctacctc gccgcccgta ggcgggacca gggcgaagcc
     5341 gcttcgagcc ggtgcgccac ggcaagggtg cgccagtcgg cgccgcggcg gccgaccacc
     5401 tggaggcaga tcggcacgcc gtccgccgac agcttccagg gaatggagat cgcgggcagc
     5461 cccgagagat tgaacggcat cgtcaactgc gtcacggcgg tgtgcagggg gtagtcggct
     5521 gcgccgatgc gcacgcgact cgcgccgatc gccggcgcgg gcgcacgcag cgtcgggcaa
     5581 acgagcgcat ccgaatcgcc gaacgcggca tcgacggcat ccgcgaacct gcggcgcatg
     5641 cgctgcgcct tggcatacca gtaacccggg atgaactgcc cggcctcgat gcgcacgcgc
     5701 acgtcctcgc cgaggagatg gccgcgctcg cgaacacgtt ccg
//
LOCUS       DASUEV010000150        10446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1074830, whole genome shotgun sequence.
ACCESSION   DASUEV010000150 DASUEV010000000
VERSION     DASUEV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074830"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..327
                     /locus_tag="VFV55_04895"
     CDS             160..327
                     /locus_tag="VFV55_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX4943667.1"
                     /translation="MDINAIRSGITVVALITFLAIVAWAWSSRRRADFEAAARLPLDE
                     EEDAIPREKQR"
     gene            324..1238
                     /gene="ccoP"
                     /locus_tag="VFV55_04900"
     CDS             324..1238
                     /gene="ccoP"
                     /locus_tag="VFV55_04900"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX4943668.1"
                     /translation="MSDFVDGFWSLYIAGITLVSIVACALLLTLQHKRRPAGQVVDTT
                     GHQWDEDLAEFNNPLPKWWIWLFWITIVFSLLYLVLYPGLGSFKGVLGWSSTGQYKAE
                     QASADATYGPVFQKYAAMDIEAIAKDPKARVMGERMFLTYCSQCHGSDARGAKGFPNL
                     ADNDWLWGGEPQTILTTIMEGRNGMMPPMGAAVGGPEGVREVANYVLSLSGATHDAKL
                     AEAGKAKFAACAACHGPEAKGNPALGAPNLTDKTWLHGGSLAAISEQIDKGRTNVMPA
                     HKDFLGPERSRILAAYVWSLSQAPAGAK"
     gene            1235..2719
                     /gene="ccoG"
                     /locus_tag="VFV55_04905"
     CDS             1235..2719
                     /gene="ccoG"
                     /locus_tag="VFV55_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX4943669.1"
                     /translation="MTRTVAEARPGTGAAPTIALKAIVSAAQEETERGLFEVRRKIYP
                     RAVSGWFATWRWALVWFTQILFYGLPWVAWNDRQAVLFDLGARKFYIFGLVFWPQDFI
                     YLTLLLVAAAVSLFLFTAVAGRLWCGYACPQTVYTEIFLWIERVIEGEPNARTKLDAA
                     PLTARKFWLKAAKHGTWAALAFWTGFTFVGYFSPVKVLGGDVLSFSLGPWETFWIFFY
                     GFATYGNAGWMREQVCMYMCPYARFQGAMFDQDTLIIAYDGGRGDPRGSRSKKADPKA
                     LGLGDCVDCGICVQVCPTGIDIREGLQYECIGCAACIDGCDKVMEKMGYPKGLIRYST
                     EHALQERSDLAGILKRFFRPRTLVYTTILATVVVAAAAALFVRVPLKVDVIRDRGAMV
                     REVGDGELENVYRLQVMNATESARRFRITVEGLHGVRVASEPVVEMAAASTRAFPVRI
                     RLSEEHAKGGSQKFWITVAAEDGSGIQVREKAVFLSPHKDKDEH"
     gene            2725..3234
                     /locus_tag="VFV55_04910"
     CDS             2725..3234
                     /locus_tag="VFV55_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX4943670.1"
                     /translation="MTATSHRHPPRKPWYREPWPWLLMSGPAIVVVAGIATAVIAFRG
                     ADGLVADDYYKQGLTINRQIARDQMAQELGISGELRLLPGIVRVSLRSTAALPDRMTL
                     RLVHPARASEDRVLYLARTADGAWEAPLAGPLPAVHWRAIVETARWRVAAQFESKSAE
                     AAAFSPGVR"
     gene            3249..3545
                     /locus_tag="VFV55_04915"
     CDS             3249..3545
                     /locus_tag="VFV55_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943671.1"
                     /translation="MLTQRIAWIVWPAFLVACAMEVVFFTLFDPADMSFFGASLEASR
                     MAIYSVGFLAFWAMGAASSFLTCFLQRSPWEVNRCPLPPTKRPDGCPKREDSPC"
     gene            complement(3576..6344)
                     /locus_tag="VFV55_04920"
     CDS             complement(3576..6344)
                     /locus_tag="VFV55_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEX4943672.1"
                     /translation="MSIKLRNRLAARRFRIPDAAAVPAAPGISKLVHPLHPLLSPGSV
                     ALVGASERPGTLGQVVLTNLRQGGFRGPIHLVNPRYSELNGDPCYASLENLPKPVDLA
                     IVAAPAPAVPDIIAQAGRAGVPAVVVLSAGFSETGDAGKELEARVLAVARESDVRILG
                     PNCIGVLRPSIGLNASFCRTACEAGSIALVSQSGAICTALVDWAASTKVGLSSVVSLG
                     GAADVDFGDVLDYLLFDPRTESVLLYVEGIHDGRTFISSLRAIARAKPVIVLKVGRYA
                     SGSQAARSHTGALVGNDAVFDAVLARCGVVRVHSAQDLFATARALSSRRKPHGDRLAV
                     VTNGGGPGVLAADACSSENVQLAKLSPETIEKLNAILPKHWSHANPVDVIGDADGERF
                     GGAAKIVADDPNVDAVLTAFCPTGIASAESVADGILPVARECSKPFLTAWLGESSVVA
                     TRHRVEEAGVPAYRAGEVAVQAFAALATHVKNQRLLLESPPPRSTTVEHDIARAEEIF
                     QVAVDQGRTLLDEVEAKSLLSAFGIPVPPFAIATTREEAVTAAQTIGYPVVLKILSPD
                     VSHKSDVNGVRLSVRDAEAVGKQFDDIVETVGRMRPEARITGVLVQAMVARRDGRELM
                     IGVMTDPAFGPVISFGSGGVAVELLKDSAVGLPPLNIRLASDLVDRTRAARLLGAYRN
                     VPAANRDALFDVLLRVSDLVCALPWVAEMDINPLLLDPTGAVALDARVVVDPNRRHLD
                     SRYSHLAIHPYPEALEAVERLRDGTEVTIRPIRPEDAERETAFITKLSDESRYMRFLS
                     IVRHVTPEMVARFTQIDYDREMALIATRKGGDGESIIGVARYVRDPNPASAEFAVVIA
                     DRAQRKGLASKLMGRLMAHARFAGIERLRGLVLASNDSMLELMDRLGFEAFPVPDDPS
                     LVDVLKIL"
     gene            complement(6354..6761)
                     /locus_tag="VFV55_04925"
     CDS             complement(6354..6761)
                     /locus_tag="VFV55_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX4943673.1"
                     /translation="MGADSGMNGLDARQLGRLRAELTRLKGEAMAGVRRAAGELSGED
                     ADLFRDLGLTGDGAVAEAEFERDVAGAGQVRAALISIVGAERRLEKGEYGLCEDCGEA
                     IGFARLEAQPAASRCVRCQELAERPGGPTHFPG"
     gene            complement(6761..7204)
                     /locus_tag="VFV55_04930"
     CDS             complement(6761..7204)
                     /locus_tag="VFV55_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4943674.1"
                     /translation="MFRRILCATDGSKRSTHAVKTAAALAHGSGALLTLVHVTPDYRT
                     PYYPDGVMIDWPSEKDYKRDCKAAADKVFAKDLATALKEGAEAETLQLFGDSPADEIL
                     GAVRKLKADLIVMASHGRKGFEALLLGSETQKVLARTSVPVLVVR"
     gene            complement(7313..8053)
                     /gene="fnr"
                     /locus_tag="VFV55_04935"
     CDS             complement(7313..8053)
                     /gene="fnr"
                     /locus_tag="VFV55_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEX4943675.1"
                     /translation="MTEPALATLTRLRTACSQCNLRELCLPMGLSSDELQTLDNLVST
                     RRKVRRGDSLFYGGEPFSALYAVRYGFFKSTLTSGDGREQVTGFYMAGELLGMDGIGA
                     DRHTCTLTALEDSEVCVMPFARVEDLSRNFEPLQRHFHKVLSREIVRDQGVMMLLGSM
                     RAEERLAAFLVNLSQRLTTRGFSASEFVLRMTRDEIGSYLGMKLETVSRLFSRFQEDN
                     FIEVHQKHVRILDIGGLKRVMAQAQNVP"
     gene            8141..9574
                     /gene="hemN"
                     /locus_tag="VFV55_04940"
     CDS             8141..9574
                     /gene="hemN"
                     /locus_tag="VFV55_04940"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX4943676.1"
                     /translation="MLSVVTQNTTTPTPRPESLPAQIDLDLVQRFDKLGPRYTSYPTA
                     DRFVEAFDAQAFQRAMSTRLIGAVRQPLSLYVHLPFCNTICFYCGCNKVVTRDKSRAA
                     RYLHYLGVEIAMQAALAGDDRRVRQMHWGGGTPTFLSDHELRVVWELLRSNFEFSPDG
                     EYAIEIDPRSCSPDTIRLLAELGFNRASFGVQDFDPAVQRAVNRIQPYEMTRDVIDAS
                     RASGFKSLNVDLIYGLPKQSVSSFADTVERVLELRPDRIALYSYAHLPQRFKPQRRIL
                     DVDLPSAAEKLSIMARAIDLFNRAGYIYIGMDHFALPDDELARAQRCGTLTRNFQGYT
                     VGPDGDMLSFGISAIGKVGPTYSQNARDLPAYYDTLEQGRLPVIRGIELSADDLLRRA
                     IIVALMCQFCVSIESIEAAHLVKFDDYFAFEIEALKEFEELGLVEREPGWIAVTPKGR
                     FFVRAICAVFDRYLRADKARVAYSRII"
     gene            9597..10319
                     /locus_tag="VFV55_04945"
     CDS             9597..10319
                     /locus_tag="VFV55_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX4943677.1"
                     /translation="MTGALALSAMLLAVYGAAHCAAMCGGFVASTFTPASGSPARPAV
                     LHRAALPMHAGRLLAYAAAGALAGALGAAPAVFLGSARYQAVLFSVGALVLVVTGLRI
                     AGIRLVRPANALLAQPLWNWAANEARRLGAPDTLPKRLGLGVLWGCAPCAIVYSALPL
                     ALASGSATGGALVMLAFGAGTLPALVAAGWLIGRGEALFQGRTARRVAGLLIVVLALA
                     GIAQALGVADTALGSFCVSAPH"
BASE COUNT         1629 a         3639 c         3484 g         1694 t
ORIGIN      
        1 tgccggcgaa gatgcgggca ctgcgcatgg tcggggtgcc gtacgccgat gcggagatcg
       61 cctcttccac caaggacgtc gccggcaaga ccgaggagga cgcgctcatc gcctacctcc
      121 aggaactcgg caccgtcatc aagtcgcgga ggtagcgcca tggacatcaa cgcaatccgc
      181 tccggcatca ccgtggtcgc cctgatcacc ttcctcgcga tcgtcgcctg ggcctggtcc
      241 tcgcgccgcc gcgccgactt cgaagcggcc gcccgcctgc ctctggacga ggaggaggac
      301 gccatcccca gggagaagca acgatgagcg atttcgtcga cggtttctgg agcctctaca
      361 tcgccggcat cacgctggtg tcgatcgtcg cctgcgcgct gctgctcacc ctgcagcaca
      421 agaggcgccc cgcgggccag gttgtcgaca ccaccggcca ccagtgggac gaggacctcg
      481 cggagttcaa caacccgctg cccaagtggt ggatatggct cttctggatc acgatcgtct
      541 tctcgctgct ctacctcgtc ctctacccgg ggctgggttc attcaagggc gtgctcgggt
      601 ggtcctcgac cggccagtac aaggccgagc aggcgagcgc cgacgcgacg tacggtccgg
      661 tgttccagaa gtacgccgcg atggacatcg aggcgatcgc gaaggatccg aaggcaaggg
      721 tcatgggcga gcgcatgttc ctcacctact gctcgcaatg ccacgggtcg gacgcgcgcg
      781 gggcgaaggg attcccgaac ctcgcggaca acgactggct gtggggcggg gagccgcaga
      841 ccatcctcac gaccatcatg gagggccgca acggcatgat gcctccgatg ggtgccgcgg
      901 tcggcgggcc cgaaggcgtg agggaagtcg cgaactacgt gctgtccctg tcgggcgcga
      961 cgcatgacgc gaagctcgcg gaagcaggca aggcgaagtt cgccgcctgc gcggcctgcc
     1021 acgggccgga ggccaagggc aatcccgccc tgggcgcacc caacctcacc gacaagacct
     1081 ggctgcatgg cgggagcctc gcggcgatct ccgagcagat cgacaagggc cgcacaaacg
     1141 tcatgccggc gcacaaggac ttcctcggtc cggagcgctc gcggattctc gccgcctacg
     1201 tctggagcct ttcgcaggcg ccggccgggg cgaagtgacg cggacggtgg ccgaggcgag
     1261 accggggacg ggtgccgccc ccaccatcgc gctcaaggcc atcgtgtccg cggcgcagga
     1321 agagaccgag cgcggtctct tcgaggtccg caggaagatc tatccgcgcg cggtaagcgg
     1381 ctggttcgcc acctggcgat gggccctcgt gtggttcacg cagatcctct tctacggcct
     1441 gccgtgggtc gcgtggaacg acaggcaggc ggtcctcttc gacctcggcg cgcgcaagtt
     1501 ctacatcttc ggcctcgtct tctggccgca ggatttcatc tacctcacgc tgctgctcgt
     1561 cgcggcggcg gtgtccctct tccttttcac cgccgtggcc ggcaggctct ggtgcgggta
     1621 cgcctgcccg cagaccgtct acacggagat cttcctgtgg atcgagcggg tgatcgaggg
     1681 cgaaccgaat gcccgcacca agctcgacgc ggccccgctc accgcgcgca agttctggct
     1741 gaaggccgcc aagcacggca cctgggcggc gctcgccttc tggaccggct tcactttcgt
     1801 gggctacttc tcgcccgtca aggtcctggg cggcgacgtg ctctccttct ccctcggtcc
     1861 gtgggagacc ttctggatat tcttctacgg gttcgccacc tacggcaacg ccggctggat
     1921 gcgcgagcag gtgtgcatgt acatgtgccc ctatgcgcgc ttccagggcg ccatgttcga
     1981 ccaggacacg ctcatcatcg cctacgacgg cgggcgaggc gatccgcggg gatcgaggag
     2041 caagaaggcc gacccgaagg ccctcggcct cggcgattgc gtcgactgcg gcatctgcgt
     2101 ccaggtctgc ccgaccggca tcgatattcg cgagggcctg caatacgagt gcatcggctg
     2161 cgccgcgtgc atcgacggct gcgacaaggt gatggagaag atgggctacc cgaagggact
     2221 catccgctac tcgaccgagc atgcgctgca ggagcgcagc gacctcgccg ggatcctcaa
     2281 gcgcttcttc cggccccgga ccctcgtgta caccacgatc ctcgcgacgg tcgtcgtggc
     2341 cgccgccgcc gcgctgttcg tgcgcgtgcc gctgaaggtc gatgtgatac gcgatcgcgg
     2401 cgcgatggtg cgtgaggtcg gggacggcga actcgagaac gtctaccgcc tgcaggtgat
     2461 gaacgccacc gaatcggcgc gacgcttccg catcacggtc gaggggcttc acggcgtgcg
     2521 cgtggcctcc gagcccgtcg tcgagatggc cgccgcctcc acgcgcgcct tcccggtgcg
     2581 catccggctg tcggaagagc acgcgaaagg ggggtcgcag aagttctgga tcacggtagc
     2641 ggccgaagac ggcagcggca tccaggtccg cgaaaaggcc gtgttcctct cgccacacaa
     2701 ggacaaggac gagcattgaa cgccgtgacc gctacatcac accgtcatcc gccgcgcaag
     2761 ccctggtacc gcgagccctg gccgtggctc ctcatgtccg ggccggccat tgtcgtggtc
     2821 gccggcatcg cgaccgccgt catcgccttt cgcggcgccg acggcctggt ggccgacgac
     2881 tactacaagc aggggctcac catcaaccgc cagatcgccc gcgaccagat ggcgcaggaa
     2941 ctcggcatca gcggcgaact gcggctcctg ccgggcatcg tccgcgtgtc actgcgctcc
     3001 acggccgcgc tccccgatcg catgacgctg cgcctggtcc atcccgcacg cgcttcggaa
     3061 gaccgcgtcc tgtacctcgc acgcaccgcc gacggcgcct gggaggcgcc gctcgcgggc
     3121 ccccttcccg ccgtccactg gcgcgccatc gtcgagacgg cacgatggcg ggtcgccgca
     3181 caattcgaat cgaaatccgc cgaagcggct gcgttctccc ccggcgtacg ctgaaaagga
     3241 aacgaatcat gctcacgcaa cgcatcgcct ggattgtctg gccggcgttc ctcgtcgcct
     3301 gcgccatgga ggtcgtgttc ttcaccctct tcgacccggc agacatgagc ttcttcgggg
     3361 cgagcctcga ggcgagccgc atggccatct actccgtcgg attcctcgcc ttctgggcga
     3421 tgggcgcggc ctccagtttc ctcacctgct tcctgcagcg ctcgccgtgg gaagtgaacc
     3481 gctgccccct gccccccacg aagcggcccg acggctgccc gaagcgcgaa gactctccct
     3541 gctgaggccg aagcgccgca gccgccccgc gaaggctaga ggatcttcag gacgtcgacg
     3601 aggctcgggt cgtcgggcac cgggaacgcc tcgaagccga gcctgtccat caactcgagc
     3661 atcgagtcgt tggatgccag cacgaggccg cgcagccgct cgatgcccgc gaagcgcgcg
     3721 tgcgccatga gccggcccat gagcttcgag gcgagcccct tgcgctgggc gcggtcggcg
     3781 atgacgacgg cgaactccgc gctcgccgga ttgggatcgc gcacgtagcg tgccaccccg
     3841 atgatcgact cgccatcgcc ccccttgcgc gtggcgatga gagccatctc gcggtcgtag
     3901 tcgatctgcg tgaaacgcgc caccatttcc ggcgtcacgt ggcgcacgat cgagaggaaa
     3961 cgcatgtagc gcgattcgtc cgaaagcttg gtgatgaacg cggtttccct ctcggcgtcc
     4021 tcggggcgga tcgggcggat cgtcacttcc gtcccgtcgc gcaggcgctc gacggcttcc
     4081 agcgcctcgg gatagggatg gatcgccagg tgcgaatagc ggctgtccag gtgccggcgg
     4141 ttcggatcca cgacgacccg cgcgtcgagc gcgacggccc cggtcgggtc gaggaggagc
     4201 gggttgatgt ccatctcggc gacccacgga agggcgcaga cgaggtccga gacgcgcagc
     4261 agcacgtcga aaagggcgtc gcggttggcg gcaggtacgt tgcggtaggc cccgagcagc
     4321 ctcgcggcac gcgtccggtc gaccaggtcg ctggccagcc ttatgttgag cggcggaagc
     4381 cccaccgcgc tgtctttcag cagctcgacg gccacgccgc ccgaaccgaa gctgatgacg
     4441 ggcccgaagg cagggtcggt catcacgccg atcatgagtt cgcgcccgtc cctgcgggcg
     4501 accatggcct gcacgagaac gccggtgatg cgcgcttccg gtcgcattct ccccacggtc
     4561 tcgacgatgt cgtcgaactg cttgcccacg gcctcggcgt cgcgcacgct gaggcgcacg
     4621 ccattcacgt cggacttgtg cgagacgtcc ggcgaaagga tcttcagcac cacgggatag
     4681 ccgatcgtct gcgcggcggt gaccgcttcc tcgcgcgtcg tggcgatggc gaagggtgga
     4741 acggggatcc cgaaggcgga gagcagggac ttcgcctcca cttcatcgag aagcgtgcgc
     4801 ccctggtcca cggcgacctg gaagatctcc tcggccctcg cgatgtcgtg ttccaccgtc
     4861 gtcgaacgag gcggcggcga ctcgaggagc agcctctggt tcttcacgtg cgtggcgagg
     4921 gccgcgaatg cctggacggc gacctccccg gcgcggtacg ccggcacgcc cgcctcttcc
     4981 acgcgatggc gcgtggcgac cacgctcgac tccccgagcc aggcggtgag gaatggcttg
     5041 gagcattccc tggccacggg caggattccg tcggcgacgg attccgcgct cgcgatgccg
     5101 gtcgggcaga acgccgtgag gacggcgtcc acgttgggat catccgccac gatcttcgcg
     5161 gcgccgccga atcgctcgcc gtcggcgtcc ccgatcacgt cgacgggatt ggcatgcgac
     5221 cagtgcttcg gcaggatcgc gttgagcttc tcgatcgtct cgggggaaag cttcgcgagc
     5281 tgcacgttct cggaactgca cgcgtcggcg gcgagcacgc ccgggccgcc cccgttggtg
     5341 accacggcga gccggtcgcc gtggggcttg cgccgcgagg acagcgcgcg cgcggtcgcg
     5401 aacaggtcct gggcgctgtg cacgcgcacc acgccgcaac gggcgagcac ggcgtcgaat
     5461 accgcgtcgt tgccgaccag ggcaccggtg tgcgagcgcg ccgcctggga gcccgaggca
     5521 tagcgcccga ccttcagcac gatgaccggc ttggcccgcg cgatggcgcg caggctgctg
     5581 atgaaggtcc ggccgtcgtg gatgccctcg acgtagagca gcacgctttc cgtcctggga
     5641 tcgaagagaa ggtagtcgag gacgtcaccg aaatcgacgt cggccgcgcc cccgagggag
     5701 acaacgctcg acagcccgac cttggtgctg gccgcccagt cgacgagcgc cgtgcagatc
     5761 gcgcccgact gcgacaccag cgcgatgctg cccgcctcgc aggcggtccg gcagaacgag
     5821 gcgttgagcc cgatggacgg gcgcaggacg ccgatgcagt tcggcccgag gatccgcacg
     5881 tcggattcgc gcgccaccgc gagcacccgc gcttcgagct ccttgccggc atcgcccgtc
     5941 tcgctgaatc cggcagagag cacgacgacg gccggaacgc cggcccgtcc ggcctgcgcg
     6001 atgatgtcgg gcacagcggg ggcaggggct gcgacgatcg cgaggtccac gggcttgggc
     6061 aggttctcca gggacgcgta gcagggatcg ccgttcagct cgctgtaccg cggattcacc
     6121 aggtggatcg gcccgcggaa gcccccctgg cgcaggttcg tgagcaccac ttggccgagg
     6181 gtaccgggac gttcgctcgc gcccaccagg gctaccgagc cgggcgacag caggggatgc
     6241 aggggatgga ccagcttcga gatccccggc gcggccggaa cggctgccgc gtcgggaatc
     6301 cggaagcggc gggcggcgag acggttcctc agcttgatgc tcatgtttgc ggcttatccg
     6361 ggaaagtgag ttggcccgcc cggcctttcg gccagttcct ggcagcggac acatcgcgaa
     6421 gcggcaggct gcgcttccag gcgtgcgaat ccgatggcct ctccgcaatc ctcgcaaagg
     6481 ccgtactcgc ccttctccag gcggcgctct gcacccacga tagagatgag cgccgcgcgg
     6541 acttgccccg cgccggccac gtcgcgctcg aactccgctt ccgcaacggc cccgtcgccc
     6601 gtcaggccga gatcgcggaa gagatccgca tcctctccgc tcaactcgcc ggcggcacgc
     6661 cgcactccgg ccatggcctc gcccttgagg cgcgtcagtt cggcgcgcag gcgaccgagc
     6721 tggcgggcat ccaggccgtt catgccggag tcggcgccca tcaacgcacc acgagcacgg
     6781 gtacggacgt gcgcgcgaga accttctgcg tctcgctgcc gagcagcagg gcctcgaatc
     6841 ccttgcggcc gtgcgaggcc atgacgatca ggtccgcctt cagcttcctc acggcgccga
     6901 gtatctcgtc ggccggcgag tcgccgaaga gctgcagcgt ttcggcttcg gcgccctcct
     6961 tcagggccgt cgcgaggtcc ttggcgaaca ccttgtcggc tgcggccttg cagtcgcgct
     7021 tgtagtcctt ttccgacggc cagtcgatca tcacgccgtc ggggtaatag ggcgtgcggt
     7081 agtcgggcgt cacgtggacg agcgtcagga gcgcgccgga tccatgcgcc agggctgcgg
     7141 ccgtcttcac ggcgtgggtc gagcgcttgg aaccgtccgt ggcgcagaga atgcgtctga
     7201 acatggcagg tctccgctgg aggggaatgg gacttcgtga aatccatgct accccgggga
     7261 cccccgcctc ggttgaccgg gatcaagcac ggctcgacgc gccggaaagc gctcagggca
     7321 cgttctgcgc ctgggccatc acccgcttca ggccaccgat gtcgagtatc cggacgtgct
     7381 tctggtggac ctcgatgaag ttgtcctcct ggaagcgcga gaacaggcgg ctcaccgtct
     7441 cgagcttcat cccgaggtag ctgccgatct cgtcgcgcgt catgcgcagg acgaactccg
     7501 aggcggagaa tccacgcgtg gtgagccgct gcgaaaggtt caccaggaat gccgcgaggc
     7561 gctcctcggc gcgcatcgat ccgagcagca tcatcacgcc ctggtcgcgc acgatctcgc
     7621 gtgagagcac cttgtggaaa tggcgctgca gcggctcgaa gttccgcgag aggtcctcga
     7681 cacgggcgaa gggcatcacg cacacctcgc tgtcttccag cgcggtgagc gtgcacgtat
     7741 gacggtccgc gccgattcca tccatgccga ggagctcgcc cgccatgtag aacccggtga
     7801 cctgctcgcg gccgtcaccg ctggtgagcg tgctcttgaa gaagccgtag cgcacggcgt
     7861 acagggcgga gaacggctcg cccccgtaga acaggctgtc gccgcgccgc accttgcgtc
     7921 gcgtcgaaac caggttgtcg agggtctgca gttcatccga gctgagtccc atcggaaggc
     7981 acaattcccg caggttgcac tgcgagcacg cggtgcgcag gcgcgtgagc gtggcgaggg
     8041 ctggctcggt catggggtac attgtagacg gcaggaaagc gcccggcgag gcttgatcca
     8101 tgtcaaggcg ccagtctgcc attgacggaa gatccgatcc atgctctccg tcgtgaccca
     8161 gaacaccacc acgcccacgc cgcgcccgga atcgctgccg gcgcagatcg atctcgacct
     8221 ggtgcagcgc ttcgacaagc tgggcccgcg ctacaccagc tacccgacgg cggaccgctt
     8281 cgtcgaggca ttcgatgcgc aggccttcca gcgggccatg agcacgcgcc tgatcggcgc
     8341 cgtgcgccag ccgctgtcgc tctacgtgca cctgccgttc tgcaacacca tctgcttcta
     8401 ttgcggctgc aacaaggtgg tcacgcgcga caagtcccgc gctgcgcgct acctgcacta
     8461 cctcggcgtg gagatcgcga tgcaggccgc gctcgccggc gacgaccgcc gcgtgcgcca
     8521 gatgcactgg ggcggcggca cgcccacgtt cctttccgat cacgagctgc gcgtggtctg
     8581 ggaactgctg cgctccaact tcgagttctc gcccgatggc gagtacgcga tcgagatcga
     8641 cccgcgctcg tgctcgccgg acacgatccg cctcctcgcg gagctgggct tcaaccgcgc
     8701 gagcttcggg gtgcaggatt tcgacccggc ggtgcagcgc gcggtcaatc gcatccagcc
     8761 ctacgagatg acgcgcgacg tgatcgacgc ctcgcgcgcg agcggcttca agtcgctcaa
     8821 cgtggacctg atctacggcc tgcccaagca gagcgtgtcg agctttgccg acaccgtgga
     8881 gcgcgtgctg gaactgcgcc ccgaccgcat cgccctctac agctacgccc acctgccgca
     8941 gcgcttcaag ccgcagcgtc gcatcctcga cgtcgacctc cccagcgccg cggaaaagct
     9001 ttccatcatg gcgcgggcca tcgacctgtt caaccgcgcg ggctacatct acatcggcat
     9061 ggaccacttc gcgctgcccg acgacgaact cgcgcgcgcg cagcgctgcg gcacgctcac
     9121 gcgcaatttc cagggctata ccgtcgggcc ggacggcgac atgctttcgt tcggcatctc
     9181 ggccatcggc aaggtggggc ccacgtacag ccagaacgcg cgcgacctgc ccgcctacta
     9241 cgacacgctg gaacaggggc ggcttcccgt catccgcggc atcgagcttt ccgccgacga
     9301 cctgttgcgc cgcgccatca tcgtcgcgct catgtgccag ttctgcgtct cgatcgaatc
     9361 catcgaggcg gcgcacctgg tgaagttcga cgactatttc gccttcgaga tcgaggcgct
     9421 caaggaattc gaggaactcg gcctcgtgga acgcgagccg ggctggattg ccgtcacccc
     9481 caagggccgc ttcttcgtgc gcgccatctg cgccgtcttc gaccgctacc tgcgcgccga
     9541 caaggcgcgc gtcgcctact cgcgcatcat ctgaagggcc gcgggaaagg tccgacgtga
     9601 ccggcgctct cgcactcagc gcgatgctcc tggcggtcta tggcgcagcg cattgcgccg
     9661 ccatgtgcgg cggcttcgtc gcctccacgt tcacccccgc ctcaggcagc ccggcccgtc
     9721 cggccgtgtt gcaccgggcc gcgctgccca tgcatgccgg acgcctcctt gcctacgcag
     9781 cggccggcgc cctcgcgggc gcgctgggcg cagctcccgc ggtgttcctg gggtcggcgc
     9841 gctaccaggc cgtcctgttt tccgtgggag cgctggtcct cgtcgtcacg ggcctgcgca
     9901 tcgcggggat tcgcctcgtt cgcccggcga atgccctctt agcgcaacct ctctggaact
     9961 gggcggcgaa cgaggcgcgc cgccttggcg ccccggacac gctcccgaag agactgggcc
    10021 tgggcgtcct gtggggctgc gcaccctgcg ccatcgtgta ctcggcgctt ccgttggcgc
    10081 tcgcgagtgg ttcggcaacg ggcggagcgc tggtgatgct ggccttcggc gccggaacgc
    10141 taccggcact ggtcgcggcc ggatggctca tcggccgcgg agaagcgctg ttccagggca
    10201 ggaccgcgcg gcgcgtcgcg ggcctgctga tcgtcgtgct ggccctggcc ggcatcgcgc
    10261 aggcgctggg cgtggccgat acggcactgg ggtcattctg cgtctcggcg ccgcactgac
    10321 tattcccggc cgccgttgcg cccctcgttg actctcacct tgacgattcc cgcaacgatt
    10381 ccgcgctcgt cgatcatcac ggaaagatcc gtggcatatc cccaggcatc ctggtagcgg
    10441 taatcc
//
LOCUS       DASUEV010000151         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1630210, whole genome shotgun sequence.
ACCESSION   DASUEV010000151 DASUEV010000000
VERSION     DASUEV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630210"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFV55_04950"
     CDS             complement(<1..204)
                     /locus_tag="VFV55_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4943678.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTLVLSKKYGGEV
                     RAYDQIDNAPEEKARGITINTAHV"
     gene            complement(223..2328)
                     /gene="fusA"
                     /locus_tag="VFV55_04955"
     CDS             complement(223..2328)
                     /gene="fusA"
                     /locus_tag="VFV55_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX4943679.1"
                     /translation="MARKTPIDRYRNIGISAHIDAGKTTTTERILFYTGVSHKMGEVH
                     DGAAVMDWMEQERERGITITSAATTCFWKGMDGNLPEHRVNIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYRVPRLAFVNKMDRTGANFYKVYDQMKE
                     RLKANPVPVQIPIGAEDKFEGVVDLVRMKAIYWDEASQGMKFDLREVPAELLDKANEW
                     REKMVESAAEANEELMNKYLETGSLTEAEIKFGLRTRTIASEIVPMMCGSAFKNKGVQ
                     AMLDGVIDYMPAPTDIPPVKGELENGQPTERRAADDEPFAGLAFKIMTDPFVGQLIFF
                     RVYSGVINSGDTIYNPIKFKKERIGRILQMHANQREEIKEVRAGDIAAAVGLREATTG
                     DTICNPDKVIILERMIFPEPVISQAVEPRTKADQEKMGIALNRLAQEDPSFRVHTDEE
                     SGQTIIAGMGELHLEILVDRMRREFGVEASVGKPQVAYRETIRKTVTDVEGKFIKQSG
                     GRGQYGHVVLKVEPQEAGKGFEFVDAIKGGVVPREFIPAVEKGLRETLPNGVLAGYPV
                     VDVKVTLHYGSYHEVDSNENAFKMAASIGFKEGMRRASPVLLEPMMAVEVETPEDYAG
                     TVMGDLSSRRGMVQGMDDIPGGGKVIKAEVPLSEMFGYSTSLRSATQGRATYTMEFKH
                     YSEAPKNVAEAIINQKAAA"
     gene            complement(2394..2864)
                     /gene="rpsG"
                     /locus_tag="VFV55_04960"
     CDS             complement(2394..2864)
                     /gene="rpsG"
                     /locus_tag="VFV55_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX4943680.1"
                     /translation="MPRRREVPKREILPDPKFKSEEVAKFINVLMTRGKKSVAERIMY
                     GALDQIAKKSGKDPFEVFGAAIGNVKPMVEVKSRRVGGANYQVPVEVRPIRRTALAMR
                     WLREAARKRGEKSMGQRLANELLEAAEGRGGAVKKREEVHRMAEANKAFAHYRF"
     gene            complement(2893..>3142)
                     /gene="rpsL"
                     /locus_tag="VFV55_04965"
     CDS             complement(2893..>3142)
                     /gene="rpsL"
                     /locus_tag="VFV55_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX4943681.1"
                     /translation="KPNSALRKVAKVRLTNGYEVISYIGGEGHNLQEHSVVLIRGGRV
                     KDLPGVRYHMVRGSLDTAGVKDRKQSRSKYGAKRPKAA"
BASE COUNT          415 a         1073 c          998 g          656 t
ORIGIN      
        1 cacgtgcgcc gtgttgatcg tgatcccgcg cgccttctcc tccggcgcgt tgtcgatctg
       61 gtcgtacgcc ctcacctcgc caccgtactt cttcgacagc accagcgtca tcgccgccgt
      121 cagcgtcgtc ttcccgtggt ccacgtggcc gatcgtcccc acgttcacgt gcggcttcgt
      181 ccgctcaaac ttgccctttg ccatatcggt tcctcgtgaa tatcaggctg cagctttttg
      241 gttgatgatc gcttcggcga cgttcttcgg cgcctcggag tagtgcttga attccatcgt
      301 gtaggttgcg cggccctggg tcgccgagcg caggctcgtc gagtagccga acatctccga
      361 caggggaacc tcggccttga tcaccttccc gccccccggg atgtcgtcca tgccctgcac
      421 catgccccgg cgcgacgaca ggtcgcccat gaccgtgccg gcgtagtcct cgggcgtctc
      481 gacctcgacc gccatcatcg gctcgaggag caccgggctc gcccggcgca tgccttcctt
      541 gaagccgatc gaggcggcca tcttgaacgc gttctcgttc gagtcgacct cgtggtacga
      601 gccgtagtgc agcgtcacct tgacgtccac gaccgggtag ccggccagca cgccgttggg
      661 gagcgtctcg cgcaggccct tctcgaccgc cgggatgaac tcgcgcggca ccacgccgcc
      721 cttgatggca tcgacgaact cgaagccctt gcccgcctcc tgcggctcga ccttgagcac
      781 gacgtggccg tactggccgc ggccgcccga ctgcttgatg aacttgccct cgacgtcggt
      841 caccgtcttg cggatggtct cgcggtaggc gacctggggc ttgcccacgc tcgcctcgac
      901 gccgaactcg cggcgcatgc ggtcgaccag gatctccagg tgcagctcgc ccatgccggc
      961 gatgatcgtc tgcccgcttt cctcgtcggt gtggacgcgg aacgacgggt cttcctgggc
     1021 caggcgattg agggcgatgc ccatcttctc ctggtccgcc ttggtcctgg gctccacggc
     1081 ctgggagatc accggctcgg ggaagatcat gcgctcgagg atgatcacct tgtccgggtt
     1141 gcagatggtg tcgccggtcg tggcctcgcg caggcccacg gccgcggcga tgtcgcccgc
     1201 gcgcacttcc ttgatctcct cgcgctggtt ggcgtgcatc tgcaggatcc ggccgatgcg
     1261 ctccttcttg aacttgatcg ggttgtagat cgtgtcgccc gagttgatca cgcccgaata
     1321 gacgcggaag aagatgagct ggccgacgaa cgggtcggtc atgatcttga acgcgaggcc
     1381 cgcgaaaggc tcgtcgtcgg ccgcgcggcg ctcggtcggc tggccgttct ccagttcgcc
     1441 cttgaccggc gggatgtcgg tcggcgccgg catgtagtcg atcacgccgt cgagcatggc
     1501 ctgcacgccc ttgttcttga acgccgagcc gcacatcatc ggcacgatct cgctcgcgat
     1561 cgtgcgcgtg cgcaggccga acttgatttc ggcctccgtc aggctgcccg tctcgaggta
     1621 cttgttcatg agctcttcgt tcgcctcggc ggcgctctcc accatcttct cgcgccattc
     1681 gttggccttg tcgaggagct cggccggcac ctcgcgaagg tcgaacttca tgccctggga
     1741 cgcctcgtcc cagtagatcg ccttcatcct cacgaggtcg accacgccct cgaacttgtc
     1801 ctcggcgccg atgggaatct ggaccgggac ggggttcgcc ttgaggcgct ccttcatctg
     1861 gtcgtagacc ttgtagaagt tggcgccggt gcggtccatc ttgttcacga acgcgaggcg
     1921 cggcacgcgg tacttgttgg cctggcgcca caccgtctcg gactgcggct gcacgccgcc
     1981 gaccgcgcaa tagaccatgc acgccccgtc caggacgcgc atcgagcgct cgacctcgat
     2041 ggtgaagtcg acgtggcccg gcgtgtcgat gatgttgacg cggtgctcgg gaaggttgcc
     2101 gtccatgccc ttccagaagc aggtcgtcgc ggccgacgtg atcgtgatgc cgcgctcgcg
     2161 ctcctgctcc atccagtcca tgacggccgc accgtcgtgg acctcgccca tcttgtgcga
     2221 tacacccgtg tagaaaagga tgcgctccgt ggtcgtggtc ttgccggcgt cgatgtgcgc
     2281 cgagataccg atgttgcggt agcggtcgat gggtgtcttg cgggccacgg tgtgctctct
     2341 gtcgtctcga tgccgccgag cctcttgtgg cggctcgcgg cggggttctt cgtctagaag
     2401 cggtagtggg cgaatgcctt gttggcctcg gccatgcggt gcacttcctc gcgcttcttc
     2461 acggcgccgc cgcggccttc cgcggcctcg aggagctcgt tggccaggcg ctggcccatg
     2521 ctcttctcgc cgcgcttgcg ggcggcctcg cgcagccagc gcatggcgag cgcggtgcgg
     2581 cggatcgggc ggacctcgac cggcacctgg tagttggcgc cgccgacgcg ccggctcttg
     2641 acctccacca tcggcttcac gttgccgatg gccgcgccga acacctcgaa gggatccttg
     2701 ccggacttct tcgcgatctg gtcgagcgcg ccgtacatga tccgctcggc aacgctcttc
     2761 ttgccgcgcg tcatcagcac gttgatgaac ttggcgactt cctcgctctt gaacttggga
     2821 tccggcagga tctcgcgctt cgggacttct ctgcggcggg gcataggtct ctatccgttc
     2881 agtaagggtg aatcaagcgg ccttgggccg tttggcgccg tacttcgagc gcgactgctt
     2941 ccggtccttg acgcccgcgg tatcgaggct gccgcgaacc atgtggtagc gaacgcccgg
     3001 caggtccttc acgcggccgc cgcggatgag caccaccgag tgctcctgga ggttgtgccc
     3061 ctcgccgccg atgtagctga tgacctcgta gccgttggtg agacgcacct tggcgacctt
     3121 ccgcagcgcc gagttcggct tc
//
LOCUS       DASUEV010000152        15233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1630797, whole genome shotgun sequence.
ACCESSION   DASUEV010000152 DASUEV010000000
VERSION     DASUEV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 15233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15233
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630797"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="arsS"
                     /locus_tag="VFV55_04970"
     CDS             <1..789
                     /gene="arsS"
                     /locus_tag="VFV55_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164889.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX4943682.1"
                     /translation="VLAFVAASGATTLDLTGGAPELNPHFRPLVARARAMGVKVIDRC
                     NLTILEEPGHEDLAGFLAALRVDIVASLPCYTQELVDRQRGKGVYEKSVRAIGRLNEL
                     GYGREGSGLVLNLVYNPQGPTLPPAQEKLEADYKRILGETFGLSFNALFTLANMPIQR
                     FGSTLISKGQFNAYMQLLHSAHRDENVDGVMCRSLVSVDWQGYVYDCDFNQMLGLPLR
                     VAGKARPHLTDLVGRDLDGNPIVVRDHCYGCTAGQGSSCGGALA"
     gene            810..1766
                     /locus_tag="VFV55_04975"
     CDS             810..1766
                     /locus_tag="VFV55_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943683.1"
                     /translation="MPDMSPEARPGRSCPTAYRYPPRVFDRRPDLEAGTLFVVGGLYG
                     NVEALEEIAAMAARETQPVTIVFNGDFHWFDVAAGDFERVTEGVLRHAAIRGNVETEI
                     AGEDSRAGCGCAYPADVSDADVARSNEILGRLRETARGFPALRQQLGRLPMHLVARVG
                     AARVGIVHGDCASLAGWGFAQDALDDPVRVRWIENGFRDARVDVFASSHTCLPVTRDF
                     EFAHGRAVVANNGAAGMPNFAGERFGLVTRISVHPARDALYGTRVAGAFVDTLAVRYD
                     VEAWQARFLASWPEGSPAHQSYFRRIAQGPGFALARALPIAA"
     gene            1791..2840
                     /locus_tag="VFV55_04980"
     CDS             1791..2840
                     /locus_tag="VFV55_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4943684.1"
                     /translation="MNRPAAATAGTKAVSLALQGGGSHGAYAWGILDRFLEDGRLSFE
                     GLSGTSAGSVNAALCAYGLLLGGAEGAREKLDAFWRGIARTGSFFNPGGRFPFEAAVP
                     GWPTQRSLTVEAFKAMTGSLSPYQWNPLNFNPLRDALENHVDFERLRTCKVTKLFVSA
                     TNVRTGRGRVFKTDEVSVDVVLASACLPQLFQAVEIEGDPYWDGGFMGNPVLYPLFYE
                     TRSRDVIIVHINPIERPGTPRLPHEIENRLNEITFNASLIKELRAVAFVQKLLAEGWI
                     KDEFRGQLKDVLMHSIRADKALCDLSVASKFDVSWPFLAELRNRGRETAGEWLEMNWK
                     DIGLRSTVDVRKEFL"
     gene            2857..5079
                     /locus_tag="VFV55_04985"
     CDS             2857..5079
                     /locus_tag="VFV55_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4943685.1"
                     /translation="MKGGKIVVAIALAALVAAYFAFDLGRFLSLDYFKAQQEAIAAYF
                     RANPLQSAAIYFAAYVAVTGLSLPGAAIMTLAGGAIFGLLWGVVLVSFASTIGATLAF
                     LASRFLLRDWVQAKLGDKLKPINDGIAKEGAFYLFALRLVPAFPFFVVNLVMGLTPMR
                     TWTYAWVSQAGMLAGTVAYVYAGTQLAGITSIRGILSPGLVGAFVLLGIFPFIAKRIV
                     AALKARKVYAKWKRPARYERNVVVIGAGSAGLVASYIAAAVKAKVTLVERHRMGGDCL
                     NTGCVPSKALIRSAKFLSHVRRSKEFGIRGATADFDFAEVMERVQAVIEAVEPHDSVE
                     RYSGLGVECLAGTARITDPWTVEIALEDGGTRVLTTKSIVIAAGARPFVPPIPGLAQV
                     APLTSDNVWNLRALPKRLVVLGGGPIGSELTQAFARFGSKVTQVEMLPRIMIREDPEV
                     SEMVKKSFEADGVDVLVNHKAKEVVVEGGEKYLVVESAGAEKRIAFDEILVAVGRVAN
                     TADYGLEELGIPVTKQRTVEVNEFLETAYPNIYACGDVAGPYQFTHTAAHMAWYASVN
                     ALFGRLRRFKVDYSVIPWATFTEPEVARVGLSEQEAKEKGIAHQVVTYGIDDLDRAIA
                     DSEAHGFVKVIVKPGSDRILGATIVGEHAGDLIAEFVAAMRHGIGLDKVLGTIHIYPT
                     LAEANKYAAGAWKRSTVTHGQMDFLAAFQDWTRGAGGLGAVLGRVPSLLRDKRPYWGK
                     "
     gene            5082..5900
                     /locus_tag="VFV55_04990"
     CDS             5082..5900
                     /locus_tag="VFV55_04990"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX4943686.1"
                     /translation="MTRFVLALALAVFSASSFAFDHTHAAWDALVKKHVKVVDGGKAS
                     QVRYADFARDRPALQAYLADVSKVSEAEFRGWTKPQQMAFLINAYNGFTVELILTKYP
                     DLKSIRDLGTFLSSPWTKKFFKLFGAETSLDAIEHEMLRKPGAYDEPRVHYAVNCASI
                     GCPMLREEAYVAPRLDAQFEEQARRFLGDHTRNRFNPASGKLEVSEIFKWFKEDWTSG
                     YKGIGSAGPVNSREQYFARYADLLADGPGQRKLVAEGRAPITHLDYDWSLNDAR"
     gene            5916..6611
                     /locus_tag="VFV55_04995"
     CDS             5916..6611
                     /locus_tag="VFV55_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX4943687.1"
                     /translation="MSRLSIIVPALDEAAGIRECLAALQPMRERGTEVLVVDGGSADA
                     TARLAAPQADRVITAPRGRAAQMNAGAAIAAGDTLLFLHADTRLPEGADGLVLAGLAA
                     SGRAWGRFDVAIEGGSRLLAVVAAFMGARSRLTAIATGDQAIFVRREAFRRAGGFPSI
                     ALMEDIAFSRAMKRQGPPLCLRERAVTSARRWERHGTVRTILLMWWLRLAYFLGADPA
                     RLAQRYGHGRAAR"
     gene            6592..7230
                     /locus_tag="VFV55_05000"
     CDS             6592..7230
                     /locus_tag="VFV55_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX4943688.1"
                     /translation="MAARRDSAVQVAVFAKAPVPGEVKTRLVGMLGPEGAAQLHATLA
                     RHALATAREAGVGPVSLWCAPDAGHPFFADCAAQYGVLLHDQRGAHLGERMARAFGQL
                     LATGPALLLGSDCPSLDAGDLRAAAGSLATHDAVIQPAEDGGYVLVGLAREMSSIFEG
                     IRWGGPDVMRDTRARLREASAIWREMPARWDVDRPEDYRRLLAAGFFAETGR"
     gene            7227..7961
                     /locus_tag="VFV55_05005"
     CDS             7227..7961
                     /locus_tag="VFV55_05005"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX4943689.1"
                     /translation="MKAHVAAAVFAAAVVACPRALASGADIAPFSQARPGQALPEGWR
                     PLNLARIAPPEVALVEDGGVTVLRFRAVAAAGTVAHDLDSLPAQRPLLSWRWKVDRVV
                     ERGNLAEKSGDDFAARVYVFYDLPVSSLPFGARVKVLLARAIWGEKLPTAAICYVWDN
                     RHAPGTSVWNPYTDRVRTVVLRSGSPGAWVEEARDLEADFRAAFGSQWAGPAPRVTGI
                     AIGNDTDQTGEAVTAWFGDFRLGARP"
     gene            7958..9091
                     /locus_tag="VFV55_05010"
     CDS             7958..9091
                     /locus_tag="VFV55_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4943690.1"
                     /translation="MKRLVLLGGGHAHVHVLKAIGDALDPAVSVTLVTPVERQVYSGM
                     VPGFVAGHYGLAECGIDLGPLATRARALVVRSSAALVNPAMREVVCANGEIVPYDVLS
                     IDVGSRPFVGGARGVAEHAIAIRPLESFVDGWERVLARARGGGMGSVSVVGGGAAGIE
                     LALAMDYRFRKEAGEGAPHVRILTDARMLLPEHTAAVRGRLLRIARSHNVGLHVQSLV
                     AEVGPGFLRLKDNIEFATDATFWVTGATAHEFIRDSGFVTDERGFLGVNEFMQSVSHP
                     EVFGAGDCATDLRNPRAKAGVFAVRAGPALVANLFAALHGGPLERQVPARRFLALISC
                     GGHYAVGVYGGFSFEGGWVWRWKDHIDRRFIARYAPEALASNG"
     gene            complement(9259..9813)
                     /locus_tag="VFV55_05015"
     CDS             complement(9259..9813)
                     /locus_tag="VFV55_05015"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEX4943691.1"
                     /translation="MVRFIERERLPLATSPAHIAERILVLRGHKVMIDADLAELYGVE
                     TKRLNEQVRRNQARFPRDFMFQLTLAERSEVVANCDHLRKLKFSHSLPYAFTEHGALM
                     AASILNTERAVEVSVYVVRAFVQLREMLVSNKDLAAQLAQLERKFATHDQAIASIIAT
                     IRQLMAPPQAQKKPPIGFVRPKEK"
     gene            complement(9913..11223)
                     /locus_tag="VFV55_05020"
     CDS             complement(9913..11223)
                     /locus_tag="VFV55_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEX4943692.1"
                     /translation="MEEIPLSWLVVALATLLLSSGFFSIAETSMMALNRYRLKHLVRE
                     GRRSARLTQDLLAHTDRLLGVILLGNNLLNSASTVVATLICVRLFGEGEVTLAIATAA
                     VTFFILVFSEITPKVIGARFPEAIALPSAFVLTPLLRIAYPIVWFVNLFVQALLKLLF
                     IRPRADSESQLSMAELRTLVLEGGKFIPAKHQSIFLNLFELEDMTVDDTMTPRGQIEA
                     IDLEDDIDDIRTAISTAHHTRLVVFDGSLDKVAGILHVRKFLNASRREPLDREGIREI
                     LRDPYYVPEGTPLLTQLTNFQDQMRHVGLVVDEYGELLGLVTLQDILEEIVGEFTNQK
                     PLLGSLLRKEPDGSIIAEGTCPLRVLNRKAGFHFPLDGPKTVNGLVLEALEDIPEPGT
                     ALIIAGHPMEILQVQNRMVKVVRIRARKAGGDEARAALPSQASR"
     gene            complement(11272..12105)
                     /gene="ccsA"
                     /locus_tag="VFV55_05025"
     CDS             complement(11272..12105)
                     /gene="ccsA"
                     /locus_tag="VFV55_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX4943693.1"
                     /translation="MRDSALYFSTAACWLALAALAWRGAHAVVAAGPSPGGGAFRLEG
                     VLLPVALVLHGMLVYSGVFVDEGLNLGVSKSVSLIIWLTVAIYWLASIAVPGLASIQG
                     LWAPVALAAVVLQFLLPSHYLVHYGGDPLFTLHFVIAMLAYSLFIVATMHALVMLAEQ
                     KWLHRGVMPPFLKSLPPLLEMEALLFRILFAAFVLLTLTVVSGVFFSEQLFRRPFQFT
                     HKTVFGIISWIIFGSLLWGRYFRGWRGKRAVYWTLWGFGALLLAYLGSKFVLEIILHR
                     G"
     gene            12152..13501
                     /gene="ffh"
                     /locus_tag="VFV55_05030"
     CDS             12152..13501
                     /gene="ffh"
                     /locus_tag="VFV55_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX4943694.1"
                     /translation="MLDALTQRLSSVIKTMRGHARITEENVTEALREVRMALLEADVG
                     LPVVKDFIARVKEKALGQEVLGSLTPGQALVGVVHGELVTLMGGTNAQLNLATQPPAV
                     ILLAGLQGAGKTTSAGKLARLLKGAKKKVLLVSVDIYRPAAIEQLKSLAAQLSIDLFP
                     SDASQKPVEIAANALDWARKHYHDVLIVDTAGRLAIDEAMMAEVKAIHAAVHPIETLF
                     VVDSMQGQDAVNVARAFGEALPLTGVILTKLDGDARGGAALSVRTITGAPIKYAGVSE
                     KMDGLEPFHPERMASRILGMGDIVSLVEEARKSVDEGEAKKLADKFRKGKDFDLEDFK
                     SQIQQMKKMGGVSALMDKLPAQLTRAAGQSTDLQEKSIRRTEGIIDAMTPLERRKPDL
                     IKASRKRRIAAGAGVQVQEVNRLLNQFEQMQKMMKMMRSGGLAKMMRNMKGMLPGMR"
     gene            13537..14646
                     /locus_tag="VFV55_05035"
     CDS             13537..14646
                     /locus_tag="VFV55_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943695.1"
                     /translation="MKPLAHLLAAVAAACSITAFGADKIVVGQSAPLTGSNAEIGKDI
                     RDGALAWFARVNASGGINGKRVELVSLDDKNDRKTAGANAVKLVNESGAVALFGFASA
                     TLSLDAMPIVKEKKVPFFAPFTGADAIHKQNHFVFVMRASYADELVKILDHWKSLGVT
                     RVTVINYDDEIGNQNYATVARLMEGAGKKAVGVKLKRNAAVEKAQIDAIIASDPQVIV
                     ATTLYGATSQVIKGLRAAGKPYNMTSLSFVGPSQLAKAAGADAAGVSVAGVVPPPTKT
                     AVPVIKECGEAIRKAGIAELNYTNLEACIAAKVLTEAMRRSGREVTRESLYRSLNGLG
                     SLDVGGYVVNFSPENRHGNRYVELAVIGKNGQFRF"
     gene            14898..15173
                     /gene="rpsP"
                     /locus_tag="VFV55_05040"
     CDS             14898..15173
                     /gene="rpsP"
                     /locus_tag="VFV55_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX4943696.1"
                     /translation="MVVIRMARGGAKNRPYYTIVVADSRMPRDGRFIERVGFYNPKAA
                     GNEPGFRISLDRVAHWVSQGAQPSDAVKKLIKRGKVQLAGKPEEPAK"
BASE COUNT         2450 a         4993 c         5345 g         2445 t
ORIGIN      
        1 gtcctggcct tcgtcgcggc gagcggggcc acgacgctcg acctgaccgg cggcgccccg
       61 gagctcaatc cccatttccg gcccctcgtg gcgcgcgcaa gggcgatggg ggtgaaggtg
      121 atcgaccggt gcaacctcac catcctggag gagccgggcc atgaggacct cgccgggttc
      181 cttgccgcgc ttcgcgtgga tatcgtcgcc tcgctcccgt gctacacgca ggagctggtg
      241 gaccggcagc gcggcaaggg cgtgtacgag aagagcgtgc gcgccatcgg ccgcctgaac
      301 gagctgggct acgggcgcga aggctcgggg ctggtgctga atctcgtcta caacccgcag
      361 gggccaaccc ttcccccggc gcaggagaag ctggaggcgg actacaagcg gatcctcggc
      421 gagaccttcg ggctttcgtt caacgcactc ttcacgctgg ccaacatgcc catccagcgc
      481 ttcggctcga cgctcatctc gaaggggcag ttcaacgcct acatgcagct gctgcactcg
      541 gcgcaccgcg acgagaatgt cgacggcgtg atgtgccgct ccctcgtgtc ggtggactgg
      601 cagggctacg tgtacgactg cgacttcaac cagatgctgg gactgccgct gcgcgtggcg
      661 ggcaaggccc gtccccacct taccgacctc gtggggcggg acctcgacgg caatccgatc
      721 gtcgttcgcg accactgcta cggctgcacc gccgggcagg gctcgtcctg cggcggggcg
      781 ctcgcctgac gtcgcggagg aacaagccga tgcctgacat gtctcccgaa gcccgcccgg
      841 ggcgaagctg cccgaccgcc taccgctatc cgccgcgcgt attcgaccgc cgccccgacc
      901 tggaagccgg gacgctcttt gtcgtgggcg ggctctacgg caacgtcgag gcgctcgagg
      961 agatcgccgc catggccgcg cgcgaaacgc agcccgtgac gatcgtcttc aacggcgact
     1021 tccactggtt cgacgtggcg gccggcgact tcgagcgggt gacggaaggg gtgctgcgcc
     1081 acgcggcgat ccgcggcaat gtggaaaccg agatcgccgg cgaggattcg cgggcgggtt
     1141 gcggctgcgc ctatcccgcg gacgtgagcg atgccgacgt ggcgcgctcc aacgagattc
     1201 tcgggcggct gcgcgaaacc gcgcggggct tcccggcact gcgccaacag ctggggcgcc
     1261 tgccgatgca cctcgtggcg cgcgtgggcg cggcgcgggt gggcatcgtg cacggcgatt
     1321 gcgcctcgct cgcgggctgg ggcttcgcgc aggacgccct cgacgacccc gtccgtgtgc
     1381 gctggatcga gaacggattc cgcgatgccc gcgtcgatgt gttcgcgagc agccacacgt
     1441 gcctgccggt cacgcgcgac ttcgagttcg cgcacggccg cgcggtggtg gccaacaatg
     1501 gagcggcagg catgcccaat ttcgcgggcg agcgcttcgg gctcgtcacg cgcatttccg
     1561 tgcaccccgc cagggacgcc ctctacggca cgcgggtagc aggcgccttc gtcgacacgc
     1621 tcgccgtccg gtacgacgtg gaggcgtggc aggcacgctt tctcgcaagc tggcccgagg
     1681 gctctcccgc ccaccaatcc tatttccgcc gcatcgcgca gggccccggc ttcgcgctcg
     1741 cgcgggcgct gccgatcgcc gcctgaacca tggcgcgcgc gcggcggccc gtgaatcgcc
     1801 ccgcagcggc gacggcgggc accaaggcgg taagcctcgc gctccagggc ggcggctcgc
     1861 acggcgccta tgcctggggc atcctcgacc gtttcctcga ggacggccgg ctttccttcg
     1921 aggggctctc cggcaccagc gccggttcgg ttaatgcggc gttgtgcgcc tacgggctcc
     1981 tgctcggcgg tgcggaaggc gcgcgcgaga agctggacgc cttctggcgc ggcatcgcca
     2041 ggacgggctc gttcttcaat ccgggtggca ggttcccctt cgaggcggcc gtgccgggct
     2101 ggcccacgca gcgctcgctc accgtggagg cgttcaaggc gatgaccggc tcgctttcgc
     2161 cctaccagtg gaacccgctc aacttcaatc ccctgcgcga cgcgctcgag aaccacgtcg
     2221 atttcgaacg gctgcgcacc tgcaaggtga cgaagctttt cgtctcggcc accaacgtgc
     2281 gcacgggcag ggggcgcgta ttcaagaccg acgaggtgag cgtcgacgtg gtcctcgcct
     2341 cggcctgcct gccgcagctc ttccaggccg tcgagatcga gggcgatccg tactgggacg
     2401 gcggcttcat gggcaatccg gtgctctatc cgctcttcta cgagacgcgt tcgcgcgacg
     2461 tgatcatcgt ccacatcaat cccatcgagc ggccgggcac cccgcggctg ccgcacgaga
     2521 tcgagaaccg gctgaacgag atcaccttca acgcctccct catcaaggaa ctgcgcgccg
     2581 tggccttcgt gcagaagctg ctcgcggagg gctggatcaa ggacgaattc cgcgggcagt
     2641 tgaaggatgt cctcatgcac tccatccgcg ccgacaaggc attgtgcgat ctctcggtgg
     2701 cgagcaagtt cgacgtgagc tggccattcc tcgccgagct gcgcaaccgc gggcgggaga
     2761 ccgccggtga atggctggag atgaactgga aggatatcgg gctgcgctcc acggtcgacg
     2821 tgcgcaagga gttcctgtga gcgaagggag catttcgtga aaggcggaaa gatcgtcgtc
     2881 gcgatcgccc ttgctgccct cgtggcggcc tatttcgcct tcgatctcgg gcgcttcctg
     2941 tcgctcgact acttcaaggc gcagcaggag gcgatagccg cctacttccg ggccaacccg
     3001 ctgcaatcgg ccgcgatcta cttcgccgcc tacgtcgcgg tgaccgggct gtcgcttccc
     3061 ggcgcggcca tcatgacgct cgcggggggc gcgatcttcg gcctgctgtg gggcgtggtg
     3121 ctcgtctcct tcgcctccac catcggcgcc acgctggcct tcctcgcatc gcgcttcctg
     3181 ctgcgcgact gggtgcaggc gaagctcggc gacaagctga agccgatcaa cgacggcatc
     3241 gcgaaggaag gggcgttcta cctgttcgcg ttgcggctcg tgccggcgtt cccgttcttc
     3301 gtggtgaacc tggtgatggg gctcaccccc atgcgaacct ggacctacgc ctgggtgagc
     3361 caggcgggca tgctcgcggg gacggtcgcc tacgtctatg cgggaacgca gctcgcaggg
     3421 atcacttcga tcaggggcat cctctcgccg ggcctcgtgg gcgcattcgt gctgctcggc
     3481 atcttcccgt tcatcgccaa gcgcatcgtc gctgccctga aggcacgcaa ggtgtacgcc
     3541 aagtggaagc gcccggcccg gtacgagcga aacgtggtgg tgatcggcgc gggctccgcc
     3601 gggctcgtgg cgtcctacat cgccgcggcg gtgaaggcaa aggtgacgct cgtcgagcgc
     3661 caccgcatgg gcggggactg cctcaacacc ggctgcgtgc cctccaaggc ccttatccgg
     3721 tcggcgaagt tcctgtcgca cgtgcgccgg tcgaaggagt tcggcatccg cggcgccacg
     3781 gcggatttcg atttcgccga ggtgatggag cgtgtgcagg cggtgataga ggcggtggag
     3841 ccgcacgatt cggtggagcg ctactccggc ctgggcgtcg aatgcctcgc cggcacggcg
     3901 aggatcaccg acccctggac ggtggagatc gcgctggaag atggcggcac gcgggtgctc
     3961 acgacgaaaa gcatcgtcat cgcggcgggc gcgcggccgt tcgtgccccc catcccgggg
     4021 ctcgcccagg tggcgccgct cacctccgac aacgtgtgga acctgcgcgc gctgccgaag
     4081 cggctggtgg tgctcggcgg cgggcccatc ggctcggaac tcacccaggc cttcgcgcgc
     4141 ttcgggtcga aggtcacgca ggtggagatg ctgccgcgga tcatgatccg cgaggacccc
     4201 gaggtttcgg agatggtgaa gaagagcttc gaggccgacg gcgtcgatgt cctcgtgaat
     4261 cacaaggcga aggaggtcgt ggtcgagggc ggggagaaat acctcgtggt ggaaagcgct
     4321 ggcgcggaaa agcgcatcgc attcgacgag atccttgtcg ccgtggggcg cgtcgccaac
     4381 accgcggact acggactgga ggaactgggc atcccggtca cgaagcagcg cacggtggaa
     4441 gtgaacgagt tcctcgagac ggcctacccc aacatctacg cctgcggcga cgtggccggg
     4501 ccgtaccagt tcacgcacac ggcggcgcac atggcgtggt atgcgtccgt caatgcgctg
     4561 ttcggaaggc tgcgccgttt caaggtggac tattcggtga tcccgtgggc caccttcacc
     4621 gagcccgagg tggcccgcgt ggggctgagc gagcaggaag cgaaggaaaa gggcatcgcg
     4681 caccaggtgg tcacctacgg catcgacgac ctcgaccgcg ccatcgccga cagcgaggcg
     4741 cacggcttcg tcaaggtgat cgtgaagccc ggaagcgacc ggatcctggg cgccacgatc
     4801 gtgggcgagc acgcgggcga cctgatcgcc gagttcgtcg ccgccatgcg ccacggcatc
     4861 ggcctggaca aggtgctggg caccatccac atctacccca cgctcgccga ggccaacaag
     4921 tacgcggccg gcgcgtggaa gcgttcgacc gtcacgcatg ggcagatgga tttcctcgcc
     4981 gccttccagg actggacgcg cggcgcgggt ggcctcggcg ccgtcctcgg ccgcgttccc
     5041 tcgctgctgc gcgacaagcg tccttattgg ggaaagtgat catgacccgg ttcgttctcg
     5101 cattggcgct cgcggtcttc tccgcgtcgt cgttcgcctt cgatcacacc cacgcggcgt
     5161 gggacgccct ggtgaagaag cacgtcaagg tggtcgacgg cggcaaggcc tcgcaggtgc
     5221 gctacgcgga tttcgcccgg gaccgccccg cgctgcaggc ttacctcgcg gacgtgtcga
     5281 aagtgagcga ggccgaattc cgcggctgga cgaaaccgca gcagatggcg ttcctcatca
     5341 acgcctacaa cggtttcacg gtggagctca tcctcacgaa atacccggac ctgaagtcga
     5401 tccgcgacct cggcaccttt ctcagcagcc cctggacgaa gaagttcttc aagctgttcg
     5461 gggccgaaac ctccctcgac gcaatcgagc acgagatgtt gaggaagccc ggggcgtacg
     5521 acgagccgcg cgtgcattac gccgtgaact gcgcctccat cggctgcccg atgcttcgcg
     5581 aggaggcgta cgtggccccc cgcctcgacg cccagttcga ggaacaggca cggcgcttcc
     5641 tcggcgacca cacgcgcaat cgcttcaatc cggccagcgg caagctggag gtctcggaga
     5701 tcttcaagtg gttcaaggag gactggactt ccggctacaa gggaatcggc agcgccggcc
     5761 cggtgaactc gcgcgagcag tacttcgccc gctatgccga cctgctggcc gacgggcccg
     5821 ggcagcgcaa gctcgtcgcc gagggccggg cgccgatcac ccatctcgat tacgactggt
     5881 ccctcaacga tgcccgttga cgcgataatc gcgtcgtgag ccgcctgtcg atcatcgtgc
     5941 cggcgctgga cgaggccgcg ggaatccgcg agtgcctggc cgcgctgcaa ccgatgcgcg
     6001 agcgcggcac ggaagtcctc gttgtcgatg gcgggagcgc ggacgccacg gcaaggctcg
     6061 ccgcgccgca ggccgaccgg gtcatcacgg cgccgcgtgg ccgcgccgcg cagatgaacg
     6121 ccggggccgc gattgccgcc ggcgacacgc ttctcttcct gcacgcggac actcgccttc
     6181 ccgaaggcgc ggacgggctc gtgctcgcgg gacttgccgc ctcggggcgc gcctggggcc
     6241 gcttcgacgt ggcgatcgag ggtggatcgc ggctgctggc cgtcgtggcc gcgttcatgg
     6301 gcgcgcgatc gcgccttacc gccatcgcca ccggcgacca ggcgatattc gtccggcgcg
     6361 aggcgttccg gcgcgcgggc ggctttccgt cgatcgcgct catggaggac atcgccttct
     6421 cgcgcgccat gaagcgccag ggcccgccac tgtgcctgcg cgaaagggcg gtgacgtccg
     6481 cgcgccgctg ggagcggcac ggcacggtgc gcacgatcct gctcatgtgg tggctgcgtc
     6541 tcgcctattt cctcggtgcc gatcccgcgc gcctcgcgca acgctacggc catggccgcg
     6601 cggcgcgata gcgcggtgca ggtcgccgtg tttgccaagg cgccggttcc cggcgaggtg
     6661 aagacacggc tcgtcgggat gctcggcccg gaaggtgcgg cgcaactgca tgcaacgctg
     6721 gcgcgccatg cgctggcaac ggcccgcgaa gccggtgtcg gccccgtctc cctgtggtgc
     6781 gcgccggacg ccgggcaccc gttcttcgcc gactgcgccg cgcaatacgg cgtactgctg
     6841 cacgaccagc gcggtgccca cctcggggag cgaatggccc gcgcattcgg ccagctcctc
     6901 gccacgggcc cggcgttgct cttgggctcg gattgtccct cgctcgatgc cggcgacctg
     6961 cgcgccgcgg ccggttcgct ggccacgcac gatgcggtga tccagcccgc ggaggacggc
     7021 ggttatgtcc tcgttggcct ggctcgcgaa atgagctcga ttttcgaggg catccgctgg
     7081 ggcgggccgg acgtgatgcg cgatacccgc gcccgcctgc gcgaagcttc cgccatctgg
     7141 cgcgagatgc cggcccgctg ggatgtggac cggcccgagg attaccggcg ccttctcgcc
     7201 gcggggtttt tcgcggagac cggccggtga aggcccacgt tgccgcagcg gtcttcgcgg
     7261 cagccgtcgt ggcatgcccg cgcgcgctgg cttcgggcgc ggacatcgcg cccttctcgc
     7321 aggcaagacc gggccaggcg ctgccggaag ggtggcgccc gctcaacctt gcacgcatcg
     7381 ccccgcccga ggtggcgctg gtcgaggatg ggggcgtgac ggtgctgcga ttccgggcag
     7441 ttgccgccgc cgggaccgtg gcgcacgacc tcgactcctt gcccgcgcaa cggcccttgc
     7501 tgtcctggcg ctggaaggtg gaccgcgtgg tcgagcgcgg caacctcgcg gagaagagcg
     7561 gcgacgattt cgcggctcgc gtgtacgtct tctacgacct gccggtgtct tcccttccgt
     7621 tcggcgcgcg cgtgaaggtg ctgctcgccc gcgcaatctg gggcgagaag ctgcctaccg
     7681 ccgcgatctg ctatgtctgg gacaatcgcc atgcgcccgg gacgagcgtg tggaatccct
     7741 ataccgaccg cgtccggacc gtggtgctgc gcagcggctc gcccggcgca tgggtggagg
     7801 aagcgcgcga cctcgaggcc gacttccgcg ccgcgttcgg ctcgcagtgg gcggggcccg
     7861 cgccgcgcgt cacgggcatc gccatcggca acgacaccga ccagacgggc gaggcggtca
     7921 cggcgtggtt cggggacttc cggctggggg cgcggccatg aagcgactcg tgctcctggg
     7981 aggggggcac gcgcacgtgc acgtgctgaa ggcgatcggc gacgcgctcg atcccgccgt
     8041 cagcgtgacc ctcgtgacgc cggtcgagcg gcaggtgtac tcgggcatgg tgccgggatt
     8101 cgtggccggg cattacgggc tggccgaatg cggcatcgac ctgggcccgc tcgcgacgcg
     8161 cgcccgtgcg ctcgtggtgc gatcttccgc cgccctcgtg aacccggcga tgcgcgaggt
     8221 ggtttgcgcc aacggcgaga tcgtgcccta cgacgtgctc tcgatcgacg tgggttcgcg
     8281 gccgttcgtc ggcggcgcca ggggcgttgc ggagcacgcc atcgccatac ggcccctgga
     8341 gagtttcgtc gacggatggg agcgcgtgct ggcccgtgcg aggggcggcg gcatgggctc
     8401 ggtgtcggtc gtgggcggcg gcgcggccgg catcgagctg gcgctcgcga tggattaccg
     8461 cttccggaag gaggccgggg agggcgcgcc gcacgtgcgc atcctcaccg acgcgcgcat
     8521 gctgctgccc gagcacaccg ctgccgttcg cgggcggctc ctgcgcatcg cgcgctcgca
     8581 taatgtcggc ctgcacgtgc agagcctcgt ggccgaggtg ggcccgggat tcctgcggct
     8641 gaaggacaac atcgagttcg ccaccgacgc aacgttctgg gtcacgggcg cgacggccca
     8701 cgaattcatc cgcgactcgg gattcgtcac ggacgagcgc ggcttcctcg gggtcaacga
     8761 attcatgcag tcggtctccc atcccgaggt gttcggcgcg ggcgactgcg cgaccgacct
     8821 gcgcaacccg cgcgccaagg cgggcgtgtt cgcggtgcgc gcgggcccgg cgctggtggc
     8881 caacctgttc gccgccctgc atggcggccc gctggagcgg caggtgccgg cgcgccgctt
     8941 cctcgcgctc atctcgtgcg gcgggcacta cgccgtgggc gtctacggcg gattctcctt
     9001 cgagggcggc tgggtgtggc gctggaagga ccacatcgac cggcgcttca tcgcgaggta
     9061 tgcgccggaa gcgctcgcct caaacggctg aatctgtaca tcgcgatgcg gtgaaccgcg
     9121 tgttatcggc aactgttaca ggcggcgcgt acatccggcg gcatcaggtt cgtgatccag
     9181 aattcgatca tggtggcctc ttcttgttgg cgttcttgcg ttgcgctggc ttcttgtgac
     9241 ctcaaagcgc gaggagcgct acttctcttt gggcctcacg aatccgatcg gcggtttctt
     9301 ctgcgcctgc ggcggggcca tcaactggcg gatggtggcg atgatgctgg cgatggcttg
     9361 gtcgtgggtg gcgaacttgc gttcgagttg ggcgagctgg gcggcgagat ccttgttgga
     9421 gacgagcatc tcgcgcagtt gcacgaaggc gcgcacgacg tagacgctca cttcgacggc
     9481 acgctcggtg ttgaggatgg aggcggccat gagcgctccg tgctcggtga aggcgtaggg
     9541 cagcgagtgg gaaaacttga gcttccggag gtggtcgcaa tttgcgacca cctcggagcg
     9601 ctcggcgagc gtgagctgga acatgaagtc gcgcggaaag cgcgcttggt tgcgacgcac
     9661 ctgctcgttg aggcgcttgg tttcgacgcc gtagagttcg gccaggtccg cgtcgatcat
     9721 gaccttgtgg ccgcggagaa cgaggatgcg ttcggcgatg tgcgcggggc tcgtggccaa
     9781 tggcaagcgc tcccgttcga tgaacctgac cataagctat tcccggcttc cggaaacgcg
     9841 attcaagatg ccatgccagg aaaacgcgcc tattgcttgc cagtttccgg tggatgctgg
     9901 ttacgatttc ccttagcggc tagcctggga ggggagggcg gcccgtgcct cgtccccgcc
     9961 cgccttgcgc gcgcggatgc gcaccacctt caccatgcgg ttctgcacct ggaggatctc
    10021 catcggatgc ccggcgatga tgagcgccgt gccgggctcc gggatgtcct cgagggcctc
    10081 gaggaccagg ccgttgaccg tcttgggacc gtcgagcggg aagtgaaagc cggccttgcg
    10141 gttgaggacg cgaagcgggc aggtgccctc ggcgatgatc gagccgtcgg gttccttgcg
    10201 cagcaggctt cccagcagcg gcttctggtt ggtgaactcg ccgacgatct cctcgaggat
    10261 gtcctgcagc gtgaccagtc cgaggagctc gccgtactcg tcgaccacca ggcccacgtg
    10321 gcgcatctgg tcctggaagt tggtgagctg cgtgagcagc ggcgtgccct ccgggacgta
    10381 gtagggatcg cgcaggatct cgcggatgcc ctccctgtcg agcggttcgc ggcgcgaggc
    10441 gttgaggaac ttgcgcacgt gcaggatgcc ggccaccttg tccaggctgc cgtcgaagac
    10501 cacgaggcgc gtgtggtgcg cggtggaaat ggcggtgcgg atgtcgtcga tgtcgtcctc
    10561 gaggtcgatg gcctcgatct gcccgcgcgg cgtcatcgtg tcgtcgaccg tcatgtcctc
    10621 cagctcgaag aggttgagga agatcgactg gtgcttggcc gggatgaact tgccgccctc
    10681 gaggacgagc gtgcgcagtt ccgccatcga gagctggctc tcggaatcgg cgcgcggccg
    10741 gatgaagaga agcttgagca gcgcctgcac gaagaggttc acgaaccaca cgatcgggta
    10801 ggcgatgcgc agcagcggcg tgaggacgaa ggcggaggga agcgcgatcg cctcggggaa
    10861 gcgcgcgccg atcaccttcg gcgtgatctc gctgaagacg aggatgaaga acgtgacggc
    10921 ggcagtggcg atggcgagcg tcacctcgcc ctcgccgaag aggcgcacgc agatgagggt
    10981 ggcgaccacc gtggaggcgg agttgaggag gttgttgccg agcaggatca cgcccagcag
    11041 gcggtcggtg tgggcgagga gatcctgggt gaggcgcgcc gagcgcctgc cctcgcgcac
    11101 gaggtgcttc agccggtagc ggttgagcgc catcatgctc gtctcggcga tcgagaagaa
    11161 gcccgacgac aggagcagcg tcgccaacgc gacaacgagc cagcttagcg ggatttcttc
    11221 catcgggatg ggggcgtttg ttgtcccgcc cggcggcggg agcgcgggcg ctcagccccg
    11281 gtgcaggatg atctcgagca cgaacttgct gccgaggtac gcgagcagca gcgccccgaa
    11341 accccaaagc gtccagtaca ccgcgcgctt gccgcgccag ccgcggaagt accggcccca
    11401 gagcagcgac ccgaagatga tccacgagat gatgccgaag accgtcttgt gcgtgaactg
    11461 gaacgggcgc cggaagagct gctcggagaa gaacacgccc gagacgacgg tcagcgtgag
    11521 cagcacgaac gcagcgaaga ggatgcggaa gaggagcgcc tccatctcga gcagcggcgg
    11581 caggctcttc aggaacggcg gcatcacgcc ccggtgcagc cacttctgct cggcaagcat
    11641 caccagcgcg tgcatggtcg ccacgatgaa caggctgtag gcgagcatcg cgatcacgaa
    11701 gtgcagggtg aagagcgggt cgccgccgta gtgcacgagg tagtgcgacg gcagcaggaa
    11761 ctgcagtacc accgccgcga gcgcgaccgg cgcccacagg ccctggatgc tcgccaggcc
    11821 cggcacggcg atgctcgcga gccagtagat ggcgaccgtg agccagatga tgagggagac
    11881 ggacttggag acgccgaggt tcaggccctc gtccacgaaa acgccggagt agaccagcat
    11941 gccatgaagg acgagcgcca ccggcaggag gacaccttcg agccggaatg cgcctccgcc
    12001 gggcgagggg cccgcggcca cgacggcgtg cgcgccccgc caggcgagtg ccgcgagggc
    12061 cagccagcag gcggcggtgc taaaatagag ggccgaatcg cgcattttcg aagcgattga
    12121 agtctacacc aattcccctt cggtccttcc catgctggat gccctgacgc agcgactttc
    12181 ctcggtcatc aagacgatgc gcggccacgc gcgcatcacc gaggagaacg tgacggaggc
    12241 cctgcgcgag gtgcgcatgg cgctgctgga ggccgacgtg ggcctgcccg tggtgaagga
    12301 cttcatcgcg cgggtgaagg agaaggcgct cgggcaggaa gtgctaggca gcctcacgcc
    12361 cggccaggcg ctggtgggcg tggtgcacgg cgagctggtg accctcatgg gcggcaccaa
    12421 cgcgcagctc aacctggcga cgcaaccccc ggcggtgatc ctcctcgcgg gcctgcaggg
    12481 cgcgggcaag accacgagcg cgggcaagct cgcgcggctg ctcaagggcg cgaagaagaa
    12541 ggtgctcctg gtctccgtcg acatctaccg tcccgcggcc atcgagcagc tgaagtcgct
    12601 cgccgcgcag ctttcgatcg acctctttcc gtccgatgct tcgcagaaac cggtcgagat
    12661 cgccgcgaat gccctcgact gggcgaggaa gcactaccac gacgtgctca tcgtggacac
    12721 ggcggggcgc ctggcgatcg acgaggcgat gatggccgag gtgaaggcca tccacgcggc
    12781 ggtccacccg atcgagaccc tcttcgtggt cgactccatg cagggccagg acgcggtcaa
    12841 cgtcgcccgg gctttcggcg aggcgctgcc gctcaccggc gtcatcctca ccaagctcga
    12901 cggcgacgcg cgcgggggcg cggcgctctc ggtgcgcacg atcaccggcg cgcccatcaa
    12961 gtacgccggc gtgtcggaaa agatggacgg cctcgagccc ttccacccgg agcgcatggc
    13021 ctcgcgcatc ctcggcatgg gcgacatcgt ctcgctcgtg gaggaggcgc ggaaatcggt
    13081 ggacgagggc gaggcgaaga agctcgccga caagttcagg aagggcaagg acttcgacct
    13141 cgaagacttc aagtcgcaga ttcagcagat gaagaagatg ggcggggtat cggcactcat
    13201 ggacaagctc ccggcgcagc tcacccgggc ggccggccag tccacggacc tgcaggagaa
    13261 gtcgatccgc cgcaccgagg gcatcatcga cgcgatgacg ccgctcgagc ggcgcaagcc
    13321 cgacctcatc aaggcctccc gcaagcggcg catcgcggcg ggggcagggg tgcaggtgca
    13381 ggaggtgaac cggctcctca accagttcga gcagatgcag aagatgatga agatgatgcg
    13441 ctcgggcggg ctggcgaaga tgatgcgcaa catgaagggc atgctgcccg gcatgcgttg
    13501 agcggcgacg gtcgtacccc ctatttcgga gaattcatga aaccccttgc gcacctgctg
    13561 gcggcggttg ccgccgcctg ttccatcacg gccttcggtg ccgacaagat cgtcgtcggg
    13621 cagtccgcgc ccctcaccgg ctccaacgcc gagatcggca aggacatacg cgacggtgcc
    13681 ctggcctggt tcgcgcgcgt caacgcatcc ggcggcatca acggaaagcg ggtggagctg
    13741 gtctccctcg acgacaagaa cgaccgcaag acggccggcg ccaacgcggt gaagctggtg
    13801 aacgagtccg gcgccgtcgc gcttttcggc ttcgcctccg cgacgctctc gctcgacgcc
    13861 atgccgatcg tgaaggagaa gaaggtgccc ttcttcgccc cgttcaccgg cgccgacgcc
    13921 atccacaagc agaaccattt cgtcttcgtg atgcgcgcct cctacgccga cgagctggtg
    13981 aagatcctcg atcactggaa gtcgctgggc gtcacgcggg tcaccgtcat caactacgac
    14041 gacgagatcg gcaaccagaa ctacgcgacc gtcgcgcgcc tcatggaagg cgcgggcaag
    14101 aaggcggtgg gcgtgaagct caagcgcaac gccgccgtgg agaaggcgca gatcgacgcc
    14161 atcatcgcga gcgacccgca ggtgatcgtc gcgacgacgc tctacggcgc cacctcccag
    14221 gtcatcaagg ggctcagggc cgcgggcaag ccctacaaca tgacctcgct ttcgttcgtc
    14281 ggccccagcc agctcgccaa ggccgcgggc gccgatgcgg cgggcgtatc ggtggcgggc
    14341 gtcgtgccgc cgccgaccaa gacggcggtg ccggtgatca aggagtgcgg cgaggccatt
    14401 cgcaaggcgg gaatcgccga gctcaactac accaacctcg aggcgtgcat cgcggccaag
    14461 gtgcttacgg aggccatgcg caggtcgggc cgggaggtca cccgcgagag cctctaccgc
    14521 tcgctcaacg ggctgggctc cctcgatgtc ggcggctacg tggtcaattt ctcgccggag
    14581 aaccgccacg gcaaccgcta cgtggagctc gcggtcatcg gcaagaacgg gcagttccgc
    14641 ttctagggtc aaaggggtca ggttacttta ctgtcgaagg tcaaaagggt caggttactt
    14701 tacacatcac ccggcagatg ccgggaatca tcgacggtaa agtaacctga cccctttaac
    14761 ccgaccgtcg cttgcacgga aagggccaag cccttataat gcaaggcttt tccgtttccg
    14821 ggttgcaggt tcccggggac gaatccggca gctttctcga gcaactgtta cccgttatca
    14881 acgggatgga gccataaatg gtcgtcatca gaatggcgcg cggcggcgcg aagaaccgcc
    14941 cctactacac catcgtcgtc gccgactcgc gcatgccgcg cgacgggcgt ttcatcgagc
    15001 gcgtcggctt ctacaacccc aaggcggccg gcaacgaacc gggtttccgg atatcgctcg
    15061 accgcgtggc ccactgggta tcccagggcg cgcagccgtc ggatgcggtg aagaagttga
    15121 tcaagcgtgg caaggtccag ctcgccggca agccggaaga gcccgccaag tagcgcgccg
    15181 ggttccccgt acacggggga cgaggcgcgg ctggtggtga tggggcgcat cct
//
LOCUS       DASUEV010000153        10623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_373112, whole genome shotgun sequence.
ACCESSION   DASUEV010000153 DASUEV010000000
VERSION     DASUEV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373112"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFV55_05045"
     CDS             complement(<1..726)
                     /locus_tag="VFV55_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX4943697.1"
                     /translation="MKFLDLARATRASGTLRLPGSKSISNRTLLLAALAEGTTQVRGL
                     LDSDDTRVMLESLRRLGIALARGERDGDWQVTGAPGGFAVKEAALFLGNAGTAFRSLT
                     AALALADGHYRLEGVPRMHERPIGDLVDALRQAGARIDYLAKEGFPPLAIAPRDRRAG
                     MRVQVRGDVSSQFLTAILIALGATGGGVRVEVAGELISKPYVEMTLSLMRRFGVEVAR
                     EGWEAFVVPPGARYVSPGSIDVEG"
     gene            complement(729..1616)
                     /locus_tag="VFV55_05050"
     CDS             complement(729..1616)
                     /locus_tag="VFV55_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX4943698.1"
                     /translation="MRRLKTIAVIGVGLIGGSFALAMRRAGAVTKIVGVDRDAQALER
                     AAALGVIDTAAESASEAATGADLVFVAVPVRGIGPVLHDVALGLGPDAVVTDAGSTKA
                     DVARTAAAELRDRMPRFVPGHPIAGREASGVDAAVADLFRGARVVLTPEPETSPEAAA
                     LVRACWEACGARVTEMSALAHDRIFASVSHLPHMLAFALVSEIVSRPDAAELLGFAAG
                     GFRDFTRIAASSPEMWRDIALANREALVEELDRYAARVAVFRDLVEKGDGPGLQRLMT
                     EARNARRAWTGGRRSSSDE"
     gene            complement(1624..2727)
                     /gene="hisC"
                     /locus_tag="VFV55_05055"
     CDS             complement(1624..2727)
                     /gene="hisC"
                     /locus_tag="VFV55_05055"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX4943699.1"
                     /translation="MSPRDLAPAHVREIAPYVPGKPIAETARELGMAESAILKLASNE
                     NPLGSSPRAIEALRGALGDLAWYPDGSGHDLKSAICAKLAVVPGNIVLGNGSNDVLEL
                     VARAFLTPADTAVYSRHAFMVYPLVVQAIGARGIEVPAKDYGHDLAAMAAAIRPDTKL
                     VFLANPNNPTGTFVPWAEVRAFVERVPRTTLVVIDEAYGEYLPAERQSPTVGWIAGFP
                     NLVVTRTLSKAYGLAGLRVGFGLAHPEVAELMNRVRQPFNVNHLAMVAACAALDDEAF
                     IAQSRRVNAAGLVQLAKGFERLGLEFIPSSGNFISVRVGNAARIYDSLLRQGVIVRPI
                     AGYGMPEHLRVTVGTEPQNERFLASLERALEGA"
     gene            complement(2732..3802)
                     /gene="pheA"
                     /locus_tag="VFV55_05060"
     CDS             complement(2732..3802)
                     /gene="pheA"
                     /locus_tag="VFV55_05060"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX4943700.1"
                     /translation="MTAELDRLRGEIDRIDGEILERLNQRAQFARAIGTLKVGQAYRP
                     EREAQVLRRIKENNPGPLGDETVAYIFREIMSACLALERPITVAYLGPQGTFSESAAM
                     KHFGLAAVTMPMPSIDEVYRAVESGAADFGVVPVENSTEGAVGRSLDLMPQTPAKACG
                     EVLIRIHHHLLSRDAAASPSAIKRIYSHSQSLAQCHEWLNNNAPNAERMAVASNAEAA
                     RRAFEEPGTAAVAGDRAAEYYKLAILAANIEDEPNNTTRFLIIGDYEPKRSGRDRTSL
                     VLSARNRAGAVYEMLTPFATRGVSMTRFESRPSKVALWEYLFFVDIEGHRDDANVAAA
                     LEEVGRIAGYLKVLGSYPVAVL"
     gene            complement(3799..4992)
                     /locus_tag="VFV55_05065"
     CDS             complement(3799..4992)
                     /locus_tag="VFV55_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX4943701.1"
                     /translation="MAAPFRIATWNAISANGLRRFPAHAYRVEHALAEPDAILLRSHV
                     LDAASVPASVKAIARSGAGVNNVPVAEMSRRGVPVFNAPGANANAVKELVMAAIFLAA
                     RNIVPAVDGVRALAPGADYERRVEEGKKVFAGVEVRGKTLGVVGLGAIGGLVADAALK
                     LGLRVVGYDPQVTAETVSRLPAPVRLVASVDECFAAADFVTLHVPLLESTRHLADARR
                     LALGPPGAVLLNFSRAAIVDEEAVVAAVASGRLKSYVCDFPSPRFAGQAGIVALPHLG
                     ASTEEAEEASAAMVIDQLRDFFEEGTVRNAVNFPAVEMPRESPHRVAIANANVPNMLG
                     MISTTMARSSLNIHNMVNKSKGEMAYTLVDLDSEVRAGVLEELSHIPGVLSVRLVSPL
                     PEPRA"
     gene            complement(5000..6079)
                     /gene="serC"
                     /locus_tag="VFV55_05070"
     CDS             complement(5000..6079)
                     /gene="serC"
                     /locus_tag="VFV55_05070"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX4943702.1"
                     /translation="MHVWNFGSGPAMLVPAVLEQAKAELLDWHGRGMSVMEMSHRGKD
                     FVAIAAQAEADLRALLAIPANYKVLFLQGGATAQFAAVPMNLLRGKSEADFVVTGEWS
                     KKAFSEARKYCRPNLAATAEDRKFTYVPRQASWKLSPGAAYVHVCTNETIGGVEYQWT
                     PDTGDVPLVSDMSSHILSRPVDVSRFGLIYAGAQKNIGPAGLVIVIVREDLIGQALPG
                     TPNILDFKLQADADSMLNTPPTFSVYLAALTFKWLLAKGGLAVMERENLAKASLLYDC
                     IDANPFYSNPVAKEDRSRMNVPFRLKDDALDARFLAEAEALGLSQLKGHRSVGGMRAS
                     IYNAMPLEGVAALVEFLGDFARRNG"
     gene            complement(6128..8746)
                     /gene="gyrA"
                     /locus_tag="VFV55_05075"
     CDS             complement(6128..8746)
                     /gene="gyrA"
                     /locus_tag="VFV55_05075"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4943703.1"
                     /translation="MEQFAKETHPISIEQEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLYAMHELSNDWNRAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYPL
                     IDGQGNFGSVDGDNAAAMRYTEIRMAKVAHELLADLEKETVDFGPNYDGSESEPLILP
                     ARFPNLLVNGSSGIAVGMATNIPPHNLNEVVDACIAVLGNPDIDIEELIAIIPAPDFP
                     TAGIIYGTAEVKEGYRTGRGRVVMRARAHIEDMEKANKQAIVVDELPYQVGGDQLLKR
                     VSELVHEKKIEGIADLRNESDKGGMRLVIELKRGEVAEIVLNNLYKLTQMQETFGMNM
                     VALLDGQPKLLNLKQFLDAFLRHRREVVTRRTVFDLRKARERGHVLEGLAVALSNVDE
                     VIAIIKAAATPPLAKVELMSRTWRSQLVEDMLSRATSDLFRPENLPKDYGLAGNGYRL
                     SDVQAQRILEMQLQRLTGLEQDKILSEYREVIAAIEDLLDILAKPERVTRIISDELLE
                     IKREFGDKRRSEIVVNGQDLSMEDLIAPEDVVVTLSHAGYVKSQKLDEYGAQKRGGRG
                     KQATTTKEDDFIEKLFIANTHDYVLCFSNKGKVYWIKVYEVPLGSRASRGKPIVNLLK
                     MDQDERINAILPVREFVEDRYVFFATQEGVVKKTALSDFSRPRASGIIAVDLDEGDFL
                     IGVALTDGKHDVMLFSDEGKAVRFDENDVRPMGRGARGVRGMNLAKGGKVIALLVAEN
                     EEQSVLTATENGYGKRTSIAEYTRHGRGTQGMIAIHTSARNGKVVAATLVSNDDEIML
                     ITSGGVLIRTRVKEVRVMGRSTQGVTLINLGEGEKLSGLQTVMDRDVEENGNGNGNGN
                     GNGNGNGHDVPDAPAA"
     gene            9056..9700
                     /locus_tag="VFV55_05080"
     CDS             9056..9700
                     /locus_tag="VFV55_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4943704.1"
                     /translation="MKISSFGKLLLGSVAAAAFASGSALAQSPQPILTDSQGVPVRDA
                     SGNCVLTSGIIHPDCGEKKEAPAPKPAEPAAPAAPAAPAAPTPPAPKPAPASVKQTIV
                     IQADALFDFDKSVVRPDGRKNIDAALAKLGGLDLEMIIATGHTDSVGTDAYNQKLSER
                     RAAAVKAYLVSKGIPASKITTLGKGESQPVATNKTKEGRQKNRRVDIEFKGVKK"
     gene            9760..>10623
                     /locus_tag="VFV55_05085"
     CDS             9760..>10623
                     /locus_tag="VFV55_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4943705.1"
                     /translation="MREVDTLIHAAWIAPVEPAGVLLERHCVAVRDGRIEALLPSDDA
                     PGTFTARTEVRLDRHLLIPGLVNLHGHAAMALLRGIADDLPLMTWLKDHVWPAEAKHV
                     GDEFVHDGSLLAAAEMLRGGITCFNDMYFFPEATARAALKAGIRASLGMIAVEFPSAY
                     AADATGYLHKGLATRDAYRGEELLSFCLAPHAPYTLADETLSRIATLAEEIDAPIHTH
                     VHETADEIAQGIAQHGMRPLERLRRLGLVGPRLIAVHAVHLEEAEIDLLAHEGASIAH
                     CPSSNLKLASGI"
BASE COUNT         1627 a         3643 c         3646 g         1707 t
ORIGIN      
        1 gccctcgacg tcgatcgagc ccggcgacac gtagcgggcg cccggtggca cgacgaatgc
       61 ctcccatccc tcccgcgcga cctcgacgcc gaagcggcgc atcagggaga gcgtcatctc
      121 gacgtagggt ttcgagatga gctctcccgc cacctccacg cgaacgccgc cacccgtcgc
      181 gcccagtgcg atgaggatcg cggtgaggaa ctggctcgac acgtcgccgc gcacctgcac
      241 gcgcatgccc gcgcgccggt cgcgcggcgc gatggcgagc ggcgggaagc cctccttcgc
      301 gaggtaatcg atgcgggcac cggcctggcg cagggcatcg accaggtcgc cgatgggccg
      361 ctcgtgcatg cgcggcaccc cttcgagccg gtagtgcccg tcggccagcg cgagcgcagc
      421 cgtgagcgag cggaacgccg tgcccgcgtt gcccaggaag agcgcggcct ccttcaccgc
      481 aaaccctccg ggcgcgccgg tcacctgcca atcgccgtcg cgctcgccgc gcgccagtgc
      541 aatgccgagg cggcgaaggg attcgagcat cacgcgggtg tcgtcagaat cgaggagccc
      601 gcgaacctgc gtggtccctt cggccagcgc ggcgagaagg agcgtgcggt tggagatgct
      661 cttcgacccg ggaaggcgca gcgtgcccga tgcgcgggtg gcgcgcgcga ggtcgaggaa
      721 tttcatgttc actcgtcgga ggagctgcgc ctgccgcccg tccaggcgcg ccgggcgttg
      781 cgcgcctcgg tcatcagccg ctgcaggccg ggcccgtcgc ccttttccac gagatcgcgg
      841 aacacggcaa cgcgggccgc gtagcggtca agctcctcga ccagcgcctc gcggttggcg
      901 agcgcgatgt cgcgccacat ctccggcgaa ctggccgcga tgcgggtgaa gtcgcggaag
      961 ccgcccgccg cgaagccgag cagctccgcc gcgtcgggcc gcgagacgat ctcggagacg
     1021 agggcgaagg cgagcatgtg cggcaagtgg cttaccgagg cgaagatgcg gtcgtgtgcc
     1081 aaggcggaca tctccgtcac gcgcgcgccg cacgcctccc agcaggcgcg cacgagcgct
     1141 gcggcctccg gggaagtctc gggttccgga gtgagcacca cgcgtgcgcc gcggaaaagg
     1201 tcggccaccg ccgcatccac cccgctcgcc tcgcgcccgg cgatcggatg gccggggacg
     1261 aatcgcggca tccggtcgcg cagttccgcg gcggccgtgc gcgcgacgtc agccttggtg
     1321 ctgcccgcgt ccgtgaccac cgcgtcgggg ccaagcccca gcgccacgtc atgcaggacc
     1381 ggtccgatgc ctcgcaccgg gaccgccaca aagaccaggt cggcgccggt ggccgcctcc
     1441 gatgccgatt cggcagccgt gtcgatgacc ccgagcgccg cggcgcgttc cagggcctgg
     1501 gcgtcgcgat cgacgccgac gatcttcgtg accgcgcccg cccggcgcat cgcgagcgcg
     1561 aacgagcctc cgatgaggcc cacgccgatg accgcgatcg tcttcaggcg ccgcaacgca
     1621 acctcaggcg ccttcgagcg cgcgttcgag cgaggcgagg aagcgctcgt tctgcggctc
     1681 cgtcccgacc gtcacgcgaa ggtgttccgg catcccgtag cccgcgatcg gccgcacgat
     1741 cacgccttgc cggaggagcg agtcgtagat gcgcgccgca ttgcccacgc gcacggagat
     1801 gaagttgccc gacgagggaa tgaactcgag gcccaggcgc tcgaagccct tcgcgagctg
     1861 cacgaggccc gccgcattga cgcgccggct ctgcgcgatg aacgcctcgt cgtcgagggc
     1921 cgcgcaggcc gcgaccatgg cgaggtgatt cacgttgaac ggctgccgga cccggttcat
     1981 gagctcggcg acctccgggt gcgccagccc gaaacccacg cgcagccccg cgaggccgta
     2041 ggccttggaa agcgtgcgcg tcacgacgag gttcgggaag cccgcgatcc agcccaccgt
     2101 gggcgactgg cgctccgcag gcaggtattc gccgtaggcc tcgtcgatca ccacgagggt
     2161 cgtgcgcggg acgcgctcca cgaaggcgcg cacttccgcc cacggcacga acgtgcccgt
     2221 cggattgttc ggattcgcga ggaagacgag tttcgtgtcc ggacggatcg cggccgccat
     2281 cgcagccagg tcgtggccgt agtccttcgc gggcacttcg atgccgcgcg cgccgatcgc
     2341 ctggaccacg agcgggtaca ccatgaaggc gtggcgcgaa tacacggcgg tatccgccgg
     2401 cgtgagaaag gcgcgcgcga cgagctcgag cacgtcgttc gagccgttgc cgagaacgat
     2461 gttcccgggg accacggcga gcttcgcgca gatcgccgac tttaggtcgt ggccgctgcc
     2521 gtcgggatac caggccaggt cgcccagcgc gccgcgcagg gcctcgatcg ccctgggcga
     2581 cgagcccagc gggttttcgt tggacgcgag cttgaggatc gccgactcgg ccatacccag
     2641 ctcgcgcgcc gtttcggcga tgggcttgcc gggcacatag ggggcgattt cccggacgtg
     2701 cgccggggca aggtctcttg ggctcatgga ttcaaaggac ggccacggga taggagccga
     2761 gcaccttgag gtagccggcg atgcggccca cctcctcgag cgcggcggcg acgttggcgt
     2821 cgtcccggtg gccttcgatg tcgacgaaga agaggtattc ccagagcgcg accttcgacg
     2881 ggcgcgactc gaaccgggtc atcgagacgc cgcgcgtggc gaagggcgtg agcatctcgt
     2941 agacggcgcc ggcgcggttg cgcgccgaaa ggacgagcga ggtgcggtcg cggccggaac
     3001 gcttcggctc gtagtcgccg atgatgagga agcgcgtcgt gttgttgggt tcgtcctcga
     3061 tgttcgcggc gaggatcgcg agcttgtagt actccgccgc gcggtcgccg gcgacggccg
     3121 ccgttccggg ctcttcgaag gcgcggcggg ccgcttcagc gttgctcgcg accgccatgc
     3181 gctcggcatt gggcgcattg ttgttgagcc actcgtggca ctgcgccagc gactggctgt
     3241 gcgagtagat gcgcttgatc gcggaggggc tcgctgccgc gtcgcgcgaa agcaggtggt
     3301 ggtggatgcg gatgagcacc tcgccgcagg ctttggcggg cgtctgcggc atgaggtcga
     3361 gcgaacgccc cacggcgccc tcggtggagt tttccacggg caccacaccg aagtcggccg
     3421 cgcccgattc caccgcgcgg tagacctcgt cgatcgacgg catgggcatc gtgacggcgg
     3481 ccagcccgaa gtgcttcatg gccgcgcttt cgctgaacgt gccctgcggc ccgaggtagg
     3541 ccacggtgat ggggcgctcg agggcgaggc aggccgacat gatctcgcgg aagatgtagg
     3601 cgaccgtctc gtcgccgagc ggcccgggat tgttctcctt gatccgccgc aggacctgcg
     3661 cctcgcgctc gggccggtag gcctgcccca ccttgagcgt gccgatggcg cgcgcgaact
     3721 gcgcgcgctg gttgagccgc tcgaggatct cgccgtcgat gcggtcgatc tcgccgcgca
     3781 ggcgatcgag ctcggccgtc acgcacgcgg ctccggcaac ggggacacca ggcgcaccga
     3841 gagcacgccg gggatgtgcg agagttcctc cagcacgccc gccctgacct cgctgtcgag
     3901 gtcgacgagg gtgtaggcca tctccccttt cgacttgttg accatgttgt ggatgttgag
     3961 gctgctgcgc gccatcgtag tcgaaatcat cccgagcatg ttgggcacgt tcgcgttcgc
     4021 gatggccacg cggtgcgggg attcgcgcgg catctcgacc gccgggaagt tgacggcgtt
     4081 gcgcaccgtg ccctcctcga agaagtcgcg cagctggtcg atcaccatcg cggcactcgc
     4141 ctcctccgcc tcctccgtcg aggccccgag gtgcgggagc gcgacgatgc ccgcctgccc
     4201 cgcgaagcgc gggctgggga agtcgcagac gtaggacttg agcctgcccg acgcgaccgc
     4261 ggcgaccacc gcctcctcgt ccacgatggc ggcgcgcgag aagttgagga gcaccgcgcc
     4321 gggaggtccg agcgcgaggc gcctcgcatc cgcgaggtgg cgcgtggatt ccagcagggg
     4381 cacgtgcaag gtcacgaaat ccgcggcggc gaagcattcg tcgaccgagg cgacgaggcg
     4441 caccggcgcc ggcagccggg acaccgtttc ggccgtgacc tgcgggtcgt agccgacgac
     4501 gcgaagcccg agcttgagcg cggcgtcggc gaccagcccg ccgatggcac cgagccccac
     4561 gacgccgaga gtcttgccgc gcacctcgac tcccgcgaac accttcttgc cctcttccac
     4621 ccggcgctcg taatccgccc ccggcgcgag ggcgcgcaca ccgtcgaccg cgggcacgat
     4681 gttgcgcgcg gcgagaaaga tggcggccat gacgagttcc ttcacggcat tggcgttggc
     4741 gccgggcgca ttgaagaccg gcacgccgcg ccggctcatt tcggccaccg gaacgttgtt
     4801 cacgcccgcg ccggagcgcg cgatcgcctt cacgctggcc gggaccgaag cggcgtccag
     4861 cacgtgggag cgaagcagga tcgcgtcggg ctcggcgagc gcgtgctcca cgcggtaggc
     4921 gtgcgcggga aagcggcgca gtccgttcgc ggagatcgcg ttccaggtgg cgatgcgaaa
     4981 cggcgcggcc atcgggcagc tacccgttgc ggcgcgcgaa gtccccgagg aactcgacga
     5041 gcgccgccac gccttccagg ggcatggcgt tgtagatcga ggcgcgcatg ccgcccaccg
     5101 agcggtggcc cttgagctgc gagagcccga gcgcctccgc ctcggcgagg aagcgcgcgt
     5161 ccagcgcgtc gtccttcagg cggaagggga cgttcatgcg cgagcggtct tccttcgcaa
     5221 ccgggttcga atagaagggg ttcgcgtcga tgcagtcgta gaggagcgag gccttggcca
     5281 ggttctcgcg ctccatgacc gcaagaccgc ctttggccag gagccacttg aaggtgaggg
     5341 ccgcgaggta gacggagaag gtcggcggcg tgttcagcat cgagtccgcg tccgcctgca
     5401 gcttgaagtc gaggatgttc ggcgtgccgg gaagtgcctg cccgatgagg tcctcgcgca
     5461 cgatcacgat gaccagcccg gccggcccga tgttcttctg cgcgcccgcg tagatgaggc
     5521 cgaagcgcga gacgtcgacg ggccgcgaca ggatgtgcga ggacatgtcg gagacgagcg
     5581 gcacgtcgcc ggtatcgggc gtccactggt actcgacacc gccgatggtc tcgttggtgc
     5641 acacgtgcac gtaggccgcg cccggcgaga gcttccacga cgcctgcctg gggacatagg
     5701 tgaacttgcg atcctcggcg gtggccgcga ggttcgggcg gcagtacttg cgcgcctccg
     5761 agaacgcctt cttcgaccat tcgcccgtca cgacgaagtc ggcctcgctc ttgccgcgca
     5821 gcaggttcat cggcacggcc gcgaactgcg ccgtggcgcc gccctgcagg aagagcacct
     5881 tgtagttcgc ggggatcgcg aggagagcgc gaaggtccgc ctcggcctgc gcggcgatcg
     5941 ccacgaaatc cttgccgcga tggctcattt ccatcaccga catgcccctg ccgtgccagt
     6001 cgaggagctc cgccttcgcc tgctcgagga ccgccgggac gagcatcgcc gggccggatc
     6061 cgaaattcca cacgtgcatc gcctctgtcc ctccgagttt gcggaaccgt gccttcagcc
     6121 gcccgcctca ggcagcgggt gcatcgggga cgtcgtgtcc gttgccgttg ccgttgccgt
     6181 tgccgttgcc gttgccgttg ccgttctctt ccacgtcgcg gtccatcacc gtctgcaggc
     6241 ccgagagctt ctcgccctcg cccaggttga tgagcgtgac gccctgcgtg gaacggccca
     6301 tcacgcgcac ctccttcacg cgcgtgcgga tgagcacgcc gccggaggtg atgagcatga
     6361 tctcgtcgtc attcgacacc agggtcgcgg ccaccacctt gccgttgcgc gccgaggtgt
     6421 ggatggcgat catgccctgc gtgccgcgcc cgtggcgcgt gtactccgcg atcgaagtcc
     6481 gcttgccgta gccgttttcc gtggcggtga gaacactctg ctcttcgttc tcggccacca
     6541 gcagcgcgat gaccttgccg cccttggcga ggttcatccc gcgcacgccg cgggcgccgc
     6601 ggcccatcgg gcgcacgtcg ttctcgtcga agcgcaccgc cttgccctcg tcggagaaga
     6661 gcatcacgtc gtgcttgccg tccgtgagcg ccacgccgat gaggaaatca ccctcgtcca
     6721 ggtccacggc gatgatgccg gaagcgcgcg gccgcgagaa atcggaaagc gccgtcttct
     6781 tgaccacccc ttcctgcgtg gcgaagaaca cgtagcggtc ctcgacgaac tcgcgcaccg
     6841 ggaggatcgc gttgatgcgc tcgtcctggt ccatcttgag caggttcacg atcggcttgc
     6901 cgcggctggc tcggctgccc agcggcacct cgtagacctt gatccagtag accttgccct
     6961 tgttcgagaa gcagagcacg tagtcgtgcg tgttggcgat gaacagcttc tcgatgaagt
     7021 cgtcttcctt cgtggtggtc gcctgcttgc cgcggccgcc gcgcttctgc gcgccgtact
     7081 cgtcgagctt ctggctcttc acgtagcccg cgtgcgagag cgtcaccacg acgtcctcgg
     7141 gggcgatcag gtcctccatc gagaggtcct gcccgttcac cacgatctcg ctgcgccgct
     7201 tgtcgccgaa ctcgcgcttg atctccagca gctcgtcact gatgatgcgc gtgacccgct
     7261 ccggcttcgc gaggatgtcc aggaggtcct cgatcgccgc gatcacctcg cggtactcgg
     7321 agaggatctt gtcctgctcg agcccggtga ggcgctgcag ctgcatctcg aggatgcgtt
     7381 gcgcctggac gtccgagaga cggtagccgt tccccgcgag gccgtagtcc ttcggcaggt
     7441 tttcggggcg gaacaggtcg gatgtggcac gcgaaagcat gtcctcgacg agttgcgagc
     7501 gccaggtgcg cgacatgagc tcgaccttgg cgagcggggg cgtggcggcc gccttgatga
     7561 tggcgatcac ctcgtccacg ttggacagcg ccacggcgag gccttcgagc acgtggccgc
     7621 gctcgcgcgc cttgcgcagg tcgaacaccg tgcgccgcgt gaccacctcg cggcggtggc
     7681 gcaggaaggc atcgaggaac tgcttcaggt tcaggagctt cggctggccg tccaggaggg
     7741 cgaccatgtt catgccgaag gtctcctgca tctgagtgag cttgtacagg ttgttcagca
     7801 cgatctcggc cacttcgccg cgcttcagct cgatcacgag gcgcatgccg cccttgtcgc
     7861 tctcgttgcg caggtcggcg atgccctcga tcttcttttc gtgcaccagc tcgctcacgc
     7921 gcttgaggag ctggtcgccg cccacctggt agggcagctc gtcgacgacg atggcctgct
     7981 tgttcgcctt ctccatgtcc tcgatgtgtg cgcgcgcacg catcaccacg cgcccgcggc
     8041 cggtgcgata gccctccttc acctccgccg tgccgtagat gatgccggcg gtggggaagt
     8101 cgggcgcggg gatgatggcg atgagctcct cgatgtcgat gtcggggttg cccagcacgg
     8161 cgatgcaggc gtcgaccacc tcgttgaggt tgtgcggcgg gatgttggtc gccatgccga
     8221 ccgcgatgcc cgaggagccg ttcaccagca ggttcgggaa acgggccgga aggatcagcg
     8281 gctcggactc gctgccgtcg tagttggggc cgaagtcgac cgtttccttt tccaggtccg
     8341 ccagcagctc gtgagccacc ttggccatgc ggatctcggt gtagcgcatg gccgccgcgt
     8401 tgtcgccatc caccgacccg aagttgccct gcccgtcgat gagggggtag cgcaggctga
     8461 agtcctgggc catgcgcacg atcgtgtcgt agaccgcggt gtcgccgtgc gggtggtact
     8521 tgccgatcac gtcgcccacg atgcgggcgg acttcttgta ggcccggttc cagtcgttgg
     8581 acagctcgtg catcgcgtag agcacgcggc ggtggacggg cttgaggccg tcccgggcat
     8641 cgggaagcgc gcgccccacg atcacgctca tcgcgtaatc gaggtaggag cggcgcatct
     8701 cctgctcgat gctgatcggg tgtgtttcct tggcgaattg ttccatgtcg gggggattcg
     8761 aagcgaaccg gcccggcgcc gcagcgccga aacctaaccg cttgttttaa tgggatattt
     8821 gccggccggc aaaactgaaa tattagcaca ctcgaggcgc ttcccgccac ctgcgcgagg
     8881 tcgccggaag ggtgtggcgg aagtgcaacc cggggcctcc gggtgttgca caaaaccaac
     8941 gcttggttgt ggaccggagg ggggtttgtg tatgattgtg cgagttgttt caggcgcgag
     9001 cctaataaag atgcctggca tcaataaatc atccaaatct cttgggagtt ccaatatgaa
     9061 gatttcgtcg ttcgggaagc tgctgctcgg ttcagttgcc gccgcggcct tcgcctccgg
     9121 ctccgcgctc gctcagtccc cccagccgat cctcacggac agccagggcg taccggttcg
     9181 tgatgcttcc ggcaactgcg tgctgacatc tggaatcatc catcccgatt gcggggaaaa
     9241 gaaggaagcc cccgctccca agcctgccga gcccgccgca cccgctgcac ccgccgcgcc
     9301 cgccgccccg acgccgcccg ccccgaaacc ggccccggcc tcggtcaagc agacgatcgt
     9361 catccaggct gatgcgctgt tcgacttcga caagtcggtc gtccgcccgg atggccggaa
     9421 gaacatcgat gcagcgctgg ccaagctcgg cggcctcgac ctcgagatga tcatcgccac
     9481 cggccacacg gactcggtcg gcacggacgc ctacaaccag aagctgtccg agcgccgcgc
     9541 cgcagccgtg aaggcgtacc tggtctccaa gggcatcccg gcctcgaaga tcaccaccct
     9601 cggcaagggc gaatcgcagc ccgtggcgac caacaagacg aaggaaggcc gccagaagaa
     9661 ccgccgcgtg gacatcgagt tcaagggcgt caagaagtaa gcctcgccga acccggcaga
     9721 aaaccccgcc ccggcggggt tttttgttag cgagacgcca tgcgcgaagt cgataccctg
     9781 atccacgccg cctggatcgc ccccgtggag ccggcggggg tgctcctcga gcggcactgc
     9841 gtggccgtcc gcgacgggcg catcgaggcc ctgctgccct cggacgacgc gccgggcacc
     9901 ttcacggccc ggaccgaggt ccgcctcgac cggcacctcc tcatccccgg cctcgtgaac
     9961 ctgcacgggc acgccgcgat ggcgctgctg cgcggcatcg ccgacgacct gccgctcatg
    10021 acctggctga aggaccacgt ctggccggcg gaagcgaagc atgtcggcga cgagttcgtg
    10081 catgacggga gcctgcttgc cgccgccgag atgctgcgcg gcggcatcac ctgcttcaac
    10141 gacatgtatt tctttcccga ggccacggcg cgcgcggcgc tgaaggccgg catccgggcg
    10201 agcctcggca tgatcgcggt ggagtttccc agcgcctacg cggccgatgc caccggctac
    10261 ctgcacaagg gcctcgccac gcgcgacgcc taccggggcg aggaactgct gtccttctgc
    10321 ctcgcgccgc acgcccccta caccttggcc gacgagacgc tctcccgcat cgccaccctg
    10381 gccgaggaga tcgacgcgcc gatccacacg cacgtgcacg agacggccga cgagatcgcc
    10441 cagggcatcg cacagcacgg catgcgcccc ctggagcggc tgcgccgcct ggggctggtg
    10501 ggcccgcggc tcatcgccgt gcatgccgtc cacctcgagg aggccgagat cgacctcctg
    10561 gcgcacgaag gggcgtccat cgcccactgc ccgtcgtcga acctgaagct cgccagcggc
    10621 atc
//
LOCUS       DASUEV010000154         8000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1413266, whole genome shotgun sequence.
ACCESSION   DASUEV010000154 DASUEV010000000
VERSION     DASUEV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413266"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFV55_05090"
     CDS             <1..135
                     /locus_tag="VFV55_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX4943706.1"
                     /translation="RCEEGPVLVVYPEGTWYTYVDKSDIDEIIDEHLVNGRVVERLKI
                     "
     gene            132..797
                     /locus_tag="VFV55_05095"
     CDS             132..797
                     /locus_tag="VFV55_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4943707.1"
                     /translation="MSAVETRRATIEGSAGVIEIAYAIPAQPVAVAVVAHPHPLFGGA
                     MENKVVTTVARAFTDAGAATFRFNFRGVGASEGRHDEGRGETDDFLEVAAHAARAAGE
                     LPLWMAGFSFGGGVALRASARAEFACLVLVAPALRRITGHGLGEVPDANDPALGVPGR
                     HSSANTIIVHGDKDETVLLADSLAWGAVRDVPVLVVPGADHFFHRKLHVIRDVVGRLA
                     RGV"
     gene            complement(836..1273)
                     /locus_tag="VFV55_05100"
     CDS             complement(836..1273)
                     /locus_tag="VFV55_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX4943708.1"
                     /translation="MRLTTFTDYSLRVLMYVASHPEGRATIAEIAEAYDISEHHLTKV
                     VHFLGKEGYLENIRGRGGGLMLAREPASINVGEVVKRTEGGDIPAECFARETNTCAIT
                     TDCKLKHALADAVDSFYGALRKYTLEDVVRNRKALGKVLFARG"
     gene            complement(1295..3565)
                     /locus_tag="VFV55_05105"
     CDS             complement(1295..3565)
                     /locus_tag="VFV55_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEX4943709.1"
                     /translation="MQPTRNLWRWLAVVFVLSFAVLGWLGREIYLAAPPIATVKTTDG
                     ATLYTKEQIQDGQRAWLAAGGQQLGTVWGHGSYVAPDWSADWLHREALALRDIVADRL
                     YRSKFATLSAHQQASVDAIVKEQMRTNTYDAATNTLTIGTERAAAIVATQAHYEGLFG
                     SEPALDKLRDQYAMMPAALPRAEDRRALSGFFFWSAWSAATDRPGETGLSYTSNWPHE
                     PIVGNTLPASASIWTIVSIVLLIAGIAAMLWYHGANHEAADPAAPPKDPLLTLAPTPS
                     MKAVRKYFFVVIGLILVQIGMGAITAHYAVEGQAFFGLPLGEILPYTVSRTIHTQFGI
                     FWIATAWLATGLYIAPLLSGHEPKLQKLGVNALFYALLLIVVGSTATSWLGTLQRQGF
                     EFTFWVGNQGLEFTSMGRVWQILLFVGLLFWLFLMGRALWPALKRPSETRGLIAMVFL
                     SAACIGLFYSASLTWGQRTHYAMVEYWRWWLVHLWVEGFFEVFATAVVALIFTRLGLI
                     RASTANKAIVLETIVFLFGGILGTLHHLYWTGTPTSVIAVGAVFSALEVVPLALIGLE
                     GWNTYQRSKAAPWVSAYKWPILCFVAVGFWNVVGAGLLGFAINPPASLYYVQGLNLTP
                     AHGHAALFGVYGMLGIGLMLFCLRGLFERSLHADRYLAPAFWGLNAGLAMMVFMSLVP
                     AGIYQAWASITHGLWFARSAEMIHSPVMETLVWLRVPGDIVFAIGGVFLGVYAAALLR
                     RPRREVAQAAGEVQPT"
     gene            complement(3773..4405)
                     /locus_tag="VFV55_05110"
     CDS             complement(3773..4405)
                     /locus_tag="VFV55_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4943710.1"
                     /translation="MKSASRSFVVASAVATTVAAVAGLYSAAASANAVVGQPAPDFTA
                     TDLTGKPVSLADFKGKTVVLEWHNFGCPFVQKHYRSGNMQALQKKHGGEVVWLAVNST
                     NAGHSDYMEPAKLSRTLADFGAAPTRYVMDEPGRVGLAYGAKVTPHMYVIDPAGKVVY
                     NGAIDDKRSTDVDDVKTAKNFVVAALDELKAGKPVSNASNAPYGCTIKYR"
     gene            complement(4422..6593)
                     /locus_tag="VFV55_05115"
     CDS             complement(4422..6593)
                     /locus_tag="VFV55_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX4943711.1"
                     /translation="MTPRLHLSFAPFLLALSIAGAFVATAIHAAPVRTEHVEAELFAE
                     QVAATPGKPAPVGLRLRMDEHWHTYWKNPGDSGLPTKIRWTLPEGWSAGPIQWPHPEA
                     QRVGPLMNYGYSDEVMLLTDLTPPADAKPGPVAIRADATWLVCKDICIPEKATLALAF
                     AVAAGEPGAASANAALFARTRARLPVTPAGWISGSTLAGNKLTLRVMPPPGSTAPAKV
                     SFFPDRENFVDHPAPQAMVRDGNGFRLDVKLVDPVATGFKATSGVFVAESSWPGFPGR
                     KAVELSLPVAAAFPAIAAPVGAPAGEMNGSLAFALVFALAGGLLLNLMPCVFPVLGIK
                     VMGFVRHAHGDARALWIQGAVFSAGVLVSFLVLAGLLIALRAGGTELGWGFQLQSPAF
                     VTLLAALFFLMALNLFGVFEWGGFAQSMTSNLSAQGRYADAFLAGVLATVVATPCTAP
                     FMGAAVGFTLAQSAAVSLLVFATLGVGMALPVLALSFFPQALRKLPKPGPWMETFKQV
                     MAFPLFATVIWLAWVLGAQAGNDAILALLEGLLVLGIGAWIYGRWAHSESLVRFAFAA
                     LFAAAGLWLAWPDANATPGQARAASVKSGEIAWQEWSPERLAELRAAGTPVFVDFTAA
                     WCVTCQVNKRVALNRDEVVKALADRGVVALKADWTNYDPRITAALAELGRNALPVYAL
                     YAPGQSQPKLLPEVLTSSLVVDEISKLPAGKPATTANYSTR"
     gene            6723..7934
                     /locus_tag="VFV55_05120"
     CDS             6723..7934
                     /locus_tag="VFV55_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX4943712.1"
                     /translation="MSTLTRKRSAAPAPTVEVTRKESSDVQFFATCPRGLAGALAEEL
                     AGLGAKDIKAGDAGVDFAGPFDLAYAANLESRLATRILARVARFDYRNEKDIYDGAKS
                     VRWHAHFGVERTFKVDTNSVKAPVKSLDFITLKVKDAIADAFREALGKRPDVASRDPD
                     VRVHVFLDAKWCTLYLDTSGEPLFKRGRRDKTGEAPLKKNLAAGLLALSGWTPDMPLL
                     DPMCGAGTILIEAAEIALGLAPGRGRPFGFEKLTRFDAPAWDKVKAESSARAQAVGPR
                     AIHGSDLYGSTLDAARANLRDMGLEGVVSLKQANLLELSAPAPAGFLVTNPPYGVRLG
                     EKEELAKFYPELGHLLKQKFAGWNAYIFSGDPDLPKLIRLSASKRTVLYNGAIECRLY
                     EYRMVAGGNRK"
BASE COUNT         1371 a         2602 c         2814 g         1213 t
ORIGIN      
        1 cgctgcgagg agggcccggt gctcgtggtc tatccggaag gcacctggta cacctacgtg
       61 gacaagtcgg acatcgacga gatcatcgac gagcatctcg tgaacggccg cgtggtggaa
      121 aggctgaaga tttgagcgcc gtcgagacgc gccgcgcgac catcgagggt tccgcgggcg
      181 tcatcgaaat cgcttacgcg attcctgcgc aaccggtcgc cgttgcggtc gtggcgcatc
      241 cgcatccgct cttcgggggt gcgatggaaa acaaggtcgt caccaccgtg gcccgggcat
      301 tcacggacgc gggggccgcg accttccgct tcaatttccg cggcgtgggc gcgagcgagg
      361 gcaggcacga cgagggccgc ggggagacgg acgatttcct cgaggtggcg gcgcatgcgg
      421 cgcgcgcggc aggggagttg ccgttgtgga tggcgggatt ctcgttcggc ggcggcgtgg
      481 cgcttcgcgc cagcgctcgc gcggaatttg cctgcctcgt gctggttgcg cctgccttgc
      541 gtcgcatcac ggggcatggc ctcggtgaag tgccggacgc caacgatccg gcgctcggtg
      601 tcccgggccg tcattcttcg gccaatacga tcatcgtgca cggcgacaag gacgaaacgg
      661 tgctcctcgc cgattcgctg gcctggggcg cggtgcgcga cgtgcccgtg ctggtcgttc
      721 ccggcgccga ccacttcttc caccgcaagc tgcacgtcat ccgcgacgtc gtcggtcgcc
      781 tcgctcgcgg agtctgacac cgcccggtgt cacactccgt gcggtgtcag actcctcagc
      841 cgcgcgcgaa cagaaccttg cccaaggcct tgcggttcct caccacgtcc tcgagcgtgt
      901 acttgcgcag cgcgccgtag aaggagtcca ccgcgtcggc cagggcgtgc ttgagcttgc
      961 agtcggtcgt gatggcgcag gtgttggtct cacgcgcgaa gcactcggcc ggaatgtcgc
     1021 caccctcggt ccgcttgacg acctcgccga cgttgatcga ggccggttcc cgggcgagca
     1081 tgagcccgcc cccgcgcccg cggatgttct cgaggtagcc ttccttgccg aggaaatgca
     1141 ccaccttggt gaggtggtgc tcggaaatgt cgtaggcttc cgcgatctcc gcgatcgtgg
     1201 cgcgcccctc ggggtgcgac gccacgtaca tgagcacgcg caggctgtag tcggtgaagg
     1261 tcgtgaggcg cataacccgc atcctacatc cagcttaggt cggctgcacc tcgccggcgg
     1321 cctgcgccac ctcgcgccgg ggcctgcgca gcaaggcggc ggcgtacacg ccgaggaaca
     1381 cgccgccgat ggcgaacacg atgtcacccg gcacgcgcag ccagaccagc gtctccatga
     1441 ccggggagtg gatcatttcc gcggagcgcg cgaaccacag gccgtgcgtg atgctggccc
     1501 acgcctggta gatgcccgcg ggcaccagcg acatgaagac catcatcgcg agcccggcat
     1561 tgaggcccca gaaggcgggc gccaggtacc tgtcggcgtg caggctgcgc tcgaagaggc
     1621 cgcgcaggca gaagagcatg aggccgatgc ccagcatccc gtagacaccg aagagcgccg
     1681 catggccgtg ggccggcgtg aggttcaggc cctgcacgta gtagagcgag gccggcgggt
     1741 tgatcgcgaa gccgaggagc ccggcgccca cgacgttcca gaagccgacg gccacgaagc
     1801 acaggatcgg ccacttgtag gcggacaccc acggcgccgc cttcgagcgc tggtaggtgt
     1861 tccagccttc cagcccgatg agcgccagcg gcacgacttc gagcgccgag aacacggcgc
     1921 ccacggcgat gacggaagtg ggcgtgcccg tccagtagag gtggtgcagc gtcccgagga
     1981 tgccgccgaa gaggaacacg atggtctcga gcacgatcgc cttgttggcg gtggaagcgc
     2041 ggatgagccc caggcgcgtg aagatgagcg ccaccacggc cgtcgcgaac acctcgaaga
     2101 agccctccac ccagaggtgc accagccacc agcgccagta ctcgaccatc gcgtagtggg
     2161 tgcgctgtcc ccaggtgagg gaagcggaat agaaaagccc gatgcacgcg gccgacagga
     2221 acaccatcgc gatgaggccg cgggtttccg acgggcgctt gagcgcgggc cacagcgcgc
     2281 ggcccatgag gaagagccag aagagcaggc ccacgaagag caggatctgc cacacgcgcc
     2341 ccatgctcgt gaactccagc ccctggttgc cgacccagaa ggtgaactcg aagccctggc
     2401 gctgcagcgt tcccagccag ctcgtggcgg tggaacccac gacgatgagc aggagcgcgt
     2461 agaagagcgc gttcacgccc agcttctgca gcttcggctc gtggccggag aggagcggtg
     2521 cgatgtacag gccggtcgcg agccaggccg tggcgatcca gaagatgccg aactgcgtgt
     2581 ggatggtgcg gctcacggtg tagggcagga tctcgccgag cggcaggccg aagaacgcct
     2641 gcccctcgac cgcgtagtgg gccgtgatgg cgcccatgcc gatctgcacc aggatcaggc
     2701 cgatgacgac gaagaagtac ttgcgcacgg ccttcatgga gggcgtgggg gcgagcgtga
     2761 gcagcgggtc cttcggtggc gccgcggggt cggcggcctc gtggttggcg ccgtggtacc
     2821 agagcatggc ggcgatgccc gcgatgagga gcacgatgct cacgatggtc cagatcgagg
     2881 ccgaggcggg gagcgtattg ccgacgatcg gctcgtgcgg ccagttgctg gtgtaggaaa
     2941 ggcccgtttc gcccggccgg tcggtcgcgg cggaccaggc cgaccagaag aagaatcctg
     3001 aaagcgcgcg gcgatcctcg gcgcgcggca acgcggcggg catcatcgcg tactggtcgc
     3061 gcagcttgtc gagggcgggt tcgctgccga acaggccttc gtagtgcgcc tgcgtggcca
     3121 cgatggctgc ggcgcgctcg gtgccgatgg tgagcgtgtt cgtggcggcg tcgtaggtgt
     3181 tcgtgcgcat ctgctccttc acgatcgcgt cgacggaagc ttgctggtgc gccgagagcg
     3241 tcgcgaactt ggatcggtag aggcgatcgg ccacgatgtc gcgcagggcc agcgcctcgc
     3301 ggtgcagcca gtccgcggac cagtccggcg ccacgtagct gccgtggccc cacacggtgc
     3361 cgagctgctg tccgccggcg gccagccagg cgcgctggcc gtcctggatc tgctccttcg
     3421 tgtacagcgt ggccccgtcg gtggtcttca ccgtcgcaat gggaggcgcg gcgaggtaga
     3481 tctcgcgccc cagccagccg aggacggcga aggacaggac gaagacgacg gcgagccagc
     3541 gccagaggtt gcgggtcggt tgcatggcgg cattaccttt ccccccggag gggcttgtgg
     3601 cggaatatgg aatggatccg ttataaggtg tacatcatat gcagctttaa cgacgctgcc
     3661 ttgatttgaa tcaaatttcg accaatcggg acgcggaaat gcgaaggccc gctcgcggcg
     3721 ggccttcgct tcggcaggtg tacccggtac atgtaccggg tacagcgggg gtctaccggt
     3781 acttgatggt gcagccgtag ggtgcgttgg aagcgttgga gacgggcttg ccggccttga
     3841 gctcgtccaa ggccgcaacg acgaagttct tcgctgtctt cacgtcgtcc acgtcggtgg
     3901 agcgcttgtc gtcgatggcg ccgttgtaga ccaccttgcc ggcgggatcg atgacgtaca
     3961 tgtgcggggt caccttggcg ccgtaggcga ggcccaccct tccgggctcg tccatcacat
     4021 agcgcgtcgg cgcggcgccg aaatcggcca gtgtcctcga gagcttcgcg ggctccatgt
     4081 agtcggaatg gcccgcgttg gtggaattga ccgcgagcca gacgacctcg ccgccatgct
     4141 tcttctgcag ggcctgcata ttgccgctgc ggtagtgctt ctgcacgaac gggcaaccga
     4201 agttgtgcca ttcgaggacc acggtcttgc ccttgaagtc ggcgaggctc accggcttgc
     4261 cggtcaagtc ggtcgcggtg aagtcggggg cgggctggcc caccacggca ttcgcggaag
     4321 cggctgcgga atacaggccg gccacggcgg caacggtggt cgcgacggcg ctggcgacga
     4381 caaacgaacg ggaggcactt ttcatggcgg tctcctgggt atcagcgtgt cgagtagttg
     4441 gcggtggtcg ccggctttcc cgccggcagt ttcgagatct cgtcgacgac gagcgaggag
     4501 gtgagcacct ccggcagcaa cttcggctgg gactgcccgg gcgcatagag cgcatagacg
     4561 ggaagggcgt tgcggcccag ctcggcgagt gcggcggtga tgcgcgggtc gtagttggtc
     4621 cagtcggcct tcagcgcaac cacgccgcga tcggccagcg ccttcacgac ctcgtcgcga
     4681 ttgagcgcca cccgcttgtt cacctggcaa gtgacgcacc acgcggcggt gaagtcgacg
     4741 aacacgggcg taccggcggc gcgcaattcc gcgagtcttt ccggcgacca ttcctgccac
     4801 gcgatctccc cggacttcac gctggcggcc cgggcctgcc ccggcgtggc gttcgcatcc
     4861 ggccaggcaa gccacagccc ggcggcggcg aagagtgcgg cgaaggcgaa gcgcaccagg
     4921 ctttccgaat gggcccagcg gccatagatc cacgcgccga tgccgagtac caggagccct
     4981 tccagcagcg ccaggatcgc gtcgttgccg gcctgcgccc cgagtaccca ggcgagccag
     5041 atcaccgtgg cgaagagcgg gaaggccatc acctgcttga aggtctccat ccacgggccg
     5101 ggcttcggga gcttcctgag cgcctgcggg aagaaggaca gcgcaaggac gggaagcgcc
     5161 atgcccacgc caagcgttgc aaacaccaag agcgacaccg ccgcggactg cgccagcgtg
     5221 aacccgacgg cggcacccat gaacggcgcg gtgcacggcg tcgcgaccac ggtggcaagc
     5281 acgcccgcaa ggaaggcatc ggcgtagcgg ccctgggcgg aaaggttcga ggtcatcgac
     5341 tgcgcgaagc cgccccactc gaagacgccg aagagattga gggccatgag gaagaacagc
     5401 gccgcgagca gcgtcacgaa cgcgggcgat tgcagctgga agccccagcc cagctcggtg
     5461 ccgccggcgc gcagggcgat gagcagcccg gcgaggacaa ggaaggagac cagcaccccg
     5521 gccgagaaga ccgcaccctg gatccacagc gcgcgcgcat cgccatgggc atggcgaacg
     5581 aagcccatca ccttgatgcc gagcaccggg aagacgcagg gcatgaggtt gaggagcagc
     5641 ccgcccgcca gcgcgaagac cagcgcgaac gcgaggctgc cgttcatttc accggcgggc
     5701 gcgcccactg gagccgcgat cgccggaaac gcggccgcga cgggcagcga caattccacg
     5761 gccttgcggc cggggaaccc cggccacgag ctttcggcca cgaagacgcc ggacgtcgcc
     5821 ttgaaccccg tggccacggg atcgaccagc ttcacgtcga ggcggaatcc gtttccgtcg
     5881 cgcaccatcg cctgcggcgc ggggtggtcc acgaagttct cgcgatccgg aaagaagctc
     5941 accttggccg gcgcggtgga gcccggaggc ggcatcacgc gcaaggtgag cttgttgccc
     6001 gcgagcgtgg atccggatat ccacccggcg ggcgtgacgg gaaggcgcgc gcgcgtccgg
     6061 gcgaagaggg cagcattggc cgaagccgcg cccggctcgc ccgcggccac ggcgaaggcg
     6121 agcgcaagcg tggccttttc cggaatgcag atgtccttgc acaccagcca ggtggcatcg
     6181 gcccggatcg cgacggggcc gggcttcgcg tcggcgggcg gcgtgagatc ggtgagaagc
     6241 atcacctcgt cggaatagcc gtaattcatc agcggcccga cacgctgggc ctcaggatgg
     6301 ggccattgga tgggcccggc gctccaccct tccggcagcg tccagcggat cttcgtgggc
     6361 agtcccgagt cgcccgggtt cttccagtag gtgtgccagt gctcgtccat cctgagccgc
     6421 aggcccacgg gggcgggctt gcccggggtg gcggccacct gctccgcgaa gagctccgcc
     6481 tccacgtgct cggtcctcac cggtgcggcg tggatggccg tggccacgaa ggcgccggca
     6541 atcgacaggg ccaggaggaa gggagcgaag gaaaggtgca gtcggggcgt cacggtagaa
     6601 tccgggaaaa tcaaccgata gagcggcatt cgcgggggaa gttcattgct attctacttg
     6661 gcgcggctcc cggcccgcag tgcccgtgtc cctcgcggtc gagcaaccgt cccggaatgc
     6721 ccatgtccac gctgacccgc aagcgatccg ccgcgcccgc gcccaccgtc gaggtgacgc
     6781 gcaaggaatc gtccgacgtg cagttcttcg cgacctgccc gcgcggcctc gcgggcgctc
     6841 tcgccgagga actcgccggg ctgggcgcga aggacatcaa ggcgggcgac gcgggcgtgg
     6901 atttcgcggg gcccttcgac ctggcgtatg cggccaacct cgagtcgcgc cttgccacgc
     6961 gcatcctcgc gcgcgtcgcg cgcttcgact accggaacga gaaggacatc tacgatggcg
     7021 cgaagagcgt gcgctggcat gcgcacttcg gcgtggagcg cacgttcaag gtcgatacca
     7081 actcggtgaa ggcgccggtg aagagcctcg acttcatcac gctcaaggtg aaggacgcga
     7141 tcgcggacgc cttccgcgag gcgctgggca agcgccccga cgtggcctca cgcgatccgg
     7201 acgtgcgcgt gcacgtattc ctcgacgcga agtggtgcac gctctacctc gacaccagcg
     7261 gcgagccgct cttcaagcgc ggccgtcgcg acaagacggg cgaggcgccg ttgaagaaga
     7321 acctggccgc gggactgctc gccctttccg ggtggacacc ggacatgccg ctcctcgatc
     7381 cgatgtgcgg cgcaggcacg atcctcatcg aggctgcgga gattgcattg ggactggcac
     7441 ccggccgcgg ccgccccttc gggttcgaga agctcacgcg cttcgacgcc ccggcgtggg
     7501 acaaggtgaa ggccgagtcc tcggcccggg cgcaggccgt ggggccgcgg gccatccacg
     7561 gctccgacct ctacggaagc acgctcgatg ccgcgcgcgc gaacctgcgc gacatgggcc
     7621 tcgagggcgt cgtctcgctc aagcaggcca acctcctcga gctttccgca ccggctccgg
     7681 ccggtttcct cgtcaccaac ccaccgtacg gcgtgcgcct gggcgagaag gaagagctcg
     7741 caaagttcta tccggagctc ggccatctcc tcaagcagaa gttcgcgggc tggaacgcct
     7801 acatcttctc cggcgacccg gacctgccga agctgatccg gctttccgcc agcaagcgca
     7861 cggttctcta caacggtgcc atcgagtgcc ggctctacga gtaccggatg gttgccggcg
     7921 gcaaccgcaa gtgagtcgcg gaaaaggggt caggttactt tgcacaaaaa acaggcagca
     7981 aatatttgct gcctgttttt
//
LOCUS       DASUEV010000155         4665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1076741, whole genome shotgun sequence.
ACCESSION   DASUEV010000155 DASUEV010000000
VERSION     DASUEV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076741"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /gene="nuoL"
                     /locus_tag="VFV55_05125"
     CDS             <1..1542
                     /gene="nuoL"
                     /locus_tag="VFV55_05125"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX4943713.1"
                     /translation="FFTYLNMFVFSMVMLVLAGNFLVLYVFWEAVGLCSYLLIGFWYE
                     KKAASDAGKKAFIVNRVGDFGFGLGIMLIWVTFGTLDYGEVFAKADASVSTNVYLAIA
                     LLLFIGACGKSAQLPLFTWLPDAMEGPTPVSALIHAATMVTAGVYMVARCHTLFEMAP
                     LSLEIVAWVGGLTAVFAATIGLVQTDIKRVLAYSTISQLGYMFVGVGVGAYAAGVFHL
                     VTHAFFKALLFLGAGSVIHGLGGEQDLRKMGGLAPRMMTTTVTFLIGAFGLAGVPPLA
                     GFFSKDEILASVFHEHHYVLWVLLIAGAFMTAFYTFRLVFLAFFGAPRMSKEVAHHIH
                     ESPASMTVPLIILAVATVVAGLAFGIPSSTGTPFARFLAPVLPLEEGEHGAGIAFTLL
                     LLSAVIAIAGVALAWFVYGRSPVRVASIGVPRNPVHRLLLEKYYVDELYDLLFVRPIY
                     NLSLWLARVFDPRVVDGLVNGSATAVLAWARGLRLVQTGFVMNYALGILVGAVAMVAY
                     LLARR"
     gene            1548..3065
                     /locus_tag="VFV55_05130"
     CDS             1548..3065
                     /locus_tag="VFV55_05130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX4943714.1"
                     /translation="MAYLLSLVTFLPVVGAVLVLLLPRRQEHVTKLVTLGATLVTFVI
                     SLPLYFRFDPTSADYQFVEQKVWIPSLGASYHLGVDGISVLLVLLTTFLMPLVILSSW
                     ASVESRWKEFAVTMLLLEAGMVGVFVALDLFLFYVFWEAMLIPMYLIIGIWGGNNRIY
                     AAVKFILYTLAGSLLMLVAILALYFQHGAATNVYTFDLPVLARYVLPGGRVQDLMFFA
                     FALAFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFCLPLFPDASL
                     HYAPWMFTLAVIGIIYGAWVSTVQPDIKKLVAYSSVSHLGFVVLGLFTLTAQGMVGGL
                     IQMVSHGLSTGALFLMVGMIYERRHTRLIADFGGLWKVIPAFSVIFLVVCLSSVGLPG
                     LNGFVGEFLILLGAFQVNRLSAVLATSGIIFAAVYLLWMYQRVCFGEVTHEANRRLTD
                     LSLREWVVLLPVLLFIFWIGVYPTTFTAMTEPSVQALIAQVQSKAQATASAMVTR"
     gene            3062..4543
                     /locus_tag="VFV55_05135"
     CDS             3062..4543
                     /locus_tag="VFV55_05135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX4943715.1"
                     /translation="MNPALVLPVVDLRPLLPMLVLAATAVVVILLDLLPPRERKDHLG
                     FVSALGVVLTLIMTYWMTFSIGGGELRGFRGMVVLDPYALFFNVVIGYATGLVILLSM
                     DYIRREGQEAGEFYILVLLSSLGMMLMASAGDLIIVFLGLETMSIALYVLTGFFRHRL
                     DAGEASMKYFLMGAFASGFFLYGIALIFGATGSTNLDRIANAVAAGAGRDPMLVIGFG
                     LLLVGFGFKISSVPFHMWAPDAYEGAPTSITAFIATGSKAAAFASLLRVLMTALRGAP
                     LDWPMLMWGMAAVTMTVGNVVALAQQNIKRMLAYSSIAHAGYLLVALVAHSDVGTSAF
                     LFYLAAYTLMTLGAFGIVIVLGEKGTENTSISRDYAGIAWRHPLLAAAMALFMFSLTG
                     IPPTAGFVGKFAILSAAVRAGHVPLAILLVLASVISAYFYLKVVVSMYMWTSDRVPIR
                     SRVPAMAAIALLIVVVGVLGLGIFPGSWVDLARSSSTGLPGLP"
BASE COUNT          550 a         1622 c         1544 g          949 t
ORIGIN      
        1 ttcttcacct acctgaacat gttcgtgttc tcgatggtga tgctggtgct ggccggcaac
       61 ttcctcgtcc tctacgtctt ctgggaggcg gtgggcctct gctcctacct cctgatcggc
      121 ttctggtacg agaagaaggc ggcctccgac gcgggcaaga aggccttcat cgtcaaccgg
      181 gtgggcgact tcggcttcgg gctcggcatc atgctgatct gggtgacctt cggcacgctc
      241 gactacggcg aggtcttcgc caaggccgac gcgagcgtga gcaccaacgt ctacctggcc
      301 atcgcgctcc tcctcttcat cggcgcgtgc ggcaagtcgg cgcagctccc gctcttcacc
      361 tggctgcccg acgcgatgga agggccgacg ccggtctccg ccctcatcca cgccgccacc
      421 atggtgaccg cgggcgtgta catggtggcg cggtgccaca cgctcttcga gatggcgccg
      481 ctgagcctcg agatcgtggc gtgggtgggc ggcctcaccg cggtgttcgc ggccaccatc
      541 ggcctcgtgc agaccgacat caagcgcgtg ctcgcctact ccaccatcag ccagctgggc
      601 tacatgttcg tgggcgtggg ggtcggcgcg tacgcggcgg gcgtcttcca cctggtgacg
      661 cacgccttct tcaaggccct gctcttcctg ggggcgggga gcgtcatcca cgggctgggc
      721 ggcgagcagg acctgcgcaa gatgggcggg ctcgccccgc gcatgatgac cacgaccgtc
      781 acgttcctga tcggggcctt cggcctcgcg ggggtgccgc cgctggccgg gttcttctcc
      841 aaggacgaga tcctcgcctc cgtgttccac gagcaccact acgtgctgtg ggtgctcctg
      901 atcgcgggcg cgttcatgac cgccttctac acgttccgcc tcgtgttcct cgccttcttc
      961 ggcgcgccgc gcatgtcgaa ggaggtggcg caccacatcc acgagtcgcc ggccagcatg
     1021 acggtccccc tgatcatcct ggccgtcgcc accgtggtgg cgggtctggc cttcggcatc
     1081 ccgtcctcca ccggtacgcc gttcgcgcgc ttcctcgccc cggtgctgcc gctcgaggag
     1141 ggcgagcacg gcgcggggat cgcgttcacg ctcctgctgc tctccgcggt gatcgccatc
     1201 gcgggggtgg cgctggcgtg gttcgtctac ggccgctcgc cggtgcgggt cgcgtcgatc
     1261 ggcgtaccgc ggaacccggt gcaccggctg ctgctcgaga agtactacgt ggacgagctc
     1321 tacgatcttc tcttcgtgcg accgatctac aatctctcgc tctggctcgc gcgcgtcttc
     1381 gatccgcgtg tcgtcgacgg gctcgtcaac gggtccgcga ccgcggtgct ggcctgggcg
     1441 cgcggcctgc gcctggtgca gaccggcttc gtgatgaact acgcgctcgg catcctggtg
     1501 ggcgcggtgg ccatggtggc ctacctcctg gcccgccgct agcccgcatg gcctacctgc
     1561 tctcgctcgt caccttcctg ccggtggtgg gcgccgtgct cgtcctgctg ctgccgcgcc
     1621 gccaggagca cgtgaccaag ctggtgacgc tgggcgccac cctggtcacc ttcgtgattt
     1681 cccttccgct ctacttccgc ttcgacccga cctccgcgga ctaccagttc gtggagcaga
     1741 aggtgtggat cccctcgctg ggggcgagct atcacctggg cgtggacggc atcagcgtgc
     1801 tgctggtgct gctgaccacg ttcctgatgc cgctggtgat cctgtcctcg tgggcgtcgg
     1861 tggagtcgcg gtggaaggag ttcgcggtca ccatgttgct gctggaagcg gggatggtcg
     1921 gggtcttcgt cgccctcgac ctcttcctct tctatgtgtt ctgggaggcg atgctgatcc
     1981 cgatgtacct catcatcggg atctggggcg ggaacaaccg gatctacgcg gcggtgaagt
     2041 tcatcctcta tacgctggcc ggctcgctcc tgatgttggt cgccatcctg gcgctctact
     2101 tccagcacgg cgccgccacc aacgtctaca cattcgacct gccggtgctc gcgcgctacg
     2161 tgctgccggg cgggcgcgtg caggacctga tgttcttcgc gttcgcgctt gccttcgcga
     2221 tcaaggtgcc gatgttcccg ttccacacgt ggctcccgga cgcccacgtg gaggcgccca
     2281 ccgcgggcag cgtgatcctg gccggcgtgc tcctgaagat ggggacctac ggcttcctgc
     2341 gcttctgcct gccgctcttt ccggacgcga gcctgcacta tgcgccgtgg atgttcacgc
     2401 ttgcggtcat cggcatcatc tacggggcgt gggtctccac cgtgcagccc gacatcaaga
     2461 agctggtggc ctactccagc gtgagccacc tgggcttcgt ggtcctcggg ctcttcaccc
     2521 tcaccgcgca ggggatggtg ggcggcctca tccagatggt gagccacggg ctcagcacgg
     2581 gcgcgctctt cctcatggtg ggcatgatct acgagcggcg gcacacccgg ctcatcgccg
     2641 acttcggcgg gctgtggaag gtgatccccg cgttcagcgt catcttcctg gtggtgtgcc
     2701 tgtcctcggt ggggctgccc gggctcaacg gcttcgtggg cgagttcctc atcctgctgg
     2761 gcgccttcca ggtcaaccgg ctctccgcgg tgctcgccac ctccggcatc atcttcgcgg
     2821 cggtctacct gctgtggatg tatcagcgcg tgtgcttcgg cgaggtcacc cacgaggcga
     2881 atcgccggct gaccgacctc tccctccgcg agtgggtggt gctcctgccc gtgctcctgt
     2941 tcatcttctg gatcggcgtc tacccgacca cgttcaccgc gatgaccgag ccgagcgtgc
     3001 aggcgctgat cgcccaggtg cagagcaagg cgcaggcgac cgcctccgcc atggtgacgc
     3061 ggtgaacccg gcgctcgtgc tgccggtggt ggacctgcgg cccctcctgc ccatgctggt
     3121 gctggccgcc accgcggtcg tggtgatcct gctcgacctg ctgcccccgc gggagcgcaa
     3181 ggaccatctg ggcttcgtga gcgcgctcgg cgtggtgctg acgctgatca tgacgtactg
     3241 gatgacgttc tcgatcggtg ggggcgagct gcgcggcttc cgcggcatgg tggtgctcga
     3301 tccctacgcg ctcttcttca acgtggtgat cggctacgcg accgggctcg tcatcctgct
     3361 ctcgatggac tacatccgcc gcgagggcca ggaggcgggc gagttctaca tcctggtgct
     3421 gctctcctcg ctcggcatga tgctgatggc ctccgcgggc gacctgatca tcgtcttcct
     3481 cggcctcgag acgatgtcca tcgcgctcta cgtgctcacc ggcttcttcc gccaccggct
     3541 cgacgcgggc gaggcctcga tgaagtactt cctgatgggc gccttcgcct ccggcttctt
     3601 cctctacggc atcgcgctca tcttcggggc caccgggtcc accaacctcg accgcatcgc
     3661 caacgcggtg gccgcggggg cgggccgcga tccgatgctg gtgatcggct tcggcctcct
     3721 cctggtgggc ttcggcttca agatctcctc ggtgccattc cacatgtggg cgcccgacgc
     3781 gtacgagggc gcgcccacct cgatcaccgc cttcatcgcc accggctcca aggcggccgc
     3841 cttcgcttcg ctgctgcgcg tgctgatgac cgcgctgcgc ggcgcgccgc tcgactggcc
     3901 gatgctcatg tggggcatgg ccgcggtcac catgacggtg ggcaatgtgg tggcgctcgc
     3961 tcagcagaac atcaagcgca tgctcgccta ctcctcgatc gcccacgcgg gctacctcct
     4021 ggtggccctc gtcgcgcaca gcgacgtggg aacctcggcg ttcctcttct acctcgcggc
     4081 gtacaccctg atgacgctgg gcgcgttcgg gatcgtgatc gtcctggggg agaaggggac
     4141 cgagaacacg tcgatctcgc gggactatgc cggcatcgcg tggcggcacc cgctgctggc
     4201 ggcggccatg gccctgttca tgttcagcct gaccggcatc cctccgaccg cgggcttcgt
     4261 cggcaagttc gcgatcctgt cggccgccgt ccgggccggg cacgtgccgc tcgccatcct
     4321 gctcgtcctg gcctccgtca tctcggcgta tttctacctc aaggtcgtgg tctccatgta
     4381 catgtggacg tcggaccggg tgccgatccg ctcccgcgtt cccgccatgg cggcgatcgc
     4441 gctgctgatc gtcgtggtgg gcgtgctcgg cctggggatc ttcccgggga gctgggtcga
     4501 tctcgcgcgg tcgtcgtcga ccggcctgcc gggcctgccg tgaggcgcct ggtggggctc
     4561 gccctcgcgg gcgtcgtcgc gctgggcggt tgcagcgacg gcgcctccga agacggcgcg
     4621 gcggtgggga cggccggcgc gatcgaggtg gcgcactttc cgctc
//
LOCUS       DASUEV010000156        11377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1076786, whole genome shotgun sequence.
ACCESSION   DASUEV010000156 DASUEV010000000
VERSION     DASUEV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076786"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFV55_05140"
     CDS             complement(<1..703)
                     /locus_tag="VFV55_05140"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX4943716.1"
                     /translation="MNELQIGLLILVVTTVVLFSGFPIAWGLALVSVVFLFAFKGPAS
                     LAVIATQFMDELNSFALLTIPLFILLGASIGVSAAGKDIYNSLYRWLARVPGGLIIAN
                     ILACGMFSAVCGSSPATAAAIGKAGVPEMIRRGVPPSLATGAICAGGTLGILIPPSIT
                     FILYGLATETSIGRLFLAGVVPGACLVLLFSAWAWFASLVARRELIVVDEHFTLKEKM
                     EGMVRVGPFLAIIAAI"
     gene            complement(707..1222)
                     /locus_tag="VFV55_05145"
     CDS             complement(707..1222)
                     /locus_tag="VFV55_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX4943717.1"
                     /translation="MRNFVRFVDRLSDACAVAAAFMLVAAMLIVCWMVVYRALGNSTY
                     WEIELATYLIVATVLVGSPYCLKTRGHIGVDLVSEWLSPRGRRILARILAALGLAVCL
                     YLTWKGLELTVEAWQKGEHSGSTWNPLRWPFFAMMPLGLGLTALQYVADFLRSDPDAA
                     DAPAPISGQGA"
     gene            complement(1302..2297)
                     /gene="dctP"
                     /locus_tag="VFV55_05150"
     CDS             complement(1302..2297)
                     /gene="dctP"
                     /locus_tag="VFV55_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX4943718.1"
                     /translation="MNNRIRQGALALIAAAALAMPALAQEIKISHQFKANTDGRDKAT
                     RVFVEEVNKKDPSLKFRIYPGSSLQIKPVAQFDALQSGTLEMAVFPMSYAVGKVPEMS
                     ITIMPGTITSIDHAMRLKGTAFHKKLQEVAEKNGVHIVTWWWTPGGFASKIHEIGGPS
                     TVKGEKMRAADPTFESMLKAAGASVLNIPSTEIYPSLQSGVLTSTLTSAETFVSMRIY
                     EQTKFATVGGDYTLWWLLQPLVMSKAAWDKLTPAQKKIFEEAADKSDTFFAAGQREAV
                     KHMVDAYTKAGAKVRALTKAEFDDWIDLAKKTSWPEYSAISPDAKQLLSLITAVK"
     gene            2506..5406
                     /locus_tag="VFV55_05155"
     CDS             2506..5406
                     /locus_tag="VFV55_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4943719.1"
                     /translation="MTTTHSVRFSSDRNAPAHPIAERLRREVDGEVLFDRASRGRYAT
                     DASIYQIEPVGVVVPRSLDAAVSALQIAAEAGVPILPRGGGTSQGGQTVGAALVIDAS
                     KYLNRLVEVDPVARRAIVEPGLVLDHLNAQLRRDGLWFPVDVSTAAQATLGGMTGNNS
                     CGSRSMAYGYMVHRVAAIDACLADGTRGRFGPAAELVHPAIRALSDRAAALWAREKDE
                     IAARMPKVARRVAGYNLDRLAPGELNLAKLLVGSEGTLAWFERITLDLADIPKHKALG
                     VAHFPRFYDAMDATQHIVKLGPCAVELVDRTMIELARAIPAFAATIGAFVRGEPDAIL
                     LVEFAGEDRDEQVRGVKRLAELMADLGFPGGVVEILDAGRQKELWEVRKAGLNIMMSM
                     KGDGKPVSFIEDCAVPLEHLAEYTSKLTEVFRKHGTEGTFYAHASVGCLHVRPILNMK
                     GDGTIRMRAIAEEAADLVRRYKGAYSGEHGDGLVRSEWIAPFFGPRLTAALGEIKGWF
                     DPRGLMNPGKIVNPPKQDDRSLLRYKPGYHAEAPATALDWSAWGGLPGAVEMCNNNGH
                     CRKFDAGTMCPSYRVTRDEVHVTRGRANTLRLALSGQLGEDGLASDAVRDAFDLCVSC
                     KGCRRECPTGVDMARMKIEFLNHDRARNGHSVRDRLVGMLPAYAPWASRFAPLVNAAQ
                     RMPGVSRALHALAGFDSRRELPAFSARPWLAAQPPYVARGGREVVLFADTFNNWFEPG
                     NLAAAQRVISAAGFEVVPARGDDARPLCCGRTYLSAGMVDRAKAESRRMIDALAPWIE
                     RGVPVVGLEPSCLYSLKDEFLAMHPGDSRATALAELAVTFESFIARALRDGARIAWRE
                     GHEPEFLVHGHCHQKAFGSFDDTLAALRAIPGARVSAVESSCCGMAGSFGHEHYDVSM
                     AMGEASLLPAVRAAAPATVVVAAGTSCRHQIDHGASREAVHPAVALAAALAP"
     gene            5412..6656
                     /locus_tag="VFV55_05160"
     CDS             5412..6656
                     /locus_tag="VFV55_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX4943720.1"
                     /translation="MPSSAPVVEALRAALDFTPLGEPAGNVTLGKGTLDGRPVRVALV
                     ENRIASGSIGKAEVAKLVPLFSIAAREKSAVVLYLDSAGARISQGLEALGAFRRLFRE
                     ALAARIAGASIAVVLGRNCYGGASMLAHVGAKRLFSPGTQLAMSGPSILARSAGVDAL
                     DEMFRAMADAAIGAAARAKASEANAVWTPGMDLPAWLRVALAPDAGPWNAFLARHQLL
                     RTRLDKGLTTRQPENVRRKDLEKLFAQGYRAIECDGVITGEATCDGVAVPVLGLVGPN
                     HVGAERAWRFADAAWRMAKGAPARVEVLLDCESHAARLDDERAVLTEFIVDMGVALAA
                     VAARGTHVELTILDRAGGGVYVALAAPATHVSVVFGADIQVLPGAAVASILGSHRDAV
                     GDISDYRRAGVADAEHKLGLPP"
     gene            6665..8182
                     /locus_tag="VFV55_05165"
     CDS             6665..8182
                     /locus_tag="VFV55_05165"
                     /inference="COORDINATES: protein motif:HMM:NF005702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEX4943721.1"
                     /translation="MNENLYSLFESRFPADRTRPLLILPDGREISYGQAEESCARFAA
                     FLAGRGLSPGDRVAVQVEKSPEALLLYLACLRAGLVYLPLNSAYQAGEVGYFLDNAQA
                     GAVIAQPRSLPWLEPLAAKLGIRHVFSLDEHGKGSFVEAAHGAGSRFDTVARRADDVA
                     AILYTSGTTGRSKGAMITHRNLASNAQVLHAYWGFRPGDILVHMLPLFHVHGLFVACH
                     CVLMNGTAMRFHAKFDAPLAIADFGRSTVFMGVPTLYTRLLAEAGLDRKACAGMRLFV
                     SGSAPRLAETHAQFEERTGHRILERYGMTETGMLVSNPLEGERRPGTVGLPLPGTSVR
                     VVDDTGRPCTAGEIGHIQVKGDNVLPGYWRMPERNKEEFTTDGYFRTGDVGSFSEDGY
                     LAIVGRSKDLIITGGYNVYPKEVELAIDELPAVAESAVIGVPHPDFGEAVTAIVVPRT
                     GAQAPTEDEVIAWLKSRLANFKVPKRVFVAQELPRNTMGKVQKNVLRDRYSSVGS"
     gene            8185..9504
                     /locus_tag="VFV55_05170"
     CDS             8185..9504
                     /locus_tag="VFV55_05170"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEX4943722.1"
                     /translation="MPGTRTILDRLVGTFAAATRSRSERRAIRLAKLLRTLISQRGES
                     SGALMARRAVALYNGLDESGRRLFFDLLARDFSPDPDEVLAAAQAYQREPVPATLARL
                     QRVAEPPRQEVFRRMNMSPNGTAVLVGMRKVLLEALPANPQFASLDDDLTHLFGSWFN
                     RGFLELRRIDWGTPASILEKLIAYEAVHEIQGFPDLKRRLAHDRRCFAFFHPALPGEP
                     LVFVEVAFVDELPSAVAPILSQESAVGDPRKARCAVFYSITSCQPGLRGVSFGNFLIK
                     QVAQDLHEELPNLRVFATLSPMPGFRAWLRRQPDDRVLAAAPPIRFAAQADPARAPGQ
                     ELREPLQALAAVYLAKQWDQGHVLDPAARFHLGNGARLERINWLADTSARGLHQSLGL
                     MANFVYALDDVERNHESYVNRHVVACSPAVERLVRFAEGDVKGAPSA"
     gene            9556..9999
                     /locus_tag="VFV55_05175"
     CDS             9556..9999
                     /locus_tag="VFV55_05175"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX4943723.1"
                     /translation="MKVFRLSCAHGHDFEGWFASSEDFDRQQAGGEISCPLCENRSVA
                     KLPSAPYVNTGARGTQTMSVAAPATPATPDLSVALAALRAYVSANTENVGRQFPEVAR
                     RMHYGEESQRGIRGRVTTEEAVALREEGIEAIPLPPGLALDEKIH"
     gene            complement(10021..11205)
                     /locus_tag="VFV55_05180"
     CDS             complement(10021..11205)
                     /locus_tag="VFV55_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4943724.1"
                     /translation="MKPSDPKPIFQWDDPFLLDAQLTEEERMVRESALAYSQEKLKPR
                     ILEAHRHERFDRGILNEMGELGFLGSTIEGYGCAGVNNVCYGLIAREVERVDSGYRST
                     MSVQSSLVMHPIHAYGTEAQRQKWLPRLATGELVGCFGLTEPDHGSDPGGMVTRARKV
                     DGGWRVSGAKMWITNSPIADLFVVWAKDDAGDIRGYLLEKGMKGLSAPKIEGKFSLRA
                     SITGEVVMEDVFVPDDNYLPNVKGLKGPFGCLNKARFGISWGALGAAEFCWHAARQYT
                     LDRHQFGKPLAANQLIQKKLADMQTEIAIGLQAVLRVARLLDEGRATPEMVSLVKRNS
                     CGKALDIARTARDMHGGNGIADEYHVIRHVLNLEAVNTYEGTHDVHALILGRAQTGIS
                     AF"
BASE COUNT         1754 a         3854 c         3939 g         1830 t
ORIGIN      
        1 cgatcgccgc gatgattgcg aggaacgggc cgacgcgcac catgccttcc atcttttcct
       61 tcagcgtgaa gtgctcgtcg accacgatga gctcccggcg ggcgaccagc gaggcgaacc
      121 acgcccatgc ggaaaacagg agaacgaggc aggcgcctgg aaccacgccc gccaggaaca
      181 gccggccgat cgaggtctcc gtcgccagcc cgtacaggat gaaggtgatg gacggcggga
      241 tgaggatgcc cagcgtgcct cccgcgcaaa tcgccccggt ggcgagcgag ggcggcacgc
      301 cgcgccggat catttccgga acgccggcct tgccgatggc ggcggccgtg gccggcgagg
      361 aaccgcaaac cgccgagaac atgccgcagg ccaggatgtt ggcgatgatg agccccccgg
      421 gcacgcgcgc cagccatcgg tacagggagt tgtagatatc cttgccggcg gcggacaccc
      481 cgatggaggc acccagcagg atgaagaggg gaatggtgag gagcgcgaag gaattgagct
      541 cgtccatgaa ctgcgtggcg atcaccgcga ggctcgccgg gcccttgaag gcgaaaagga
      601 aaacgaccga gacgagcgcg agtccccagg cgatcgggaa gccggagaag agcaccacgg
      661 tcgtgacgac caggatgagc aggccgatct gcagttcgtt catgcctcag gccccctggc
      721 cggatatcgg ggccggggcg tcggccgcat ccgggtcgct gcgcaggaag tcggccacgt
      781 attgcagcgc ggtgagcccc agccccaggg gcatcatggc gaagaagggc cagcgcagcg
      841 gattccacgt cgagccgctg tgctcgccct tttgccacgc ctccaccgtg agttcgagcc
      901 ccttccacgt caggtagagg cagaccgcca gcccgagcgc cgcgagaatg cgcgcgagga
      961 tgcgccgccc gcgtggcgaa agccattccg agacgagatc gacgccgatg tgtccgcggg
     1021 tcttcaggca gtaaggcgag ccgacgagga ccgtggccac gatgagatag gtggccagct
     1081 cgatctccca gtaggtcgag ttgccgagcg cgcggtagac caccatccag cagacgatga
     1141 gcatggcggc cacgagcatg aaggcggcgg cgactgcgca ggcatcggac aggcgatcga
     1201 cgaagcggac gaagttgcgc acgatgcttc ctggcggccg gcgggccgcg gcaatgggtc
     1261 ttgggcagcc gatcggggcg gcgcgagccg ccccgcgagc ctcacttgac cgcggtgatc
     1321 agggagagga gctgtttcgc gtccggcgat atcgcggagt actcgggcca cgatgtcttc
     1381 tttgccaggt cgatccagtc atcgaattcc gccttcgtga gcgcgcgaac cttggcgccg
     1441 gccttggtgt aggcatcgac catgtgcttc accgcctcgc gctggccggc ggcgaagaag
     1501 gtgtcgctct tgtcggcggc ctcctcgaag atcttcttct gcgcgggcgt gagcttgtcc
     1561 caggcggcct tggacatgac cagcggctgc agcagccacc agagggtgta gtcgccgccc
     1621 acggtggcga acttcgtctg ctcgtagatg cgcatgctga cgaaggtctc ggccgaggtg
     1681 agggtcgagg tgagcacgcc cgactgcagc gaggggtaga tctccgtgga gggaatgttg
     1741 aggacggaag cgcccgcggc cttcagcatg ctctcgaagg tcgggtcggc cgcgcgcatc
     1801 ttctcgccct tgacggtact gggcccgccg atctcgtgga tcttggaggc gaagccgccc
     1861 ggcgtccacc accaggtgac gatgtgcacg ccgttcttct cggcgacttc ctggagcttc
     1921 ttgtggaagg cggtgccctt caggcgcatc gcatggtcga tgctcgtgat ggtgcccggc
     1981 atgatggtga tcgacatttc cggcactttc ccgaccgcat aggacatcgg gaagaccgcc
     2041 atctccaggg tgcccgactg cagcgcgtcg aactgggcga cgggcttgat ctgcagcgag
     2101 ctgcccgggt agatgcggaa cttgaggctc gggtccttct tgttcacctc ttccacgaac
     2161 acccgggtcg ccttgtcgcg cccgtcggta ttggccttga actggtgcga gatcttgatt
     2221 tcctgcgcga gcgcgggcat ggccaatgcg gccgcggcga tgagcgccaa cgcgccctgc
     2281 cggatgcggt tgttcatggg ggtctcctca ggtcttgatt tgtcacaggt gccgtctcgg
     2341 tgcggcagtg ccgggaaacc tacgcgatag ggacggcagt tgtaaaccgc gccggtcaag
     2401 cctccttcgc aatgctcgcg catgtcattg cggcggaccg gctatttctg cccataatgc
     2461 cgcagccggc cgcctcacgc ccatcgcggc ccgatcgacg atccgatgac taccacgcat
     2521 tccgtccgct tttcctccga tcgcaacgcg cccgcccacc ccatcgccga gcgccttcgc
     2581 cgggaggtgg acggcgaagt gctcttcgac cgcgccagcc gcggccgcta tgcgacggac
     2641 gcgtcgatct accagatcga gccggtgggt gtcgtcgtgc cgcgttccct cgatgcggcg
     2701 gtcagcgcgc tgcagatcgc cgcggaggcg ggcgtgccga tcctgccgcg cggcggcggc
     2761 acctcgcagg gcgggcagac ggtgggcgcg gccctggtca tcgacgccag caagtacctc
     2821 aatcgcctcg tcgaggtgga ccccgttgcg cggcgcgcca tcgtggagcc ggggctcgtc
     2881 ctcgatcacc tcaacgcgca gttgcgccgc gacggattgt ggttccccgt ggacgtctcg
     2941 acggcggccc aggcaaccct cggcggcatg acgggcaaca actcctgcgg gtcgcgctcg
     3001 atggcctacg gctacatggt ccaccgcgtc gcggccatcg atgcctgcct tgccgacggc
     3061 acgcgcggcc ggttcgggcc cgcagcggag ctggtgcacc cggccattcg cgcgctttcg
     3121 gaccgggcgg cggcgctgtg ggcccgcgag aaggacgaga tcgcggcgcg aatgccgaag
     3181 gtggcgcgcc gcgtggcggg atacaacctc gaccggctcg cccccggaga actgaacctc
     3241 gccaagctcc tcgtgggcag cgaaggcacg ctggcgtggt tcgagcgcat cacgctcgat
     3301 ctcgccgaca tcccgaagca caaggcgctg ggggtggcgc acttcccgcg cttctacgac
     3361 gccatggacg ccacgcagca catcgtgaag ctggggccgt gcgcggtcga gctggtggac
     3421 cggacgatga tcgagcttgc gcgcgcgatt cccgcctttg ccgcgaccat cggcgccttc
     3481 gtccgcggcg agccggacgc gatcctcctc gtggaattcg ccggggagga ccgcgacgag
     3541 caggtgcgcg gcgtgaagcg gctcgccgaa ctgatggcgg acctcggctt ccccgggggt
     3601 gtcgtggaga tcctcgatgc cggccggcag aaggagctct gggaggtgcg caaggcgggc
     3661 ctcaacatca tgatgtcgat gaagggcgac ggaaagcccg tgtccttcat cgaggattgc
     3721 gccgttccgc tcgaacacct ggccgaatac acctcgaagc tgacggaggt ctttcgcaag
     3781 cacgggaccg agggcacctt ctatgcccac gcgtccgtgg gttgcctgca cgtgcgcccg
     3841 atcctcaaca tgaagggcga cggcacgatc cgcatgcgcg ccatcgccga ggaggcggcc
     3901 gacctggtgc gccgctacaa gggggcctac tcgggcgagc acggcgacgg gctcgtgcgt
     3961 tcggagtgga tcgcgccctt cttcggcccg cggctcacgg cggcgctcgg ggagatcaag
     4021 ggctggttcg accccagggg gctcatgaac ccgggcaaga tcgtgaaccc gccgaagcag
     4081 gacgaccggt cgctccttcg ctacaagccc ggctaccacg ccgaggcgcc ggcgacggcg
     4141 ctcgactgga gcgcctgggg cgggcttccg ggtgccgtgg agatgtgcaa caacaacggc
     4201 cattgccgca agttcgacgc cggaacgatg tgcccgagct atcgcgtgac gcgcgacgag
     4261 gttcacgtca cgcgggggcg cgccaatacg ctgcgcctcg cgctctcggg gcaactcggc
     4321 gaggatgggc tcgcctcgga tgccgtccgc gacgccttcg acctgtgcgt gtcgtgcaag
     4381 ggctgccgcc gcgagtgccc gaccggcgtc gacatggcgc gcatgaagat cgagttcctg
     4441 aaccacgacc gcgccagaaa cggccacagc gtgcgcgacc gccttgtggg gatgctcccg
     4501 gcctatgcgc cgtgggcatc ccggttcgca ccgctcgtga acgcggccca gcgaatgccc
     4561 ggcgtctccc gtgcgctgca cgcgctcgcg ggattcgatt cgcgccgcga gttgccggcc
     4621 ttcagcgcga ggccctggct ggccgcccag ccgccttacg tggcgagggg gggacgcgag
     4681 gtcgtcctgt tcgcagacac cttcaacaac tggttcgagc ccggaaacct cgcggcggcg
     4741 caacgggtga tttcggctgc ggggttcgaa gtcgtgcccg cgcgcggcga cgatgcacgt
     4801 ccgctttgct gcgggcggac ctacctgtcg gcgggcatgg tcgatcgcgc gaaggccgaa
     4861 tcgcggcgga tgatcgacgc cctggcgccg tggatcgagc gcggcgtccc ggtcgtgggg
     4921 ctggaaccct cgtgccttta ttcgctcaag gacgagtttc tggcgatgca ccccggcgat
     4981 tcgcgcgcga ccgcgctggc ggaactggcc gtgaccttcg agagcttcat tgcccgcgcc
     5041 cttcgcgacg gggccaggat cgcctggcgg gaagggcatg aacccgagtt cctcgtgcac
     5101 ggacactgcc accagaaggc attcggcagc ttcgacgaca ccctggccgc gttgcgcgcg
     5161 attcccggcg cgcgcgtgag cgccgtcgaa tcgagctgtt gcggcatggc gggatcgttc
     5221 ggccacgagc actacgatgt ttcgatggcg atgggagagg catcgctgct gccggccgtg
     5281 cgcgccgcgg caccggcgac ggtcgtcgtg gcagcgggaa cgagctgccg tcaccagatc
     5341 gaccacggcg cgtcgcgcga agcggtgcac ccggcggttg cgctcgcggc cgcgctggcg
     5401 ccgtgaacgc cgtgccctca tcggccccgg tcgtcgaggc cttgcgtgcg gccctggact
     5461 tcacgcctct cggggaaccc gcgggcaacg tcacgctcgg caagggaacg ctggacggcc
     5521 gtccggtgcg cgtggcgctg gtcgagaacc gcatcgccag cgggtccatc ggcaaggcgg
     5581 aagtcgcgaa gctcgtgccc cttttcagca tcgcggcgcg ggagaagtcc gcggtagtcc
     5641 tctatctcga ttccgccggc gcgcgcatct cccaggggct cgaggcgctc ggcgcattcc
     5701 ggaggctgtt tcgcgaggca ctggcggcac ggatcgccgg cgcatccatc gcggtggttc
     5761 tcgggcgcaa ctgctatggc ggggcgagca tgctcgcgca cgtgggggcg aagcgcctgt
     5821 tcagcccggg gacgcaactg gcgatgtcgg ggccgtcgat cctggcgcgg tcggcgggag
     5881 tcgatgcact cgacgagatg ttccgcgcca tggcggatgc cgcgatcggg gccgcggcac
     5941 gggcaaaggc gagcgaagcc aatgccgtct ggacgccggg catggacctc ccggcctggc
     6001 tgcgcgtcgc gctcgcgccg gacgccgggc cctggaacgc tttcctggcc cgtcaccagt
     6061 tgctgcgcac gcgcctcgac aaggggctca ccacgcgcca gccggagaac gtgcggcgca
     6121 aggacctcga gaagctcttt gcacagggct atcgggccat cgaatgcgat ggtgtcatca
     6181 ccggggaggc gacctgcgac ggcgttgccg tcccggttct cgggctcgtc gggccgaatc
     6241 acgtgggcgc ggagcgggca tggcgtttcg ccgacgcggc ctggaggatg gcgaagggcg
     6301 ccccggctcg cgtggaggtc ctgctggatt gcgaatccca tgcggcgcgg ctcgacgacg
     6361 aacgggccgt gctcacggaa ttcatcgtcg acatgggtgt ggctctcgcg gccgtggcgg
     6421 ctcgcggaac gcacgtcgag ctgaccattc tcgaccgcgc gggtggcggc gtctacgtgg
     6481 cgcttgccgc gccggcgacg cacgtttcgg tcgtgttcgg ggccgacatc caggtgctgc
     6541 cgggcgcggc cgtcgcgagc atactgggat cgcaccgcga tgcggtgggc gacatttccg
     6601 actaccgtcg cgccggcgtg gccgacgcgg agcacaagct gggccttcct ccctgagccg
     6661 tcacatgaac gaaaacctct actcgctttt cgaatcgcgc ttccccgcgg accggacgcg
     6721 gccgctgctt atcctcccgg acgggcgcga gatctcctat gggcaggcgg aggagtcctg
     6781 cgcccggttc gcggccttcc tcgccgggcg aggcctgtcg cccggagacc gcgtggcggt
     6841 gcaggtggaa aagtcccccg aggcgctgct cctctacctt gcctgcctgc gcgcgggcct
     6901 cgtgtacctg ccgctcaaca gcgcctacca ggcgggagag gtgggctatt tcctcgacaa
     6961 tgcgcaggcc ggcgccgtca tcgcccagcc acgctcgctg ccgtggctcg agccgctggc
     7021 cgcgaagctc ggcatccggc acgtgttctc gctggacgaa cacgggaaag gcagcttcgt
     7081 cgaggcggcg cacggcgcag gctcgcgctt cgacacggtg gcgcgacgcg ccgacgacgt
     7141 tgcggcgatc ctctacacct cggggaccac cggccgttcc aagggcgcga tgatcacgca
     7201 ccgcaacctc gcgtccaatg cgcaggtcct gcacgcctac tggggattcc ggcccggcga
     7261 catcctcgtg cacatgctgc cgctcttcca tgtgcacggc ctcttcgtcg cctgccattg
     7321 cgtgctcatg aacggcaccg ccatgcgctt ccatgcgaaa ttcgacgccc cgctcgcgat
     7381 cgcggacttc ggccgcagca ccgtcttcat gggcgtgccg accctgtaca cgcgcctgct
     7441 cgccgaggcg ggcctggacc gcaaggcgtg cgcgggcatg cggctcttcg tctcgggttc
     7501 ggcaccgcgg ctcgccgaga cccacgcgca gttcgaggag aggaccggcc atcgcatcct
     7561 ggagcgctac ggcatgaccg agacggggat gctcgtctcc aacccgctgg aaggggagcg
     7621 aaggccgggc acggtgggcc tgccgctgcc cggcacgagc gttcgcgtgg tggacgacac
     7681 cggccggcct tgcaccgccg gggaaatcgg ccatatccag gtgaagggcg acaacgtgct
     7741 gcccggctac tggcgcatgc cggagcggaa caaggaggag ttcacgacgg acggctactt
     7801 caggaccggc gacgtggggt cgttctcgga ggacgggtat ctcgccatcg tcgggcgatc
     7861 caaggacctc atcatcaccg gtggctacaa cgtgtacccg aaggaagtgg agctcgcgat
     7921 cgacgagttg cctgccgtgg cggagtcggc cgtcatcggc gtcccgcacc cggacttcgg
     7981 cgaggcggtc accgcgatcg tcgtgccgcg aaccggtgcg caggcaccga cggaggacga
     8041 ggtcatcgcc tggctcaagt cgcggctcgc gaacttcaag gtcccgaagc gggtcttcgt
     8101 tgcacaggaa ctgccgagga acaccatggg caaggtgcag aagaacgtgc ttcgcgaccg
     8161 ctactcgtcc gtcgggagct gaccatgccc ggcacccgca cgatcctcga caggctcgtt
     8221 ggcacgttcg ccgcggcaac gcgcagccgc agcgagcggc gggcgatccg gctcgccaag
     8281 ctgctgcgca cgctcatctc gcaacgcggc gagtcctcgg gcgcgctcat ggcgcgccgg
     8341 gccgtcgccc tctacaacgg cctggacgag agcggccggc ggctcttctt cgacctgctc
     8401 gcgcgtgatt tctccccgga ccccgacgag gtgctcgccg cggcgcaggc ctaccagcgc
     8461 gagcccgtgc cggccacgct tgcccgcctg cagcgggtgg ccgagccccc gcgccaggag
     8521 gtgtttcgcc gcatgaacat gtcgcccaac gggacggcgg tactcgtggg gatgcgcaag
     8581 gtgctgctcg aggcgctgcc cgccaacccg caattcgcct ccctggacga cgaccttacg
     8641 cacctcttcg gatcctggtt caatcgcgga ttcctggagc ttcgccgcat cgactggggc
     8701 acgccggcct ccatccttga gaagcttatc gcctacgaag ccgtccacga gatccagggt
     8761 ttcccggacc tcaagcgccg gctggcgcac gaccgtcgct gctttgcgtt tttccatccg
     8821 gcgctgccgg gcgagcccct cgtgttcgtc gaggtggcgt tcgtggacga actgccctcc
     8881 gcggtcgcgc ccatcctgtc ccaggaaagc gcggtcggcg acccgcggaa ggcgcggtgc
     8941 gcggtgttct actcgatcac cagttgccag ccggggctgc gcggcgtctc ctttggcaac
     9001 ttcctcatca agcaggttgc gcaggatctt cacgaggagc tgccgaacct gcgcgtcttc
     9061 gccacgcttt cgcccatgcc gggctttcgc gcgtggctgc gccgccagcc tgacgatcgc
     9121 gtgctggcgg cggcgccacc catccgcttc gccgcgcagg ccgatcccgc gagggcgccg
     9181 ggccaggaac ttcgcgaacc cctgcaggcg ctcgccgcgg tctatctcgc gaaacaatgg
     9241 gaccagggcc acgtgctcga tccggctgca cgcttccacc tcggcaatgg cgcgcgcctc
     9301 gagcgcatca actggcttgc ggatacctcg gcgagagggc tgcaccagtc gctcggcctg
     9361 atggccaatt tcgtctatgc cctcgacgac gtggagcgca accacgaaag ttacgtgaac
     9421 cgccacgtcg tcgcctgttc gccggccgtg gagcgcctgg tgcggttcgc cgaaggagac
     9481 gtgaaggggg cgcccagtgc gtgactttcc cgcacggacg ttgaaaacgc ccaccgtcgc
     9541 ccctatttga atttcatgaa ggtctttcgc ctctcctgcg cccacggtca cgacttcgag
     9601 ggctggttcg cctcctcgga ggacttcgat cgccagcagg cgggcggcga gatcagttgc
     9661 ccgctgtgcg agaaccgcag cgtggcgaag cttccatccg ccccttacgt gaacaccggc
     9721 gcgcgcggca cgcaaacgat gtccgtggcc gctccggcca ccccggcgac tccggacctt
     9781 tcagtcgcac tggcggcgct cagggcctat gtgtcggcca acaccgaaaa tgtcggccgc
     9841 cagttccccg aggttgcgcg caggatgcat tacggggagg aatcgcaacg cggcatccgc
     9901 ggccgcgtga cgaccgagga agccgtggcg ctgcgcgaag agggcatcga ggccatcccg
     9961 ctgccgcccg gcctcgcgct ggacgagaag attcactgag ggacggcgcg gccgcgccgg
    10021 tcagaacgcc gaaatcccgg tctgcgcgcg cccgaggatg agcgcgtgca cgtcgtgcgt
    10081 gccttcgtag gtgttcaccg cctcgaggtt cagcacgtgg cggatcacgt ggtactcgtc
    10141 cgcgatcccg ttgccgccgt gcatgtcgcg cgccgtccgc gcgatgtcca gcgccttgcc
    10201 gcaggaattg cgcttgacca gcgagaccat ctccggcgtg gcgcggccct cgtcgagcag
    10261 ccgcgccacg cgcagcaccg cctggaggcc gatcgcgatt tccgtctgca tgtcggcaag
    10321 ctttttctgg atgagctggt tcgcggcgag aggcttgccg aactggtggc ggtctagggt
    10381 gtactggcgc gcggcgtgcc agcagaattc cgccgccccc agcgcccccc aggaaatccc
    10441 gaagcgcgcc ttgttgaggc agccgaacgg ccccttcagg cccttcacgt tgggcaggta
    10501 gttgtcgtcg gggacgaaca cgtcctccat caccacctcg ccggtgatcg acgcacgcag
    10561 gctgaacttg ccttcgatct tgggcgccga gagccccttc atgcccttct cgaggagata
    10621 gccgcggatg tcgccggcgt cgtccttggc ccagacgacg aagaggtcgg cgatgggcga
    10681 attcgtgatc cacatcttgg cgccggacac gcgccagccg ccatccacct tgcgcgcgcg
    10741 cgtgaccatg ccgcccgggt cggacccgtg atcgggctcg gtgaggccga agcagccgac
    10801 gagctcgccg gtggcgagcc gcggcagcca cttctgccgc tgcgcttcgg tgccgtaggc
    10861 gtggatcggg tgcatgacga gcgaggactg cacgctcatc gtggagcggt agccggaatc
    10921 gacgcgctcg acctcgcgcg cgatgaggcc gtaacagaca ttgttcacgc ccgcgcagcc
    10981 atagccctcg atcgtggagc cgaggaagcc cagttcaccc atctcgttga ggatgccgcg
    11041 gtcgaagcgc tcgtgccggt gggcctcgag gattcgcggc ttgagctttt cctggctgta
    11101 ggcgagggcg ctctcgcgga ccatgcgctc ctcctcggtg agctgggcat cgagaaggaa
    11161 gggatcgtcc cactggaaaa tgggcttcgg gtcgctgggt ttcatgggct ctccggaaat
    11221 tccgcccatt ctaccgccgg ccaccggtag aatcgcccgc catggctacg caaatccgac
    11281 ggctctctgc agaagacctc tggcggctcg cgcggcccgc gcagcccacg ctctcacccg
    11341 atggcgcaca ggcgtgcgta tcggtcacta cctacga
//
LOCUS       DASUEV010000157         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_847754, whole genome shotgun sequence.
ACCESSION   DASUEV010000157 DASUEV010000000
VERSION     DASUEV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847754"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="glyS"
                     /locus_tag="VFV55_05185"
     CDS             complement(<1..1077)
                     /gene="glyS"
                     /locus_tag="VFV55_05185"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX4943725.1"
                     /translation="MSATLLVELFTEELPPKALNRLGESFASGVEAGLRKRGFLGEAS
                     AASGFATPRRLAVSITAVRAATEPSMVEVKLMPVAVGLGADGQPTPALRKKLASSGLE
                     GIDAARLKRRVDGKAETLFADAVAPSVALAEGLQAALEEAIAGLPIPKVMSYQLADGV
                     TTVQFVRPAHGLVALHGADIVKVAVLGLAAGRITHGHRFQGARDITLANAEEYAIRLS
                     AEGGVLAGFAGRRAAILAALKAAATQQASTLGPEGEYTALLDEVTGLVEMPTVYVGRF
                     EQEFLAVPQECLILTMRQNQKYFPLFDAGGKLTEKFLVVSNLRPADPSRIIQGNERVV
                     RPRLADARFFFETDRKTPLAERVAQ"
     gene            complement(1074..1976)
                     /gene="glyQ"
                     /locus_tag="VFV55_05190"
     CDS             complement(1074..1976)
                     /gene="glyQ"
                     /locus_tag="VFV55_05190"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX4943726.1"
                     /translation="MITFQDIILRLQAYWGSRGCALLQPYDMEVGAGTSHTATFLRSL
                     GPEPWRAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPSPPDILELYLGSLEALGF
                     DLQENDVRFVEDDWENPTLGAWGLGWEVWMNGMEITQFTYFQQVGGLDCKPITGEITY
                     GLERLAMYLQGVENVFDLVWTEREGGGRTRRLTYRDVYHQNEVEQSTYNFEQSDTEAL
                     FRHFGDHEKGARHLMAERLALPAYEQVLKAAHAFNLLDARGAISVTERAAYIGRIRNL
                     AKAVAQSYFESRERLGFPMLGATA"
     gene            2116..>3160
                     /locus_tag="VFV55_05195"
     CDS             2116..>3160
                     /locus_tag="VFV55_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4943727.1"
                     /translation="MAAVPPGFNPDKDGTSLYDILCEAATHHQAGRLAQADALYRTVL
                     RAHPDNRDAHHNLGVLAMQRGLGVEKALVHFRTAWEVDPSHAQHGLSYLRALVLAGDL
                     EQARLVRADGFRRGFAWPSVETLAAGLTAGKGARPPADGAAAPGRDDAEALAKAFASD
                     DFPRFESLARSLAERYPGHGLGWKALGVACLRLGRSEEAIESLRKAARLLPDDPETHG
                     HLGNLLLETGLPGEAEVALRRVVSLQPRLARTHVRLGDALCALHRFPEAEAEFRAALD
                     LDRGSAQAHCGLGRTLASQERLEDAKASYRRALTIEPGHPEAYAGIGAILMGQQRMAE
                     AEALCREALARDPL"
BASE COUNT          487 a         1054 c         1100 g          519 t
ORIGIN      
        1 ttgcgcaacg cgctcggcga gcggggtctt cctgtccgtc tcgaagaaga agcgcgcgtc
       61 ggccaggcgc gggcgcacca cgcgctcgtt gccctgaatg atgcgcgagg gatccgccgg
      121 gcgcaggttc gagacgacga gaaacttttc cgtgagcttc ccgccggcgt cgaacagcgg
      181 gaagtacttc tggttctggc gcatcgtcag gatgaggcat tcctgcggca cggccaggaa
      241 ctcctgctcg aatcggccga cgtagaccgt cggcatctcc acgagacccg tcacctcgtc
      301 gaggagcgcc gtgtattcgc cttccgggcc gagcgtcgat gcctgctgcg tggcggcggc
      361 cttcagtgcc gccaggatcg ccgcgcggcg cccggcaaaa ccggccagta cgccgccttc
      421 ggccgaaagc cggattgcgt actcctccgc gttcgccagc gtgatgtcgc gtgcgccctg
      481 gaagcggtgg ccgtgcgtga tccgacccgc tgcaagtccg agcaccgcga ctttcacgat
      541 gtcggctcca tgaagcgcga ccagcccgtg cgcggggcgg acgaactgca cggtggtgac
      601 gccgtcggcg agctggtaac tcatcacctt cgggatgggc agccccgcga tggcctcctc
      661 gagcgccgcc tggagccctt cggccagagc aacggagggc gccacggcgt cggcaaagag
      721 ggtctcggcc ttgccgtcga cgcggcgctt gagtcttgcg gcgtcgattc cctccagccc
      781 ggacgacgcc agtttcttgc gcagcgccgg cgtgggctgg ccatccgccc cgagtcccac
      841 cgccacgggc atgagcttca cttccaccat cgagggctcg gtggccgcgc gaacagcggt
      901 aatggaaacg gccagccggc gcggtgtggc aaacccgctg gccgcggagg cctctcccag
      961 gaatccgcgt ttgcgcaacc ccgcctcgac acccgatgcg aacgactctc ccagccggtt
     1021 gagcgccttg ggcgggagtt cctcggtgaa aagctcgacc aggagtgtgg cgctcacgcc
     1081 gtggctccga gcatcgggaa gccgagtcgc tcgcgggact cgaagtagct ctgcgccacc
     1141 gccttcgcga gattgcggat gcggccgatg taggccgcgc gctccgtgac cgagatggcg
     1201 ccgcgcgcat cgagcaggtt gaaggcgtgc gcggccttga gcacctgctc gtacgcgggg
     1261 agcgccagcc gttccgccat caggtgcctg gcgcccttct cgtggtcgcc gaagtgccgg
     1321 aagagcgcct cggtatccga ctgctcgaag ttgtaggtcg attgctcgac ctcgttctgg
     1381 tgatagacgt cgcggtaggt gagcctgcgc gtgcgccctc ccccttcgcg ctcggtccat
     1441 accaggtcga agacgttctc cacgccctgc aggtacatcg cgaggcgctc caggccatag
     1501 gtgatctcgc ccgtgatcgg cttgcagtcg aggccgccca cctgctggaa gtaggtgaac
     1561 tgggtgatct ccatgccgtt catccagact tcccaaccca ggccccacgc gccaagcgtc
     1621 gggttctccc agtcgtcctc gacgaagcgc acgtcgttct cctgcaggtc gaagcccagg
     1681 gcttcgagcg agcccaggta gagctcgagg atgtccggcg gcgagggctt cagcaccacc
     1741 tggtactggt agtagtgctg caggcggttg gggttgtcgc cgtagcgccc gtccttcggc
     1801 cggcgcgaag gctggacata ggctgcccgc cacggctccg gacccagcga gcgcaggaag
     1861 gtggcggtgt gggacgtgcc cgcgcccact tccatgtcgt agggctggag cagggcacag
     1921 ccgcggctgc cccaataggc ctggaggcga aggatgatgt cctggaaggt gatcatggca
     1981 ggcggcggag aatcggtcat tttacggggt cggcccgcga agcgggaaat gcgttgatat
     2041 actttgcacc gcccttgcgc ggcccggttc gcctgcacgc gttttcggca ccgcgacccc
     2101 taccggcgga tactcatggc cgcagtccca ccaggcttca atccggacaa agacggcacg
     2161 tcgctctacg acattctttg cgaggccgcg acgcatcacc aggccggtcg gctcgcgcag
     2221 gcggatgcgt tgtacaggac tgtcctgcgc gcgcaccccg acaaccgcga cgcccatcac
     2281 aacctcggcg tgctcgccat gcagcgcggg ctcggcgtcg aaaaggcgct ggtgcatttc
     2341 aggacggcct gggaggtcga tccttcgcac gctcagcacg gcctgagcta ccttcgggcg
     2401 ctcgttctcg cgggcgacct cgagcaggcg cgtctcgtcc gtgcggatgg attccgccgg
     2461 ggattcgcgt ggccttccgt ggagacgctc gcggccggcc tgaccgcggg gaaaggcgca
     2521 aggcccccgg ccgatggcgc cgcggctccc ggccgggacg atgcggaagc cctcgcgaag
     2581 gcatttgcgt cggacgactt tccccgtttc gaatcgcttg cgcgttcgct ggcggaacga
     2641 tacccgggac acgggctggg ctggaaggca ctgggcgtag cctgcctgcg cctgggccga
     2701 tcggaggagg cgatcgagtc gctgcgaaag gcggctcggc tcctgccgga cgacccggaa
     2761 acgcacggcc acctgggaaa ccttctgctc gaaacgggac tgccgggcga ggcggaagtt
     2821 gctttgcgtc gggtggtgag ccttcagccc cggctggcga ggacgcatgt ccgcctgggc
     2881 gatgcgttgt gcgcgctgca tcggttcccc gaggccgagg ccgagtttcg cgcggcattg
     2941 gaccttgatc ggggctccgc acaggcccat tgcggactcg gtcgcacact ggcgtcacag
     3001 gaacggctgg aggatgccaa ggcaagctac cggcgggccc tgacgatcga gccggggcac
     3061 cccgaggcgt acgcgggaat cggtgcaatc ctcatggggc agcagcggat ggcggaagcg
     3121 gaggcgctgt gccgggaagc gctggcccgc gacccgttgc
//
LOCUS       DASUEV010000158         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_261126, whole genome shotgun sequence.
ACCESSION   DASUEV010000158 DASUEV010000000
VERSION     DASUEV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261126"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFV55_05200"
     CDS             complement(<1..993)
                     /locus_tag="VFV55_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4943728.1"
                     /translation="MRYHVRALRNGGELAALTFDADTEAAARNQATSQGLSVLSVRAE
                     RRSLPRPFRARSKFPLLLFTQEFISLLDAGLGATEALEALAGKEGAGGALRAVLGHVS
                     AGRSLSYAFEQGGADFPPLFVATIRASEKTGDMKEALSRYAAYFGQVESLRKKVVNAL
                     VYPAMLAVTGGLVTAFLMFYVVPRFSRIYEDIGGNLPFLSQILMKWGQLLSNHATALF
                     LSLAALALIVVRVGSRPQVRERLMRAAWRAPRIGEALQVYHLARFYRTMGMLLRSGMP
                     LVGALGMARGLLAPAMRPSLDAASRSISEGIPASTALTECGLTTPVGLSMLRVGE"
     gene            complement(999..1427)
                     /gene="gspG"
                     /locus_tag="VFV55_05205"
     CDS             complement(999..1427)
                     /gene="gspG"
                     /locus_tag="VFV55_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX4943729.1"
                     /translation="MNGRTPRRPAARGFTLLELMVVMVIIGLLAAYVAPRYFSQVGKS
                     ETKLARAQIDAFEKALEQYRADTSHFPTTEQGLAALMTRPTGENRWDGPYLKRQIPPD
                     PWGRPYQYKSPGEHGEIDIFSLGRDGRPGGSGDSADIGNW"
     gene            complement(1458..2018)
                     /locus_tag="VFV55_05210"
     CDS             complement(1458..2018)
                     /locus_tag="VFV55_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4943730.1"
                     /translation="MEPNLKNPDNDGPYILVVDDDETFLSVLARSLERRGYKVRTAPD
                     HECAISIAQARLPEFAVVDLKLARSSGLRLVKQLREMAPNTRIVILTGYGSIATTIEA
                     IKLGAVYYLTKPATADDVIAAFNREEASEEIPAAPIPPSFNRLEWEHVNRVLAETNGN
                     ISASARILGWHRRTLQRKLGKRPVER"
     gene            complement(2035..2403)
                     /locus_tag="VFV55_05215"
     CDS             complement(2035..2403)
                     /locus_tag="VFV55_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943731.1"
                     /translation="MKLSWPEESADPPDIVIELSLFNALAGLMKDFIRDDPHVAHVSA
                     AWDAKDVVIRLGGAKDSEADDDADEKADMPFRGRRRERLAVVAAIVDRHGGTVVLEPG
                     GGRRITIRLPHADHGPDRRE"
BASE COUNT          384 a          809 c          835 g          393 t
ORIGIN      
        1 ctccccgacg cgcagcatgc tcaggcccac cggcgtagtc agtccgcact ccgtgagcgc
       61 ggtggaggcc ggaatgcctt ccgagatgct tcggctggcc gcatcgaggg acgggcgcat
      121 ggcgggggcg agcaggccgc gcgccatgcc cagcgcaccc acgagcggca tcccgcttct
      181 caggagcatg cccatcgtcc ggtagaaccg cgccaggtgg tagacctgga gcgcctcgcc
      241 gatgcgcggt gcacgccagg cggcccgcat caggcgctcg cggacctgcg gccgcgatcc
      301 cacccggacc acgatcaacg ccagcgcggc cagcgacagg aagagcgccg ttgcgtggtt
      361 ggacagaagc tggccccact tcatgaggat ctgcgacagg aacggcaggt tgccgccgat
      421 gtcctcgtag atgcgactga aacgcggcac gacgtagaac atcaggaacg cagtgacgag
      481 accgcccgtg accgcgagca tcgccggata caccagggca ttgaccactt tcttgcgcag
      541 gctttccacc tggccgaagt acgcggcgta gcgcgacagg gcctctttca tgtcgcctgt
      601 cttctcgctg gcgcggatgg tggcgacgaa gagcggcggg aaatccgccc ccccttgctc
      661 gaaggcgtac gaaaggctgc ggccggccga cacgtggccc aggacggcgc gcagggcgcc
      721 acccgcgccc tccttgccgg ccagcgcctc gagcgcttcg gtcgcaccga ggccggcgtc
      781 aagcagcgag atgaattcct gcgtgaagag gaggagaggg aacttcgagc gcgcgcggaa
      841 cggtcgcggc agcgaccggc gttcggcgcg caccgacagc acgctcagcc cctggctggt
      901 cgcctggttt cgcgcggccg cctccgtatc ggcgtcaaag gtgagcgccg cgagttcgcc
      961 gccgttgcgc agggcccgga cgtgatagcg cacgatgcct accagttgcc gatgtcggcc
     1021 gagtcgccgg agccgccggg ccgcccgtcg cggccgagcg aaaagatgtc gatctcgccg
     1081 tgctcgccgg gtgacttgta ctggtagggg cggccccacg ggtcgggcgg aatctggcgc
     1141 ttgaggtagg gcccgtccca gcgattttcg ccggtcggac gcgtcatgag ggcggccagc
     1201 ccctgctcgg tggtcgggaa gtgggaagtg tcggcccggt attgttcgag cgccttctcg
     1261 aaggcgtcga tctgggcgcg ggccagcttg gtctccgact tgccgacctg cgaaaagtag
     1321 cgcggtgcga cataggccgc aaggaggccg atgatcacca tcacgaccat cagctcgaga
     1381 agcgtgaatc cccgcgcggc gggccggcgc ggcgttctac cgttcatcga ttgccgttcc
     1441 cgcccggaag ggccgcgtca acgctccacc gggcgcttgc ccagcttccg ctgcaaggtg
     1501 cgccggtgcc agcccaggat gcgggccgat gcggagatgt tgccgttcgt ttcggcgagg
     1561 acgcgattca cgtgctccca ctcgagacgg ttgaacgacg gcgggatggg cgccgccggg
     1621 atctcctccg aggcctcttc gcggttgaac gcggcaatga cgtcgtcggc cgtggccggc
     1681 ttcgtgaggt agtagaccgc gcccagcttg atcgcctcga tcgtggtggc gatgctgcca
     1741 tagccggtga ggatcacgat cctcgtattc ggggccatct cgcgaagctg cttcaccagc
     1801 cgcagtccgg agcttcgtgc gagcttgagg tccaccacgg cgaattcggg caggcgggcc
     1861 tgcgcgatcg agatggcgca ctcatggtcg ggcgccgtgc gcaccttgta gccccgccgt
     1921 tcgagcgagc gcgccaggac ggacaggaac gtctcgtcgt cgtccactac caggatataa
     1981 ggcccgtcgt tgtcgggatt cttcaggttc ggttccattt agcgcctccc ttgcctattc
     2041 gcgccggtcc ggtccgtgat cggcgtgcgg aaggcgaatc gtgattcgcc gcccgccgcc
     2101 cggttcgagg acgaccgtac caccgtgccg atcgacaatg gcggcaacca ccgcaaggcg
     2161 ttcgcgccgc cggccgcgga agggcatgtc cgccttctcg tcagcatcgt cgtctgcctc
     2221 gctgtccttc gcgccgccga ggcggatcac gacgtctttg gcatcccatg cggcgctcac
     2281 atgcgcgaca tgcggatcgt cgcggatgaa atccttcatc agcccggcca gcgcgttgaa
     2341 gagcgacagc tcgatcacga tgtcgggtgg atccgcggat tcctccggcc aggaaagctt
     2401 cacgtgcccg gcgggatagg a
//
LOCUS       DASUEV010000159         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_848049, whole genome shotgun sequence.
ACCESSION   DASUEV010000159 DASUEV010000000
VERSION     DASUEV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848049"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VFV55_05220"
     CDS             complement(<1..116)
                     /locus_tag="VFV55_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4943732.1"
                     /translation="MSRTILVMAAGTGGHVFPGLAIAKELARRGWKVVWMGTP"
     gene            complement(113..1264)
                     /gene="ftsW"
                     /locus_tag="VFV55_05225"
     CDS             complement(113..1264)
                     /gene="ftsW"
                     /locus_tag="VFV55_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX4943733.1"
                     /translation="MLYNPARRDTAIDQGLVWSAVLLASFGLVMVYSASIAMAEAERF
                     TGFRAHYFLARHGIYLVAGLAAGAMAFRVPVRYWQAAAPWIFMAGVGLLVAALIPGVG
                     REVNGARRWIALGPATFQPSEVMKLLVVLYAADYTVRKAAFMDDLRKGFLPMFAVMFL
                     VAGLLLREPDFGALVVVTAIAMAILFLGGLNWRIFVVLVAGLAAAFVLLIVFSPYRLQ
                     RILGFMDPWSDAYGKGYQLSHSLIAFGRGEWLGVGLGASVEKLFYLPEAHTDFLLAVI
                     AEELGLVGVAAVIALFLFLVVRAFAVGRQAASLERYYAALVAQGIGVWLAAQAFINMG
                     VNMGLLPTKGLTLPFLSFGGTGIVVNCVAVAIVLRIDFENRYLMRGGSL"
     gene            complement(1264..2652)
                     /gene="murD"
                     /locus_tag="VFV55_05230"
     CDS             complement(1264..2652)
                     /gene="murD"
                     /locus_tag="VFV55_05230"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX4943734.1"
                     /translation="MRNGDWAGKTVLVLGLGDTGLSCVRWLARKGARLRGADTRQAPP
                     GLGAVREAVPGIRVDLGPFGSASLAGVDAVAASPGIALREPVLREAAARGIEIVGDVE
                     IFAREVARSAPGARVIGITGTNGKSTVTALVGAMSRAAGRRTVVAGNIGLPVLDALRD
                     AEDRGHPEYYVIELSSYQLETTASLALASAAMLNLSQDHLDRYDSMQAYADAKARIFR
                     HGGTRVLNRDDPWSIGMKGGRTHTFGLDAPRNDEEWGIANGRLVQGREAIVALEELPM
                     AGLHNAANALAAHALCRSAGLGDGAGPGEDSLARAIREFRGLPHRVERVAAARGVVFY
                     DDSKGTNVGATVAALEGMRETSVLIAGGDGKGQDFAPLVPAVRQKARAVVLIGRDAPA
                     VEAALAPAGVPCQRAATMAEAVEVAFGLAKAGDAVLLSPACASLDMFANYKQRGEVFA
                     TAARALAKRMEG"
     gene            complement(2665..3750)
                     /gene="mraY"
                     /locus_tag="VFV55_05235"
     CDS             complement(2665..3750)
                     /gene="mraY"
                     /locus_tag="VFV55_05235"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX4943735.1"
                     /translation="MLLELAQWLSTDIRTFNVFTYITLRVVLAALTALAISFVIGPAM
                     IRRLAAYKIGQSVRDDGPQTHLVKTGTPTMGGALILVSVAVTTLLWADLQNRYVWICL
                     ATTLGFGAIGWVDDWRKVVQRNPKGLTARSKFFWQSMIAVAAVATLAWSARLPQQTEL
                     IVPFFKTVAIPLGAIGFVILGYFVVVGTSNAVNLTDGLDGLAIMPTVMIASALAVFAY
                     VTGHAVFSKYLGFPFIPGAGELAVFCAAIAGAGLAFLWFNAYPAEVFMGDVGALALGA
                     ALGMVAIVVRQEIVLFMMGGVFVVETLSVVIQVASFKLTGKRVFRMAPLHHHYELKGW
                     KENQVVVRFWIITMMLVLFGLSTLKLR"
     gene            complement(3740..>4873)
                     /gene="murF"
                     /locus_tag="VFV55_05240"
     CDS             complement(3740..>4873)
                     /gene="murF"
                     /locus_tag="VFV55_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX4943736.1"
                     /translation="KDTRAALGRLGASWRARFSLPVVALTGSNGKTTVKEMLAAILAA
                     HCGGRDDVLATEGNLNNDIGLPLMLLRLRDRHRFAVFEMGMNHAGEIDYLSRLASPSV
                     ALVNNAHRAHIGMLGTLEAIAQAKGEIYAGLRAGGIALVNEDDPFAGYWKRLATGRRV
                     VTFGTADSADVRGQIGDGQVGDSPQLRFITPLDAFIVTLQVPGEHNARNAIAAGAAAH
                     ALDIPSRAIQAGLAAFAGTRGRLERRSGDGGSALLDDTYNANPDSMKAAIRVLAAAPG
                     RRVFVMGDMGELGEGAALLHAEIGAFAKDAGVERLLALGPESVHAVRTFGDGATHFDT
                     LEALVSEARAQAQPGVTILVKGSRFMRMERVVDRLAAKDSHAA"
BASE COUNT          813 a         1749 c         1641 g          670 t
ORIGIN      
        1 ggcgtgccca tccacacgac cttccagccg cgccgcgcga gctccttcgc gatcgcgagg
       61 ccggggaaga cgtgcccgcc cgtgcccgcg gccatgacga ggatcgtgcg gctcacagcg
      121 agcctccccg catcaggtag cggttctcga agtcgatgcg cagcacgatc gccaccgcga
      181 cgcagttcac cacgatgccg gtgccgccga aggagaggaa agggagcgtg aggcccttgg
      241 tgggcagcag ccccatgttc acgcccatgt tgatgaaggc ctgcgccgcc agccacacgc
      301 cgatgccctg cgcgaccagc gcggcgtagt agcgctccag gctcgcggcc tggcggccca
      361 cggcgaaggc gcgcaccacg aggaagagga agagcgcgat caccgccgcc acccccacga
      421 ggcccagttc ctccgcgatc accgcgagca ggaagtcggt gtgcgcttcg ggcaggtaga
      481 agagcttctc caccgaggca cccagtccca ctcccagcca ttcgccgcgc ccgaaggcga
      541 tgagcgaatg cgagagctgg tagcccttgc cgtaggcgtc cgaccagggg tccatgaagc
      601 cgaggatgcg ctgcagccgg tagggggaga agacgatgag cagcacgaac gcggccgcga
      661 gccccgccac gaggacgacg aagatgcgcc agttgagccc gcccaggaag aggatcgcca
      721 tcgcgatggc ggtcacgacc accagcgcgc cgaagtccgg ttcgcgcagc agcagcccgg
      781 cgacgaggaa catcaccgcg aacatgggca ggaatccctt cctcaggtcg tccatgaacg
      841 cggccttgcg cacggtgtag tcggccgcgt agagcaccac gaggagcttc atgacctccg
      901 agggctggaa cgtggcgggg ccaagggcga tccaccggcg cgcgccgttc acctcgcggc
      961 cgacgcccgg gatcagcgcg gccacgagca gccccacgcc cgccatgaat atccagggtg
     1021 ccgccgcctg ccagtagcgc accggcaccc ggaatgccat cgcgcccgcg gcgaggccgg
     1081 ccacgaggta gattccgtgc cgcgccagga agtagtgggc gcgaaagccc gtgaaacgct
     1141 ccgcctcggc catggcgatc gaggctgaat agaccatcac cagcccgaac gacgcgagca
     1201 gcaccgcact ccagacgaga ccctggtcga tggcggtgtc gcggcgtgcg gggttgtaga
     1261 gcatcagccc tccatccgct ttgccagcgc tcgcgcggcg gttgcgaaga cctcgccgcg
     1321 ctgcttgtaa ttggcgaaca tgtcgaggct cgcgcacgcg ggcgacagga gaacggcatc
     1381 gcccgccttg gcaagcccga acgccacctc gaccgcttcc gccatcgtcg ccgcgcgctg
     1441 gcacggcacg cccgcggggg ccagagccgc ttccacggcc ggcgcgtcgc gcccgatgag
     1501 cacgaccgcc ctcgcctttt gcctgaccgc gggaacgagc ggcgcgaagt cctggccctt
     1561 gccgtcgccg ccggcgatga gcacggacgt ctcccgcatt ccctccaggg cggcgaccgt
     1621 ggcaccgaca ttcgttccct tggagtcgtc gtagaaaacg acgccgcgcg ccgccgccac
     1681 gcgctcgacg cgatgcggaa ggccgcggaa ctcgcgaatg gcacgcgcga gcgaatcctc
     1741 gcccggcccg gcgccgtcgc ccagcccggc gctgcggcac agcgcgtgcg cggccagcgc
     1801 gttggcggcg ttgtgcagcc ccgccatcgg cagttcttcg agcgccacga tggcctcgcg
     1861 gccttgcacc agccgtccgt tggcgatccc ccactcttca tcgttgcgtg gcgcgtccag
     1921 gccgaaggtg tgcgttcggc cgcccttcat gccgatgctc cacgggtcgt cccggttgag
     1981 cacgcgcgtc ccgccgtgcc ggaaaatgcg cgccttggcg tccgcgtaag cctgcatcga
     2041 gtcgtaacgg tcgaggtgat cctgagagag gttgagcatc gccgccgacg ccagggcgag
     2101 gctcgcggtc gtctccagct ggtagctcga cagctcgatc acgtagtact cggggtgacc
     2161 cctgtcttcg gcatcgcgca gcgcgtccag caccggcagg ccgatgttgc cggccaccac
     2221 cgtgcgccgc cccgcggcgc gcgacatcgc cccgacgagc gccgtcacgg tgctcttgcc
     2281 gttggtgccg gtgattccga tcacgcgtgc tcctggggcc gagcgcgcca cttcgcgcgc
     2341 gaagatctcg acgtcgccga cgatctcgat gccgcgtgcg gcggcttcgc gcaggaccgg
     2401 ctcgcgaagc gcaatgcccg gactcgcagc gaccgcgtcc acgcctgcca ggctggcgga
     2461 tccgaacggg ccgaggtcga cgcggatacc agggaccgcc tcgcggaccg ctcccaggcc
     2521 cggaggcgct tgccgcgtat cggccccgcg cagccgcgcg cccttccgag cgagccaacg
     2581 aacgcacgaa agccccgtgt caccgagccc cagaacgaga acggtcttgc ctgcccagtc
     2641 gccatttcgc atctcctctc gcgcctaacg cagcttcagg gtcgacagcc cgaagagaac
     2701 gagcatcatc gtgatgatcc agaagcggac cacgacctgg ttttccttcc accccttgag
     2761 ctcgtagtgg tggtgcagcg gcgccatgcg gaagacgcgc ttgccggtga gcttgaagga
     2821 ggccacctgg atcaccacgg acagcgtctc caccacgaac acgccgccca tcatgaaaag
     2881 cacgatctcc tgccggacca cgattgccac catgccgagc gcggcgccca gcgccagcgc
     2941 gcccacgtcg cccatgaaga cctccgccgg gtaggcgttg aaccacagga acgccagccc
     3001 ggcgccggcg atggcggcgc agaagaccgc gagctcgccc gcgcccggga tgaacgggaa
     3061 gcccaggtac ttcgagaaca ccgcgtgccc ggtcacgtag gcgaacacgg cgagcgccga
     3121 ggcgatcatc accgtgggca tgatcgccag cccgtccagg ccgtcggtga ggttcaccgc
     3181 gttcgaggtg cccacgacga cgaaatagcc caggatcacg aagccgatcg ccccgagggg
     3241 gatggccacc gtcttgaaga acgggacgat gagctcggtc tgctgcggca gcctggcgct
     3301 ccacgcgagg gtggccacgg ccgcgaccgc gatcatcgac tgccagaaga acttcgagcg
     3361 cgcggtgagt cccttgggat tgcgctgcac gaccttgcgc cagtcgtcga cccagccgat
     3421 ggcgccgaag ccgagcgtgg tggcgaggca gatccacacg tagcggttct gcaggtccgc
     3481 ccacagaagg gtagtcacgg ccaccgacac caggatgagc gccccgccca tggtcggcgt
     3541 ccccgtcttc acgaggtgcg tctgcggccc gtcgtcgcgc acggactgcc cgatcttgta
     3601 ggcggccagc cgccggatca tggccgggcc gatgacgaag gaaatggcga gcgcggtaag
     3661 tgccgcgagc accacgcgca gcgtgatgta ggtgaagacg ttgaacgtcc ggatatcggt
     3721 cgagagccac tgggcgagtt caagcagcat ggctgtcctt cgcggcgagc cggtccacca
     3781 ctcgctccat gcgcatgaac cgcgacccct tcaccaggat cgtgacgccc ggctgcgcct
     3841 gcgcgcgcgc ttcggagacg agagcctcga gcgtgtcgaa gtgcgtggcg ccatcgccga
     3901 acgtccgcac cgcgtgcacg ctctccgggc cgagggcgag cagccgctcg acgccggcgt
     3961 ctttcgcgaa cgcgccgatc tccgcgtgaa gtagggccgc gccctcgccc agctcgccca
     4021 tgtcgcccat cacgaagaca cgccggccgg gcgcagcggc gagcacgcgg atcgcggcct
     4081 tcatcgaatc ggggttggcg ttgtaggtgt cgtccagcag ggccgatccg ccgtcgccgc
     4141 ttctgcgctc caggcggccc cgggtgccgg cgaaggccgc gagccccgcc tggatggcac
     4201 gggaaggaat gtcgagcgcg tgggccgcgg cgcccgccgc gatcgcgttg cgcgcgttgt
     4261 gctcgcccgg cacctgcagc gtcacgataa aagcgtcgag cggcgtgatg aagcgcagtt
     4321 ggggactgtc cccaacttga ccgtccccaa tttgaccgcg cacgtccgcc gaatccgccg
     4381 tgccgaaggt gaccacgcgg cgccccgtgg cgagccgctt ccagtaaccg gcgaacgggt
     4441 cgtcctcgtt cacgagcgcg atgcctccgg cgcgcaggcc cgcgtagatc tcgcccttgg
     4501 cctgcgcgat ggcttccagc gttccgagca tcccgatgtg cgcgcggtgg gcattgttca
     4561 ccagcgccac gctcggggag gcaaggcgcg agaggtaatc gatctcgccc gcatggttca
     4621 tgcccatctc gaagaccgcg aagcgatgcc ggtcgcgcag cctgaggagc atcagcggca
     4681 ggccgatgtc gttgttgagg ttgccttccg tggcgagcac gtcgtcacgc ccgccgcaat
     4741 gtgcggcgag gatcgcggcg agcatctcct tcaccgtcgt cttgccgttg ctgccggtca
     4801 gcgcgaccac ggggagcgaa aagcgcgcgc gccaggaggc gcccagccgg cccagggccg
     4861 cccgcgtgtc ctt
//
LOCUS       DASUEV010000160         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_46464, whole genome shotgun sequence.
ACCESSION   DASUEV010000160 DASUEV010000000
VERSION     DASUEV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46464"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..>2355)
                     /locus_tag="VFV55_05245"
     CDS             complement(6..>2355)
                     /locus_tag="VFV55_05245"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX4943737.1"
                     /translation="DHDWLIATALATRDQIVERWMEATRKTYRDGRKRVYYFSLEFLI
                     GRLLLDSLGNLGLVATAREALADLGIDLDRLRKLEPDAALGNGGLGRLAACFMESMST
                     LGIPAHGYGIRYDHGIFRQAIKNGWQIELPENWLSSGNPWEFERPEVSYTIGFGGAVD
                     AVPNVDGTTRSVWRPADSVNAVAYDTPIAGWRGAHVNTLRLWSARSNHPIVLEDFNRG
                     DHVGALADRVRLEAISRVLYPSDDTPAGHELRLRQEIFFASASLQDLLRRHKQQHGEI
                     SSLPDHVAIQLNDTHPAIAVAELMRLLVDEHGMPWNHAWRICTSVFSYTNHTLLPEAL
                     EKWSVPLMEGLLPRHMQIIFLINARHLDSLRAAGRNDTRLLASLSLIEENHTRQVRMG
                     NLAFLGSRRVNGVSALHTQLMRKTVFRDFNAIYPDRIVNKTNGITFRRWLFQANPGLT
                     NLIVEAVGAQFLDSPGEIGKLAPFADDAAFRARFAANRRANKVSLARLIAERTNISVD
                     PEALFDAQIKRIHEYKRQLLNLLDIIALHHEMRANPAHDWVPRVKIFAGKAAAGYAQA
                     KLIIKLANDVERVVNGDPSLRGLLKVVFLANYNVSLAEAIVPAADLSEQISTAGMEAS
                     GTGNMKLALNGALTIGTLDGANIEIREQVGEENLFIFGLRAEQVDERRRGGLDATAAI
                     QASPALSGVLEALESGRYSPDDHTLYRNLVDNLRHHDYFMVCADFEAYRAAQRAVDDL
                     WRDPAAWSRRAILNTAGMGWFSADRAILEYAREIWRAEPGTP"
BASE COUNT          340 a          733 c          893 g          389 t
ORIGIN      
        1 ctccgctacg gcgtccccgg ctcggcgcgc cagatctcgc gcgcgtattc gaggatcgcg
       61 cggtcggccg agaaccagcc catgccggcg gtgttgagga tggcgcgccg cgaccaggcg
      121 gccgggtcgc gccagaggtc gtcgacggcg cgctgggcgg cgcggtacgc ctcgaagtcc
      181 gcgcagacca tgaagtagtc gtggtggcgc aggttgtcga cgagattgcg gtagagcgtg
      241 tggtcgtccg gggagtaccg gcccgactcg agcgcctcga gcaccccgga aagcgcgggc
      301 gaggcctgga tggccgccgt cgcgtcgagg ccgccgcggc gtcgctcgtc aacctgctcg
      361 gcgcgcaggc cgaagatgaa caggttctcc tcgcccacct gctcgcggat ctcgatgttg
      421 gcgccgtcga gcgtgccgat ggtgagcgcg ccgttcagcg cgagcttcat gttgcccgtg
      481 cccgaggcct ccatgcccgc cgtggagatc tgctcggaga ggtccgccgc ggggactatc
      541 gcctcggcga gactgacgtt gtagttggcc aggaagacga ccttgaggag gccgcgcagc
      601 gaagggtcgc cgttcaccac gcgctcgacg tcgttggcga gcttgatgat gagcttggcc
      661 tgggcgtagc ccgccgcggc ctttcccgcg aagatcttga cgcgcggcac ccagtcgtgc
      721 gccgggttgg cccgcatctc gtggtgcagc gcgatgatgt cgagcaggtt caggagctgg
      781 cgcttgtact cgtggatgcg cttgatctgg gcgtcgaaga gcgcctccgg gtcgacactg
      841 atgttggtgc gttcggcgat gaggcgcgcg agggacacct tgttggcgcg ccggttggcg
      901 gcgaagcggg cgcggaacgc ggcgtcgtcc gcgaaaggcg cgagcttccc gatctccccg
      961 gggctgtcga ggaactgcgc gccgacggcc tcgacgatga ggttggtgag gccggggttc
     1021 gcctggaaca gccagcggcg gaaggtgatg ccgttggtct tgttgacgat tcgatcgggg
     1081 tagatcgcgt tgaagtcccg gaagaccgtc ttgcgcatga gctgcgtatg cagggcggac
     1141 accccgttca cgcgccgcga cccgaggaac gcgaggtttc ccatgcgcac ctggcgggtg
     1201 tggttctcct cgatcagcga gagcgacgcg aggagccgcg tgtcgttgcg gccggctgcc
     1261 cgcaggctgt ccaggtgccg cgcgttgatg aggaagatga tctgcatgtg ccgcggaagg
     1321 aggccttcca tcagcggcac cgaccatttc tcgagcgcct cgggcagcag ggtgtggttg
     1381 gtgtagctga agaccgaggt gcagatccgc cacgcgtggt tccacggcat cccgtgctcg
     1441 tcgacgagca ggcgcatcag ctcggcgacg gcgatcgccg ggtgcgtgtc gttgagctgg
     1501 atcgccacgt ggtcgggcag cgaagagatc tcgccgtgct gctgcttgtg gcggcgcagc
     1561 aggtcctgca gcgacgcgga ggcgaagaag atttcctggc gcaggcgcag ctcgtgcccg
     1621 gcgggcgtgt cgtcgctcgg gtagagcacg cgcgagatcg cctcgagacg cacgcggtcg
     1681 gcgagagcgc ccacgtggtc gccgcggttg aaatcctcga gcacgatcgg gtggttggaa
     1741 cgggccgacc agaggcggag cgtgttgacg tgcgcgccgc gccagccggc gatgggcgtg
     1801 tcgtaggcca ccgcgttgac gctgtcggcg ggacgccaca cggaccgggt ggtgccgtcc
     1861 acgttgggga cggcatcgac cgcgccgccg aagccgatgg tgtagctgac ctcgggccgc
     1921 tcgaactccc acgggttgcc cgacgacagc cagttctcgg gcaactcgat ctgccagccg
     1981 ttcttgatcg cctggcggaa gatgccgtgg tcgtagcgga tgccgtagcc gtgcgcgggt
     2041 atgcccagcg tggacatgct ctccatgaag caggccgcga gccgccccag gccaccgttg
     2101 cccagcgcgg cgtcgggctc cagcttgcgc agccggtcga ggtcgatgcc caggtcggcc
     2161 agcgcctcgc gcgccgtcgc gacgagcccg aggttgccga gggaatcgag gagcagccgc
     2221 ccgatgagga attcgaggga gaagtagtag acgcgcttgc ggccgtcgcg gtaggtcttg
     2281 cgcgtggcct ccatccagcg ttccacgatc tggtcgcgcg tggcgagcgc cgtggcgatg
     2341 agccagtcgt ggtcg
//
LOCUS       DASUEV010000161         4173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_150996, whole genome shotgun sequence.
ACCESSION   DASUEV010000161 DASUEV010000000
VERSION     DASUEV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150996"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFV55_05250"
     CDS             <1..378
                     /locus_tag="VFV55_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HEX4943738.1"
                     /translation="EPPEFCGQFGLPWIRAVRVGKGVDLIECASHFANAAALLLDAQV
                     PGQAGGTGTAFDWSLVPPGLARPVVLSGGLDAGNVGRAIAAVRPWAVDVSSGVEVERG
                     VKDASRIEEFVRRVRDADARAGG"
     gene            359..1588
                     /gene="trpB"
                     /locus_tag="VFV55_05255"
     CDS             359..1588
                     /gene="trpB"
                     /locus_tag="VFV55_05255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX4943739.1"
                     /translation="MRAPAVEPRSPAAYDLPDATGHFGPYGGVFVAETLQAALGELRE
                     AYEACRKDPAFQSELAYELEHYVGRPSPVYHARRLSDSLGGAQIYLKREDLNHTGAHK
                     VNNTMGQALVARRMGKPRVIAETGAGQHGVAAATVAARYGMECVVYMGTEDVARQAQN
                     VYRMKLLGATVIPVTSGSRTLKDALNEAMRDWVTNVANTFYIIGTVAGPHPYPMMVRD
                     FNAVVGKECLVQMPALAGRQPDFVLACVGGGSNAMGIFHPYIGNEDVRLIGVEAGGLG
                     IATGKHAATLSAGSPGVLHGNRTYLMQDADGQILETHSISAGLDYPGVGPEHAWLKDS
                     GRAQYVSVTDDEALAAFHALCRTEGIIPALESSHAVAHAMKMAPALGKDRILLVNLSG
                     RGDKDMHTVAKASGITF"
     gene            1623..2444
                     /gene="trpA"
                     /locus_tag="VFV55_05260"
     CDS             1623..2444
                     /gene="trpA"
                     /locus_tag="VFV55_05260"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX4943740.1"
                     /translation="MSRIAATFARLRAGRRAALVPFVTAGDPTPAHTLELLRALVRNG
                     ADVVELGVPFSDPMADGPTIQRASDRALRAGTTLAMVLDAVKQYRREDDRTPIVLMGY
                     ANPVEHMGMASFAARAKDAGVDGVLIVDYPPEESGTWVAALAQAGIDPIFLLSPTSSD
                     ARIDLVASLARGYIYYVSLKGVTGAANIDTADVERMLGRIRTRTSVPVGVGFGIRDPQ
                     TARNVARFADAVIIGSAIVEEIERAEPDQAAARAGATLAEFRAGIDAAAGDRNAA"
     gene            2441..3310
                     /gene="accD"
                     /locus_tag="VFV55_05265"
     CDS             2441..3310
                     /gene="accD"
                     /locus_tag="VFV55_05265"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX4943741.1"
                     /translation="MSWFRKLLPPKIKREEGTHRKAVPEGLWSKCPSCEAVLYFTDLE
                     KNLHVCPKCGFHNRLTSRQRLDSFLDPEPRAELASEVVPLDPLKFKDSRKYTERLAEA
                     ERETGETDALVVMQGTVKSVPVIAAAFEFEFLGGSMGSVVGERFIRAVQACCDERVPY
                     VCFTATGGARMQEGLLSLMQMAKTTASLHQLSAARQPFISVLTDPTMGGVSASFAMIG
                     DVVIAEPGALVGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDMIVDRREMRDRVSR
                     LIALLKREPAPQA"
     gene            3339..>4173
                     /locus_tag="VFV55_05270"
     CDS             3339..>4173
                     /locus_tag="VFV55_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX4943742.1"
                     /translation="MSRPAPPGAAAPLGAWLDYISAQHPAQIALGLERVRDVMDRMGV
                     VPPAFAITVGGTNGKGSTCAFLECILRTAGYRTGLYTSPHLVRYNERVRIDGEEAQDA
                     NLARDFARVEAARGATPLTYFEFGTLAALAAFEKAEVDVAILEVGLGGRLDAVNIVDA
                     DCSIVSSVDFDHQAWLGDTREAIGLEKAHIFRAARPAFFGDTDPPASLVGHAAAIGAD
                     LQVLGRDFRAVPHERQWDFEGRRGARRALPMPALRGPWQLANAATALAALDEVSDRFP
                     VS"
BASE COUNT          632 a         1458 c         1434 g          649 t
ORIGIN      
        1 gagccgccgg aattctgcgg ccagttcggg ctgccctgga tccgcgccgt ccgggtaggc
       61 aagggagtcg atttgataga atgtgccagt catttcgcga acgcggcggc cttgctgctc
      121 gatgcgcagg tgcccggaca ggcggggggt accggtactg cgttcgactg gtccctcgtc
      181 ccgccagggc ttgcacgacc cgtggtgctg tcgggcgggc tggatgccgg caacgttggc
      241 cgtgccatcg ccgcggtaag gccctgggcg gtggacgttt ccagcggcgt cgaagtggag
      301 cgcggcgtga aggacgcgtc ccggatagaa gaattcgtga ggagagtgag agatgcagat
      361 gcgcgcgccg gcggttgagc cccgctcccc cgcggcctat gacctgcccg acgcgaccgg
      421 ccatttcgga ccgtacggcg gcgtcttcgt cgccgaaacg ctgcaggcgg cgctgggaga
      481 gcttcgcgag gcctacgagg cttgccgcaa ggaccctgca ttccagtcgg agctcgccta
      541 cgagctcgag cattacgtcg ggcgccccag ccccgtgtat cacgcgcggc ggctttcgga
      601 ctcgctgggc ggcgcccaga tctacctgaa gcgcgaggac ctcaaccata ccggcgccca
      661 caaggtgaac aacaccatgg gccaggccct cgtcgcccgg cgcatgggca agccgcgggt
      721 gatcgcggag acgggcgctg gccagcacgg cgtggccgcc gcgaccgtgg ctgcacgcta
      781 cggcatggaa tgcgtcgtgt acatgggcac ggaggacgtc gcgcgccagg cgcagaacgt
      841 ctaccgcatg aagctcctcg gcgcaaccgt gatccccgtc acgagcggat cgcgaactct
      901 caaggacgcg ctcaatgaag cgatgcgcga ctgggtgacc aacgtcgcga acacgttcta
      961 catcatcggg accgtcgccg gcccgcatcc ctatccgatg atggtgcgcg acttcaatgc
     1021 cgtcgtcggc aaggagtgcc tcgtgcagat gcccgcgctt gccgggcggc agcccgactt
     1081 cgtgctggcc tgcgtcggcg gcggctcgaa cgcgatgggc atcttccatc cctacatagg
     1141 gaacgaggac gtgcggctca tcggcgtgga ggccggcggc ctcggcatcg ccaccggcaa
     1201 gcacgcggcc acgctttcgg ccggttcgcc cggcgtcctg cacggcaacc gcacgtacct
     1261 gatgcaggac gcggacggcc agatcctcga gacgcattcg atctcggcgg gcctcgacta
     1321 tcccggcgtc ggcccggagc acgcctggct gaaggattcc ggccgcgccc agtatgtctc
     1381 ggtgactgac gacgaggcgc tcgccgcgtt ccacgcgctg tgccggaccg aaggcatcat
     1441 ccccgcgctc gaatcgagcc atgcggtggc ccacgcgatg aagatggcgc cggcgctggg
     1501 caaggatcgc atcctgctgg tgaatctgtc cgggcgtggc gacaaggaca tgcacaccgt
     1561 cgccaaggct tcgggcatta cgttctagtg tcggctcgtt cgtcgaccat gccgggaatc
     1621 ccatgagccg catcgcagcc actttcgcca gacttcgcgc cggacgccgc gcggccctcg
     1681 tgcccttcgt cacggccggc gacccgacgc ccgcccatac cctcgagctg ttgcgcgcgc
     1741 tggtccggaa cggcgcggac gtagtcgagc tgggcgtgcc cttttccgac cccatggccg
     1801 acgggccgac catccagcgc gcctcggatc gcgcccttcg cgcgggcacg acgctcgcga
     1861 tggtcctcga tgcggtgaag cagtaccgcc gcgaggacga ccgcacgccg atcgtgctga
     1921 tgggctatgc gaacccggtc gagcacatgg ggatggcgag cttcgcggcg cgcgcgaagg
     1981 atgccggcgt ggacggtgtc ctcatcgtcg actatccccc ggaggaaagc ggaacgtggg
     2041 tcgcggcgct cgcccaggcg ggcatcgacc cgatcttcct gctttcgcca acgtcgtcgg
     2101 acgcgcgcat cgacctggtc gcgagcctgg ccaggggcta catctattac gtttcgctga
     2161 agggcgtcac cggtgccgcc aacatcgaca cggcggacgt ggaacgcatg ctcgggcgca
     2221 tccgcacgcg cacctcggtg cccgtgggcg tgggattcgg cattcgcgat ccgcagaccg
     2281 cgcgcaacgt cgcgcgcttc gccgacgcgg tgatcatcgg cagcgccatc gtcgaggaga
     2341 tcgagcgcgc cgagccggac caggccgccg cgcgggcggg cgcgacgctc gccgagtttc
     2401 gcgccggcat cgatgcggcg gctggcgaca ggaacgccgc gtgagctggt tccgcaagct
     2461 cctgcccccc aagatcaagc gcgaggaggg cacccaccgg aaggcggttc ccgagggcct
     2521 gtggagcaag tgcccatcgt gcgaggcggt gctctatttc accgacctcg agaagaacct
     2581 gcacgtctgc ccgaagtgcg gcttccacaa ccggctcacg tcccgccagc gcctcgattc
     2641 attcctcgat ccggagccgc gcgccgaact cgcttccgag gtcgtaccgc tcgacccgct
     2701 caagttcaag gacagccgga aatacaccga gcggcttgcc gaggccgaga gggaaaccgg
     2761 ggagaccgat gcgctcgtcg tcatgcaggg gaccgtgaaa tcggtgcccg tcatcgcggc
     2821 ggccttcgag ttcgagttcc tgggcggctc catgggctcg gtggtcgggg agcgtttcat
     2881 ccgcgccgtg caggcgtgct gcgacgagcg cgtgccctat gtctgcttca cggcgaccgg
     2941 cggcgcgcgc atgcaggaag gactgctgtc gctcatgcag atggcgaaga cgacggcctc
     3001 gctgcaccag ctttccgccg ccaggcagcc gttcatctcg gtgctcaccg atcccaccat
     3061 gggcggcgtg tcggcaagct tcgccatgat cggcgacgtg gtgatcgccg agcccggcgc
     3121 cctcgtgggc ttcgcggggc cgcgggtcat cgagcagacg gtgcgcgaga cgctgccgga
     3181 aggattccag cgcgccgaat tccttctcga gaagggcgcg atcgacatga tcgtcgaccg
     3241 gcgcgagatg cgcgaccggg tctcgcggct catcgccctg ctcaagcgcg agccggcgcc
     3301 gcaggcgtga gcgcgtcccg ggccgcgcgc cccgcgcggt gagccgcccg gccccgcccg
     3361 gcgccgccgc tccgctgggg gcgtggctcg actacatctc cgcccagcat cccgcgcaga
     3421 tcgcgcttgg cctcgaacga gtgcgcgacg tgatggatcg catgggcgtc gtgccgccgg
     3481 ccttcgcgat caccgtgggc gggaccaacg gaaagggctc cacctgcgcg ttcctggaat
     3541 gcatcctgcg caccgcgggc taccgtaccg gcctctacac ctccccgcac ctcgttcgct
     3601 acaacgagcg cgtgcgcatc gacggcgagg aggctcagga cgcgaacctt gcgcgcgact
     3661 ttgcgcgcgt ggaggcggcg cgcggcgcca cgccgctgac ctatttcgaa ttcggcacgc
     3721 tcgccgcgct ggctgccttc gaaaaggcgg aggtggacgt cgccatcctc gaagtgggcc
     3781 ttggcggccg tctcgacgcg gtcaacatcg tcgatgccga ctgctcgatc gtctccagcg
     3841 tggatttcga ccaccaggcg tggctgggcg atacccgcga ggcgatcggc ctggaaaaag
     3901 cccacatctt ccgtgccgcg cgcccggcct tcttcggcga caccgatcct ccggcttccc
     3961 tcgtcggcca cgccgccgcg ataggcgcgg acctgcaagt gctcggccgc gacttccgcg
     4021 ccgttcccca cgagcggcaa tgggatttcg aaggccggcg cggcgccagg cgcgcgctgc
     4081 ccatgccggc cctgcgcggg ccgtggcagc ttgcgaacgc tgccaccgcg cttgcggccc
     4141 tcgacgaggt gtcggaccgt ttccccgtct cgc
//
LOCUS       DASUEV010000162         4596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_151303, whole genome shotgun sequence.
ACCESSION   DASUEV010000162 DASUEV010000000
VERSION     DASUEV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151303"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFV55_05275"
     CDS             <1..225
                     /locus_tag="VFV55_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4943743.1"
                     /translation="FGIRVVTIAPGIFDTPMLQGMSEELRASLGAQVPFPPRLGRPDE
                     YAALVAHIIENEVLNGEVIRLDGAIRMGAK"
     gene            389..1555
                     /locus_tag="VFV55_05280"
     CDS             389..1555
                     /locus_tag="VFV55_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX4943744.1"
                     /translation="MPFDIDLPEELDALRASVRRLCDAELSPRAAEIDRANEFPADMW
                     ARLGGLGLLGMTVPEEYGGTGLGYLAHVVAMEEVSRASASVGLSYGAHSNLCVNNLFL
                     NGSEAQRRKYLPGLCSGEFVGALAMSEAGAGSDVVGSMACRAERKGDRWVANGSKMWI
                     TNGPDANVLIVYMRTAPKEQGSRAMTAFLVEKGMKGFSTAQKLDKLGMRGSNTCELVF
                     ADCEIPEENILGQVNGGSHVLMKGLDTERCVLSGGPIGIMQAALDLVIPYAHERKQFG
                     QPIGTFELMQGKLADMYVKLQSSRAFSYRVAEALDKGRGRAARKDAAACLLFSSENAV
                     QVALEAIQALGGNGYINDYPAGRLLRDAKLYDIGAGTNEIRRMLIGRELFEESA"
     gene            1596..2780
                     /locus_tag="VFV55_05285"
     CDS             1596..2780
                     /locus_tag="VFV55_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HEX4943745.1"
                     /translation="MAARDVVVVSGARTAIGDYGASLKDVPPTKLGSIVIREALARAK
                     IDPATVGHVVLGSVIHGEVRDMYISRVAAVDAGVPVGTPCLTVNRLCGSGLQAIVSAS
                     QHILLGDIDVAIGAGAESMSRAAYFLPAARWGQRMGDGQIIDAMVGALTDPFGTGHMG
                     ITAENLAAKHGFTREQQDAFSLESHRRAAAAIDKGNFTSQIVPVEVKTKKGPVQFAVD
                     EHVRRDAKAEDFSKLRPAFKKDGTVTAGNASGINDAAAAVVLMEAGAASKAGVKPLGR
                     LVAYAHAGVEPHIMGEGPIPAVQKVFQKAGLKPGDMDVIESNEAFAVQAMTVNKQLGL
                     DPAKVNPNGGAVALGHPIGATGCILTVKCLYELQRTNGRYGLVTMCIGGGQGIAAIFE
                     RL"
     gene            2837..4015
                     /locus_tag="VFV55_05290"
     CDS             2837..4015
                     /locus_tag="VFV55_05290"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX4943746.1"
                     /translation="MSESIVIVGAKRTPIGAMQGKFNSLAAHQLGSVAIKAALEQAGV
                     GPKEVDDLIFGCVLSAGQGQAPARQAALGAGLDKSTPCTTVNKMCGSAMKATMMGADE
                     ILAGGATVVVAGGMESMTNAPHLLPKARAGYRYGHQKVLDHMAFDGLENAYDGKPMGF
                     FADKTAEKYGFTREQMDDYAKESTARAVNASKSGLFTDEIAPVTVAGRKGDEVVKDDE
                     TPFTVNVEKIPQLRPAFNKDGVVTAATSSSISDGAAALVLMTETEAKKRGAKPLARIV
                     AHASNAHEPEWFTTAPVGAIKKVLAKAGWKAADVDLYEVNEAFAVVTMAAMKDIGIGA
                     EKVNVNGGAVALGHPIGATGARIITTLIYALKKRGLKKGVAALCIGGGEATAVALEVL
                     "
     gene            4024..4290
                     /locus_tag="VFV55_05295"
     CDS             4024..4290
                     /locus_tag="VFV55_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEX4943747.1"
                     /translation="MNLTPIESTPGIGPNLACELRRVGIDSLEALLRVGFWDAWQQVR
                     RANPERDCLPSCLALAGAVAGVRWNHLPPKVRADIRQRVKAARA"
     gene            4290..4523
                     /locus_tag="VFV55_05300"
     CDS             4290..4523
                     /locus_tag="VFV55_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943748.1"
                     /translation="MGRHGVAVLVLLAAAIAGCGKEAPKADAATERKEALERAKEGAF
                     GAPVKALEDAKGMGADLDRKARDAVDKAEQDAK"
BASE COUNT          822 a         1580 c         1534 g          660 t
ORIGIN      
        1 ttcggcatcc gcgtggtcac catagcgccc ggcatcttcg atacgccgat gctccagggc
       61 atgagcgagg agcttcgcgc ctccctcggc gcgcaggtcc ccttcccgcc ccgcctgggg
      121 cgcccggacg aatacgcggc actcgtcgcg cacatcatcg aaaacgaagt gcttaacggg
      181 gaggtaatcc gcctcgacgg cgccattcgc atgggcgcca agtaggtcca gctccgcttc
      241 agcaacgggc agcgcgtccg gccctcgcga ataacgcccg cagggggcaa cagcgaggca
      301 ccgtttcgag cgataattac ggtttgggat cgcccccccg gggcggaccc cccgcccctc
      361 cccggggctg caccagattg gagcccactt gccgttcgac atcgatctcc ccgaggagtt
      421 agacgcgctt cgcgccagcg tgcgtcgcct gtgcgatgcc gaactctccc cccgcgccgc
      481 ggagatcgac cgggcgaacg aatttcccgc cgacatgtgg gccaggctgg gcggcctggg
      541 cctgctcggc atgacagtgc cggaggagta tggcggcacc ggcctgggct atctcgccca
      601 cgtcgtggcc atggaagagg tttcccgtgc ctcggcctcg gtgggcctgt cctatggggc
      661 gcactcgaac ctgtgcgtca acaatctctt cctcaacggc agcgaggcgc agcggcgcaa
      721 gtacctgccc gggctttgct cgggcgaatt cgtgggcgcc ctcgcgatga gcgaggcggg
      781 tgccgggtcc gacgtcgtgg gctccatggc gtgccgcgcg gaaaggaagg gcgatcgctg
      841 ggtcgccaac ggctcgaaga tgtggatcac caacgggccc gacgcgaacg tcctcatcgt
      901 ctacatgcgc accgccccga aggaacaggg ctccagggcg atgaccgcct tcctggtcga
      961 gaaaggcatg aagggattca gcaccgccca gaagctggac aagctgggca tgcgcggctc
     1021 caatacctgc gagctcgttt tcgccgattg cgagatcccc gaggaaaaca tcctgggcca
     1081 ggtgaacggc ggctcgcacg tgctcatgaa gggcctggac accgagcgtt gcgtcctgtc
     1141 cggggggccc atcggcatca tgcaggcggc gctggacctc gtgattccct acgcccacga
     1201 gcgcaagcaa ttcggccagc ccatcggaac cttcgagctg atgcagggca agctcgccga
     1261 catgtacgtg aagctgcaat cgtcgcgcgc cttcagctac cgcgtcgccg aagccctcga
     1321 caagggccgc gggcgcgcgg cgcgcaagga tgccgccgcg tgcctcctct tttcctcgga
     1381 gaacgccgtt caggtggcgc tggaagccat ccaggccctc ggcggcaacg gctacatcaa
     1441 cgactacccg gcagggcggc tccttcgcga cgcgaagctc tacgacatcg gcgcgggaac
     1501 caacgagatc cgcaggatgc tcatcggtcg cgagctgttc gaggaaagcg cctgaaccgg
     1561 cccggaaact tcccccctga aacaggagaa cgacgatggc tgcacgtgat gtggttgtag
     1621 tttccggcgc acgtaccgcg atcggcgatt acggtgcctc cctgaaggac gtcccgccga
     1681 cgaagctcgg ttcgatcgtg atccgggaag ccctggcgag ggcgaagatc gatccggcca
     1741 cggtcgggca cgtggtgctg ggcagcgtca tccacggcga ggtgcgcgac atgtacatct
     1801 cgcgcgtggc cgccgtcgac gccggcgttc ccgtcggcac gccgtgcctc accgtgaacc
     1861 gcctgtgcgg ctccggactg caggcgatcg tctcggcctc gcagcacatc cttctgggcg
     1921 acatcgacgt ggccatcggc gccggcgccg agagcatgag ccgcgcggcc tatttcctcc
     1981 ccgccgcccg ctggggacag cgcatgggcg acggccagat catcgacgcg atggtggggg
     2041 cgctgaccga cccgttcggc accggccaca tgggcatcac cgcggaaaac ctcgccgcca
     2101 agcacggctt cacgcgcgag cagcaggacg cgttctcgct cgagtcgcac cgccgcgcgg
     2161 ctgccgcaat cgacaagggt aacttcacca gccagatcgt gccggtcgag gtgaagacga
     2221 agaaggggcc ggtgcagttc gccgtcgacg agcacgtccg ccgcgacgcg aaggccgagg
     2281 atttctccaa gctgcgcccg gcgttcaaga aggacggcac ggtgacggcc ggcaacgcct
     2341 ccggcatcaa cgacgccgcc gccgcggtcg tcctcatgga agccggcgcc gccagcaagg
     2401 cgggcgtgaa gccgctgggg cgcctcgttg cgtacgcgca cgccggcgtg gagccgcaca
     2461 tcatgggcga aggcccgatc cccgcggtgc agaaggtgtt ccagaaggcc ggcctcaagc
     2521 ccggcgacat ggacgtgatc gaatccaacg aggccttcgc ggtgcaggcc atgaccgtga
     2581 acaagcagct gggcctcgac cccgcgaagg tgaacccgaa cggcggcgcg gtggccctcg
     2641 gccacccgat cggcgctacg ggctgcatcc tcacggtcaa gtgcctttac gagctgcaac
     2701 gcacgaacgg ccgctacggc ctcgtcacga tgtgcatcgg cggcggccag ggcatcgccg
     2761 ccattttcga acgcctttga tccgcaccac tcgcctcaaa gaccgcctca aggactgaga
     2821 tagaaaggaa gcacgcatgt cggaatccat cgttatcgtc ggcgccaagc gaacccccat
     2881 cggcgccatg caaggcaagt tcaactccct cgctgcccac caactcggct ccgtggccat
     2941 caaggctgcc ctcgagcagg ccggcgtcgg cccgaaggag gtggacgatc tcatctttgg
     3001 ctgcgtcctg tccgccgggc agggccaggc gcccgcgcgc caggccgcgc tcggcgccgg
     3061 cctcgacaag tccacgccgt gcaccaccgt caacaagatg tgcggctcgg ccatgaaggc
     3121 cacgatgatg ggcgcggacg agatcctcgc cggtggtgcc acggtcgtgg tcgccggcgg
     3181 catggaatcg atgaccaacg cgccgcacct gctcccgaaa gcgcgcgcgg gctaccgcta
     3241 tggccaccag aaggtgctcg accacatggc cttcgacggg ctcgagaacg cctacgacgg
     3301 caagcccatg ggtttcttcg ccgacaagac ggccgagaaa tacggcttca cgcgcgagca
     3361 gatggacgac tacgcgaagg aatcgaccgc gcgcgcggtc aacgcctcga agtccggcct
     3421 cttcaccgac gaaatcgcgc ccgtcaccgt ggccggccgc aagggcgacg aagtggtgaa
     3481 ggacgacgag acgcccttca ccgtgaacgt cgagaagatc ccgcagctgc gtcccgcctt
     3541 caacaaggac ggcgtcgtga cggccgccac ctcgtcatcg atttccgatg gcgccgcggc
     3601 gctggtgctg atgaccgaga ccgaggcgaa gaagcgcggc gccaagcccc tggcgcgcat
     3661 cgtcgcgcac gcctccaacg cccacgagcc ggaatggttc accacggccc cggtcggcgc
     3721 gatcaagaag gtgcttgcca aggccggctg gaaggccgcc gacgtggacc tctacgaggt
     3781 gaacgaggcc ttcgccgtag tcaccatggc cgccatgaag gacatcggca tcggcgcgga
     3841 aaaggtcaac gtgaacggcg gcgcggtcgc gctcggccac ccgatcggcg ccacgggcgc
     3901 gcgcatcatc acgaccctca tctacgcgct gaagaagcgc ggcctcaaga agggcgttgc
     3961 cgccctgtgc attgggggag gcgaggccac ggccgtcgca ctggaggtcc tatgaactcg
     4021 acgatgaacc tgactcccat cgaaagcacc cccggtatcg gccccaatct cgcctgcgag
     4081 cttcgccgag tcggcatcga ctccctcgaa gcgctcctgc gggtgggctt ctgggacgcg
     4141 tggcagcagg tccgccgcgc caacccggaa cgcgactgcc tgccgtcgtg ccttgccctc
     4201 gcgggtgcgg tagcgggcgt tcgctggaat catcttccgc cgaaggtgcg cgccgacatc
     4261 cggcaacggg tcaaggccgc gcgcgcgtga tgggtcgcca cggcgtggcg gtcctggtgc
     4321 tgctcgccgc cgcgatcgcg ggctgcggga aggaagcccc gaaggccgat gccgccaccg
     4381 agcgcaagga ggcgctcgag cgcgccaagg aaggcgcgtt cggcgcgccg gtcaaggcgc
     4441 tggaggatgc caaggggatg ggagccgatc tcgacaggaa ggcgagggac gccgtcgaca
     4501 aggccgaaca ggacgcgaag tgagcctgcc tgcatgctga accgcgtcca ggtcgatttc
     4561 ctgaccgaag agcagcggat gattcgcgac accgcg
//
LOCUS       DASUEV010000163         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_47173, whole genome shotgun sequence.
ACCESSION   DASUEV010000163 DASUEV010000000
VERSION     DASUEV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47173"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1759)
                     /locus_tag="VFV55_05305"
     CDS             complement(410..1759)
                     /locus_tag="VFV55_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX4943749.1"
                     /translation="MREAIDSAVRDRADQFVATLLESVRIPSVSLTGQGIGEAVDFLS
                     ARMKRWGFEVQVFPTDSQPIVYGEIGPRNAPFTWLTYGHYDVYPADAAAEGWKTDPWQ
                     PVVDGDRIRGRGTGDNKGQHLALLNAIALYRELEGELPIRIKVILEGDEETGSRPLPA
                     FVEKHRDMLRADLCCYSDGPMFPNDQPVLLMGVRGILGLEFVASGARRNLHSGNFGGV
                     APNPTLDLIHLLAAMVDRDGRLLVPGAGYADVTVTPAEAAAARALPLDREGVMESMGV
                     PPTVPDGEFHERLMLRPAFNVSGFASGYTGPGHKTIIFREALAKADIRLVGGQDPAQV
                     LAAVRKFAADRGHAGIEIRELKSTPASRTSLDSPFIPVVKSAVAKGFGREPLVVPSLG
                     GTTPDYVFTRLLGLPSIVVPLAPYDENNHAPNESTKVSLYLAGIRSGMRLIEAFGNR"
     gene            complement(1756..2145)
                     /locus_tag="VFV55_05310"
     CDS             complement(1756..2145)
                     /locus_tag="VFV55_05310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4943750.1"
                     /translation="MRTLESLNAPGLPPPAGHYSHAMVHDGIAYLAGQLPHVPGDPGR
                     QPGTIEEQARQVLENVDRVLQACGSRRNRVIRCTVYVSDIAHWPAVNKVYAEFFGEHR
                     PARAVVPVNELHHGYAIEIEVTAAVDA"
     gene            complement(2182..3297)
                     /locus_tag="VFV55_05315"
     CDS             complement(2182..3297)
                     /locus_tag="VFV55_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX4943751.1"
                     /translation="MRLSDLETPCLVLDAGRMEANVARLKAHLADLGGHLRPHVKTCK
                     SIEVARGLMETPTGPITVSTLKEAEQFLDHGVTDILYAVGIAPGKLDHVLALRKRGAD
                     LAVVVDSVEAAQAVAARNARIPVLIEVDCDGHRAGVKPGDPVLVEIAKALARGAQPRG
                     VMTHAGDSYNCRSTEAIAAMAEKERVAVLGSAMVLRSAGFASPVVSVGSTPTATFARS
                     LAGVTEVRAGVFVFHDLVMAGLGVCRIEDIALSVLATVIGHQREKGWVITDGGWMAMS
                     RDRGTARQPMDQGYGLVCDEAGRPLGDLVVSDANQEHGIVSRRGGGPVDVSRYPVGTR
                     LRILPNHACATGAQHDAYHVVRGGTEVVARWPRFSGW"
     gene            complement(3320..>3678)
                     /locus_tag="VFV55_05320"
     CDS             complement(3320..>3678)
                     /locus_tag="VFV55_05320"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEX4943752.1"
                     /translation="IALPPKIYLVNWFRKDDEGKYVWPGYGENMRVLKWIVERVEGLA
                     DAERTAVGYVPRHKDIDWRGMDSFGADKYLAITAIDKAMWKQEMPLHREFFDKLGERL
                     PAVMRQRYESLAGLLG"
BASE COUNT          552 a         1258 c         1258 g          610 t
ORIGIN      
        1 cctcggattt ttacccatct aatgacgagg gcgctgcgct gcctcgtccg cgacggtcgc
       61 gtgccaagga ttgcgatcga cttccaccgc caccgcctcc cacgctccgt ggatacagca
      121 ccgagtgaga caacgcctga ccgcgctcgt ccgccgctga aagcgccacg cggcctcttc
      181 atgggggcca gggatggggc aagtctatcg gcgtccttgg gcacgcgacc gtcgggatgg
      241 aagagcgcag cggctccatc tctcaatggg taaaaagtcg gggacagacg tgtttggctg
      301 gccccgactt tttgggaccc atccgacggt ctcgcttgcg tgccccagac gctgagagac
      361 ttgccccgtc ccatagagtg tgggcggccg tttccgcgct gccccgcagt taccggttgc
      421 cgaaagcctc gatgagccgc attcccgaac ggatccccgc caggtacagc gacaccttgg
      481 tcgactcgtt gggtgcgtgg ttgttctcgt cgtacggggc caggggcacc acgatggagg
      541 gaaggcccag cagcctggtg aagacgtaat cgggcgtggt cccgcccagg ctcggcacca
      601 ccaggggttc gcggccgaat cccttcgcga cggcagactt cacgaccggg atgaacgggc
      661 tgtcgagcga cgttcgcgag gcgggggtgc tcttgagctc gcggatctcg attcccgcgt
      721 ggccccggtc ggccgcgaac ttgcgcacgg cggcgaggac ctgcgcaggg tcctgcccgc
      781 ccaccaggcg gatgtcggcc ttggcgagcg cttccctgaa gatgatcgtc ttgtgcccgg
      841 ggccggtgta gccggaggcg aagccggaga cattgaatgc cgggcgcaac atcaggcgtt
      901 cgtggaattc gccgtcgggc accgtgggcg gcacgcccat gctctccatc acgccctcgc
      961 ggtcaagcgg cagggccctc gccgcggccg cttcggccgg cgtcacggtc acgtcggcgt
     1021 atcccgcgcc cggcacgagc aggcgcccgt cgcgatccac catcgcggcc agcaggtgga
     1081 tgagatcgag cgtcgggttg ggcgccacgc cgccgaagtt tccggagtgg aggttgcgcc
     1141 gtgcgccgct ggccacgaat tccagcccca ggatgccgcg cacgcccatc agcaataccg
     1201 gctggtcgtt ggggaacatc ggcccgtcgg agtagcagca caggtccgcg cgcagcatgt
     1261 cgcgatgctt ctcgacgaag gcgggcagcg gccggctgcc cgtctcctcg tcgccttcga
     1321 gtatcacctt gatgcggatg ggcagctcgc cttcgagctc acggtacagc gcgatggcgt
     1381 tgagcagcgc caggtgctgc cccttgttgt cgcccgtgcc gcggccccgg atgcggtcgc
     1441 catcgacgac gggctgccag gggtcggtct tccacccttc ggccgccgcg tccgccggat
     1501 agacgtcgta gtgcccgtag gtgagccacg tgaacggcgc gttcctcggc ccgatctcgc
     1561 catagacgat gggttggctg tccgtgggga agacctgcac ttcgaagccc cagcgcttca
     1621 tgcgcgcgga aaggaaatcg acggcctcgc cgatgccctg gccagtgagg ctcaccgagg
     1681 gaatgcgaac cgactcgagc agcgtggcga cgaactggtc ggcgcggtcg cggacggcgc
     1741 tgtcgatcgc ctccctcatg cgtccaccgc ggcggtgacc tcgatctcga tcgcataccc
     1801 gtggtgcagc tcgttcaccg gcaccacggc gcgcgcgggg cggtgctcgc cgaagaactc
     1861 ggcatacacc ttgttcaccg ccggccagtg cgcgatgtcg gagacataaa cggtgcagcg
     1921 aatcacgcgg ttgcggcggc tgccgcaggc ctgcagcacc cggtccacgt tctcgagcac
     1981 ctggcgtgcc tgctcctcga tggttccggg ctggcgcccc gggtcgccgg gaacgtgcgg
     2041 caactgcccg gcgaggtagg cgatgccgtc atgcaccatg gcatgcgaat agtggccggc
     2101 gggcggcggc aaccccgggg cattgaggga ttcgagcgtc ctcatgtcga ctttctcctt
     2161 ggtgtctgac agcgcaatga tctaccagcc gctgaatcgc ggccagcggg cgacgacttc
     2221 ggtgccgccg cgcacgacgt gataggcatc gtgctgcgcg ccggttgcgc aggcgtggtt
     2281 gggcaggatg cgcaatcgcg tgccgacggg ataccgcgac acgtcgacgg gcccgccgcc
     2341 acggcggctc acgatgccgt gctcctggtt ggcatcggac acgaccaggt cgccgagcgg
     2401 tcggccggct tcgtcgcaca ccaggccgta gccctggtcc atgggctgcc tcgcggtgcc
     2461 gcggtcgcgc gacatcgcca tccagccgcc gtcggtgatg acccagccct tctcgcgctg
     2521 gtgaccgatg acggtggcca gcaccgaaag cgcgatgtcc tcgatgcggc acacgccgag
     2581 gcccgccatc accaggtcgt ggaagacgaa gacgccggcg cgcacctccg tcacgcccgc
     2641 gaggctcctg gcaaaggttg cggtgggtgt ggaacccacg ctcaccacgg gcgaggcgaa
     2701 gccggccgag cgcagcacca tggcgcttcc caggacggcg acgcgctcct tctccgccat
     2761 cgcggcgatc gcctccgtgg agcggcagtt gtaggaatcg cccgcgtgcg tcatcacgcc
     2821 acgcggctgg gcgcccctgg ccagcgcctt ggcgatttcg acgaggaccg ggtcgccggg
     2881 cttcacgccg gcccggtggc cgtcgcaatc gacctcgatg agcaccggaa tgcgcgcatt
     2941 gcgcgccgcc accgcctgcg cggcctcgac gctgtcaacg acgaccgcga gatccgctcc
     3001 gcgcttgcgc aaggcgagaa cgtggtcgag cttgcccggc gcgatgccca cggcgtagag
     3061 aatgtccgtg acgccgtggt ccaggaactg ctcggcttcc ttgagcgtcg ataccgtgat
     3121 cgggccggtg ggtgtctcca tcaggccgcg cgcgacctcg atggacttgc aggtcttcac
     3181 gtgcggccga aggtgcccgc ccaggtcggc gaggtgtgcc ttgaggcgtg cgacgttggc
     3241 ctccatgcgg ccggcgtcga ggacgaggca cggcgtttcg aggtcgctga gcctcatttc
     3301 cctctcgtca ccaggcaggt caccccagca atccggcgag cgactcgtat cgctgccgca
     3361 tcaccgccgg caggcgctcg ccgagcttgt cgaagaactc gcggtgcagc ggcatttcct
     3421 gcttccacat cgccttgtcg atggcggtga tggcgaggta cttgtcggcg ccgaaggaat
     3481 ccatcccccg ccagtcgatg tccttgtgcc ggggcacgta gcccacggcg gtgcgctcgg
     3541 cgtcggccag gccctcgacg cgctcgacga tccacttgag cacgcgcatg ttctcgccgt
     3601 agccgggcca cacgtacttg ccctcgtcgt ccttgcggaa ccagttgacg agatagatct
     3661 tgggcggcag ggcgatct
//
LOCUS       DASUEV010000164         4357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_151682, whole genome shotgun sequence.
ACCESSION   DASUEV010000164 DASUEV010000000
VERSION     DASUEV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151682"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="bamC"
                     /locus_tag="VFV55_05325"
     CDS             complement(<1..712)
                     /gene="bamC"
                     /locus_tag="VFV55_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX4943753.1"
                     /translation="MKRIRTLVLVLVPLVLASGCGIFKTEEQQRTARSRERPLEMPPD
                     LTTPTADERFAVPDPRASTSFSQYNRDRSAVAPGTPVSSSASGVLPAVPNAHIERGGD
                     QRWISAKADPAQVWPIVRDFWQDIGFSIARETPEAGIIETNWRDTRANVETTGIRGII
                     GRALPGMYSTGERDRFRTRLERGVEPGTTEIYVSHRGLEEVFTSINQDATRWQPRGSG
                     TDRDLEAEMLGRLLVKLGE"
     gene            complement(730..1608)
                     /gene="dapA"
                     /locus_tag="VFV55_05330"
     CDS             complement(730..1608)
                     /gene="dapA"
                     /locus_tag="VFV55_05330"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX4943754.1"
                     /translation="MLTGSLVAIVTPMQADGALDIPRFKSLLDWHIAEGTDGVVVVGT
                     TGESPTVDVDEHCLLIRTAVEHCAGRIPVVAGTGGNSTREAIELTKFARDVGANYSLS
                     VVPYYNKPAQEGLYRHFKAIAESVDLPMILYNVPGRTVADMSTDTTLRLAQVPGIVGI
                     KDATSNLDRGADLLRRKPAQFMVYSGDDATALPYMLLGAHGDISVTANVAPRAMHEMC
                     AAALAGEAKKAIALNQRLMGLHQKLFVEANPIPVKWALARMGRIGEGIRLPLTPLAPA
                     FHETVTGALREAGCLS"
     gene            complement(1664..2203)
                     /locus_tag="VFV55_05335"
     CDS             complement(1664..2203)
                     /locus_tag="VFV55_05335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4943755.1"
                     /translation="MTAHLADLPPSPWVARWASLVRPGGEVLDLACGSGRHARYLAAQ
                     GFEVTAVDRDPGLFPDPPGGVTLVGADVEAGPWPFAGRLFDAIVVTNYLHRPLLPVLV
                     QSLASGGVLVYETFALGNERFGKPSNPDFLLAPGELLEAVRGSLRVVGYEDLVVTVPR
                     PAAIQRICARREDPAVRGT"
     gene            2302..2742
                     /locus_tag="VFV55_05340"
     CDS             2302..2742
                     /locus_tag="VFV55_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX4943756.1"
                     /translation="MFLTIALCLALVVATTLVHFEMLTQLSARLPGIVIASRLKLVVV
                     MLTTFATHAIEIALYAVAIWSCIWLAGVGGLPGASGSTFVNCFYFSAETYTSLGFGDI
                     VPSGSLRLLAGVETLNGLLLIGWSSSYIYLSMERFWDVAPTAAP"
     gene            complement(2756..3871)
                     /gene="ald"
                     /locus_tag="VFV55_05345"
     CDS             complement(2756..3871)
                     /gene="ald"
                     /locus_tag="VFV55_05345"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX4943757.1"
                     /translation="MRVGVPKEIKVQEYRVGLTPSSVREVVERGHEVLVERGAGAGIG
                     AAEGAYEKAGARIVGSAEEVFAAADMIVKVKEPQAAEIALLRPGQILFTYLHLAPDPE
                     QCQGLLASGAVCIAYETVTQAGGGLPLLAPMSEVAGRMAVHAGAHCLEKAQGGMGILP
                     GGVAGVPPAKIVILGAGVVGSNAAQMAVGTGAVVVVIDRNTDALRRIDRQLGARVITV
                     FSNRDSVEHHVCSADMVIGGVLIPGAAAPKLVSRALVGEMKPGAVVIDVAIDQGGCFE
                     TSRATTHDEPTFLVGKVVHYCVANMPGAVPRTSTYALNNATLPFTLQLAGLGWKRALA
                     ADAHLMAGLNVCEGKVTHREVAEALGHAFHDPLSMLS"
     gene            4009..>4357
                     /locus_tag="VFV55_05350"
     CDS             4009..>4357
                     /locus_tag="VFV55_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEX4943758.1"
                     /translation="MQTLLNLFASVALLVWGTHIVRTGILRVFGASLRRMLSKSVSGR
                     ASAFVAGLGVTSLVQSSSATALIASSFVSQDLIALAPALAIMLGADVGTSLMAQVFSL
                     DLSWLSPLAIFFGV"
BASE COUNT          638 a         1529 c         1471 g          719 t
ORIGIN      
        1 gctcgcccag cttcaccagc aggcgaccga gcatttcggc ctccaggtcc cggtcggtgc
       61 cgcttccgcg cggctgccag cgcgtcgcat cctggttgat cgaggtgaag acctcctcga
      121 ggccgcggtg gctcacgtag atttcggtcg tcccgggctc cacgccgcgc tcgaggcggg
      181 tgcggaagcg gtcgcgctcg ccggtggagt acatgccggg aagcgcgcgc ccgatgatgc
      241 cgcgaatgcc cgtcgtctcg acgttcgcac gcgtgtcgcg ccagttggtc tcgatgattc
      301 cggcctcggg ggtctcgcgg gcgatcgaaa agccgatgtc ctgccagaaa tcgcggacga
      361 tcggccacac ctgcgcggga tcggccttcg cactgatcca gcgctggtcg ccgccgcgtt
      421 cgatatgcgc gttcggcacc gcgggcagga cccccgaggc ggacgacgaa accggggtgc
      481 cgggtgcgac ggcgctccgg tcgcggttgt actgcgagaa actggtcgag gcacgcggat
      541 cgggcaccgc gaagcgctcg tcggcggtgg gcgtggtcag gtccggcggc atttcgagtg
      601 gccgctcgcg ggagcgcgcc gtgcgctgct gttcctcggt cttgaagatt ccgcagccgg
      661 aagccaggac gagcgggacg agaacgagga cgagcgtgcg gatgcgtttc atggatgggg
      721 gattccggtt caggagaggc agcctgcttc gcgcagcgcg ccggtcacgg tttcgtggaa
      781 ggcgggcgcg agcggggtga gcggcaggcg gatgccctcg ccgatgcgtc ccatgcgcgc
      841 gagcgcccat ttcacgggga tgggattggc ctcgacgaag agtttctggt gcagccccat
      901 gaggcgctgg ttgagggcga tggccttctt cgcctccccg gcgagggcgg ccgcgcacat
      961 ctcgtgcatg gcgcgcggcg ccacgttggc ggtgacggag atgtcgccgt gcgcacccag
     1021 cagcatgtaa gggagcgctg tcgcgtcgtc gccggaatag accatgaact gcgcaggctt
     1081 ccttcgcaag agatccgcgc cccggtcgag gttgctcgtg gcgtccttga tgcccacgat
     1141 ccccggcacc tgcgcgagac gaagcgtggt gtcggtggac atgtccgcga ccgtccgtcc
     1201 gggaacgttg tacaggatca tcgggagatc caccgactcc gcgatggcct tgaagtgccg
     1261 gtagagccct tcctgcgcgg gcttgttgta atacggcacc accgacagcg aatagttcgc
     1321 gccgacgtcc ctggcgaact tcgtgagctc gatcgcctcg cgcgtcgagt tgccgcccgt
     1381 gcccgcgacc accggaatgc ggcccgcgca gtgctccacg gccgtcctga tcaggaggca
     1441 gtgctcgtcc acgtcgaccg tgggcgattc tcccgtcgtg ccgaccacga cgacgccatc
     1501 cgtgccttcg gcgatgtgcc agtcgaggag ggacttgaag cgggggatgt cgagcgcgcc
     1561 gtccgcctgc atgggcgtga cgattgccac taagctgccg gtgagcatgg ttccgatggg
     1621 ggagtcggtc gaaagaccgc gattttaccg cattccttcc gctctacgtc ccccggactg
     1681 ccggatcttc ccggcgggcg cagatccgct ggatcgcggc cggccgcggc accgtcacga
     1741 ccaggtcctc gtaacccacg acgcgcaagc tcccgcgcac ggcctcgagc agctcgccgg
     1801 gggcgagaag gaaatccgga ttggagggct tgccgaagcg ctcgttgccg agcgcgaagg
     1861 tctcgtagac cagcacgccg cccgacgcaa gggattgcac gagaacgggc agcagcggcc
     1921 ggtgcaggta gttggtcacc acgatcgcat cgaagagccg gccggcgaac ggccatggcc
     1981 cggcctcgac atcggccccg acaagcgtca cgccccccgg cgggtcgggg aagagccccg
     2041 gatcacggtc cacggccgtc acctcgaatc cctgcgccgc gaggtaacgg gcatgccgcc
     2101 cggaaccgca ggcgagatcg agcacctcgc cgccggggcg gacgaggctg gcccagcggg
     2161 cgacccacgg cgagggcgga aggtcggcga gatgcgcggt catgggctta tccgtttgcc
     2221 gtgccaggca cgaactcgga acgcgacgcc gggtccaacg cctgcctcgc gccggggccg
     2281 gaaccgcaac gaaaccctga catgttcctc accatcgccc tctgcctcgc cctggtcgtc
     2341 gccaccaccc tggttcactt cgagatgctc acgcaactct ccgcgaggct gccgggcatc
     2401 gtcatcgcct cgcggctgaa gctcgtggtc gtgatgctca cgacgtttgc cacgcacgcc
     2461 atcgaaatag cgctctatgc ggttgccatc tggtcctgca tctggctggc cggggtcgga
     2521 gggcttcccg gcgcctcggg gtcgactttc gtcaactgct tctatttttc cgccgaaacg
     2581 tacacgtccc tcgggttcgg agacatcgtc ccgtcgggtt ccctgcgcct gctggcgggc
     2641 gtggagacgc tcaacgggct gctcctgatc ggatggtctt cctcctacat ctacctctcc
     2701 atggagcggt tctgggacgt ggcacctacg gccgcaccct gagcgcatcg cgcgatcagg
     2761 aaagcatcga cagcgggtcg tggaatgcat ggcccagcgc ttcggcgacc tcgcggtggg
     2821 tgaccttgcc ctcgcagaca ttgaggccgg ccatgagatg cgcgtccgcg gcaagcgcgc
     2881 gtttccagcc cagcccggcg agctggagcg tgaagggaag cgtggcgttg ttgagcgcgt
     2941 aggtggaggt gcgcggcacc gcgcccggca tgttcgccac gcagtagtgc accaccttgc
     3001 cgaccaggaa cgtgggctcg tcgtgcgtgg tcgcgcgcga ggtctcgaag cagccgccct
     3061 ggtcgatggc gacgtcgatc acgaccgcgc cgggcttcat ctcgccgacg agcgcgcgcg
     3121 acaccagctt cggcgccgcc gcgcccggga tcagcacgcc gccgatcacc atgtcggcgg
     3181 agcacacgtg gtgctcgacg ctgtcgcggt tggaaaagac ggtgatcacg cgcgcgccga
     3241 gctggcgatc gatgcggcgc aacgcatcgg tattgcggtc gatcacgacc acgaccgcgc
     3301 cggtgcccac ggccatctgc gcggcgttcg agcccaccac gcccgcgccg aggatcacga
     3361 tcttcgcagg cggcacgccc gccacgccgc ccggcaggat ccccatcccg ccttgcgcct
     3421 tctccaggca gtgcgcgccg gcgtgcacgg ccatgcgccc ggcaacttcg gacatgggcg
     3481 cgagcaacgg caaccccccg cccgcctggg taaccgtctc gtaggcgatg cagaccgcgc
     3541 cgcttgcaag caggccctgg cactgctcgg ggtccggcgc gaggtgcagg taggtgaaga
     3601 ggatctgtcc cggccgcagc agggcgatct ccgcggcctg cggttccttc accttcacga
     3661 tcatgtcggc cgcggcgaag acttcctccg cgctcccgac gatgcgtgcg cccgccttct
     3721 cgtacgcgcc ctccgccgcg ccgatgccgg cacccgcccc ccgctcgacg agcacctcgt
     3781 ggccgcgttc caccacctcg cgcaccgacg agggcgtgag ccccacgcgg tattcctgga
     3841 ccttgatctc cttcggcacc ccgacacgca tttgcgcctc cccgattccg cgcggctcga
     3901 agggagcccg tccgggccat tatcgccgcg cttccccgcg cgcgaccagc gtcgtgccgc
     3961 gccgtcgcag cgaagctgtc ataattcaag gcagcgagtc cgactgaatt gcagacgctc
     4021 ctcaaccttt tcgccagcgt tgcgctcctc gtgtggggca cccacatcgt ccgcacggga
     4081 atcctgcgcg tcttcggcgc gagcctgcgg cgcatgctgt cgaagagcgt ctccggtcgc
     4141 gcctcggcct tcgtcgccgg actgggcgtc accagcctcg tgcagtcctc gagcgccacg
     4201 gcgctcatcg ccagttcctt cgtctcgcag gacctcatcg cgctcgcccc cgcgctcgcc
     4261 atcatgctgg gggccgacgt cggcacctcg ctcatggcgc aggtgttctc gctggacctt
     4321 tcctggctct cgccgctcgc catcttcttc ggcgtcg
//
LOCUS       DASUEV010000165         4883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1416301, whole genome shotgun sequence.
ACCESSION   DASUEV010000165 DASUEV010000000
VERSION     DASUEV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416301"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1094)
                     /locus_tag="VFV55_05355"
     CDS             complement(123..1094)
                     /locus_tag="VFV55_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX4943759.1"
                     /translation="MKKIEIDDRDRQMLELLAKGVSNRSIADSLGYREGTMRVYLHGL
                     YRKLGVGNKTSAVIWYFDRLKGDGGPGGGPDAPLAGAPMGESFGGMALRTSPYAALGA
                     MSMFLGAYGRMWEVAHRLKGAPLDEKADHRRRRSRLLWEAMLKGDFAYGKQLYDDDLT
                     ARLLVDSPSDCVLLGCLLRIGGYTKAADRVMAQLVKRRKGRVGISTREITLLTALRDV
                     VDNHTANGIASLYRLATESPAKPVPRHAAMAALYWIHRAGKDADAARATANAIWAEAE
                     AARQQLQAMGERPLDPAANVPPLATTRARIPGSRTGKATRARAELVE"
     gene            complement(1094..2869)
                     /locus_tag="VFV55_05360"
     CDS             complement(1094..2869)
                     /locus_tag="VFV55_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4943760.1"
                     /translation="MNVTPGTRPESPSPDLLRRLESRLPQLDRQELAGALAPLMARLQ
                     ERLSATESTAVTDAVLSLCRRLYADARSGDALPLAQGLLAQAGIAGDVVLERRASSAC
                     GLLAADTADVVGAIAYHARSLAIAAGQEDCVEQSRTWNNIGMAFGIAGNHELAARCYQ
                     RALALVQSVAGPVYSRCTASSNLANSLYQLGEVAEGLRFANLSLEQLSPAFLEQDVYG
                     AMLLRRNLVRLLISSDRVAEAEVHVQEAALLADRVRTPRAYVASAITRAAYELATGRI
                     DIALTRLDDALSKARSVPATLRDTLASIIRAEEAAGNAERALVRFHELADHVYRLAIA
                     RAHEHVELACLWDASATRADHLQEQARARLVSHLRPPGEPDEWKALQRLAVAAALRFD
                     NTGWHGVRVGELTKALAIASGLPPLQALEIGLAAELHDIGLVSVPEGILSKRGPLNNV
                     ERALVERHSEAGAEILRDDRHPRILLARDIAKYHHARWDGKGRPERVGGRFIPLPARL
                     CTVADAYDAMVCGLGREGAMTMSDALKELRREAGAQFDPELVACFEAMIRDETSDRGI
                     DPATMAGLENFQELVLSLQEDRGYV"
     gene            3024..4784
                     /locus_tag="VFV55_05365"
     CDS             3024..4784
                     /locus_tag="VFV55_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4943761.1"
                     /translation="MTAHLADPDRNPSFTKGIFLGELREDLLFPFPELTPQDKENLNA
                     ILGSFRAWASATVDSPRLDRDGRFGDEVRAGMAELGMMGLNIPEEYGGFGASALLFSR
                     VFGEVGTTDASLAVYFGAHQSIGCKGITLFGTDDQKRRWLTQCATGERVAAFCLTEAG
                     SGSDAQAMRTTATPSADGTHYLLNGEKIWISNAGYAGVLTVFAKVPVEVDGKAKQRVT
                     AFIVDARAPGITLGKIEEKMGIKASDTRSVSFENVRVPVEDRLGEVGSGFRIALEILN
                     SGRLGLSAGSSRGTRRVLDLALAYAKERTQFGRPIGSFEMIQRKFALAAADCYAADAG
                     WMTCAAMVDRGGIDFSLETAACKVFASELAFRAGCDAQQIAGGLGYSKEYPYEQAVRD
                     SRIMLIFEGTNEILRALIALSGLQQPGEQLKELGKAFRDPLRSLGAIGSYIGGRVKRQ
                     LVKPAFTRVHEALGDEAEMVAKAVHDLARAVEKLLIEHGKDVIERQFHQERLANAAID
                     IFLSTATLSRATWAIGKAGGVDKAADDLDNARIFISMALRRTRRALRALEHNQDARLK
                     GLAERALESGTLTVTTPTDA"
     gene            4781..>4883
                     /locus_tag="VFV55_05370"
     CDS             4781..>4883
                     /locus_tag="VFV55_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX4943762.1"
                     /translation="MTSIVISEERGVRHLHFGSTWVQGAMRIARPWSL"
BASE COUNT          747 a         1702 c         1669 g          765 t
ORIGIN      
        1 gggaactggt cctggtggcg ggtggcgatg ccaccccctg ctggccgtag ccgaccggaa
       61 accgtcggat cgcggccggc gcggctcgcc gcgccgccgc gctccgcgtc gaacccgggg
      121 cgctattcca ccagttccgc ccgggctctc gtggccttgc ccgtgcgtga acccggaatc
      181 ctggcccggg tagtcgcgag aggcggcacg ttcgccgccg ggtcgagcgg gcgctctccc
      241 atggcctgca gctgctgccg cgcggcctcc gcctcggccc agatggcgtt ggccgttgcg
      301 cgggcagcat cggcatcctt gccggcccgg tggatccagt acagcgccgc catcgcggcg
      361 tgccggggaa ccggtttcgc cgggctttcc gtcgcgagcc ggtacaggct cgcaatcccg
      421 ttggcggtgt ggttgtccac gacgtcgcga agcgccgtga gcagggtgat ttccctcgtc
      481 gagatgccga cgcggccctt ccttctcttg acgagctggg ccatgacgcg gtcggccgct
      541 ttcgtgtagc ccccgatccg caggaggcag ccgagcagca cgcaatccga cggcgagtcg
      601 acgagcaggc gcgccgtcag gtcgtcgtcg tagagctgct tgccgtaggc gaaatcaccc
      661 ttgagcatcg cttcccagag caggcgcgat cgcctgcggc gatggtcggc cttttcgtcc
      721 agcggggcac ccttgagcct gtgggcgact tcccacatcc ggccgtaggc tcccaggaac
      781 atgctcatcg cgcccagggc ggcgtaggga ctcgtgcgca gggccatgcc accgaaggac
      841 tcgcccatgg gcgctcccgc gagcggcgcg tccggacctc ccccgggtcc gccatcgccc
      901 ttgaggcggt cgaagtacca gatgaccgcg ctcgtcttgt tcccgacccc gagcttgcga
      961 tacaggccgt gcaggtaaac gcgcatcgtg ccttcccggt accccaggct gtcggcaatg
     1021 gacctgttcg aaaccccctt ggccagcagt tccagcatct gccggtcgcg atcgtcgatc
     1081 tctatttttt tcatcagacg taacccctgt cctcttggag ggaaaggacg agctcctgga
     1141 agttctccag gcccgccatg gttgccgggt cgatgccgcg atcggacgtt tcgtcgcgga
     1201 tcatcgcctc gaagcaggcc acgagctccg ggtcgaattg cgccccggcc tcgcgccgca
     1261 gttccttcag tgcgtcgctc atggtcatcg cgccctctcg cccgagtccg caaaccatcg
     1321 cgtcgtacgc atcggccacc gtgcacagcc gcgccggcaa gggaatgaac cggccgccca
     1381 cgcgctccgg acggcccttg ccgtcccagc gcgcgtggtg gtatttcgcg atgtcccgcg
     1441 ccaggaggat gcgcgggtgg cgatcgtcgc gcaggatctc cgcgccggcc tcggaatgcc
     1501 gttcgaccag ggcacgctcg acattgttga gcggaccgcg cttcgacagg atgccctcgg
     1561 gcaccgagac gagcccgatg tcgtgaagct cggctgcgag cccgatttcc agcgcctgga
     1621 gcggcggcag gcccgaggcg atcgcgagcg ccttggtcag ctcgccgacg cgcacgccgt
     1681 gccacccggt gttgtcgaag cgcaatgccg ccgcgacggc cagccgctgc agcgccttcc
     1741 actcgtcggg ctcaccgggc gggcgcaggt gcgacaccag ccttgcacgc gcctgttcct
     1801 gcaggtggtc ggcacgcgtg gccgacgcat cccagaggca ggcgagctcg acgtgttcgt
     1861 gtgcgcgcgc gatcgcgagg cggtagacgt ggtccgccag ttcgtggaag cgcaccaggg
     1921 cgcgctcggc gtttcccgcc gcctcctccg cccggatgat gctggccagc gtgtcgcgca
     1981 atgtcgccgg cacgctgcgg gccttcgaaa gcgcgtcgtc cagccgggtg agcgcgatgt
     2041 cgatgcggcc cgtggcgagt tcgtaggcag cgcgcgtgat cgccgatgcg acgtaggccc
     2101 ggggcgtgcg aacccggtcg gcgagcagcg cggcttcctg cacgtgcacc tcggcctccg
     2161 cgacccgatc cgaggaaatg agcaggcgca cgaggttgcg gcgaagcagc atcgctccgt
     2221 atacgtcctg ctcgaggaag gcgggcgaga gctgttccag cgacaggttc gcgaagcgca
     2281 ggccttcggc gacctcgccg agctggtaga gactgttcgc caggttcgac gaggccgtgc
     2341 accggctgta aacgggcccg gcaaccgact ggaccagtgc cagggcgcgc tggtagcagc
     2401 gggccgcgag ctcgtggttc cccgcgatgc cgaatgccat gccgatgttg ttccaggtgc
     2461 ggctttgctc gacgcaatcc tcctgccccg ccgcgatggc gagcgaacgc gcgtggtagg
     2521 cgatggcgcc cacgacatcc gctgtatcgg cggccaggag cccgcaggcg ctggacgcac
     2581 ggcgctcgag caccacatcc ccggcgatgc ccgcctgggc gagaaggccc tgcgcgagag
     2641 gcaatgcgtc gcccgaccgg gcatcggcat acaggcggcg gcacaacgac aggacggcat
     2701 cggtcaccgc cgtcgattcc gtcgcggaca gccgttcctg cagccgggcc atcaggggcg
     2761 caagggcccc ggcaagttcc tggcgatcga gttggggaag ccgggactcc aggcggcgga
     2821 ggagatccgg cgacggggat tcgggccggg tacccggcgt gacgttcacg taagcggcct
     2881 gattaccgtt aactgttaac tgtcctacgt ccgcattcta cgcatgcacg ccactgccgg
     2941 ataggggccg gtacccccgc tctccgccac cgggcaatcg cgctaaagtg acgcatcgcc
     3001 ggattcccgg cagatggacc aacatgaccg cgcaccttgc agaccccgac cgcaacccct
     3061 cgttcacgaa aggcatcttc ctcggcgaat tgcgcgagga cctcctcttc ccgttccctg
     3121 aactgacccc ccaggacaag gagaacctga acgccatcct cggctccttc cgcgcctggg
     3181 catcggccac ggtggactcc ccccgtctcg accgcgacgg gcgcttcggc gacgaagtcc
     3241 gcgccggcat ggccgaactg gggatgatgg ggctcaacat ccccgaggaa tacggcggct
     3301 tcggcgcctc agccctgctc ttcagccgcg tcttcgggga ggtgggaacg accgacgcgt
     3361 ccctcgccgt gtatttcggc gcccaccagt ccatcgggtg caagggaatc acgctcttcg
     3421 gcaccgacga ccagaagcgt cgctggctca cccaatgcgc cacgggcgag cgggtggccg
     3481 ccttctgcct caccgaggcg ggctcgggct ccgacgcgca ggccatgcgc actacggcca
     3541 cgcccagcgc ggacggcacg cattacctgc tcaatgggga aaagatatgg atctccaatg
     3601 ccggctatgc gggcgtgctg accgtcttcg ccaaggtgcc ggttgaagtg gacgggaagg
     3661 cgaagcagcg cgtcaccgcg ttcatcgtgg acgcgcgcgc ccccggcatc acgctcggca
     3721 agatcgagga gaagatgggc atcaaggcga gcgacacgcg cagcgtctcg ttcgagaacg
     3781 tgcgcgttcc cgtcgaggac cgcctgggcg aggtgggaag cgggttccgg atcgcgctcg
     3841 agatcctcaa ctccgggcgg ctgggccttt ccgcgggatc gtcgcgcggc acgcgcaggg
     3901 ttctcgacct cgccctcgcc tacgccaagg agcgcacgca gttcggccgg ccgatcggct
     3961 ccttcgagat gatccagcgc aagttcgccc tggccgccgc cgactgctac gcggcggacg
     4021 ccggctggat gacctgcgcc gcgatggtgg accgcggcgg catcgacttc tcgctggaga
     4081 cggcggcgtg caaggtgttc gcctccgagc tcgccttccg cgcggggtgc gatgcgcagc
     4141 agatcgccgg cggcctcggc tactcgaagg agtatccgta cgagcaggcg gtgcgcgatt
     4201 cgcggatcat gctcatcttc gagggaacga acgagatcct gcgcgcgctc atcgcgcttt
     4261 ccgggctgca gcagccgggc gagcagctga aggaactggg caaggcgttc cgggatccgc
     4321 ttcgctccct gggcgccatc ggttcctaca tcggcgggcg ggtgaagcgg cagctcgtga
     4381 agccggcctt cacgcgcgtg cacgaggcgc tgggcgacga ggcggagatg gtggccaagg
     4441 cggtccacga cctggcgcgc gccgtcgaga agctgctcat cgagcacggc aaggacgtga
     4501 tcgagcgcca gttccaccag gagcgcctgg ccaacgcggc catcgacatt ttcctctcga
     4561 ccgcgaccct ttcccgcgcc acctgggcca tcgggaaggc gggcggcgtg gacaaggccg
     4621 cggacgacct ggacaacgcg cgcatcttca tctcgatggc gttgcgccgc acgcgtcggg
     4681 cgctgcgcgc gctggaacac aaccaggacg cgcggctcaa ggggcttgcc gagcgagcgc
     4741 tcgaaagcgg caccctcacc gtcaccacgc cgacggacgc gtgacctcca tcgtcatcag
     4801 cgaggagcgc ggcgttcgcc acctgcattt cggatcgacc tgggtgcagg gcgcgatgcg
     4861 aatcgcgcgc ccgtggtcac tcg
//
LOCUS       DASUEV010000166         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1285993, whole genome shotgun sequence.
ACCESSION   DASUEV010000166 DASUEV010000000
VERSION     DASUEV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285993"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFV55_05375"
     CDS             <1..1077
                     /locus_tag="VFV55_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEX4943763.1"
                     /translation="RDELLGLPVADRDWVVVGATPAEMAARGFRAVGSDFPVFLHPDT
                     HEEYALARTERKTAPGYKGFVFHASPEVTLEEDLLRRDLTINAIARGEDGVLVDPHGG
                     IEDLRRGILRHVSEAFAEDPVRILRVARFAARFGFAVAPETLQLMRRMVHSGEADHLV
                     PERVWQEVARGLQESRPARMIEVLRECGALERVLPEVESLATGDGATLLAARLEASVP
                     FALCVRFACLTVGLAPEAARALCERINAPGECRDLAGVAARERAAVEGAGTLRADALL
                     ALLERTDAFRRPERLERLMEVCACDLRARGLARQVPRERIHAARVAALEVDAASIARD
                     HPQSVAAAIHAARAARIAEALASA"
     gene            complement(1166..1726)
                     /locus_tag="VFV55_05380"
     CDS             complement(1166..1726)
                     /locus_tag="VFV55_05380"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX4943764.1"
                     /translation="MFKVDQFSTANEAAINQFAHFAQLSLANLEKFAEIGLDAARDSV
                     EQATAHAAALAGAKDVHEVIAINSAALEPVMKRAYAYSRTVYETAAETNAEVKKVFEK
                     QAAEANKSAVAGLEEAFKYAPAGTETYVDNVKSAIAATQSAYANLAAINKQLYDTVEK
                     SVEQNVATVQSAAKAPKAPVGKAKRR"
     gene            complement(1993..2961)
                     /locus_tag="VFV55_05385"
     CDS             complement(1993..2961)
                     /locus_tag="VFV55_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX4943765.1"
                     /translation="MKRFTVILAAAVAAFGLGTAGAQEKKVRISIGTGGTAGVYYPLG
                     GGLAAVLSKFVPGVDATAEVTGGSVANLQLIGGGKSELGFTMADSAWDAYNGLEKFKD
                     KKVGVRTLAVFYPNRMQVATLDGKGIEKVTDLKGKRVATGSPSSGTEVMSMRMLEAFG
                     LDPNKDVNRERLSLAESVSALKDGKVDALTWVGGVPTPGLTDLAATPGKKIKLLDHGD
                     AAEGMRKKYGPIYVKNKILANAYPGETRETTNVDVWNLLVVPESADENLIYQITKTLF
                     EKKDELVKVHKDAAFLSLDNQLTGGSPIPFHPGALKYFREKGMKVN"
     gene            3217..>4506
                     /locus_tag="VFV55_05390"
     CDS             3217..>4506
                     /locus_tag="VFV55_05390"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX4943766.1"
                     /translation="MSQVIGVPKEVFPGERRVATVPEVVEKLRKLGFGVLVESGAGDA
                     ANCADDTYVAAGAEIAPDAKTLWSKADIVFKVRAPNLEEVSLIREGTTLVSFIWPAQN
                     PPLMEALAARKATVLAIDALPRMLSRAQKMDALTSMAGISGYRAVIEAANAFGRFFSG
                     QITAAGKVPPAKVFVAGAGVAGLAAIGTAASLGAIVRANDTRAEVADQVKSLGGEFVK
                     VDYEEEGSGGGGYAKVMSEGFQKAQRDMYAKQASEVDIIITTALIPGKPAPKLITADM
                     VKSMKPGSVIVDMAGEQGGNCELTVPGEAVVRHGVTIIGYTDLPSRLAKQSSTLYATN
                     LFRLAEELCKTKDGLINVNMEDDAIRGLTVVKEGAITWPPPPPKIPAAAPAQPKPAAA
                     AATPAKKSGHGHGAGEPASGKSLAITFGVGALLFLLVG"
BASE COUNT          678 a         1512 c         1554 g          762 t
ORIGIN      
        1 cgcgacgaac tcctcggcct gcccgtggcc gaccgcgact gggtggtagt cggcgccacg
       61 cccgccgaaa tggcggcgcg gggattccgc gcggtgggat ccgatttccc ggtgttcctg
      121 catcccgata cgcacgagga gtacgcgctc gcccgcacgg agcgcaagac cgcgccgggc
      181 tacaagggct tcgtcttcca cgcctccccc gaggtgaccc tcgaggagga cctgctccgg
      241 cgcgacctca ccatcaatgc catcgcgcgt ggcgaggacg gcgtgctggt cgatccccac
      301 ggcgggatcg aggacctgcg gcgcggcatc cttcgccacg tgagcgaggc gttcgccgag
      361 gatcccgtcc ggatcctgcg cgtcgcccgg ttcgcggcgc gcttcggctt tgcggtggcc
      421 ccggagaccc tgcagctcat gcgccgcatg gtgcactcgg gcgaggccga tcacctggtg
      481 cccgagcgcg tctggcagga ggtcgcgcgc ggcctgcagg aatcgcggcc ggcgcggatg
      541 atcgaggtgc tgcgcgaatg cggggccttg gaacgggtgc tgccggaagt ggagtcgctg
      601 gcgacggggg acggcgcgac cctgctcgcg gcgcgcctcg aggcgtccgt cccgttcgcc
      661 ctttgcgtgc gcttcgcctg cctcacggtc gggctcgcgc ccgaagcagc cagggcgctc
      721 tgcgagcgga tcaatgctcc cggggagtgc cgggacctcg ccggcgttgc cgcccgcgag
      781 cgcgctgcgg tcgagggcgc cggcacgctc cgggcagacg ccctgctcgc actcctggag
      841 cgcaccgacg cgttccggcg ccccgagcgc ctcgagcgcc tcatggaggt ctgcgcgtgc
      901 gacctgcgcg cgcgcggcct tgcacgccag gtgccgcggg aaaggattca cgccgctcgc
      961 gttgccgccc tggaggtcga cgcggcctcc atcgcgcgcg atcatccgca atccgttgcc
     1021 gccgccatcc acgcggcgcg cgctgcgcgc atcgccgagg cgctcgcctc agcctgagtc
     1081 ggccattctt cgccaaaaaa aacccccggt cgaaaccggg ggctttcatt tcaggcttgc
     1141 cgcacgcccc tcgcggggcg ggtaattacc ggcgcttggc cttgccgacc ggggccttcg
     1201 gggccttggc ggcggactgg acggtcgcga cgttctgctc gaccgacttc tcgacggtgt
     1261 cgtagagctg cttgttgatc gcggcgaggt tcgcgtaggc gctttgcgtg gcggcgatcg
     1321 cggacttcac gttgtcgacg taggtctcgg taccggcggg cgcgtacttg aaggcttctt
     1381 cgaggccggc gacggcggac ttgttggctt ccgcggcctg cttctcgaac accttcttca
     1441 cttcggcgtt ggtttcggcg gcggtctcgt acacggtgcg cgagtacgcg taggcgcgct
     1501 tcatcaccgg ctcgagggcg gcggagttga tggcgatgac ttcgtgcacg tccttcgcgc
     1561 cggccagtgc cgcggcgtgc gcggtggcct gctcgacgga gtcgcgcgcg gcatcaaggc
     1621 cgatctcggc gaacttctcg aggttggcga gggaaagctg ggcgaaatgc gcgaactggt
     1681 tgatggcggc ttcgttggcg gtgctgaatt ggtcgacctt gaacatggat gtctccttga
     1741 ctgtgaacgc tgggctatcg gtttcggcag cggttatgct gcactgcaca ataacgaatt
     1801 ctaggccatc cagcagtgat gtcaaggggg ttttgcaaat atttattgca ctgcaacata
     1861 accggtggca gcgttgcaaa tcaaggactt gcgcctgccc cgccagggcc ggaaaaggca
     1921 ggggcccgct ggcgggcccc tggatgcgct gccggctccg ccccggaatg gcccgggcgg
     1981 cggaggtcgc ggtcagttca ccttcatccc cttctccctg aagtacttca gggcgcccgg
     2041 gtggaacggg atcggggagc cgcccgtgag ctggttgtcg agcgagagga acgcggcgtc
     2101 cttgtgcacc ttcaccagct cgtccttctt ctcgaacagg gtcttggtga tctggtagat
     2161 gaggttctcg tcggccgact ccggcaccac gaggaggttc cacacgtcca cgttcgtcgt
     2221 ctcgcgcgtc tcgcccgggt aggcgttggc gaggatcttg ttcttcacgt agatcgggcc
     2281 gtacttcttg cgcattccct cggccgcatc gccatggtcg aggagcttga tcttcttgcc
     2341 cggcgtggcc gcgaggtcgg tgagtcccgg cgtgggcacg ccaccgaccc aggtgagcgc
     2401 atcgaccttg ccatccttga gggcgctcac gctctcggcg agcgaaagcc gctcgcggtt
     2461 cacgtccttg ttcgggtcga ggccgaaggc ctcgagcatg cgcatcgaca tcacctcggt
     2521 gccggagctg ggcgagccgg tggcgacgcg cttgcccttg aggtcggtga ccttctcgat
     2581 gcccttcccg tccagcgtcg cgacctgcat gcgattggga tagaagaccg cgagcgtgcg
     2641 cacgccgacc ttcttgtcct tgaatttctc gaggccgttg taggcgtccc aggccgaatc
     2701 ggccatggtg aagcccagtt ccgactttcc gccaccgatg agctggaggt tcgccacgga
     2761 gccaccggtc acctcggccg tcgcgtccac gccaggcacg aacttggaca ggaccgccgc
     2821 aaggccgccg ccaagggggt agtacacccc ggcggtgccg ccggtgccga tcgagatgcg
     2881 gaccttcttt tcctgcgcgc cggccgtgcc gagcccgaag gccgcgaccg ccgcggccaa
     2941 tatcacggtg aatcgtttca tcgctcctcg ctcctcagtt ttggtggcgc ctcgttggtt
     3001 ttggtggcgc ctcgcttgcc ggggaccatg atatccgctt cgcggctcga tgtgcgccta
     3061 cgtccggagc ggaatcgtcc ggcacgagaa gcacacgaaa agcatcggtg cctcccggac
     3121 accgcgtcag gaaagagtgg cagggagaga tcaagtaaaa tctgtccggc ccgcagactg
     3181 cgggccctcg tgacggttcc cgaaaggaag aagcgcatgt cccaggtgat cggcgtcccc
     3241 aaggaagtgt ttccgggcga aaggcgcgtc gccaccgtgc cagaggtggt ggagaaactg
     3301 agaaagctgg gcttcggcgt cctcgtggag tccggggccg gcgacgcggc caactgcgcc
     3361 gacgacacct acgtggccgc cggcgccgag atcgcgcccg acgcgaagac gctctggtcg
     3421 aaggcagaca tcgtcttcaa ggtgcgcgcc ccgaacctgg aagaggtttc cctcatccgc
     3481 gagggtacga cgctcgtgag cttcatctgg cccgcgcaga atcccccgct catggaggcc
     3541 ctcgccgcgc gcaaggccac cgtgctcgcg atcgacgcgc tgccccgcat gctcagccgc
     3601 gcccagaaga tggacgcgct cacctcgatg gccggcatca gcggctaccg cgcggtgatc
     3661 gaggcggcga acgccttcgg ccgcttcttc agcgggcaga tcacggccgc ggggaaggtg
     3721 ccgccagcca aggtcttcgt cgcgggcgcc ggtgtcgcgg gcctcgcggc catcggcact
     3781 gccgcgagtc tcggcgccat cgtgcgcgcc aacgacacgc gcgccgaggt cgccgaccag
     3841 gtcaagtcgc tgggcggcga gttcgtcaag gtcgactacg aggaggaggg ttccggcggc
     3901 ggcggctacg ccaaggtgat gagcgagggc ttccagaagg cccagcgcga catgtacgcc
     3961 aagcaggcga gcgaggtcga catcatcatc acgaccgcgc tcattcccgg caagccggcc
     4021 cccaagctga tcacggccga catggtgaag agcatgaagc cgggcagcgt gatcgtcgac
     4081 atggcgggcg agcagggcgg caactgcgag ctcaccgtgc cgggcgaggc ggtggtgcgg
     4141 cacggggtga ccatcatcgg atacaccgac ctgcccagcc ggttggccaa gcagtcctcg
     4201 acgctgtacg cgaccaacct gttccgtctc gccgaggagc tctgcaagac caaggacggc
     4261 ctcatcaacg tcaacatgga ggacgacgcc atccgcggcc tcacggtcgt caaggaaggc
     4321 gccatcacct ggccgccgcc gccacccaag atcccggccg cagcaccggc tcagcctaag
     4381 ccggccgcgg ctgcagccac gccggcaaag aagtcgggcc acggccatgg cgccggcgag
     4441 cccgcctcgg gcaagagcct cgcgatcacg ttcggcgtgg gcgcgctgct gttcctgctg
     4501 gttggt
//
LOCUS       DASUEV010000167        10909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_151920, whole genome shotgun sequence.
ACCESSION   DASUEV010000167 DASUEV010000000
VERSION     DASUEV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10909
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151920"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFV55_05395"
     CDS             <1..211
                     /locus_tag="VFV55_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX4943767.1"
                     /translation="DVLGTDRTLAIAPGDGVAILSAGAYGMVMSSNYNSRPRPCEVMV
                     DGARAVEVRPREPLASLYASESTLD"
     gene            complement(414..857)
                     /locus_tag="VFV55_05400"
     CDS             complement(414..857)
                     /locus_tag="VFV55_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943768.1"
                     /translation="MISGVMTGVTAGAMGFAGAAFFIAGFLAAFFFAGFFLAFLAGFF
                     AAFFFLAAGFFFATFFFAVFFAAFFFAAGFFFAAGFLFAAGFFVTFFFAAGFLAAFFF
                     AAGFFFAVAIVLLLSIVGPNCLSVAGGSFAVGPVTTKRSDFSIER"
     gene            complement(1025..1546)
                     /gene="thpR"
                     /locus_tag="VFV55_05405"
     CDS             complement(1025..1546)
                     /gene="thpR"
                     /locus_tag="VFV55_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX4943769.1"
                     /translation="MARLFFALWPDAGSQAALADWAREIANRGGGRPVSAARLHITLV
                     FLGEVDPARIAALCAAAEGVRSGAFTLGLDRIGAFRKTGVAWAGCRQPPAELLSLQAG
                     LERRIAAAGFSPDIRPFAPHLTLARRVRDPAGPADVEAVSWRVKAFALVESARGGGGY
                     RTLAEWPLEEGKT"
     gene            complement(1552..3003)
                     /gene="mgtE"
                     /locus_tag="VFV55_05410"
     CDS             complement(1552..3003)
                     /gene="mgtE"
                     /locus_tag="VFV55_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX4943770.1"
                     /translation="MPLSSDKPRSPTPQEHLDAVSKLLAKQRLVEEFAHRQGENDAKR
                     PGIVESLVHKQNLAELQKRLDGLHPADVAFILEGLPLEERLVVWGLVHSERDGEILLE
                     VSDGVRDTLLADMDSAEIVAAAQNLDADEIADLAPDLPHDVVQDIIEAQDVHDRAQLQ
                     SALSYPEGSVGALMDFEVVSIREDVTCEVALRYLRRFDELPSQTDALFVVDRDDHLRG
                     ILPLKRLLVSDPEVEVASLFDRDVVSFSPDSDAEEAAKAFERYDLVSAPVVSADGRVI
                     ARLPVDAVLDFVREKQESQELAKVGLREEEDLFSSVWLSAKNRGPWLAVNLCTAFFAS
                     RVVGAFEGSIEKLAALAALMPIVAGIGGNSGNQTTTLIVRALALDQVSAANARRLLAK
                     ELGISVLNGALWGGVLGIVAWLLYRNVALGGVLALAMMLNLVVAALAGLFIPLAMERF
                     GRDPAIGSSVFLTFVTDSMGFFIFLGLATIFLV"
     gene            complement(3100..3795)
                     /gene="mtgA"
                     /locus_tag="VFV55_05415"
     CDS             complement(3100..3795)
                     /gene="mtgA"
                     /locus_tag="VFV55_05415"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX4943771.1"
                     /translation="MNRLARLAGGAAIALAAAFVVYELSFLARLAWWRDHDPASTAFM
                     EDRLARAREKKPAAKLRHQWVAYERISGNLKRAIVAAEDAKFADHEGFDWEAMQKAWE
                     KNQKRGKVVSGGSTISQQLAKNLFLTGTRTPWRKAQEAMITVMMENIMDKRRILEIYL
                     NMIEWGDGVFGAEAAARHYFGVSAAALTPMQAARLAAMVPNPRFYDRNRGTPWLEKKT
                     EIILARMGSADVP"
     gene            complement(3792..4598)
                     /gene="aroE"
                     /locus_tag="VFV55_05420"
     CDS             complement(3792..4598)
                     /gene="aroE"
                     /locus_tag="VFV55_05420"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX4943772.1"
                     /translation="MDRYVIIGNPVAHSRSPEIHARFAAATGEAIAYERLLVTPGDFA
                     RATGEFFAAGGCGANVTLPFKVDAYAFANERTPRAGEAGAVNTLAQRGAAILGDNTDG
                     AGLVRDLCVNLGLELAGRRILLVGAGGAARGVVAPLLALAPTVLVIANRTADRATELA
                     QRFRHSGPVKGAGLDAMGEGYDLVVNATSSSTRGETLVLPGGLFAPGATAYDMAYGDP
                     ARPFLERARAAGARACDGLGMLVEQAAESFVLWRGKRPETASVIAALRAA"
     gene            4929..6338
                     /gene="glnA"
                     /locus_tag="VFV55_05425"
     CDS             4929..6338
                     /gene="glnA"
                     /locus_tag="VFV55_05425"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX4943773.1"
                     /translation="MAAADVFKMIKENEVKFVDLRFTDTKGKEQHVSVPVKAFGEDKF
                     TDGHAFDGSSIAGWKGIEASDMILMPDPDSVRMDPFMDEPTLALSCDVLEPLTGKGYE
                     RDPRSIARKAEAYLKSSGLGDTAYFGPEPEFFVFDAVEWKVDMSGSFVKIYSEEAPWS
                     SDAKLEGGNLGHRPPVKGGYFPVPPVDSLQDIRSAMCLALEEQGVEVEVHHHEVAAPG
                     QCEIGTKFSTLVKRADWLQILKYTVWNVARSYGKTATFMPKPVVGDNGSGMHVHQSVW
                     KGGQNLFAGDGYAGLSEFALYYIGGVIKHAKALNAITNPGTNSYKRLVPGFEAPINLA
                     YSARNRSASCRIPFVSSPKGRRVEVRFPDPTANPYLCFAALLMAGLDGVQNKIHPGDP
                     LDKNLYDLEPEEAAKVPHPCASLDEALVSLAKDREFLTRGGVFTNDMIDAYIALKYEE
                     VQRFRMTTHPVEFDMYYSA"
     gene            6538..7125
                     /locus_tag="VFV55_05430"
     CDS             6538..7125
                     /locus_tag="VFV55_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4943774.1"
                     /translation="MKTLWIGVAASLVSPFAFAQTTIYKHVDESGRVTYTNRPMKGAI
                     VLDLEPLSTLPAFAPATNPSAKPAARAPDKTTPKPSVAVVTPRGLPTAVASRDHDAQR
                     GRDELRRKTLEDELAKAEKSFDEARKALLDERRNPTLIAAVRAAQQADDPTPAQQAEM
                     RANIDRASGRIRGLQATVAEHEKNIEALKKELGAL"
     gene            7229..8293
                     /gene="glnL"
                     /locus_tag="VFV55_05435"
     CDS             7229..8293
                     /gene="glnL"
                     /locus_tag="VFV55_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX4943775.1"
                     /translation="MTSSFPGLELLATAVLLLDDHLHVTYANPAAETLLAHGRKHLLG
                     TALAKVMPGNDRFVAQLAQAVADDAGFNENELALELGGQATTVHCVAVPVEEDGSRLL
                     IELRELDQQIRIAKEAKLLEQQQANRELIRNLAHEIKNPLGGIRGAAQLLERELPDPE
                     LREFTQVIVKETDRLQSLMNRMLTPHRLPQIEMLNVHDVMERVRTLLLAEYPDGLVLR
                     RDYDVSLPDLFGDKESLIQAILNVARNAAQAMGGRGEIRFRTRIARQVTIARTRYRHA
                     VSVEIEDNGPGIPEDLAGKIFFPLVSGREGGTGLGLSLAQNYVHQHHGLIEFQSAPGR
                     TCFTILLPLRSPEQAGLTRH"
     gene            8311..9708
                     /gene="ntrC"
                     /locus_tag="VFV55_05440"
     CDS             8311..9708
                     /gene="ntrC"
                     /locus_tag="VFV55_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX4943776.1"
                     /translation="MNPVWIIDDDRSIRWVFEKALAREGIGHRAFATAQEALDCLAEE
                     PPQVVVSDIRMPGLSGLAFMQTLKERLPTTPVIVMTAYSDLESAVAAFQGGAFEYLPK
                     PFDVDHAVELVRRALDESRRRFEAPPADGDTPEILGQAAAMQEVFRAIGRLSQSNATV
                     LITGESGTGKELVARALHRHSPRADKAFIAINTAAIPKDLLESELFGHERGAFTGAQA
                     MRRGRFEQAESGTLFLDEIGDMPPDLQTRLLRVLSDGQFYRVGGHTPITANVRVIAAT
                     HQDLEARVRQGLFREDLFHRLNVIRLRLPSLRERRDDIPVLARHFLAKSARELGVEGK
                     RLTDAAVQFLSTREFPGNVRQLENLCHWVTVMAPGQVVDVADLPPEIRGEVPSASAGD
                     DGDWRRALEREVASRLARGETGIAGALTRDFETALITRALAHTGGRRIEAASLLGIGR
                     NTLTRKVQELQIDSK"
     gene            complement(9725..>10909)
                     /locus_tag="VFV55_05445"
     CDS             complement(9725..>10909)
                     /locus_tag="VFV55_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4943777.1"
                     /translation="LDRFKTINDSLGHDMGDELLKRVAARLTACVRASDTVARLGGDE
                     FAVILENLRDTDDEGAQQVADKMMEAMAAPMLVGNQHLNTSCSIGISLYPADGKDSAT
                     LMKHADVAMYYAKEKGRNNYQFFSAEMNLRAQERLSVENYLRLALRRNELVLHYQPRM
                     RMSDGALTGVEALLRWSHPRRGLLAPGKFIDVAEDSGLIVPIGEWVLERACRQARDWQ
                     RLLGKDFRLSVNLSVGQMADGDRLCRAVESALRGAGIEPSTLELELTESILMQNLGEK
                     AALLNRLGESGVGISIDDFGTGYSSLSYLKKLPVDAIKIDSSFVQDVGRDANDEAIVR
                     AIIAMAHSLRLSVIAEGVETDAQFACLKALGCDEYQGYHASPALPPAEFERRYAGKPA
                     RR"
BASE COUNT         1899 a         3755 c         3574 g         1681 t
ORIGIN      
        1 cgacgtgctc ggcaccgacc gcacgctcgc catcgccccc ggcgacggcg tcgccatcct
       61 gtcggcgggc gcctacggca tggtgatgag ctccaactac aactcgcgcc cgcgcccctg
      121 cgaggtgatg gtcgacggcg cgcgggcggt cgaggttcgc cctcgcgagc cgctcgcctc
      181 cctttacgcc agcgaaagca cgctcgactg agcgtcggcg cggatggccg gaaaatcgcc
      241 ccctcgatag tatcgagccg atactactgc ggcacgagtt cgatcaaata gttgcgcttg
      301 acacgccccc aaaccgcggt tgcgctgatt ttttcgcata tcatcagttc caccaagctg
      361 gtgcatcgaa gacacgaaag cggcgcgacg catcggaata gagccaccag gcttcaccgt
      421 tcgatcgaga aatccgagcg cttggtagtg acggggccca cggcgaagct gccgcccgcc
      481 acggagagac aattcggtcc aacaatcgaa aggagcaata cgatggcaac cgcgaagaag
      541 aaacctgctg cgaagaagaa ggccgccaag aagccggccg cgaagaaaaa agtaacgaag
      601 aagcctgcgg cgaagaggaa gcccgctgcg aagaagaagc ccgctgcgaa gaagaaagcg
      661 gcgaaaaaga ccgcgaagaa gaaggtagcg aagaagaagc ccgcggccaa gaagaagaag
      721 gctgcgaaga agccggccaa gaaagccaag aagaagccag cgaaaaagaa ggcggcaagg
      781 aaacctgcga tgaagaaggc ggcccccgcg aagcccatgg ctccggcggt tacccccgtc
      841 atcacgccgc tgatcaagcc gatgagctga acggaagccc cggcttccgg gccctacggc
      901 ctgaacccga atacgaggcg cgccccggcg cgacggctgc gaaagcggcc ggatacgcaa
      961 cccggggagc gcctcgcaaa cgggtgatcg attgaggaag gccttgcgcc gcccggcgcg
     1021 gccttcatgt cttgccctct tccagcggcc attcggccag cgttcgatag cctcccccgc
     1081 cgcgcgccga ttcgaccagc gcgaacgcct tcacgcgcca cgacaccgct tcgacatccg
     1141 cgggccctgc cgggtcgcgc acgcgtcgcg ccagcgtcag atgcggcgcg aacgggcgta
     1201 tgtcagggga aaagcccgcc gccgcgattc tccgttccag cccggcctgc agcgagagca
     1261 gttccgccgg gggctggcgg catcccgccc acgcaacgcc ggtcttgcgg aacgcaccga
     1321 tgcggtcgag ccccagcgtg aaggcgccgc ttcgcacccc ttccgccgca gcgcacagcg
     1381 ccgcgatgcg cgccgggtcg acctccccca ggaagacgag cgtgatgtgc agcctggccg
     1441 cggaaacggg ccggccgccg ccgcgattcg cgatctccct tgcccagtcg gcgagcgccg
     1501 cctgcgaccc ggcatcgggc cagagggcga agaagagccg cgccatggcc gctagaccag
     1561 gaagatcgtg gcgagcccga ggaagatgaa gaaccccatg ctgtccgtca cgaacgtgag
     1621 gaacacgctc gagccgatgg cggggtcgcg cccgaatcgc tccatcgcga gcgggatgaa
     1681 cagcccggcg agggcggcga cgaccaggtt gagcatcatc gcaagcgcca gcacgccccc
     1741 cagggccacg ttgcggtaca ggagccatgc gacgatgccc aggacaccgc cccacagcgc
     1801 gccattgagc acgctgatcc cgagttcctt cgcgaggagg cgccgggcat tggccgcgct
     1861 cacctggtcg agggccagcg cacgcacgat gagagtcgtg gtctggtttc ccgaattgcc
     1921 gccgatcccg gccacgatcg gcatcagggc ggccagcgcc gcgagcttct cgatcgagcc
     1981 ctcgaaggcg cccacgacac gcgaggcgaa gaacgccgtg cacaggttga cggcgagcca
     2041 cggcccgcgg ttcttggccg acagccagac cgacgagaag aggtcctcct cctcgcgcaa
     2101 gcccaccttc gcgagctcct gcgactcctg cttctcgcgc acgaaatcga gaaccgcgtc
     2161 cacggggagc cgcgcgatga cccggccgtc ggcggagacc acgggcgcgc tcaccaggtc
     2221 gtagcgctcg aaggcttttg cggcctcctc ggcatcgctg tcgggggaga aggagacgac
     2281 gtcgcgatcg aataggctgg ccacctcgac ttccgggtcg ctcaccagca ggcgcttgag
     2341 gggaaggatg ccgcgcaggt ggtcgtcgcg atcgaccacg aagagggcat cggtctggct
     2401 gggcagctcg tcgaagcgcc gcaggtagcg cagcgccacc tcgcaggtca cgtcctcccg
     2461 gatgctcacc acctcgaagt ccatgagggc gccgacgctg ccctcgggat aggacagggc
     2521 cgactgcagc tgggcgcggt cgtggacgtc ctgtgcctcg atgatgtcct gcaccacgtc
     2581 gtggggcagg tccggggcga ggtccgcgat ctcgtcggcg tcgaggttct gggcggcggc
     2641 gacgatctcc gccgagtcca tgtccgcgag aagcgtgtcg cggacgccat cggagacctc
     2701 cagcaggatc tcgccgtccc gctcgctgtg caccagaccc cagacaacga ggcgctcctc
     2761 gaggggaagg ccctcgagga tgaacgcgac atcggccggg tgcaggccgt cgagccgctt
     2821 ctgcaactcg gcaaggttct gcttgtgcac cagcgactcg acgattcccg gccgcttcgc
     2881 gtcgttctcg ccttgccggt gcgcgaactc ctccacgagc cgctgcttgg cgagcagctt
     2941 cgacacggcg tcgaggtgtt cctgcggcgt gggggagcgc ggtttgtcgg aggaaagcgg
     3001 catgggagat cgatcacttg cccgggcggg tggcgggtat tctaggctgc gggcccgaat
     3061 gccagccgcc ccgggagggg attattttcg gctcaccgct cagggtacgt ccgccgagcc
     3121 catgcgcgcg aggatgatct cggtcttctt ctccagccat ggcgtgccgc ggttccggtc
     3181 gtagaagcgg gggttgggaa ccatcgcggc cagtcgcgcg gcctgcatgg gcgtgagggc
     3241 ggccgcgctc acgccgaagt agtggcgcgc cgcagcctcg gccccgaaca cgccgtcgcc
     3301 ccactcgatc atgttgaggt agatctcgag gatgcgtcgc ttgtccatga tgttttccat
     3361 catgacggtg atcatcgctt cctgggcctt gcgccagggc gtgcgggtgc cggtgaggaa
     3421 aaggttcttc gccagctgct gcgagatcgt cgagccgccg gagaccacct tgccgcgctt
     3481 ctggttcttc tcccaggcct tctgcatcgc ctcccagtcg aagccctcgt gatcggcgaa
     3541 cttcgcgtct tccgcggcga cgatcgcacg cttgaggttg cccgagatgc gctcgtacgc
     3601 cacccactgg tggcgcagct tcgccgcggg cttcttctcg cgggcgcggg cgagccgatc
     3661 ctccatgaac gccgtcgacg cggggtcgtg gtcgcgccac cacgccagcc gcgccaggaa
     3721 ggaaagctcg tagacgacga atgccgcggc gagcgcgata gccgccccgc ccgcgaggcg
     3781 cgcgagccgg ttcacgcggc cctgagcgcg gcgatcacgc tcgccgtttc cggcctcttt
     3841 ccgcgccaca ggacgaagga ttccgccgcc tgctcgacga gcatgcccag gccgtcgcaa
     3901 gcccgtgcgc cggcggcgcg tgcgcgttcc aggaacggtc ttgccgggtc tccataggcc
     3961 atgtcgtagg ccgttgcacc gggcgcgaag aggccgcctg gcaaaaccag cgtttcaccg
     4021 cgggtcgacg aggacgtggc gttcaccacg agatcgtatc cctcgcccat cgcatcgagt
     4081 cccgctccct tgaccgggcc cgagtgccgg aaccgttgcg ccagttccgt cgcgcgatcg
     4141 gccgtccggt tcgcgatgac cagcacggtg ggcgcgagcg caagcagcgg cgccaccacc
     4201 ccgcgcgccg cgccgccggc gccgacgagc aggatgcgcc tgcccgcgag ctcgagcccc
     4261 aggttgacgc agaggtcgcg cacgagcccc gcgccgtccg tattgtcacc gaggattgcc
     4321 gcgccgcgct gcgccagcgt gttcaccgcg cccgcctccc cggcccgtgg cgtgcgttcg
     4381 ttggcgaatg cgtatgcgtc gaccttgaac ggcagcgtca cgtttgcgcc gcaaccgccg
     4441 gcagcgaaaa attcgcctgt cgctcgcgcg aaatcgcccg gcgtgacgag cagcctctcg
     4501 taggcgatcg cctcgcccgt cgcggctgcg aaacgcgcat gaatctcggg cgaacgcgaa
     4561 tgggcgacgg ggtttccgat gatgacgtag cgatccattg atcagggcaa ttcgaatgat
     4621 gcaggcgaag gatggacctt gcgccccgcg ccatgatagc cgcgggaggt cgttggccgc
     4681 gaatcgcaga tatagtgggc gcgggaaggc agcctccgcc tcccgttgcg cattcttggt
     4741 gcgcttgtat cattgatgca ccatgagagt gcgcttcttc gggcccatca ggtacaaatc
     4801 atgcaggttc aatgacttgc aggcataagt ccatggcatg atttctgctg cccaatgggc
     4861 gccaatcatt tatcaccccg atccattccc cattccgggg cgtcacccct caatcaggag
     4921 aaagagtcat ggcagcggcc gatgtcttca agatgatcaa ggaaaacgag gtcaagttcg
     4981 tggacctgcg cttcaccgac acgaaaggca aggagcagca cgtctcggtg ccggtgaagg
     5041 cattcggcga ggacaagttc acggacgggc acgcattcga cgggtcttcg attgcgggct
     5101 ggaagggcat cgaggcctcg gacatgattc tcatgcccga ccctgattcg gtgcgcatgg
     5161 atcccttcat ggacgagcct acgctcgccc tctcctgtga cgtgctcgag cccttgacgg
     5221 gcaagggcta cgaacgcgac ccgcgctcca tcgcaagaaa ggccgaggcc tacctcaaga
     5281 gctcggggct gggcgatacc gcctacttcg ggccggagcc cgaattcttc gtcttcgacg
     5341 ccgtcgagtg gaaggtcgac atgtcgggca gcttcgtcaa gatctattcc gaggaggccc
     5401 cctggtcttc cgatgcaaag ctcgagggcg gcaatctcgg gcatcgtccg ccggtgaagg
     5461 gcggctactt cccggttccg cccgtcgatt ccctccagga catccgctcg gccatgtgcc
     5521 tggccctcga ggaacaaggc gtcgaggtcg aggttcacca ccatgaagtg gcggcgcccg
     5581 gccaatgcga gatcggcacg aaattcagca cgctggtcaa gcgcgccgac tggctgcaga
     5641 tcctcaagta caccgtgtgg aacgtcgcgc gctcctatgg caagacggcg accttcatgc
     5701 ccaagcccgt cgtcggcgac aacggctcgg gcatgcacgt gcaccagtcg gtgtggaagg
     5761 gcggacagaa cctcttcgcg ggcgatggat acgcgggcct gtcggaattc gccctctact
     5821 acatcggcgg cgtgatcaag cacgcgaaag cgctcaacgc gatcaccaat ccgggcacca
     5881 actcgtacaa gagactcgtc cccggcttcg aggcgccgat caacctcgcc tactcggcgc
     5941 gcaatcgctc cgcgtcgtgc cgcatcccgt tcgtgtcgag cccgaagggg cgccgcgtcg
     6001 aggtgcgctt ccccgatccc accgcgaacc cgtatctgtg cttcgccgcg ctgctgatgg
     6061 ccggcctgga cggcgtgcag aacaagatcc accccggcga cccgctcgac aagaacctct
     6121 acgacctgga gccggaagaa gccgcgaagg tgccgcaccc ttgcgcgtcg ctcgacgagg
     6181 ccctggtgag cctcgccaag gatcgcgagt tcctcacccg cggcggcgta ttcaccaacg
     6241 acatgatcga tgcctacatc gcgctcaagt acgaggaggt gcagcgtttc cgcatgacca
     6301 cgcaccccgt cgagttcgac atgtactact cggcctgacg ccctgccgac ccatgacggc
     6361 gaaggggcgg cttcgttcgc ccctcgtcgg tttttccggc ctgtcccggg ccttgtggtg
     6421 ggcgccggcc caaaccacaa ggtatggggc attttctgcc tagcccgttg atctggttga
     6481 ctttttcaat gaccgctccc tatcctgatc ctcgactgtc gcgcgtggga gtgcgtgatg
     6541 aaaacgctgt ggatcggagt tgcggcaagc ctcgtctcgc cattcgcatt cgcgcagacg
     6601 acgatctaca agcacgtgga cgaaagcggc cgtgtcactt acacgaaccg gccgatgaag
     6661 ggtgcgatcg tgctcgacct cgagcccctg agcaccttgc cggccttcgc gcccgccacc
     6721 aatccgtcgg caaagccggc cgccagggcg ccggacaaga cgacgccgaa gccctccgtc
     6781 gccgtggtga cgccgagggg cctgcccacg gccgtggcct cgcgcgacca cgacgcgcag
     6841 agggggcgcg acgaacttcg ccgcaagacc ctcgaggacg agctggcgaa ggcggagaaa
     6901 tccttcgacg aggcgcgaaa ggccttgctg gacgagcggc ggaatccgac gctcatcgcc
     6961 gccgtgcgcg ccgcgcagca ggccgacgat ccaacgcctg cgcagcaggc cgagatgcgc
     7021 gcgaacatcg atcgcgcctc cggacgcatc cgggggctgc aggccacagt cgccgagcac
     7081 gagaagaata tcgaggccct caagaaagag ctcggcgcgc tttagccccg gcctcgcgca
     7141 gtttcccctt ccggctatcc ccttccgccg acgcgccagc gcgcttcgct ggcgcggccc
     7201 ttgcgcgttg cggccggaga gcgcgggcgt gacgtcatcg tttccgggcc tcgagttgct
     7261 cgccacggcc gtgctcctgc tcgacgatca cctgcacgtg acctacgcca accccgccgc
     7321 cgagacgctg ctcgcccacg ggcgaaagca cctcctgggc acggcgctgg ccaaggtcat
     7381 gccgggcaat gaccggttcg ttgcgcagct cgcgcaggcg gtggcggacg atgccggctt
     7441 caacgagaac gagcttgccc tcgagttggg cgggcaggcc actacggtgc attgcgtggc
     7501 cgttccggtg gaagaggacg ggtcgcgcct gctcatcgag ctgcgcgagc tcgaccagca
     7561 gatccgcatc gcgaaggaag ccaagctcct cgagcagcag caggccaaca gggaactcat
     7621 ccgcaacctc gcgcacgaga tcaagaaccc gctgggcggc atccgcgggg cggcgcagct
     7681 cctcgaacgc gagttgccgg accccgagct gcgggagttc acgcaggtga tcgtgaagga
     7741 aaccgaccgc ctgcagtcgc tcatgaaccg gatgctcacg ccgcatcgcc tgccgcagat
     7801 cgagatgctc aacgtgcacg acgtgatgga gcgcgtgagg accctgctgc tggccgagta
     7861 ccccgacggg ctcgtcctgc gccgcgacta cgacgtgagc ctgcccgacc tgttcggcga
     7921 caaggaaagc ctcatccagg caatcctcaa cgtggccagg aacgccgcgc aggcgatggg
     7981 gggccgcggc gagatccgct tccgcacccg catcgcgcgc caggtgacga tcgccaggac
     8041 ccgctaccgg cacgccgtat ccgtcgagat cgaggacaac ggccccggca ttcccgagga
     8101 cctcgcgggg aagatcttct tcccgctggt gagcgggcgc gagggaggca cggggctcgg
     8161 cctttcgctc gcgcagaatt acgtccacca gcaccacggg ctcatcgagt tccagagcgc
     8221 cccggggcgg acgtgcttca cgatcctcct gcccttgcgc agccccgagc aggccgggct
     8281 cacgcgccac tgacgatggg aggcgacgcc atgaaccccg tctggatcat cgacgacgac
     8341 cgctcgatcc gctgggtctt cgagaaggcg ctcgcccgcg agggcatcgg ccaccgcgcg
     8401 ttcgccaccg cccaggaagc gctcgactgc ctggcggagg aacccccgca ggtcgtggtg
     8461 agcgatatcc gcatgccggg gctgtcggga ctggcgttca tgcagacgtt gaaggagcgc
     8521 ctgccgacga cgccggtgat cgtcatgacg gcctattccg acctcgaaag cgccgttgcg
     8581 gccttccagg gcggcgcgtt cgagtacctg ccgaagccct tcgacgtcga ccacgccgtg
     8641 gaactggtgc gccgcgcgct cgacgaaagc cggcgccggt tcgaggcacc cccggccgac
     8701 ggcgatacgc ccgagatact cggacaggca gccgcgatgc aggaagtctt tcgcgccatc
     8761 ggacggctgt cgcagtcgaa cgcgaccgtc ctgatcaccg gcgagtcagg caccggcaag
     8821 gaactcgtgg cgcgcgcgct gcaccgccac agcccgcggg ccgacaaggc cttcatcgcc
     8881 atcaacaccg ccgcgatccc gaaggacctc ctcgaaagcg agctcttcgg ccacgaacgc
     8941 ggggcgttca ccggggcgca ggcgatgcgc cgcggacgct tcgaacaggc cgagtccggc
     9001 acgctgttcc tggacgagat cggcgacatg cccccggacc tgcagacgag gctcctgcgc
     9061 gtgctctccg acggccagtt ctaccgcgtc ggcggccaca cgccgatcac cgccaacgtg
     9121 cgcgtgatcg cggccaccca ccaggacctc gaggcgcgcg tgcggcaggg gctgttccgc
     9181 gaggatctct tccatcgcct gaacgtcatc cgcctgcgcc ttcccagcct tcgcgagcgc
     9241 cgcgacgaca tcccggtgct cgctcgccac ttcctggcga aaagcgcgcg cgagctgggc
     9301 gtggagggaa agcgcctcac cgacgcggcg gtccagttcc tctcgacgcg cgagtttccc
     9361 ggcaacgtgc gccagctcga gaatttgtgc cactgggtga ccgtcatggc ccccgggcag
     9421 gtcgtagacg tggcggacct tccgccggag atccgggggg aagtgccttc ggcatcggcg
     9481 ggcgacgacg gcgactggcg ccgagcgctc gaacgcgagg tcgcctcccg cctggcgcgc
     9541 ggcgagacgg gcatcgccgg cgctctcacg cgcgatttcg agaccgccct catcacccga
     9601 gcgctcgccc ataccggcgg gcgtcgcatc gaggctgcaa gcctgctcgg catcggccgc
     9661 aataccctta cgcgcaaggt gcaggaactt cagatcgatt cgaagtagcc ctcttccccg
     9721 atcctcatcg tcgcgcgggc tttcccgcat agcgcctctc gaactccgcc ggcggcagcg
     9781 cgggactcgc atggtatccc tggtattcgt cgcagcccag ggccttgagg caggcgaact
     9841 gggcgtcggt ctccacgccc tccgcgatca ccgatagccg cagcgaatgc gccatggcga
     9901 tgatggcgcg cacgatcgcc tcgtcgttcg catcgcgccc gacgtcctgc acgaacgagc
     9961 tgtcgatctt gatcgcgtcc accggcagtt tcttgaggta ggaaagcgac gagtagccgg
    10021 tgccgaagtc gtcgatggag atgcccacgc cgctctcgcc gaggcgattg aggagcgccg
    10081 ccttctcgcc gaggttctgc atgaggatgc tttcggtgag ctccagttcc agcgtggatg
    10141 gttcgatccc cgctccacgc agcgcgcttt cgaccgcccg gcagagccgg tcgccgtcgg
    10201 ccatctggcc cacggaaagg ttcaccgaga gcctgaagtc cttgccgagc aggcgctgcc
    10261 agtcgcgcgc ctgccggcag gcgcgctcga gaacccactc gccgatggga acgatgagcc
    10321 ccgagtcctc cgcgacgtcg atgaatttcc cgggagcgag cagcccgcga cgcgggtgcg
    10381 accagcgcag cagcgcctcc accccggtga gcgccccgtc ggacatgcgc atgcgcggct
    10441 ggtagtgcag caccagctcg ttcctgcgca gcgcaaggcg caggtagttc tcgacggaaa
    10501 gccgctcctg cgcgcgcagg ttcatctccg cggagaagaa ctggtagttg ttgcggccct
    10561 tctccttggc gtagtacatc gccacgtccg cgtgcttcat gagcgtggcg ctgtccttgc
    10621 cgtcggcggg gtagaggctg atgccgatcg agcacgaggt gttgaggtgc tggttgccga
    10681 cgagcatcgg cgcggccatc gcctccatca tcttgtcggc gacctgctgc gccccttcgt
    10741 cgtcggtgtc gcgaaggttc tcgaggatga ccgcgaactc gtcgcccccc agccgtgcca
    10801 cggtgtcgga ggcgcgcacg catgcggtga gccgcgccgc gactcgcttc aggagttcgt
    10861 cgcccatgtc gtggccgagg gaatcgttga tcgtcttgaa ccggtccag
//
LOCUS       DASUEV010000168         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_850136, whole genome shotgun sequence.
ACCESSION   DASUEV010000168 DASUEV010000000
VERSION     DASUEV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850136"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFV55_05450"
     CDS             complement(<1..365)
                     /locus_tag="VFV55_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX4943778.1"
                     /translation="MEEPTVPVPAPEIRHAELKAALVLALTVAIACGFVLYVLYARGT
                     FESTQRLVLMADDGAGVTVGMDLTFSGFPVGRVQRIDLASDGKARIEIDVPRKDSRWL
                     RASSVFTLERSLVGGARLRA"
     gene            complement(371..1129)
                     /locus_tag="VFV55_05455"
     CDS             complement(371..1129)
                     /locus_tag="VFV55_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4943779.1"
                     /translation="MLQSGAAGARRWLRLSAEALVATLSPSTYTAPVRALAARQVCSS
                     AWQILPGFVFVCALLSFLVIRIVVSTAHNYGLADLALDVFVRLLVLELIPLFVALFVA
                     LRSGTAIATEVALMRVRGELDEARRSEGADPLRTELLPRGIGTAAAVLALTAIGGLVA
                     LVMAYVGVYGLSPWGHAAYSRAIGQVFEPVVLAGLALKVVLFAFAVAVIPVAASLGAS
                     RDLRSVPEAAPRGLVLLFVVLALLEAASLAIKYS"
     gene            complement(1362..1745)
                     /gene="panD"
                     /locus_tag="VFV55_05460"
     CDS             complement(1362..1745)
                     /gene="panD"
                     /locus_tag="VFV55_05460"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX4943780.1"
                     /translation="MLRTMLKSKLHRVTCTGSELHYEGSCAIDQDLLDAADIGEFQQI
                     DVYNVNNGERFTTYAIAAQRGSGVISVNGAAAHKARRGDLLIIAAYATYTEAELAKYS
                     PDLVYVDANNAIRDKRRAIPVQKAA"
     gene            complement(1837..2667)
                     /gene="panC"
                     /locus_tag="VFV55_05465"
     CDS             complement(1837..2667)
                     /gene="panC"
                     /locus_tag="VFV55_05465"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX4943781.1"
                     /translation="MDLIHTVKELRERLKGEGSIVLVPTMGNLHAGHLALVEEARKHG
                     TCIVASIFVNRLQFEPGGDFDRYPRTLEHDCAALEKAGCHVVFAPDEKEVYPEAQEIL
                     LTPPRSAQQLCGDFRPGHFQGVLTVVAKLFDIVAPHTAVFGRKDYQQLFVIRALVRQM
                     NYPIAIVGVETRREPDGLAMSSRNGYLSSEERTGAVRLSQNLKRIKVLVESGRHDYEA
                     LCADATRDLTAAGWRVDYVAVRNQSGLARPEAGDRDLVVLGAAWLGRTRLIDNLEFRA
                     "
     gene            complement(2680..3471)
                     /gene="panB"
                     /locus_tag="VFV55_05470"
     CDS             complement(2680..3471)
                     /gene="panB"
                     /locus_tag="VFV55_05470"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX4943782.1"
                     /translation="MRVTTPKLQERARGGEKLAMLTCYDASFAAISDAAGVDILLIGD
                     SLGMVIQGHDSTLPVTMDETEYHVRAVARGSANALVLADMPFGSFQESREQAFRNAAR
                     LLAAGAQMVKLEGGATMVDTTRFLVERGIPVCAHIGLTPQSVHTLGGYRVQGRDDDTA
                     GRLVADAKALEAAGAAIVLMEAMPASVAKRVTESLAVPTIGIGAGPDVSGQVLVVYDM
                     LDIYPGRKARFVRNFMEGAPSVKAAIEAYVKAVKAKTFPGPEHCF"
     gene            3616..>3886
                     /locus_tag="VFV55_05475"
     CDS             3616..>3886
                     /locus_tag="VFV55_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX4943783.1"
                     /translation="MPAPRRLIADIGGTNARFALVGEHGAPACIRVLASRDYAHIADA
                     IEAYLAGTGMERPDEAAIAIATPVTGDNVRMTNLPWAFSARALKSR"
BASE COUNT          667 a         1347 c         1269 g          603 t
ORIGIN      
        1 gcgcgcaggc gcgcgccccc cacgaggctg cgctcgagcg tgaagacgct ggaggcgcgc
       61 agccagcgcg agtccttgcg cggcacgtcg atctcgatcc gcgccttgcc atcagacgcg
      121 aggtcgatgc gctgcacgcg ccccacggga aaaccggaaa aggtgaggtc catgccgacg
      181 gtcacgccgg ctccgtcgtc ggccatcagc accagccgct gcgtgctttc gaacgtgccg
      241 cgggcgtaca gcacatagag cacgaagccg cacgcgatcg ccacggtgag tgccagcacc
      301 agcgcggcct tcagctcggc gtgccggatc tcgggggcgg ggacggggac ggtcggctct
      361 tccacggggc tcaggagtac ttgatggcga gggaagcggc ctcgagcagc gcgaggacca
      421 cgaagagcag cacgagtccg cgcggcgcgg cctcgggcac cgagcgcaaa tcgcgcgacg
      481 ccccaaggct cgcggcgacg ggaatcaccg ccaccgcgaa ggcgaagagc acgaccttca
      541 gcgcaagccc cgccagcacc accggttcga acacctggcc gatggcgcgc gagtaggccg
      601 cgtgtcccca gggagacagg ccgtagacgc cgacataggc catcaccagc gcgaccaggc
      661 caccgatcgc ggtgagcgcg aggaccgcgg ccgcggtgcc gatcccccgc ggcagcaact
      721 cggtgcgcag cggatccgcg ccttcgctgc ggcgtgcctc gtcgagttcc ccgcgaaccc
      781 gcatcagcgc gacttcggtg gcgatggccg tccccgagcg cagcgcgacg aagagggcga
      841 cgaagagcgg aatgagctcg agcaccagca agcgcacgaa cacgtcgagc gccaggtccg
      901 ccagcccgta gttgtgggcg gtcgagacga cgatgcggat gacgagaaag ctgaggagcg
      961 cacagacgaa cacgaagccc gggaggatct gccaggcgga cgagcagacc tgccgcgccg
     1021 ccagggcccg caccggtgcc gtataggtgg atggcgacag cgttgcgacg agcgcctcgg
     1081 cgctcaagcg cagccagcgc cgcgcgcccg ccgcgcccga ttgcagcatc ccgtcggtcc
     1141 cggcgaccgc ccgccgaaat ggtgtggcct gcatgcgccg atcatacctc gctcggcgct
     1201 ctggaaaagg ggttcggtaa aaggcgggtt aaaggggtca ggttacttgg tttaaagggg
     1261 tcaggttact ttactgtcga cagaattcga ttgggctgta aagtaacctg acccctttaa
     1321 accaagtaac ctgacccctt taaccggacc ccttttcctg atcaggcagc tttctggacc
     1381 ggtatcgccc ggcgcttgtc cctgatggcg ttgttcgcgt ccacgtatac gaggtcgggg
     1441 ctgtacttgg cgagctccgc ctcggtatag gtcgcatagg cggcgatgat gagcagatcg
     1501 ccgcggcggg ccttgtgggc ggccgcgccg ttcaccgaga tgaccccgga gccgcgttgc
     1561 gccgcgatgg cgtaggtggt gaaacgctcg ccgttgttca cgttgtagac gtcgatctgc
     1621 tggaattcgc cgatgtccgc ggcgtccagg agatcctggt cgatggcgca cgagccctcg
     1681 taatggagct cggagcccgt gcaggtgact cgatggagct tcgacttgag catggtgcgt
     1741 agcatggtta cttctccgga atgacaactg aatcgagtct accgggcctt tttagagcaa
     1801 tggctcgaaa actccgcgcg gccccgggtt gtctcgtcac gcgcgaaatt cgaggttgtc
     1861 gatcagccgc gtccggccca gccaggccgc cccgaggacc acgagatcgc ggtccccagc
     1921 ctccggtcgg gccaatccgg actgattgcg cactgcaaca taatcgacgc gccagccggc
     1981 ggccgtcaag tcccgcgtgg catcggcgca aagcgcctcg taatcgtgcc gcccgctttc
     2041 caccaggacc ttgatccgct tgagattttg gctgaggcgc accgccccgg tgcgctcttc
     2101 agaggagaga tagccgttcc gggaggacat cgcgaggcca tcgggctcgc gcctggtctc
     2161 caccccgacg atcgcaatcg ggtagttcat ctgccgcacc agggcccgga tcacgaacag
     2221 ttgctggtag tccttgcgcc cgaaaacggc tgtgtgcggc gcaacaatat cgaagagctt
     2281 cgcgacgacc gtcagcacgc cctggaagtg ccccggacgg aaatcgccgc acagttgctg
     2341 cgcggatctg gggggcgtca gcaggatctc ctgcgcctcg ggatagacct ccttctcgtc
     2401 cggcgcgaac accacgtggc agcccgcctt ctcgagcgct gcgcaatcgt gctcgagcgt
     2461 gcgcgggtag cggtcgaaat ccccgcccgg ctcgaactgc aggcgattga cgaagatcga
     2521 ggcgacgatg cacgtgccgt gcttccgtgc ctcctcgacg agcgccaggt gccccgcatg
     2581 gaggttgccc atcgtcggca cgagaacgat cgacccctcg cccttcagcc tctcccgcaa
     2641 ctccttcacg gtatgaatca gatccacgcc gcatcactct cagaagcaat gctccggccc
     2701 cggaaatgtc ttcgccttca ccgccttcac gtaagcctcg atcgccgcct tcaccgaggg
     2761 cgcgccttcc atgaagttcc tcacgaagcg cgcctttcgc ccggggtaga tgtcgagcat
     2821 gtcgtagacc accagcacct gccccgacac gtccggcccc gcgcctatgc cgatggtggg
     2881 caccgcgagc gattccgtca cgcgcttcgc caccgaagcg ggcatcgcct ccatcagcac
     2941 gatggccgcc cccgccgcct ccagggcctt cgcgtccgcc accaggcgcc ccgccgtgtc
     3001 gtcgtcgcgg ccctgcacgc ggtagccgcc cagcgtgtgc accgactgcg gcgtgaggcc
     3061 gatgtgcgcg cagaccggga tgcctcgctc cacgaggaag cgcgtcgtgt ccaccatcgt
     3121 cgcgccgcct tccagcttca ccatctgggc gcccgcggcc aggagtcgcg cggcattgcg
     3181 gaaggcctgc tccctcgatt cctggaagct tccgaacggc atgtccgcga ggaccaacgc
     3241 attggcgctt ccgcgggcga cggcgcggac gtggtactcg gtctcgtcca tcgtcacggg
     3301 aagcgtcgaa tcgtgcccct ggatcaccat gcccagcgaa tcgccgatga gcaggatgtc
     3361 gacgccggcc gcgtccgaaa tggccgcgaa gctcgcgtcg tagcaggtga gcatggcaag
     3421 cttttcgccg cctcgcgcgc gttcctgaag cttcggtgtg gtcactctca tgggaatggc
     3481 aatgaacgca tggggacgag cattctgcca cggtttcgct gcaatgcagc atggcgccgg
     3541 ggcgcgccgc gcccgccggc ggacgactcg ctggtaggat tcggcgaccc gcccgcctcc
     3601 cttggagccc gtgacatgcc cgcccccagg cgcctgatcg ccgacatcgg cggcaccaac
     3661 gcgcgcttcg ccctggtggg cgaacacggc gcgccggcct gcatacgggt gctcgccagc
     3721 cgcgactacg cgcacatcgc ggatgccatc gaggcctacc tcgccgggac gggaatggag
     3781 cgccccgacg aggcggcgat cgccatcgcg accccggtga ccggcgacaa cgtgcgcatg
     3841 accaacctcc cctgggcctt ctcggcccgg gcgctcaagt cgaggc
//
LOCUS       DASUEV010000169        10082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1080705, whole genome shotgun sequence.
ACCESSION   DASUEV010000169 DASUEV010000000
VERSION     DASUEV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080705"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFV55_05480"
     CDS             complement(<1..1175)
                     /locus_tag="VFV55_05480"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX4943784.1"
                     /translation="MTERRKKPEELRSHRWYGVHDLRSFGHRSRTAQMGFHRSDYAGK
                     PVIAIVNTWSDINPCHTHFRQRAEEVKRGVWQAGGFPVEIPALTLSEPFQKPTTMLYR
                     NLLAMETEEMLRSYPVDGCVLMGGCDKTTPALVMGALSMNLPSIFVPAGPMLRGDFRG
                     GFLGSGSDTWKYWAELRAGNITEEDWQGVENGIARSPGHCMTMGTASTMTSAAEAMGL
                     TLTGAASIPAPDSRHAQMATLSGKRIVDMVWEDLKVRDIVDARAIDNAVTTVLALGGS
                     TNAIVHLIAIARRAGVALDLARFDALARKTPVLADIRPSGKYLMEDFFYAGGLPGLLT
                     RLDDLLDASARTVTGHSLGENLAGAAVYNDDVIRKRDNPLVGKDGLAVLTGNLAPRGA
                     "
     gene            complement(1259..2302)
                     /locus_tag="VFV55_05485"
     CDS             complement(1259..2302)
                     /locus_tag="VFV55_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4943785.1"
                     /translation="MTALPPSPPPRPRRARRGAGGLTLSDVAHIAGVSPITVSRALNT
                     PEQVSPEKLARVREAVARTGYVPNRVAGGLASSRSRLVAAIVPTIASPMFLDTVHALT
                     DTLAQEGYQVMLGESGYDGTREDALLDAIIGRRPDGVVLTGVMHSVEGRRRLAAAGIP
                     VVETWDLTPTPIDMLVGFSHEKAGSAAAEYLYERGARRPAMICADDQRAGLRQRGFCE
                     AAARLSLGKVPLRLVHAPTTIGDGRSALAALLERDPGPDAVYCSSDVLALGALIEARS
                     QGIRIPGELRIFGFGDLDFAAHADPALSTARIDGAAIGRQAARFLLERIAGGLEGTRI
                     VDLGFTIIPRDSA"
     gene            complement(2786..3829)
                     /locus_tag="VFV55_05490"
     CDS             complement(2786..3829)
                     /locus_tag="VFV55_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX4943786.1"
                     /translation="MQGKRFAAIAGALAVCVTSGALCAWLRTPLPWMIGPLAGMALFQ
                     FGGASLEAPPFGREAGQLTIGIALGLYFTPAVAREVLANGPTLFAAAAGVFLIGVAAS
                     LFLERAAGVDHPTAFFSSAIGGAAEMVILADRHDALVDRVALAHSLRLLVVVTAVPVA
                     ITLLGQTGSDDYRPVLVPFDPAKLALLAGLALASAFAWRRTGLPNPFMMGPLLLVIAL
                     TAGGVVFSSIPSWMTNVAQLLLACNLGSRFQQSFLREAPRFMAAVLASVALMLVLAAL
                     LALGLAWLAGLMPATILLACAPGGIAEMSITAKVLRVGVAFVTAAHVTRFVIVMLFTE
                     PAYRLLARRRAKG"
     gene            3960..4325
                     /locus_tag="VFV55_05495"
     CDS             3960..4325
                     /locus_tag="VFV55_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX4943787.1"
                     /translation="MNPYVVFLLYMGAILGVVAMMLMLNHMLGPKPKDTETKLEPFEC
                     GATPVDTHNVKAVPIKYYAVAIIFILFDLETVFLFIWALGAQPLTGFMLFTLGIFVFL
                     LVLILLYVYKARLLEAVRQ"
     gene            4328..4849
                     /gene="nuoB"
                     /locus_tag="VFV55_05500"
     CDS             4328..4849
                     /gene="nuoB"
                     /locus_tag="VFV55_05500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX4943788.1"
                     /translation="MNPRTIPIHPAGSTGKSAAFEYLSTRKDELVGWARKFSLFPYPF
                     VTACCAMEFMAVSSSPYDTDRFGAALPRFSPRQSDLLMVVGTVTHKQAPILLKVYEQM
                     CEPKWVMAFGVCATSGGFYQNYAALPGIDKIIPVDIYVPGCPPRPETVIDGIMRLQDK
                     IAREKHPIITKDF"
     gene            4855..5358
                     /locus_tag="VFV55_05505"
     CDS             4855..5358
                     /locus_tag="VFV55_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX4943789.1"
                     /translation="MGAEVFDSITARLAARQEEASSPGTARASTTVSHGVLVVDLAPS
                     AVEDAVRALKGEFAFDIFLDVTAIDWPGQDPRFEVVWHFYSTTHKVRVRLKARVPEAN
                     PVVVSLTSVYGSAAFMERECHDMYGIVFEGNADLRPILLYEGFVGHPLRKDYPKDREQ
                     PLVPYRE"
     gene            5383..6612
                     /gene="nuoD"
                     /locus_tag="VFV55_05510"
     CDS             5383..6612
                     /gene="nuoD"
                     /locus_tag="VFV55_05510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX4943790.1"
                     /translation="MNIPANPRIPNPVRSALAKSDRDDTVIVSIGPSHPATHGTIQIF
                     AELDGERIIRADVHCGYLHRGFEKECEAHTWHNLIPYVDRLNYCSPLINDFAYCDAVE
                     RLMGVEITPRTKYLRTLLSEYSRLCDHLTCVAAALMELGAMTAFLYLVMIRDYMYEHI
                     AALTGARVTHSYGRIGGLACDLPEGWLARLAEILDQYEEFIDRVHGLVDRNRIFIDRM
                     RDVGVLTTAQALDFGYTGPMLRSTGAARDLRKDTPYLAYAELDFEVPVGIKGDNYDRY
                     YVRMREMDESVHMIRQLVDMLPQGPINVDDRRCTFPEKTAVYGEIESLINHFKLVMEG
                     PRVPAGEIYHAHESANGELGFYLVSTGEGVPYKVHVRAPSFVHMCGANKLLEGYQLAD
                     LVPTFGSINMIGGECDR"
     gene            6609..7073
                     /gene="nuoE"
                     /locus_tag="VFV55_05515"
     CDS             6609..7073
                     /gene="nuoE"
                     /locus_tag="VFV55_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX4943791.1"
                     /translation="MKHLIPEFEKMKERYPAGFEASLVLPCLQRIQVDRGFVAEEDIV
                     ALVDYLKVPRIQVEEVLSYYTMLRRKPIGRWHLQACRNIACSMRGAEGILGHLSSRLG
                     IQPGQTTPDGRFTLTAVECLGSCGTAPVVMVNEAYHENMSAEKLDRLIEELR"
     gene            7076..8368
                     /gene="nuoF"
                     /locus_tag="VFV55_05520"
     CDS             7076..8368
                     /gene="nuoF"
                     /locus_tag="VFV55_05520"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX4943792.1"
                     /translation="MPGRQLIMDFEVTPTSHTLEAYRARGGYATIEKALKSMQPIDVT
                     KEVTASGLLGHGGAAFPVGRKWSVMKPGDGQPHYLCCNADEGEPGTFKDRWILENAPH
                     LLVESMLLASYALGVRNAFVYIRGEFDLPWRRIQGAIDEAYAAGLLGAPLLGTDFVLD
                     IVVYRGAGSYVCGEASGLITSIEGRKPYPRNRPPRLTVKGLYQCPTVINNVETLANVT
                     NIIRDGAEAFRKVGTAKSPGTALVSISGHIAKPGVYEVEYGYPWVKFLDEDCGGMLNG
                     AKLKCVVPGGISTKVLTRAEIEPLTLSHESVNPAGSGLGSGGMIAIAEGTCMVRLLQV
                     LLRFYHHESCGQCTPCREGMGWMHKIIDRIVDGKGVPEDIDRLYAISYANDGTTICGM
                     GDAAGYATIGILNKYRDEFEYYIMNKRSRCDGRLECPA"
     gene            8356..9798
                     /locus_tag="VFV55_05525"
     CDS             8356..9798
                     /locus_tag="VFV55_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX4943793.1"
                     /translation="MPRLTIDGREIEAAPGQTVMQAATAAGIFIPYFCWHPQLSVPGN
                     CRICVVGVEDKGGEWVEIACNMPVTEGMVVFTNSEKVRARRKATLQFITLNHPVDCGI
                     CDKAGECTLQDYHFTYNGERSISIDAKVHATKHYDLSSRIVLDNERCIQCTRCVRFTR
                     EISKSNGLGMQNRADHSLVRAVEDGSFDADPYSDNVIDICPVGALLSRSFLHKARVWY
                     LLPTPSVCPGCARGCTVNLWHRRPEWKLRSPAQRVRNDEIDRVTPLENPAVNGPWICN
                     KGRDLARIFGRARAEVSMLKGRPAELQGAIAQARSLIAAAKKPVAIVSSWGSNEELAA
                     CKAALGARFRSFVKADQQAEPGEVVEDAILIRADKNPNTAGARALFGDAAPEIPKDAD
                     LVLVWGEGFDFGRIPVGAKIVYLNAWLQPENGHADVFIPVSIQTERNGHYTNFAGVTS
                     AFSTCFPKQAGVADAEAIFAALAGTAGGGS"
     gene            9795..>10082
                     /locus_tag="VFV55_05530"
     CDS             9795..>10082
                     /locus_tag="VFV55_05530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX4943794.1"
                     /translation="MIADLVISLVFIGYAISVLMGFGTILTWVERKQAAVMSDRIGAN
                     RAYIRIPFTQVKLVWWGLFHGMADGLKMLLKEDFKPRTYDWYAYAVAPWVVF"
BASE COUNT         1696 a         3445 c         3417 g         1524 t
ORIGIN      
        1 gcgccgcggg gcgcgaggtt gccggtgagc acggcgagcc cgtccttccc gaccagcgga
       61 ttgtccctct tgcggatcac gtcgtcgttg tagacggccg cccccgcgag gttctcgccg
      121 aggctgtggc cggtcaccgt gcgcgcggac gcgtcgagga gatcatcaag ccgcgtaagc
      181 aggccgggaa gaccgccggc atagaagaag tcctccatca ggtacttgcc cgacgggcgg
      241 atgtcggcga gcaccggggt cttgcgcgcc agcgcgtcga agcgcgcgag gtccagggcc
      301 acgccggcgc ggcgcgcgat cgcgatgagg tgcacgatcg cgttggtcga gccccccagc
      361 gccaggaccg tggtgacggc attgtcgatg gcgcgcgcgt ccacgatgtc gcgcaccttc
      421 aggtcctccc acaccatgtc gacgatgcgt tttcccgaca gtgtggccat ctgcgcgtgg
      481 cgcgagtcgg gcgcggggat ggaggcggcg ccggtcagcg tgaggcccat ggcttcggcg
      541 gcactcgtca tcgtggaggc ggtgcccatg gtcatgcagt ggcccggcga gcgcgcgatg
      601 ccgttctcga caccctgcca gtcttcctcg gtgatgttgc cggcgcgcag ctccgcccag
      661 tacttccagg tgtcgctgcc gctgccgagg aacccgccgc gaaaatcgcc gcgcagcatc
      721 ggcccggcgg gaacgaagat cgacggcagg ttcatggaga gcgcgcccat cacgagcgcc
      781 ggcgtcgtct tgtcgcaccc gcccatcagc acgcagccgt cgaccggata ggagcgcagc
      841 atctcctcgg tttccatcgc gaggaggttg cggtagagca tcgtcgtggg cttctggaag
      901 ggctcggaga gcgtgagcgc gggaatctcc acggggaagc cgcccgcctg ccagacgccg
      961 cgcttcactt cctccgcgcg ctggcggaag tgcgtgtggc acggattgat gtcgctccag
     1021 gtgttgacga tggcgatgac gggcttgccc gcgtagtcgg agcgatggaa gcccatctgc
     1081 gcggtgcgcg agcgatgccc gaagctgcgc aggtcgtgca ccccgtacca gcggtggctg
     1141 cgcaactctt ccggcttctt gcggcgttcg gtcacttcga gagctccagg ggtggggcga
     1201 aaagttcgga tggtagcgct atcaacaaag gcggtcaatg gcggtacgga cggcagcctc
     1261 aggcgctgtc gcgcgggatg atcgtgaagc ccagatcgac gatgcgggtg ccctcgaggc
     1321 cacccgcgat gcgctcgagc aggaagcgcg cggcctgccg gccgatggcg gcgccgtcga
     1381 tccgcgcggt ggagagggcg gggtcggcgt gcgcagcgaa atcgagatcg ccgaacccga
     1441 agatgcgcag ctcgccgggt atccggatgc cctgggacct ggcttcgatc agggcgccga
     1501 gcgcgaggac gtcggaactg caatagacgg cgtccgggcc ggggtcgcgc tcgagaagcg
     1561 cggccagggc gctgcggcca tcgccgatgg tggtgggggc gtgcaccagg cgcagcggga
     1621 ctttccccag cgacagccgc gccgcggcct cgcaaaagcc tctttgccgc agcccggcgc
     1681 gctggtcgtc ggcgcagatc attgccggcc gccgtgcgcc cctttcgtac aggtactcgg
     1741 ccgccgccga tccggccttt tcgtgggaaa aaccgaccag catgtcgatg ggggtcgggg
     1801 tgaggtccca ggtctcgacc accgggatgc ccgcagccgc cagccgccgg cggccttcca
     1861 cggagtgcat gacgccggta agcaccacgc cgtccggtcg ccggccgatg atcgcgtcga
     1921 gcaaggcgtc ctcgcgcgtg ccgtcgtagc cgctctcgcc cagcatcacc tggtatccct
     1981 cctgggcgag ggtgtcagtg agcgcatgca ccgtatcgag gaacatcggg ctcgcgatgg
     2041 tcggcacgat cgccgcgacc agccggctgc ggctcgaggc gagcccgccg gcgaccctgt
     2101 tgggcacata cccggtgcgc gccacggcct cccggacccg ggccagcttc tccggcgaga
     2161 cctgttccgg cgtgttcagc gcgcgcgaaa cggtgatggg cgacacgccc gcgatatgcg
     2221 cgacgtccga cagcgtgagg cccccggcgc cccggcgggc gcggcgcggg cgtggtggcg
     2281 gggaaggtgg cagggcagtc atggccgtag cgcgagcatc gtcgatgata acgctaccag
     2341 ccggcaaacg ggaggccagg cggcaaaggg gcgggcggcg aaggggcggg cggcgaaggg
     2401 gcggacggtc aagtaggagg cgggcggtca cattctcgac ggagctcgcc gtcagagcgg
     2461 gcagccccct aatgcacggt ggtgaacgga gcccgcattt ggggtcgggt tggttgggtt
     2521 tcttttttgg tttttcggcc ggattgactt gccatcaact tcgacgctgc cggcgtatcg
     2581 acataggttc ggatcgtgac gccagcagca gattgacccg gccgaaaaac caaaaaagaa
     2641 acccaaccaa cccgacccca cccatcccct tcgatcaccg ccgcaccgag tggggacgca
     2701 accgatcgcg cgcattcacc gccgtgccga atccggacga gaccgatcgc cttccgtcgc
     2761 cgccgtgccg aatcggccca accccctagc ccttcgcccg ccgcctcgcc aggagccggt
     2821 aggccggctc ggtgaagagc atcacgatca cgaagcgcgt cacgtgtgcc gccgtgacga
     2881 aggccacgcc cacgcgcaac accttcgctg tgatcgacat ctcggcgatg ccgcccggcg
     2941 cgcaggcgag gaggatcgtg gcgggcatca ggcccgcgag ccaggccagc ccgagggcca
     3001 ggagcgcggc gaggaccagc atgagggcca cggaggccag caccgccgcc atgaagcggg
     3061 gtgcctcgcg caggaagctc tgctggaagc gcgagccgag gttgcacgcg agaagcagct
     3121 gcgccacgtt tgtcatccac gacggtatcg aggagaacac cacgccgccc gcggtgagcg
     3181 cgatcacgag caggagcggg cccatcatga acgggttggg tagcccggtg cgccgccagg
     3241 cgaacgcgga tgcgagcgcg aggccggcga ggagggcgag cttcgccggg tcgaagggca
     3301 ccaggacggg ccggtagtcg tccgagcccg tctggccgag cagcgtgatg gccacgggca
     3361 ccgcggtcac gacgacgagc agccgcagcg aatgcgccag cgcgacgcga tccacgagcg
     3421 cgtcgtgccg gtccgcgagg atcaccatct ccgcggcgcc gccgatggcg ctcgagaaga
     3481 aggccgtggg gtgatccacg cccgccgcgc gctccaggaa gaggctcgcg gcgacgccga
     3541 tcaggaacac gcccgcggcg gcggcgaaga gcgtgggccc gttggcgagc acctcccgcg
     3601 caaccgcggg cgtgaaatag aggcccagcg cgatgccgat ggtgagctgg ccggcttcgc
     3661 gcccgaaggg aggtgcctcc aggctcgcgc cgccgaactg gaagagcgcc atgcccgcca
     3721 gcggcccgat catccagggc agcggcgtgc gcagccaggc gcacagcgcg ccgctcgtga
     3781 cgcacaccgc gagcgcgccc gcgatcgccg caaatcgctt cccttgcatg ggccgcattg
     3841 tgcccggggc aaatgccacg gtcacggtcg ggttgatgtg cgtcaaacgg cgggttggac
     3901 taaaggctaa tctgcccgtg gcaggaaacc cctcgcgggc tccgccgcaa gaacagacca
     3961 tgaatcccta tgtagtcttc ctcctgtata tgggcgcaat cctcggcgtc gtcgcgatga
     4021 tgctgatgct gaaccatatg ctcgggccca agccgaagga caccgagacc aagctcgagc
     4081 ccttcgaatg cggcgccacg cccgtcgaca cccataacgt gaaggcggtg ccgatcaagt
     4141 actacgcggt ggccatcatc ttcatcctgt tcgacctgga gacggtcttc ctgttcatct
     4201 gggcgctcgg cgcgcagccg ctcacgggct tcatgctctt cacgctgggg atcttcgtgt
     4261 tcctgctggt gctgatactc ctttatgtgt acaaggcgcg attgctcgag gccgtgcggc
     4321 aatgacgatg aatccgcgca ccatccccat ccatccggcg ggctccacgg gcaagtccgc
     4381 ggccttcgag tacctctcga cgcgcaagga cgagctcgtg ggctgggcgc gcaagttctc
     4441 gctgttcccc tatccgttcg tcaccgcctg ctgcgccatg gaattcatgg cggtgagctc
     4501 cagcccctac gacaccgacc gcttcggggc ggcgctgccg cgcttctcgc cgcgccagtc
     4561 ggatctcctg atggtggtgg gcacggtcac gcacaagcag gcgccgattc tgctcaaggt
     4621 gtacgagcag atgtgcgagc ccaagtgggt gatggcgttc ggcgtgtgcg cgacgagcgg
     4681 cggcttctac cagaactacg cggcgcttcc cggcatcgac aagatcatcc ccgtcgacat
     4741 ctacgtcccc gggtgcccgc cgcgccccga gacggtgatc gacggaatca tgcgactcca
     4801 ggacaagatc gcacgcgaga agcacccgat catcaccaag gatttctgag aacgatgggc
     4861 gccgaggttt tcgacagcat caccgctcgc cttgcggcaa ggcaggagga ggcatcatcg
     4921 ccggggacgg cccgtgcctc gaccacggtt tcgcacggcg ttctcgtcgt ggacctggcg
     4981 ccgtccgcgg tcgaggacgc cgttcgcgcc ctcaagggcg agttcgcctt cgacatcttc
     5041 ctcgacgtga cggccatcga ctggccgggg caggacccgc gcttcgaggt ggtgtggcac
     5101 ttctactcga ccacgcacaa ggtgcgcgtg cgcctgaagg cgcgcgtgcc cgaggcgaat
     5161 ccggtcgtcg tctcccttac gtccgtgtac ggctcggccg cgttcatgga gcgcgagtgc
     5221 cacgacatgt acggcatcgt cttcgagggc aacgcggacc tgcgcccgat cctcctttac
     5281 gaaggcttcg tcgggcaccc gctgcgcaag gactacccga aggaccgcga gcagccgctc
     5341 gtgccgtacc gggaatagcg tggtcgacga acgagccggc agatgaacat tcccgccaat
     5401 ccccggatac ccaatcccgt ccgctccgcg ctcgcgaaga gcgaccgcga cgacacggtg
     5461 atcgtgagca tcggcccgtc gcacccggcc acgcacggca cgatccagat cttcgcggaa
     5521 ctcgacggcg agcgcatcat ccgcgccgac gtgcactgcg gctacctgca tcgcggcttc
     5581 gagaaggaat gcgaggcgca cacctggcac aacctcatcc cgtacgtcga ccgcctgaac
     5641 tactgctcgc cgctcatcaa cgacttcgcc tactgcgacg ccgtcgagcg gctcatgggc
     5701 gtggagatca cgccgcgcac gaaatacctg cgcacgctgc tctcggaata ctcgcggctg
     5761 tgcgaccacc tgacgtgcgt cgcggcggcg ctgatggagc tgggggcgat gaccgccttc
     5821 ctctacctgg tgatgatccg cgattacatg tacgagcaca tcgcggcgct gaccggcgcg
     5881 cgcgtgacgc attcgtacgg gcggatcggc ggcctcgcct gcgacctgcc ggaagggtgg
     5941 ctcgcgcgcc tcgccgagat cctcgaccag tacgaggaat tcatcgaccg cgtgcacggg
     6001 ctggtggacc gcaaccgcat cttcatcgac cgcatgcgcg acgtgggcgt gctcacgacg
     6061 gcgcaggcgc tcgacttcgg ctataccggc ccgatgctgc gctcgacggg tgccgcgcgg
     6121 gacctgcgca aggacacgcc ctacctcgcc tacgccgagc tcgacttcga ggtgccggtg
     6181 ggcatcaagg gcgacaacta cgaccgctat tacgtgcgca tgcgcgagat ggacgaatcg
     6241 gtgcacatga tccggcagct cgtggacatg ctgccgcagg gccccatcaa cgtggacgac
     6301 cggcgctgca ccttccccga gaagaccgcg gtgtacggcg agatcgagtc cctcatcaac
     6361 cacttcaagc tggtgatgga gggcccgcgc gtgccggccg gcgagatcta ccacgcccac
     6421 gagagcgcca acggggagct cggcttctac ctggtttcca ccggcgaggg cgtgccgtac
     6481 aaggtgcacg tgcgcgcccc cagcttcgtg cacatgtgcg gcgccaacaa gctgctcgag
     6541 ggctaccagc tcgccgacct cgttcccacc ttcggctcga tcaacatgat cggcggggag
     6601 tgcgaccggt gaagcacctc atccccgagt tcgagaagat gaaggagcgc taccccgcgg
     6661 ggttcgaggc ctcgctcgtg ctgccgtgcc tgcagcgcat ccaggtcgac cgcggcttcg
     6721 tggccgagga ggacatcgtc gccctcgtgg actacctcaa ggtgccgcgc atccaggtcg
     6781 aggaggtgct ctcgtactac acgatgctgc gcaggaagcc catcggccgc tggcacctgc
     6841 aggcctgccg caacatcgcc tgctccatgc gcggcgccga gggcatcctc gggcacctct
     6901 cgtcccggct cgggatccag cccggccaga ccacgcccga tggccgcttc acgctcacgg
     6961 cggtggagtg cctcggctcc tgcggcaccg cgcccgtggt gatggtgaac gaggcctacc
     7021 acgagaacat gagcgcggaa aagctcgacc ggctgatcga ggagctgcgc tagccatgcc
     7081 cggacgccag ctcatcatgg atttcgaggt gacgcccacc tcgcacacgc tcgaggccta
     7141 ccgcgcgcgc ggcggctacg ccacgatcga gaaggcgctc aagtcgatgc agccgatcga
     7201 cgtcacgaag gaagtcaccg cctcgggcct gctgggccac gggggcgccg ccttcccggt
     7261 cgggcgcaag tggtcggtga tgaagcccgg cgacggccag ccgcactacc tgtgctgcaa
     7321 cgccgacgag ggcgagccgg gaaccttcaa ggaccgctgg atcctggaga acgcgccgca
     7381 cctgctcgtg gaatcgatgc tcctcgcttc ctacgcgctg ggcgtgagga acgcgttcgt
     7441 ctacatccgc ggcgagttcg acctgccgtg gcgccgcatc cagggcgcca tcgacgaggc
     7501 ctacgcggcc gggctcctgg gcgccccgct cctgggaacg gacttcgtcc tcgacatcgt
     7561 ggtctaccgc ggcgccggct cctacgtctg cggcgaggcc tccgggctga tcacctcgat
     7621 cgagggcagg aagccctacc cgaggaaccg cccgccgcgc ctcaccgtga agggcctcta
     7681 ccagtgcccg acggtgatca acaacgtcga gacgctggcc aacgtcacca acatcatccg
     7741 cgacggcgcc gaggccttcc gcaaggtcgg caccgccaag agccccggca cggcgctcgt
     7801 atcgatctcc gggcacatcg cgaagcccgg cgtctacgag gtggagtacg gctacccctg
     7861 ggtcaagttc ctcgacgagg actgcggcgg catgctgaac ggggcgaagc tgaagtgcgt
     7921 cgtccccggc ggcatctcga ccaaggtgct cacgcgcgcc gagatcgagc cgctcacgct
     7981 ctcccacgag tcggtgaacc ccgccggttc cggactgggc tccggcggca tgatcgccat
     8041 cgccgaggga acgtgcatgg tgcgcctgct gcaggtgctg ctgcgcttct accaccacga
     8101 gtcctgcgga cagtgcacgc cgtgccgcga gggcatgggg tggatgcaca agatcatcga
     8161 ccgcatcgtg gacgggaagg gcgtgccgga ggacatcgac cgcctctacg cgatttccta
     8221 cgccaacgac ggcaccacca tctgcggcat gggcgacgcg gcgggctacg ccacgatcgg
     8281 catcctcaac aagtaccgcg acgagttcga gtactacatc atgaacaagc gctcgcgctg
     8341 cgacggaagg ctcgaatgcc ccgcctgacc atagacggcc gcgagatcga ggcggcgccc
     8401 ggccagaccg tcatgcaggc ggccacggcc gcgggaatct tcatcccgta cttctgctgg
     8461 cacccgcagc tctcggtgcc cggcaactgc cgcatctgcg tggtgggcgt cgaggacaag
     8521 ggtggcgagt gggtggaaat cgcctgcaac atgccggtga ccgagggcat ggtggtgttc
     8581 accaattcgg agaaggtgcg cgcgcggcgc aaggccacgc tgcagttcat cacgctcaac
     8641 catcccgtgg actgcggcat ctgcgacaag gccggcgagt gcacgctgca ggactaccac
     8701 ttcacgtaca acggcgagcg ctcgatctcc atcgacgcga aggtccacgc caccaagcac
     8761 tacgacctgt ccagccgcat cgtgctggac aacgagcgct gcatccagtg cacgcgctgc
     8821 gtgcgcttca cgcgcgagat ctcgaaatcg aacggcctcg gcatgcagaa ccgtgccgac
     8881 cactcgctgg ttcgcgccgt ggaggacggg agcttcgacg ccgaccccta ctccgacaac
     8941 gtgatcgaca tctgcccggt gggcgcgctc ctgtcgcgct ccttcctgca caaggcgcgc
     9001 gtctggtacc tgctgcccac gccctcggtg tgcccgggtt gcgcgcgcgg ctgcaccgtg
     9061 aacctctggc accgccggcc ggagtggaag ctgcgctcgc ccgcgcagcg cgtgcgcaac
     9121 gacgagatcg atcgcgtcac gccgctggaa aacccggcgg tgaacggtcc gtggatctgc
     9181 aacaagggcc gcgacctcgc caggatcttc ggccgcgcgc gcgccgaagt gtcgatgctc
     9241 aaaggccggc cggcggagct gcagggcgcc atcgcccagg cgcggtccct cattgccgcg
     9301 gcgaagaagc ccgtggccat cgtctcgagc tggggctcca acgaggagct cgccgcgtgc
     9361 aaggcggccc tgggcgcgcg ctttcgttca ttcgtgaaag cggaccagca ggcggagccg
     9421 ggcgaggtgg tcgaggacgc gatcctcatc cgcgccgaca agaaccccaa caccgccggc
     9481 gcgcgcgcgc ttttcggcga cgcggcgccc gagatcccga aggacgccga tctcgtcctc
     9541 gtctggggcg agggcttcga cttcggccgc atcccggtgg gggcgaagat cgtgtacctc
     9601 aatgcctggc tgcagccgga aaacggccac gccgacgtgt tcatcccggt cagcatccag
     9661 accgagcgca acgggcacta caccaacttc gcgggggtga cgagcgcctt ctcgacctgc
     9721 ttcccgaaac aggcgggcgt ggccgacgcc gaggcgatct tcgccgcgct cgccggcacg
     9781 gccggaggcg gatcatgatc gcggacctgg tcatctcgct ggtcttcatc ggctacgcga
     9841 tcagcgtgct gatggggttc ggcaccatcc tcacctgggt ggaacgcaaa caggcggccg
     9901 tgatgtcgga ccgcatcggc gccaaccgcg cctacatccg tatcccgttc acgcaggtga
     9961 agctggtctg gtggggactc ttccacggca tggccgacgg cctgaagatg ctgctcaagg
    10021 aggacttcaa gccgcgcacc tacgactggt acgcctacgc ggtggcgccc tgggtggtgt
    10081 tc
//
LOCUS       DASUEV010000170         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1081441, whole genome shotgun sequence.
ACCESSION   DASUEV010000170 DASUEV010000000
VERSION     DASUEV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081441"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFV55_05535"
     CDS             complement(<1..610)
                     /locus_tag="VFV55_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX4943795.1"
                     /translation="MKTAHVFLMCPPEYFDVSYIINPWMQGNVRRIDNALAKQQWRAL
                     YDILTDHATVRLIASQPGSPDMVFTANAGLVKGRRFLASRFRYPERQYEEPYFADWFL
                     DRGYDVSLMPRDVPFEGAGDALFDRGAGRLWLAHGHRSAAAAKGEIARRFGGEVIALR
                     LTDQRFYHLDTCLCPLEGGHLLYYPAAFDAPSRAAIERRVPAA"
     gene            complement(688..1866)
                     /locus_tag="VFV55_05540"
     CDS             complement(688..1866)
                     /locus_tag="VFV55_05540"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX4943796.1"
                     /translation="MTDEYGPDTLGVRAGGLRSDFREHSEALVLTVSFVFKSAAEAAR
                     RFANEEPGNIYSRFTNPTVAMFQSRLAALEGAQACVATSTGMAAVATMAMGLLSAGDH
                     MIAARGVFGTVVPLLNQILGRFGVETTWVDATDLDAWRSALRPRTKLLFAETPSNPVC
                     EIADIAGLAAIARGAGAILAIDNTMCSPALQRPVALGADLVVHSATKYLDGQGRVLGG
                     AIAGRRDLVEGPLFNFVRTAGPALSPFNAWVSLKGMETLRLRVEAQSARALELARWLE
                     AHPKVEHVHYPGLESHPQHALAMRQQSAGGAVLSFVVRGGREAAWRVIDGTRLVSITA
                     NFGDTKSTICHPATTTHGRIPPAEREAAGIVEGLLRVAAGLEDAGDIRGDLERGLAAA
                     "
     gene            complement(1874..>2570)
                     /locus_tag="VFV55_05545"
     CDS             complement(1874..>2570)
                     /locus_tag="VFV55_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX4943797.1"
                     /translation="RNMGKSLADKILASGITPEIDVVIPIPDSSRPSALEVATKLGKP
                     YREGFIKNRYIGRTFIMPGQELRKRSVRQKLNAMAVEFKDKNVLLVDDSIVRGTTSRE
                     IVQMARECGSKKVFFASAAPPVRFPNVYGIDMPTRSELIATGRDERQVAEAIEADAVL
                     YQDLASLVADVRACNPAIERFDCSCFDGVYITGDVSSDYLAGIEASRLDGESSAGTRL
                     SSYQLDLGLTAVD"
BASE COUNT          401 a          864 c          906 g          399 t
ORIGIN      
        1 gcgccgcggg cacccggcgc tcgatggcag cgcgcgatgg cgcgtcgaag gccgccggat
       61 agtagaggag gtgcccgccc tcgagcgggc agaggcaggt gtcgagatgg tagaagcgct
      121 ggtcggtgag ccgcagtgca atcacctctc cgccgaagcg ccgcgcgatc tcccctttcg
      181 cggcggccgc ggagcggtgg ccgtgagcca gccacagccg cccggctccg cgatcgaaaa
      241 gcgcgtcgcc cgctccctcg aagggcacgt cgcgcggcat gagcgaaacg tcgtagccgc
      301 gatcgaggaa ccagtcggcg aagtacggct cctcgtactg cctttccggg tagcggaagc
      361 gcgacgccag gaatcgccgc cccttcacga ggccggcgtt ggccgtgaag accatgtcgg
      421 gcgaacccgg ttgcgaggca atcaggcgga ccgtggcgtg gtcggtaagg atgtcataga
      481 gggcccgcca ctgctgcttc gccagcgcgt tgtcgatccg gcgcacgttg ccctgcatcc
      541 acgggttgat gatgtacgag acgtcgaagt actcgggcgg gcacatcagg aacacgtgcg
      601 cggtcttcat ggctctccca tttcctgctc gccgcttcgt gggcggcgtt gccagggaca
      661 tgggcggtga cccatgcccc gaatcgctca ggccgcggcg agaccccgct cgaggtcgcc
      721 ccggatgtcc ccggcatcct cgaggccggc cgccacgcgc agcaacccct ccacgatgcc
      781 cgcagcctcg cgctccgcgg gcgggatgcg gccgtgcgtt gtcgtggccg ggtggcagat
      841 cgtcgacttg gtgtcgccga aattcgcggt gatggacacg agccgcgtcc cgtcgatgac
      901 gcgccaggcc gcctcgcgcc cgccgcgcac cacgaacgag agcaccgcac cacccgccga
      961 ttgctggcgc atggcaaggg cgtgctgcgg atgcgactcg agccccgggt agtgcacgtg
     1021 ctcgaccttg gggtgcgcct cgagccagcg ggcgagttcc agcgcgcgcg ccgattgcgc
     1081 ctcgacgcgc aaccttagcg tctccattcc cttgaggctc acccaggcgt tgaagggcga
     1141 gagcgccggc ccggcggtgc gcacgaaatt gaagagcggg ccctcgacga ggtcgcggcg
     1201 ccccgcgatg gcgccgccca gcacgcgccc ctggccgtcg aggtacttcg tggccgaatg
     1261 gacaaccagg tccgcgccga gggcgacggg ccgctgcaat gcggggctgc acatggtgtt
     1321 gtcgatcgcc aggatcgctc ccgcgccgcg cgcgatcgcg gccagcccgg caatgtcggc
     1381 gatctcgcag acgggattcg acggggtctc ggcaaagagg agcttcgtgc gcggccgaag
     1441 cgccgaacgc catgcatcga ggtccgtggc atccacccag gtggtctcga ccccgaagcg
     1501 cccgaggatc tggttgagga gcggcacgac ggtgccgaag acgccgcgcg ccgcgatcat
     1561 gtggtcgccg gcagaaagca gtcccatggc catcgttgcc accgcggcca tgccggtgga
     1621 ggtggccacg caggcctgcg ctccctccag cgccgcgagc cgcgactgga acatcgccac
     1681 ggtcgggttc gtgaagcgcg agtagatgtt gccgggttcc tcgttggcga aacggcgcgc
     1741 cgcctccgcc gcgctcttga agacgaagct cacggtgagc acgagcgctt ccgaatgctc
     1801 gcggaagtcg ctgcgcagtc cccccgcccg gacgcccagc gtgtcgggcc cgtattcgtc
     1861 ggtcatggga ttctcagtcc acggccgtga ggccgagatc gagctggtaa ctcgaaaggc
     1921 gcgtgccggc gctcgactcg ccgtcgaggc gcgaggcctc gatgccggca aggtagtcgc
     1981 tcgacacgtc gccggtgatg tacacgccgt cgaagcagga acagtcgaag cgctcgatgg
     2041 cggggttgca ggcgcgcacg tccgccacca gcgaggcgag gtcctggtaa agcaccgcat
     2101 cggcctcgat ggcttcggca acctggcgct cgtcgcggcc ggtggcgatg agttcggagc
     2161 gcgtgggcat gtcgattccg tagacgttgg ggaagcgcac cgggggcgca gccgaggcga
     2221 agaacacctt cttcgagccg cactcgcgcg ccatctggac gatctcgcgg ctcgtggtgc
     2281 cgcgcacgat cgaatcgtcg acgagaagga cgttcttgtc cttgaactcc accgccatcg
     2341 cgttgagctt ctgccgcacg ctcctcttgc gcagttcctg gcccggcatg atgaaggtgc
     2401 gcccgatgta gcggttcttg atgaagccct cgcgataggg cttgccgagc ttcgtcgcga
     2461 cttcgagcgc gctcggccgg ctggaatcgg ggatcgggat cacgacgtcg atctcgggtg
     2521 tgatccccga ggcgagaatc ttgtcggcaa ggctcttgcc catgttgcgc
//
LOCUS       DASUEV010000171        12109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_967223, whole genome shotgun sequence.
ACCESSION   DASUEV010000171 DASUEV010000000
VERSION     DASUEV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967223"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFV55_05550"
     CDS             <1..569
                     /locus_tag="VFV55_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943798.1"
                     /translation="AVHIGDARVRRFGSPSTVEITRARQEVYQDDRLVPAREASFPSY
                     MPHAPDKAIKGVILSVRGSVSEIAQFSIVTINRGSRDGVEVGHVLATMRRGDQLIRNT
                     RPPLSADFFGSGTKSVATKPNPVVPDAIVASADGTAPATYRPDQAVILPDERSGLLFV
                     FRVFEKLSYGIIMKSVRPISVGDVVQTP"
     gene            697..1782
                     /gene="dprA"
                     /locus_tag="VFV55_05555"
     CDS             697..1782
                     /gene="dprA"
                     /locus_tag="VFV55_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX4943799.1"
                     /translation="MRSDAIEWLRLCLVPGIPGAAQRALLKAFGTPGAAAGADRSSLA
                     AVVDETVADAMVAGPPAELLTATLAWLEHPGHSLLALGDNEYPRDLLQIQDPPTILYA
                     IGDTGLLNRPALAIVGSRNASRQGAIDAREFARSLSDAGFGIASGLALGIDAAAHRGG
                     LAGRSSTLAVVGTGLDRVYPARNRDLAHEIAARGVLVSEFPLGTPPVAANFPRRNRII
                     SGLSRGVLVVEAAMKSGSLTTARLALDQGRDAFAIPGSIHSPLSKGCHWLIKQGAKLV
                     ESADDVLAELDGRPQAPTGLEGPDVGRIEQEPLLEALGYSPASLDALALRTGSPAPEL
                     AADLTLLELEGRVERLPGGLYRQLVVP"
     gene            1852..4362
                     /gene="topA"
                     /locus_tag="VFV55_05560"
     CDS             1852..4362
                     /gene="topA"
                     /locus_tag="VFV55_05560"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX4943800.1"
                     /translation="MASNLLIVESPSKAKTLKKYLGKDFEILASYGHVRDLVPKTGAV
                     DTDDDFRMKYEIIERNAKHVDAIAKAVKAADTILLATDPDREGEAIAWHLFEILKAKR
                     ALKDKKLKRVVFYEITESAVKDAVKNPRDISMELVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLIVEREIEIEGFKSREYWSIHFDSEKAKTPFTAKLTHFKGD
                     KLDQFAVGDAARNAEITGFLESHAKGSARVAEIERKKKLRSPAPPFTTSTLQQEAVRK
                     LGFSTDRAMKVAQSLYEGVSLGKAVTGLITYMRTDSVSLSKEAITEIRGYITKNFGPD
                     YLPKSPIAYKSTAKNAQEAHEAIRPTSIARSPESVAPFLSDEQRKLYEMIWKRALSCQ
                     MTPARFDTVAVDFSVGGPGNTFRATGQTMVFPGYYAVYHEDQDDAVEEEEKRLPALDK
                     GDVVPILKLYGEQHFTQPPPRYSEASLVKVLEQYGIGRPSTYASIISTLQNREYVVLE
                     KKRFQPTDVGRVVTKFLTEHFAHWVDYEFTAKLEDELDDISNGKEEWIPVLERFWKDF
                     SAQIGEKESVSRKDATQEVLDEICPKCKAHNLTIRLGRRGRFIGCPGYPECDYTRNLD
                     GSGDSAEGAAKRDIGVDPKTKLPIQLLQGPFGWYFQLGEAEGDKKPKRVSLPKNVAPE
                     AATLAIALKMLALPRDLGPHPETGKKVVAAIGRFGPYVNHDGQFKSIPKDENVFDISL
                     ERAVALLKEPKSAGSRGALRVLGTHPDDGQPVALYAGKYGPYVKHGKVNATIPDKDAI
                     DTISLEEAVTLLAAKSKSKPKSKAKPRKGKTARVAKAA"
     gene            complement(4399..6825)
                     /gene="gyrB"
                     /locus_tag="VFV55_05565"
     CDS             complement(4399..6825)
                     /gene="gyrB"
                     /locus_tag="VFV55_05565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX4943801.1"
                     /translation="MAQQNEQSNGRHDDEYDSSSIKILKGLEAVRKRPGMYIGDTSDG
                     TGLHHMVFEVVDNAIDEALAGHCDDIKVVIHTDNSISVVDNGRGIPTDIKEDDELKRS
                     AAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSVVNALSEWLKLRVWRNGRAHYIEFRH
                     GVPVEPLKITGETDRRGTEVHFLASPGTFNNIEFHYDILARRLRELSFLNNGTKIELI
                     DQRTGKSENFAYSGGVKGFVEYMNRSKNVLHANVFYAIGEKEGMTVEVSMQWNDSYGE
                     SVQVFTNNIPQRDGGTHLTGLRTAMTRTINNYIEKEEIAKKARVETTGDDMREGLTCV
                     LSVKVPEPKFSSQTKDKLVSSEVQPVVQEIVAQKLAEFLLERPADAKVICTKVVEAAR
                     AREAARKARELTRRKGVLDGVGLPGKLADCQERDPKLCELYLVEGDSAGGSAKQGRDR
                     KFQAILPLKGKILNVEKARFDKMLSSLEVATLITALGTGIGKDEFSLEKLRYHRIIIM
                     TDADVDGAHIRTLLLTFFYRQMPALVENGHIYIAQPPLYKVKSGKDERYLKDEHALKY
                     HQLRRALNGAQLVPGEGAAPLTPEAFEGIATEYLTTEAVIERLSMGMDAGVLRALLQM
                     PRIELDTKEGATLASKAIYDALAPNAPKAHIERDPTSDGWRLRLEKLVHGNRVETILD
                     HAFAGSGDYTHIVNTARTLAGLIQPGAQIKRGETARAIRSFKEGLDWLLDEAKGGMSI
                     QRYKGLGEMNPEQLWETTMDPTKRILLKVQVEDAISADEIFSTLMGDDVEPRRQFIET
                     NALGVSNLDV"
     gene            complement(6864..7970)
                     /gene="dnaN"
                     /locus_tag="VFV55_05570"
     CDS             complement(6864..7970)
                     /gene="dnaN"
                     /locus_tag="VFV55_05570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX4943802.1"
                     /translation="MVKIKATNQALLVPLQHVTGIVERRHTLPILSNVLIQARGGTVD
                     FVATDLEVQIVAKAVLDGDGADGSITVGARKLYDILRALPEDAEVALDAKESRMVVRA
                     GKSRFNLQTLAAADFPRMVESKDAARTLTLPQKALKHALGLVQFAMAVQDIRYYLNGV
                     LFSVEKNMLRVVATDGHRLSFAAEALGAEHESVEAILPRKTVLELIKLLGDSDDPVTL
                     SIGSNQVRFSFGGIEIVSKIVEGKFPDYLKVIPTTHRNKVVLDRVTLAQSLNRAAILS
                     NEKIRGVRLVFTKNALSIICTNNEQEEAEEGLTIDYDGDPIDIGFNISYLLDVLNHVD
                     SETVTVTMGDSNSSALVQMPGNDDFKYVVMPMRI"
     gene            complement(8170..9495)
                     /gene="dnaA"
                     /locus_tag="VFV55_05575"
     CDS             complement(8170..9495)
                     /gene="dnaA"
                     /locus_tag="VFV55_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX4943803.1"
                     /translation="MDSLWLSLVSELKKDLPAQQYDTWIKPLRAERQGNQLRVLAPNH
                     HVLRWIRANLLAKIESRAERVAGLALHVDLVLDERLSPEPEIVLPEPVPIVAERPSRE
                     EHRLNPSFTFANFVNGKANQIARAAAIQVAENPGTAYNPLFIYGGTGLGKTHLLQAIG
                     TDVLKRNPKAKVRYIHAEQFVADVVRAYQHKSFDSLKRYYRSLDLFLIDDVQFFVGKS
                     RSQEEFFYTFNALFEAHKQVAITSDCFPKEMTGIEDRLISRFGWGLTVAVEPPELEMR
                     VAILLKKAEMDGVHVDEAVAFFIAKHIRSNVRELEGALKRVLAYARFNNTTVNVTTAR
                     EALKDLLAVLTRQVSIENIQKTVADYFKIKVSDMYSKKRFRSVARPRQVAMALAKELT
                     QQSLPELGEAFGGRDHTTVLHACRKIQELRDTDQEFSRDYAQLQQILLG"
     gene            9684..9818
                     /gene="rpmH"
                     /locus_tag="VFV55_05580"
     CDS             9684..9818
                     /gene="rpmH"
                     /locus_tag="VFV55_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX4943804.1"
                     /translation="MKRTYQPSNTRRNKTHGFRVRMKTAAGRAVINARRAKGRKQLAV
                     "
     gene            9826..10188
                     /gene="rnpA"
                     /locus_tag="VFV55_05585"
     CDS             9826..10188
                     /gene="rnpA"
                     /locus_tag="VFV55_05585"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX4943805.1"
                     /translation="MARGARKEGFSRRNRFVGRGSFTAALRSPRKFRGPGTVLHVATG
                     RPGESRLGLVTPKRLTRRSVDRNRMKRLAREAFRRHEVKTAGLDLVLAPRQPFSRAGE
                     NAWLTEVQDLFCRAGGNR"
     gene            10190..10399
                     /gene="yidD"
                     /locus_tag="VFV55_05590"
     CDS             10190..10399
                     /gene="yidD"
                     /locus_tag="VFV55_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX4943806.1"
                     /translation="MRRMLVAIIRAYQYVLAPWWGRQCRFTPTCSHYAIEALERHGAL
                     AGSWLALKRILRCHPWHSGGYDPVP"
     gene            10443..>12109
                     /gene="yidC"
                     /locus_tag="VFV55_05595"
     CDS             10443..>12109
                     /gene="yidC"
                     /locus_tag="VFV55_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX4943807.1"
                     /translation="MDIQRLIAFVVFSFSALLLWDAWQKHNAPKVPTPPVAVAGKSGT
                     GTGAIPAMPAAPQAESPGTSAAPGAPAAAPVEANGQPVVVKTDLFEVELNTVGGDVRR
                     VTLYKVFSALDRTKPLTLLAPDPKQYFVTQTGLLGDGLPSHKAVYTVDSNAFSLADGK
                     DQVVVRLVAKDVSGAEVVKKFEFHRGSYEIVVSYEVKNAGDKPIAPHAYFQFLRDGNQ
                     PSQEAAQTSAIAGVATFTGPAVYTDESKFVKVAFKDIDKGKQTHAKQAKDGWIALVQH
                     YFVSAWLPNGTGEREFFTNKVGDNLYSAGVIVPMGAVTPGSSATIAMPLYIGPQETDH
                     LKKIAPGLELVVDYGWLYIIAAPLFWVLKFIHGLVGNWGWAIILLTILIKLVFYPLNH
                     KAGRSMAAMKVLGPKMEKLKQLYGEDRQKLNQAMMELYRTEKINPLGGCLPIVVQIPV
                     FIALYWVLLASIELRHAPWLGWIHDLSGPDPYYILPVIYAASMFVQTKLNPQPADPVQ
                     AKVMLAMPIVFSIFFLFFPSGLVLYWVVQNLLSIAQQWHINRVIAAEA"
BASE COUNT         2087 a         3946 c         3867 g         2209 t
ORIGIN      
        1 aggccgtcca cataggcgac gcccgcgtga ggcgcttcgg ctcgccaagc acggtggaga
       61 tcacgcgcgc ccggcaggag gtttaccagg acgaccggct ggtccccgcc cgagaggctt
      121 cattcccttc gtacatgccg catgcacccg acaaggccat caagggcgtc attctttcgg
      181 tgcgcggaag cgtttcggag atcgcccaat tctcgatcgt caccatcaac cgcggctcgc
      241 gcgatggcgt cgaggtcggg cacgtgctcg ccacgatgcg caggggtgac cagctcatcc
      301 ggaacacgcg gccccccttg tccgccgatt tcttcgggtc cgggacgaaa agcgtggcta
      361 caaagcccaa cccggtcgtg cccgacgcga tcgtggcctc ggcagacggg acggctcccg
      421 cgacctaccg gccggatcag gcggtcatcc ttcccgacga gcgcagcggg ttgctgttcg
      481 tgttccgggt attcgagaag ctttcgtacg gcatcatcat gaagagcgtg cggcccatct
      541 ccgtcggaga cgtggtgcag acgccctgac cggatcgggt gcacgcgcag gaccgggtgg
      601 gggcttctgg cccccacctt cgtttcgggc ggcgtcagcg tgaacggccc tcggcccgtt
      661 gtaaattctg cccctggcca cccgcgaccg actcccttgc gttcagacgc catcgaatgg
      721 cttcgcctct gcctcgtccc cggcatcccc ggggcggcgc agcgcgccct cctcaaggct
      781 ttcggtaccc cgggcgcagc cgccggggcg gaccgatcgt ccctcgcggc tgtcgtcgat
      841 gagacggtcg ccgacgctat ggtcgccggg ccgccggccg aactcctcac ggcgacgctt
      901 gcctggctcg agcatccggg ccatagcctc ctcgccctgg gcgacaacga gtatccccgg
      961 gatctcctcc agatacagga tccgcccacg attctttacg cgatcggcga cactgggctg
     1021 ctcaaccgcc cggcgctggc gatcgtcggc agccggaacg cttcccggca gggcgcgatc
     1081 gacgcacggg aattcgctcg ctccctgtcg gacgccggat tcggcattgc aagcgggctt
     1141 gccctcggca tcgacgcggc cgcccaccgc ggcgggctcg ccggccggtc ttccacgctt
     1201 gcggtcgtgg ggaccggact cgatcgcgtg tacccggcgc gcaaccgcga cctcgcccac
     1261 gagatcgcgg cccgcggcgt actggtatcg gaattcccgc tgggtacgcc tcccgtcgcg
     1321 gccaatttcc cccggcgcaa ccgcatcatc agcggcctgt cgcgcggcgt gctcgtcgtc
     1381 gaggcggcga tgaagagcgg ctccctcacc acggcgcggc tcgcgctcga ccagggccgc
     1441 gacgcgttcg cgattcccgg atcgatccac tccccgctgt ccaagggctg ccactggctg
     1501 atcaagcagg gtgcgaagct cgtcgaatcc gccgacgacg tgctggcgga gttggacggg
     1561 cgcccgcaag cgcccaccgg cctcgaagga cccgacgtcg gtcgcatcga acaggagccc
     1621 ctcctggagg cgttggggta ttcgccggcc agcctcgatg cgctcgcgct ccgcaccgga
     1681 tcgccggcgc cggaactcgc tgcggacctc accctcctgg agctcgaggg acgcgtggaa
     1741 agactgccgg gcgggctcta tcggcaactt gttgtaccct aacgccgcga ttcgggacat
     1801 ggacagcctt ccgattcctg cgttatagat tgaaaaagcg gataccgccc aatggcctca
     1861 aaccttctca tcgtcgaatc gccctcgaag gcgaagacgc tcaagaaata cctcggcaag
     1921 gacttcgaga tcctcgcctc ctacggccac gtccgggacc tcgtgcccaa gaccggggcc
     1981 gtcgataccg acgacgactt ccgcatgaag tacgagatca tcgagcgaaa cgcgaagcac
     2041 gtcgatgcga tcgcgaaggc ggtgaaggcc gcggatacga tcctcctcgc gaccgacccg
     2101 gaccgtgaag gcgaggccat cgcctggcac ctgttcgaga tcctcaaggc caagcgcgcg
     2161 ctgaaggaca agaagctgaa gcgcgtcgtg ttctacgaga taaccgagag cgccgtgaag
     2221 gacgcggtga agaacccgcg cgacatctcg atggagctgg tgaacgcgca gcaggcgcgc
     2281 cgcgcactcg attacctcgt cggcttcaac ctctcgccgc tgctctggaa gaagatccgc
     2341 cgcgggctgt cggccggtcg cgtgcagagc ccggcgcttc gcctcatcgt cgagcgcgag
     2401 atcgagatcg aggggttcaa gtcgcgcgag tactggtcga tccacttcga ctccgagaag
     2461 gcgaagacgc cgttcaccgc gaagctcact cacttcaagg gcgacaagct cgaccagttc
     2521 gccgtcggcg acgcggcgag gaacgcggag atcacgggtt ttctcgagtc ccatgccaag
     2581 ggcagcgcgc gcgtggccga gatcgagcgg aagaagaagc tgcgctcgcc ggcgccgccc
     2641 ttcaccacgt ccacgctgca gcaggaggcc gtgcgcaagc tcgggttctc caccgaccgc
     2701 gcgatgaagg tggcgcagag cctgtacgag ggcgtgtcgc tcggcaaggc cgtcaccggc
     2761 ctcatcacct acatgcgcac ggattcggtg agcctctcga aggaggcgat cacggagatc
     2821 cggggctaca tcacgaagaa cttcggcccg gactacctcc cgaagtcgcc gatcgcctac
     2881 aagagcaccg ccaagaatgc gcaggaggcc cacgaggcca tccggccgac ttccatcgcg
     2941 cgatcgcccg agtccgtggc gccgttcctg agcgacgagc agcgcaagct ctacgagatg
     3001 atctggaagc gcgcgctttc gtgccagatg acgccggcaa gattcgacac cgtggccgtc
     3061 gacttctccg tcggcgggcc gggaaacacc ttccgcgcca ccggacagac catggtcttt
     3121 cccggttact acgccgtcta ccatgaggat caggacgacg cggtggaaga ggaggaaaag
     3181 cgcctgcccg cgctcgacaa gggcgacgtc gtgccgatcc tgaagctcta cggcgaacag
     3241 cacttcaccc agcccccgcc gcgctattcc gaggcaagcc tggtcaaggt gctcgagcag
     3301 tacggcatcg gccggccgtc gacctacgcc tcgatcatct cgacgctgca gaaccgcgaa
     3361 tacgtcgtgc tcgaaaagaa gcgcttccag cctaccgacg tcggccgcgt cgtcaccaag
     3421 ttccttaccg agcacttcgc ccactgggtg gactacgagt tcaccgcgaa gctcgaggac
     3481 gagctcgacg acatttcgaa cggcaaggag gaatggatcc cggtcctcga gagattctgg
     3541 aaggactttt ccgcccagat cggggagaag gaatcggtct cgcgcaagga cgccacgcag
     3601 gaggtcctcg acgagatctg cccgaagtgc aaggcccaca acctgaccat ccgcctcggg
     3661 cgccgcgggc gcttcatcgg atgccccggc tatcccgagt gcgactacac gcgcaatctc
     3721 gacggaagcg gcgattcagc ggagggcgcc gccaagcgcg atatcggcgt ggacccgaaa
     3781 acgaagctgc ccatccagct gctgcagggc cccttcggct ggtatttcca gcttggggag
     3841 gccgaaggcg acaagaaacc caagcgcgta tcgctgccga agaatgtcgc gcccgaagcg
     3901 gccaccctgg cgatcgcgct caagatgctg gcccttcctc gggacctcgg cccgcacccc
     3961 gagacgggca agaaggtcgt cgccgccatc gggcgattcg ggccctacgt gaaccacgac
     4021 gggcaattca agtcgatccc gaaggacgag aacgtcttcg acatcagcct cgagcgcgcg
     4081 gtggccctgc tgaaggaacc gaagtccgcg ggaagccgcg gggcgcttcg cgtcctgggc
     4141 acgcaccccg acgacggcca gcccgttgcg ctctacgcgg gcaagtacgg cccgtacgtg
     4201 aaacatggca aggtgaacgc gacgatcccg gacaaggacg ccatagatac gatttctctc
     4261 gaggaggccg tcacgctcct ggctgcgaaa tcgaagtcga agccgaaatc caaggcgaag
     4321 cccaggaagg gcaagaccgc gcgagtggca aaggccgctt gacctggcgg ccggcggccc
     4381 ggaacccctg tcgccgcctc aaacgtcgag gttggacacg cccagtgcgt tggtctcgat
     4441 gaactggcgc ctcggctcga cgtcgtctcc catgagcgtg gagaagatct cgtcggcgct
     4501 gatcgcatcc tccacctgca ccttgagcag gatgcgtttc gtgggatcca tcgtcgtctc
     4561 ccagagctgc tcggggttca tctcgcccag ccccttgtag cgctggatgg acatgccgcc
     4621 cttcgcttcg tcgaggagcc agtccagccc ctccttgaac gaccggatcg cgcgcgcggt
     4681 ttctccacgc ttgatttgcg cgcccggctg gatgagcccg gcgagcgtcc gggcggtgtt
     4741 cactatgtgc gtgtagtcgc cgctgcccgc aaaggcgtgg tcgaggatgg tctcgaccct
     4801 gttcccgtgc accagcttct cgaggcgcag ccgccagccg tcggaggtag gatcgcgctc
     4861 gatgtgggcc ttgggcgcat tgggtgccag cgcgtcgtag atggccttcg aagccagggt
     4921 cgcgccctcc ttcgtgtcga gctcgatgcg cggcatctgc aacagcgccc gcagcacgcc
     4981 tgcgtccatg cccatcgaca gccgctcgat gaccgcctcc gtggtcaggt actcggtcgc
     5041 gatgccctcg aaggcctcgg gggtgagggg cgcagcgcct tcgcccggca cgagctgcgc
     5101 gccattgagc gcacggcgca gctggtgata cttgagcgcg tgctcgtcct tcaggtaacg
     5161 ctcgtccttg ccgctcttca ccttgtagag cggcggctgc gcgatgtaga tgtgcccgtt
     5221 ctccaccagg gcgggcatct gccggtagaa gaaggtgagc agcaacgtgc ggatgtgcgc
     5281 gccgtcgacg tcggcgtcgg tcatgatgat gatgcggtgg tagcgaagct tctcgaggct
     5341 gaattcgtcc ttgccgattc ccgtgcccag cgcggtgatg agcgtggcca cttcgagcga
     5401 cgaaagcatc ttgtcgaagc gcgccttttc cacgttgagg atcttgccct tgagcggaag
     5461 gatcgcctgg aacttgcggt cgcggccctg cttggccgac ccgccggcgg agtcgccctc
     5521 caccaggtag agctcgcaaa gcttcgggtc gcgctcctgg caatcggcaa gcttgcccgg
     5581 aaggcccacg ccatcgagca cgcccttgcg gcgcgtgagt tcccgcgcct tgcgcgccgc
     5641 ctcgcgggcc cgcgccgcct cgaccacctt cgtgcagatc accttcgcgt cggcgggcct
     5701 ttcgaggagg aactcggcga gcttctgcgc gacgatttcc tgcaccaccg gctgcacctc
     5761 ggaggagacg agcttgtcct tcgtctgcga ggagaacttg ggctccggga ccttaaccga
     5821 gaggacgcag gtgaggccct cgcgcatgtc gtcgccggtg gtctccaccc gcgccttctt
     5881 ggcgatttcc tccttctcga tgtagttgtt gatcgtgcgc gtcatcgcgg tgcgcagccc
     5941 cgtgaggtgc gtgccgccgt cgcgctgcgg gatgttgttg gtgaagacct ggacggactc
     6001 gccgtacgag tcgttccatt gcatcgacac ctcgacggtc atgccttcct tctcgccgat
     6061 cgcgtagaag acgttcgcgt gcaggacgtt cttcgagcgg ttcatgtact cgacgaagcc
     6121 cttcaccccg cccgagtagg cgaagttctc gctcttgccc gtgcgctggt cgatcagctc
     6181 gatcttcgtt ccgttgttca ggaaggacag ctcgcgcagg cgcctggcca ggatgtcgta
     6241 gtgaaactcg atattgttga acgtcccggg agaggcgagg aaatgcacct ccgttccgcg
     6301 ccggtcggtt tcacccgtga tcttcagggg ttccaccggc acgccgtggc ggaactcgat
     6361 gtagtgcgcc cggccgttgc gccagacgcg aagcttgagc cattccgaga gcgcgttgac
     6421 caccgagacg ccgacgccgt gcaggccgcc cgagaccttg taggaattct ggtcgaactt
     6481 gccgccggcg tgcaactccg tcatcacgat ctcggcggcg gagcgcttga gctcgtcatc
     6541 ctccttgatg tcggtgggaa tgccgcggcc gttgtccacg acggagatcg agttgtccgt
     6601 gtggatgacc accttgatgt cgtcgcaatg gcccgccagc gcctcgtcga tcgcgttgtc
     6661 gaccacctcg aagaccatgt ggtgcaggcc cgtgccatcg gaggtgtcgc cgatgtacat
     6721 gcccgggcgc ttgcggaccg cctcgaggcc tttcaggatc ttgatgctgg acgaatcgta
     6781 ttcgtcgtcg tgacgcccgt tcgactgctc gttttgctgt gccattctgt tcctgtggtt
     6841 gcgcgccgga ccgttgcgag gttctagatg cgcatcggca tcacgacgta cttgaaatcg
     6901 tcgttgccgg gcatctgcac gagggcgctg gagttggaat cgcccatcgt caccgtgacg
     6961 gtctccgagt caacgtggtt cagcacgtcg agcaagtagg agatgttgaa gccgatgtcg
     7021 atcggatcgc cgtcgtaatc gatcgtcagg ccctcctccg cctcctcctg ctcgttgttc
     7081 gtgcagatga tggagagggc gttcttggtg aacacgagcc gcacgccgcg gatcttctcg
     7141 ttggacagga ttgccgcgcg gttgagcgac tgcgcgagcg tgacgcggtc gaggacgacc
     7201 ttgttgcggt gcgtggtcgg gatcaccttc aggtaatcgg ggaacttgcc ctcgacgatc
     7261 ttggagacga tctcgatgcc gccgaaggag aagcgcacct ggttcgaccc gatggaaagc
     7321 gtcaccggat cgtcgctgtc gcccaagagc ttgatgagct ccagcacggt cttgcgcgga
     7381 aggattgcct cgacgctctc gtgttccgcg cccagcgctt ccgcggcgaa cgacaggcga
     7441 tgcccgtcgg tggccaccac gcgcagcatg ttcttctcca ccgagaagag caccccgttc
     7501 aggtagtagc ggatgtcctg gacggccatt gcaaactgga cgagcccgag cgcgtgcttg
     7561 agcgccttct gcggaagcgt cagggtgcgg gcggcatcct tggactccac catgcgcgga
     7621 aaatcggcag ccgcgagcgt ctgcaggttg aagcggctct ttccggcacg cacgaccatg
     7681 cggctttcct tcgcgtcgag agccacctcg gcgtcctccg gcaatgcacg caggatgtcg
     7741 tagagcttgc gggcaccgac cgtgatcgaa ccgtcggcgc cgtcgccatc cagcacggcc
     7801 ttcgcgacga tctgcacctc gagatcggtt gccacgaagt cgaccgttcc gccgcgtgcc
     7861 tggatcagca cgttcgaaag tatgggcagc gtgtgcctgc gctccacgat gccggtcacg
     7921 tgctgcaacg gaacaagcag ggcctggttg gtcgccttga tcttgaccat aagggttatc
     7981 tctaactagt taacagttaa taggagacgc cgtgatttcg gtataacctt tttattcatt
     8041 attattcaat tacttgcatt catgctgggc tggggacggt tggcattgga ttgtccataa
     8101 gcctgtgcat aactttgcgt gtccggcggg cgcgcaattc atccacagct ttccccatgc
     8161 tttccagcct catccgagaa ggatctgctg cagttgcgca tagtcacgcg agaattcctg
     8221 gtccgtatcg cgcaattcct ggatcttgcg gcaggcatgc agcacggtcg tatggtcgcg
     8281 tccgccgaac gcctcgccga gttccggcag gctttgctgg gtgagttcct tggcgagcgc
     8341 cattgcgacc tgcctcggcc ttgccacgga ccggaagcgc ttcttcgaat acatgtcgga
     8401 caccttgatc ttgaagtagt ccgccaccgt cttctggatg ttctcgatcg acacctggcg
     8461 cgtcagcacc gcgaggaggt ccttcagcgc ttcccgggcg gtcgtgacat tgaccgtggt
     8521 gttgttgaat cgcgcatagg cgagcacccg cttcagggcg ccctccagtt cgcgcacatt
     8581 ggagcggatg tgcttggcga tgaagaaggc gactgcctcg tcgacatgca cgccgtccat
     8641 ctcggccttc ttgagcagga tcgccacgcg catctcgagc tccgggggct cgacggccac
     8701 ggtgaggccc cagccgaagc gggagatgag gcgatcctcg atcccggtca tctccttggg
     8761 gaagcaatcg ctggtgatcg ccacctgctt gtgcgcctcg aaaagcgcgt tgaaggtgta
     8821 gaagaattcc tcctgcgagc gtgactttcc gacgaagaac tgcacgtcgt cgatgaggaa
     8881 caggtcgagg gagcggtagt agcgcttgag cgagtcgaag ctcttgtgct ggtaggcgcg
     8941 cacgacgtcg gcgacgaact gctcggcgtg gatgtagcgg accttggctt tcgggtttcg
     9001 cttgaggacg tcggtgccga ttgcctgcag caggtgcgtc ttgcccaggc ccgtgccgcc
     9061 atagatgaag agcgggttgt aggcggtgcc ggggttctcg gccacctgga tggcggcggc
     9121 ccgggcaatc tggttcgcct tgccgttcac gaaattggcg aacgtgaagg acggattgag
     9181 gcggtgctcc tcgcgggaag gccgctcggc gacgatggga accggctcgg gaaggacgat
     9241 ttcgggttcc ggcgacagcc tttcgtccag cacgaggtcg acatgcagag ccagccccgc
     9301 cacccgctcg gcccgcgatt cgatcttcgc gagcaggttg gcgcggatcc agcgcaggac
     9361 gtggtggttc ggcgcgagca cgcgcaactg gtttccctgg cgctcggcac gcaggggctt
     9421 gatccaggtg tcgtactgct gcgctggcag gtccttcttg agttcggaaa cgagggaaag
     9481 ccagagcgaa tccatagtgg cggcgggcaa cgcagtcggg ggagaagcag gcagcgggag
     9541 aaacgcgatt ctatagggaa cggggtgagt tatccacagg cccttttcag gtgcaccgca
     9601 gtggcgagat ggcgctcggg tggtcaatcg gctataatcg cgcgcttttc gcctgaaagg
     9661 gcgaaccgat cgatgaggct tccatgaaac gcacttacca gccttccaac acgcgccgca
     9721 acaagacgca tggcttccgt gtccgcatga agacggcagc cggccgcgcg gtgatcaacg
     9781 cccgccgtgc caagggccgc aagcaactcg cggtctgacg ctcgcatggc ccgcggcgcc
     9841 cggaaggaag gcttttcccg gcgcaaccgc tttgtgggtc gcggatcctt cacggccgcc
     9901 cttcgcagcc cacgcaagtt ccgcggtccg ggaaccgtcc tgcatgtggc aaccgggcga
     9961 cccggcgaat cccgcctcgg tctcgtcacg cccaagcgcc tgacgcgtcg ttccgtggac
    10021 cgcaatcgaa tgaagcgact tgcgcgcgag gcctttcgcc ggcatgaggt gaagacggcc
    10081 gggctcgacc tggtgctcgc tccgcgccag ccgttctcgc gtgccggtga gaatgcgtgg
    10141 ctgaccgagg ttcaggacct cttctgccgc gccggcggga atcgatgaaa tgcgccgcat
    10201 gcttgtcgcc atcattcgtg cctaccaata cgtcctggcg ccgtggtggg gccggcaatg
    10261 ccgcttcacg cccacctgct cgcattacgc catcgaggcc ctcgagcgcc atggcgcgct
    10321 ggccgggtcc tggctggcat tgaagcgcat tctgcgttgc catccgtggc attcgggtgg
    10381 ctatgatccg gtcccgtgaa acccgcacgt gacgccattg ttccgttgac gcccgctccc
    10441 tgatggatat ccagcgccta atcgctttcg tcgtcttcag tttttccgcc cttctgctgt
    10501 gggatgcgtg gcagaagcac aatgcgccca aggtgcccac cccgccagtc gccgtggctg
    10561 gaaagtcggg cacgggcacg ggtgcgatac ccgccatgcc ggcagcgcca caagccgaat
    10621 cgccgggaac atcggcggcg cccggggcgc cggcggccgc gccagtcgag gccaacggcc
    10681 agccggtcgt ggtgaagacc gatctcttcg aggtggagtt gaacacggtc gggggcgacg
    10741 tccggcgcgt cacgctctac aaggtttttt ccgccctcga tcgcacgaag ccgctcacgc
    10801 tgctcgcccc cgaccccaag cagtacttcg tcacgcaaac gggcctgctc ggcgatggcc
    10861 tgccatcgca caaggccgtc tacacggtcg attcgaacgc cttcagcctt gccgacggca
    10921 aggaccaggt cgtggtaagg ctcgtcgcca aggacgtttc cggggcggag gtagtgaaga
    10981 aattcgagtt ccatcgtggc agctacgaga tcgtggtgag ctacgaggtg aagaacgcgg
    11041 gcgacaagcc gatcgccccg catgcgtatt tccagttcct tcgcgacggc aaccagccta
    11101 gccaggaggc agcgcagacc agcgccatcg cgggcgtcgc gacgttcacc ggcccggccg
    11161 tgtacaccga cgagtcgaag ttcgtgaagg tcgccttcaa ggacatcgac aagggcaagc
    11221 agacgcacgc gaagcaggcg aaggacggct ggatcgcgct ggtgcagcac tacttcgtct
    11281 ccgcgtggct gccgaacggg actggcgagc gcgagttctt caccaacaag gtgggtgaca
    11341 acctgtacag cgccggcgtg atcgtgccga tgggtgcggt gaccccgggt tccagcgcca
    11401 cgattgcgat gccgctctac atcggtccgc aggaaaccga tcacctgaag aagatcgccc
    11461 cgggactcga gctcgtggtc gactacggct ggctatacat catcgccgcg ccgctgttct
    11521 gggtgctcaa gttcatccac ggcctggtcg gcaactgggg ctgggcaatc atcctcctca
    11581 ccatcctcat caagctcgtc ttctacccgc tcaaccacaa ggcgggccgt tcgatggccg
    11641 cgatgaaggt gctgggcccg aagatggaga agctcaagca gctttacggc gaagaccggc
    11701 agaagctcaa ccaggcgatg atggagctct accgcaccga gaagatcaac ccgcttggcg
    11761 ggtgtcttcc gatcgtcgtc cagattccgg tgttcatcgc gctctactgg gtgctgctcg
    11821 cctccatcga gcttcgacac gcgccgtggc tcggctggat ccacgacctc tccgggcccg
    11881 acccctatta catcctgccg gtgatctacg cggcgtcgat gttcgtgcag acgaagctca
    11941 atccgcagcc ggccgacccg gtgcaggcga aggtgatgct ggcgatgccc atcgtcttca
    12001 gcatcttctt ccttttcttt ccctccggtc tcgtgctcta ctgggtcgtg cagaacctcc
    12061 tgtcgattgc ccagcagtgg cacatcaacc gcgtgatagc ggccgaggc
//
LOCUS       DASUEV010000172         5977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1081575, whole genome shotgun sequence.
ACCESSION   DASUEV010000172 DASUEV010000000
VERSION     DASUEV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081575"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFV55_05600"
     CDS             complement(<1..452)
                     /locus_tag="VFV55_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4943808.1"
                     /translation="MPMIDLRNVSKWYGSFQVLNDCSTRVEKGEVVVVCGPSGSGKST
                     LIKTVNGLEPFQKGEIVVNGISVGDAKTNLPKLRAHIGMVFQNFELFPHMSITENLTI
                     GQVKVLGRTQEQAAEKGLKLLDRVGLLTQKDKFPGQLSGGQQQRVAIAR"
     gene            complement(462..1145)
                     /locus_tag="VFV55_05605"
     CDS             complement(462..1145)
                     /locus_tag="VFV55_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578820.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4943809.1"
                     /translation="MAELDWSVIWKYREVLRDGMLTSLKLFAIALAGGILFGTLLAMA
                     RLSHWKALAVPAAAFVNLIRAIPFIMAIFWFYFLTPMIVSKFTGHQGEAVGPFTSAIV
                     AFIMVESAYYSEIVRAGIQSIPRGQPSAAYALGMNYWQSMGHVVLPQAFRNMLPVMLT
                     QAVILFQDTSLVYVVALKDFLGAASKAGQLTGRIVELYLFVAVVFFIICFAASHAVKL
                     LQAKIAIAK"
     gene            complement(1145..1912)
                     /locus_tag="VFV55_05610"
     CDS             complement(1145..1912)
                     /locus_tag="VFV55_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX4943810.1"
                     /translation="MDWRVFLEQAPSGGMTYLGWLWSGLFWTLAVSILAWVIASVVGC
                     VVGVARTIPVAWARALGTAYVEVFRNIPLLVQMFLWYFVMPEVIPKAWGDWLKQDMPN
                     LLTLPFAAQFSLWEFTAAVLCLGLYTASRVAEQVRSGIQSLPRGQASAGLAMGFTLAQ
                     VYRHVTLPMAFRIIIPPMTSEFLTIFKNSSTALTIGILELTAQSRQISEYTFKTFEAF
                     TAATLIYIAITMTVIFAMRWLEGRVAVPGYISQGSGH"
     gene            complement(1929..2816)
                     /locus_tag="VFV55_05615"
     CDS             complement(1929..2816)
                     /locus_tag="VFV55_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4943811.1"
                     /translation="MIRKSLAAVAALAALVAVPATAQDLTGTLKKIKDSGTITIGHRE
                     TSIPFSYLDEKQQPVGYSMDICAAVVEEVKKELGLAQLTVKYNPVTPQTRIPLMANGT
                     IDIECGSTTNTLTRQKQVGFAPVTFITGTKLLVKKSSKVKSYKDLRNKTVVVTQGTTN
                     ERIIKALSDKDNLNIKFLNAKDHGESFLTVESGRAVAFSMDDILLYGLIAKAKNPKDF
                     EVVGDYLSYDPYGMMYRKGDEDFGLVIRRAIARLMASGDINKLYNKWFLEKLPSGETM
                     GVPMSPLLKAAITLQALPE"
     gene            complement(2919..3632)
                     /locus_tag="VFV55_05620"
     CDS             complement(2919..3632)
                     /locus_tag="VFV55_05620"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX4943812.1"
                     /translation="MKTSAFRPRRVGILGGMGPAAAIDLQEKILAATPATTDAEHLPI
                     VVWNVPQVPDRVAAVRGDGPSPVAAMVEGARALETAGCEAFAVACNTAHHWAGELAAS
                     VRIPMLHIADAAVDALSRRPVPPMRVGILATRGTLAAAFYGERLAQRGFAWIVPAEEE
                     QARFVDEAIARVKAGDVAGARAPFEAAARALAGRGADLILLACTELPLAAEGADSPVP
                     LLDASRALARAVVDFSLRR"
     gene            3751..>5977
                     /locus_tag="VFV55_05625"
     CDS             3751..>5977
                     /locus_tag="VFV55_05625"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943813.1"
                     /translation="MPSPAADPKQSILEPVLRLASQRLSGAAATQAREFLALYYDQVD
                     AEDLSARAPEDLYGAGMANLAFARRFASGTPKLRVYNPRLEEHGWSSPHTVIEIVNDD
                     MPFLVDSVTMELNRQGHTLHLLVHPLVKTVRERDGELASFAPAGREGARESFIHVEID
                     RESDPARLKALGDGLLAVLADVRAAVEDWTPMRAAMHSIVGELATPPAGLDAADVEET
                     RAFLSWALDEHFTFIGYREYELDRAGGEDELKIVPRSGLGVLREPQLGGVSRSFRELP
                     AAIRALAREPRLLVLTKANTRATVHRPGYLDYVGVKKFDAAGRVTGERRFVGLYTTAA
                     YHADPGDIPLLRRKVARILDRAGFPAASHGYKNLKSMLHAYPRDELFQVDEASLFDIA
                     MGVLRLGDRRRTRMFLRRDLYGRFFSCLIYLPRENFNTDTRVKIQEILKRRLAGTSAE
                     YTVQLTDAVLARIHMLVRTLPKDEPRVDAAALEAEIAQATRRWEDDLRLALTEAHGEE
                     RAIPYLRRYGPAFPAAYRDMLSPRTAVRDIAVIETLHAGNPFAVSLYRPVEADSRTLR
                     LRVYRLGAKLALSASLPVLENMGLEAMDEEGWEIEREGEAPVFLHDFGLRSARELADV
                     EAIKAVTEEALLRVARGEIENDAFNRLTPAVALAPDDVVVLRAYARYLRQAGFTFSQA
                     YIAQTLAAHPAIVAMLVAIFQARFAPAAGADREAVQQRLHDEAKAALNGVANLDEDRI
                     LR"
BASE COUNT          961 a         2004 c         2083 g          929 t
ORIGIN      
        1 cgggcgatgg cgacgcgctg ctgctggccg ccggagagct ggcccgggaa cttgtccttc
       61 tgcgtgagga ggcccacgcg gtcgaggagc ttcaggccct tttccgcggc ctgctcctgc
      121 gtgcgcccga gcaccttcac ctggccgatg gtgaggttct cggtgatcga catgtgcggg
      181 aacagctcga agttctggaa caccatgccg atgtgcgcgc gcagcttcgg caggttcgtc
      241 ttcgcgtcgc ccaccgagat cccgttcacg acgatctcgc ccttctggaa cggctccagc
      301 ccgttgaccg tcttgatgag cgtcgacttg cccgagcccg aaggcccgca gaccaccacg
      361 acctcgccct tctccaccct ggtgctgcaa tcgttgagca cctggaacga gccataccac
      421 ttgctcacgt tgcggaggtc gatcatgggc atgggatggt cctatttcgc gatggcgatc
      481 ttcgcctgga ggagcttgac ggcgtgcgag gcggcgaagc agatgatgaa gaagacgacg
      541 gcgacgaaaa ggtagagctc gacgatgcgc ccggtcagct gcccggcctt ggaggccgcg
      601 ccgaggaagt ccttcagggc caccacgtag accagcgagg tgtcctggaa caggatcacc
      661 gcctgggtga gcatcaccgg cagcatgttg cggaacgcct gcggcaggac cacgtggccc
      721 atcgactgcc agtagttcat gccgagcgca taggcggccg agggctggcc gcgcggaatc
      781 gactggatgc ccgcgcgcac gatctccgag tagtaggccg actccaccat gatgaacgcg
      841 acgatcgcgg aggtgaacgg ccccacggcc tcgccctggt ggccggtgaa cttcgacacg
      901 atcatgggcg tgaggaagta gaaccagaag atcgccatga tgaaggggat ggctcggatc
      961 aggttcacga acgccgcggc cggaaccgcg agcgccttcc agtgcgagag ccttgccatg
     1021 gcgagcagcg tgccgaagag gatgccgccg gccagggcga tcgcgaagag cttgagcgag
     1081 gtgagcatcc cgtcgcggag gacctcgcgg tacttccaga tcacgctcca gtctagttcg
     1141 gccatcagtg cccgctgccc tgggagatgt agccgggcac cgccacgcgg ccctccagcc
     1201 agcgcatggc gaagatcacc gtcatcgtga tcgcgatgta gatgagggtc gccgcggtga
     1261 aggcctcgaa ggtcttgaag gtgtactccg agatctggcg gctctgcgcg gtgagttcca
     1321 ggatgccgat ggtgagcgcg gtcgacgagt tcttgaagat cgtgaggaac tccgaagtca
     1381 tcggcgggat gatgatgcgg aaggccatcg gcagcgtcac gtggcggtag acctgcgcca
     1441 gggtgaagcc catcgcgaga cccgcgctgg cctggccgcg cggcagcgac tggattcccg
     1501 agcgcacctg ctcggcgacg cgcgacgccg tgtagaggcc caggcagagg accgcggcgg
     1561 tgaattccca caggctgaac tgtgccgcga agggcagcgt gaggagattg ggcatgtcct
     1621 gcttgagcca gtccccccac gctttcggga tcacctcggg catgacgaag taccacagga
     1681 acatctgcac gaggagcggg atgttgcgga agacctcgac gtaggcggtg ccgagggcgc
     1741 gcgcccaggc gacggggatc gtgcgggcca cgcccaccac gcatcccacc accgaggcga
     1801 tcacccaggc gaggatcgag accgcaagcg tccagaagag acccgaccag agccagccga
     1861 gataggtcat gcccccggac ggcgcctgct cgaggaagac cctccagtcc atcgccttcg
     1921 cccgcgcgtt actccggcag cgcctgcagg gtgatggccg ccttgaggag cgggctcatc
     1981 ggcacgccca tcgtctcgcc ggagggcagc ttctcgagga accacttgtt gtagagcttg
     2041 ttgatgtcgc cgctcgccat cagccgggcg atggcgcggc ggatgaccag gccgaagtcc
     2101 tcgtcgccct tgcggtacat catcccgtac gggtcgtagg acaggtaatc gccgaccacc
     2161 tcgaagtcct tcgggttctt cgccttcgcg atgaggccgt agaggaggat gtcgtccatc
     2221 gagaaagcca cggcgcggcc ggactccacg gtgaggaacg actccccgtg gtccttggcg
     2281 ttgaggaact tgatgttgag gttgtccttg tcggagagcg ccttgatgat ccgctcgttg
     2341 gtcgtgccct gggtcaccac cacggtcttg ttgcgcaggt ccttgtagga cttcaccttc
     2401 gaggacttct tcaccaggag cttggtgccg gtgatgaagg tgacgggcgc gaagccgacc
     2461 tgcttctggc gcgtgagcgt gttggtggtg gagccgcact cgatgtcgat ggtgccgttg
     2521 gccatgaggg ggatgcgcgt ctgcggcgtc accgggttgt acttgaccgt gagctgggcg
     2581 aggccgagct ccttcttcac ctcctcgacc acggcggcgc agatgtccat cgagtagccg
     2641 acgggctgct gcttctcgtc gaggtaggag aagggaatgg acgtctcccg gtggccgatg
     2701 gtgatcgtgc cggagtcctt gatcttcttc agcgtgccgg tgagatcctg cgcggtcgcg
     2761 gggacggcaa cgagtgcggc gagggccgcg acggcggcga gggacttgcg aatcatcgtg
     2821 tttcctcctg ggtttgatcg gtgcgcggga aatcgggggc ctgcagggag cggctaggat
     2881 acgtcgcggc ctcgcacagt ggcaatcgcc gtgcgggctc agcggcgcag ggagaagtcg
     2941 acgacggccc gtgccagggc gcggctcgcg tcgaggagcg ggacgggact gtccgcgccc
     3001 tccgcggcca ggggcaactc ggtgcacgcg aggaggatga ggtccgcgcc gcgcccggcg
     3061 agtgcgcgtg ccgcggcctc gaacggcgcg cgcgcgcccg cgacgtcgcc tgccttcacg
     3121 cgcgcaatgg cctcgtcgac gaatcgcgcc tgctcctcct cggcgggcac gatccaggcg
     3181 aacccgcgct gcgcgagccg ctcgccgtag aaggcggccg caagcgtgcc gcgcgtggca
     3241 aggatcccga cacgcatcgg cgggaccggt cgcctcgaca gggcatcgac cgccgcatcg
     3301 gcgatgtgga gcatcgggat gcgcaccgag gccgccagtt cccccgccca gtggtgcgcg
     3361 gtgttgcacg ccacggcaaa ggcttcgcaa ccggccgtct cgagggcccg cgcgccctcg
     3421 accatcgcgg cgaccggcga ggggccgtcg ccccgtacgg cggccacgcg atcgggcacc
     3481 tgcggcacgt tccagaccac gatgggcagg tgctccgcgt cggtggtggc cggcgtggcg
     3541 gcgaggatct tctcctgcag gtcgattgcg gcggcgggtc ccatgccgcc caggatgccg
     3601 acccggcggg ggcgaaacgc tgaggttttc atggccacat cgtaaacgcg gcgcatcttg
     3661 gtggaaaatg gcggcttcgg acggcttccg gcccgcttcc acgagaagcg tgcccggggc
     3721 cccgcccgcc acgacaacaa cgaggtcgcc atgccttccc ccgccgcgga tcccaagcaa
     3781 tccattctcg agcctgtcct gcgcctggcc agccagcgcc tgtccggcgc cgccgcgacg
     3841 caggcgcgcg agttcctggc gctctactac gaccaggtcg atgccgagga cctgagcgct
     3901 cgggcgcccg aggacctcta tggcgcgggc atggccaacc tggcgtttgc gcggcgcttc
     3961 gcctccggca cgccgaagct gcgcgtgtac aacccgaggc tcgaggaaca cggctggtcc
     4021 agcccgcaca cggtcatcga gatcgtgaac gacgacatgc ccttcctcgt cgactcggtg
     4081 accatggagc tcaaccggca gggccacacg ctgcacctgc tcgtgcaccc gctcgtcaag
     4141 acggtgcgcg agcgcgacgg agagctcgcg tcgttcgcgc ccgccgggcg ggagggcgcg
     4201 cgcgaatcct tcatccacgt ggagatcgac cgcgaaagcg acccggcacg gctgaaggcg
     4261 ctcggcgacg ggctcctcgc ggtgcttgcc gacgtgcgcg cggctgtcga ggactggacg
     4321 ccgatgcgcg cggcgatgca ctccatcgtc ggtgaactcg ccacgccgcc cgcagggctg
     4381 gacgcggccg acgtcgagga aacgcgcgcg ttcctgtcgt gggcgctcga cgagcacttc
     4441 accttcatcg gctatcgcga gtacgagctc gacagggccg gcggcgagga cgagctgaag
     4501 atcgtgccgc gctccggcct gggggtgctg cgcgagccgc agctgggcgg cgtgtcgcgg
     4561 agctttcgcg agctgcccgc agcaatccgg gcgctcgcgc gcgagccgcg gctcctcgtc
     4621 ctcaccaagg ccaacacgcg cgcgacggtg catcggccgg gctacctcga ttacgtcggg
     4681 gtcaagaaat tcgacgccgc cgggcgcgtc accggcgagc ggcgcttcgt cggcctgtac
     4741 accaccgcgg cctaccatgc cgaccccggc gacatcccgc tgctgcgcag gaaggtggcg
     4801 cggatcctcg accgtgccgg gttccccgcg gcgagccacg ggtacaagaa cctcaagtcg
     4861 atgctgcacg cctacccgcg cgacgagctg ttccaggtcg acgaagcctc gctcttcgac
     4921 atcgcgatgg gggtgctgcg cctgggggac cggcggcgca cgcgcatgtt cctgcggcgc
     4981 gatctctacg gccgcttctt ctcctgcctc atctacctgc cgcgcgagaa tttcaacacc
     5041 gacacgcgcg tgaagatcca ggaaatcctc aagcggcggc ttgccgggac gagcgccgag
     5101 tacaccgtcc agctcaccga cgccgtgctc gcgcgcatcc acatgctggt gcgcacgctt
     5161 ccgaaggacg agccgcgcgt cgacgccgct gcgctcgagg ccgagatcgc gcaggctacg
     5221 cgccgctggg aggacgacct gcggctcgcc ctcaccgagg cgcacggcga ggaacgcgcg
     5281 atcccgtacc tgcgccgcta cggaccggcc ttcccggcgg cctaccgtga catgctgtcc
     5341 ccgcgcacgg ccgtgcgtga catcgcggtc atcgagacgc tccatgccgg caacccgttt
     5401 gccgtgagcc tgtatcgccc ggtcgaggcc gattcgcgca cgctgcggct tcgcgtctat
     5461 cgcctcgggg cgaagctggc gctctcggca agcctgcccg tcctcgagaa catggggctg
     5521 gaagcaatgg acgaggaggg gtgggaaatc gagcgcgagg gcgaggcgcc ggtgttcctg
     5581 cacgacttcg ggctgcgcag cgcgcgcgag ctggccgacg tcgaggccat caaggcggtc
     5641 accgaggagg cgctgctgcg cgtcgcacgc ggcgagatcg aaaacgacgc cttcaaccgc
     5701 ctcacgccgg cggtggcact cgctcccgac gacgtggtgg tgctgcgcgc ctacgcccgg
     5761 tacctgcgcc aggccggctt caccttcagc caggcgtaca tcgcgcagac gctggccgcg
     5821 cacccggcca tcgtcgccat gctcgtggcc attttccagg cgcgcttcgc cccggccgcg
     5881 ggcgccgatc gcgaggcggt gcagcagcgc cttcacgacg aggcgaaggc tgctctcaac
     5941 ggcgtggcca acctggacga ggaccgcatc ctgcgcc
//
LOCUS       DASUEV010000173         5217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_622955, whole genome shotgun sequence.
ACCESSION   DASUEV010000173 DASUEV010000000
VERSION     DASUEV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622955"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFV55_05630"
     CDS             complement(<1..203)
                     /locus_tag="VFV55_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4943814.1"
                     /translation="MDRLEALDAWLRDALGGRAFTRQPASADASFRRYFRVSSEDRTW
                     IAMDAPPEREDCRPFVKVASLMRA"
     gene            274..2478
                     /locus_tag="VFV55_05635"
     CDS             274..2478
                     /locus_tag="VFV55_05635"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX4943815.1"
                     /translation="MAPRTLTLVFALACAVSARANEGVPLRLDRRLAPPPPRVESDAV
                     RFLSADHISGSEAKSVTATGNVSLRQRGAAINADRLEYSLETDTAVATGNVRLERLGD
                     SATGPRLVYRIEQGTGEMESPVFDFPKREDRRSASRASAERAILEGEDRNRFLRAEYT
                     TCPVPRDDWTLKVSELVIDGKRQVGTAWNSTVWFLGVPILYSPWLSFPTNNARKTGFL
                     PPTFGSSGRGGFEFSLPWYWNIAESQDATVMPKLFSKRGLQVGGEYRYLRPTYAGQAD
                     VEFLPNDAIADRDRYFLAVRHAQQLPRGWSLAVNAQRVSDDNYFRDLSTRVAATSQTI
                     LPRDAILGYTDETWAVSARAVAYQTLQDPTAPVPATVPYRQVPQLMVSGLKQNVHGFD
                     WQVASELTNYRHPELVDGQRLIAYPQVSWPLRRAWGYVTPKVGFHYTRYHLSDNDGGP
                     GDATRNVPLASVDTGIYLDRPWDFLGGRFQQTLEPRLFYLRVPFRDQSRLPNFSTAEV
                     DFGANSLFRENRFIGGDRIGDANQVTAAITSRLVESDTGLERLNVTLGQIYYFEPQRV
                     TLLGPPREENKSDLVALVSGQVTPAFSIDGGLEYNVSNNRARRLDVAARYSPRPGSLV
                     NAAYRYTRDQVKQVDLSAQWPVTAALSVVGRWNWSLEDRKLIEGLAGFEYNAGCWEIR
                     AVAHRFITATQQVSTSFQIQLELSGLSRIGINPLETLRQNIPGYRRSDEMSR"
     gene            2478..3824
                     /locus_tag="VFV55_05640"
     CDS             2478..3824
                     /locus_tag="VFV55_05640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4943816.1"
                     /translation="MTIRPFPRSAALLMALAAAAAFAQQPAPSRPDARAASPAKSVVA
                     VDRIVAVVNDEVITQNELAERTRLVAGQLQRGGGQLPPPDALQRQILERMINDLVQVQ
                     MAKETNVKVDDATVEKTIQRIADDNNMSMTAFRQALERDGIPYAGFRDDMRNELLLAR
                     LREREVENAIVVTDAEVETELARSGKERGGDTEYRLQHILVMVPQQATPEQIEGRRVR
                     ALQALSDLRKGATFAEVSATFSDAPDALQGGNLGWRAAGRLPTLFLDALEKIQPGEVS
                     DILRSPNGFHIVKLLEKRGKDKAPGITQTHARHILIRTKEGTSDADVRERLVRLRERI
                     VAGEDFAELARVHSEDGTAAKGGDLGWISPGETVPEFERTMNGLRDGEFSQPVQSPFG
                     WHIVQVLGRRTEEMSEERKKLVARQTVRARKGDEAYQDWLRQTRDRAFVENRLDER"
     gene            3853..4827
                     /gene="pdxA"
                     /locus_tag="VFV55_05645"
     CDS             3853..4827
                     /gene="pdxA"
                     /locus_tag="VFV55_05645"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX4943817.1"
                     /translation="MESMPRIAITAGEPAGIGPDLCAILAAKQFAARIVFVGDRGVIA
                     DRASRRGLAFHVPDYFAGATAPVSLLHVAAPAPVESGRLDPANGRHVLALLDRALDGC
                     RTGEFAAMVTAPVQKSTINDAGVPFTGHTEYLAERTGTARVVMMLAGGGLRVALATTH
                     LPLSAVPAAITREGLAATIRIVDRELRERFGIDRPRILVAGLNPHAGESGHMGREEID
                     VILPVLESLRREDIRAEGPLPADTLFTARVLAGADAVVAMYHDQGLPVLKYASFGHAV
                     NVTLGLPVIRTSVDHGTALDLAGTGDVDAGSLEAAVALAIDLARRARR"
     gene            4836..>5217
                     /locus_tag="VFV55_05650"
     CDS             4836..>5217
                     /locus_tag="VFV55_05650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEX4943818.1"
                     /translation="MLPRAKKRLGQHFLTDRHYLDRIVAEIAARPGEVIVEIGPGPGA
                     LTERLAQVVRPLHVVEIDRDLAASLRARFAPDSVVVHEGDALAFDFASLPAGLRVVGN
                     LPYNISTPILFHVASYAPRIRDCVF"
BASE COUNT          824 a         1930 c         1695 g          768 t
ORIGIN      
        1 gcccgcatga gggatgcgac cttcacgaac ggccggcaat cctcgcgttc gggtggcgcg
       61 tccatcgcga tccaggtccg gtcctcggag gagacgcgga aataacggcg gaagctcgcg
      121 tcggcgctcg ccggctggcg cgtgaacgcg cgtccgccga gggcgtcgcg aagccaggcg
      181 tcgagcgcct cgaggcgatc catgggaagt gaagcgaggg ggctttcgcg tgtattcttg
      241 ccgattctag catcgcaccg ccctcttccc tcgatggccc cccgtacctt gacgctcgtc
      301 ttcgccctcg cgtgcgccgt gtcggcacgg gccaatgaag gcgtgcccct caggctcgac
      361 cgaaggctgg ccccgccacc tccccgcgtg gaaagcgatg ccgtgcgctt cctatccgcc
      421 gaccacatct ccggcagcga ggcgaaaagc gttaccgcta ccggcaacgt ctcgctgcgc
      481 cagcgaggcg cggcgatcaa cgccgaccgc ctggaatact ccctcgaaac cgacaccgcg
      541 gtggccaccg gaaacgtgcg cctcgagcgc ctgggcgaca gcgcaaccgg cccgcgcctc
      601 gtctaccgga tcgaacaggg aacgggcgag atggaaagcc ccgtcttcga tttcccgaag
      661 cgcgaggatc gccgcagcgc ttcgcgcgcc agtgccgagc gcgccatcct cgaaggagag
      721 gacaggaacc ggttcctgcg cgccgaatac acgacgtgcc ccgtgccccg cgacgactgg
      781 acgctcaagg tgagcgagct ggtgatcgac ggcaagcgcc aggtcggcac cgcctggaat
      841 tccaccgtct ggttcctcgg cgtgcccatc ctctactcgc cctggctctc cttcccgacc
      901 aacaatgcgc gcaagaccgg cttcctgccg cccaccttcg ggtcctcggg tcgcggcgga
      961 ttcgagttct cgctgccgtg gtactggaac atcgcggaat cccaggacgc gaccgtgatg
     1021 cccaagctgt tctccaagcg cggcctgcag gtgggcggcg agtaccgcta cctgcggccc
     1081 acctatgcag gccaggcgga cgtcgagttc cttcccaacg acgcgatcgc cgatcgcgac
     1141 cgctacttcc tcgccgtgcg gcacgcgcaa cagcttccgc gcggctggtc gctggcggtg
     1201 aacgcgcagc gcgtctccga cgacaactat ttccgcgatc tctcgacgcg cgtggcggcg
     1261 acctcccaga cgatcctccc gagggacgcg atcctgggct acaccgatga aacctgggcc
     1321 gtgtccgccc gcgccgtggc ctaccagacc ctgcaggacc ccaccgcgcc cgtgcccgcc
     1381 acggtcccgt accgccaggt gccgcaactc atggtctcgg gcctgaagca gaacgtccac
     1441 ggattcgact ggcaggtggc gagcgaactc accaactacc ggcacccgga actggtggac
     1501 gggcagcgcc tcatcgccta tccgcaggtc tcctggcccc tgcgccgggc ctggggctac
     1561 gtgacgccga aggtgggctt ccactacacg cgctatcacc tctcggacaa cgacggcggg
     1621 cccggggacg ccacgcgcaa cgtgcccctc gccagcgtgg acaccggcat ctacctcgac
     1681 cggccctggg acttcctcgg cggccgcttc cagcagaccc tcgagccgcg gcttttctac
     1741 ctgcgcgtgc ccttccgcga ccagtcccgg ctgcccaatt tctcgacggc ggaagtcgac
     1801 ttcggggcca attccctctt tcgcgagaac cgcttcatcg gcggcgatcg catcggcgac
     1861 gccaaccagg tcacggccgc aatcaccagc cggctggtcg agtcggacac gggcctcgag
     1921 cgcctgaacg tcacgctcgg gcagatctac tacttcgagc cgcagcgcgt gacgctcctc
     1981 ggcccgccgc gcgaggagaa caagtcggac ctcgtggccc tcgtttccgg ccaggtgacg
     2041 cccgccttct cgatcgacgg cggcctcgag tacaacgtga gcaacaaccg cgcgcggcgc
     2101 ctcgacgtgg cggcgcgcta cagcccgcgg ccaggcagcc tggtaaacgc cgcctatcgc
     2161 tacacgcgcg accaggtgaa gcaggtcgac ctctccgcgc agtggccggt gaccgcggcc
     2221 ctctcggtcg tgggccgctg gaactggagc ctggaggatc gcaagctcat cgaggggctg
     2281 gcgggcttcg aatataatgc gggctgctgg gagatacgcg ccgtcgcgca ccgcttcatc
     2341 acggcaacgc agcaggtatc gacttccttc cagatccagc tcgagctctc gggcctgtcg
     2401 agaatcggca tcaacccgct cgagaccctg aggcagaaca ttcccggcta ccgccggtca
     2461 gacgagatgt cccgatgatg actatccgcc ccttcccgcg atcggccgcc ctgctcatgg
     2521 cgctggccgc cgccgccgcg tttgcccagc agcctgcgcc ctcccggccc gatgcccggg
     2581 ctgcgtcgcc tgcgaaaagc gtcgtggccg tcgaccgcat cgtggcggtc gtcaacgacg
     2641 aggtcatcac gcagaacgag ctcgccgagc gaacccgcct cgtcgcgggc cagctgcagc
     2701 gcgggggcgg ccagttgccg ccgcccgatg ccctccagcg ccagatcctc gagcggatga
     2761 tcaacgacct ggtccaggtc cagatggcca aggagacgaa cgtcaaggtg gacgatgcga
     2821 cggtcgaaaa gacgatccag cgcatcgccg acgacaacaa catgtcgatg accgccttcc
     2881 gccaggcgct ggagcgcgac ggcatcccct acgccgggtt ccgcgacgac atgcgcaacg
     2941 agctgctcct cgcccgcctg cgcgagcgcg aggtcgagaa cgcgatcgtc gtgaccgatg
     3001 cggaagtgga gacggaactc gcccgctccg gcaaggagcg cggcggggac accgagtacc
     3061 ggttgcagca catcctggtg atggtgccgc agcaggccac gcccgagcag atcgagggcc
     3121 ggcgcgttcg cgcgcttcag gcgctgtccg acctgcgcaa gggcgccact tttgccgaag
     3181 tctccgccac attctcggac gcgcccgacg ccctgcaggg cggcaacctc ggctggcgcg
     3241 ccgccggccg gcttccgacg ctcttcctcg atgccctgga aaagatccag cccggggagg
     3301 tgagcgacat cctgaggagc cccaacggct tccacatcgt gaagctcctg gaaaagcgcg
     3361 gcaaggacaa ggccccgggc atcacccaga cccacgcgcg ccacatcctc atccgcacga
     3421 aggagggcac gagcgacgcc gacgtgcgcg aacgccttgt ccgcctgcgc gagcgcatcg
     3481 tcgcgggcga ggatttcgcg gagctcgcgc gcgtgcactc cgaggacggg accgccgcca
     3541 agggtggcga tctcggctgg atttcacccg gcgagaccgt cccggagttc gagcgcacca
     3601 tgaacggcct gcgcgacggc gagttcagcc agccggtcca gtcgccgttc ggctggcata
     3661 tcgtgcaggt cctcgggcgc cgcacggagg aaatgagcga ggagcgcaag aagctcgtcg
     3721 cacgccagac ggtgcgcgcg cgcaagggcg acgaggccta ccaggactgg ctgcgccaga
     3781 cgcgcgaccg cgccttcgtc gagaaccgcc tcgacgagcg ctgatccggg catcgcggcg
     3841 cccgcggcgc gcatggaatc gatgccccgc atcgcgatca ccgcgggcga gcccgcgggc
     3901 atcggccccg atctttgcgc gatccttgcc gcaaagcagt tcgcggcgag gatcgtcttc
     3961 gtcggggacc gcggcgtcat cgccgatcgt gcgagccggc gcgggctcgc cttccacgta
     4021 cccgattatt tcgccggcgc cacggcgccg gtatcgttgc tgcacgtcgc cgcgccggct
     4081 cccgtggagt ccggtcgcct cgatcccgcc aacggccgcc atgtccttgc gctgctcgac
     4141 cgcgccctcg acggctgccg cacgggagag ttcgcggcaa tggtcaccgc tccggtgcag
     4201 aagtcgacga tcaacgacgc gggcgtcccg ttcaccggcc acaccgaata cctcgccgag
     4261 cgcacgggca ccgcgcgcgt cgtgatgatg ctcgccggcg gcgggctccg cgtggcgctc
     4321 gccactaccc acctgccgct ctccgccgtg cctgccgcca tcacgcgcga ggggctcgcc
     4381 gcgacgatcc gcatcgtgga tcgcgagctg cgcgagcgct tcggcatcga ccgcccgcgc
     4441 atcctcgttg cgggactgaa tccgcatgcc ggagagtccg gccacatggg gcgcgaggag
     4501 atcgatgtca tcctgccggt gctcgagtcc ctgcggcgcg aggacatccg tgccgagggg
     4561 ccgctgcccg ccgacacgct attcacggcc cgcgtcctcg ccggcgcgga cgccgtggtc
     4621 gcgatgtacc acgaccaggg cttgccggtg ctcaagtacg cttccttcgg gcatgcggtc
     4681 aacgtcacgc tcggactgcc ggtaatccgc acctcggtcg accacggcac ggcgctggat
     4741 ctcgccggca cgggcgacgt ggacgccggc agcctcgagg ccgcggtcgc gctcgccatc
     4801 gacctcgccc gtcgcgctcg ccgctaggga ccgcgatgct gccgcgggcg aagaaaaggc
     4861 tcggccagca cttcctcacc gaccgccact acctcgaccg catcgtcgcc gagattgccg
     4921 cgaggcccgg cgaggtgatc gtcgagatcg ggccgggccc cggcgcgctc accgagaggc
     4981 tggcgcaggt cgtccggccg ctgcacgtgg tcgagatcga ccgcgatctc gccgcgagcc
     5041 tgcgcgcgcg gttcgcgccg gacagcgtcg tggtgcacga gggcgacgcg ctcgccttcg
     5101 atttcgcctc cctgcctgcg gggctgcgcg tggtcgggaa ccttccttac aacatttcca
     5161 ccccgatcct cttccacgtg gcgtcttacg ccccgcgcat ccgcgactgc gtcttca
//
LOCUS       DASUEV010000174         9936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1082347, whole genome shotgun sequence.
ACCESSION   DASUEV010000174 DASUEV010000000
VERSION     DASUEV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082347"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..609)
                     /locus_tag="VFV55_05655"
     CDS             complement(277..609)
                     /locus_tag="VFV55_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX4943819.1"
                     /translation="MNGDDAKLRDKGLAIQILGRDFRVACPEGEEKQLQSTVEFVNRR
                     MKELRDTGKVTGNERIAIMAALNIAHEFLAQKPAKAGVDGSDFRRRIISMQETLDSAL
                     AADQDKLF"
     gene            complement(606..806)
                     /locus_tag="VFV55_05660"
     CDS             complement(606..806)
                     /locus_tag="VFV55_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943820.1"
                     /translation="MDSELSALEAKVEQVVAQLKQLRAEGRELRQQLAARADENSRLA
                     EKLAAAKTRIEALLKQIPETEP"
     regulatory      909..1076
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1260..3116
                     /locus_tag="VFV55_05665"
     CDS             1260..3116
                     /locus_tag="VFV55_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4943821.1"
                     /translation="MKTSPLAGLAAAVALGAHAQQVSPYPPSATALAFQPLDSIVVTA
                     TRSPQRTVDALRDVEVITRDDIDRAGSISLAELLQRQALVEFRGTGGAGQPAGIFLRG
                     ANAGHTLVLVDGLRASSATVGTTSIENIPLDLIERIEVVKGPLSSLYGPDAIGGVVQI
                     FTRASLKPRLFAAAGYGTDSDSRLSAGFTAIEGKTTVSFSAGWRSVDAPSATNERAFC
                     HDPDRDPYDNAFANLQVLGRLAPGETLAFSGFTSRGRARFDGCPDAQGRFANDRNVQT
                     LSGASVTSAMAYASWWTSRITVGQGRDELSIEGFDPARFETRQDQASWVHEFRTPAGK
                     LLAGVETLRQKVLSATAFAQTRRDTNSVWAGLDESWRGYSLEASARRDDDDQFGARNT
                     GAVSGGLAWPGVGVFSATIGRGFRAPTFFDLYAPSSDFYVANPDLRPERSRSREVALR
                     SEPVAGWQLRLTGFDNRIEDLITYVFPTVLNVRRARIRGLEASAQGTVWGTTVRASLA
                     AQRPRDEDTGFLLQGRAKRFGRLEASRAFGAWSVSGGVTASGERFDSATESADLRLPG
                     YAIADATVRYAAGKGWTVEVVASNLLDRRYEHVVGYDAPRRGVLLNFRFEAP"
     gene            3165..4241
                     /gene="rfaQ"
                     /locus_tag="VFV55_05670"
     CDS             3165..4241
                     /gene="rfaQ"
                     /locus_tag="VFV55_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HEX4943822.1"
                     /translation="MVPDPIGVDSLRRVLVVKLRHHGDVLLASPVFRVLAARAPEAEI
                     DALVYADTRDMLAGHPAVSRIHTIDREWKRQGLLLQARREAALLSELRERRYQLIVHL
                     TDHWRGAWLAQLLRPRWSVAPARSGRLWKACFSHRYALPKGTPRHTVELNLDALRRVG
                     LYPEEEEKRLVMVPGPEAQARVDALLAEHGLAPRGFLQVHPTSRWLFKAWTDERNAEL
                     LQRLAKDGHRLVLTGAPNAREQAIVKRVLGRASVEVTDLSGQLTLREMGALASRARLF
                     FGVDSAPMHIAAAMGTPVVALFGPSGEHEWGPWMVAHRVVTSGHRCRPCGNDGCGGGK
                     VSECLTQLPVDRVHSAINELLAQQ"
     gene            4289..5401
                     /locus_tag="VFV55_05675"
     CDS             4289..5401
                     /locus_tag="VFV55_05675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4943823.1"
                     /translation="MRSRYDAFGGAERFVQSAVAALSVQGASFTLVTRGWPDGDGSAI
                     VLDPFYVGSLWRDRGFARAVCAELPKRHFDLVQSHERIACCDVYRAGDGVHATWLAER
                     ARIQSPLARLATSLSPHHRYLLAAERELFTSPRLRAVICNSEMVRGEIAQRFATPAEK
                     LVLIRNAVDIARFHPGLREEVGGAVREQVGIPRAATVFAFVGSGFERKGVRAFLSALA
                     RRREAAWAIVVGKDKRTARYQRLAARLGIGARVRFVGGVSDVRPYLAAADAFVLPTLY
                     DPLPNAVLEAMAAGLPVVTSTKCGAAELIADGVTGFVHDALDVDGITRSLDRLDPGTA
                     RTMGEAARLAVAHLTPDSMGREYLALYERLLADKLG"
     gene            5409..7157
                     /gene="msbA"
                     /locus_tag="VFV55_05680"
     CDS             5409..7157
                     /gene="msbA"
                     /locus_tag="VFV55_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX4943824.1"
                     /translation="MTAPQPDGRTLYARLLKHVWPYRAALFAAIAAMIVGGLSDAALV
                     KLTGPLVNELFVNRNRELAIYIPLGVVAVFLVSGLASFTSGYLTQWVNNHVILDLREA
                     MFANVLRLPPAYFDEVPTARLVSRFTHDVTQIASASTSVLATLVRDTVTIAALLAILL
                     WSNWRLTLITFLVIPPLALAVRYFSKRLRDMSRASQKAIGGVAEVLDESITNQRVVRV
                     FGGQAYEGGRFAKAAQLIRRYNMKQATAAAATVPVTQLLVACAVAAIIYFAAEQAFAG
                     KTDVGRFVEFIAATGMLLQPLKRLTGVNEHLQRGLAACESVFGLVDEKPEEDHGTVVL
                     DRCRGAVRFEDVSLTYRGGTQPAIDRLSLDIRPGETVALVGASGSGKSSLVHLLPRFY
                     HPTAGRITLDGHPIEDLTLDSLRRQIALVSQHVVLFNDTVAANIAYGQADRAKESDIV
                     NAAEAAHAMEFIRELPQGLATEIGENGAKLSGGQRQRIAIARAILKNAPILLLDEATS
                     ALDTESERAVQAALEALMQGRTTIVIAHRLSTVENADRIVVLAHGRIVEEGAHARLIA
                     AGGIYAGLHRLQFAGS"
     gene            7161..8273
                     /locus_tag="VFV55_05685"
     CDS             7161..8273
                     /locus_tag="VFV55_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX4943825.1"
                     /translation="MAGERLRILHTESSLGWGGQEIRVLTEARGVARRGHEVTLAAPE
                     ESRIFRAASGYGIETVAIPISRKSIAGLHGMMGLVRNRRFDVVNTHSSTDSWLAAVAV
                     SLLPHAPPIVRTRHISAPVPRNFATRWLYREATRRIVTTGERLRLQVIEETGVEPSHV
                     ISIPTGIDLDRFRPGAASEARAALGLERDGVLIGIVATLRSWKGHRYLFEAFAELADR
                     SARLVVVGDGPQREALEALAKEFAIADRVRFAGDQADVVPWMRALDLFCLPSYANEGV
                     PQALMQAMACGLPVITTPVGSITEIVQDGDTGKLVAPESAADLYWGIDRLLADAALRE
                     RLGANARKAALQRFGEDRMVERMLDVFTAVARASHG"
     gene            8266..9372
                     /locus_tag="VFV55_05690"
     CDS             8266..9372
                     /locus_tag="VFV55_05690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX4943826.1"
                     /translation="MGERFATRSAVVARAILRRLAAPGLASAPASPRRILVAHHLLLG
                     DTIMLSPLLAKLRANHPQAEVVMTVPPATLPLYASRPWGVRALAFSPRDSSTVRPLLD
                     EGPFDLAVVPGDNRHAWLAAAMGARHVVAHAGDLPATKNWFVDDARPYPDSPAPWGEM
                     VADLVDGVEPAPCARGDWPDPPAAPVDHPKSAYAVLHVGASTALKRWPADRWAALASA
                     LSQRGLAVVWSAGRGEEALVEACDPGHRHASTAGRLDLAQLWHLLARAALVVSPDTGV
                     SHLGRAIFVPTITLFGPGSAPLCGPGPFWGDAPWRALGEAVFPCRDQTNLFRRDVAWV
                     RRCSRGLDECSEPRCMQAIAVPAVLAAADEFLGV"
     gene            9451..>9936
                     /locus_tag="VFV55_05695"
     CDS             9451..>9936
                     /locus_tag="VFV55_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943827.1"
                     /translation="MTPNGDCAHCYTLHAFSFGIVMSRFAAGLHRFTRLLLAFAIAAA
                     VPAWSAELPVVKGEAVVGSPKSAEVTWLLTTEDEMPARPVRLAAPFERELRAFDAVDS
                     QAKRARVGIGREMAGNAVEGTGDGIVWSRVGEWRIAKLRVQSPGAVAIRVGLRIGATR
                     QP"
BASE COUNT         1432 a         3578 c         3347 g         1579 t
ORIGIN      
        1 tgatgtgtaa agtaacctga ccccttatcg aaaagtaacc tgaccccttt tcaattgcca
       61 agcccttcga ttccctgcgg tgttcgtccg ggccgcacgc tcgaacccac aggttcaagg
      121 cggtcgctca ccaagtgccg caggtacatc cgcttgggac gcgcacaata gcactcatcg
      181 ggcgagcgtc catgcttaac aatttatcgg ctcgaagatg taagcccgga cgaacaccgc
      241 aggaaaccgg caggccggtc gcgcggctcg tggccgctag aagagcttgt cctgatcggc
      301 ggcgagcgcc gaatccagtg tctcctgcat cgaaatgatt ctacgccgaa agtccgaacc
      361 gtcaacgccg gccttcgccg gtttttgcgc gaggaattcg tgcgcgatgt tgagcgccgc
      421 catgatggcg atgcgctcgt tgccggtgac cttgccggtg tcgcgaagtt ccttcatgcg
      481 gcggttcacg aactcgacgg tggactggag ctgcttctcc tcgccctccg ggcacgccac
      541 gcggaaatcg cggccgagga tctggatggc aagccccttg tcgcgcagct tcgcgtcgtc
      601 gccgttcacg gctcggtctc cggaatctgc ttgagcagcg cctcgatgcg cgtcttggcc
      661 gcggcaagct tttccgcgag ccgcgaattc tcgtccgccc gggccgccag ttgctggcga
      721 agctcgcgcc cctccgcgcg cagttgcttc agttgcgcga ccacttgctc gacctttgct
      781 tcgagggcgg agagctcgga atccattcgg tcacactatt ggaaaagcat ttccgagtca
      841 acgtgtaagc tcttattttg aaagtggttt cgaatgtaac agtattcgtt ttattccgcc
      901 gcggagtatc atcgcgtctg cattcgggtg tccgggaagc cggtgaaacg ccggcgctgc
      961 ccccgcaacg gtaagccagt gtggaccacc ctccagccac tgcggccttc aggccgcggg
     1021 aaggcggcgg tcaagtctgg cgagcccgga gaccggcccg atggcaacgg ccccgataaa
     1081 ggggtcaggt tacatttcgg aaaaggggtc aggttacttt tcggtccgcc gtcttgcgac
     1141 ggaaacaagt cagaaaagta acctgacccc ttttccgaaa tgtaacctga cccctttatc
     1201 gggggatcag aacctgtgtg cctgcgggga cgctggctcg cgcggaccaa ggacattcca
     1261 tgaaaacctc gcccctggcc ggcctcgccg ccgcggtggc attgggcgcc cacgcccagc
     1321 aagtctctcc ctatcccccg tcggcaaccg cacttgcatt ccagccgctc gactcgatcg
     1381 tcgtcaccgc cacgcgcagc ccgcagcgca ccgtcgacgc cttgcgcgac gtggaggtca
     1441 tcacgcgcga cgacatcgac cgtgccggat ccatttccct cgccgagctc ctgcagcgcc
     1501 aggcgctggt cgaattccgc ggaaccggtg gcgcgggcca gcccgcgggc atcttcctgc
     1561 gcggcgccaa cgcgggacac accctggtgc tcgtcgacgg cctgcgcgcg agctcggcca
     1621 cggtgggcac gacatccatc gagaacattc cgctcgacct catcgagcgc atcgaagtgg
     1681 tgaagggccc gctgtcgagc ctgtacggcc ccgacgcgat cggcggcgtg gtgcagatct
     1741 tcacgcgcgc atcattgaag ccgcgcctct tcgccgccgc gggctacggc accgattcgg
     1801 actcgcgcct ctccgccggg ttcaccgcga tcgagggcaa gaccaccgtc tcgttctccg
     1861 cggggtggcg atcggtggac gcgccgagcg caaccaacga acgcgccttc tgccacgacc
     1921 ccgaccgcga cccttacgac aacgcgttcg ccaacctgca ggtcctcggc cggctcgcgc
     1981 ccggcgaaac gctcgccttc tccgggttca cgagccgcgg gcgcgcgcgc ttcgacggtt
     2041 gccccgatgc gcagggacgc ttcgccaacg accgcaacgt gcagacgctc tccggcgcga
     2101 gcgtgacttc cgccatggcc tacgcgtcgt ggtggaccag ccgcatcacg gtgggccagg
     2161 gccgcgacga actctcgatc gagggcttcg atccggcgcg cttcgagacg cggcaggacc
     2221 aggcgtcctg ggtgcacgag ttcaggacgc cggccggcaa gctcctcgcc ggcgtggaga
     2281 cgctgcgcca gaaggtgctc tccgccaccg cgttcgcgca gacgcggcgc gataccaact
     2341 ccgtctgggc ggggctcgac gaatcctggc gcggctattc cctggaggcc tccgcccgcc
     2401 gcgacgacga cgaccagttc ggcgcgcgca acaccggcgc cgtgagcggg ggccttgcat
     2461 ggcccggcgt tggcgtcttc tccgccacca tcggccgagg cttccgcgcg ccgaccttct
     2521 tcgatctcta cgccccctcg tcggacttct acgtcgccaa cccggacctt cgcccggagc
     2581 gcagccgaag ccgcgaggtg gcgctgcgaa gcgagcccgt ggcgggatgg caattgcgcc
     2641 tcaccggctt cgacaatcgc atcgaggatc tcatcaccta tgtcttcccg acggtgctga
     2701 acgtgcgccg ggcgcgcatt cgcgggctcg aggcgtcggc gcagggcacg gtgtggggca
     2761 cgaccgtgcg ggcgtcgctc gcggcgcagc ggccgcgcga cgaggatacc ggcttcctgc
     2821 tgcagggccg ggcgaagcgc ttcggacggc tggaagcctc gcgcgcattc ggtgcctggt
     2881 ccgtctcggg cggcgttacg gcgagcggcg agcgcttcga ctcggccacg gaatcggccg
     2941 atttgcggct gcccggctac gcgatcgccg acgccaccgt gaggtacgcc gccggcaagg
     3001 gctggaccgt cgaagtggtc gcctcgaacc tgctcgacag gcgctacgag cacgtcgtgg
     3061 gatacgacgc cccgcggcgc ggcgtgctcc tgaatttccg cttcgaggca ccctgagacg
     3121 ctgcttcttc cccgttgccg tggccgtttg ctaatctgac ggccatggtc cccgacccga
     3181 tcggcgtcga ctcgctgcgg cgcgtgctcg tggtgaagct ccgccaccac ggcgacgtcc
     3241 tgctcgcctc gccggttttc cgcgtgctgg cggcgcgcgc cccggaagcc gagatcgacg
     3301 cgctggtgta cgccgatacg cgcgacatgc tggcgggcca cccggcggtc tcgcgcatcc
     3361 acaccatcga ccgcgaatgg aaacggcagg ggctgctcct gcaggcacgg cgcgaggcgg
     3421 cgctgctctc ggagcttcgc gaacgccgct accagctcat cgttcacctc acggaccatt
     3481 ggcgcggcgc gtggctcgcg cagttgctgc ggccgcgctg gtcggtcgcg ccggcgcgca
     3541 gcggccggct gtggaaggcg tgcttctcgc accgctacgc actgcccaag ggcacgcccc
     3601 ggcacacggt ggagctgaac ctcgacgccc tgcgtcgcgt ggggctgtac ccggaggagg
     3661 aagagaagcg gctcgtgatg gtgcccgggc ccgaggcgca ggccagggtg gatgcgctgc
     3721 tcgcggagca cggcctggcg cccaggggat tcctgcaggt tcacccgacg tcgcgctggc
     3781 tgttcaaggc ctggaccgac gaacgcaatg ccgagctcct gcaacgcctc gcgaaggacg
     3841 ggcaccgcct cgtgctcacc ggcgcgccca atgcgcggga acaggccatc gtcaagcgcg
     3901 tgctgggccg cgccagcgtg gaggtgacgg acctttcggg gcagctcacg ctgcgcgaga
     3961 tgggcgccct cgcgagccgc gcgcgcctct tcttcggcgt ggactccgcg cccatgcaca
     4021 tcgccgccgc gatgggcacg cccgtcgtgg ccctcttcgg cccgagcggc gagcacgaat
     4081 ggggtccgtg gatggtcgcg caccgcgtcg tcacgagcgg ccaccgctgc cgcccgtgcg
     4141 gcaacgacgg ctgcggcgga ggcaaggtga gcgagtgcct tacgcaactg ccggtcgatc
     4201 gcgtgcactc ggccatcaac gagcttctcg cgcagcagtg agcgcggccg cccgaggacg
     4261 aggcaggagc ggcccccgga tagcgctggt gcgaagccgc tacgacgcct tcggcggggc
     4321 cgagcgcttc gtgcagtcgg ccgtcgccgc cctttccgtg caaggcgcct ctttcacgct
     4381 cgtcacgcgc ggctggcccg acggcgacgg cagcgcgatc gtcctcgacc cgttctacgt
     4441 cggcagcctc tggcgcgacc gcggcttcgc ccgcgcggtt tgcgccgagc tcccgaagcg
     4501 ccatttcgac ctggtgcagt cccacgagcg catcgcctgc tgcgacgtct atcgcgccgg
     4561 cgacggcgtg cacgcgacct ggctcgccga gcgggcgcgc atccagtcgc cgctcgcgag
     4621 gctcgccacc tcgctgtctc cgcaccaccg ctatctcctc gccgcggagc gcgagctctt
     4681 cacctccccg cgcctcaggg ccgtgatctg caattcggag atggtacgcg gcgagatcgc
     4741 gcagcgtttc gcgacgccgg ccgaaaagct cgtcctcatc cgcaatgccg tcgacatcgc
     4801 gcgtttccat cccggattgc gggaggaagt cggcggggcc gtgcgcgagc aggtcggcat
     4861 cccgcgcgcg gcgaccgtgt tcgccttcgt ggggtcgggc ttcgagagga agggcgtgcg
     4921 tgcgttcctt tccgccctcg cgcgccggcg cgaagccgcc tgggcgatcg tggtcggaaa
     4981 ggacaagcgc acggcgcgat accagcggct ggctgcgcgg ctcggcatcg gcgcgcgggt
     5041 gcgcttcgtc ggcggcgtct ccgacgtgcg accgtacctg gcggccgccg acgcattcgt
     5101 cctgccgacg ctttacgacc cgctgcccaa cgccgtgctc gaggcaatgg ctgccggcct
     5161 gcccgtggtg accagcacga agtgcggcgc ggcggagctc attgccgacg gcgtgacggg
     5221 cttcgtgcac gacgcgctcg acgtggacgg gatcacgcga tcactcgacc ggctcgaccc
     5281 cgggaccgcc cgcaccatgg gcgaagccgc gcgccttgcc gtcgcccacc tcaccccgga
     5341 ctccatgggc cgcgagtatc tcgccctcta cgaaaggctg ctcgcggaca aactgggata
     5401 atgcccggat gaccgccccg cagcccgacg gccgcacgct atacgcccgg cttctcaagc
     5461 acgtgtggcc ctaccgggcc gcgctcttcg cggccatcgc ggccatgatc gtgggcgggc
     5521 tctccgacgc ggcgctcgtg aagctcaccg ggcccctggt gaacgagctg ttcgtgaacc
     5581 gcaaccgcga gctcgcgatc tacatcccgc tcggcgtggt ggcggtgttc ctggtgagcg
     5641 ggctggcgag cttcacctcc ggctacctca cgcagtgggt caacaaccac gtgatcctcg
     5701 acctgcgcga ggcgatgttc gccaacgtgc tgcgcctccc gcccgcgtac ttcgacgagg
     5761 tgcccacggc gcgcctggtg tcgcgcttca cgcacgacgt gacgcagatc gcctcggcct
     5821 ccacgagcgt gctggccaca ctggtgcgcg acacggtcac catcgccgcg ctgctcgcga
     5881 tcctgctctg gtccaactgg cgcctcacgc tcatcacctt cctcgtgatc ccgcccttgg
     5941 cgctcgcggt gcgctacttc agcaagcgcc tgcgcgacat gagccgcgcc agccagaagg
     6001 cgatcggcgg cgtggccgaa gtgctggacg agtcgatcac caaccagcgc gtggtgcgcg
     6061 tcttcggcgg ccaggcctac gagggcgggc gcttcgcgaa ggccgcgcag ctcatccgcc
     6121 gctacaacat gaagcaggcg accgcggcgg ccgcaacggt gccggtgacg caactcctcg
     6181 tcgcctgcgc ggtggccgcg atcatctact tcgccgccga gcaggccttc gccgggaaga
     6241 cggacgtggg caggttcgtc gagttcatcg ccgccaccgg catgctgctg cagccgctga
     6301 agcgcctcac cggcgtgaac gagcatttgc agcgcggtct agccgcgtgc gagagcgtgt
     6361 tcgggctcgt cgacgagaag cccgaggagg accacggcac cgtggtgctc gatcgctgca
     6421 ggggtgccgt gcgcttcgag gacgtgtccc tcacctaccg cggcggcacg cagccggcga
     6481 tcgaccgcct ttcgctcgac atccgccccg gcgagacggt cgcgctcgtg ggcgcctccg
     6541 gcagcggcaa gtcgagcctc gtccacctgc tgccgcgctt ctatcacccc actgccgggc
     6601 gcatcaccct cgacggccac ccgatcgagg atctcaccct cgacagcctg cggcggcaga
     6661 tcgcgctcgt ctcgcagcac gtggtgctgt tcaacgacac ggtcgcggcc aacatcgcct
     6721 acgggcaggc ggaccgggcg aaggaatccg atatcgtgaa cgcggccgag gccgcccacg
     6781 ccatggaatt catccgcgag ctgccgcagg ggctcgccac ggaaatcggc gagaacggcg
     6841 ccaagctctc cggcggccag cgccagcgca tcgccatcgc gcgcgcgatc ctcaagaacg
     6901 ccccgatcct gctcctggac gaggccacct cggcgctcga caccgagagc gagcgcgcgg
     6961 tgcaggcggc cctggaggcg ctgatgcagg ggcgcaccac gatcgtgatc gcgcatcgcc
     7021 tgtccacggt cgagaatgcc gaccgcatcg tcgtcctcgc gcacggccgc atcgtcgagg
     7081 agggcgcgca cgcccggctg atcgccgcgg gcggcatcta cgcgggattg caccggctgc
     7141 agttcgccgg gtcctagccc gtggccggcg aaaggcttcg catcctgcac accgagtcct
     7201 cgctgggctg gggcggccag gagatccgcg tgctcaccga ggcgcgcggc gtcgcccgcc
     7261 gaggccacga agtgaccctc gccgcgccgg aagagtcgcg catcttccgg gcggcctccg
     7321 gatacggcat cgagacggtg gcaatcccga tctcgcggaa gtcgatcgcg gggctgcacg
     7381 ggatgatggg gctggtgcgc aaccgtcgct tcgacgtggt aaacacgcac agctccaccg
     7441 acagctggct ggccgccgtg gccgtcagct tgttgcccca cgccccgccg atcgtgcgca
     7501 cgcgacacat ctcggcgccc gtgccgcgaa atttcgccac acgctggctc taccgggagg
     7561 cgacgcggcg catcgtgacg accggcgagc gccttcgcct gcaggtcatc gaggaaacgg
     7621 gcgtggagcc gtcccacgtg atttccattc ccaccggcat cgacctcgat cgcttcaggc
     7681 ccggcgcggc gtccgaggcg cgcgcggccc tgggcctcga gcgtgacggc gtgttgatcg
     7741 gcatcgtcgc cacgctgcgc agctggaagg ggcaccgcta cctcttcgag gctttcgcgg
     7801 agctggccga caggagcgcg cggctggtcg tggtgggcga cgggccacag cgcgaggcgc
     7861 tggaggcgct ggcgaaggaa ttcgccatcg ccgatcgcgt gcgcttcgcg ggggaccagg
     7921 ccgacgtcgt gccgtggatg cgcgccctcg accttttctg cctgccttcc tatgcgaacg
     7981 agggcgtgcc gcaggcgctc atgcaggcga tggcctgcgg cttgccggtg atcaccacgc
     8041 ccgtcggcag catcaccgag atcgtgcagg atggcgatac gggcaagctg gtggcgccgg
     8101 agagcgcggc cgacctgtac tggggcatcg acaggctgct tgccgacgca gccctgcgcg
     8161 agcggctcgg cgcgaacgcg cgcaaggctg cattgcaacg cttcggcgag gatcgcatgg
     8221 tggagcggat gctcgacgtc ttcaccgcgg tcgcgagggc ttcgcatggg tgaacgattc
     8281 gccacgcgca gcgcggtcgt cgcgcgcgcg atcctgcgcc gcctcgccgc accggggctc
     8341 gcaagcgcgc ccgccagtcc gcgccgcatc ctcgtggcgc atcacctcct cctcggcgac
     8401 acgatcatgc tctcgccgct gctcgccaag ctgcgcgcga accatccgca agcggaggtc
     8461 gtgatgaccg tcccgcccgc cacgctgccg ctctacgcct cgcgcccgtg gggcgtgcgc
     8521 gcgctggcgt tctcgccgcg cgactcctcg accgtgcgcc cgcttctcga cgaaggtccg
     8581 ttcgatctcg ccgtcgtgcc gggcgacaac cgccatgcat ggctcgcggc tgcaatgggc
     8641 gcacgccacg tggtcgccca cgcgggcgac ctgccggcca ccaagaactg gttcgtcgac
     8701 gatgcgcggc cctaccccga ctcgccggcg ccctggggcg agatggtggc cgacctggtc
     8761 gacggggtgg agccggcgcc ctgcgcgcgc ggtgactggc cggacccgcc cgcggctccc
     8821 gtcgatcacc cgaagtccgc gtatgcggtc ctgcacgtag gcgccagcac ggcgctcaag
     8881 cgctggcccg ccgatcgctg ggcggcgctg gcctcggcgc tgtcgcaacg cggactggct
     8941 gtcgtctgga gcgcggggcg aggcgaggag gcgctggtcg aggcctgcga ccccggccac
     9001 cgccacgcat cgaccgcggg ccgcctggat ctcgcgcaac tctggcacct gctcgcacgg
     9061 gcagcgctgg tcgtttcccc ggacacgggc gtttcccatc tcggccgcgc catcttcgtc
     9121 cccacgatca cgctgttcgg gcccggctcc gccccgctgt gcggcccggg accattctgg
     9181 ggcgatgcgc cgtggcgggc gctgggcgag gcggtctttc cgtgccgcga ccagacgaac
     9241 ctcttccggc gcgatgtcgc ctgggtgcga cgctgctctc gcgggctcga cgagtgttcc
     9301 gaaccgcgct gcatgcaggc aattgccgtg ccggccgtcc tcgccgccgc cgacgagttc
     9361 ctcggtgtct gacacggtgt ccgacaccat gtcagacacc gcccaggaac cacgcgcgct
     9421 tcgcgcggtc cgaactgggc aggtcgtgac ttgaccccga acggggattg cgcgcactgc
     9481 tacactcttc acgcattttc cttcggaatc gtcatgtcgc gtttcgctgc cgggctgcac
     9541 cgattcaccc gtcttctcct cgccttcgcg atcgccgccg cggtacccgc atggtcggcg
     9601 gagcttccgg tggtcaaggg cgaggccgtc gtcggatcgc ccaagagcgc ggaagttacc
     9661 tggctcctca cgaccgagga cgaaatgccc gcgcgccccg ttcgcctcgc ggcgcctttc
     9721 gagcgcgaat tgcgcgcatt cgacgcggtc gattcgcagg ccaagcgcgc ccgggtcggc
     9781 attggccgcg aaatggccgg gaacgcggtg gaaggcaccg gtgacggcat cgtctggagc
     9841 cgcgtgggcg aatggcgcat cgccaagctg cgcgtccagt cgcccggtgc ggtggcgatt
     9901 cgcgtcggct tgcgcatcgg cgcgacgagg cagccc
//
LOCUS       DASUEV010000175         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1083175, whole genome shotgun sequence.
ACCESSION   DASUEV010000175 DASUEV010000000
VERSION     DASUEV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083175"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1045)
                     /gene="rplA"
                     /locus_tag="VFV55_05700"
     CDS             complement(329..1045)
                     /gene="rplA"
                     /locus_tag="VFV55_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX4943828.1"
                     /translation="MAHVSKRAKQINALVDRSKAYEVKTALDLVKKTATAKFDESIDV
                     AVNLGIDAKKSDQVVRGSVVLPRGTGKTVRVAVFAQGANAEKAKNAGADIVGFDDLAE
                     KIKGGYLDFDVVIATPDAMKVVGPLGQVLGPRGLMPNPKVGTVTPNVTEAVKNAKAGQ
                     VQYRADKAGIVQCTIGRASFPVEALAENFEALLGAINKSKPSTSKGVYLKKVSVSSTM
                     GVGIRVDHSTLAGASAGSAQ"
     gene            complement(1047..1478)
                     /gene="rplK"
                     /locus_tag="VFV55_05705"
     CDS             complement(1047..1478)
                     /gene="rplK"
                     /locus_tag="VFV55_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX4943829.1"
                     /translation="MAKKVVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKMEPGLMLPVVITAYADKSFTFILKSPPASVLIRKALKLEKGSKTPHTDKVGKLT
                     RKQAEEIAKTKQPDLTAADLDAAVRTIAGSARSMGVEVEGV"
     gene            complement(1535..2068)
                     /gene="nusG"
                     /locus_tag="VFV55_05710"
     CDS             complement(1535..2068)
                     /gene="nusG"
                     /locus_tag="VFV55_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX4943830.1"
                     /translation="MGKRWYVVHAYSGFEKSVQRALEDRISRAGMQDKFGKILVPVEE
                     VVEMKGGQKHMSERKFFPGYVLVEMEMDDDTWHLVKNTNKVTGFVGGTAMKPTPISEK
                     EVNAIMQQMREGVEKPKPKVLFENGEMVRVKDGPFTDFQGTVEDVNYEKSRLRVAVTI
                     FGRSTPVELEFGQVEKA"
     gene            complement(2080..2439)
                     /gene="secE"
                     /locus_tag="VFV55_05715"
     CDS             complement(2080..2439)
                     /gene="secE"
                     /locus_tag="VFV55_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX4943831.1"
                     /translation="MTNTDIAKLAIAVVLVAAGIAGYYWLGDSALVLRILAVVAGIAA
                     GAAVAAFTAPGREFIVFAREAVVEVKKVVWPTRKETVQTTAAVFAFVLVMAVFLWLSD
                     KTLEWVLYDLVLGWKKT"
     gene            complement(2449..2524)
                     /locus_tag="VFV55_05720"
     tRNA            complement(2449..2524)
                     /locus_tag="VFV55_05720"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2489..2491),aa:Trp,seq:cca)
     gene            complement(2543..>2679)
                     /gene="tuf"
                     /locus_tag="VFV55_05725"
     CDS             complement(2543..>2679)
                     /gene="tuf"
                     /locus_tag="VFV55_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX4943832.1"
                     /translation="VMPGDNIKMVVTLITPIAMEQGLRFAIREGGKTVGAGVVAKVIE
                     "
BASE COUNT          392 a          930 c          812 g          545 t
ORIGIN      
        1 cggagatctc cgccacgact tccttcttgc tttctagatt cagactcaag gtctttctcc
       61 ttctgctgtt cggtgcgtcg cgctcgcggg cccttaagaa tgcccggtcg gcgccacacc
      121 acccatagcg acctttcgtc aggagaccgg ttgcgccccg ggtggggcgc gagaatcctg
      181 tcttgggttc gctatctgcg taggcttcgg ggtctcatcc cctcgattga gcccttctgg
      241 atcacccggg ggtccgggtt caccgcgagg gcgcctacgg tcttggataa cccgccgccg
      301 gcccttgcgg gccggcggcg gcccaaagct actgcgccga gccggcggaa gcgccggcca
      361 gcgtcgaatg atcgactcga atgccgaccc ccatcgtgct ggagacggaa accttcttca
      421 ggtacacgcc cttggaggtc gacggcttcg acttgttgat cgcaccgagg agcgcctcga
      481 agttctcggc aagcgcctcg accgggaagg acgcgcgccc gatcgtgcat tgcacgatcc
      541 ccgccttgtc ggcgcggtac tggacctggc cggccttggc gttcttgacg gcctccgtca
      601 cgttcggcgt cacggtcccg accttcgggt tgggcatcag gccgcggggg cccagcacct
      661 gcccgagcgg gccgacgacc ttcatggcgt ccggcgtggc gatgaccacg tcgaagtcca
      721 ggtacccgcc cttgatcttc tccgcgaggt cgtcgaagcc gacgatgtcg gcgccggcgt
      781 tcttcgcctt ctcggcgttg gcgccctgcg cgaacaccgc gacccgcacc gtcttgccgg
      841 tgccgcgcgg cagcaccacg ctgccccgga ccacctggtc ggacttcttg gcatcgatgc
      901 cgaggttcac cgcgacgtcg atggactcgt cgaacttggc cgtggcggtc ttcttgacca
      961 ggtccagggc ggtcttcacc tcgtaggcct tgctgcggtc gacgagtgcg ttgatctgct
     1021 tggcgcgctt ggagacgtgt gccatgtcag acgccctcca cttcgacgcc catgctgcgc
     1081 gcgctgccgg cgatggtgcg cacggcggcg tcgaggtcgg cggcggtcag gtccggctgc
     1141 ttggtcttgg cgatctcctc ggcctgcttg cgggtgagct tgcccacctt gtcggtgtgc
     1201 ggcgtcttgc tgcccttctc gagcttgagc gccttgcgga tcagcaccga agcgggcggc
     1261 gacttgagga tgaaggtgaa gctcttgtcc gcgtacgcgg tgatcaccac gggcagcatc
     1321 aggcccggtt ccatcttctg cgtctgggcg ttgaacgcct tgcagaattc catgatgttg
     1381 aggccgcgct ggccgagcgc ggggccgatg ggcggcgagg gattcgcctt gcccgccggc
     1441 acttgcagct tgatgaagcc gacaactttc ttcgccatga tggctctcct gtcgggtgca
     1501 agcggacgcc gcagcgccct ccccgtctaa acgctcaggc cttctcgacc tggccgaatt
     1561 caagctcgac cggagtcgag cgtccaaaaa tcgtgaccgc cacgcgcagg cggctcttct
     1621 cgtagttcac gtcttccacg gtgccctgga aatcggtgaa cgggccgtcc ttcacgcgca
     1681 ccatctcgcc gttctcgaac agcactttcg gcttgggctt ctccacgcct tcgcgcatct
     1741 gctgcatgat ggcgttgacc tccttctcgg agatcggcgt cggcttcatc gccgtgccgc
     1801 cgacgaaccc cgtcaccttg ttggtgttct tcaccaggtg ccaggtgtcg tcgtccattt
     1861 ccatttccac cagcacgtag ccgggaaaga acttgcgctc cgacatgtgc ttctggccgc
     1921 ccttcatttc gacgacctcc tccaccggca ccaggatctt gccgaacttg tcctgcatgc
     1981 cggcgcgcga gatgcggtcc tcgagcgcgc gctgcaccga cttctcgaag ccggagtacg
     2041 cgtgcaccac gtaccagcgc ttgcccatgt ccgcttgctt caggtttttt tccatccgag
     2101 gaccaggtcg tataacaccc actcaagtgt cttgtcgctc aaccacagga acaccgccat
     2161 caccagtacg aaggcgaaca ccgcggcggt cgtctgcacc gtttccttgc gcgtcggcca
     2221 caccaccttc ttcacctcca ccaccgcctc gcgcgcgaag acgatgaact cgcggcccgg
     2281 cgccgtgaac gccgccacgg cggcgcctgc cgcgatacct gccaccaccg ccaggatgcg
     2341 cagcacgagc gcgctgtcgc ccagccagta gtagccggcg attccggccg ccaccaggac
     2401 gacggcgatt gcgagcttgg cgatgtcagt gttggtcatg tgcgtgtctg gcaggggcag
     2461 agggcatcga acccccaacc ttcggttttg gagaccgacg ctctgccaat tgagctatgc
     2521 ccctgtcgtg ttgtccggtc gcttactcga tcaccttggc gacgacgccg gcgccgacgg
     2581 tcttgccgcc ctcgcggatg gcaaagcgca agccctgctc catcgcgatc ggcgtgatca
     2641 gcgtcaccac catcttgatg ttgtcccccg gcatcacca
//
LOCUS       DASUEV010000176         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_969802, whole genome shotgun sequence.
ACCESSION   DASUEV010000176 DASUEV010000000
VERSION     DASUEV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969802"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFV55_05730"
     CDS             complement(<1..202)
                     /locus_tag="VFV55_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4943833.1"
                     /translation="MIAFLAAAAAYLVGSIPFAVIVSRAMGLPDPRSFGSGNPGATNV
                     LRTGSKPAAVLTLGGDALKGWVA"
     gene            268..630
                     /locus_tag="VFV55_05735"
     CDS             268..630
                     /locus_tag="VFV55_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX4943834.1"
                     /translation="MDTIFIREFRVDAWVGIYDWEKLRPQTLELEVEIGIPGTAAGKS
                     DDIHDTIHYGLVVERFTAELAERRFGLLEALAEHLAGIVTGELKAPWVRLSVAKLGHI
                     RNVRKVGVSIERTRAPAK"
     gene            638..832
                     /locus_tag="VFV55_05740"
     CDS             638..832
                     /locus_tag="VFV55_05740"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX4943835.1"
                     /translation="MLKWVLVIAVAVVVLSFFGPQFAKMGLGRLPGDITVRFRGRLVY
                     LPITTTVLISLALTALGRIL"
     gene            complement(829..1638)
                     /locus_tag="VFV55_05745"
     CDS             complement(829..1638)
                     /locus_tag="VFV55_05745"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX4943836.1"
                     /translation="MQSNEPGIPLPGPAANDPGPARLVMPGRKVRAGAGVDWVGDGWR
                     LFRKASLMWIVFLVLFTLIHLGMGMVPWVGMWIGNLVSPILLGGIALGCRSLETGGDL
                     ELEHLLAGFRRNTGLLFAIGVVYALGELALFGVFGLFAGPSFLSAILRGDEVAMASSL
                     PDDPMQLALGALVTAALAIPLLAAYWFAPALAMLHGVPAIPAMKASLVACVRNAVPML
                     VNGLVMGALLVPAILPLGLGLVVWAPLMLATIYVSYRSIFTEADELAEDAP"
     gene            complement(1675..>2156)
                     /locus_tag="VFV55_05750"
     CDS             complement(1675..>2156)
                     /locus_tag="VFV55_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEX4943837.1"
                     /translation="GKHTGTPATIFLAQHGVAYTEHIYDYVEHGGTAVSSSSLGVPEH
                     EVVKTLVMETDKGEPLVVLMHGDRKVATKELARMAGAKRVFPCKPEDATRHSGYLVGG
                     CSPFGTRKRMRVYMERSILELPRIYINGGRRGYLLGMPPAEITRVLSPVLVDVGREE"
BASE COUNT          368 a          736 c          737 g          315 t
ORIGIN      
        1 ccgcgaccca gcccttgagc gcgtcgccac ccagcgtgag cacggcggcc ggcttgcttc
       61 ccgtgcgcag cacgttggtg gcgcccggat tgccggagcc gaaggaccgc gggtccggaa
      121 ggcccatggc acggctcacg atcaccgcga acgggatcga gccgaccagg taggctgcgg
      181 cggcggcaag gaacgcgatc atgtcgatac tctacaatac gacctcgaaa aagctcccct
      241 tccgcccccc ggcggcgcgc ccttccgatg gacacgatct tcatccgcga attccgagtc
      301 gacgcctggg tcggcatcta cgactgggag aagctgcgcc cccagacgct cgagctcgaa
      361 gtcgagatcg gaatcccggg aaccgccgcg ggaaagtcgg acgacatcca cgacaccatc
      421 cactatggcc tggtcgtcga gcgcttcacg gccgaactgg ccgagcggcg tttcgggctg
      481 ctggaggcgc tggccgaaca ccttgcgggc atcgtcaccg gcgaattgaa ggccccctgg
      541 gtgcgtcttt ccgtcgccaa gctgggccac atccgcaacg tgcgcaaggt gggcgtgtcg
      601 atcgagcgca cgcgggcgcc ggcgaaatga gcgcgcgatg ctgaagtggg tgctggtcat
      661 cgcggtggcc gtggtggtcc tctcgttctt cggcccgcag ttcgcgaaga tggggctggg
      721 gcggcttccg ggcgacatca ccgtgcgttt ccgcgggcgc ctcgtgtacc tgcccatcac
      781 caccacggtg ctcatctcgc tcgccctcac ggcgctgggg cggatcctct agggcgcgtc
      841 ctccgccaac tcgtcggcct cggtgaatat cgagcggtag gagacgtaga tcgtcgcgag
      901 catcagcggc gcccacacga cgagccccag gccgagcggg aggatcgccg gcacgagaag
      961 cgcccccatc accaggccgt tgacgagcat cggcacggca ttgcgcacgc aggcgaccag
     1021 gctcgccttc atcgccggga tggccggcac gccgtgcagc atggcgagcg ccggcgcgaa
     1081 ccagtaggcc gccaggagcg ggatggcaag cgcagcggtg acgagcgcgc ccagggcgag
     1141 ctgcatcggg tcgtcgggta gcgagctggc catcgccacc tcgtcgccgc gcaggatcgc
     1201 ggacaggaat gacggccccg cgaacaggcc gaagacaccg aacagcgcca gttcgcccag
     1261 cgcgtacacg acgccgatgg cgaagagaag gcccgtgttg cgccggaaac ccgccagcag
     1321 gtgctcgagc tcgagatcgc ctcccgtttc gagggaccgg caacccaggg cgatgccgcc
     1381 gagcaggatc ggcgaaacga gattcccgat ccacatcccg acccagggca ccatgcccat
     1441 cccgaggtgg atgagggtga agaggacgag gaagacgatc cacatcaggc tcgccttccg
     1501 gaagagccgc cagccgtcgc cgacccagtc cacgcccgcg ccggcgcgca ccttgcggcc
     1561 cggcatgacg agcctggcgg gacccggatc gttcgcggct ggcccgggaa gcggaatccc
     1621 cggctcgttg gattgcatgc gtgcgtccgt cctgcggttg ggggcgagcg aaggttactc
     1681 ttcgcgcccc acgtcaacaa ggacgggcga aaggacgcgc gtgatttccg cggggggcat
     1741 gccgagcagg tagccgcgcc gcccgccgtt gatgtagatg cgcggcaact cgaggatcga
     1801 gcgctccatg tacacccgca tccgctttcg cgtgccgaag ggggagcagc caccgacgag
     1861 gtagccggag tggcgcgtcg cgtcctcggg cttgcagggg aagacgcgct ttgcgcccgc
     1921 cattcgcgcg agctccttcg tcgctacctt ccggtcgccg tgcatcagca ccacgagggg
     1981 ctcgcccttg tcggtctcca tgacgagcgt cttcaccacc tcgtgctcgg gcacgccgag
     2041 cgacgaggac gacaccgccg ttccgccgtg ttcgacgtaa tcgtagatgt gctcggtgta
     2101 agccacgccg tgctgcgcga ggaagatggt agccggggtt cccgtatgct ttccca
//
LOCUS       DASUEV010000177         6793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_498816, whole genome shotgun sequence.
ACCESSION   DASUEV010000177 DASUEV010000000
VERSION     DASUEV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498816"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1181)
                     /locus_tag="VFV55_05755"
     CDS             complement(249..1181)
                     /locus_tag="VFV55_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX4943838.1"
                     /translation="MIRLALVAALITVPVHAFDLQGHRGARGLAPENTLPAWKKALEL
                     GVDTIECDMAITKDGVVVVHHDPYLNPDIARGPDGKWLEKRGPAISQLTYEELQKYDV
                     GRLKPGTDYARPFPDQVPVDGTRIPRLADLFDLVKKSGNTKVGFDCETKVGPDDRAAT
                     LAPEEFARRAIAEIRKHGMQDRIMVQSFDWSTLQVIQKEAPEIRTMYLSSPRTLKAAS
                     DGTPSPWLAGFAPEQHGGSVPRAVKAAGGRIWAPNQTFLTPAILAEARSLGITVIPWT
                     VNDPAMMNKLLDMGVDGIISDRPDLVQAEIGKRK"
     gene            complement(1217..4444)
                     /locus_tag="VFV55_05760"
     CDS             complement(1217..4444)
                     /locus_tag="VFV55_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4943839.1"
                     /translation="MFLSDFSIKRPVTTVVVIIGLMCLGLLALKNLRVNQIPDVEQPV
                     IVVNIPYPGASPETVEREIVNRVEKALQSIPQVYQIRSTSAESNAQIVIIFNFKKNMV
                     EASDEIRNAIASVRHKLPVEMREPVLRRIDPSAQPIMQLALSSTGQSHAEISRLAEDI
                     LADRFRGIDGVAVVNVNGSLRRELSVLLRAERLREYNISVTDVVNALRMQNTTAPVGR
                     VKGALDEQSIRLVGRIESPAEFEQIVLMRRGNEVVRLGQVASVADGFAELSGFSLRSG
                     HPNVGISVTRSRDASTVTVAAKIRKLVGEINKTLPPGTKLEVTQDGGEDAEASLRNVI
                     EALMFGAMLTVFVVYAFLNSWRSTMITALSLPTSVIAAFIAVWACGFTLNFMTLLGLS
                     LAIGVLIDDAIVVRENIVRHMERGEDRVTAARGGTAEIGMAVTATTFSIIAVFIPVAF
                     MGGGAGEWFRPFALTVATSVLVSLFISFTLDPMLSAYWGDPVGYQQQEKKGFRKTLAR
                     FNAWFDHQADRYGNVIAWALHHRRSMTLIAALSLAVALGLQATVGGSSFLPVSDVGIV
                     AIDVRTPSSASLEYAKLKVEKAAELARTMPEALATNSQVNASGGRVYVDIGKSTKRKR
                     SAGEIAVELRNLLTRIVGAEYVVLDDLNNGVRKPVQIQFSGPDSRRLLAITGDFMEKM
                     AKLPGAVDVGLSEQEPKDELKIELDRGLANSLGISVGDAAQALRVAFAGVEVGDWVDP
                     TGESRDVAVRLHPDDRVDAANIERLPIAVGGSSMMVPLDQIATITMGKGPAQIQHSDG
                     KRMIAVSANVQGRSAGEVTADAMKVAKQIEFPAGYGLELGGASRDQKEVFSEMATALV
                     MGIALMYLVLVMQFGSFTAPLPVMLSLPLSLIGVVLALLVTRGTLNLMSFIGVIMLMG
                     LVAKNAILLLDCARKEEAQGVAREDALMHAGRVRLRPILMTTFALIAGMLPVAIGLGE
                     GGEFYRPMAVAIIGGTITSTILTLLVVPTFYDSIEIARDRAFAKFHAREARWNAFAAF
                     VVTLVEALLVLVGIRAIYRLAKKGIARLAGRPGRGPRAVGPVAGD"
     gene            complement(4447..5598)
                     /locus_tag="VFV55_05765"
     CDS             complement(4447..5598)
                     /locus_tag="VFV55_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4943840.1"
                     /translation="MTHRHTLVLVLALALAACGKGADAPQKGADAAAGQPILLAAEDV
                     HTVRSSALSSGPTITGTVQPERRADLRAEVSAIVLQVLKENGDTVRRGDLLVRLDDTA
                     IRDGLASAESASRAASQAFQQAERQFQRMTTLRASGMASVQQLEDAEIRRNNTQSDFE
                     GAKTRVVLARQQLQRTEVRAPFDGIVSDRKVSAGDTAQTGKELLKVIDPASMRFEGLV
                     SADNIGNVKAGQGVIFRVNGYPEQEFTGKVRRVNPAANPTTRQVEVLVDFTGQKQPRL
                     AGLYAEGHVETDSATSLTLPATALVRDGDKASAWRVKDNKLQKVTLALGERDPRTGDF
                     VLKGGLSEGDLVIRHPGALLKDGQAVVASAPAATTKAAATGDAAVPARN"
     gene            complement(5740..>6793)
                     /locus_tag="VFV55_05770"
     CDS             complement(5740..>6793)
                     /locus_tag="VFV55_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943841.1"
                     /translation="PGEGITTTNWATSRGAVITNATSPRVITGLTNGSTFSFTINGRK
                     NGGPGGPGAPTQVAVPTLAGANWAVGAPLGTGRLNGIVIGNTALGVANVAVGSNGAIF
                     TSINGAATTTPVNPAAPADLNAVWYATAGFVAAGANGTILLSFDGTTWTAQPSGTTAT
                     LNGGTSIGSGAYVAVGAAGTILTSASGTSWLPASSGTTNDLRAATYGNGLYVVVGTNG
                     TILTSSDGATWTAATSGTTRDLRGVAYAALASTTTTTVTNLYVAVGAAGTVLTSSDGL
                     TWTVQAPVSVADLAAVTYGGQFVAVGKAGSIYTSPDAVTWQARTSNTTNDLAAVVRTL
                     SGYTAVGDSGTNVSTF"
BASE COUNT          996 a         2274 c         2386 g         1137 t
ORIGIN      
        1 gtaaccacga atcgaggaaa cggtctcgac cgggagcagc aacgatatgg accccagcag
       61 cccaggcgcc caccttggcc gacgggagat gattcgccct tgacacgggc cggctaatgg
      121 gtggcctcga ctttccgggc acccatccga cggtcacgat tgcgtgccca ggattgcgat
      181 cgacttcctc cgccgcctcg actcgtcagt attcgcgaac cggcactccg ggaattccgt
      241 gtagggccct actttcgctt cccgatttcc gcctgcacga ggtcgggtcg atcggaaatg
      301 atgccgtcga cgcccatgtc gagcagcttg ttcatcatcg ccggatcgtt caccgtccag
      361 gggatgacgg tgatgccgag cgaacgtgcc tcggcgagaa tcgcgggcgt gaggaaggtc
      421 tggttcggcg cccagatcct gccgccggca gccttgacgg ccctgggaac ggacccgccg
      481 tgctgctcgg gagcgaagcc cgcgagccag ggcgagggcg tcccgtcgct ggccgctttc
      541 agcgtccgcg gggaggagag gtacatcgtg cggatctccg gcgcttcctt ctggatcacc
      601 tgcagcgtgc tccagtcgaa ggactgcacc atgatcctgt cctgcatccc gtgcttgcgg
      661 atctccgcga tcgccctgcg cgcgaactct tcgggggcga gagtggcggc gcgatcgtcc
      721 ggcccgacct tggtctcgca atcgaaaccc actttcgtgt tgcccgactt cttcaccagg
      781 tcgaaaaggt ccgcgagcct cgggattcgc gttccgtcga cgggcacctg gtccgggaac
      841 ggccgcgcgt aatccgtacc gggcttgagc cggccgacgt cgtatttctg cagttcctcg
      901 taggtgagct ggctgatggc cgggccgcgc ttctcgagcc actttccgtc ggggccgcgt
      961 gcgatgtcgg ggttgaggta cgggtcgtga tggaccacga cgaccccgtc cttcgtgatc
     1021 gccatgtcgc attcgatcgt gtccacgccg agctccagcg ccttcttcca ggcgggcagc
     1081 gtgttctcgg gcgcgaggcc gcgggcgccg cggtggccct gcaggtcgaa ggcgtggacg
     1141 ggaacggtga ttagggcggc gacgagcgcg agtcggatca tggtcgtgac ggaggagggg
     1201 aaggcccgac tctacctcag tcgcccgcca ccggcccgac ggcgcgcggg ccgcgccccg
     1261 gccggccggc caggcgggcg attcccttct tcgccaggcg gtagatcgcc cggatcccca
     1321 cgagaaccag cagcgcctcg accagcgtga cgacgaacgc cgcgaaggca ttccagcggg
     1381 cctcgcgagc gtggaatttc gcgaacgcac ggtcccgcgc gatctcgatc gagtcataga
     1441 aggtcggcac cacgaggagc gtgaggatcg tcgaggttat cgtgccgccg atgatggcca
     1501 cggccatcgg gcggtagaat tcgccgcctt cgcccaggcc gatcgccacg ggcagcatgc
     1561 ccgcgatgag cgcgaaggtg gtcatgagga tgggccgcag ccggacgcgc ccggcgtgca
     1621 tcagcgcgtc ctcgcgcgcc acgccctgcg cctcttcctt gcgcgcgcag tcgagcagca
     1681 ggatcgcgtt cttcgccacc aggcccatca gcatgatcac gccgatgaag ctcatgaggt
     1741 tgagcgtccc gcgcgtcacc agcagcgcca gcaccacgcc gatgaggctg agcggcagcg
     1801 agagcatcac cggcagtggg gcggtgaacg agccgaactg catcaccagc accaggtaca
     1861 tgagggcgat gcccatgacc agcgcggtgg ccatctcgga gaagacctcc ttctggtcgc
     1921 gcgaggcgcc gcccagctcc aggccgtagc cggccgggaa ctcgatctgc ttcgcgacct
     1981 tcatcgcgtc ggcggtgact tcgccggccg agcggccctg cacgttggcc gacaccgcga
     2041 tcatgcgctt gccgtcggag tgctggatct gcgccgggcc cttgcccatg gtgatggtgg
     2101 cgatctggtc cagcggcacc atcatgctgc tgccgcccac ggcgatgggc aggcgctcga
     2161 tgttggccgc gtccacccgg tcgtccggat gcaggcgcac ggcgacgtcg cgcgactccc
     2221 cggtggggtc gacccagtcg cccacctcca cgccggcgaa tgcgacacgc agcgcctgcg
     2281 ccgcgtcgcc cacggagatg ccgagggagt tggccagccc gcggtcgagc tcgatcttca
     2341 gctcgtcctt gggttcctgc tcggaaaggc ccacgtcgac cgcgcccggc aacttcgcca
     2401 tcttctccat gaaatcgccg gtgatggcca ggaggcgccg cgaatccggc ccggagaact
     2461 ggatctgcac gggcttgcgc acgccgttgt tgaggtcgtc gagcaccacg tactcggcgc
     2521 cgacgattcg ggtcaggaga ttgcgcagtt ccaccgctat ctcgccggcg gagcgcttgc
     2581 gcttcgtgct cttgccgatg tccacgtaca cgcggccgcc gctcgcgttc acctggctgt
     2641 tggtggcgag cgcctccggc atcgtgcggg cgagttccgc ggccttctcc accttgagct
     2701 tcgcgtactc caggctcgcg ctcgacgggg tgcgcacgtc gatggccacg atgcccacgt
     2761 cggagacggg caggaagctc gaacccccga ccgtggcctg aaggcccagg gcgaccgcga
     2821 ggctcagcgc cgcgatgagg gtcatggagc gccggtggtg cagcgcccag gcgatcacgt
     2881 tgccgtagcg gtcggcctgg tggtcgaacc aggcgttgaa ccgcgcaagc gtcttcctga
     2941 agcccttctt ctcctgctgc tggtagccca ccgggtctcc ccagtaggcc gagagcatgg
     3001 ggtccagggt gaacgagatg aagaggctca cgagcacgga ggtggcgacg gtgagcgcga
     3061 agggccggaa ccactcgccc gcgccgccac ccatgaaggc caccgggatg aacaccgcga
     3121 tgatcgagaa cgtggtggcg gtcaccgcca tcccgatctc ggccgtgccg ccgcgcgcgg
     3181 ccgtcacgcg gtcctcgccg cgctccatgt ggcgcacgat gttctcgcgc accacgatgg
     3241 catcgtcgat cagcacgccg atggccagcg acaggccgag aagcgtcatg aagttgagcg
     3301 tgaagccgca ggcccacacg gcgatgaagg cggcgatcac ggaggtgggc agggacaggg
     3361 ccgtgatcat cgtggagcgc caggaattga ggaaggcgta caccacgaag acggtcagca
     3421 tcgcgccgaa catcagcgcc tcgatcacgt tccgcaagct cgcttccgcg tcctcgccgc
     3481 cgtcctgcgt gacctcgagc ttcgtgccgg gcggaagggt cttgttgatc tcgcccacca
     3541 gcttgcggat cttggccgcg acggtgacgg tgctcgcgtc gcgcgaacgc gtgaccgaga
     3601 tgccgacgtt ggggtgcccg ctgcgcaggc tgaagccgct cagctccgcg aagccgtccg
     3661 caacgctcgc cacctggccc agccgcacca cctcgttgcc gcggcgcatg agcacgatct
     3721 gctcgaactc cgccggcgac tcgatgcgcc cgaccaggcg gatgctctgc tcgtccagcg
     3781 cgcccttcac gcgcccgacc ggggcggtag tgttctgcat gcgcagcgcg ttgaccacgt
     3841 cggtgacgga gatgttgtac tcgcggagcc tttccgcgcg cagcagcacc gagagctcgc
     3901 ggcgcaacga cccgttcacg ttcaccaccg ccacgccgtc gatgccgcgg aacctgtcgg
     3961 ccaggatgtc ctcggccagg cgcgagatct cggcgtggga ttgccccgtg gacgaaagtg
     4021 cgagctgcat gatgggctgc gccgaagggt cgatgcgccg caggacgggc tcgcgcatct
     4081 ccaccggcag cttgtggcgc accgaggcga tcgcgttgcg gatctcgtcg gaggcctcca
     4141 ccatgttctt cttgaagttg aagatgatga cgatctgggc attgctctcg gcggacgtgg
     4201 agcggatctg gtagacctgc gggatgctct gcagggcctt ttcgacgcgg ttcacgatct
     4261 cgcgttccac ggtctcgggc gaggcgcccg gataggggat gttgacgacg atcaccggct
     4321 gctcgacatc cgggatctgg ttgacgcgca ggttcttgag cgcgagcagc cccaggcaca
     4381 tgaggccgat gatgacgacg acggtcgtca ccgggcgctt gatgctgaag tcggaaagga
     4441 acatggctaa ttcctcgcgg ggacggcggc gtcgcccgtg gcggcagcct tggtggtcgc
     4501 ggcgggtgct gacgccacca cggcctggcc gtccttgagc agcgcgcccg gatggcggat
     4561 caccaggtcg ccttcggaga gcccgccctt gagcacgaag tcgccggtgc gcggatcgcg
     4621 ctcgccgagg gcgagggtca ccttctgcag cttgttgtcc ttcacgcgcc aggccgatgc
     4681 cttgtcgccg tcgcgcacga gcgccgtggc gggcagggtg aggctcgtgg cgctgtcggt
     4741 ttcgacgtgg ccttccgcgt agaggccggc gagccgcggc tgcttctggc cggtgaagtc
     4801 caccagcacc tcgacctggc gcgtggtcgg gttggccgcg ggattgaccc gccgcacctt
     4861 cccggtgaac tcctgctcgg gatagccgtt cacccggaat atcacgccct gtccggcctt
     4921 cacgttgccg atgttgtcgg ccgacaccag gccctcgaag cgcatgctgg ccgggtcgat
     4981 caccttgagg agttccttgc cggtctgcgc ggtgtctccc gcggaaacct tgcggtcgct
     5041 cacgatgccg tcgaagggcg cgcgcacttc ggtgcgctgc agttgctggc gggcgagcac
     5101 cacgcgcgtc ttggcccctt cgaagtcgct ctgcgtgttg ttgcggcgga tctcggcatc
     5161 ctccagctgc tggacggagg ccatgccgga ggcgcgcagg gtggtcatgc gctggaactg
     5221 ccgctcggcc tgctggaagg cctgggatgc ggcgcggctg gccgactcgg cggaggccag
     5281 cccgtcgcgg atggcggtgt cgtcgaggcg cacgagcaag tcgccgcggc gcacggtgtc
     5341 gccgttctcc ttcagcacct gcaggacgat cgcggagact tccgcgcgca ggtccgcgcg
     5401 gcgttcgggc tggaccgtgc cggtgatcgt ggggcccgag gagagggcgc tgctcctgac
     5461 ggtatggaca tcctcggcgg cgaggaggat gggctgcccg gcggcagcgt cggcaccctt
     5521 ttggggggca tccgctccct tgccgcaagc ggcgagggcc agcgcgagga cgaggaccag
     5581 ggtatggcga tgcgtcatgt cgggtttccg gggttcctga ggatgcgaca ggttatgtcg
     5641 gcccccggac ggggaccacc acggtaggac gaactgcgga atcagggggg tgaattgtag
     5701 gcttcgtggt gccggcaagc gttaccgaac ctgtgattct caaaacgtcg agacgttcgt
     5761 ccccgagtcg cccacggcgg tgtaccccga gagcgtgcgc acgaccgccg ccaggtcgtt
     5821 ggtcgtgttc gaggttcgtg cctgccaggt caccgcgtcg gggctggtgt agatgcttcc
     5881 cgccttcccg accgccacga actgcccgcc gtaggtcacc gcggcaaggt ccgcgaccga
     5941 gaccggcgcc tgcaccgtcc aggtcaatcc gtcgctgctg gtgaggaccg tccccgccgc
     6001 gccgaccgcc acgtagaggt tggtcaccgt cgtcgtcgtg gtcgatgcca gcgccgcata
     6061 ggcgacgccg cgcaggtccc gggtcgtccc ggaggtggcg gcagtccagg tggccccgtc
     6121 gctgctcgtc aggatggtgc cgttcgtgcc gacgacgacg tagagaccgt tgccgtaggt
     6181 tgcggcacgc aggtcgttcg tggtccccga cgacgccggg agccagctcg ttccgctcgc
     6241 gctggtgagg atggtgccgg ccgcgccgac cgccacgtac gcgcccgacc cgatgctcgt
     6301 gccgccgttg agcgtggcgg tggtcccgct cggctgcgcg gtccacgtgg tgccgtcgaa
     6361 gctgagcagg atcgtgccgt tggcgccggc ggccacgaag cccgcggtgg cgtaccacac
     6421 ggcgttcagg tcggcgggcg cggcaggatt gaccggcgtt gtcgtcgcgg cgccgttgat
     6481 gctggtgaag atcgcgccgt tcgagcccac ggccacgttc gcgactccca gggcggtatt
     6541 gccgatgacg atgccgttga gccggccagt gcccagcggg gcccccacgg cccagttggc
     6601 gcccgcgagc gtcggcacgg cgacctgcgt cggcgcgccc ggcccgccgg ggccgccgtt
     6661 cttgcgaccg ttgatcgtga aggaaaaggt gctgccgttg gtgagtccgg tgatcacgcg
     6721 cggcgacgtg gcgttcgtga tcacggcgcc ccgggaagtc gcccagttcg tcgtggtgat
     6781 cccctcgccg ggg
//
LOCUS       DASUEV010000178         5459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_268264, whole genome shotgun sequence.
ACCESSION   DASUEV010000178 DASUEV010000000
VERSION     DASUEV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268264"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VFV55_05775"
     CDS             complement(<1..1379)
                     /locus_tag="VFV55_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943842.1"
                     /translation="MFGDAKSCKDWLNGLALTNIPQAQQFVLDALRSINRGEPGGLER
                     LKCLELMRDKIAFLQGEQRSRYFGKSLPLSSNDHAAWSIGRSLLEEMETGYRQCLAAV
                     AAEKGELAHHAPLMMQRVIRYLGAQMLFHAMIYRRFDPALWLRLHEQFAAAERDGVAD
                     ERVKDSLESEDGVSSIHGAYVQVVLTQAAYLSEMTAPQMDLLEALLRMWARKVHVLVD
                     APAGSTFPLAVDLARPIGARPLSASDRGPTHRVLDVEGLSKSIRRRLKGLQAGEEPVS
                     LGLPAEASSVDALAQLARLHKLWCEGAPPRPPAKIPDEKNAGLAFGLPEIHFFASGGA
                     AFEQPDKERELTRQEKDDLAVFGKVTERTQQLRLADYNFAIENWGVVDEMMGAWRLLR
                     PPTSSKGVAIGRILAMRLGEAAPFFLGMVQAIVQETDGRVVITVALFPGKPEPVAVRA
                     ADSRNRVGA"
     gene            complement(1426..1848)
                     /locus_tag="VFV55_05780"
     CDS             complement(1426..1848)
                     /locus_tag="VFV55_05780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX4943843.1"
                     /translation="MDCELCRGAGGAVLWQNDLCRVVAVDEPGLPGFIRVVLQRHVRE
                     MTDLSETQRNGLMAVVFAVEAHVRKSLEPDKMNLASLGNVTPHLHWHVIPRWRDDRHF
                     PGPVWAAARNDSPVPPARAAAAARMAATLPAALAALGG"
     gene            complement(1959..>5459)
                     /locus_tag="VFV55_05785"
     CDS             complement(1959..>5459)
                     /locus_tag="VFV55_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX4943844.1"
                     /translation="DAFEREFDETRKLRRRAMRRLSAITRKDNIAYDGLARVSHVTDA
                     TDWRVEYPFVVVSPDTEAECAPLVKALIDLGLTIIARGGGTGYTGGAIPLERLSAVVN
                     TEKLDWLGAVEQKALPGVDGLVPVIRVGAGAVTRRVMEAAEDAGLVFAVDPTSADASC
                     IGGNIAMNAGGKKAVLWGTAVDNLASWRMVTPDADWIEVERLDHNLGKIHDAGTARFR
                     IRRFEADGKTPKGEEILAIEGRRFRKPGLGKDVTDKVLGGLPGVQKEGCDGIITSATF
                     ILHRMPAHTRTVCLEFFGDVALAVPAIVEITKRVNGRDGVALAGLEHLDERYVKAVGY
                     ATKAKSHGRPRMVLIGDLVGDDENAVALAASEVVRIANARGGEGFIAASPEARKRFWM
                     ERARTAAIAKHTNAFKVNEDVVIPLDRLADYTNGVERLNVEYSIGNKLKLASALEEYL
                     AGDLPVETDGEAIRREDFLGDRPAQALELVRYVRGRWSYLLRNLDRKLGEVRADLGPL
                     GLTPLLAAQPDERTIFSLIQLHDLQVSWKRELRDPLRQVFAGRAFEPVMAAVQSTHAG
                     VLRGRVFIALHMHAGDGNVHTNIPVNSDDYEMLRTANEAVAHIMRFARELGGVVSGEH
                     GIGITKLEFLDKEQVEAFAAWKAKVDPEGRFNRGKLLPGADLSNAYTPSFALIGHESL
                     ILERSDIGSIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILALSLLTEAFLYEE
                     QTRRGVSLRHFDEFGDVADHCTVCHKCESPCPVDIDFGDVSIAMRGLLAKLGRKRFNP
                     GTAAAMFYLNATDPTTITIAKTLMIDWGYKAQRLAHGIARRFLVTRGQTRHPPATVGK
                     PALKAQVIHFINKPMPGNLPKKTARALLDIEDASVVPIIRDPAKVTEDSDAVFYFPGC
                     GSERLFGQVGLATQALLYHVGAQCVLPPGYLCCGYPQTASGQAAKGEEITIRNRILFH
                     RVANTLNYLDIRTVVVSCGTCMDALMDYEFDKIFPGCRLLDIHEYLMEKGVKVQGVSG
                     VRYMYHDPCHTPMKTHKPIEVVNELMGVPVALNDRCCGESGTLAISRPDVSTQVRFRK
                     EEEMVKGAQAARADGFSGEVKILTSCPSCLQGLSRYDNDAGTSADYIVVEMARHLLGE
                     RWMPEFVERANRGGIERVLL"
BASE COUNT          823 a         1870 c         1853 g          913 t
ORIGIN      
        1 tgcgcgccca cgcgatttcg cgagtccgcg gcacgcaccg caacgggctc gggcttgccg
       61 gggaacagcg ccaccgtgat cacgacgcgt ccgtccgtct cctgcacgat ggcctgcacc
      121 atgccgagga agaacggcgc cgcctctccc agccgcatgg cgaggatgcg cccgatggcc
      181 acgcccttgg aggacgtcgg cgggcgcagc aatcgccagg cgcccatcat ctcgtccacc
      241 acgccccagt tctcgatggc gaagttgtag tccgcgaggc gcaactgctg cgtgcgctcc
      301 gtgaccttgc cgaacaccgc gaggtcgtcc ttttcctggc gagtgagctc gcgctccttg
      361 tccggttgct cgaaagcggc cccgccgctc gcgaagaaat ggatctcggg aagcccgaac
      421 gcgaggcctg cgttcttctc gtccgggatc ttggccggcg ggcgcggcgg cgcgccctcg
      481 caccacagct tgtgcaggcg ggccagttgc gcgagggcat cgacggaact cgcctccgcg
      541 ggaagcccga ggctcaccgg ctcctcgccg gcctgcagcc ccttcaggcg ccgccggatg
      601 ctcttcgaca gcccctcgac atcgagcacg cgatgcgtgg gcccgcgatc gctggcactc
      661 aacgggcgtg caccgatggg cctggcgagg tcaaccgcaa gcggaaacgt ggagccggcc
      721 ggcgcgtcga ccagcacgtg caccttgcgc gcccacatgc gcagcagcgc ctcgaggagg
      781 tccatctgcg gagccgtcat ttccgacagg taggcggcct gcgtgagcac gacttgcacg
      841 taggcgccgt gaatgctgga cacgccgtcc tcgctctcga ggctgtcctt cacgcgctcg
      901 tcggcgaccc catcgcgctc ggcggccgcg aactgctcgt gcaagcgcag ccagagcgcc
      961 gggtcgaagc gccggtagat catcgcgtgg aaaagcatct gcgcgccgag gtagcggatg
     1021 acgcgctgca tcatcagtgg cgcgtggtgc gccagttcgc ccttttcggc cgcgaccgcc
     1081 gccaggcact gccggtagcc cgtttccatc tcctcgagca gcgagcggcc gatggaccac
     1141 gccgcgtggt cgttgctcga gagcgggagt gatttcccga agtagcggga acgctgctcg
     1201 ccctgcagga aggcgatctt gtcgcgcatg agctccaggc acttcaggcg ctcgaggccc
     1261 ccgggttcgc cgcggttgat cgagcgaagc gcatccagca cgaactgctg cgcctgcggg
     1321 atgttggtga gtgcaagccc gttcagccag tccttgcagc tcttggcatc gccgaacacg
     1381 aggccggctc cgggtcggct gggtgggttg gcggccattc gaccctcacc cgcccagggc
     1441 cgcgagcgcc gcgggcagcg tggcggccat gcgtgccgcc gccgccgcgc gggccggcgg
     1501 aaccggggaa tcgttcctgg cggccgccca caccggcccg gggaaatggc ggtcgtcgcg
     1561 ccagcgcggg atgacgtgcc agtgcaggtg cggtgtcacg ttgccgaggc tcgcgagatt
     1621 catcttgtcc ggttccagcg attttcttac atgggcctcc accgcgaaca ccacggccat
     1681 caggccgttt cgctgcgtct cggagaggtc ggtcatttca cgcacgtggc gttgcagcac
     1741 cacccggatg aaccccggga ggccgggttc gtccaccgcc accacccggc acaaatcatt
     1801 ttgccagagg accgcgccgc ccgcgccccg gcaaagttca caatccacag gcgggctcct
     1861 tccggacgtg gggcatggtt gtcccgggaa cgaggagagg ggaactgggg attgtccgcg
     1921 aggcccggcg cgccctgcaa ccgggtccgg cgcagcccct agagcaggac gcgttcaatg
     1981 cccccccggt tggcgcgctc gacgaactcc ggcatccagc gctcgccaag caggtggcgc
     2041 gccatctcca cgacgatgta atccgcgctc gtgcccgcgt cgttgtcgta tcgcgagagc
     2101 ccctgcaggc aggacgggca ggacgtgagg atcttcacct cgccggagaa gccgtcggcc
     2161 cgcgccgcct gcgccccctt gaccatctcc tcttccttgc ggaagcggac ctgcgtggag
     2221 acgtccggcc gggagatcgc gagcgtaccc gactcgccgc agcatcgatc gttgagggcg
     2281 accggcacgc ccatgagctc gttcaccacc tcgatcggct tgtgcgtctt catcggcgtg
     2341 tggcaggggt cgtggtacat gtagcgcacg ccgctcactc cctgcacctt tacgcccttt
     2401 tccatcaggt actcgtggat gtcgagcagc cggcagccgg ggaagatctt gtcgaactcg
     2461 tagtccatca gggcatccat gcacgtgccg cacgaaacca cgaccgtcct gatgtccagg
     2521 tagttgaggg tgttggccac ccggtggaag aggatgcggt tgcggatcgt gatttcctcg
     2581 cccttggccg cctgcccgct tgccgtctgc ggataaccgc agcacaggta gcccggcggc
     2641 aggacgcatt gcgcgccgac atggtaaagc agcgcctgcg tggcgagccc cacctgcccg
     2701 aacagccgct ccgatccgca acccgggaaa tagaacacgg catccgaatc ctccgtgacc
     2761 ttggccgggt cgcggatgat gggcaccacg gaggcgtcct cgatgtcgag gagcgcacgc
     2821 gccgtcttct tcgggaggtt gcccggcatc ggcttgttga tgaagtggat cacctgggcc
     2881 ttgagcgcgg gcttgcccac ggtggcgggc gggtggcgcg tctgcccgcg cgtcacgagg
     2941 aaacgccgcg cgatgccgtg cgcgaggcgc tgcgccttgt agccccagtc gatcatcagc
     3001 gtcttggcga tcgtgatggt cgtggggtcg gtcgcattga ggtagaacat cgcggccgcc
     3061 gttccgggat tgaagcgctt cctgcccagc ttcgcgagca ggccgcgcat cgcgatggag
     3121 acgtcgccga agtcgatgtc caccgggcac ggcgactcgc acttgtggca gaccgtgcaa
     3181 tggtcggcca cgtcgccgaa ttcgtcgaag tgcctcaggc tcacgccccg gcgcgtctgc
     3241 tcctcgtaaa ggaaagcctc ggtgagcagc gacagggcga gaatcttgtt cctcgggctg
     3301 tagaggaggt tcgcgcgcgg cacgtgggtc gagcacacgg gcttgcactt gccgcagcgc
     3361 aggcagtcct tcacggagtc cgcgatcgac ccgatgtcgc ttcgctcgag gatgagcgac
     3421 tcgtgcccga tgagcgcgaa ggatggcgtg taggcgttcg agaggtcggc cccggggaga
     3481 agcttgccgc ggttgaagcg gccctccgga tcgaccttcg ccttccaggc cgcgaaggct
     3541 tcgacttgtt ccttgtccag gaactcgagc ttggtgatgc cgatgccgtg ctcgccggag
     3601 accacgccac cgagttccct tgcgaaccgc atgatgtgcg ccaccgcctc gttggccgtg
     3661 cgcagcatct cgtagtcgtc cgagttgacc gggatgttgg tgtgcacgtt gccgtcgccc
     3721 gcgtgcatgt gcagggcaat gaacacgcgg ccgcgcagca cgccggcgtg cgtcgactgg
     3781 acggccgcca tcacgggctc gaaggcgcgg ccggcgaaga cctgccggag gggatcgcgc
     3841 agctcgcgct tccacgacac ctgcaggtcg tgcagctgga tcagggagaa gatcgtccgc
     3901 tcgtccggct gcgctgccaa caggggggtg agcccaagag ggcccaggtc tgcgcgcacc
     3961 tcgcccagct tgcggtcgag gttgcgcagg aggtatgacc agcgccctct cacgtagcgc
     4021 accagttcga gggcctgtgc gggacggtcg ccgaggaagt cctcgcgccg gatagcctcg
     4081 ccgtcggtct cgacgggcag gtcgcccgcg aggtattcct cgagcgcgga cgcgagcttc
     4141 agcttgttgc cgatcgagta ttcgacgttc aggcgctcga cgccgttggt gtagtcggcc
     4201 agccggtcga gcgggatcac cacgtcctcg ttcaccttga aggcgttggt gtgcttggca
     4261 atggcggcgg tgcgggcgcg ctccatccag aagcgcttcc tcgcctccgg actcgcggca
     4321 atgaagcctt ctccgccgcg cgcgttggcg atgcgaacga cctccgacgc ggccagagcc
     4381 actgcgttct cgtcgtcgcc cacgaggtcg ccgatgagca ccatgcgcgg ccgcccgtgg
     4441 gacttcgcct tcgtcgcgta gcccacggcc ttcacgtagc gctcgtcgag atgctcgagc
     4501 ccggcgagcg cgacgccatc gcggccgttt acccgcttcg tgatctcgac gatggccggc
     4561 accgcgagcg ccacgtcgcc gaagaactcc aggcacaccg tgcgcgtgtg cgcgggcatg
     4621 cggtgcagga tgaaggtggc cgaggtgatg atcccgtcgc agccttcctt ctgcaccccg
     4681 ggcaggccgc cgagcacctt gtccgtgacg tcctttccca ggccgggctt gcggaagcgc
     4741 cggccctcga tcgccaggat ttcctcgccc ttcggggtct ttccgtccgc ctcgaagcgg
     4801 cggatgcgga agcgcgcggt gccggcgtcg tggatcttgc ccaggttgtg gtcgaggcgc
     4861 tcgacctcga tccagtccgc gtcgggggtg accatgcgcc acgaggccag gttgtcgacg
     4921 gccgtgcccc agagcaccgc cttcttgccg ccggcattca tcgcgatgtt tccaccgatg
     4981 caggaagcgt cggccgacgt gggatcgaca gcgaaaacga ggccggcatc ctcggcggcc
     5041 tccatgactc ggcgagtgac cgcgcccgcg cccacccgga ttacggggac caatccgtcc
     5101 acgcccggca gcgccttttg ctccaccgca cccagccagt cgagcttctc ggtgttcacg
     5161 accgcggaaa ggcgctcgag cgggatggcg ccgcccgtgt agccggtgcc gccgccgcgc
     5221 gcgatgatgg tgagccccag gtcgatcagc gccttcacga gcggcgcgca ttcggcctcc
     5281 gtgtccggcg agaccacgac gaagggatat tccacgcgcc agtccgtggc gtcggtcacg
     5341 tgcgagacgc gcgcgagacc gtcgtaggcg atgttgtcct tgcgggtgat ggcggagagc
     5401 cggcgcatcg ccctgcggcg aagctttctc gtctcgtcga actcccgctc gaaggcgtc
//
LOCUS       DASUEV010000179         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1176283, whole genome shotgun sequence.
ACCESSION   DASUEV010000179 DASUEV010000000
VERSION     DASUEV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176283"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="tgt"
                     /locus_tag="VFV55_05790"
     CDS             complement(<1..1041)
                     /gene="tgt"
                     /locus_tag="VFV55_05790"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX4943845.1"
                     /translation="MKFDLLAVDGAARRGTLALAHGTVETPAFMPVGTYGTVKAMSPA
                     ELAGIGTRIVLGNTFHLWLRPGLEVIAAHGGLHRFMGWDKPILTDSGGFQVYSLGPLR
                     KVSEEGVAFRSPVDGDKLFLTPEESMRIQRVFDSDVAMIFDECTAWPATRDEAADSMR
                     LSLRWAARSKAAHEGNANALFGIVQGGMHEDLRDESREALEAIGFDGYAIGGVSVGEP
                     KEEMARIVAHTTPRLPPNRPRYLMGVGTPEDLVDAVAAGVDLFDCVLPTRNARNGWLF
                     TRFGDLKIKNARHRADTQPLDATCGCYACRNFTRAYLHHLHRANEILGSRLNTIHNLT
                     YYQVLMGEMREAI"
     gene            complement(1038..1694)
                     /locus_tag="VFV55_05795"
     CDS             complement(1038..1694)
                     /locus_tag="VFV55_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX4943846.1"
                     /translation="MALVFYHGHGSPYSWRVWLALEHLGIPYELKVLSFAEKDTSKPE
                     FVAINPRHQVPTIVDDGFALWESLAILEYLDERGGTGARRLYPGTAQERARIRRLVRE
                     DEEHLDRKGLTPVYDELYLKPAGTPPDEARLEEGRSRLREELGYFGRELRGPFLAGET
                     PTAADFVLYPDIAYVKRIGVKKPASRLEGILPGPIAQWASRIEALPYFEKTYPPHWRS
                     "
     gene            complement(1847..>2344)
                     /locus_tag="VFV55_05800"
     CDS             complement(1847..>2344)
                     /locus_tag="VFV55_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX4943847.1"
                     /translation="AMEAVGRAAGSVVIEVRRQFRDIKGIMEGTAKPEYGVAVDMLTK
                     AAIKEMIVPSLLPVLSPVAVALIVGFLMGKDAGVQALGGMLMGTIVTGLFVAISMCTG
                     GGAWDNAKKYIEDGHYGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALL
                     IVPLL"
BASE COUNT          363 a          785 c          817 g          379 t
ORIGIN      
        1 gatggcttcg cgcatttcgc ccatcagcac ctggtagtag gtcaggttgt ggatcgtgtt
       61 gagccgcgag ccgaggatct cgttggcacg gtgcaggtgg tgaaggtagg cgcgcgtgaa
      121 gttccggcac gcatagcagc cgcaggtcgc gtcgaggggt tgcgtgtcgg cgcggtgccg
      181 cgcgttcttg atcttcaggt cgccgaagcg cgtgaacagc cagccattgc gggcgttgcg
      241 ggtcggcagg acgcagtcga agagatcgac gccggccgcc acggcgtcca cgagatcctc
      301 cggcgttccc acgcccatga ggtagcgcgg gcggttcggg ggcagcctcg gcgtcgtgtg
      361 cgcgacgatg cgcgccatct cctccttggg ctcgcccacg gagacgccac cgatcgcgta
      421 gccgtcgaag ccgatcgcct ccagcgcctc gcgcgactcg tcgcgcaggt cctcgtgcat
      481 gccgccctgc acgatgccga agagcgcgtt ggcatttccc tcgtgggctg ccttcgagcg
      541 cgccgcccag cgaagcgaga gccgcatcga atcggccgcc tcgtcgcgcg tggccggcca
      601 ggcggtgcac tcgtcgaaga tcatcgcgac gtcggaatcg aacacgcgct ggatgcgcat
      661 cgattcctcc ggcgtgagga agagcttgtc gccgtccacg ggcgagcgga aggccacgcc
      721 ctcctcgctc accttgcgca gcggccccag gctgtagacc tggaaaccgc cggaatcggt
      781 gaggatgggc ttgtcccatc ccatgaagcg gtgcaggccg ccgtgcgcgg cgatgacctc
      841 cagcccgggt cgcagccaca ggtggaaggt gttgcccagc acgatgcggg tgccgatccc
      901 ggcgagctcc gcgggcgaca tggccttcac cgtgccgtag gtacccacgg gcatgaaggc
      961 gggcgtctcc acggtgccat gggccagcgc gagcgtgccg cggcgcgcgg cgccgtccac
     1021 cgcgagcagg tcgaacttca cgagcgccag tgcggcgggt aggtcttctc gaagtagggc
     1081 aacgcctcga tgcgggaagc ccactgcgcg atcggcccgg gaagaatccc ctcgagccgc
     1141 gatgcgggct tcttcacccc gatgcgcttc acgtacgcga tgtccggata gaggacgaag
     1201 tcggccgccg tcggcgtctc gccggcgagg aacggcccgc gcaactcgcg cccgaagtag
     1261 ccgagctcct cccggaggcg gcttcgccct tcctcgagac gggcttcatc cgggggcgta
     1321 cccgccggct tgaggtagag ctcgtcgtag acgggcgtga ggcccttcct gtccaggtgc
     1381 tcctcgtcct cgcgcacgag gcggcggatg cgggcgcgct cctgcgccgt gccggggtac
     1441 agccgccgcg cgcccgtgcc gccgcgctcg tcgaggtact cgaggatcgc cagcgattcc
     1501 cagagcgcga agccgtcgtc cacgatcgtg ggcacctggt ggcgcggatt gatggcgacg
     1561 aactccggct tcgaggtgtc cttctccgcg aaggacagca ccttgagctc gtagggaatg
     1621 ccgaggtgct cgagggcgag ccagacccgc catgaatagg gcgagccgtg cccgtgatag
     1681 aagacaagtg ccatgggaaa ttcctttcgt tcgcgtgcca ggtgggagtc tagcgaatgc
     1741 gagggggtgg ggttgaacgc ggaggaagca gaggcctctt cgccaagaac gcgtggacaa
     1801 cgaaagggcc gcccgaaggc ggcccgtcca tgccggcgag cgccgcctac agcagcggaa
     1861 cgatgagcag tgcgacgatg ttgatgatct tgatgagcgg gttgaccgcc ggtccggcgg
     1921 tgtccttgta ggggtcgccc accgtgtcgc cggtcaccgc ggccttgtgg gcgtcggagc
     1981 ccttgccgcc gtagtggccg tcctcgatgt acttcttggc gttgtcccac gcgccgccgc
     2041 ccgtgcacat cgagatcgcg acgaagaggc cggtcacgat cgttcccatg agcatgccgc
     2101 cgagcgcctg cacgcccgcg tccttgccca tcaggaagcc gacgatgagg gccacggcga
     2161 ccggcgacag caccggcagc agcgacggca cgatcatttc cttgatcgcc gccttggtca
     2221 gcatgtcgac ggccacaccg tactcgggct tggccgtgcc ttccatgatg cccttgatgt
     2281 ccctgaattg ccggcgcacc tcgatcacca ccgagcccgc ggcgcgtccc accgcttcca
     2341 tcgc
//
LOCUS       DASUEV010000180        25802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_268922, whole genome shotgun sequence.
ACCESSION   DASUEV010000180 DASUEV010000000
VERSION     DASUEV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 25802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25802
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268922"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /gene="nuoL"
                     /locus_tag="VFV55_05805"
     CDS             <1..1596
                     /gene="nuoL"
                     /locus_tag="VFV55_05805"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX4943848.1"
                     /translation="LMLVMADNFVQLFFGWEAVGLVSYLLIGFWFKRPTAIYANLKAF
                     LVNRVGDFGFLLGIGFVFAYFGTFGYAEVFARAPDLARTAIELVPGHPVALGTVICIG
                     LFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLFELSQAA
                     LSFVIVIGAITGFFMALVAIVQYDIKRVVAYSTLSQLGYMTMALGAAAYPVAIFHLMT
                     HAFFKAVLFLGAGSVIIALHHEQDMRKMGGLRKYMPITYLTVLVGALANAGLPPFAGF
                     FSKDSIIEALHLSQTPGAGFAWALAMAGVFVGGFYSFRLVFFAFHGKERFELHDDHDH
                     GGHGDGHGARPHESPWVVTVPLVLLAIPSAVIGWVTIEPMLYGGWFGESIAASGTMKE
                     MAREFHGAGAMVLHAFGTLPFWLALGGAGLAGFLYLARPDLPAVIKARSGVLATILER
                     KYGFDEFNDWFFAGGARGLGQGLFAWGDRTVIDGVMVNGTARLIGWFAGFARKVQTGY
                     VYHYAFTMIIGVFALLTFWYSRI"
     gene            1606..3093
                     /locus_tag="VFV55_05810"
     CDS             1606..3093
                     /locus_tag="VFV55_05810"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX4943849.1"
                     /translation="MAQGLPILSVVTWLPIAFGVAVLAIGSDRSPAAARWTALAGSVL
                     GFLAGVPLWTGFDVAAGGAMQFEELAPWIPLFHVNYHLGADGISVPLILLNSFMTVLV
                     VIAHWEAITDKVGQYLAAFLIMSGLINGTFAALDAILFYVFFEAMLIPMFLIIGVWGG
                     PNRVYAAVKFFLYTLLGSLLMLASFIYLYLQTNGSFEILDFHKVPLSMTAQVLVFLAM
                     FMSFAVKVPMWPVHTWLPDAHVEAPTGGSVILAAITLKIGAYGFLRFNLPIAPDASHA
                     LAGFMITLSLIAVVYIGLVALVQSDMKKLIAYSSISHMGFVTLGFFIFDADGMLGGLM
                     QMVSHGFVSAAMFLCVGVLYDRVHSRNIADYGGVANTMPVFAAFFVLFAMANSGLPAT
                     SGFVGEFLVIMGSVKVNFWYAFLAGTTLVFGAAYTLWMVKRVIYGEVANEHVAALRDV
                     NAREVVFLVLLAACVLAMGVYPKPFADVMNPTLHELLRHVTTTKL"
     gene            3090..4544
                     /gene="nuoN"
                     /locus_tag="VFV55_05815"
     CDS             3090..4544
                     /gene="nuoN"
                     /locus_tag="VFV55_05815"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX4943850.1"
                     /translation="MNTATFSLATLAPAIPEIALLALVSALLVIDLFVAENEKHVTFW
                     LAIGSLLGCAALSGMMAGNASAVTFQGMFVADPMSQVLKVAVLLAVAATLVYSRAYLQ
                     AREMLRGEFLSLALFAALGMMVMISAHHFLTLYLGLELLALSLYSMVALERDSARASE
                     AAMKYFVLGALASGMLLYGMSMLYGATGSLDIDRVANALGSAQSSQALAVFGLVFVVA
                     GTAFKLGAVPFHMWIPDVYQGAATPATLFIGAAPKIAAFAFVMRLLAHGLGGMAGDWQ
                     QMLAILAVGSIGLGNLVAIAQTNLKRMLAYSTISHMGFLLLGILAGTSNGYSSAMFYV
                     ITYVLMTLGAFGMILILSRAGFEAEELDDFRGLNRRSRWYAFLMLVIVFSLAGIPPAV
                     GFLAKLAVLQAAFEAGYGWLVVFAVLMSVVGAFYYLRLVKLMYMDEPAGEITIAPRLD
                     MRVLLSANAIAVLALGIVPAPLMDLCARAITASL"
     gene            4555..5121
                     /locus_tag="VFV55_05820"
     CDS             4555..5121
                     /locus_tag="VFV55_05820"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX4943851.1"
                     /translation="MKLPPDPTGDFTEATVSTKLAYDGGFLKLRRDEVRLPGGACAWR
                     EYVEHPGAVMMMAFLDDETILLERQYRYPLRRHFIELPAGKMEAGEPPLATARRELVE
                     ECGYEAADWWHMVTLHPSVGYADEVIELYGARDLRFVGACLDPGEHLEVLPARIEDAL
                     GWIGAGLVTDTKTMLGLLWWARFGRKAA"
     gene            5217..6707
                     /gene="glpD"
                     /locus_tag="VFV55_05825"
     CDS             5217..6707
                     /gene="glpD"
                     /locus_tag="VFV55_05825"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX4943852.1"
                     /translation="MEKYDLLVIGGGINGAGIARDAAGRGLKVLLVEMADLASATSGW
                     SSKLIHGGLRYLEYYEFRLVREALEEREVLLHAAPHIVRPMRFVLPHDRSMRPVWMIR
                     IGLWMYDHLGKRVSLPGSQTVAFPHVEYSAGLKSELRSAIVYSDCRVDDARLTVLTAM
                     SARDKGATILTRTKFVAARRENGAWQAELRDMRSGAARTITAAAVVNAAGPWVSSVLD
                     GVPHRATGAKVRLVKGSHIVVPKVHSLGHACLLQNPDKRVIFVIPFEGKYSLIGTTDV
                     PVETPEEAVDITAQETDYLIEAANRFLAKPISKADVVWTFAGVRPLYDDGKSDPSSIT
                     RDYVLELDDEGGKAPVLSLFGGKITTYRCLAETALQKLSPYLPPMGPLWTKESFLPGG
                     DMPDYNAFRDAIFDRYKGFQRDLLDGVVRRHGSRTATVLGDIHKPEELGTNFGAGLTA
                     REIDCLIADEWAETADDILWRRTKCGLHMDAAQRAAVESYIAGRKP"
     gene            7027..7821
                     /locus_tag="VFV55_05830"
     CDS             7027..7821
                     /locus_tag="VFV55_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX4943853.1"
                     /translation="MKELVEFPVKTRKAVRGVLTDIDDTLTTDGRLHAVAYAALERLH
                     EAGLLVIPVTGRPAGWCDHIARLWPVDAVVGENGAFWFRYDAKLRKLVKRYVIRDVER
                     LSRVQRLGEIAVRILEGVPGCAIASDQPYREADIAIDFCEDVRPLGRDAVARIVAIME
                     SEGLTAKVSSIHVNGWFGGYDKLSTTRLALAEDFGVDVERQKSRFVFVGDSPNDQPMF
                     AFFPNAVGVANIRKMADLMHILPRYVTSAPGSVGFAQVAAALLAAR"
     gene            complement(7822..10074)
                     /locus_tag="VFV55_05835"
     CDS             complement(7822..10074)
                     /locus_tag="VFV55_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4943854.1"
                     /translation="MPTEVFKRIVESLRTAVALADPKGVVAFANPAFAQLCGDPGHGV
                     SAASLASFFHKDDARRVRQNIARIAEGKTASALLDVRVASGGAWVQLALQPALDSRDK
                     PAGIVAVLHDIAGQRETESALNLTTARLMALVEASPNAALIENAAGEVELVNEAFCRL
                     LGLASAPQSLLGLPAIDVIGRSAIVDARALARAHRKPDAPASIAFQPADNPPATLERQ
                     PLLVDEEPAGALWSSRAASVESPAEADGGSSEIALIEKIGAELSVALEGLSAISIRAQ
                     QMDFDPALVQQFHDIRASTETALVAIGDLVDFSKVEGGIELRRAGFRLRPAVADLVKR
                     TAHAAEECRCHLRVKVEQDVPDHLEGDVERLQLLLRNLLESAFAAYPGAEVALAISPE
                     YTTASGIHLSFAVTASSGGSERPAPRAAADPGMGVAVARFMVAAMGGKLEAGSRPPEP
                     LYSFTIEFPVLPLTTPRRPAYVTLVGMSVLVVSANATQRHELSNLLRGWRMTPLEADN
                     AAMAIALLERMHEEGSPVPLMLASDRLAPQDGFLLAFRVKNHPALQNTLVVMLATEGK
                     PGDAIACRENGISAYLRYPLAAKQLNDAIVAVTGASVDSDETPTLVTRHSLREQRKGA
                     TILLVDGHRDSHMLAAHILRKRDSSLVVASDLAEAIASLDQDVYDIVFVDTALEGLDG
                     AGAAAMLRSHIKRDPQATRLYATSVEHSPAFRTARMAEGFDGTLAKPFRRDNLLAVLA
                     AIGKLPDETG"
     gene            complement(10095..11678)
                     /gene="pgi"
                     /locus_tag="VFV55_05840"
     CDS             complement(10095..11678)
                     /gene="pgi"
                     /locus_tag="VFV55_05840"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX4943855.1"
                     /translation="MPSPTPTPAWQALEAHRDAIAALRVDELFAADPGRGPALTFACA
                     GLAVDFSKQRITPETLERLVTLARERDMHGAIERLFTGQHVNVTEDRPALHTALRGDE
                     KVAANGENVLAHVQRCRERMRVLSTTVRDGTWKGAKGGRIRHVLSLGIGGSYLGPRLV
                     VEALAARTDGPEVRFLANIDPAALDDALAGLDPASTLVIIASKTFATQETMANAAAAR
                     DWITASLGEGAVAKHMVAATANAAEAARFGLPECNVLPFGDWVGGRYSVWSSVGLPAA
                     IALGMPAFERFLAGAHAVDLEFRSAPLERNVPVLLALAGVWNRDFLDIPVHLVLPYAQ
                     RLASLPGYLQQLEMESNGKRVDVHGAPVTCPTCPAIFGEVGTLGQHAFYQWLHQGTDA
                     ASCDFVVVARPMGSREAEHRVLLANALAQSEALMTGRSTGSPHRDCPGNRPSTTLVLP
                     CLEPAALGALIALYEHKVYAQSVLWGVNAFDQFGVELGKAIAGRVLPAFADPASALHP
                     ATSHLLGVIRKAAQGGQAR"
     gene            complement(11741..12631)
                     /locus_tag="VFV55_05845"
     CDS             complement(11741..12631)
                     /locus_tag="VFV55_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4943856.1"
                     /translation="MSDTTGNSMTWLALALVLTAALIHASWNFLLKKSGGGPGLITAA
                     CLGSVVVYAPFVAGAAWVQHYGFRPVHLALMFTSGMIHTAYFLLLDRAYRSGGDLSIV
                     YPLARATGPLLTIVVAVAVLGERPGPTALAGAVLIVISALVLTGNPLTWHKSEARHAV
                     GFALLTGFTISMYTICDKASVAAWLVPPLVYDWGCNFFRSLVLVPVTQKRWPGSIDKA
                     WRERRKTVIAIAVLSPLSYILVLTAMVFTPVSLVAPAREVSILFAALMGAYFLKEGDL
                     TRRLVAAAGMALGVAGLTVG"
     gene            complement(12663..13367)
                     /locus_tag="VFV55_05850"
     CDS             complement(12663..13367)
                     /locus_tag="VFV55_05850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX4943857.1"
                     /translation="MSANTVELVKDELDLMRRLLPLAGARVADLGCGAADMARRLVEG
                     GLVASVSALEVDQVQHAKNLAGPRPAGLEFFLAGADAIPFRDANFDIALMFKSLHHVP
                     VERLNAALAEIRRVLVPGGLLYVSEPVFAGPFNEIVRLFHDEEAVRAAALAALRRAAA
                     SGALEQVEERQFDVPLAFRDFDDFFDRIVRVTHSDHTLVGERLAEVRRRFEGHMGPGG
                     ARFVRPMRVNLMRRPV"
     gene            complement(13364..14164)
                     /locus_tag="VFV55_05855"
     CDS             complement(13364..14164)
                     /locus_tag="VFV55_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX4943858.1"
                     /translation="MMDRSFLFVPGNRPERFDKAYGAGAHAVIVDLEDAVAPADKAGA
                     RESARAWLSAAKPVWLRINGPESEWFAADLDLVGLPGIRGVMLPKAEDAAAIGEIRAR
                     AGSGVRIIALIESALGLWRAEALASAPGVERLAFGSVDFQLDTGIAGDREELLFARSH
                     LVLASRVAGRLAPVDGVTVAIDDEALLREDVERARRLGFGGKLCIHPKQVAGVNAGFV
                     PPEAELAWARRVLAAAEAGGNNAVRLDGKLIDLPIIDRARALLAQAAA"
     gene            complement(14161..15384)
                     /locus_tag="VFV55_05860"
     CDS             complement(14161..15384)
                     /locus_tag="VFV55_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4943859.1"
                     /translation="MRSTLFPLAATLAVQLLVSMAVVTVPVLAPAAAAELGVSAGYVG
                     FFIALVYGASMAASLVSGTLVQRFGAIRVSQLCLVSCAAGLALTATGGAAFLVAAAIL
                     LGFGYGPVTPASSHVLARTTPPGMMSLVFSLKQTGVPLGGALAGAVVPALVLVAGWRA
                     AALAVGAACLATLLLAQPLRASLDADRDPTRRLSVSGLAGPLRYTLGNPELRRLAICS
                     FFFASLQLCLITYLVTHLTANLGFSLVEAGLMLTVAQGGGVVARIAWGALADRWGRPM
                     RLLGLVAIAMGLAAIATAQFTAAWPAAAVAFVCAAFGATAIGWNGVYLAQIARRAPPG
                     KAGDATGGSLVFTYGGVLFGPPAFAFLVESGTRYSTAFMIVAVPAVACGLWLLWQDGF
                     RNEPGRETPGNLENG"
     gene            complement(15377..16147)
                     /locus_tag="VFV55_05865"
     CDS             complement(15377..16147)
                     /locus_tag="VFV55_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4943860.1"
                     /translation="MSARSPFPLVARQPQYALVAQRLLDDITGGRYPVGSLLPTEFEL
                     CTQFGVSRHTVREAIRRLHEHGLVTRQRGIGTSVKATRVDSRYVQSTASISDLPQYVA
                     DTRLVTTAAADVIADEFLAELLRCPAGQRWVKVTGFRYAGKEKLPMALTEIYINAAYG
                     GVRKLIGTLKVPVYTLIEKQYGITIVEVKQEIRAATIGAADAKRLSVKPGSAGLVVTR
                     RYFGDNDRPIEVAVNLHPADRFSYSMSMRLQVPAAADA"
     gene            complement(16144..17334)
                     /locus_tag="VFV55_05870"
     CDS             complement(16144..17334)
                     /locus_tag="VFV55_05870"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4943861.1"
                     /translation="MSLPLQGVRIVAVEQYGAGPFGTQHLADLGAEVIKIENPAEGGD
                     VGRLVGPYYFGPGDSHFYEAFNRNKKSLTLNLKNPEARGVLADLVKSADATFDNLRGD
                     LAGKLRLTYADLSVHNPRIVCGHLSAYGRSGSRASWPGYDYLMQAEAGYLSVTGEPDG
                     PPARFGLSIVDLMTGVTAVLGLLAGIIEARATGRGRDIDVSLFDVALTNLNYPAAWYL
                     NEGAMIGREPRGAHPSLTPSQLYRTKDGWIFVMCNKEKFWGVFAEAVGKPEWATGADY
                     GNYKSRLANRDRLTRDMDEVLSTATTAEWIGRLAGRVPVAPVYDVAQALENPFVKEQG
                     RIMEAPHPARGTIRTIACPIRCPGEAEKVGIAPTLGEHTREILGALGYDDARIDHLSQ
                     AGAL"
     gene            complement(17331..18485)
                     /locus_tag="VFV55_05875"
     CDS             complement(17331..18485)
                     /locus_tag="VFV55_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4943862.1"
                     /translation="MTPEQLEMEQMVLDSVDKFLEAEVRPNAHRLEKADEYPQEIVDK
                     MKELGLFGCIIDPEYGGLGLSTSTYAKIIERMAAVWMSVSGIINSHLIMAAAVQRSGT
                     EAQKRHYLPKFATGELRGGIGLTEPDAGTDLQAIRTVAVRDGDDYVVNGAKTWITNSM
                     YGNTIALLVKTDPQAKPRHKGMSLFIAEKGPGFKVARKLEKLGYRGIDTCELVFENYR
                     IPADRLIGGVEGKGLQQVLSGLELGRINVAARGVGVAREALKEAVAYSQTRKTFGKPI
                     CEHQAIQLKLGEMATRVEASRLLVESAARAYDRGERCDMEAGMAKYFATESAMENALE
                     CMRIHGGYGYSKEYNVERYFRDAPLLLIGEGTNEMQRIIIAKQLIERNPA"
     gene            complement(18522..19961)
                     /locus_tag="VFV55_05880"
     CDS             complement(18522..19961)
                     /locus_tag="VFV55_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX4943863.1"
                     /translation="MTNMAIPVGTAPEAAARGATIAETLAAFAESLRPEAIPAAVLER
                     ARYLILDAVGIAHASTRYEFAHRSLSAMTDLSAGLGDMPVIGLPARLQLRDAMLMNGI
                     LVHGLDFDDTHVQGVIHSTAACFPSALGVAAHAGLDGQDLLAAYVVGMEAGTRLGSVA
                     KGAFHQIGFHPTGLVGAFSATLIAGRLFGLNAAQLAMAQGIALSAASGSLEFLNDGAW
                     TKRMHPGWAGVAGFTAASLARHGFVGPREAYEGRFGLFASHLGQYAKDIDLSLATAGL
                     GETWEVARVAVKPIPACHFTHACADAAAILRDKHGLKVSDIRSVRALVPKEVVKTVCE
                     PVATKKRPQNSYDAQFSIPYIVATALARGSFGLAHLEDAALADAEVLALAQRVEYEAD
                     PASPFPKYYSGEVIVTTNDGRELRHREEMNRGCVDRPITNGEIERKFMANARLAVSAS
                     RAEEIRDLVLAIGPGLDARDLAEGLAARG"
     gene            complement(19983..21251)
                     /locus_tag="VFV55_05885"
     CDS             complement(19983..21251)
                     /locus_tag="VFV55_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX4943864.1"
                     /translation="MLMILAIVLMLALLAIGSHVAVSLGLVTTGLILMIDGVPMTVIA
                     QTAFKAVNSYPLMAVPMFILAGNLMMRGNIAGLMIDLIGSIVRAVQGGLAISVMIACV
                     FFAAISGSSVGSAAAIGAATVEGLKREDYPARFSAGIVAVGGTLGLMIPPSLGFILIG
                     SIVGLPVDRLFIAGVMPGLMEATLLLTAVAWMSRHYGYGTTTARADWGGFARRLPKAG
                     AALLMPVLVIGSIYTGYLTPTEVSAFAAVYAAILCILVYRSVTVGGVWTVLRDSVLQT
                     TMIFAVVMGGSLVGFVLARMGISASVVQYITSINMSAWQFLLVANLVLLVLGMFLDGV
                     AMIVLTSPLLFPVATALGINPIHFAVVMVANVEIATLTPPIGLNLFVMSGIAKLPVHE
                     VVRGVLPFYFVRLTGLMLITYIPAISLVLL"
     gene            complement(21267..21812)
                     /locus_tag="VFV55_05890"
     CDS             complement(21267..21812)
                     /locus_tag="VFV55_05890"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX4943865.1"
                     /translation="MSKPDRATAPLRGWWDWFESGPVLNFSALLFLASTLVMLSEGLN
                     RSFRDVSFFWAEESVRYLMIWAFFLTLGVAGRHGHHIRTEMLVDAMPPGIRRAMHFAS
                     VVAGIAFSAVLFASSIPQLIRYYTMGMVSESNLDIPQWIVFLAMPIGAALLGGYYMRC
                     FVVLLRRGDPFVSAGPTGSEL"
     gene            complement(21814..22824)
                     /gene="dctP"
                     /locus_tag="VFV55_05895"
     CDS             complement(21814..22824)
                     /gene="dctP"
                     /locus_tag="VFV55_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX4943866.1"
                     /translation="MTRGPAFRAATAAIAAAIALAAAPALRAAELVLPSEVATTHWKT
                     GYMNQFAEEVKKRTGGALVVKVFPAGQLYNDQDAMAALGTGAVHMVWPVAVRLETIDP
                     RTGIINLPFVLSDELMTNRCFAEDLTKLMSSYVEPRKLQVLGFLRTSDLLFVFKDRDV
                     QKLEDLKGAKVRVTGGKVFLDTMRSLNTSPVSMAASEMSTALSQGAIDGVFTSPAGWA
                     EMIGITAKYAWYVPGMSVATYAVVVDKAWLDGLPAVQKKAVTDTIAEIGLRQWAEAIE
                     ADKKLVQKMTAQGAVFRTAGAAEIKRWRERAAPGNKTFTDKHPDVAQKLAAIEKKCGV
                     GK"
     gene            complement(22907..23404)
                     /locus_tag="VFV55_05900"
     CDS             complement(22907..23404)
                     /locus_tag="VFV55_05900"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX4943867.1"
                     /translation="MAATPARYRASLGRYLEDFTVGDVYEHFPGRTITETDNIQFSLL
                     TMNNHPMHCDHAFAAKSEFGKPLVNSGLTLAIVLGMTVADVSGKAIANLGWTDIKLTA
                     PVHPGDTIYAESEVLDKRESRSRPTQGIVTVRTTGRKADGTVFMSFVRSVLIPKKGHG
                     VQDIA"
     gene            complement(23489..24364)
                     /locus_tag="VFV55_05905"
     CDS             complement(23489..24364)
                     /locus_tag="VFV55_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEX4943868.1"
                     /translation="MKTRLFADLTAFVRAACTHASHLDREVLLLPFYVREHAVGWLRP
                     SFADLLRRWPHVFEVTGSYVTMRATPDTVHGRTAALAEVTQALAKEGVIRGWRGERVS
                     VSHHYAAPEFLQVERAATRHFGMMAYGAHLTGFTRRSGEPCLWIARRAASKSVDPDRL
                     DNLVGGRISSGYTVDETIRKEAWEEAGIPANLLAGLACASVVRVEYSVPEGLHREVIF
                     AHDLWLPEDFRPQNQDGEVARLYCLSIPETIQAILAGEFTLDAGAVTVDALLRNGVLA
                     PEDPQYLDLVRLLRP"
     gene            24414..25667
                     /locus_tag="VFV55_05910"
     CDS             24414..25667
                     /locus_tag="VFV55_05910"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF001933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX4943869.1"
                     /translation="MKVIVLGAGVIGTASAWYLAQAGHDVEVVERRAGPGLETSFANG
                     GQVSVCHAEPWANPGAPLKILKWLMREDAPLLFRLRMDPAQWSWGLAFLHECLAWRTR
                     RNIPQILAMAFHGRDMLQRLRAETGIEYEQSTRGILHFYTDRAEYAAACEAAALMRRY
                     GLDRTVKSVDEAIAIEPALAGAAARIVGATYTPSDESGDAYLFTVNLAKLAEAKGVRF
                     RTGCAIRGLRATGKGVTGVMVSGAAGEEAIVGDAYVVALGAYSPLLTRPLGIDLPVYP
                     AKGYSATMTVADPGKAPRVSLTDDEAKIVISRLGERLRIAGTAEISGWSTDLDPVRCE
                     ALVKRTRSIFPDACDWERPEYWAGLRPSTPSNVPLVGATRIPNLWLNTGHGTLGWTMA
                     CGSGAALAELMSGRRPGIDFEFTRP"
BASE COUNT         3874 a         8716 c         9140 g         4072 t
ORIGIN      
        1 ctgatgctgg tgatggcgga caacttcgtg cagctcttct tcggctggga ggcggtgggc
       61 ctcgtttcct acctgctcat cggcttctgg ttcaagcgcc cgaccgccat ctacgccaac
      121 ctcaaggcct tcctggtgaa ccgcgtgggc gacttcgggt tcctgctcgg catcggcttc
      181 gtgttcgcct acttcggcac gttcggctac gcggaggtct tcgcccgggc gccggacctc
      241 gcgcgaaccg ccatcgagct ggtgccgggg caccccgtgg cgctgggcac cgtcatctgc
      301 atcgggctct tcgtcggcgc catgggcaag agcgcgcagt tcccgctgca cgtgtggctt
      361 ccggactcga tggaaggccc cacgcccatc tccgcgctca tccacgcggc cacgatggtg
      421 acggcgggca tcttcatggt ggcgcgcatg tcgccgctgt tcgagctgtc gcaggcggcg
      481 ctgtcgttcg tgatcgtgat cggcgccatc accggcttct tcatggcgct cgtcgccatc
      541 gtgcaatacg acatcaagcg ggtcgtcgcc tattccacgc tctcgcagct gggctacatg
      601 acgatggccc tgggggccgc ggcctacccg gtggcgatct tccacctgat gacgcacgcc
      661 ttcttcaagg cggtgctctt cctgggcgcg ggctcggtga tcatcgcgct gcaccacgag
      721 caggacatga ggaagatggg cgggctgcgc aagtacatgc cgatcaccta tctgacggtg
      781 ctcgtcggcg cgctggccaa cgcggggctg ccgcccttcg cgggcttctt ctccaaggat
      841 tcgatcatcg aggcgctgca cctgtcgcag acgccggggg cgggattcgc ctgggcactg
      901 gcgatggccg gcgtgttcgt gggcggcttc tattccttcc ggctggtgtt cttcgccttc
      961 cacggcaagg agcgcttcga gctgcatgac gaccatgacc acggcgggca cggagatggc
     1021 catggcgcca ggccgcacga gagtccgtgg gtggtgaccg tgccgctggt gctgctcgcc
     1081 attccgtcgg ccgtcatcgg ctgggtgacg atcgagccca tgctttacgg cggctggttc
     1141 ggcgagtcga tcgccgcctc cgggacgatg aaggagatgg cgcgggagtt ccacggcgcc
     1201 ggcgccatgg tcctgcacgc cttcggcacg ttgcccttct ggctcgcgct cggcggcgcc
     1261 ggtctcgcgg gattcctgta cctggcacgc ccggaccttc ccgcggtcat caaggcccgg
     1321 tcgggcgtgc tcgccacgat cctcgagcgc aagtacggct tcgacgagtt caacgactgg
     1381 ttcttcgccg gcggcgcgcg cggcctgggg cagggcctct tcgcctgggg cgaccgcacg
     1441 gtcatcgacg gggtcatggt caacggcacg gcgaggctca tcggctggtt cgccggcttc
     1501 gcccgcaagg tccagacggg ctacgtctac cactacgcgt tcacgatgat catcggcgtg
     1561 ttcgcgctcc tcaccttctg gtactcgagg atctagaaga agaacatggc gcagggcctt
     1621 ccgatcctct ccgtcgtcac gtggctgccg atcgccttcg gcgtggccgt cctggcgatc
     1681 ggctccgaca ggagccccgc cgcggcgcgc tggacggcgc tggcgggttc cgtgctgggc
     1741 ttcctcgcgg gcgtgccgct ctggacgggc ttcgacgtgg ccgcgggcgg cgcgatgcag
     1801 ttcgaggagc tggccccctg gatcccgctc ttccacgtca actaccacct cggggcggac
     1861 ggcatctccg tgcccctgat cctgctcaac agcttcatga ccgtgctggt cgtcattgcg
     1921 cactgggagg cgatcacgga caaggtgggc cagtacctcg cggccttcct catcatgtcg
     1981 ggactcatca acggcacctt cgccgcgctc gacgcgatcc tcttctacgt gttcttcgag
     2041 gcgatgctca tcccgatgtt cctgatcatc ggcgtgtggg gcggcccgaa ccgggtgtac
     2101 gcggcggtca agttcttcct gtacacgctg ctcggctcgc tgctcatgct cgcctcgttc
     2161 atctacctct acctgcagac gaacggcagc ttcgagatcc tcgatttcca caaggtcccg
     2221 ctgtccatga cggcgcaggt cctcgtgttc ctggccatgt tcatgtcctt cgccgtgaag
     2281 gtgccgatgt ggccggtgca cacgtggctg ccggacgcgc acgtggaagc acccacgggc
     2341 ggctcggtga tcctcgcggc catcacgctc aagatcggcg cctacgggtt cctgcgcttc
     2401 aacctgccca tcgcccccga cgcctcgcac gcgctggcgg gcttcatgat cacgctctcg
     2461 ctcatcgccg tggtctacat cggcctggtg gcgctggtgc agtcggacat gaagaagctc
     2521 atcgcctact cgtcgatctc gcacatgggg ttcgtgacgc tggggttctt catcttcgac
     2581 gccgacggca tgctgggggg cctgatgcag atggtctcgc acggcttcgt ctcggcggcc
     2641 atgttcctgt gcgtgggcgt gctctacgac cgcgtccact cgcgcaacat tgccgactac
     2701 ggcggggtgg ccaacaccat gcccgtcttc gcggccttct tcgtgctgtt cgccatggcc
     2761 aactcggggt tgcccgccac cagcggcttc gtcggcgaat tcctggtgat catgggctcg
     2821 gtgaaggtga acttctggta cgcgttcctc gccggcacga cgctcgtctt cggcgcggcc
     2881 tacaccctgt ggatggtgaa gcgcgtgatc tacggcgagg tggccaacga gcacgtggcc
     2941 gcgttgcgcg acgtgaacgc gcgggaggtc gtgtttctcg tcctgctcgc cgcgtgcgtg
     3001 ctggcgatgg gggtctatcc caagcccttc gccgacgtca tgaacccgac cctgcacgag
     3061 ctgctcaggc acgtgacgac gacgaagcta tgaacaccgc caccttctcg ctcgccacgc
     3121 tggcccccgc gatcccggag atcgcgctgc tggcgctcgt ctcggcgctg ctcgtcatcg
     3181 acctcttcgt ggcggaaaac gagaagcacg tcaccttctg gctcgcgatc ggttcgctcc
     3241 tgggttgcgc cgcgctctcc ggcatgatgg cgggcaacgc ctcggccgtc accttccagg
     3301 gcatgttcgt cgccgacccg atgtcccagg tgctcaaggt ggcggtgctc ctcgcggtgg
     3361 ccgcgacgct cgtctacagc cgcgcctacc tccaggcgcg cgagatgctg cgcggggaat
     3421 tcctgtcgct cgcgctgttc gcggcgctgg ggatgatggt gatgatatcc gcgcaccatt
     3481 tcctcaccct ctacctgggc ctggagctgc tggcgctctc gctctactcg atggtggccc
     3541 tcgagcgcga ctcggcgcgc gcctcggagg cggcgatgaa gtacttcgtg ctgggcgcgc
     3601 tcgcctcggg gatgctcctg tacggcatgt cgatgctcta cggcgccacc gggtcgctcg
     3661 acatcgaccg ggtggccaac gccctcggaa gcgcgcagtc cagccaggcg ctcgccgtct
     3721 tcgggctcgt cttcgtcgtg gcgggcacgg ccttcaagct aggggccgtc cccttccaca
     3781 tgtggatacc cgacgtctac cagggagcgg ccaccccggc cacgctcttc atcggcgccg
     3841 cgcccaagat cgccgccttc gccttcgtga tgcgcctgct cgcgcacggg ctgggcggga
     3901 tggcaggcga ctggcagcag atgctcgcga tcctggccgt cggttcgatc ggactgggca
     3961 acctggtcgc gatcgcgcag acgaacctca agcgcatgct cgcctactcg accatctcgc
     4021 acatgggctt cctgctgctc ggcatcctcg cgggcacgag caacggctac agctccgcca
     4081 tgttctacgt gatcacctac gtgctcatga cgctgggcgc cttcgggatg atcctcatcc
     4141 tgtcgcgcgc cggcttcgag gccgaggagc tcgacgactt ccgtggactc aaccgccgct
     4201 cgaggtggta cgccttcctg atgctggtca tcgtgttctc gctggcgggc atcccgccgg
     4261 ccgtgggctt cctcgccaag ctggccgtgc tgcaggcggc gttcgaggcg ggatacggct
     4321 ggctggtcgt cttcgccgtg ctgatgagcg tggtgggcgc gttctactac ctgcgcctgg
     4381 tgaagctcat gtacatggac gagccggccg gcgaaatcac catcgcgccg cgactcgaca
     4441 tgcgcgtgct cctgtcggcc aatgcgatcg cggtcctggc cctgggaatc gtgccggcgc
     4501 cgctcatgga cctgtgcgcg cgggcaatca ccgccagcct gtaagcctgg tgccttgaag
     4561 ctccctcccg atccgaccgg tgacttcacc gaagccaccg tctccacgaa gctggcctac
     4621 gacggcggct tcctgaagct gcgccgcgac gaggtgcgcc tgccgggcgg cgcctgcgcg
     4681 tggcgggaat acgtggagca tccgggcgcg gtgatgatga tggcgttcct cgacgacgag
     4741 acgatcctcc tggaacgcca gtaccgctac ccgctgcggc gccatttcat cgagctcccc
     4801 gccggaaaga tggaggccgg cgagccgccg ctcgccaccg cccggcgcga gctggtggag
     4861 gaatgcggct acgaggcggc cgactggtgg cacatggtca cgctccatcc cagcgtcggg
     4921 tacgccgacg aggtgatcga gctctacggc gcccgcgacc tcaggttcgt cggcgcgtgc
     4981 ctcgatccgg gcgagcatct cgaggtctta ccggcgcgca tcgaggacgc tctcggctgg
     5041 atcggcgcgg gcctcgtcac cgacaccaag accatgctcg gcctgctgtg gtgggcccgg
     5101 ttcggcagga aggcggcgta gcggcacgcg agcgcattcc ctgcggtgca tcaaatgcag
     5161 gccgggaggc cctgctagac tggcccgata gacccgggag tacggaaaga acaatcatgg
     5221 aaaaatacga cctcctcgtc atcggcggcg gcatcaacgg cgcgggcatc gcaagggacg
     5281 ccgcgggacg cggcctcaag gtcctgctcg tcgagatggc cgacctcgcc tcggccacca
     5341 gcggctggag ctccaagctg atccacggcg ggctgcgcta cctcgagtac tacgagttcc
     5401 gcctggtgcg cgaggcgctc gaggagcgcg aggtgctgct ccacgccgcg ccgcacatcg
     5461 tgcggccgat gcgcttcgtg cttccgcacg accgcagcat gcgcccggtg tggatgatcc
     5521 ggatcggcct gtggatgtac gaccacctgg gcaagcgcgt ctcgctgccg gggtcgcaga
     5581 cggtcgcatt cccgcacgtc gagtacagcg ccggcctgaa atccgagttg cgctccgcca
     5641 tcgtctattc cgactgccgc gtggacgacg cccgcctcac cgtcctcacc gccatgtcgg
     5701 cgcgcgacaa gggcgcaacc atcctcacgc gcacgaagtt cgtggccgcg cggcgcgaga
     5761 acggcgcgtg gcaggccgaa ctgcgcgaca tgcgcagcgg tgccgcgcgc accatcaccg
     5821 ccgcggccgt cgtgaacgcg gcggggccgt gggtgtcgag cgtgctcgac ggcgttccgc
     5881 accgcgcgac cggcgccaag gtgcggctcg tgaagggcag ccacatcgtg gtgcccaagg
     5941 tgcactcgct cggccacgcc tgcctcctgc agaatcccga caagcgcgtg atcttcgtca
     6001 tcccgttcga gggcaagtac agcctcatcg gcaccaccga cgtgccggtg gaaacgcccg
     6061 aggaagcggt cgacatcacc gcgcaggaga cggattacct catcgaggcc gccaaccgct
     6121 tcctcgccaa gcccatctcg aaggccgacg tggtgtggac cttcgccggc gtgcgcccgc
     6181 tctacgacga cggcaagtcc gacccgtcct ccatcacgcg cgactacgtg ctcgagctcg
     6241 acgatgaggg cggcaaggcc ccggtgctct ccctcttcgg cggcaagatc accacctacc
     6301 ggtgcctggc cgagacggcc ctgcaaaagc tctcgccgta cctgccgccc atgggccccc
     6361 tgtggaccaa ggaatcgttc ctgcccggcg gcgacatgcc cgactacaac gccttccgcg
     6421 acgcgatctt cgaccgctac aagggcttcc agcgcgacct gctcgacggc gtcgttcgcc
     6481 gccacgggag ccgcacggcg acggtcctcg gcgacatcca caagcccgag gagctgggca
     6541 cgaacttcgg cgccggcctc accgcccgcg agatcgactg cctcatcgcc gacgagtggg
     6601 ccgagaccgc cgacgacatc ctctggcggc gcacgaagtg cggcctgcac atggacgccg
     6661 cccagcgcgc ggccgtagag tcgtacatcg ccgggcgcaa gccgtagcgg gatagcgcag
     6721 cccgtcgatt cttgctcggt gtaccgtgag aggaagtctc agacgagata tcgatggtgc
     6781 cgcaagtttg cactgtgatg aacggagacg gataggggtc aggttggttg ggtttttttt
     6841 ggttttctgc caggtcgaat tgcatcgggc gtcacggatc aaacctatgt cgaggtgttg
     6901 ccggagtcga tgctggcagc agtttcatct ggcagaaaac caaaaaagaa cccaaccaac
     6961 ctgaccccaa cccctggagt tatcccagcg cagcacgttg atcggacacg aatgccggct
     7021 tccccgatga aggaactcgt cgaattcccc gtcaagaccc gcaaggccgt gcgcggcgtg
     7081 ctgaccgaca tcgacgacac gctcaccacc gacgggcgcc tgcacgcggt cgcctacgcc
     7141 gcgctcgagc gcctgcacga ggcggggctg ctggtgatcc ccgtcaccgg gcgcccggcg
     7201 ggctggtgcg accacatcgc gcgactgtgg cccgtggatg cggtggtagg cgagaacggc
     7261 gccttctggt tccgctacga cgcgaagctg cgcaagctgg tgaagcgcta cgtgatccgg
     7321 gacgtggagc gcctgagccg ggtgcagcgc ctgggcgaga tcgcggtgcg catcctggag
     7381 ggggttccgg gctgcgccat cgcctccgac cagccctacc gcgaggcgga catcgcgatc
     7441 gacttctgcg aggacgtgcg tccgctcggc cgcgatgcgg tggcgcggat cgtggcgata
     7501 atggaatccg aaggcctcac cgccaaggtg agctccatcc acgtgaacgg ctggttcggc
     7561 ggctacgaca agctctcgac cacgcgcctg gcgctcgcgg aggatttcgg cgtcgatgtc
     7621 gagcgccaga agtcgcggtt cgtgttcgtc ggcgattctc ccaacgacca gccgatgttc
     7681 gccttctttc ccaacgccgt cggcgtggcg aacatccgca agatggcgga cctgatgcac
     7741 atcctgccgc gctacgtgac ctcggcgccg ggctcggtcg gcttcgcgca ggttgccgcg
     7801 gcgctgcttg ccgcgcgctg atcagcccgt ctcgtcggga agcttcccga tggcggcgag
     7861 gacggcaagc aagttgtcgc gccggaaagg cttggcgagc gtgccgtcga agccctcggc
     7921 catccgggcc gtgcggaagg cgggcgagtg ctcgacgctg gtcgcgtaga ggcgggtggc
     7981 ctgcggatcg cgcttgatgt ggctgcgcag catggcggcg gcgcctgccc cgtcgagtcc
     8041 ctcgagcgcc gtgtccacga agacgatgtc gtagacgtcc tggtcgagcg aggcgatggc
     8101 ctccgcgagg tcggaggcca ccacgaggct ggagtcgcgc ttcctgagga tgtgcgcggc
     8161 cagcatgtgg ctgtcgcggt gcccatccac gaggaggatc gtcgcgccct tgcgctgctc
     8221 gcgaagcgag tgccgcgtga cgagcgtcgg cgtctcgtcc gagtccaccg aggcgccggt
     8281 gactgcaacg atcgcgtcgt tgagctgctt ggccgccagg ggatagcgca ggtacgcgga
     8341 gatgccgttc tcgcggcacg cgatggcgtc gccgggcttg ccttccgtgg cgagcatcac
     8401 caccagcgtg ttctgcagcg ccggatggtt cttcacgcga aaggcgagca ggaacccgtc
     8461 ctgcggcgcc agccggtccg acgccagcat cagcggaacg gggctgcctt cctcgtgcat
     8521 gcgctcgagg agcgcgatgg ccatcgccgc gttgtcggcc tcgaggggcg tcatgcgcca
     8581 gccgcgcagc aggttcgaca gctcgtggcg ctgcgtcgcg ttggcgctca ccaccagcac
     8641 cgacatgccc accagcgtga cgtaggcggg gcgtcgcggg gtcgtgagcg ggagcaccgg
     8701 gaattcgatc gtgaacgagt agagcggctc cggtggccgc gagcccgcct cgagcttgcc
     8761 gcccatcgcg gccaccatga agcgcgcgac cgccaccccc attcccggat cggccgcggc
     8821 gcgcggcgcg ggcctctcgg acccgcccga cgaggcggtc acggcgaagg agagatggat
     8881 gcccgatgcc gtcgtgtact ccggcgatat cgcaagggcc acctcggcgc cggggtacgc
     8941 ggcgaaggcg ctctcgagga ggttgcgcag cagcagctgc aggcgctcga cgtcgccctc
     9001 gaggtggtcg gggacgtcct gctccacctt gacgcgcagg tggcagcggc actcctcggc
     9061 tgcgtgggcg gttcgcttca cgagatcggc caccgcgggg cgcagccgga agccggcgcg
     9121 ccgcagctcg atgcccccct cgaccttgga gaagtccacg aggtcgccga tcgcgacgag
     9181 cgccgtctcg gtggaggcgc ggatgtcgtg gaattgctgc acgagcgcgg ggtcgaaatc
     9241 catctgttgc gcgcggatgg agatggcgga gagcccttcg agcgccaccg agagctccgc
     9301 gccgatcttc tcgatcagcg cgatctcgga agagccgccg tcggcttccg ccggggattc
     9361 gaccgacgcg gcgcgcgacg accacagggc gcccgccggc tcctcgtcca cgagcaacgg
     9421 ctggcgctcg agcgtcgcgg gcgggttgtc ggccggctgg aacgcaatgc tcgcgggggc
     9481 gtcgggcttc cggtgcgcgc gcgcgagggc ccgggcgtcg acgatcgccg agcggccgat
     9541 gacgtcgatg gcgggcagcc ccaggagcga ctgcggcgcg ctcgcgaggc cgagcaggcg
     9601 gcagaaggcc tcgttcacca gctcgacctc gcccgccgca ttctcgatga gcgccgcgtt
     9661 gggcgaggct tcgaccaggg ccatcagcct cgcggtggtg aggttgagcg cgctctcggt
     9721 ctcgcgctgc ccggcgatgt catgcagcac cgcgacgatt ccggcgggct tgtcgcgcga
     9781 atccagcgcc ggctgcaggg ccagttgcac ccaggcgcca cccgaggcca cgcgcacgtc
     9841 gaggagggcg gatgcggtct ttccctcggc gatgcgcgcg atgttctgcc ggactcgcct
     9901 ggcgtcgtcc ttgtggaaga aggaggcgag cgaggcggcg gacacgccgt gccccgggtc
     9961 gccgcagagt tgcgcgaacg cggggttggc gaaagcgacg acgcccttcg ggtccgcgag
    10021 ggcgaccgcg gtccgcagcg actccacgat gcgcttgaag acctcggtgg gcatggcggt
    10081 ccgccccggg gcggtcagcg ggcctggccg ccctgcgcag ccttgcggat gacgccgaga
    10141 aggtggctcg ttgccgggtg cagcgccgac gccggatccg cgaatgcggg caggacgcgg
    10201 cccgcgatcg ccttgcccag ctcgacgccg aactggtcga aggcgttcac gccccagagc
    10261 accgactggg catagacctt gtgctcgtag agcgcgatca gcgcgcccag cgccgcgggc
    10321 tccaggcaag gcagcaccag ggtcgtgctc ggccggttgc ccgggcagtc gcggtgcggg
    10381 ctgccggtgg agcgcccggt catgagcgct tccgactgcg ccagcgcgtt ggccaggaga
    10441 accctgtgct ccgcctcgcg cgaacccatg gggcgggcga cgacgacgaa gtcgcaggat
    10501 gccgcatcgg tgccctggtg cagccactgg tagaaggcgt gctgcccgag ggtgcccacc
    10561 tcgccgaaga tggcggggca ggtagggcag gtgaccggcg cgccgtgcac gtccacgcgc
    10621 ttgccgttgg attccatttc gagctgctgc aggtagccgg gcaggctcgc gagccgctgc
    10681 gcgtagggca gcaccaggtg cacgggaatg tcgaggaagt cgcggttcca gacgccggcg
    10741 agcgcgagga ggacgggaac gttccgctcc aggggcgcgg accggaattc gaggtccacg
    10801 gcgtgcgcac cggccaggaa gcgctcgaag gcgggcatgc ccagggcgat cgcggccggc
    10861 agccccacgc tcgaccacac cgagtaacga ccgccgaccc agtcgccgaa cggcagcacg
    10921 ttgcactcgg gaagcccgaa gcgggcggct tccgccgcgt tggccgtcgc ggccaccatg
    10981 tgtttcgcga ccgccccctc gcccaggctg gcggtgatcc agtcacgggc cgccgcggcg
    11041 ttggccatcg tctcctgcgt ggcgaacgtc ttcgaggcaa tgatgacgag agtggaggca
    11101 gggtccagcc cggccagcgc gtcgtcgagc gcggcgggat cgatgttggc gaggaagcgc
    11161 acctcggggc cgtcggtccg cgccgcgagc gcctccacga cgagcctcgg tccgaggtag
    11221 gagccgccga tgcccagcga gagcacatgg cggattcgcc cccccttggc gcccttccag
    11281 gtgccgtcgc gcaccgtggt cgaaaggacg cgcatgcgtt ctcggcagcg ttgcacatgg
    11341 gccagcacgt tctcgccgtt ggccgccacc ttctcgtcgc cgcgcagcgc cgtgtgcagc
    11401 gccggccggt cctcggtgac gttgacgtgc tgccccgtga acaggcgctc gatggcgccg
    11461 tgcatgtcac gctcgcgagc cagcgtcacc agccgctcaa gggtctccgg cgtgatccgc
    11521 tgcttggaga aatcgacggc gaggccggcg caggcgaagg tgagggcggg gccgcggccc
    11581 ggatcggcgg caaagagctc gtcgacccgc agcgccgcga tcgcgtcgcg gtgcgcctcg
    11641 agggcttgcc aggcgggggt gggggtcggg gaaggcacgg tcatgcgaaa gtgcggcgcg
    11701 aaggccgcgg atgccttgga ttataactgc cgggggcctg ctaccccacg gtgagaccgg
    11761 ccacgccgag ggccatgccg gccgccgcca ccaggcgacg cgtgaggtcg ccctccttga
    11821 ggaaataggc gcccatgagc gccgcgaaga ggatcgacac ttcgcgcgcc ggggccacga
    11881 ggcttaccgg cgtgaacacc atggcggtga gcacgaggat gtaggacagc ggcgaaagga
    11941 cggcgatcgc gatgacggtc ttgcggcgct cgcgccaggc cttgtcgatc gaacccggcc
    12001 agcgcttctg cgtgaccggc accagcacca gtgaccggaa gaaattgcag ccccagtcgt
    12061 agacgagcgg cggtacgagc caggccgcga cgcttgcctt gtcgcagatc gtgtacatcg
    12121 agatggtgaa gccggtgaga agcgcgaacc ccaccgcgtg ccgggcctcg gacttgtgcc
    12181 aggtgagcgg attgccggtg aggacaagtg cggagatgac gatcaggacg gcgcccgcga
    12241 gcgcggtggg ccccgggcgc tcgccgagta ccgcgaccgc cacgacgatg gtgagcagcg
    12301 gcccggtggc ccgggccagc gggtagacga tggacaggtc gccgccgctg cgatacgcgc
    12361 ggtcgagcag caggaaatac gccgtgtgga tcatcccgct ggtgaacatg agagccaggt
    12421 gcacgggcct gaagccgtag tgctgcaccc acgccgcgcc ggcaacgaac ggcgcgtaca
    12481 cgaccacgga gccgaggcac gcggcggtga tgagtccggg gccgccgccg ctcttcttca
    12541 gcaggaagtt ccagctcgcg tggatgagcg cggcggtgag gacaagcgca agggcgagcc
    12601 aggtcatcga attgcctgtg gtgtcagaca cggtgtcaga caccgtgtct gacaccgcat
    12661 gtttacactg gccggcgcat caggttcacg cgcatgggcc gcacgaagcg ggcgccgccg
    12721 ggccccatgt gcccttcgaa gcggcggcgc acttcggcca gccgctcgcc cacgagcgtg
    12781 tggtccgagt gggtcacgcg cacgatgcgg tcgaagaagt cgtcgaaatc gcggaaggcg
    12841 agggggacgt cgaactgccg ctcctccacc tgctccagcg cgcccgaggc ggcggcgcgg
    12901 cgaagcgcgg ccagcgccgc agcgcgcacc gcctcctcgt cgtggaagag gcgcacgatc
    12961 tcgttgaacg ggccggcgaa gacgggctcg gagacgtaga gcagcccgcc cggcacgagc
    13021 acgcgccgga tctcggcaag cgccgcgttg agccgctcga cgggcacgtg atgcagcgac
    13081 ttgaacatca gcgcgatgtc gaagttcgca tcgcggaacg gaatggcgtc ggcgcccgcg
    13141 aggaagaact cgagtcccgc cggccgcggc cctgcgaggt tcttcgcgtg ctggacctgg
    13201 tcgacctcca gggcggatac ggaggcgacc aggccgccct cgacgagtcg gcgcgccatg
    13261 tccgccgctc cgcagccgag atcggcgacc cgcgcaccgg ccagcggcag caaccgccgc
    13321 atcaggtcca gctcgtcctt cacgagctcc acggtgttcg cgctcatgcc gccgcctgcg
    13381 cgagcagcgc gcgcgcccgg tcgatgatcg gcaggtcgat gagctttccg tccaggcgca
    13441 cggcgttgtt gccgccggcc tcggcggccg cgagcacgcg ccgggcccac gcgagttcgg
    13501 cctccggcgg gacaaacccc gcattgacgc ccgccacctg cttcgggtgg atgcagagct
    13561 tgccgccgaa gcccaggcgc cgcgcgcgtt ccacgtcctc gcgaagcagg gcctcgtcgt
    13621 cgatcgcgac cgtcacgccg tccaccggcg cgagcctgcc ggcgacgcgc gaggcgagca
    13681 ccaggtgcga gcgcgcgaaa agcagttcct cgcgatcccc ggcgattccg gtgtcgagct
    13741 ggaagtcgac ggacccgaag gcgaggcgct cgacccccgg cgcggacgcg agcgcctcgg
    13801 cccgccacag gcccagcgcc gattcgatga gcgcaatgat gcgcacgccg cttccggcgc
    13861 gtgcgcggat ctcgccgatg gcggccgcgt cctcggcctt gggcagcatc acgccccgga
    13921 tccccggcaa tcccacgagg tcgaggtcgg ccgcgaacca ttcgctctcg gggccgttga
    13981 tgcgcagcca gacgggcttc gccgccgaga gccatgcgcg cgccgactcg cgggcacccg
    14041 ccttgtcggc gggcgccacc gcatcctcga ggtccacgat caccgcgtgc gcaccggcgc
    14101 cgtacgcctt gtcgaaccgc tccggccggt tgccgggcac gaacaggaaa gaccggtcca
    14161 tcatccgttc tccaggttgc cgggcgtctc gcgcccgggc tcattgcgaa agccgtcctg
    14221 ccacaacagc cacaacccgc aggccacggc cggcaccgcc acgatcatga acgccgtcga
    14281 atagcgggtt ccgctttcga cgaggaacgc gaaggcgggc ggcccgaaca gcacgccgcc
    14341 gtaggtgaag acgagcgagc cgccggtggc gtcgcccgcc tttcccggcg gcgcgcggcg
    14401 cgcgatctgg gcgagataga ccccgttcca gccgatggcg gtggcgccga aagccgcgca
    14461 gacgaaggcg acggcagccg ctggccaggc ggccgtgaac tgcgcggtcg cgatggcggc
    14521 gagccccatg gcgatcgcga ccagtccgag gagccgcatg ggacggcccc agcggtcggc
    14581 gagcgcgccc cacgcgatgc gggcgaccac gccgccgccc tgcgcgacag tgagcatgag
    14641 gccggcctcg acgagggaaa agccgaggtt ggccgtgagg tgggtgacga ggtaggtgat
    14701 gaggcaaagc tgcagggagg cgaagaagaa cgagcagatg gcgaggcgcc gcagctccgg
    14761 attgccgagc gtgtagcgca gcggtccggc gagacccgag accgacaggc gccgggtggg
    14821 gtcgcggtcc gcgtcgagcg aggcgcgcag cggttgggcg aggagcaggg tcgcaaggca
    14881 tgccgcgcct accgcgagag cggccgcgcg ccagccggcc accagcacca gggcaggcac
    14941 gacggcgccg gccagcgcgc cgcccagcgg cacgccggtc tgcttgagcg agaagacgag
    15001 ggacatcatg ccgggcggcg tggtccgggc gagcacgtgg gagctggccg gcgtaaccgg
    15061 cccgtagccg aagcccagca ggatcgcggc cgccacgagg aaggcggcgc caccggttgc
    15121 ggtgagcgcg agtccggcgg cgcacgagac gaggcaaagc tggctcacgc ggatggcgcc
    15181 gaagcgttgc acgagcgtgc ccgaaaccag gctcgcggcc atgctggcgc cgtagacgag
    15241 cgcgatgaag aagcccacgt agccggccga tacgcccagc tctgcggcgg cggcaggcgc
    15301 cagcaccggc acggtcacca ccgccatcga cacgaggagc tggaccgcga gcgtggcggc
    15361 cagcgggaac agcgtgctac gcatccgccg cggccgggac ctgcaggcgc atcgacatcg
    15421 agtacgagaa ccggtccgcg gggtggaggt tgacggccac ctcgatgggg cggtcgttgt
    15481 cgccgaagta gcggcgcgtg acgacaaggc ccgccgagcc cggcttcacg ctcaggcgct
    15541 tcgcgtccgc ggcgccgatg gtggcggcgc ggatctcctg cttcacctcg acgatggtga
    15601 tgccgtactg cttctcgatg agcgtgtaca cgggcacctt cagggtgccg atgagtttcc
    15661 ggaccccccc gtaggctgca ttgatgtaga tctcggtgag cgccatgggg agcttctcct
    15721 tgcccgcgta gcggaagccc gtgaccttaa cccagcgctg gccggccggg cagcgcagga
    15781 gctcggcgag gaactcgtcg gcgatcacgt ccgccgcggc ggtggtcacg agccgcgtgt
    15841 ccgcgacgta ttgcggaagg tccgagatcg aggcggtgga ctggacgtag cggctgtcga
    15901 cgcgggtcgc cttcacgctc gtgccgatgc cgcgctggcg cgtgaccagg ccgtgctcgt
    15961 gcaggcggcg gatcgcctcg cggacggtgt gccggcttac gccgaactgc gtgcacagct
    16021 cgaactcggt gggcagcagg ctgcccaccg ggtaacggcc gccggtgatg tcgtcgagaa
    16081 ggcgctgggc caccagcgcg tactggggct gcctcgcgac cagcggaaag ggggaacgcg
    16141 cgctcacagg gctccggctt gcgacaggtg atcgatgcgg gcgtcatcgt agccgagcgc
    16201 gcccaggatc tcgcgggtgt gctctcccag cgtcggcgcg atcccgacct tctccgcctc
    16261 gccgggacag cggatggggc aggcgatcgt gcggatggtg ccgcgcgcgg ggtggggcgc
    16321 ttccatgatg cggccctgct ccttcacgaa cgggttctcg agcgcctggg ccacgtcgta
    16381 gaccggcgcc accggaaccc ttccggccag ccgcccgatc cactcggcgg tggtggcggt
    16441 cgagagcacc tcgtccatgt cgcgggtgag gcggtcgcga ttggcgaggc gcgacttgta
    16501 gttgccgtag tcggcaccgg tcgcccattc cggcttgccc acggcctcgg caaagacgcc
    16561 ccagaacttc tccttgttgc acatcacgaa gatccagccg tccttcgtcc ggtagagctg
    16621 gctcggcgtg agcgacgggt gggcgccgcg cggctcgcgg ccgatcatcg cgccttcgtt
    16681 caggtaccac gccgccgggt agttgaggtt ggtgagcgcc acgtcgaaga ggctcacgtc
    16741 gatgtcgcgc ccgcggcccg tggcgcgcgc ctcgatgatc ccggcgagca atcccaggac
    16801 ggcggtgacg ccggtcatca ggtcgacgat ggacagcccg aagcgcgccg ggggcccgtc
    16861 gggctcgccg gtcaccgaca ggtagccggc ttccgcctgc atgaggtagt cgtatcccgg
    16921 ccagctcgcg cgcgagccgc ttcgtccgta ggccgacagg tgcccgcaga cgatgcgcgg
    16981 attgtgcacg gacaggtctg cgtaggtgag tcgcagcttc cccgcgaggt cgccgcgcag
    17041 gttgtcgaag gtggcgtcgg cgctcttcac gaggtcggcg agcacgccgc gggcctcggg
    17101 gttcttgagg ttcagcgtga ggctcttctt gttgcggttg aaggcctcgt agaagtggct
    17161 gtcgccggga ccgaagtagt acgggcccac gaggcgcccg acgtcgccgc cctcggccgg
    17221 gttctcgatc ttgatcacct cggcgccgag gtcggccagg tgctgcgtgc cgaacggccc
    17281 cgcgccgtac tgctcgacgg cgacgatcct cacgccctgc agcggcaggc tcatgcgggg
    17341 tttcgctcga tgagctgctt ggcgatgatg atgcgctgca tctcgttggt gccctcgccg
    17401 atgagcagca ggggagcatc gcggaagtag cgctcgacgt tgtactcctt cgagtagccg
    17461 tagccgccgt ggatgcgcat gcactccagc gcgttttcca tggcggactc ggtggcgaaa
    17521 tacttggcca tgccggcttc catgtcgcag cgctcgccgc ggtcgtaggc acgggcggcc
    17581 gactccacca gcaggcgcga ggcttccacg cgcgtggcca tctcgccgag cttcagctgg
    17641 atggcctggt gctcgcagat gggcttgccg aaggtcttcc tcgtctggga gtaggcgacg
    17701 gcttccttga gggcctccct cgccacgccc acgccgcgcg cggccacgtt gatccggccg
    17761 agctccaggc cgctcagtac ctgctgcagc cccttgccct cgacgccgcc gatcaggcgg
    17821 tcggccggga tgcggtagtt ctcgaacacc agctcgcagg tgtcgatgcc gcggtagccc
    17881 agcttctcga gcttcctcgc caccttgaac cccgggccct tctcggcgat gaagaggctc
    17941 atgcccttgt gccgcggctt ggcctgcggg tcggtcttca cgaggagggc gatggtgttg
    18001 ccgtacatgc tgttggtgat ccacgtcttg gcgccgttca ccacgtagtc gtcgccgtca
    18061 cgcacggcca ccgtgcggat cgcctgcagg tcggtgcccg cgtcgggctc ggtgaggccg
    18121 atgcctccgc gcagctcgcc ggtggcgaat ttcggcaggt agtggcgctt ctgcgcttcc
    18181 gtgccgctgc gctggaccgc ggccgccatg atgaggtgcg agttgatgat cccggagaca
    18241 ctcatccaca ccgccgccat gcgctcgatg atcttcgcgt aggtggaggt gctcaggccc
    18301 aggccgccgt actccgggtc gatgatgcag ccgaagaggc ccagctcctt catcttgtcc
    18361 acgatctcct gcgggtactc gtcggccttc tcgaggcggt gcgcgttcgg gcgcacctcg
    18421 gcctcgagga acttgtcgac gctgtcgagg accatctgtt ccatttcgag ttgctcggga
    18481 gtcacggcgg tttcctttcc ggtgtccggg gtgtcgggtc gtcagccgcg cgcggcgagg
    18541 ccctcggcga ggtcgcgcgc atcgaggccg gggccgatgg cgagcaccag gtcgcggatc
    18601 tcctcggcgc gggaagcgga caccgcgagg cgcgcgttgg ccatgaactt cctctcgatc
    18661 tcgccgttgg tgatggggcg gtcgacgcag ccgcggttca tctcctcgcg atggcgcagt
    18721 tcgcgcccgt cgttcgtggt gacgatcacc tcgcccgagt agtacttcgg gaagggggag
    18781 gcgggatcgg cttcgtattc gacgcgctgc gcgagtgcca gcacctcggc gtcggcaagc
    18841 gcggcgtcct cgaggtgcgc cagtccgaag gacccgcggg cgagcgcggt ggcgacgatg
    18901 tagggaatgc tgaactgggc gtcgtagctg ttctgcgggc gcttcttcgt ggccaccggc
    18961 tcgcacaccg tcttcaccac ttccttcggc acgagggcgc gcaccgagcg gatgtcggac
    19021 accttgaggc cgtgcttgtc gcgcaggatg gcggccgcgt cggcgcaggc gtgcgtgaag
    19081 tggcatgcgg gaatgggctt caccgcgacc ctggccactt cccacgtctc gcccagcccg
    19141 gcggtggcga gcgagagatc gatatccttc gcgtactgcc cgaggtggct cgcgaagagg
    19201 ccgaaccgcc cctcgtaggc ctcgcgcggg ccgacgaagc cgtggcgcgc gagggaggcg
    19261 gcggtaaagc cggccacgcc cgcccagccc gggtgcatgc gcttggtcca cgcgccgtcg
    19321 ttcaggaatt cgagactgcc ggaagccgcg ctgagcgcga ttccctgcgc catcgcgagc
    19381 tgcgccgcgt ttagcccgaa gaggcgcccg gcgatgagcg tggcggaaaa cgcgccgacc
    19441 agccccgttg ggtggaagcc gatctggtgg aaggcgcctt tcgcgaccga acccaggcgc
    19501 gtgcccgcct ccatgcccac gacgtaggcg gcgaggaggt cctggccgtc gaggccggcg
    19561 tgggccgcga cgcccagggc gctcgggaag cacgcggccg tcgagtggat gacgccctgc
    19621 acgtgggtat cgtcgaagtc gaggccgtgc acgaggatgc cgttcatcag catcgcgtcg
    19681 cgcaactgca gccgcgcggg caggccgatg accggcatgt cgcccagccc ggcggagagg
    19741 tcggtcatgg cggatagcga gcggtgcgcg aactcgtagc gcgtggacgc gtgcgcgatg
    19801 ccgaccgcat cgaggatgag gtaccgggcg cgctcgagga ccgcggcggg aatcgcctcg
    19861 ggccgcaggc tctcggcgaa ggcggcgagc gtttcggcga tggtggcgcc gcgcgccgcc
    19921 gcttcgggcg ccgtgccgac ggggatggcc atgttcgtca tgggaatgtc ctttcagtct
    19981 cgttacagga gcacgaggga gatggcgggg atgtaggtga tgagcatgag gcccgtgagg
    20041 cgcacgaaat agaacggcag cacgccgcgc accacctcgt gcaccgggag cttcgcgatg
    20101 ccgctcatca cgaagaggtt gagcccgatg ggcggggtga gggtggcgat ctccacgttg
    20161 gccaccatca ccacggcgaa gtggatgggg ttgatgccga gcgcggtggc cacgggaaag
    20221 agcagcggcg aggtgagcac gatcatcgcc acgccatcca ggaacatgcc gagcaccagc
    20281 aggaccaggt tcgcgaccag caggaactgc caggcgctca tgttgatgct ggtgatgtac
    20341 tgcacgacgc tggccgagat tcccatgcgc gcgagcacga agcccaccag gcttccgccc
    20401 atcaccaccg cgaagatcat ggtggtctgc agcaccgagt cgcgcagcac ggtccacacg
    20461 ccgcccacgg tgacgctgcg gtagacgagg atgcacagga tcgccgcgta gacggcggcg
    20521 aaggccgaga cctcggtcgg ggtgaggtag ccggtataga tggagccgat gacgagcacc
    20581 ggcatgagga gggcggcgcc cgccttcggc agccgcctgg cgaacccgcc ccagtccgcg
    20641 cgggcggtcg tggtgccgta gccgtagtgc cgtgacatcc acgcgacggc ggtgaggagg
    20701 agggtggcct ccatgaggcc cggcatcacc ccggcgatga agagccggtc caccggcagc
    20761 ccgacgatcg agccgatgag gatgaagccg agcgagggcg ggatcatgag gcccagcgtt
    20821 ccgcccaccg caacgatgcc ggccgagaag cgcgccgggt aatcctcgcg cttcaggccc
    20881 tcgacggtcg ccgcgccgat ggccgcggcc gagcccacgc tggagcccga aatggccgcg
    20941 aagaagacgc aggcgatcat cacgctgatg gcgagcccgc cctgcacggc gcgcacgatg
    21001 ctgccgatca ggtcgatcat caggccggcg atgttgccgc gcatcatgag gttgcccgcc
    21061 aggatgaaca tcggcaccgc catgagcggg tagctgttca ccgccttgaa ggcggtctgc
    21121 gcgatgacgg tcatcggcac gccgtcgatc atgaggatga ggccggtggt gaccaggccc
    21181 agcgacacgg ccacgtgcga gccgatggcc aggagcgcca gcatcaggac gatggcgagg
    21241 atcatcagca tggaagttcc ccggccctag agctcggagc ccgtcgggcc ggccgagacg
    21301 aacgggtcgc cgcgccgcag gagcaccacg aagcagcgca tgtagtagcc cccgaggagc
    21361 gccgcgccga tcggcatggc gaggaagacg atccactgcg ggatgtcgag attcgactcc
    21421 gacaccatgc ccatcgtgta gtagcggatg agctgcggga tcgaggaggc gaaaaggacg
    21481 gcgctgaacg cgatgccggc cacgacggag gcgaagtgca tggcgcgccg gatccccggc
    21541 ggcatcgcgt ccaccagcat ctcggtgcgg atgtggtgcc cgtgccgccc cgccacgccg
    21601 agcgtgagga agaacgccca gatcatgagg tagcgcaccg attcctcggc ccagaagaag
    21661 ctcacgtcgc ggaaggaccg gttgagcccc tccgagagca tcaccagggt ggaggcgagg
    21721 aagagcagcg cggagaagtt gagcaccggc ccggactcga accagtccca ccaaccgcgc
    21781 agcggcgcgg ttgcacgatc gggcttgctc accttacttg cccacgccgc acttcttctc
    21841 gatcgccgcg agcttctgcg cgacgtcggg gtgcttgtcg gtgaaggtct tgttgcccgg
    21901 cgcggcgcgc tcgcgccagc gcttgatctc cgccgccccg gcggtgcgga agacggcgcc
    21961 ctgggcggtc atcttctgga cgagcttctt gtcggcctcg atggcctccg cccactggcg
    22021 caggccgatc tccgcgatcg tgtcggtgac ggccttcttc tgtaccgcgg gcaggccgtc
    22081 cagccaggcc ttgtccacga ccacggcgta ggtggccacc gacatgccgg gcacgtacca
    22141 cgcgtacttg gcggttatcc cgatcatctc ggcccagccg gcgggcgagg tgaagacgcc
    22201 gtcgatcgcg ccctgcgaaa gcgcggtgct catctccgag gcagccatgg agacggggct
    22261 ggtgttgagc gagcgcatgg tgtcgaggaa gaccttgccg ccggtcacgc gcaccttggc
    22321 gcccttgagg tcctcgagct tctgcacgtc gcggtccttg aagacgaaca gaaggtcgga
    22381 ggtgcgcagg aagccgagca cctggagctt gcgcggctcc acgtaggacg acatgagctt
    22441 ggtgaggtcc tcggcaaagc agcggttggt catcagctcg tcggacagga cgaagggcag
    22501 gttgatgatg ccggtgcgcg ggtcgatggt ctccaggcgc accgcgaccg gccacaccat
    22561 gtgcacggca ccggtgccca gggccgccat cgcgtcctgg tcgttgtaga gctggccggc
    22621 ggggaacacc ttcacgacga gggcgccgcc ggtgcgcttc ttcacctctt ccgcgaactg
    22681 gttcatgtag ccggtcttcc agtgcgtggt ggcgacctcg ctgggcagga ccagttcggc
    22741 ggcccgcagg gcgggcgctg cggcgagtgc gatggctgcg gcgatggcgg cggtggccgc
    22801 ccggaaagcg ggacctcggg tcatgggtgt ttcctccagt tctgtcggtg ggtgctgcga
    22861 tgacgcggcg cgggtctgtc gccctctcgt ccgcgggcgg gtccggtcag gcgatgtcct
    22921 gcacgccgtg gcccttcttc gggatgagca cggagcgcac gaagctcatg aacaccgtgc
    22981 cgtccgcctt cctgccggtc gtgcggaccg tgacgatgcc ctgcgtgggc cgcgacctcg
    23041 actcgcgctt gtcgagcacc tcggattccg cgtagatggt gtcgccgggg tggacgggcg
    23101 cggtgagctt gatgtccgtc cagccgaggt tggcgatggc cttgccgctc acgtcggcca
    23161 ccgtcatgcc gagcacgatg gcgagcgtga ggccgctgtt caccagcggc ttgccgaact
    23221 cgctcttcgc ggcgaaggcg tggtcgcagt gcatcgggtg gttgttcatg gtgagcaggg
    23281 agaactggat gttgtccgtc tccgtgatcg tcctgccggg gaagtgctcg tagacgtcgc
    23341 cgacggtgaa gtcctcgagg tagcgcccga ggctcgcgcg gtagcgggcg ggggttgcgg
    23401 ccatgctttt ctccggggat gatttacgga catccaaatg tccggacatg gtgctccccc
    23461 cgtcgcggcc gtgtcaaccc ccgcggggtc agggccggag caggcgcacg aggtcgaggt
    23521 actgcgggtc ttcgggggcg agcacgccgt tgcgcagcaa cgcatccacg gtgacggcgc
    23581 ccgcgtcgag cgtgaattcg ccggcgagga tcgcctggat ggtctccggg atgctcaggc
    23641 agtacaggcg cgccacctcg ccgtcctggt tctgcgggcg gaaatcctcc ggcagccaca
    23701 ggtcgtgggc gaagatgacc tcgcggtgca ggccctcggg caccgagtac tcgacgcgca
    23761 cgacggaggc gcacgccagg ccggcgagga gattggcggg aatgcctgct tcctcccacg
    23821 cctccttgcg gatcgtctcg tccaccgtgt agccgctcga gatgcgcccg cccacgaggt
    23881 tgtcgaggcg gtcgggatcc acgctcttgc tggccgcgcg ccgcgcgatc cacaggcagg
    23941 gctcgccgct gcgccgcgtg aaaccggtga ggtgcgcgcc gtaggccatc atgccgaagt
    24001 ggcgcgtggc ggcacgctcg acctgcagga actccggcgc cgcgtaatgg tgcgagacgc
    24061 tcaccctctc gccacgccag ccgcggatca cgccctcctt cgcgagggcc tgcgtcactt
    24121 cggccagcgc ggcggtgcgg ccgtggacgg tgtccggggt cgcgcgcatc gtgacgtagg
    24181 acccggtgac ctcgaagacg tgcggccagc ggcgcagcag gtccgcgaac gacggccgca
    24241 gccagccgac cgcgtgctcg cgaacataga agggcagcag cagcacctcg cggtccagat
    24301 gcgaggcatg cgtgcacgcg gcccggacga aggcggtaag atccgcgaat aggcgcgtct
    24361 tcattcgtcg attagtctag gccatttccc ctcattccgc gccgacaccc tccatgaaag
    24421 tgatcgtcct tggcgccggc gtgatcggca ccgccagcgc ctggtatctc gcgcaggcag
    24481 gccacgatgt ggaggtcgtc gagcgccgcg cgggcccggg cctcgagact tcgttcgcca
    24541 acggcggcca ggtctcggtg tgccatgccg agccgtgggc caacccgggc gcgccgctga
    24601 agatcctcaa gtggctgatg cgcgaggacg cgccgctgct cttccgcctg cgcatggacc
    24661 cggcgcagtg gtcgtggggc ctcgcgttcc tgcacgagtg cctggcgtgg cgcacgcgcc
    24721 gcaacatccc gcagatcctc gcgatggcct tccacggccg cgacatgctg cagcgcctgc
    24781 gcgccgaaac cggcatcgaa tacgagcagt cgacgcgcgg catcctgcat ttctacaccg
    24841 accgggccga atatgcggcc gcctgcgaag ccgccgcgtt gatgcgccgt tacggcctgg
    24901 accgcacggt gaagtcggtc gacgaggcga tcgccatcga gcccgcgctc gccggcgccg
    24961 ccgcgcgcat cgtgggcgcc acctacacgc cgtccgacga gtcgggggac gcctacctgt
    25021 tcaccgtcaa cctcgcgaag ctggccgagg cgaagggcgt gcgctttcgc acgggctgcg
    25081 cgatccgcgg gttgcgcgcc acgggcaagg gtgtcaccgg cgtgatggtt tcgggtgcgg
    25141 cgggcgaaga ggcaatcgtc ggcgatgcct acgtggtggc gctgggcgcc tacagcccgc
    25201 tcctcacgcg cccgctgggc atcgatcttc ccgtctatcc cgccaagggc tactcggcga
    25261 cgatgacggt ggccgatccc ggcaaggcgc cgcgcgtgag cctcaccgac gacgaggcga
    25321 agatcgtgat ctcgcgcctg ggcgagcggc tgcgcatcgc gggaaccgcc gagatctccg
    25381 gctggtccac ggacctcgac ccggtgcgct gcgaggcgct ggtgaagcgc acccggtcga
    25441 tcttccccga tgcctgcgac tgggagcggc cggaatactg ggccggactg cgcccgtcca
    25501 cgccgtcgaa cgtgcccctc gtcggcgcga cgcgcattcc caacctctgg ctcaacaccg
    25561 gccacggcac gctgggctgg accatggcct gcggctcggg cgccgcgctg gccgagctca
    25621 tgagcggccg tcgccccggg atcgacttcg agttcacccg gccgtgatgc gaggaggcag
    25681 cgcggttggg ccgtcgcaat gggcattccc ctcaatttgg agttcgggcc gtcctaattg
    25741 gcattcccct caatttggag gtcgggccgt cgcgcaccac cgggaccagg tgtggttggg
    25801 tt
//
LOCUS       DASUEV010000181         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_732010, whole genome shotgun sequence.
ACCESSION   DASUEV010000181 DASUEV010000000
VERSION     DASUEV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732010"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFV55_05915"
     CDS             complement(<1..405)
                     /locus_tag="VFV55_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4943870.1"
                     /translation="MSATAGASARPIVQITHLAKGYRRGGQDVPVLFDITLSIPEGDF
                     IALMGPSGSGKSTLLNLIAGIDKPDAGELLVGGEDITKLSEAELADWRASHVGFVFQF
                     YNLMPVLNAFENVELPLLLTSLSRRERRGRVEM"
     gene            complement(402..1619)
                     /locus_tag="VFV55_05920"
     CDS             complement(402..1619)
                     /locus_tag="VFV55_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4943871.1"
                     /translation="MSAQDLSRLQIDRGAKSFGPRRRPRWLKFAIVAAIVAAIAAAVA
                     LRSAMAPVTVDVATVTTAFPSQGFTVLNATGRVVAWRKAAISTKATGRLEWLGVQEGT
                     RVKAGQVIARLESLDVAATRDSTVAGLNAARANLEQGEAEMRDADSAYKRAQDLFEKK
                     FISESTLDTARARAEKARASVSSLKAAIGVANANVRSASVSVEQTLIRAPFDGIVLTK
                     NANVGDIITPFSSAADSKGAVVNMADMETLEVEADVSESSISKIAVGMPTEIQLDAYP
                     ELRLLGEVSRIVPTVDRSKATLLVKVAFKDKDPRALPDMSAKVGFLQRAPDASERKAV
                     IAVRPEAIAMRGGKPVVFVLDKDNKVKQTPVTVARKLGDLLEIAGASPGDKVALSPPE
                     KLKDGATVALLKK"
     gene            1732..2055
                     /locus_tag="VFV55_05925"
     CDS             1732..2055
                     /locus_tag="VFV55_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX4943872.1"
                     /translation="MSTQMLNWSAIDADPRFKELHRRKTLFLWGLMAFSVVYYFALPI
                     GAAYATDIYKIKVWGVINVGLLFAFSEFIVAWGIAFFYAREAAKFDRMAEEINRDAHT
                     IGAKP"
     gene            2052..>3214
                     /locus_tag="VFV55_05930"
     CDS             2052..>3214
                     /locus_tag="VFV55_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX4943873.1"
                     /translation="MKIARVSRWAVAAFALGAPAVAFAQTAVNPKYIWLTFAVFAAII
                     SLTMYITYVAAKRTKTAADFYAAGGGMSGLQNGWAIAGDYLSAASFLGIAGLISLNGY
                     DGFMYSVGWLVAYITVLLVIAEPCRNIGKYTMADILAFRNDPKTVRTFMAISTITVST
                     FYLTAQMVGGGVLVKTLIGIDYEYSVMTVGVLMLAYVVFGGMVATSYVQIVKAVLLVI
                     ASLLMVIMVWSQYGFSLPGFLQAAVNDPNIQGRVATMLGDSARNMTPAELGQRFLEPG
                     LLFKNPIDQISLGMALVLGTAGLPHILMRFFTVPTAQAARKSVIWAMAIIGGFYVLTL
                     FLGLGAAMKVGPSGITLIDKGGNMAAPLLAQYLGGGADSMLGNLMLAFVAAVA"
BASE COUNT          488 a         1086 c         1075 g          565 t
ORIGIN      
        1 catctcgacg cgcccgcgcc gctcgcgccg cgatagcgag gtgaggagca gcggcagctc
       61 cacgttctcg aacgcgttca gcaccggcat gaggttgtag aactggaaga cgaaaccgac
      121 gtgcgaggcc cgccaatccg cgagctcggc ttcggagagc ttcgtgatgt cctcgccgcc
      181 cacgagcagc tcgccggcgt cgggcttgtc gatgccggcg atgaggttga ggagcgtgga
      241 cttccccgag cccgacggcc ccatgagcgc gatgaagtcg ccttcgggta tcgagagcgt
      301 gatgtcgaag agcaccggca cgtcctggcc gccgcgccgg tagcccttgg cgaggtgcgt
      361 gatctgcacg atggggcgcg cgctcgcccc ggcggtcgcg ctcacttctt gaggagcgcg
      421 acggtcgcgc cgtccttgag cttctccggc ggcgacaatg ccaccttgtc cccggggctg
      481 gcgccggcga tctcgagcag gtcgccgagc ttgcgcgcga cggtgaccgg cgtctgcttc
      541 accttgttgt ccttgtcgag gacgaagacg accggctttc cgccgcgcat ggcgatcgcc
      601 tccgggcgca ccgcgatcac cgccttgcgc tcgctcgcgt ccggtgcacg ctgcaggaag
      661 ccgaccttgg cgctcatgtc cggcagcgcg cgcggatcct tgtccttgaa cgccaccttc
      721 accaggaggg tcgccttgct gcggtccacc gtgggcacga tgcgcgacac ctcgcccagc
      781 aggcgcagct cgggataggc gtcgagctgg atctccgtgg gcatgcccac cgcgatcttc
      841 gagatggagg attccgacac gtcggcctcg acttccagcg tttccatgtc ggccatgttc
      901 accaccgcgc ccttggaatc ggcggccgag gagaacggcg tgatgatgtc gcccacgttg
      961 gcgttcttgg tgagcacgat cccgtcgaag ggcgcgcgga tgagggtctg ctccaccgac
     1021 acgctggccg agcgcacgtt ggcgttcgcc acgccgatgg cggccttgag gctcgagacc
     1081 gaggcgcgcg ccttctccgc gcgggccctg gccgtgtcga gggtggattc ggagatgaac
     1141 ttcttctcga agagatcctg cgcgcgcttg taggccgaat cggcgtcgcg catctccgcc
     1201 tcgccctgct cgaggttggc cctggcggcg ttgaggccgg cgacggtgga atcgcgcgtg
     1261 gcggccacgt cgaggctttc cagccgcgcg atcacctgcc ccgccttcac gcgcgtgcct
     1321 tcctgcacgc ccagccactc gaggcggccc gtcgccttgg tggagatcgc ggccttgcgc
     1381 caggcgacca cgcggccggt ggcattgagc acggtgaagc cctgcgaggg gaaggcggtc
     1441 gtgaccgtgg cgacatcgac ggtgaccggc gccattgcgg agcgcaacgc gacggccgcg
     1501 gcgatggcgg ccacgatggc cgcgacgatc gcgaacttca gccagcgcgg ccgccgcctg
     1561 gggccgaagg actttgcgcc acggtcgatc tggagccggg ataggtcctg ggcggacacg
     1621 ctgtaccttg ggggaaagaa ggcgcaaatt atacgcgcgc cgactggggg tcctcgatcg
     1681 ctgcacgccc gattccggga aaggcgccca accaaaaagg agaacttgca catgtccacg
     1741 caaatgctca attggtcggc gatcgacgcc gacccccgct tcaaggagct gcaccgccgc
     1801 aagaccctgt tcctgtgggg cctcatggcg ttctcggtcg tgtactactt cgcgctgccc
     1861 atcggggcgg cgtacgccac cgacatctac aagatcaagg tctggggcgt catcaacgtg
     1921 gggctgctgt tcgcgttctc cgagttcatc gtcgcctggg gcatcgcgtt cttctacgcg
     1981 cgcgaggcgg cgaagttcga ccggatggcc gaggaaatca accgcgacgc gcacacgata
     2041 ggagccaagc catgaagatc gcccgagtgt cccggtgggc cgtggcggcc ttcgcgcttg
     2101 gcgcgcccgc ggtcgccttc gcccagaccg cggtgaaccc gaagtacatc tggctcactt
     2161 tcgccgtctt cgcggcgatc atcagcctga cgatgtacat cacctacgtc gccgccaaga
     2221 ggaccaagac cgcggcggat ttctacgccg ccggcggcgg catgagcggc ctgcagaacg
     2281 gctgggccat cgccggggac tatctctcgg cggcgtcctt cctcggcatc gccggcctca
     2341 tctcgctcaa cggctatgac gggttcatgt actcggtggg ctggctggtc gcctacatca
     2401 ccgtgctgct ggtgatcgcg gaaccctgcc gcaacatcgg caagtacacg atggccgaca
     2461 tcctcgcctt ccgcaacgac ccgaagacgg tgcgcacgtt catggcgatc tcgaccatca
     2521 ccgtgtccac cttctacctc accgcgcaga tggtgggcgg cggcgtgctg gtgaagaccc
     2581 tcatcggaat cgactacgaa tactcggtca tgacggtggg cgtgctcatg ctcgcctacg
     2641 tggtgttcgg cggcatggtc gcgacgtcgt atgtgcagat cgtgaaggcc gttctcctcg
     2701 tgatcgcctc cctgctcatg gtcatcatgg tgtggagcca gtacggcttc agcctgcccg
     2761 gcttcctgca ggcggcggtg aacgacccca acatccaggg gcgcgtcgcg acgatgctgg
     2821 gcgactcggc caggaacatg acgcccgccg agctcggcca gcgcttcctc gagccgggcc
     2881 tgctgttcaa gaacccgatc gaccagatct cgctcggcat ggcgctcgtg ctcggcacgg
     2941 cgggcctgcc gcacatcctg atgcgcttct tcacggtgcc gaccgcgcag gccgcgcgca
     3001 agtccgtgat ctgggcgatg gcgatcatcg gcggcttcta cgtcctcacc ctgttcctgg
     3061 gcctgggcgc ggccatgaag gtgggtcctt cgggcatcac gctgatcgac aagggcggca
     3121 acatggccgc cccgctcctg gcgcagtacc tcggcggcgg cgcggactcg atgctcggca
     3181 acctgatgct cgcgttcgtg gcggcggttg cctt
//
LOCUS       DASUEV010000182         5447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1177424, whole genome shotgun sequence.
ACCESSION   DASUEV010000182 DASUEV010000000
VERSION     DASUEV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177424"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="proB"
                     /locus_tag="VFV55_05935"
     CDS             complement(<1..953)
                     /gene="proB"
                     /locus_tag="VFV55_05935"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX4943874.1"
                     /translation="MRDELAGCRRVVVKIGSSLLTNAGAGLDRDAVSDWAGQIAALRA
                     RGVEVLVVSSGAIAEGMKRLGWMKRPRAVNELQAAAAVGQMGLVQMWESCFAERDLRT
                     AQVLLTHEDLGDRRRYLNARTTLKTLVSLGVVPVINENDTVVVDEIRFGDNDTLASLV
                     TNLIEADALAILTDQKGLYSADPRKDPSATLVDRAHAGDPALEVMAGGAGSDIGSGGM
                     ITKVLAAKRAARSGAHTVIAWGREPDLLTRVFSGEEIGSFLIAATPPVAARKQWLADH
                     LKPAGRITLDAGAVKALTADGKSLLAIGATAVAGSFERGEVV"
     gene            complement(946..1965)
                     /gene="obgE"
                     /locus_tag="VFV55_05940"
     CDS             complement(946..1965)
                     /gene="obgE"
                     /locus_tag="VFV55_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX4943875.1"
                     /translation="MKFFDEARIEVIAGDGGNGSAHFRREKFVPRGGPDGGDGGRGGS
                     IIAVADFNLNTLIDYRYTRIFRAKHGEDGSGRDRYGHGADDIILRVPVGTVIRDADTG
                     ETIADLAKSGDQAVLAKGGKGGLGNIHFKSSINRAPRQFTPGETGERRNLEMELRVLA
                     DVGLAGMPNAGKSTFVRAVSAARPKVADYPFTTLNPSLGVVRVDMNRSFVIADIPGLI
                     EGAAEGAGLGHQFLRHLQRTKLILHLVDFAPFDDAVDAVKEARAIAQELKKYDPALYK
                     KPRWLVLNKADLLDPAERDKRAKAFVKKLAWKGPWFMVSAINGEGTRELCFAIMDFLE
                     GKRDA"
     gene            complement(1962..2591)
                     /locus_tag="VFV55_05945"
     CDS             complement(1962..2591)
                     /locus_tag="VFV55_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX4943876.1"
                     /translation="MPDLALFLVASALLTIAPGPDIVYVLTRGVSQGRKAGLAAALGF
                     ATGCIFHTLLAALGIAALIRSSDLAFNAVRWAGAAYLVWIGIHALRHRASFSLAGGGD
                     AKALAAIYRQSVIGNALNPKVTLFFLSFLPQFVDARSGGVAWQMAFLGVVFMAQTVVI
                     FGAVALFSGWIGERLRAHPAVGEKLGLFAGLTFIALGIRVAMPDMGARP"
     gene            complement(2659..2922)
                     /gene="rpmA"
                     /locus_tag="VFV55_05950"
     CDS             complement(2659..2922)
                     /gene="rpmA"
                     /locus_tag="VFV55_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX4943877.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGLKAFGGELVNAGSILVRQRGTK
                     FHPGQHVGIGKDHTLFALLTGKVKYGQHGPRQKNTVSIEPAQA"
     gene            complement(2935..3246)
                     /gene="rplU"
                     /locus_tag="VFV55_05955"
     CDS             complement(2935..3246)
                     /gene="rplU"
                     /locus_tag="VFV55_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX4943878.1"
                     /translation="MYAVIKTGGKQYRVAPGESLKIEQVQADVGATLVLDQVLMVADG
                     EAVKVGTPTLAGANVSATVVAHGRGPKVRIFKMRRRKHYQKTQGHRQNYTEIRVDAIN
                     Q"
     gene            3400..4368
                     /locus_tag="VFV55_05960"
     CDS             3400..4368
                     /locus_tag="VFV55_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX4943879.1"
                     /translation="MSLESIRAPIAADLQAVDAVIRRRLDSEVALVRTIADYIIAAGG
                     KRLRPALLLLAANAVGANGPIRHELATVIEFIHTATLLHDDVVDESSLRRGRRTANAE
                     FGNAASVLVGDFLYSRAFQMMVSVGHLRVMQVLSDATNVIAEGEVLQLLNVNDPDTDE
                     ESYLRVIRYKTAKLFEAATQVGAILGGAPPELERALAEYGMHVGTAFQVIDDVLDYSG
                     DLAETGKNLGDDLAEGKPTLPLIRAMQVGDAGERALVRRAIEEGGKADLVAVVAAINR
                     TGALDYSRDCARKEAAIATARLDALPDSACKDSLLQLAHFSVERKF"
     gene            4410..4486
                     /locus_tag="VFV55_05965"
     tRNA            4410..4486
                     /locus_tag="VFV55_05965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4444..4446,aa:Pro,seq:cgg)
     gene            4621..>5447
                     /locus_tag="VFV55_05970"
     CDS             4621..>5447
                     /locus_tag="VFV55_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4943880.1"
                     /translation="MNLRQIEAFKAVMETGTVTMAAARLHISQPAVSKLLQLFERGAR
                     MKLFVRDRGRLSPTPEARLLYDEVERVFQGAGRIRQAAEEIRSLERGSLAVGAMPALS
                     VGFVQEMLARLGTEHPGVVVQVHSRDTPKLVEQLVTHQIELVLSVLPMDHPEIRTESL
                     CRVPLVCILPPGHRLSGRREIRCRDLAGERFASFRHDSAVRRRIDQAFEDAKVIQRIV
                     LEAPMAPTICAFVARGLGVSIVNPLYVGAFAPALVVRPFRPRIESEISMAMPRGRRLS
                     "
BASE COUNT          823 a         1903 c         1829 g          892 t
ORIGIN      
        1 accacttcgc cgcgctcgaa cgagccggcg accgcggtcg ccccgatggc gaggaggctc
       61 ttgccatcgg cggtcagcgc cttcaccgcg cccgcgtcca gcgtgatgcg cccggcgggc
      121 ttcaggtggt cggccagcca ctgcttgcgc gccgccaccg gcggcgtggc cgcgatgagg
      181 aacgagccga tctcctcgcc ggagaaaacg cgcgtgagca ggtcgggttc ccggccccat
      241 gcgatcaccg tgtgcgcgcc gctgcgggcg gctcgcttcg cggccagcac cttcgtgatc
      301 atgccgccgc tgccgatgtc cgagccggcg ccccccgcca tcacctccag tgcgggatcg
      361 ccggcgtggg cgcggtcgac gagcgtggcc gacgggtcct tgcgcggatc cgccgagtac
      421 agtcccttct ggtcggttag gatcgcgagc gcgtcggctt cgatgaggtt ggtcaccagc
      481 gaggcgagcg tgtcgttgtc gccgaagcgg atctcgtcca cgaccacggt gtcgttctcg
      541 ttgatgaccg gcaccacgcc cagcgaaacg agcgtcttca gcgtggttcg cgcattgagg
      601 tagcggcgcc ggtcacccag gtcctcgtgc gtgaggagca cctgcgcggt ccgcaggtcc
      661 cgttcggcga agcacgactc ccacatctgc accagtccca tctggccaac cgcggccgcg
      721 gcctgcagct cgttcaccgc gcgcgggcgt ttcatccagc ccaggcgctt catgccctcg
      781 gcgatcgcgc ccgaggagac gacgagcacc tccacgcccc gtgcgcgaag cgccgcgatc
      841 tgtcccgccc agtcggaaac ggcgtcgcgg tcgagcccgg ctcccgcatt ggtgagcagg
      901 ctcgagccga tcttcaccac gacccgcctg cagccggcaa gctcgtcacg catcgcgctt
      961 gccttcgagg aaatccatga tcgcgaagca gagctcgcgg gttccctcgc cattgatggc
     1021 ggagaccatg aaccacgggc ccttccaggc cagcttcttc acgaatgcct tcgcgcgctt
     1081 gtcgcgctcc gcggggtcga ggagatcggc cttgttgagc accagccagc gcggcttctt
     1141 gtacagggcc ggatcgtact tcttcagttc ctgcgcgatc gcccgggctt ccttcaccgc
     1201 gtccaccgcg tcgtcgaatg gcgcgaagtc cacgaggtgc aggatgagct tcgtgcgctg
     1261 caggtggcgc aggaactggt gcccgagccc cgcgccctcg gccgcgccct cgatgaggcc
     1321 cgggatgtcg gcgatcacga acgagcggtt catgtcgacg cgcaccaccc ccaggctggg
     1381 gttgagggtc gtgaagggat agtccgccac cttggggcgc gcggccgaga cggcccgcac
     1441 gaacgtcgac ttgccggcgt tgggcatgcc ggccaggccc acgtcggcaa ggacgcgcag
     1501 ctccatctcg aggttcctgc gctcgccggt ctcgccgggg gtgaactgcc gcggcgcgcg
     1561 gttgatgctc gacttgaagt ggatgttgcc gagcccgccc ttgccgccct tcgcgagcac
     1621 ggcctggtcg ccggatttcg cgaggtcggc gatcgtctcc cccgtgtcgg cgtcgcggat
     1681 caccgtgccg accgggacgc gcaggatgat gtcgtcggcg ccgtgcccgt agcggtcgcg
     1741 gccgctgccg tcctcgccgt gcttggcgcg gaagatgcgc gtgtagcggt agtcgatgag
     1801 cgtgttgagg ttgaagtcgg ccaccgcgat gatggagccg ccgcggccgc cgtcgccgcc
     1861 gtccggcccg ccgcggggca cgaacttctc gcgccggaag tgggcggagc cgttgccgcc
     1921 atcgccggca atgacctcga tgcgggcttc gtcgaagaat ttcacgggcg tgcccccatg
     1981 tcgggcatcg ccacgcggat gcccagcgcg atgaaggtga ggcccgcgaa gaggccgagc
     2041 ttctcgccca cggccggatg ggcgcgcagc cgctcgccga tccaccccga gaagagcgcc
     2101 accgccccga agatgacgac ggtctgcgcc atgaagacga cgccgaggaa cgccatctgc
     2161 catgccacac cgcccgagcg ggcatcgacg aactgcggca ggaaggaaag gaagaagagc
     2221 gtcaccttgg ggttgagggc gttgccgatc acgctctgcc ggtagatcgc ggcaagcgcc
     2281 ttcgcatcgc ctccgcccgc cagcgagaac gaggcgcggt ggcgaagcgc gtgaatgccg
     2341 atccagacga gataggccgc gcccgcccag cgcactgcgt tgaacgcgag gtcggaggag
     2401 cggatgagcg ccgcgatgcc cagtgcggcc agcagggtgt gaaagatgca gccggtggcg
     2461 aacccgagcg ccgccgcgag cccggccttg cgcccctggc tcacgccgcg cgtgagcacg
     2521 tagacgatgt cgggcccggg cgcgatggtg agcagcgcgc tggcgacgag gaagagggcg
     2581 aggtcgggca tgtcttcttc tcccggaaac aaaaaagccc cgtacgatga cggggctctt
     2641 tccggaagaa aggagccgtc aggcctgcgc cggctcgatg ctcacggtgt tcttctgcct
     2701 gggtccgtgc tggccatact tcaccttgcc ggtgagcagc gcgaagaggg tatggtcctt
     2761 gccgatgccc acatgctggc ccggatgaaa cttcgtgccg cgctggcgga cgaggatcga
     2821 acccgcgttc acgagttcgc cgccgaaggc cttgaggccc aggcgcttgc tttcggaatc
     2881 gcggccgttg cgggatgagc cgcctgcttt cttgtgtgcc atgatcgcta cccgttactg
     2941 gttgatggcg tccacacgga tttccgtgta gttctgccgg tggccctgcg ttttctgata
     3001 gtgcttgcgg cggcgcatct tgaagatgcg gaccttgggc ccccggccgt gggcgaccac
     3061 ggttgcggag acattcgcgc cggcaagcgt cggcgtgcca accttcacgg cctcgccatc
     3121 cgccaccatc agcacctggt cgagcacgag ggtggcgccg acgtcggcct gcacctgctc
     3181 gatcttgagg ctctctcccg gggcaacccg atactgcttg ccgccggtct tgatgaccgc
     3241 gtacatatct ggaactcctg ttttagtttg acaattcacg gccttttgca ccgcgaaccc
     3301 gctataatac gcgaaatccc aagcaacgtc aaagacttgt gctcgggccc ggccgggccc
     3361 gacagccccg gaaccccctt gaaccagtcc cacatccccg tgagcctcga atcgatccgg
     3421 gcgcccatcg ctgccgacct gcaggccgtg gacgccgtca tccggcgccg gctcgattcc
     3481 gaggtggccc tcgtccggac catcgccgac tacatcatcg ccgccggcgg caagcgcctg
     3541 cgcccggccc tgctcctgct cgccgccaac gccgtggggg ccaacggccc cattcgccac
     3601 gagctcgcga ccgtcatcga attcatccac acggcgaccc tgctccacga cgacgtcgtc
     3661 gacgagtcga gcctgcggcg cggccggcgc acggcgaatg ccgaattcgg caacgccgcc
     3721 tcggtgctgg tgggcgactt cctctactcg cgcgccttcc agatgatggt gtcggtgggc
     3781 cacctgcgcg tgatgcaggt gctttcggac gcgaccaacg tcatcgccga aggcgaggtc
     3841 ctgcaactgc tcaacgtgaa cgacccggac accgacgagg aaagctactt gcgcgtgatc
     3901 cgctacaaga cggcgaagct cttcgaggcc gccacccagg tgggcgcgat cctcggcggc
     3961 gcccccccgg aactcgagcg cgcgctggcc gagtacggca tgcacgtggg caccgccttc
     4021 caggtcatcg acgatgtcct cgactattcc ggcgatctgg ccgagacggg caagaacctg
     4081 ggcgacgatc tcgccgaggg caagcccacg ctgccgctca tccgagcgat gcaggtgggc
     4141 gatgccgggg agcgggcgct cgtgcgccgg gccatcgagg aggggggaaa ggccgatctc
     4201 gtcgcggtgg tcgccgccat caatcgaacc ggcgccctcg actactcgcg cgattgcgcc
     4261 cgcaaggagg ccgccatcgc cacggcccgg ctcgacgcac tgccggattc cgcgtgcaag
     4321 gacagtctgc tacaattggc ccacttttcc gtcgagcgga agttctaggc gacggatttc
     4381 gacgggcgcg aaggcgcccg tttcggcctc ggggtgtagc ttagcctggt agagcgctac
     4441 gttcgggacg tagaggccgg aggttcaaat cctctcaccc cgaccattcc cttcccgcaa
     4501 agcgccggcc tcgcatggag gccgttgcgc tgggtgctct ccccgccatg gcagtcgtgg
     4561 tcccggtggg cgatagccag ggggcgtact gtcaccgaac cgtgcgtaag ggccgttgac
     4621 gtgaacctgc gccagatcga agccttcaag gcggtcatgg agaccggcac ggtcaccatg
     4681 gccgcagcgc gcctgcacat ctcgcagccg gccgtgagca agctgctgca gcttttcgaa
     4741 cgcggcgcgc gcatgaaact cttcgtccgc gaccgcgggc gcctgtcgcc cacgccggag
     4801 gctcgactgc tctacgacga ggtggagcgc gtcttccagg gcgcgggccg catccgccag
     4861 gcggcggagg agatccgctc gctcgaacgc gggtcgctcg ccgtgggcgc gatgccggcg
     4921 ctgtcggtgg gcttcgtgca ggagatgctc gcccgtctcg ggaccgagca tcccggcgtc
     4981 gtcgtccagg tgcattcgcg cgacaccccg aagctcgtcg agcagcttgt cacgcaccag
     5041 atcgaattgg tcctgtccgt gctgcccatg gaccacccgg aaatccgcac cgagtcgctc
     5101 tgccgggtgc cgctcgtctg catcctgccg ccgggccatc gcctctccgg gcggcgcgaa
     5161 atccgctgcc gcgaccttgc cggggagcgc ttcgcctcgt tccgccacga ttccgcggtg
     5221 cggcgacgca tcgaccaggc cttcgaggat gcgaaggtca ttcagagaat cgtgctcgag
     5281 gcgcccatgg cccccacgat ctgcgccttc gtggcgcgcg gcctgggcgt gtcgatcgtg
     5341 aacccgctct acgtcggggc cttcgccccg gcgctcgtgg tgcggccctt ccgcccgcgc
     5401 atcgagtcgg aaatctcgat ggcgatgccc cggggccgcc gcctttc
//
LOCUS       DASUEV010000183         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_56303, whole genome shotgun sequence.
ACCESSION   DASUEV010000183 DASUEV010000000
VERSION     DASUEV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56303"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="phaZ"
                     /locus_tag="VFV55_05975"
     CDS             complement(<1..1145)
                     /gene="phaZ"
                     /locus_tag="VFV55_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX4943881.1"
                     /translation="MLYQFYDWQREAMEPWRRLAQAANEFYGHPVSPFAYLPGSRNVA
                     AAFDLMGRLTQRYERAEFGIGSVRVGERNCAVTEVFELDRPFCRLLHFVKQGAPAQPR
                     VLVFAPLSGHFATLLRDTVNSLLSDHDVWITDWIDAKEVPLAEGPFHFDDYVDYVRDF
                     IGFVGPGVHAISVCQPTVPVLAGVSLMAAAGETVPRSLTMMGGPIDTRSSPTAVNDFA
                     KHRPISWFEAKVVQRVPMRYPGFMRRVYPGFLQFAGFVAMNPDRHFESHLQYYQHVIQ
                     GDGEGAGAHRRFYDEFNAVMDLPAEYYLDTVERVFHKHLLPRGKLQVRGERVRPELIR
                     DSALFTIEGELDDISGPGQTKAALALCKGIPAKAKMHFRAPGVGHYG"
     gene            1261..2241
                     /gene="pip"
                     /locus_tag="VFV55_05980"
     CDS             1261..2241
                     /gene="pip"
                     /locus_tag="VFV55_05980"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX4943882.1"
                     /translation="MPPEAHRKDGESLPLYPAADSRRGGFLDVGGGHRLYWEESGNAE
                     GVPVVFLHGGPGAGASPVHRQFFDPAHYRIVIFDQRGAGRSIPLGSLECNTTPDLVDD
                     MERLREHLGIARWIVFGGSWGSTLSLAYAERHRARCLALVLRGIFLGRPAEIDWFLYG
                     IRNVYPEPWHDFAGFIPEAERGDLLAAYHRRLIDPDPAVHMPAARRWSVYEGSCSTML
                     PNPALVADFASDRVALGLARIEAHYFANRLFLPDDFLLANIDRIRDVPAVIVQGRYDA
                     VCPPVTANDLHRAWPEAEYHVVPDAGHSAFEPGIRSRLVAAMEKFKTIGE"
BASE COUNT          399 a          794 c          799 g          376 t
ORIGIN      
        1 atcccgtagt ggccgacgcc gggggcccgg aagtgcatct tcgccttcgc cggaatgccc
       61 ttgcacaacg cgagcgccgc tttcgtctgc ccgggcccgg aaatgtcgtc gagctcgccc
      121 tcgatggtga agagggccga gtccctgatg agttcggggc gcacgcgctc gccgcgaacc
      181 tggagctttc cgcgcggcag aaggtgcttg tggaagacgc gctccaccgt atcgaggtag
      241 tactcggcgg gcaggtccat caccgcgttg aactcgtcgt agaagcgccg gtgggcgccc
      301 gcgccctcgc catcgccctg gatgacgtgc tggtagtact gcaggtgcga ttcgaaatgg
      361 cggtccgggt tcatggcgac gaacccggcg aactgcagga aacccgggta gacgcgccgc
      421 atgaagccgg ggtagcgcat cggcacgcgc tgcacgacct tggcctcgaa ccacgagatc
      481 ggccggtgct tggcgaagtc gttcaccgcg gtggggctgg agcgcgtgtc gatggggccg
      541 cccatcatgg tgagcgaccg cggcaccgtc tcgcccgccg ccgccatgag cgacacgccc
      601 gcgagcacgg gcacggtcgg ctggcatacg gagatcgcat gcaccccggg gcccacgaag
      661 ccgatgaagt cgcgcacgta atcgacgtag tcgtcgaaat ggaagggccc ttcggcaagg
      721 ggcacctcct tcgcgtcgat ccagtcggtg atccacacgt cgtggtcgga gagaagggaa
      781 ttgaccgtat cgcgaagcag cgtggcgaag tgcccggaaa gcggcgcgaa cacgagcacc
      841 ctgggttgcg ccggcgcgcc ttgcttcacg aagtgaagca ggcggcagaa gggcctgtcc
      901 agctcgaaca cctcggtgac cgcgcagttg cgctcgccga cacgcacgct gccgatcccg
      961 aactcggcgc gctcatagcg ctgcgtgagg cggcccatga ggtcgaaggc ggcggcaacg
     1021 ttgcggctgc cgggcaggta ggcgaaggga ctcaccgggt ggccatagaa ttcgttggcc
     1081 gcctgcgcca gcctgcgcca cggctccatc gcctcgcgct gccaatcgta gaactggtag
     1141 agcacccccg gcctccgtca atgctgcatt gcggcatagt gtagcgtttt tccaacggcg
     1201 ggaagattga tgcggagcaa gaacgccggg gcgcatcttc gctctggtaa gctcctcgcc
     1261 atgcctcccg aagcccaccg gaaagacggc gaatccctgc cgctctaccc cgccgcggac
     1321 tcccgccgcg gggggttcct cgacgtgggc ggcggccacc ggctgtactg ggaggaaagc
     1381 ggaaatgccg agggcgtgcc ggtcgtcttc ctgcacggcg gacccggcgc cggcgcgagc
     1441 ccggtgcacc gccagttctt cgacccggcc cactatcgaa tcgtcatctt cgaccaacgc
     1501 ggcgccggcc gctcgattcc gctgggaagc ctcgagtgca acacgacgcc cgacctcgtg
     1561 gacgacatgg agcgcctgcg cgagcacctg ggcatcgcgc gctggatcgt cttcggcggc
     1621 tcgtgggggt cgacgctttc gctcgcctac gcagagcgcc atcgcgcgcg ctgcctggcc
     1681 ctcgtgctgc gcggcatctt cctgggacgg cccgccgaga tcgactggtt cctgtacggg
     1741 attcgcaacg tctacccgga gccgtggcac gatttcgcgg ggttcatccc cgaggcggag
     1801 cgcggcgacc tcctcgcggc ctaccaccgc cggctcatcg atcccgatcc cgcggtgcac
     1861 atgccggcgg cgcgacgctg gagcgtctac gagggatcgt gctcgacgat gcttcccaac
     1921 ccggcgttgg tggcggactt cgcctcggat cgcgtggcgc tggggctggc gcgcatcgag
     1981 gcgcattact tcgccaatcg cctcttcctg ccggacgatt tcctgctcgc gaacatcgac
     2041 cgcatccgcg atgtcccggc ggtgatcgtg cagggacgct acgacgcggt gtgccccccg
     2101 gtcaccgcga acgacctgca ccgtgcgtgg ccggaggccg aataccacgt cgttcccgac
     2161 gcggggcact ccgcctttga acccggcatc cgctcgcggc tcgttgccgc gatggagaag
     2221 ttcaagacca tcggcgagtg acgccaaggc aaacccttgg aacacggatg aacacggatg
     2281 aacacggatg aacacggatg aacacggatg aacacggatg aacacgaata aggccatgaa
     2341 tcttcctgtc cgggctggcg gtgacgca
//
LOCUS       DASUEV010000184         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_271983, whole genome shotgun sequence.
ACCESSION   DASUEV010000184 DASUEV010000000
VERSION     DASUEV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271983"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /gene="rplD"
                     /locus_tag="VFV55_05985"
     CDS             complement(<1..239)
                     /gene="rplD"
                     /locus_tag="VFV55_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX4943883.1"
                     /translation="MKVQLMNGTGSASELQVSDEVFAAAFNEPLVHQVVVAYQATGRQ
                     GTRKQKTRSEVRGGGKKPWRQKGTGQARAGTIRSPL"
     gene            complement(249..920)
                     /gene="rplC"
                     /locus_tag="VFV55_05990"
     CDS             complement(249..920)
                     /gene="rplC"
                     /locus_tag="VFV55_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX4943884.1"
                     /translation="MALGLVGRKIGMTRLFSANGEGTPVTVLEVTANRVTQVKDEAKD
                     GYRAVQLTIGKRRADKLPKPLAGKYAKAGVEPGRGLWEFRLGADEGADLKPGAEVKLD
                     IFQVGGHVDVQGTSIGKGFAGTIKRHHFRGGRATHGNSLSHRAPGSIGQRQTPGRVFP
                     GKKMAGHLGDATATCLNLEVMQIDAERNLLMVKGAVPGPKGQDVVIRPSVKIKPKAKT
                     AKAEK"
     gene            complement(930..1241)
                     /gene="rpsJ"
                     /locus_tag="VFV55_05995"
     CDS             complement(930..1241)
                     /gene="rpsJ"
                     /locus_tag="VFV55_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX4943885.1"
                     /translation="MANQKIRIRLKAFDHRLIDRSAAEIVDTAKRTGALVHGPIPLPS
                     KVERYTLNRSPHCDKKSRDQFEVRTHKRIMDIIEPTDKTVDALMKLDLAAGVDVEIKL
                     S"
     gene            complement(1259..2449)
                     /gene="tuf"
                     /locus_tag="VFV55_06000"
     CDS             complement(1259..2449)
                     /gene="tuf"
                     /locus_tag="VFV55_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX4943886.1"
                     /translation="MSKEKFSRTKPHLNVGTIGHVDHGKTTLTAALTKVLAAKFGGEV
                     RNYDQIDNAPEERERGITISTTHVEYQSDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKADMVDDKDLLELVQEEVKDLLTK
                     YGFPGDKTPIVIGSALKALEGDQSELGEPSIVKLSEALDSYIPQPQRAIDGPFLMPIE
                     DVFSISGRGTVVTGRIERGIVKVGDEIEIVGIKPTTKTTVTGVEMFRKLLDQGEAGDN
                     VGVLLRGTKREEVERGQVLAKPGSITPHTKFEAEVYVLTKEEGGRHTPFFQGYRPQFY
                     FRTTDVTGSCELPSGTEMVMPGDNIKMVVTLINPIAMEQGLRFAIREGGRTVGAGVVA
                     KVIE"
     gene            complement(2473..>2753)
                     /gene="fusA"
                     /locus_tag="VFV55_06005"
     CDS             complement(2473..>2753)
                     /gene="fusA"
                     /locus_tag="VFV55_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009586196.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX4943887.1"
                     /translation="LEPIMDVEVTSPPDYLGNVMGDLNSRRGIIMSQEDGHGNVKVIR
                     AEVPLANMFGYSTSLRSATQGRATYTMEFKKYAPVPSNVAESVMKKAS"
BASE COUNT          398 a          851 c          904 g          600 t
ORIGIN      
        1 agcgggctgc ggatcgtgcc cgcgcgcgcc tggccggtgc ccttctggcg ccacggcttc
       61 ttgccgccgc cacgcacttc ggagcgggtc ttctgcttgc gcgtgccctg gcggccggtt
      121 gcctgatagg ccaccaccac ctgatgcacc agcggctcat tgaaggccgc ggcaaatacc
      181 tcatcggaca cctgaagctc cgaggccgat ccggtaccgt tcataagttg cactttcatg
      241 tccgttcctt acttctcggc cttggccgtc ttggccttag gcttgatctt gacgctcggg
      301 cggatcacca cgtcctggcc cttgggtccg gggaccgcgc ccttgaccat gagcagattg
      361 cgttcggcat cgatctgcat gacctcgaga ttcaggcagg tcgccgtggc gtcgcccagg
      421 tgcccggcca ttttcttgcc cggaaacacg cggcccggcg tctggcgctg gccgatcgag
      481 cccggggccc ggtggctcag cgagttaccg tgcgtggcgc gaccgccgcg gaaatgatgg
      541 cgcttgatcg tgccggcaaa gcccttgcca atagacgtgc cctgcacgtc cacgtgcccg
      601 cccacctgga agatatccag cttgacctcg gcgcccggtt tgagatcggc accttcgtcg
      661 gcaccgaggc ggaattccca cagcccgcgg cccggctcca caccggcctt ggcgtacttg
      721 cccgccagcg gcttgggcag cttgtcggcg cggcgcttgc cgatggtcag ctgcaccgcg
      781 cgatagccgt ccttcgcctc gtccttcacc tgggtgacgc ggttggcggt cacttccagc
      841 acggtgacgg gtgtgccctc gccgttcgcc gagaacaggc gggtcatacc gatttttctg
      901 cctacgagtc cgagtgccat gttctagtct caggaaagct tgatttcgac gtccacgccg
      961 gccgcgaggt cgagtttcat cagcgcgtcc acggtcttgt ccgtgggctc gatgatgtcc
     1021 atgatccgct tgtgcgtgcg cacctcgaac tggtcgcgcg atttcttgtc gcaatgcggc
     1081 gaacggttga gcgtgtaacg ctccaccttc gacggcagcg gaatcgggcc gtgcacgagc
     1141 gcgccggtgc gcttcgccgt gtcgacaatt tccgccgcgg aacggtcgat caaacggtga
     1201 tcgaaggcct tgaggcggat gcggatcttc tggttagcca tattgcttcg ctcgaatcct
     1261 attcaatcac cttcgccacc acccccgcgc ccacggtccg gccgccttcg cgaatggcga
     1321 agcgcaggcc ctgttccatg gcgatggggt tgatcagggt gacgaccatc ttgatgttgt
     1381 cgcccggcat gaccatctcg gtgccgctcg gcagttcgca ggagccggtg acgtcggtgg
     1441 ttctaaagta gaactgggga cggtagccct ggaagaacgg ggtgtgtcgt ccgccttctt
     1501 ccttggttaa tacgtacacc tcggcctcga atttggtgtg gggcgtgatg gagccgggct
     1561 tggccaggac ctggccgcgt tcgacttctt cgcgcttggt gccgcgcagg agcacgccga
     1621 cgttgtcgcc ggcctcgccc tggtcgagca gcttgcggaa catttccacg ccggtgacgg
     1681 tggtcttggt ggtgggcttg atgccgacga tctcgatttc atcgcccact ttgacgatgc
     1741 cgcgttcgat tcttccggtg acgacggtgc cgcggccgga gatggagaac acgtcttcga
     1801 tgggcatgag gaaggggccg tcgatggcgc gctggggctg cgggatatag gagtccaggg
     1861 cctcggacag tttcacgatc gagggttcgc cgagttcgga ctggtcgcct tcgagggcct
     1921 tcagggcgct gccgatgacg atcggggtct tgtcgccggg gaagccgtat ttggtgagca
     1981 ggtctttgac ttcttcctgg accagttcca gcaggtcctt gtcgtcgacc atgtcggcct
     2041 tgttcaaata cacgacgatg tagggcacgc cgacctggcg cgccagcagg atatgttcgc
     2101 gggtctgggg catggggccg tcggcggccg acaccaccag gatggcgccg tccatctggg
     2161 cggcaccggt gatcatgttc ttgacgtagt cggcgtgtcc cgggcagtcg acgtgggcgt
     2221 agtggcgctt gtcactctgg tactccacgt gggtggtgga gatggtgatg ccgcgctcgc
     2281 gttcttccgg ggcgttgtcg atctggtcgt agttccggac ctcgccgccg aacttggcgg
     2341 ccagcacctt ggtcagggcc gcggtgagcg tggtcttgcc gtggtcgacg tggccgatgg
     2401 tgccgacgtt gaggtgcggt ttggtgcggg agaatttttc cttggacacg ggatgcctct
     2461 atcttgaatt agttaactgg ccttcttcat gacggactcg gcgacgttcg acggcaccgg
     2521 agcgtatttc ttgaattcca tcgtgtaggt cgcgcgaccc tgggtcgccg agcgcaggct
     2581 cgtcgagtaa ccgaacatgt tcgccagcgg cacctcggcg cggatgacct tcacgttgcc
     2641 gtgaccgtct tcctggctca tgatgatgcc gcggcgcgaa ttcaggtcgc ccatcacgtt
     2701 gcccagatag tcgggcggcg aggtcacttc cacgtccatg atcggctcca gca
//
LOCUS       DASUEV010000185         7795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_272592, whole genome shotgun sequence.
ACCESSION   DASUEV010000185 DASUEV010000000
VERSION     DASUEV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272592"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1213
                     /locus_tag="VFV55_06010"
     CDS             89..1213
                     /locus_tag="VFV55_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4943888.1"
                     /translation="MTAKARILVVDDVPNNVKLLADLLAVRGYEVLTACDGDEALERV
                     KDSRPDLVLLDVMMPRMNGYDTCRAIRADPATGILPIVMVTALDPAGERIKGIEAGAD
                     DFLTKPINVQELLARVKSLLRVQSLFQEVARQKSELAEWNRTLEARVAEQVAQVERLS
                     RLRRFLSPKVADLIVAGRLDDPLATRRREVVIVFVDLRGFTAFTETADPEEVFAVLRA
                     YHAGIGRLIARHEGTIEHFAGDGVMIVFNDPAPLPDPAFSAVSFAIDARDAVAELAEG
                     WKKRGYGLGCGVGIAQGYATIGTIGYEGRYDYGTIGTVANLAARLCGEAKAGEILVAR
                     KVHGLVEDRVLAGPSRDLALRGFHEPVAACSVIGRKEGTS"
     gene            1210..1860
                     /locus_tag="VFV55_06015"
     CDS             1210..1860
                     /locus_tag="VFV55_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4943889.1"
                     /translation="MTTRNARAEAPFILTPEELAAISSNAPARHYPPKTIIVTEGDQA
                     GALFVILEGSCKAYVSGEDGREAVLSVMGPGEYFGEIALDEGPRSASVMTLEPSRLLV
                     VPQEGFQAFLAGNPDFAAHFIRKLIGRIRELTRIVGNLSLLDVYGRVARLLLDSAREE
                     EGLLVIPERLTQIDIASRVGCSREMVSRIFKDLVQGGYVSLEKERILIHRRPPARW"
     gene            1952..2230
                     /locus_tag="VFV55_06020"
     CDS             1952..2230
                     /locus_tag="VFV55_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943890.1"
                     /translation="MKALFDRYLQKKIGLNYHTAYHIDEATLTLAADTYFAALGERDG
                     ATHYYPYSSVIHVMEKAGGVEVGGLFTHKHAWPLVVKVGHVVDVGPMV"
     gene            complement(2246..2491)
                     /locus_tag="VFV55_06025"
     CDS             complement(2246..2491)
                     /locus_tag="VFV55_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943891.1"
                     /translation="MNDRTVRDAFMEVVVNQLDTGDPPETKATLDRLVAAGSTNAEAM
                     QLIAAVARNEMQAMLAAGRAFDNARYAKLLAKLPELD"
     gene            complement(2488..3177)
                     /locus_tag="VFV55_06030"
     CDS             complement(2488..3177)
                     /locus_tag="VFV55_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943892.1"
                     /translation="MTGICLDDRTAPVDRRRPWLAALGVAVALPVAALALYAGFGKPQ
                     AIESSRAFGTLAGPLTAAQAPAFRDQLARHLADNPRDARAWALLGRVELALDRFAESE
                     AAFENAVADPKVALDPGIWCDYADAAGLAQGGRLEGKPARLIARALDLDGSHPRALDM
                     AGSLAIEQGHHANAARHWRSLLDQLGATDPRRAELARAVERVERLAVPGMTKPGARRN
                     MQVSDPQGRGS"
     gene            complement(3188..4930)
                     /locus_tag="VFV55_06035"
     CDS             complement(3188..4930)
                     /locus_tag="VFV55_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX4943893.1"
                     /translation="MNSIRSRAIVRWAFGLLAVFALCSAQAAPAAAEKPAAKPAAPAK
                     VATGKAPAANRECLDCHEELKEFHDPGKHKGVACTECHTGTDAHLKKKSTRPVTITDP
                     AKCGSCHRNQFETMYKMNFAKTARNEKSQLTGVSPNPAWEKLMGPHGFTKEHNMPRSH
                     AFALYDQLVVDRAFGGRFENKYGWGDLARAGGNFNIWDVLVDKYPGEPHKAFRPGTAA
                     AANPVCMSCKTADHILDWAYMGDPDPKAKWSRLSKVNEFVKDTRHSLSCIFCHDPHGA
                     QPRIIRDGLIQALTRPEKDTLWHKDAKAGKVDVKELGQRGYTRKIAILDKYDSKLQCG
                     QCHVEYNCNPGTDSATGQPVTMADQRTNHFPLKNVNDLAQHYRDLKFRDFKHAITGAL
                     LWKAQHPDTEVYYGSVHDREGAMCHTCHMPKMKDAKTGKAFTSHWQTSPKEYVKEACL
                     GCHKTWNREQAVYTIDSLRNRYQGKLRKAEYWLTRMIDKFEEAKNLGVDEATLNVVRE
                     KHYEAHVHWEFWTASNGSYFHNPKLADESINKGMVISQEAIKTLEDAMGKKRTIAAAP
                     AAVPASAPAAAAPK"
     gene            complement(5009..5776)
                     /locus_tag="VFV55_06040"
     CDS             complement(5009..5776)
                     /locus_tag="VFV55_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX4943894.1"
                     /translation="MCLIAIAWQAHPEFPLVVAANRDEWRDRPATPADWWKDHPRLLA
                     GRDLQAGGTWMGITRAGRFAAVTNFRDPSERRSTALSRGALVAEFLLGTDSPMRYLAG
                     LASRAAQYNGFNLLVSDGSSLAYFGSREGEVRDVEPGVHALSNHLLDEPWPKVAKARQ
                     AMREAVDDGDEPLFAMLSDTTPAPDSQLPDTGVGLERERMLSPVLIVGADYGTRASTV
                     LRAGHAAARFEERTRDATGAVTVTRSFDFRFDASQHR"
     gene            5834..7243
                     /locus_tag="VFV55_06045"
     CDS             5834..7243
                     /locus_tag="VFV55_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX4943895.1"
                     /translation="MAPRTIIDKIWDGHEIADLGGGQSLLHIDRVFLHERTGPALLAG
                     LARAGRKPARPELVFGIMDHAVETLPGRLERSRGPGANVFVTGFRDCTKDAGIRLFDL
                     DDPRQGIVHVVSPEQGIALPGCTLVCPDSHTGTVGGIGALAWGIGSTEGEHAVATQTL
                     VLARPGRMRVEFTGRLAEGVGAKDMVLALIGRFGTAGGDGHAIEFTGEAVRSLAVEGR
                     LTLCNMAVEFGAWTGIVAPDDATIAWIAGRPFAPRGGLFDRAAAHWRGLASDDEASWD
                     AFRTFDCSALAPQVTWGTSPEHVLAIDGVVPDPAKASGAARAALERALDYMDLRPGAP
                     ILGTPIDAAFIGSCTNSRLPDLRVAAATVRGRKVAPGVKAIVVPGSTQVKRDAEAEGL
                     DRVFREAGFEWRESGCSLCFFAGIDSFGAARRVITSTNRNFEGRQGPRVRSHLASPAT
                     VVASAIAGRIADPRMSAGS"
     gene            7252..>7795
                     /gene="leuD"
                     /locus_tag="VFV55_06050"
     CDS             7252..>7795
                     /gene="leuD"
                     /locus_tag="VFV55_06050"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX4943896.1"
                     /translation="MEKFTCIEGIAAALYRPNIDTDLIIPSREITSPGREGYGEKLFA
                     PWRYLGPQRCENPDFVLNREPFRAAPILIAGENFGCGSSREMAVWALRQFGIRVIVAP
                     SFGTIFRGNCWRNGVLAVELPGEVVAALARRAETGRLALAVDLAACTVTEPDGTAHSF
                     TMAGREREMMLEGLDDIALTL"
BASE COUNT         1123 a         2613 c         2758 g         1301 t
ORIGIN      
        1 tcgacgcgtt cctccccaag ccgatcgcca tcagggaatt caccgacacg gtcgcctggc
       61 agatcgccag gcggaccggg gggaccccat gaccgcgaag gcgcgcatcc tcgtcgtcga
      121 cgacgtgccc aacaacgtga agctgctcgc ggacctgctc gcggtccgcg gatacgaggt
      181 gctcaccgcg tgcgacggcg acgaggcgct cgagcgcgtg aaggactcca ggccggacct
      241 ggttctcctc gacgtgatga tgccgcggat gaacgggtac gacacctgcc gcgcgatccg
      301 ggcggacccg gcaaccggca tccttcccat cgtgatggtc accgcgctcg acccggccgg
      361 cgagcgcatc aagggcatcg aggcgggcgc ggacgacttt ctcacgaagc ccatcaacgt
      421 ccaggaactc ctcgcgcgcg tgaagtcgct gctgcgtgtc cagtcgctct tccaggaggt
      481 cgcgcggcag aagagcgagc ttgccgaatg gaaccggacc ctcgaggcgc gcgtcgccga
      541 gcaggtggcc caggtcgagc ggctctcgcg gctgcgccgg ttcctgtcgc ccaaggtggc
      601 ggacctcatc gtcgccggcc ggctcgacga cccgctcgcc acccgccggc gggaggtggt
      661 gatcgtgttc gtcgacctgc gcggcttcac cgcgttcacc gagacggcgg accccgagga
      721 agtcttcgca gtcctgcgcg cgtaccacgc cgggatcggc cgcctcatcg cgcggcacga
      781 gggcacgatc gagcacttcg cgggcgacgg cgtgatgatc gtattcaacg accccgcccc
      841 cctgccggat ccggccttct cggcggtgag ctttgcgatc gatgcacgcg acgccgtggc
      901 ggaactcgcc gagggctgga agaagcgcgg ctacggcctg ggctgcggcg tgggcatcgc
      961 gcagggctac gcgaccatcg gcaccatcgg ctacgaggga cgctacgatt acggcaccat
     1021 cggcaccgtg gccaacctcg cggcgcgtct ctgcggcgag gcgaaggcag gggagatcct
     1081 cgtggcgagg aaggtgcatg gcctggtgga ggaccgcgtg ctcgccgggc cgtcccgcga
     1141 cctcgcgctc agggggttcc acgagccggt cgccgcctgc agcgtcatcg ggcgaaagga
     1201 ggggacgtcg tgacgacgag gaatgcgcgg gccgaagccc cgttcatcct gacacccgag
     1261 gagctcgcgg cgatatcgtc gaacgcgccg gcgcggcact accccccgaa gaccatcatc
     1321 gtgaccgagg gggaccaggc cggcgccctc ttcgtgatcc tcgaaggatc gtgcaaggcg
     1381 tacgtcagcg gcgaggatgg ccgcgaagcc gtattgtcag tcatgggccc cggggagtac
     1441 ttcggcgaga tcgcgctgga cgagggcccg cgatcggcct cggtgatgac gctcgagcct
     1501 tcgcgcctgc tcgtcgtgcc gcaggagggc ttccaggcat tcctcgccgg caatcccgat
     1561 ttcgcggcgc acttcatccg caagctcatc ggccgcatcc gggagctcac ccggatcgtc
     1621 ggcaacctgt ccctgctcga cgtgtacgga cgcgtggcgc ggctgctcct cgattccgcg
     1681 cgggaggagg aaggcctgct cgtgattccg gagcgcctca cgcaaatcga cattgcaagc
     1741 cgcgtcggct gctcgcgcga gatggtgagc cgcatcttca aggacctcgt ccaggggggc
     1801 tacgtctcgc tcgaaaagga gcgaatcctg attcaccgcc ggcctccggc ccggtggtga
     1861 gcgttcacca aggcgcccgg gatcgcctgc gttgcgcgcc cgcggccggc ggcggatcat
     1921 cgatgagacg atcgcgaccg agaggaagac catgaaagcg cttttcgacc gctacctgca
     1981 gaagaagatc gggctcaact accacacggc gtaccacatc gacgaagcca cgctcacgct
     2041 cgcagccgat acctacttcg ccgccctcgg cgaaagggat ggcgcgaccc actactaccc
     2101 gtattccagc gtgatccacg tgatggagaa ggcgggcggc gtcgaggtcg gcggcctgtt
     2161 cacgcacaag cacgcctggc cgctcgtggt gaaggtcggc cacgtggtcg acgtgggccc
     2221 gatggtctag cggcccgcgc ggccctcagt cgagctcggg cagcttcgcg aggagcttcg
     2281 cgtaacgcgc gttgtcgaac gcacgccccg cggccagcat ggcctgcatc tcgttccggg
     2341 cgacggcggc gatgagctgc atcgcctcgg cgttggtgct tccggcggcc accaggcgat
     2401 cgagcgttgc cttggtctcg ggcggatccc cggtgtcgag ctggttcacg acgacttcca
     2461 tgaaggcgtc ccggacggtc ctgtcgttca tgagcctctc ccctgcggat cggacacctg
     2521 catattgcgc cgtgccccgg gcttcgtcat gcccgggacc gcgagccgtt cgacgcgttc
     2581 gacggcgcgc gcgagttccg cccggcgagg atcggtggcg ccgagctgat cgagcagtga
     2641 acgccagtgg cgcgccgcat tcgcgtgatg tccctgctcg atggcgaggc tcccggccat
     2701 gtcgagcgca cgcgggtgcg agccgtcgag gtcgagggcg cgcgcgatca accgcgcggg
     2761 cttgccttcg aggcgcccgc cctgcgccag ccccgcggca tccgcgtagt cgcaccagat
     2821 gccggggtcg agcgccacct tcggatccgc gaccgcattt tcgaacgcgg cttccgattc
     2881 cgcgaagcgg tcgagcgcca gttcgacccg gccgagcaac gcccaggccc gcgcatcgcg
     2941 cgggttgtcc gcgagatgcc tcgcgagctg gtcgcgaaag gcgggggcct gcgcggcggt
     3001 gaggggaccc gcgagggtcc cgaaggcgcg cgagctctcg atggcttgcg gtttgccgaa
     3061 cccggcgtac agggcgaggg ccgcgaccgg caacgcgacg gcgactccga gcgccgcgag
     3121 ccatggccgg cgccggtcca cgggcgccgt cctgtcgtcc aggcagatgc cggtcacgcg
     3181 tcgggcccta cttcggcgcg gcagccgcgg gagcggaggc cggcacggcc gccggggcgg
     3241 ctgcgatggt gcgcttcttc cccatggcgt cctcgagggt cttgatggcc tcctgcgaga
     3301 tcaccatgcc cttgttgatc gactcgtcgg cgagcttcgg gttgtggaag tacgagccgt
     3361 tggaggcggt ccagaactcc cagtgcacgt gcgcctcgta gtgcttctcg cgcaccacgt
     3421 tcagcgtcgc ctcatccacg ccgaggttct tcgcctcctc gaacttgtcg atcatgcgcg
     3481 tgagccagta ctcggccttg cgcagcttgc cctggtagcg gttcctgagg gaatcgatcg
     3541 tgtagaccgc ctgctcgcgg ttccacgtct tgtggcagcc caggcacgct tccttcacgt
     3601 attccttcgg gctcgtctgc cagtgcgagg tgaatgcctt gccggtcttc gcgtccttca
     3661 tcttcggcat gtggcaggtg tggcacatcg cgccttccct gtcgtgcacc gagccgtagt
     3721 acacctcggt gtccgggtgc tgcgccttcc agagcagcgc gcccgtgatg gcgtgcttga
     3781 agtcgcggaa cttgaggtcg cggtagtgct gcgcgaggtc gttcacgttc ttcagcggga
     3841 agtggttggt gcgctggtcg gccatggtga ccggctgccc cgtcgcggag tccgtgcccg
     3901 ggttgcagtt gtactcgacg tggcactggc cgcactgcag cttggagtcg tacttgtcga
     3961 ggatcgcgat ctttcgcgtg tagccgcgct ggccgagctc cttcacgtcc accttcccgg
     4021 ccttcgcatc cttgtgccag agcgtgtcct tctcgggccg cgtgagcgcc tggatgaggc
     4081 cgtcgcggat gatgcgcggc tgtgcgccgt gcgggtcgtg gcagaagatg cagctcagcg
     4141 aatggcgcgt gtccttcacg aactcgttca ctttcgacag ccggctccac ttcgccttcg
     4201 ggtcggggtc tcccatgtag gcccagtcca ggatgtggtc ggcggtcttg caggacatgc
     4261 acaccgggtt cgccgccgcg gcggtgccgg ggcggaaggc cttgtgcggt tcgcccggat
     4321 acttgtcgac cagcacgtcc cagatgttga agttgccgcc ggcgcgggcg aggtcgcccc
     4381 agccgtactt gttctcgaag cggccgccga aggcgcggtc gacgacgagc tggtcgtaga
     4441 gcgcgaaggc atgcgagcgc ggcatgttgt gttccttcgt gaagccgtgc gggcccatga
     4501 gcttttccca cgccgggttg ggcgagacgc cggtgagctg gctcttctcg ttcctcgccg
     4561 tcttcgcgaa gttcatcttg tacatcgtct cgaactggtt cctgtggcac gagccgcact
     4621 tggccgggtc ggtgatcgtg accgggcgcg tgctcttctt cttcaggtgc gcatcggtcc
     4681 cggtgtggca ctcggtgcag gccacgccct tgtgcttgcc gggatcgtgg aactccttga
     4741 gttcctcgtg gcaatcgagg cactccctgt tggccgcggg agccttgccg gtggcgacct
     4801 ttgcgggcgc cgcgggcttg gcggcgggtt tttcggcggc tgcgggagca gcctgggctg
     4861 aacacaacgc gaatacggcg agcaggccaa aggcccaccg cacgatggcg cgggatctga
     4921 tgctgttcat ggggggttcc tggggtcgga atgcggggcg acgctccaaa aaccctagca
     4981 agtgcagggt ttacaccctt gatgaagatc aacggtgctg cgatgcgtcg aaccggaagt
     5041 cgaacgaccg ggtgactgtc acggcacccg tggcgtctcg cgtgcgctcc tcgaagcgtg
     5101 cggcggcgtg gcccgcgcgc agcacggtgg aagctcgcgt gccgtagtcg gcgccaacga
     5161 tgagcacggg cgaaagcatg cgctcgcgct cgaggcccac gccggtgtcg gggagttgcg
     5221 aatcgggagc gggcgtggtg tcggatagca tggcgaagag gggctcgtcg ccgtcatcga
     5281 ccgcttcccg catcgcctgg cgcgccttcg cgaccttggg ccacggctcg tcgaggagat
     5341 ggttggaaag cgcgtgcacg cccggttcca cgtcgcgcac ctcgccctcg cggctgccga
     5401 agtaggcgag cgagctgccg tcgctcacga gcaggttgaa gccgttgtat tgcgcggcgc
     5461 ggctcgcgag gccggcgagg taccgcatgg ggctgtcggt tcccagcaaa aactccgcaa
     5521 ccagcgcacc gcgggaaagc gcggtggagc gccgttccga gggatcgcgg aagttggtca
     5581 ccgccgcgaa gcgcccggcg cgcgttatgc ccatccacgt gccaccggcc tgcaggtcgc
     5641 gccccgcgag cagccgcggg tgatccttcc accagtccgc cggcgtggcc ggccggtcgc
     5701 gccactcgtc gcggtttgcc gcgacgacga gcgggaactc cgggtgggcc tgccaggcga
     5761 tggcgatgag gcacatggga caatggcaat tagggacatt cccaatgggg actgtcccta
     5821 ttttgcggca aacatggcgc cacgcacgat catcgacaag atctgggacg ggcacgagat
     5881 cgcggacctc ggcggcgggc agtcgctgct gcacatcgat cgcgtcttcc tgcacgagcg
     5941 cacgggaccg gcgctcctcg cgggactcgc ccgggcgggc cggaagccgg cgcgtccgga
     6001 actcgtgttc gggatcatgg accacgccgt cgagaccctt cccggtcgtc tcgagcgctc
     6061 gcgcgggccc ggcgcgaacg tcttcgtcac gggcttccgc gactgcacga aggacgcggg
     6121 gatccgcctt ttcgatctcg acgatccgcg gcagggcatc gtgcacgtgg tatcgcccga
     6181 gcagggaatc gcgttgccgg gctgcaccct cgtctgcccg gacagtcaca ccggcaccgt
     6241 gggcggcatc ggcgcgctcg cgtggggcat cggctcgacc gagggcgagc atgcggtggc
     6301 cacgcagacg ctggttctcg cgcgacccgg gcgcatgcgc gtcgagttca ccggacgcct
     6361 tgccgagggt gtcggcgcca aggacatggt tctggcgctc atcggacgct tcggtacggc
     6421 cggtggcgac gggcatgcca tcgagttcac cggcgaggcg gtgcgctcac ttgcggtcga
     6481 gggccggctc acgctgtgca acatggcggt cgagttcggc gcctggacgg gcatcgtggc
     6541 accggacgac gcgacgatcg catggatcgc cgggcggccg tttgcgccca ggggcgggct
     6601 cttcgatcgc gccgcggcgc actggcgcgg ccttgcctcc gacgatgaag cgagctggga
     6661 cgcgttccgc accttcgact gttcggcgct ggctccgcag gtgacctggg gcacgagccc
     6721 ggagcacgtg ctcgcaatcg acggcgtcgt tccggatccg gcgaaggcat cgggggccgc
     6781 gcgcgcggcg ctcgaacggg cgctcgacta catggacctg aggcccggcg cgcccattct
     6841 cggcacgccc atcgacgcgg ccttcatcgg ctcgtgcacc aacagccgcc tgcccgactt
     6901 gcgtgtggca gcggccaccg tgcgcggacg caaggtggcg cccggcgtga aggccatcgt
     6961 cgtgccgggc tccacgcagg tgaagcgcga cgccgaagcc gaggggctcg accgcgtctt
     7021 ccgcgaggcc ggcttcgagt ggcgcgagag cggctgctcg ctttgcttct ttgccggcat
     7081 cgattcgttc ggcgcggcgc ggcgcgtgat caccagcacc aaccgcaact tcgaagggcg
     7141 gcagggtccc agggtgcgtt cgcaccttgc cagcccggca acggtcgtgg cctcggcaat
     7201 cgcggggcgc atcgccgacc cgcgcatgtc tgcgggaagc tgaggcgcgc catggagaag
     7261 ttcacctgca tcgagggcat cgcggctgca ctctaccgcc cgaacatcga caccgatctc
     7321 atcatcccgt cgcgcgagat cacgtcgccg gggcgcgagg gctatggcga gaagctgttc
     7381 gcgccgtggc gctatctcgg gccgcagcgg tgtgagaacc cggacttcgt gctcaaccgc
     7441 gagccgttcc gcgcggcacc gatcctcatc gccggcgaga acttcggctg cggttcctcg
     7501 cgcgagatgg cggtgtgggc gctgcggcag ttcggcatcc gcgtgatcgt ggccccctcg
     7561 ttcggcacca ttttccgcgg caactgctgg cgcaacggcg tgctggcggt cgagcttccc
     7621 ggggaggtgg tggccgcgct cgcgcggcgc gccgaaacgg ggcgccttgc gctcgccgtc
     7681 gatctcgccg cgtgcacggt gacggagccc gacgggacgg cgcattcgtt cacgatggcg
     7741 ggacgcgagc gcgagatgat gctcgagggc ctggacgaca tcgccctcac gctca
//
LOCUS       DASUEV010000186         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1297378, whole genome shotgun sequence.
ACCESSION   DASUEV010000186 DASUEV010000000
VERSION     DASUEV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297378"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFV55_06055"
     CDS             complement(<1..966)
                     /locus_tag="VFV55_06055"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX4943897.1"
                     /translation="MLSDIEIAQKAKMKPIGEIAAGLGIPDSAIEPFGHTKAKISLDY
                     IETLKGRPDGKLILVTAISPTPAGEGKTTTTVGLGDALNRIGKKATICLREPSLGPVF
                     GMKGGAAGGGYAQVVPMEDINLHFTGDFNAIALANNLLAAMIDNHINHGNDLGFDVRR
                     IAWKRVMDMNDRALREITIALGGPANGFPREDGFDIVVASEVMAIFCLSTSIKDLKER
                     LGNIVCGYTRDQKPILARELKTHGAMAALLKGALAPNLVQTLEHSPAFIHGGPFANIA
                     HGCNSVLATQTALKLSDYVVTEAGFGADLGAEKFVDIKCRKAGLKP"
     gene            1132..1605
                     /locus_tag="VFV55_06060"
     CDS             1132..1605
                     /locus_tag="VFV55_06060"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4943898.1"
                     /translation="MHPHEALVREFYAAFARRDAEAMARSYHDEVFFSDPVFPKLRGE
                     EARDMWRMLVARASDLEIVLDEASADGEGGRARWTARYTFTRTGRKVVNRIEAMFAFR
                     DGKIVRHFDRFSFWRWSAEALGPLGRVLGWSAPVKWMVRRQAASQLDRYRERNAA"
     gene            complement(1614..2486)
                     /locus_tag="VFV55_06065"
     CDS             complement(1614..2486)
                     /locus_tag="VFV55_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX4943899.1"
                     /translation="MTNLQLFAACVAIWGSTWIAITYQLGAVAPEASVFYRFLLASAM
                     LFAYCLVRGLPLRFSRREHLWIALQGVLMFSVSYVCVDYAEQHVVSGLVAVGYSASPL
                     LNMVGLRVFFGAPMTARVGAGAVLGIVGITLVFWPEFAHLRDDGSVALGALFTAGAVV
                     LSALSNMAAHRNHDAGLPVWQNMAWGMLYGAVFTLALTLSLGQPLGFEATPAYVLSLL
                     YLTVFGSILAFGGFLTLMGRVGAARAAYVGVMVPIVALVISALFEGYRWEWLALVGVG
                     VSVAGNVIILRRPA"
     gene            complement(2483..>2683)
                     /locus_tag="VFV55_06070"
     CDS             complement(2483..>2683)
                     /locus_tag="VFV55_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX4943900.1"
                     /translation="LNDSQVPYWEAAKYVAKLRACRTDSNVLLLKTILEAGHGGASGR
                     YDALKELSFTYTFILGQVGLAH"
BASE COUNT          459 a          883 c          911 g          430 t
ORIGIN      
        1 gggcttcagc cccgccttgc ggcacttgat gtcgacgaac ttctcggccc ccaggtcggc
       61 gccgaagccg gcttccgtga cgacgtagtc gctgagcttg agcgcggtct gcgtggcgag
      121 caccgagttg cagccgtgcg cgatgttggc gaacgggccg ccgtggatga acgcgggcga
      181 atgctccagc gtctgcacga ggttgggggc gagcgcaccc ttgagcagcg cagccatggc
      241 gccgtgggtc ttgagttccc gggcgaggat cggcttctgg tcccttgtgt agccgcagac
      301 gatgttgccg aggcgctcct tcaggtcctt gatcgacgtg gacaggcaga agatcgccat
      361 gacctcggaa gccaccacga tgtcgaagcc gtcctcccgc gggaagccgt tggccgggcc
      421 gcccagcgcg atggtgatct cgcgcagcgc acggtcgttc atgtccatca cgcgcttcca
      481 cgcaattcgg cgcacgtcga agcccaggtc gttgccgtgg ttgatgtggt tgtcgatcat
      541 cgcggccagc aggttgttgg cgagcgcgat ggcattgaag tcgccggtga agtggaggtt
      601 gatgtcctcc atgggcacga cctgcgcgta tccgccgccc gctgccccgc ccttcatgcc
      661 gaacacgggg cccagcgaag gctcgcgaag gcagatggtc gccttcttgc cgatgcggtt
      721 gagcgcgtct cccaggccga ccgtggtggt ggtcttgcct tcgcccgccg gggtggggct
      781 gatggcggtc acgaggatca gcttgccgtc gggccggccc ttcagcgtct cgatgtagtc
      841 gagcgaaatc ttggccttgg tgtggccgaa cggctcgatc gccgagtcgg ggatgcctag
      901 cccggcagcg atctcgccga tgggcttcat cttcgctttc tgggcgatct cgatgtcgga
      961 cagcatgact cttctccttg ttggaccttg cgtgcgggaa acgggctgcc gccccatgcg
     1021 gaaaagggga gtcggcgccc tacctgggtt gcccggagta taggaacgcc ccaaaatgcg
     1081 gagccttgac tcgggtcaag gcttgatcgc caacaagaaa ggtgcgttgc catgcatccc
     1141 catgaagccc tcgtccgcga gttctacgcc gccttcgcgc gccgcgacgc ggaggcgatg
     1201 gcgcgtagct atcacgacga ggtgttcttc tccgatcccg tcttcccgaa gctgcgcggc
     1261 gaggaagcac gcgacatgtg gcgcatgctc gttgcccgcg cgtccgacct ggagatcgtg
     1321 ctcgacgagg cgagcgccga tggcgagggc gggagggcgc gctggaccgc gcgctacacc
     1381 ttcaccagga ccgggcggaa ggtggtgaac cgcatcgagg cgatgttcgc cttccgcgac
     1441 gggaagatcg tgcgtcattt cgaccgcttt tccttctggc gctggtcggc cgaggcgctg
     1501 ggccccctgg gcagggtgct cggctggtcc gctccggtga aatggatggt gcgcaggcag
     1561 gcggccagtc agctcgaccg ctaccgcgag cgcaatgccg cctgagcgcc cggtcaggcc
     1621 gggcggcgca ggatgatcac gttgcccgcg acggaaacgc ccacccccac gagcgccagc
     1681 cattcccagc ggtagccctc gaacagtgcc gagatgacca gcgccacgat cggcaccatg
     1741 acgcccacgt acgcggcccg cgccgcgccg acgcggccca tcagggtgag gaatccgccg
     1801 aaggcgagga tcgagccgaa aaccgtcagg tagaggagcg aaagcacgta ggcgggcgtc
     1861 gcctcgaagc caagcggctg ccccagcgac agcgtgaggg ccagcgtgaa gaccgcaccg
     1921 tacagcatcc cccaggccat gttctgccag acgggaaggc ccgcgtcgtg gttgcggtgc
     1981 gccgccatgt tggatagcgc cgagaggacg accgctcctg ccgtgaacag tgcgcccagc
     2041 gccacgctcc cgtcgtcgcg caagtgcgcg aactccggcc agaagaccag cgtgatgccc
     2101 acgatcccga gaaccgcgcc tgcgcccacc cgcgccgtca tcggtgcccc gaagaacacc
     2161 cgcaatccca ccatgttgag gagcgggctc gcggaatagc caacggctac gagcccggaa
     2221 acgacgtgct gctccgcgta atcgacgcac acgtagctca cgctgaacat gagcacgccc
     2281 tggagcgcaa tccacaggtg ctcgcgccgc gagaaccgca ggggcagtcc gcgaacgagg
     2341 caatacgcga agagcatcgc cgaggcgagc aggaagcggt agaacacgct cgcctcgggc
     2401 gcgaccgccc ccagctgata ggtgatcgcg atccaggtcg agccccagat cgccacgcac
     2461 gcggcgaaga gctgcaggtt ggtcaatgcg ccagcccgac ctggcccagg atgaacgtgt
     2521 aggtgaacga aagttccttc agcgcgtcgt agcggcccga ggccccgccg tggccggcct
     2581 cgaggatcgt cttgaggagc aacacgttcg aatccgtcct gcaggcgcgc agcttcgcca
     2641 cgtacttggc cgcctcccag tagggaacct ggctgtcgtt cag
//
LOCUS       DASUEV010000187         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_59645, whole genome shotgun sequence.
ACCESSION   DASUEV010000187 DASUEV010000000
VERSION     DASUEV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59645"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..838)
                     /locus_tag="VFV55_06075"
     CDS             complement(74..838)
                     /locus_tag="VFV55_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4943901.1"
                     /translation="MQIEKHVFLVAGGGSGLGAATAQRLVSEGANVVIADVNDAGGWV
                     ARQFGDQGRFIRTDVTDEAQVKAAVDLCVSAFGAIHGVVNCAGVAPGERVVGKTGPHS
                     LASFERTVKINLVGTFNVIRLAAATMSTQPAFASGERGVIVNTSSVASFEGQIGQAAY
                     AASKAGVNGMTLPIARELARFGIRVVTIAPGIFDTPMLQGMSEELRASLGAQVPFPPR
                     LGRPDEYAALVAHIIENEVLNGEVIRLDGAIRMGAK"
     gene            948..1871
                     /locus_tag="VFV55_06080"
     CDS             948..1871
                     /locus_tag="VFV55_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4943902.1"
                     /translation="MSLLPDSVRVAVRGWLNCNQVLLRSSGGHVLVDSGYQACADETL
                     ARLRAPEMLGAEALASLVNTHCHSDHIGGNAAIARAYGCPITIPRDEVPTIDDWSIQE
                     RWNAYVDQYAEPFAYQSTIAAGEAFRAADREWQAWAAPGHDMEALMFFDPGERILISG
                     DALWENGLGFVWAHEPPNPFVEAALSALDTIERLAPRVVIPGHGEPFADVAAALARAR
                     SRLGAFADDPRRTARHMMKVMFVFSMLHRGGIALAEADAYALRVPCYRDLDERFLGEG
                     MDGLGTRLVEDLVRAGAMRIEDGWARPTMAR"
BASE COUNT          369 a          818 c          858 g          405 t
ORIGIN      
        1 cctcgctgtt gccccctgcg ggcgttattc cgctgggccg gacgcgctgc ccgttgctga
       61 aggggaactg gacctacttg gcgcccatgc gaatggcgcc gtcgaggcgg atgacttccc
      121 cgttcagcac ttcgttttcg atgatatgtg cgacgagtgc cgcgtattcg tccgggcgcc
      181 ccaggcgggg cgggaagggg acctgcgcgc cgagggaggc acgaagctcc tcgctcatgc
      241 cctggagcat cggggtgtcg aagatgccgg gtgcgatggt gaccacgcgg atgccgaaac
      301 gggcgagttc ccgggcgatg gggagcgtca tgccgttcac gccggccttg gaggcggcgt
      361 aggcggcctg cccgatctgg ccctcgaagg aggcgaccga ggacgtgttc acgatcacgc
      421 cgcgctcgcc cgaggcgaac gccggctgcg tgctcatcgt cgccgcggcg aggcggatca
      481 cgttgaaagt gccgaccagg ttgatcttca ccgtgcgctc gaaggaggcg agcgagtgcg
      541 ggccggtctt gccgacgacg cgctcgccgg gcgccacgcc cgcgcaattg acgacgccgt
      601 ggatcgcgcc gaacgccgag acacaaaggt cgaccgccgc cttgacctgg gcctcgtcgg
      661 tgacatcggt gcggatgaag cggccctggt cgccgaactg ccgggcgacc cagccgccgg
      721 cgtcgttgac gtcggcgatg accacgttgg cgccctcgga gacgagccgt tgggcggttg
      781 ccgcgcccag gccggagccg ccgccggcga cgaggaagac gtgtttttct atttgcatgg
      841 tcgggaggga cgaaatgaag ggaggcaggt gcaaattata gtccctcggg ccgacgcgcc
      901 ggccgcgtta agatggcggc cttcccgaca agcctcgccg gatccccgtg tccctgcttc
      961 ccgacagcgt gcgcgtcgcc gtgcgtggat ggctgaactg caaccaggtg ctgctgcgct
     1021 cgtccggcgg ccatgtcctc gtcgactcgg gctaccaggc ctgcgcggac gagacgctgg
     1081 cgcgcctgcg cgccccggaa atgctcggcg cggaagcgct ggcctcgctc gtgaacaccc
     1141 attgccattc cgatcacatc ggcggcaacg ccgccatcgc gcgcgcctat ggatgcccga
     1201 tcacgatccc ccgcgacgag gttccgacca tcgacgactg gtcgatccag gaacgctgga
     1261 acgcgtatgt cgaccagtac gccgagccgt tcgcctacca gtcgacgatt gccgccggcg
     1321 aggcgtttcg cgccgcggac cgggagtggc aagcctgggc ggcgccgggc cacgacatgg
     1381 aagcgctcat gttcttcgat cccggcgagc gcatcctcat ttccggggac gcgttgtggg
     1441 agaacgggct gggcttcgtc tgggcccatg agccgcccaa tccgttcgtc gaggcggcgc
     1501 tctcggcgct ggacaccatc gagcggcttg ccccgcgcgt cgtgatcccg ggccacgggg
     1561 agccattcgc ggacgtggcc gcggcgctgg cgcgcgcccg gtcgaggctg ggcgcattcg
     1621 ccgacgatcc ccggcgcacg gcgcgccaca tgatgaaagt gatgttcgtc ttctccatgc
     1681 tgcaccgcgg ggggatcgcc ttggcggagg cggacgccta cgcgctgcgc gtgccgtgct
     1741 accgcgacct cgacgagcgt ttcctgggcg agggcatgga cgggctgggc acgcggctgg
     1801 tcgaggacct cgtccgggcc ggggcgatgc gtatagagga cggctgggcg cgaccgacca
     1861 tggcgcggtg aggcggtggt ggtggaagtc gatcgcaatc ctgggcacgc aagcttgacc
     1921 gtcggatggg tgcccggaaa gtcgaggcca ggcagaacaa gccaggcctc gactttccac
     1981 ccatctagag acgagggcgc tgacgctttc tcgtccgcga cggtcgcgtg ccaaggattg
     2041 cgatcgactt ccaccgccgc cgcctcccac gctccgtgga tacagcagcg agtgaggcaa
     2101 cgccggacca cttctcgtgc gctgccggac ggaccgcgtc ccgtccgctg ccgaatgcgc
     2161 cacgctgcct cttcgtgttg gccggggatg gggcaagtta atcggcgtcc ttggcacgcg
     2221 accgtcggga tgggagagcg cagcgccccc atctctcaat gggtaaaaag tcggggcctg
     2281 gcttgttctg cctggccccg actttttggg acccattcga cggtcatgct tgcgtgccca
     2341 ggacgccgat agacttgccc catcgccagc ccgattccaa attcgccggt cacgcttgcg
     2401 tgtccaggac gccgatagac ttgccccatc cccggccctc atcccaattc
//
LOCUS       DASUEV010000188         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1429456, whole genome shotgun sequence.
ACCESSION   DASUEV010000188 DASUEV010000000
VERSION     DASUEV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429456"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2050
                     /gene="fusA"
                     /locus_tag="VFV55_06085"
     CDS             <1..2050
                     /gene="fusA"
                     /locus_tag="VFV55_06085"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX4943903.1"
                     /translation="IVPKYTESIHTLALVGHGSVGKTTLAEALLWKSGAIATPGSVEK
                     GTTVCDFDPMEKAYGHSLGSSLVNFAYEDMRIHLIDTPGMPDFAGQSIAALAAVETAV
                     VVINAQNGIELNTNRMMKWAKTRGLCRMIVVNKIDADNVDLPGLLRKIQDYFGKECLP
                     INLPAHDRKDVVDCFFNPDGDADFVGVKAAHSAIVDQVVEVDEDLMALYLEQGEDLKP
                     EQLHAPFEKALREGHLVPVCFTSARTGAGIPQFLEILAKLAPNATEGNPPPFVKSETT
                     EGEEFHAVPDRTKHVLAHVFKIVMDPYVGKVAYFRVHQGTITKDSQLFVGDSKRPFKV
                     GHLYSVQGKEYIEVNELFPGDLGAIAKVDDIDFNAVLHDSHEEDNIRLRSLDFPTPMH
                     SIAVETKKKGDEQRLFDVLHKLEVEDPCLWIERHPTTHETVVRGLGELHMRVKLEKML
                     TQYKLEVDTKPPKIPYRETITKKAEGHCRHKKQTGGAGQFGEVFLKVEPLSRGSGFEF
                     VDQVKGGVIPTVFIPAVEKGVKMALDMGVIAGYPVEDIRVIVYDGKSHPVDSKEIAFV
                     TAGKKAVIDAILKAGAIILEPIVNITITAPDRFVGDMTSDISSRRGQITGTESSGGDL
                     MSISGQVPLSEVAEYQSRLRSVTGGQGSYQIEYSHYSMVPPQTQVALTSEFKLAREED
                     "
     gene            2302..2859
                     /locus_tag="VFV55_06090"
     CDS             2302..2859
                     /locus_tag="VFV55_06090"
                     /inference="COORDINATES: protein motif:HMM:NF022645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4526/YPO1902 family pathogenicity island
                     replication protein"
                     /protein_id="HEX4943904.1"
                     /translation="MIEIRDQQLRLLVLAHLIRELQRSAEENRPAPPGLSGAQFAELR
                     TLSTDDLVRLSEMQEPRVAVQIDAGSFEHGLRQVGYIGKRSKQIEHFIRHGATSAMLT
                     RLFRISSTDVTLKRRLFAGTHEKLRRPAMPPPAVREAIQKRWWEIRKGKEREPLRPEE
                     YEALHADYPTLTYATLWAVVHEFGD"
     gene            complement(2968..>3083)
                     /locus_tag="VFV55_06095"
     CDS             complement(2968..>3083)
                     /locus_tag="VFV55_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX4943905.1"
                     /translation="RALWLEGREEHGPYQSPMEDGMVHFHEFIRREIAQDL"
BASE COUNT          585 a         1027 c          992 g          479 t
ORIGIN      
        1 catcgtgccg aagtacaccg aatccattca tacccttgcg ctggtcggtc acggcagcgt
       61 cggcaagacc acgctcgccg aggccctgct gtggaagtcc ggcgccatcg ccaccccggg
      121 cagcgtcgag aagggaacga cggtctgcga cttcgacccc atggagaagg cctacggcca
      181 ctcgctcggg tcctccctcg tgaatttcgc ctacgaggac atgcgcatcc acctcatcga
      241 cacgcccggc atgcccgact tcgccggcca gtcgatcgcg gcgctggcgg ccgtggaaac
      301 cgcggtcgtc gtcatcaacg cgcagaacgg catcgagctc aacaccaacc gcatgatgaa
      361 atgggcgaag acgcggggcc tgtgccgcat gatcgtggtg aacaagatcg acgcggacaa
      421 cgtggacctg ccggggctcc tgcggaagat ccaggactac ttcggcaagg aatgcctgcc
      481 catcaacctg ccggcccacg accgcaagga cgtggtggac tgcttcttca atcccgacgg
      541 cgatgccgac ttcgtcggcg tgaaggcggc acactcggcc atcgtcgacc aggtggtcga
      601 ggtggacgag gacctgatgg ccctctacct ggaacagggc gaggacctga agcccgagca
      661 gctgcacgcg cccttcgaga aggcgctgcg cgagggccat ctcgtgcccg tgtgcttcac
      721 gtcggcaagg accggcgcgg gaatcccgca gttcctcgag atcctggcga agctcgcgcc
      781 caacgcgacc gagggaaatc cgccgccctt cgtgaaaagc gaaacgaccg agggcgagga
      841 attccatgcc gtgcccgatc gcaccaagca cgtgctcgcc catgtcttca agatcgtcat
      901 ggacccgtac gtgggcaagg tcgcctattt ccgcgtccac cagggcacga tcacgaagga
      961 ttcccagctc ttcgtgggcg acagcaagcg cccgttcaag gtgggtcacc tctactcggt
     1021 ccagggcaag gaatacatcg aggtgaacga actgttcccc ggcgacctgg gcgccatcgc
     1081 caaggtggac gacatcgact tcaacgccgt cctgcacgat tcccacgagg aggacaacat
     1141 tcgcctgcgc tcgctggact tcccgacgcc gatgcactcc atcgccgtgg agacgaagaa
     1201 gaagggcgac gagcagcgcc tcttcgacgt gctgcacaag ctggaggtcg aggacccctg
     1261 cctgtggatc gagcgccacc ccacgacgca cgagacggtg gtgcgcggcc tgggcgagct
     1321 gcacatgcgc gtgaagctcg agaagatgct gacgcagtac aagctcgagg tggacaccaa
     1381 gccgcccaag atcccctatc gcgagacgat cacgaagaag gccgaaggcc actgccggca
     1441 caagaagcag accggcggcg ccggccagtt cggcgaggtc ttcctcaagg tcgagccgct
     1501 ttcgcgcggc agcggtttcg agttcgtcga ccaggtgaag ggcggcgtga ttcccacggt
     1561 cttcatcccg gccgtggaga agggcgtgaa gatggcgctc gacatgggcg tcatcgcggg
     1621 ctatcccgtc gaggacatcc gcgtcatcgt ctacgacggc aagagccacc ccgtcgactc
     1681 gaaggaaatc gccttcgtga cggcgggcaa gaaggcggtg atcgacgcga tcctgaaggc
     1741 gggcgcgatc atcctcgagc ccatcgtgaa catcaccatc accgcgcccg accggttcgt
     1801 gggcgacatg acgagcgaca tctcctcgcg ccgcgggcag atcaccggca cggaatcctc
     1861 gggcggggac ctcatgtcga tctccggcca ggtgccgctt tcggaagtgg ccgaatacca
     1921 gtcgcggctg cgctcggtga ccggcgggca gggctcctac cagatcgagt acagccacta
     1981 ctcgatggtg ccgccgcaga cgcaggtcgc gctcacctcg gaattcaagc tggcccggga
     2041 agaagactag cgccagggcc gggagtttcg cgggaagccc ggcgatcagg cacatcggcg
     2101 acgaacttcg cgcggtttct cacctcgggt gcgtagtgcg ggcacgtcgg gggcggcacc
     2161 tcgacgtccg tgctgcgctg caccgcgaaa atatcgcgat ttcgcattga cacgccggtc
     2221 ggccgttggc attttcgttc cctcgaccgt accggcgcgc ccgccgcggc cgtaccgtcg
     2281 tcgccaggaa gggggagagc gatgatcgag attcgtgacc agcaactgcg cctgctcgtc
     2341 ctcgcccacc tcatccgcga gctgcagcga agcgccgagg agaaccgccc cgcgccgccc
     2401 ggcctctcgg gggcgcagtt cgccgagctg cgcactctca gcaccgacga cctcgtgcgc
     2461 ctttccgaga tgcaggagcc gcgcgtagcc gtgcagatcg acgcaggctc cttcgagcac
     2521 ggcctgcggc aggtgggcta catcggcaag cgctcgaagc agatcgagca cttcatccgc
     2581 cacggcgcca cctcggcgat gctcaccagg ctcttccgca tctcgtcgac ggacgttacg
     2641 ctcaagcgcc ggctctttgc cgggacgcac gagaagctgc gccgtcccgc catgcccccg
     2701 ccggcggtgc gcgaggccat ccagaagcgc tggtgggaga tccgcaaggg caaggaacgc
     2761 gagcccttgc gcccggagga atacgaggcg ctgcacgcgg actacccgac cctcacctac
     2821 gcgacgctat gggcggtggt gcacgagttc ggggactagg caggaaaagg ggtcaggtta
     2881 ctttgaggaa aaggggtcag gttacttttc tgtcgcattg ccgtcgcaag acggcgggct
     2941 gaaaagtaac ctgacccctt ttcctgccta gaggtcctgc gcgatttcgc gccggatgaa
     3001 ctcgtggaag tggaccatgc cgtcctccat cggggactgg tacggtccgt gctcctcgcg
     3061 cccctcgagc cacagcgcgc ggc
//
LOCUS       DASUEV010000189         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_863213, whole genome shotgun sequence.
ACCESSION   DASUEV010000189 DASUEV010000000
VERSION     DASUEV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863213"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFV55_06100"
     CDS             complement(<1..384)
                     /locus_tag="VFV55_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943906.1"
                     /translation="MFRKLWVWQVILLVALFAFWHVATNPELIPPFVWDNPDRAAFFF
                     GEPVKIFKAIADWFASGEIYPHLWVTLEETALAFVIGSVLGLVIGLWLGLTPFASALM
                     DPYIKAMNAMPRVVLAPIFMVWFGLG"
     gene            complement(388..1200)
                     /locus_tag="VFV55_06105"
     CDS             complement(388..1200)
                     /locus_tag="VFV55_06105"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4943907.1"
                     /translation="MTASVSLEKVTCTFASRSGEGSYTAVKDVDLSIADGEFVSVVGP
                     TGCGKSTLLNVAAGLLKPSSGSVKIHGEPISGINRHAGYMFQADALMPWRSALDNVVA
                     GLQFRGEDLPAARAKAEAWLGRVGLAGFGDRYPHQLSGGMRKRVSLAQMLILDPQILL
                     MDEPFSALDVQTRSLMENELLELWSANRKSVIFITHDLEEAISLSDRVVVLSAGPASH
                     PIGEYVIDLPRPRDVSEIRLTPRFIALHDKIWHAMKDEVLKGYAQTRNRMAG"
     gene            complement(1284..>2204)
                     /locus_tag="VFV55_06110"
     CDS             complement(1284..>2204)
                     /locus_tag="VFV55_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4943908.1"
                     /translation="GKNLFYYLPLSVAERKGYFKDEGLEVEIPDFAGGAKALQALVGG
                     SADMVSGAYENTINMAAKKQPIKAVVLQARYSSIVLLLSKDRAAKYKGPADLKGLKVG
                     VTAPGSSTNMFVNNILAKGGLKPTDISVVGVGAGSGAVAAMEKGEIDALSNLDPVITQ
                     LESTGKFVAVADSRTEKGMNEIYGGDYHASVIYITDEYIKKYPNTVQAVVNAIVRADR
                     WIAKATPQEIVDLMPDTYKAGSPSLYKAGLLKNMIGYSEDGQMSMKAAQNVYKVLKQF
                     EPSVIKAGDSIKLDQTFDNTFARKAAARYK"
BASE COUNT          364 a          712 c          725 g          403 t
ORIGIN      
        1 gcccaggccg aaccacacca tgaagatggg cgccagcacc acgcgcggca tcgcgttcat
       61 ggccttgatg taggggtcca tcagcgcgga ggcgaacggc gtgaggccca gccacaggcc
      121 gatcaccagc cccagcaccg agccgatcac gaacgcgagc gcggtctcct ccagcgtcac
      181 ccacaggtgc gggtagatct cgccgctcgc gaaccagtcc gcgatcgcct tgaagatctt
      241 caccggctcg ccgaagaaga acgcggcgcg gtcggggttg tcccacacga agggcgggat
      301 gagctccggg ttcgtggcca cgtgccagaa cgcgaagagg gccacgagga ggatcacctg
      361 ccagacccag agcttcctga acatgggtca gccggccatc cggttgcgcg tctgcgcata
      421 gcccttgagg acctcgtcct tcatcgcgtg ccagatcttg tcgtgcaacg cgatgaagcg
      481 gggcgtgagg cggatctcgg aaacgtcgcg cgggcgcgga aggtcgatca cgtactcgcc
      541 gatggggtgc gaggcggggc cggccgagag caccacgacg cggtcggaga gggagatggc
      601 ctcctccagg tcgtgggtga tgaagatgac gctcttgcgg ttggcgctcc acagctccag
      661 cagctcgttt tccatgaggc tgcgcgtctg cacgtcgagc gcggagaagg gctcgtccat
      721 gaggaggatc tgcgggtcga ggatgagcat ctgcgcgagc gagacgcgct tcctcatgcc
      781 gccggagagc tggtgcggat agcggtcgcc gaatccggcg aggcccacgc gccccagcca
      841 cgcctccgcc ttcgcgcgcg cggccggcaa gtcctcgccg cggaactgca atccggcgac
      901 cacgttgtcg agcgcgctcc tccacggcat cagcgcgtcg gcctggaaca tgtagcccgc
      961 gtggcggttg atgccggaaa tcggctcgcc atggatcttc acagatcccg acgagggctt
     1021 gagcagcccc gccgcgacgt tgagcagcgt cgacttgccg cagcccgtgg gccccaccac
     1081 cgaaacgaat tctccatcgg caatggacag gtcgacgtcc ttcacggccg tgtagctgcc
     1141 ctccccgctc ctggaggcga aggtgcaggt gaccttttcc agcgatacgg atgcggtcat
     1201 cgcaaaggtt gctcggacat gttgaacggc aggcgaagcc tgtccgttcg aacagggttc
     1261 ccgatggtca tgacgggata gcgttacttg tatcttgcgg cggcctttct tgcgaatgta
     1321 ttgtcgaagg tctggtcgag cttgatcgaa tcgccggcct tgatcaccga gggctcgaac
     1381 tgcttgagca ccttgtagac gttctgcgcg gccttcatcg acatctggcc gtcttcggag
     1441 tagccgatca tgttcttgag caggccggcc ttgtacagcg aggggctgcc cgccttgtag
     1501 gtgtccggca tcaggtcgac gatctcctgc ggcgtggcct tcgcgatcca gcggtcggcg
     1561 cgcacgatcg cgttcaccac cgcctgcacc gtgttgggat atttcttgat gtactcgtcg
     1621 gtgatgtaga tcacgctcgc gtggtagtcg ccgccgtaga tctcgttcat gcccttctcg
     1681 gtgcggctgt cggccacggc gacgaacttg ccggtggact cgagctgggt gatgaccggg
     1741 tcgaggttgg acagcgcgtc gatctcgccc ttctccatcg cggccacggc gccggagccc
     1801 gcgcccacgc cgaccaccga gatgtccgtg ggcttgaggc cgcccttggc gaggatgttg
     1861 ttcacgaaca tgttggtcga cgaccccggg gccgtcacgc cgaccttgag gcccttcagg
     1921 tccgccgggc ccttgtactt cgcggccctg tccttggaca gcagcagcac gatggagctg
     1981 tacctggcct gcagcaccac tgccttgatg ggctgcttct tggcggccat gttgatggtg
     2041 ttctcgtagg cgccggagac catgtccgcc gagcccccga ccaaggcctg cagcgccttg
     2101 gcgccgcccg cgaaatcggg aatctcgacc tcgagcccct cgtccttgaa gtagcccttg
     2161 cgctcggcca ccgacagcgg caggtagtag aacaggttct tgcc
//
LOCUS       DASUEV010000190         4632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_382418, whole genome shotgun sequence.
ACCESSION   DASUEV010000190 DASUEV010000000
VERSION     DASUEV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382418"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..895)
                     /locus_tag="VFV55_06115"
     CDS             complement(47..895)
                     /locus_tag="VFV55_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4943909.1"
                     /translation="MDVYARTVQPALAEALGQTIVIENKTGASGMIGTEAVAKSPPDG
                     YTLVVGNIATFAMNVGIYRKMAYDPVKDLTPIMHTVKVNYVLVVNPEVPVKTVAELIA
                     YARANPGKLSYGSSGSGSAQHMAAELFKAQTGVNLTHVPYKGTGALVGDLIAGHLQLA
                     FADQASMMPQVKAGKLRALGVGGLQRSPEYPDIPTIAESGNLSGYEAVAWQGIAGPAG
                     LPPAVVKRLNEAFLKAHESPGMKEKLAAAGLTSVGSSPEEFGRYIRSEIAKWSKVAKD
                     IGATVD"
     gene            1264..1677
                     /locus_tag="VFV55_06120"
     CDS             1264..1677
                     /locus_tag="VFV55_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEX4943910.1"
                     /translation="MVAIPEVARRNVDLGSKQTAAAALRTFFRIAEAWKLSVKEQMAI
                     LGVRSRSTLHSWKGGEAGPLGRDTLERLSYVFGIYKALQVIFSRQEQANGWIRKPNAA
                     PIFGGRSALDRLVAGNVSDLYEVRRYLDAQRGGWN"
     gene            1684..2388
                     /locus_tag="VFV55_06125"
     CDS             1684..2388
                     /locus_tag="VFV55_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX4943911.1"
                     /translation="MARVAWKPSYRLVHSRFPPVGLFDQVADPADLDAVFAIESLTND
                     RLRDEVGELVLVPLEDRISGPGTTPIMAAFTHPNPEGSRFSNGDYGVYYAAASLETAI
                     AETAYHRSRFLARTREPPSEYDMRCYQADIDAELVELRRKKNRRPELYHPDNYGPAQA
                     FGRKRRAEGALGIVYDSVRHSGGQCVAIFKPRAVPRCVQTVHVCFVWDGETIASYYVK
                     SGYADVGPAGGTMSAP"
     gene            complement(2343..2534)
                     /locus_tag="VFV55_06130"
     CDS             complement(2343..2534)
                     /locus_tag="VFV55_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX4943912.1"
                     /translation="MKTSATPMRRLIVGISGASGVIYGIRALQVLKAVPDIETHLVLS
                     AAASRYGALIVPPAGPTSA"
     gene            complement(2567..3538)
                     /locus_tag="VFV55_06135"
     CDS             complement(2567..3538)
                     /locus_tag="VFV55_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4943913.1"
                     /translation="MNRRALLKSAVALALAPRLALAQAFPSKAIRIVNPFSAGGGLDQ
                     LARNLAQKMAESLGQAVVVENRTGAGGNIGADIVAKEKADGYTLLMGSSATHGISPAL
                     YGPRLPFDPVKDFTAISVSVVQKNVLVVNSNLPARNVAELIAYARANPGKLSFGSPGA
                     GTSQHLSGELFKHMAKVDMVHVPYKGSAAAMTDLLGDRITMMFTDIPTAIPHIKTGRL
                     RALGVTSAQPSPALPDVKPIAEQGLPGFDLKAWYGVMAPAKLPPGVAEKLNAEITKAL
                     TAPDMHEKLMAVGQEPIALNLADSDAYVKTEIARWAEVVKISGAKLE"
     gene            complement(3582..4445)
                     /locus_tag="VFV55_06140"
     CDS             complement(3582..4445)
                     /locus_tag="VFV55_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX4943914.1"
                     /translation="MTSPTDFTQRFILENLDIRGQIVRLGPVWRAILSRRDYPVPLRQ
                     LLGEFASIAVLIASGLKHPGRTVLQVMGQGPITMAVADCTHEFALRSMLKPGEVAVGE
                     ALTLHEYFPSGRLVLTVENATTARHFQSIVPLEGDSLADCFERYFDRSEQVPTHLWVQ
                     ATEESVGALILQKLPKADERDPSGWARVQLQAARMAPLCLGSGAGSEGIAELLTSLFP
                     EDDIRLYKPKPVKDGCTRSEEKVVAMLRGLGRTEVEATLAEHGVVTVRDEICNQEYRF
                     AAPDVERIFEG"
BASE COUNT          746 a         1517 c         1552 g          817 t
ORIGIN      
        1 tcgctggcac ccgacccggc ttaccgtcat cgcgcgcacc gccgcctcag tcgaccgtcg
       61 cgccgatatc cttcgcgacc ttggaccact tggcgatctc gctcctgatg tagcggccga
      121 attcctccgg cgagctgccg acggaggtca ggcccgcggc cgcgagcttc tccttcatgc
      181 cgggcgactc gtgcgccttg aggaacgcct cgttcaggcg cttgacgaca gcgggcggaa
      241 ggcctgccgg tcccgcgatg ccctgccacg ccacggcttc gtagccggaa agattgcccg
      301 actccgcgat cgtcggaatg tccgggtact cgggcgatcg ctgcaagccg cccacgccga
      361 gcgcacggag cttgcccgct ttcacctgcg gcatcatgct cgcctggtcc gcgaaggcaa
      421 gctgcaggtg gcccgcgatg agatcgccca cgagcgcgcc cgtgcccttg tacggaacat
      481 gggtgaggtt cacgcccgtc tgcgccttga agagctcggc cgccatgtgc tgcgcgctgc
      541 cgctgccgga ggagccgtag gaaagcttgc ccggattggc cctcgcgtag gcgatgagct
      601 cggccacggt cttcaccggc acctccgggt tcacgaccag cacgtagttc accttcacgg
      661 tgtgcatgat gggcgtgagg tccttcaccg gatcgtaggc catcttcctg tagatgccca
      721 cgttcatcgc gaaggtggcg atgttgccca cgacgagcgt gtaaccgtcc ggtggggact
      781 tggccaccgc ttccgtgccg atcatgccgc tggcccccgt cttgttctcg atgacgatgg
      841 tctggccgag cgcttcggcg agcgcgggct ggacggttcg cgcatagaca tccacggcgc
      901 cgcccggggg gaaggggacg atgaggcgga cgggtttggt cgggaaggtt tgggcaagcg
      961 tcgcggtggc aacggcgagg gcgaggcagg gaagaagccg gcggagcaaa caagatgtgc
     1021 gagtcatgat ccagtgcgtt gtgattgttg cgggaatcct gaccttaccc cagaacgacg
     1081 agactgcgat cctctcccgg cctgcggcag tgacaggcca tcatccccgg cctctccctc
     1141 gaacttcccg tgctgtccga gtatgtacgt agttgaacaa tatcttgcaa tattgtacag
     1201 ttgcatacat ccccatcgtt tcgcctcttc aatggcggcc tctcgatggg accaggaggg
     1261 ggcatggtcg caataccgga agttgccagg cgcaatgtcg atctcggctc gaagcagacc
     1321 gcggcggccg cgctgcggac attctttcgc atcgcggaag cctggaagct gagtgtgaag
     1381 gaacagatgg cgatcctcgg cgttcgctcc cggtccacgc tgcattcgtg gaaaggtggc
     1441 gaagcgggcc cgctgggccg cgacacgctc gagcgccttt cctacgtgtt cggcatctac
     1501 aaggccctgc aggtcatctt ctcgaggcag gaacaggcga acggctggat caggaagccg
     1561 aacgccgctc cgattttcgg aggccgcagt gctctcgatc gcctggtcgc cggcaacgta
     1621 tccgaccttt acgaggtccg ccgctatctg gacgcgcagc gcggcggctg gaactgacca
     1681 cccgtggctc gcgttgcctg gaagccgagc taccggctcg tccacagtcg cttcccgccg
     1741 gtcgggctgt tcgaccaggt ggccgatccc gcggatctcg acgccgtctt cgcgatcgaa
     1801 tcgctgacca acgatcgcct gcgcgacgag gtcggggagc tcgtgctcgt gccgctcgag
     1861 gacaggatct ccgggcccgg gaccacgccc atcatggccg ccttcacgca cccgaacccc
     1921 gagggaagcc gcttctcgaa cggcgactac ggcgtctatt acgcggcggc cagccttgaa
     1981 acggccatcg ccgaaacggc gtaccaccgg tcccgattcc tggcgcggac ccgggagccg
     2041 cccagcgagt acgacatgcg ctgctaccag gcggacatcg atgccgagct tgtcgaactg
     2101 cgccgcaaga agaaccggcg ccccgagctg taccaccccg acaactacgg gcccgcgcag
     2161 gcgttcggcc gcaagcgcag ggccgaaggc gccctgggca tcgtctacga cagcgtccgc
     2221 cactccgggg gacagtgcgt ggcaattttc aagccgcgcg cggttccccg ttgtgtccag
     2281 acggtccatg tctgcttcgt ctgggacggg gagacgatcg cgagctatta cgtgaagagc
     2341 ggctacgcgg acgtgggccc cgccggcggc acgatgagcg cgccatagcg gctggcggcg
     2401 gccgacagga cgaggtgcgt ctcgatgtcg ggcaccgcct tcagtacctg cagcgcgcgg
     2461 atgccgtaga tcactccgga tgccccggag atgccgacaa ttagccttcg catcggggtt
     2521 gcggaagtct tcatggtcct cgccctcctt cctcgcggga cagtgcctac tcgagcttcg
     2581 cgccgctgat cttcaccact tccgcccagc gcgcgatctc ggtcttcacg taggcgtcgc
     2641 tgtcggcaag attcagcgcg atgggctcct gacccacggc catcagcttc tcgtgcatgt
     2701 ccggcgcggt gagcgccttg gtgatttcgg cgttgagctt ctccgccacg cccggcggca
     2761 gcttggccgg ggccatcacg ccgtaccagg ccttgaggtc gaacccgggc aggccctgct
     2821 cggcaatggg cttcacgtcc ggcagtgcgg gcgagggctg ggccgaggtg acgcccagcg
     2881 cgcgaaggcg ccccgtcttg atgtgcggga tggcggtcgg gatatcggtg aacatcatcg
     2941 tgatccggtc gcccagcagg tccgtcatgg cggccgcact ccccttgtag ggtacgtgca
     3001 ccatgtccac cttcgccatg tgcttgaaga gctcgcccga aaggtgctgc gaggtgcctg
     3061 cgcccgggga gccaaaggag agcttgcccg ggttggccct ggcgtacgcg atcagttccg
     3121 ccacgttcct tgcgggcagg ttcgagttca ccaccagcac gttcttctgc accacggaca
     3181 cggagatggc cgtgaaatcc ttcaccggat cgaagggaag gcgcgggccg tagagagcgg
     3241 ggctgatgcc gtgcgtggcg ctggaaccca tgaggagcgt gtagccgtca gccttttcct
     3301 tggcgacgat gtcggcgccg atgttgcctc ccgcgcccgt gcggttttcc acgacgacgg
     3361 cctgcccgag ggattcggcc attttctgcg cgaggttgcg cgcgagctgg tccagcccgc
     3421 ctcccgcgga gaacgggttc acgatgcgga tggccttgga cgggaaggct tgcgcaaggg
     3481 caagccgcgg ggccagcgca agggcgacgg ccgacttgag aagggcgcgt ctgttcatgg
     3541 aaggcgcctc cggatgggtt ctggtttcag cgcgcctcgt tctagccttc gaagatgcgt
     3601 tccacgtcgg gcgcggcgaa gcggtattcc tggttgcaga tctcgtcgcg caccgtcacc
     3661 acgccgtgct cggccagcgt ggcctcgact tcggtgcggc ccagcccgcg gagcatggca
     3721 accacctttt cctcgctgcg cgtgcaaccg tctttcacgg gcttgggctt gtacaggcgg
     3781 atgtcatctt ccgggaagag cgaggttagg agttccgcga tgccctcgct tccggcgccg
     3841 ctcccaaggc agaggggggc catccgggcc gcctgcagct ggacgcgcgc ccagccactc
     3901 gggtcacgtt cgtcggcctt cggaagtttt tgcaggatga gagcacccac gctttcctcg
     3961 gtcgcctgga cccacaggtg ggtgggcacc tgctcgctgc ggtcgaagta gcgctcgaag
     4021 caatcggcga gactgtcgcc ctcgaggggg acgatggact ggaaatggcg ggcggtcgtg
     4081 gcgttttcca cggtgaggac gaggcgaccg gagggaaagt actcgtgcag ggtcaatgcc
     4141 tcgcctacgg ccacctcgcc gggcttcagc atggagcgca gggcgaactc gtgcgtgcaa
     4201 tcggcaaccg ccatcgtgat cggcccctgg cccatcacct gcagcaccgt ccggcccgga
     4261 tgtttcaggc ccgaggcgat gaggacggcg atcgaggcga attcgcccag caactgccgc
     4321 agcggcaccg ggtaatcgcg gcgggagagg atggccctcc acaccgggcc caggcggacg
     4381 atctggccgc ggatgtcgag gttttcgagg atgaagcgct gggtgaagtc ggtgggcgag
     4441 gtcatcgcga aatgataccg ccctgtcata ctggccccgc cgacacgccg cgaggggtgg
     4501 tgccatggac gacgtcgcaa ggctcatcaa ggcggccgat ttcgccgcct tcaagcatcg
     4561 cctgcaacgg cgcaaggacg ctgcggcatc cccctacatc aaccacccgc tcgcgctggc
     4621 acgcgtgctg aa
//
LOCUS       DASUEV010000191         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1551689, whole genome shotgun sequence.
ACCESSION   DASUEV010000191 DASUEV010000000
VERSION     DASUEV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551689"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /gene="aceE"
                     /locus_tag="VFV55_06145"
     CDS             complement(<1..1241)
                     /gene="aceE"
                     /locus_tag="VFV55_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168826.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX4943915.1"
                     /translation="MDRMQDVDPLETREWVDSLEAVLAAEGPARAHFLLECLIDKARR
                     SGVNLPYSANTTYINTIPPHLEEPLPGDMEIENRLRNIVRWNAVAMVLRAGKKDLELG
                     GHIASFASAATLYDVGFNHFWRAPTESHGGDLIYIQGHSSPGVYARAFLEGRLSEAQL
                     DMFRQEVGGTGISSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFLRYLEDRGLAKTA
                     GRKVWAFMGDGEMDEPESMGAIGMAAREGLDNLVFVVNCNLQRLDGPVRGNGKIIQEL
                     ETHFRGAGWNVLKIIWGSYWDPLLARDTHGILLNRMEEAVDGEYQKFKSRDGAYVREH
                     FFGKYPELKAMVASMSDEDIWRLNRGGHDPHKIYAAYHSAVNHRGQPTVVLAKTVKGY
                     GMGAVGEGKNVAHQQKKMPLEV"
     gene            1708..2532
                     /locus_tag="VFV55_06150"
     CDS             1708..2532
                     /locus_tag="VFV55_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX4943916.1"
                     /translation="MNLSQALQSASLSDPGRVRDHNEDCIEARPEIGLYVLADGMGGY
                     NAGEVASGMATSLIADGLAESWVLRDVDRLPREDAMALAERLIREQVARANTAIFTTA
                     QNNPECAGMGTTLVVCLFYDDFLCVAHIGDSRLYRLRGEVMEQVTRDHSLLQEQLDSG
                     LITPEEAKLSQNKNLVTKALGIDPAVEPELHVYETERGDMYLLCSDGLNDMVEDEEIR
                     LTLIALRSNPNLTVQQLVQAANDNGGRDNVSAMLVLVAEPFAVARGWLARFKALFR"
BASE COUNT          441 a          832 c          842 g          428 t
ORIGIN      
        1 acttccagcg gcatcttctt ctgctggtgc gcgacgttct tgccttcgcc caccgcgccc
       61 atgccatagc ccttgaccgt cttggcgagc acgacggtgg gctggccgcg gtggttcacc
      121 gccgaatggt aggccgcgta gatcttgtgc gggtcgtggc cgccgcggtt cagccgccag
      181 atgtcctcgt cggacatgct tgcgaccatg gccttgagct cggggtactt gccgaagaaa
      241 tgctcgcgaa cgtaggctcc gtcgcggctc ttgaatttct ggtactcgcc gtcgaccgcc
      301 tcttccatgc ggttgaggag gattccgtgc gtgtccctgg cgagcagcgg gtcccaatag
      361 gagccccaga tgatcttgag cacgttccag ccggcgccgc ggaagtgcgt ctccagctcc
      421 tggatgatct tgccgttgcc gcgcaccggg ccgtcgagcc gctgcaggtt gcagttcacc
      481 acgaacacca ggttgtcgag cccctcgcgc gccgccatgc cgatggcgcc catggactcg
      541 ggctcgtcca tctcgccatc ccccatgaac gcccagacct tgcggccggc cgtcttggcg
      601 agcccgcggt cctcgaggta gcgcaggaat cgcgcctggt agatcgcctg gatcggcccc
      661 aaccccatcg acacggtcgg gaactgccag aaatcgggca tcagccacgg gtgcggatag
      721 gaggagatcc ccgtgccgcc gacctcctgg cggaacatgt cgagctgcgc ctcgctcagg
      781 cgcccctcga ggaacgcgcg cgcatagacg ccgggcgagg aatggccctg gatgtagatc
      841 aggtcgccgc cgtggctctc cgtcggcgcg cgccagaagt ggttgaagcc cacgtcgtag
      901 agcgtcgccg cggaggcgaa gctggcgatg tgcccgccca gctcgaggtc cttcttcccg
      961 gcccggagca ccattgccac cgcgttccag cgcacgatgt tgcgcagccg gttctcgatc
     1021 tccatgtcgc cgggcagcgg ctcctcgaga tgcggcggga tcgtgttgat gtaggtggta
     1081 ttcgcgctgt aggggaggtt cacgcccgag cggcgtgcct tgtcgatgag gcattcgaga
     1141 aggaaatgcg cgcgcgcggg accctcggcg gcaagcacgg cttccagcga atcgacccac
     1201 tctcgggtct ccagcgggtc gacatcttgc atgcggtcca tggtggcctc gggattcggg
     1261 agttcaggaa ggcggagcgg cgggaaaggg ctccattaga ccgaatcgta taattgctgg
     1321 caacccggct cgcgcacggt cccgacgggg gattcccggc ccttcggcga cccgtcggga
     1381 gtgcgcgccg gcaggccctt tttgcgaaga agccctagac aaatcagtga catcgggccg
     1441 acaatcggga ctgtttctca aaaaaacatt ttaagttact ataaaaacgg gtttttacag
     1501 ttttcgtccg ggaggccgga cgggccttca gccggtgccg gccattgcga cagcgggcac
     1561 ggcgggcagc ccgcgaccag caaccacaag aaccgagaac gcaatggatc atcgggcgca
     1621 ccggaaaggg gaaggccgag cgcgcagcgc gagccgggag gccgcgccag cgctcgtcct
     1681 gcgatcggac cctcggagtg acgggacgtg aacctttcgc aggcgctgca aagcgcgagc
     1741 ctcagcgacc cggggcgggt ccgggaccac aacgaggatt gcatcgaggc gcgcccggaa
     1801 atcgggctct acgtcctcgc cgacgggatg ggcggctaca acgccgggga agtggcgagc
     1861 ggcatggcaa cttcattgat cgccgacggg cttgccgaat cctgggttct gcgggacgtg
     1921 gaccggttgc cccgcgagga cgcgatggcc ctcgccgagc gcctgatccg cgagcaggtc
     1981 gcgcgggcca acaccgccat cttcaccacg gcgcagaaca accccgaatg cgccgggatg
     2041 ggcaccaccc tggtggtgtg cctcttctac gacgacttcc tgtgcgtggc ccacatcggc
     2101 gactcgcggc tctaccggct tcgtggcgag gtgatggagc aggtgacccg cgatcattcg
     2161 cttctgcagg aacaactcga ctccgggctc atcacgccgg aggaggccaa gctgtcgcag
     2221 aacaagaacc tcgtcaccaa ggcgctgggc atcgacccgg cggtcgagcc cgagctgcac
     2281 gtttacgaga ccgagcgcgg ggacatgtac ctcctgtgct cggacgggtt gaacgacatg
     2341 gtcgaggacg aggaaatccg gctcaccctc atcgcactcc ggtccaaccc gaacctgacg
     2401 gtccagcagc tcgtccaggc ggccaacgac aacggcggcc gcgacaacgt ctcggcgatg
     2461 ctcgtcctcg tcgccgagcc cttcgccgtg gcgcgcggct ggctcgcgcg attcaaggca
     2521 ctattcaggt aattcgcaag gaa
//
LOCUS       DASUEV010000192         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_383006, whole genome shotgun sequence.
ACCESSION   DASUEV010000192 DASUEV010000000
VERSION     DASUEV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383006"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /gene="ctaD"
                     /locus_tag="VFV55_06155"
     CDS             <1..1522
                     /gene="ctaD"
                     /locus_tag="VFV55_06155"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX4943917.1"
                     /translation="RWMYTTNHKDIGTMYLWFAFTMFLLGGTMALLIRAELFQPGLQF
                     VNPEFFNSLTTVHGLVMVFGAIMPAFVGFANWLIPMQVGAADMAFPRMNNWSFWLLIP
                     AALLLISSFFVPGGAAGSGWTLYPPLIIQQGMAIDMTILAVHILGASSIMGSINIITT
                     ILNMRAPGMTLMKMPMFVWTWLITAYLLIAVMPVLAGSITMLLTDRHFGTSFFNAAGG
                     GDPTMFLHIFWFFGHPEVYIMILPAFGIVSHIIPAFARKPLFGYASMVYATSAIAILS
                     FIVWGHHMFTVGMPAAGNLFFMYATILIAVPTGVKIFNWLSTMWRGSMTFEAPMLFAI
                     GFIFLFTIGGFSGLVLAIAPVDITLHNTYYVVAHFHYVLVSGALFAIFAGIYFWLPKW
                     TGHMYNEGLAKAHFWISMIGFNIAFFPQHFLGLAGMPRRIPDYALQFAEWNAISTVGA
                     FIFGFSQLLFLYIVVSTIRSGARAAERPWEGADSLEWTHLPTPVPYHTFETAPVVK"
     gene            1519..1647
                     /locus_tag="VFV55_06160"
     CDS             1519..1647
                     /locus_tag="VFV55_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943918.1"
                     /translation="MMQGAPQDTGRGRRGALVSGMVLAAMAVGIYLVVLLKFFAQG"
     gene            1644..2180
                     /locus_tag="VFV55_06165"
     CDS             1644..2180
                     /locus_tag="VFV55_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX4943919.1"
                     /translation="MSTLPADLELRNRQMMRKLLIVVVGMLGFCAALVPMYRQICEAI
                     GITQQRSVSASTNTQVDMSRQVVVEMIASSAGLAWSFEAIDRSVKVHPGELVTVRYRV
                     VNTLNRPVTANAVMNTAPARANRYIEKQACFCFTEQTLAPGESRDMPVVFRVSADAPR
                     DMQTISLSYTFFETRART"
     gene            2177..>2319
                     /locus_tag="VFV55_06170"
     CDS             2177..>2319
                     /locus_tag="VFV55_06170"
                     /inference="COORDINATES: protein motif:HMM:NF022622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEX4943920.1"
                     /translation="MKNAFRAVFWGMLGIRKRAEYEADTQRLKPQHVVAAGLVAAIVF
                     VLLL"
BASE COUNT          340 a          801 c          724 g          454 t
ORIGIN      
        1 ccgctggatg tacacgacca accacaagga catcggcacg atgtacctgt ggttcgcgtt
       61 caccatgttc cttctgggcg gcacgatggc gctgctcatc cgcgccgagc tcttccagcc
      121 cggcctgcag ttcgtgaatc ccgagttctt caactcgctc accacggtgc acggcctggt
      181 gatggtgttc ggcgcgatca tgccggcctt cgtgggcttc gcgaactggc tgatcccgat
      241 gcaggtgggc gccgccgaca tggcgttccc gcgcatgaac aactggagct tctggctgct
      301 gatcccggcc gcgctcctgc tgatctcgtc gttcttcgtg ccgggcggcg cggccggctc
      361 cggctggacg ctctacccgc cgctcatcat ccagcagggc atggcgatcg acatgacgat
      421 cctggcggtg cacatcctcg gcgcctcctc gatcatgggc tcgatcaaca tcatcaccac
      481 gatcctgaac atgcgcgccc cgggcatgac gctcatgaag atgccgatgt tcgtgtggac
      541 gtggctcatc accgcctacc tgctgatcgc cgtgatgccg gtgctcgcgg gctcgatcac
      601 gatgctgctc accgaccgcc acttcggcac cagcttcttc aacgcggccg gcggcggcga
      661 cccgaccatg ttcctgcaca tcttctggtt cttcgggcac ccggaggtgt acatcatgat
      721 cctgccggcc ttcggcatcg tctcgcacat catcccggcg ttcgccagga agcccctctt
      781 cgggtatgcc tcgatggtgt acgccacctc ggcgatcgcg atcctgtcgt tcatcgtgtg
      841 gggccaccac atgttcacgg tgggcatgcc ggcggcgggc aacctcttct tcatgtacgc
      901 gacgatcctc atcgcggtgc ccaccggcgt gaagatcttc aactggctgt cgacgatgtg
      961 gcgcggctcg atgaccttcg aggcgccgat gctcttcgcc atcgggttca tcttcctctt
     1021 caccatcggc ggcttctcgg gcctggtgct cgccatcgcg ccggtggaca tcacgctgca
     1081 caacacctac tacgtcgtgg cgcacttcca ctacgtgctg gtgtcgggcg cgctcttcgc
     1141 catcttcgcg ggcatctact tctggctgcc gaagtggacc ggccacatgt acaacgaggg
     1201 cctcgccaag gcgcatttct ggatctcgat gatcggcttc aacatcgcct tcttcccgca
     1261 gcacttcctg gggctggcgg gcatgccgcg ccgcatcccg gattacgcgc tgcagttcgc
     1321 cgagtggaac gccatctcga cggtgggcgc gttcatcttc ggcttctcgc agctcctgtt
     1381 cctgtacatc gtggtctcta ccatccgcag cggcgccagg gcggcggaga ggccctggga
     1441 gggcgccgat tcgctcgagt ggacgcacct gccgacgccg gtgccgtacc acacgttcga
     1501 gaccgcgccg gtggtgaagt gatgcagggc gcgccgcagg acacgggacg cggccgcagg
     1561 ggcgcgctcg tctcgggcat ggtgctcgcc gcgatggcgg tgggcatcta cctcgtcgtg
     1621 ctgctcaagt tcttcgccca gggatgagca cgctgcccgc ggatctcgag ctgcgcaacc
     1681 gccagatgat gcgcaagctg ctcatcgtcg tggtgggcat gctcggtttc tgcgcggcgc
     1741 tggtgccgat gtaccgccag atctgcgagg cgatcggcat cacgcagcag cgctccgtgt
     1801 cggcctcgac caacacgcag gtggacatga gccgccaggt ggtggtcgag atgatcgcct
     1861 ccagcgcggg tctcgcctgg agcttcgagg ccatcgaccg ctcggtgaag gtgcatccgg
     1921 gcgagctcgt caccgtgcgc taccgcgtcg tcaacacgct caaccgcccg gtgaccgcca
     1981 acgccgtcat gaacacggcg cccgcgcgcg ccaaccgcta catcgagaag caggcctgct
     2041 tctgcttcac cgagcagacc ctcgcgccgg gcgagtcgcg cgacatgccg gtggtgttcc
     2101 gcgtgagcgc ggacgcgccg cgcgacatgc agaccatctc gctgtcgtac acgttcttcg
     2161 agaccagggc gcggacgtga agaatgcctt ccgcgccgtc ttctggggca tgctgggcat
     2221 ccgcaagcgc gccgagtacg aggcggacac gcagcgcctc aagccccagc atgtcgtcgc
     2281 cgcggggctg gtggccgcca tcgtcttcgt gctgctgct
//
LOCUS       DASUEV010000193         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_980303, whole genome shotgun sequence.
ACCESSION   DASUEV010000193 DASUEV010000000
VERSION     DASUEV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980303"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFV55_06175"
     CDS             <1..945
                     /locus_tag="VFV55_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX4943921.1"
                     /translation="LDLASRIAARLGAAAPEHTVHALDRVDLAVGAGEVVGLVGESGC
                     GKSTLGRIVARILEPTEGELRWKDRPYSDYDASASRSERLAVQMVFQDPFASLNPRMR
                     IVDVIGEAPVVHGLVARGDRDAYVAEIMERVGLDASLARRFPHQFSGGQRSRIGIARA
                     LAVKPRFLVCDEAVAALDVSIQAQVLNLFIRLREEFGLTYLFISHDLGVVRHLSDRVV
                     IMYLGRIVESAPADEVFSRANHPYTQALLAGIPRLEATKRAYIPIKGEIPSPLDPPRG
                     CHFHPRCLHAFARCQAEAPRLREIAPGHLSACHLNDTP"
     gene            942..1991
                     /locus_tag="VFV55_06180"
     CDS             942..1991
                     /locus_tag="VFV55_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX4943922.1"
                     /translation="MSPHHAYLRTDCAGARAPVVLDSPHSGTVYPDDFRHAVPMSALR
                     KAEDAFVDELYACGPQHGATLIAACFPRSYIDPNRSLLDVDTSLLDAPWPGPAAVTRK
                     TELGIGLIWRVLDTGEPIYARKLSVDEVRRRITEFHQPYQKAVKDALDATFEHFGSVW
                     HVNCHSMPAVSSMISEEGPGRPRADFVLGDRDGTTCDPGFTAFVAGLLSDMGYDVKVN
                     DPYKGVELVRAFSDPAAGRHSLQVEVNRRLYMDERTGEKTPGFARLARDIERMVKSIC
                     GYAAERGSHRCDGHDDHDHAHGHCDHDHHHGHDHGHHHDHEHDHSHDHDHVHGHHADP
                     AHDHGSDHLPRKVGN"
     gene            1997..2950
                     /locus_tag="VFV55_06185"
     CDS             1997..2950
                     /locus_tag="VFV55_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX4943923.1"
                     /translation="MTRIAIELAAPVIDSHRRSSTGTDFVHTFESGRPGPHAMVNAIT
                     HGNEICGALAVDRLLRSGLRPVRGTLTLSFANIEAFSRFDPQRPYATRFIDEDFNRVW
                     NAQTLDGPRDSIELRRARELRPFVEAADFLLDIHSMLEPGPPVMICGPLDKGIRFAFD
                     VAIPKDVVSDEGHANGTRMRDFGGFGDPASPKNALLVECGQHWERAAEHVAWQTTWRF
                     LRHVDVVDPEIAVREIAREAPAPQRLVRVTEAVIARTADFRFAREFSGMEVVGQAGDV
                     IAYDGEVTVRAPYDNCVLVMPVPNNVKTGLTAVRLGRIIQR"
     gene            complement(2957..4240)
                     /locus_tag="VFV55_06190"
     CDS             complement(2957..4240)
                     /locus_tag="VFV55_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4943924.1"
                     /translation="MPANQILVVDDEVGIRELLFEILRDEGYGVRLAENAQTARNLRK
                     EMRPDLVLLDIWMPDTDGITLLKEWAGSGLLTMPVVMMSGHGTIDSAVEATRIGAFDF
                     LEKPISLPKLLATVGKALAGGRVLPRAGLAVPQLGKARIMQELRQRLDQVSRLRTPVL
                     LVGEPGCGFEIAARHLHLPNTPWVAPEDNEWLVSNPFGPLNEARDGTLFIPELGVLGK
                     SEQKGLAQLVAKLEKFNVRLVCAASGPVLRMTEDGSFDAALYHQLAGLTIRVPSIDEH
                     TEDVPDLATSLLTSLVDSNEVPFRVLTVGAQNALRNLDWHGNLPVLQNAVKTLALTAL
                     GPEIGGDDVARVAREFSLLPRDQPPTEVGVSLDMPLRDAREQFERQYFLHHIRREDGN
                     MSRVADRVGLERTHLYRKLKQLGIRPSSRGEEPPA"
     gene            complement(4255..>4494)
                     /locus_tag="VFV55_06195"
     CDS             complement(4255..>4494)
                     /locus_tag="VFV55_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4943925.1"
                     /translation="SSLGSDGITLSVRDNGSGIAEAVFGRIFEPYVTTKSKGTGLGLA
                     IVKKIVEEHRGHIMVENVKPHGAHVSIVLPLREAA"
BASE COUNT          708 a         1597 c         1465 g          724 t
ORIGIN      
        1 ctcgatctcg cctcgcgcat cgccgcccgg ctcggcgccg ccgctcccga gcacacggtc
       61 catgccctcg accgcgtcga cctcgccgtg ggtgccggcg aggtggtcgg cctcgtgggc
      121 gaatccggct gcggaaaatc cacgctcggc cgcatcgtgg cccgcatcct cgagcccacc
      181 gagggcgaat tgcgctggaa ggaccgcccc tattccgatt acgacgcttc ggcttcgcgc
      241 agcgaacgcc tggcggtcca gatggtcttc caggacccgt tcgcgtcgct caacccgcgc
      301 atgaggatcg tcgacgtgat cggcgaggcg ccggtcgtcc acggcctcgt cgcgcgcggg
      361 gatcgcgacg cctacgtggc cgagatcatg gagcgcgtcg ggctggacgc gagcctcgcc
      421 cggcgtttcc cgcaccagtt ctccgggggc cagcgtagcc ggatcggcat cgcgcgcgcg
      481 ctcgccgtca agccgcgctt cctcgtgtgc gacgaggcgg ttgcggccct cgacgtttcc
      541 atccaggcgc aggtgctgaa cctcttcatc cggctgcgcg aggagttcgg gctcacctac
      601 ctcttcatca gccacgacct cggggtcgtg cgccacctct ccgaccgcgt cgtgatcatg
      661 tatctcgggc ggatcgtgga atcggcgcct gccgacgagg tcttctcacg cgccaaccac
      721 ccgtacacgc aggccctgct cgccgggatt ccacggctcg aggcgacaaa gcgggcctac
      781 attccgatca agggcgagat tccctcgccg ctggaccccc cccgcggatg ccacttccat
      841 ccgcgctgcc tgcatgcgtt cgcccgttgc caggccgaag cgccacggct gcgcgagatc
      901 gctcccggac atctttccgc ctgccacctg aacgacaccc catgagccca caccacgcct
      961 accttcgcac cgactgcgcc ggcgcacgcg ccccggttgt cctcgactcg ccccacagcg
     1021 gcaccgtcta tccggacgac ttccggcacg cggtgccgat gtccgccctg cgcaaggccg
     1081 aggatgcctt cgtcgacgag ctctacgcct gcggcccgca gcatggcgcg acgctgatcg
     1141 ccgcgtgctt cccgcgctcc tacatcgacc ccaaccggtc cctgctggac gtcgacacct
     1201 cgctcctcga cgcgccgtgg cccgggccgg cggcggtcac ccgcaagacc gagctgggca
     1261 tcgggctcat ctggcgcgtg ctcgataccg gcgagccgat ctacgcgagg aagctctccg
     1321 tcgacgaagt gcggcgccgc atcaccgaat tccaccagcc ctaccagaag gccgtgaagg
     1381 acgcgctgga cgcgaccttc gagcacttcg gatccgtgtg gcacgtgaac tgccattcga
     1441 tgcccgcggt gagcagcatg atctcggaag agggtccggg caggccccgc gcggacttcg
     1501 tcctgggcga ccgcgacggc acgacctgcg atcccggctt caccgcattc gtcgcgggcc
     1561 tgctttccga catgggatac gacgtgaagg tgaacgaccc ttacaagggc gtggagctgg
     1621 tgcgagcctt ctccgacccc gcggccgggc ggcacagcct gcaggtcgag gtgaatcgca
     1681 ggctctacat ggacgagcgc accggcgaga agacgcccgg cttcgcgcgc ctcgccaggg
     1741 acatcgagcg catggtcaag tcgatctgcg gatacgcggc cgagcgcggc tcgcaccgct
     1801 gcgacggcca cgacgaccac gatcatgccc atgggcactg cgaccacgac catcaccacg
     1861 gccacgacca cggccatcac cacgaccacg aacacgatca cagccacgat cacgatcacg
     1921 ttcacggcca tcacgctgat cctgcccacg accacggaag cgaccacctg ccccgaaagg
     1981 tcgggaactg accgccatga cccgcatcgc catcgagctc gccgcacccg tcatcgattc
     2041 ccatcgccgg tcctcgaccg gcaccgactt cgtccacacg ttcgagagcg gccggccggg
     2101 tccgcatgcg atggtgaatg ccatcacgca cggaaacgag atctgcggcg cgctcgccgt
     2161 ggaccgactg ctgcgctccg gtcttcgccc ggtccgggga acgctcacgc tctcgttcgc
     2221 caacatcgag gcgttctcgc ggttcgaccc gcagcgtcct tacgcgacgc gcttcatcga
     2281 cgaggacttc aatcgcgtct ggaacgcgca gacgctggac ggcccgcgcg acagcatcga
     2341 gcttcgtcgc gcgcgcgaat tgcgcccttt cgtcgaggct gcggactttc ttctcgacat
     2401 ccactcgatg ctcgagcccg gtcccccggt gatgatctgc gggccgctcg acaagggcat
     2461 ccgcttcgcc ttcgacgtcg ccatcccgaa ggacgtcgtg agcgacgagg gccatgccaa
     2521 cggaacgcgc atgcgcgact tcggcgggtt cggcgatccg gccagcccga agaatgcgct
     2581 gctggtcgag tgcgggcagc actgggagcg cgccgccgag cacgtggcgt ggcaaacgac
     2641 atggcgattc ctgcgccacg tcgacgtcgt cgatccggaa atcgccgtgc gcgagatcgc
     2701 cagggaggcg ccggcgccgc aacgactggt gcgcgtcacc gaggcagtca tcgcgcggac
     2761 ggcggatttc cgcttcgccc gcgagttttc gggcatggaa gtcgtggggc aggcgggaga
     2821 tgtcatcgcc tatgacggcg aggtgacggt gcgcgccccc tacgacaact gcgtgctggt
     2881 gatgcctgtg ccgaacaacg tgaagaccgg gctcaccgcg gtgcggctgg gtcgcatcat
     2941 ccagcggtag ggcgcctcag gcggggggtt cttcgccccg ggaggagggg cggatgccca
     3001 gctgcttcag cttgcggtag aggtgcgtgc gctcgagccc gacgcgatcg gccacgcgcg
     3061 acatgttgcc gtcctcgcgc cggatatggt gcaggaaata ctgccgctcg aactgctcgc
     3121 gcgcatccct gagcggcatg tcgagcgaca cgccgacctc cgtgggaggc tggtcgcgcg
     3181 gcagcaggga gaattccctc gccacgcggg ccacgtcgtc cccgccgatc tcgggaccga
     3241 gcgccgtcag ggcgagcgtc ttcaccgcgt tctggagcac cggaaggttg ccgtgccagt
     3301 cgagattgcg cagcgcgttc tgcgcgccca ccgtcagcac ccggaaggga acctcgttgg
     3361 agtccaccag ggaagtcagg aggctcgtcg caaggtcagg aacgtcctcc gtgtgctcgt
     3421 cgatcgacgg gacgcggatc gtgagccccg cgagctggtg atagagggcc gcgtcgaagc
     3481 tcccgtcctc ggtcatgcgc aggacggggc ccgacgccgc gcagaccagt cgcacgttga
     3541 atttctcgag cttcgcgacc agctgcgcca ggcccttctg ctcggacttg ccgagcacgc
     3601 cgagttccgg gatgaacagc gttccgtccc ttgcctcgtt cagcgggccg aaggggttgg
     3661 agaccagcca ctcgttgtcc tccggcgcga cccagggcgt attgggaagg tgcaggtggc
     3721 gtgcggcgat ctcgaagccg catcctggtt cgcccaccag cagcaccggc gtgcgcagcc
     3781 ggctcacctg gtcgagccgc tggcggagtt cctgcatgat gcgcgccttc ccgagttgcg
     3841 gaacggcgag gccggcgcgc ggcagcacgc ggccccccgc cagggccttg ccgaccgtcg
     3901 ccagcagctt gggcaggctg atgggctttt caaggaaatc gaacgcgccg atccgcgtgg
     3961 cctcgaccgc cgaatcgatc gttccatgcc ccgacatcat caccaccggc atggtgagga
     4021 ggccggatcc cgcccattcc ttgagcaggg tgatgccatc ggtgtccggc atccagatgt
     4081 cgagcagcac gaggtcgggg cgcatctcct tgcgcaggtt gcgcgcggtc tgggcattct
     4141 ccgccaggcg cactccgtag ccttcgtcgc gcaggatctc gaacaggagt tcgcggatgc
     4201 cgacttcgtc atccacgacc aggatctggt tggccggcat gaagggggcg gcccctaggc
     4261 cgcttcgcgc aggggcagga cgatgctcac gtgtgcgccg tggggcttga cgttctcgac
     4321 catgatgtgg ccgcggtgct cctcgacgat cttcttcacg atcgcgaggc ccagtccggt
     4381 gcccttggac ttggtggtga cgtagggttc gaaaatgcgg ccgaagaccg cttccgcgat
     4441 ccccgatccg ttatcgcgga ccgacagcgt tattccgtcg gaaccgagcg agga
//
LOCUS       DASUEV010000194        20866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_864472, whole genome shotgun sequence.
ACCESSION   DASUEV010000194 DASUEV010000000
VERSION     DASUEV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 20866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20866
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864472"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFV55_06200"
     CDS             <1..931
                     /locus_tag="VFV55_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX4943926.1"
                     /translation="RQPASADASFRRYFRVSSEDRTWIAMDAPPEREDCRPFVKVASL
                     MRAAGVHVPEIVAQDLAQGFLLLTDLGTQTYLQAFDGDNDQRLFRDATAALVDWQAAS
                     REGELPPYDEALLRRELSLFPDWYVVRHLGVTLDATAREDLEKVFRRILASNLAEPRV
                     YVHRDFMPRNLMVSEPNPGVLDFQDAVYGPISYDIASLWRDAFASWEEERVLDGTIRY
                     WEAARKRGLPVRADFADFWHDVEWMGLQRHLKVLGIFARIHYRDGKPGYLDDTPRFIG
                     YVRHACARYGELAPLARLLDRLEDAGVRAGCTS"
     gene            952..1641
                     /locus_tag="VFV55_06205"
     CDS             952..1641
                     /locus_tag="VFV55_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX4943927.1"
                     /translation="MIPPALILAAGRGERMRPLTDTVPKPLLVAGGRPLIAWQLQRLA
                     EAGIRRIVVNHSHLGERLEAAVGDGSRWGVRIAWSREGTALETAGGIVKALDLLGTDP
                     FVVVSGDIYTEFDYSRLAEVAGAIAKDPARRAAHFVLVDNPPFHPRGDMGLAGTRVCR
                     EGPLLTYANIAVFHPSLFAGIAPGTHLRLFPWAYQFVDEGRVTGERFAGTWENLGTPA
                     QLAELDRRLAP"
     gene            1638..2273
                     /locus_tag="VFV55_06210"
     CDS             1638..2273
                     /locus_tag="VFV55_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX4943928.1"
                     /translation="MTFYNPRHFRVDDREALERFIAAHAFGALVSAGRAGLSVSHLPF
                     LPERGASGQLRLLGHMARANGHWQELESAGEVLAIFEGPHAYVSPGWYADHPSVPTWN
                     YVVVHGRGTARLLPPAELPPLLDRLSRAYEDGRPAPWRMNALPPDYTPRLLEAIVGFE
                     IRVERLEGKFKLSQNRRGEDVARVAAALEAEGQGQLAAFMRAQAAPPLESR"
     gene            2322..3638
                     /locus_tag="VFV55_06215"
     CDS             2322..3638
                     /locus_tag="VFV55_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX4943929.1"
                     /translation="MKLENPHHTKSFRQRRAGLARTLREGVVILPTAPERTRNADSHY
                     DYRWDSGFYYLTGFREPEAVMVIVLGRKPREILFCREKNLEREIWDGFRYGPVLAKQA
                     FGFDAAHPVADLDTMIPELIADRSVIHTPVGADPAWDARVTGWLNAVRAKVRTGVSAP
                     AEIRDVRASVNDMRLVKDAHEIAIMRRAAEISGVAHVRAMHAAAPGRREYEIEAELLH
                     EFCRNGARSVAYGSIVAAGANACVLHYRENSAELRKGDLLLIDAGCELDSYAADITRT
                     FPVDGKFTAAQKDVYELVLAAQEAAIKAVKPGAGFIDYHDAATRVLVRGFIDMKLCRG
                     SVDKVLADESYKQFYMHRTGHWLGLDVHDAGDYMRKGKWRKLKPGMVLTVEPGCYIRP
                     ADNVPKHFWNIGVRIEDDVLVTAKGREVLTAGCPKRVTDVEAAVRG"
     gene            3656..4801
                     /locus_tag="VFV55_06220"
     CDS             3656..4801
                     /locus_tag="VFV55_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX4943930.1"
                     /translation="MDKVDVAIVGAGPVGAALAALLVPGGRSVRVLEARSAPSRDART
                     LALSQASRELLEEAGGWPATGVTPIIDIHVSQKGGPGRALITAAEQGLAALGYTVSYA
                     ALEEALEARLTALGTSVSWGAPCEAIDLDAEHARLRLANGESVVARLLVLADGGANAR
                     RIPGLAYSEKDYGQVAITGPVHTDKPHRGRAYERFTPQGPVALLPVAERYALVWTATP
                     DEAARILALDDAAFLAQLQAHFGDRAGRFVAVGPRASFPLKLRTINSTIALRTVIAGN
                     AAQAMHPIAGQGLNIGLRDAASLASILAADPAGDPGTPAALEAYRRSRGRDAGRGVAF
                     TDFLVGAFMDERRLPTLGRGLGLTVLDLLPPARRWLAGRMIHGAPAP"
     gene            4815..5981
                     /locus_tag="VFV55_06225"
     CDS             4815..5981
                     /locus_tag="VFV55_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX4943931.1"
                     /translation="MSTGASDRGRIVVVGGGAVGLAFAAASGDEVRVLESGPPRAAAD
                     PEQYDIRVYALSEGTRSFLREIDAWRRMPAGRMAAVGRMEIFGDDGTSRLEFTPPAGR
                     ALAWIVEGNRLSRAIEEAARDRGATISTQSRLRSIAARPGGAAVVLEDETVIGADLLV
                     GADGADSWVRGELGLAVQAKRYAETAVVAHFRCERPPAGVARQWFRPDGILAWLPLPG
                     ERMSIVWSARDGLAKELAALDPEAFARRVRDAGGAALGDLALDSAIARFPLRLVRVPR
                     ITVPGAVLIGDAAHGVHPLAGQGVNLGFQDARNLARTLGTRSPLERPGDFALLRRHER
                     ERRAGVDAMQFVTDRLEWLFRIDASVAGLARNAGLRAVDAAPAAKRLLAARAMR"
     gene            6053..6778
                     /locus_tag="VFV55_06230"
     CDS             6053..6778
                     /locus_tag="VFV55_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX4943932.1"
                     /translation="MNFRASLSAAFIALLISFPAAAQDLERIKSDLKKKFPEAQIDST
                     RKSGYGGLIEVIGGGEIFYTDEKTSFLLLGHLVDTKSRENVTEARLRTISAVKFDSLP
                     LESAIKIVRGNGSRRIAMFEDPNCGYCKRFERDLQAVNDITVYVFLYPILSPDSTVKS
                     KAVWCAADRGKAWQDMMLKETVPSGAATCEHPIDKIVAYGREKRISGTPTIFFEDGER
                     VPGAMTIAAFEKRLADAKAAAKP"
     gene            complement(6835..8133)
                     /locus_tag="VFV55_06235"
     CDS             complement(6835..8133)
                     /locus_tag="VFV55_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX4943933.1"
                     /translation="MPLATVFPAPRLRAAASLWLVLLAAACATPPVPVAQKAPEALIC
                     PPVEKPVCPTLPPPAPSAPAAPTPVPEVEYRGRLQPASWMDIPDWGREPLRDSLLAFL
                     RGCGALAKQPAWQSVCAVATALPPDATERDISLFFESQFEPYQVINADESTAGMVTGY
                     YEPLLNGSRTRTSRYRYPIYAVPQDLLVIDLSSVYPDLKHKRLRGRIEGNRVVPYLAR
                     GDIESDPAPLKGLEIAWVDDAVELFFLHIQGSGQVQLENGERLRVGYADQNGHPFRSL
                     GRMLIQRGELPPERASMQGIKDWARRHPGKVQRFMNENPSFVFFRELPGDLPGPIGSL
                     GVPLTAERSIAVDPRVIPLGVPVYLATTWPNSPQPLNRLMVAQDTGGAINGGVRADFF
                     WGFGDAAGSQAGRMRQAARMWVLLPKGYTPPSTVPAGAKT"
     gene            8268..8405
                     /locus_tag="VFV55_06240"
     CDS             8268..8405
                     /locus_tag="VFV55_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943934.1"
                     /translation="MGWVLLEVAAALAIAVGIVWWTMPRKPKAREGAGEPADKDGTPR
                     A"
     gene            complement(8402..8785)
                     /gene="apaG"
                     /locus_tag="VFV55_06245"
     CDS             complement(8402..8785)
                     /gene="apaG"
                     /locus_tag="VFV55_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX4943935.1"
                     /translation="MPESKKYVVAVTAHSTYLPDQSDEEDDRFVFAYTIRITNTGNVT
                     AQVVSRHWIITDADNQVQEVRGMGVVGEQPVLKPGDTFEYSSGSSIPTAVGTMRGSYQ
                     LVAEDGTRFEAPIPEFALSVPRVLH"
     gene            8873..9532
                     /gene="rpe"
                     /locus_tag="VFV55_06250"
     CDS             8873..9532
                     /gene="rpe"
                     /locus_tag="VFV55_06250"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX4943936.1"
                     /translation="MSRIAPSLLSADFARLGEEVREVIAAGADLMHFDVMDNHYVPNL
                     TIGPLVCEAIRPHCSVPIDVHLMVKPVDRIIPDFAKAGASIISFHPEASEHIDRTLSF
                     IRDNGCKAGLVFNPATPLAWLDHVMDKVDLVLIMSVNPGFGGQSFIASALPKIREARR
                     RIEASGRDIWLEVDGGVKADNIAAVAKAGADTFVAGSAIFGERDRAAAIRRMRDALAG
                     P"
     gene            9535..10218
                     /locus_tag="VFV55_06255"
     CDS             9535..10218
                     /locus_tag="VFV55_06255"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF009695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX4943937.1"
                     /translation="MPTGSPGPFRAALFDLDGTLVDTALDLAAAANRMLADLGRPALG
                     EEEIRNYIGKGVVNLVQRCVDATGGGFEDERRHALEVFERHYIAGIADRSRPYPGVVD
                     GLRALERAGIAMGCVTNKAGRFTEPLLELTGLRRFFGVVVSGDTVGRKKPHADPLLYA
                     ADRLGATPGETLMVGDSLNDVQSARAAGCPVVVVPYGYREGLALEDLGADAVVASVEE
                     AARSITMAA"
     gene            10346..11824
                     /gene="trpE"
                     /locus_tag="VFV55_06260"
     CDS             10346..11824
                     /gene="trpE"
                     /locus_tag="VFV55_06260"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX4943938.1"
                     /translation="MTENEFLALARAGYNRIPLTLETLADLDTPLSLYLKLANAPGSY
                     LLESVIGGERFGRYSIIGLAARERIEVANGRARVIRDAAVVEERDTGDPLAFVDEYLK
                     QVSAAPLASGLRFTGGLAGYFSYDTVRYIEPKLAHDSRPDPVGLPDIRLLLSEELAVV
                     DNLAGKVHLIVYADAREPNAYGRARARLELLRGRLRGAASLPDHVPAGDVEPPRSNIG
                     EEPFYAAVARAKRYITEGDVMQVQVSQRLTQRFAGSPLNLYRALRSINPSPYMFYFDF
                     GDSQLVGASPEILVRREGDKVTLRPIAGTRKRGATPEKDLAMERELVSDPKERAEHLM
                     LIDLGRNDIGRIARIGTVRVTEQMAVERYSHVMHMVSNVEGEVDPGLTPMQLLRATFP
                     AGTVAGAPKVRAMEIIDELEPERRGVYAGAVGYLGFNGNLDLAIAIRTGVVKSGELHV
                     QAAAGIVADSIPQQEWLETRNKARALLAAAELMNAGLDAPAA"
     gene            11835..12401
                     /locus_tag="VFV55_06265"
     CDS             11835..12401
                     /locus_tag="VFV55_06265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX4943939.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGEEVRVARNDEITLEEIARLAP
                     ARIVISPGPCTPNEAGVSLPLIARFAGKIPILGVCLGHQAIGQAFGGHVVHAKTLMHG
                     KVSPIHHGGQGVFRGLPSPFNATRYHSLAVERESCPADLEITAWTEDGEIMGVRHRAL
                     AVEGVQFHPESILTEHGHALLRNFLTQE"
     gene            12404..13441
                     /gene="trpD"
                     /locus_tag="VFV55_06270"
     CDS             12404..13441
                     /gene="trpD"
                     /locus_tag="VFV55_06270"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX4943940.1"
                     /translation="MFTAHEAINRLCDKREIFYDEMVDLMRQVMEGKVTPVQLAAILM
                     GLHVKTESVSEIAAAAAVMREFSTKVDASGLDHLVDTCGTGGDKCHTFNISTTAAFVA
                     AAAGARVAKHGGRAVSSKSGSADVLEGLGVNLALTPEQEALCIREVGVGFMFAPSHHP
                     AMKHAAPVRKELGMRTILNILGPLTNPAGAPNQVMGVFHTDLVGIQARVLKMLGSRHV
                     LTFHGQDGLDEITITGPTNVAELKHDFITEYVVEPRQFGLDNAPIEAIQVAGPEESRA
                     RVLAVLAGEAGPSRDIVLLNAAAALYVSGVAASLWDGVALAREAIASGGAKAKLDHLV
                     AFTQTFAASGS"
     gene            13438..14232
                     /gene="trpC"
                     /locus_tag="VFV55_06275"
     CDS             13438..14232
                     /gene="trpC"
                     /locus_tag="VFV55_06275"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX4943941.1"
                     /translation="MSDILQKILATKRREVEAARIAVPLPELERRARAAQSPRGFTAA
                     LQARIAAGKPAVIAEIKRASPSRGLIRADFDPARIAISYAANGAACLSVLTDREYFGG
                     SREDLEAARAACALPVIRKDFIIDAYQVIESRAWGADCILLIVDAVSDSSLDQLEALA
                     ISLGMDVLVESHDAGQLERAIRLRTPLLGVNNRDLRNFEIRLETSLELANRIPAGKIL
                     VAESGIASPADVERLRNGKVSAYLVGSAFMAAADPGAELSRLFSGL"
     gene            14438..15475
                     /locus_tag="VFV55_06280"
     CDS             14438..15475
                     /locus_tag="VFV55_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX4943942.1"
                     /translation="MNKKLMALAVAGACVAPTAMAQTANPVTLYGRVYVNIQSVEAKG
                     GPAASNASNRMQVSDDSSLLGVRGTEDIGGGNKVFFQLETAFKPDSNAAVSNTFAQRN
                     SAVGLQGGWGSLLAGRWDSPYKVLTYGADIFGDLNLGGITAALHDRGNFDVRQQNVIQ
                     YWTPNFAGFTVKLAGTANEGKTASLNPRQFAGNLVWSRGPIALSYAYEEHKDVSATIR
                     KETGNEGVVEFKFGAFKVSGIYEEIKKDGTTKQKNYLASLLWTAGKHQVGYQFSNSKD
                     GGANGAETQPDCDVNAVGYFYNWSKRSQFVATYVKVDNNAASVCKFGSNDVGGAGADR
                     EGFGVGIKHVF"
     gene            15562..16185
                     /gene="coq7"
                     /locus_tag="VFV55_06285"
     CDS             15562..16185
                     /gene="coq7"
                     /locus_tag="VFV55_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX4943943.1"
                     /translation="MTALDRVLIVADNALRTLTGSVTPSRAAPGEKDANPALPEERRD
                     VAGLMRVDHAGEVCAQALYAGQSLVARDGRVRRALERAGAEERDHLAWCAQRLRELDS
                     RPSVLSPIWYAGSFALGIVSGAAGDRWSMAFLVETERQVEKHIDGHIERLPPGDARSR
                     AILEQMREDEIGHANTGRSLGAGELPLPVRSAMRLVAKLMTVTAYRV"
     gene            complement(16205..16621)
                     /locus_tag="VFV55_06290"
     CDS             complement(16205..16621)
                     /locus_tag="VFV55_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4943944.1"
                     /translation="MKARVKLVEGMTFVAESGSGHSVVMDAAPDVGGRDLGARPMEMV
                     LMGAGGCSAIDVVHILGKARQDLVDCVVELEADRAAEDPKVFTRIHLHYVVTGRGLNP
                     AQVERAIKLSKDKYCSATIMLARTAEITSDYEIREA"
     gene            complement(16618..17676)
                     /locus_tag="VFV55_06295"
     CDS             complement(16618..17676)
                     /locus_tag="VFV55_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX4943945.1"
                     /translation="MDSSRFEQYARVAGIALLVAGCFLVIRPFLAALFFAAVMCLSTW
                     PAFRWIKERVGGRSTLAALLFALGMLLAIALPVALAAYSLITHSADAIDLIRGFLDRG
                     PIELPGWLTRIPFVGGWIDDYWKVLVGSREEMVALGKRLAEPAKNVLFAAGASAGEGV
                     LQILLAIFVAFFFYRDGEVVARMIRTGMARLAGGERGASVLATAQNAIKGVVYGLIGT
                     ALAQAAVSLLGFLVAGVPAAMVLAALTFVLSLVPMGPVIIWGGAAAWLYFQGETGWAV
                     FMVIYGTLVISSVDNFVKPILMSRAGGLSMLLVVLGVFGGAVAFGFIGIFVGPALLAV
                     AWSLVNAWLEAHLLREAV"
     gene            17880..18308
                     /gene="rplM"
                     /locus_tag="VFV55_06300"
     CDS             17880..18308
                     /gene="rplM"
                     /locus_tag="VFV55_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX4943946.1"
                     /translation="MKTFSAKPREVRRDWYVVDATDKVLGRLAVEVSTRLRGKHKPEF
                     TPHVDTGDYIVVVNVEKLRVTGAKSSDKMYYRHSTYPGGIYETTFNKLQARHPDRVLK
                     LAVKGMLPKGRLGYAMIKKLKIYAGPAHPHTSQQPKALEI"
     gene            18321..18713
                     /gene="rpsI"
                     /locus_tag="VFV55_06305"
     CDS             18321..18713
                     /gene="rpsI"
                     /locus_tag="VFV55_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX4943947.1"
                     /translation="MIGKYNYGTGRRKNAVARVFIKSGQGKIVVNEKPLDEYFSRETG
                     RMIVRQPLELTNHAQTFDIKVSVFGGGESGQAGAVRHGITRALIEYDATLKPALSKAG
                     LVTRDAREVERKKVGFHKARRRKQYSKR"
     gene            18830..19858
                     /gene="argC"
                     /locus_tag="VFV55_06310"
     CDS             18830..19858
                     /gene="argC"
                     /locus_tag="VFV55_06310"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX4943948.1"
                     /translation="MIRIGVVGGTGYTGVELLRLLATHPDVEIVAITSRGEAGTPVAR
                     IFPSLRKHVDIAFSDPAATDLTACDCVFFATPNGVAMGQVRPLLDAGVKVIDLSADFR
                     LKDIATWERWYRMKHAAPDLVSEAAYGLPEINRDKIRGARIVANPGCYATAVQLALLP
                     LVETDFVDRAHFIADAKSGVSGAGRKAEVDLLFSEASDNFKAYAVGGHRHHPEIVQSL
                     RQAGGNEVGLAFVPHLVPMIRGIFATVYARVTGEADFQQLFERRYADEAFVDVLPPGT
                     QPETRWARGSNVCRIAVHRPMDGDTLVVLAVEDNLVKGAAGQAVQNMNIMFGLPEGRG
                     LEQVALLP"
     gene            19855..20601
                     /locus_tag="VFV55_06315"
     CDS             19855..20601
                     /locus_tag="VFV55_06315"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEX4943949.1"
                     /translation="MRSLTRRLRSNFGIRSSRMTIRSHLAWYWRWLLNVLMMAAVAGI
                     VWWLVENSYRITGFNREETKQQIAAFGEENARLKRELEAARVVVAERDRQSAIDKAAQ
                     MELARNVAQLQDENATLKEDLGFLRNIMSSGTTPEGLGIANLKVEQDGQPNEYRYRML
                     LTQGGQRKQDFKGKVQVLARVAQGGAESTLTFPEPSAGDRVGDVEFRFYQKLDGRFRI
                     PTGAVLRSCEVRILALPGGQVKLSRSVNVQ"
     gene            20611..>20866
                     /locus_tag="VFV55_06320"
     CDS             20611..>20866
                     /locus_tag="VFV55_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX4943950.1"
                     /translation="MFGKGNKPSPIDSLIGAGTVIEGNVTFVGGLRIDGQVKGSVKAT
                     GSKPGTLVLSELAKVEGEIDVAHVVVNGTVAGPVRATEYVE"
BASE COUNT         3520 a         6977 c         7090 g         3279 t
ORIGIN      
        1 gcgccagccg gcgagcgccg acgcgagctt ccgccgttat ttccgcgtct cctccgagga
       61 ccggacctgg atcgcgatgg acgcgccacc cgaacgcgag gattgccggc cgttcgtgaa
      121 ggtcgcatcc ctcatgcggg ctgccggcgt gcacgtgccg gagatcgtcg cgcaggacct
      181 cgcgcagggc ttcctcctgc tcaccgatct cggcacgcag acctacctgc aggccttcga
      241 cggggataac gaccagcgcc tcttccgcga cgccaccgcg gctctcgtcg actggcaggc
      301 cgccagccgg gaaggggaac tgccgcctta cgacgaggcg ctccttcgtc gcgagctttc
      361 gctcttcccg gactggtacg tcgtgcgcca cctcggggtc acgctcgacg ccaccgcgcg
      421 cgaggacctg gagaaagtct tccggcgcat cctcgcctcg aatctcgccg agccccgcgt
      481 ctacgtgcac cgcgacttca tgccgcgaaa cctcatggtg tcggagccca accccggcgt
      541 gctcgacttc caggacgccg tatacggccc gatcagctac gacatcgcct ccctctggcg
      601 cgacgccttc gcgagctggg aggaggagcg cgtcctcgac gggaccatcc gctactggga
      661 ggcggcgcga aagcgcggcc tgccggtgcg cgccgacttc gccgacttct ggcacgacgt
      721 ggaatggatg gggctgcagc ggcacctgaa ggtgctgggc atcttcgcgc gcatccacta
      781 ccgcgatggc aagcccggct atctcgacga cacgccgcgc ttcatcggtt acgtgcgcca
      841 cgcctgcgcg cgctacggcg agctagcgcc gctggcgcgg cttctcgacc gcctcgagga
      901 tgcgggcgtg cgcgccggtt gcacgtcctg atgcgcccgg caggcgatgc gatgattccc
      961 ccggcgctca tcctggcggc cggccgcggc gagcgcatgc gcccgctcac cgacaccgtt
     1021 cccaagccgc tcctcgtcgc cggcggcagg cccctcatcg cctggcagct gcagcgcctg
     1081 gcggaggcgg gcatccgccg gatcgtcgtc aatcactcgc acctgggtga aaggctcgag
     1141 gccgcggtgg gcgacgggtc gcgatggggc gtgcgcatcg cgtggtcccg ggaaggcacg
     1201 gcgctcgaga ccgcgggggg catcgtgaag gccctcgacc tgctcggcac ggatccgttc
     1261 gtggtcgtca gcggcgacat ctataccgaa ttcgattatt cgcgcctcgc ggaggtggcc
     1321 ggtgcgatcg cgaaggaccc ggcgcggcgc gccgcgcatt tcgtgctggt cgacaatccg
     1381 ccgttccatc cgcgcggcga catgggactt gcgggcactc gcgtctgccg cgaagggccc
     1441 ttgctcacct atgcgaacat cgcggtgttc catccgtcgc tgttcgcggg catcgcgccg
     1501 ggcacgcacc tcaggctctt tccctgggct taccagttcg tcgacgaagg gcgcgtcacc
     1561 ggcgagcgtt tcgccggcac gtgggagaac ctcggcacgc ccgcccagct ggcggaactc
     1621 gacaggaggc ttgcgccatg acgttctaca acccgcgcca tttccgcgtc gacgaccgcg
     1681 aggcgctcga gcgcttcatc gcggcgcacg cgttcggcgc gctcgtttcc gccggccgcg
     1741 cgggactttc cgtgagtcac cttccattcc tgccggagcg cggcgccagc gggcagcttc
     1801 gccttctcgg gcacatggcg cgcgcgaacg ggcattggca ggaactggag tccgcgggcg
     1861 aggtcctggc gatcttcgag ggaccgcatg cgtacgtttc gcccgggtgg tatgccgacc
     1921 accccagcgt gccgacatgg aactatgtgg tggtgcacgg gcgcggcacg gcccgcctgc
     1981 tgccgcccgc ggaactgccg ccgctgctcg atcgcctgtc gcgggcctac gaggacggga
     2041 ggcccgcgcc gtggcggatg aacgccctcc ctcccgacta cacaccgcgg cttctcgagg
     2101 cgatcgtggg gttcgagatc cgcgtggagc ggctcgaggg caagttcaag ctctcgcaga
     2161 accgccgcgg cgaggacgtc gcgcgcgtgg ccgccgcgct cgaggccgaa ggccagggcc
     2221 agctggcggc gttcatgcgc gcccaggcgg ccccgcccct cgaatcccgg tagaatcggc
     2281 ggcctctcgg ccgtctccct cgtgcccggc acccataacc catgaagctc gaaaatcccc
     2341 atcacaccaa gtccttccgc cagcgccgtg cgggcctggc ccggacgttg cgcgaaggcg
     2401 tcgtgatcct gccgaccgcg cccgaacgca cgcgcaatgc cgactcccat tacgactacc
     2461 ggtgggacag cggcttctac tacctcaccg gcttccgtga gcccgaggcc gtcatggtga
     2521 tcgtgctcgg caggaaaccc cgcgaaatcc tcttctgccg cgagaagaat ctcgagcgcg
     2581 agatctggga cgggtttcgc tacggtcccg tgctcgcgaa gcaggcattt ggcttcgacg
     2641 cggcgcaccc ggtcgcggac ctggatacga tgatccccga gctcatcgcc gaccggtcgg
     2701 tgatccacac gccggtgggc gcggaccctg cctgggacgc gcgcgtgacg ggctggctca
     2761 atgccgtgcg tgccaaggtg cgcacgggcg tctcggcccc cgccgagatc cgcgacgtgc
     2821 gcgcgagcgt gaacgacatg cgcctcgtca aggacgcgca cgagatcgcg atcatgcgcc
     2881 gtgccgcgga gatttcgggc gttgcgcacg ttcgcgcgat gcacgcggcc gctcccggca
     2941 ggcgcgaata cgagatcgag gcggagctgc tgcacgagtt ctgccgcaac ggggcccgct
     3001 ccgtggccta cggctccatc gtggccgcgg gcgccaacgc ctgcgtgctg cattaccgcg
     3061 agaactccgc ggagctgcgc aagggcgacc tcctgctcat cgacgccggc tgcgaactcg
     3121 acagctacgc cgccgacatc acgcgcacgt tcccggtgga cgggaagttc accgcggcgc
     3181 agaaggacgt ttacgagctg gtcctggcgg cgcaggaggc ggccatcaag gcggtgaagc
     3241 cgggcgcggg cttcatcgac taccacgacg ccgccacgcg cgtgctggtg cgcggattca
     3301 tcgacatgaa gctgtgcagg ggcagcgtcg acaaggtgct ggccgacgag tcgtacaagc
     3361 agttctacat gcatcgcacc ggccactggc tcgggctcga cgtgcacgac gcgggtgact
     3421 acatgcgcaa gggcaagtgg aggaagctca agcccggcat ggtgctcacc gtggagccgg
     3481 gttgctacat ccgcccggcc gacaacgtgc cgaagcattt ctggaacatc ggcgtgcgca
     3541 tcgaggacga cgtcctcgtc accgcgaagg gccgcgaggt cctgacggcc gggtgcccga
     3601 agcgcgtgac cgacgtcgag gccgcggtcc ggggttgacc cggaaggcgg gcccgatgga
     3661 caaggtcgac gtcgcgatcg tcggggccgg gccggtcggc gccgcgctcg ccgcgctgct
     3721 ggtgccggga gggcgctccg tgcgcgtcct cgaggcgcgc agcgcaccct cccgggatgc
     3781 gcgcaccctt gccctctccc aggcgagccg cgagcttctc gaggaagccg gcggctggcc
     3841 cgcgaccggc gtgacaccca tcatcgacat ccatgtctcg cagaagggcg gcccggggcg
     3901 cgcgctcatc acggcggcgg agcaggggct tgccgctctc gggtacacgg tttcctacgc
     3961 ggcgctcgag gaagcactgg aggcgcgcct cacggccctc ggcacgagcg tgtcgtgggg
     4021 cgcgccctgc gaagccatcg atctggacgc ggagcacgcg aggctgcggc tggcgaacgg
     4081 cgaaagcgtg gtggcgcggc tgctcgtgct cgccgacggc ggcgccaatg cgcggcgcat
     4141 tccggggctc gcctattcgg agaaggacta tgggcaggtg gcgatcaccg ggcccgtgca
     4201 caccgacaag ccgcaccgcg gccgtgccta cgagcgattc acgccgcagg ggccggtggc
     4261 gctgctgccg gtagcagagc gctacgccct cgtatggacc gcgacaccgg acgaagcggc
     4321 gcgcatcctc gcgctggacg atgccgcgtt cctcgcgcag ttgcaggcgc acttcggaga
     4381 ccgcgcggga cgattcgtcg cggtggggcc gcgggcgtcg tttcccctca agctgcggac
     4441 catcaactcc acgatcgccc tgcgcacggt gatcgccggc aacgcggcgc aggcgatgca
     4501 ccccatcgcc ggtcagggac tcaacatcgg cttgcgcgac gccgcctcgc tcgcgtcgat
     4561 tctggcggcc gatcccgccg gcgatcccgg cacccccgcg gcgctcgaag cctatcgccg
     4621 ttcacgcggt cgcgatgccg gacgtggcgt cgcattcacc gatttcctcg tgggcgcctt
     4681 catggacgaa cgccgcctgc cgaccttggg tcgcggcctc gggctcaccg tcctcgatct
     4741 cctgcctcct gcgcggcgat ggctggcggg caggatgatc cacggagcgc ccgcgccgtg
     4801 agccgcggct accgatgagc accggcgcaa gcgaccgcgg ccggatcgtc gtggtcgggg
     4861 gaggggcggt gggcctggcc ttcgcggcgg cttccggaga cgaggttcgc gtgctcgaaa
     4921 gcggcccgcc gcgcgctgcg gcggacccgg agcagtacga catccgcgtc tatgcgctca
     4981 gcgaaggcac gcgctcgttc cttcgcgaga tcgatgcctg gcggcgcatg ccggccggca
     5041 ggatggccgc cgtcggtcgc atggagattt tcggcgacga cggcacgagc cggctcgagt
     5101 tcacgccgcc cgccggccgc gcgcttgcct ggatcgtcga aggcaaccgc ctgtcgcgcg
     5161 cgatcgagga agcagcacgc gatcgcggcg cgacgatttc gacgcaatcc cggctgcgtt
     5221 cgatcgccgc gcgccccggg ggggccgcgg tcgttctcga ggacgaaacg gtgatcggcg
     5281 cggatctcct ggtgggagcg gacggggcgg actcgtgggt gcgcggggaa ctcggtctcg
     5341 ccgtacaggc gaagcgctac gccgagacgg cggtcgtggc gcacttccgc tgcgagcggc
     5401 cgccggcggg cgtggcccgg caatggttcc ggccggacgg catcctcgcc tggctgccgc
     5461 tgcccgggga gcggatgtcg atcgtgtggt cggcgcgcga cggcctggcc aaagagctgg
     5521 ccgcactcga ccccgaagcc ttcgcccgcc gagtgcgcga tgcgggaggc gcggcgctgg
     5581 gcgatctcgc gctcgattcg gccatcgccc ggttcccgct ccggctggtt cgggtgccca
     5641 ggatcacggt gccgggcgcc gtgctcatcg gggatgccgc acacggcgtt catcccctcg
     5701 cgggccaggg cgtgaacctc ggtttccagg acgcgcgcaa ccttgcgcgc accctcggca
     5761 cgcgctcgcc cctcgagcgg cccggcgatt tcgcgctgct gcgacgccac gagcgcgagc
     5821 ggcgggccgg cgtggatgcc atgcagttcg tcaccgatcg cctcgagtgg ctcttccgca
     5881 tcgacgcgtc ggtggccggt ttggcgcgca acgcggggct gagggccgtc gatgcggcgc
     5941 cggcggccaa gcgtctcctg gcggcgcggg caatgcgata atgatccttc cgcaccgggg
     6001 aacttacccg gccggtcacg gcccaacccc cggtatccac agagacccct ccatgaactt
     6061 ccgtgcctcc ctttccgccg ccttcatagc cctcctgatc tcctttcccg ccgcagcgca
     6121 ggatctcgag cggatcaagt ccgacctcaa gaagaagttc ccggaggcgc agatcgactc
     6181 gacgcgcaag agcggctacg gcggcctcat cgaggtgatc ggcggcgggg agatcttcta
     6241 taccgacgag aagacgtcgt tcctgctgct gggccacctc gtcgacacga aatcgcgcga
     6301 gaacgtgacc gaagcgcgcc tgcgcacgat ctcggccgtc aagttcgaca gcctgccgct
     6361 ggaatccgcg atcaagatcg tgcgcggcaa cggctcgcgg cgcatcgcga tgttcgagga
     6421 tcccaactgc ggatactgca agcggttcga gcgcgacctc caggccgtga acgacatcac
     6481 ggtctacgtg ttcctctatc ccatcctgtc gccggattcg acggtgaagt cgaaggccgt
     6541 gtggtgcgcg gcggaccggg gcaaggcgtg gcaggacatg atgctgaagg agacggttcc
     6601 ctccggcgcg gcgacctgcg agcacccgat cgacaagatc gtcgcctacg ggcgggaaaa
     6661 gcgcatcagc ggcacgccga cgatcttctt cgaggatggc gagcgcgtcc ccggcgctat
     6721 gaccatcgcg gctttcgaaa agcgcctcgc cgacgcgaag gccgccgcca agccctgagt
     6781 acggcgggtt cggcgtccgg agcacgtgac gggcaggccc cggtcatccg ggcattacgt
     6841 cttcgcgccc gccgggacgg tcgagggcgg cgtgtagccc ttcggaagca gcacccacat
     6901 ccgcgcggcc tggcgcatcc tgccggcctg gcttccggcc gcgtccccga atccccagaa
     6961 gaagtcggca cggacgccgc cgttgatcgc accgcccgtg tcctgcgcca ccatgaggcg
     7021 gttgagtggc tgcggcgaat tgggccatgt cgtcgcgagg tagaccggca cgcccagcgg
     7081 aatcacgcgc gggtcgacgg cgatcgagcg ctccgcggtg aggggcacgc cgagcgagcc
     7141 gatcggcccc ggaaggtcgc ccggcagctc gcggaagaac acgaagctcg ggttctcgtt
     7201 catgaagcgc tgcaccttcc cgggatggcg gcgggcccag tccttgatcc cctgcatcga
     7261 ggcgcgctcg ggcggcaatt ccccgcgctg gatgagcatc ctcccgagcg aacggaatgg
     7321 gtgcccgttc tggtcggcgt aacccacccg caggcgttcg ccgttttcga gctgcacctg
     7381 ccccgagccc tggatgtgca ggaagaagag ctcgaccgcg tcgtcgaccc aggcgatctc
     7441 gagtcccttg agcggagccg gatcgctttc gatgtcgccg cgcgcgaggt aggggacgac
     7501 gcgattgccc tcgatccggc cgcgaagccg cttgtgcttg aggtccggat agaccgacga
     7561 gaggtcgatc accagcaggt cctgcggcac cgcgtagatc ggatagcggt aacgggacgt
     7621 gcgcgtgcgg ctgccgttca gcaagggctc gtaatagccg gtgaccatgc ccgcagtcga
     7681 ctcgtccgca ttgatcacct ggtacggctc gaactgcgat tcgaagaaca gggagatgtc
     7741 gcgctcggtg gcatccggcg ggagcgcggt ggccacggcg cataccgatt gccaggccgg
     7801 ctgtttcgcc agtgcgccgc agccgcgaag gaaggcgagg agcgaatcgc gcagcggttc
     7861 gcgcccccag tccggaatgt ccatccacga cgcaggctgc agccggcccc ggtattcgac
     7921 ctcgggaacg ggggttggcg cagcgggcgc ggaaggcgcc ggtggtggca atgtcgggca
     7981 aaccggtttc tcgacaggcg ggcagatgag cgcttcgggg gccttttgcg cgaccggaac
     8041 cggcggcgtc gcgcaggcgg cggcaaggag aacgagccag agactggcgg cggcgcgaag
     8101 gcgcggcgcc ggaaaaacgg tagctagcgg catcgcggcg agtatagcgg aaggctcgtg
     8161 tctcaaaggg gtcaggttac tttacggatc gaccccgccg ctggcggggg aatgacagta
     8221 aagtaacctg acccctttca agcaattcaa gactaccgga gccgcgcatg ggctgggttc
     8281 tcctcgaagt ggcagcagcg ctcgcgatcg cagtcggcat cgtgtggtgg acgatgccgc
     8341 gcaaaccgaa ggcgagggag ggtgccggcg agccggcgga caaggacggg acgccgcgag
     8401 cctagtgcag gacgcgcggg acgctgagcg cgaattccgg aatcggtgcc tcgaagcgcg
     8461 tgccgtcctc cgccaccagc tggtagctgc cgcgcatcgt gcccacggcg gtcgggatgg
     8521 aagacccgct ggaatattcg aacgtatcgc cgggcttgag caccggctgc tcgcccacca
     8581 cccccatgcc gcgcacctcc tgcacctggt tgtccgcgtc ggtgatgatc cagtggcggc
     8641 tcacgacctg tgccgtgacg ttcccggtgt tcgtgatgcg gatggtgtag gcgaacacga
     8701 agcggtcgtc ctcctcgtcc gactggtcgg gcaggtaggt cgaatgggcc gtgacggcga
     8761 ccacgtattt tttcgattcc ggcattcgat gatggtagca ggaatcccgg gacccgaatc
     8821 ggcgcggtaa tcgcgtagaa tttcaccgtc caccgccttc cgaaagtccc ccatgtcccg
     8881 catcgcgccc agcctcctgt ccgcggattt cgcgcgtctc ggcgaggagg tgagggaagt
     8941 catcgcggca ggcgccgacc tcatgcactt cgacgtgatg gacaaccatt acgtgcccaa
     9001 cctcaccatc ggcccgctcg tgtgcgaggc catccggccg cactgcagcg tgccgatcga
     9061 cgtgcacctg atggtgaagc ccgtcgaccg catcatcccc gatttcgcca aggcgggcgc
     9121 ctcgatcatc agcttccacc cggaggcgag cgagcacatc gaccgcacgc tctccttcat
     9181 ccgcgacaac gggtgcaagg cagggctcgt gttcaacccc gccacgccgc tcgcgtggct
     9241 cgaccacgtg atggacaagg tggacctcgt cctcatcatg agcgtgaacc ccggattcgg
     9301 cgggcagtcc ttcatcgcct ccgccctgcc caagatccgc gaggcgcgcc ggcgcatcga
     9361 ggcgagcggg cgcgacatct ggctggaagt cgatggcggg gtgaaggcgg acaacatcgc
     9421 tgccgtggcc aaggccggag cggatacctt cgttgccggc tcggcgatct tcggagagcg
     9481 cgaccgcgcg gccgcgatcc ggcgcatgcg cgacgcgctc gccgggccgt gacgatgccc
     9541 acgggctcgc ccggaccatt ccgtgccgcg ctgttcgacc tggatggaac gctggtcgat
     9601 accgctctcg atcttgcggc ggccgccaac cggatgctcg ccgacctcgg acggcccgct
     9661 ctcggcgagg aggagatccg caactacatc ggcaagggcg tggtgaacct cgtgcagcgt
     9721 tgcgtagatg ccaccggcgg cggcttcgaa gacgaacggc gccatgcgct cgaagtgttc
     9781 gagcgccact acatcgccgg aatcgcggat cgctcgcggc cgtatccggg cgtggtcgac
     9841 ggactccgcg cccttgagcg cgcgggaata gcgatggggt gcgtcaccaa caaggcaggg
     9901 cgcttcaccg agcccctgct cgaactcacg gggctgaggc ggttcttcgg ggtggtggtt
     9961 tccggcgata cggtcgggcg caagaagccc catgccgatc cgctgctcta cgcggcggac
    10021 aggctcggcg ccacgcccgg cgagacgctg atggtcggcg attccctgaa cgatgttcaa
    10081 tccgcgcgcg ccgccggttg cccggtggtg gtggttccgt acggctatcg cgagggcctg
    10141 gcgctcgagg acctcggcgc cgacgcggtc gtggcgagcg tggaagaggc cgccagatcg
    10201 attacaatgg ctgcgtagac cacgcctcac ccccgaacct catcatggca ctgcgcggat
    10261 tcctgctcga ctggcgatgg cacggctggc gctgaacgcg tccggcccag acctgccctt
    10321 cgacccgact accgtgtggt gaaccatgac tgagaacgaa ttcctggcgc ttgcccgcgc
    10381 cggctacaac cggatccccc tcaccctcga gacgctcgcc gatctcgata cgccgctatc
    10441 gctgtacctc aagctagcga acgccccggg ctcgtacctt ctcgagtcgg tgatcggggg
    10501 cgagcggttc ggccgctact cgatcatcgg gctggccgcg cgcgagcgaa tcgaggtggc
    10561 gaacgggcgc gcccgcgtca ttcgcgacgc tgccgtggtg gaggaacgcg atacgggcga
    10621 ccccctcgcc ttcgtggacg aatacctgaa gcaagtttcg gcggcaccgc tcgcctcggg
    10681 actgcggttc accggcgggc tggccggcta tttcagctac gacaccgtgc gctacatcga
    10741 gccgaagctc gcgcacgatt cgcgcccgga tcccgtcggc ctgcccgaca tccgcctgct
    10801 cctctccgag gaactcgccg tggtggacaa cctcgcgggc aaggtgcatc tcatcgtcta
    10861 cgccgatgcg cgcgagccga acgcctacgg gcgtgcgcgc gcgaggctcg agctgcttcg
    10921 cggaaggctt cgcggcgcgg cgtcgttgcc ggatcatgtc ccggcggggg atgtcgagcc
    10981 gccgcgctcg aacatcggcg aagagccgtt ctacgccgcg gtcgcgaggg cgaagcgcta
    11041 catcaccgag ggcgacgtga tgcaggtgca ggtctcgcag cgcctgacac agcgcttcgc
    11101 ggggtccccg ttgaacctct accgggcgct gcgctcgatc aacccctcgc cctacatgtt
    11161 ctatttcgac ttcggcgaca gccaactggt cggtgcctcc ccggagatcc tcgtgaggcg
    11221 cgaaggcgac aaggtgacgc tgcggcccat cgcagggacc cgcaagcgcg gcgccacgcc
    11281 cgagaaggac cttgccatgg agagggagct ggtgagcgac cccaaggaaa gggccgagca
    11341 cctcatgctg atcgatctcg gccgcaacga catcggtcgg atcgccagga tcggcaccgt
    11401 gcgggtcacg gagcagatgg ccgtcgagcg ctattcccac gtgatgcaca tggtctcgaa
    11461 cgtcgagggc gaggtggacc cgggcctcac gccgatgcag cttctgcgcg ccacgtttcc
    11521 ggccggcacc gtggccgggg cgccgaaagt gcgcgcgatg gagatcatcg acgaactgga
    11581 gcccgagcgg cgcggcgtct atgcaggcgc ggtgggctac ctcggattca acggcaacct
    11641 cgacctggcg atcgccatcc gcaccggcgt ggtcaagtcg ggcgagctgc acgtgcaggc
    11701 tgccgccggc atcgtggccg attccatccc gcagcaggaa tggctcgaga cgcgcaacaa
    11761 ggcgcgggca ctgctggcgg ccgccgaact gatgaatgcg gggctcgatg cccccgccgc
    11821 atgaggagcc ggccatgctg ctcatgatcg acaactacga ctcgttcacc tacaacctgg
    11881 tccagtacct cggagaactg ggcgaggaag tccgcgtggc gcgcaacgac gagatcacgc
    11941 tggaggagat cgcccgcctc gcgcccgctc gaatcgtgat ttcgcccggg ccctgcacgc
    12001 ccaacgaggc cggcgtttcc ttgccgctca tcgcacggtt cgccgggaag attcccattc
    12061 tcggggtgtg cctggggcac caggccatcg gccaggcctt cggcgggcac gtggtgcacg
    12121 caaagacact catgcacggc aaggtctcgc cgatccatca tggcggccag ggcgtgttcc
    12181 gcgggcttcc ctcgccgttc aacgcgaccc gctaccattc gctcgccgtg gagcgcgaaa
    12241 gctgccccgc ggatctcgag ataacggcct ggaccgagga cggcgagatc atgggcgtgc
    12301 gccatcgcgc gttggcggtg gagggcgtgc agttccatcc ggaatcgatc ctcacggagc
    12361 acggccacgc gctgctccgc aattttctca cgcaggagta gccatgttca ccgcccacga
    12421 agcaatcaac cgcctctgcg acaagcgcga gatcttctac gacgagatgg tcgacctcat
    12481 gcgccaggtg atggaaggca aggtcacccc ggtgcagctc gcggccatcc tcatggggct
    12541 gcacgtgaag acggaatcgg tgtccgaaat cgccgctgcg gccgcggtga tgcgcgagtt
    12601 ctccacgaag gtggacgcct ccgggctcga ccacctcgtc gacacctgcg gaacgggtgg
    12661 cgacaagtgc catacgttca acatttcgac cacggcggcg ttcgttgcgg ctgccgcggg
    12721 cgcccgggtg gccaagcacg gggggcgggc cgtctcgtcg aagtcgggaa gcgccgacgt
    12781 gctcgagggc ctcggggtga acctcgctct cacgcccgag caggaggcgc tgtgcatccg
    12841 cgaagtgggc gtggggttca tgttcgcgcc cagccaccat ccggccatga agcacgcggc
    12901 ccccgtgcgc aaggaactgg gcatgcgcac catcctcaac atcctgggcc cgctcaccaa
    12961 tccggccggc gcgccgaacc aggtgatggg cgtgttccac acggatctcg tggggatcca
    13021 ggctcgcgtg ctcaagatgc tggggagccg gcacgtgctt accttccacg gccaggatgg
    13081 actcgacgag atcaccatca ccgggccgac gaacgtggcc gagctcaagc atgatttcat
    13141 caccgaatac gtggtcgagc cgcggcagtt cgggctggac aacgcgccca tcgaggccat
    13201 ccaggtagcc gggccggagg agtcgcgggc gcgcgtgctg gccgtcctcg caggcgaggc
    13261 gggaccctct cgcgacatcg tgcttctcaa tgccgcggcg gccctttacg tttcgggcgt
    13321 cgctgcgagc ctctgggacg gcgtcgcgct tgctcgcgaa gcgatcgctt ccggcggcgc
    13381 caaggcaaag ctggaccatc tggttgcctt cactcagacc ttcgccgcgt cgggctcgtg
    13441 agcgacatcc tgcagaagat ccttgccacc aagcgccgcg aggtggaagc cgcgcggatc
    13501 gcggtgccgc tgccggaact cgagcgacgt gcgcgcgctg cacaatcgcc acgcggattc
    13561 accgccgcat tgcaggcgcg catcgcggcg ggaaagccgg cggtgatcgc ggagatcaag
    13621 cgggcgagcc cgagcagggg gctcatccgt gccgacttcg atccggcgcg cattgcgatt
    13681 tcctatgcgg ccaatggggc agcgtgcctg tccgtcctta ccgaccggga gtatttcggc
    13741 gggtcgcgcg aggacctcga agcggcccgg gccgcctgtg ccttgccggt gatccgcaag
    13801 gatttcatca tcgatgccta ccaggtcatc gagtcgcggg cgtggggggc ggattgcata
    13861 ctgttgatcg tggatgccgt atccgattcg tctctcgacc aactcgaggc cctggcgatt
    13921 tcgctgggaa tggatgtgct cgtcgagtcc cacgatgcgg gccagttgga gcgggcgatc
    13981 cgcctgagaa cgcccctgct gggcgtgaac aatcgcgacc tgcgcaattt cgaaatccgc
    14041 ctcgaaacga gcctggaact cgcgaatcgg atcccggccg ggaaaattct ggtcgcagag
    14101 agcggaatcg cgtccccggc ggacgtcgag cggttacgca atggaaaagt gagcgcctac
    14161 ttggtcggca gcgcattcat ggctgcggcg gatccggggg ccgagctttc gcggctgttt
    14221 tcggggctgt gattccggcg ttgcaacaaa gcaacaggcg agggggtttg ttgcgcattt
    14281 cccacccccc ccttgagaga agggcgcggc tttggcatta tgctagcggc ttctcgataa
    14341 atatctggga gaagtgccaa gaatcggggc gaagatcccc aagcggttcc caagcgcttt
    14401 agtgaattcg gcctcacttc aactaaggag attctgaatg aacaagaaac tgatggctct
    14461 ggcagtggct ggcgcttgcg tcgcacctac cgccatggcc cagaccgcca acccggtgac
    14521 cctgtacggg cgcgtatacg tgaatattca gtcggtggag gcgaagggcg gtccggctgc
    14581 gtcgaacgct tccaacagga tgcaggtgag cgacgactcc tcgctgctcg gggtgcgtgg
    14641 cacggaggac atcggcggtg gcaacaaggt cttcttccaa ctcgagaccg cgttcaagcc
    14701 cgactcgaac gccgctgtgt ccaacacgtt cgcgcagcgc aatagcgctg tcggccttca
    14761 gggcggttgg ggtagcctcc tggccggccg gtgggattct ccgtacaagg ttctgaccta
    14821 cggtgccgac atcttcggtg acctgaacct tggcggcatc acggcggcac tgcacgatcg
    14881 cggcaatttc gacgtccgcc agcagaacgt gatccagtac tggacgccca actttgcggg
    14941 attcacggtg aagctcgccg ggaccgccaa cgagggcaag accgcttcgt tgaacccgag
    15001 gcagttcgca ggcaacctgg tgtggtcgag gggcccgatc gcgctgtcct acgcctacga
    15061 ggaacacaag gatgtgagcg cgacaatccg gaaggagacc ggcaacgagg gcgtcgtcga
    15121 attcaagttc ggcgctttca aggtttccgg catctacgag gaaatcaaga aggacggaac
    15181 gaccaagcag aagaactacc tggccagcct cctttggact gcgggcaagc accaggttgg
    15241 ctaccagttc tcgaactcga aagacggtgg cgcgaacggg gcggagaccc agcctgattg
    15301 cgacgtgaat gccgttggct atttctacaa ctggtccaag cgctcgcagt tcgttgcgac
    15361 ctatgtgaag gtcgacaaca atgccgcgag tgtttgcaag ttcggctcca acgatgtcgg
    15421 tggcgccggg gcagaccggg aaggttttgg cgtcggcatc aagcacgtgt tctgatcggc
    15481 tccgcgagag tcgaacgcag catcgagggg cgcggcgcaa gccgcgccct ttttcacgtc
    15541 tagtcatcgg acgcttgcat gatcacagcc ctcgaccgcg tgctcatcgt tgcggacaac
    15601 gcattgcgga ccctcaccgg atccgttaca ccgtctcgcg cagcacccgg ggaaaaggac
    15661 gcgaatcccg cgctgcccga ggagcgtcgc gatgtcgcgg ggctcatgcg cgtcgatcac
    15721 gccggcgaag tctgcgcaca agcgctctat gcggggcagt cgctggtggc gcgtgacggc
    15781 cgcgtcaggc gggctctgga gcgcgccggc gccgaggagc gtgaccacct cgcctggtgc
    15841 gctcagcggc tgcgggagct cgattcgcgc cccagcgtgc tttcccctat ctggtacgca
    15901 gggtccttcg cgctgggaat cgtttcgggc gccgccgggg atcggtggag catggcgttc
    15961 ctggtcgaga cggagcggca ggtcgaaaag cacatcgacg gccatatcga gcgcctgccc
    16021 ccgggcgacg cccgaagccg tgcgatcctg gagcagatgc gcgaagacga gatcggacat
    16081 gcgaacacgg gccgttcact cggcgccggg gaacttcccc tgcccgtccg gagcgccatg
    16141 cgcctcgtgg cgaagctgat gacggtgacg gcctaccgcg tctagccgat cccgcgcgaa
    16201 ggcatcaggc ttcgcggatc tcgtagtcgg aggtgatctc cgccgtcctc gcgagcatga
    16261 tcgttgcaga gcagtatttg tccttcgaca gcttgatcgc ccgctcgacc tgcgcgggat
    16321 tgagtcctcg cccggttacg acatagtgca ggtggatgcg cgtgaagact ttcgggtctt
    16381 ccgcggcacg atcggcctcg agttccacca cgcagtcgac gagatcctgg cgggctttcc
    16441 cgaggatatg gacaacgtcg atcgcgctgc acccgccggc gcccatcagg accatctcca
    16501 tggggcgcgc gcccaggtcc cggccgccca catcgggcgc ggcgtccatc accaccgaat
    16561 gcccgctgcc gctctcggcg acgaatgtca tgccctcgac cagcttcacg cgcgctttca
    16621 taccgcctcc cggagcaggt gtgcctcgag ccaggcattc acgaggctcc atgcgacggc
    16681 cagcagtgcg gggccgacga agatgccgat gaacccgaat gcgacggcgc cgccgaacac
    16741 gccgagaacg acgagcagca tcgacagtcc ccccgcgcgg ctcatgagga tcggtttcac
    16801 gaaattgtcc accgagctga tgacaagggt gccatagatc accatgaaca cggcccaccc
    16861 tgtctcgccc tgaaagtaga gccacgcggc cgcgccgccc cagatgatca cgggccccat
    16921 cggcaccagg gaaaggacga atgtgagcgc ggccagcacc atggccgccg gcacgccggc
    16981 gacgaggaag cccaggagcg acaccgcggc ctgggcgagc gcggtgccga tcagcccgta
    17041 aaccacgccc ttgattgcgt tctgggccgt cgcaaggact gaagcgccgc gctcgccgcc
    17101 cgccaggcgg gccatgcctg tcctgatcat gcgggccacg acctcgccgt cccggtagaa
    17161 gaagaacgcc acgaagatcg ccaggaggat ctgcaggaca ccctcgcccg ccgacgcgcc
    17221 ggcggcaaac agcacatttt tcgccggctc ggccaaccgc ttgccgaggg cgaccatctc
    17281 ctcgcggcta ccgaccagca ctttccagta gtcgtcgatc catcctccga caaacgggat
    17341 gcgggtgagc cagccgggca gctcgatcgg ccctcgatcg aggaagccgc ggatcaggtc
    17401 gatcgcatcg gccgaatggg taatgaggct gtaggcggcc aacgccacgg ggagcgcgat
    17461 ggcgaggagc atgcccagcg cgaacaacag cgcggccagg gtgcttcgcc cgcccacgcg
    17521 ctccttgatc cagcgaaagg cgggccacgt agacaggcac atcacggccg cgaaaaagag
    17581 cgcggcgagg aacgggcgga tgacgaggaa gcacccggcc acgaggaggg cgatgccggc
    17641 tacccgggca tactgttcga agcggctgga gtccatgggc ggggctgggg ggaggtagcg
    17701 aattctatcc gacgggcgga aacgcgtccc cgccgacccc ggcccatgcc cggattgaca
    17761 caagggcctg atttgcttaa aatttcgagt tcgcatccct aatcaccaaa acaaggggcc
    17821 caaagtattt ccggcgcccg ccggaagcac ccaggggcac cataaccgca aggatcacca
    17881 tgaagacctt ttccgccaag ccgcgggaag tcaggcgcga ctggtacgtc gtggacgcca
    17941 ccgacaaggt gctggggcgt ctcgccgtag aggtttcgac ccggcttcgc ggcaagcaca
    18001 agcccgagtt caccccgcac gtcgacaccg gcgactacat cgtcgtggtc aacgtggaga
    18061 agctgcgcgt cacgggcgcc aagtcgagcg acaagatgta ttaccgccac tcgacctacc
    18121 cgggcggcat ctacgagacg acgttcaaca agcttcaggc gcgacatccc gaccgggtcc
    18181 tcaagctcgc cgtgaagggc atgctgccga aggggcgcct gggctacgcg atgatcaaga
    18241 agctcaagat ctacgccggc cccgcgcatc cgcacacgtc ccagcagccc aaagccctcg
    18301 agatctaagg agcgacgacc atgatcggca agtacaatta cggcaccgga cggcgcaaga
    18361 acgcggtggc ccgtgtcttc atcaagtccg gccagggcaa gatcgtcgtc aacgagaagc
    18421 cgctcgacga gtacttctcg cgcgagacgg gccgaatgat cgtgcgccag ccgctcgagc
    18481 tcacgaacca cgcccagacg ttcgacatca aggtgagcgt ctttggcggc ggcgaatccg
    18541 gacaggcggg cgcagttcgc cacggcatca cgcgtgccct catcgagtac gatgcgacgc
    18601 tcaagccggc cctttcgaag gccggtctcg tgacccgcga cgcgcgcgag gtcgagcgca
    18661 agaaggtcgg tttccacaag gcgcggcgcc gcaagcagta ctcgaaacgc taaggcggga
    18721 gagcgctgtc aggcatcgct cgaagacgat caagccgccc cgcaaggcgg ctttttcgtt
    18781 ccgggatcgc gcggccgccc gtggcggcca acggacgagg tgtcgataga tgatcaggat
    18841 tggcgtcgtc ggaggcacgg gatacacggg ggtggaactc ctgaggctgc tggccaccca
    18901 ccccgatgtc gagatcgtgg cgatcacgtc gaggggcgag gccggcacgc cagtcgcgcg
    18961 catctttccg tcgctgcgca agcacgtcga tattgcattc agcgacccgg cggccacgga
    19021 cctgaccgct tgtgactgcg ttttcttcgc cacacccaat ggcgtagcga tgggtcaggt
    19081 ccggccgctg ctcgatgccg gggtgaaagt gatcgacctt tccgcggact tccggctgaa
    19141 ggacatcgcc acgtgggagc gctggtacag gatgaagcat gccgcccccg acctcgtctc
    19201 cgaggccgct tacggcctgc cggaaatcaa ccgggacaag atccgtggcg ctcgaatcgt
    19261 ggccaacccc ggttgctacg cgacagcggt ccagctggcg ctcctgcccc tcgtcgagac
    19321 cgacttcgtg gaccgcgccc acttcattgc ggacgccaag tccggcgtga gcggtgcggg
    19381 gaggaaagcc gaggtggatc tcctgttttc ggaggcatcc gacaacttca aggcctatgc
    19441 ggtcggtggc caccgccatc atccggaaat cgtgcagagc ctgcggcagg cgggtgggaa
    19501 cgaggttggc ctcgctttcg ttccgcacct ggtgccgatg attcgcggaa tcttcgcgac
    19561 cgtctacgcg cgcgtgacgg gcgaggcgga cttccagcag ctcttcgaaa ggcgatatgc
    19621 cgacgaggcg ttcgtggacg tcctgccgcc cggcacccag ccggaaacgc gctgggcgcg
    19681 agggtcgaac gtgtgccgca tcgcggtgca ccggccaatg gacggtgaca cgctcgtggt
    19741 tctcgccgtc gaggacaatc tcgtaaaagg cgcggcaggc caggccgtgc agaacatgaa
    19801 catcatgttc gggcttccag aggggcgtgg cctcgagcag gtggcgctgc tgccgtgaga
    19861 agtctcaccc ggcgcctgcg cagcaacttc ggcatccgct cgtcgcggat gacgatccgc
    19921 tcgcacctgg cgtggtactg gcgctggctg ctgaacgtcc tgatgatggc ggccgtcgcg
    19981 gggatcgtct ggtggctcgt cgagaacagc taccgcatca ccgggttcaa tcgcgaggag
    20041 acgaagcagc agatcgccgc gttcggggag gaaaacgccc ggctgaagcg cgaactcgaa
    20101 gccgcgcgcg tcgtcgtcgc ggagcgcgac cggcagtcgg ccatcgacaa ggccgcgcaa
    20161 atggagctcg cgcggaacgt cgcgcagttg caggacgaga acgccacgct gaaggaggat
    20221 ctcggtttcc tgcgcaacat catgtcgtcg ggcacgactc ccgagggcct gggaatcgcc
    20281 aatctgaagg tggagcagga cgggcagccc aatgagtacc gctacaggat gctgctcacc
    20341 cagggaggtc agcgaaagca ggacttcaag ggcaaggttc aggtgcttgc ccgtgtggcc
    20401 cagggtggcg cggagagcac cctcaccttt ccggagcctt ccgcaggcga ccgcgtgggg
    20461 gacgtggaat tccgctttta ccaaaagctc gacgggcggt tccggatccc cacgggggcg
    20521 gtcctcagat cctgcgaagt ccggattctt gcgcttcccg gaggacaggt taaactctcc
    20581 agaagcgtca acgtccaata ggggaatgtc atgttcggca agggcaacaa gccaagtccc
    20641 atcgacagcc tgatcggcgc cgggacggtc atcgaaggca acgtcacttt cgtgggaggg
    20701 ctgcgcatcg acgggcaggt gaagggcagc gtgaaggcca ccggcagcaa accgggcacc
    20761 ctcgtccttt cggagcttgc caaggtcgag ggcgagatcg acgtggcgca cgtggtcgtc
    20821 aacggcaccg tcgcgggccc cgtccgtgcg accgaatacg tcgaac
//
LOCUS       DASUEV010000195         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_631278, whole genome shotgun sequence.
ACCESSION   DASUEV010000195 DASUEV010000000
VERSION     DASUEV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631278"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /gene="gcvPB"
                     /locus_tag="VFV55_06325"
     CDS             <1..1522
                     /gene="gcvPB"
                     /locus_tag="VFV55_06325"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX4943951.1"
                     /translation="PDDLDAVAEGIPAALARTSAFLAHPVFNRYHAETEMMRYLRRLA
                     DKDIALDRSMIPLGSCTMTLNAVSEMIPVTWPEFANLHPFAPAEQAEGYRELVAGLEK
                     MLVECTGYTAVSMQPNAGSQGEYAGLLVIKAWHESRGEGHRTVCLIPSSAHGTNPASA
                     HMAGMQVVVVACDESGNVDLADLRAKAAQHARNLAAIMVTYPSTHGVFEAGITELCDI
                     VHAHGGQVYVDGANMNALVGLAAPGTFGGDVSHLNLHKTFCIPHGGGGPGVGPIAVAA
                     HLAKFLPGHRYLPSAAQATGPVSAAPFGSASILPISWMYVAMMGGDGLKAATEMAILN
                     ANYVARRLGPHYPVLYTGPGGLVAHECILDLRPLKETSGISVDDVAKRLMDYGFHAPT
                     MSFPVPGTLMVEPTESESKAELDRFCEAMIAIREEIRAVEEGRLDREDNPLKHAPHTA
                     AAVLADDWKHAYPRELAAYPLESLRDNKYWPPVGRADNVYGDRNLFCACVPMDGFA"
     gene            complement(1746..>2101)
                     /locus_tag="VFV55_06330"
     CDS             complement(1746..>2101)
                     /locus_tag="VFV55_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943952.1"
                     /translation="AGARGRAKGADWHFLTTRSTRALVPILDGFGQDLRVDSDSRAAP
                     GTEEFTHTLQVFLIDPRGMVREIYSTAYLMPAMIVNDIRTLALEPPAPRGRRTPPARG
                     TDCAGHASLPVAVSR"
BASE COUNT          348 a          715 c          699 g          339 t
ORIGIN      
        1 gccggatgac ctcgacgcag tcgcggaggg catccctgcc gcgctcgcgc gcaccagcgc
       61 cttcctcgcg cacccggtgt tcaaccgtta tcacgccgag acggagatga tgcgttacct
      121 gcgccgcctc gcggacaagg acatcgcgct cgaccggtcg atgatcccgc tgggctcgtg
      181 cacgatgacg ctcaatgcgg tgagcgagat gatcccggtg acgtggcccg agttcgccaa
      241 cctccatccc ttcgcgccgg cggagcaggc cgaaggctat cgcgagctag tggcgggact
      301 cgagaagatg ctcgtcgagt gtaccgggta cacggccgtg tcgatgcagc ccaacgcggg
      361 ctcgcagggc gagtacgcgg ggcttctcgt catcaaggcg tggcacgaaa gccgcgggga
      421 agggcaccgc accgtctgcc tcatcccgag ctccgcgcac ggcaccaatc cggcctcggc
      481 gcatatggcc ggcatgcagg tcgtggtggt cgcctgcgac gagagcggca acgtggacct
      541 cgccgacctg cgggcgaagg cggcgcagca cgcgaggaat ctcgccgcga tcatggtgac
      601 ctacccctcg acccacggcg tcttcgaggc gggcatcacg gagctgtgcg atatcgttca
      661 cgcgcacggc gggcaggtgt acgtggacgg cgccaacatg aatgcgctgg tggggctcgc
      721 cgcgccgggc accttcggcg gcgacgtctc gcacctgaac ctgcacaaga cgttctgcat
      781 tccgcacggt ggcggcggcc ccggcgttgg ccccatcgcg gtcgcggcgc acctcgcaaa
      841 gttcctgccg gggcaccgct acctgccatc cgccgcacag gcgaccggcc ccgtttcggc
      901 cgcgcctttc ggcagcgcct cgatcctgcc catctcgtgg atgtacgtcg cgatgatggg
      961 gggcgatggc ctcaaggcgg cgacggaaat ggcgatcctc aatgcgaact acgtggccag
     1021 gcgcctgggg ccccactatc cggtgctcta caccggcccg ggcgggctcg tcgcgcacga
     1081 gtgcatcctc gacctgcgcc cgctgaagga aacctcgggc atctcggtcg acgacgtggc
     1141 caagcgcctc atggactacg gcttccatgc gccgacgatg tccttccccg tgccgggaac
     1201 gctgatggtg gagccgacgg aaagcgagtc gaaggccgag ctggaccgct tctgcgaggc
     1261 gatgatcgcc atccgcgagg aaatccgcgc cgtggaggaa gggcgcctcg accgcgagga
     1321 caatcccctg aagcacgcgc cgcacaccgc ggctgccgtt ctcgcggacg actggaagca
     1381 cgcttacccg cgcgagcttg ccgcctaccc gctcgagtcg ctgcgtgaca acaagtactg
     1441 gccgcccgtc gggcgcgcgg acaacgtgta cggcgaccgc aatctcttct gcgcctgcgt
     1501 gccgatggac ggcttcgcgt agccgcctga gcgcgcgcgc gacccgatgc tgcgaggcga
     1561 ttgtattgct tccccgccac agtgccggag cagggtgtgg ttggtgttgt ttttcggttt
     1621 ttggccagac gaaactgctg ccagcgtcga ctccggcaac gtctcgacat aggttcgatt
     1681 cgtaacgccc gatgcatttt gacctggcca aaaaccgaaa aaaacaccaa ccacaccctg
     1741 ctccttcatc gcgatactgc aaccggcaat gatgcatgcc cggcgcagtc agttccccgg
     1801 gcgggcgggg ttctacggcc tcgcggcgcc ggtggttcca gcgccagcgt ccggatgtcg
     1861 ttcacgatca tcgccggcat gagataggcc gtcgagtaga tctcgcgcac catgccgcgc
     1921 ggatcgatga gaaacacctg gagtgtgtgg gtgaattcct ccgtgcccgg agcggcgcgg
     1981 gaatcggagt ccacgcgaag gtcctgcccg aagccgtcga gtatcgggac gagcgcccgc
     2041 gtcgatctcg tggtgaggaa atgccagtcg gcgcccttcg cgcgtccccg ggcgcccgcg
     2101 c
//
LOCUS       DASUEV010000196         7204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_278911, whole genome shotgun sequence.
ACCESSION   DASUEV010000196 DASUEV010000000
VERSION     DASUEV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278911"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFV55_06335"
     CDS             <1..1142
                     /locus_tag="VFV55_06335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX4943953.1"
                     /translation="ETAVEALRAGAHDFVSKPVNREELVHRLEQIEALRGLREENRAL
                     RKSVGGPGADRFRFSSAAMRDVERLASKVAPTDSTVLITGESGTGKGVLARSIHERSQ
                     RAPRPFIAVNCGAIPETLMESEFFGHTKGAFTGADRMRKGLFLEADGGTLFLDEIGEL
                     PLLMQSKLLHAIDDKSVRPVGAESARRIDLRIIAATNRDLSAMVADGRFREDLYFRMS
                     MFRIHVPPLRERPQDLRDFIRHLLESGTASGRRLTGLDPAAEELLLAYSWPGNVRELE
                     NVLNRASILADGERITLADIPAELSAHTAAGGMSVASGPVGDLRSELRRYEAGLVYRT
                     LAMASGDRRQAAQRLGIGLSSLYRKLEEFEREGLGRETDTRPIDV"
     gene            1188..1853
                     /locus_tag="VFV55_06340"
     CDS             1188..1853
                     /locus_tag="VFV55_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4943954.1"
                     /translation="MQVRHFIIALALAAGLATGLARAGPAEDYTAGDAAYRRGDVRSA
                     IASLRKAADAGHAKAQVLLGSILEASQQDADAARYLALAADQGDAEGAYLLATLYSAG
                     QGVDRDPAKARQLLEKAAAVGHRDSVFAIATAYLGGGLGLADADRASAQALAWIRKAA
                     DGGHLPSLDRLALAYRKGELGLTADAKMAEELEARARSIRGITAKPEPARKRPVVRMP
                     GGG"
     gene            1846..2157
                     /locus_tag="VFV55_06345"
     CDS             1846..2157
                     /locus_tag="VFV55_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4943955.1"
                     /translation="MADPRYAALLLVAGIAAAAPVRAADLVRGSQVYALHCAICHGPA
                     GQGGIPGAPKFNRGERLLQSDMALLESVRRGRNIMPSFAGVLRDREILDVIAYLRTLQ
                     R"
     gene            2154..3329
                     /locus_tag="VFV55_06350"
     CDS             2154..3329
                     /locus_tag="VFV55_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX4943956.1"
                     /translation="MTVLRRRLARACALSCLAAFACAAGAASDAKTAAPAAGGPSATG
                     TAAAPKGQEFTHFRVGSKNVKTLYAEGDVLWVGTSGGVIRYDTKKEDFKLFDVKSGLL
                     SNGIFHVSRFQGRMAFGTYGGGLSLLDEKTGKWENFNVPEGLGDAFVYDMLQASNGDL
                     WIATWSGVNRVRGGALRDRSKWSLHTVESTKGGLINDWVYALAEGKDGIIWMATEGGL
                     VRFQDGKWDNWNHARGLGADYEKVKNDIAYRSDPSKVSQHHAKQKMEMGLEDVDVAYN
                     PNYIVAMAVDRQGVVWCGTWGGGLSRFDGKSWKTWTTAEGLPGNHVSLLHVDRSGVLW
                     VGTNNGLAQMKDGRFKVMTVKDGLFANTVFSMVTTAAGGIWVGSFGGVAHIRRPHLH"
     gene            complement(3418..3864)
                     /gene="smpB"
                     /locus_tag="VFV55_06355"
     CDS             complement(3418..3864)
                     /gene="smpB"
                     /locus_tag="VFV55_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX4943957.1"
                     /translation="MSIVENRKAFHDYFIEERFEAGLVLEGWEVKAIRAGRVQLKEAY
                     VIVRGAEAFIIGMHVSALQTASKHVHPDPTRTRKLLMHANELARLIGKVERSGYTLVP
                     VNLHYAKGLVKADIGLAKGKKQHDKRQAEKERDWQREKQRLMRDKA"
     gene            3963..4427
                     /locus_tag="VFV55_06360"
     CDS             3963..4427
                     /locus_tag="VFV55_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX4943958.1"
                     /translation="MPTVTRKVIVDHAAQAMYDLVDGVESYPQFLPWCSGVEVFGRTP
                     TETRARIDIDFHGIRSHVATRNRNIPPEWIHLELADGPFERLAGHWHIRPLGSEGCAI
                     EFSIDYAFSSRALEAVMGPVFGQIMETIVERFVERAAALRSAGPAAVPAGER"
     gene            4427..4717
                     /locus_tag="VFV55_06365"
     CDS             4427..4717
                     /locus_tag="VFV55_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX4943959.1"
                     /translation="MRILVVVALPEGQEVLAVDLEEGASIAQALAAACLAERHPGLAL
                     DRVGVWGRRREPGHVLREGDRVEVYRPLRADAKAMRRERAGVKLSPRSRNGP"
     gene            complement(4683..6278)
                     /locus_tag="VFV55_06370"
     CDS             complement(4683..6278)
                     /locus_tag="VFV55_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943960.1"
                     /translation="MPNDTLPVYTANLDRALAANTVRDTGARALFLAAVHELGKDAPP
                     FGADRLEGLLRIVERFHALLVHDQRGILRGPSIAEGERDFVLTVQRIYLEAANAFQRF
                     LRHRADWASPPEKADKRFRITGLALDAIHCFAKWGGFLGERNRSIPWKQMHALYGLAE
                     SEGYSQVPFVLHATTPEFKPSVQSLYLRALVLDLLNVGNLTPAQVEIADGWLSSWCED
                     YSLDADYSSRAHLFCVDLASERGLRIVRGNRHGDTVRYVQADSLKAQLEEVQADLRHG
                     RLHGGSGTGAFPVEEHVALLAIIEKLYRSMLASAENRIEERTPLEDREIDVVVGIERI
                     LAKTRRGPETAAASPAGPVAAEMVRISPSGHPLSTIVSVPATAAAGDPDVQTWRVKDL
                     SSKGYGLLVDRGAAEEVMLNGAIGLRNQASGGWMLATVARKLANRQRGEILAGVEVLS
                     YLPLRIELAPAEGGPVIGALYLPGSDAGGRLDSIVIRIADFSATAPYRLSAGGATYRI
                     RLNRIASKGADWIRVRFEIEAKA"
     gene            complement(6288..>7204)
                     /locus_tag="VFV55_06375"
     CDS             complement(6288..>7204)
                     /locus_tag="VFV55_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943961.1"
                     /translation="APQSAAAVPAPQSAAAVPAPPPAAAQATVTTPGSNGAPSWLWGV
                     VALLFVAIAILAWRLRVRKAPVPKSELDEAGILMGGRARDGSAGSTVSKHRLATQSGA
                     SPASDVPDTDPAKLRRRYIEERFPEIAGGTIAPDDPDSVVKAARLFYEDGTPPRAVEL
                     LKFAIEENPAAVKPWLALFEIYRLEGLAQEFAALAGRFREHHQPGDIWRKVQFIGRDI
                     DPRNPLYRDAPFDNLETIGYPATKGRGPVSFDPLAENWLDAPMDFATDAFASRLRAGI
                     LADAGVNEADLVANPMPALRNIEMFTVA"
BASE COUNT         1131 a         2347 c         2581 g         1145 t
ORIGIN      
        1 tggagaccgc cgtggaggcg cttcgggcgg gcgcgcacga cttcgtctcg aagccggtga
       61 accgcgagga gctcgtgcac cgcctggagc agatcgaggc gctgcgcggc cttcgcgagg
      121 aaaatcgcgc gctgcgcaag agcgtcggag gccccggcgc ggatcgcttc cgcttttcgt
      181 ctgccgcgat gcgcgacgtc gagcggctgg caagcaaggt ggccccgacc gacagcaccg
      241 ttctcatcac aggcgagagc ggcaccggca agggcgtgct cgcccgctcg atccacgagc
      301 gatcgcagcg ggcgccaagg cccttcatcg ccgtcaactg cggcgcgatt cccgagactc
      361 tgatggagag cgagttcttc ggccacacca agggggcgtt caccggcgcg gaccggatgc
      421 gcaaggggct cttcctcgag gccgacggcg gcacgctgtt cctggacgag atcggcgagc
      481 tgccgctgct catgcagtcg aagctgctgc atgcgatcga cgacaagagc gtgcgcccgg
      541 tgggcgcgga atccgcgcgc cgcatcgact tgcgcatcat cgccgcgacc aaccgggacc
      601 tttcggcgat ggtggccgat ggcaggttcc gcgaggacct ctatttccgc atgagcatgt
      661 tccgcatcca cgtgccgccg cttcgcgagc ggccgcagga tcttcgcgac ttcatccggc
      721 acctgctcga aagcggcacg gcgagcggcc ggcgcctgac gggcctggac ccggcggccg
      781 aggaactgct cctggcgtat tcgtggccgg gcaatgtccg cgaactcgag aatgtcctca
      841 atcgcgcgag catcctcgcc gacggcgagc ggatcaccct ggccgacatc ccggccgagc
      901 tctccgctca caccgcggcg ggtggcatgt cggttgcttc tgggccggta ggggatctgc
      961 gcagcgagct tcgccgttac gaagcggggc tcgtctaccg gactctcgcc atggcctcgg
     1021 gtgaccggcg ccaggccgcg cagcgcttgg gaatcgggct ttccagcctg tatcgcaagc
     1081 tcgaggagtt cgaacgcgag ggcctgggcc gggagacgga cacccgtccg attgacgtgt
     1141 aggaagcgcc gcaggcggcc ttgccgcgcc ggggcgcggg aaagccgatg caggtacgcc
     1201 acttcatcat cgcccttgct ctcgccgcag gccttgcgac gggcctggcc cgggccggcc
     1261 cggcggaaga ctacacggca ggcgatgccg cctaccggcg gggcgacgtg cgcagcgcga
     1321 tcgcgagcct gcgcaaggca gcggatgcag ggcatgcaaa ggcgcaggtc ctgctcgggt
     1381 cgatcctgga agcctcgcaa caggatgcgg acgcggcccg ctatctcgcg ctggcggccg
     1441 accaggggga cgccgaaggc gcatacctgc tcgcgaccct gtattcggcc gggcagggcg
     1501 tggaccggga cccggcgaag gcccgccagc tgctcgaaaa ggccgccgcc gtcgggcatc
     1561 gggactcggt tttcgcgatc gccacggcgt atctgggcgg aggcctggga ctggccgatg
     1621 cggatcgggc gtccgcgcag gccctggcct ggatccgcaa ggcggcggac gggggacacc
     1681 ttccatcgct cgaccggctt gcgctcgcct atcgcaaggg ggaactgggg ctgaccgcgg
     1741 atgcgaagat ggccgaggaa ctcgaagctc gcgcgcggtc gatccggggc atcacggcga
     1801 agcccgagcc ggcgcgcaag aggccggtcg tgaggatgcc cggcggtggc tgatccccgc
     1861 tacgccgccc tgctgctcgt cgcgggaatc gcggcggccg ctcccgtgcg cgccgccgat
     1921 ctcgtgcgcg gcagccaggt ctacgcactg cactgcgcca tctgccacgg ccccgcgggc
     1981 caggggggaa ttcccggtgc gccgaaattc aatcgcggcg agcggctgct gcaatcggac
     2041 atggcgctgc tcgagtcggt gcgacgcgga aggaacatca tgccttcctt cgccggcgtg
     2101 ctgcgcgacc gcgagatcct cgacgtgatc gcctacctca ggaccctgca gcgatgacag
     2161 tcttgcgaag gcggctcgct cgtgcctgcg ccctgtcgtg cctcgcagcc ttcgcctgcg
     2221 ccgcgggtgc cgcgtccgac gcgaagacgg ctgcgccggc tgccggcggc ccgtcggcaa
     2281 ccggaaccgc agcggcgccg aagggccagg agttcaccca cttcagggtg ggcagcaaga
     2341 acgtgaagac gctgtatgcg gagggggacg tgctctgggt ggggacctcg ggcggggtca
     2401 tccgctacga cacgaagaag gaggacttca agctcttcga cgtgaaatcg ggattgcttt
     2461 ccaacggcat cttccacgtc agccgcttcc agggtcgcat ggccttcgga acctacggcg
     2521 gcgggctctc cctgctcgat gagaagacgg gaaagtggga gaacttcaac gtgccggagg
     2581 gtctgggcga tgccttcgtg tacgacatgc tgcaggcctc caacggcgac ctctggatcg
     2641 ccacgtggtc gggcgtgaac cgggttcgcg gcggtgcgct gcgcgaccgc tcgaagtgga
     2701 gcctgcacac cgtcgagagc acgaagggcg ggcttatcaa cgactgggtc tatgcgctcg
     2761 cggagggcaa ggacggaatc atctggatgg cgacggaagg cgggctcgtc cgattccagg
     2821 atggcaagtg ggacaactgg aatcacgcgc gcggcctggg cgcggactac gagaaggtga
     2881 agaacgacat cgcctaccgg agcgatccct ccaaggtgtc ccagcaccac gcgaagcaga
     2941 agatggaaat ggggctcgag gacgtcgacg tcgcctacaa cccgaactac atcgtcgcca
     3001 tggccgtcga ccggcagggg gtggtctggt gcggcacgtg gggtggcggg ctttcgcgat
     3061 tcgacggcaa gtcatggaag acgtggacca ccgcggaagg gctgccgggc aaccacgttt
     3121 cgctgctgca cgtcgacagg tcgggcgtgc tctgggtcgg cacgaacaac ggtcttgcgc
     3181 agatgaagga cggccggttc aaggtgatga cggtcaagga cggcctcttc gccaacaccg
     3241 tgttctcgat ggtcacgacg gcggcgggcg gcatctgggt gggaagcttc ggcggcgtgg
     3301 cgcacatcag gcggccgcac ctgcactgaa ttcccctccc gatttcggga atcggctgcg
     3361 ccggcttcgc gtttgcggga atgccgggcg ccgccgctgg cggctcccct ggaggactca
     3421 ggccttgtcc cgcatgaggc gctgcttctc gcgctgccag tcgcgctcct tctccgcctg
     3481 ccgcttgtcg tgctgcttct tgcccttggc gaggccgatg tcggccttga cgagcccctt
     3541 ggcgtaatgg aggttgaccg gcacgagcgt gtagccgctg cgctcgacct tgccgatgag
     3601 cctggcgagc tcgtttgcgt gcatcagcag cttgcgggtc cgggtgggat cgggatgcac
     3661 gtgcttcgag gccgtctgca gcgcgctcac gtgcatgccg atgatgaatg cctcggcgcc
     3721 gcgcacgatg acgtaggcct ccttgagttg cacgcggccc gcgcggatcg ccttgacctc
     3781 ccatccctcg agcacgagtc cggcctcgaa gcgctcttcg atgaagtagt cgtggaaggc
     3841 cttgcggttc tcgacaatgc tcatgggtgg gggcggttgc gcggggagcg ggaggggaga
     3901 gggggacttg gcgcgcgcga ggttcgcatt ctaccggtgg cgggccgtaa aatgccgctc
     3961 ccatgcctac agtcactcgc aaggtcatcg tcgaccacgc cgcccaagcg atgtacgacc
     4021 tcgtcgacgg cgtcgagtcc tacccgcagt tcctgccctg gtgcagtggc gtcgaggtct
     4081 tcggccgcac gcccaccgag acccgcgcgc gcatcgacat cgacttccac gggatcagga
     4141 gccacgtcgc cacgcgcaac cgcaacattc cgccggaatg gatccatctc gagctggccg
     4201 acgggccatt cgagcgtctc gcgggtcact ggcacatccg gccgctgggc agcgagggct
     4261 gcgcaatcga gttctcgatc gactatgcat tctccagccg tgcgctcgag gcggtgatgg
     4321 ggccggtgtt cggccagatc atggagacga tcgtcgagcg cttcgtcgag cgtgccgcgg
     4381 cgctgcgaag cgcgggacct gcggccgtgc cggccgggga gcgatgatgc gcatcctcgt
     4441 ggtcgtggcg ctgcccgaag gccaggaagt ccttgcggtc gatctcgagg agggcgcgag
     4501 catcgcccag gcgctcgccg ccgcgtgcct ggccgaacgg catcccggat tggcgctcga
     4561 tcgcgtgggt gtgtgggggc ggcgccgcga gccgggccat gtcctgcgcg agggagaccg
     4621 ggtggaggtc taccggccgt tacgggcgga tgccaaggcg atgcgccgcg aaagagccgg
     4681 cgtcaagctt tcgcctcgat ctcgaaacgg accctgatcc agtcggcgcc cttgctggcg
     4741 atccggttca gccggatgcg gtaggtggcg ccgccggcgc tgaggcgata gggcgcggtt
     4801 gcggagaaat cggcgatgcg gatgacgatc gagtcgagcc tgccgccggc atcggacccc
     4861 ggcaggtaaa gcgccccgat caccgggcct ccttcggcgg gcgccagctc gatgcgtagc
     4921 ggcaggtagg aaagcacctc gacgcccgcg agaatctccc cgcgctggcg attggcgagt
     4981 ttcctcgcca cggtcgcgag catccagccg cccgatgcct ggttgcgcag tcctatggcg
     5041 ccgttgagca tcacctcttc ggcggcgccc cggtccacga gcagcccgta ccccttcgag
     5101 ctcaggtcct tcacccgcca cgtctggacg tcgggatccc ctgcggcggc cgtggcgggc
     5161 accgacacga tcgtggagag cgggtgaccg gaaggactga tccggaccat ttcggcggcc
     5221 accggccctg cgggcgaggc ggcagctgtc tcgggcccgc ggcgggtctt cgcgaggatc
     5281 cgttcgatgc cgaccacgac atcgatttcc cggtcctcca gcggcgttcg ttcctcgatc
     5341 cggttttccg cgctggcgag catcgaccgg tagagcttct cgatgatcgc gaggagcgcc
     5401 acgtgctcct ccaccgggaa tgccccggtg ccgctgccgc cgtgcagcct gccgtggcgc
     5461 aggtccgcct ggacctcctc cagttgcgcc ttcaggctgt cggcctggac gtagcggacc
     5521 gtgtcgccgt ggcggttgcc tctcacgatg cgcagtcccc gctcggaggc gaggtccacg
     5581 caaaaaagat gcgcgcgcga ggaatagtcg gcgtcgagcg aatagtcctc gcaccaggag
     5641 gaaagccagc cgtcggcgat ttcgacctgc gcgggagtga ggttgcccac gttcaacagg
     5701 tcgaggacga gcgcgcgcag gtacagcgac tgcaccgagg gcttgaattc cggcgtcgtg
     5761 gcgtgaagga cgaacggaac ctgcgaatat ccctcggact ccgcaagccc gtagagcgcg
     5821 tgcatctgct tccaggggat gcttctgttg cgctcgccca ggaacccgcc ccacttcgcg
     5881 aagcaatgaa tggcatccag tgcgaggccc gtgatgcgga aacgcttgtc cgccttctcc
     5941 gggggcgatg cccagtctgc gcggtgacgc aggaatcgct ggaacgcgtt ggcggcctcc
     6001 aggtagatgc gctgcacggt gaggacgaag tcgcgctcgc cctccgcaat cgacggaccg
     6061 cgcaggatcc cgcgctggtc gtggacgagc aacgcgtgga agcgctccac gatgcgcagc
     6121 aggccctcga ggcgatccgc gccgaaaggc ggcgcatcct tgccgagttc gtgcacggcg
     6181 gcaaggaaga gtgcgcgcgc tcccgtgtcc cgaaccgtgt tcgcggcaag cgcacggtcg
     6241 aggttcgcgg tgtagacggg cagcgtgtcg ttaggcaagg gcggcaatca ggcgacggtg
     6301 aacatctcga tgttcctgag agccggcatg gggttggcga cgaggtccgc ctcgttcacg
     6361 cctgcatccg cgagaatgcc ggcgcgaagt ctcgacgcga atgcgtcggt ggcgaaatcc
     6421 atgggagcgt cgagccagtt ctcggcgagc gggtcgaagc tcaccgggcc ccggcccttc
     6481 gtcgcgggat agccgatggt ctcgagattg tcgaacgggg cgtcgcgata gaggggattg
     6541 cgcgggtcga tgtcgcgccc gatgaactgc accttccgcc agatatcgcc cggctggtgg
     6601 tgttcgcgga agcgaccggc gagtgccgcg aactcctgcg caaggccctc gaggcggtag
     6661 atttcgaaaa gcgcgagcca gggcttcacg gcggccgggt tctcttcgat cgcgaacttc
     6721 agcaattcga cggcgcgagg cggcgtaccg tcctcgtaga agagcctcgc cgccttcacg
     6781 accgagtcgg gatcgtcggg cgcgatcgtg cccccggcga tttccggaaa gcgctcctcg
     6841 atgtatcggc ggcgcagctt cgccggatcg gtgtcgggaa cgtcggaggc gggactggcg
     6901 ccggattgcg ttgccaggcg atgcttgctc accgtgctcc ctgcagagcc gtcgcgcgcg
     6961 cgcccaccca tgaggatccc ggcttcgtcg agttcggact tgggaaccgg ggccttccgg
     7021 acccgcagcc gccacgcgag gatcgcgatg gccacgaaca ggagcgcgac gacgccccaa
     7081 agccacgatg gtgcgccgtt cgaccctggc gtcgttacgg tggcctgcgc tgcggcgggg
     7141 ggcggggcag gcacggcagc ggccgattgc ggggcaggca cggcagcggc cgattgcggg
     7201 gcag
//
LOCUS       DASUEV010000197         4937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_864986, whole genome shotgun sequence.
ACCESSION   DASUEV010000197 DASUEV010000000
VERSION     DASUEV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864986"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..364)
                     /locus_tag="VFV55_06380"
     CDS             complement(161..364)
                     /locus_tag="VFV55_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX4943962.1"
                     /translation="MKTNVGGIDKILRIVAGIGLLAFAAFGEGTARYWGLVGIVPLAT
                     GLFNFCPFYPLLGISTCSAEKKA"
     gene            complement(452..1129)
                     /locus_tag="VFV55_06385"
     CDS             complement(452..1129)
                     /locus_tag="VFV55_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4943963.1"
                     /translation="MSSLPIDKALDRARSSFPALAGISEYLASRLAAECRAMTVPPGT
                     RLFDERSPCGSFPLVIEGRVKVAKLSPSGREIVLYRVGPGQSCLLTSSCLLSRRDYSA
                     TGIAETEVTLVAIPRDLFDALMTGAPAFRDYVHALFADRIGELMMLVEEVAFRRLDQR
                     LAAHLLAHGPLVVARHQDIAVELGSVREIVSRLLSDFEDRGFVSLGRGRIEVADRQGL
                     EAIATGH"
     gene            complement(1135..2223)
                     /locus_tag="VFV55_06390"
     CDS             complement(1135..2223)
                     /locus_tag="VFV55_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4943964.1"
                     /translation="MPTPPAPAPVTKRTLYDILGVERDGNAIDIGIAYKAKMEALRRT
                     PGTDPNEINLVHEAYHILCMPDERAAYDAKLLTMAEKAAAKEAAQNLDLVLEPGEAES
                     LATWKNKYVLTAGALALAFFAFWLARSPSMPKPATVTEGERRADAAPPAPPAPATPVL
                     NAKSAQELLAELSPSTARITVHDVSGRAVGQGSGVVIGAGTVITNCHVAAAGGSVSVK
                     VGREQFSASVEVADEEYDLCRLSVTGLDSPAVTIGTASSLRTGQKVYAIGSPQGLEFS
                     INEGVVSGMHDLPQGRVIQTTAPVPPGSSGGPLFDAYGRLVGIMTFQHRAGRNLSFAV
                     PADWIAGVRSRSATHPLIESMSKAMAPN"
     gene            2359..3015
                     /locus_tag="VFV55_06395"
     CDS             2359..3015
                     /locus_tag="VFV55_06395"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX4943965.1"
                     /translation="MITLERHLTAWAYQNAWANHRLLKACTALSQAEFTAARTSFFPS
                     IKATLNHIVTVDWMYVDAMERAMAGREPHPDIGKFYEPEQPFEHVADLAPAQAAVDRR
                     LITHCAALEAADLERPVAILRRKGRVEEAMHRLLAHVFEHQIHHRGQVHAMLAGTTVA
                     PPQLDEFFCANEADLRASEFAELGFSEAAIWRGNGEQFGKGVRLLFSLPSCDGNATEK
                     "
     gene            complement(3052..3327)
                     /locus_tag="VFV55_06400"
     CDS             complement(3052..3327)
                     /locus_tag="VFV55_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943966.1"
                     /translation="MKPILKPVAIALSAVAFTFASISLPVQAMSTSHAEKVLLHWIKK
                     DAKKVEKISKPVADAAKNGGDALGEYCKKNTDRCIDVALELAALSLV"
     gene            complement(3379..3909)
                     /locus_tag="VFV55_06405"
     CDS             complement(3379..3909)
                     /locus_tag="VFV55_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943967.1"
                     /translation="MLFDRDGVDVVTTSTARQMVMIKDPGSNERYCRGPDPDSSLTSS
                     GGVTLSLPSSTGSKSVGAQKQEDALSFGGRSPAVLITRELMYRACELSMNINADADKT
                     IAIYQEFVRSIERIVASQRQQGAAPLASTKSSEGGSGGDSSDDASDSSDSDADKDGSD
                     KSPKDGSDKSPKKKDK"
     gene            complement(4147..>4937)
                     /locus_tag="VFV55_06410"
     CDS             complement(4147..>4937)
                     /locus_tag="VFV55_06410"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX4943968.1"
                     /translation="RPADAMTFPSLFISHGAPTLALANNFFTHSWAELGVVLGRPESI
                     LVVSAHWDTPFPAVTAAARLETIHDFSGFPEELYRVRYSPPGATELGGRVTELLAAAG
                     LPPATDPDRGIDHGGWVPLRWMYPDADIPVTQLSVQSARGTRHHLGLGRALAPLRAEG
                     VLILASGGIVHNLGELDWDARAARPFPWAVAFNDWMAAKVEGGAIDELADYRRLAPQS
                     ERAHPTEDHLVPFFVALGAGGLPARRMALGFDMGSLGMDCYAFG"
BASE COUNT          798 a         1580 c         1743 g          816 t
ORIGIN      
        1 ggcgcgccaa tcgccgggtc gagatcctgg tcgagccgat cgtcgccgaa taaggacggg
       61 ccgcccccgg cgcctggggg caagctcgca ggatcgaagc caacaaaaaa ggacgccgcg
      121 gcgtcctttt ttgttccctg agcgggcctt gatttctgga tcaggccttc ttctcggccg
      181 agcaggtgct gatgccgagc agcgggtaga acgggcagaa attgaagagg ccggtagcta
      241 gcgggacgat gccgacgagg ccccagtagc gtgccgtgcc ctcgccgaag gcggcgaagg
      301 cgagaaggcc gatgccggcc acgatgcgaa ggatcttgtc gatgccgccg acgttggttt
      361 tcatgggagg tctccttggg gtgcgttgaa tgacgacccg aaggctacgg gggcggcatc
      421 cccccgtctg tgacccaggt tacacagcgt gtcagtgccc ggtggcgatc gcttccagcc
      481 cctggcggtc cgccacctcg atgcgcccgc gcccgagcga gacgaacccg cggtcctcga
      541 agtcgctgag caggcggctc acgatctcgc gcacgctgcc cagttcgacc gcgatgtcct
      601 ggtgcctcgc cacgaccagc gggccgtggg cgagaaggtg ggcggcgagc cgctggtcca
      661 gccggcggaa ggcaacctcc tcgaccagca tcatcaattc acctatccgg tccgcgaata
      721 gcgcgtgcac gtagtcgcga aaggcgggcg cgcctgtcat cagcgcgtcg aacaggtcgc
      781 gcgggatggc caccagcgtc acttcggtct ccgcgatgcc ggtggcggaa taatcgcgcc
      841 gcgacagcag gcaactcgag gtgagcaggc aactctgccc cgggcccaca cggtagagca
      901 cgatctcgcg gccggaaggc gaaagcttgg ccaccttcac gcgcccctcg atcaccagcg
      961 ggaagcttcc gcaggggctg cgctcgtcga agagcctcgt gcccggcggc acggtcatcg
     1021 cgcggcactc ggcggcgagc cgggaggcga ggtattcgga aatcccggcg agggccggaa
     1081 agctcgaccg ggcgcgatcc agggccttgt cgatcggcaa cgaggacatg gaggtcagtt
     1141 cggcgccatc gccttcgaca tcgattcgat gagggggtgg gtggcgctgc gcgagcgcac
     1201 gccggcgatc cagtccgcgg gcacggcgaa gctgaggttg cgccccgcgc ggtgctggaa
     1261 ggtcatgatg cccacgagcc gcccgtaggc gtcgaagagc gggccgccgg aagagccggg
     1321 cgggacgggc gccgtggtct ggatgacacg gccctgcggc aggtcgtgca tgcccgagac
     1381 gacgccctcg ttgatggaga actccaggcc ctgcggcgag ccgatcgcgt agaccttctg
     1441 gccggtcctg agggagctgg ccgtgccgat ggtcacggcg ggcgagtcga ggccggtgac
     1501 cgagaggcga caaaggtcgt attcctcgtc cgcgacctcg acgctggccg agaattgctc
     1561 gcggcccacc ttcaccgaaa ccgagccgcc tgccgcggcc acgtggcagt tggtgatgac
     1621 cgtgccggcg ccgatgacga cgccgctgcc ctggcccacg gcccggccgg aaacgtcatg
     1681 gaccgtgatg cgcgcggtgc tgggcgaaag ctcggccagg agttcctgcg cgcttttcgc
     1741 gttcaggacg ggagtggcag gagccggcgg cgcaggcggg gccgcgtcgg cgcgccgttc
     1801 gccttcggtg acggtcgccg gcttgggcat cgacggcgat cgcgccagcc agaacgcgaa
     1861 aaatgcgagg gcgagcgcgc cggcggtaag gacgtacttg ttcttccagg tcgcgaggct
     1921 ttccgcttcg ccgggctcga ggacgaggtc gaggttctgc gcggcctcct tcgcagcggc
     1981 cttctcggcc atggtgagaa gcttcgcgtc gtaagccgcg cgctcgtcgg gcatgcaaag
     2041 gatgtggtag gcctcgtgga cgaggttgat ctcgttcggg tccgtgcccg gggttcgcct
     2101 gagcgcctcc atcttcgcct tgtaggcgat gccgatgtcg atggcgtttc cgtccctttc
     2161 cacgccaagg atgtcgtaga gggtgcgttt cgtgacgggt gccggggcgg gcggagtggg
     2221 cattgcctca atgatcgggc ttttcaggca gggcgggcaa tggggtgagc taaagtagct
     2281 tcgtcgttcc tgcccaggag gcatcatgcg ccccgccccg gtatccctgc tcccgcccct
     2341 gcctgcgctc gccacgccgt gatcacgctc gaacggcacc tcaccgcctg ggcctaccag
     2401 aacgcctggg ccaatcatcg cctcctcaag gcgtgcacgg cgctttcgca ggcggaattc
     2461 acggcggcgc gaacgagctt cttcccgtcg ataaaggcca ccctcaacca catcgtgacc
     2521 gtggactgga tgtacgtcga tgcgatggag cgcgccatgg ccggccgcga gccgcatccc
     2581 gatatcggca agttctacga gccggagcag cccttcgagc acgtggccga cctggcgcct
     2641 gcgcaggccg ccgtggaccg gcgcctgatc acgcattgcg ctgcgctgga agccgcggac
     2701 ctcgagcggc cggtcgccat cctgcgccgc aagggacgcg tcgaggaggc catgcaccgg
     2761 ctgctggcgc atgtcttcga gcaccagatc caccatcgcg ggcaggtgca cgccatgctc
     2821 gcgggcacaa ccgtggcgcc gccgcaactc gacgagttct tctgcgccaa cgaggcggac
     2881 ctgcgcgcct cggagttcgc cgagctggga ttttccgagg cggcgatctg gcgcggaaat
     2941 ggggagcaat tcggaaaagg ggtcaggtta cttttcagcc tgccgtcttg cgacggcaat
     3001 gcgacagaaa agtaacctga ccccttttcc gaccccctta tcgcgccgcg ttcagacgag
     3061 cgagagggct gcgagttcga gagccacgtc gatgcaacgg tcggtgttct tcttgcagta
     3121 ttcgccgagc gcgtcgccac cgttcttcgc ggcatcggcc accggcttgc tgatcttctc
     3181 gaccttcttc gcgtccttct tgatccagtg caggagaacc ttctccgcgt ggctggtgga
     3241 catcgcctgg acaggcaggg aaatcgaggc gaacgtgaac gcgacggcgg aaagggcgat
     3301 ggcgacgggc ttgaggatgg gtttcatgtt ttcgggtttc ctgagtgtgt gcttcggggt
     3361 ggagaatgcg ccggtctgct acttgtcctt cttcttcggg gacttgtcgg aaccatcctt
     3421 gggggacttg tcggaaccat ccttgtccgc gtcgctgtcc gatgagtcgg acgcgtcgtc
     3481 ggacgaatcg ccgccggagc ccccttcgga ggactttgtc gaggccagcg gcgcggcgcc
     3541 ctgctgccgc tggctcgcga caattcgttc gatgcttctc acgaattcct ggtagatcgc
     3601 gatcgtcttg tcggcatcgg cgtttatgtt catcgacagc tcgcaggcgc ggtacatcag
     3661 ctcgcgtgtg atcagcaccg ccgggctgcg cccgccgaag ctcagcgcgt cttcctgttt
     3721 ctgcgcgccg acgctcttcg agccggtgga cgagggcagc gacaaagtga caccgccgct
     3781 cgaagttagc gaggaatcgg ggtcgggtcc gcggcaatag cgctcgttcg aaccgggatc
     3841 cttgatcatc accatctgcc gggctgtgga cgtggtgacg acgtcgacgc catcgcggtc
     3901 gaacagcatg tcccgcagcg cgacggggct tcccgaggaa gcgcatcctc cggccagcag
     3961 gaggcaggcg gcgaagcagg cgacgcaggc cgagccggat ccggggcggt tcatggtctt
     4021 ggtctttttc ggggcaatgc acgtacccat gcggccggca aaggattcgc cgggaggcac
     4081 ccgaaaaaac tatcatggcc caagggggct tttcttgatc ccgcgcacga tccgcggaga
     4141 tttaggtcac ccgaacgcgt agcagtccat gccgagggag cccatgtcga agcccagcgc
     4201 catgcgccgc gcaggcaggc cgccggcacc cagggcgacg aagaacggca ccaggtggtc
     4261 ctcggtcggg tgggcgcgct cggactgggg cgcgagcctt cggtagtcgg cgagttcgtc
     4321 gatggcgccg ccctcgacct tcgcggccat ccagtcgttg aacgccacgg cccacggaaa
     4381 gggcctggcc gcgcgcgcgt cccagtccag ttcgcccagg ttgtgcacga tgccccccga
     4441 ggcgaggatc agcacgccct cggcgcgcag gggcgcgagc gcccggccga gccccaggtg
     4501 gtggcgggta ccccgcgccg attgcaccga gagctgcgtg acgggaatgt cggcatcggg
     4561 atacatccag cgcagcggga cccacccgcc gtggtcgata ccccggtccg ggtcggtcgc
     4621 cggcggcagt ccggcggcgg cgagaagctc ggtgacgcgt ccgccgagct ccgtcgctcc
     4681 gggaggcgaa tagcgcacgc gatagagctc ctcggggaag ccggagaaat cgtggatggt
     4741 ctcgaggcgc gcggccgccg tcacggcggg gaagggcgtg tcccagtgcg cggacaccac
     4801 caggatcgat tcaggcctgc cgagcaccac gccgagctcg gcccagctgt gcgtgaagaa
     4861 gttgttggcg agcgcgagcg tcggtgcgcc gtgggagatg aagagggatg gaaatgtcat
     4921 ggcgtctgcg gggcgag
//
LOCUS       DASUEV010000198         7809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1554167, whole genome shotgun sequence.
ACCESSION   DASUEV010000198 DASUEV010000000
VERSION     DASUEV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554167"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(476..1939)
                     /gene="tldD"
                     /locus_tag="VFV55_06415"
     CDS             complement(476..1939)
                     /gene="tldD"
                     /locus_tag="VFV55_06415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX4943969.1"
                     /translation="MLANVQPVPVLSALDLATASLLAPFDIDEAAPERVFALVRAHRA
                     DEADLYFQLSRSEGWMLEEGQVKSGSFSIDLGVGVRVVSGEKTAFAYADDISPVSLEA
                     AAKVTRAIAAQGGDAAAAVPSARVAVALYAPVDPVSSLGDAEKVSLLERLEKRARAAD
                     PRVKQVIGRLSGEYEVVFVARSDGVRAADVRPLVNLSITVIVEQDGRREQGYCGGGGR
                     FGYAHFTDERLGHMAEMAVNQALVNLEARPAPAGPMTVVLGPGWPGILLHEAIGHGLE
                     GDFNRKGTSAFSGRIGEKVGARGVTVVDDGTLVDRRGSLSIDDEGEPTRRNVLIEDGV
                     LCGYLQDRLNARLMGVPATGNGRRESYAHMPMPRMTNTYMVGGDMDPAEILGAVERGL
                     YAVNFGGGEVDITSGKFVFSAAEAWWVENGKLQYPVKGATLIGNGPDALTRITMIGNN
                     MALDPGIGTCGKEGQSVPVGVGQPTIRIEGLTVGGTG"
     gene            complement(1987..2802)
                     /locus_tag="VFV55_06420"
     CDS             complement(1987..2802)
                     /locus_tag="VFV55_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX4943970.1"
                     /translation="MFRVAAVQMASGPHVQANLQETARLIELAAATGARIIALPEYFC
                     IMGMKDTDKVAAREKDGEGPIQDFLSAEAKRHKVWIVGGSVPLEATSPDKVRNACLVY
                     DGEGRRVARYDKIHLFGFEMGQEKYTEERTIEPGAAVVTVDSPWGRLGISVCYDLRFP
                     ELYRAMGEVDFIFIPSAFTETTGKAHWDVLVRARAIENQCYVVAPAQGGYHVNGRETH
                     GHTMIVDPWGVVLDRLPRGSGVVVAGVNPTYQAKIRRSLPALRHRTLGRQGTG"
     gene            complement(2865..6767)
                     /locus_tag="VFV55_06425"
     CDS             complement(2865..6767)
                     /locus_tag="VFV55_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEX4943971.1"
                     /translation="MHPRRQPAGSDFSEDSIKAISRSVMRAAAALAGAALVAFLATVL
                     ALRYVVFPQIDSYRDDIAQSISRASGMAVSIVDVDAGWHGLRPILTLTGVRVADRHGK
                     AFFELERAEITLSWWTLLAGDLRFNDIDLYSPQLRLRRGMDGLIYLADKPLNAPVSGE
                     DGALAAWLLDQPRLAIHDATLVWQDEFAGAPELELRQVEIAVRKAGRRHQAALYATPP
                     AAIAARLDLRADLLLARKGEGWQASGSLYGETGRADLERLRAHVPIPDALRTAVGGVR
                     AWVDFEPGQVRDVTADLNLRDVRAQLAADALPLDLETLSGRAYFRLQEGGYAAGTRDL
                     AFRTREGLSAREGDFSLSIGRTARGPARGEVRANGVDLKIAAALLAYLPVPRETKAQA
                     NRYAPRGRLLDTSLVWTGESLAEASAFRLKSRFEDVGVNPVEGFPGASGLTGTLEGDE
                     RGGKLRLASRHATFEAASIFRAPLAFDTLDARATWTRDAKGLEVRIDEAHLANADAEM
                     TVAGTYRTLPGSAEKSPGEVDLAGRVERAHARAVANYLPNGIAVTRDWLDRAILAGEL
                     SRGRFELKGDLWHFPYRDAKRGHFLVEAALDEGRLQYHPAWPSVDRVRGTIRFENARM
                     EITAQDAGIYASKARSVRAVIADLGAGPPVLELDGDIETTGLDSVRFLRETPLVEGPG
                     AFTNAVSIDGPARLKLKLTWPLWGEDPFHLNGEYAFSGATASVGRSLVLTGVRGPLAF
                     TEKSVLAPELTGTMFGQPARLRLATQPDGSVLTQLDGRMGAAALGAFIPEGFARRMAG
                     ATEWKARVVSGALGTELRIESTLKGFAIGLPEPFAKRADESRALAVTIRRLGAADEET
                     VAMLDGGVHARIGRKESGGAVLWNAALKFGAPVTTEPVREGLWLYGNVAHFDLDAWRD
                     ALASREGTVAGEAQSALELRGLDVRFARLRYTGRDFERMTAQLQREAGQWRGTLSSPA
                     VEGEVTFDPRGKGRIAARLKHFGLEAETSGAAGPEPAPPAEQDELPVLDIVAERFEFH
                     GIWLGRLELHAKPDGRDWRIDRLDITNDHARLASSGVWRRTATGSLTQLDLNLETSNL
                     HALLEQFGYGEFVTRGEAKLEGQLVWPGYPYEFAPGVLSGRFNVQASKGQFAKVETGA
                     GKLLGLISLQSIPRRMSFDFRDIFSEGFAFDRISGETKLARGVLLTKDFEVVGPSAFV
                     SMAGEVSLPMETQNLSLKVVPEVGEGIALAATVLGTPVMGLTTLLLQKLLRNPFGKVV
                     SYEYLVTGSWDNPSVTRIGAAPPPRETPKAAPPLPRQP"
     gene            6823..>7809
                     /locus_tag="VFV55_06430"
     CDS             6823..>7809
                     /locus_tag="VFV55_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEX4943972.1"
                     /translation="MIDRISPESFPAAFERTLRLSPFARRIAAARPPLAQALADSGAA
                     ALSRAKLDAAVAAVPMGDPAALAAALRELRARVMLTLIHRDLAGLASLDEVVSTVTQL
                     AEVSIGAAVRAAHAEQAALCGEPARGEQLLVVGMGKLGGGELNVSSDVDLVFLHAAEG
                     ETDGARPLSHHEFFTRVGKRAIGLLSDETGDGYVFRVDMRLRPFGDSGPLVSSFASLE
                     NYFIGQARPWERYAWLKGRVLNGPGEEVEEIVRPFVYRRYLDYGLLESLRDLHGRIQA
                     EANRRGRSGDIKVGAGGIRECEFAVQLFQLVRGGRDAGLRLTSTRAAFAAVAE"
BASE COUNT         1137 a         2732 c         2725 g         1215 t
ORIGIN      
        1 gcggccagcg gcgcctcgca gagcggacag ggagtcccga cgtcgatcat ggcagcttca
       61 cgtgtgaggc gacggaattg gcgatctgtt ttcgcggcgc atggagtgag gtcaggcggg
      121 accagggtgg tcaaatgcca atctgttttc gcggcgcatg gagtgaggtc aggtcggacc
      181 gatgccacga agggttcagg tgggttgggt tcttttttga ttttttggcc ggatgaacgt
      241 gctgccaatc gtgacgtcgg tagcgcttcg acatcgtttc gtccggcgat gtccgacgca
      301 attcaacccg gccaaaaaat caaaaaaaac ccaacccacc tgaacccgat cgacactatg
      361 gtcagcccgg aaaatcactc gcctggtcag cccggaaaat cactcgcctg gtcagccgcg
      421 aatatcgatc gccgaaagcg tcccttcgat cagccgcgaa tatccctcgc cccgatcacc
      481 ccgtcccgcc gacggtgagc ccctcgatgc ggatcgtcgg ctggcccacg cccaccggga
      541 cgctctggcc ctccttgccg caggtgccga tgcccgggtc gagcgccatg ttgttgccga
      601 tcatcgttat gcgcgtgagc gcgtccgggc cattgccgat gagtgtcgcg cccttcacgg
      661 gatactggag cttgccgttc tcgacccacc acgcctcggc cgcggagaac acgaacttgc
      721 cgctggtgat gtccacctcg ccgcccccga aattgaccgc atagagcccg cgctcgacgg
      781 cgccgaggat ttcggccggg tccatgtcgc cgcccaccat gtacgtgttg gtcatgcgcg
      841 gcatcggcat gtgcgcgtag ctttcgcggc ggccgtttcc ggtggcgggc acgcccatca
      901 gccgcgcgtt cagtcggtcc tgcaggtagc cgcacagcac gccgtcctcg atgagcacgt
      961 tgcgccgggt gggttcgccc tcgtcgtcga tggagagcga accgcggcga tctaccaggg
     1021 tgccgtcgtc gaccacggtg acgccacggg cgcccacctt ctcgccgatg cgcccgctga
     1081 acgccgacgt gcccttgcgg ttgaagtcgc cctcgagccc atggccgatg gcctcgtgca
     1141 ggaggatgcc cggccagccg gggccgagca ccaccgtcat ggggccggcc ggggcgggac
     1201 gcgcctcgag gttcaccagc gcctggttca cggccatttc ggccatgtgg ccgaggcgct
     1261 cgtcggtgaa gtgggcgtag ccgaatcgcc cgccgccgcc gcaatagccc tgctcgcgcc
     1321 ggccgtcctg ttccacgatc acggtgatcg agaggttcac cagcgggcgc acgtcggcgg
     1381 cgcgcacgcc gtcggagcgc gccacgaaaa ccacctcgta ttcgcccgaa aggcggccga
     1441 tcacctgctt cacgcgcgga tcggcggcgc gggcgcgttt ctccagccgc tcgaggaggc
     1501 tcaccttctc ggcgtcgccg agggaggaga cggggtccac cggtgcatac agggcaactg
     1561 caacccgcgc gctcggcacg gcggcggcgg cgtcgccacc ctgcgcggcg atggcgcgcg
     1621 tgaccttcgc cgcggcttcg agggaaacgg gcgagatgtc gtcggcgtag gcgaaggcgg
     1681 tcttctcccc ggagacgacg cgcacgccca cgcccaggtc gatcgagaag ctgccgctct
     1741 tcacctggcc ttcctcgagc atccagccct cggaccgcga gagctggaag tagaggtcgg
     1801 cctcgtcggc gcgatgcgcg cgcacgaggg cgaagacgcg ctccggggcg gcctcgtcga
     1861 tgtcgaaggg ggccagcagg gacgccgtcg cgaggtccag ggcggataga acggggacag
     1921 gttggacgtt tgcaagcatg gtgaagtttg atccgttttg ccggttgtca ggggacaggt
     1981 tgcgcttcag cctgtccctt gccgcccgag ggtgcgatgc cgcagcgcag gcaggctgcg
     2041 ccggatcttg gcctggtagg tggggttcac gccggcgacg acgaccccgg agccgcgcgg
     2101 cagccggtcg agcaccacgc cccacgggtc cacgatcatg gtgtggccgt gggtctcgcg
     2161 cccgttcacg tggtagccac cctgcgccgg cgccaccacg tagcactggt tctcgatggc
     2221 gcgggcgcgg acgagcacgt cccagtgcgc cttgccggtc gtctcggtga aggccgaggg
     2281 gatgaagatg aaatccactt cgcccatggc gcgatagagc tccgggaagc gcaggtcgta
     2341 gcacaccgaa atgccgagcc tgccccaggg cgaatccacc gtgaccacgg ccgcgccggg
     2401 ttcgatcgtg cgctcctccg tgtacttctc ctggcccatc tcgaagccga agagatggat
     2461 cttgtcgtag cgcgccacgc gcctgccctc gccgtcgtag accaggcagg cgttgcggac
     2521 cttgtcgggg ctcgtcgcct cgagcggcac ggacccgccc acgatccaca ccttgtgccg
     2581 cttcgcctcg gccgagagga agtcctggat ggggccttcc ccgtccttct cccgcgccgc
     2641 gaccttgtcc gtgtccttca tgcccatgat gcagaagtac tcgggcagcg cgatgatgcg
     2701 cgccccggtg gcggcggcca gctcgatcag gcgcgcggtc tcctgcaggt tcgcctggac
     2761 atgcggcccc gacgccatct gcacggcggc gacgcggaac atgctctgcg tcgtgatcag
     2821 ggtctcgcgg agcgcttccg gcgtcgtgga gtttttcgtg atgatcatgg ctggcggggg
     2881 agcggcggcg cggctttcgg cgtttccctc ggcgggggcg ccgcaccgat ccgggtgacc
     2941 gacggattat cccatgaccc cgtgacgagg tactcgtagg acacgacctt gccgaaggga
     3001 ttgcgcagca gtttctgcag caggagggtg gtgaggccca tcaccggcgt gccgaggacg
     3061 gtcgccgcga gggctattcc ctcccccacc tccggcacca ccttgaggct caggttctgc
     3121 gtctccatcg gcagcgaaac ctcgcccgcc atgctcacga aggcggaggg gcccaccacc
     3181 tcgaaatcct tcgtcagcag gacgccccgc gcgagcttcg tctccccgga tatccggtcg
     3241 aaggcgaaac cctcgctgaa gatgtcgcgg aagtcgaagc tcatgcgccg cggaatcgac
     3301 tgcagcgaga tgaggccgag cagcttgccc gccccggtct cgaccttcgc gaactggccc
     3361 ttggaggcct gcacgttgaa ccgtcccgag agcacgcccg gcgcgaactc gtacggatac
     3421 cccggccaga cgagctggcc ctcgagcttg gcctcgccgc gggtgacgaa ctctccgtag
     3481 ccgaactgct cgaggagggc gtgcaggttg ctcgtctcga gattcaggtc cagttgcgtg
     3541 agggaaccgg tcgccgtccg ccgccacacg ccgctggagg ccaggcgcgc atggtcgttg
     3601 gtgatgtcga ggcggtcgat gcgccagtcg cggccatcgg gcttcgcgtg cagctccagc
     3661 cggcccagcc atatgccgtg gaattcgaag cgctcggcca cgatgtccag gaccggcagt
     3721 tcgtcctgct cggcgggcgg cgcgggctcg gggcctgccg cgccggaggt ctcggcctcg
     3781 agcccgaagt gcttcaggcg agccgcgatg cgccccttgc cccggggatc gaaggtgacc
     3841 tcgccctcga ccgccgggct ggagagcgtg ccgcgccatt gccccgcctc gcgctgcagt
     3901 tgcgccgtca tccgctcgaa gtcccgcccg gtgtagcgaa gcctcgcgaa gcgcacgtcg
     3961 aggccgcgca gttccagtgc ggactgcgcc tcgccagcca cggtgccctc ccgggaagcc
     4021 agcgcgtcgc gccacgcatc gaggtcgaag tgcgcgacgt tgccgtagag ccagaggccc
     4081 tcgcgcaccg gctccgtggt gaccggggcg ccgaacttga gcgcggcgtt ccacagcacg
     4141 gcaccgccgg actccttccg gccgattcgc gcatggacgc cgccgtcgag cattgccacg
     4201 gtctcctcgt cggccgcacc cagccgccgg atcgtcaccg cgagcgcgcg cgactcgtcc
     4261 gcccgcttgg cgaagggctc gggcagcccg atggcgaaac ccttgagcgt cgactcgatg
     4321 cgcagctccg tgcccagtgc gccggagacg acgcgggcct tccactcggt ggcgcccgcc
     4381 atgcgcctgg cgaatccttc cggaatgaag gcgccgagcg ccgcggcgcc catgcgcccg
     4441 tcgagctgcg tgagcaccga gccgtcgggc tgcgtcgcga ggcgcagcct ggcgggttgc
     4501 ccgaacatcg tgccggtgag ttccggtgcg agcacgctct tctcggtgaa ggcgagcggg
     4561 ccgcgcacgc cggtgagaac gaggctccgg ccaacgctcg cggtggcgcc cgagaatgcg
     4621 tactcgccgt tcaggtggaa cggatcctcg ccccagagcg gccaggtgag cttcagcttc
     4681 agcctcgccg ggccgtcgat cgagaccgca ttcgtgaacg cgccggggcc ctcgacgagc
     4741 ggcgtctcgc gcaggaaacg cacgctgtcc aagcccgtcg tctcgatgtc gccgtcgagc
     4801 tcgagaacgg gcggcccggc gccgaggtcg gcaatcaccg ctcgcacgct tctcgccttg
     4861 ctggcgtaga tgccggcatc ctgcgcggtg atttccatgc gcgcgttctc gaagcggatc
     4921 gtgcccctca cgcggtccac cgagggccac gccgggtgat actgcaggcg tccctcgtcg
     4981 agggccgcct cgacgaggaa gtgcccgcgc ttcgcgtcgc ggtacggaaa atgccacagg
     5041 tcccccttca gttcgaagcg cccacggctc aactcgccgg cgaggatggc gcgatcgagc
     5101 cagtcgcgcg tgaccgcgat gccgttggga aggtagttcg cgacggcccg cgcgtgcgcc
     5161 cgctcgaccc gcccggcgag gtcgacctcc cccggcgact tttccgcgga cccgggaagc
     5221 gtccggtagg ttccggcgac cgtcatttcc gcgtccgcgt tggccagatg cgcctcgtcg
     5281 atgcgcacct cgagcccctt ggcgtcgcgc gtccacgtgg cgcgggcatc cagcgtgtcg
     5341 aaggcgaggg gcgcgcggaa tatgctcgcg gcctcgaacg tggcatggcg cgaagcgagg
     5401 cgaagcttgc cgccgcgctc gtccccctcc agcgtgccgg tgaggcccga ggcgccggga
     5461 aacccttcca cgggattcac ccccacgtcc tcgaagcgcg acttcaggcg gaaggcgctc
     5521 gcctcggcga gcgactctcc cgtccacacg agcgaggtat cgaggagccg gccgcgcggc
     5581 gcgtagcgat tggcctgtgc cttggtttcg cgcggcaccg gcaggtaggc gaggagggcg
     5641 gcggcgatct tcaggtcgac gccgttcgcg cgcacctcgc cgcgtgccgg cccgcgggcc
     5701 gttcgcccga tggacaacga gaaatcccct tcccgggcgg agagcccttc gcgcgtccgg
     5761 aaggcgaggt cgcgcgtgcc ggccgcgtag cccccctcct gcaagcggaa ataggcgcgc
     5821 cccgaaagcg tctccaggtc caggggcagc gcgtcggcgg cgagttgcgc acgcacgtcg
     5881 cgcaggttca ggtcggcggt gacgtcacgg acctgccccg gttcgaagtc gacccaggcc
     5941 cggacacccc ccacggcggt gcgcagcgcg tcggggatcg gaacatgggc acgcagccgc
     6001 tccaggtcgg cccgccccgt ctcgccataa agacttcccg aagcctgcca tccctcgccc
     6061 ttgcgcgcga gcagcaggtc ggcacggagg tcgagccttg ccgcgatggc ggccggcgga
     6121 gtggcgtaga gggcggcctg gtgccggcgc ccggccttgc gcaccgcgat ttccacctgg
     6181 cgcaactcca gttccggggc gcccgcgaac tcgtcctgcc agaccagggt ggcatcgtgg
     6241 atggcgaggc ggggctggtc gagaagccag gcggcgagtg ccccgtcctc tccggagacc
     6301 ggcgcgttga gcggcttgtc ggccaggtag atgaggccgt ccatgccgcg gcgaagccgc
     6361 aactgcgggc tgtagaggtc gatgtcgttg aagcgcaggt cgcccgccag cagcgtccac
     6421 cacgaaagcg tgatctccgc gcgctccagt tcgaagaacg ccttgccgtg acggtcggcc
     6481 acgcgcacgc cggtcagggt gagaatgggg cgcagcccgt gccagccggc atcgacgtcg
     6541 acgatggaca ccgccatccc ggaggcgcgc gagatggatt gcgcgatgtc gtcgcggtaa
     6601 ctgtcgatct gcggaaagac gacgtaacga agcgccagca ccgtggcgag gaaggcgacg
     6661 agcgccgcgc cagccagggc tgccgcggcg cgcatgacgg aacgggaaat ggccttgatg
     6721 ctgtcctcgg agaaatcgga tcccgcgggc tgccggcggg ggtgcaacgg tggcgggcgc
     6781 gttttcgcgg tattgtagcg gcagggctgc gcccccctcc tgatgatcga ccgcatttcc
     6841 cccgaatcgt ttcctgccgc cttcgagcgg acccttcgcc tctctccctt tgcgaggcgg
     6901 attgcggccg cgcgcccgcc gctggcgcaa gccctggcgg acagcggcgc ggcggcgctc
     6961 tcgcgcgcca agctcgacgc cgcggtggcg gccgtcccga tgggcgaccc ggccgcgctc
     7021 gccgcggcgc tgcgcgagtt gcgcgcgcgc gtcatgctca ccctgatcca ccgcgacctg
     7081 gccgggctgg cgagcctcga cgaggtcgtt tccaccgtga cccagctcgc ggaggtctcc
     7141 atcggcgcgg ccgtgcgggc ggcgcacgcc gagcaggctg ccctgtgcgg cgagccggcg
     7201 cgcggcgagc agctgctggt ggtgggcatg ggcaagctcg gcggcggaga gctcaacgtc
     7261 tcctccgacg tggacctggt cttcctgcac gcggccgagg gcgagacgga cggcgcgcgg
     7321 ccgctttcgc accacgagtt cttcacccgc gtcggcaagc gcgccatcgg cctcctgtcg
     7381 gacgagaccg gcgacggcta cgtgttccgt gtcgacatgc gcctgcggcc gttcggcgac
     7441 tcggggccgc tggtgagctc ctttgcctcc ctcgagaact acttcatcgg ccaggcgcgt
     7501 ccctgggagc gctatgcctg gctcaagggc cgggtgctga acggcccggg cgaggaggtc
     7561 gaggagatcg tccggccctt tgtctaccgg cgctacctgg attacgggct gctcgaatcc
     7621 ctgcgtgacc tgcacgggcg catccaggcg gaagccaacc gccgcgggcg ctccggcgac
     7681 atcaaggtgg gcgccggcgg catccgcgag tgcgaattcg ccgtgcagct cttccagctc
     7741 gtgcgcggcg ggcgcgacgc ggggctgcgg ctcacctcga cacgcgcagc cttcgcggcc
     7801 gtggccgaa
//
LOCUS       DASUEV010000199         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1187559, whole genome shotgun sequence.
ACCESSION   DASUEV010000199 DASUEV010000000
VERSION     DASUEV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187559"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1768
                     /gene="alkA"
                     /locus_tag="VFV55_06435"
     CDS             311..1768
                     /gene="alkA"
                     /locus_tag="VFV55_06435"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF007641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HEX4943973.1"
                     /translation="MFLDDDQCYRALRAHDARFDGRFFVGVGTTGIYCRPVCTAKTPL
                     ERNCRFFPSAAAAEANGFRPCLRCRPELAPGYASVDANRRLAQAAAGLIEDGRLEDAS
                     FTELAATLGVTDRHLRRVFRQEFGVSPVDYAQTQRLLLAKRLLTDTAFPVLDVAMASG
                     FASLRRFNDLFRTRYRMTPGELRRTAPVRADTDRLAFDLAYRPPYDWPAMLAFLGRRA
                     IAGVESADSRRYRRTVRLQRAGKVRAGWIEVTPSPRKSALRVTASASLAGVVPALLAR
                     VKPLFDLACRPDEIAAALGPLAEATPGLRLPGAIDGFEVAVRAILGQQVTVVAATTIA
                     GRFAKAFGEPIDTPHEGLDRLFPPAALIAATEPSDIASLGIIAARARAIVALAREVDS
                     GALRLDPAAPVDDTLAALAAIPGVGPWTAQYIAMRALAWPDAFPHPDVAVLKAMGETN
                     GRRALARAEAWRPWRSYAVLHLWKSLERTSKEKRK"
     gene            1765..2259
                     /locus_tag="VFV55_06440"
     CDS             1765..2259
                     /locus_tag="VFV55_06440"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX4943974.1"
                     /translation="MTRYARLETPLGTMAAIAEDGKLVALDFEDAKYASAIEPDWIED
                     PAAAPLRDCAAQLAEYFKGERGRFDLPLAARGTPFQQRVWREIAKVPFGETITYAELA
                     ARAGSPGSARAAGAATGRNPIAIVVPCHRIVGANGSLTGYAGGLPRKERLLTHEGALE
                     GAAA"
     gene            2256..3152
                     /locus_tag="VFV55_06445"
     CDS             2256..3152
                     /locus_tag="VFV55_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4943975.1"
                     /translation="MSAADIARLVTLAALWGASYLFMRLSVPHLGAAWVVEGRTLAGG
                     AVLAVFIAATGRAFAIPRHWRGYAVVGVVGVAIPFWLIGTAVKTIDASTAAILNATSP
                     IFSAIIASVWIRERLTVEKIAGIALSITGIAVLVGWTPRPMSAAELLACAMCLSACAC
                     YGFTSVFTNVNLKEAPSSAISASSCLVAAAAMAPFTPWTTAASPAPASAWLSVLALGI
                     LCTGLAFILYYRLIADLGPVRAVMVTLLIPVFGIFWGVVFLGEPVTPGRLSGCAIVLA
                     GCALALGLLRLPFRSSVGLRGE"
     gene            3401..>4156
                     /locus_tag="VFV55_06450"
     CDS             3401..>4156
                     /locus_tag="VFV55_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX4943976.1"
                     /translation="MPNRLAQETSPYLRQHAENPVDWFPWGEEALRAARESGKPILLS
                     VGYSACHWCHVMAHESFEDPEVARVMNDLFVNVKVDREERPDIDQVYQVAQQMLTQRG
                     GGWPLTMFLTADQVPFFGGTYFPKEPRYGLPGFVDLMRRVRAFHDEKRGDIDVQSAEL
                     VAALARTQPRVGARPSGFSDVPLRNAIAFHESLFDAARGGFGTAPKFPHPDTIETCLR
                     HYAASSASSSLDMATTTLAKMAEGGIFDQLGGGF"
BASE COUNT          604 a         1494 c         1366 g          692 t
ORIGIN      
        1 accgtcgcga tgggagagcg cagcggcccc atctctcaat gggtaaaaag tcggggacag
       61 acgtgtttgg ctggccccga ctttttggga cccattcgac ggtctcgctt gcgtgcccag
      121 gacgccgatt aacttgcccc atccccggcc ctcaccctga ttcgccgcgt gcatcaggca
      181 ccgaggcagt gtcgccgcct atgaacgcgg tccacgtcgc tggaattcgg tgatggcgac
      241 gatcgcgggg accgaatccc atgtccgaaa acggcgagcg ctcggggtcg cgggcgcgga
      301 taatgccgcc atgttcctcg acgacgacca gtgctaccgc gcccttcgcg ctcacgacgc
      361 gcgcttcgac gggcgcttct tcgtgggcgt gggcacgacg ggcatctact gccgccccgt
      421 gtgcaccgcg aagacgccgc tcgagcgcaa ctgccgcttc tttcccagcg cggcggcggc
      481 cgaggcgaac ggcttccgcc cgtgccttcg ctgccggccc gagctcgcgc ccggctacgc
      541 ctcggtcgat gcaaaccggc gcctcgcgca ggccgcggcg ggattgatcg aagacggccg
      601 cctcgaggac gcgagcttca ccgagctggc cgcaacgctg ggcgtcacgg accgccacct
      661 gcgccgcgtc ttccggcagg aattcggcgt ctcgcccgtg gactacgcgc agacgcagag
      721 gctgctgctg gccaagcgac tcctcaccga cactgcgttc ccggtgctcg acgtggcgat
      781 ggcgagcggg ttcgcgagcc tgcgccgctt caacgacctg tttcgcaccc gctaccgcat
      841 gacgccgggc gaactgcgca ggacggctcc ggtccgtgcc gacaccgacc ggcttgcctt
      901 cgacctcgcc tatcgcccgc cctatgactg gcccgcgatg ctcgccttcc tcgggcgccg
      961 cgccattgcg ggtgtcgagt cggcagactc gcgccgctac cgccggacgg tgcgcctgca
     1021 gcgcgcggga aaggtgcgcg ccggctggat cgaggtgacg ccctcgccgc gcaagtcggc
     1081 attgcgcgtt accgccagcg catctctcgc gggggtggtg ccggcgctcc tggcgcgcgt
     1141 gaagccgctt ttcgacctcg cctgccggcc cgacgagatc gccgccgcgc tgggcccgct
     1201 cgccgaggcg accccgggcc tgcgactgcc gggggccatc gacggcttcg aagtggcggt
     1261 gcgtgcaatc ctcgggcagc aggtgaccgt ggtggccgcc acgacgatcg cgggccggtt
     1321 cgcgaaggcg ttcggcgagc cgatcgacac gccgcacgag ggactggatc gcctctttcc
     1381 cccggctgct ctgatcgcgg cgacggaacc ttcggacatc gcttcgctgg gcatcatcgc
     1441 cgcgcgcgcc cgcgccatcg tcgccctcgc gcgcgaggtc gattccggcg cgcttcgcct
     1501 cgaccccgcc gcgcccgtcg atgacacgct ggccgccctt gcggccatac ccggcgtggg
     1561 cccgtggacc gcgcaataca tcgcgatgcg cgcgctcgcg tggccggacg cattcccgca
     1621 ccccgacgtc gcggtgctga aggccatggg agagacgaac ggccggcgag cgctcgcgcg
     1681 ggccgaagcc tggcgcccgt ggcgctcgta tgccgtgctc cacctgtgga aatcgcttga
     1741 acggacatcg aaggagaaaa ggaaatgacc cgttacgctc gcctcgagac acccctggga
     1801 accatggccg ccatcgccga agacggcaag ctcgtcgccc tcgacttcga ggatgcgaag
     1861 tacgcatccg cgatcgagcc agactggatc gaggaccccg cggccgcgcc gcttcgcgac
     1921 tgcgcggcgc agctcgccga atacttcaag ggcgaacgcg gacgcttcga cctgccgctc
     1981 gccgcgcgcg gcacgccctt ccagcagcgc gtgtggcgcg agatcgccaa ggtgcctttc
     2041 ggggaaacga tcacctacgc ggagctcgcc gcgcgcgccg gttccccggg atccgcacgc
     2101 gccgccggcg ccgcgacggg gcgcaacccg atcgccatcg tcgtgccgtg ccaccgcatc
     2161 gtgggcgcga acggctcgct caccggctat gccggcggat tgccgcgcaa ggaaaggctc
     2221 ctcacccacg aaggagccct cgagggagcc gccgcttgag tgcggccgat atcgcaaggc
     2281 tcgtgacgct tgcagctctc tggggcgcgt cctacctgtt catgcgcctt tcggtgccgc
     2341 acctcggcgc cgcctgggtc gtcgaggggc gcacgctggc cggcggagcg gtgctcgccg
     2401 tcttcatcgc cgccacgggg cgcgccttcg cgataccgcg ccactggcgc ggctatgccg
     2461 tggtcggcgt ggtgggcgtg gcgattccct tctggctcat cggcacggcg gtgaagacga
     2521 tcgacgcgtc cacggccgcg atcctcaacg ccacctcgcc gatcttttcc gcgatcatcg
     2581 catcggtgtg gatccgcgag cgcctcacgg tggagaagat cgcgggcatc gcgctttcca
     2641 tcaccggcat cgcagtgctc gtgggatgga cgccccggcc gatgagcgcg gccgagctcc
     2701 tcgcctgcgc gatgtgcctc tcggcgtgcg cctgctacgg attcacgagc gtgttcacca
     2761 acgtcaacct gaaggaagcg ccgtcgtccg ccatctccgc gtcgagctgc ctcgttgccg
     2821 cggccgcgat ggcgcctttc acgccgtgga ccacggccgc gagcccggca ccggcgtcgg
     2881 catggctctc ggtcctggcg ctgggcatcc tgtgcacggg actggcgttc atcctctact
     2941 atcgcctgat cgccgacctc ggcccggtgc gggcggtgat ggtgaccctg ctcattcccg
     3001 tcttcggcat cttctggggc gtggtattcc tcggcgaacc cgtcacgccg gggcgccttt
     3061 ccggctgcgc catcgtcctt gcaggctgcg ctctcgctct cggcctgctg cgccttcctt
     3121 tccgcagttc cgtagggtta cggggggagt gatgcgggcg taaacctttg gaacacggat
     3181 gaacacggat ggacacggat aaaagcggat aaaggcaatt gggtcgagag ggtcttatgc
     3241 cgcgaattga cgacatcgcc acgctggaat acggaccgtt ctcgacccgc cttcacctgc
     3301 atttatccgc ttttatccgt gtccatccgt gttcatccgt gttccaaagg tttacgcccg
     3361 catcactcgc gctccccctt ttcggtagca tcactctcaa atgcccaaca gactcgcgca
     3421 ggaaaccagc ccctacctcc ggcagcacgc ggaaaatccc gtcgactggt tcccgtgggg
     3481 cgaggaagcc ttgcgcgccg ccagggaaag cggaaaaccc atcctccttt ccgtcggcta
     3541 ttccgcctgc cactggtgcc acgtgatggc gcacgagtcc ttcgaggatc ccgaggtcgc
     3601 cagggtgatg aacgacctct tcgtgaacgt gaaggtcgac cgcgaggagc gccccgacat
     3661 cgaccaggtc taccaggtcg cgcagcagat gctcacgcag cgcggcggcg gctggccgct
     3721 gacgatgttc ctcacggcgg accaggtgcc gttcttcggg ggaacctact ttccgaagga
     3781 gccgcgctac ggcctgcccg gtttcgtgga cctcatgcgc agggtgcgcg cgtttcacga
     3841 cgagaagcgt ggcgacatcg acgtgcagag cgcggagctg gtcgcggcgc ttgcgcgcac
     3901 gcagccccgc gtcggcgcgc gcccttccgg cttctcggac gtgccgctgc gcaatgccat
     3961 cgccttccat gagagcctct tcgatgcggc acgagggggc ttcgggacgg cgcccaagtt
     4021 cccgcacccc gacacgatcg agacgtgcct gcgccactac gctgccagca gcgcgtcctc
     4081 gtccctcgac atggccacga cgacgctcgc gaagatggcc gagggcggaa tcttcgacca
     4141 gctgggcggc ggcttc
//
LOCUS       DASUEV010000200         8981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1188628, whole genome shotgun sequence.
ACCESSION   DASUEV010000200 DASUEV010000000
VERSION     DASUEV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188628"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFV55_06455"
     CDS             <1..514
                     /locus_tag="VFV55_06455"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919206.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX4943977.1"
                     /translation="VKSNLSGEFTVVNEHLVRELKKRGLWDSVMISDLKHFDGSIGKI
                     DRIPADLRSLYATAFEVEPRWIVEAGARRQKWIDQAQSLNIYMAGASGKKLDETYKLA
                     WLRGLKTTYYLRTLAATSAEKSTGKGGELNAVPAHGGLGALGDGAGDGAADIAGKFCS
                     IDNPECEACQ"
     gene            691..2307
                     /gene="groL"
                     /locus_tag="VFV55_06460"
     CDS             691..2307
                     /gene="groL"
                     /locus_tag="VFV55_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310604.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX4943978.1"
                     /translation="MAAKQVVFGAEARARIVAGVNLLAEAVRCTLGPKARTVVLERAF
                     GPPVVINSGVIVAREIELPDALENMGAQMAREVASKTSDVAGDGTTTAILLARGLVVE
                     GMKHVAAGMNPMDLKRGIDLAVVEIVAELKRLARPCGTRNAIAQVAAISANNDPVIGE
                     IVANAMEKAGKEGVITVEDGTGLASELEVVEGMQFDRGYLSPYFINTAEKQRVVLEDA
                     YILACDRRISNLAEMLPVLELVARTGKPLLVVAEDVDGEALATLVVNTLRGVLRTCAV
                     KAPGFGDRRKAMLEDLAILTGGRVISGEAGLKLEGTGLADLGHARRIEIDKDDTTIIG
                     GGGEPDRVTARVAEIKQQVDDATSDYDKENLRERAAKLAGGVAVVKVGAATETEMKER
                     KARVEDALHATRAAVEEGVLPGGGVALLRARARLAGLRGANPDQQCGIAIVLRAVEEP
                     LRQIVSNAGDEPSVVLERVLAGGQDFGFNALDGRYGDMVEMGILDPCKVTRTALQNAA
                     SIAGLILTTEAMVAGMADEGNKDLPPMEGM"
     gene            2304..2618
                     /locus_tag="VFV55_06465"
     CDS             2304..2618
                     /locus_tag="VFV55_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4943979.1"
                     /translation="MNRNKSQERPRPGQAVHSTAAELEGLRVVERPDGFWVQPASGGR
                     EIGPYASVAEAIEDQRATDEDELEPGGALAEVEAQLGVSDWIDPDTAAPAEDSVPHIE
                     EH"
     gene            2629..4227
                     /locus_tag="VFV55_06470"
     CDS             2629..4227
                     /locus_tag="VFV55_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4943980.1"
                     /translation="MPDGNEPTKFASFVERLRLPACYPHPAGEVELLETHISWVLLAG
                     EYAYKLKKPVSLGFVDFSTLESRRFYCEEELRLNRRTAPDLYLEVVPITGTPDSPVIG
                     GTGPVIDYAVKMRRFPQEALLDRVAKAGELTAATVDAFARSVASFHGRVARAGRDGAF
                     GAPPEILAEAVDNFAQLELLEGPEDARGSRQALCSWTLGEFNRLEPLFARRKREGFVR
                     ECHGDLHLGNVALLAGEPVAFDAIEFNEAFRWVDVMNEVAFPVMDLFHHGLPRLARRF
                     LDAYLDATGDFDGLRVLRFYTVYRAMVRAKVSCIRAHQAGMEAASREQSARACRGHLD
                     LAGKLARPAATALIVMHGLSGSGKTTVSQALVEELGAIRLRSDVERKRLHGLEAGART
                     HSAPGGGIYTAEADRLTYGRLATLAGQVLDAGYPVVVDAAFLRSAWREMFREFARERN
                     APFVLVACAAPEETLRARIEERERRASDASEAGSAVLARQIATLETLAAGELDAAVIV
                     DSTAPGDQPMQVARRIASRLGVPV"
     gene            complement(4254..4844)
                     /locus_tag="VFV55_06475"
     CDS             complement(4254..4844)
                     /locus_tag="VFV55_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX4943981.1"
                     /translation="MLRNDDVLVIVDVQNDLVSGSLAVPGGAEVIAPLNRYIEAFVRK
                     GLRVVATRAWHPADHRSFRENGGPWPTHCLARSPGAAFAPGLRLPDGSLLVSKATAPD
                     REAGSGFAGTDLDHRLRRLGAKRLFVGGLATDQCVLRTVLDARRLGYGVFVLADAIRA
                     MDLRPGDGDNAEAEMQREGAVFIHFDSLTEETDSLA"
     gene            complement(4853..5509)
                     /locus_tag="VFV55_06480"
     CDS             complement(4853..5509)
                     /locus_tag="VFV55_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX4943982.1"
                     /translation="MVFVDRIDAARRLGKSLAAFKGKNPLVLAIPRGAVPMGRVVADE
                     LGGELDVALVRKLGAPGNPEFAVGAVDEAGWTYIAAYAAEVGASPSYLADETAAQLAA
                     MRTRRAMYTPARSPIDPAGRIVIVMDDGLATGATMIAALHSVRSRHPARLVCAVPVAS
                     PDSLEKVRAHADEVFCLEAPPEFHAVGQFYRDFGQVDDSAVVAALAGRGIPAATLSTG
                     "
     gene            5612..>8981
                     /locus_tag="VFV55_06485"
     CDS             5612..>8981
                     /locus_tag="VFV55_06485"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HEX4943983.1"
                     /translation="MDALIDRATETAGKRLARHVLAAQLQWPALAALCLVPIAAYNYL
                     LFHTVVELVSVIVACSVFVIAWNAYPFSHNRFLLFLGTGLLWVSALDLFHALVYKGMD
                     IVPGFAGANAPTQFWIGTRYLHALVLLAAPLFLVWPLKRWWAMLGNGMAACAVFAWLV
                     SGHFPDAFVEGAGLTPFKIYSEYAIVAILALSLAFLWQRRTALDERIFVLVAASTAFV
                     MAGELAFTKYASVYGQANLAGHLFRIVAYWLIYAALVDRALAQPFRMMARAATTYDAI
                     PDATIVVDKEGVIRQVNGAARVQTGLPGQEAIGRDCHALFHPASVPRDACPACRCIRE
                     GTAAPSLELEDATGGRWVEISLAPVARGEDVTGMVHVSRDITERHNAERDKEVLIRSL
                     NERMKELRCLYAISDLLERKGRDLPEVLAQIAALLPAAFQFPDITVARIACGAVERAT
                     PGFRRTSWRLGAGVTARGEPVGSVEVCYLEERPACDEGPFFDEERQLIDVVAERLGRI
                     IERVRAEEQVARLTRLYATLSQTNQSIIRATDRSALFEEICRIACESGGFASAWISVG
                     GSGADGFSVAASRGLDDADRALLAESAGNPEGHVARVLRTGESWFTNDYANDSATSRW
                     HREETARGIAGCAIVPLRTGGAVAGLFGLYTREAAYFDDAQLALLEEMASDVSFALDN
                     LEHERRRREGERALRESEERYRQVGASVLDGLIVIDDQGKITDWNSAAARIFSYTAAE
                     AVGRDLHELVALSRYRDRYEAGLAHFRSSGQGAAIGKSLELTARRRDGSEFPVELSIA
                     GFQWKGRWNAVGSVRDITDRVLAQKKLRFATAVVERSPTVLFRYRAEEGWPVEYVSEN
                     VRQVGYEAEELLSGAVSLASLIHPDDLGRVTDEMARHCASGAESFAQEYRIVTKGGAV
                     LWVDDQTIVERDPAGTVTHFQGVVTDVTERRRAQQAELEQFRLAEAFFNHSVSAIAIL
                     DRDFNFVRVNDAYARGARKDIGEFAGRNHFEMYPSDAKAIFEEVVRTRRPYETTAREF
                     TYPGQAERGKTYWDWTLVPILDRQGEVEYLVFSLQEVTERKLAEERQRAAALYARSLI
                     EASLDPLITISPEGKVTDVNRMTEEATGVARAELI"
BASE COUNT         1443 a         2983 c         3180 g         1375 t
ORIGIN      
        1 cgtgaagtcg aacctgtcgg gcgagttcac ggtggtgaac gagcacctgg tgcgcgagct
       61 gaagaagcgc ggcctgtggg acagcgtcat gatctcggac ctcaagcact tcgacggttc
      121 catcggcaag atcgaccgca ttccggctga cttgcgcagc ctttatgcga cggccttcga
      181 ggtggagccc cggtggatcg tcgaagccgg cgcgcgccgc cagaagtgga tcgaccaggc
      241 gcagtcgctc aacatctaca tggccggggc ctccggcaag aagctggacg agacctacaa
      301 gctcgcctgg ctgcgcggcc tgaagaccac ctactacctg cgcacgctgg ccgccacctc
      361 cgcggaaaag tccacgggca agggcggcga actcaacgcg gttcccgccc acggcgggct
      421 gggcgccctg ggcgatggcg ccggtgatgg tgccgctgac attgccggca agttctgttc
      481 catcgacaat cccgagtgcg aggcctgcca gtagggcaac cctcggcgcg atccactcgc
      541 gcaggaaggg aatgatgggg ccaggttccc gaacctggcc ccgttttttt cgcgactttc
      601 ctcgggatga gcgccggtgc gccctgttga tgcgggtcaa cttttccccc ctcttgcggc
      661 gtagcttgga atcgtcagga ggcaatttcc atggccgcca agcaggtcgt cttcggagcc
      721 gaggcacggg cgaggatcgt cgcaggcgtg aacctgctgg cggaggccgt gcgctgcacc
      781 ctcgggccga aggcacggac ggtggtgctc gagcgcgcgt tcggcccccc ggtggtcatc
      841 aattccggcg tcatcgtcgc ccgcgagatc gagttgccgg acgcgctcga gaacatgggc
      901 gcccagatgg cgcgcgaggt ggcgagcaag acctccgacg tggccggcga cggcaccacc
      961 accgccatcc tcctggcacg ggggctggtg gtcgagggca tgaagcatgt cgcggcgggc
     1021 atgaacccga tggatctcaa gcgcggcatc gaccttgccg tcgtcgagat cgtcgcggaa
     1081 ctcaagcgac tggccaggcc ctgcggcacg cgaaacgcga tcgcccaggt ggccgccatc
     1141 tccgccaaca acgacccggt gatcggggag atcgtcgcga atgcgatgga gaaggccggc
     1201 aaggagggcg tcataaccgt ggaggacggc accgggctcg cgagcgagct ggaggttgtc
     1261 gagggcatgc agttcgaccg cggctacctg tcgccgtatt tcatcaacac ggcggagaag
     1321 cagcgcgtgg tgctggagga cgcgtacatc ctcgcctgcg accgcaggat ctcgaatctt
     1381 gccgagatgc tgccggtgct ggaactcgtg gccaggaccg gcaagcccct cctcgtcgtg
     1441 gccgaggacg tggacggcga ggcgctcgct accctggtgg tgaacacgct tcgcggcgtg
     1501 ctcaggacct gcgccgtgaa ggcgcccggc ttcggcgacc ggcgaaaggc catgctcgaa
     1561 gacctcgcca tcctcaccgg cggacgcgtc atctccgggg aggcgggcct gaagctcgag
     1621 ggcacgggcc tcgcggacct cgggcatgcg aggcgcatcg agatcgacaa ggacgacacc
     1681 acgatcatcg gcggcggcgg cgaaccggac agggtcacgg ccagggtcgc ggagatcaag
     1741 cagcaggtcg acgatgccac cagcgactat gacaaggaga acctgcgcga gcgggcagcc
     1801 aagctcgcgg gcggcgtggc ggtggtcaag gtgggcgccg ccaccgaaac cgaaatgaag
     1861 gaacgcaagg cgcgcgtcga ggacgcgctg cacgccacgc gcgcggccgt ggaggaaggt
     1921 gtcctgcccg gcggcggcgt ggcgttgctg cgcgcgcgcg ccaggctggc cggcctgcgc
     1981 ggcgcgaacc ccgaccagca gtgcggcatc gcgatcgtgc tgcgcgccgt ggaggagccc
     2041 ctgcggcaga tcgtatccaa cgcgggcgac gaaccttccg tggtcctgga acgggtgctc
     2101 gcgggcggcc aggatttcgg cttcaacgcg ctcgacggcc gatacggcga catggtggaa
     2161 atgggcatac tggatccctg caaggtcacg cgaacggcgt tgcagaacgc ggcctcgatc
     2221 gcggggctca tcctcaccac ggaagccatg gtcgccggga tggcggatga gggaaacaag
     2281 gacctgccac cgatggaggg aatgtgaacc gcaacaagag ccaggagcgg ccccgtcccg
     2341 ggcaagccgt ccacagtacg gccgccgagc tcgagggcct gcgcgtcgtt gagcgcccgg
     2401 acggtttctg ggtgcagccc gcctcgggcg gacgcgagat cggtccctac gccagcgtcg
     2461 ccgaggccat cgaggatcag cgcgccacgg acgaggatga actcgagcca ggcggcgcgc
     2521 tcgccgaagt cgaggcccag ctcggtgtat cggactggat cgaccccgac accgccgcgc
     2581 ccgccgagga cagcgtgccg cacatcgaag agcattgaga gcgcgcgcat gccggacggg
     2641 aacgagccga cgaaattcgc gagcttcgtc gagcggttgc gactgcctgc ctgctatccg
     2701 catcccgcgg gggaagtgga actcctcgaa acgcacatct cgtgggtgct gctggcgggt
     2761 gaatacgcgt acaagctcaa gaagcccgta agcctgggct tcgtcgattt ctccaccctc
     2821 gaatcgcgcc gcttctactg cgaggaggaa ttgcggctca accgccgcac cgcgccggac
     2881 ctctatctcg aggtcgtgcc gattaccggc actcccgatt cacccgtcat tggcggcacc
     2941 ggcccggtca tcgactacgc cgtgaagatg cgtcgcttcc cgcaggaggc gctgctggac
     3001 cgcgtggcga aggcggggga actgaccgcg gcgacggtgg acgcgttcgc tcgttccgtc
     3061 gcctccttcc acgggcgcgt ggcgcgcgcg ggccgcgacg gcgcgttcgg cgcgcccccg
     3121 gagatcctgg ccgaagccgt ggacaacttc gcacagctcg aactcctgga agggccggag
     3181 gacgcgcgcg gatcgcggca ggcgctttgc tcgtggacgc tcggcgagtt caaccgcctc
     3241 gaaccgcttt tcgcgcgccg caagcgcgag ggattcgtgc gcgaatgcca cggcgacctc
     3301 catctgggca acgtggcgct gctcgccggc gagcccgttg ccttcgatgc gatcgagttc
     3361 aacgaggcgt tccgctgggt cgacgtgatg aacgaggtgg ccttcccggt gatggacctc
     3421 tttcaccacg ggctgccgcg cctggcccgc cgcttcctcg acgcctatct cgacgctacg
     3481 ggggacttcg acgggcttcg cgtgctgcgc ttctacaccg tctaccgcgc gatggtgcgc
     3541 gccaaggtgt cgtgcatacg cgcgcaccag gcaggcatgg aagccgccag ccgggagcaa
     3601 tcggcgcgcg catgccgggg ccacctggac cttgccggga aactcgcgcg gcccgccgcg
     3661 accgcgctga tcgtcatgca cggtctctcc ggctcgggca agacgaccgt atcgcaggcg
     3721 ctggtggagg agctgggcgc cattcgcctt cgctccgatg tcgagcgcaa gcgcctgcac
     3781 gggctggaag cgggggcgag gacgcattcg gcccccggcg gcgggatcta cacggcggaa
     3841 gcggaccggc tcacctacgg ccgccttgcc acgctggccg gccaggtcct cgacgcgggc
     3901 tacccggtgg tcgtggacgc ggccttcctg aggagcgcgt ggcgcgagat gttccgggaa
     3961 ttcgcacgcg agcgcaatgc gcccttcgtg ctggtcgcgt gcgcggctcc ggaagagacg
     4021 ttgcgcgcgc gcatcgagga gcgcgaacgc cgcgccagcg atgcctccga ggcgggcagc
     4081 gcggtgctcg cgcgccagat cgccacgctc gagaccctgg ctgccggcga actggacgct
     4141 gccgtgatcg tcgattcgac cgcgccgggg gaccagccga tgcaggtcgc ccggcgcatc
     4201 gcctcgcgac tcggcgttcc cgtctgaagg cggtcgcggc tgcgactcgc ccttcaggcg
     4261 agcgagtcgg tctcctcggt gaggctgtcg aagtggatga acaccgcgcc ttcgcgctgc
     4321 atttcggctt ctgcgttgtc cccgtcgccc gggcggaggt ccatcgcgcg gatggcgtcg
     4381 gccagcacga agaccccgta gccgaggcgg cgcgcgtcga gcacggtgcg cagcacgcac
     4441 tggtccgtgg ccagcccgcc tacgaacagg cgcttcgcgc ccagcctcct gaggcggtgg
     4501 tcgagatcgg tgccggcgaa cccggaaccc gcctcgcggt caggcgccgt cgccttcgag
     4561 acgagcaagg atccgtcggg cagccgcagg ccgggcgcga acgccgcgcc ggggctcctc
     4621 gcgaggcaat gcgtgggcca cgggccgccg ttctcgcgaa aggagcgatg gtccgcggga
     4681 tgccaggcgc gtgtcgcgac gacgcgaagt cccttgcgca cgaacgcctc gatgtagcgg
     4741 ttgagcgggg cgatgacttc ggcgccgccc ggcacggcga ggcttcccga caccaggtcg
     4801 ttctggacgt ccacgatgac cagcacgtcg tcgttccgga gcatcgcgga attcatcccg
     4861 tcgacagcgt tgcggccgga attccccttc ccgcgagcgc agcgacgact gcgctgtcat
     4921 ccacttgtcc gaaatcgcgg tagaactggc ccaccgcgtg gaattccggc ggcgcctcga
     4981 ggcagaagac ctcatcggcg tgcgcacgaa ccttctccag gctgtcgggc gaggccaccg
     5041 gaaccgcgca taccaggcgc gcggggtggc gcgagcgtac cgaatgcagc gccgcgatca
     5101 tcgtcgcgcc cgtggccagg ccgtcgtcca tgacgatgac gatgcggccc gcgggatcga
     5161 tcggtgagcg agccggcgtg tacatcgcgc ggcgcgtgcg catggcggcg agctgcgccg
     5221 cggtctcgtc ggcgagatag gacggggagg cgcccacctc ggccgcgtag gccgcgatgt
     5281 aggtccaccc cgcttcgtcc accgcgccca ctgcgaactc cggattgccg ggcgcgccga
     5341 gcttgcgcac cagcgccacg tccagttcgc cacccagctc gtccgcgacc acacgcccca
     5401 tcggcacggc gccccggggg atcgcgagca cgagcggatt cttccccttg aaggcggcaa
     5461 gcgacttgcc gagccgccgc gcggcatcga tgcggtccac gaacaccatg gatcgaagct
     5521 agtcctcccc gccggtcgtt gcttgaccgg gatcaaccag ccgctccgga cgcaggacaa
     5581 gatgggattc ggcggcaaat cgcgaggatg catggatgcc ctgatcgacc gggcaacgga
     5641 gacagcgggc aagcggctgg ctcgccatgt gctggccgcc cagctgcagt ggccggcact
     5701 cgcggccctg tgcctcgtgc ctatcgcggc ctacaactac ctgctgttcc acacggtggt
     5761 ggagctggtg tccgtgatcg tggcctgcag cgtgttcgtg atcgcgtgga acgcctatcc
     5821 cttttcgcac aaccggttcc tgctgttcct cggcacggga ttgctgtggg tgagcgcgct
     5881 ggacctgttc catgcgctcg tctacaaggg catggacatc gtgccgggct tcgcgggcgc
     5941 gaatgcgccg acgcagttct ggatcggcac ccgctacctg catgccctgg tgcttctcgc
     6001 ggcacccctc ttcctcgtct ggccgttgaa gcgctggtgg gcgatgctgg gcaacggcat
     6061 ggccgcgtgc gccgtgttcg cctggctcgt gtcgggccat tttcccgacg ccttcgtcga
     6121 gggcgccggg ctcacgccgt tcaagatcta cagcgagtac gccatcgtcg cgatcctcgc
     6181 gctgtcgctc gccttcctgt ggcagcgccg cacggcgctg gacgagcgca tcttcgtgct
     6241 ggtcgcggcc tccacggcat tcgtcatggc cggcgagctc gctttcacga agtacgcaag
     6301 cgtctacgga caggcgaacc tcgccgggca cttgttcagg atcgtggcct actggctcat
     6361 ctatgccgcc ctggtcgacc gtgcgctggc gcaaccgttc cggatgatgg cgcgtgccgc
     6421 gaccacgtac gatgcgatcc ccgatgcgac gatcgtcgtg gacaaggagg gcgtgatccg
     6481 ccaggtcaac ggggccgcgc gcgtgcaaac ggggctgccg ggccaggaag ccatcggccg
     6541 cgattgccat gcgctcttcc acccggcgtc ggtgccccgc gacgcgtgcc cggcgtgccg
     6601 ctgcatccgg gaagggacgg cggccccgtc gctggaactc gaggacgcga ccggcggacg
     6661 ctgggtcgaa atctcgctgg cgccggtggc gcggggcgag gacgtgaccg gcatggtgca
     6721 cgtgagccgc gacatcacgg agcggcacaa tgcggagcgg gacaaggagg tcctgatccg
     6781 cagtctcaac gagcggatga aggagctgcg ctgcctttac gccatctccg acctcctcga
     6841 gcgcaagggc cgcgacctgc cggaagtgct ggcccagatc gcggcgctgc tgccggccgc
     6901 cttccagttt cccgacatca ccgttgctcg catcgcttgc ggtgccgtgg agcgcgccac
     6961 gccggggttc cggcgcacgt cgtggcggct cggcgccggc gtgactgcgc ggggcgagcc
     7021 ggtcggcagc gtcgaggtct gctacctgga ggagcggccc gcctgcgacg aaggtccgtt
     7081 cttcgacgag gagcgccagc tcatcgacgt ggttgccgag cgactggggc ggatcatcga
     7141 gcgcgtgcgc gccgaggaac aggtggctcg actcacgcgg ctgtacgcga ccctcagcca
     7201 gaccaaccag tcgatcatcc gcgcgaccga ccggtcggcc ctgttcgagg agatctgccg
     7261 cattgcctgc gagtcgggcg gattcgcctc cgcatggatc agcgtcgggg gcagcggagc
     7321 cgacggattt tccgtggccg catcccgcgg cctggacgac gccgaccggg cgcttctcgc
     7381 ggaatcggcc ggaaatcccg agggccacgt ggcacgggtg ctgcgtacgg gcgagtcgtg
     7441 gttcacgaac gactacgcca acgatagcgc caccagccgc tggcaccgcg aggagactgc
     7501 gcgagggatc gcgggatgcg caatcgttcc gctgcgcacg ggtggggcgg tcgcggggct
     7561 cttcggtctt tacaccaggg aagcggccta cttcgacgac gcgcagcttg cgctgctgga
     7621 ggagatggca tccgacgtct ccttcgcgct ggacaacctg gaacacgagc gccggcgccg
     7681 ggagggcgag cgggcgctgc gcgagagcga ggagcgctac cggcaggtcg gcgcgtccgt
     7741 gctggacggc ctcatcgtga tcgacgatca ggggaagatc accgactgga attcggcggc
     7801 cgcgcgcatc ttctcctaca cggcggcgga agcggtcggc agggatcttc acgaactcgt
     7861 ggccctgtcg cgttaccggg accgctacga ggcgggcctg gcgcatttcc gctcgagcgg
     7921 gcagggggcg gcgatcggca agtcgctcga gttgaccgcc aggcgccgcg acggctccga
     7981 gttccccgtg gaattgtcga tcgcggggtt ccagtggaag ggccggtgga acgccgtggg
     8041 gtcggtgcgc gacatcacgg accgcgtgct cgcgcagaaa aagctgcgtt tcgccacagc
     8101 cgttgtcgaa cgcagcccga cggtgctgtt ccgctatcgc gccgaggagg gctggccggt
     8161 cgagtacgtt tcggaaaatg tccggcaggt cggctacgag gccgaggagc ttctttcggg
     8221 tgccgtttcc cttgcgagcc tgattcatcc ggacgatctc gggcgcgtga ccgatgagat
     8281 ggcgcggcac tgtgccagcg gcgcggagag tttcgcccag gaataccgca tcgtgaccaa
     8341 gggcggtgcg gtcctctggg tggatgacca gacgatcgtc gaacgcgacc ccgcggggac
     8401 cgtcacgcac ttccagggag tcgtcacgga cgtcacggag cgcaggcgcg cgcagcaggc
     8461 ggaactcgag cagttcaggc tcgccgaagc cttcttcaat cacagcgtga gcgccatcgc
     8521 cattctcgat cgcgatttca atttcgtgcg cgtgaacgac gcctatgcgc ggggtgcgag
     8581 gaaggacatc ggcgagtttg cgggacgcaa tcacttcgag atgtatccgt cggacgcgaa
     8641 ggccatcttc gaggaggtgg tgcggaccag gcgcccgtac gaaacgacgg ccagggagtt
     8701 cacctatccc gggcaggccg aacgcggcaa gacatactgg gactggacgc tggtgccgat
     8761 cctcgaccgg cagggcgagg tggagtacct ggtcttttcc ctgcaggaag tcaccgagcg
     8821 caagctcgcg gaagaaaggc agcgtgccgc cgcgctctac gcccgcagcc tcatcgaggc
     8881 gagcctcgat ccactgatca ccatcagccc ggaaggcaag gtcaccgacg tcaaccggat
     8941 gaccgaggag gccaccggag ttgcccgggc ggagctcatc g
//
LOCUS       DASUEV010000201        14635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_865672, whole genome shotgun sequence.
ACCESSION   DASUEV010000201 DASUEV010000000
VERSION     DASUEV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865672"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFV55_06490"
     CDS             <1..205
                     /locus_tag="VFV55_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX4943984.1"
                     /translation="GVVDTNNSLDGIDYIIPGNDDSTRAIRLYARGVADAILEGRSQV
                     ITEMVAPTTGEFVEVEDAEEAKG"
     gene            286..1164
                     /gene="tsf"
                     /locus_tag="VFV55_06495"
     CDS             286..1164
                     /gene="tsf"
                     /locus_tag="VFV55_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX4943985.1"
                     /translation="MAEITASMVKELRELTGLGMMECKRALEESGGDLKKAEELLRIK
                     SGAKASKAAARVASEGAIGMHVSGDGKLGAMVEVNCETDFVARNPDFAAFARSLAELV
                     ATANPADVAALSALAIGGKTVEEARQALVQKIGENITVRRFERVQTAARLAHYVHGVK
                     IGVIVEFEGQDAAGKDVAMHIAFAKPRFLSKDQVAADVLAGERTIIEARARESGKPPE
                     IAAKMVEGGVNKFLGEITLLGQPFVKDDKQTVEKMLAAAGSKVLSYRFLVVGEGIEKK
                     QADFAAEVAAMTKTAA"
     gene            1230..1946
                     /gene="pyrH"
                     /locus_tag="VFV55_06500"
     CDS             1230..1946
                     /gene="pyrH"
                     /locus_tag="VFV55_06500"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX4943986.1"
                     /translation="MTTPAYTRILLKLSGEALMGDDAFGINRAVVERIVGQVKEVAEL
                     GVQVGIVIGGGNIFRGVSQGAAGMDRATADYMGMLATVINALALQDAMNRSGLVARVQ
                     SALNIEPVCEPYIRGKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGREVEAQIVLK
                     ATKVDGVYTADPLKDPKATRYDRITYDEAFVKNLKVMDATALALCRDQRLPINVFSIF
                     KEGALRRVVMGEPEGTLVHS"
     gene            1961..2527
                     /gene="frr"
                     /locus_tag="VFV55_06505"
     CDS             1961..2527
                     /gene="frr"
                     /locus_tag="VFV55_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX4943987.1"
                     /translation="MALNMADIKKQAADRMAKSIETLRLDLAKVRTGRAHAGILDHLR
                     VDYYGTPTPINQVANVTLVDARTIGVQPYEKKMVQAVERAIRDSDLGVNPATSGDMIR
                     VPMPALTEERRRELTKIVKHEGEEAKVAVRNVRRDANTHLKEMLKKHDVPEDEEKRVQ
                     DEVQKLTDKAVGDIDRMVAEKEKELMAV"
     gene            2551..3318
                     /gene="uppS"
                     /locus_tag="VFV55_06510"
     CDS             2551..3318
                     /gene="uppS"
                     /locus_tag="VFV55_06510"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX4943988.1"
                     /translation="MAEIESSTLAIPRTGDVPRHVAIIMDGNGRWAKSRYLPRVAGHR
                     RGVEAVREVVKACIARGVECLTLFAFSSENWRRPKEEVTFLMQLFLKALEQEVEKLHG
                     NGIRFKVIGDLSAFDPVIVEHIRRSEALTAGNDRLKLTVAANYGGRWDILQAANRCRT
                     EHPGEEITEELLSRYLSMPFEPEPDLFIRTGGEERISNFLLWQLAYTELHFTEVLWPD
                     FGAEALDEAFASYRSRERRFGRTSDQVAATLPQPKVG"
     gene            3339..4172
                     /locus_tag="VFV55_06515"
     CDS             3339..4172
                     /locus_tag="VFV55_06515"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX4943989.1"
                     /translation="MLKTRVLTAIALLPLVLGMLFFSGPAAWTAFATAIALVSCWEWS
                     RLCGFGRGSRGLYLLLCAAIALALATASLRGPEHVFANVAQASFVAAACFWIFAVPAW
                     LALRLRPEPWASGVAGWFVLWPMWAALVVLREASPWILLAAAALVWVADIAAYFAGRR
                     FGQRKLAPAISPGKTWEGVAGALVGVLAYGVALDLYAHARPGPLSPLFGSAGGALAVL
                     AMVVLAALSVLGDLFESWMKRGAGRKDSSGLLPGHGGILDRIDALTPTLPVAALLLSF
                     A"
     gene            4169..5371
                     /gene="ispC"
                     /locus_tag="VFV55_06520"
     CDS             4169..5371
                     /gene="ispC"
                     /locus_tag="VFV55_06520"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX4943990.1"
                     /translation="MKRLTILGSTGSIGESTLDVVARHPDRFEVVALAARRGHEKLAA
                     QCERFAPRYAAMADEAAAERLRAALRARGIATEVLAGPDSLERVAALPETDAVMAAVV
                     GAAGLPGTLAAARAGKQVLLANKEALVVSGSLFLAALAEGGGTLLPIDSEHSAVFQSL
                     PRGFTCGRQGEAARAGVRRILLTASGGPFRTTPLERLGNVTPDEACAHPNWVMGRKIS
                     VDSATMMNKGLEVIEAFWLFGAPSEAIEVVIHAQSVIHSMVEYHDGSVIAQLGAPDMR
                     TPIACALAWPERIEAGVAPLDFASLSRLEFGRVDDARFPCVRLAYAALRRGGTAPAIL
                     SAANEVAVEHFLAGSLAFTAIAAVIENVLGSVTVEPLDSLEVAWRADRGAREAARREV
                     SRLGRRAG"
     gene            5392..6762
                     /gene="rseP"
                     /locus_tag="VFV55_06525"
     CDS             5392..6762
                     /gene="rseP"
                     /locus_tag="VFV55_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX4943991.1"
                     /translation="MSGAFVTIASFLVTVGLLVVVHELGHYAVARLAGVKILRFSIGF
                     GRSLWMRRLGKDRTEWVIAAFPLGGYVKMLDSREGEVETAERARAFDLQPVGKRIAIA
                     LAGPAANFLAAFLLYWALFVIGLPGVKPVVGDPPRASPAAAAGLANGDTIRAIGGEPT
                     ATWTDVRWLLLKEAVRGEDVAIEVEGEGGARATRSLALSSLTKEDLDRDFLMKLGLKA
                     FTPHVPATLGRIVPGGAGERAGLREGDHVVAIGGQPVATWGQFTARVRASAGRTLSLE
                     VERSGRRLVVPVVPEATGEGEARIGLLKVEPGAEVRREWERMTTTVRYDPLAAVPRAL
                     ARVWDLSAFSLRMLGKMLLGEVSWKNLSGPITIADYAGQSAQMGWVSFAGFLALVSVS
                     LGVLNLLPIPLLDGGHLVYYSAEILKGSPVSERAMEIGQRVGLAILAGLTFFAFYNDI
                     NRLLTG"
     gene            6767..9064
                     /gene="bamA"
                     /locus_tag="VFV55_06530"
     CDS             6767..9064
                     /gene="bamA"
                     /locus_tag="VFV55_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX4943992.1"
                     /translation="MKNPRFRSLRLAIACALAASLPALAIQPFVVKDIRVEGVQRTEA
                     GTVFSYLPVKVGEQIDDLKAAKAVKALYATGFYSDVRLEADGDVLVVFVQERPAIAGI
                     EIEGAKEFTKDNLKDGLKQAGIAESKIYDKSLLDRAEKELKRQYTSRGFYSSTVRITV
                     TPLERNRVSLRFDIEEGEITRIADINIIGAKDFTERQLLREIQLTTPGWLTWITKDDQ
                     YSKQKLTADLETLRSFYLNRGYLEFAIDSTQVSITPDREKIYITIAITEGPVYRLGDI
                     KFSGDLIVAERELRELLSLRSGDTFSREKIIDSVKRITDRLGNDGYSFANVNPVPDLD
                     RQKRVAGFTFFVDPGRRVYVRRVNIVGNSRTQDAVIRRELRQLESSWYSLEKIARSKE
                     RLQRTSYFSEVNIETPAVAGTADQVDVVVTVTEKNTGSLNFGVGYSQVDKLTVSASVA
                     QANLFGSGNQLAFQVNNGSINRVYSLSFIDPYWTIDGIAAGIDIFRRDVDTSSLVVTS
                     YKTTSSGVGVNFSVPITEYDTVRVGLTAEATKLQLDTAAAPARYLDFVNTYGERTNTL
                     RTNVGYARDTRDSLTYPTRGWLTEFLLEIGIPPGDLKYYRASAQQQFLWTPDRLSWLT
                     FLVNAEFGYADGYGGKPLPFFKNFYAGGVGSVRGFEAASLGPRDLNGDVLGGNRRLIG
                     NLELLFPMPGIKDKSVRLSTFLDAGSVWGSDEKIVGADIRLSTGVAVSWDSPVGPLKF
                     SFSAPIKKKADDKLERFQFQLGRIF"
     gene            9083..9577
                     /locus_tag="VFV55_06535"
     CDS             9083..9577
                     /locus_tag="VFV55_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX4943993.1"
                     /translation="MDWRTGLAASLGAIALGGMAAEVKIGFVNTERVFREAAPAKRAQ
                     QKLEREFSTRNAELAKLEKHGRDLQVELEKDSVTLPEAARREKERQLADVSRNFQRLQ
                     REIREDLNLRRNEELAQVQERATRVINQIAEAEKFDLVVQEAVFASSRIDITDKVIKA
                     LADK"
     gene            9593..10657
                     /gene="lpxD"
                     /locus_tag="VFV55_06540"
     CDS             9593..10657
                     /gene="lpxD"
                     /locus_tag="VFV55_06540"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX4943994.1"
                     /translation="MPPQSPTARDPAFTLQEIVRRLGGEVAGDASVTVNGVAAIDEAG
                     PGEITFLANPRYRSRLAATRAGAVILGPRDRDATAIARIVADNPYAYYARTVALFNPP
                     RVVVPGTHPTACVDPGARVAASAEIGPFAVIAAGARVGERACIGAGSVVGEGASIGDD
                     TILYPRVTVYHACVVGARCILHSGAVIGADGFGMAPDAGRWVKIPQVGRAVLGDDVEV
                     GANTTIDRGALGDTVVEDGVKIDNQVQVAHNCRIGAHTVIAGCVGISGSVVIGRHCLI
                     GGGAGIVGHITLCDGVTVSGMTFITKSITRPGVYSSGMPMMPHADWLRNAAQLRRLDE
                     TARLARGKSAPGEEREDDDD"
     gene            10647..11084
                     /gene="fabZ"
                     /locus_tag="VFV55_06545"
     CDS             10647..11084
                     /gene="fabZ"
                     /locus_tag="VFV55_06545"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX4943995.1"
                     /translation="MTIDEIKAFLPHRYPFLLIDRVVDFEKDLRIVAVKNVTANEPFF
                     QGHFPHFAVMPGVLVVEAMAQAAALLSLRSLGRKNDAKSVYYFVGIDRARFKRPVVPG
                     DQLMIEVEQLRLARGMGKFAAVARVDGSVAAEAELLCTLRPIG"
     gene            11098..11874
                     /gene="lpxA"
                     /locus_tag="VFV55_06550"
     CDS             11098..11874
                     /gene="lpxA"
                     /locus_tag="VFV55_06550"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX4943996.1"
                     /translation="MTAIHPTAIVDPRARLAAGVRVGPYTVIDGDVEAGEGTTFGAHC
                     VVTGHTTLGRDNRIFHFCSIGEACQDKKYGGEPTRLVIGDRNTIREYCSLNRGTVQGG
                     GVTTVGHDNWIMAYSHIAHDCRVGDHTVFANHATLAGHVDIGDHTVLGGFTGVHQFVK
                     VGAHVMAGVSSVVLQDVPPYVTVAGHPCSPHGINSEGLRRRGFTPEALAALKRAYKTL
                     YKSGLALAEARAELERQSLAAPEVKAIVDFLATSTRGIVR"
     gene            11908..13098
                     /gene="lpxB"
                     /locus_tag="VFV55_06555"
     CDS             11908..13098
                     /gene="lpxB"
                     /locus_tag="VFV55_06555"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX4943997.1"
                     /translation="MTRRVAIVAGEASGDMLGASLVRAVRSRFPDTQFYGIAGPRMMA
                     AGASTLVPMEKLSVRGYVEAMRSLPELLRIRSRLAARLVSDRPDLFIGVDAPDFNLGL
                     EARLKRAGIPTVHYVSPSIWAWRAGRLPSIGRAVDRVLTIFPFEAALYEKAGIAATYV
                     GHPLADEIPFVPDRADARAQLRLDEATTAVALLPGSRRGELEAHADLFIDTARRLSAR
                     RPGARFFVPLATRETRDFFESRLYAREAQELDLTILFGHARLALQAADCALVASGTAT
                     LEGALARCPMVITYKVPALTYRLMMRKALLPYVGLPNILAGEFIVPELLQEHATPENL
                     EQALANWLDHKVARARLVERYEAIHAQLRCGNDERVAQALAPWLSNAQETGTLSLEQL
                     AFKG"
     gene            13073..13687
                     /gene="rnhB"
                     /locus_tag="VFV55_06560"
     CDS             13073..13687
                     /gene="rnhB"
                     /locus_tag="VFV55_06560"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX4943998.1"
                     /translation="MNNSPSKGSVPRSCGVDEAGRGPLAGAVFAAAVILDPSRPVPGL
                     ADSKLLSAARREALARVIRRDALAWAIASATVEEIDAINILQASLAAMARAVAALGVD
                     PDEVVVDGLHVPRMRWPSRAIVKGDRLVPAISAASILAKTARDAEMVILDARFPGYGF
                     ARHKGYPTADHLAALRSLGPCAIHRRSFEPVRAVIALCDPAFSR"
     gene            13722..14528
                     /locus_tag="VFV55_06565"
     CDS             13722..14528
                     /locus_tag="VFV55_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX4943999.1"
                     /translation="MKTIASRDNAAYKSLARLCTSAAERRKRGLSVIEGAHLVEACLD
                     AGHPVAQLFVSAAATGSAQATALRARAKAAEMVALSDALFDAVSGVESPAGILAVVPT
                     PEGRAVPADARFCLALEDLQDPGNVGTLLRSAAAAGVEHVLLSPTCAFAWSPKVLRAG
                     QGAHFALNIVEGTDLAAFVASFAGTTIALAGKAKRSLYETDLRGPVAFLVGNEGAGLS
                     AELERSVTIRARIPMPGCAESLNAGVAGSIALFEAVRQRGVTLPKGGGHG"
BASE COUNT         2257 a         5121 c         5053 g         2204 t
ORIGIN      
        1 gggtgtggtc gacaccaaca actcgctcga cggcatcgac tacatcatcc cgggcaacga
       61 cgactccacg cgcgccatcc gcctctacgc ccgcggcgtg gcggacgcga tcctcgaggg
      121 ccgcagccag gtgatcaccg agatggtggc gccgaccacg ggcgagttcg tcgaggtcga
      181 ggacgccgag gaagcgaagg gctgaggcct ttcgcgtgac atttgcgcga accggggggc
      241 gccaagcccc cttttcgcat ccgtaatgcg actggagcaa tgaaaatggc ggaaatcacc
      301 gcgagcatgg tgaaggaact gcgcgagctg accgggctcg gcatgatgga atgcaagagg
      361 gccctcgagg aatcgggcgg cgacctgaag aaggccgagg aactgctgcg catcaagagc
      421 ggcgccaagg catccaaggc ggctgcgcgc gtggcatcgg aaggcgccat cggcatgcac
      481 gtctccggcg acggcaagct cggcgcgatg gtcgaggtga actgcgagac ggacttcgtc
      541 gccaggaacc cggatttcgc cgcattcgcc cggtcgctgg ccgaactggt cgccaccgcc
      601 aacccggccg acgtggccgc tctctcggcg ctcgcgatcg gcggcaagac ggtcgaggag
      661 gcgcgccagg cgctggtgca gaagatcggc gagaacatca ccgtgcgccg cttcgagcgg
      721 gtgcagaccg ccgcgcgcct cgcgcattac gtgcacggcg tgaagatcgg cgtgatcgtg
      781 gagttcgagg gccaggacgc cgcgggcaag gacgtcgcga tgcacatcgc cttcgcaaag
      841 cccaggttcc tctcgaagga ccaggtggcc gccgacgtgc tcgccggcga gcgcaccatc
      901 atcgaggcgc gcgcccggga atccggcaag ccccccgaga tcgcggcgaa gatggtcgag
      961 ggcggcgtca acaagttcct cggcgagatc acgctcctcg gccagccgtt cgtgaaggac
     1021 gacaagcaga cggtcgagaa gatgctcgcc gcggccgggt cgaaggtgct ctcctaccgg
     1081 ttcctcgtcg tcggcgaggg catcgagaag aagcaggccg acttcgccgc cgaagtggcg
     1141 gccatgacga aaaccgcagc ctgacgaacc cggtttgtgt acccggtacg tgtaccgggt
     1201 acatgtaccg ggtacacaag acaagcccga tgaccacacc tgcctacacg cgcatcctgc
     1261 tgaagctctc cggcgaagcc ctcatgggcg acgatgcctt cggcatcaac cgggccgtgg
     1321 tcgagcgcat cgtcggccag gtgaaggaag tcgcggagct gggcgtgcag gtggggatcg
     1381 tgatcggcgg cggcaacatc ttccgcggcg tatcgcaggg tgcggcgggg atggaccgcg
     1441 ccaccgccga ctacatgggc atgctcgcca cggtcatcaa tgcgctggcg ctgcaggatg
     1501 cgatgaaccg ctcggggctc gtcgcgcgcg tgcagtcggc gctcaacatc gagccggtct
     1561 gcgagcccta catccgcggc aaggcgatcc gctacctcga ggaaggcaag gtcgtcatct
     1621 tcgcggcggg caccggcaac cccttcttca ccacggacac cgcagcggcg ctgcgcggcc
     1681 gcgaggtcga ggcgcagatc gttctcaagg cgaccaaggt ggacggcgtg tacacggccg
     1741 accccctgaa ggacccgaag gcgacgcgct acgacaggat cacctacgac gaggcgttcg
     1801 tgaagaacct gaaggtgatg gatgcgacgg cgctggcgct ctgccgcgac cagcgcctcc
     1861 cgatcaacgt cttctccatc ttcaaggaag gcgcgctgcg gcgcgtcgtg atgggggagc
     1921 cggaaggcac gctggtccac agctgaaacc cggaggacga atggctctga acatggccga
     1981 catcaagaag caggccgccg acaggatggc gaagtccatc gagaccctca ggctcgacct
     2041 cgccaaggtg cgcaccggcc gcgcccacgc cggcatcctc gaccacctgc gcgtggacta
     2101 ctacggcacg cccaccccga tcaaccaggt ggccaacgtg acgctggtcg acgcacgcac
     2161 catcggcgtc cagccctacg agaagaagat ggtccaggcg gtcgagcgcg ccatccgcga
     2221 ttccgacctg ggcgtgaatc ccgccacctc cggcgacatg atccgcgtgc cgatgccggc
     2281 cctcaccgag gagcgccgcc gcgagctcac caagatcgtg aagcacgagg gcgaggaggc
     2341 gaaggtcgcc gtgcgcaatg tccgccgcga cgccaacacg cacctgaagg agatgctcaa
     2401 gaagcacgac gttccggagg acgaggaaaa gcgcgtccag gacgaggtgc agaagctgac
     2461 cgacaaggcg gtgggcgaca tcgaccggat ggtcgccgaa aaggaaaagg aactgatggc
     2521 ggtctgatcc ccgcgaccgc cccccgagcc atggccgaaa tcgagagcag cacgctcgcc
     2581 attccccgaa ccggggacgt gccgcgccac gtcgccatca tcatggacgg gaacgggcgc
     2641 tgggcgaagt cgcgttacct gccgcgcgtg gcggggcacc gccgcggcgt cgaggccgtg
     2701 cgcgaggtgg tgaaggcctg catcgcgcgc ggggtggagt gcctcacgct cttcgcgttc
     2761 tcgagcgaga actggcgtcg ccccaaggag gaagtcacct tcctcatgca gctcttcctc
     2821 aaggccctcg agcaggaggt cgagaagctg cacggcaacg gcatccgctt caaggtgatc
     2881 ggcgaccttt ccgcattcga cccggtcatc gtggagcaca tccgccgcag cgaggcgctc
     2941 accgcgggca acgatcgcct caagctcacg gtcgcggcca actacggcgg gcgctgggac
     3001 atcctgcagg ccgccaaccg ctgccgcacc gagcatcccg gcgaggagat caccgaggaa
     3061 ttgctgtcgc gctatctctc catgcctttc gagcccgagc cggatctctt catccgcacc
     3121 ggcggcgagg aacgcatctc caacttcctc ctgtggcagc tcgcctacac cgagctgcac
     3181 ttcaccgagg tcctgtggcc cgacttcggc gccgaggcgc tcgatgaggc cttcgcctcg
     3241 taccgctcgc gcgagcggcg cttcggccgc acgagcgatc aggtcgccgc cacgcttccc
     3301 cagcccaagg tcggctagcc gccgtcccgg gcagcggcat gctcaagacg cgcgtcctca
     3361 cggcgatcgc cctgcttccc ctcgtcctgg gaatgctgtt cttctccggg ccggctgcgt
     3421 ggaccgcctt cgccacggcc atcgccctcg tctcctgctg ggaatggtcg cggctgtgcg
     3481 ggttcggccg cggctcgcga ggcctctacc tgctgctctg cgcggccatc gcgcttgccc
     3541 tcgccacggc cagcctgcgc ggcccggagc acgtgttcgc gaatgtcgcg caggcctcct
     3601 tcgtcgcagc ggcctgtttc tggatcttcg cggtgccggc ctggctcgcg cttcgcctgc
     3661 gccccgagcc gtgggcatcg ggggtcgcgg gctggttcgt gctctggccg atgtgggcgg
     3721 cactggtggt gctgcgcgag gcgagcccgt ggatcctgct ggcagccgcc gccctggtgt
     3781 gggtggccga catcgccgcc tatttcgccg gccggcgctt cgggcagcgc aagctcgcac
     3841 cggcaatcag cccgggcaag acctgggagg gtgtggccgg ggcgctcgtc ggcgtgctcg
     3901 cctacggcgt cgcactcgac ctctacgcgc atgcgcgtcc gggccccctg tcgccgctct
     3961 ttgggtcggc cggtggcgcg ctcgccgttc tcgcgatggt ggtgctggcc gcgttgtcgg
     4021 tgctgggcga cctgttcgaa tcgtggatga aacgcggcgc cgggcgcaag gactcgagcg
     4081 ggctgctgcc gggccacggc ggcatccttg accgcatcga tgcgctgacc cccacgctgc
     4141 ccgttgcggc actgctgctt tccttcgcat gaaacggctg accatcctcg gcagcaccgg
     4201 ctccatcggc gagagcacgc tggacgtcgt ggcgcgccac ccggaccgct tcgaggtggt
     4261 ggcgctcgcc gcccggcgcg gccacgagaa gctcgccgcg caatgcgagc ggtttgcgcc
     4321 gcgttacgcg gcgatggccg acgaggcggc cgccgagcgc ctgcgcgcgg cattgcgggc
     4381 caggggaatc gcgacggaag tgctggccgg cccggattca ctcgagcgcg tggccgcgct
     4441 tcccgagacg gacgcggtga tggccgcggt ggtgggcgcc gcgggcctgc cgggcacgct
     4501 cgccgcggcg cgcgccggca agcaggtgct gctggcgaac aaggaagccc tcgtcgtctc
     4561 cggctcgctt ttcctcgcgg cgctcgccga aggcggcggt acgctgctgc ccatcgacag
     4621 cgagcactcc gccgtcttcc agtcgctgcc gcgcggcttc acgtgcgggc gccagggcga
     4681 ggcggcccgt gccggcgtgc gtcgcatcct gctcacggcc tcgggcggcc ccttccgcac
     4741 cacgccgctg gagcgcctcg ggaatgtcac gcccgacgag gcctgcgcgc accccaactg
     4801 ggtgatgggc cgcaagatct cggtggactc cgcgacgatg atgaacaagg gcctcgaggt
     4861 gatcgaggcg ttctggctgt tcggcgcccc gagcgaggcg atcgaggtcg tcatccacgc
     4921 gcagagcgtg atccattcga tggtcgagta ccacgacggc tcggtcatcg cgcagctggg
     4981 cgccccggac atgcgcacgc cgatcgcctg cgcgctcgcc tggcccgagc gcatcgaggc
     5041 gggcgtggcc ccgctggatt tcgcatcgct ctcccggctc gagttcgggc gcgtcgacga
     5101 cgcgcgcttt ccctgcgtgc gactcgccta tgccgccctg cggcgcggcg ggacggcccc
     5161 ggccatcctc agcgccgcca acgaggtggc cgtcgagcac ttcctcgccg gcagccttgc
     5221 cttcaccgcc atcgcggcgg tgatcgagaa cgtgctgggg agcgtgaccg tcgagccgct
     5281 cgactcgctc gaggtcgcct ggcgagccga ccgcggggcg cgcgaggccg cgcggcgcga
     5341 ggtttcgcgc ctcgggcgcc gcgccggctg aaatcgggga caatcgagcc catgtccggc
     5401 gccttcgtca ccatcgcgtc cttcctcgtc acggtgggcc ttctcgtggt ggtccacgag
     5461 ctgggccact atgccgtggc gcgcctggcc ggcgtgaaga tcctgcgctt ctcgatcggc
     5521 ttcggacgtt ccctgtggat gcgccgcctc ggcaaggacc gcacggagtg ggtgatcgcc
     5581 gccttccccc tcgggggcta cgtgaagatg ctcgattcgc gcgaaggcga ggtggaaacc
     5641 gccgagcgcg cgcgtgcctt cgacctgcag ccggtgggca agcgcatcgc gatcgccctg
     5701 gccgggcccg ccgccaactt ccttgcggcc ttcctgctgt actgggcact gttcgtgatc
     5761 gggctgccgg gcgtgaagcc ggtggtcggc gacccgcccc gggcgagccc cgcggcggcg
     5821 gcggggctcg ccaacggcga caccatccgc gccatcgggg gcgaacctac cgccacctgg
     5881 acggacgtgc gctggctgct cctcaaggag gcggtgcgtg gcgaggacgt ggcgatcgag
     5941 gtggaaggcg agggcggcgc ccgcgcgacg cgctcgctcg ccctctcgtc gctcaccaag
     6001 gaagatctcg accgcgactt cctgatgaag ctgggcctga aggcgttcac gccgcacgtg
     6061 ccggccacgc tggggcgcat tgttcccggc ggtgcgggcg aacgcgcggg tctgcgcgag
     6121 ggcgaccatg tcgtcgccat cggcgggcag cccgtggcca cgtgggggca gttcacggcg
     6181 cgcgtgcgtg cctccgccgg gcgcacgctc tcgctcgagg tcgagcgctc gggccgccgg
     6241 ctggtcgttc ccgtggtgcc cgaagcgacg ggcgagggcg aggcgcgcat cgggctgctc
     6301 aaggtggagc cgggcgccga ggtgcgccgc gaatgggaac gcatgacgac cacggtgcgc
     6361 tacgacccgc tcgccgcggt cccgagggcg ctcgcccgcg tgtgggacct ctcggccttc
     6421 agcctgagga tgctcggcaa gatgctgctg ggcgaggtct cgtggaagaa cctttccggc
     6481 ccgatcacca tcgccgacta tgcggggcag tcggcgcaga tgggctgggt gtccttcgcc
     6541 gggttcctcg cgctggtgag cgtgagcctg ggggtgctca acctcctgcc catcccgctc
     6601 ctcgatgggg ggcacctggt gtactattcg gccgagatat tgaagggttc gccggtgtcc
     6661 gagcgtgcga tggaaatcgg ccagcgcgtg gggctcgcca tcctcgccgg gctcaccttc
     6721 ttcgccttct acaacgacat caaccggctc ctcacgggct gacgccttga aaaatccccg
     6781 cttccgctcc ctgcgcctcg ccatcgcctg cgcgcttgcc gcctcgcttc ccgctctcgc
     6841 catccagccc ttcgtggtga aggacatccg cgtcgagggc gtgcagcgca ccgaggccgg
     6901 caccgtcttc tcctacctgc ccgtcaaggt gggcgagcag atcgacgacc tgaaggcggc
     6961 gaaggccgtg aaggccctct acgcgaccgg gttctattcc gatgttcgcc tggaggccga
     7021 cggcgacgtc ctcgtcgtct tcgtccagga gcgcccggcc atcgcgggga tcgagatcga
     7081 gggcgccaag gaattcacca aggacaacct caaggacggg ctgaagcagg cgggcatcgc
     7141 ggaatcgaag atctacgaca agtcgctcct cgaccgcgcg gagaaggagc tcaagcgcca
     7201 gtacacctcg cggggcttct attcctcgac ggtgaggatc acggtgacgc cgctggagag
     7261 gaaccgcgtt tcgctgcgct tcgacatcga ggaaggcgag atcacgcgca tcgccgacat
     7321 caacatcatc ggcgcgaagg atttcaccga gcggcagctc ctgcgcgaga tccagctcac
     7381 cacccccggc tggctcacct ggatcaccaa ggacgaccag tattccaagc agaagctcac
     7441 cgccgacctg gagacgcttc gctccttcta cctgaaccgc ggctacctcg agttcgccat
     7501 cgactcgacg caggtctcga tcacgccgga ccgcgagaag atctacatca ccatcgcgat
     7561 caccgagggc ccggtgtacc ggctgggcga catcaagttc agcggcgacc tcatcgtggc
     7621 cgagcgcgag ctgcgcgagc tgctcagcct gcgcagcggc gacaccttct cgcgcgagaa
     7681 gatcatcgac agcgtgaagc gcatcaccga ccggctcggc aacgacggct attccttcgc
     7741 caacgtgaac ccggtgccgg acctggaccg ccagaagcgc gtggcgggct tcaccttctt
     7801 cgtcgatccc ggccggcgcg tctacgtgcg gcgcgtgaac atcgtgggca actcgcggac
     7861 ccaggacgcc gtgatccgcc gggagcttcg ccagctcgaa agctcgtggt actcgctgga
     7921 gaagatcgcg cgctccaagg aacgcctgca gcgcaccagc tacttctcgg aagtcaacat
     7981 cgagaccccc gccgtggccg gcaccgcgga ccaggtcgac gtcgtggtga cggtcacgga
     8041 aaagaacacc ggcagcctca atttcggcgt cggctattcg caggtggaca agctcacggt
     8101 gtcggcgtcg gtcgcccagg ccaacctctt cggatccggc aaccagctcg cgttccaggt
     8161 gaacaacggc tcgatcaacc gggtctactc gctttccttc atcgacccgt actggacgat
     8221 cgacggcatc gcggccggca tcgacatctt ccgcagggac gtcgacacct cctcgctggt
     8281 ggtgacctcc tacaagacga cctccagcgg cgtgggcgtg aacttctcgg tgccgatcac
     8341 ggaatacgac acggtgcgcg tcgggctcac cgcggaggcg acgaagctcc agctcgacac
     8401 ggcggccgcc ccggcgcgct acctcgattt cgtgaacacg tacggcgagc ggaccaacac
     8461 cctgcggacc aacgtcggct acgcgcgcga cacgcgcgac agcctcacct acccgacccg
     8521 cggctggctc accgagttcc tgctcgagat cggcattccg ccgggcgacc tcaagtacta
     8581 ccgcgcctcg gcccagcagc agttcctgtg gacgccggac cggctgagct ggctcacctt
     8641 cctggtgaac gcggagttcg gctatgccga cggctacggg ggcaagccgc tgcccttctt
     8701 caagaacttc tacgccggcg gcgtgggctc cgtgcgcggc ttcgaggccg cctccctggg
     8761 gccgcgcgac ctgaacgggg atgtgctcgg cggcaaccgc cggctgatcg gcaacctcga
     8821 gctgctcttc ccgatgcccg gcatcaagga caagagcgtg cgcctgtcca ccttcctgga
     8881 cgcgggcagc gtctggggct cggacgagaa gatcgtcggt gccgacatcc ggttgtcgac
     8941 gggagtggcg gtgagctggg actcgccggt ggggccgctc aagttctcgt tcagcgcgcc
     9001 gatcaagaag aaggccgacg acaagctcga gcgcttccag ttccagttgg gcaggatctt
     9061 ctagaaaggc ggaacatcgc gtatggactg gcggacagga ttggcggcat cgctcggcgc
     9121 gatcgcgttg ggcggcatgg ccgcggaggt gaagatcggc ttcgtcaaca cggagcgcgt
     9181 gttccgggag gccgcgccgg ccaagcgcgc gcagcagaag ctcgagcgcg agttctcgac
     9241 gcgcaatgcg gagctcgcca agctggagaa gcacgggcgc gacctgcagg tcgagctcga
     9301 gaaggacagc gtgacgctcc ccgaggcggc gcgccgggaa aaggaaaggc agctcgccga
     9361 cgtgagccgc aacttccagc gcctgcagcg cgagatccgc gaggacctca acctgcgccg
     9421 caacgaggag ctcgcgcagg tccaggagcg cgccacccgc gtcatcaacc agatcgccga
     9481 ggccgagaag ttcgacctgg tcgtgcagga ggccgtgttc gcgagctccc gcatcgacat
     9541 caccgacaag gtcatcaagg cgctcgcgga caagtagccg cgcgaggccc ccttgccccc
     9601 ccagagcccc accgccaggg atcctgcctt caccctgcag gagatcgtcc gccgcctcgg
     9661 cggcgaagtc gcgggggatg cctcggtcac ggtcaacggc gtggcggcaa tcgacgaggc
     9721 cggccccggc gagatcacct tcctcgccaa tccgcgctac cgctccaggc tcgccgccac
     9781 gcgcgccggc gcggtgatcc ttggtccgcg cgaccgcgac gcgacggcca tcgcgcgcat
     9841 cgtcgcggac aacccttatg cctactacgc gcgcacggtt gcgctgttca atccgccgcg
     9901 ggtggtggtc cccggcacgc acccgacggc ctgcgtcgat ccgggcgcgc gtgtcgcggc
     9961 ctcggccgag atcgggccct tcgcggtgat cgccgccggg gcgcgcgtcg gcgagcgcgc
    10021 atgcatcggc gcgggatcgg tggtgggcga gggcgcctcg atcggcgacg acacgatcct
    10081 ctacccgcgg gtcacggtct atcacgcctg cgtggtgggc gcgcgctgca tcctgcattc
    10141 gggagcggtc atcggcgccg acggtttcgg catggcgccg gacgcgggcc gctgggtgaa
    10201 gattccgcaa gtcgggcgcg cggtgctggg cgacgacgtc gaggtcgggg ccaacaccac
    10261 gatcgaccgc ggcgcgctcg gcgacacggt ggtcgaggac ggggtgaaga tcgacaacca
    10321 ggtgcaggtg gcgcacaact gccgcatcgg cgcgcacacc gtcatcgccg gctgcgtggg
    10381 catttcgggc agcgtcgtca tcggacggca ctgcctgatc gggggcggcg ccggcatcgt
    10441 cggccacatc accctgtgcg acggcgtgac cgtgtcggga atgaccttca tcaccaagtc
    10501 catcacgcgg ccgggggtgt actcgtcggg gatgccgatg atgccgcatg ccgactggct
    10561 gaggaacgcg gcgcagcttc gccgcctcga cgagaccgcg cgcctcgccc gcgggaaaag
    10621 cgcaccggga gaagaacgtg aagacgatga cgattgacga aatcaaggcg ttcctgccgc
    10681 accgctaccc gttcctgctc atcgatcgcg tggtcgattt cgagaaggac ctgcgcatcg
    10741 tggcggtgaa gaacgtcacg gccaacgagc ccttcttcca ggggcacttc ccgcatttcg
    10801 cggtgatgcc cggcgtgctc gtcgtcgagg ccatggcgca ggccgcggcc ctcctgtcgc
    10861 tgcggtccct gggccggaag aacgacgcca agagcgtcta ctacttcgtc ggcatcgacc
    10921 gcgcgcgctt caagcgcccg gtcgtccccg gcgaccagct gatgatcgag gtcgagcagc
    10981 tgcgcctggc gcgcggcatg gggaagttcg cggccgtcgc gcgcgtcgac gggtccgttg
    11041 ccgcggaggc ggagctcctg tgcaccctgc ggcccatcgg gtgaggcgcg gcccgcgatg
    11101 accgcgatcc accccaccgc catcgtcgac ccgcgggcgc ggctggccgc gggcgtgcgc
    11161 gtcggcccct acaccgtgat cgatggcgac gtcgaggcgg gcgagggcac caccttcggc
    11221 gcgcactgcg tggtgacggg ccacacgacc ctcggccgcg acaaccgcat cttccacttc
    11281 tgctcgatcg gggaagcctg ccaggacaag aagtacggcg gcgagcccac gcgcctggtg
    11341 atcggcgacc gcaacaccat ccgcgagtac tgctcgctca atcgcggcac cgtgcagggc
    11401 gggggcgtca ccaccgtcgg ccacgacaac tggatcatgg cctacagcca catcgcgcac
    11461 gactgccgcg tgggcgacca caccgtcttc gccaaccacg ccacgctggc cggccacgtc
    11521 gacatcggcg accacacggt gctcggcggt ttcaccggcg tgcaccagtt cgtgaaggtc
    11581 ggcgcgcacg tgatggccgg ggtctcgtcg gtggtgctgc aggacgttcc cccgtacgtc
    11641 accgttgccg gccacccttg ctcgccgcac ggcatcaaca gcgaggggct caggcggcgc
    11701 ggcttcactc ccgaggcgct cgcggcgctc aagcgcgcct acaagacgct gtacaagtcc
    11761 gggctcgccc tcgccgaggc gcgcgccgag ctcgagcggc agtcgctggc ggcgcccgaa
    11821 gtgaaggcga tcgtcgactt cctcgccacg tcgacgcggg gcatcgtgcg ctgatggggt
    11881 ggccggtgaa cgagccgtgg ggatgaaatg actcgtcgcg tggccatcgt cgccggcgag
    11941 gcctcgggcg acatgctcgg ggcgtcgctg gtgcgcgcgg tgcgcagccg ctttcccgac
    12001 acgcagttct acggcatcgc cggcccgcgg atgatggcgg cgggggccag cacgctggtg
    12061 ccgatggaga agctctcggt gcgcggctat gtcgaggcga tgcgcagcct gccggagctg
    12121 ctgcgcatcc gctcgcggct cgctgcccgg ctcgtgtccg accggcccga cctcttcatc
    12181 ggcgtggacg cgccggactt caacctgggg ctggaggcgc ggctcaagcg cgcgggcata
    12241 ccgaccgtgc attacgtgag cccgtccatc tgggcctggc gcgcggggcg gctgccttcc
    12301 atcgggcgcg cggtggaccg ggtgctcacc atctttccct tcgaggccgc cctctacgag
    12361 aaggccggca tcgccgccac ctacgtgggt catccgctcg ccgacgagat cccgttcgtg
    12421 ccggaccgcg ccgatgcgcg cgcgcagctt cgcctggatg aagcgacgac ggcggtggcg
    12481 ctccttccgg gcagccgccg cggcgagctc gaggcgcacg ccgacctgtt catcgacacg
    12541 gcgcgccggc tgtccgcgcg ccgccccggg gcgcgcttct tcgtccccct cgccacgcgc
    12601 gagacgcgcg acttcttcga gtcgcggctg tacgcccggg aggcgcagga actcgacctc
    12661 accatcctgt tcggccacgc ccggcttgcc ctgcaggcgg ccgactgcgc gctggtcgcg
    12721 agcggcaccg ccacgctcga gggagcgctg gcgcgctgcc cgatggtgat cacctacaag
    12781 gtccccgcgc tcacctaccg gctcatgatg cgcaaggcgc tgcttcccta cgtgggcctg
    12841 cccaacatcc tcgcgggcga gttcatcgtg ccggaactgc tgcaggaaca cgccacgccg
    12901 gagaacctcg agcaggcgct ggccaactgg ctcgaccaca aggtcgcgcg cgcgcggctt
    12961 gtcgagcgct acgaggcgat ccacgcgcag ctgcgctgcg gcaacgacga gcgcgtggcg
    13021 caggcgctcg cgccgtggct gtcgaacgcg caggaaacgg ggacgctgtc tcttgaacaa
    13081 ctcgccttca aagggtagcg tcccccggtc gtgcggggtc gacgaggccg ggcgcggccc
    13141 gctcgccggg gcggtgttcg cggccgcggt catcctcgat ccttcacggc ccgtgccggg
    13201 gctcgccgat tcgaagctgc tgagtgccgc acggcgcgag gcgcttgcgc gggtcatccg
    13261 gcgcgacgcg ctcgcctggg ccatcgcctc ggccaccgtc gaggagatcg acgccatcaa
    13321 catcctgcag gcgagccttg cggccatggc gcgcgccgtc gcggcgctgg gcgtcgaccc
    13381 cgacgaggtc gtcgtcgacg gattgcacgt gccgcggatg cgctggccgt cgcgggcgat
    13441 cgtgaagggc gaccgcctgg tccctgcgat ctcggcggcg tcgatcctcg cgaagaccgc
    13501 gcgcgacgcg gagatggtga ttctagacgc gcgcttcccc ggctacggct tcgcgcgcca
    13561 caagggctac cccaccgccg accacctcgc cgcgctgcgc tcgctcgggc cctgcgcgat
    13621 ccaccggcgc agcttcgagc cggtgcgcgc ggtcatcgcc ctgtgcgacc cggcgttctc
    13681 acgatgagga acatcgcctc gcgcggcgtt ccgttgcggc gatgaagacc atcgcctcgc
    13741 gcgacaacgc cgcctacaag tcactggcca ggctgtgcac gtcggccgcc gagcgcagga
    13801 agcgcggcct ttccgtcatc gagggggcgc atctcgtcga ggcttgtctc gacgcgggac
    13861 acccggtcgc gcaactcttc gtgagcgcgg cggccaccgg ttccgcgcag gcgacggcgc
    13921 tgcgcgcgcg cgcgaaggcc gcagaaatgg tggcgctctc cgatgccctg ttcgacgcgg
    13981 tgagcggcgt ggagtccccg gccggaatcc ttgccgttgt tcccacgccc gaggggcgcg
    14041 cggtgccggc ggatgcgcgc ttctgcctcg ccctcgagga cctgcaggat cccggcaacg
    14101 tgggcacgct cctgcgcagc gccgcggcgg cgggcgtcga gcacgtgctg ctctctccca
    14161 cctgcgcctt cgcctggtcg cccaaggtgc tgcgcgcggg gcagggggcg catttcgcgc
    14221 tgaacatcgt ggaggggacg gatctcgccg cgttcgtcgc gtcgttcgcc ggcacgacca
    14281 tcgcgctggc gggaaaggcg aagcgctcgc tctacgaaac ggacctgcgc ggccccgtgg
    14341 cattcctcgt cggcaacgaa ggcgcgggac tgtcggcgga actggagcgc tccgtcacca
    14401 tacgcgcgcg cattccgatg cccggctgcg ccgaatccct aaatgccggc gtcgccggct
    14461 ccatcgcgct cttcgaagct gttcggcagc ggggagtgac gctacccaaa ggcgggggac
    14521 acggatgaac acggatgaac acggataatt cttgaccggg aaccgccgtc gacctgttca
    14581 ccgtcgactc gaatcggcac ggggcgtcca gccctcgatt catatccgtg ttcat
//
LOCUS       DASUEV010000202         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_737932, whole genome shotgun sequence.
ACCESSION   DASUEV010000202 DASUEV010000000
VERSION     DASUEV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737932"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFV55_06570"
     CDS             <1..783
                     /locus_tag="VFV55_06570"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX4944000.1"
                     /translation="PTDLNAMPAADLPLNDKVVLVTGAARRIGAAIAQAFHAAGAKVA
                     VHYHRSAAEAGRLVAAMNAERPGSAAAFGADLAVLAALEVLPAEVIGTLGALDVLVNN
                     ASTFYPTPVGKITPTQFEDLVGTNLRAPLFLSQAAAPELRRRKGLILNIADIHGLRPL
                     GQHSVYCAAKAALVMLTQSLARELGPEIRVNAIAPGPVMWPENGVDEELRQRIVARTA
                     LKRGGSPNDIAKTAIFFATDAPFVTGQVLAVDGGRLIGGEDR"
     gene            complement(792..1286)
                     /gene="folK"
                     /locus_tag="VFV55_06575"
     CDS             complement(792..1286)
                     /gene="folK"
                     /locus_tag="VFV55_06575"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX4944001.1"
                     /translation="MPEVFVAAGSNIRPRTHLRRAVAALAAAYPGLRVSRAWSNAAVG
                     FEGDDFINLVVGFETDDALAKVLERLKSVEEASGRERGAKKWAPRTLDLDLLLYGDLV
                     GQFPGATLPRADLAECAYVLGPLAELAPQLRHPVLGATLGRLWKDFDRSSHTLREVSL
                     DGED"
     gene            complement(1279..1635)
                     /gene="folB"
                     /locus_tag="VFV55_06580"
     CDS             complement(1279..1635)
                     /gene="folB"
                     /locus_tag="VFV55_06580"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX4944002.1"
                     /translation="MDKIFLTSLSTEAVIGIFDWEREVRQRIEIDLEMSFDLSAAAAS
                     DSIDDTLNYKSVAKRVLAFVEGSRFQLVETLAERVAALVLAEFPVAKVRVTVHKPGAI
                     RHSRDVGVIIERSRDA"
     gene            complement(1644..2663)
                     /gene="tsaD"
                     /locus_tag="VFV55_06585"
     CDS             complement(1644..2663)
                     /gene="tsaD"
                     /locus_tag="VFV55_06585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX4944003.1"
                     /translation="MRVLGIETSCDETAVAVYDGERGLIANRVWSQIELHRPHGGVVP
                     ELASREHVERLLPMIEECLADAGTTPQAIDGIAYTAGPGLIGALLVGATVARGLGYAW
                     DVPVVGVHHLEGHLLAPRLEPGAPDFPFVALLVSGGHTMLVEVAGIGQYRILGETLDD
                     AAGEAFDKTAKLLGLGYPGGPELAALAESGRDGVFEFPRPMLDRPGLDFSFSGLKTAV
                     VVALRGRKLDAQGRADAARGFESAIVETLVAKALRAMRLTGVKALVVAGGVGANRRLR
                     AELTEAVAAADGRVHYPRPEFCTDNAAMIALAGHERLAAGEREALPVRARARWPLDEL
                     AAPRH"
     gene            2760..2972
                     /gene="rpsU"
                     /locus_tag="VFV55_06590"
     CDS             2760..2972
                     /gene="rpsU"
                     /locus_tag="VFV55_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX4944004.1"
                     /translation="MPSVRIRENEYFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAIKRHLKRATRDGGRSATR"
     gene            2980..3426
                     /locus_tag="VFV55_06595"
     CDS             2980..3426
                     /locus_tag="VFV55_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX4944005.1"
                     /translation="MTLKARITEDMKTAMRSGEKDRLSCIRMLQAGIQQREVDERITL
                     DDAQVLAVIDKMIKQRRESVAQFQAGKRADLVAKESAEIAWLTQYLPAQLGDAELEAL
                     IREAIAATGAASMKEMGRVMGWLKPKVQGRTDIGALSARIKAALGG"
     gene            3431..4252
                     /locus_tag="VFV55_06600"
     CDS             3431..4252
                     /locus_tag="VFV55_06600"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX4944006.1"
                     /translation="MSPSKVPEGEMRGFIVPIGGAENKENDPRILERFVRVSGGSDAD
                     IVVIPTASRAHETGPRYEQLFRDLGAAHVAVMDFDTRRDCQEKGRLERLAKATGIFFT
                     GGNQLRLTTLLGGTPVAKLIRSQNARGVNIGGTSAGASILSEHMIAFGDDGSSAIAGS
                     VRLAPGLGLTNRFIIDQHFRQRDRLGRLITALAYNPFAVGIGLDEDTAAFIGPDETLE
                     VEGSGGVTVVDASKVTFSSVDSAAEGQPICILGLVVHILVAGSTFNLHTRVASTG"
BASE COUNT          754 a         1557 c         1447 g          675 t
ORIGIN      
        1 cccaccgacc tgaacgcgat gcccgcagcc gacctgccat tgaacgacaa ggttgtactg
       61 gtcaccggcg cggcgcgccg gatcggtgcg gcaatcgcgc aggcgtttca tgccgcgggc
      121 gcgaaggtcg ccgtgcatta ccaccgctcc gccgccgagg ccggacggct ggtggccgcg
      181 atgaatgcgg aacggccggg gtccgcagcg gcattcggcg ccgatctcgc cgtcctggcg
      241 gcgctcgagg tgcttcccgc ggaggtgatc ggcactcttg gcgcgctcga cgtgctcgtc
      301 aacaacgcgt ccacctttta tccgacgcca gtcgggaaga ttacgccgac ccaattcgag
      361 gaccttgtgg gcacgaacct gcgcgcgccc ctgttcctgt cgcaggctgc cgcgcccgaa
      421 ttgcgccggc ggaagggtct catcctgaat atcgccgaca tccatgggct gcggccgctc
      481 ggccagcatt cggtctattg cgcggccaag gccgcactcg tgatgctgac gcagtcgctc
      541 gcacgcgaac tcggccccga aatccgcgtc aatgcgatcg cgcccggacc tgtgatgtgg
      601 cccgagaacg gcgtggatga ggaactgcgc cagcgcatcg tggcacgcac tgcgttgaaa
      661 cgcggcggct cgccgaatga tatcgcgaaa acagcgatct tcttcgcgac cgacgcgcca
      721 tttgtgaccg gccaggtgct ggctgtcgat ggcgggcgcc tcatcggcgg cgaggaccgc
      781 tgaccgccgc atcaatcctc accgtcgagc gagacttccc gcagcgtgtg gctgctgcgg
      841 tcgaaatcct tccacagtct gccaagagtc gcgccgagca ccgggtgccg cagctgcggc
      901 gcaagctcgg cgagcggacc gagcacatag gcgcactcgg ccaggtccgc acgcggcagc
      961 gtcgcgccgg ggaattgccc gaccaggtcg ccgtacaaca gcaggtcgag gtccagcgtg
     1021 cgcggcgccc atttcttcgc gccacgctcg cgaccactgg cctcctcgac tgatttcagg
     1081 cgctccagca ctttcgcgag cgcgtcatcg gtctcgaagc caaccaccag gttgatgaaa
     1141 tcgtcgccct cgaacccgac ggcggcattc gaccaggcac gcgagacgcg caagcccgga
     1201 tacgcggcag caagcgccgc gacggccctg cgcaggtgtg tgcgcggccg gatattgctg
     1261 cccgccgcga cgaagacctc aggcatcccg gctgcgctcg atgatcacgc cgacgtccct
     1321 cgaatgccgg attgcgcccg gcttgtgtac cgtcacgcgc acctttgcga ccgggaactc
     1381 cgcgagcacc agggccgcga cgcgctcggc cagggtttcg acaagctgga agcggctccc
     1441 ttcgacaaac gcgagcacgc gcttcgcgac cgacttgtaa ttgagcgtgt cgtcgatgga
     1501 gtcactcgcg gccgcggcgg acaggtcgaa cgacatctcg aggtcgatct cgatccgctg
     1561 ccggacctcg cgttcccagt cgaagatccc gatcacggcc tcggtcgaaa gcgacgtcag
     1621 gaagatcttg tccacaatcc ctctcagtgc cgcggcgcgg ccagttcgtc cagcggccag
     1681 cgcgcacggg cgcgcacggg caatgcttcg cgctcgcctg ccgccaaccg ctcatggccg
     1741 gccagcgcaa tcatcgccgc attgtccgtg cagaattccg ggcgcgggta atggacgcgg
     1801 ccgtccgccg cggcaaccgc ctccgtcaac tccgcgcgca ggcgccggtt cgcaccgacg
     1861 ccgcccgcga cgaccagcgc cttgacgcca gtgaggcgca tcgcgcgaag cgctttggcg
     1921 accagtgtct cgacgattgc cgactcgaat ccgcgcgcgg cgtccgcgcg cccctgcgca
     1981 tcgagcttgc gcccgcgaag cgcgacgacc accgccgtct tcaggccgct gaagctgaag
     2041 tcgaggccgg gccggtcgag catcggccgc gggaattcga acacgccgtc acgccccgat
     2101 tccgcgagcg ccgcgagttc cggcccgccc gggtagccga gcccgagcag cttggccgtc
     2161 ttgtcgaagg cctcgccagc ggcgtcgtcc agggtttcgc cgaggatccg gtattgcccg
     2221 ataccggcga cctcgaccag catcgtgtgg ccgccggaga ccaggagcgc gacgaacggg
     2281 aagtccgggg ctccgggctc aaggcgcggg gccagcagat gaccttcgag gtgatgcacg
     2341 ccgaccacgg gcacgtccca ggcatagccg aggccgcgcg ccaccgtcgc cccgaccagc
     2401 agcgcaccga tgaggccggg tccggccgta taggcaatgc catcaatggc ttgcggagtg
     2461 gttccggcgt ctgcgaggca ttcctcgatc atcggcagca accgttccac gtgctcccgc
     2521 gaggcgagct cgggcacgac gccgccgtgg ggacggtgca gctcgatctg gctccacacg
     2581 cgattggcga taaggccccg ttcgccgtcg tacacggcga ccgcggtctc gtcgcaggag
     2641 gtttcgattc cgagcactct catggagcta gtcttttgat tttccggggg cagatcacta
     2701 caattccggg ccctttcccc ggggcacttc cggggagtcg cctgccagac gaggtttgaa
     2761 tgccgagcgt ccgtatccgc gagaacgagt atttcgacgc cgcgttgcgc cgtttcaagc
     2821 gcgcctgcga aaaggccggc gtgctgactg aactgcgtcg ccgggaattc tacgagaagc
     2881 ccacccagga acggaaacgc aagaaggccg cggcgatcaa gcggcacctc aagcgcgcca
     2941 cgcgcgatgg tggccgcagc gcgacacgct gatcccggga tgaccctcaa ggcccgcatc
     3001 accgaagaca tgaagactgc gatgcgctcg ggcgagaagg atcgcctatc gtgcatccgc
     3061 atgctgcagg cgggaatcca acagcgggaa gtggacgagc ggatcacgct cgacgacgca
     3121 caggtgctgg ccgtcatcga caagatgatc aagcagcgcc gcgaatccgt cgcccagttc
     3181 caggccggca agcgcgccga cctggtcgcg aaggaatccg ccgagatcgc ctggctgacg
     3241 caatacctcc ccgctcagct cggcgacgcc gaactcgagg cgctgatccg cgaagccatc
     3301 gccgccaccg gcgccgcatc catgaaggaa atgggcaggg tcatgggctg gctgaagccg
     3361 aaggtccagg gccgcaccga catcggcgcg ttgtcggcgc gcatcaaggc cgcgctcggc
     3421 ggttgatggc atgagcccca gcaaggttcc cgagggcgag atgcgcggct tcatcgtgcc
     3481 gatcggcgga gccgagaaca aggaaaacga cccgcgcatc ctcgagcgct tcgtgcgcgt
     3541 ttcgggcggc agtgacgccg acatcgtcgt catcccgacc gcgagccgcg cgcatgagac
     3601 tggcccgcgt tacgaacagc tgttccggga tctcggcgcg gcgcacgtcg ccgtgatgga
     3661 tttcgacacg cggcgcgact gccaggaaaa gggccggctc gaacgcctgg ccaaggcgac
     3721 cggcatcttc ttcaccggcg ggaatcagct ccgcctcacg acgctgctcg gcggcacgcc
     3781 ggtcgccaag ctgatccgct cgcagaacgc ccgcggcgtg aacatcggcg gcacgagcgc
     3841 cggcgccagc attctgtcgg aacacatgat cgcattcggc gacgacggat cgtcggcgat
     3901 cgcaggcagc gtgcgtcttg cgccgggcct cgggctgacc aaccgcttca tcatcgacca
     3961 gcatttccgg cagcgcgacc gcctcggccg gctgatcacg gcgctcgcct acaatccctt
     4021 cgcggtcggc atcggcctcg acgaggacac cgccgcattc atcgggcccg acgaaacgct
     4081 cgaggtcgag ggcagcggcg gcgtcaccgt cgtggacgca tcgaaagtca cgttctcgtc
     4141 cgtggacagc gccgccgagg gccagcccat ctgtattctc ggactcgtcg tgcacatcct
     4201 ggtcgcgggc tcgactttca acctgcacac gcgggtcgcc tcgacgggct gattcggtcc
     4261 atgcgcatac tcgaccgatc catctacgtc gggccatcgc atttcgcgcg cttcccggtc
     4321 atacgcctcg agctcgacct cggggaactc gaggcctggc ccacgggccg gctcggcaag
     4381 cccttcgtgg acagcctcgt cgaggcgttg cccggcctcg ccgagcacgg ctg
//
LOCUS       DASUEV010000203         8690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_387335, whole genome shotgun sequence.
ACCESSION   DASUEV010000203 DASUEV010000000
VERSION     DASUEV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387335"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFV55_06605"
     CDS             complement(<1..846)
                     /locus_tag="VFV55_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEX4944007.1"
                     /translation="MDRREFLNVLAIAAAAGLPIASREALAAADGAKLYDIPRFGNVS
                     LLHMTDCHAQLLPIYFREPDVNLGIAGAAGRAPHLVGASLLKAFGVQANSAQAHAFTY
                     LDFAQAARTYGKVGGFAHLATLVKQLKASRPGALLLDGGDTWQGSATALWTRGQDMVD
                     AGLALGVDVMTAHWEFTLGAERVKQVVENDFKGRIDFVAQNVATADFNDPVFAPFVLK
                     QINGVPVAIVGQAFPYTPIANPRHMVPDWTFGIREPELQKAVDEARAKGARVVVLLSH
                     NGMDVD"
     gene            complement(865..1365)
                     /locus_tag="VFV55_06610"
     CDS             complement(865..1365)
                     /locus_tag="VFV55_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX4944008.1"
                     /translation="MPNALASAALTLLLACGSAIAAEPATFTVKALTPETALKAAQAA
                     LAKCRADGYQVSVAIVDRSGLVQVVVRDRYAGAHSTDTATNKAWTAVSFRTNTADLLK
                     ITQPGQPSSGIRNLPRVVAVGGGVLIEAGGSILAGIGVSGAPGGDADEACAKAGIKAI
                     AEALEF"
     gene            complement(1427..2044)
                     /gene="soxX"
                     /locus_tag="VFV55_06615"
     CDS             complement(1427..2044)
                     /gene="soxX"
                     /locus_tag="VFV55_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX4944009.1"
                     /translation="MKQRTKIISLAAVAAALAGCATMAPDYDKESMALMKRDFKTRGQ
                     ATLSRLDQDELQATCSALSGAHPPKDVAQRLEAGQQALIKYPSNGILLGDWTKAEAIA
                     QMGQGGQWSDDPKKPSGGNCYACHQLTRKEISYGNIGPSLYNYGKTRGNSPEVQKYTY
                     SKIYNSQAFAACSNMPRFGHNGVLTEAQIKDLVAFLLDPKSPVNQ"
     gene            complement(2058..2879)
                     /gene="soxA"
                     /locus_tag="VFV55_06620"
     CDS             complement(2058..2879)
                     /gene="soxA"
                     /locus_tag="VFV55_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX4944010.1"
                     /translation="MNRTQELAARAACAAFVLSAAGAFAQERSAVEEIERYRQSLADG
                     NPAELWEARGEDLWKQKRGPKGASLERCDIGLGPGVVKGAYTVLPKYFPDTDKVQDLE
                     SRLVTCMVSLQGFTVEQATKQHFGAGNDKKSDMEAIVAYITGSSRGMKMNVAATHPKE
                     REAYEIGKRMFFHRGGPYDFACASCHGEEGKRIRLQDLPNLLNPKDAQRAYGAWPGYR
                     VSQGEVRTMQHRLFDCYRQQRFPEPVYASDAVSALTLFLAKNANGGVFNAPALKR"
     gene            complement(2936..3247)
                     /gene="soxZ"
                     /locus_tag="VFV55_06625"
     CDS             complement(2936..3247)
                     /gene="soxZ"
                     /locus_tag="VFV55_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX4944011.1"
                     /translation="MADPMKIRAAMSGDKVEVKVLMSHEMETGQRKDAKGAVIPAHFI
                     QSVTATHNGKVVLAAEWGPAVSKNPFLSFKFSGGKPGEKVSITWTDNKGDKRTDEATI
                     S"
     gene            complement(3261..3731)
                     /gene="soxY"
                     /locus_tag="VFV55_06630"
     CDS             complement(3261..3731)
                     /gene="soxY"
                     /locus_tag="VFV55_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX4944012.1"
                     /translation="MNDDRRKVLKGTGSMAVMGLAASAGLFRPGSAWAQAWNKAAFET
                     KNMADTVKAMGGAAATESKEIAIASPDIAENGAVVPFTISSRLPKTESIALLVEKNPS
                     ALAASFTIPAGTEAGITTRVKMGQTSNVIALVKADGKFYYTAKEVKVTLGGCGG"
     gene            4048..4383
                     /locus_tag="VFV55_06635"
     CDS             4048..4383
                     /locus_tag="VFV55_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4944013.1"
                     /translation="MAPNRAKAVRHDIFDLTGRGEDIRQASEALKAMAHPLRLKILCL
                     VGQGELMVQEIVEAVGTSQSNISQHLSVMRERGLLSSRKEANKVFYRIEDPRILKMIA
                     MMREVFCTL"
     gene            complement(4412..4885)
                     /gene="moaE"
                     /locus_tag="VFV55_06640"
     CDS             complement(4412..4885)
                     /gene="moaE"
                     /locus_tag="VFV55_06640"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEX4944014.1"
                     /translation="MAHVRVQEAPFDAGREIAALTEGRRDVGAVASLVGLVRDRNEGA
                     EVSRMTLEHYPGMTERALQDICAEAARRWNVIDARVVHRVGPLEPGEAIVLVAVSSAH
                     RGEAFAACEFIMDFLKTRAPFWKKESVPSGERWVEARASDDEAAARWTGDGEGHP"
     gene            complement(4889..5143)
                     /gene="moaD"
                     /locus_tag="VFV55_06645"
     CDS             complement(4889..5143)
                     /gene="moaD"
                     /locus_tag="VFV55_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX4944015.1"
                     /translation="MITLLYFAGLRERLDCAREQVALPAGTPTVGTVVDRLRSRDGRW
                     SEAFAPGRAWRVAVNQRMADLATPLKPGDEVAFFPPVTGG"
     gene            complement(5174..5701)
                     /gene="mobB"
                     /locus_tag="VFV55_06650"
     CDS             complement(5174..5701)
                     /gene="mobB"
                     /locus_tag="VFV55_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX4944016.1"
                     /translation="MKILGIAGYSGSGKTTLIEKLVPILVKEGLRVSLVKHAHHRFEV
                     DHPGKDSYRHRHAGCNEVLVSSSRRWALMRDLRGDAEPTLDELLRRFSPCDLVLVEGY
                     KHDPIPRIEVHRAGGDRPLLFPRDPHVIAVATDEPLETRLPQFGLDDIPAVARFIREF
                     VAGREGRARLSVVPD"
     gene            complement(5756..7246)
                     /locus_tag="VFV55_06655"
     CDS             complement(5756..7246)
                     /locus_tag="VFV55_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4944017.1"
                     /translation="MDSLDHLAQGFIVAARPVNLLVAFLGSFIGTIVGLLPGLGPING
                     VAILLPIAFAMKLPPETALILLASVYIGCEYGGRISSILLNIPGDAGALMTALDGYPM
                     AKKGLAGVALSISAWSSFVGSIIATIGLALFAPILARWALAFGPAEYFVLMVFAICCL
                     AGMVGDRPVKTLLSALIGLFLATVGIDAGSGAYRFTFDDVHLSDGIQFIVVVIGLFSV
                     SEMLVMLEQSHAGQVVIRNTGRAMFNLKEMGETWWGTVRSSVIGFVIGVLPGAGATIA
                     SAITYMVEKNLTDREGTFGTGDIRGVAAPEAANNSAAGGAFVPMLTLGVPGSGTTAVM
                     VGALTLYNINPGPLLFEQQPELVWGLIASMFIGNVMLLVMNIPMVGLFARLLYVPNWI
                     LVPGIAAVSFVGVYAVHATTFDLVLMVALGIFGWALRKLGFPMAPLILGFVLGDMMEQ
                     NLRRALSISDGHVGILFHSPVSLVLWVLAAAVLLIPMLLRRLKRQA"
     gene            complement(7246..7683)
                     /locus_tag="VFV55_06660"
     CDS             complement(7246..7683)
                     /locus_tag="VFV55_06660"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4944018.1"
                     /translation="MSDRVFAFLWLVVCAAIAWLARMIEAPFSYEPIGPRAFPLLLAA
                     LMALSAAWLLVRPDREPDWPRGALRAKSLLLIVAFVAYAMFFEWLGFVVATALATVVI
                     GRLFDGSWKGCVLAGIALGGGLWYFFDRLLDVTLPLGRLWALA"
     gene            complement(7739..>8690)
                     /locus_tag="VFV55_06665"
     CDS             complement(7739..>8690)
                     /locus_tag="VFV55_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX4944019.1"
                     /translation="VSALAAVTSLAAFATIDKPECIVGAKPGGGFDLTCKLAQQSLLD
                     LKLLKDPMRATYMPGGIGAVAMNTVVAQRAADANVIVAFSGGSLLNIAQGKFGKWTEN
                     DVRWLAAVGTDYGSISVGKNAPWKNLAEVAAAIKADPSKVPMGGGGTVGSQDWTKAAL
                     LAKKLGVDPKAMRWVSFEGNGEATTALMGGHIQVMFGDVSADEAQAEAGNIRMIAVLA
                     DKRIDGKLAGIATAKEQGYDLQWPIIRGFYTGPKVPDADYKAWQDMMKKMMASKEFAA
                     LRAQRGLQPFDLTGPELDAFVKKQVGEYRKQAEEFGLVKK"
BASE COUNT         1376 a         2905 c         2944 g         1465 t
ORIGIN      
        1 gtccacgtcc atgccgttgt gcgacaggag caccaccacc cgtgcgccct ttgccctcgc
       61 ctcgtccacg gccttctgca gttccggttc ccggatgccg aaggtccagt ccggaaccat
      121 gtgccgcggg ttggcgatcg gggtgtacgg gaaggcctgg ccgacgatgg ccaccggcac
      181 gccgttgatc tgcttcagca caaacggggc gaacacggga tcgttgaagt cggcggtcgc
      241 gacgttctgc gcgacgaagt cgatcctgcc cttgaagtca ttctcgacga cctgcttcac
      301 ccgctcggcg ccgagggtga attcccagtg cgcggtcatc acgtcgacac cgagggccag
      361 gccggcgtcc accatgtcct gcccccgcgt ccacaacgcc gtggcgctgc cctgccaggt
      421 atcgccgccg tcgagcagca aggccccggg gcgcgatgcc ttgagttgct tcaccagcgt
      481 cgcgaggtgc gcgaagccgc ccaccttgcc gtaggtcctg gcggcctgcg cgaagtcgag
      541 ataggtgaag gcgtgcgcct gggcgctgtt cgcctgaacg ccgaaggcct tgagcaggct
      601 tgcccccacg aggtgcggcg ccctgccggc cgcacccgcg atgccgaggt tcacgtccgg
      661 ctcgcggaag tagatcggca ggagctgcgc gtggcagtcc gtcatgtgca gcaggctcac
      721 gttgccgaag cgcgggatgt cgtagagctt cgccccgtcc gccgcagcca gcgcctcgcg
      781 cgaggcgatc ggcagccccg cggcggcggc gatggcgaga acgttgagga actcgcggcg
      841 atccatgcag gtattctgcc ggcctcagaa ttcgagggct tcggcgatcg ccttgatgcc
      901 ggccttcgcg caagcctcgt ccgcatcccc tcccggggcg cccgagacgc cgatgccggc
      961 gaggatggag ccgcctgcct cgatcagcac gccgccgccc actgccacca cgcgcggaag
     1021 gttgcggatg cccgacgaag gctggccggg ttgcgtgatc ttcaggaggt cggcggtgtt
     1081 ggtgcggaac gagaccgccg tccacgcctt gttggtggcc gtgtccgtgg aatgcgcgcc
     1141 ggcgtagcgg tcccggacca caacctgcac cagcccggag cgatcgacga tcgcgaccga
     1201 cacctggtag ccgtcggcgc ggcacttggc gagcgccgcc tgggcggcct tgagggccgt
     1261 ctccggcgtg agtgccttca cggtgaaggt ggcgggctcc gcggcaatgg cgctgccgca
     1321 ggcgagcagg agggtcaggg cggcactggc tagtgcatta ggcatggcta atgttcctcg
     1381 gcggaaaaag ggcgggtctc cccgcccgtt ggtctcacgc cgccggctac tggttcaccg
     1441 gcgacttcgg gtcgagcagg aacgcgacga ggtccttgat ctgtgcctcg gtgagcaccc
     1501 cgttgtgccc gaagcgcggc atgttcgagc acgcggcgaa tgcctgcgaa ttgtagatct
     1561 tcgagtacgt gtacttctgg acttcgggcg agttgccgcg ggtcttcccg tagttgtaga
     1621 ggctgggacc gatgttgccg tacgagatct ccttccgggt cagctggtgg caggcgtagc
     1681 agttgccgcc ggaaggcttc ttcgggtcgt cactccactg tccgccctgg cccatctgcg
     1741 cgatcgcctc ggccttggtc cagtcgccga gcaggatgcc gttcgacggg tacttgatga
     1801 gggcctgctg gcccgcttcg aggcgttggg cgacgtcctt gggcggatgc gcgccggaaa
     1861 gggcgctgca ggtcgcctgc agctcgtcct ggtcgaggcg cgagagcgtg gcctggccgc
     1921 gcgtcttgaa gtcgcgcttc atcagggcca tggactcctt gtcgtagtcc ggggccatcg
     1981 tggcgcagcc ggcgagcgcc gccgctaccg ccgccaggga aatgatcttg gtgcgctgtt
     2041 tcatcgcggc tctcctccta tcgcttcagc gccggcgcgt tgaacacgcc gccgttcgcg
     2101 ttcttggcca ggaacagggt caacgcggac acggcgtccg acgcgtaaac cggttcgggg
     2161 aatcgctgct ggcgatagca gtcgaacagg cgatgctgca tggtgcgcac ctcgccctgc
     2221 gacacccgat agccgggcca cgcgccgtag gcacgttgcg cgtccttcgg gttgaggagg
     2281 ttgggcaggt cctgcaggcg gatgcgcttg ccttcttcgc cgtggcagga ggcgcacgcg
     2341 aaatcgtacg gaccgccgcg gtggaagaac atgcgcttgc cgatctcgta ggcttcccgt
     2401 tccttgggat gcgtggcggc gacgttcatc ttcatgccac gcgacgatcc cgtgatgtac
     2461 gcgacgatcg cctccatgtc ggacttcttg tcgttgccgg ccccgaaatg ctgcttggtg
     2521 gcctgctcga ccgtgaatcc ctgcaggctc accatgcagg tgaccagccg cgactcgagg
     2581 tcctggacct tgtcggtgtc gggaaaatac ttcggcagca cggtgtaggc gcccttgacc
     2641 acgccgggcc cgaggccgat gtcgcagcgt tcgagggacg cgcccttggg gccgcgcttc
     2701 tgcttccaca ggtcctcgcc acgggcttcc cacaactcgg cggggttgcc gtcggcgagg
     2761 gattgccggt aacgctcgat ttcctcgaca gcgctacgtt cctgagcgaa tgccccggca
     2821 gcggagagga caaatgctgc acacgcggcg cgagccgcca gttcctgggt tcgattcacg
     2881 atgctcctcc gttgcttcct tattggtccg gcgcgcatcg aggcgcgccg ggggctcacg
     2941 aaatcgtcgc ttcgtcggtg cgcttgtcgc ccttgttgtc cgtccaggtg atcgacacct
     3001 tttccccggg cttgccaccc gaaaacttga aggacaggaa cgggttcttc gaaacggccg
     3061 gcccccattc cgccgcaagc accaccttgc cattgtgcgt ggcggtgacg ctctggatga
     3121 aatgcgccgg gatgacggcg cccttcgcgt ccttgcgctg gcccgtttcc atctcgtggc
     3181 tcatcagcac cttcacttcg accttgtcgc cggacatcgc cgcccggatc ttcatcggat
     3241 ctgccatggt tttctcctga tcagccgccg cagccgccga gcgtgacttt gacttccttg
     3301 gccgtgtagt agaacttgcc gtcggccttc acgagcgcga tgacgttcga cgtctgcccc
     3361 atcttcacgc gcgtggtgat tcccgcctcg gttcccgccg ggatggtgaa gcttgccgcg
     3421 agcgcgctgg ggttcttctc caccaggagc gcgatcgatt ccgtcttcgg cagccggctc
     3481 gatatcgtga acggcacgac ggccccgttc tccgcgatgt cgggagaggc gatcgcgatc
     3541 tccttgctct ccgtggcggc cgcgccgccc atcgccttca ccgtgtcggc catgttcttc
     3601 gtctcgaacg cggccttgtt ccacgcctgc gcccaggcgc tgcccggcct gaacaggccc
     3661 gcggatgccg cgagccccat gactgccatg ctgccggtgc ccttgagcac cttcctgcga
     3721 tcgtcgttca tcactcgctc cttgagtcct cgtgtttcga tgggttgcgt ttcctggagc
     3781 cgcgcatcat gccggagcgc acgccgcgac aggcgggcgg cttcccgaca cccgaaagcc
     3841 ccgacggatc gccgggttcc ggtatttggg ggggtatgca ctcccggggt ctatccccgc
     3901 atcgggtagg gatatctacc ctgttgggta ggtggacgca ggatccgccg ccattattcc
     3961 ccgggcgcgc cccgccgggg gttccgcctg accatcggcg acaagcaata ttagtaaagt
     4021 cttcacctat tgcgctagga ttcgcccatg gcaccgaaca gggcgaaagc cgtccggcac
     4081 gacatcttcg acctgaccgg gcgcggcgag gacatcaggc aggcgagcga ggccctcaag
     4141 gcgatggcgc acccgctgcg cctcaagatc ctgtgcctcg tgggccaggg cgagttgatg
     4201 gtccaggaga tcgtcgaggc ggtcgggaca tcgcagagca acatctccca gcacctctcc
     4261 gtgatgcgcg agcgcggcct gctctcgtcg cgcaaggagg cgaacaaggt cttctaccgg
     4321 atcgaggacc cgcgcatcct caagatgatc gcgatgatgc gcgaggtgtt ctgcacgttg
     4381 taggccgtcg ccggctcggt ccccggctcc gctaggggtg cccttcccca tcgccggtcc
     4441 agcgggcagc cgcttcgtcg tcgcttgccc gtgcctcgac ccagcgctcg cccgatggca
     4501 cggattcctt cttccagaac ggcgcacggg tcttcaggaa gtccatgatg aactcgcagg
     4561 cggcgaaggc ctcgccccgg tgcgcgctgg aaacggccac caggacgatc gcctcccccg
     4621 gctccagcgg cccgacccgg tgcacgaccc gggcatcgat gacattccag cgccgcgcgg
     4681 cttcggcgca gatgtcctgc agggcccgtt ccgtcatccc cgggtaatgc tcgagggtca
     4741 tgcggcttac ctccgcgccc tcgttccggt cgcgtaccaa ccccacgaga ctggccacgg
     4801 cacccacgtc gcgccggccc tcggtgagtg ccgcgatctc gcggccggca tcgaagggcg
     4861 cctcctggac acgcacgtgc gccatcgttc agcctccggt gaccggagga aagaacgcca
     4921 cttcgtcgcc gggcttcagg ggcgtggcga ggtccgccat gcgctggttc accgccaccc
     4981 gccacgcgcg accgggggcg aatgcttccg accaacggcc atcgcgggag cgaagcctgt
     5041 cgaccacggt gcccacggtg ggcgtgcccg cgggcaatgc cacctgctcg cgggcgcaat
     5101 ccaggcgctc gcgcaggccg gcgaaataga gaagggtgat cacggggctt gccggctcgt
     5161 tgaccgaagg aggtcagtcc ggcacgacgg acaggcgggc gcgcccttcc cggccggcga
     5221 cgaattcgcg gatgaagcgc gcgacggcag ggatgtcgtc gaggccgaac tgcggcagcc
     5281 ttgtctccag cggctcgtcg gtggccacgg cgatcacgtg cgggtcgcgc ggaaagagca
     5341 gcgggcggtc gccgcccgcg cggtgcacct cgatcctcgg gatcgggtcg tgcttgtagc
     5401 cttccacgag gaccaggtcg cagggcgaga agcggcgcag caactcgtcg agcgtgggct
     5461 ccgcgtcgcc gcgcaggtcg cgcatgagtg cccagcgccg cgacgagctc accaggacct
     5521 cgttgcagcc cgcatggcgg tgccggtacg aatccttgcc cgggtggtcc acctcgaacc
     5581 ggtggtgggc gtgcttgacg agcgacaccc gcagcccttc cttcacgagg atcgggacga
     5641 gcttctcgat gagcgtggtc ttgccgctgc cgctgtagcc ggcgatgccg aggatcttca
     5701 tgtgcgtatc cgggaatcca gccggaatga tagcccaagg actgggccgg atttcctagg
     5761 cctggcgttt caggcgccgt aggagcatgg ggatcaggag gacggccgcg gccaggaccc
     5821 agagaacgag gctcaccggg ctgtggaaga gtatccctac gtgcccatcg gaaatcgaca
     5881 gggcgcggcg caggttctgc tccatcatgt cgcccagcac gaagcccagg atgagcggcg
     5941 ccatcgggaa accgagcttg cgcagggccc aaccgaagat gccgagcgcc accatgagca
     6001 cgaggtcgaa ggtggtcgcg tgcaccgcat agacgccgac gaagctcacc gccgcgatgc
     6061 ccgggacgag gatccagttg ggcacgtaaa gcagtcgcgc gaacaaaccc accatgggga
     6121 tgttcatgac gaggagcatg acgttgccga tgaacatgga cgcgatgagg ccccacacca
     6181 gctcgggctg ctgctcgaaa aggagcgggc cggggttgat gttgtagagc gtgagcgcgc
     6241 ccaccatgac ggcagtggtt ccgctgcccg gcacgcccag cgtgagcatc gggacgaagg
     6301 cgccgccggc ggcggaattg ttggccgctt cgggggctgc cacgccgcgg atgtcgcccg
     6361 tgccgaaggt gccctcgcgg tccgtgaggt tcttctcgac catgtaggtg atggcgctcg
     6421 cgatcgtcgc acccgcgcca ggcagcacgc cgatgacgaa accgatcacg ctcgagcgca
     6481 ccgtgcccca ccaggtctcg cccatctcct tgaggttgaa catcgcccgg ccggtgttgc
     6541 ggatgacgac ctggcccgcg tggctctgct cgagcatcac cagcatctcg ctcacgctga
     6601 acaggccgat cacaacgacg atgaactgga tgccgtcgga caggtgcacg tcgtcgaagg
     6661 tgaagcgata ggcccccgag cccgcgtcga tgccgacggt ggcgaggaag agcccgatga
     6721 gggcggagag cagcgtcttg acgggccggt cgcccaccat gccggccagg cagcagatgg
     6781 cgaagaccat gaggacgaag tactccgccg gcccgaaggc gagcgcccat cgcgcgagga
     6841 tcggcgcgaa aagcgcgagc ccgatggtgg cgatgatgct gcccacgaag gaactccatg
     6901 ccgagatcga gagcgccacg cccgccagcc ccttcttggc catggggtag ccgtcgaggg
     6961 cggtcatcaa cgcgccggca tcgccgggaa tgttgagcag gatcgacgag atgcgcccgc
     7021 cgtactcgca gccgatgtag acggaagcga gcaggatcag cgccgtttcc ggcggcagct
     7081 tcatggcgaa ggcgatgggc agcaggatcg ccacgccgtt gatcggcccc aggccgggca
     7141 gcagcccgac gatggtgccg atgaacgacc cgaggaacgc aaccagcagg ttcacaggcc
     7201 gcgccgcgac gatgaaaccc tgggcgaggt ggtcgaggga gtccatcagg cgagcgccca
     7261 gagccgcccg aggggcaggg tcacgtcgag gagcctgtcg aagaagtacc acaggccgcc
     7321 gccgagcgcg atcccggcga gcacgcagcc tttccaggat ccgtcgaaga gccgcccgat
     7381 caccaccgtg gcaagggccg tggcgaccac gaatcccagc cactcgaaga acatcgcgta
     7441 ggcgacgaac gccacgatca ggagtaagga cttcgcgcgc agcgcgccac gcggccagtc
     7501 cggttcacgg tccggcctga cgagaagcca ggcggcggac agcgccatca gggccgcgag
     7561 gagcagcggg aacgcgcgcg ggccgatcgg ctcgtagctg aacggggcct cgatcatccg
     7621 cgcgagccag gcgatggcgg cgcagaccac aagccagagg aaggcgaaga cgcggtcgct
     7681 caaggggccc ggtccccgct cgcgcgggga ctcctgtcga tgggaggtct tcggccgcct
     7741 acttcttgac caggccgaat tcttcggcct gcttgcggta ctcccccacc tgcttcttca
     7801 cgaatgcgtc gagctcgggg ccggtgaggt cgaagggctg caggccgcgt tgcgcgcgca
     7861 gggcggcgaa ttccttcgac gccatcatct tcttcatcat gtcctgccac gccttgtagt
     7921 cggcatcggg aaccttcggg ccagtgtaga agccgcggat gatcggccac tgcaggtcgt
     7981 agccctgctc cttggccgtg gcaatcccgg cgagcttgcc gtcgatgcgc ttgtcggcaa
     8041 gcaccgcgat catcctgatg ttgccggcct cggcctgggc ttcgtccgcg gacacatcgc
     8101 cgaacatcac ctggatgtgg ccacccatca gcgccgtggt cgcctcgccg ttgccctcga
     8161 aggacaccca gcgcatggct ttcgggtcga cgccgagctt cttggcgagg agcgccgcct
     8221 tggtccagtc ctggctgccc accgtgccgc cgccgcccat gggaaccttg gacgggtcgg
     8281 ccttgatggc ggcagccact tcggcgaggt tcttccacgg ggcgttcttg cccaccgaga
     8341 tcgagccgta gtcggtgccg acggcagcga gccaccggac gtcgttttcc gtccacttgc
     8401 cgaacttgcc ctgcgcgatg ttgaggagcg agccgcccga gaacgccacg atgacattcg
     8461 cgtcggcggc gcgctgcgcg acgaccgtat tcattgccac ggcgccgatg ccgccgggca
     8521 tgtaggtggc acgcatcggg tccttcagca gtttcaggtc gagcagcgac tgctgggcga
     8581 gcttgcaggt gaggtcgaat ccgccgccgg gcttcgcgcc gacgatgcac tcgggcttgt
     8641 cgatggtggc gaaggcggca aggctcgtca cggcggcgag ggcggaaacg
//
LOCUS       DASUEV010000204         6459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1556285, whole genome shotgun sequence.
ACCESSION   DASUEV010000204 DASUEV010000000
VERSION     DASUEV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556285"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..593)
                     /locus_tag="VFV55_06670"
     CDS             complement(162..593)
                     /locus_tag="VFV55_06670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4944020.1"
                     /translation="MKAVTTRTGMFVSRQDSLRDARGFLERFCSAAGVARQPCLKLNL
                     VVEELFLNTVKHGHRGGSDAPVWITLAAGDGAIRMTYEDRAPPFNPFAEATREMLEAL
                     AGTRREGGLGVLLAHGLATETDYAYLYGRNRIRLTIATPVE"
     gene            660..1118
                     /locus_tag="VFV55_06675"
     CDS             660..1118
                     /locus_tag="VFV55_06675"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX4944021.1"
                     /translation="MRKFWILVSALAFAASAAANDIGQVKVAKGTVQVEREGKRVAAA
                     VGMPVRTADVLVTGADGSAGITFSDNSLVSIGPNSVFAIDKYRFDTTTHAGEFEGSLR
                     KGRLAAVSGKMVKQTPESMRIRTPSSVMAVRGTDFVVQVDEPAAPPAAAR"
     gene            1132..1740
                     /locus_tag="VFV55_06680"
     CDS             1132..1740
                     /locus_tag="VFV55_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4944022.1"
                     /translation="MRLAAAVLVLAVAGCAEAPKAPAPVGAELVAVIPGPDGHVGAVV
                     VHSGGGAQVLDQAFSARRVKPDGSTEAALLTSAEVTRAFGPTIEALPGRPASFVLYFL
                     EGKDELTPASRVELDKVFAELKRRPAPDIVVTGHTDTVGNLAFNDKLSLARAERLREM
                     MVGLGIPPERIQATGRGKRELLVPTDDNTAEPRNRRVEISVR"
     gene            complement(1767..3782)
                     /locus_tag="VFV55_06685"
     CDS             complement(1767..3782)
                     /locus_tag="VFV55_06685"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX4944023.1"
                     /translation="MSLLLAVSPLPVERFRNVVFDGWQRLMPRERLSDQVVVVVIDEK
                     ALALHGQWPWPRDRVAELVSRIAKARPLVLGMDIFFPEPDRYSPAMMAARLEDRAPDI
                     AARLKGMPSNDALLGAALGTIPSVLGVAALESRDPRFAGPPGAPPVRFLGGAGEGLRT
                     YPGHLRNVPEIDAGAAGHGLYSVDLEAGVARRLQMIGRFDEAAVPALAVEMIRVALHA
                     PLTVAPSLGGTIELRFGDYAVPAQRDGRAFVHYGYSAPARFVSAAAILDGSFDASPLE
                     GKLVLVGFTGLGLVDLVATPLGETAFGVENHAQAIEAILEGRFLRRVELASRIEAALL
                     VAFGLGLFALIPRSNAQRSAALLVAGIAILLAAGAAAFRYAGLLFDPAGPALGAIAVF
                     GVMQAGSLAEAQRQRQQLREQAARMSGELDAARRIQMGLLPDPREALGSERRADLAAL
                     LEPARTVGGDFYDCFMLDDRRLFFLVADVSGKGLPASLFMASVKSILKSAALTVDGPV
                     GAILSRAQDEIHRENPESLFVTVLAGALDLETGELECANAGHEPVWSHQPRGSPERLD
                     LSGGPPLCVLEAYPYPSTRRALARGEWLLVLSDGATEAMNAAGEFFGVERLRTSLAWL
                     GDETDAVAIVSRVRDDVRRFSGAAEPADDLTLLAIRWNGPEGPASGC"
     gene            complement(3830..4792)
                     /locus_tag="VFV55_06690"
     CDS             complement(3830..4792)
                     /locus_tag="VFV55_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4944024.1"
                     /translation="MGMRIRFWGPRGSIPVALTSFDIRDRLARALVEASGRSFASYEE
                     AHAFAATELDFSITHTFGGHTPCVELDLGGDEYFVCDMGSGARPFGVHVLAKQARRPA
                     TVNVFMSHVHWDHIMGFPFFGPAYVPGTKIRIHSCHDVCEHAFRLQQAAPCFPVDFSQ
                     LLASIEFVKLVPDQPSVVSGLTVTPHLQLHSGDSYGFRFEKDGKRVVYSTDSEHKLEN
                     RAEAESFAAFFRNADVVIFDAMYALADAISVKADWGHSSNIVGVELCQMAHARHLVLF
                     HHEPANDDATLEGLLKEARRFEELTRSGEPLKVSAAFDGLEIEP"
     gene            complement(4795..5202)
                     /locus_tag="VFV55_06695"
     CDS             complement(4795..5202)
                     /locus_tag="VFV55_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4944025.1"
                     /translation="MSLQARRYANALVLCFTGRLDQDNCDAFRADLTAQVERSACDGG
                     AVILDLSGLEYVSSAGLRCFMLASRQANARQARILVAALQPMVAEIFAISHFDMVIQV
                     LPTVREALAAASREAAEAFDQARAASAEERAGE"
     gene            5297..>6459
                     /locus_tag="VFV55_06700"
     CDS             5297..>6459
                     /locus_tag="VFV55_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX4944026.1"
                     /translation="MHTTPHSPDAPRLLSRRQALAALALPVTLPLAGCWDTFGLLNPC
                     ANRGDPGHRIDPAISAFVWAGLDARNVLDMHVHLSGEGTAPGDPWVNPAMRTLAHPFS
                     YTHFALMANAACLGNDPANWSRRYVSRLVALADEFRPGARFLLLALDGYHGADGRLDR
                     ARTVFMVPDEYAARVARGNPGRFLWAASVHPYREDALERLRSAAENGARAVKWIPYFM
                     GIDPANPRCRPFYRQIARLGLPLITHGGWEHELMEDGQQDLGNPLRLRAALEEGATVI
                     MAHCAIQGDFADLDRPMSRTPKPSFELFARLLDAPEYRSRLYGDLSAVVQGGRQPGAL
                     ARLLSTFEWHPRLLNGSDYPGPGVVAVMGMKDLVEEGVLAAKDAERIWHIQKH"
BASE COUNT         1017 a         2251 c         2266 g          925 t
ORIGIN      
        1 gactttccgg gcacccatcc gacggtctcg cttgcgtgcc caggattgcg ctcgactctt
       61 cccggcgcct ccctaacaag cacccaggat tgcgctcgac tttccccgac gcctcccaaa
      121 acacccccag gactgcgatc gacgttcgtc gacgcctcga gctattcaac gggggtggcg
      181 atggtgagcc ggatgcgatt gcgcccgtac aggtaggcgt agtcggtttc ggtggcgagg
      241 ccgtgcgcga ggagcacgcc cagccctcct tcgcgccgcg tgccggccag cgcctcgagc
      301 atctcgcgcg tggcctcggc gaagggattg aagggcggcg cgcggtcttc gtaggtcatg
      361 cggatggcgc catctcctgc cgcgagcgtg atccagacgg gtgcgtcgga cccgccgcga
      421 tggccatgct tgacggtgtt gaggaagagc tcctcgacga ccaggttgag tttcaggcac
      481 ggctgccggg ccacgccggc ggcgctgcag aagcgctcga gaaagccgcg cgcgtcccgc
      541 agggaatcct gccgggagac gaacatgccg gttcgcgtcg tgactgcctt cacgggatcg
      601 ccttttcggg cattatagat tgggtccatt tccggtcccg atcgctcagg aagacactca
      661 tgcggaaatt ctggattctc gtttcggccc tggcgtttgc cgcatccgcg gccgcgaacg
      721 acatcggcca ggtcaaggtc gccaaaggca ccgtgcaggt cgaacgcgag ggcaagcgcg
      781 tggcggccgc ggtgggcatg cccgtgcgca cggcggacgt gctggtcacc ggcgccgatg
      841 gctcggccgg catcaccttt tccgacaaca gcctcgtgtc catcggtccg aacagcgtct
      901 tcgccatcga caagtatcgc ttcgatacca ccacccacgc gggcgaattc gaggggtcgc
      961 tgcgcaaggg caggctcgcg gcggtgtcgg gcaagatggt caagcagacg cccgagtcga
     1021 tgaggatccg cacgccctcc tccgtgatgg ccgtgcgcgg caccgatttc gtggtgcagg
     1081 tcgacgagcc tgccgcgcca cccgcagccg cacgctgagc ccgcagacgc gatgcgcctt
     1141 gcggccgctg tcctggtgct cgccgtcgcg ggatgcgcgg aggccccgaa ggctccggcg
     1201 ccggtcggcg ccgaactggt tgccgtgatc cccggcccgg acggccatgt cggcgcagtc
     1261 gtggtgcatt ccggcggtgg cgcgcaggtg ctggaccagg ccttttccgc ccggcgcgtg
     1321 aaacccgacg gcagcaccga ggcggcgctc ctgacctccg cggaagtgac ccgcgcattc
     1381 ggcccgacga tcgaggcgct gccgggccgg cccgcgtcct tcgtgctcta tttcctcgag
     1441 ggcaaggacg agctcacgcc ggcctcgcgc gtggagctgg acaaggtctt tgccgagctg
     1501 aagcgccgcc cggcgcccga catcgtggtg accggccaca ccgacaccgt cggcaacctc
     1561 gccttcaacg acaagctgtc gctcgcgcgg gccgagcgcc tgcgcgagat gatggtcggc
     1621 cttggaatcc cgccggagcg catccaggcc accgggcgcg gcaagcggga gctcctcgtg
     1681 ccgacggacg acaacaccgc ggagccgcgc aatcgccgcg tcgagatcag cgtccgctga
     1741 ttgcgcccgg catgcgccga cggcgctcaa cacccgctgg ccgggccttc gggcccgttc
     1801 cagcggatcg cgaggagtgt aaggtcgtcg gcgggttccg ccgccccgga gaaccgccgc
     1861 acgtcgtcgc gcacgcggct cacgatcgcg accgcgtcgg tttcgtctcc cagccacgcg
     1921 agcgaggtgc gcaggcgctc cacgccgaag aactccccgg cagcgttcat ggcctccgtg
     1981 gcgccgtcgg aaagcacgag cagccattcc ccgcgcgcga gcgctcgccg ggtcgagggg
     2041 tacgggtacg cttcgagaac gcacaaaggc ggccccccgg aaagatccag gcgctccggg
     2101 ctcccgcggg gctggtgcga ccacaccggc tcgtgcccgg cgttggcgca ctcgagctcc
     2161 cccgtttcca ggtccagcgc gcccgccagc accgtgacga agagtgattc cgggttttcc
     2221 cggtggatct cgtcctgcgc ccgggagagg atggcgccga cgggaccgtc caccgtgagc
     2281 gccgcgctct tgaggatcga cttgaccgag gccatgaaaa gcgaggccgg aagccccttg
     2341 cccgagacgt cggcgaccag gaagaagagc cgccggtcat cgagcatgaa gcagtcgtag
     2401 aagtcgccgc ccaccgtgcg cgcgggttcg aggagggcgg ccaggtcggc gcgccgctcg
     2461 gagccaaggg cctcgcgcgg gtccgggagc agccccatct ggatgcgcct ggcggcatcg
     2521 agctcaccgg acatgcgcgc ggcctgttcg cgcaattgct ggcgctggcg ctgcgcttcg
     2581 gccagcgatc cggcctgcat cacgccgaag acggcgatcg ccccgagcgc cgggcccgcc
     2641 gggtcgaaga gaaggcctgc gtagcggaac gcggccgcgc ccgcagcgag caggatcgcg
     2701 atgcccgcga cgagcagggc cgccgagcgc tgggcgttcg agcgcgggat gagcgcgaag
     2761 agaccgaggc cgaaggccac gaggagcgcc gcctcgatcc gcgacgcgag ttcgacgcga
     2821 cgcaggaacc ggccctcgag aattgcctcg atcgcctgcg cgtggttctc cacgccgaac
     2881 gccgtttcgc ccagcggcgt ggccaccagg tcgacgagcc cgaggccggt gaagccgacc
     2941 aggaccagct tgccctcgag cggcgatgcg tcgaaggatc cgtcgaggat cgcggcggcg
     3001 gacacgaagc gcgccggggc cgagtaacca tagtggacga aagcccggcc gtcgcgctgg
     3061 gcgggaactg cgtagtcgcc gaagcgcagc tcgatggtcc cgccgagcga cggcgcgacg
     3121 gtgagcggcg cgtgcaaggc gacgcggatc atctccaccg cgagagcggg caccgcggcc
     3181 tcgtcgaagc ggccgatcat ctgcaggcgt cgcgccacgc cggcctcgag gtccacgctg
     3241 tagagcccgt ggccggccgc gccggcgtcg atctcgggca cgttgcgcag gtgcccggga
     3301 taggtgcgaa ggccctcgcc cgccccgccc aggaagcgaa ccggcggcgc gcccggcggg
     3361 ccggcgaatc gcgggtcgcg cgactcgagc gccgcaacac ccagcaccga cgggatcgtg
     3421 cccagcgccg cgcccagcag cgcgtcgttc gaaggcatcc ccttcagcct ggccgcgatg
     3481 tccggcgccc ggtcctcgag gcgggcagcc atcatcgccg gcgaatagcg gtccggttcc
     3541 gggaagaaga tgtccatccc cagcacgagc gggcgggcct tcgcgatgcg cgagacgagt
     3601 tcggcgacgc gatcgcgcgg ccagggccac tgcccgtgga gggcaagcgc cttctcgtcg
     3661 atcaccacca ccacgacctg gtcggacagg cgttcgcgag gcatgaggcg ctgccagccg
     3721 tcgaaaacga cgttgcggaa gcgctcgacc ggcagcggcg agacggcgag cagcagggac
     3781 aacgcggcca gcaggacgaa cgcggcgacg cgaaacgcgc cggaggcgct cagggctcga
     3841 tctccagccc gtcgaatgcc gccgagacct tgagcggctc gcccgagcgc gtgagctcct
     3901 cgaagcggcg cgcctccttg agcagcccct cgagcgtggc atcgtcgttg gccggctcgt
     3961 gatggaagag aacgaggtgg cgcgcgtgcg ccatctggca gagctcgacg cccacgatgt
     4021 tgctcgagtg cccccagtcg gccttgacgc tgatcgcgtc cgccagcgcg tacatggcgt
     4081 cgaagatcac cacgtcggcg ttgcggaaga aggccgcgaa gctttcggcc tcggcgcggt
     4141 tctcgagctt gtgctcggag tcggtcgagt acaccacgcg cttgccgtcc ttctcgaagc
     4201 ggaagccgta ggagtctccc gagtgcagct gcaggtgcgg cgtgaccgtc aggccggaga
     4261 cgacgctggg ctggtcgggc acgagcttca cgaactcgat ggaggcgagg agctgcgaga
     4321 agtccacggg gaagcacggt gcggcctgct gcaggcggaa agcgtgctcg cagacgtcgt
     4381 ggcaactgtg gatgcggatc ttcgtgcccg gcacgtaagc cgggccgaag aagggaaagc
     4441 ccatgatgtg atcccagtgc acgtgcgaca tgaacacgtt caccgtggcg gggcgccgcg
     4501 cctgcttggc gagcacgtgc acgccgaagg gacgcgcgcc gctgcccatg tcgcaaacga
     4561 agtattcgtc gccgcccagg tcgagctcga cgcacggcgt gtgcccgccg aaggtgtggg
     4621 tgatggagaa gtccagctcc gtcgcggcga aagcgtgcgc ctcctcgtag gaggcgaagc
     4681 tgcgtcccga tgcctccacg agggcgcgcg cgagcctgtc gcggatgtcg aacgaggtga
     4741 gggccaccgg aatggagccc cggggtcccc agaagcggat gcgcatgccc atgactattc
     4801 tccggctcgt tcctcggcgc tggcggcccg ggcctggtcg aaggcctccg cagcctcgcg
     4861 ggaggcggcc gcgagcgcct cacgcaccgt gggaaggacc tggatcacca tgtcgaaatg
     4921 gctgatcgcg aagatctcgg cgaccatcgg ctgcagggcg gccaccagga tacgggcctg
     4981 tcgcgcattc gcctggcgcg acgcaagcat gaagcagcgc agtccggcgc tcgaaacgta
     5041 ctcgaggccg gacaggtcga ggatcaccgc gcccccatcg cacgcggatc gttcgacctg
     5101 cgccgtgagg tcggcacgga aggcgtcgca gttgtcctgg tccagcctgc cggtgaagca
     5161 caggaccagt gcattcgcgt atctgcgtgc ctggagagac atttgttctg gttatggggc
     5221 ggttccgtca cctattgtcg gggacgcccg ggggaagggc aagcgggccg tggcttgcgc
     5281 cgccacgtcg cgtaacatgc acacgacgcc ccattctccc gacgccccgc gattgctttc
     5341 cagacgacag gcgctcgccg ccctcgccct tccggtcacg ttgccgctcg ccgggtgctg
     5401 ggacacgttc ggcctgctca atccgtgcgc caaccgtggc gaccccggcc accggatcga
     5461 tccggcgatc agcgcgttcg tgtgggccgg gctggatgcc cgcaacgtcc tcgacatgca
     5521 cgtccatctc tccggcgagg gcaccgcacc gggcgatccc tgggtcaacc cggccatgcg
     5581 tacgctcgcg cacccgttct cctacacgca ctttgcgctg atggccaacg ccgcctgtct
     5641 cggcaacgac ccggccaact ggagccggcg ttacgtgagc cgcctcgtgg cattggccga
     5701 cgagttccgg cccggcgcgc gattcctgct gctcgccctc gacggctacc acggcgccga
     5761 cggccggctg gaccgcgccc gcaccgtgtt catggttccc gacgagtacg cggcgcgcgt
     5821 ggcccgcggc aatccgggtc gcttcctttg ggcggcctcc gtccacccct atcgcgagga
     5881 cgccctcgag cgcctgcgct cggccgccga aaacggagcg cgggcggtca agtggatccc
     5941 ctacttcatg ggaatcgatc ccgcgaatcc gcgctgccgc ccgttctacc ggcagatcgc
     6001 aaggctcggc ctgccgctca tcacccacgg cgggtgggaa cacgaactga tggaagacgg
     6061 gcagcaggac ctgggcaatc ccctgcgcct gcgcgccgcc ctggaggaag gcgccacggt
     6121 gatcatggcg cattgcgcga tccagggcga tttcgccgac ctggaccggc cgatgtcgcg
     6181 cacgccgaaa ccgagcttcg agctgttcgc gcgcctgctc gacgcgcccg aataccgcag
     6241 ccggctctac ggggacctgt ccgcggtcgt gcaggggggg cggcagcccg gcgcgctggc
     6301 gcgcctcctc tccacattcg agtggcaccc gcgcctgctc aacggctcgg actaccccgg
     6361 ccccggcgtc gtcgccgtca tggggatgaa ggacctggtg gaagaggggg tgctcgcggc
     6421 gaaggatgcc gagcggatct ggcacatcca gaagcacaa
//
LOCUS       DASUEV010000205         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1306142, whole genome shotgun sequence.
ACCESSION   DASUEV010000205 DASUEV010000000
VERSION     DASUEV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306142"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1052)
                     /locus_tag="VFV55_06705"
     CDS             complement(144..1052)
                     /locus_tag="VFV55_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX4944027.1"
                     /translation="MSARSPTFYILFSVATSLITMALKTGAYLLTGSVGLLSDALESG
                     VNLAAALVALATLSYAHSGEDDDHNFGHEKAEYFSSGIEGALIFVAAAAIIWSAVPRL
                     ASPRPLEQVGVGLALSVLAAGANLLCGWFLLKGAREHRSITLEADAHHLLTDVWTTVG
                     VIAGVAVVHFTGWLILDPVIAIAVAVQILWTGWSLIRRSFDGLMDRSIPADERKRIAA
                     ILEQVKASGGDFHRLRTRVSGKKSFVDVHILVPGSMSVHDGHDLAHRLEKEIEAAVPH
                     VEALTHIEPLEDPRSWDDPDNPLAGS"
     gene            complement(1049..1990)
                     /locus_tag="VFV55_06710"
     CDS             complement(1049..1990)
                     /locus_tag="VFV55_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4944028.1"
                     /translation="MKNATLRQLRVFESAARHLSLTRAAGELHLSAPAVSIQVKNLEG
                     HAGAALFERGGRRLRLTQEGEEVLLHVRDILGHIRGAEEAIAELDSLSRGLLDVAVIN
                     AGDHFFPWIVAAFRQMHPGIRVRLTVQNRDELLARLAACQTDLAVMSHPPTEPGHVAT
                     AFAPHPHVIAAAPSHPLARKRRIPLARIAAEPILTREPGAATRLAMDQVFAEAGVVPR
                     IEMEIASNETIKQAAAAGLGVGFLSAHALRQELALGSLVILDVEGFPVMRQWYVVHRL
                     DRRLPRIAQAFERFVLENGERLIGRPVVKPGRAGKGR"
     gene            2084..>2304
                     /locus_tag="VFV55_06715"
     CDS             2084..>2304
                     /locus_tag="VFV55_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944029.1"
                     /translation="MDTIEPTRSGFAGCDALNAAFWDLGLRFQWDAATWGVLVQMEDF
                     QAQIAYYLEHWQPHLLAVYEPAFLARMVE"
BASE COUNT          392 a          780 c          769 g          363 t
ORIGIN      
        1 tggccccgac tttttgggac ccatccgacg gtcacgtttg cgtgcccagg acgccgatag
       61 acttgcccca tccccggcct acctctcatt cgccggtcac attcaggtgc tcacgacgca
      121 gagagacttg cccaatcccc cgcctaactc ccggccaggg gattgtcggg gtcgtcccag
      181 cttcgcgggt cttcgagggg ctcgatatgc gtcagcgcct cgacgtgcgg gacagcggcc
      241 tcgatctcct tctcgaggcg gtgggcgagg tcatggccgt catgcacgct catggacccg
      301 ggcacgagaa tgtgcacgtc gacgaagctc ttcttgcccg acacgcgcgt gcgcagtcgg
      361 tggaagtcgc cgccgctggc cttgacctgc tcgagaatgg ccgcgatgcg cttgcgctcg
      421 tcggcgggaa tcgagcgatc catgagcccg tcgaaagagc ggcggatgag ggaccatccc
      481 gtccacagga tctgcaccgc caccgcgatg gcgatcaccg ggtcgaggat cagccacccc
      541 gtgaaatgca cgacggccac gcccgcgatc acgcccaccg tggtccacac gtcggtgagc
      601 aggtggtgcg cgtcggcctc tagggtgatc gagcggtgct cgcgcgcacc cttgagcagg
      661 aaccagccgc acagcaggtt cgcgcccgcg gcgagaacgg aaagggccag gcccacgccc
      721 acctgctcga ggggcctggg cgaggcgagc cgcgggaccg cgctccagat gatcgccgcc
      781 gccgcgacga agatgagcgc gccctcgatg ccgctggaaa agtactccgc cttctcgtgg
      841 ccgaagttgt ggtcgtcgtc ctcgccggaa tgcgcgtacg aaagcgtggc cagcgccacc
      901 agcgcggccg cgaggttgac ccccgattcc agcgcatccg agagcagccc gaccgaaccg
      961 gtgagcaggt aggcgcccgt cttgagcgcc atcgtgatga gcgaagtcgc gaccgaaaac
     1021 aggatgtaga aggtggggga acgcgcgctc atcgcccctt ccccgcgcgg ccgggcttca
     1081 ccacggggcg accgatgagg cgctcgccgt tttccagcac gaagcgctcg aaggcctgcg
     1141 cgatcctcgg aagccgacga tcgagccggt gcaccacgta ccattgccgc attacgggga
     1201 aaccctccac gtccaggatt acgaggctac cgagcgcgag ctcctggcgc aatgcgtgcg
     1261 ccgaaagaaa gccgacgccc agccccgccg cagccgcctg cttgatggtc tcgttgcttg
     1321 cgatttccat ttcgattcgc ggtaccacgc cggcctccgc gaagacctgg tccatcgcca
     1381 gccgcgttgc cgcgccgggc tcgcgcgtga gtatgggttc cgccgcgatg cgcgcgagcg
     1441 gaatgcggcg ttttcgcgcc aggggatggc tgggcgccgc cgcgatcacg tgcgggtgag
     1501 gggcgaacgc cgtcgcgaca tgcccgggct ccgtgggcgg atggctcatc accgcgaggt
     1561 cggtctggca ggccgccagc cgcgccagca gttcgtcgcg attctgcacc gtgaggcgaa
     1621 cgcggatgcc ggggtgcatc tgcctgaacg cggccacgat ccacgggaag aaatggtcgc
     1681 ccgcgttgat gaccgccacg tcgagcaggc cccgggagag cgaatcgagc tcggcgatgg
     1741 cctcctcggc gccgcggatg tggccgagga tatcgcgcac gtgcaggagc acttcctcgc
     1801 cttcctgcgt gagacgcagg cgcctgccgc cgcgctcgaa gagggccgcc ccggcatggc
     1861 cctcgagatt cttcacctgg atcgaaacgg ccggcgcgct gagatgcagc tccccggccg
     1921 cacgcgtgag cgacaggtgc cgggcggcgc tttcgaacac gcgcaactgg cggagcgtgg
     1981 cgttcttcat taaacgatcc cttaaccgaa gccaaagatc attttacttt cacttaacac
     2041 tttccgcgag taccttgggc tcgacgacaa acggaggggc accatggaca cgattgaacc
     2101 gacccgcagc ggattcgccg gatgcgacgc attgaacgcg gccttctggg acctggggct
     2161 gcgcttccag tgggatgccg cgacctgggg cgtgctcgtg cagatggaag acttccaggc
     2221 gcagatcgcc tactacctgg agcactggca gccgcacctg ctggcggtct acgagccggc
     2281 cttcctcgcg cgaatggtcg agag
//
LOCUS       DASUEV010000206         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1436982, whole genome shotgun sequence.
ACCESSION   DASUEV010000206 DASUEV010000000
VERSION     DASUEV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436982"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..686)
                     /gene="ispF"
                     /locus_tag="VFV55_06720"
     CDS             complement(204..686)
                     /gene="ispF"
                     /locus_tag="VFV55_06720"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX4944030.1"
                     /translation="MSALRIGQGFDVHQLVAGRKLVIGGVDIAHDKGLLGHSDADVLL
                     HAICDALLGAAALGDIGRHFPDSDPRYEGIDSRELLRRVAGLVREEGFVPGNIDATII
                     AEAPRMAPHIPAMVAHIAADVGIDEDCVNVKATTTERLGFTGRGEGIAAQAVCVLESR
                     "
     gene            complement(715..1413)
                     /gene="ispD"
                     /locus_tag="VFV55_06725"
     CDS             complement(715..1413)
                     /gene="ispD"
                     /locus_tag="VFV55_06725"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX4944031.1"
                     /translation="MPKIFALVPAAGQGTRLGDALPKQYLPLAGRPMMFHSLAALAAV
                     GRIDHVIVILSPLDRHWGGHDRGALPGKVEAIFAGGDSRAESVANALVLLEGRAAAGD
                     WILVHDAARPCLEAPLVERFLDEVGDDPVGGLLAMPLADTLKRAGESMRVDETIPREG
                     LWRAQTPQMFRYELLKRGLARKPGATDESQAVESLGQMPRIVQGAADNIKVTFAEDLP
                     LAGMILVRQSGVSP"
     gene            1514..2926
                     /gene="mltF"
                     /locus_tag="VFV55_06730"
     CDS             1514..2926
                     /gene="mltF"
                     /locus_tag="VFV55_06730"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEX4944032.1"
                     /translation="MTARILLGILACLVAGCEVATKPVMPLSEGGKLVVLTINGPATW
                     YEDAQGDPGGFEHDLVDLFAKDLGVPLEYIFVASPEKAEKALSEKRAHLAAALLPRNF
                     DLPGGLAWGPSYRSAQYQIVWPLTFPKPRTLSDFAGRRVGVIGDPLSNRMLAAQTKIS
                     IPLERLPSDTLPEELLEQVTEGHLEAALIDSARFTVERKHFPQLDVAFDVGKPVDFAW
                     RVGTVDQKLLLDRMKVFFAKLEKDGTLRRLADRHFAHAARISSIDAGTLIERINTMLP
                     RLKPYFLEAERISGFDWRLIAAIGYQESHWDPRATSPTGVRGLMMLTEDTADRLKVKD
                     RLDPRESILGGARYLGVMADAIPPRIREPDRTWLALAAYNIGIGHLEDARILAQRKGL
                     SPDTWQDVKLALPGIADPATYEQLKHGFARGHEAMQFVDNVRNYQDILARVEPRDDRL
                     APRPPPALSQGSTKGSMAAR"
     gene            complement(2890..>3201)
                     /locus_tag="VFV55_06735"
     CDS             complement(2890..>3201)
                     /locus_tag="VFV55_06735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX4944033.1"
                     /translation="VRDTDPDYVVPGGESRRQLFERVRDAFESLARASDGRCIAVVCH
                     GGVLAALYRHVHGIPVGTAQPIPIPNASYNALVCDGGRWEVEAWADTAHLAAIEPFVE
                     P"
BASE COUNT          513 a         1148 c         1049 g          491 t
ORIGIN      
        1 gctttgaccg attgaatccg ccccagatga aactgctgcc aacatcgact ccggcaacac
       61 ctcgacatag gttcgatccg tgacgcccga cgcaacttga cctggccaaa aaccgaaaaa
      121 aacaccaacc acaccctgct ccggcatcgc tccgggaagc atccctgacg cggcgtggcg
      181 tcaccaggat tgcgctgcgc gggtcagcgc gattccagca cgcagaccgc ctgtgcggcg
      241 atgccctccc cgcggccggt gaagccgagc ctctcggtgg tggtggcctt cacgttgacg
      301 cagtcctcgt cgatgccgac gtcggcggcg atgtgggcga ccatggccgg gatgtggggt
      361 gccatgcgcg gcgcctcggc gatgatcgtc gcatcgatgt ttcccggcac gaatccctct
      421 tcgcgcacca gccccgccac gcggcgcagc agctcgcggc tgtcgatgcc ctcgtagcgc
      481 gggtcgctgt cgggaaagtg ccggccgatg tcgccgagcg ccgcggcgcc cagcagcgcg
      541 tcgcagatcg cgtgcaggag aacgtccgcg tcgctgtggc ccagcagccc cttgtcgtgc
      601 gcgatgtcca cgcccccgat cacgagcttg cggcccgcga caagctggtg gacgtcgaac
      661 ccctggccga tgcgcaatgc gctcatgtct atcccgcctc cccgcctgtt cgcctcatgg
      721 ggacacgccc gattgccgga cgaggatcat cccggcgagc ggcaggtcct cggcgaaagt
      781 caccttgatg ttgtcggccg ctccctggac tatgcgcggc atctgcccga gggactcgac
      841 cgcctgggac tcgtcggtcg cgccgggctt gcgcgcaagg ccgcgcttga gtagctcgta
      901 gcggaacatc tgcggtgtct gcgcgcgcca caggccctcg cgcggaatcg tctcgtccac
      961 gcgcatcgat tcccccgcgc gcttgagcgt atcggccagc ggcatggcga ggagcccgcc
     1021 caccgggtcg tcacccactt cgtcgaggaa tcgctcgacg agcggcgctt cgaggcaggg
     1081 gcgcgcggca tcgtgcacga ggatccagtc gccggccgcc gcccgtccct cgaggaggac
     1141 gagcgcattg gccacgctct ctgcgcgcga gtcgccaccc gcgaatatcg cctcgacctt
     1201 tccgggcagg gcgccgcggt cgtgtccgcc ccagtggcgg tcgagcggcg aaaggatgac
     1261 gatcacgtgg tcgatcctgc cgaccgcggc cagtgccgcg agcgagtgga acatcatcgg
     1321 gcgcccggcg agcggcaggt actgcttggg cagtgcgtct cccaggcgcg tgccctgccc
     1381 ggccgcgggc acgagtgcga agatcttcgg catcgtcggt atccggcgac gattatcgcc
     1441 gatttcggcg gcgcaacgga ttttgcggcg gcgcaagttg aatcgacgcc gccggcaggt
     1501 cacacttcgg gccatgacag cgcggatact cctcggcatc ctcgcgtgcc tcgtggcggg
     1561 atgcgaagtc gccaccaagc cggtcatgcc gctatccgag gggggcaagc tcgtggtcct
     1621 caccatcaac gggcccgcaa cgtggtacga ggatgcgcag ggcgaccccg gcggcttcga
     1681 acacgatctc gtcgatctct tcgcgaagga cctcggcgtt cccctcgagt acatcttcgt
     1741 ggcgagcccc gaaaaggcgg agaaggcgct ctcggaaaaa cgcgcgcacc tcgcggccgc
     1801 gctgctgccg cgaaacttcg acctgccggg cggcctcgcc tggggcccgt cctaccgttc
     1861 agcgcagtac cagatcgtgt ggcccttgac gttcccgaaa ccgcgcacgc tctccgattt
     1921 cgcgggccgg cgcgtgggcg tgatcggcga tcccctgtcc aaccggatgc tcgccgcgca
     1981 gacgaagatc tccatcccgc tcgagcgcct gccctccgac accttgcccg aggagttgct
     2041 cgagcaggtg accgaaggtc atctcgaggc ggcgctcatc gactccgccc gattcaccgt
     2101 cgagcgcaag catttcccgc aactcgacgt cgcgttcgac gtcggcaagc ccgtcgattt
     2161 cgcgtggcgc gtgggcaccg tcgaccagaa gctgctcctc gaccgcatga aggtcttctt
     2221 cgcgaagctc gagaaggacg gcacgctccg gcgcctggcc gatcgccact tcgcgcacgc
     2281 cgcgcgcatc tcctccatcg acgcgggaac cctcatcgag cgcatcaaca cgatgctgcc
     2341 gcgactgaag ccgtacttcc tcgaggccga acgcatctcc ggattcgact ggcgactcat
     2401 cgcggccatc ggctaccagg agtcgcactg ggacccgcgc gccacgtcgc ccacgggcgt
     2461 gcgcggcctc atgatgctca ccgaggacac ggccgaccgg ctcaaggtga aggatcgcct
     2521 cgatccccgc gagagcatcc tcggcggggc gcgctacctc ggggtgatgg ccgacgcgat
     2581 accgccccgg atccgtgagc ccgaccgcac ctggcttgcg ctggcggcct acaacatcgg
     2641 catcggccac ctcgaggacg cgcgcatcct cgcgcagagg aaggggctgt cgcccgacac
     2701 gtggcaggac gtgaagctgg cgctcccggg aattgccgac ccggcgacct acgagcagct
     2761 caagcacggg ttcgcgcgcg gccacgaggc gatgcaattc gtggacaacg tgcgcaatta
     2821 ccaggacatc ctcgcccgcg tggagcctcg cgatgatcgg ctcgcgcccc ggccgccgcc
     2881 cgccctgtct cagggctcca cgaagggctc gatggccgcg aggtgagcgg tgtcggccca
     2941 tgcctccacc tcccagcgcc cgccgtcgca cacgagcgcg ttgtaggagg cattggggat
     3001 agggataggc tgcgcggtcc cgacggggat cccgtggaca tggcggtaga gcgcggcaag
     3061 aacgccgcca tggcagacca cggcaatgca ccggccatcg gaagcgcgcg ccagcgactc
     3121 gaaggcgtcg cgcacgcgct cgaaaagctg ccgccggctt tcgccgccgg gcaccacgta
     3181 gtccgggtcg gtgtcgcgca c
//
LOCUS       DASUEV010000207         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_68217, whole genome shotgun sequence.
ACCESSION   DASUEV010000207 DASUEV010000000
VERSION     DASUEV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68217"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1123
                     /locus_tag="VFV55_06740"
     CDS             71..1123
                     /locus_tag="VFV55_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4944034.1"
                     /translation="MGERYAAAALKGFARALLEKAGMEPPKAGDVALILVEGDLLGHD
                     THGLALLAPYLRELEAGTMAKSGQPEVLKRRGATETWDGKRLPGPWLTLRALDRAMAM
                     AREFGTGTVVIRRSHHIACLAAYLRRLTEQGLVVQILCSDANSASVAPFGGTQAVFTP
                     NPIAIGFPTVGDPVLVDISASLATNALTNRLRAQGAKLPHAWLLDGQGQPSDDPATLA
                     AEPKGTILPLGGLEAGHKGYGLALLVEAMTGGLAGFGRADAKQGWGATVFVQVLDPEA
                     FAGLADFKRQTQWVADACRANAPRPGLPPVRLPGDGGLARRQEQLAGGIALHPDILPA
                     IAPWAEKLGVPLPAAA"
     gene            complement(1130..2038)
                     /locus_tag="VFV55_06745"
     CDS             complement(1130..2038)
                     /locus_tag="VFV55_06745"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4944035.1"
                     /translation="MAEAKPRIGFIGVGMMGHGMARNLQAKGFPLAILVHRNRERAAD
                     LLAAGAREAKTPAELAATSDVVILCVSGSPQVEENVLGAQGIASAARDGMIVIDTSTA
                     EPGSTARLRDALAAKGVRFVDAPLARTPKEAEEGRLNTMVGADDATFALIRPVLAAFC
                     ENIFHVGPPGSGHTLKLVNNFMALTIAASIAEAFAVAAKAGVPLDMLVEVVSQGALNS
                     NIFQMMGRGAVQGDLTRMKFSIENAAKDLRYYTRLAESLPVATFVGEAVHQSFSQAVA
                     MGLGGKLVASMIEAQERIYGLEIVKR"
     gene            2107..2919
                     /gene="nudC"
                     /locus_tag="VFV55_06750"
     CDS             2107..2919
                     /gene="nudC"
                     /locus_tag="VFV55_06750"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX4944036.1"
                     /translation="MIVTPADFTPLIAPAEHPSPMTFLFRGDEFLARNGDLALPDAAD
                     CAALGLAPGDMHPIGMLGTTYCRAAWLATETAAPEGFAFTGLRALFGNVDDSIVAVAG
                     RARQIVEWARTHRFCGACGSPAALVAGERAMKCTACGHSAYPRISPAMMVLVKRGPEI
                     LLARHRRGPGRYTALAGFLEPGESVEDAVHREVREEVGLEVKDLRYFASQSWPFPHSL
                     MIAFTAEYAGGDVRTEVAEIAEARWFGPGDELPEISIRQSVARALIDANLPR"
     gene            complement(2894..>3229)
                     /locus_tag="VFV55_06755"
     CDS             complement(2894..>3229)
                     /locus_tag="VFV55_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX4944037.1"
                     /translation="KAFSRYLGDDSATWESYDATALARSSAFAGPVLIDQGESDKFLA
                     EQLQPERFVAACKGTTIAPQLRRHAGYDHSYWFIASFIADHLRYHARALDARGPLADD
                     SLNGAGSRR"
BASE COUNT          467 a         1141 c         1125 g          496 t
ORIGIN      
        1 accgaggaat gctcgttctc gaccggggcg gtgttcgacc tctccggcgg ccgtgccgtc
       61 tactagcgcg atgggcgagc gatacgccgc ggccgcgctg aagggctttg cccgcgcgct
      121 tctcgagaag gcgggcatgg agccgccgaa ggccggcgac gtcgccctga ttctcgtcga
      181 gggcgacctg ctcggccacg acacccacgg cctcgccctc ctcgcgccgt acctccggga
      241 actcgaggcc ggcaccatgg cgaagtcggg ccagcccgag gtcctcaagc gccgcggcgc
      301 caccgaaacc tgggacggca agcgcctgcc cggaccgtgg ctcacgcttc gcgcgctcga
      361 tcgcgccatg gcgatggcgc gcgagttcgg caccgggacg gtggtgatcc ggcgcagcca
      421 ccacatcgcc tgcctggccg cctacctgcg ccgcctcacc gagcagggcc tggtggtgca
      481 gatcctttgc tcggacgcca attccgccag cgtcgcgcct ttcggtggca cgcaagcggt
      541 cttcaccccc aacccgatcg cgatcggctt ccccaccgtc ggcgacccgg tgctcgtcga
      601 catttcggcc tcgctggcga ccaatgccct caccaatcgc ctgcgcgccc agggagcgaa
      661 actgccccac gcgtggctcc tcgacgggca gggccagcct tccgacgatc cggcgacgct
      721 cgcggcggaa ccgaaaggca cgatcctgcc actgggcggc ctcgaggcgg ggcacaaggg
      781 atacggcctt gcgctcctcg tcgaggcgat gaccggaggc ctggcgggct tcggccgcgc
      841 cgatgcgaaa cagggctggg gcgccacggt gttcgtgcag gtgctcgacc ccgaggcatt
      901 cgccggcctc gcggatttca agcggcagac gcagtgggtg gcggacgcct gccgtgcgaa
      961 tgcgccgcgc ccgggattgc cgccggtgcg gctgccgggc gatggtggcc tggcaagacg
     1021 gcaggaacag ctcgcgggcg gcatcgccct gcacccggat atcctgcccg cgatcgcacc
     1081 gtgggcggag aagcttggcg tgccgctgcc cgccgctgcc tgaatcgcct cagcgcttca
     1141 cgatctccag gccatagatg cgctcctgcg cctcgatcat cgaggcgacc agcttgccgc
     1201 ccagccccat tgcaacagcc tgcgagaacg actggtgcac ggcttccccc acgaacgtcg
     1261 ccaccggcag cgattcggcg agccgcgtgt agtagcgcag gtccttggcc gcgttctcga
     1321 tcgagaactt catgcgcgtg aggtcgccct gcaccgcgcc gcgccccatc atctggaaga
     1381 tgttcgaatt gagcgcgccc tgcgaaacga cctccacgag catgtcgagc ggcacgccgg
     1441 ccttcgccgc caccgcgaac gcctcggcaa tggaagctgc aatggtgagg gccatgaagt
     1501 tgttcacgag cttcagcgtg tggcccgagc ccggcggacc cacgtggaag atgttctcgc
     1561 agaaggccgc gagcacgggg cggatcaagg cgaaggtggc gtcgtccgcg cccaccatcg
     1621 tgttgagccg gccctcctcg gcttccttcg gcgtgcgcgc gagcggcgca tcgacgaagc
     1681 gcacgccctt ggccgcgagc gcatcgcgca ggcgggcggt cgagccgggc tccgcggtcg
     1741 acgtgtcgat cacgatcatt ccgtcgcgcg cggcggaggc gatgccctgg gcgcccagca
     1801 cgttttcctc gacctgcggc gagccgctca cgcacaggat cacgacgtcc gaggtcgcgg
     1861 cgagttccgc gggcgtcttc gcctcgcggg cgccggcggc caggagatcg gcggcgcgtt
     1921 cgcggttgcg gtgcacgagg atcgcgaggg gaaagccctt cgcctgcaag ttcctcgcca
     1981 tcccgtggcc catcatgccc acgccgatga agccgatgcg cggtttcgcc tctgccatgg
     2041 atcgttctcc cgtggacgtg ctaggttcga ggtcgaggat aatacgcgct cgaccgcgcc
     2101 gcccccatga tcgtcacgcc tgccgatttc acgccgctca tcgctcccgc ggagcatccg
     2161 tcgccgatga cgttcctgtt tcgcggcgac gaattccttg cccgtaacgg cgacctggcg
     2221 cttcccgatg cggccgactg cgccgcgctg ggcctcgcgc ccggcgacat gcaccccatc
     2281 ggaatgctgg gaacgaccta ctgccgcgcc gcctggctgg ccacggaaac agcggccccc
     2341 gagggattcg ccttcacggg gctgcgcgcg ctattcggga acgtggacga ttcgatcgtg
     2401 gcggtggccg ggcgggcgcg ccagatcgtc gaatgggcgc gcacgcatcg cttctgcggc
     2461 gcctgcggaa gtcccgccgc gttggtggcg ggagagcgcg ccatgaaatg cacggcatgc
     2521 ggccattccg cctacccgcg catttcgcct gcgatgatgg tgctggtgaa gcgcggcccc
     2581 gagatcctgc tcgcccgcca tcgccgcggc cccgggcgct acacggcgct cgccggcttc
     2641 ctggagcccg gcgaatcggt ggaggacgcg gtgcaccgcg aggtgcgcga ggaagtcggg
     2701 ctggaggtga aggacctgcg ctacttcgcg agccagtcgt ggcccttccc gcactcgctc
     2761 atgatcgcct tcaccgcgga atacgcgggc ggcgacgtgc gcaccgaggt ggccgagatc
     2821 gccgaggcgc gctggttcgg gccgggcgac gagcttcccg agatctcgat ccgccagtcg
     2881 gtcgcgcgcg cgctcatcga cgcgaacctg ccccgttgag ggaatcgtcg gcgagcggcc
     2941 cgcgcgcgtc cagcgcccgg gcgtggtaac gcaggtggtc ggcaatgaag gacgcgatga
     3001 accagtagct gtggtcgtag cccgcgtggc ggcgcaactg cggcgcgatg gtggtgccct
     3061 tgcacgcggc cacgaagcgt tcgggctgca gctgctcggc gaggaacttg tcggattcgc
     3121 cctggtcgat gagcaccggc ccggcgaagg ccgacgagcg ggcaagcgcg gttgcgtcgt
     3181 agctttccca ggtcgcggag tcgtcgccga ggtagcggga gaatgcctt
//
LOCUS       DASUEV010000208         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_389903, whole genome shotgun sequence.
ACCESSION   DASUEV010000208 DASUEV010000000
VERSION     DASUEV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389903"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VFV55_06760"
     CDS             <1..1852
                     /locus_tag="VFV55_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX4944038.1"
                     /translation="GAVKQVLFEEDGAFRVGTILAEVGAAFQVEAAHGKRSKVKANAV
                     LLRFDGQALSSFIPEAQSRSEALDPQFLWEVCGREEFGFDELARDYYGHAPAPLEATA
                     VALALHASPMYFYRRGKGRYQAAPEENLRAALAGLERKRRQQEQVDAWAAAVGAGHLP
                     PELAAKLDSLLFKPDKMSLEWRALDQAATHAGLAPQKLLAKIGAISGPEDFFLRRFAF
                     ELFPGGLGFPEVPPVAAPQDLPDAAAPAFSIDDHETTEIDDAFSVERLPGGALRIGVH
                     IAAPSLFFARDHVLEALARERLSTVYFPGGKITMLPEAAIAEATLAEGREVPAASLYL
                     VADPDTLEVTRIESRLERVAIATNLRIVELETRLHEASLAEGVVEGAHGEDLLLLWRL
                     AKKLRAARGAGEEKSEKLDYTFRVEGGRVSIEPRRRGTPIDVLVSELMIHANATWGRM
                     LAEAGIPAIYRNQRGIKTRMEVEPGAHDWLGVTHYAWSSSPLRRFSDLANQRQLVALL
                     RGEPAPYALEELATAARDFETAYEAYAEHQRHLERYWSLRWIVQERLEGADATVIRDE
                     LVRLEGIPLVCRATGLPPSAPGDRVRVAFGEPDPWEVNLPCRYAGKPGGG"
     gene            1909..2823
                     /locus_tag="VFV55_06765"
     CDS             1909..2823
                     /locus_tag="VFV55_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4944039.1"
                     /translation="MHLRQTPPDALVHFSDYLLERFGLVASMQVAFVASVAFHAFAIV
                     GLGFRVPDPRGFDAPHNVLDVVLVNAKSASKPAKADALAQANLDGGGNTDKKRRASSP
                     FPVMDSSESAREVRQAQGRVQQLEREAAELMTRLKSKAAVAPPDPVADRDEKSDKTAR
                     DLIEKSLEIQRLEAQIRRDFQAYQERPRKKFVGARASEYRFAMYVDNWRLKIERVGNL
                     NYPDEARRQKLYGSLQLTVGLKPDGDVESVEINRSSGHRVLDQAAIRIVRLASPFDRF
                     PDVIRADTDILYITRTWTFTRADQISAE"
     gene            complement(2834..>4232)
                     /locus_tag="VFV55_06770"
     CDS             complement(2834..>4232)
                     /locus_tag="VFV55_06770"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX4944040.1"
                     /translation="RRDLRDEDNAGLHFALVSSRQVHCVFVFDREILDALPSRADRRV
                     EFIWESVLELRNALQGRGGGLIVLHGRAREEIPALAARLGAAAVFANEDYEPAALARD
                     TRVEKALAADGRRLHLAKDQVVFAKDEILTRSGGRYTVFTPYKRAWLAKLDDFYLKAY
                     PVAKHAGHLAKPAVETPLPPLASLGFEATDLARLAVPTGMSGARHLLSDFLPRMANYR
                     SARDFPAVRGVSGLSVHNRFGTISIRDLARESRQSKGDGAATWLSELAWRDFYFQILW
                     HHPHVATGAFRPGYDAIRWPGTDEHFAAWREARTGYPIVDAAMRQISRTGYMHNRLRM
                     IVASFLVKDLLVDWRRGEKHFADHLIDFDLAANNGGWQWAASTGCDSQPWYRIFNPVL
                     QSGKFDPKGEFIRRYLPELARVPDAFIHAPWTMAPREQPAYGCVIGRDYPAPIVDHAA
                     QRGRALALFKAARGR"
BASE COUNT          693 a         1442 c         1459 g          638 t
ORIGIN      
        1 tggcgccgtg aagcaggtgc tcttcgagga ggacggggcg tttcgcgtcg gcacgatact
       61 ggcggaggtc ggggcggcgt tccaggtgga agccgcgcac ggcaaacgct cgaaggtgaa
      121 ggccaacgcg gtgctcctgc gcttcgacgg gcaggcgctc tcctccttca tccccgaagc
      181 ccagtcgcgc tcggaagcgc tcgacccgca gttcctttgg gaagtctgcg gcagggagga
      241 attcggtttc gacgagctag cccgggacta ttacggccac gcgcccgcgc cgctggaggc
      301 gacggccgtg gcactcgccc tgcacgcgag cccgatgtat ttctaccgcc ggggcaaggg
      361 gcgataccag gccgcgcccg aggagaacct gagagccgcg ctggcgggcc tggaacggaa
      421 gcgccggcag caggagcagg tggatgcgtg ggccgcggcg gtcggcgcgg gccacctgcc
      481 cccggaactc gccgcgaagc tggactccct cctcttcaag ccggacaaga tgtcgctcga
      541 gtggcgggca ctggaccagg ccgccacgca cgccggtctc gcaccgcaga agctgctcgc
      601 gaagatcgga gcgatctcgg gccccgagga tttcttcctg cgccggttcg ccttcgagct
      661 ttttcccggg ggcctcggct tcccggaagt gccgccggtt gccgcgccgc aggacctccc
      721 ggacgccgcc gcgccggcct tttcgatcga cgaccacgag accacggaga tcgacgatgc
      781 cttctcggtg gagcgccttc ccggcggggc gctgcgcatc ggcgtgcaca tcgccgcgcc
      841 ctcgctcttc ttcgcgcgcg accatgtcct cgaggcgctg gcccgcgaac ggctctccac
      901 cgtctatttt ccgggcggga agatcacgat gcttcccgag gcggccatcg ccgaagccac
      961 gctggcggaa ggacgcgaag tgccggccgc ctcgctctac ctcgtggcgg atccggacac
     1021 gctcgaggtc acgcggatcg agtcgaggct ggagcgggtc gcgatcgcga ccaacctgcg
     1081 catcgtcgag ctggagacgc gcctccacga ggcctccctt gccgaaggcg tcgtggaagg
     1141 cgcccacggc gaggacctcc tgctgctgtg gcggctggcg aagaagctgc gcgctgcgcg
     1201 cggcgcgggc gaagagaaga gcgagaagct cgattacacg tttcgcgtgg aaggcggccg
     1261 cgtgagcatc gagccgcgcc ggcgcggcac gcccatcgac gtcctcgtct ccgagctcat
     1321 gatccatgcg aacgccacct ggggcaggat gctcgccgaa gccggtattc ccgcgatcta
     1381 ccgcaaccag aggggcatca agacgcgcat ggaagtcgaa ccgggcgcgc acgactggct
     1441 gggcgtgacc cattacgcgt ggtccagctc gccgctgcgg cgtttttccg atctcgccaa
     1501 ccagcgccag ctcgtggcgt tgctgcgcgg ggagccggct ccctatgccc tggaggagct
     1561 cgccacggcc gcgcgcgatt tcgagaccgc ctacgaagcg tatgcggaac accagcgcca
     1621 cctcgagcgc tactggagcc tgcgctggat cgtgcaggag cgactcgagg gagccgatgc
     1681 cacggtcatt cgcgacgaac tggtgcgcct ggaagggatt ccgctcgtct gccgcgcgac
     1741 cgggcttccg ccctcggctc cgggcgaccg ggttcgcgtg gctttcggcg agcccgatcc
     1801 gtgggaagtg aacctgccct gccgctatgc gggcaagccc ggcgggggat aggcgctgct
     1861 atcattcagc ttcatgttcc gcccgatcga gcgatcccgc gcctgccgat gcacctgagg
     1921 cagactcccc ccgacgcgct cgtccacttt tccgactacc tgctggagcg gttcgggctc
     1981 gtcgcctcga tgcaggtggc gttcgtggcc tccgtcgcct ttcacgcctt cgccatcgtg
     2041 gggctgggct tcagggtgcc cgatccccgg ggcttcgacg cgccgcacaa cgtgctggac
     2101 gtggtgctgg tgaacgcgaa gtcggcttcg aagcctgcca aggccgacgc gctggcgcag
     2161 gcgaacctgg acggcggagg aaacaccgac aagaagcgcc gggcttcgag cccgttcccg
     2221 gtcatggata gcagcgaaag cgcccgggaa gtccggcagg cgcagggacg cgtgcagcaa
     2281 cttgaacgcg aggccgccga actgatgacg cggcttaagt cgaaggccgc cgtcgcgccg
     2341 cccgatccgg tcgccgaccg cgacgagaag tccgacaaga cggcccggga cctcatcgag
     2401 aaaagcctcg agatccagcg gctggaagcc cagatccggc gcgacttcca ggcctaccag
     2461 gagcgcccgc gcaagaagtt cgtgggtgcg cgcgcgtccg agtaccgctt cgccatgtac
     2521 gtggacaact ggcggctcaa gatcgagcgc gtgggcaacc tcaactatcc cgacgaggcg
     2581 cgccggcaga agctctacgg gtcactgcaa ctcacggtgg gcctgaagcc cgacggcgac
     2641 gtggaatcgg tggagatcaa ccgttcctcg ggccacaggg tgctcgacca ggccgccatc
     2701 cgcatagtgc ggctcgcgtc tcccttcgac cgcttccccg acgtcatccg ggccgacacc
     2761 gacatcctct acatcacgcg gacgtggacc ttcacgcgcg ctgaccagat ttccgcggag
     2821 tgaccttgtc ccgctaccgg ccgcgcgcgg ccttgaagag ggccagagcc ctcccgcgct
     2881 gcgcggcgtg gtcgacgatg ggcgcaggat agtcgcgccc gatcacgcag ccgtacgccg
     2941 gctgctcgcg cggtgccatc gtccacgggg cgtgaatgaa ggcgtcgggg acgcgcgcaa
     3001 gctccggcag gtagcggcgg atgaactctc ccttcgggtc gaatttcccg gactgcaaca
     3061 cggggttgaa gatgcgatac cagggctgcg agtcgcaacc cgtcgaggcg gcccactgcc
     3121 agccgccatt gttggcggcg aggtcgaaat cgatgaggtg gtcggcgaag tgcttctcgc
     3181 cgcgccgcca gtcgacgagg aggtccttca cgaggaagga ggcgacgatc atccgcaggc
     3241 ggttgtgcat gtagcccgtc cggctgatct ggcgcatcgc cgcgtcgacg atcggatagc
     3301 ccgtgcgcgc ttcgcgccag gcagcgaaat gctcgtccgt gcccggccag cgaatggcgt
     3361 cgtacccggg ccggaaggcg cccgtggcca cgtgcgggtg gtgccacagg atctggaagt
     3421 agaaatcgcg ccatgccagt tccgacagcc acgtcgccgc cccgtccccc ttcgactgcc
     3481 gcgattcgcg cgcgaggtcg cggatggaaa tggtcccgaa gcggttgtgc accgaaaggc
     3541 cggagactcc ccgtacagcc ggaaagtcgc gggccgagcg atagttcgcc attcgcggga
     3601 ggaaatccga aagaaggtgc cgcgccccgg acatgcccgt cggcacggca agccgcgcga
     3661 gatcggtcgc ctcgaatccc agcgacgcga gcggcggcag cggcgtctcg acagcaggct
     3721 tggccaggtg tccggcatgc ttcgcgaccg gataggcctt gaggtagaaa tcgtccagct
     3781 tggcgagcca ggcgcgcttg taaggcgtga acacggtata tcggcccccg gaccgcgtca
     3841 ggatctcgtc cttcgcgaag accacctgat ccttggcgag atgcaggcgg cgcccgtccg
     3901 ccgcgagtgc cttttcaaca cgggtgtcgc gcgcgagggc ggccggctcg tagtcctcgt
     3961 tggcgaagac ggccgccgcg cccagccgcg ccgcgagcgc cgggatctcc tcgcgtgccc
     4021 ggccgtgcag gacgatgagg ccaccacctc tcccctgcag ggcgttgcgc aattccagga
     4081 cgctttccca aatgaactcg acgcgccggt cggcccggga aggcaacgca tccaggattt
     4141 cgcggtcgaa aacgaagacg cagtgaacct gccgggacga cacgagcgcg aaatgcagcc
     4201 cggcgttgtc ctcgtcgcgc aggtcgcgcc gg
//
LOCUS       DASUEV010000209        13755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_283680, whole genome shotgun sequence.
ACCESSION   DASUEV010000209 DASUEV010000000
VERSION     DASUEV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283680"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="VFV55_06775"
     CDS             <1..1901
                     /locus_tag="VFV55_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX4944041.1"
                     /translation="TAPLVVPMVAALFGAHWMLPVWLQWLLATPVQFVVGARFYRAAW
                     KALVARAGNMDLLVAIGTSAAYGLSVYLALAEKGAHLYFEASAVVITLVRTGKWLEER
                     AKRQTTAAIRALSALRPETARLLENGAERVVPVARVRVGDHVVVLPGERVPVDAEVTE
                     GSGDVDESLVTGESLPVAKSPGSRITGGSVNAEGRLVARTVAIGAESTLARIIRAVES
                     AQAAKAPIQRLVDRVSEVFVPVVVAIAFVTLLAWGFATGNWETAILNAVAVLVIACPC
                     ALGLATPTAVMVGTGVAARHGILIKDAEALEIAHRTRVVAFDKTGTLTEGRPTLVEVV
                     PLDIARDEALRLAAAVQRGSAHPLARAVLEVAERERVPIPPAQDLSTVAGRGVQARIK
                     ERTVAIASRRWAGELEVAGMDRLEKDAARLEAQGLTVSWLVESAPGKRALALLAFGDA
                     LKPQSAGAVARLLALGITPVLVTGDNRGAGEAAGRALGIDEVLSEVLPEEKAAVIARL
                     RASHGAVAMVGDGINDAPALAAADIGIAMGTGTDVAMHTAGITLMRGDPSLVADALDI
                     SRRTHAKIRQNLFWAFVYNLVGVPLAALGLLSPVIAGAAMALSSVSVVSNALLLARWK
                     AAGTRPRP"
     gene            1922..2371
                     /gene="cueR"
                     /locus_tag="VFV55_06780"
     CDS             1922..2371
                     /gene="cueR"
                     /locus_tag="VFV55_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HEX4944042.1"
                     /translation="MPQEKYFELSDARREGFLNISEAAAASGVSAKMIRHYEETGLVP
                     KAGRTVAGYRIYRDADVHVLRFIRRSRDLGFAMKEIAGLLGLWQDRRRASADVKRLAT
                     KHMADLDARIAELQGMRRTLEHLVHCCHGDGRPDCPILDDLAGKRHA"
     gene            2413..3048
                     /locus_tag="VFV55_06785"
     CDS             2413..3048
                     /locus_tag="VFV55_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4944043.1"
                     /translation="MALFADPDRSRALAAYGALADGYEASSRRILGVREAAVAALDLK
                     PGETVFDVACGTGATIPLLCRALGPEGRVLGIEQSPPMAAIARKRLPGCGCRGTLLEA
                     SVQDFATDLRADAMLFCYTHDVLQSPEALENLVRHAKPGCRVAVAGVRFLPWSWGFAV
                     NAFTAWRTRHYLTTYRGLRAPWEGLAGRCATFRVVRSFHWGSSYLGVGRLK"
     gene            3053..4057
                     /gene="dmeF"
                     /locus_tag="VFV55_06790"
     CDS             3053..4057
                     /gene="dmeF"
                     /locus_tag="VFV55_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEX4944044.1"
                     /translation="MALHDLTSWRHEHAFDAGNRAGESRTWIVVAITAVMMVAEIVGG
                     WLTGSMALLADGWHMGTHVAALSMAGIAYALARRWSGDERFAFGTWKIEVLGAFSSAL
                     VLAIVALAMAVESVLRLVSPHAIAFGQALAVAVIGLVVNVVSAFVLAGHDHGHDHDHG
                     GDHDHGQADEHDHAHDDGPGHETRGDHGHGDLNLRAAYFHVIADAFTSVLAIVALAAG
                     MMAGWAWLDPVMGIVGAGVIAWWSKGLLAQSARVLLDREMDTPVAAQVRMAIESDGDA
                     EIADLHVWRVGRARYACIVAVVAREPLTPDGYRERLADIASLAHVSVEVNRCPHENCP
                     "
     gene            4054..5760
                     /locus_tag="VFV55_06795"
     CDS             4054..5760
                     /locus_tag="VFV55_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944045.1"
                     /translation="MTDNAAKIVREFRHILLWPLQLRRLRRETGFANHWEVLRANPGP
                     WQEVKDNLLVDDESCQTGYQEFVYFLPYVQRFLYGFGEADAQTPSSLHMFRRDDIATA
                     RVRLAAADAPVELAVARTRLVFFYDQDVAILALEVEARDLPLQYAIDVMDRFGRPYPP
                     YWDTPGQGAHCTFQVEFLAADGTLLSASDYGDRDKYVGLVRDIKQTPLSLHWEYLLHP
                     LVPAYLGGGILQYYQIENKRIPIMSYLALDDPRVLTRGDLTRIGYAAKWGPSDTLPFA
                     PRFLADFEDRHCYDRYWDGTNPASGMNTRFVFCGVAFAMVTRHEDARTDLVHTFRRQF
                     FQIGLIANFHKAALLNLSNRFSIAVERLRVGDYASVRVFKESVREALELFLRFNHRYW
                     FHEISNQVLAADIFSRWAHQLGSDALFAEVREEGHDINRYLDAERTRKAGDNAMRLTV
                     VSALGMVGTVVTGFLGMNLFNHTELPGWDKFWIFMAVFVPTFALGLYTVVMSKRIATF
                     FEALSSERLTWRERFVAFGQIWRPPPRRPRVMMRVAGGDEPTRRSTGHPAKSRASAAT
                     RG"
     gene            5771..6355
                     /locus_tag="VFV55_06800"
     CDS             5771..6355
                     /locus_tag="VFV55_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX4944046.1"
                     /translation="MATLAEARDVLAFWFGEPGSTSHGKPRAAWFGKDADFDGEIRRR
                     FLALHACAALGERQHWRDDPETLLALVIVLDQFSRNLYRDDPRAFSQDAMALDGAQLM
                     VARGWDAARLPVERQFAYLPFEHAEDPAMQDRSVALFSTLEAFPETKGLAGWAEKHRV
                     IVRRFGRFPHRNAVLGRESTAEEIAFLKEPGSRF"
     gene            complement(6386..7009)
                     /locus_tag="VFV55_06805"
     CDS             complement(6386..7009)
                     /locus_tag="VFV55_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4944047.1"
                     /translation="MTTGPNSSQPPATLTTSRLRLRPWRESDLEPFAAMNADPRVMEH
                     FPSVQTREESEAVARRIMAKLESQGWGLWALEVVGGEPFIGFTGLAVPTFESWFTPCT
                     EIGWRLARGAWGQGYATEAARAALAFGFGELGLAEIVSFTAVSNRRSAAVMERLGMQR
                     AGEFDHPRIAEGNPVRRHVLYRIKRGQKKGGRLLFSPPSCGGKKTEK"
     gene            complement(7006..9711)
                     /locus_tag="VFV55_06810"
     CDS             complement(7006..9711)
                     /locus_tag="VFV55_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944048.1"
                     /translation="MYRQGVGDFKYFVGISSLAQIATREDRVCVLNILGGESSEVTPV
                     GHEYSGGNVVFGTSPGRRGQFLETRIGNVPVYNNVREGLSDGHRFNCGVIYLPPSAAR
                     DGVAELIRVNPDVKKIFMVTEKMSVHDAREIRAMGQANGIDIFGANCLGVADAWNQVR
                     IGGALGGDTPAEALRRGSIAIFSNSGNFTTTIATYLRMAGWGTTTLISSGKDVYIHYA
                     APEFAFALANDARTKAAVLYVEPGGYYELDATFTKPIVACVVGRWKAKLTRAVGHAGA
                     MAGGTDDAPGKERWFMDMFGVDGIFTPGRPIFSARGALVTNIAHIPLALAAVMRENAT
                     RPDFAPEGNLALKPWFGLNQGLDLPAALDLPVVEAAPPYNEQIAALARQIGCLFPRQP
                     MKDCSGASQMDAQSQVTSLYGVSMLDAAQHPLESNVGLALLHELGGENDRKLVNIAIG
                     AELNLHGQPELAAAEAARAAGNAPNSVLAAAASIVGPRRAQRAREAVVALIDLFSQAG
                     VKSALDETFDVGSIVADAATRELFVSGARDGKAEAMLAGLKARGARSVFVRYLESLPG
                     HPCADAVLAAITTTLAWGPLMRKRVSRLTAESLPWWMRLFGALVGASVDAQRHEAGRF
                     CGIATAEILGQRSLTELAYIALLGLHPQPANLFAFQTLVGLLLTNGPGAISAQGAKGA
                     VSADGPESPERVQLNKGLVGFLTHSGYAHGGNGYEGIAFLLAQFRDSGLEDPTDPGHG
                     IDLAALAMRYVDEYARYKSRKKTAGSLDIQKIPGVNHPVFKDRPVNFDPREVYVRELF
                     AKRGEYNVFHEYYHALVRALFDAGVSRTVYCVNIDAVIAALLLKMLWEPYRSGAFPGQ
                     ALETAAFTIFLYPRMLGCAAEIDDHLNRGRNLDTRTPASQVRFVA"
     gene            complement(9759..11048)
                     /locus_tag="VFV55_06815"
     CDS             complement(9759..11048)
                     /locus_tag="VFV55_06815"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HEX4944049.1"
                     /translation="MQVTGMLYGSKLLQFVGFPTSEVLGPSASEEEIKALIDRHGSVF
                     VKPVFKGGVGKKGKAGLLGRASDLKTALKEKERLYFVEHRHGNQSAKANGVTFEGAVP
                     AEHEVYFSIADSTHFRAPTMTLTHHGGMDIEELDKSLVANVPFDPLTGLKAFVVANAL
                     SELNAPKELISPLVQQLPKLWELFHDFGMTTLELNPIRMRPDHQGRLTPVACDFKCGF
                     DRDDPRWQRLHLPAHVFAIDYSDFEQEINQLRTYQGQSDVYVINSGGTILAPTFGGGA
                     NSLVTEMLGDDAIISSDFGGNPPYEKMKEVARICFKHWLKQSNVLFIIGGKSNNTDIF
                     ETFRAMADALREHFSRHGPTPLFVVIGRGGPNLVRGMGAMRDTIEALGLPYRMFGFDS
                     DMSEVVEYARQVNDWMKAGGRAQLAARLKVAQPATAD"
     gene            complement(11336..12517)
                     /locus_tag="VFV55_06820"
     CDS             complement(11336..12517)
                     /locus_tag="VFV55_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944050.1"
                     /translation="MKTALIAVACALAAPAFAQSPDAQHERHVIIDGHDFLADAASAR
                     TTADAWRQWGDDLRASLGTMFGDHFAAARTVKGAPYSADIATEANQTLADGNVIARKT
                     TGRVYRDSEGRTRQETVVNGEVKSIQIGDPVEGTAVMLLPGSKRAVRMPHLSLHKDAK
                     ERKVLRLADKEIRIEDGKVTVDGKEARGKVEITAGGKEIRIENGKVTIDGREFTPGEG
                     GRKVIVKHLEEGETGDGTRREEVRVHVLRSGDGKEAHAFMPPMPPVPPTPPMPPMPPM
                     SAMDALGTLGARLDGALLRSKGTTTSLGVKEFEGVKAEGKSTVRTIPAGDIGNKAPIM
                     VTSESWYSPELQVTVYSRQIDPRYGETIYRLTNIRRAEPSADLFKVPEEYAVRGKRDR
                     G"
     gene            complement(12542..13051)
                     /locus_tag="VFV55_06825"
     CDS             complement(12542..13051)
                     /locus_tag="VFV55_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944051.1"
                     /translation="MNTPDDTLHPAVREARLTERFDALRSELARVRAPAHLEASLVEE
                     FRSRRAGNIGAARPRLWWMPPLAMAATVAAVSWMVRAPGDLPVPDPAIEFTIEPAARG
                     DAGPFLALTPLERIALEPQATVVTTEFPRALLAQWGLPVSPERAGEPVRAEMLYSAEG
                     EALAVRVLN"
     gene            complement(13048..13653)
                     /locus_tag="VFV55_06830"
     CDS             complement(13048..13653)
                     /locus_tag="VFV55_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4944052.1"
                     /translation="MPPRNMQPELTLFARMRQGDEEAFVALFRRHKDAVYRFALLRTG
                     SAALAADVTQETFLHLMTRPEQYDPSRGCVAAWLCGVARNFARRESGGREDATDPDDL
                     ADDTMHAPELVDPVTPADQLLAGETAGQVRAALAAVAPHYRDVLILCELSELSYAEVA
                     QVCGIDIGTVRSRLSRARAQLADRLSRMGLMSGPGRVKEAS"
BASE COUNT         2053 a         4707 c         4723 g         2272 t
ORIGIN      
        1 tcacggcgcc gctcgtcgtg cccatggtcg cggccctttt cggcgcgcac tggatgctgc
       61 ctgtgtggct gcagtggctc ctcgccaccc ccgtgcagtt cgtggtcggg gcgcggttct
      121 atcgcgccgc ctggaaggcg ctcgtcgcgc gcgccggaaa catggatctc ctcgtcgcga
      181 tcggcaccag cgccgcctac ggactgagcg tgtatctcgc gctcgcggaa aagggcgcgc
      241 acctctattt cgaggcttcc gcggtggtca tcactctggt gcgcacgggc aagtggctcg
      301 aggagcgcgc caagcgccag accacggcgg cgatccgggc cttgtcggcg ctgcggcccg
      361 aaacggcgcg actcctcgaa aatggagcgg agcgcgtcgt gccggtggcg cgggtgcggg
      421 tgggggacca cgtggtcgtc ctgcccggcg agcgcgtgcc cgtcgatgcg gaagtgaccg
      481 aaggaagcgg cgacgtggac gaatccctcg tcaccggcga gagcctgccg gtggccaagt
      541 cgcccggcag ccgcatcacc ggagggtcgg tgaatgccga aggccgcctc gtcgcgcgca
      601 ccgttgccat cggcgccgag agcacgctcg cccggatcat ccgcgcggtg gaatcggcgc
      661 aggctgcgaa ggcccccatc cagcgcctcg tcgatcgcgt gagcgaagta ttcgtgccgg
      721 tggtggtggc gatcgccttc gtcacgctgc tggcctgggg cttcgcaacc ggcaactggg
      781 aaaccgcgat cctcaacgcc gtggccgtcc tcgtcatcgc ctgcccgtgc gccctggggc
      841 tcgccacgcc cacggcggtc atggtcggga ccggcgtggc cgcgcgccac ggcatcctga
      901 tcaaggacgc cgaagcactc gagatcgccc accgcacgcg cgtcgtggcg ttcgacaaga
      961 cgggcacgct caccgagggc aggcccaccc tcgtcgaggt ggtaccgctg gacatcgcgc
     1021 gcgacgaggc gctgcgcctc gcggctgcgg tgcaacgcgg ctccgcgcac ccgctcgccc
     1081 gggccgtgct cgaggtcgcc gagcgcgagc gcgttccgat tcccccggcg caggacctga
     1141 gcacggtcgc cggccgcgga gttcaggcac gcatcaagga gcgcacggtc gcgatcgcga
     1201 gccggcgctg ggccggggag ctggaggtcg cgggcatgga ccggctggaa aaggatgcgg
     1261 cgcggctcga ggcgcaggga ctcaccgtgt cgtggctcgt cgaatcggcg cccgggaaac
     1321 gcgcgctggc gcttctcgcc ttcggggacg cgctcaagcc gcagtcggcc ggcgccgtcg
     1381 ctcgcctgct cgcgctcggc atcacgcccg tccttgtcac gggcgacaat cgcggcgcgg
     1441 gcgaggcggc gggccgcgcg ctgggcatcg acgaggtgct ctccgaggtc ctgcccgagg
     1501 aaaaggctgc ggtcattgcg cggctgcgcg cctcgcacgg ggcagtggcg atggtcggcg
     1561 acggcatcaa cgacgcgccc gcgctcgccg cggccgacat cggcatcgcg atgggcaccg
     1621 ggaccgacgt ggccatgcac accgcgggaa tcacgctgat gcgcggcgac ccgtcgctgg
     1681 tggccgacgc gctcgacatc tcgcgtcgca cccatgcgaa gatccgccag aacctcttct
     1741 gggctttcgt gtacaacctg gtgggcgtgc cgctcgcggc cctgggcctg ctctccccgg
     1801 tgatcgccgg cgctgcgatg gcgctcagca gcgtgagcgt ggtgtcgaac gcgttgctgc
     1861 ttgcgcggtg gaaggccgcg ggcacccggc cgcgaccctg agcaaccgga ggcgatcgac
     1921 catgccgcag gaaaagtact tcgagctttc cgacgcgcga cgcgagggat tcctcaacat
     1981 cagcgaggcg gccgcggcct ccggggtgtc ggccaagatg atccgtcact acgaggagac
     2041 cgggctggtg ccgaaggcgg gtcgcacggt cgccggctac cgcatctacc gcgacgccga
     2101 cgtgcatgtg ctgcgcttca tccgccgctc gcgcgacctg ggcttcgcga tgaaggagat
     2161 cgccggcctg ctcggcctgt ggcaggaccg gcgccgcgcc agcgccgacg tgaagcggct
     2221 ggcgacgaag cacatggccg acctcgacgc gcgcatcgcc gaattgcagg gcatgcgccg
     2281 cacgctggag cacctcgtgc attgctgcca cggagacggc cggcccgact gcccgatact
     2341 cgacgacctt gccgggaagc ggcacgcgta ggcttagaat acgggccgct accacttccg
     2401 ggagttgaat cgatggccct gttcgcggat cccgaccgga gccgcgcgct cgcggcctac
     2461 ggtgccctcg ccgacggtta cgaggcctcc agccggcgca tcctcggcgt ccgcgaagcg
     2521 gcggtcgcgg cgctcgacct gaagcccggc gagaccgtct tcgacgtggc ctgcggcacc
     2581 ggggcgacga ttccgctcct gtgccgtgcc ctgggcccgg aggggcgagt gctcggcatc
     2641 gagcaaagcc cgccgatggc cgccatcgcg cgcaagcgac ttcccggctg cgggtgccgt
     2701 ggcacgctgc tcgaggccag cgtgcaggac ttcgcgaccg acctgcgcgc cgacgcgatg
     2761 ctcttctgct acacacacga cgtcctgcag tcgcccgagg cgctcgagaa cctcgtgcgg
     2821 cacgcgaagc ccggctgccg cgttgcggtg gcgggtgtgc gcttccttcc gtggagctgg
     2881 gggttcgccg tcaatgcctt caccgcgtgg cgcacgcgcc actacctcac tacctaccgc
     2941 ggccttcgcg cgccctggga gggcctcgcc gggcgctgcg caactttccg tgtcgtgcgc
     3001 tcttttcact ggggatccag ctatctcggc gtcggtcgcc taaaatagcc tgatggcact
     3061 ccacgacctc acttcctggc ggcacgagca cgcattcgac gcgggcaacc gggcgggaga
     3121 atcgcgcacc tggatcgtcg tggccatcac ggccgtcatg atggtggccg agatcgtcgg
     3181 cggttggctt acgggatcca tggcgctcct ggccgacggc tggcatatgg gcacgcacgt
     3241 ggcggccctt tcgatggccg gcatcgccta cgcgctcgcg cggcgctggt cgggcgacga
     3301 gcgctttgcc ttcgggacgt ggaagatcga ggtgctcggc gcgttttcga gcgccctggt
     3361 gctcgccatc gtcgccctgg ccatggcggt ggaatcggta ctgcgcctgg tcagcccgca
     3421 cgcgatcgcc ttcggccagg cgctggccgt cgcggtcatc ggcctcgtgg tcaacgtggt
     3481 gtcggcattc gtcctcgccg gacacgacca cgggcacgac catgatcacg ggggcgatca
     3541 tgaccacggt caggcggacg aacatgacca tgcccacgat gacggtcccg gccacgagac
     3601 tcgcggcgac cacgggcacg gcgacctgaa cctgcgcgcc gcctacttcc atgtcatcgc
     3661 ggatgcgttc acctcggtgc tcgccatcgt ggcgctggcc gcggggatga tggccggctg
     3721 ggcgtggctg gacccggtga tgggcatcgt cggcgcgggc gtcatcgcgt ggtggtcgaa
     3781 gggcctcctc gcccagtcgg cgcgcgtgct gctcgaccgc gagatggata cgcccgtggc
     3841 cgcgcaggtg cgcatggcga tcgagagcga cggcgatgcc gagatcgcgg acctgcacgt
     3901 ctggcgagtc gggcgcgccc gctacgcgtg catcgtggcc gtggtcgcac gcgaaccgct
     3961 gacgcccgac ggataccgcg agcggctggc ggacatcgcc tcgctggcgc acgtttccgt
     4021 cgaggtcaat cgctgcccgc acgagaactg cccgtgaccg acaacgccgc caagatcgtc
     4081 cgcgagttcc gccacatcct cctctggccg cttcagcttc gccggctgcg gcgcgagacc
     4141 ggtttcgcca accactggga agtgctgcgc gccaatcccg gcccgtggca ggaagtgaag
     4201 gacaacctgc tggtcgacga cgagtcctgc cagacgggct accaggagtt cgtctacttc
     4261 ctgccctacg tgcagcgttt cctctacggc ttcggcgagg ccgatgccca gacgccttcg
     4321 agcctgcaca tgttccggcg cgacgacatc gccaccgccc gggtgcggct ggccgcggcc
     4381 gacgcgcctg tcgagctcgc ggtcgcgcgc acgcggctcg tgttcttcta cgaccaggac
     4441 gtcgccatcc tcgccctcga ggtcgaggcg cgcgaccttc cgctccagta cgcgatcgac
     4501 gtgatggacc gcttcggccg accgtatccc ccgtactggg acacgccggg gcagggagcg
     4561 cactgcacct tccaggtgga gttcctcgcc gcggacggga cgcttctctc cgcttcggac
     4621 tacggcgacc gcgacaagta cgtcggcctg gtgcgcgaca tcaagcagac gccgctgtcg
     4681 ctgcactggg aatacctgct gcatccgctg gtgcccgcct atctcggcgg cggcatcctg
     4741 cagtactacc agatcgagaa caagcgcatc ccgatcatga gctatctcgc gctggacgat
     4801 ccgcgcgtac tcacgcgcgg ggatctcacg cgcatcggat atgccgcgaa atggggcccg
     4861 tcggacacgc tcccgttcgc gccgcgcttc ctcgccgatt tcgaggacag gcactgctac
     4921 gaccgctact gggacggcac caacccggcg agcgggatga acacgcgctt cgtcttctgc
     4981 ggagtggcct tcgccatggt cacccggcac gaggatgcgc gcacggacct ggtgcacacc
     5041 ttccgccggc agttcttcca gatcggcctc atcgccaact tccacaaggc ggccctgctc
     5101 aacctttcca accggttctc gatcgccgtc gaacgcctga gggttggcga ctatgcgagc
     5161 gtcagggtat tcaaggagag cgtgcgcgag gcgctcgagc tgttcctgcg cttcaaccac
     5221 cgctactggt tccacgagat ctccaaccag gtgctggcgg ccgacatctt ctcgcgctgg
     5281 gcccaccagc tgggcagcga cgcgctcttc gcggaggtgc gcgaggaagg gcacgacatc
     5341 aatcgctacc tcgacgcgga acgcacgcgg aaggcgggcg acaacgcgat gcgcctgacg
     5401 gtggtctccg ccctcgggat ggtgggcacc gtggtgaccg gcttcctcgg catgaatctc
     5461 ttcaaccata cggaattgcc gggctgggac aagttctgga tcttcatggc ggtgttcgtg
     5521 cccacctttg ccctgggcct ctacacggtg gtgatgtcga agcgcatcgc gaccttcttc
     5581 gaggcgctgt cctcggagcg cctcacctgg cgcgaaaggt tcgtggcatt cgggcagatc
     5641 tggcggccgc cgccgcgccg cccccgggtg atgatgcgcg tggccggcgg ggacgaaccc
     5701 acgcggcgaa gcacggggca cccggcgaag agccgggcat cggccgcgac gcgcggatga
     5761 cgggcgcggc atggccaccc tcgcagaggc gcgcgacgtc ctcgcattct ggtttggcga
     5821 gccgggatcg accagccacg gcaagcctcg cgcggcttgg ttcggcaagg acgccgactt
     5881 cgacggggag atccggcgcc gcttcctcgc gctgcacgcg tgcgcggcgc tgggcgagcg
     5941 ccagcactgg cgcgacgatc ccgaaaccct cctcgcgctg gtgatcgttc tggaccagtt
     6001 ctcgcgcaac ctctatcgcg acgatccgcg cgcgttctcg caggacgcga tggcgctcga
     6061 tggcgcgcag ctcatggtcg cgcgcggctg ggatgcggcg cggctgccgg tggaacggca
     6121 gttcgcctac ctgccgttcg agcacgccga ggatcccgcg atgcaggacc gctcggtcgc
     6181 gctcttctcc acgctcgagg ccttccccga gacgaagggt ctcgccgggt gggccgaaaa
     6241 gcaccgcgtg atcgtccggc gcttcgggcg cttcccgcac cgcaatgccg tgctgggccg
     6301 cgaatcgacg gcggaggaga ttgccttcct gaaggagccc ggctcccgct tctgatcaaa
     6361 agaggtcaga agaaaagggg tcaggttact tttctgtctt cttgccgccg catgacggcg
     6421 ggctgaaaag taacctgccc ccttttttct gacccctttt gattctgtag agcacatgcc
     6481 gccgcacggg gttgccctcc gcgatccgcg ggtggtcgaa ctccccggcg cgttgcatgc
     6541 cgaggcgttc catcacggcc gccgatcgcc tgttggagac cgcggtgaag gaaacgatct
     6601 cggccagccc gagttcaccg aatccgaagg cgagagccgc ccgcgcggcc tccgtagcat
     6661 agccctggcc ccaggcgccg cgggccaggc gccagccgat ctccgtgcac ggcgtgaacc
     6721 aggactcgaa ggtcggcacg gcgagccccg tgaagccgat gaacggctcg cccccgacca
     6781 cctcgagtgc ccagagcccc cagccctgcg actccagctt cgccatgatg cgacgggcca
     6841 cggcttcgct ttcctcgcgc gtctggaccg aagggaagtg ttccatcacg cgcggatccg
     6901 cgttcattgc cgcgaagggc tcgaggtcgg attcgcgcca ggggcgcagg cgcagccggg
     6961 acgtggtgag cgtcgcgggc ggctgcgagg aattcgggcc ggtcgtcatg ccacgaaccg
     7021 gacctgcgac gccggtgtgc gcgtgtccag gtttcggccg cgattcaggt gatcgtcgat
     7081 ctcggcggcg cagcccagca tgcgcggata gagaaagatc gtgaacgccg cggtctccag
     7141 ggcctgcccg gggaaagcgc cgctgcggta cggctcccac aacatcttga ggagcagcgc
     7201 cgcgatgacg gcatcgatgt tgacgcagta gacggtgcgc gacacgccgg cgtcgaacaa
     7261 ggccctgacc agggcgtggt aatactcgtg gaacacgttg tattcgccgc gcttcgcgaa
     7321 cagttcgcgc acgtacactt cgcgcggatc gaagttgacg ggccggtcct tgaacaccgg
     7381 gtggttgacg ccggggatct tctggatgtc gaggctgccg gccgtcttct tcctcgactt
     7441 gtagcgtgcg tattcgtcca cgtagcgcat cgccagcgcg gccaggtcga tcccgtggcc
     7501 gggatccgtc ggatcctcga gcccggaatc gcggaactgc gccagcagga acgcaatccc
     7561 ctcgtacccg ttgccgccat gggcgtaccc cgaatgcgtg aggaagccga ccaggccctt
     7621 gttgagctgc acgcgttcgg gggactccgg accgtcggcg gacaccgcac ccttggcgcc
     7681 ctgcgcggaa atcgcgccgg gaccattggt caggagcagt cccaccaatg tctggaaggc
     7741 gaacaggttc gccggctggg ggtgaagtcc gagcagggcg atgtaggcca gttccgtcag
     7801 cgagcgctgc ccgagtatct cggcagtggc gatgccgcag aacctgcccg cttcatggcg
     7861 ctgcgcatcg acggacgcgc cgaccagcgc gccgaacagc cgcatccacc agggcaggct
     7921 ctccgccgtc agccgggaca cgcgcttgcg catcagcgga ccccaggcca gcgtcgtcgt
     7981 gatcgcggcc agcaccgcat cggcgcaagg gtgcccgggg aggctttcca ggtagcgcac
     8041 gaacaccgat ctggccccgc gcgccttcag gccggccagc attgcctccg cctttccatc
     8101 gcgcgcgccg gatacgaaca gctcgcgcgt ggcggcatcg gcaacgatcg aaccgacgtc
     8161 gaaggtctcg tcgagggcgc tcttcacgcc tgcctgcgag aagagatcga tcagcgcgac
     8221 aaccgcttcg cgcgcgcgct gcgcgcggcg cggaccgacg atgctcgccg cagcggcaag
     8281 cacgctgttg ggtgcgttgc ccgccgcacg cgccgcctcc gccgccgcca gctcgggctg
     8341 gccgtgcagg ttgagttccg cgccgatcgc gatgttgacc agtttccggt cgttctcgcc
     8401 gcccagctcg tgcagcagcg cgaggccgac gttggattcc agcggatgtt gcgcggcgtc
     8461 cagcatcgag acgccgtaca ggctggttac ctgcgactgg gcgtccatct gggatgcgcc
     8521 cgagcagtcc ttcatcggct gccgcgggaa caggcagccg atctgccttg ccagcgcggc
     8581 gatctgctcg ttgtacggcg gtgccgcctc caccaccggc aggtccagcg ccgccggcag
     8641 atcgaggccc tggttcaagc cgaaccacgg cttgagggcg aggttcccct cgggcgcgaa
     8701 atcgggccgc gtcgcgtttt cgcgcatcac ggccgccagc gccaggggga tgtgcgcgat
     8761 gttggtgacc agcgcgcccc gggccgagaa gatcggcctg cccggcgtga agatcccgtc
     8821 cacgccgaac atgtccatga accagcgctc cttgccgggg gcatcgtcgg tgccgcccgc
     8881 catcgcgccg gcgtgcccga ccgcgcgcgt gagcttggcc ttccagcgtc ccaccacgca
     8941 ggcgacgatc ggcttggtga aggtcgcgtc cagttcgtag tagccgcccg gctcgacgta
     9001 caggactgcc gccttggtcc gcgcatcgtt ggccagcgcg aacgcgaatt cgggcgcggc
     9061 gtaatggatg tacacgtcct tgccgctcga aatgagggtg gtcgtccccc atccggccat
     9121 gcgcaggtag gtggcgatcg tcgtggtgaa gttgccggaa ttggagaaga tggcgatcga
     9181 tccgcgtcgc agcgcctccg ccggcgtgtc gccccccagt gcgccgccga tgcgcacctg
     9241 gttccaggca tcggccacgc ccaggcagtt ggcgccgaag atgtcgatgc cgttggcctg
     9301 gcccatcgcc cggatctcgc gcgcatcgtg cacggacatc ttctccgtca ccatgaagat
     9361 cttcttcacg tccgggttca cccggatcaa ttcggccacg ccgtcgcgcg ctgccgaagg
     9421 cggcaggtag atcacgccgc aattgaagcg gtgcccgtcg gagaggccct cgcgcacgtt
     9481 gttgtagacc gggacgttgc cgatccgggt ctcgaggaac tggccgcggc ggccgggcga
     9541 ggtgccgaac accacgttgc cgccggagta ctcgtggccc acgggcgtca cttcgctcga
     9601 ctcgccgccg aggatgttga gcacgcagac gcggtcctcg cgcgtggcga tctgcgcgag
     9661 cgagctgatg cccacgaaat acttgaaatc cccgactccc tgcctgtaca tgacggtctc
     9721 ccggtattcc tggattgcgc ccggttggag gcgcccggtc agtccgccgt cgcgggctgg
     9781 gcgaccttga gacgcgccgc gagttgcgcg cgcccgccgg ccttcatcca gtcattgacc
     9841 tgccgcgcgt actcgaccac ctcgctcatg tcggaatcga agccgaacat gcggtagggc
     9901 aggcccagcg cctcgatggt gtcgcgcatc gcgcccatgc cgcgcacgag gttcgggccg
     9961 ccgcgtccga tcacgacgaa gagcggcgtc ggcccgtgcc ggctgaaatg ctcgcgcagc
    10021 gcgtcggcca tcgcgcggaa cgtttcgaag atgtcggtgt tgttggactt gccgccgatg
    10081 atgaacagca cgttggactg cttcagccag tgcttgaagc agatgcgcgc cacttccttc
    10141 atcttttcgt agggcggatt gccgccgaaa tcggaagaga tgatggcgtc gtcgccgagc
    10201 atctcggtca ccagcgaatt ggcgccgccg ccgaacgtcg gcgcgaggat ggtgccgccg
    10261 gagttgatca cgtagacgtc gctctggccc tggtaggtgc gcagctggtt gatctcctgc
    10321 tcgaagtcgg agtagtcgat ggcgaacacg tgcgcgggca ggtgcagccg ctgccagcgc
    10381 ggatcgtcgc ggtcgaagcc gcacttgaag tcgcaggcga cgggcgtcaa ccggccctgg
    10441 tggtccgggc gcatccggat cgggttgagc tcgagggtcg tcatgccgaa gtcgtgaaac
    10501 agctcccaca gcttgggcag ctgctgcacc agcggcgaga tgagttcctt cggcgcgttc
    10561 agctcggaca gggcattggc gacgacgaac gccttgaggc cggtgagcgg atcgaacggg
    10621 acattggcca ccagcgactt gtccagttcc tcgatgtcca tgccgccgtg gtgcgtcagc
    10681 gtcatggtgg gcgcgcggaa gtgggtcgag tcggcgatcg agaaatagac ctcgtgctcg
    10741 gccggcaccg cgccttcgaa ggtcacgccg ttggccttgg ccgactggtt tccgtgccgg
    10801 tgctcgacga agtagagccg ctccttctcc ttgagcgcgg tcttgaggtc gctcgcccgg
    10861 ccgaggaggc ccgccttgcc cttcttgccc acgccgccct tgaacacggg cttcacgaac
    10921 accgagccgt ggcggtcgat caatgccttg atctcttcct cgcttgccga gggcccgagc
    10981 acttcggagg tcgggaagcc gacgaactgc agcagcttcg acccgtagag catccctgtg
    11041 acttgcacgt cacccccctg ctgccgggta ttgcgcccgg tcgcctcacg gtcctgcgcc
    11101 gcttgccgtg gcgcgcccca gtcgccgagg gagaagcata acttcacgcg cctttcgctg
    11161 aaaaggggtc aggttacttt gcacaagaat taggcaagac ggaaaagggg tcaggctact
    11221 tttcagtatg ctgtcttgcg acggcaatac gacagaaaag taacctgacc ccttttcagc
    11281 gcagctcgcg ctggcgaggt ccacgacacc ctgcggccgc gacgggccgg gacggctaac
    11341 cccggtcgcg cttgccgcgc acggcgtatt cctcgggcac cttgaagaga tccgcggacg
    11401 gctcggcgcg ccggatattc gtgaggcgat agattgtctc tccgtaccgc gggtcgatct
    11461 ggcgcgagta caccgtcacc tgcagttcgg gcgagtacca cgactccgag gtgaccatga
    11521 tcggggcctt gttgccgata tcgcctgcgg ggatcgtgcg cacggtcgac ttgccctcgg
    11581 ccttcacgcc ctcgaattcc ttcacgccga gcgaagtcgt ggtgcccttc gagcgcagca
    11641 atgcgccgtc gagccgcgcg cccagggtgc ccagcgcgtc catggcggac atcggcggca
    11701 tgggtggcat cggcggcgtg ggaggcactg gcggcatcgg cggcatgaac gcgtgcgcct
    11761 ccttgccatc gccactgcgc agcacgtgca cgcgcacttc ctcgcgacgc gtgccgtcgc
    11821 cggtctcgcc ttcctcgagg tgcttcacga tcaccttccg gccgccttcg cccggtgtga
    11881 attccctgcc gtcgatcgtg accttgccgt tctcgatgcg gatctccttg ccgcccgcgg
    11941 tgatctccac cttgccgcgg gcctccttcc cgtccacggt gaccttgccg tcctcgatgc
    12001 ggatttcctt gtcggccagg cgcaggacct tgcgctcctt tgcgtccttg tgcagggaga
    12061 ggtgcggcat gcgcacggcc ctcttcgagc ccggcagcag catcaccgcc gtgccctcga
    12121 cgggatcgcc gatctggatc gacttgacct cgccgttcac gaccgtctcc tgccgcgtgc
    12181 ggccctcgct gtcgcgatag acgcggcccg tcgtcttcct ggcgatgaca ttgccgtcgg
    12241 cgagcgtctg gttcgcctcg gtggcgatgt cggcgctgta gggagcgccc ttcaccgtcc
    12301 tggcggccgc gaaatgatcc ccgaacatcg tgccgaggga agcgcgcaga tcgtcgcccc
    12361 attggcgcca cgcatcggcc gttgtccttg cggaggcggc gtcggcgagg aagtcatgac
    12421 cgtcgatgat gacgtgtcgc tcgtgttgcg catcgggcga ttgcgcgaat gcgggtgcgg
    12481 ccagggcgca ggcgacggcg atgagtgcgg ttttcatgga tttcctcccg ggtgagtccg
    12541 ttcagttgag aacgcgcacg gcgagagcct cgccctcggc cgaataaagc atctcggcac
    12601 gaaccggctc gccggcccgc tcgggggaga ccggcaggcc ccattgggcg agcagggcgc
    12661 gcgggaattc ggtggtgacc acggtggcct gcggttccaa tgcgatgcgt tccaagggcg
    12721 tgagcgcgag gaacggaccc gcatcccccc tggcggccgg ctcgatcgtg aattcgattg
    12781 ccggatcggg cacgggcaga tccccgggcg cacgcaccat ccagctcacg gcggccacgg
    12841 tggccgccat ggcgagcggc ggcatccacc acagtcgcgg gcgggcggcg ccaatgtttc
    12901 cggcgcggcg ggagcggaat tcctccacga ggctcgcctc gaggtgagcg ggggcccgca
    12961 cgcgggcgag ctcggacctg agcgcatcga atcgctcggt cagccgcgct tcgcgcacgg
    13021 cggggtgcaa ggtgtcatcg ggggtgttca tgaagcctcc ttgacgcggc cggggccact
    13081 catcagcccc atccgcgaga gccggtcggc aagctgggcg cgggcgcggg aaagccgcga
    13141 gcgcaccgtt ccgatgtcga tgccgcagac ctgcgcgacc tcggcgtacg agagttcgga
    13201 tagttcgcaa aggatgagga cgtcgcggta atgcggggcg acggcggcga gtgccgcgcg
    13261 cacctgcccg gcggtctcgc cggcgaggag ctggtccgcg ggcgtgacgg gatccacgag
    13321 ttccggtgcg tgcatggtgt cgtcggcaag atcgtcgggg tcggtcgcgt cctcgcgccc
    13381 gccgctttcc cgccgcgcga agttcctcgc cacgccgcac agccaggcgg cgacgcagcc
    13441 gcgcgacggg tcgtactgct cggggcgcgt catcaggtgc aggaacgtct cctgcgtcac
    13501 gtccgcggcg agggccgccg aacccgtgcg cagcagcgcg aatcggtaca ccgcgtcctt
    13561 gtgcctgcga aacagcgcga cgaaggcctc ctcgtcgcct tgcctcatgc gggcgaacag
    13621 ggtcagctcg ggctgcatgt tcctgggggg catggacggt ttcgcatcgc tcttttccgt
    13681 attgcagcga aaccgccgga aagttcccgg cgtggaaaag gggtccaacg gaaaagcgga
    13741 aaaggggtca ggtta
//
LOCUS       DASUEV010000210         5217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_69513, whole genome shotgun sequence.
ACCESSION   DASUEV010000210 DASUEV010000000
VERSION     DASUEV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69513"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFV55_06835"
     CDS             <1..438
                     /locus_tag="VFV55_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HEX4944053.1"
                     /translation="QWLRCGPPGAGHFVKMIHNGIEYGLMQAYAEGFALMQGHPDFDL
                     DLAAVARTWMQGSVVRSWLLELTRDFLAEDASLEGVAPVVADSGEGRWTAQEAIDRGV
                     PAPVITAALMQRFASQGGDDFAARVLATMRNRFGGHAVPGKDG"
     gene            480..1820
                     /gene="argG"
                     /locus_tag="VFV55_06840"
     CDS             480..1820
                     /gene="argG"
                     /locus_tag="VFV55_06840"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF003779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX4944054.1"
                     /translation="MSKILLSLPKGERVGIAFSGGLDTSAALAWMRHHGAIPYAYTAN
                     LAQPDESDYDDITRRAKLYGAEEARLVDCREALVHEGLVALQCGAFHITTAGRTYFNT
                     TPLGRAVTGTVLVKAMQEDGVNIWGDGSTYKGNDIERFYRYGLMANPQLRIYKPWLDQ
                     DFVREMGGRKEMSEWLASRNFPYKMSVEKAYSTDSNIWGATHEAKELEFLNKGLRIVQ
                     PIMSVKHWDPAVEIRPEEVTVRFEEGFPAALNGQAFASRVDLALEANRIGGRHGLGIS
                     DQIENRIIEAKSRGIYEGPGLALLHIAYERLISAIHNEGTLDNYRTMGRRLGRLLYEG
                     RWFDPQSLMLRDTLQRWVGRAITGEVAIELRRGDDYTILDTTSPNLTYHPERLSMERV
                     ADEAFSPVDRIGQLQMRNLDILDSRQKLGIYAKAGLLPSGTGAAGLLESREDKP"
     gene            2104..4653
                     /locus_tag="VFV55_06845"
     CDS             2104..4653
                     /locus_tag="VFV55_06845"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX4944055.1"
                     /translation="MLRRKRLAQSSAFATRSFKPKSLLAALGAAGLSFALATPSHAVL
                     ERFGPINNSPTVGGFPAWAQDKTGLALEFCDLMSQAEFDGGWCLLIPPGPVFPESFPI
                     NFFGEHFYFDAGASLNGSGIQANLGLALEATFLQPTPIPGDQMVFGRIRFVLRSVPVT
                     GTYTVHHPYGTWIFLDVPAGDRIFFTEDIGVACIGTFTCALDSNIGPFLLPSATPGGA
                     EVPPIPDILAGQDPYYDALVTAGAATPYPGTGKKYIADPGRLGPVTGSPLPPFLGSDG
                     LLHNHNTFRIEGPGGLVLQTDNFSLMGRVMTGALPGNVKADRASYTKLVTTPNGNNKK
                     LDVFATANPTSTGRVPAQPTPPAITPILTFFDVPCAGTVDPVTGAILPPYSKPATGNE
                     IQMVNAGNKYWGQTHPVAIPTHSCLEDSTARDAAGNVVPVFYNVKVTDEVAITALAGS
                     SGAVYDAANGGTLTVKATSSDTEVPPVLTVAGYGDMVNGTLTVTPLAAPPAKVSVVSS
                     EGGSAELLVTTNVGTAGGGGATVPLAVNDDVSMFEDCSATTATACATPLVITPLANDT
                     FQGAPITTGTVTITAQPRLGTAVLNLDNTITYTPNANVSGVEGIGYTVTVNGIVSNIA
                     YITINVAAVNDAPVAVNDATDAVVAVANSINVMANDTDVDGIADPLFGLGTIQILTWP
                     AELGPQPVPAGGVVSFTPTTSGTFTFTYNVVDNAGAASVTPATVTVTVRASETISFVR
                     SEYRASQARWTVTGADNVHAGQTLTIVYNDGTLTAAEGGGTCNGTATLPKCVLGTAVV
                     DGLGNWALDVRAATGSLNPTSASWTTRPTVVRAFSSSPVLGGSRTNSIAFR"
BASE COUNT          914 a         1832 c         1684 g          787 t
ORIGIN      
        1 cagtggctgc gctgcgggcc gccgggcgcg ggccacttcg tgaagatgat ccacaacggc
       61 atcgagtacg ggctgatgca ggcctacgcc gagggcttcg cgctgatgca gggccacccg
      121 gacttcgacc tcgacctcgc agcggtcgcc cgcacgtgga tgcagggcag cgtcgtgcgc
      181 tcgtggctgc tggagctcac gcgcgacttc ctcgccgagg acgcctcgct cgagggcgtg
      241 gcacccgtcg tcgccgattc cggcgagggg cgctggaccg cgcaggaagc catcgaccgc
      301 ggcgtgcccg cgcccgtcat caccgccgcc ctgatgcagc gcttcgcaag ccagggtggt
      361 gacgacttcg ccgcccgcgt cctggccacg atgcgaaacc gcttcggcgg ccacgccgtt
      421 ccgggcaagg acggctgaaa agggtggccg gggcggcgac gcgataaaat gtcgccacca
      481 tgagcaaaat cctcctgtcc cttcccaagg gcgagcgcgt cggcatcgcc ttctccggcg
      541 gcctcgacac ctccgccgcc ctcgcctgga tgcgccacca cggcgccatc ccctacgcct
      601 acacggccaa cctcgcgcag cccgacgaga gcgactacga cgacatcacg cgccgggcga
      661 agctctacgg cgccgaggag gcccgcctcg tcgattgccg cgaggcgctc gtccacgagg
      721 gcctggtcgc cctgcagtgc ggcgccttcc acatcaccac ggccgggcgg acgtacttca
      781 acacgacacc cctcggacgc gccgtgaccg gcaccgtgct cgtgaaggcg atgcaggagg
      841 acggcgtcaa catctggggc gacggctcca cctacaaggg caacgacatc gagcggttct
      901 accgctacgg gctgatggcc aacccgcagc tgcgcatcta caagccctgg ctggaccagg
      961 acttcgtgcg cgagatgggc gggcgcaagg agatgagcga gtggctcgcc tcgcgcaact
     1021 tcccctacaa gatgagcgtg gagaaggcct actcgaccga ctcgaacatc tggggcgcca
     1081 cgcacgaggc caaggagctc gagttcctga acaagggcct gcgaatcgtg cagcccatca
     1141 tgtcggtgaa gcactgggat cccgcggtcg agatcaggcc cgaggaagtc acggttcgct
     1201 tcgaggaggg cttcccggcc gcgcttaacg gccaggcgtt cgccagccgc gtggacctcg
     1261 cgctggaggc caaccgcatc ggcggccgcc acgggctggg catctccgac cagatcgaga
     1321 accgcatcat cgaggcgaag agccgcggca tctacgaagg gccggggctg gctcttctgc
     1381 acatcgccta cgaacgcctc atctccgcga tccacaacga aggcaccctc gacaactacc
     1441 gcacgatggg gcggcgcctc gggcgcctgc tctacgaggg ccgctggttc gacccgcaga
     1501 gcctgatgct gcgcgacacg ctccagcgct gggtgggccg cgccatcacc ggcgaggtgg
     1561 cgatcgagct gcgccgcggc gacgactaca ccatcctcga caccacgagc cccaacctca
     1621 cctaccaccc cgagcgactg tcgatggagc gcgtggcgga cgaggccttc tcgcccgtcg
     1681 atcgcatcgg ccagctgcag atgcgcaacc tcgacatcct cgattcgcgg cagaagctcg
     1741 gcatctacgc caaggccggc ctgctgcctt cgggcacggg agccgcgggc ctgctcgaat
     1801 cccgcgagga caagccgtaa cggcgcgata gcagtcgggg ccgccgcggc tgccggcatg
     1861 ccggcggcga ggtcatccgg cgccatcagg acccattgcg cacgttaacc gcacgccagg
     1921 tgcggtttat tgtcgcacct acgcttccgg tggactgcct tgttgatcca gatcaactgc
     1981 gctgcagttc gcgatgtttt ccttgaagcg caatttcgca ccacgggttg tggcgcagcc
     2041 gcagaacgga gcgacccccc cactgcggcc cttcaaggct caactgaaac ctgggagaag
     2101 aacatgttgc ggagaaagag actcgcgcag tcgtccgcgt ttgcaacgcg gagcttcaaa
     2161 cccaaatcgc tgctggccgc cctcggcgcc gcggggttgt cgtttgccct cgcgacgccg
     2221 tcgcacgcgg tcctcgagcg cttcggcccc atcaacaaca gcccgaccgt cggcggattc
     2281 ccggcgtggg cacaggacaa gaccggcctg gcgctcgagt tctgcgacct catgtcacag
     2341 gcggagttcg acggcggctg gtgcctgctc attccgcccg gcccggtgtt cccggagtcg
     2401 ttcccgatca acttctttgg ggagcatttc tatttcgatg ccggggcgag cctgaacggc
     2461 agcgggatcc aggcgaacct gggcctggct ctcgaggcga ccttcctcca gcccacgccg
     2521 ataccgggcg accagatggt cttcggccgc atccgtttcg tcctgagaag cgtgccggtc
     2581 acgggcacct acaccgtgca tcacccttac ggcacgtgga tctttctcga cgtcccggca
     2641 ggcgaccgca tcttcttcac cgaggacatc ggcgtagcct gcatcggcac cttcacctgc
     2701 gccctggaca gcaacatcgg cccgttcctc ctgccgtcgg caacccccgg tggcgcggaa
     2761 gtgccgccga ttcccgatat ccttgccggc caggacccgt actacgacgc cctggtcacc
     2821 gccggcgcgg cgaccccgta ccccggcacg ggcaagaagt acatcgccga tccggggcgc
     2881 ttgggccccg tgaccggcag cccgctgccg cctttcctcg gcagtgacgg attgcttcac
     2941 aaccacaaca cgttccgtat cgagggcccc ggcggcctcg tgctgcagac cgacaacttc
     3001 tccctgatgg ggcgcgtcat gaccggtgcg cttcccggca acgtcaaggc ggaccgcgcc
     3061 agctacacga aactcgtcac caccccgaac ggcaacaaca agaagctgga cgtcttcgcg
     3121 acggccaacc cgacctccac gggccgggtg ccggcacagc cgacgccgcc ggccatcacg
     3181 ccgatcctga ccttcttcga cgtgccttgc gccggcaccg tcgaccccgt gaccggcgcg
     3241 atcctgccgc cgtacagcaa gcccgccacc ggcaacgaaa tccagatggt caacgccgga
     3301 aacaagtact gggggcaaac gcacccggtc gcgattccga cgcattcctg cctggaggac
     3361 tccactgcgc gtgatgcggc cggaaacgtg gtgccggtgt tctacaacgt gaaggtcacc
     3421 gacgaagtcg ccatcacggc gctcgccggc tcgtccggcg cggtgtacga cgccgccaac
     3481 gggggcacgc tcaccgtcaa ggcgacgtcc agcgacacgg aggttccgcc agtcctgacg
     3541 gtggccgggt atggcgacat ggtgaacggc acgctcacgg tgacgccgct cgcggccccg
     3601 ccggcgaagg tgtcggtggt ctcgtccgaa ggcggcagcg ccgagttgct cgtcacgacc
     3661 aacgtcggaa ccgccggcgg cggcggcgca accgtgccgc tcgcggtgaa cgacgacgtg
     3721 tcgatgttcg aggactgcag cgcgacgacg gccacggcct gcgccacgcc gctggtcatc
     3781 accccgctgg cgaacgacac cttccagggt gctccgatca ccaccggcac ggtgacgatc
     3841 acggcgcaac cgcgcctcgg cacggccgtg ctcaacctcg acaacacgat cacctacacg
     3901 ccgaatgcga acgtgagtgg cgtcgagggc atcggctaca ccgtgacggt caacggcatc
     3961 gtgtccaaca tcgcgtacat cacgatcaac gtcgctgcgg tcaacgatgc cccggtcgcg
     4021 gtcaatgacg ctaccgacgc ggtggtcgca gtggccaaca gcatcaacgt gatggccaac
     4081 gacactgatg tggacggcat agcggatccg ctgttcgggc tcggcacgat ccagatcctg
     4141 acctggccgg cagaactcgg cccgcagccg gttccggccg gcggcgtcgt gagcttcacg
     4201 cccacgacct ccgggacgtt caccttcacg tacaacgtgg tggacaacgc cggggctgcg
     4261 tcggtcacgc cagcgacggt gacggtcacc gtgcgcgcgt cggaaacgat cagcttcgtc
     4321 aggtcggagt atcgggccag ccaggcccgc tggacggtca cgggcgctga caacgtacac
     4381 gccggccaga cgctcacgat cgtctacaac gacgggacgc tcacggcggc ggaaggcggc
     4441 ggaacctgca acggcacggc gaccttgccg aagtgcgtcc tcggcacggc agtcgtggac
     4501 ggcctcggca actgggcact cgacgtgcgc gccgcgaccg gctccctgaa cccgaccagc
     4561 gcgtcgtgga cgaccaggcc aaccgtggtc agggcgttct cgagctcgcc ggtccttggc
     4621 ggctcccgca ccaactcgat cgccttcagg tagcagcacc gggcggcttc aagccgccgc
     4681 aacggggggc ccgggccgca aggcccgggc ttttttttga atcttcgccg aattcctggg
     4741 acgcgatcct cgtgccggat atgtgcggcg aatgagaggt gagccggggc tggggcaagt
     4801 ccatcggcgt cctgggcacg cggcgaaagg taggtaggcc ggggatgggg caagtctatc
     4861 ggcgtcctgg gcacgcaagc gtgaccgtcg aatgggtccc aaaaatccga ggccaggcag
     4921 aacaagccag gcctcggatt tttacccatt gagagatggg gtcgcttcgc tctcccatcc
     4981 cgacggtcgc gtgcccaagg acgccgattg acttgcccca tccccggccc acatgaagag
     5041 gcagcgtggc gctttcgaca gcggatgagt aggtcgaatg tagcctcctc ggcgctgtat
     5101 ccacggagcg tgggaggcgg tggcggtgga agtcgatcgc aatccttggc acgcgaccgt
     5161 cgcggacgag aaagcgttag cgccctcgtc attagatggg tggaaagtcg aggcctg
//
LOCUS       DASUEV010000211         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_987607, whole genome shotgun sequence.
ACCESSION   DASUEV010000211 DASUEV010000000
VERSION     DASUEV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987607"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFV55_06850"
     CDS             complement(<1..288)
                     /locus_tag="VFV55_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX4944056.1"
                     /translation="MATRKTKSRARAGKTVDDTQLGKAVTHSAQKIWLAGLGAFARAR
                     TEGDKLFDLLVEQGKVIRSKSGKAADQALKTVRSQADATLSTAQGKWDKLEQ"
     gene            512..2191
                     /locus_tag="VFV55_06855"
     CDS             512..2191
                     /locus_tag="VFV55_06855"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX4944057.1"
                     /translation="MERPWIKSYPPGVPADIDVDAYASIRDILETSCARFADLPAFHC
                     MGATMSYRELDRLSTDFGSWLQNVAGLGKGARVAVMMPNLLQYPVAIFGLLRAGLIGV
                     NCNPLYTPRELEHQLNDSGAEAIVILENFAKTLEEVIGRTRVKTVVTTRIGDQLGFPK
                     SLITNFVVKRLKKMVPPFSLPGAVGFRDALAAGRSHRLAPPPLGHDDIAFLQYTGGTT
                     GVSKGAMLTHANLVANLLQVSAWVDTGLEEGGETVITALPLYHIFALTGNCLTFMKIG
                     AKNVLIPNPRDLPGFVAELRKHRFTAITGVNTLFNALMNTPGFAQLDFSQLKMALGGG
                     MAVQRAVAERWLEITGTPLIEAYGLTETSPAACINPLVKGARYNGTAGLPVPSTTVTI
                     RDEAGNTLPIGQTGEICIAGPQVMKGYWNRPDETAKVMFADGAFRTGDIGLMDERGYV
                     KIVDRKKDMILVSGFNVYPNEVEDVVALMPGVLEVCAVAAADDKSGEVVRLVIVRKDP
                     ALTKDAVLVHCRKHLTGYKLPKIIEFWKELPKTNVGKVLRREVKNTPVEGA"
     gene            complement(2188..3390)
                     /gene="rocD"
                     /locus_tag="VFV55_06860"
     CDS             complement(2188..3390)
                     /gene="rocD"
                     /locus_tag="VFV55_06860"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX4944058.1"
                     /translation="MGDMIALERRYAARNYDPLPVVLTEGAGAWLTDEQGRRYLDMLS
                     AYSAVSFGHAHPRLVAVLTRQAGRLAVTSRVFHNDRLPPFLARLCQLTGMDRALPMNT
                     GAEAVETAIKCARKWAHEVKGVPDGHAQIVVCENNFHGRTSTIVGFSSEPQYRRGFGP
                     FGPGFRHVPFGDSRALEEAIGRDTAAFLVEPIQGEAGVVVPPAGYLAQCAAICRHHGI
                     LLLVDEVQTGLGRTGRLLACEHEGVRPDGLVLGKALGGGLLPVSAFLANDAVMGVLRP
                     GDHGSTFGGNPLAAAVGLEALDVLVEERLSERAAALGEDFLARLRGIDSPIVREVRGR
                     GLLVGMEIDPARSTARRVVDCLLARGLLSKDTRGTVVRFAPPLVIGREELDWAMPRIR
                     EALAEAAG"
     gene            complement(3516..>4708)
                     /locus_tag="VFV55_06865"
     CDS             complement(3516..>4708)
                     /locus_tag="VFV55_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4944059.1"
                     /translation="LAPWFDRMEERLSIAPWPVAPNENNDILKRGCEKLGIPAAAIRR
                     NVRGCWNLGYCGMGCPTNAKQSMLVTTIPSALSRGATLVHRARIERLVATGGRITAAE
                     ARGVAGSGTPPGPQRIRLAARHFILSSGAIGTPALLLRSGLPDPYELAGKRTFLHPTV
                     VSAAVMPSPVKGYEGAPQTIYSDHFLDSVPVGGPVGYKLEAPPLHPILAGITLPGYGS
                     GHARWMKEFANLQVVIALLRDGFHPESPGGRVVLRADGSPVLDYPLGDFLMEGMRRAY
                     LTMAEIQFAAGAKTVMALHEDAAAMTSWPNARAAIAALPMAALRARVVSAHVMGGCAM
                     GPDARRAVVDERGRHHHVANLSIHDGSLFPTSLGANPQLSIYALSARMATELARELVP
                     GHAD"
BASE COUNT          754 a         1567 c         1659 g          728 t
ORIGIN      
        1 ctgctcgagc ttgtcccact tgccctgcgc cgtggacagc gtggcgtcgg cctgcgagcg
       61 caccgtcttc agcgcctggt cggcggcctt cccgctcttc gagcggatca ccttgccctg
      121 ctcgacgagc aggtcgaaca acttgtcgcc ctcggtgcgg gcacgtgcga acgcgccaag
      181 tcccgcgagc cagatcttct gcgccgagtg ggtgactgcc ttccccaatt gggtgtcgtc
      241 gacggtcttg ccggccctgg cgcgagattt cgttttccgg gttgccatgc gttcctcctg
      301 gaattgccgg cgccgtgccc ccgtcgggcg ggccaccgtg atcatgctga aaagtctagg
      361 cggcaggatt agagcaatca cactattctt cgccgcagcc tgccgtaagg tagacttcac
      421 ccgccgattc cggcgacggc agttgcgggt tacggcgcga gggcaaaacg ggcaccgggg
      481 acgacgagct ccgggccagc gcgaggagaa catggaacga ccctggatca agtcctatcc
      541 ccccggcgtg ccggccgaca tcgacgtcga tgcctacgcg agcatccgcg acatcctcga
      601 gacgagctgc gcccgttttg ccgacctgcc ggcgttccac tgcatgggcg ccacgatgtc
      661 ataccgggaa ctcgatcgcc tttccacgga tttcggcagc tggctgcaga acgtcgcggg
      721 gctcggcaag ggcgcgcggg tggcggtgat gatgcccaac ctgctgcagt atcccgtggc
      781 gatcttcggc ctgctccggg cggggctgat cggggtgaac tgcaatcccc tgtacacgcc
      841 ccgcgagctc gagcaccagc tgaacgactc cggcgccgag gcgatcgtga tcctggagaa
      901 cttcgcgaag acgctcgagg aggtcatcgg ccggacccgg gtgaagaccg ttgtcacgac
      961 ccggatcggc gaccagctgg gctttccgaa atcgctcatc accaatttcg tggtgaagcg
     1021 gctgaagaag atggtgccgc cattcagcct gcccggcgcg gtggggttcc gcgacgcgct
     1081 cgccgcgggc cgctcgcacc ggttggcgcc gccccccctc ggccatgacg acatcgcgtt
     1141 cctccagtac acggggggaa cgaccggtgt ttcgaagggc gcgatgctta cgcacgcgaa
     1201 cctggtggcc aacctgctgc aggtttccgc ctgggtggac accggcctcg aggaaggcgg
     1261 cgagacggtg atcacggcgc tgccgctgta ccacatcttc gcgctcaccg gaaactgcct
     1321 caccttcatg aagatcggcg cgaagaacgt cctgattccc aatccgcgtg acctgccggg
     1381 attcgtggcc gagttgcgca agcaccgttt cacggcaatc accggcgtga atacgctgtt
     1441 caacgcgctc atgaacacgc cgggattcgc gcagctcgat ttctcgcagc tcaagatggc
     1501 cctcggcgga ggcatggccg tgcaacgtgc cgtggccgag cgctggctgg agatcaccgg
     1561 gacgccgctc atcgaggcct acggcctcac cgagacgtcg ccggcggcct gcatcaatcc
     1621 gctcgtgaaa ggcgcccggt acaacggcac ggcgggactc ccggtgccgt cgacgaccgt
     1681 caccatccgc gacgaggccg gcaatacgct gcccatcggc cagacgggcg agatctgcat
     1741 cgccggcccg caggtgatga aaggctactg gaaccggccc gacgagacgg cgaaggtgat
     1801 gttcgcggat ggcgcgttcc ggacgggaga catcggcctc atggacgagc gcggctacgt
     1861 gaagatcgtg gaccgcaaga aggacatgat cctcgtctcc ggattcaacg tttacccgaa
     1921 cgaggtcgag gacgtggtgg cgctgatgcc gggcgtcctc gaggtgtgcg cggtggccgc
     1981 cgccgacgac aagtccggcg aggtggtccg gctggtgatc gtgaggaagg atccggcgct
     2041 cacgaaggat gcggtcctcg tgcactgcag gaagcacctc accggctaca agctccccaa
     2101 gatcatcgag ttctggaagg agctcccgaa gacgaacgtg ggcaaggtcc tgcgccgcga
     2161 ggtcaagaac acgcccgtgg agggggctta gccggccgcc tcggcgagcg cctcgcggat
     2221 gcgcggcatc gcccagtcga gttcctcgcg cccgatgacg agcggcggag cgaagcgcac
     2281 caccgtcccc cgcgtgtcct tgctgagcag gccgcgcgcc aggaggcagt ccacgacccg
     2341 gcgcgccgtg gagcgggccg ggtcgatctc catccccacg agcaatcccc ggccgcgaac
     2401 ctcgcgcacg atcggcgagt cgatcccgcg caggcgggcg aggaaatcct cgccgagtgc
     2461 ggcggccctc tcggacaatc gctcctcgac gagcacgtcg agcgcttcga gcccgaccgc
     2521 cgcggccagc gggtttcccc cgaacgtgct gccgtggtcc ccggggcgca ggacgcccat
     2581 gacggcgtcg ttcgcgagaa aggcggaaac cggcaggagg ccgccgccga gcgccttgcc
     2641 gaggacgagc ccgtcgggcc gcacgccctc gtgctcgcag gcgagcagcc gccccgtgcg
     2701 cccgagcccc gtctgcacct cgtcgaccag gagcaggatg ccgtggtgcc tgcatatcgc
     2761 ggcgcactgc gcgaggtagc ctgcgggagg gaccacgacg cctgcttcgc cctgaatcgg
     2821 ctcgacgagg aacgcggccg tgtcgcggcc gatcgcctcc tcgagcgcgc gagagtcgcc
     2881 gaacgggaca tggcggaagc cgggcccgaa cgggccgaag ccgcgccggt actgcggctc
     2941 ggaagaaaag ccgacgatgg tcgaggtccg gccgtggaag ttgttctcgc agaccacgat
     3001 ctgcgcatgg ccgtccggaa cgcccttcac ctcgtgcgcc cacttgcgcg cgcacttgat
     3061 cgcggtctcc actgcctcgg cgccggtgtt catcggcaac gcacggtcca tgccggtcag
     3121 ctggcaaagg cgcgcgagga agggcggcag gcggtcgttg tggaatacgc gcgaggtgac
     3181 ggcgaggcgt cccgcctgcc gcgtgaggac ggccaccagg cgcgggtgcg cgtggccgaa
     3241 gctcacggcg gaataggccg acagcatgtc gaggtaacga cgcccttgct cgtcggtgag
     3301 ccaggcaccg gcgccctcgg tcaggacgac gggcagcgga tcgtaattgc gcgccgcata
     3361 gcggcgctcg agtgcaatca tgtcgcccat ggcggttccc cgatctcgct cgtggcaagt
     3421 aaggggcctc caggcccggc ggcgtttccc accaggcccg gcggcgcttc gtcgggacag
     3481 tatatcggga cggcgcgacg cgcttgggga ttgcctcagt ccgcgtggcc tggcacaagc
     3541 tcgcgggcga gttcggtcgc catgcgggcg ctgagcgcgt agatcgacag ctgcggattg
     3601 gcaccgaggc ttgtggggaa aagggacccg tcgtgaatcg acaggttcgc gacatgatgg
     3661 tggcggccgc gctcgtcgac caccgcgcgg cgtgcgtccg gtcccatggc gcatcctccc
     3721 atcacgtgcg cgctcaccac gcgggcgcgc agcgcagcca tcgggagcgc cgcgatggcg
     3781 gccctcgcgt tcggccagga ggtcatcgcg gccgcgtcct cgtgcagggc catgaccgtt
     3841 ttcgctcccg cggcgaactg gatttcggcc atcgtgaggt aggcgcggcg catgccctcc
     3901 atgaggaaat cgccgagggg atagtcgagc acgggagagc cgtccgcgcg caacaccacg
     3961 cggccgcccg gcgattcggg atggaacccg tcgcgcagca gcgcgatgac cacctgcagg
     4021 tttgcgaact ccttcatcca ccgcgcgtgc ccggacccgt agcccggaag ggtgatgccg
     4081 gcgaggatcg gatgcagggg cggggcttcc agcttgtagc cgaccggccc gccgacgggg
     4141 acgctgtcga gaaagtggtc cgagtagatc gtttgcggcg cgccttcgta acccttcacg
     4201 ggcgagggca tcacggccgc cgacacgacg gtggggtgca ggaaggtgcg ttttcccgcg
     4261 agttcgtagg ggtcgggcag gccgcttcgc agcagcagcg cgggggtgcc gatggcgccc
     4321 gacgagagga tgaaatggcg ggccgcgagg cggatgcgct gcggtccggg gggtgtaccc
     4381 gatcccgcga cgcctcgtgc ttcggcggcc gtgattcgtc cgccggtggc gacgagccgt
     4441 tcgatgcggg cgcgatgcac gagggtggcg cccctggaca gggccgacgg gatcgtcgtc
     4501 accagcatcg attgtttcgc gttcgtaggg cagcccatgc cgcagtagcc gaggttccag
     4561 cagcctctta cgttgcgccg gatggcggcg gcggggatgc ccagcttctc gcagccgcgc
     4621 ttcaggatgt cgttgttctc gttgggcgcc acgggccagg gcgcgatgga gaggcgctct
     4681 tccatgcggt cgaaccaggg cgcgaggg
//
LOCUS       DASUEV010000212         6093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_740179, whole genome shotgun sequence.
ACCESSION   DASUEV010000212 DASUEV010000000
VERSION     DASUEV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740179"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="htpG"
                     /locus_tag="VFV55_06870"
     CDS             <1..1176
                     /gene="htpG"
                     /locus_tag="VFV55_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX4944060.1"
                     /translation="YREFYKHVGHDFDDPLAWTHARVEGRQEYTHLLYIPARAPFDLW
                     DRNARHGVKLYVRRVFIMDDAEQLLPAYLRFVRGVVDSNDLPLNVSREILQESKDIEA
                     IRGGCTRKVLGLLETLAKDEKDKYAEFWKQFGRVLKEGIGEDFANKEKIAGLLRFAST
                     HADTADETVSLADYLARAREGQDKIYYVTAETFNAAKNSPHLEVFRKKGIEVLLLSDR
                     VDEWVVSHLTEFEGKELDSVARGGLDLGVLADEGERKAVEQDATELKDLIARIKASLG
                     DRVKEVRVTHRLTESPACLVADEHDVSGNLARLLKAAGQGVPASKPILEINPKHPVVL
                     RLRGEEKKFDDWAAVLFDQALLAEGGQLDDPAGFVKRINGLMLEMGGAGESRIVLPG"
     gene            complement(1115..1912)
                     /locus_tag="VFV55_06875"
     CDS             complement(1115..1912)
                     /locus_tag="VFV55_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4944061.1"
                     /translation="MAAELARESRVACPDVAGRGESDRLASAFQYHFPQYLSDIHALL
                     AQLGVKKLDWVGTSMGGLLGMMLAAQPGSPIRRLVMNDVGAFLPMGALQHIGRNLEAP
                     ATFASLAEVETHMRHTHRDWGEITDAQWRHLAIHGARLVDGEYRLHFDPQLATPFQSF
                     AVGPGLFFWDKWAQVRCPVLLIRGEHSDVFPRSVADLMLTMNPGARLVEIPGCGHAPS
                     LMAPSQIAIVQEFLAKPDKAASRAKASLTRATRSATRLLRPSPASAR"
     gene            complement(2111..2332)
                     /locus_tag="VFV55_06880"
     CDS             complement(2111..2332)
                     /locus_tag="VFV55_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944062.1"
                     /translation="MDYKTSFPEYQEIEKIIAQARLERSAYLGGKIASAIVAVRDAVR
                     GFMDRSPLRGSRIPWGPDDQLPNRTIAWD"
     gene            2553..3344
                     /locus_tag="VFV55_06885"
     CDS             2553..3344
                     /locus_tag="VFV55_06885"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX4944063.1"
                     /translation="MKRVLGLSLFLVVAPASQAADIEAGKAKVATVCAACHGTNGVSV
                     SDNIPNLAAQRAVYIEVQLKALKEGARKNPIMNAIAGQLGAEDMANVAAYFASLPGPA
                     STAKSELLPNLVKSNVRFPEGYKDSFVKYHTINFPATKQVRYYFANKAAAQAARDGKP
                     LPDGSVLFAEVYSAKLGDDQKPVLGADGFFVADKLLIYTAMEREAGWGNDIPEMLRND
                     NWNYAVFTTDKQQRPGVNQAECLACHKPFDKASYTFTLKQLAAAK"
     gene            complement(3313..>6093)
                     /locus_tag="VFV55_06890"
     CDS             complement(3313..>6093)
                     /locus_tag="VFV55_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4944064.1"
                     /translation="TRSTCCYCGVGCGVVIESRDGAITGVRGDPDHPANFGKLCSKGT
                     TLHLTASPAASAGRARFPELRRDRASERERVTWDEALDHAAERFAAVIRDHGPDAVAF
                     YVSGQLLTEDYYAFNKLAKALVGTANIDTNSRLCMSSAVTGYQQTLGTDAPPCSYEDL
                     DLAHCFFIAGSNMAHAHPVLFRRIEAARAANPALKVIVVDPRRTETARTADLHLAILP
                     GTDVALFHAMLHVMLWEDLVDRAYVDAHVEGFDALRAAVRDWTPQAAADVCGVKAADI
                     ATAARWFAALPATLSLYCQGLNQSASGTAKNAALVNLHLATAQIGKPGAGPFSLTGQP
                     NAMGGREVGGLATSLAAHRSLGNADDRAEMARLWGVERVPDKPGKTAIEMFEALGRGE
                     IKAVWIACTNPAQSLPDLPSVRKALQRAELVVLQEAYRDTETAAFADLLLPATSWGEK
                     EGTVTNSERRISRVRAAVAKPSGARHDWEIAVDFARRLEARIRPGKPTLFAFDSAESL
                     WNEHRETTRGRDLDITGLSWEILESRGPQQWPFHEGAGEGRARLYEDGNYPTASGRAR
                     FFTGAYKPTAEKADAKFPLRLTTGRLRDQWHGMSRTGNVPALFGHVAEPRLGMHSADM
                     ARRGIHDGDLVRAESRRGGLFVIAEASDDLRSGQAFLAMHWGKRFLGGAASDGINTLT
                     IAAIDPDSRQPELKHAAVRVEPAALAWRLVAFAEVAADRLAGTLERLRALQDCVAFHS
                     VVPAGRERAGLLVRSAHAGEPPAAWLAELDAALGLDGEDAIRYDDPRRASSRRLRIAG
                     DRLTAVRLSGEVDALASGEWLRAWLLEGRPVADIRRHLLIPTRQAPSGFVPAGRIVCQ
                     CFGVTEAAIEASLAATQGAPGERLATLQAKSKCGTNCGSCLPEIRRLANLPQRQAPDT
                     LGLISLPRAASA"
BASE COUNT         1015 a         2045 c         2117 g          916 t
ORIGIN      
        1 tacagggaat tctacaagca cgtcgggcac gacttcgacg acccgctcgc gtggacccat
       61 gcgcgcgtcg aagggcggca ggagtacacg cacctgctct acatcccggc ccgcgccccg
      121 ttcgacctgt gggaccgcaa cgcgcgccac ggcgtgaagc tctacgtgcg ccgcgtgttc
      181 atcatggacg atgccgagca gctcctgccg gcgtacctgc gcttcgtgcg cggcgtggtc
      241 gattccaacg acctgccgct caacgtctcg cgcgagatcc tgcaggagtc gaaggacatc
      301 gaggccatcc gcggcggctg cacgcgcaag gtgctcggcc ttctggagac cctggccaag
      361 gacgagaagg acaagtacgc cgagttctgg aagcagttcg gccgcgtgct caaggaaggc
      421 atcggcgagg acttcgccaa caaggagaag atcgcgggcc tgcttcgctt cgcctcgacc
      481 cacgccgaca ctgccgacga gacggtctcg ctcgcggact acctcgcgcg cgccagggaa
      541 ggccaggaca agatctacta cgtgactgcg gagaccttca acgccgcgaa gaacagcccg
      601 cacctcgagg tgttccgcaa gaagggcatc gaggtgctgc tgctttccga ccgggtggac
      661 gagtgggtcg tgagccacct caccgaattc gagggcaagg agctcgactc cgtggcgcgc
      721 ggcgggctcg atctcggcgt gctggccgac gaaggcgaac gcaaggccgt ggagcaggac
      781 gcgaccgagc tcaaggacct catcgcgcgc atcaaggcga gcctcggcga tcgtgtcaag
      841 gaagtgcgcg tcacgcaccg cctcacggag tcaccggcct gcctcgtcgc ggacgagcac
      901 gacgtgagcg gaaacctcgc gcggctcctc aaggcggcgg ggcagggcgt gcccgcctcc
      961 aagccgattc tcgagatcaa cccgaagcat cctgtggtgc tgcgcctcag gggcgaggag
     1021 aagaagttcg acgactgggc ggcggtgctc ttcgaccagg cgctcctcgc cgaaggcggc
     1081 cagctcgacg acccggccgg gttcgtgaag cggatcaacg ggctgatgct ggagatgggc
     1141 ggagcaggcg agtcgcggat cgtgttgccc gggtaaggct cgcctttgcc cgcgacgccg
     1201 ccttgtccgg tttcgccagg aattcctgca cgatggcgat ctgcgacggc gccatgagcg
     1261 acggcgcatg gccgcaaccg ggaatctcga ccaggcgcgc gccgggattc atcgtgagca
     1321 tcaggtccgc cacggagcgg ggaaacacgt ccgaatgctc gccccggatg aggaggacag
     1381 ggcagcgcac ctgcgcccac ttgtcccaga agaacagccc cggccccacg gcgaaggact
     1441 ggaagggcgt ggcgagctgc gggtcgaaat gcaggcggta ttcgccgtcc acgagccttg
     1501 cgccgtggat cgcgagatgg cgccactggg catcggtgat ctcgccccag tcgcggtggg
     1561 tatggcgcat gtgcgtctcg acctccgcca gcgaggcgaa cgtcgccggc gcctccaggt
     1621 tgcggccgat atgctggagc gcgcccatcg gcaggaacgc gccgacgtcg ttcatgacca
     1681 gccggcggat ggggctgccg ggttgcgcgg cgagcatcat gccgagcagc ccgcccatgg
     1741 acgttcccac ccagtccagc ttcttcacgc ccaattgcgc gagaagcgcg tggatgtccg
     1801 aaaggtattg cgggaagtgg tactggaacg cgctggcgag ccggtcgctc tcgccgcggc
     1861 cggcgacgtc ggggcaggct acgcgcgact cgcgcgcgag ctccgcggcc aggaaatcga
     1921 agtcgcgcga attgccgctg tagccgtgtg cgcagacgac gacccgcgcg ttgtccggag
     1981 cgccccactc gacgtaatgg aggttgtggt atcccgcccc gctgacgccg cgaaacgcgt
     2041 ggtggcgggg ctcggcaaca tgcccttcat gatccggctg cggtgacctg gcgaggacct
     2101 tcggttcgag tcaatcccag gcaatagtcc gattggggag ctggtcgtcg gggccccacg
     2161 gaatccggct gccacgaagc gggctgcggt ccatgaaccc ccgcacggcg tcgcgaacgg
     2221 cgacaatcgc gctcgcgatc ttcccgccga ggtaagcgct gcgctcgagg cgcgcctgcg
     2281 cgatgatctt ctcgatctcc tggtattccg gaaaagacgt cttgtagtcc atagcagact
     2341 ccttttggga gattgggagg ggacccgcgt aacggatggc gttacagtta cggaccctgt
     2401 cacgacaaca tagggatccc gccctcccct gacaaaccgg aaaatctgat gtgacgcctc
     2461 acaaaatcta atccatgccc cgcccggaat gaccccgccc cggaaagtgt tcaccccatt
     2521 gccgcccaat cacccgccac aggagaagcc ccatgaaaag agtgctcgga ttgtcccttt
     2581 ttctcgtcgt agcgccggct tcgcaggcgg ccgacatcga ggccggcaag gcgaaggtcg
     2641 ccacggtctg cgcggcgtgc cacggcacca acggggtgag cgtgagcgac aacatcccca
     2701 acctcgcggc gcagcgggcg gtttacattg aggtccagtt gaaggcgctc aaggagggcg
     2761 cgcgcaagaa cccgatcatg aatgcgatcg ccggccagct gggcgcagag gacatggcca
     2821 acgtggccgc ctacttcgcc tcgctgcccg gccccgcgtc gaccgcgaag tcggagctcc
     2881 tgcccaacct cgtgaagtcc aatgtcaggt ttccggaagg ctacaaggac tcgttcgtga
     2941 agtaccacac gatcaatttc cccgcgacga agcaggtgcg ctactacttc gccaacaagg
     3001 ccgccgcgca ggctgcccgc gatgggaagc ccctgcccga cggctcggtg ctgttcgccg
     3061 aggtgtattc ggccaagctc ggcgacgacc agaagccggt gctgggcgcc gacggattct
     3121 tcgtggcgga caagctgctg atctacacgg cgatggaacg ggaagcgggg tggggcaacg
     3181 acatcccgga gatgctgcgc aacgacaact ggaattacgc cgtcttcacc acggacaagc
     3241 agcagcgccc cggcgtgaac caggcggaat gcctggcctg ccacaagccg ttcgacaagg
     3301 cgagctatac gttcacgctg aagcagctcg cggcagcgaa ataagcccca gggtgtccgg
     3361 ggcctgccgc tggggcaggt tggcaaggcg ccggatctcc ggcaggcacg acccgcagtt
     3421 cgtgccgcac ttcgactttg cctgcaaggt ggcgagcctt tcgcccggcg cgccttgagt
     3481 ggccgcaagc gaagcttcga tcgccgcttc ggtcacgccg aagcactggc acacgatgcg
     3541 gcccgcggga acgaagccgc tcggtgcctg gcgcgtcggg atgagaaggt ggcggcgaat
     3601 atcggccacc ggccgcccct ccaggagcca ggcgcgcagc cactcgccgc tcgccagcgc
     3661 atccacttcc cccgagaggc gcacggccgt caggcggtcg cccgcgatgc gcagccgccg
     3721 cgaggaggcg cggcgcgggt cgtcgtagcg gatggcgtcc tcgccatcga gaccgagcgc
     3781 ggcgtcgagt tcggccagcc acgcggccgg gggctcgccg gcatgggcgg accgcacgag
     3841 caaccctgcg cgttcgcgtc ccgcgggcac cacgctgtgg aaggctacgc agtcctgcaa
     3901 cgcacgaagt cgttccagag tcccggcaag ccggtccgcc gcaacctccg cgaaggcgac
     3961 cagccgccac gcgagcgccg cgggttccac cctcacggcc gcgtgcttga gctcgggctg
     4021 gcgcgagtcg ggatcgatgg ccgcgatcgt gagcgtgttg atgccatcgc tcgcggcacc
     4081 gccgagaaag cgcttgcccc agtgcatcgc caggaacgcc tgacccgaac gcaggtcgtc
     4141 cgaggcttcg gcgatcacga acaggccgcc gcgccgcgac tcggcgcgca ccaggtcgcc
     4201 gtcgtggatg ccgcgccgtg ccatgtccgc cgaatgcatg ccgaggcgcg gctcggcgac
     4261 gtggccgaag agggcgggca cgttgccggt gcggctcatg ccgtgccact ggtcgcgcag
     4321 ccgcccggtg gtcaggcgca ggggaaactt cgcgtccgcc ttctctgccg tcggcttgta
     4381 ggcgccggtg aagaaccgcg ccctgccgct cgccgtcgga tagttgccgt cttcgtagag
     4441 ccgcgcgcgt ccttcgccgg cgccttcgtg aaacggccac tgctgcggtc cccgggattc
     4501 gaggatttcc caggagaggc cggtaatgtc gagatcgcgg ccgcgcgtcg tttcgcggtg
     4561 ctcgttccac agcgactcgg ccgaatcgaa tgcaaagagc gtcggcttgc cggggcggat
     4621 gcgcgcctcc aggcgtcggg cgaagtccac cgcgatttcc cagtcgtgcc gcgcccccga
     4681 aggcttcgcg acggccgcgc gcacgcgcga gatgcgccgc tcggagttcg tgaccgtgcc
     4741 ctccttctcg ccccagctcg tggcgggcag gagcaggtcg gcgaaggccg cggtctcggt
     4801 atcgcgatac gcctcctgca gcacgacgag ctccgcgcgt tgcagcgcct ttcgcaccga
     4861 gggcaggtcg ggcagcgact gcgcggggtt ggtgcacgcg atccagaccg ccttgatttc
     4921 gccgcggccc agggcctcga acatctcgat ggcggtcttg ccgggcttgt ccggcacgcg
     4981 ttccacgccc cataggcgcg ccatctccgc ccggtcatcc gcattgccga gtgaccggtg
     5041 cgcggccagc gatgtcgcga gaccgcccac ctcgcgccca cccatggcgt tgggttgccc
     5101 cgtgaggctg aacggtccgg caccgggctt gccgatctgg gcggtggcca agtgcaggtt
     5161 gaccagggcc gcgttcttgg ccgtgccgct ggccgactgg ttgagtccct ggcagtagag
     5221 ggagagcgtg gcgggcaagg cggcgaacca gcgcgcggcg gtcgcgatgt ccgcggcctt
     5281 cacgccgcac acgtcggccg ccgcctgcgg ggtccagtcg cgcacggcgg cgcgcagcgc
     5341 gtcgaagcct tcgacatggg catcgacata cgcgcgatcg acgaggtctt cccagagcat
     5401 cacgtgcaac atcgcgtgga aaagcgcgac atcggtgccg ggcaggatgg cgaggtgcag
     5461 gtcggcggtg cgcgccgtct ccgtgcggcg cgggtcgacg acgatcacct tgagcgccgg
     5521 gttggcggcg cgtgcggcct cgatgcgccg gaagagcacc gggtgcgcgt gcgccatgtt
     5581 ggagcctgcg atgaagaagc aatgcgcgag gtcgaggtcc tcgtagctgc atgggggcgc
     5641 atcggtcccc agcgtctgct ggtaacccgt caccgcggac gacatgcaca ggcgcgaatt
     5701 ggtgtcgatg ttcgccgtgc cgacgagcgc cttggccaat ttgttgaagg cgtagtagtc
     5761 ctcggtgagg agctggccgg agacgtagaa cgcgaccgcg tcgggcccgt gatcgcggat
     5821 caccgcggca aagcgctcgg cggcgtggtc cagcgcctcg tcccaggtca cgcgctcgcg
     5881 ctccgatgcg cggtcacggc gaagttcggg gaagcgcgcc cggccggcgc tggccgcggg
     5941 actcgccgtc aggtgcaggg tcgtgccctt ggagcagagc ttgccgaaat tggccggatg
     6001 gtccgggtcg ccacgcacgc ccgtgatggc gccgtcgcgg ctctcgatca cgacgccgca
     6061 gcccacgccg caatagcagc aggtggagcg cgt
//
LOCUS       DASUEV010000213         6690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_507883, whole genome shotgun sequence.
ACCESSION   DASUEV010000213 DASUEV010000000
VERSION     DASUEV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507883"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1386)
                     /locus_tag="VFV55_06895"
     CDS             complement(199..1386)
                     /locus_tag="VFV55_06895"
                     /inference="COORDINATES: protein motif:HMM:NF022514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HEX4944065.1"
                     /translation="MARTRSKSAPRLTRDAERLIALANGLHTSGSLTEDRFWEGEIAA
                     LLEKLLEHGNDAPLDGALDHLWQTNSGAYDGLIERVEGISESVTVMQGDFAWESLLIA
                     APVVAWSKYAIPSGPIDRDDAQALKVQLQAHVLAADTRVTLVPYLYSIDQLPRHFSEL
                     RRLAAKLGDAAVTGEIPRIDFARMPETAQLLADSRFVIASVATPRGKPLFRWQEDASG
                     HTGRTTSLEQWIAQARPTIAKMLPGCVFECLLPDAYYVNCREADRRVRPYAIRSGVAF
                     LESTLHVEAAQLRAVIAGFGEERVDEYRVGFTVDGDSHVAHGVVWPLYGREDENARPG
                     PLEDLLAELTQCGVTEVKKLSGTFEPEYCEDCGAPLFANPEAEMVHAELPEEADSPAA
                     HFH"
     gene            1481..1870
                     /locus_tag="VFV55_06900"
     CDS             1481..1870
                     /locus_tag="VFV55_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEX4944066.1"
                     /translation="MPHLLNVLGGELRACCFDPLTGYYRDGSCREAEDDTGTHLVCAR
                     MTLEFLAFSLARGNDLVTPRPEMRFRGLKPGDRWCVCALRWLEALEAGVAPPVVLEAT
                     HERALDLVPLEELRRHAFDSSPSRESP"
     gene            complement(1890..2057)
                     /locus_tag="VFV55_06905"
     CDS             complement(1890..2057)
                     /locus_tag="VFV55_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944067.1"
                     /translation="MRIPTPFEIDRRAHELRREELSKLLDDAACAVRKALARFLHRSA
                     PVCHPRTRLAA"
     gene            2208..3125
                     /locus_tag="VFV55_06910"
     CDS             2208..3125
                     /locus_tag="VFV55_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4944068.1"
                     /translation="MGIPSDMAAFVRAVERGGFSSAARELGLTPSAISKLVTRMEGRL
                     GVRLLNRTTRRLALTPEGEAFFHRAQRIIADIEEAEDEVARFRERPRGKLRLNVGTAF
                     GTYQLVPALPEFLARYPEIEVEITMTDRIVDLVEEGADVGLRTGTLSDSSLVARRICD
                     MERVICASPGYLRKHGTPRSPADLSRHNCLVLAAAPQFHRWPFDYPDGVRNIEVAGNV
                     TANSAETLAQLAIVGLGLVRLVDVIVGSAIAKGLLKPVLADVHHVEPLPLHAVFPHGR
                     HRSPRVAALVDFLVEKFAHAPWRVRAGRR"
     gene            3122..4600
                     /locus_tag="VFV55_06915"
     CDS             3122..4600
                     /locus_tag="VFV55_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX4944069.1"
                     /translation="MTILAPAVAFVDLETTGTSATGDRVTEVAIVRVVEGEVVEEWCT
                     LVDPERSIPPDIQVLTGITNEMVKGAPTFADIRHEVRERLEGYLFVAHNARFDYGFLK
                     NEFARLEMPFTADVLCTVRLSRRLYPEAVGHSLDALIARHGLADPLDCADPAARTGRH
                     SALGDARALRRFTQVIEREKSGDEIAAAVKRLLKIPSLPPQLPPDALDGIPEGPGVYR
                     FFGVNDLPLYIGKSVNLRDRVRSHFTSDYRNSNDHRLSAEIRRLDWLETAGELGALLL
                     ESRLVKEVAPLHNALLRRRRTSCVLRLPSLREAPEFLDLDEIDWDARGPGAESLYGPF
                     SSPRAARDLLEAIASEEGLCWRLLGWEKRGGPCFARQVKKCRGACVGEESPEAHNVRL
                     ATALEVKRIPDWPFAGPVAIVERDPARGIEEAHVIRQWRHLGTARDEDELASLAAMRR
                     LPAFDPDILRVLVRWLAEHPGKARLLDGEPDRDIAEYADGSC"
     gene            complement(4597..5268)
                     /locus_tag="VFV55_06920"
     CDS             complement(4597..5268)
                     /locus_tag="VFV55_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX4944070.1"
                     /translation="MCSRYFLDADGNVIAYTFNVPVHDRIRKRFNIAPTQEAPVVRTS
                     EAGAREVAMLRWGLVPPWAKDLGTGSKMIMARSETVAEKPSFRDAFRQRRCLVPASGF
                     YEWTGAAGRKIPHAITFADQPVFAFAGLWERWHDKANPDAPPVETYTLITTTSNRALA
                     AIHDRMPVVLSRADHETWLSAPAEEAALLLRPYADGQTQERVVSTRVNNPRNESPDLL
                     AAGEG"
     gene            5331..6053
                     /locus_tag="VFV55_06925"
     CDS             5331..6053
                     /locus_tag="VFV55_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944071.1"
                     /translation="MQMPLACPSCQAPTDQRRFARQVHGEVILDICWDCHAFWFDQYE
                     STQLAPAAVIELFRLIHQHRAAPARPLADSMACPHCRARLLVTQDIQRTNRLTYHRCP
                     NGHGRLTTFFQFLREKQFIRSLSQPEIERLKATVSQVRCSGCGAPVDVARDAACSFCR
                     SPLSVLDSEAVERTLAALTQADRRRAPPDSAAMAEAFESLLSTHQRAAQGSPWTRDIA
                     PLQSTPAMIDLVVEGIGWLLRT"
     gene            6068..6496
                     /locus_tag="VFV55_06930"
     CDS             6068..6496
                     /locus_tag="VFV55_06930"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX4944072.1"
                     /translation="MNPTRFLRACAFVAACALLGACQIAPVKREPPPLPFTGTKWMMV
                     TERKPAGEAPYLEFGDGAVTGSSGCNRIMGRYMQDAVGAGAIVFSSIATTKRMCGDAS
                     MAVEERLLSVLRTSTNVKVTGDTLRIDGSAGSLDFSAATR"
     gene            complement(6477..>6690)
                     /locus_tag="VFV55_06935"
     CDS             complement(6477..>6690)
                     /locus_tag="VFV55_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944073.1"
                     /translation="RDAATVAGALGGALLGNEVEKNKGAVTAWDVRVRLEDGSNKVVR
                     FNSEPSWRVGDRVRIENGAIVSASRR"
BASE COUNT         1013 a         2289 c         2293 g         1095 t
ORIGIN      
        1 cccgccgcgc acttccattg aaaacgtgtg gaaaaggggt caggttactt tgcacaagaa
       61 acaggcaacg cagaaaaggg gtcaggttac ttttcagccc gccgttttgc gacggcaaaa
      121 cgacagaaaa gtaacctgac cccttttctg cgttgcctgt tctttgtgca aagtaacctg
      181 accccttttc gcacgttatc aatggaagtg cgcggcggga gagtcggctt cctcgggaag
      241 ctcggcgtgg accatctccg cctcggggtt ggcgaacagc ggagcgccgc agtcctcgca
      301 gtactcgggc tcgaaggtcc cggagagctt cttcacctcc gtcacgccgc actgcgtgag
      361 ctcggccagc aggtcctcga gcgggccggg gcgcgcgttc tcgtcctcgc gcccatagag
      421 gggccagacg accccgtgcg ccacgtggct gtcgccgtcc accgtgaagc ccacgcggta
      481 ttcgtcgacg cgttcctcgc cgaagcccgc gatcaccgcg cgcagttgcg cggcttccac
      541 gtgcagcgtg ctctcgagga aggccacgcc cgagcggatc gcgtagggcc gcacgcggcg
      601 gtccgcttcc cggcagttca cgtagtaggc gtccgggagc aggcactcga acacgcagcc
      661 cgggagcatc ttggcgatgg tcgggcgggc ctgcgcgatc cattgctcca ggctcgtcgt
      721 ccggccggtg tggccactcg cgtcctcctg ccagcggaag aggggcttgc cgcgcggcgt
      781 ggccacggag gcgatcacga aacgcgagtc ggcaaggagc tgcgccgttt ccggcatgcg
      841 cgcgaagtcg atgcgcggga tctcgccggt gaccgcggcg tcgcccagct tggccgccag
      901 ccgccgcagt tcggagaaat ggcgcggcaa ctggtcgatg gaatagaggt agggcacgag
      961 cgtcacgcgc gtgtcagccg ccagcacgtg cgcctggagc tgcaccttga gcgcctgtgc
     1021 gtcgtcgcgg tcgatgggac cggagggaat ggcatacttg gaccacgcca ccaccggcgc
     1081 cgcgatgagc agcgactccc aggcaaagtc gccctgcatg acggtcacgg attcggagat
     1141 tccctccacg cgctcgatga gcccgtcgta ggcgccggag ttggtctgcc agaggtggtc
     1201 gagggcccca tcgagcggcg cgtcgttgcc gtgctcgaga agcttctcca ggagggcggc
     1261 gatttcgccc tcccagaagc ggtcctcggt gaggctgccg gaggtgtgca ggccattggc
     1321 cagggcgatg agtcgttcgg cgtcccgggt aaggcggggc gcggatttgc ttcgggtgcg
     1381 tgccatggcg ggggagccgg gaaggaaaac accggccgtg gtgacgattc gtcggaggcg
     1441 tattttatca ttgcaccaac gaacgacagg aatcgacgag atgccccatc tgctgaacgt
     1501 gctgggcggc gaattgcgcg cctgctgctt cgatcccctg accgggtact accgcgacgg
     1561 atcctgccgc gaggcggagg acgacaccgg cacgcacctg gtgtgcgcgc gcatgaccct
     1621 cgagttcctc gccttcagcc tcgcgcgggg caacgatctc gtcacgccgc gccccgagat
     1681 gcgcttccgg gggctgaagc ccggcgatcg ctggtgcgtg tgcgcgctgc gctggctgga
     1741 ggcgctcgag gccggggtgg cgccgcccgt ggtgctcgag gcgacgcacg agcgggcgct
     1801 ggacctcgtt cccctggagg aactcaggcg gcacgcgttc gactcgagcc ccagccgcga
     1861 atccccctag cgcccgcgag cccgccgtct caggccgcca ggcgcgtgcg gggatggcag
     1921 acgggcgcgc tgcgatgcag gaaccgcgca agggccttgc gcactgcgca ggccgcgtcg
     1981 tccagcagct tcgaaagttc ctcgcggcgc aattcgtgag cccggcgatc gatctcgaag
     2041 ggagtcggaa ttctcatgat ctgctccttt gtgtaccggg tacatgtacc gggtacattg
     2101 cctttggcgg cgccacatgg cgcccgagca gccaatctaa ttgcgttacc ggtgcgatac
     2161 aatccatgca gatggcacaa gattgatgac attctggaac aagtagcatg ggtataccgt
     2221 cggacatggc cgccttcgtg cgcgcagtgg aacgcggcgg cttctcgtcc gccgcgcgcg
     2281 agctggggct cacgccctcg gccatttcga agctcgtcac gcgcatggaa gggcggctgg
     2341 gcgtgcgcct cctcaatcgc accacgcggc gcctcgccct cacgcccgag ggcgaggcgt
     2401 tcttccaccg cgcccagcgc atcatcgccg acatcgagga ggcggaagac gaggtcgccc
     2461 gcttccgcga gcgcccgcga ggcaagctgc gcctcaatgt cggcaccgcg ttcgggacct
     2521 accagctcgt gcccgccttg ccggagttcc tcgcgcgcta cccggagatc gaggtcgaga
     2581 tcaccatgac cgaccgcatc gtggacctcg tggaggaagg cgcggacgtc gggctgcgca
     2641 ccgggacgct gtcggattcc tccctcgtcg cgcgccgtat ctgcgacatg gagcgcgtga
     2701 tctgcgcctc ccccggctac ctcaggaagc acggcacgcc gcgaagtccc gccgacctct
     2761 cccggcacaa ctgcctggtg ctcgcggccg cgccccagtt ccaccgctgg cctttcgact
     2821 atcccgacgg ggtgaggaat atcgaggtcg cgggcaacgt cacggccaac agcgccgaga
     2881 cgctcgcgca actcgcgatc gtgggcctgg ggcttgtccg gctcgtggac gtgatcgtcg
     2941 ggagcgcgat cgcgaaaggg ctcctgaaac cggtcctcgc cgacgtgcac cacgtggagc
     3001 ccctgccgct gcacgccgtc ttcccgcacg gccgtcatcg ctcgccgcgc gtggcggcgc
     3061 tggttgactt cctggtcgag aagtttgcac acgcgccgtg gcgcgtgcgt gcggggcggc
     3121 gatgacgatc ctcgcgcccg cggtcgcctt cgtcgatctc gagaccaccg gcacctccgc
     3181 caccggtgac cgcgtgacgg aggtcgccat cgtgcgcgtg gtcgagggcg aggtggtcga
     3241 ggagtggtgc acgctcgtcg atcctgagcg ctcgattccc ccggatatcc aggtcctcac
     3301 cggcatcacc aacgagatgg tgaagggcgc gcccaccttc gccgacatcc ggcacgaggt
     3361 gcgcgagcgt ctcgaggggt acctcttcgt tgcgcacaac gcgcgcttcg actacggctt
     3421 cctcaagaac gagttcgcgc gcctggagat gccctttacc gccgacgttc tctgcaccgt
     3481 gcgcctgtcg cgaaggctct atcccgaagc ggtgggccac agcctcgatg ccctcatcgc
     3541 gcgccacggg ctcgccgatc cgctcgattg cgcggacccg gcggcgcgca cgggccggca
     3601 ttcggcgctg ggcgatgcgc gcgccctgcg ccgcttcacg caggtgatcg agcgcgaaaa
     3661 atccggcgac gaaatcgccg cggcggtgaa gcgcctgctc aagatcccga gcctgccgcc
     3721 gcaattgccg ccggatgccc tcgatggaat tcccgaaggc cccggggtct accggttttt
     3781 cggcgtgaac gacctgccgc tctacattgg caagagcgtg aacctgcgcg atcgcgtgcg
     3841 ctcgcacttc acgtccgact accgcaattc caacgatcac cgcctttccg cggagatccg
     3901 ccgcctcgac tggctcgaga ccgcggggga actgggggca ctcctgctgg aatcgcgcct
     3961 ggtgaaggaa gtggcgccgc tgcacaacgc gctgctgcgc cggcggcgaa cctcgtgcgt
     4021 gttgcggctg ccgtcgttgc gcgaggcgcc cgagttcctc gacctggacg agatcgactg
     4081 ggacgcgcgc ggccccgggg cggagtcact ctacgggccg ttttcctccc cgcgggcggc
     4141 gcgcgacctg ctcgaggcca tcgcctccga ggaggggctg tgctggcgcc tgctgggctg
     4201 ggagaagcgc ggcggcccgt gcttcgcgcg gcaggtgaag aagtgccgcg gcgcctgcgt
     4261 gggggaggaa tcgccggaag cgcacaacgt tcgccttgcg acggccctcg aggtgaagcg
     4321 cattcccgac tggccgttcg cgggaccggt ggccatcgtc gagcgcgatc cggcgcgtgg
     4381 catcgaggag gcgcacgtga tccgccagtg gcgccatctc ggcacggcgc gcgacgagga
     4441 cgagctcgcg tcgctggcgg cgatgcggcg gctgccggcg ttcgatccgg acatcctcag
     4501 ggtgctggtg cgctggctcg ccgagcatcc gggaaaggcc cgcctgctgg atggcgagcc
     4561 ggaccgggac atcgccgagt acgcggatgg aagctgctag ccttcgcctg ccgcgagcag
     4621 gtccggcgat tcgttgcgcg ggttgttcac gcgcgtgctg acgacgcgct cctgcgtctg
     4681 cccgtcggcg tagggcctga ggagcagggc ggcctcctcc gctggcgccg agagccaggt
     4741 ctcgtggtcg gcccgggaca gcaccaccgg catgcggtcg tggatcgcag ccagcgcgcg
     4801 gttcgaagtc gtcgtgatga gcgtgtaggt ctccacgggg ggcgcatccg ggttcgcctt
     4861 gtcgtgccag cgttcccaca ggcccgcgaa ggcgaacaca ggctggtccg cgaacgtgat
     4921 cgcgtgcgga atcttgcggc cggccgcgcc ggtccactcg tagaagccgc tggccggcac
     4981 gaggcagcgg cgctgcctga acgcatcgcg gaacgagggc ttttccgcca ccgtctcgct
     5041 cctcgccatg atcatcttgc tgcccgtccc gagatccttc gcccaggggg gcacgagtcc
     5101 ccagcgcagc atcgccacct cgcgcgcgcc ggcttcgctt gtgcgcacga cgggggcctc
     5161 ctgcgtgggc gcgatgttga agcgcttgcg gatgcggtcg tggaccggaa cgttgaacgt
     5221 gtaggcgatg acgttgccgt cggcgtcgag gaaatagcgg ctgcacatgc gcggaagtat
     5281 tgcacaggga ttgccccggc cccggccggc cggtaagctg gcttcagccc atgcaaatgc
     5341 ctctcgcctg cccgagttgc caggcgccga ccgaccagcg gcgcttcgcc cgccaggtcc
     5401 acggcgaggt gatcctcgac atctgctggg actgccacgc cttctggttc gaccagtacg
     5461 aaagcacgca gctggctcct gctgcggtca tcgaactctt ccggctcatc caccagcacc
     5521 gcgccgcgcc cgcccgcccg ctggccgatt cgatggcctg cccgcactgc cgggcgagac
     5581 tcctggtcac gcaggacatc cagcgcacca accggctgac ctaccaccgc tgcccgaacg
     5641 gtcatggccg gctcaccacc tttttccagt tcctgcgcga gaagcagttc atccgttcgc
     5701 tgtcgcagcc ggagatagag cggctgaagg cgaccgtttc ccaggtgcgg tgctcggggt
     5761 gcggcgctcc ggtggacgtg gctcgcgacg ccgcgtgcag cttctgccgc tcgccgctct
     5821 cggtgctgga ttccgaggcc gtcgaaagaa cccttgcggc gctcacgcaa gccgaccgaa
     5881 ggcgcgcacc ccccgactcc gctgcgatgg ccgaggcgtt cgagtcgttg ttgagcacgc
     5941 accagcgggc ggcgcagggc agcccatgga cgcgagatat cgcgcccctg caatccacgc
     6001 cggcgatgat cgacctggtg gtcgagggaa tcggctggct cctcaggacc tgaaacggac
     6061 cttgccgatg aatccaaccc gatttctgcg tgcctgcgcg ttcgtcgccg catgcgccct
     6121 gctgggcgcg tgccagatcg cgccggtgaa acgcgagccg ccgccgctgc ccttcaccgg
     6181 cacgaaatgg atgatggtca ccgagcgcaa gccggctggc gaggcgccct acctcgagtt
     6241 cggcgacggc gcggtgaccg gttcctcggg gtgcaaccgc atcatgggcc gctacatgca
     6301 ggacgcggtc ggcgcgggcg cgatcgtgtt ctcgtcgatc gcgacgacca agcgcatgtg
     6361 cggggacgca tcgatggcgg tcgaggaacg gttgctctcg gtccttcgca cctccaccaa
     6421 cgtcaaggtg acgggcgaca ccctccgcat cgacggctcg gcgggctcgc tcgacttcag
     6481 cgccgcgacg cgctgacgat cgccccgttc tcgatccgga ccctgtcgcc gacgcgccag
     6541 gagggttcgg aattgaagcg gaccaccttg ttggaaccat cctcgaggcg cacgcgcacg
     6601 tcccaggcgg tgaccgcgcc cttgttcttc tcgacctcgt tgccgagaag agcgccgccg
     6661 agtgcgcccg cgacggtggc ggcatccctg
//
LOCUS       DASUEV010000214         7918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1439608, whole genome shotgun sequence.
ACCESSION   DASUEV010000214 DASUEV010000000
VERSION     DASUEV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439608"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFV55_06940"
     CDS             complement(<1..338)
                     /locus_tag="VFV55_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4944074.1"
                     /translation="MIDVIIPAYRGLAQTRRCLESVLGAPVRSAHEVVVIDDASPEPE
                     LSAWLRSLAGGGRFTLLAHARNAGFVASVNEGMRLHADRDVVLLNSDTEVAGDWLDRI
                     AACAAREPRAG"
     gene            568..1350
                     /locus_tag="VFV55_06945"
     CDS             568..1350
                     /locus_tag="VFV55_06945"
                     /inference="COORDINATES: protein motif:HMM:NF010072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HEX4944075.1"
                     /translation="MNSADNVIAGVGLADRPSKSRWPARMDFAQSATGLTLGLFMWGH
                     MFFVSTILISKDAMWTVTKFFEGYFFLGTSYPGMVSVVVAGVLTLVVVHAFLAMRKFP
                     ANYRQYRTFMGHRKLMAHEDTTLWWVQVITGFALFFLAAVHLYQMLMHPGDIGPYGSA
                     DRVWSGRWWPILLVMLFAVELHGGIGLYRLCVKWGWFEGKDVAASRKRLKVAKWVLTV
                     FFLALGLATLAAYMKIGYEHRDRVGERYTPAWVTAEPKEKAR"
     gene            1347..3320
                     /locus_tag="VFV55_06950"
     CDS             1347..3320
                     /locus_tag="VFV55_06950"
                     /inference="COORDINATES: protein motif:HMM:NF006383.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HEX4944076.1"
                     /translation="MKVIYTDVLVIGGGLAGLRLAIGARRRGHDAIILSLVPAKRSHS
                     KAAQGGMQASLGNVTKGQGDNEDVHFEDTVRGSDWGADQEVVRMFVNTSPKAVRELAA
                     WGVPWSRVRKGDRTVVINGQQVTITERDEAHGLLAQRDFGGTKKWRTCYVADATGHAM
                     LTAVADRAIAESIPVHERMEALALIHDGKRCLGAVVRNLVTGEVLAYVSKATAIATGG
                     AGRLYRNTTNAVICEGIGHAIALETGVAALGNMEAVQFHPTAIFPAGILVTEGCRGDG
                     GLLKDASGHRFMPDYEPEKKELASRDVVSRRMEEHIKKGNGAKSRFGEHLWLDITLLG
                     EHHIHHNLREVWDICHYFLGIDPVKDFIAVRPAQHYTMGGVRTKPTGESPTLKGLFAA
                     GEAACWDMHGFNRLGGNSVAETVVAGMIVGEFIADFCDKAENAANVPVGLVQEFVERE
                     KAKLDAILNRRNGEDPSALKGQMQDLMTSNVGIFRTGPELEQAVDGLQKLLVRSRNVG
                     VRSSEPGPNPELVTAYRVQKMIRLALAIAYGAATRTESRGAHFRTDYPRRNDAEWLKR
                     TLATWGSDSDTLPTLNYEALDVKRMELPPGWRGYGAKDYIEHPDTPARAAEVEAIKAS
                     AGNRFAVQQALMPFEHLLPPVLHGRNERIDEPF"
     gene            3331..4074
                     /locus_tag="VFV55_06955"
     CDS             3331..4074
                     /locus_tag="VFV55_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HEX4944077.1"
                     /translation="MADGNAPRKLTFKILRYNPRVPGDKPRLQSYPIEEADGMTLFIA
                     LNEIREKQDASLAFDFVCRAGICGSCAMMIDGRPGLACRTLTKNLGAEVTLAPLPVFE
                     LIADLSVDTGKWMRGMSERLETWVHMKQGEIDLKRIEDRMDPELAEEIYELDRCIECG
                     CCVAGCGTARMREDFVGAVGLNKIARFRLDPRDTRSDEDYYELVGDDSGVFGCMSLLA
                     CHDVCPKQLPLQTQIAFLRRKMVLQGTKG"
     gene            4114..4584
                     /locus_tag="VFV55_06960"
     CDS             4114..4584
                     /locus_tag="VFV55_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX4944078.1"
                     /translation="MKNQRRALVVAMLAGFALTAGAQELTWRNDVAPIVKKGCAECHG
                     ADAPEYGDWMLLSDAKRKKEGPRMDTYTTFMNYVVWPATGAVMRRLDDGASSGGKPGN
                     MYKYLGDSDAERATNLKTIKAWLGDGAWNLARFKARENVPGVTKEQLEKVKAKY"
     gene            4669..6183
                     /locus_tag="VFV55_06965"
     CDS             4669..6183
                     /locus_tag="VFV55_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX4944079.1"
                     /translation="MNAIAVVRRSGLVAGPLLAAACFVWLPESYATGPETQAAFTVAG
                     RATLAMVAWMATWWMTEAVDIEVTALLPVTLFPILGIAPVAKAAAPYASDVIFLFLGG
                     FILGLAIERWGLGRRIAFFTLRLTGTRPGAMTAGFMAATAFLSMWISNTACAAMMVPI
                     AISVIDLVARTSSGEGGGAIAESAQANFATGLLLAVAYSASIGGIATLIGSPPNGIAA
                     RFITQTWGNEVTFLDWMAFGLPFTLLFLPIAWFLLTQVLFPARFAQVAGGREMIAAQY
                     AKLGPLGRGEKVTLAIFAGAAFLWIFGELLRGVTIAGMRPVAGLTDSGVAMLAALALF
                     LAPVDRAKGVRAMDWEHAVKLPWGVLVLFGGGLSLAAAVEANGVSAFIGHATRGLGVL
                     PPLALLLAVVSMTVFLSEVTSNTAQVATMTPVLAAMAPALGLDPKALVVVCALAASSA
                     YMMPVGTPPNAIVFGTGLVRMPQMIRAGLLLNLSGIALITALGWWLVPHFVGGR"
     gene            6404..7915
                     /locus_tag="VFV55_06970"
     CDS             6404..7915
                     /locus_tag="VFV55_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX4944080.1"
                     /translation="MSAVTPGPSPAKTRAEKAPHHEMPWRALLPVVVTVALAVIPAPE
                     GLPQHAWYFFAIFAGVIAGLMVEPLPGGAVGLIGVTLVAILAPYVLYSPAELAKPGFK
                     VVNASLNWALSGFSNATVWLIFGAFMFALGYEKTGLGRRIALQLVKAMGKRTLTLAYA
                     VTIADVILAPFTPSNTARSGGTIYPVIRNLPALYDSKPNDPSARRIGSYLMWVAIAVT
                     CVTSSMFLTALAPNLLALELVKKTAKLDITWTQWFIAFAPVGILLLILVPLIAYWIYP
                     PEVKQGEEVPKWARKELADMGGLSGREITLAVLVMIALALWIFGGAYVNATTAALVVI
                     SLMLVTGVVTWDDITSNKAAWNTLAWFATLVALADGLNRVGFVKWFADGVASSMVGYS
                     PYTAMIVLVLVFFFTHYLFASITAHVTALLPVMLAVGTTIPGLDMAQFSLLLCLTLGI
                     MGMLTPYATGPSPVYYGSGYLPAKDFWVLGAVFGVIFIAAFLLIGLPWMAMIR"
BASE COUNT         1285 a         2663 c         2654 g         1316 t
ORIGIN      
        1 ccggcgcgag gctcgcgagc cgcgcaggcc gcgatccggt ccagccagtc cccggcgacc
       61 tccgtgtcgc tgttgaggag caccacgtcg cgatccgcgt gcaggcgcat gccttcgttc
      121 accgaggcca cgaaacccgc gttccgggca tgcgcaagaa gcgtgaaccg cccgccgccg
      181 gcgagactgc gcaaccaggc ggaaagctcc ggctccgggc tggcgtcatc gatgaccacg
      241 acctcgtgcg ccgaacggac cggggcgccg aggacgcttt ccaggcagcg gcgcgtctgg
      301 gcaagtccgc ggtaggcggg aatgatgacg tcgatcaacg ggagcggcag ggaggaggtt
      361 ggtggctgat tttatcgcgc cgcctcgact tgcacgtcac ggatggcgtt cgagataccc
      421 cccaggcata ccaggaattc ggtgaagacg cgtcttgttg ccttttccgg acaaatgctc
      481 gtttgacccc tgtcaaccga ggcccttcca ttcggatgca aagtgtgcgg gccgaatccc
      541 catcccgggg gggcctggag gagttgtatg aattccgctg acaacgtgat cgccggcgtc
      601 ggcctcgccg accggcccag caagagccgc tggcctgccc gaatggattt cgcccagagc
      661 gccaccgggc tcaccctcgg cctcttcatg tggggccaca tgttcttcgt ctccaccatc
      721 ctcatcagca aggacgcgat gtggacggtg accaagttct tcgagggcta tttcttcctc
      781 ggcacctcct acccgggcat ggtctccgtg gtcgtggcgg gcgtcctcac gctcgtcgtg
      841 gtccacgcct tcctcgccat gcgcaagttc ccggccaatt atcgccagta ccggacgttc
      901 atggggcacc gcaagctcat ggcgcacgag gacacgacgc tgtggtgggt gcaggtgatc
      961 accggattcg cgctcttctt cctcgccgcc gtccacctct accagatgct catgcacccg
     1021 ggcgacatcg gcccctacgg gtcggcggat cgcgtgtggt cgggccggtg gtggcccatc
     1081 ctgctggtga tgctcttcgc ggtcgagctg catggcggca tcggcctgta ccggttgtgc
     1141 gtgaagtggg gctggttcga gggcaaggac gtcgccgcga gcaggaagcg cctgaaggtc
     1201 gccaaatggg tcctcacggt gttcttcctt gcgctgggtc tcgccaccct ggccgcctac
     1261 atgaagatcg gctatgaaca tcgcgaccgt gtcggcgaac gctacacgcc cgcgtgggtg
     1321 acggcggaac cgaaggagaa ggcgcgatga aagtcattta taccgacgtg ctcgtcatcg
     1381 gcggcgggct cgcgggcctg cggctcgcca tcggcgccag gcggcgcggc cacgacgcca
     1441 tcatcctgtc gctggtgccg gccaagcgct cgcactcgaa ggccgcgcag gggggcatgc
     1501 aagcctccct cggcaacgtg accaaggggc agggagacaa cgaggacgtg catttcgagg
     1561 atacggtgcg cggctccgac tggggcgccg accaggaagt cgtgcgcatg ttcgtgaaca
     1621 cgtcgcccaa ggccgtgcgc gagctcgccg cctggggcgt gccgtggagc cgcgtgcgca
     1681 agggcgaccg gacggtggtc atcaacggcc agcaggtcac gatcacggaa cgcgacgagg
     1741 cacacgggct gctggcgcag cgcgatttcg gcgggacgaa gaagtggcgc acgtgttacg
     1801 tggccgacgc cacgggccat gccatgctca ccgcggtggc cgaccgagcg atcgcggaat
     1861 cgatcccggt ccacgagcgc atggaggccc tcgcgctgat ccacgacggg aaacgctgcc
     1921 tgggcgcggt cgtccgcaac ctcgtcaccg gcgaggttct cgcctacgta tcgaaggcca
     1981 cggccatcgc cacgggaggc gcgggccgcc tgtaccggaa tacgaccaac gcggtcatct
     2041 gcgagggcat cggccacgcc attgccctcg aaacgggcgt ggcggcgctg ggcaacatgg
     2101 aggcggtgca gttccacccg accgccatct tccccgcagg cattctcgtg accgagggct
     2161 gccgcggcga cgggggactc ctcaaggacg cgagcggaca ccgcttcatg cccgactacg
     2221 agccggagaa gaaggagctc gcctcgcgcg acgtggtctc gcggcgcatg gaggagcaca
     2281 tcaagaaggg caacggggcg aagtcgcgct tcggcgagca cctctggctc gacatcacgc
     2341 tgctcggcga gcaccacatc caccacaacc tgcgcgaagt ctgggacatc tgccactact
     2401 tcctcggcat cgacccggtc aaggatttca tcgccgtgcg gcccgcgcag cactacacga
     2461 tgggcggcgt gcgcacgaag cccaccgggg agagcccgac gctcaagggg cttttcgccg
     2521 ccggcgaggc tgcgtgctgg gacatgcacg gcttcaaccg cctgggtggc aactccgtgg
     2581 ccgaaaccgt ggtcgcgggc atgatcgtgg gcgaattcat cgccgatttc tgcgacaagg
     2641 cggagaacgc ggccaacgtg cccgtcgggc tggtgcagga gttcgtcgag cgcgagaagg
     2701 cgaagctcga cgcgatcctg aaccgccgca acggcgagga cccctcggcg ctcaaggggc
     2761 agatgcagga tctcatgacc tccaacgtgg gcatcttccg caccgggccg gagctcgagc
     2821 aggcggtcga cgggctgcag aagctcctcg tgcgcagccg caacgtgggc gtgcgctcgt
     2881 cggagccggg cccgaatccc gagctcgtca ccgcgtaccg ggtacagaag atgatccggc
     2941 tggcgcttgc gatcgcctac ggtgccgcga cgcgaacgga aagccgcggc gcgcacttcc
     3001 gcaccgacta cccgcggcgc aacgacgccg aatggctcaa gcgcacgctg gcgacttggg
     3061 ggagcgattc ggacacgctg ccgacgctga actacgaggc gctcgacgtg aagcgcatgg
     3121 agctgccgcc gggctggcgc ggctacggcg ccaaggacta catcgaacat ccggatacgc
     3181 ccgcgcgcgc cgccgaagtc gaggccatca aggcatccgc ggggaaccgc ttcgcggtcc
     3241 agcaggccct gatgccattc gaacacctgc tcccgccggt cctgcatggc cgcaacgagc
     3301 gcatcgacga accgttctga ggagcagacg atggccgacg gcaacgcccc acgcaagctc
     3361 accttcaaga tcctgcgcta caacccgagg gtgcccggcg acaagccgcg gctgcagagc
     3421 tacccgatcg aggaagccga cgggatgacg ctcttcatcg ccctgaacga gatccgcgaa
     3481 aagcaggacg cctcgctagc cttcgatttc gtgtgccgcg cgggcatctg cggaagctgc
     3541 gcgatgatga tcgacggacg ccccgggctc gcgtgccgca cgctcaccaa gaacctcggc
     3601 gccgaggtca ccctcgcgcc cctgccggtc ttcgagctca tcgccgacct ctcggtggac
     3661 acgggcaagt ggatgcgtgg catgagcgag cgcctcgaga cctgggtgca catgaagcag
     3721 ggcgagatcg acctgaagcg gatcgaggat cgcatggatc ccgaactcgc cgaggagatc
     3781 tacgaactcg accgctgcat cgagtgcgga tgctgcgtgg ccggctgcgg cacggcgcgc
     3841 atgcgcgagg atttcgtggg ggccgtcggc ctcaacaaga tcgcgcgttt ccggctggac
     3901 ccgcgcgaca cgcgctccga cgaggattac tacgagctgg tcggcgacga ttccggcgtc
     3961 ttcggctgca tgtcgctgct ggcttgccac gacgtgtgcc cgaagcagct tccgctgcag
     4021 acgcagatcg cattcctgcg caggaagatg gtcctgcagg gaacgaaggg ctgagcatcc
     4081 acaattcaaa ccaaggcaag gggaggacaa gaaatgaaga accagcgaag ggcactcgtg
     4141 gtcgccatgc tcgcgggctt cgcccttacg gcgggcgccc aggagctcac ctggcgaaat
     4201 gacgtcgccc ccatcgtcaa gaagggctgc gccgagtgtc atggcgcgga tgccccggag
     4261 tatggcgact ggatgctgct ttccgacgcc aagcgcaaga aggagggccc gcgaatggat
     4321 acctacacca cgttcatgaa ctacgtggtg tggcccgcga cgggtgccgt gatgcgcagg
     4381 ctcgacgacg gcgcctcgtc cggcggcaag cccggcaaca tgtacaagta cctcggcgat
     4441 tccgacgcgg agcgcgcgac gaacctgaag acgataaagg cctggctcgg tgacggtgcg
     4501 tggaacctcg cccggttcaa ggcgcgcgag aacgtcccgg gcgtgaccaa ggaacagctc
     4561 gagaaggtca aggcgaagta ctagccgaaa tatccggcgg cattttcgct gcccgccggc
     4621 ttcgcgcgtg acggccgccg cactgcggtg gctgcctgaa ccgacgccat gaacgcgatc
     4681 gccgtcgtac gccgctcggg cctcgtggcg gggcctctcc tcgccgctgc ctgcttcgtg
     4741 tggcttccgg aatcctatgc gacgggcccg gagacgcagg ccgccttcac cgtggccggc
     4801 cgcgcgacgc tcgcgatggt cgcctggatg gcgacctggt ggatgacgga ggccgtcgac
     4861 atcgaggtca cggcgctgct cccggtcacg ctctttccga tcctgggcat cgcgccggtt
     4921 gcgaaggcgg ccgcacccta cgcgtcggac gtgatcttcc ttttcctggg cgggttcatc
     4981 ctcggcctcg ccatcgagcg ctgggggctc ggaaggcgca tcgcgttctt cacgctgcgc
     5041 ctcacgggca cgcgcccggg ggcgatgacg gccggcttca tggcggcgac tgcgttcctc
     5101 agcatgtgga tctcgaacac cgcctgcgcg gcgatgatgg tgcccatcgc gatcagcgtg
     5161 atcgatctgg tggcgcgaac gagctccggc gagggcggcg gtgcgatcgc cgaatccgcc
     5221 caagccaatt tcgccaccgg cctgctgctc gccgtggcct actccgcatc catcggcggc
     5281 atcgcgactc tcatcggttc cccgccgaac ggcatcgcgg cgcgcttcat cacgcagacc
     5341 tggggcaacg aggtcacctt cctcgactgg atggccttcg gcctgccgtt cacgctcctg
     5401 ttcctgccca tcgcctggtt cctgctcacg caggtgctgt ttccggcgcg gttcgcgcag
     5461 gtggcgggag gccgcgagat gatcgcggcg caatacgcga agctgggtcc cctggggcgc
     5521 ggcgaaaagg tcacgctggc gatcttcgcg ggcgcagcct tcctgtggat cttcggcgag
     5581 ttgctgcgcg gcgtaaccat cgccggcatg cgcccggttg ccgggctcac ggacagtggc
     5641 gtggccatgc tcgcggcgct cgccttgttc cttgccccgg tggaccgcgc gaaaggcgtg
     5701 cgcgcgatgg actgggagca cgcggtgaag ctgccgtggg gcgtgctggt gctcttcggc
     5761 gggggcctga gcctcgccgc ggcggtcgag gcgaacggcg tgtcggcatt catcggccat
     5821 gccacgcgcg ggctgggcgt gctgccgccg ctcgcgctcc ttctggccgt ggtgtcgatg
     5881 accgtgttcc tctccgaagt cacctccaat accgcgcagg tcgccacgat gacgcccgtt
     5941 ctcgcggcca tggcgcccgc cctcggcctc gatccgaagg ccctcgtcgt ggtgtgcgcg
     6001 ctcgcggcga gcagcgccta catgatgccc gtgggcacgc cgccgaacgc gatcgtgttc
     6061 ggcacggggc tggtgcgcat gccgcagatg atcagggcgg ggctgctgct caacctttcg
     6121 ggcatcgcgc tgatcaccgc cctgggctgg tggctcgtgc cgcacttcgt cggcggccgc
     6181 tgaattccac ggcgcccgtc ggcgcctgtc cgttgcacca aagtcccatg cggcttgccg
     6241 cgcgatgcgc gcgatgcggc gcgcatcggt tgcaccaaag accaatggaa tgcgcaatgg
     6301 cgctccggcg cttgagtagg atcaaaaaaa atcgcggatg cggcgtgaga atcctttgcg
     6361 aaaggtcgcg gacttcgcga tcgcatgcga aaggatgtag atcatgtctg ctgtcacccc
     6421 gggcccatcg cccgcgaaaa cccgcgccga aaaggctccc catcacgaga tgccctggcg
     6481 tgcgctgcta cccgtcgtcg tcaccgtggc gctcgcggtc attcccgcgc ccgagggcct
     6541 gccgcagcac gcgtggtact tcttcgccat cttcgccggc gtcatcgccg gcctgatggt
     6601 cgagccgctt cccggcggtg ccgtcgggct catcggcgtg acgctggtcg ccatcctggc
     6661 cccgtacgtg ctctactcgc ccgccgagct cgcgaagccg ggcttcaagg tggtgaacgc
     6721 atcgctcaac tgggcactct ccggcttctc caacgccacg gtgtggctca tcttcggcgc
     6781 gttcatgttc gcgctgggct acgagaagac gggcctcggc cgcaggatcg cgctgcagct
     6841 cgtgaaggcg atgggcaagc gcaccctcac gctggcctac gcggtgacga ttgccgacgt
     6901 gatcctcgcg cccttcacgc cgtccaacac ggcgaggagc ggcggcacga tctacccggt
     6961 gatacgcaac ctgcccgcgc tctacgattc gaagcccaac gacccgtccg cccgccgcat
     7021 cggttcctac ctgatgtggg tggcgatcgc ggtgacctgc gtgacgagct cgatgttcct
     7081 caccgcgctg gccccgaacc tcctcgccct ggaactggtc aagaagacgg cgaagctcga
     7141 catcacctgg acgcagtggt tcatcgcgtt cgcgccggtc ggaatcctgc tcctcatcct
     7201 cgtcccgctg atcgcctact ggatctaccc gcccgaagtg aaacagggcg aggaagttcc
     7261 gaaatgggcc aggaaggaac tcgccgacat gggtgggctc tccggccgcg agatcacgct
     7321 ggcggtcctg gtgatgatcg cgctggcgct gtggatcttc ggcggggcgt acgtgaacgc
     7381 gacgaccgcc gccctcgtgg tcatctcgct catgctcgtc acgggcgtgg tgacctggga
     7441 tgacatcaca tccaacaagg cggcatggaa cacgctcgcg tggttcgcga ccctcgtcgc
     7501 gctggccgac ggcctgaaca gggtcggctt cgtgaagtgg ttcgccgacg gcgtggcctc
     7561 gagcatggtc ggctactcgc cctacaccgc gatgatcgtg ctggtgctgg tgttcttctt
     7621 cacgcactac ctgttcgcga gcatcaccgc gcacgtcacg gcgctccttc cggtgatgct
     7681 ggcggtgggc acgacgattc ccggcctcga catggcgcag ttctcgctgc tcctgtgcct
     7741 cacgctcggg atcatgggca tgctcacgcc gtacgcaacg ggcccgagcc cggtgtacta
     7801 cggctccggc tacctgcccg cgaaggactt ctgggtactg ggcgcggtct tcggcgtcat
     7861 cttcatcgcg gccttcctgc tcatcgggct gccgtggatg gcgatgatcc gctgaccc
//
LOCUS       DASUEV010000215         6470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_71781, whole genome shotgun sequence.
ACCESSION   DASUEV010000215 DASUEV010000000
VERSION     DASUEV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71781"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFV55_06975"
     CDS             complement(<1..343)
                     /locus_tag="VFV55_06975"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4944081.1"
                     /translation="MEERLNALGRVSAQEGEQVGNSREGLLAEVRDGVATVTLDRPAA
                     LNALNLAMLEGLAAWLDGWERDDRVRLVVLRGAGGKAFCAGGDIRELREMFVAGSGVH
                     RHFFEVEYALDH"
     gene            complement(328..1095)
                     /locus_tag="VFV55_06980"
     CDS             complement(328..1095)
                     /locus_tag="VFV55_06980"
                     /inference="COORDINATES: protein motif:HMM:NF006566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX4944082.1"
                     /translation="MITHRIEGHTALVTIDNPPANTWTPEDLQALEKLVGELNADRSV
                     YAAVITGAGEKFFSAGADLKKFASDKVHARHFIACFGSAFEALMNARFVTIAAINGYA
                     MGGGLECCLACDLRIAESHAQMALPEPGVGLLPAGCGTQNLPGLVGEGWAKRMILTNE
                     RVDAATALRIGLVEEVADKGQGLAKAMALATRVASLSPRAVEYCKGLIHNARNAIPRR
                     AGLALERERFMDLFDHSDQLEGVNAFLEKRAPAWRND"
     gene            complement(1117..2001)
                     /gene="mmsB"
                     /locus_tag="VFV55_06985"
     CDS             complement(1117..2001)
                     /gene="mmsB"
                     /locus_tag="VFV55_06985"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX4944083.1"
                     /translation="MKIGFIGLGNMGNPMCRNLLKNGHTVKAYDVVPAHLAKAVSQGA
                     SRADSVADCVSGAEVVITMLPSSPHVRSVYCGESGVIAHARAGTLLIDSSTIDPLTAR
                     EVAFDALARGLAMVDAPVSGGTGGAESGTLTFMVGGNAREFDSAKPLLACMGKNIVHC
                     GASGNGQVAKICNNMMLAIEMIATSEAMTLATALGMDAKVFAGIVNSSSGRCWSSDTY
                     NPYPGVLENVPASRGYAGGFGADLMLKDLTLVTDAARTAKVPVLLGALAHQLYQKHSA
                     DGHGGKDFSSVILQYLKQ"
     gene            complement(2007..3533)
                     /locus_tag="VFV55_06990"
     CDS             complement(2007..3533)
                     /locus_tag="VFV55_06990"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX4944084.1"
                     /translation="MAANLQAVAQAVPTVKLLINGQFVDSKTTEWKDVVNPASQQVLA
                     RVPFATPAELDAAVAAAATAYKTWKNTPVGARMRIMLKLQELIRRDMKELATCLTHEQ
                     GKTLADAEGDVFRGLEVVEHACSVGTIQMGEHLEQVANGVDVYAIRQPIGVCVGITPF
                     NFPAMIPLWMFPMAIVCGNTFVLKPSEQDPMTPMLLAELATEAGLPPGVLNIVHGGKV
                     AVDTLCTHADVRAVSFVGSTHVGHHVYNLASTHGKRAQCMMGAKNHAVVLPDAAKDQA
                     LNAVVGAGFGAAGQRCMAISVAVLVGKANEWIPDIVAKAKSLKVTSGMEAGADLGPLI
                     SKAALARVMALTDTGEKEGAKLELDGRGLKVPGYADGNFFGPTIFSGVKPSMTIYKEE
                     IFGPVLCLVSVDTLDEAIAFINANPFGNGTGIFTQSGAAARKFQYEVDVGQVGINVPI
                     PVPVPYFSFTGSRGSKLGDLGPYGKQVMQFYTQAKTVTARWFDDAVTTGAVNTTISLK
                     "
     gene            3730..4437
                     /locus_tag="VFV55_06995"
     CDS             3730..4437
                     /locus_tag="VFV55_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX4944085.1"
                     /translation="MFPWNHDRPPSPLAALRDVSLSKVVREDILGLILRGDIAPGSRI
                     NEPDVAERLGVSRVPVREALRELESTGLVVSRKNVGVFVREFAPREVADLYELRAVLD
                     GHAGTRAAALAEAPRRALSRILDAATAAMRKAARRGDVPAYYAENLRFHWAIVEAAGN
                     EKLSDTYRGIVQQLHLWRLRNLAQPVGMAASVAEHEAIAKAIRDADPAGAGRLLADHV
                     GAARQRLEQHLSEEKTP"
     gene            4455..5405
                     /locus_tag="VFV55_07000"
     CDS             4455..5405
                     /locus_tag="VFV55_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944086.1"
                     /translation="MSAVLLAAAALLPGLAPAQDYPTKTVKIIAPVQPGGGVDLVGRT
                     VAEQLGKALGQTFMVENISGGGGVIASQAVARAAPDGYTLMVGYVATHGTVPAVRKVP
                     YDAVKDFTPVAMVAGTPNVLVAGATVPVTDVKSLIEYAKKNKVSYGSAGLGSLTHLAM
                     EQFRTAAGFEAVHAAYRGIGPAITDILGGQTQLMMPGLAAAVPHIRAGKMKPLAVTGL
                     KRHPLLPEVPTFEELGYKGFDGVQWYGIVGPANMPPAVTRKLNDEINKALATPALQQR
                     LAGEALDPMPMTPEQFGSFMQADIAHWAKLARERNISIEN"
     gene            5408..>6470
                     /locus_tag="VFV55_07005"
     CDS             5408..>6470
                     /locus_tag="VFV55_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX4944087.1"
                     /translation="MARNTQVPADHNAPPVTRALARFVATHPSRGWNDAVDHEAHRTF
                     LNWAGCAVGGARHEAAQAALDAAAMLQPAPQSTVLGRSEKVDMASAALINGITSHVFD
                     FDDTHLKTIIHPGGPVASAVFALAETTGASGREVIDALVLGIDVSCRLGNVMYPDHYD
                     RGWHITGSTGMLGAAAGCSRLLGLDEERTAMALGIAASQPIGMREQFGTMTKPFHPGG
                     AARAGLFSALLAKHGFTASPRASEAPRGYVQVVSTKCDWNEATDELGERFEISFNTYK
                     PFACGIVIHPAIDACVQLRARGVKAQDVERIELKVHSLVLELTGKKEPQDGLQGKFSI
                     YHGCAAGLLFGRAGEAEYAD"
BASE COUNT         1014 a         2186 c         2235 g         1035 t
ORIGIN      
        1 gatgatcgag tgcgtactcg acttcgaaga agtggcggtg cacgccggag ccggcgacga
       61 acatctcgcg aagctcgcga atgtcgccgc ccgcgcagaa ggccttgccg cccgcaccgc
      121 gcagcacgac aagccggacg cggtcgtccc tttcccagcc gtcgagccag gcggcgagcc
      181 cctcgagcat ggccaggttg agcgcattga gggcggcggg ccggtccagg gtgacggtgg
      241 ccaccccgtc gcgcacctcg gcgagaagtc cctcgcggga atttccgact tgctccccct
      301 cttgcgcgga cacccggccg agggcgttca gtcgttcctc catgccggcg cgcgcttctc
      361 gaggaaggcg ttcacgccct cgagctggtc ggaatggtcg aagaggtcca tgaagcgctc
      421 gcgttcgagc gcgaggccgg cgcggcgcgg gatggcgttg cgcgcattgt ggatgaggcc
      481 cttgcaatac tcgacggcgc ggggcgagag cgaggcaacg cgcgtggcga gcgccatcgc
      541 cttcgcgagc ccttgtccct tgtcggccac ttcctcgacg agcccgatgc gcagcgccgt
      601 ggccgcgtcg acgcgctcgt tggtcaggat catgcgcttg gcccagccct ctcccacgag
      661 cccgggcagg ttctgcgtgc cgcacccggc cggcagcagg cccacgccgg gttcgggcag
      721 cgccatctgg gcgtgcgact ccgcgatgcg caggtcgcag gcgaggcagc actccagccc
      781 gccgcccatc gcgtagccgt tgatcgcggc gatggtgacg aagcgcgcgt tcatgagcgc
      841 ctcgaaggcc gagccgaagc aggcgatgaa gtggcgcgca tggaccttgt cgctggcgaa
      901 cttcttcagg tcggcaccgg cgctgaagaa tttttcgccc gcgcccgtga tgaccgcggc
      961 gtacacgctg cggtcggcat tgagctcgcc cacgagcttc tccagggcct gcaggtcctc
     1021 gggcgtccac gtgttggccg gggggttgtc gatggtgacg agggcggtgt gtccctcgat
     1081 gcggtgggtg atcatttgtc ggggaggtct cgcgggctat tgcttgaggt attggaggat
     1141 gacgctggaa aagtccttgc cgccgtgccc gtccgcggaa tgcttctggt agagctggtg
     1201 cgcgagggcg cccaggagca cgggaacctt ggccgtcctg gcggcatcgg tgacgagcgt
     1261 gaggtccttc agcatcaggt ccgccccgaa gccgcccgca tagccgcgcg acgccggcac
     1321 gttctcgagg acgccggggt agggattgta ggtgtcggaa ctccagcacc ggccgctcga
     1381 gctgttcacg atgccggcga acaccttcgc gtccatgccc agcgccgtgg cgagcgtcat
     1441 cgcttccgag gttgcgatca tctcgatcgc gagcatcatg ttgttgcaga tcttggccac
     1501 ctgcccgttg ccgctcgccc cgcagtggac gatgttcttc cccatgcagg cgaggagggg
     1561 cttcgccgaa tcgaattccc gggcattgcc gccgaccatg aaggtgagcg tgccggattc
     1621 cgcgccgccg gtgccgccgg agaccggcgc gtcgaccatg gctagccccc tggccaacgc
     1681 gtcgaaggcc acctcgcggg cggtgagcgg gtcgatggtg gaactgtcga tgaggagcgt
     1741 gcctgccctg gcatgcgcga tgacgcccga ctcgccgcag taaacgctgc gcacgtgcgg
     1801 ggaggagggc agcatcgtga tgactacctc ggcgccggag acgcagtcgg ctaccgaatc
     1861 ggcccgggaa gcaccctgcg acaccgcctt cgcgaggtgg gcgggaacca cgtcgtaggc
     1921 cttgacggtg tgcccgttct tgagcaggtt gcggcacatg gggttgccca tgttgcccag
     1981 gccgatgaag ccgatcttca tcgccgctac ttcaggctga tggtggtatt gaccgcgccg
     2041 gtggtgaccg cgtcgtcgaa ccagcgcgcc gtcaccgtct tcgcctgagt atagaactgc
     2101 atcacctgct tgccgtaggg ccccaggtcg cccagcttgg agccgcgcga gccggtgaag
     2161 ctgaagtagg gcaccggcac cgggatcggc acgttgatgc ccacctggcc cacgtcgacc
     2221 tcgtactgga acttgcgcgc cgccgccccg gactgcgtga agatgccggt gccgttgccg
     2281 aaggggttgg cgttgatgaa ggcgatcgct tcgtccagcg tatccaccga gacgaggcac
     2341 agcacggggc cgaagatctc ctccttgtag atggtcatcg acggcttcac gccggagaag
     2401 atcgtcgggc cgaagaagtt gccgtcggca tagccgggca ccttgaggcc gcggccgtcg
     2461 agctcgagct tggcgccttc cttctcgccc gtgtccgtca gggccatgac gcgcgcgagc
     2521 gcggccttcg agatgagggg acccaggtcc gcgcccgcct ccatgccgga ggtcaccttg
     2581 aggctcttcg ccttggccac gatgtcggga atccactcgt tggccttgcc cacgagcacc
     2641 gcgacgctga tcgccatgca gcgctggccc gcggcgccga agcccgcgcc gaccacggca
     2701 ttgagcgcct ggtccttcgc ggcgtccggc agcaccaccg catggttctt ggcgcccatc
     2761 atgcattgcg cgcgctttcc gtgcgtcgac gcgaggttgt agacgtggtg gccgacgtgg
     2821 gtcgagccca cgaaggacac ggcccgcacg tcggcgtgcg tgcatagcgt gtccaccgcg
     2881 accttgccgc cgtgcacgat gttcaatacg cccgggggca ggcccgcttc ggttgcgagt
     2941 tccgccagca gcatgggcgt catcgggtcc tgctcggacg gcttgaggac gaaggtgttg
     3001 ccgcagacga tcgccatggg gaacatccac agcgggatca tggccgggaa attgaagggc
     3061 gtgatgccga cgcacacgcc gatgggctgg cggatcgcgt agacgtcgac accgttggcc
     3121 acctgctcga ggtgctcgcc catctggatg gtgcccacgg agcacgcgtg ttccacgacc
     3181 tcgaggccgc ggaagacgtc gccctcggca tcggccaggg tcttgccctg ctcgtgggtc
     3241 aggcaggtgg cgagttcctt catgtcgcgg cggatgagct cctggagctt gagcatgatg
     3301 cgcatgcgcg cgcccaccgg ggtgttcttc cacgtcttgt acgccgtggc ggctgccgcg
     3361 acggcggcgt cgagctcggc gggcgtggcg aagggcacgc gcgcaagcac ctgctgcgag
     3421 gcggggttga ccacgtcctt ccactcggtg gtcttcgaat cgacgaactg gccattgatc
     3481 agcagcttga ccgtggggac ggcctgcgcg acggcctgga ggttggcagc catggaattc
     3541 tcctcggaat cgcccgcggt tgggtgggcg ggcggatggg cgtttcgggt tgtgcctgtg
     3601 cttccgggat tataagtctt cgtggcgatc cgtctgccgc cgcggcgcag gatgacggct
     3661 gctactcgcg gtatcgtata cgatatactc ggtgtcagac tgcacccggt gtcagactcc
     3721 accaccgcca tgttcccctg gaaccacgat cgtcctccgt cgccgctcgc cgcactgcgc
     3781 gatgtctcgc tctcgaaggt ggtgcgcgag gacatcctcg gcctgatcct tcgcggggac
     3841 atcgcgccgg gcagccggat caacgagccc gacgtggccg agcgcctcgg cgtatcgcgc
     3901 gtcccggtgc gggaggcgct gcgcgagctc gagtccacgg gcctcgtcgt gtcgcgcaag
     3961 aacgtcggcg tgttcgtgcg cgagttcgcg ccccgggagg tggcggatct ctacgaactg
     4021 cgcgccgtgc tcgacggcca tgcgggcacg agggctgctg ccctggccga ggcgccgcga
     4081 cgggcgctct cgaggatcct ggacgccgcc acggccgcca tgcgcaaggc ggcgcgccgc
     4141 ggcgacgtgc ccgcctacta tgccgagaac ctgcgcttcc actgggcgat cgtggaggcg
     4201 gccggaaacg aaaagctttc cgacacctac cgcggcatcg tgcagcaact gcacctctgg
     4261 cggctcagga acctggccca gcccgtgggc atggccgcct cggtcgcgga gcacgaggcg
     4321 atcgcgaagg ccattcgcga cgccgatccg gcaggcgccg ggcggctgct cgccgaccac
     4381 gtgggtgccg cccgccaacg actcgaacaa catctctcgg aggagaaaac gccatgaatg
     4441 ctcgccatca agcaatgtcc gcagtccttc tggccgccgc cgcgctgctg ccgggacttg
     4501 cgccagcgca ggattacccc acgaagaccg tgaagatcat cgcccccgtc cagcccggcg
     4561 gcggcgtgga cctcgtcggc cgcacggtcg ccgagcagct cggcaaggcg ctcggccaga
     4621 cgttcatggt tgaaaacatt tccggcggcg gcggtgtcat cgcctcgcag gccgtggccc
     4681 gggcggcccc ggacggctac acgctgatgg tgggctacgt ggccacgcac ggcaccgtgc
     4741 cggccgtccg caaggtgccg tacgacgcgg tgaaggactt cacccccgtt gcgatggtcg
     4801 ccggcacgcc gaacgtgctc gtggccggcg ccaccgttcc cgtcacggac gtcaagtcgc
     4861 tcatcgagta cgcgaagaag aacaaggtga gctacggctc ggcgggcctg ggctcgctca
     4921 cccaccttgc catggagcag ttccggaccg ccgccgggtt cgaagcggtg cacgccgcct
     4981 atcgcggcat aggacccgca atcaccgaca tcctgggcgg ccagacgcag ttgatgatgc
     5041 cgggcctcgc agccgccgtg ccgcacatcc gcgccggcaa gatgaagccc ctcgcggtta
     5101 ccggactcaa gcgccatccg ctcctgccgg aggtgcccac cttcgaggaa ctgggctaca
     5161 agggcttcga cggcgtgcag tggtatggca tcgtgggacc ggccaacatg ccgcccgcgg
     5221 tgacgaggaa gctgaacgac gagatcaaca aggcgctcgc caccccggcc ctgcagcaac
     5281 ggctcgccgg cgaggcgctc gaccccatgc cgatgacgcc cgagcagttc ggaagtttca
     5341 tgcaggccga catcgcgcac tgggcgaagc tcgcgcgcga acggaacatc tcgatcgaga
     5401 actgatcgtg gcgcgcaata cgcaggttcc cgcagaccac aacgccccgc ccgtcacgcg
     5461 tgccctcgcg cgattcgtcg cgacgcaccc gtcgcgcggg tggaacgacg ccgtcgatca
     5521 cgaagcacac cgcacctttc tcaactgggc gggctgcgca gtgggcggcg cgcgccacga
     5581 ggcggcgcag gcggcgctgg acgccgccgc catgctgcaa ccggccccgc aatcgacggt
     5641 gctcggccgc agcgaaaagg tggacatggc gagcgccgcg ctcatcaacg gcatcacctc
     5701 ccatgtcttc gatttcgacg acacgcacct gaagaccatc atccatcccg gcggccccgt
     5761 ggcttccgcg gtcttcgcgc tggcggaaac gaccggcgcg agcggccggg aggtgatcga
     5821 cgcgctggtg ctgggcatcg acgtctcgtg ccggcttggc aacgtcatgt acccggacca
     5881 ctacgaccgc ggctggcaca tcaccggctc gaccgggatg ctcggcgcag ccgcgggctg
     5941 ctcgcgcctc ctgggactcg acgaggaacg gacggcgatg gcgctcggca tcgccgcgtc
     6001 gcagccgata gggatgcgcg agcagttcgg caccatgacc aagcccttcc atcccggcgg
     6061 cgcagcgcgc gcggggctct tctcggcgct gctcgcaaag cacggcttca ccgcgagccc
     6121 gagagccagc gaggcgccgc gcggctacgt gcaggtcgtc tccaccaagt gcgactggaa
     6181 cgaggccacg gacgaactgg gcgagcgctt cgagatctcc ttcaacacct acaagccgtt
     6241 cgcctgcggg atcgtgatcc accccgcaat cgacgcctgc gtgcaactgc gcgcgagggg
     6301 cgtgaaggcg caggacgtcg agcgcatcga gttgaaggtg cattcgctgg tgctggagct
     6361 caccggcaag aaggaaccgc aggacggact gcagggcaag ttcagcatct atcacggatg
     6421 cgccgcgggg ctccttttcg gccgcgccgg cgaggcggag tatgccgacg
//
LOCUS       DASUEV010000216         6669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1195162, whole genome shotgun sequence.
ACCESSION   DASUEV010000216 DASUEV010000000
VERSION     DASUEV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195162"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1081
                     /gene="mtnA"
                     /locus_tag="VFV55_07010"
     CDS             32..1081
                     /gene="mtnA"
                     /locus_tag="VFV55_07010"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX4944088.1"
                     /translation="MHIIDQTRLPHAFETRALDSPEAVAEAIETMRVRGAPLIGVTAA
                     FGLALAMSRDSSDESLAKTAARLAATRPTAVNLAWAIERMREALDGLAPHERPAAAWF
                     EAQAIADQDAALNEAIGRHGLVLIEREHAARSRTVNILTHCNAGWIATVDWGTALAPI
                     YMAHDKGIPVHVWVDETRPRSQGLLTAWELAGHGVPHTLVADNAGGHLMQHGKVDLVL
                     VGADRVTRRGDVANKIGTYLKALAARDNDVPFYVAVPTPTIDWETEDALAGIPIEERS
                     GEEMRVVRGLDGSGRLASVAIAQDTTPVANPAFDVTPAALVTGIITEKGVFAAKEAAL
                     LALYPSERRSKAFLT"
     gene            1078..1719
                     /locus_tag="VFV55_07015"
     CDS             1078..1719
                     /locus_tag="VFV55_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX4944089.1"
                     /translation="MNDADLRERIVATALRMNALGINQGSSGNVSARVEGGFLVTPSG
                     LPYEDLQDADIVHVGMGGHATGHREPSSEWRFHRDIYATRPEAGAVVHTHSAFATTLA
                     CLGRPIPAFHYEVAFAGGNDIRCAPYRTFGTQELSDAALAALEDRRACLLAHHGIIAF
                     GSDLDDALRLADKVEALARLYWQAIQVDEPTVLDDAEMARIVERFRHYGPRSG"
     gene            1801..3624
                     /locus_tag="VFV55_07020"
     CDS             1801..3624
                     /locus_tag="VFV55_07020"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX4944090.1"
                     /translation="MHAQSAPVPGAAHTRNARLLKWVADVAALTQPDAIYWCDGTEEE
                     YDRLCAQLVEAGTFRRLNDKLRPNSYLAWSDPSDVARVEDRTFICSKQKDDAGPTNNW
                     VEPAEMRATLKGLFSGCMRGRTMYVVPFSMGPIGSHIAHIGVEISDSAYVAVNMKLMT
                     RMGASVLATLGDHGNFVPCVHSVGVPLEPGDNDVPWPCNREHKYIVQFPEDREIWSYG
                     SGYGGNALLGKKCFALRIASVMGREQGWLAEHMLILGVESPEGVKHYVTAAFPSACGK
                     TNFAMLIPPAVFKGWKVTTIGDDIAWIKPGEDGRLHAINPEAGFFGVAPGTGYDTNPN
                     CMEAVKTNTIFTNVALTPEGDVWWEGMTKEVPAKLTDWTGKDWTPGCGRPAAHPNSRF
                     TVAATQSPSIDPAWDDPNGVPISAFVFGGRRSTTVPLVTEAATWDEGVYMAATLGSET
                     TAAITGKVGVVRRDPFAMLAFCGYNISDYFAHWIETGRKIALPPKIYLVNWFRKDDEG
                     KYVWPGYGENMRVLKWIVERVEGLADAERTAVGYVPRHKDIDWRGMDSFGADKYLAIT
                     AIDKAMWKQEMPLHREFFDKLGERLPAVMRQRFQALAGTLG"
     gene            3671..4096
                     /locus_tag="VFV55_07025"
     CDS             3671..4096
                     /locus_tag="VFV55_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX4944091.1"
                     /translation="MNATLAEAGRFLRYGIAGLVATATHYAVMVALVWTFAGHEVLAS
                     SMGFIAGAFVKYPLNYWLVFASRQRHRVAVPRFVIGLALAFALNAVLLALLLRSLDAH
                     YMVSQVLTTGVVIFANYVVARLWIFRHRPGTSGPGSDRR"
     gene            complement(3996..4832)
                     /locus_tag="VFV55_07030"
     CDS             complement(3996..4832)
                     /locus_tag="VFV55_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX4944092.1"
                     /translation="MINGKKVVVVMPAYRAAATVEKTWRALPHAIVDHVLLVDDASGD
                     ETAAVARALGIDVHMHSENRGYGGNQKTCYSAALAHGADIVVMVHPDYQYEPRLVTAM
                     AAMIESGVYDVVLGSRILGGAALSGGMPLWKYGANRALTAFQNLVTGAKLSEYHSGYR
                     AFSRQALQSLPLLANSDDFVFDNQILVQAIARGLALGEISCPTRYENDSSSISLRRSI
                     RYGVGVLHTTLAFALWRARLASPAFLDFSADRYLGPTFPADGGISTASPRRSSRRSRR
                     RS"
     gene            complement(4837..6453)
                     /locus_tag="VFV55_07035"
     CDS             complement(4837..6453)
                     /locus_tag="VFV55_07035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX4944093.1"
                     /translation="MHRLFLPLALAVAFGLAAWLRLDQLASQVLIEDEWHPVHQVIYL
                     SPLRILTSFGNADYGIPLVLLYFLQARLVGLSELGLRLPMVAAGLATVAFLPLMLRRR
                     LDDRVLATFALLLALSPFLVSYSRIARSYAITLVGIYAAFWCLERATREGAFAWRHAW
                     RYSVLSGLVVWTHAVTGPMLVAPLAALWWGAMRGRGPAWRTLVALTALTAACMGLAVV
                     PPLFGDLQALAGKSGIDQIKPETIVGAWYLWVGTGSSVIALTSLALAVVGFAAVWRAS
                     PVARWIVLGALFTVAVLVMTKPWWVDRSLALGRYLLPALPVVLLAISAGLIASADAAT
                     GFIATQRRVHPAWALAVVAIAVLAWWPQSPHGELLQRPNSYTQHSFFQYDYRVEKNPI
                     RIGQLTYPASGFWATLASSPPGSLTIAVAPFQYATWEWPAPLWERASGQRVIPAYLWG
                     TCEARRYGEVPPDGRFHFDNAVYVANGWPLAARPVHYLAFYRGPARKGMSPPLPQCEA
                     WMRLRFGQPFHEDEALVVWKNPSPVPAAPD"
BASE COUNT         1163 a         2277 c         2276 g          953 t
ORIGIN      
        1 cagcctctgg gccgaaaagc ccggcgggcc cgtgcacatc atcgaccaga cgcggctgcc
       61 gcacgcgttc gagacgcgcg cgctggattc gcccgaggcg gtggcggaag cgatcgagac
      121 gatgcgcgtg cgcggcgcgc ccctcatcgg cgtcacggcg gccttcgggc tggcgcttgc
      181 catgtcgcgc gacagctccg acgaatcgct ggcgaagacc gccgcgcggc tcgccgccac
      241 ccgccccacg gccgtaaacc tcgcctgggc gatcgagcgg atgcgcgaag ccctcgacgg
      301 cctcgcgccc catgagcggc ccgcggccgc gtggttcgaa gcgcaggcca tcgccgacca
      361 ggacgcggcg ctcaacgaag ccatcggccg acacggcctg gtcctcatcg agcgcgagca
      421 cgcggcgcgc agccgcacgg tgaacatcct cacccattgc aacgcgggct ggatcgccac
      481 cgtggactgg ggcacggcgc tcgcccccat ctacatggcg cacgacaagg gcatccccgt
      541 gcacgtgtgg gtggacgaaa cgcgcccgag gagccagggc ctgctcacgg catgggaact
      601 ggcgggccac ggcgtgccgc acacgctggt ggcggacaac gccggcggtc acctcatgca
      661 gcacggcaag gtggatctcg tgctggttgg cgcggaccgc gtgacgcgcc gcggcgacgt
      721 ggccaacaag atcggcacct acctgaaggc actcgcggcg cgcgacaacg acgtcccgtt
      781 ctatgtcgcc gtccccactc ccaccatcga ctgggagacc gaggacgccc tcgccggcat
      841 ccccatcgag gagcgaagcg gcgaggagat gcgcgtcgtg cgcggcctcg acggatccgg
      901 gcgccttgcc tcggtcgcga tcgcgcagga tacgacgccc gtggccaacc ccgccttcga
      961 cgtgacgccc gccgcgctgg tgacgggcat catcaccgag aagggcgtgt tcgccgcgaa
     1021 ggaagccgcg ctcctcgcgc tttatcccag cgagcgcagg agcaaggcat tcctcacgtg
     1081 aacgacgccg accttcgcga gcgaatcgtc gccacggccc ttcgcatgaa cgccctgggc
     1141 atcaatcagg gaagctcggg caacgtgagc gcacgcgtcg agggcgggtt cctggtgacg
     1201 ccctcgggac tgccctacga ggacttgcag gacgccgaca tcgtgcacgt gggaatgggc
     1261 gggcacgcga cggggcaccg cgagccctcc agcgaatggc ggttccatcg cgacatctac
     1321 gcgacgcggc ccgaggccgg cgcagtcgtg cacacgcatt cggcgttcgc aaccacgctc
     1381 gcgtgcctgg gccgtcccat cccggccttc cactacgagg tcgcgttcgc gggcggcaac
     1441 gatatccgct gcgcgcccta tcgcaccttc ggcacgcagg aactttccga tgcggcgctg
     1501 gctgcgctgg aggaccggcg cgcttgcctc ctcgcgcatc acggcatcat cgccttcggc
     1561 tccgatctcg acgacgcgtt gcggcttgcg gacaaggtcg aggcgctggc gcgcctctac
     1621 tggcaggcca tccaggtcga tgagcccacc gtcctcgacg acgccgagat ggcgcgcatc
     1681 gtcgagcgct tccgccacta cggtccccga agcgggtagg aaggaaatgt accgggtaca
     1741 tgtaccgggt acatgtaccc ggtacgtata attgccggcg aacatattgg aggacgtcct
     1801 atgcacgccc aaagcgcccc ggttccggga gccgcccaca cccgcaacgc aaggcttctc
     1861 aagtgggtcg cggacgtcgc cgccctcacc cagcccgacg cgatctactg gtgcgacggc
     1921 acggaagagg aatacgaccg cctctgcgcc cagctcgtgg aggccggcac cttcaggcgg
     1981 ctgaacgaca agctgcggcc caattcgtac ctggcatggt ccgacccctc cgatgtcgcg
     2041 cgggtcgagg accgcacgtt catctgctcg aagcagaagg acgacgccgg ccccaccaac
     2101 aactgggtcg agcccgccga gatgcgcgcc acgctcaagg gcctgttcag cggctgcatg
     2161 cgcggccgca cgatgtacgt ggtgcccttc agcatgggtc ccatcggctc gcacatcgcg
     2221 cacatcgggg tggagatttc ggacagcgcc tacgtcgccg tgaacatgaa gctgatgacg
     2281 cgcatgggcg caagcgtcct ggcgaccctc ggcgaccacg gcaacttcgt cccctgcgtg
     2341 cattcggtgg gcgtcccgct ggagccgggc gacaacgacg tgccgtggcc gtgcaacagg
     2401 gagcacaagt acatcgtgca gttccccgag gaccgggaga tctggtccta cggctccggc
     2461 tacggcggca acgccctgct gggcaagaag tgcttcgcgc tgcgcatcgc ctcggtgatg
     2521 ggccgcgagc agggctggct cgccgagcac atgctcatcc tgggcgtcga gtcgcccgag
     2581 ggcgtgaagc actacgtgac cgccgccttc cccagcgcct gcggcaagac caacttcgcg
     2641 atgctcatcc cgccggccgt cttcaagggc tggaaggtga cgaccatcgg cgacgatatc
     2701 gcatggatca agccggggga ggacgggcgc ctgcacgcca tcaacccgga ggcgggtttc
     2761 ttcggcgtgg cgccgggcac gggctacgac accaacccga actgcatgga agccgtcaag
     2821 accaacacca tcttcaccaa cgtggcgctt acgcccgagg gcgacgtgtg gtgggagggg
     2881 atgacgaagg aggtgcccgc gaagctcacc gactggacgg gcaaggactg gacgcccggc
     2941 tgcggtcgcc cggccgctca cccgaactcg cgcttcaccg tggccgccac gcaatcgccc
     3001 tcgatcgacc cggcgtggga cgatcccaat ggcgtgccga tctccgcatt cgtcttcggc
     3061 gggcgacgct ccaccaccgt gccgctggtg acggaggccg ccacctggga cgagggcgtc
     3121 tacatggccg ccacgctcgg ctcggaaacg accgccgcca tcaccggcaa ggtgggggtg
     3181 gtgcgccgcg acccgttcgc gatgctcgcc ttctgcggct acaacatcag cgactacttc
     3241 gcccactgga tcgagaccgg aaggaagatc gccctgccgc ccaagatcta tctcgtcaac
     3301 tggttccgca aggacgacga gggcaaatac gtgtggcccg gctacggcga gaacatgcgc
     3361 gtgctcaagt ggatcgtcga gcgcgtcgag gggctggccg acgccgagcg caccgccgtg
     3421 ggctacgtgc cccggcacaa ggacatcgac tggcggggga tggactcctt cggcgccgac
     3481 aagtacctcg ccatcaccgc catcgacaag gcgatgtgga agcaggaaat gccgctgcac
     3541 cgcgagttct tcgacaagct cggcgagcgc ctgccggcgg tgatgcgcca gcgcttccag
     3601 gcgcttgcag gcaccctcgg gtaatcccgc gcgcgcctcc ggcgggcaat ccatcggcgc
     3661 cttgccagcc gtgaacgcga cgctcgccga ggcggggcgg ttcctgcgct atggcatcgc
     3721 gggactcgtg gcgaccgcga cccactacgc ggtcatggtg gcgctcgtct ggaccttcgc
     3781 aggccacgag gtgctcgcct cgtcgatggg cttcatcgcc ggcgccttcg tgaaataccc
     3841 gctcaactat tggctggtgt tcgcgagccg ccagcgccac cgggtcgcgg tgccgcgctt
     3901 cgtgatcggg ctggcgctcg cgttcgccct caacgccgtc ctgcttgcgc tcctgttgcg
     3961 ctcgctcgac gcgcactaca tggtgtcgca ggtgctcacg accggcgtcg tgatcttcgc
     4021 gaactacgtc gtggcgaggc tgtggatatt ccgccatcgg ccgggaacgt cgggcccagg
     4081 tagcgatcgg cgctgaagtc caggaacgcc gggctggcaa ggcgcgcccg ccacagggcg
     4141 aacgcgaggg tcgtatgcag cacgccgacg ccgtagcgga tggaacggcg caggctgatg
     4201 gaactcgagt cgttctcgta acgcgtcggg caggagatct cgccgagcgc aaggccgcgc
     4261 gcgatggcct gcacgagaat ctggttgtcg aacacgaagt cgtcggaatt ggcgagcagg
     4321 ggcagcgact gcagggcctg ccgcgagaag gcgcgatagc cggagtggta ttcggacagc
     4381 ttcgccccgg tgaccaggtt ctggaacgcg gtaagcgccc ggttcgcgcc gtatttccac
     4441 agcggcatcc cgccggacag ggccgctcct ccgaggatgc gcgagccgag gacgacgtcg
     4501 tacacgcccg actcgatcat cgcggccatg gcggtcacga ggcgcggctc gtactggtag
     4561 tcggggtgca ccatgacgac gatgtcggcg ccgtgcgcga gagccgccga ataacaggtc
     4621 ttctggttcc cgccgtagcc gcggttctcg gaatgcatgt ggacgtcgat gcccagcgcg
     4681 cgggcgaccg cggccgtctc gtcgccgctc gcatcgtcga cgaggagcac gtggtccacg
     4741 atcgcgtgcg gcagggcgcg ccaggtcttc tcgacggtcg ctgcggcgcg ataggcgggc
     4801 atcaccacga cgaccttctt gccgttgatc atggtctcag tccggcgcgg caggaacggg
     4861 cgacggattc ttccacacca ccagcgcttc gtcctcgtgg aagggctgcc cgaaacgcag
     4921 gcgcatccag gcctcgcact ggggcagcgg cgggctcatg cctttccttg ccgggcccct
     4981 gtagaaggcg aggtaatgga cgggccgcgc cgcgagcggc cacccgttcg cgacgtacac
     5041 tgcgttgtcg aaatgaaagc gcccatcggg cggaacttcg ccgtagcgcc tggcttcgca
     5101 tgttccccaa aggtatgcgg gtatgacccg ctggccgctt gcccgttccc agagcggggc
     5161 cggccactcc caggtcgcgt actggaacgg ggccacggcg atggtgagcg atccgggcgg
     5221 agaggatgcg agcgtcgccc agaacccgga ggcgggataa gtcagctggc cgatgcggat
     5281 cgggttcttc tccacccggt agtcgtactg gaagaacgag tgctgcgtgt acgagttggg
     5341 ccgttgcagc aattcgccat gcggggactg cggccaccag gcaaggactg caatggccac
     5401 cacggccagc gcccaggcgg gatggacacg gcgttgcgtc gcgatgaacc cggtagcggc
     5461 atcggcgctc gctatcaggc ccgcggagat cgcgagaagg acgacgggca acgcgggcag
     5521 caggtagcgt ccgagggcga gcgaccgatc cacccaccac ggcttggtca tgacgagaac
     5581 ggcaacggtg aacagcgcgc ccaggacgat ccacctcgcg acgggcgatg ctctccagac
     5641 ggccgcaaag ccgacaacgg ccaacgcaag gctggtcaac gcgatcacgc tggagccggt
     5701 tccgacccac aggtaccacg cgccgacgat cgtctccggc ttgatctggt cgatgccgga
     5761 ctttccggcg agtgcctgca ggtcgccgaa aagcggcggc acgacggcca ggcccatgca
     5821 tgccgcggtg agcgccgtca acgccacgag cgttcgccac gccggcccgc ggccgcgcat
     5881 cgctccccac cacagggcgg cgagcggcgc gaccagcatg gggccggtca ccgcgtgggt
     5941 ccagacgacg aggccgctga ggacgctgta gcgccaggca tggcgccatg cgaaggcccc
     6001 ttcccgggtc gcgcgttcca ggcaccagaa cgcggcatag atcccgacca gcgtgatcgc
     6061 gtaggagcgg gcgatgcgcg agtaggaaac gagaaacgga gacagcgcga gcagcagcgc
     6121 gaaggtcgcg agcacccggt cgtcgaggcg gcggcgcaac atgaggggaa gaaacgcgac
     6181 cgtggcgagg ccggctgcga ccatgggcaa gcgcagcccg agctcgctca acccgacgag
     6241 gcgcgcctgc aggaagtaca ggagcacgag gggaatgccg tagtcggcgt tcccgaagct
     6301 cgtcaggatg cgcagcggcg acaggtagat cacctggtgc accggatgcc attcgtcctc
     6361 gatgagcacc tggctcgcca attggtcgag gcgcaaccac gcagccaggc cgaaggcgac
     6421 tgccaacgcc agcgggagga acaagcggtg catcgtggat tgaatcgtcg aagagcgcgt
     6481 aatcataccc cgtgagcgcc tgcgtccccg caatgtcgcg tggaggcgac gccggcgttt
     6541 tcgcaccggc ggaagcaggt aacatgcgcc ggaagtctcc ttcacgaacg atgccatgct
     6601 cccccgcgga caagaatgaa tctcgccgac ctcgaaacgc cgcgcctggt cctcgacgcg
     6661 gaccgcatg
//
LOCUS       DASUEV010000217         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_989626, whole genome shotgun sequence.
ACCESSION   DASUEV010000217 DASUEV010000000
VERSION     DASUEV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989626"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VFV55_07040"
     CDS             complement(<1..1603)
                     /locus_tag="VFV55_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX4944094.1"
                     /translation="MALKQLDEEQVRTWTRAQKDEWWFSNVFRGDMPQLTIRSAATGF
                     LLGGVLAATGLYISGKTGITIGVGLTSVILAFAIFRILHGSGLASDYTILENNCTQSI
                     ATAAGYVVAPLTSSLAAYMLVTERIVPWWQMIVWMVIVSMIGVLLAFPMKRRFINEEQ
                     LPFPEGRACGVVLDALYTGAAAAGMFKAKLLGYTALFTGLYQMVISDGWMKLVQLKLL
                     QMDKWAGLKEAWVFHERLDAYYYAAAAKGSLWIPKILGTEFRTLGLRLTLDAAMLGVG
                     GLMGVAVATSCLLGAFVNFVILAPLMIQAGDIVQRVSPAGTVVLISRAEIVNQWSLWW
                     GVTMMVVGSLVSLAGRPELFRSAFKSLRGRKEAGSDVLAHIEFPLWISAAGVPVFSVL
                     GAWVTHEFFGVPWLLAFVSLPLIFVLTVICTNSMALTSWTPTGSLAKITQFTMGAIDR
                     TNPASNLIPAGMTAEIASNAANLLSDIKPGYMLGAKPRQQAVGHVIGLFAGALACVPL
                     YFILFLPADANGLRSTATIVSEQFAM"
     gene            complement(1625..2047)
                     /locus_tag="VFV55_07045"
     CDS             complement(1625..2047)
                     /locus_tag="VFV55_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4944095.1"
                     /translation="MYSMRVRSVMERGKRVVALPGTTVAKAAKMMAKKNVGAVMVVEE
                     KHLVGIFTERDVVIRVVAEGRDVATTRIGDVMTPSPITIGPDEVFGRALLLMHDNGFR
                     HLPVVEQGEPIGIVSSRSALDPEMEEFVSETQRRLNLR"
     gene            2243..>3156
                     /locus_tag="VFV55_07050"
     CDS             2243..>3156
                     /locus_tag="VFV55_07050"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX4944096.1"
                     /translation="MTRLTLFGAGKIGEAIVHLLSRTGDYRLRAVDADLRRLEPMRRD
                     GVDVVAADIRDEAELGRLVKGQDAVMSACPYYLTPSIARAARREGAHYLDLTEDVAST
                     REVMALGEGADRALIPQCGLAPGFISIVAHDLASRFDVLRDVTMRVGALPTYPSNALK
                     YNLTWSTDGLINEYCNPCIAIRDGEAIETIPLEEVERFSLDGVDYEAFNTSGGLGTLG
                     DTLAGRVENLNYKTVRYPGHRDIVKMLVRDLRLDRRRDLLKDVLEAAIPMTLQDVVLI
                     FVSVTGERQGRLEQTTYAKKIYAQEIDGR"
BASE COUNT          547 a         1069 c         1056 g          484 t
ORIGIN      
        1 gcatggcgaa ctgctccgaa acgatcgtcg ccgtcgagcg caggccgttc gcatccgccg
       61 gcaggaagag tatgaagtag agcggcacac aggcgagcgc gccggcgaac aggccgatta
      121 cgtgcccaac cgcctgctgg cgcggcttgg cccccagcat gtagccgggc ttgatgtcgg
      181 agaggaggtt tgcggcattg gaggcgattt ccgccgtcat gcccgcgggg atgaggttgc
      241 tcgccgggtt ggtgcggtcg atcgcgccca tggtgaactg ggtgatcttg gcgagcgacc
      301 cggtgggcgt ccacgaggtg agcgccatcg agttggtgca gatcacggtg agcacgaaga
      361 tgagcggcag cgacacgaag gcgagcagcc acggcacgcc gaagaactcg tgcgtcaccc
      421 acgcgcccag cacgctgaac acgggcacgc ccgccgccga aatccacagc gggaactcga
      481 tgtgcgcgag cacatccgag cctgcctcct tcctgccgcg cagggacttg aaagcgctcc
      541 ggaacagctc cggccggccc gcgaggctca ccagcgagcc caccaccatc atggtgaccc
      601 cccaccacag cgaccactgg ttgacgatct cggcgcgcga gatgagtacc acggtgccgg
      661 cgggcgagac gcgctgcacg atgtcgcccg cctggatcat gagcggggcg aggatgacga
      721 agttcacgaa ggcgcccagc aggcagctcg tggccaccgc gacgcccatg aggccgccca
      781 cgcccagcat cgccgcatcc agcgtgaggc gcagcccgag ggtcctgaat tccgtgccga
      841 ggatcttcgg gatccacagg cttcccttgg ccgccgccgc gtagtagtag gcgtccagcc
      901 gttcgtggaa gacccaggcc tccttgagtc ccgcccactt gtccatttgc aggagcttca
      961 gctgcacgag cttcatccag ccgtcgctga tgaccatctg gtacaggccg gtgaagagcg
     1021 cggtgtaccc caggagcttc gccttgaaca tgcccgccgc ggcggcaccg gtgtagagcg
     1081 cgtccagcac caccccgcag gcgcggccct ccgggaaggg caactgttcc tcgttgatga
     1141 agcggcgctt catcgggaag gcaaggagca cgccgatcat ggacacgatc accatccaca
     1201 cgatcatctg ccaccacggc acgatccgtt ccgtcaccag catgtaggcg gcgaggctgg
     1261 acgtgagcgg cgccaccacg tagcccgccg ccgtggcgat cgactgggtg cagttgttct
     1321 cgaggatcgt gtaatcggac gcgagccccg agccgtgcag gatgcggaag atggcgaagg
     1381 cgaggatcac cgaggtgagg cccacgccga tggtgatgcc ggtctttccc gagatataga
     1441 ggcccgtggc cgccagcacg ccgcccagca ggaatccggt ggcggccgag cggatcgtga
     1501 gctgcggcat gtcgccgcgg aacacgttgc tgaaccacca ctcgtccttc tgcgcgcggg
     1561 tccaggtgcg gacttgctcc tcgtcgagct gcttgagcgc catggatccc gggacgagga
     1621 cgccctagcg caggttcaac cggcgctgcg tctcggaaac gaactcctcc atctccgggt
     1681 cgagcgcgct cctcgacgag acgatgccga tcggctcgcc ctgctcgacc acgggcaggt
     1741 ggcggaagcc gttgtcgtgc atgaggagca gcgcccggcc gaagacctcg tccgggccga
     1801 tggtgatggg cgagggcgtc atcacgtcgc cgatccgcgt ggtggcgaca tcgcgcccct
     1861 cggcgacgac gcggatgacg acgtcgcgct cggtgaagat ccccaccagg tgcttctcct
     1921 cgaccaccat gacggcaccc acgttcttct tcgccatcat cttcgcggcc ttggcaacgg
     1981 tcgtcccggg cagcgcaacg acgcgcttgc cgcgttccat gacgctcctt acccgcatgc
     2041 tgtacatggg aacctccttc gcgacgaggc tagagcctgt gggtcgcgtc ggcttgacga
     2101 ggatcaacta cccgggatct tgcgccggaa aagccgcgga atgcgctcct ttcgccttcg
     2161 ccgacgccgc cgatatgggc gatatggcgc gggatggccg tccccggtac gctggagagg
     2221 tcttccaccc gcgggagcgg ccatgacccg cctcaccctc ttcggcgccg gcaagatcgg
     2281 cgaggccatc gtccacctgc tgtcgcgcac gggcgactac cggctgcgcg ccgtcgatgc
     2341 cgacctgcgg cggctcgagc cgatgcggcg cgacggtgtc gacgtggtcg cggcggacat
     2401 ccgcgacgaa gccgagctgg gccgcctcgt gaaaggccag gatgccgtca tgagcgcctg
     2461 cccctattac cttacgccat cgatcgcccg ggccgcccgg cgcgagggcg cgcactacct
     2521 cgaccttacc gaggacgtgg ccagcacgcg cgaggtgatg gcgctgggag agggagccga
     2581 tcgcgcgctg attccgcagt gcggcctggc gccgggcttc atctcgatcg tggcccacga
     2641 ccttgccagc cgcttcgacg tgctgcgcga cgtgacgatg cgcgtggggg cgctgccgac
     2701 ctaccccagc aacgccctca agtacaacct cacgtggagc accgacgggc tcatcaacga
     2761 gtactgcaac ccctgcatcg ccattcgcga cggcgaggcg atcgagacga tcccgctgga
     2821 ggaggtcgag cgtttctcgc tcgacggggt ggactacgag gcgttcaaca cctcgggggg
     2881 tcttggcacc ctgggcgaca cgctggccgg acgcgtggag aacctcaact acaagacggt
     2941 gcgctacccc ggccatcgcg acatcgtgaa gatgctcgtg cgcgacctgc gcctcgaccg
     3001 ccggcgcgac ctgctgaagg acgttctcga ggccgcgatt ccgatgaccc tgcaggacgt
     3061 cgtgctgatc ttcgtctcgg tgacgggcga gcgccaggga cgcctcgagc agacgaccta
     3121 tgcgaagaag atatacgcgc aggagatcga cggccg
//
LOCUS       DASUEV010000218         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_635926, whole genome shotgun sequence.
ACCESSION   DASUEV010000218 DASUEV010000000
VERSION     DASUEV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635926"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2931
                     /locus_tag="VFV55_07055"
     CDS             <1..2931
                     /locus_tag="VFV55_07055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478641.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX4944097.1"
                     /translation="LIEGKAIQLHPLVCAAFNADFDGDQMAVHVPISLEAQMEARTLM
                     LSSNNVLHTAHGEPVIVPSQDIVLGLYYATRERIDAREDERNPIDEQRLKAAGDRPLQ
                     FFSNVTEVSRAYESGAVKLHAKIMVRIREFRREESGEFTEVIARHETTVGRALLSEIL
                     PAGLPFTIINKPLKKKEISKLINEAFRRCGIRETVIFADKLMYAGFTYATRAGLSICV
                     DDLLIPVEKSKIIDESEREVKEIERQYTSGLVTQGERYNKVVDIWGRAGDQIAKAMMD
                     QLGREKVVDRNGKETTQESFNAIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIET
                     PITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVVIEEDCG
                     TVQGMAMKALVEGGEVVEPLRERILGRVTATDLLHPESQQVVIAAGTLLDESGVERIE
                     SLGIDEVKVRTPLTCDTRYGLCSKCYGRDLGRGALVNVGEAVGVIAAQSIGEPGTQLT
                     MRTFHIGGAASRAAVASSFEAKSNGMVRFAPTMRYVTNARDEQIAISRNGEIVIADDN
                     GRERERHKVPYGATLSVRDGAKAKAGQTLATWDPLTRPIITEYAGEVRFENVEEGVTV
                     AKQIDETTGLSSLVVIDPKRKAGAAAKGLRPAVKLLGDKGKEVKLAGSDTPVNYTFQI
                     GAIVTVKNGQQLNVGDVLARIPQESSKTRDITGGLPRVAELFEARSPKDAGLLAEVTG
                     TVSFGKDTKGKQRLIITDFDGAAHEYLIPKDKHISVHDGQVVNKGEGIVDGPSDPHDI
                     LRLQGMEALARYIIDEVQDVYRLQGVKINDKHIEVIVRQMLRRVQVVDPGETRFITGE
                     QVERAEYLAENDRVKADGKRPASFEYMLLGITKASLSTDSFISAASFQETTRVLTEAA
                     IMGKKDDLRGLKENVIVGRLIPAGTGLAYHSTRKKDAEADASASTTFDFSVDDADVGS
                     EQVA"
     gene            3047..3424
                     /gene="rpsL"
                     /locus_tag="VFV55_07060"
     CDS             3047..3424
                     /gene="rpsL"
                     /locus_tag="VFV55_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX4944098.1"
                     /translation="MPTINQLLRKPRQKAVTKSKVPALGGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGYEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHMVRGSL
                     DTAGVKDRKQSRSKYGAKRPKAA"
BASE COUNT          676 a         1188 c         1194 g          475 t
ORIGIN      
        1 ctgatcgagg gcaaggcgat ccagctgcac cccctcgtct gcgccgcgtt caacgccgac
       61 ttcgacggcg accagatggc ggtgcacgtg cccatctcgc ttgaggcgca gatggaggcg
      121 cgcacgctca tgctctcctc caacaacgtt ctgcacaccg cgcacggcga gccggtgatc
      181 gtgccctcgc aggacatcgt gctcggcctc tactacgcca cccgcgagcg gatcgacgcg
      241 agggaagacg agcgcaaccc catcgacgag cagcggctga aggcggccgg cgacaggccg
      301 cttcagttct tctccaacgt gaccgaggtg agccgcgcct atgaatccgg cgccgtgaag
      361 ctgcatgcga agatcatggt gcgcatccgc gaattccggc gcgaggaatc gggcgagttc
      421 accgaggtga tcgcgcgtca cgagacgacg gtcggccgcg ccctcctgtc ggagatcctg
      481 cccgcggggc tgccgttcac gatcatcaac aagccgctca agaagaagga gatctcgaag
      541 ctcatcaacg aggccttccg ccgctgcggc atccgcgaga cggtgatctt cgccgacaag
      601 ctgatgtatg cgggcttcac ctatgcgacc cgcgcgggcc tgtcgatctg cgtcgacgac
      661 ctgctcatcc ccgtcgagaa gtcgaagatc atcgacgaga gcgagaggga agttaaggag
      721 atcgagcgcc agtacacgtc cggcctcgtg acccagggcg agcgctacaa caaggtggtc
      781 gacatctggg gccgcgccgg cgaccagatc gcgaaggcga tgatggacca gctcggcagg
      841 gagaaggtcg tcgaccgcaa cggcaaggaa accacgcagg agtccttcaa cgccatctac
      901 atgatggccg actcgggcgc gcgcggctcg gcggcgcaga tccgccagct cgcgggcatg
      961 cgcggcctga tggccaagcc cgacggctcc atcatcgaga cccccatcac ggcaaacttc
     1021 cgcgaggggc tgaacgtgct gcagtacttc atctcgacgc acggcgcgcg caagggcctg
     1081 gcggacacgg cgctcaagac cgccaactcc ggctacctca cccgccgcct cgtcgacgtg
     1141 acgcaggacc tggtcgtgat cgaggaggat tgcggcaccg tccagggcat ggccatgaag
     1201 gcgctggtcg agggaggcga ggtggtcgag cccctgcgcg agcgcatcct cggccgcgtc
     1261 accgccaccg acctgctcca ccccgagtcg cagcaggtgg tcatcgcggc cggcacgctg
     1321 ctcgacgaga gcggcgtcga gcgcatcgag tcgctcggca tcgacgaggt gaaggtgcgc
     1381 acgccgctca cctgcgacac ccgctacggc ctttgctcca agtgctacgg ccgcgacctc
     1441 ggccgcggcg cgctggtgaa cgtgggcgag gccgtgggcg tcatcgccgc gcagtcgatc
     1501 ggcgagcccg gcacgcagct cacgatgcgc accttccaca tcggcggcgc ggcgtcgcgg
     1561 gccgcggtcg cgagctcgtt cgaggccaag tccaacggca tggtgcgctt cgcgcccacc
     1621 atgcgctacg tgacgaacgc ccgcgacgag cagatcgcga tctcgcgcaa cggcgagatc
     1681 gtcatcgccg acgacaacgg ccgcgagcgc gagcgccaca aggtccccta cggggccacg
     1741 ctgtcggtgc gcgatggcgc gaaggccaag gccggccaga cgctcgccac gtgggacccg
     1801 ctcacccgcc cgatcatcac cgagtacgcg ggcgaggtgc ggttcgagaa cgtcgaggag
     1861 ggcgtgaccg tggcaaagca gatcgacgag accacgggcc tgtcgagcct ggtcgtgatc
     1921 gatccgaagc gcaaggcggg cgccgcggcg aagggcctgc gccccgcagt gaagctgttg
     1981 ggcgacaagg gcaaggaggt gaagctcgcc ggcagcgaca cgccggtgaa ctacacgttc
     2041 cagatcggcg cgatcgtcac ggtgaagaac ggccagcagc tgaacgtggg cgacgtcctg
     2101 gcgcgcatcc cgcaggagtc ctcgaagacg cgcgacatca cgggcggcct gccgcgcgtg
     2161 gccgagctct tcgaggcgcg ctccccgaag gacgcggggc tgctcgcgga agtcaccggc
     2221 accgtctcgt tcggcaagga cacgaagggc aagcagcgcc tcatcatcac cgacttcgac
     2281 ggggctgccc acgagtacct gatcccgaag gacaagcaca tctcggtgca cgacgggcag
     2341 gtggtcaaca agggcgaggg catcgtcgac gggccctccg acccgcacga catcctgcgg
     2401 ctgcagggga tggaggcgct tgcccgctac atcatcgacg aggtgcagga cgtctatcgc
     2461 ctccagggcg tgaagatcaa cgacaagcac atcgaggtga tcgtgcgcca gatgctgcgc
     2521 cgcgtgcagg tcgtcgaccc gggcgagacg cgcttcatca ccggcgagca ggtcgagcgc
     2581 gcggagtacc tggccgagaa cgaccgcgtc aaggcggacg gcaagcggcc ggcgagcttc
     2641 gagtacatgc tgctcggcat caccaaggcg tcgctttcca cggactcgtt catctccgcg
     2701 gcctccttcc aggagaccac gcgggtgctc accgaggcgg cgatcatggg caagaaggac
     2761 gacctgcgcg gcctcaagga gaacgtcatc gtcggccggc tcatcccggc gggaacgggc
     2821 cttgcgtacc actcgacgcg caagaaggac gccgaggcgg acgcgagcgc gagcacgacc
     2881 ttcgacttct ccgtggacga cgcggacgtc ggcagcgagc aggttgcttg actggaatga
     2941 gctaaacccc taaacttgag agcttttttc cgccgccttc cttctgtgaa ggcggcggag
     3001 ccgggcttcc gagagggggg ccggaacgac catcggacga aaaaacatgc ccacgatcaa
     3061 tcagttgctc cgcaagccgc ggcagaaggc cgtcaccaag agcaaggtgc cggcgcttgg
     3121 cggcagcccc cagaagcggg gcgtgtgcac gcgcgtgtac acgacgaccc cgaagaagcc
     3181 gaactcggcg ctgcgcaagg tggccaaggt gcgcctcacc aacggctacg aagtcatcag
     3241 ctacatcggc ggggaggggc acaacctcca ggagcactcg gtggtgctca tccgcggcgg
     3301 ccgcgtgaag gacctgccgg gtgtgcgcta ccacatggtt cgtggcagcc tcgacaccgc
     3361 gggcgtcaag gaccgcaagc agtcgcgctc gaagtacggc gccaagcggc ccaaggccgc
     3421 ctgatatcgc gcggccgccg gccgtccgtc gcgcggactt ccgcgcgcca cgaaacaacc
     3481 agatcatcaa ggaacgatcg ccatgcctcg tcgtcgcgaa gtaccgaagc gcg
//
LOCUS       DASUEV010000219         7800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_990339, whole genome shotgun sequence.
ACCESSION   DASUEV010000219 DASUEV010000000
VERSION     DASUEV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990339"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFV55_07065"
     CDS             <1..681
                     /locus_tag="VFV55_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4944099.1"
                     /translation="YRKVESDDWMRAQMRIASLIAKRDGLAEGRDYLQKLEPRTQDDR
                     IQIVQMEAQLLRDAKAWQDTFDMLSSAVERIPDSFELLYDRAMAAERVNRLDILEADL
                     RRVIQLKPDYAHAYNALGYTLADRTDRIDEAYVLIEKALKLTPDDPFILDSLGWVQYR
                     RGKLDEALGYLKSAYQARPDPEIAAHLGEVLWKLGRKDEASNLWKAALTDYPGHETLL
                     AVIQKFKP"
     gene            707..1225
                     /locus_tag="VFV55_07070"
     CDS             707..1225
                     /locus_tag="VFV55_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEX4944100.1"
                     /translation="MTRFATMAAAAFVAACATAPRVPIPLDGLPAAFEMGGRLSVAQS
                     GQGEILRIRWSHALSDDTWVLATPVGTEVARIERDHAAEGLTVLRPGAEPMSAASFAE
                     LTQSLLGAALDERLLVAWLHGRPLAGPEGWAVTIDESQRIGEADVARRITATRGDTVV
                     KLVVDQYRAQPE"
     gene            1230..2078
                     /gene="ispE"
                     /locus_tag="VFV55_07075"
     CDS             1230..2078
                     /gene="ispE"
                     /locus_tag="VFV55_07075"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX4944101.1"
                     /translation="MRLDGPDYPAPAKLNLFLHVVGRRADGYHLLQSAFALIDAADRL
                     RFSVRGDGAIRRVSDLTGVPAETDLVVRAARLLKAETGTALGADIEVDKLLPLGGGVG
                     GGSSDAATTLIVLNRLWDTGLDRPALQALGARLGADVPFFVFGRNAWVEGVGERLVPL
                     DLPPAWYLVLVPPVAVPTGEIFAAPELTRNTEALKMEDFSARTGQGRFRNDLEAVVAA
                     RYPAVREHLEWLASHGGGRLTGSGACVFARFEDRASAERVLEKLPRPMQGFVAQGLGQ
                     HPLRGM"
     gene            2119..2195
                     /locus_tag="VFV55_07080"
     tRNA            2119..2195
                     /locus_tag="VFV55_07080"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2153..2155,aa:Gln,seq:ttg)
     gene            2251..3201
                     /locus_tag="VFV55_07085"
     CDS             2251..3201
                     /locus_tag="VFV55_07085"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX4944102.1"
                     /translation="MPFDNLMVFTGNANPKLAQKVARHLNVQLGKASVSKFSDGEIMV
                     ELLENVRGKDVFVLQSTCAPTNDTLMELLIMVDALRRSSVARITAAMPYFGYARQDRR
                     PRSARVAISAKVVANMLTTAGVNRMLTMDLHADQIQGFFDIPVDNVYATPILLGDLWK
                     HGYKDLVVVSPDVGGVVRARAIAKRLESDLAIIDKRRPRPNVATVMNIIGDVDGRTCV
                     IMDDMVDTANTLCEAARALKERGAVRVVAYCTHPVLSGPAISRIESSVIDELVVTDTI
                     PLRDDAQACTKIRQISVSELLAETMRRICEESSVSSLFVE"
     gene            3315..4034
                     /locus_tag="VFV55_07090"
     CDS             3315..4034
                     /locus_tag="VFV55_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX4944103.1"
                     /translation="MKIEIKAEPRSLQGTGASRRLRRESKVPGILYGAGVDSQPIQLD
                     HKELFFKLKLEAFHASILDMDLGSEKTQVLLRDYQMHPFKQMILHADFQRVAADRKIH
                     MKVPLHFVNADISPGVKLAGGQVQHVQNDLDIACLPKDLPEFIEVDLADLQPGHSLHL
                     SGLKLPAGVESTALSKGEDLTVATVVIPRVAAADEEVVEAAAAAPSAADVPTTAQKVK
                     EEEPAKEAGKGGGKDKDEKKK"
     gene            4140..4730
                     /gene="pth"
                     /locus_tag="VFV55_07095"
     CDS             4140..4730
                     /gene="pth"
                     /locus_tag="VFV55_07095"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX4944104.1"
                     /translation="MEPIRLIVGLGNPGREYERTRHNAGYWWADAIAHDHGGRWAKES
                     KFSGWVARVAEGGRDFWLLKPATYMNESGRSVGAFLHFHRIEPRQMLVVHDELDLPPG
                     TVKLKFGGGTGGHNGLRDIDEVLGTRDFWRLRIGIGHPGHKDMVADYVLHEARRAEQE
                     AIDPAFDRSLGLLPRIATGRMTDATTWLHTLPKDEQ"
     gene            4727..5818
                     /gene="ychF"
                     /locus_tag="VFV55_07100"
     CDS             4727..5818
                     /gene="ychF"
                     /locus_tag="VFV55_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX4944105.1"
                     /translation="MTLQCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVV
                     EVPDPRLAAIAAIAHPLKILPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETNAIAN
                     VVRCFEDDNVVHVAGKVDPISDIEVIHTELALADMATVDKARDRSIQKSRSGDKEAAK
                     FVAVLEKVQKALDGLHPVRALGLAPDEMELLKPLFLLTAKPALYIANVEEHGFVDNPL
                     LEKVKEYAAKEGAPVVAICAKLESEIADLSGEEKELFLADMGMHEPGLDRLVRATFGL
                     LGLQTYFTAGEKEVRAWTIHKGDTAPQAAGVIHTDFEKGFIRAEVIAYDDYIACKGEL
                     RAKEAGKMRLEGKEYVVKDGDVMHFRFNV"
     gene            5869..5944
                     /locus_tag="VFV55_07105"
     tRNA            5869..5944
                     /locus_tag="VFV55_07105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5902..5904,aa:Met,seq:cat)
     gene            complement(5992..>7800)
                     /locus_tag="VFV55_07110"
     CDS             complement(5992..>7800)
                     /locus_tag="VFV55_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX4944106.1"
                     /translation="RAAQMRALEEKPADKLISGMQLLAMVAAQAKTGSIASDAYRSEV
                     GRIIAAEVRRDDQSVLDGYDIADKSAQRGLLGVLAQIDFLEGRYAQAGKRATEMRALE
                     EKPADKLISGMQLLAMVAAQAKTGSIASDAYRSEVGRIIAAELAKLPYPVIANDIKGM
                     KSSAEIIGEALVLGNVRDRLQPVVDKAGGVLGSDFAPAIVGARYALVARLPLKQTLIE
                     TYSAYLAANKVEKPDIWAARAVELPKGRNYAPVKIAVWDSGVDRALFRDRLALNAAGK
                     PAFIAFDRYENPSSSDMMPIPAELQGKLPEMKSRIKGLSDLQSNVESDEASEVKALLS
                     KLKRDEYRGVVEELGLVSNYSHGTHVAGIAMAGNPYARLVNARIEFGYKLLPEPCPSR
                     ELVEKSARNSLSYVNFLKRNGVRVVNMSWGGTVREIEGELELCNIGKTPDERKQIARE
                     YFDMQKKALSAAFASAPEILFVTSAGNSNQDSTFAEAIPAELVLPNLITVGAVDKAGD
                     EAPFTSYGPTVKVHANGYQVESYLPGGDRVALSGTSMSSPQVTNLAAKILAVKPKLTP
                     AEVIAIIRKTAERTADGRRFLIDPAKAVATAQARSG"
BASE COUNT         1312 a         2578 c         2641 g         1269 t
ORIGIN      
        1 taccgcaagg tcgagtcgga cgactggatg cgcgcgcaga tgcggatcgc ctctctcatc
       61 gcgaagcgcg atggccttgc ggagggccgt gattacctgc agaagctcga gccgcgcacc
      121 caggacgatc gcatccagat cgtccagatg gaagcgcagc ttctgcgcga cgccaaggcc
      181 tggcaggaca cgttcgacat gctgtcgagc gcggtggagc gcatcccgga ttccttcgag
      241 ctcctctatg accgtgccat ggcggcggag cgggtgaacc gcctggacat cctcgaggcg
      301 gaccttcgcc gcgtgatcca gctcaagccc gactacgcgc acgcctacaa cgccctcggc
      361 tacacgcttg ccgaccgcac cgaccggatc gacgaggcct acgtcctcat cgagaaggcg
      421 ctcaagctca cgccggacga cccgttcatc ctcgacagcc tgggctgggt ccagtaccgc
      481 cgcggcaagc tcgacgaggc gctcgggtat ctcaagagcg cgtaccaggc acgcccggac
      541 ccggaaattg ccgctcacct gggcgaagtg ctgtggaagc tcggccgcaa ggacgaggcg
      601 agcaacctgt ggaaagcggc gctcaccgac tacccgggcc acgagacgct gctcgcggtg
      661 atccagaagt tcaagccttg agggcccgcg gcccgcgccc ttgtccgtga cccgcttcgc
      721 cacgatggcc gcggccgctt tcgtcgcggc gtgcgccacc gcgccccggg tgcccattcc
      781 gcttgacgga cttcccgccg cgttcgagat gggggggcgc ctgtccgtcg cgcagtcggg
      841 gcagggcgag atcctgcgca tccgctggag ccatgcgctt tccgacgaca cgtgggtgct
      901 cgccacgccg gtcgggacgg aggtggcgag gatcgagcgc gaccacgccg cggaagggct
      961 gaccgtcctt cgccccgggg cggaacccat gtcggcggca agttttgccg aattgacgca
     1021 atccctcctc ggcgcggccc tcgacgagcg ccttctcgtt gcctggctgc atgggcggcc
     1081 gctggccggt ccggagggct gggccgtgac gatcgacgaa tcgcagcgca tcggggaagc
     1141 ggacgtggcg cggcgcatca cggcgactcg cggggacacg gtggtgaagc tggtggtcga
     1201 ccaataccgg gcgcagcccg aatagcccca tgcgactgga cggaccggac taccccgcgc
     1261 ctgcgaagct caacctcttc ctgcacgtgg tcgggcgccg ggcggacggc taccacctgt
     1321 tgcagagcgc ttttgccctc atcgatgcag ccgaccgcct gcgattttcc gtgcgcggcg
     1381 acggggcgat ccggcgggtg agcgacctga caggcgtgcc ggccgagacc gaccttgtcg
     1441 tgcgggcggc ccggctgctc aaggccgaaa ccgggacggc actcggcgcc gacatcgagg
     1501 tcgacaagct tttgccattg ggcgggggcg taggcggcgg cagctcggac gccgctacga
     1561 cgctcatcgt gctcaaccgc ctgtgggata ccgggttgga caggccggcc ctgcaggccc
     1621 tgggcgcccg cctgggggcc gatgtcccgt ttttcgtctt tggccgaaac gcctgggtcg
     1681 aaggcgtggg cgaaaggctt gtccccctgg accttccccc ggcctggtat ctcgtgctgg
     1741 tgccgccggt ggcggtgccc acgggggaga tctttgccgc gcccgaattg acccggaata
     1801 cggaagccct taaaatggaa gacttttcgg cacgcacagg gcagggacgg ttccggaatg
     1861 acctggaagc ggtcgtggcc gcccggtatc ccgcggttcg cgagcacctc gagtggctcg
     1921 cctcccacgg cggagggcgg ctcacggggt ccggcgcgtg cgttttcgcc cggttcgagg
     1981 accgggcctc ggcggagagg gtgctggaaa agctcccccg gccgatgcag ggattcgtcg
     2041 ctcagggcct cgggcagcac cccctgcgcg gcatgtaacg ggtaataccc gcggccggaa
     2101 gttccgggcg cgggcgccag gggagtcgcc aagctggtta aggcactgga ttttgattcc
     2161 agcattcgaa ggttcgaatc cttcctcccc tgccacatcc ttttgatgcg ccgcccgtaa
     2221 cggggcgcga agcgacgcga gagacgggcg atgcctttcg acaacctgat ggtgttcacg
     2281 ggcaacgcca acccgaagct cgcgcagaag gtggcgcgcc acctgaacgt gcagctgggc
     2341 aaggcgagcg tgtcgaagtt cagcgacggc gagatcatgg tcgagctgct cgagaacgtg
     2401 cgcggcaagg acgtgttcgt gctccagtcg acctgcgcgc cgaccaacga cacgctgatg
     2461 gagctgctga tcatggtgga cgcgctgcgc cgctcgagcg tggcgcggat caccgcggcc
     2521 atgccgtact tcggctacgc ccgccaggac aggaggccga ggagcgcgag ggtggcgatc
     2581 tcggccaagg tggtggccaa catgctcacc accgcgggcg tgaaccgcat gctgacgatg
     2641 gacctgcacg cggaccagat ccagggcttc ttcgacatcc ccgtggacaa cgtctatgcg
     2701 acgccgatcc tgctcgggga cctgtggaag cacgggtaca aggacctggt ggtggtgtcg
     2761 ccggacgtgg gcggcgtggt gcgggcgagg gcgatcgcaa agaggctcga aagcgacctc
     2821 gcgatcatcg acaaacgtcg gccgcggccc aacgtcgcga cggtgatgaa catcatcggc
     2881 gacgtggacg gccgcacctg cgtgatcatg gacgacatgg tcgacacggc caacacgctg
     2941 tgcgaggcgg cgcgcgcgct gaaggaaagg ggtgccgtcc gggtggtggc gtactgcacg
     3001 caccccgtgc tctcggggcc ggccatcagc cggatcgaga gctcggtgat cgacgagctg
     3061 gtggtgacgg acaccatccc gctgcgcgac gatgcgcagg cctgcacgaa aatccgccag
     3121 ataagcgtct cggagctgct cgccgagacc atgcgcagga tctgcgaaga gagctccgtg
     3181 agctcgctct tcgtggagtg acggtttccg ccttgctccc gggaaagcgg gagcgggcaa
     3241 cggggtgagg ggaccgcttc cccctcgaac agcggggcgc ctggtcgcgg ggccctgccg
     3301 acatcggagt gaccatgaag atcgaaatca aagcagaacc gcgaagcctg cagggaacgg
     3361 gtgcgagccg ccgcctgcgc cgcgaatcca aggtgcccgg catcctctat ggcgccggcg
     3421 ttgactcgca gcccatccag ctcgaccaca aggaactctt cttcaagctg aagctggaag
     3481 cgttccacgc ctccatcctc gacatggatc tcggttcgga gaagacgcag gtgctgctgc
     3541 gcgactacca gatgcacccg ttcaagcaga tgatcctgca cgcggatttc cagcgtgtcg
     3601 ccgccgacag gaagatccac atgaaggtgc cgctgcactt cgtgaatgcc gacatctccc
     3661 cgggcgtcaa gctcgcgggg ggccaggtgc agcacgtgca gaacgacctc gacatcgcct
     3721 gcctgcccaa ggacctcccc gagttcatcg aagtggatct cgcggatctt cagccggggc
     3781 actcgctgca cctttccggc ctgaagctcc cggcgggcgt ggagtcgacc gcgctctcca
     3841 agggcgagga cctgacagtc gccactgtgg tgatcccgcg cgtggcggcg gcggacgagg
     3901 aagtcgtgga agctgctgcg gccgcgccgt cggcggccga cgtgccgacc acggcccaga
     3961 aggtgaagga ggaggagccc gcgaaggaag cggggaaagg cggcggcaag gacaaggacg
     4021 agaagaagaa ataacggccg ccgggggcgc atcgatcgac cctcgcggat gcgcccgcct
     4081 ttcccggcgg gcgcgttcgt tttgggggcc accgccctgc aagccctcac gaacgtgaca
     4141 tggaacccat ccgcctcatc gttggcctcg gcaaccccgg gcgcgagtac gaacgcacgc
     4201 gccacaacgc cggctactgg tgggcggacg ccatcgcgca cgaccacggc gggcgctggg
     4261 cgaaggaatc gaaattctcc ggctgggtcg cgcgcgtggc ggagggcggc cgtgatttct
     4321 ggctgctcaa gcccgccacc tacatgaacg aaagcggccg cagcgtgggc gcattcctgc
     4381 acttccaccg catcgagccg cgccagatgc tcgtggtgca cgacgaactg gaccttccgc
     4441 caggcacggt gaagctcaaa ttcggcggcg gcaccggcgg gcacaacggg ctgcgcgaca
     4501 tcgacgaggt gctggggacg cgcgacttct ggcggctgcg catcggcatc gggcacccgg
     4561 gccacaagga catggtggcc gattacgtcc tgcacgaggc gcggcgcgcg gagcaggaag
     4621 ccatcgaccc cgccttcgac cgcagcctgg ggctcctgcc gcgcatcgcc accggccgca
     4681 tgaccgatgc gacaacctgg ctgcacacgc taccgaaaga cgagcaatga ccctgcaatg
     4741 cggcatcgtg ggactcccca acgtgggcaa gtccactctc ttcaacgcgc tcaccaaggc
     4801 cggcatcgcg gccgagaact atccgttctg caccatcgag cccaacgtgg gcgtggtcga
     4861 ggtgccggac ccgaggctcg cggccatcgc ggcaatcgcg catccgctga agatcctccc
     4921 ggcgatcgtc gagttcgtcg acatcgcggg gctcgtggcc ggcgcttcca agggcgaggg
     4981 cctgggcaac cagttcctgg ccaacatccg cgagaccaat gccatcgcca atgtcgtgcg
     5041 ctgcttcgag gacgacaacg tcgtgcacgt ggccggcaag gtggacccca tctccgacat
     5101 cgaggtgatc cacaccgagc tcgcactcgc cgacatggcg accgtggaca aggcgcgcga
     5161 tcgcagcatc cagaaatccc gctcggggga caaggaagcc gccaagttcg tcgccgtgct
     5221 cgagaaggtg cagaaggccc tcgacggcct gcacccggtg cgggcgctgg gcctggcacc
     5281 cgatgagatg gagctcctca agcctctctt cctcctcacc gccaagcccg cgctctacat
     5341 cgcgaacgtg gaggagcatg ggttcgtgga caacccgctg ctcgagaagg tgaaggaata
     5401 cgccgcgaag gaaggcgcgc ccgtcgtcgc gatctgcgcg aagctcgaga gcgagatcgc
     5461 cgatctctcc ggcgaggaaa aggagctctt cctcgccgac atggggatgc acgagcccgg
     5521 cctcgaccgg ctcgtgcgtg cgaccttcgg cctgctgggc ctgcagacct acttcacggc
     5581 cggcgagaag gaggtgcgcg cgtggaccat ccacaagggt gacaccgccc cgcaggcggc
     5641 cggcgtcatc cacaccgatt tcgagaaggg cttcatccgc gccgaggtca tcgcctacga
     5701 tgactacatc gcctgcaagg gtgagctgcg tgccaaggaa gcgggaaaga tgcggctgga
     5761 gggcaaggag tacgtcgtga aggacgggga cgtgatgcac ttccgcttca acgtgtgact
     5821 gcgttccttg acccgtgttc gcaaatgccc gaaaatgcac ggctttccgg cgagttagct
     5881 cagtggttag agcagcggaa tcataatccg ttggtccggg gttcaaatcc ctgactcgcc
     5941 accaccgttt cccgaatcgc cggccggttt ccgaccggcg atttttcttg atcagccgct
     6001 gcgagcctgt gccgtcgcaa cggccttcgc cggatcaatg aggaaccggc gaccgtcggc
     6061 ggtcctctcc gccgtcttcc ggatgatcgc aatcacctcg gccggcgtga gcttcggttt
     6121 gaccgccagg atcttcgccg cgaggttcgt cacctgcgga gaagacatcg acgtgccgga
     6181 cagcgcgacc cggtcgccgc ccggcaggta gctttccacc tggtaaccat tggcgtgcac
     6241 cttgacggtg ggtccgtagc tggtgaacgg cgcttcgtcg cccgccttgt cgaccgcgcc
     6301 cacggtgatc aggttgggca gcacgagttc cgccggaatg gcttccgcga acgtggagtc
     6361 ctggttggaa ttgccggctg aagtgacgaa caggatctcc ggcgcgctcg cgaatgcggc
     6421 gctcagcgcc ttcttctgca tgtcgaagta ctcccgcgcg atttgcttgc gctcgtcggg
     6481 cgtcttgccg atgttgcaca gttccagttc gccttcgatt tcgcgcacgg ttccgcccca
     6541 gctcatgttc acgacgcgaa cgccgtttcg cttcaggaaa ttcacgtagg acagggaatt
     6601 gcgcgcgctc ttttccacga gctcgcggct cgggcacggt tcggggagca gcttgtagcc
     6661 gaactcgatc cgcgcgttga cgaggcgggc gtaggggttg cccgccatgg cgatgccggc
     6721 cacgtgggtt ccgtggctgt agttgctgac gaggcccagt tcctcgacga cgccgcgata
     6781 ctcgtcgcgc ttgagcttcg acagcagggc cttcacctcg ctcgcttcgt cgctttcgac
     6841 gttggactgc aggtccgaca gccccttgat gcgggacttc atctcgggca gcttgccctg
     6901 cagttcggcg gggatcggca tcatgtcgct ggacgaggga ttctcgtagc ggtcgaaggc
     6961 gatgaacgcg ggcttgcccg ccgcgttgag cgccaggcgg tcgcggaaga gtgccctatc
     7021 gacgccgctg tcccagacgg cgatcttcac cggcgcgtag ttgcgtccct tgggcagctc
     7081 gaccgcgcgt gccgcccaga tgtcgggctt ctcgaccttg ttcgccgcca ggtaggccga
     7141 gtacgtctcg atcagcgtct gcttgagggg cagccgcgcc acgagcgcgt agcgggcgcc
     7201 cacgatggcg ggcgcgaagt cggagccgag cacgccgccc gccttgtcga cgacgggctg
     7261 caggcggtcg cggacgttgc ccagcacgag cgcctccccg atgatctcgg ccgaggattt
     7321 cattcccttg atgtcgttcg cgatcaccgg gtacggcagc ttcgcgagct cggccgcgat
     7381 gatccttccc acctcgctgc gataggcgtc ggaggcgatg cttcccgtct tcgcctgcgc
     7441 ggcgaccatc gcgagcagct gcatgcccga gatgagcttg tcggcgggct tctcctcgag
     7501 cgcacgcatt tcggtggcgc gcttgccggc ctgcgcgtag cgcccctcga ggaaatcgat
     7561 ctgcgccagc acgcccagca ggccgcgctg cgcggacttg tcggcgatgt cgtacccgtc
     7621 gagaacggac tggtcgtcac gccgcacctc ggccgcgatg atccgtccca cctcgcttcg
     7681 ataggcgtct gaagcgatgc ttcccgtctt cgcctgcgcg gctaccatcg cgagcagctg
     7741 catgcccgag atgagcttgt cggcgggctt ctcctcgagc gcacgcatct gggcggcgcg
//
LOCUS       DASUEV010000220         7412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1104728, whole genome shotgun sequence.
ACCESSION   DASUEV010000220 DASUEV010000000
VERSION     DASUEV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104728"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1070)
                     /locus_tag="VFV55_07115"
     CDS             complement(318..1070)
                     /locus_tag="VFV55_07115"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX4944107.1"
                     /translation="MKELGRLQSAFADAVLGGDDREFVRQLAGDAGMGQRRLAVYRRA
                     ILANRTGALRAAFPVVARLVGDAFFDEAALRHAEASPPECADLNRYGASFPAFIAGYR
                     HAAAMSWLSDVARLEWAWQESLLAADAPGLDFAALARVPERGHAGMRFSLHPCVRLVR
                     SAWPVLAIWEANQPDRDGTPSREEGADDVLVWREDQRVRLALLTLPEFAFVEGLARGL
                     TLEAAAGLDDGWDFGPMLQRLAEHGMLCGFST"
     gene            complement(1076..1942)
                     /locus_tag="VFV55_07120"
     CDS             complement(1076..1942)
                     /locus_tag="VFV55_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX4944108.1"
                     /translation="MTARFPIRAPDPARAGIGLRTPHFEALAATRPPLSFLEVHSENY
                     FADGGPSIAWLERFRADYAISLHGVGLSLGSADPLDREHLSRLARLAGRIEPALVSEH
                     LCWSSIGGRHLGELLPLPYTEEALAHVCGRIAEAQDLLGRVLLVENVSSYVQFASSTI
                     PEGEFVAEVARRTGCGVLLDVNNAWVNAVNHGTDARDFLAAIEPATVGEIHLAGCEVT
                     PDGLVDTHGAPVFPEVWALFEETIARLGPVPTLVEWDTRIPPLDVLLAEAARADAILD
                     SSVARAAGRVAA"
     gene            complement(1955..2233)
                     /locus_tag="VFV55_07125"
     CDS             complement(1955..2233)
                     /locus_tag="VFV55_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEX4944109.1"
                     /translation="MNSKRALIRSALAGVIAIGLGQAQAVNAADGEPAQGREKCYGIA
                     KAGQNDCGTATHSCAGKSKKDNAPDEWKYVAKGTCEKVGGKLKPPEGK"
     gene            2435..2860
                     /locus_tag="VFV55_07130"
     CDS             2435..2860
                     /locus_tag="VFV55_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX4944110.1"
                     /translation="MNQLLRYEPLTGRLDGLFDDLFRPALRREGGSGPAPIRIDVRET
                     PDAYAVRADIAGVRKEDIHVEIEGNEVLISAEVQRESGKKEGERVLRAERFSGKTARR
                     FALPQEIDQAKAEAKYADGVLELALPKKVAVAGRRVAIQ"
     gene            2978..3886
                     /gene="argB"
                     /locus_tag="VFV55_07135"
     CDS             2978..3886
                     /gene="argB"
                     /locus_tag="VFV55_07135"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX4944111.1"
                     /translation="MPPRLSSAQKAEVLVEALPYIQRFYDKTIVIKYGGNAMTDVALQ
                     EDFAEDIVLLKLIGMNPVVVHGAGPQIGALLQKLGKQSEFIQGMRVTDEETLDVVEMV
                     LGGLINQEIVTLINKAGGKAVGLTGKDGHFIHARKLKVASKEDKKKKIDVGLVGEVVR
                     IDPEIIQLLDERDFIPVIAPIGADDKGTAYNINADVAAGKLAVTLQAEKLILLTNTTG
                     VLDKSGKVITGLTAKKVNEMIDDGTISGGMLPKVECALDAVRNGVKSAHIIDGRVSHA
                     LLLEVLTSEGVGTLIRGSSSNRVDHR"
     gene            complement(3905..5062)
                     /gene="clsB"
                     /locus_tag="VFV55_07140"
     CDS             complement(3905..5062)
                     /gene="clsB"
                     /locus_tag="VFV55_07140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEX4944112.1"
                     /translation="MRPVAGNRVTLLRNGAEFFPALLAAVDSASDDVRLEAYIFADDV
                     VGRAVGEALKRAAQRGAKVRLMVDGFGSRELSPGFIGDLRSAGVVVQLFRPERGWFSF
                     RRSRLRRLHRKIALIDGRIGFVGGINILDDHTGHGSPAPRYDYAVRVEGPLLAEIYPV
                     VHRLWWLVAALTRKERQPGFLPPEVHPAPAGDTAAVFVFRDNFRHRHDIETMYLDAIR
                     GARREILIACAYFMPGWRVRHALMEATARGVRVAIVVQGWSDHRVFQHASRVLYGALL
                     DHGIEIYEYERSEMHAKAAVADGRWATVGSSNLDPFSLFLAREANVAVLSEAFARELR
                     ESIDHEIHHGARAVPRLLWKRRPWITRFAGWLAYGYARLAMGIAGIGRRWY"
     gene            complement(5082..5840)
                     /locus_tag="VFV55_07145"
     CDS             complement(5082..5840)
                     /locus_tag="VFV55_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX4944113.1"
                     /translation="MKTGADPALTVVTYNIHKGFSALNRRLVVHEIRERLHALAPDVV
                     FLQEVQGLHRKHSRRHITWPEASQYEYLSQEGDHSVYGMNATYLDGHHGNAILSRFPI
                     VMSENIDISHHALESRGLLHCEMAVPGWKVPLHCINVHLGLWARSRRFQLEWLCDRIR
                     ASVPKRAPLVVAGDFNDLWGTATAVLEQELGLAEVFERSLGRHAKSFPARLPLLALDR
                     IYVRDLKVGVTQRLAEGPWSRLSDHAALAARLSR"
     gene            complement(5837..6304)
                     /gene="nudB"
                     /locus_tag="VFV55_07150"
     CDS             complement(5837..6304)
                     /gene="nudB"
                     /locus_tag="VFV55_07150"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX4944114.1"
                     /translation="METRFKIPVSTLVVIYTAGLDVLLIERADRPGFWQSVTGSQDPG
                     ETLAQTAIREIGEETGIDATRYALADWQVTNVFEIFPVWRHRYAPGVTHNTEHVFGLR
                     VPDRVDVRLAPDEHLRFRWLPWQEASREVFSWSNREAILMLPDKDSLSARTAT"
     gene            complement(6309..7232)
                     /locus_tag="VFV55_07155"
     CDS             complement(6309..7232)
                     /locus_tag="VFV55_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HEX4944115.1"
                     /translation="MTIALIAHGGAGKWRPGSDDDAVEGLRAAVESGRAILARGGSAL
                     DAVCATVVALEDNPIYNAGTGAVLNFDGFCEMDACVMESGQARVGAVSGLQRVRNPIL
                     VARKVMDETDHVMLTGDGAQRFARVMGFPDHDPVTPERRADWFDKRNRIDEILGVQAL
                     KMRRFLKEHPEYAGGTVGAAAVDASGVLAAATSTGGVTLKMVGRVGDSPVPGAGNYAS
                     RRVAASATGTGEFVLRSLATRAIAERVEGGSSLSQAMAAVLDRLGADFDADVGFIAVD
                     DGGLPVAMHRTRDMPHAFFSGDAAVVSRMRA"
     gene            complement(7229..>7412)
                     /locus_tag="VFV55_07160"
     CDS             complement(7229..>7412)
                     /locus_tag="VFV55_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944116.1"
                     /translation="LVALMAGVEAIRDVMAFPKTQRGQDLLTGAPSAVTEKQLRELHI
                     RLRSAQAAPAAGEVKA"
BASE COUNT         1267 a         2560 c         2490 g         1095 t
ORIGIN      
        1 accggcgcca aagatctgcc ttctcctggc ccgtcaacat cggccgattg cttcgcctca
       61 tatgcaccac ccccttgaaa gtgattcaga ataacggggt gttgcatcga cccattgaat
      121 ccgccggccc aaaacaaaaa acaagacaca tccaacctca ccccgaacaa gcctctcgct
      181 tcgacgccgg tgataacgga gttctgccgc gaaggttccc tcaagcatcg atcaccgtga
      241 agtcgtgtcg atcgaactcg cgattggtgg tgacgatcga ggttgccatg ggcgttggtg
      301 gcggtttgcg cggacgctca tgtggagaag ccgcacagca tgccgtgctc ggcgagacgc
      361 tggagcatcg ggccaaagtc ccacccgtcg tccagcccgg ccgccgcctc gagcgtcaag
      421 ccgcgcgcga gtccttcgac gaaggcgaat tcgggcagcg tgagcagcgc caggcgcacg
      481 cgttggtcct cgcgccagac gagaacgtcg tccgcgccct cttcgcgcga cggcgttccg
      541 tcgcggtccg gctggttcgc ctcccagatc gcgagcacgg gccaggccga gcgaacgagg
      601 cgaacgcagg gatgaaggga aaatcgcatg cccgcatggc cgcgttccgg cacgcgcgct
      661 agggcggcga aatcgagacc cggcgcatcc gccgccagca gcgattcctg ccacgcccac
      721 tcgaggcggg cgacgtcgga gagccacgac atcgccgccg cgtgccggta tccggcgatg
      781 aacgccggga atgacgcgcc gtagcgattg aggtccgcgc attcgggagg cgatgcctcc
      841 gcgtggcgca gcgccgcttc gtcgaagaac gcgtcgccca cgaggcgcgc cacgaccggg
      901 aaggcggcgc gcagcgcgcc cgtccggttg gcgagaattg cccttcgata gacggcgagc
      961 cgtcgctgcc ccatcccggc atcgcccgcg agttgccgaa cgaattcccg gtcgtcgcca
     1021 cccaggaccg cgtcggcaaa cgccgactgc aggcggccca gttccttcat tcgcgtcacg
     1081 ccgccacccg gccggccgcg cgcgcgacgg atgaatcgag gatggcgtcg gcccgtgccg
     1141 cttccgccag caggacatcg agcgggggaa tgcgggtatc ccactcgacg agcgtgggca
     1201 ccggccccag gcgcgcgatc gtctcctcga acaacgccca cacctccgga aagacggggg
     1261 cgccgtgcgt atcgacaagc ccgtccggcg tcacctcgca acccgcgaga tggatctcgc
     1321 cgaccgtcgc cggctcgatg gcggcaagga aatcccgggc atcggttccg tggttcaccg
     1381 cattgaccca cgcgttgttc acgtcgagga gcacgccgca acccgtgcgg cgcgccactt
     1441 cggccacgaa ctcgccctcc ggaatcgtgg aggatgcgaa ttggacatag gacgatacat
     1501 tctcgacgag gagcacgcgc ccgaggagat cctgagcctc ggcgatacga ccgcacacat
     1561 gggcaagtgc ctcctccgtg tacggcagcg gcagaagctc gcccaggtgc cggccgccta
     1621 tgctcgacca gcaaaggtgc tccgagacca gcgccggctc gatgcgcccg gcgaggcgcg
     1681 cgaggcgcga caggtgctcg cgatcgagcg ggtcggccga gcccagggag agccccaccc
     1741 cgtgcaggct gatggcgtaa tcggcccgga accgctccag ccaggcaatc gagggcccac
     1801 cgtcggcgaa gtagttctcg ctgtgaacct cgagaaacga gagcggtgga cgcgtggcgg
     1861 ccagcgcctc gaaatgcggc gtgcgcaggc cgatcccggc ccttgcgggg tccggcgcgc
     1921 ggatcgggaa gcgcgcggtc acgcccggcc ttgactactt gccttcggga ggcttcagct
     1981 tgccgccgac cttctcgcaa gtgcccttgg ccacgtactt ccactcgtcg ggcgcgttgt
     2041 ccttcttcga cttgcccgcg caggagtgcg tggccgtgcc gcaatcattc tggcccgcct
     2101 tggcaatgcc atagcatttc tccctgcctt gcgcgggttc gccgtcggcc gcgttgacag
     2161 cctgggcctg gccgaggccg atcgcgatca cgccggccag cgccgaccgg atgagcgctc
     2221 ttttcgagtt catggaaatc tccttttcgg gtgaggtttg cgggcgttcg ccggcggatc
     2281 ccggcgggcg gggaaaaatg atagcaccgg gccggggccg cccgcccctt gaattcccgg
     2341 gcgccggcca aaaattgggt tcatcgggcg ccgattgcgc cccgtggggc ccggcgcgaa
     2401 gccggaaata cgatcaattc aattggaggc aagaatgaac cagctgctgc gctacgagcc
     2461 cctgacgggg cgactcgacg gacttttcga cgacctcttc cgcccggccc tgcgccggga
     2521 aggcgggtcc ggccccgcgc ccatccgcat cgacgtccgc gagacgccgg atgcctacgc
     2581 cgtccgtgcc gacatcgccg gagtgaggaa ggaggatatt cacgtcgaga tcgagggcaa
     2641 cgaggtgctg atcagcgcgg aagtgcagcg cgagagcggg aagaaggaag gcgagcgggt
     2701 gctgcgcgcc gagcgcttct ccggcaagac cgcccggcgc ttcgcgctgc cgcaggaaat
     2761 cgaccaggcg aaggccgagg cgaaatatgc cgatggcgta ctcgaactcg ccctgcccaa
     2821 gaaggttgcg gtggccgggc gccgggtcgc catacagtag cgatcgcctt tccctgccgg
     2881 ggtccggggg gccgcgcccg ccgggcccct ttttacttgg agggctgccg cctacaatag
     2941 cggccactcc cccctccctc accgagcctt tcccgccatg ccccccaggt tatcctccgc
     3001 ccaaaaggcc gaagtgctgg tcgaagccct gccgtacatc cagcgcttct acgacaagac
     3061 gatcgtgatc aagtacggcg gcaacgcgat gaccgacgtc gcgctgcagg aggacttcgc
     3121 ggaggacatc gtgctgctga agctcatcgg gatgaacccg gtggtcgtgc acggcgccgg
     3181 gccgcagatc ggggcgctgc tgcagaagct cggcaagcag agcgaattca tccagggcat
     3241 gcgcgtcacc gacgaggaga cgctcgacgt ggtggagatg gtgctcggcg gcctcatcaa
     3301 ccaggagatc gtcacgctca tcaacaaggc cggcggcaag gcggtcggcc ttaccggcaa
     3361 ggacgggcac ttcatccacg cccgcaagct caaggtggcg agcaaggagg acaagaagaa
     3421 gaagatcgac gtcggcctcg tgggcgaggt ggtgcgcatc gacccggaga tcatccagct
     3481 cctcgacgaa cgcgacttca tcccggtgat cgcgcccatc ggcgcggacg acaagggcac
     3541 cgcctacaac atcaacgccg acgtcgcggc cggcaagctc gcggtcacct tgcaggccga
     3601 aaaactcatc ctgctcacca acacgaccgg cgtgctcgac aagtcgggca aggtgatcac
     3661 cgggctcacc gccaagaagg tgaacgagat gatcgacgac ggcaccatct ccgggggcat
     3721 gctgcccaag gtcgagtgcg cgctggatgc ggtcaggaac ggcgtgaagt ccgcacacat
     3781 catcgacggg cgcgtctcgc acgccctgct gctggaggtg ctcaccagcg agggtgtcgg
     3841 cacgctcatt cgcggctcgt cgtccaaccg cgtggatcac cgctaggcgg cgacgctcta
     3901 cccgtcagta ccaacgccgg ccgatgccgg cgatgcccat cgcgagccgc gcgtacccgt
     3961 aggcgagcca tcccgcgaag cgcgtgatcc acggtcggcg cttccacagc aggcggggca
     4021 ccgcgcgcgc gccgtggtgg atctcgtggt cgatggattc gcgcagctcc ctcgcgaacg
     4081 cctcgctcag caccgccacg ttggcttccc gggcaaggaa caggctgaac ggatcgagat
     4141 tggacgaacc taccgtggcc caacgcccgt cggccaccgc ggccttggcg tgcatttcgc
     4201 tgcgttcgta ctcgtagatc tcgatgccgt ggtcgagcag cgcaccgtac aggacgcggc
     4261 tcgcgtgctg gaagacccga tggtccgacc agccctgcac cacgatcgcg acgcgcacgc
     4321 cccgcgcagt cgcctccatg agcgcgtggc gcacccgcca gccgggcatg aagtaggcgc
     4381 aggcgatgag gatttcgcgg cgcgccccgc ggatcgcgtc gaggtacatg gtctcgatgt
     4441 cgtggcgatg ccggaaattg tcgcggaaga cgaataccgc cgccgtgtcc ccggcaggag
     4501 cggggtgcac ctcgggaggc aggaatccgg gctggcgctc cttgcgcgtg agcgcggcca
     4561 ccagccacca gagccggtga acgacggggt agatctcggc gagcagcggt ccctcgacgc
     4621 gcaccgcgta gtcgtagcgc ggcgccggcg atccatgccc ggtatggtcg tcgaggatat
     4681 tgattccgcc cacgaagccg atgcgtccgt cgatgagcgc gatcttccgg tgcaggcgcc
     4741 ggagccggct tcgccggaag gagaaccagc cccgttccgg gcggaagagc tgaaccacca
     4801 cgcctgcgct gcgcaggtcg ccgatgaatc ccggcgaaag ctcgcgcgaa ccgaacccgt
     4861 cgaccatcag gcgcaccttg gcgccccgtt gcgccgcgcg cttcagcgcc tcgcccacgg
     4921 cacgaccgac cacgtcgtcg gcgaaaatgt aggcctcgag acgtacgtcg tcggaggcgg
     4981 agtcgacggc ggccagcagc gccgggaaga actcggcgcc gttgcgcagg agcgtcacgc
     5041 gattgccggc aaccggcctc accgggcctc cgggggcacc ctcaacgcga cagcctcgcg
     5101 gcgagcgccg cgtggtcgga aaggcgggac cacggcccct ccgcgaggcg ctgcgtaacg
     5161 cccaccttca ggtcgcgcac gtagatgcgg tcgagcgcga gcaacggcag ccgcgccggg
     5221 aagctcttcg cgtgccggcc aagggatcgc tcgaacactt ccgcgaggcc cagctcctgc
     5281 tcgagcaccg cggtcgcggt tccccacagg tcgttgaagt ccccggcgac cacgagcggt
     5341 gcgcgcttcg gcacggaagc acggatgcga tcgcaaagcc attcgagctg gaagcggcgg
     5401 ctgcgcgccc acaggccaag gtggacgttg atgcagtgca gcggcacctt ccacccggga
     5461 accgccatct cgcaatgcag gagcccccgg ctctccaggg cgtggtgcga gatgtcgatg
     5521 ttctccgaca tcacgatcgg aaaccggctg aggatcgcgt tgccgtggtg cccgtccagg
     5581 taggtagcgt tcatgccgta gaccgaatgg tccccctcct gcgacaggta ctcgtactgg
     5641 ctcgcctcgg gccacgtgat gtggcggcgc gaatgcttgc ggtgcaaacc ctgcacttcc
     5701 tgcaggaaca ccacatccgg ggccaacgca tgcagccgtt cgcggatctc gtgaaccacc
     5761 aggcggcggt tgagcgccga gaagcccttg tggatgttgt acgtgacgac ggtgagggcc
     5821 gggtcggctc cggttttcat gtcgcggttc gggcggacag cgaatccttg tcgggaagca
     5881 tcagtatcgc ctcacgattg ctccatgaaa acacctcgcg cgacgcctcc tgccacggca
     5941 accagcggaa gcgcaggtgc tcgtcggggg cgagccgcac gtcaacacga tccgggaccc
     6001 gcagcccgaa gacgtgctcg gtattgtgcg tcacgcccgg ggcgtagcgg tggcgccaga
     6061 ccgggaagat ttcgaagacg ttggtaacct gccagtcggc gagcgcgtag cgtgtcgcgt
     6121 cgatgcccgt ctcctcgccg atttcgcgga tggcggtctg cgcgagcgtc tctccgggat
     6181 cctggcttcc ggtgacgctc tgccagaagc cggggcggtc ggcgcgttcg atgaggagca
     6241 cgtcgagccc ggccgtgtag atcacgacga gcgtcgagac ggggatcttg aagcgcgtct
     6301 ccaatcagct aggcccgcat gcgcgacacc acggcagcgt cgcccgagaa gaaagcgtgc
     6361 ggcatgtcgc gcgtgcggtg catggcaacc ggcaggcccc cgtcatcgac ggcgatgaag
     6421 cccacgtccg catcgaagtc cgcgcccagc cggtccagca cggccgccat ggcctgcgag
     6481 aggctcgagc cgccctcgac ccgctccgcg atggcacggg tggcgagcga gcgcaggacg
     6541 aattcgccgg tgcccgttgc cgaggcggcc acgcgccgcg aggcgtagtt gcccgcgccc
     6601 gggaccgggg aatcgcccac gcgccccacc atcttgagcg tcacgccgcc ggtggaagtg
     6661 gccgcggcca gcaccccgct cgcgtccacc gcggcggcgc ccacggtgcc cccggcgtat
     6721 tcgggatgct ccttgaggaa gcggcgcatc ttgagcgcct gcacgccgag gatctcgtcg
     6781 atgcgattcc tcttgtcgaa ccagtcggcg cgacgctcgg gcgtgaccgg atcatggtcc
     6841 gggaatccca tgacgcgggc gaagcgctgc gcgccgtcgc cggtgagcat gacgtggtcg
     6901 gtttcgtcca tgaccttccg cgcaacgagg atggggttcc tcacgcgctg cagcccggac
     6961 accgcgccca cccgcgcctg gccgctttcc atgacgcagg catccatctc gcagaagccg
     7021 tcgaagttga ggacggcgcc ggtgcccgcg ttgtagatgg ggttgtcctc gagcgccacg
     7081 accgtcgcgc acaccgcgtc gagtgcgctc cccccgcgcg cgaggatcgc gcgcccggat
     7141 tcgaccgcgg ctcgcagccc ttccaccgca tcgtcgtcgg agccggggcg ccacttgccc
     7201 gcgcctccgt gcgcgatgag agcgatcgtc acgccttcac ctctcctgcg gcgggagcgg
     7261 cctgcgcgct gcgcagccgg atgtgcagct cgcgcaactg cttctcggtt acggccgagg
     7321 gcgcgccggt cagcagatcc tggccgcgct gcgtcttggg aaacgccatg acgtcgcgga
     7381 tcgcctcgac gcccgccatc agcgccacca gg
//
LOCUS       DASUEV010000221         5421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_990616, whole genome shotgun sequence.
ACCESSION   DASUEV010000221 DASUEV010000000
VERSION     DASUEV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990616"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..3172)
                     /locus_tag="VFV55_07165"
     CDS             complement(35..3172)
                     /locus_tag="VFV55_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4944117.1"
                     /translation="MWITRTSINQPVFATMMMVALCVLGIFSYNRLRVERMPDIEIPF
                     VFIQIQYPGAAPEAVENDITKPVEEVVNTVNGVKIIRSNSFEGRSETYIEFRLDTNME
                     RAVQDVRDKIAMIRPTFPREARDPLVLRGDFDNAQPVVSLAVTSPTRSLRSLTTLTDQ
                     VIVKRFQNVPGVGQVQVSGGVARQVLVNLRPAQMQAQSVGVDEIVAAIRAANMDVPAG
                     KIVHGPAEQLVRIEGRIKEPAGFGKIIVARRAKGPVRLDQVADIVDGEREADSLSRIN
                     GQPGLTIAVLKVQDANIVEVGNGAKAAAEALKKVLPPDVELKLVYADVDFIEKALNGV
                     KTTIVEGALLTVFIVFLFLHSWRSTIITGLTLPISVIATFIAVHLFGFTLNFLTLMAL
                     SLCIGLLIDDAIVVRENIVRHLGMGKDHYSAARDGTDEIGLAVMATTFAIVAVFVPIA
                     FMSGIIGKFFFQFGVTVAVAVLVSLFVSFTLDPMLSAIWRDPPEGRFRRVPWLGRMMG
                     RIENGVEWAHHRYGELLEWALANRKKVLAIAVAVFVSSFFVLPLVGTEFVPEADEGRT
                     SFRLTTPVGSSLEYTDAKTRQVEEILRRFPEVEVMAANVGTGEGRNVARIEVRLSPRG
                     QRPHLSQKAFEAKVREKIRAIPGIELAVGYNRPIWINLLGPSNEGLETVSNEVMDRIG
                     RIKGITDLESSLKANNPAITIKVNDELASDLGLTVAQIGAAVRPLVAGDVIGHWLAPD
                     GQNYEVNVQLPKSDRRIAADLGELHVVSSKPGPDGAPILVPLRQVVEFRNSTSPQIIK
                     RQELQRRVGIYANVEGRPSGDVGKEVQEMLKTITLPPGYRFDVAGQTQDMQESFTAAV
                     AALGMAVIFIYIILASQFGSFLQPLAIMASLPFTLIGVFLALLVTGSTLNIFSIIGII
                     MLMGLVTKNAILLVDFTNHGRREGKSQHDAILAAGQVRLRPILMTTMAMIFGMLPMAI
                     GSTDGGEQNGPMGRAVIGGIITSTLLTLVVVPVLYTYLDAWGRRFSAWFARGAPAGHA
                     GGALPMQAPSKPADD"
     gene            complement(3183..4373)
                     /locus_tag="VFV55_07170"
     CDS             complement(3183..4373)
                     /locus_tag="VFV55_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4944118.1"
                     /translation="MIFRKSSSPAADPSGRLRRRLGKPVLILLVLVLVAGSLIAFRGS
                     LAKKEDSTKGAAPTLEFTPADIAVVELRALERTVAFSGSLSPVTQSTVRSKVPGEIRR
                     VLVREGDKVARGQLLAEIDTADLQARLDAQLAALEEARAKLSIAAKNRENGLQLLKQG
                     FISQNASDTTQSAFEAAAAGVKSAEAQVRLARNATQDAIVRSPIDGIVAKKMINAGEK
                     VGIDSALVTLVDLARMEIEAPAPAAEIPAIRIGQSAAFRVDGFGDRAFAGRLERINPV
                     TDPGSRSIMIYLSVVNPDGVLRGGMFAKGSLILDKSVPAPTIPASAVREEAGQTFVFT
                     VEQGRIVRREVTLGLQEDQAGLVEVRKGLEKGVAVVSSRAMSLKPGAPAVLKGSPAPA
                     AKAS"
     gene            complement(4478..>5421)
                     /gene="gluQRS"
                     /locus_tag="VFV55_07175"
     CDS             complement(4478..>5421)
                     /gene="gluQRS"
                     /locus_tag="VFV55_07175"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX4944119.1"
                     /translation="GGLAAVVTRAMGYIGRFAPSPTGPLHFGSLVAAVASWLDARSVG
                     GRWHLRIEDVDAPRTAPGAEDDILRTLEALGLAWDGPVVRQRERKARYEEALETLRGR
                     GLTYRCRCSRREIADSGVHGIDGVVYPGTCRHAAVASTTPAAERFIVPDETVVLDDRV
                     QGRVAQRLATDIGDFVVHRRDGLFAYQLAVVVDDGDLGVTDVVRGADLLDSTPRQVAL
                     QRALGLRTPRYLHVPVATRGGEKLSKQTLAPAVVASSGTAALLSALDFLGQAAPETDR
                     PAAVLAQAATRWDPARIPRGRTREITDGHGTGYPLAV"
BASE COUNT          835 a         1821 c         1898 g          867 t
ORIGIN      
        1 tgcaactcaa agtaacctga ccccttttcg gggttcagtc gtccgcaggc ttcgagggcg
       61 cctgcatggg aagggcgcct cccgcgtgcc ctgccggggc acccctggcg aaccaggccg
      121 agaacctgcg gccccacgca tcgaggtagg tgtagagaac cggcaccacc accagggtga
      181 ggagggtgga ggtgatgatg ccgccgatca ccgcgcggcc catcggcccg ttctgctcgc
      241 cgccatcggt cgaaccgatc gccatcggta gcatcccgaa gatcatcgcc atcgtcgtca
      301 tcaggatcgg gcgcagccgc acctggcccg cggcgaggat cgcgtcgtgc tgcgacttgc
      361 cctcgcgccg gccgtggttg gtgaagtcga cgagcaggat cgcgttcttg gtcaccagcc
      421 ccatcagcat gatgatgccg atgatcgaga agatgttgag cgtgctgccg gtgaccagca
      481 gcgcaagaaa cacgccgatc agggtgaagg gcagcgacgc catgatcgcc agcggctgca
      541 ggaagctgcc gaattgcgag gcgaggatga tgtagatgaa gatcaccgcc atgccgaggg
      601 ccgcgaccgc cgccgtgaac gattcctgca tgtcctgggt ctggcccgcg acgtcgaagc
      661 ggtagcccgg cggcagggtg atggtcttga gcatttcctg cacttccttg cccacgtcgc
      721 ccgagggccg gccctcgacg ttggcataga tgcccacgcg ccgctgcagt tcctggcgct
      781 tgatgatctg cgggctggtc gagttgcgga actccaccac ctggcgcagc ggcacgagga
      841 tcggcgcgcc gtcgggaccc ggcttgctgc tcacgacgtg cagctcgccc aggtcggcgg
      901 cgatgcgccg gtccgatttc ggcagctgca cgttcacctc gtagttctgc ccgtcgggcg
      961 cgagccagtg gccgatgacg tcgcccgcca cgagcgggcg cacggcggcg ccgatctgcg
     1021 cgacggtgag gccgagatcg ctcgcgagtt cgtcgttgac cttgatcgtg atggccgggt
     1081 tgttggcctt gaggctgctc tcgaggtcgg tgatgccctt tatcctgccg atccggtcca
     1141 tcacctcgtt cgacaccgtc tccagcccct cgttggacgg cccgaggagg ttgatccaga
     1201 tcggccggtt gtagcccacg gcaagctcga tgcccgggat cgcgcggatc ttctcgcgca
     1261 ctttcgcctc gaacgccttc tgcgacaggt gcggcctctg gccgcgcggc gacaggcgca
     1321 cctcgatgcg ggcgacgttg cgcccttcgc ccgtgccgac gttggcggcc atcacttcca
     1381 cctcgggaaa ccggcgcagg atctcctcga cctggcgcgt cttggcgtcg gtgtactcga
     1441 ggctcgagcc caccggcgtg gtgaggcgga acgaggtccg gccctcgtcg gcctcgggaa
     1501 cgaactcggt gccgactagc ggcagcacga agaagctgct gacgaacacc gccaccgcga
     1561 tcgccagcac cttcttgcgg ttcgcgagcg cccattcgag cagctcgccg tagcggtggt
     1621 gcgcccactc caccccgttc tcgatgcgac ccatcatgcg cccgagccac gggacgcggc
     1681 ggaagcggcc ctccggcggg tcgcgccaga tggccgagag catggggtcg agggtgaagc
     1741 tcacgaagag cgacaccagc accgccaccg cgaccgtcac gccgaactgg aagaagaact
     1801 tgccgatgat ccccgacatg aacgcgatcg gcacgaacac cgcgacgatc gcgaacgtgg
     1861 tggccatgac ggcgaggccg atctcgtcgg tgccgtcgcg cgcggcgctg tagtgatcct
     1921 tgcccatccc gaggtggcgg acgatgttct cgcgcaccac gatggcgtcg tcgatcagca
     1981 ggccgatgca cagcgacagc gccatcaggg tgaggaagtt gagcgtgaac ccgaagaggt
     2041 gcacggcgat gaaggtcgcg atcaccgaga tgggcagcgt gagcccggtg atgatcgtgc
     2101 tgcgccagga atgcaggaag aggaagacga tgaagacggt gagcagcgcc ccctcgacga
     2161 tcgtcgtctt cacgccgttg agcgcctttt cgatgaagtc cacgtcggcg tagacgagct
     2221 tcagctcgac gtcgggcggg agcaccttct tcagcgcctc cgcggccgcc ttcgcgccgt
     2281 tgcccacctc gacgatgttg gcatcctgca ccttgaggac cgcgatggtg agcccgggct
     2341 ggccgttgat gcgcgagagc gaatcggcct cgcgctcgcc gtcgacgatg tcggcgacct
     2401 ggtcgaggcg cacgggcccc ttggcgcggc gcgccacgat gatcttgccg aacccggccg
     2461 gctccttgat ccggccctcg atgcgcacca gctgctcggc ggggccgtgg acgatcttgc
     2521 ccgcgggcac gtccatgttg gccgcgcgga tcgcggcgac gatctcgtcc acccccacgg
     2581 actgcgcctg catctgtgcc gggcgcaggt tcaccaggac ctggcgcgcc acgccgccgg
     2641 agacctgcac ctgccccacg ccgggcacgt tctggaaccg cttcacgatg acctggtcgg
     2701 tcagcgtggt gaggctgcgc aggctcctcg tgggcgaggt cacggccagc gagacgaccg
     2761 gctgggcgtt gtcgaagtcc ccgcgcagca ccaggggatc gcgggcctcg cgcgggaacg
     2821 tgggacggat catcgcgatc ttgtcgcgca cgtcctgcac cgcccgctcc atgttcgtgt
     2881 cgaggcggaa ctcgatgtag gtctcgctgc ggccctcgaa ggaattggag cggatgatct
     2941 tcacgccgtt gaccgtgttc accacttcct cgaccggctt ggtgatgtcg ttctcgacgg
     3001 cctcgggggc ggcgcccggg tactggatct ggatgaagac gaacgggatc tcgatgtcgg
     3061 gcatgcgctc cacgcgcagc cggttgtacg agaagatgcc gaggacgcac agcgccacca
     3121 tcatcatggt ggcgaacacg ggctggttga tgctggtccg ggtgatccac atggcggacg
     3181 ccctagctcg ccttggcggc gggcgccggg gagcccttca ggacggccgg tgccccgggc
     3241 ttcaggctca tcgcgcgcga gctcaccacc gccacgccct tttccaggcc tttgcgcact
     3301 tccacgagac cggcctggtc ctcctgcagg ccgagcgtca cctcgcgccg gacgatcctg
     3361 ccctgctcga cggtgaacac gaaggtctgg cccgcctcct cgcgcacggc cgaggcggga
     3421 atggtgggcg cgggcacgct cttgtcgagg atgaggctgc ccttggcgaa catgccaccc
     3481 ctcaggacgc cgtccgggtt cacgaccgac aggtagatca tgatcgagcg cgacccggga
     3541 tcggtcaccg ggttgatgcg ttccagtcgc cccgcgaagg cgcggtcgcc gaacccgtcg
     3601 accctgaagg ccgcggactg cccgatgcgg attgccggaa tctccgccgc cggggcgggc
     3661 gcctcgattt ccatccgcgc aaggtcgacg agcgtgacga gggcgctgtc gatgcccacc
     3721 ttttccccgg cgttgatcat cttcttcgcc acgatgccgt cgatggggga tcgcacgatc
     3781 gcgtcctggg tcgcattgcg ggcgagccgc acctgggcct cggcggactt gactcctgcc
     3841 gcggcggcct cgaaggcgct ctgcgtcgtg tcggaggcgt tctgcgagat gaagccctgc
     3901 ttgagaagct gcaggccgtt ctcgcggttc ttcgcggcga tcgacagctt tgcccgggct
     3961 tcctcgagcg ccgcgagctg cgcatcgagc cgggcctgga ggtccgcggt gtcgatttcg
     4021 gcgagcagct ggccccgcgc gaccttgtcg ccttcgcgca cgagcacgcg gcggatttcg
     4081 cccgggacct tcgatcgcac ggtcgactgc gtcacgggcg acaatgagcc ggagaaggcg
     4141 acggtgcgct ccagcgcgcg aagctccacg acggcgatat cggccggcgt gaactccagc
     4201 gtgggtgccg cgcctttcgt ggagtcctcc ttcttggcga ggctgccgcg gaacgcgatg
     4261 agcgaaccgg cgacgaggac gaggacgaga aggatcagca ccggcttgcc cagccggcgc
     4321 cgcaggcgcc ccgaggggtc ggcggcaggg gagctcgatt tccggaagat catggctggc
     4381 gtcctggcag tgcgcgtccc gggatgaggg ccgcgctcat gccccgttgg atggcggggc
     4441 cggccggttt ggattcaacc gatgagatag ctgccaatta tacggcgagg gggtacccgg
     4501 ttccgtgacc atctgttatt tcgcgggtcc ggccgcgggg tatgcgcgcc gggtcccagc
     4561 gcgtcgcggc ctgggcgagc accgcggcgg ggcgatccgt ctccggcgcc gcctggccca
     4621 ggaagtcgag cgccgaaagc agggcggccg ttccggaaga cgcgacgacg gccggtgcga
     4681 gggtctgctt ggaaagtttt tcgccgccgc gggttgcaac gggaacgtgc aggtaacgcg
     4741 gcgtgcgcag tcccagagcg cgctgcagcg ccacctgccg gggagtggaa tcgaggaggt
     4801 cggcgccgcg caccacgtcg gtcacgccca ggtcgccgtc atcgaccacc acggcgagct
     4861 gataggcaaa aaggccgtcg cgacggtgga cgacgaaatc gccgatgtcg gttgcgaggc
     4921 gctgcgcgac gcgcccctgc acgcgatcgt cgagcaccac ggtttcgtcg ggaacgatga
     4981 agcgctccgc cgccggcgtg gttgacgcaa ccgcggcgtg gcggcacgtg ccgggataga
     5041 cgacgccgtc gatgccgtgc acgccggaat cggcgatctc ccttcgcgag cagcggcagc
     5101 gatacgtgag cccgcggccg cgcagcgttt cgagcgcctc ctcgtagcgg gccttgcgct
     5161 cgcgctggcg cacgaccggg ccgtcccagg cgaggccgag cgcctcgagc gtgcgcagga
     5221 tatcgtcctc ggcaccggga gcggtgcgcg gcgcgtcgac gtcctcgatg cgcaggtgcc
     5281 atcgcccgcc cacggagcgc gcgtcgagcc agctcgccac cgcggccacc agcgatccga
     5341 agtgcagcgg tcccgtgggg gagggcgcga agcggccgat gtagcccatg gctcgcgtca
     5401 cgacagcagc gaggccgcca c
//
LOCUS       DASUEV010000222         8656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1442268, whole genome shotgun sequence.
ACCESSION   DASUEV010000222 DASUEV010000000
VERSION     DASUEV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442268"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFV55_07180"
     CDS             <1..1005
                     /locus_tag="VFV55_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4944120.1"
                     /translation="PIANPRHMVPDWTFGIREPELQKAVDEARAKGARVVVLLSHNGM
                     DVDLKLASRVTGIDAILGGHTHDGIPQPVEVRNAKGVTLVTNAGSNGKFLGVMDFEVS
                     DGRIAGWKYRLLPVFANLLAPDPAMAALIAKVRGPHEARLAEKLAVTEGLLYRRGNFN
                     GTVDQVILDSLMQVKGAEIAFSPGFRWGTTLLPGQAITMDHVMDWTAITYPQTTVNEF
                     TGEMIKTILEDVCDNLFNPDPYYQQGGDMVRVGGLTYTCTPGETMGKRISDMRLAGKP
                     IAADRKYKVAGWAPVAEGARGEPIWDVVTAYLKDRKSIPPVKLNLPKLVGMEGNAGIA
                     "
     gene            1263..2186
                     /locus_tag="VFV55_07185"
     CDS             1263..2186
                     /locus_tag="VFV55_07185"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX4944121.1"
                     /translation="MHLAQASSYEIVTREDFSDVTFLLEVRHPMLAKAAKAGQFVIVM
                     EHEQGERIPLTIADFDPKAGTITLVIQAVGKTTREMQQNCRVGTTLHAVVGPMGIPSH
                     IGSKKKVVCVGGGLGVAPIFPQARAYKESGAYVIGVLGFRNRGLVFWEDKFRACCDEL
                     ILCTDDGSAGIKGLVTEGIKQAIAKHPDIEELVAIGPPIMMKGCAEATRPHKIKTMVS
                     LNPVMVDGTGMCGGCRVKIGETIKFACVDGPDFDAHLVDFDDLMARLGRYRPAEKQAM
                     ERWQESCRIRGEGAQAPEPAVAMDDRPSTDG"
     gene            2243..3748
                     /gene="gltA"
                     /locus_tag="VFV55_07190"
     CDS             2243..3748
                     /gene="gltA"
                     /locus_tag="VFV55_07190"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX4944122.1"
                     /translation="MAKKKTIRTIPQERTPAKEQDPLVRARNFEEVSLGYRLEEALVE
                     AERCLQCADEPCVRGCPVGIDIPGFIQKITEKHYHGAYDIITDTNLLPSVCGRVCPQE
                     NQCEGVCTVGDTLEPVAIGRLERFVGDMAIKEGWANIPYIEPNGYRVGIVGAGPAGMA
                     CAADMAKAGCEVVVYEAFHQPGGVLKYGIPDFRLPNAVIDAEIDKLRALGIRFECNTL
                     VGRLFTIEQMITEMGFHAVFLGTGAGYPSFMGIPGESLNGVLSANELLTRANLMRARD
                     FPNFDTPLQPGKCVTVIGAGNTAMDAMRVSMRLGAEKVYCVYRRTKAEAPARAEEVHH
                     AEEEGIDFNWLTSPIELLGDASNAVRAMRCIRMELGEPDDSGRRRPVPVPGSEFEIAT
                     DMVVFAIGTNANPILGQTAKLKLNKRGYIETDETLATSIAGVYAGGDIVTGAATVIQA
                     MGAGRHAARSMKAYLGLRDSDCVYAPDTDDPTGRRFGIPAEEHNFARVRLL"
     gene            3796..5712
                     /locus_tag="VFV55_07195"
     CDS             3796..5712
                     /locus_tag="VFV55_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX4944123.1"
                     /translation="MSATAQLDRKKQARGNAAPAAGAKPVITLSEHIVEVISDSGEGA
                     QRCGQALAGIAARSGNGIWTVEIIPAEIQPPARSVAGASGIQIRIGSKQVTNGGDETD
                     LVVAFNEQVLLGRVRAGELKPGATILLENAWREHRDPSIVKSFNDTVDALVKGGFRLI
                     EIPLERECRALMTDARRGKNMFVLGMLCSIYSLDLQLARDQIALTFGKKDRSVIDTNV
                     KLLEAGFAWGEANLDFKYEIPAQRSTVAQVVINGNTAIALGVLASGMDICAMYPITPA
                     TSASHYLSDCFEKVGGIVHQAEDEIAACAFAIGASYAGRCAVTITSGPGYSLKQEGIG
                     LAVMAEIPLVVVNVQRGGPSTGQPTKMEQGDMLTAVYGSHGDAPKVVMAVSGIEDCFY
                     SMITARKIAETFNMVVVVLSDASLATAQAPFPRPVFSEDWLAPPLDQSPIPEGARPYD
                     WDPVTGIARRFVPGQPGGMHTVTGLAHDRSSKVAYDPSINEESLRARSLKLAALQKTL
                     TTPPVFGDPEGDLLVVGWGSTKGVIEEAVERLRAEGLKVSSLHMRFIQPMPSGIGEIL
                     KRFRKVMTVEAGWGDRLEDELIDENNRRYTALAMMLRSRFLVDVDCVSEARGQPIKPG
                     NVVKALRAKLEEGK"
     gene            5715..6701
                     /locus_tag="VFV55_07200"
     CDS             5715..6701
                     /locus_tag="VFV55_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4944124.1"
                     /translation="MGNALSQCIIRLHEEQHDLEDYQGGVPRWCNGCGDNAILAAVQK
                     LCRDENLAPEKTVFVSGIGCSSRFPHYMKTYGFHGIHGRAFPIAEGVKMARPDLNVFV
                     NTGDGDCCSIGAAHWIHAIRYNMNLTVILHDNHVYGLTKKQASPTSPIGFKSNTTPRG
                     SLLEALNPLTVTLGVQNVSFVAQAVDWMPEVLSDIVSKAFHHRGFSFIRIVQRCPEFL
                     PKMFEPWLHDPGKTLLLTHPNGLQPSADLGRIYKNQREHDPLDINRAREIASLEDPIP
                     VGILYQNPQVPCYEDLRGAGKPRTVDAIRKGLDIELDKFTIWPDDESQQRAA"
     gene            6745..>8656
                     /locus_tag="VFV55_07205"
     CDS             6745..>8656
                     /locus_tag="VFV55_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944125.1"
                     /translation="MAAKFQDQLVFHMTGRRTGEDLAPVDTGSMRPALLAGYRDLTRL
                     RHDYPLVLVESGDVDGFAASLTLLVNRVLETLAPHGIEGERLRKHVLRLEHELRAMLA
                     TGAHGSLADLWTKAAGRLASDADESVAKILEAAGEAIGIDGELVDCDESLPSRFMVHA
                     WRHVQAQKAALFHGHLDNLQRKLSDILRAAFAHSEAGQQPEALRASFGGTQANDFDFA
                     EMSRLVVRGAPKDELPAARRKRIEWALSVLKSQRFHASRTVAAGDDAPYEFLFDNIAA
                     AVAAYRERLAQAVELLKAIAIAELETRNGYDEADHDQFFEAYDKRALTADDLALFPDY
                     LVCIPAERNSAPENAALMDTLSSGLPVKVVVQVTDLLEEASIGTGHYAFGVRSARLAT
                     TAMGLGGMFVLQAASSSLYRMRNRIEGGMGCRGPALFSVFSGAPAAAGEMPRYLTAAA
                     AMESRAFPAFVYDAAGGDNWATRFSLEHNRNPDTDWPVETFEYADGTMQRVREEVAFT
                     YADFALCDRRNAEHFAVVSRERWTAAMVPAGDWLALTERDASDRIPYLLAIDAQDVLH
                     RVVVDIRMMQATRRCLLLWHRLQEHGGIHDSHAERLLAREKAAWEETHKRDLQAAAAN
                     AVPAEVAPAATSA"
BASE COUNT         1563 a         2990 c         2868 g         1235 t
ORIGIN      
        1 ccgatcgcca acccgcggca catggttccg gactggacct tcggcatccg ggaaccggaa
       61 ctgcagaagg ccgtggacga ggcgagggca aagggcgcac gggtggtggt gctcctgtcg
      121 cacaacggca tggacgtgga cctgaagctc gccagccgcg tcacggggat cgacgcgatc
      181 ctgggcgggc acacgcacga cggcatcccg cagcccgtgg aggtgaggaa cgcgaagggc
      241 gtcaccctcg tcaccaacgc cggcagcaac ggcaagttcc tcggcgtgat ggacttcgaa
      301 gtcagcgacg gcaggatcgc cggctggaaa taccggctgc ttcccgtatt cgccaacctc
      361 ctggcgcccg acccggccat ggccgcgctc atcgcgaagg tccgtggccc tcacgaagcc
      421 aggctcgcgg agaagctcgc ggtcacggaa ggccttcttt accggcgcgg caacttcaac
      481 ggcacggtgg accaggtgat cctcgactcg ctgatgcagg tgaagggcgc tgagatcgcc
      541 ttctcgccgg gattccgctg gggcacgacg ctgcttcccg ggcaggcgat cacgatggac
      601 cacgtcatgg actggacggc catcacctat ccgcagacca ccgtcaacga gttcaccggc
      661 gagatgatca agacgatcct cgaggacgtc tgcgacaacc tgttcaaccc cgacccgtac
      721 taccagcagg gcggcgacat ggtgcgcgtc ggcggcctca cctacacctg caccccgggc
      781 gagacgatgg gcaagcgcat ttccgacatg cggctggccg gcaagccgat cgccgccgac
      841 cgcaagtaca aggtggcggg ctgggctccc gtcgcggaag gtgccagggg cgagccgatc
      901 tgggacgtgg tcaccgcgta cctcaaggat cgcaagtcca tccctcccgt gaagctcaat
      961 ctcccgaagc tggtcggcat ggaaggcaac gccgggattg cctgagccgg tcgcgggaac
     1021 cccgagcccg ccaccgacac cgcgaaatac cctttgttcc cctcaagttg gcgcggttcg
     1081 cccggaagac ggacggattc gccaccacga aacccccggc cgacttgcgg cagatcaaca
     1141 gggggtaggc ccaagccccg taatgtcacc aaagtcctac aggatctgcc cgctccgggg
     1201 cctggggccc ctccggcgga cgcggttcca cgggcatccg caagccgaag aggggttcaa
     1261 aaatgcatct ggcccaagcg tcgtcctacg agatcgtcac tcgcgaggac ttttccgacg
     1321 tcacgttcct gctcgaagtc cggcatccga tgctggccaa ggccgccaag gccgggcagt
     1381 tcgtcatcgt gatggagcac gagcagggcg aacgcatccc gctcaccatc gccgacttcg
     1441 acccgaaggc gggcaccatc acgctggtca tccaggccgt gggcaagacc acccgtgaaa
     1501 tgcagcagaa ctgccgggtc ggaacaacgc tgcacgccgt cgtggggccg atgggcattc
     1561 ccagccacat cggatcgaag aagaaagtgg tctgcgtcgg cgggggcctg ggcgtcgcgc
     1621 cgatcttccc gcaggcacgg gcgtacaagg aaagcggcgc ctacgtgatc ggcgtcctgg
     1681 gcttccgcaa tcgcgggctg gtgttctggg aggacaagtt ccgcgcctgc tgcgacgagc
     1741 tgatcctgtg caccgatgac ggctccgccg gcatcaaggg actcgtcacc gagggcatca
     1801 agcaggcgat cgcgaagcac cccgacatcg aggaactggt ggccatcggg ccgccgatca
     1861 tgatgaaagg ctgcgcggag gcaacgcgcc cgcacaagat caagacgatg gtgagcctga
     1921 acccggtcat ggtcgacggc accgggatgt gcggcggatg ccgcgtgaag atcggcgaaa
     1981 ccatcaagtt cgcctgcgtg gacgggccgg acttcgatgc gcacctcgtc gacttcgacg
     2041 acctcatggc gcggctgggc cgctaccgcc ccgccgagaa acaggcgatg gaacgctggc
     2101 aggagagctg ccgcatccgg ggcgagggcg cccaggcacc cgagcccgcc gtcgcaatgg
     2161 acgaccgtcc ctcgaccgac ggctgacgca gccgcgtcgc cagggcacga gccggcacaa
     2221 gcaaccggaa ggaatggcaa acatggccaa gaagaaaacg attcgcacga tcccgcagga
     2281 acgaacgccc gccaaggagc aggatccgct cgtgcgcgcc cgcaacttcg aggaggtttc
     2341 gctgggctat cgcctggagg aggcgctcgt cgaggccgaa cgctgcctgc agtgcgccga
     2401 cgagccctgc gtgcgtggct gtcccgtggg catcgacatc ccgggcttca tccagaagat
     2461 cacggagaag cactaccacg gcgcctacga catcatcacc gacaccaacc tcctgccctc
     2521 ggtctgcggg cgcgtctgtc cgcaggagaa ccagtgcgaa ggtgtctgca cggtaggcga
     2581 cacgctcgag cccgtggcca tcggccgcct cgagcgcttc gtcggcgaca tggccatcaa
     2641 ggaaggatgg gccaacatcc cgtacatcga gccgaacggc taccgcgtcg gcatcgtggg
     2701 cgcgggtccc gcgggcatgg cctgcgcggc ggacatggcc aaggccggct gcgaagtcgt
     2761 ggtctacgag gccttccacc agcccggcgg cgtgctcaag tacggcatcc cggacttccg
     2821 cctgcccaac gcggtgatcg acgcggagat cgacaagctg cgcgccctgg gcatccgctt
     2881 cgagtgcaac accctggtgg gccgcctctt cacgatcgag cagatgatca ccgagatggg
     2941 cttccacgcg gtgttcctcg gcaccggcgc gggctacccg agcttcatgg gcattcccgg
     3001 cgaatcgctc aacggcgtgc tctccgccaa cgaactgctc acgcgcgcca acctgatgcg
     3061 cgcgcgcgat ttcccgaact tcgacacgcc gctgcagccc ggcaagtgcg tgacggtgat
     3121 cggggcgggc aacaccgcga tggatgcgat gcgcgtctcg atgcgtctgg gcgccgagaa
     3181 ggtctactgc gtctaccggc gcaccaaggc cgaggccccg gcgcgcgccg aggaagtcca
     3241 ccacgcggag gaggagggca tcgatttcaa ctggctcacc agccccatcg agctgctcgg
     3301 cgatgccagc aacgcggtgc gcgcgatgcg ctgcatccgc atggaactcg gcgagcccga
     3361 cgactccggc cggcggcgcc ccgtgcccgt gccgggcagc gagttcgaga tcgcgaccga
     3421 catggtggtc ttcgcgatcg gcaccaacgc caatcccatc ctcggccaga ccgcgaagct
     3481 caagctcaac aagcgcggct acatcgagac ggacgagacc ctcgcgacct cgatcgccgg
     3541 cgtctatgcc ggcggcgaca tcgtcacggg cgcggccacc gtgatccagg ccatgggcgc
     3601 cggacgccat gccgcgcgca gcatgaaggc ctacctgggc ctgcgcgatt ccgattgcgt
     3661 gtatgcgccc gacaccgacg acccgaccgg ccggcgcttc ggaatcccgg ccgaggaaca
     3721 caacttcgca cgggtgcggc tgctctagcc cggccggatc ccttcccttc gacgcacgac
     3781 tcccaaggcg aaaccatgag tgcaacggcc cagttggacc gaaagaagca ggcgcgaggc
     3841 aatgcagccc ccgccgcggg cgccaagccc gtcatcacgc tgtccgaaca catcgtcgag
     3901 gtgatcagcg attccggcga gggcgcgcag cgctgcggcc aggcgctggc cggtatcgcc
     3961 gcccgcagcg gcaacggcat ctggacggtc gagatcattc ccgccgagat ccagccgccg
     4021 gcgcgcagcg tcgccggcgc gagcggcatc cagatccgca tcggctcgaa gcaggtgacc
     4081 aacggcggcg acgagaccga cctggtcgtc gccttcaacg agcaggtcct cctcggacgc
     4141 gtgcgcgcag gggaactgaa gcccggcgcg acgatcctgc tcgagaacgc ctggcgtgaa
     4201 caccgcgacc cgtcgatcgt gaagtcgttc aacgacaccg tcgacgcgct cgtgaagggc
     4261 gggttccggt tgatcgagat cccgctggaa cgcgagtgcc gggccctcat gacagacgcg
     4321 cggcgcggca agaacatgtt cgtgctcggc atgctgtgca gcatctacag cctggacctg
     4381 cagctcgccc gcgaccagat cgcgctaacc ttcggcaaga aggaccgctc cgtcatcgac
     4441 accaacgtga agctgctcga ggcgggcttc gcgtggggcg aggcgaacct cgacttcaag
     4501 tacgagatcc cggcccagcg cagcacggtg gcgcaggtcg tcatcaacgg caacaccgcg
     4561 atcgcgctcg gcgtgctcgc ctccggcatg gacatctgcg cgatgtaccc gatcacgccg
     4621 gcgacctccg cctcccatta cctgtccgac tgcttcgaga aggtgggcgg catcgtccac
     4681 caggcggagg acgagatcgc cgcctgcgcc ttcgccatcg gcgcgtccta cgcaggccgg
     4741 tgcgcggtga cgatcacctc cggtccgggt tactccctca agcaggaagg catcggcctc
     4801 gccgtcatgg ccgagattcc gctggtcgtg gtgaacgtgc agcgcggcgg gccgagcacc
     4861 ggccagccga cgaagatgga gcagggcgac atgctcaccg ccgtctatgg aagccacggc
     4921 gacgcgccca aggtggtgat ggccgtgagc ggcatcgagg attgcttcta ctcgatgatc
     4981 accgcgagga agatcgcgga gaccttcaac atggtcgtcg tggtgctgtc ggacgcgagc
     5041 ctcgccacgg cccaggcgcc gttcccgcgg ccggtcttca gcgaagactg gctggcgccc
     5101 ccgctggacc agtcgcccat accggagggc gcccggccct acgactggga tcccgtcacg
     5161 ggcatcgcgc ggcgcttcgt gccgggccag ccgggcggca tgcacacggt gaccggcctc
     5221 gcccatgacc ggtcgagcaa ggtcgcctac gacccgtcga tcaacgagga atccctgcgc
     5281 gcccgcagcc tcaagctcgc ggcgctgcag aagacgctca cgaccccgcc cgtgttcggc
     5341 gaccccgagg gcgacctgct cgtcgtgggc tggggcagca ccaagggcgt gatcgaggag
     5401 gcggtggaac gcctgcgcgc cgagggcctc aaggtctcgt cgctgcacat gcgcttcatc
     5461 cagcccatgc catcgggcat cggcgagatc ctgaaacgct tccgcaaggt gatgaccgtc
     5521 gaggcggggt ggggcgaccg gctcgaggac gaactgatcg acgagaacaa ccgccgctac
     5581 acggcgctgg cgatgatgct ccgctcgcgc ttcctggtcg atgtcgactg cgtgagcgaa
     5641 gcgcgcggac agccgatcaa gcccggcaat gtggtgaagg cgctgcgcgc caaactcgag
     5701 gaaggcaaat agccatgggg aacgcactct cgcaatgcat catcaggctc cacgaggagc
     5761 agcacgacct cgaggactac cagggcggcg tgccgcgctg gtgcaacggc tgcggcgaca
     5821 acgcgatcct cgcggcggtg cagaagctgt gccgggacga gaacctggcg cccgagaaga
     5881 cggtgttcgt ctccggcatc ggctgctcga gccgcttccc gcactacatg aagacctacg
     5941 ggttccacgg catccatggc cgcgcgttcc cgatcgccga gggcgtgaag atggcccgcc
     6001 cggacctcaa cgtgttcgtg aacacgggcg acggcgactg ctgcagcatc ggcgccgcgc
     6061 actggatcca cgcgatccgc tacaacatga acctcaccgt gatcctgcac gacaaccacg
     6121 tctacgggct cacgaagaag caggcctcgc cgacctcgcc gatcgggttc aagagcaaca
     6181 ccacgccgcg cggctcgctc ctcgaggcgc tcaatcccct gaccgtgacg ctcggcgtgc
     6241 agaacgtctc gttcgtggcg caggccgtgg actggatgcc ggaagtcctc tcggacatcg
     6301 tctcgaaggc cttccaccac cgcggcttct cgttcatccg gatcgtccag cgctgccccg
     6361 agttcctgcc caagatgttc gagccgtggc tgcacgaccc gggcaagacg ctcctcctca
     6421 cccatcccaa cggcctgcag ccgagcgcgg acctggggcg gatctacaag aaccagcgcg
     6481 agcacgatcc gctggacatc aaccgcgccc gggaaatcgc ctcgctggag gatccgatcc
     6541 cggtgggcat cctctaccag aacccgcaag tgccgtgcta cgaggacctg cgcggcgccg
     6601 ggaagcccag gacggtggat gcgatccgca agggactcga catcgagctc gacaagttca
     6661 ccatctggcc cgacgacgag agccagcagc gcgccgccta ggcgcacgac gatacaacga
     6721 ccgttgaggc aagttccgat ggacatggca gcgaaattcc aggaccagtt ggtcttccac
     6781 atgacgggcc ggcggacggg cgaggacctc gcccccgtcg acaccggatc gatgcgtccc
     6841 gcgctactcg ccggataccg ggacctcacg cgcctgcgcc acgactatcc gctggtgctg
     6901 gtggagagcg gcgacgtgga cggctttgcc gcgtcgctca cgctcctcgt caaccgcgtg
     6961 ctcgaaacgc tcgcgccgca cggcatcgag ggcgaacgcc tgcgcaagca cgtcctgcgc
     7021 ctcgagcacg agctgcgcgc catgctggcc acgggcgcgc acggatcgct cgcggatctc
     7081 tggacgaagg cagccggaag gctcgcctcc gacgccgatg aaagcgtggc gaagatcctc
     7141 gaggccgcgg gcgaggcgat cgggatcgac ggcgagctgg tcgactgcga cgagagcctg
     7201 ccgtcgcgct tcatggtcca cgcctggcgt cacgtgcagg cgcagaaggc ggcactgttc
     7261 cacggccacc tcgacaacct gcagaggaag ctctcggaca tcctgcgggc ggccttcgcg
     7321 cactccgagg cgggccagca gcccgaagcc ctgcgcgctt ccttcggcgg aacacaggcg
     7381 aacgacttcg atttcgccga gatgtcgcgc cttgtcgtgc gcggcgctcc caaggacgaa
     7441 ttgccggcag cccggcgcaa gcgaatcgag tgggcccttt cggtcctcaa gtcgcagcgc
     7501 ttccacgcct cgcggacggt tgcggccggc gacgacgcgc cctacgagtt cctgttcgac
     7561 aacatcgcgg cggccgtggc ggcctaccgc gagcggcttg cgcaggcggt cgagctcctg
     7621 aaggcgatcg cgatcgcgga gctcgaaacg cgcaacggct atgacgaggc ggaccacgac
     7681 cagttcttcg aggcctacga caagcgcgcc ctcacggcag acgacctggc gctgttcccc
     7741 gactacctgg tgtgcattcc tgccgagcgc aacagcgcgc cggaaaacgc ggccctgatg
     7801 gacacgttgt cctccgggct gccggtcaag gtggtcgtgc aggtcacgga cctgctcgag
     7861 gaggcctcca tcggcaccgg ccactatgcc ttcggcgtgc gcagcgcccg cctggcgacc
     7921 acggcgatgg ggcttggcgg gatgttcgtc ctgcaggccg ccagttccag cctgtaccgg
     7981 atgcgcaacc ggatcgaggg cggcatgggt tgccggggac cggctctctt cagcgtgttc
     8041 tccggtgcgc cggctgcggc gggcgagatg ccgcgctatc tcacggcggc ggcggccatg
     8101 gagtcgcgcg cctttccggc gttcgtctat gacgccgcgg gcggggacaa ctgggccacg
     8161 cgattctcgc tggagcacaa ccgcaacccc gacaccgact ggccggtcga aaccttcgag
     8221 tacgccgatg gcacgatgca gcgggtgcgc gaggaagtcg ccttcaccta tgccgacttc
     8281 gccttgtgcg accggcgcaa cgccgagcac ttcgccgtcg tttcgcgcga gcgctggacg
     8341 gccgccatgg tcccggcggg ggattggctg gcgctgacgg agcgcgacgc atccgaccgc
     8401 attccctacc tgctggccat tgacgcgcag gacgtcctgc accgggtcgt cgtcgacatc
     8461 aggatgatgc aggccacgcg acgctgcctg ctgctgtggc accgcctcca ggagcacggc
     8521 ggcatccacg attcgcacgc cgagcgcctg ctcgcgcgcg agaaggccgc gtgggaagaa
     8581 acgcacaagc gcgatctcca agcggcggcg gcgaatgccg tgccggccga ggtggcgccc
     8641 gcggcgacga gcgcaa
//
LOCUS       DASUEV010000223         6996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_990968, whole genome shotgun sequence.
ACCESSION   DASUEV010000223 DASUEV010000000
VERSION     DASUEV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990968"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="VFV55_07210"
     CDS             complement(<1..1819)
                     /locus_tag="VFV55_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX4944126.1"
                     /translation="MSHRLDSLLRPRSIAVLGATERVGTVGRNTVENLLKGGFRGPLF
                     AVNPGYATVCSVPCFPSLAALPERVEHVIFAIGDARIEAALDDVIDHGARAVTLMSSL
                     ILAEDREPLLRDRVAAKLRAASLLACGANGMGFYNFADGIWGCGFRTRTHARGGNVAY
                     ISHSGSGMCGIVDSEERIDFNLVVSTGQELIVAMDEYLDFALEQPGTRVVGLFMETAR
                     NPEGLIRGFEKARQRGIPIVALKVGRTELAARLAVSHSGAIAGEDGVYQALFDRHGVQ
                     RVRDMDELATALIMFAQPHPVHAGGLVSLHDSGGERQLLIDLAHDAGIPLTQLSAATT
                     SRLTALLDPGLPPINPLDAWSTGGPDYHVAMEQCFAALMCDPGAAIGAVIHDRIAGGG
                     IQDSYLDYLRAGHRASGKPAFLVANRQGTGADERVVAATREGFPVLDGVPQFLQGARC
                     LFEYRDFLARKRDPADQLPGSVLTKWRSRLATGRALDEHDAMKLLSDFGLPANPCRVV
                     EDEPSARVAARECGFPVALKTAMHGIEHKSDRGGVHLGVADEAALSVAYRDLANRLGP
                     RVLVAPMVRSPGVEMLLGMVHDDQFGPVVVLGAGGLHVEA"
     gene            complement(1844..3052)
                     /locus_tag="VFV55_07215"
     CDS             complement(1844..3052)
                     /locus_tag="VFV55_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX4944127.1"
                     /translation="MSIKDDMNRKEHFRRAVWETPFHPRTSALNTLNEWHRWKDYTTA
                     DAYFDEALEYAAIRNACAVFDLTPMTKHLITGPDALAFLNRLVTRDVAKIKPGKVGYA
                     IWCDDNGQVIDDGTIFHLREGAYRLCSQERQLDWLTRSAFGFDVTIVEDTHDVAALAL
                     QGPTSCAVLKAIGCAGVQNLKPFDIAYYPFAGGELMVSRTGFTGDLGYELWIDPPRAL
                     DLWDALFDKGKNHGIRPMGTHALEVARIEAGFIQAQVDFHPADQAIMLDRSRSPFELD
                     LGRLVDFSKPNFNGRRALLQEKKNGSRFRFVKLDIEGNKPARSAYVYDKDKNVVGTVT
                     SAEWSPSAKANIAYASMKMPWGRPGDELWAEIYYQRELKWSRVMARCRVVEGAFFDPP
                     RRRATPAADF"
     gene            complement(3049..4614)
                     /locus_tag="VFV55_07220"
     CDS             complement(3049..4614)
                     /locus_tag="VFV55_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4944128.1"
                     /translation="MTDRNRIAIVGGGHNGLVCAAYLARAGREVVVFEAAAQVGGASV
                     TREFTKGFRVSACAHICYQLDSGIVRELKLASHGLKWAHKGLKTVALAHAGEHLVLDG
                     GKLVSGPLSGGDRQGLADYHARMLRFARVLAKQHNRLPPRLGGGVRSDMSGAVKLGLD
                     IRLLGRESMREFLRIAGINIYDVLEEIFESPLLKGALALDAVLGANLGPRSNNSVLAL
                     LHRLSGQCQGNGGFSIPEGGMGAISEAIAAAARAAGATIRTGSPVARITLEGDAVSCV
                     ELESGEKIPAATVVSNADPATTLLRLVGARHLETGFVHRVRHFRSKGMAAKLHLALDA
                     LPAFARLPVELAGERLVISPDLVHLEHAFDHAKYGQCSPDPALEITIPSVHDKTLAPA
                     GKHVLSAIVQYAPFDPRASGDGPRRELLERTLGVLERHAPGLRRQIVASELLLPADIE
                     REFRITGGHWHHGELALDQFMMMRPVPGAAQYAMPVGGLFLCGAGCHPGGGVMGSAGR
                     NAANAVLATERAA"
     gene            complement(4607..6223)
                     /locus_tag="VFV55_07225"
     CDS             complement(4607..6223)
                     /locus_tag="VFV55_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX4944129.1"
                     /translation="MAKYDAIVVGAGHNGLTNAAYLARSGLKVLVVERNPWIGGAAVS
                     REIEKGWIYSNCSYVCSLLRPELFRDLELARHGLQVVPYGGGVSITSKGDILGNYTDE
                     QVQRREFARHNPRDADAYHRYSTDVMRQCKFIRPLLLRTPPDPTSFKPRDLREMIFLG
                     RELSKFGEQFIYDTLRFYSMSIADFLNEYFESDAIKCHLSGSGIIGSAMGVYSPGTAY
                     VLLHHYMGDVDGSIGAWGFARGGMGAVSKAIASSFKSFGGEIVTDAPVARVIVKGGRV
                     AGVVTENGTEYHADIVVSNLDPRRTYTKIFDPTDLPPAIVEKARNWKIRGSSGKLNIA
                     LDGLPEFPALGEDNPLAYGDMHFLDSLEKMERAYDDWKNGTWSKEPYLDLLIPSMTDP
                     TMAPPGKHFMSVFVQYVPPFIHGREWTDADREGFKNTVLDQIARYSPNFKSLVRHVEC
                     RTPRELENEVGLTEGNIFQGELTMDQLLFNRPFPGYAQYRGPVKGFYMCGSGTHPGGG
                     VMAAPGANAAREILMDLRRPNIVPEGWSDD"
     gene            complement(6238..>6996)
                     /locus_tag="VFV55_07230"
     CDS             complement(6238..>6996)
                     /locus_tag="VFV55_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4944130.1"
                     /translation="SVFKAYGDQIAAFMIEPIMGNCCSISATREFLRDARVLCDKYGV
                     LLIVDEVKTGFRVARGGVQELFGVKADLCTFAKAMGNGYPISVLCGREEIMRKFGNGV
                     VHGGTFTCHSVALAAAEKTLEILDETPALAKIADYGTKLRGGMSQILAQRGIKHSFSG
                     HPSMSGLFFSEKPPRDYRDWFTSDYTFYEALAPHLHDLGVLCEPDSREPWFICEAHDE
                     RCLGETLNRFETAVDQVVRSAPRPASAPFTKYTT"
BASE COUNT         1174 a         2304 c         2353 g         1165 t
ORIGIN      
        1 gcgcctcgac atgcaggccg ccggcgccga gcaccacgac gggtccgaat tgatcgtcgt
       61 gcaccatgcc gagcagcatc tcgacgcccg gagacctgac catcggcgcg acgaggacac
      121 gcgggccgag ccgattcgcc aggtcccggt atgcgaccga gagcgcggcc tcgtccgcca
      181 cgccgagatg cacaccgccg cgatccgact tgtgctcgat gccgtgcatc gccgtcttca
      241 gcgcgaccgg gaagccgcac tcgcgcgcgg cgacgcgtgc ggacggttcg tcctcgacga
      301 cgcggcaagg attcgccggc agcccgaaat cgctcagcag cttcatcgcg tcatgctcgt
      361 ccagtgcgcg gccggttgcg agccgcgagc gccacttggt gagtacggag cctggaagct
      421 gatctgccgg atcccgcttc cgcgccagaa aatcacgata ctcgaacagg cacctcgccc
      481 cttgcaggaa ctgcgggaca ccgtcgagca ccggaaagcc ttcgcgcgtc gccgcgacca
      541 cgcgttcatc cgcgcctgtg ccctgacgat tggcaacgag gaacgccggt tttccggacg
      601 cccgatgacc ggcccgcaga tagtcgaggt agctgtcctg gatcccgcca ccggcgatgc
      661 gatcgtggat cacggcgccg atggccgctc ccgggtcgca catcaatgcc gcgaagcact
      721 gttccatggc gacatggtaa tccggcccgc cggtactcca cgcatcaagc ggattgatcg
      781 gcggcagacc cgggtccagc aacgccgtga gccgggaagt cgtcgcggca gacagctgcg
      841 tcagaggaat tcctgcgtcg tgtgcgaggt cgatcagcaa ctggcgttcg ccgccggagt
      901 cgtgcaacga gacgaggccg ccagcgtgga ctggatgcgg ctgggcgaac atgatcagcg
      961 ccgtggccag ttcgtccatg tcacggacgc gctgaacgcc gtggcgatcg aacagcgcct
     1021 ggtagacgcc gtcctcgccg gcgattgcgc ccgaatgcga taccgcgagc cgggcggcaa
     1081 gctcggtgcg ccctaccttc agcgcgacga tcggaatgcc acgctggcgc gccttctcga
     1141 atccgcggat caggccctcg ggattgcgcg cggtttccat gaagagtccg acgacgcgcg
     1201 tccccggctg ttcgagcgcg aaatcgaggt actcatccat cgcgacgatc agttcctggc
     1261 cggtcgacac caccaggttg aagtcgatgc gctcctcgct gtccacgatg ccgcacatgc
     1321 ccgagcccga atggctgata taggcgacat tcccgccgcg ggcatgcgtg cgggtcctga
     1381 agccgcaacc ccagatgccg tccgcgaagt tgtagaaacc catgccattc gcgccgcagg
     1441 caagcagact agccgcgcgc agcttggccg ccacgcggtc gcgcagcagc ggctcccggt
     1501 cctctgcgag aatcagcgag gacatcaggg tcaccgcgcg cgcgccgtga tcgatgacgt
     1561 cgtcgagcgc cgcctcgatg cgtgcgtcac cgatggcaaa gatcacgtgc tcgacccgct
     1621 ccggcagggc cgccagggac ggaaaacagg gcacgctgca gaccgtagcg tatccgggat
     1681 tgaccgcgaa cagcgggccc cggaatccgc ccttcagcag gttttcgacg gtgttacggc
     1741 cgacggtgcc gacgcgctcg gttgcgccca gcacggcgat cgagcgcggt cggagcagcg
     1801 aatcgagccg atgagacatg gcgggcccgc gtccgcgccg cggtcagaag tccgctgcgg
     1861 gcgtcgcgcg gcggcgcggc gggtcgaaga atgcgccttc aacgacgcgg cagcgcgcca
     1921 tcacgcgcga ccacttgagc tcgcgctgat agtagatctc ggcccacagt tcgtcgccgg
     1981 gccgtcccca gggcatcttc atggacgcgt aggcgatatt ggccttcgcg gaaggcgacc
     2041 actccgccga cgtcaccgta ccgaccacgt tcttgtcctt gtcatagaca taagccgagc
     2101 gcgccggctt gttgccctcg atgtcgagtt tcacgaagcg gaatcgcgag ccgttcttct
     2161 tctcctggag cagggcacgg cggccgttga agttgggctt gctgaaatcc acgagccgcc
     2221 caaggtcgag ctcgaagggc gagcgcgaac ggtcgagcat gatcgcctgg tcggccgggt
     2281 ggaagtcgac ctgcgcctgt atgaagcctg cctcgatgcg cgcgacctcg agtgcgtgcg
     2341 tgcccatcgg gcggatcccg tgattctttc ctttgtcgaa gagcgcatcc cacaggtcga
     2401 gcgcgcgcgg cgggtcgatc cacagttcgt agccgagatc gcccgtgaat ccggtgcgcg
     2461 acaccatcag ctcgccgccg gcgaacgggt aataggcgat gtcgaaaggc ttgaggttct
     2521 gcacgccggc gcagccgatc gccttcaaca cggcgcagct ggtcggcccc tgcagcgcga
     2581 gcgcggcgac gtcgtgcgta tcctcgacga tggtgacgtc gaagccgaag gcagagcgcg
     2641 tgagccagtc gagctggcgt tcctgagagc agagccgata ggcgccctcg cgcaggtgga
     2701 agatcgtgcc gtcgtcgatg acctggccgt tgtcatcgca ccagatcgcg taaccgacct
     2761 tgccgggctt gatcttggcg acatcgcgcg tgacgaggcg gttcaggaag gcgagggcgt
     2821 cgggacccgt gatcaggtgc ttggtcatcg gcgtcaggtc gaacacggcg caggcattgc
     2881 ggatcgccgc gtactcgagg gcctcgtcga aataggcgtc ggccgtcgtg tagtccttcc
     2941 agcggtgcca ttcgttcaga gtgttgagcg ccgaagtgcg cgggtggaac ggcgtctccc
     3001 acacggccct gcggaagtgc tccttgcggt tcatgtcgtc cttgatgctc atgccgcgcg
     3061 ctccgtggcg agtaccgcat tcgcggcatt gcggccggcg gaacccatca cgccgccgcc
     3121 cggatggcag cccgcgccgc acaggaagag cccgccgacc ggcatcgcgt actgcgccgc
     3181 tcccggcacc gggcgcatca tcatgaactg gtcgagcgca agctcgccgt gatgccagtg
     3241 tccgcccgtg atgcggaact cccgctcgat gtccgcgggc agcagcaatt cggaggcgac
     3301 gatctgccgg cgcaggcccg gcgcgtggcg ctcgagcacg ccgagcgtgc gctccaggag
     3361 ctcgcgccgc ggcccgtcgc cgctcgcgcg cgggtcgaag ggcgcgtact ggacgatcgc
     3421 cgacagcacg tgcttgcccg ccggcgcgag cgtcttgtcg tgcaccgagg gaatggtgat
     3481 ctccagcgcg ggatcggggg agcactggcc gtactttgcg tgatcgaagg cgtgctcgag
     3541 gtggacgagg tccggcgaga tcacgagccg ctcgcccgcc aattcgacgg gcagccgggc
     3601 gaatgcgggc agcgcgtcga gagcgaggtg cagcttcgct gccatgccct tgctgcggaa
     3661 gtggcgtacg cgatggacga agcccgtttc gaggtgtctg gcgcccacca gccggagtag
     3721 cgttgtggca ggatctgcgt tcgagacgac cgtcgcggcg ggaatcttct cgccgctctc
     3781 gagctcgacg caggagacag catcaccttc gagcgtgatg cgcgcgaccg ggctgcccgt
     3841 gcggatcgtg gcgccggcgg cgcgcgccgc cgccgctatc gcctcgctga tcgcgcccat
     3901 gccgccttcc ggaattgaga acccgccgtt gccctggcac tgtccgctca ggcggtgcag
     3961 cagcgcgagc acggaattat tcgagcgcgg gccgaggttc gcgccgagca cggcgtcgag
     4021 cgcgagcgcc cccttcaaga gcggcgactc gaaaatttcc tcgagcacgt catagatgtt
     4081 gatgccggcg atccgcagga actcccgcat cgattcgcgg ccgagcagcc ggatgtcgag
     4141 gccgagcttt accgcgccgg acatgtcgga gcgaacgccg ccgccgaggc gcggcggcag
     4201 gcggttgtgc tgcttcgcaa gcacgcgcgc gaagcgcagc atccgtgcgt gatagtcggc
     4261 gagtccctgg cggtcgccgc cggaaagcgg gccggacacc agttttccgc cgtccagcac
     4321 gagatgctcg cctgcgtggg ccagcgcgac ggtcttcagc cccttgtgcg cccacttcag
     4381 gccgtgcgaa gccagcttga gctcccgaac gatgccactg tccagttgat aacaaatatg
     4441 agcgcaggca gagaccctga atcccttcgt gaattcgcgc gtgaccgaag cgccgccgac
     4501 ctgggctgct gcctcgaaca cgacgacctc gcgccccgca cgggcgaggt aggcggcgca
     4561 gacgaggccg ttgtggccgc cgccgacgat ggcgatgcgg ttgcggtcag tcatcgctcc
     4621 agccctccgg aacgatgttc gggcgccgca ggtccatcag gatctcgcgc gcggcattcg
     4681 cgccgggcgc cgccatcacg ccgccgcccg ggtgcgtgcc ggatccgcac atgtagaaac
     4741 ccttcacggg cccgcggtac tgcgcatagc cggggaacgg cctgttgaac agcagctggt
     4801 ccatcgtcag ctcgccctgg aagatgtttc cctcggtgag cccgacttcg ttctcgagct
     4861 cgcggggtgt gcggcactcc acatggcgga cgagcgactt gaagttcggg ctgtagcggg
     4921 cgatctggtc gagcaccgtg ttcttgaagc cttcccggtc cgcgtcggtc cactcgcggc
     4981 cgtggatgaa cggcggcacg tactgcacga acaccgacat gaaatgcttg cccggcggcg
     5041 ccatggtcgg atccgtcatt gacgggatca gcaggtcgag gtatggctcc ttcgaccagg
     5101 tgccgttctt ccagtcgtcg taggcgcgct ccatcttctc gagcgaatcg agaaagtgca
     5161 tgtcgccgta ggcgaggggg ttgtcctcgc cgagcgccgg gaattccggc agtccgtcga
     5221 gcgcgatatt gagttttccg gaggagccgc ggatcttcca gttgcgggcc ttctcgacga
     5281 tcgcgggcgg cagatccgtg ggatcgaaga tcttcgtgta ggttcgccgc gggtcgaggt
     5341 tcgagacgac gatgtcggcg tggtactcgg tgccgttctc cgtcacgacg ccggcgacgc
     5401 ggccgccctt gacgatcacg cgcgcaaccg gtgcatccgt gacgatctcg ccgccaaagg
     5461 acttgaacga gctcgcaatc gctttcgaca ccgcgcccat tccgccgcgc gcgaatcccc
     5521 aggcgccgat cgagccatcc acgtcgccca tgtaatggtg caggagcaca taggccgtgc
     5581 cgggcgagta gacgcccatg gccgaaccga tgatgccgct gccggagagg tggcacttga
     5641 tggcgtccga ctcgaagtac tcgttcagga aatccgcgat gctcatcgaa tagaaacgca
     5701 gcgtgtcgta gatgaactgc tcgccgaact tgctgagctc acgaccgagg aaaatcatct
     5761 cgcggaggtc gcgcggcttg aacgaggtcg ggtccggcgg cgtgcgcagc agcagcgggc
     5821 ggatgaactt gcactggcgc atgacgtcgg tcgagtagcg gtggtacgcg tccgcgtcgc
     5881 gcgggttgtg gcgcgcgaac tcgcgccgct gcacttgctc atcggtgtaa ttgccgagaa
     5941 tgtcgccctt cgaggtgatc gacacgccgc cgccgtaggg cacgacctgc aggccgtggc
     6001 gcgcgagctc gaggtcgcga aagagctccg gccgcagcag gctgcagacg taggagcagt
     6061 tcgagtagat ccagcccttc tcgatctcgc ggctgacggc agctccgccg atccagggat
     6121 tgcgctcgac gaccaggacc ttgagtccgg aacgtgcgag ataggccgcg ttcgtcagac
     6181 cgttgtggcc cgcgccgacg acgatcgcat cgtatttcgc caaggtcggt tctttcttca
     6241 ggtcgtgtat ttcgtgaatg gcgcgctggc cggcctcggt gcgcttcgca cgacctggtc
     6301 cacggcggtc tcaaaacggt tcagcgtctc ccccaggcag cgctcgtcgt gcgcctcgca
     6361 gatgaaccag ggctcgcgcg aatccggttc gcaaagcacg ccgaggtcgt gcaggtgcgg
     6421 cgccagtgcc tcgtagaaag tgtaatcgct cgtgaaccag tcgcggtagt cacgcggcgg
     6481 cttttcgctg aagaacaggc cgctcatgga cgggtgcccg ctgaacgaat gcttgattcc
     6541 ccgctgcgcg aggatctggc tcatgccgcc gcgcagcttc gtgccgtagt cggcgatctt
     6601 cgcgagggcg ggggtctcgt ccaggatctc gagcgtcttc tcggctgccg cgagcgcaac
     6661 ggaatggcag gtaaaggtgc cgccgtgcac gacgccattg ccgaacttgc gcatgatttc
     6721 ctcgcgtccg cacagcaccg agatcgggta gccgttgccc atcgccttgg cgaaggtgca
     6781 gaggtcagcc ttgacgccga agagctcctg caccccgccg cgcgcgacgc ggaatccagt
     6841 cttcacctcg tcgacgatca gcaggactcc gtatttgtcg cacaacacgc gggcgtcacg
     6901 caggaactcg cgcgttgccg agatcgagca gcaattgccc atgatcggct cgatcatgaa
     6961 cgcggcgatc tgatcgccat aggccttgaa cacgct
//
LOCUS       DASUEV010000224         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_394699, whole genome shotgun sequence.
ACCESSION   DASUEV010000224 DASUEV010000000
VERSION     DASUEV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394699"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="VFV55_07235"
     CDS             complement(<1..1513)
                     /locus_tag="VFV55_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944131.1"
                     /translation="MADRHLLYLTNENLVSLVARGERIATRTVFPVSGTGKDDFERYI
                     RSVRTLPTHVITDLAEEDFRIDTIPHLGGRDREAVLTRKLNQLFRNTPYRHAMAQGRE
                     VEGRRDDRVVYTAITNGEILRPWVEVLERLQVPVEGICSSAVFSGTLLAELHLVFPHT
                     LLVTFTPGDALRQTYFRNGEIKFSRLTPVDLAEGETLGALLSGEIARTWQYLDSQRNF
                     SPADRLEVCVLIHPKDRPAVEPTLRDYDQIEYRLLDIEQVAATLGLKPPPVSSSAEEI
                     LAHLFLRKPAENHFASPELRRYAVLRNARNAIGATAGVILAAGIAFGGWNSYSALQNH
                     DREQETLRRVLASQRELGEIMKSMPTQGADGDTMRDTVLFYSGSLRGYPTVASMLLPV
                     SHVLERYPGIRLTQVAWQAADDDKASPAMVPISPREVPPLTAIVKGAGATPAAGQQVP
                     AGTADAAFPSGRHAVALLEGTVTVQGLGFREALAQVEALVADMGRIPGYQASLV"
     gene            complement(1520..2722)
                     /locus_tag="VFV55_07240"
     CDS             complement(1520..2722)
                     /locus_tag="VFV55_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX4944132.1"
                     /translation="MALFAYRAVDEQGRISSGSLDATNAIDLEMRLRRLGLDLVTFES
                     VKRSTAARSRSVTRPELITFCFHLSQLLRAGVNIIEALTDLRDTVDNAGFRQVVAGLI
                     EDIEGGLRLSDAMAGHPYIFDAVFVALVRAGEQSGQLNEVLDELSENLKWQDEMASQA
                     QRALVYPAIVAVVVVGVIFVLMIFLVPQLAITFKALVPKLPRETELLIALSNVFVKYW
                     YLLIGVPAAAVAAIVIAARTNEGLQDRLASISITLPVLGPIRFKIMLARFSTFFAMLY
                     RSGISVLDCIQICEKIMGNRLMEQALQRVGRSISEGQGISQAFTATKLFPPLVLRMLR
                     VGESTGGLDAALLNVSYFYNREVREGIAKMQALIGPATTIVLGGLIVAILYTIFLPLY
                     DVISKIKF"
     gene            complement(2731..>3061)
                     /locus_tag="VFV55_07245"
     CDS             complement(2731..>3061)
                     /locus_tag="VFV55_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HEX4944133.1"
                     /translation="CAVAYEPDPATRKLLAMDASDDRPVYWSVGCPKCENQGYKGRLA
                     IMEIFKLDSEIDELIARRATGREIRTAASAKGFRTLADDAISRVLLGQTSLEEISRVV
                     DLTDRVI"
BASE COUNT          499 a          982 c         1075 g          505 t
ORIGIN      
        1 ccacgaggct cgcctggtaa cccgggatcc tccccatgtc ggcgacgagg gcttcgacct
       61 gtgccagcgc ctctcggaat cccagcccct gaactgtcac ggtcccttcg aggagggcga
      121 ccgcatggcg tcccgatgga aatgcggcgt ccgcggtgcc cgcaggcacc tgttgccctg
      181 ccgccggagt cgcgccagcg cctttcacga ttgccgtgag gggcggcact tcccggggcg
      241 atatcggaac catcgcgggt gacgccttgt cgtcgtccgc cgcctgccac gccacctgcg
      301 tgaggcggat ccccgggtag cgttcgagca cgtgggaaac cggaagaagc attgacgcga
      361 ccgtcggata tccgcgcagc gatcccgaat agaagaggac cgtgtcacgc atcgtgtccc
      421 cgtccgcgcc ctgggtaggc atggatttca tgatctcgcc gagttcgcgc tgcgacgcga
      481 ggactcgccg cagggtctcc tgttcccgat catggttctg gagcgcggaa taggagttcc
      541 atccgccaaa ggcgataccc gccgcgagga tgactccggc cgtcgcgccg atcgcgttcc
      601 gcgcgttgcg caggaccgcg tagcggcgaa gctcgggcga ggcgaagtgg ttttccgcgg
      661 gcttgcgcag gaagaggtgc gcgaggattt cctcggcgct cgaggatacc ggcggcggct
      721 tgaggcccag ggtggcggcc acctgctcga tgtcgagcag gcggtattcg atctggtcgt
      781 agtcgcgcag cgtcggctcc accgcggggc ggtccttcgg gtggatgagc acgcagacct
      841 cgagccggtc cgccggggag aagttgcgct ggctgtccag gtactgccag gttctcgcga
      901 tctccccgga gagcagcgcc ccaagcgtct cgccttccgc gaggtccacg ggcgtgagcc
      961 ggctgaactt gatttcgccg ttgcggaagt acgtctggcg caatgcgtcg ccgggcgtga
     1021 aagtcacgag cagcgtgtgc gggaagacca ggtgaagctc ggcaaggagc gtgccgctga
     1081 aaaccgcgga ggagcagatt ccctcgacgg gtacctgcag gcgctccagc acttccaccc
     1141 acggccgcag gatctcgccg ttggtgatgg cggtgtacac cacgcgatcg tcgcggcggc
     1201 cctcgacttc gcgcccctgc gccatggcgt gccggtacgg ggtgttgcgg aagagctggt
     1261 tgagcttgcg ggtgagcact gcctcccggt cgcgaccgcc caggtgcggg atcgtgtcga
     1321 tgcggaagtc ctcctccgcc aggtcggtga tcacgtgcgt cggcaacgtg cgcacgctcc
     1381 tgatgtaacg ctcgaagtcg tccttgccgg tgcccgacac ggggaacacc gtgcgcgtcg
     1441 cgatgcgctc cccgcgggcg acaaggctca cgaggttctc gttggtcagg tagaggaggt
     1501 gccggtcggc catggggtgc tagaacttga tcttgctgat cacgtcgtaa agcggcagga
     1561 agatagtgta gaggatcgcc acgatcaatc caccgagcac gatggtcgtc gccgggccga
     1621 tgagggcctg catcttggcg atgccctcgc gcacctccct gttgtagaag tagctcacgt
     1681 tgagcagcgc cgcgtccagc ccgcccgtcg attcgcccac gcgaagcatt ctgaggacta
     1741 gcggcgggaa aagcttcgtg gccgtgaacg cctggctgat cccctggccc tcggaaatgg
     1801 accggccgac gcgctgcagc gcctgctcca tcagccggtt gcccatgatc ttctcgcaga
     1861 tctggatgca gtcgaggacg ctgatgccgg accggtagag catggcgaag aacgtcgaga
     1921 agcgcgccag catgatcttg aagcggatgg ggccgagaac aggcagcgtg atcgatatcg
     1981 aggcgaggcg gtcctgcagg ccctcgttcg tgcgcgcggc gatgacgatc gcggcaacgg
     2041 ccgcggccgg caccccgatc aggaggtacc agtacttcac gaacacgttg gagagggcga
     2101 tgagcagttc cgtctcgcgg ggcagcttgg gcaccagcgc cttgaacgtg atggcgagct
     2161 gcgggacgag gaagatcatc aggacgaaga tcacgcccac gacgaccacc gccacgatcg
     2221 ccgggtagac cagcgcgcgc tgcgcctggc tcgccatctc gtcctgccac ttgaggttct
     2281 cggagagctc gtccagcacc tcgttcagct gcccgctctg ctcgccggcg cgcaccagcg
     2341 ccacgaatac cgcgtcgaag atgtacgggt ggcccgccat ggcgtcggag agtcgaaggc
     2401 cgccctcgat gtcctcgatg aggccggcca ccacctgccg gaagcccgcg ttgtccacgg
     2461 tgtcgcgcag gtcggtgagt gcctcgatga tgttcacgcc cgcgcgcagg agctgcgaca
     2521 ggtggaagca gaacgtgatg agctccgggc gcgtgacgct gcgcgagcgc gccgcggtcg
     2581 accgcttcac cgactcgaag gtgacgagat cgagccccag gcggcggagc cgcatctcga
     2641 ggtcgatggc gttggtggcg tcgaggcttc cggaggagat ccggccctgc tcgtcgacag
     2701 cgcggtaggc gaagagcgcc acggccgccc ctagatgacg cggtcggtga ggtcgaccac
     2761 ccgcgagatc tcctcgaggc tcgtctggcc caggagcacg cgggagatgg cgtcgtcggc
     2821 gagcgtgcgg aagcccttgg cggaggcggc ggtgcggatc tcgcggcccg tcgcccggcg
     2881 tgcgatgagc tcgtcgatct cggaatccag cttgaagatc tccatgatcg cgaggcgccc
     2941 cttgtagccc tggttctcgc acttggggca ccccaccgac cagtagacgg gccggtcgtc
     3001 ggacgcgtcc atggcgagga gcttgcgcgt ggccggatcc ggctcgtagg cgacggcgca
     3061 g
//
LOCUS       DASUEV010000225         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1563866, whole genome shotgun sequence.
ACCESSION   DASUEV010000225 DASUEV010000000
VERSION     DASUEV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563866"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFV55_07250"
     CDS             complement(<1..479)
                     /locus_tag="VFV55_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4944134.1"
                     /translation="MTKARPRLLVVDDDRVVLSSMLAGLSRAGFDVVVATTGEEAMRV
                     AVDTHPALAVLDITMAGKTGLELAQLLSRETRVPFLLLAPLRDEADVRRKAATFGALG
                     VVAKPVDLTRLVPAVRTALERASSVLRVGEGADLLADALGGGHGPQALIALGILVER"
     gene            505..885
                     /locus_tag="VFV55_07255"
     CDS             505..885
                     /locus_tag="VFV55_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944135.1"
                     /translation="MRAPEIALNALRLLPVVLALGVLAAHFYRALAWVPFGITVALLP
                     LLFIRAPWAARVLQAALVAGALEWIRTAAGLVALRQSMGQPYTRLAIILGAVAVATGL
                     CALIFQWRPVRARFGMAEAKGPGA"
     gene            complement(882..2303)
                     /locus_tag="VFV55_07260"
     CDS             complement(882..2303)
                     /locus_tag="VFV55_07260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4944136.1"
                     /translation="MLALLSLLVGQSIYTSRQQHEEQGVVETYSVARVLESHVSGLVG
                     NVKLALHTVATEQERRLAGVGSDEQEGRAYIADIHANLPDVVGIRITDATGQMVYQTE
                     FADSPAPGVEDQPYFAMLRDEHSSSIAISGPHHNGPGGPWVIDIAGRLEYPDGRFAGA
                     VIAPVLVMSLETMLAAADVGSLGEVSLFGDGLTIIARYPPHPRTVHPGTIPGALRELV
                     EQGEISGTFHADSPVDGLERLYSYRRVSGYSLHITVGRATAEYLGHWRRDMGVVAALA
                     GLLFAVAIGATLMVDRAWRRQRRVARLLELQAHTDALTGLANRRRFFEIADAELARSK
                     RYDTPLSLLMLDIDHFKEVNDTHGHHAGDRVLEELARTCRNILRTVDVAGRVGGEEFA
                     ILLPETALEGAVEVAERLREAIEKTEVTRKEGVPLRITVSIGVAMLTGTANLDTLMSQ
                     ADAALYDAKRNGRNCIRTIALAA"
BASE COUNT          379 a          899 c          920 g          414 t
ORIGIN      
        1 tgccgctcga ccaggatgcc cagcgcgatg agggcctgcg gcccgtggcc gccgcccagc
       61 gcatcggcca ggagatcggc gccctcgccc acgcgcagga ccgagctggc cctctccagg
      121 gccgtgcgga ccgcgggcac cagccgcgtc aagtcgacgg gcttggcgac gaccccgagg
      181 gcgccgaacg tggcggcctt gcggcgcacg tcggcctcgt cccgcaaggg cgcgaggagc
      241 aggaacggca cccgcgtctc gcgggacagc agttgcgcca gctcgagccc ggtcttcccc
      301 gccatggtga tgtcgagcac ggcgagcgcc gggtgcgtgt cgaccgcgac ccgcatggcc
      361 tcttcgccgg tggtggccac gaccacgtcg aagcctgccc gcgacagccc cgcgagcatt
      421 gacgagagca ccacccggtc gtcgtcgacg accagcaggc ggggccttgc cttcgtcatg
      481 ggggcctcat actgtgtcac gattatgcgc gccccggaga ttgccctgaa cgccctgcgc
      541 ctgctgcccg tggtcctcgc cctcggcgtc ctggccgcgc atttctaccg ggcgctggca
      601 tgggtgccct tcggcatcac cgtcgccctg cttccgctgc tgttcattcg tgccccgtgg
      661 gctgcgcggg tgctgcaggc ggccctcgtg gccggcgccc tcgagtggat ccgcaccgcc
      721 gcaggcctcg tggcccttcg ccagtcgatg ggccagccgt atacccgcct cgccatcatc
      781 ctcggcgccg tagccgttgc gacggggctg tgcgcgctca tcttccagtg gcgccccgtt
      841 cgggcgcgat tcgggatggc ggaagcgaaa ggccccggag cctaggccgc cagcgcgatg
      901 gtgcgaatgc agttgcggcc attgcgcttg gcgtcgtaga gggcggcgtc ggcctggctc
      961 atcagggtgt cgaggtttgc ggtcccggtg agcatggcca cgccgatgga taccgtgatg
     1021 cgaagcggca cgccttcctt gcgcgtcacc tcggtcttct cgattgcctc gcgcaggcgc
     1081 tcggccacct caaccgcccc ctcgagcgcg gtttcgggaa gcaggatggc gaactcctcg
     1141 cccccgacgc gccccgccac gtcgacggtg cgaaggatat tcctgcaggt gcgggccagt
     1201 tcctcgagca cccggtcccc ggcatggtgc ccgtgggtgt cgttcacctc cttgaagtgg
     1261 tcgatgtcga gcatcaggag ggacaagggg gtgtcgtagc gcttcgagcg cgccagctcc
     1321 gcatccgcga tctcgaagaa gcggcgccgg ttggccagcc cggtgagggc atcggtgtgg
     1381 gcctgcaatt cgagcaaccg cgcgacgcgc cgctggcggc gccaggcccg gtcgaccatc
     1441 agcgttgccc cgatcgcgac ggcgaacagc agccctgcaa gcgcggccac cacgcccatg
     1501 tcgcgccgcc agtgcccgag gtattccgca gtggcccggc ccacggtgat gtgcagcgaa
     1561 tagcccgaca ccctgcggta ggagtacagg cgctccaggc cgtccacggg ggaatccgcg
     1621 tggaaagtcc ccgaaatctc gccctgctcc acgagttccc ggagcgcgcc gggtatcgtg
     1681 cccgggtgca cggtccgggg gtgcggcgga taacgcgcga tgatggtgag gccgtccccg
     1741 aacaggctga cttcgcccag gctgccgacg tcggcggcgg cgagcatcgt ttcgaggctc
     1801 atgacgagga ccggggcgat gacggcgccg gcgaaacggc cgtcggggta ctcgagacgg
     1861 cccgctatgt cgatcaccca cgggccgccc ggtccgttgt ggtgcggccc ggaaatggcg
     1921 atgctggagg aatgttcgtc gcgaagcatg gcgaagtacg gctggtcttc gacgcccggg
     1981 gcgggagaat ccgcgaactc ggtctggtac accatctggc cggtagcgtc ggtgatgcgg
     2041 atccccacga catcgggcag gttggcgtgg atgtcggcga tgtaggcgcg cccctcctgc
     2101 tcgtcgctgc ccacgcccgc aagccggcgc tcctgctcgg tggccaccgt gtgcagcgcg
     2161 agcttcacgt tgcccaccag gccgctcacg tggctttcga gcacccgggc cacgctgtag
     2221 gtctcgacga cgccctgctc ctcgtgctgc tgccggctgg tgtagatcga ttggccgacc
     2281 aggagggaaa gcagggcgag caataccgcc acgccccgcc aaaggcggct gacgaaactt
     2341 ccgctttgaa tggattccaa ggtccgcgtc ctcccgccat cctccccttc ggccggcggg
     2401 gccaaattgt ccggcatcag caaatcgcgg gattcaggcg caaggcgcac gggaaggcgg
     2461 ggaattgaac ggaccaatgg atactagcgc ctatcgcggg aaattgcctc gtcagtcgag
     2521 gcggtggcgg tggcggtggg ggtcgatcgc attcctgggc acgcgggtcg aggaggcggc
     2581 gtcggaggaa gtcgatcgca atcctgggca cg
//
LOCUS       DASUEV010000226        10363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_395160, whole genome shotgun sequence.
ACCESSION   DASUEV010000226 DASUEV010000000
VERSION     DASUEV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395160"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFV55_07265"
     CDS             complement(<1..242)
                     /locus_tag="VFV55_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit N-terminal domain-containing protein"
                     /protein_id="HEX4944137.1"
                     /translation="MTAIVLDQVDEKKRRFLIVATSAAGAVAAGGVAVPFLGSWFPSA
                     RALAAGAPVEVDVSKIESGQQITVEWRGKPVWVLRRT"
     gene            416..835
                     /gene="mscL"
                     /locus_tag="VFV55_07270"
     CDS             416..835
                     /gene="mscL"
                     /locus_tag="VFV55_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX4944138.1"
                     /translation="MSFASEFKQFALKGNVMDLAVGVIIGAAFGKIVDSLVNDLVMPL
                     VGRVIGNVDFSNLFIALREIPPGIPQTLDAVRKAGVPVLAWGNFATIALNFVILAFII
                     FIMVKQINRLKREAPAAPPPPGEDILLLREIRDNLRK"
     gene            843..2000
                     /locus_tag="VFV55_07275"
     CDS             843..2000
                     /locus_tag="VFV55_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX4944139.1"
                     /translation="MRRLWLVFAQVVTATLAAIFVVSLVRPDWMPWRTNSVVEIRESS
                     VSPAILPASPGRPVSFSDAARKAVNSVVNISATKQVRHRGPLVDDPVLRRFFGERFSV
                     PETQLSLGSGVIVSREGFILTNSHVVEGVTEIQVTLNDGRTVPGRIVGFDTETDLAVV
                     RVSATGLSPITFGQSDQLRVGDVVLAIGDPFSVGQTVTMGIVSAVGREIGSANPFGSF
                     IQTDAAINPGNSGGALVDTNGNLVGINSLIFSKSGGYQGIGFAIPVSLAKKIMEQIIE
                     TGTVTRGWFGVEVANITPELAESLGLSGTRGAIVGGIERGSPADRAGIRLGDVIVGVG
                     DRAVTDLTATLNAIADIPPGKTVQVKLLRRSQEVALAVTVGRRKARPAEEE"
     gene            complement(2006..2755)
                     /gene="tatC"
                     /locus_tag="VFV55_07280"
     CDS             complement(2006..2755)
                     /gene="tatC"
                     /locus_tag="VFV55_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX4944140.1"
                     /translation="MTEDSQETFLSHLFELRDRLVRSIVALGLVFIPTFFYASQLYDL
                     LAMPMIRTLPEGSKMIATGVISPFFIPMKIALMVSFLIALPYILYQAWAFVAPGLYAH
                     ERKLVLPLVVSSTFLFFLGMAFCYFFVFGTVFAFIAQFAPKSITVAPDIEAYFNFVLG
                     MFIAFGVTFEVPVVVVVLVMTGIISVEKLREIRSFVIVGAFVVAAVVTPPDVVSQLLL
                     AIPLCLLYEVGIFVARFVEKRKPEVDAGATQ"
     gene            complement(2752..3213)
                     /gene="tatB"
                     /locus_tag="VFV55_07285"
     CDS             complement(2752..3213)
                     /gene="tatB"
                     /locus_tag="VFV55_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX4944141.1"
                     /translation="MFDIAFSELLVIGVVALVVIGPERLPKVARTMGVLFGRLQRYVS
                     QVKSDINREMELSELSTVKTEFEGAAREFQRDIETKVREAESDIREVEKDIDRQLRSD
                     SAAAPALPGPAHSEPVMAVGTATPDEGPAPASPQLELPIDEPATAPKAGAA"
     gene            complement(3250..3468)
                     /gene="tatA"
                     /locus_tag="VFV55_07290"
     CDS             complement(3250..3468)
                     /gene="tatA"
                     /locus_tag="VFV55_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX4944142.1"
                     /translation="MGSFSIWHWLIVLLIVLLIFGTKKLRNMGADLGGAVRGFKEGMK
                     GEEEKKPPAEIAGTGATLEGEVKDRQKS"
     gene            complement(3490..3846)
                     /locus_tag="VFV55_07295"
     CDS             complement(3490..3846)
                     /locus_tag="VFV55_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX4944143.1"
                     /translation="MKRDDCVFCRIAAGEIPSRKVFEDDEIVAFHDIQPVAPVHLLMV
                     PKAHVDSLADCSPEHAPVLGRMMAMAGRLAREQGATDGFRLIVNTGRVGRQDVYHLHI
                     HIIGGPDRLPRMIATE"
     gene            complement(3846..4175)
                     /locus_tag="VFV55_07300"
     CDS             complement(3846..4175)
                     /locus_tag="VFV55_07300"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX4944144.1"
                     /translation="MAADRTILERLEAAIAARRGADPASSYVAKLNAKGLDAILKKIG
                     EEATETVLAAKSGERGGLIHETADLWFHCLVLLGYQGITVAEVLEELERREGRSGIEE
                     KASRKEP"
     gene            complement(4165..4566)
                     /gene="hisI"
                     /locus_tag="VFV55_07305"
     CDS             complement(4165..4566)
                     /gene="hisI"
                     /locus_tag="VFV55_07305"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX4944145.1"
                     /translation="MVDYLDAIAWNADGLVPAIAQDAASGEVLMLAWMNRESLARTLA
                     TGEAVYWSRSRAKLWRKGETSGHLQRIVEVRLDCDSDALLLRVEQVGGIACHTGRRRC
                     FFNLLEAEGGTARWRTTDPVLADPESFRDGG"
     gene            complement(4576..5337)
                     /gene="hisF"
                     /locus_tag="VFV55_07310"
     CDS             complement(4576..5337)
                     /gene="hisF"
                     /locus_tag="VFV55_07310"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX4944146.1"
                     /translation="MGLAKRIIPCLDVHAGRVVKGVNFVSLRDAGDPVEVARRYDEAG
                     ADEITFLDITASSDARDTIVPVIERVAEQVFIPLTVGGGVRKVEDVRKLLNAGADKVS
                     INTAAVDNPALVAEAARRFGSQAIVVAVDAKAAGPGKWEVYTHGGRKPTGLDAVQWAV
                     KMAGLGAGEILLTSMDRDGTKSGFDLELTRAVSRAVPVPVIASGGVGTLEHLAQGVEI
                     GGADAVLAASIFHYGEFSVGEAKRFMASRGIEMRL"
     gene            complement(5377..6114)
                     /gene="hisA"
                     /locus_tag="VFV55_07315"
     CDS             complement(5377..6114)
                     /gene="hisA"
                     /locus_tag="VFV55_07315"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX4944147.1"
                     /translation="MLIIPAIDLKDGRCVRLEQGDMATATVFSDDPGKTAAHWVALGA
                     RRLHVVDLNGAFAGKPVNESAIKAIIAAVDPDIPVQVGGGIRDLDTIERYLDDGVSYV
                     IIGTAAVKNPGFLHEACDVFPGHIMVGLDAKDGKVATDGWSKLTGHDVVDLARRFQDY
                     GVEAVIHTDIGRDGMLKGLNVEATLRLAQALSVPVIASGGLTNLDDVKRLCAVESDGI
                     VGAVAGRSIYEGRLDFAAAQKLADGEA"
     gene            complement(6140..6775)
                     /gene="hisH"
                     /locus_tag="VFV55_07320"
     CDS             complement(6140..6775)
                     /gene="hisH"
                     /locus_tag="VFV55_07320"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX4944148.1"
                     /translation="MAQRIAIVDYGMGNLRSVAKALEHVAPAATVAITADPAVIRAAD
                     RVVFPGQGAMPDCMKSLAESGLGDAVREAAVAKPFLGLCIGLQMLFERSDEGDTPGLA
                     IFPGEVLAFARAEQTREAGFKIPHMGWNEAWQSRPHALWRGIPDGSRFYFVHSFYCAP
                     RDPTLTVAVSRYPHAFTSAIARDNIFATQFHPEKSSVAGLTLLENFASWTP"
     gene            complement(6814..7401)
                     /gene="hisB"
                     /locus_tag="VFV55_07325"
     CDS             complement(6814..7401)
                     /gene="hisB"
                     /locus_tag="VFV55_07325"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX4944149.1"
                     /translation="MRTAEIQRDTKETQVFVAVNLDGTGKASLASGVPFLDHMLDQIA
                     RHGALDLTVKAKGDLEIDAHHTVEDIGITLGMAVAKALADKKGIRRYGHAYVPLDEAL
                     SRAVVDFSGRPGLEFHVDFTRARVGEFDVDLTREFFQGFVNHALATVHLDNLRGDNSH
                     HQCETIFKAFARALRMAAEPDPRAAGVVPSTKGAL"
     gene            complement(7406..8476)
                     /gene="hisC"
                     /locus_tag="VFV55_07330"
     CDS             complement(7406..8476)
                     /gene="hisC"
                     /locus_tag="VFV55_07330"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX4944150.1"
                     /translation="MKGAAGWVRDEIRALTAYHVAPAAGLVKLDAMENPYRLPPELAD
                     ELGQHLAQVAINRYPDPGAPVLKDALRRSMGIPADLDLILGNGSDEILQVIAVALARP
                     GAVMLALEPSFVMYRMSAITAGMGYAGVDLADDFSLDLRATLAAVERLRPALTWIAYP
                     NNPTGNLFSRDAILAIVAAAPGLVVLDEAYYAFSGGASFLPEVGKHPNLVLVRTVSKL
                     GLAGLRLGLAVGPRDWLAEFDKVRLPYNVNVLSAAAAQFVLARQDVLEAQTRAIVAER
                     ARLEAGLDAIAGVRRFPSAANFVLARVPDAVRAFEGLKSRGILVKNLHGAHPLLANCL
                     RFTVGTPDENARLLAALAPAIG"
     gene            complement(8473..9780)
                     /gene="hisD"
                     /locus_tag="VFV55_07335"
     CDS             complement(8473..9780)
                     /gene="hisD"
                     /locus_tag="VFV55_07335"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX4944151.1"
                     /translation="MAEPRRLSSRDPGFEADLAALLAFEGAQDDSVDRAVAGILAEVQ
                     RRGDEALLEYTRRFDRWSPASAADLEVTRSACREALSRIGHAEREALETAAGRIRRYH
                     EHQHLRGWRIEEEDGTMLGQQVTPLDRVGLYVPGGKAAYPSSVLMNAVAAKVAGVPEL
                     VMVVPTPDGVANDHVLAAASLAGVDRVFRIGGAQAVGALAFGTRSVPAVDKIVGPGNA
                     YVAAAKRRVFGRVGIDMVAGPSEVLVVADASANPDWVAMDLFAQAEHDEVAQSILLTP
                     DAAFADRVLASMRRSIGSMPRRPIIEASLANRGALIVVRDLDEACTIANRIAPEHLEL
                     AVADPDALLPKIRHAGAIFMGHYASESLGDYCAGPNHVLPTSRTARFSSALGVYDFQK
                     RSSLIRVTREAAVRLGATATTLARAEGLEAHARSAELRGEGSK"
     gene            complement(9773..>10363)
                     /gene="hisG"
                     /locus_tag="VFV55_07340"
     CDS             complement(9773..>10363)
                     /gene="hisG"
                     /locus_tag="VFV55_07340"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX4944152.1"
                     /translation="PLLEAAGIVPAEDPEVSRKLILPTGNPGVRVIIVRASDVPTYVQ
                     YGAADLGVAGRDLLIEHGGRGLYQPLDLGIARCRMMVAVPEEFDYPAAVRKGARLRVA
                     TKYIQIAREHFAAKGVHVDLIKLYGSMELAPLVGLADAIVDLVDTGSTLRANRLKAVE
                     EILAVSSRLIVNPASMKVKRAEVLPLIEAFERACRG"
BASE COUNT         1644 a         3556 c         3544 g         1619 t
ORIGIN      
        1 gtgcgccgca ggacccagac gggcttgccg cgccattcga ccgtgatctg ctggccggac
       61 tcgatcttgg aaacgtccac ctccacgggc gcgccggcgg cgagggcacg ggcgctgggg
      121 aaccagctcc ccaggaaggg aacggcgaca cctcccgcgg caactgctcc cgcggcggag
      181 gtggcgacga tcaggaagcg gcgcttcttc tcgtcgactt ggtctaggac gatggcagtc
      241 attgcaggga ggggaggggg gagggagggg tagccgggtg ggaagagtgt cgcccgccat
      301 tttagccgat tcccggcaag gacttacagc cccatgggcg ccggaaaggg ttgacgcacc
      361 ccctaccccc ccgtagattc agcagttcgg ccgccatcga ccaccggggg aatccatgag
      421 ttttgcttct gaattcaagc agttcgcact gaagggcaat gtcatggacc tggccgtggg
      481 cgtgatcatc ggggcggcct tcggaaagat cgtcgactcg ctcgtcaacg acctcgtgat
      541 gccgttggtg ggccgcgtga tcggcaatgt ggacttctcg aacctcttca tcgccctccg
      601 ggaaatcccg ccgggcatcc cgcagaccct cgatgcggtc aggaaggcgg gcgtcccggt
      661 cctcgcgtgg gggaacttcg ccaccatcgc gctcaatttc gtgatcctcg cgttcatcat
      721 cttcatcatg gtgaagcaga tcaaccggct gaagcgcgag gcgcccgcgg cgcccccccc
      781 gccgggcgag gacatcctgc ttctgcgcga aatccgcgac aacctgagga aataggtcct
      841 tggtgcggcg gctctggctg gtcttcgcgc aggtcgtcac ggccacgctg gcggcgatct
      901 tcgtcgtgag cctggtcagg ccggactgga tgccgtggcg aacgaacagc gtggtcgaga
      961 tccgcgagtc ctccgtttcc cccgcgattc tcccggcctc cccgggccgg ccggtttcgt
     1021 tcagcgacgc cgcccgcaag gccgtcaact cggtcgtcaa catctcggcg acgaagcagg
     1081 tccggcaccg cggccccctg gtcgatgacc cggtcctgcg ccgcttcttc ggcgagcgct
     1141 tcagcgtccc cgagacgcag ctctccctcg gctcgggcgt catcgtgagc cgggaggggt
     1201 tcatcctcac caactcgcat gtcgtcgagg gcgtgaccga gatccaggtc accctcaacg
     1261 acgggcgcac ggtcccgggc cggatcgtcg gtttcgacac cgaaacggac ctcgccgtcg
     1321 tgcgcgtctc ggccacgggt ctctcgccca tcaccttcgg ccagtccgac cagctccgcg
     1381 tgggcgatgt cgtgctcgcg atcggcgatc cgttcagcgt cggccagacc gtgaccatgg
     1441 gcatcgtgag cgccgtcggg cgcgagatcg ggtcggcgaa tcccttcggg agcttcatcc
     1501 agaccgatgc cgcgatcaac ccgggcaact cgggcggcgc cctggtggac acgaacggca
     1561 acctcgtggg catcaactcc ctcatcttct cgaagtcggg gggctaccag gggatcggct
     1621 tcgcgattcc cgtgagcctc gccaagaaga tcatggaaca gatcatcgag accggcaccg
     1681 tcacccgcgg gtggttcggc gtggaagtcg ccaacatcac gccggaactc gccgagtcgc
     1741 tgggcctgtc cggcacgcgc ggcgccatcg tcggcggcat cgagcgcggc agccccgccg
     1801 atcgcgcggg catccgcctg ggggacgtga tcgtcggcgt cggcgatcgc gccgtcaccg
     1861 acctcaccgc gacgctcaat gcgatcgccg acatcccgcc cggcaagacc gtgcaggtga
     1921 agctgctgag aaggagccag gaagtggccc tggcagtcac cgtagggcgg cgaaaggccc
     1981 gcccggcaga ggaggagtag gcgcgctact gcgtggcgcc ggcgtcgacc tcgggcttgc
     2041 gtttctcgac gaaccgcgcg acgaagatcc ccacctcgta gagcaggcac agcgggatcg
     2101 cgagcaggag ctgcgagacc acgtcggggg gcgtcacgac tgccgccacc acgaacgcgc
     2161 cgacgatcac gaaggaccgg atctcgcgca gcttctccac ggagatgatt ccggtcatca
     2221 cgaggaccac gaccacgacg ggcacctcga aggtcacccc gaaggcgatg aacatcccga
     2281 ggacgaaatt gaagtaggcc tcgatgtcgg gggccacggt gatcgacttg ggcgcgaact
     2341 gggcgatgaa cgcgaagacg gtcccgaaga cgaaaaagta gcagaacgcc atgccgagga
     2401 agaacaggaa cgtgctcgac acgacgagcg gcagcacgag cttcctctcg tgggcataaa
     2461 gccccggcgc cacgaaggcc cacgcctggt agaggatgta gggcagcgcg atgaggaacg
     2521 acaccatcag cgcgatcttc atcgggatga agaaaggcga gatgaccccg gtcgcgatca
     2581 tcttcgagcc ctccgggagc gtgcgtatca tcggcatcgc gagcaggtcg tagagctggg
     2641 aggcgtagaa gaacgtggga atgaagacca ggcccagcgc gacgatcgag cgcacgagcc
     2701 ggtcgcgcag ctcgaacagg tgcgagagga aggtctcctg ggagtcctcg gtcatgcggc
     2761 gccggccttc ggggccgtcg ccggctcgtc gatcgggagc tccagttgcg gcgaggcggg
     2821 cgccggacct tcatccggtg tcgcggtccc cactgccatc acgggttcgc tgtgcgcagg
     2881 cccgggcagc gcgggggccg cggcactgtc gctgcgcaat tgccggtcga tgtccttctc
     2941 gacctcgcgg atgtcgcttt ccgcctcgcg caccttcgtt tcgatgtcgc gctggaactc
     3001 gcgcgctgcg ccctcgaact cggtcttcac cgtggacagc tcggacagct ccatctcccg
     3061 gttgatgtcc gatttcacct gcgagacgta gcgctgcagg cggccgaaga ggacccccat
     3121 cgtgcgggcg accttcggca ggcgctcggg gccgatgacg acaagcgcca ccaccccgat
     3181 caccagcagc tcggagaacg cgatatcgaa catggattcg acgggcggag cgttgccgcc
     3241 ccccggcggt cagctctttt gcctgtcctt gacctcgccc tcgagcgtcg cgccggtgcc
     3301 ggcgatctcc gcggggggct tcttctcttc ctcgcccttc atgccttcct tgaagccgcg
     3361 aacggcgccg cccaggtcgg cacccatgtt gcgcagtttc ttcgtgccga agatgagcag
     3421 cacgatcagg aggacgatca accagtgcca gatgctgaat gaacccatgg aacgctcctt
     3481 tcggcgccgc tattccgtcg cgatcatgcg cggaagtcga tcggggccgc cgatgatgtg
     3541 gatatgcaaa tgatacacgt cctgtcgccc caccctgccc gtgttcacga tcaggcggaa
     3601 gccgtccgtg gccccctgtt cgcgcgccag gcgcccggcc atggccatca tccggcccag
     3661 cacgggggca tgctcggggc tgcaatcggc cagggaatcg acatgcgctt tcgggaccat
     3721 gagcagatgg accggcgcga ccggctggat gtcgtggaag gcgacgatct cgtcgtcctc
     3781 gaagaccttc ctcgacggga tctcccccgc cgcgatcctg cagaagacgc agtcgtccct
     3841 cttcatcacg gttcctttct cgacgccttt tcctcgatcc ccgagcggcc ctcgcgccgt
     3901 tcgagctcct cgagcacttc cgccacggtg attccctggt acccgagcag gacgaggcag
     3961 tggaaccaga ggtcggccgt ctcgtggatc aggccgccac gctccccgga cttggcggcg
     4021 aggacggttt ccgtggcctc ctcgccgatc ttcttgagga tcgcgtccag ccccttcgcg
     4081 ttgagcttcg ccacgtagga ggaggcgggg tccgcccccc tgcgcgccgc gatggccgcc
     4141 tcgagccgct cgaggatcgt cctgtcagcc gccatcgcgg aagctttccg ggtccgcgag
     4201 gacggggtcg gtggtgcgcc agcgcgccgt gccgccttcc gcctcgagca ggttgaagaa
     4261 gcagcgccgc cggcctgtgt ggcaggcgat cccgcccacc tgctccacgc gcaggagcag
     4321 cgcgtccgag tcgcagtcga gccggacctc gacgatgcgt tgcaggtgcc ccgaggtctc
     4381 gcccttgcgc cagagcttcg ctcgcgagcg cgaccagtac accgcctcgc ccgtggcgag
     4441 cgtccttgcc agggactcgc ggttcatcca ggcgagcatg agcacctcgc ccgaggccgc
     4501 atcctgcgca atggcgggca cgagcccgtc ggcgttccac gcaatggcgt cgaggtaatc
     4561 cacgatgggc gcccgctaga gcctcatctc gatgccgcgg ctggccatga agcgcttggc
     4621 ctcccccacc gagaactcgc cgtagtggaa tatcgacgcg gcgagcaccg cgtcggcgcc
     4681 gccgatctcc acgccctgcg ccaggtgctc gagggtgccg acgccgccgc tcgcgatcac
     4741 cggcaccggg accgcgcgcg acaccgcacg ggtgagctcg aggtcgaacc ccgacttcgt
     4801 tccgtcgcgg tccatgctgg tgaggaggat ctcgcccgcc ccgagccccg ccatcttcac
     4861 ggcccattgc acggcgtcca ggccggtggg cttgcgcccg ccgtgcgtgt acacctccca
     4921 cttgcccggg ccggcggcct tggcgtccac ggcgacgacg atggcctgcg agccgaagcg
     4981 ccgcgcggcc tccgcgacca gcgcggggtt gtccacggcg gcggtgttga tgctcacctt
     5041 gtccgcaccc gcattgagga gttttcgcac gtcctcgacc ttgcgcacgc ccccgcccac
     5101 cgtgagcggg atgaacacct gctcggcgac gcgttcgatc accggcacga tggtgtcgcg
     5161 ggcgtcgctc gaggccgtga tgtcgaggaa ggtgatctcg tccgctcccg cctcgtcgta
     5221 gcggcgcgcg acttccaccg ggtcgcccgc gtcgcgcagg ctcacgaagt tcacgccctt
     5281 caccacgcgg cccgcatgga cgtccaggca gggaatgatg cgcttggcga ggcccatggt
     5341 cgcgcggttg cggaagcgtg gccggcgtcg cgccggtcag gcctcaccgt cggccagctt
     5401 ctgcgccgcg gcgaaatcga ggcgtccttc gtagatggac cgcccggcga ccgcgccgac
     5461 gatgccatcg ctttccaccg cgcacaggcg cttcacgtcg tcgaggttgg tgagcccgcc
     5521 gctcgcgatc accgggacgg aaagcgcctg ggcaagccgc aacgtggcct ccacgttgag
     5581 ccctttcagc atgccgtcgc gcccgatgtc ggtatggatg acggcctcga cgccgtaatc
     5641 ctggaagcgc ctggcgagat ccacgacgtc gtggccggtc agcttggacc acccgtccgt
     5701 ggccaccttg ccgtccttcg cgtcgagccc gaccatgatg tggccgggaa acacgtcgca
     5761 agcctcgtgc aggaacccgg ggttcttcac cgccgcagtc ccgatgatca cgtaggagac
     5821 gccgtcgtcc aggtagcgtt cgatcgtgtc gaggtcgcgg atgccgccgc ccacctgcac
     5881 cgggatgtcg ggatccaccg cggcgatgat cgccttgatc gccgattcgt tgacgggctt
     5941 gccggcgaag gcgccattca ggtccaccac gtgcagccgg cgcgcaccca gcgccaccca
     6001 gtgggcggcc gtcttgcccg ggtcgtcgga gaacacggtg gcggtagcca tgtcgccctg
     6061 ctcaaggcga acgcagcggc catccttgag gtcgatcgcg ggaatgatca gcatgggagc
     6121 ggtcggcgga gccggttcct cacggggtcc atgaagcgaa gttttcgaga agcgtgagcc
     6181 ccgcgacgct gcttttctca ggatggaatt gcgtggcgaa gatgttatcc cgcgcgatgg
     6241 ccgaggtaaa ggcatgcgga tagcgcgaca ccgcgacggt gagcgtcggg tcgcgcggcg
     6301 cgcaataaaa gctgtgcacg aaatagaaac gggacccgtc cgggatccct cgccacaggg
     6361 cgtggggccg cgactgccac gcctcgttcc agcccatgtg cgggatcttg aagccggcct
     6421 cccgcgtctg ctcggcgcgg gcgaacgcga ggacctcgcc cgggaagatg gcgaggcccg
     6481 gcgtgtcgcc ctcgtcgctg cgctcgaaga gcatctgcag accgatgcac agcccgagga
     6541 acggtttcgc gacggcggct tcccgcaccg cgtcgcccag gccgctttcc gcgagggact
     6601 tcatgcagtc cggcatggct ccctgccccg ggaagacgac ccggtccgcc gcgcggatca
     6661 ccgccgggtc ggcggtgatc gcgaccgtgg ccgccggcgc cacgtgctcg agtgccttgg
     6721 ccaccgagcg aaggttgccc atcccgtaat cgacgattgc gatgcgctgt gccattgggg
     6781 cgccggggca atgcaccggc ctgaaagggt tgcctagagg gctcccttgg tcgaggggac
     6841 gacgcccgca gctcgcggat cgggttccgc cgccatgcgc agcgcgcgcg cgaacgcctt
     6901 gaagatcgtt tcgcactggt ggtgcgagtt gtcgccgcgc aggttgtcga ggtgcacggt
     6961 ggcgagcgcg tgattcacga agccctggaa gaactcgcgc gtgaggtcga cgtcgaactc
     7021 gcccacccgg gcccgcgtga agtccacgtg gaactcgagg ccgggacgtc ccgagaaatc
     7081 gaccaccgcg cgcgagagcg cctcgtcgag cgggacatag gcatgcccgt agcggcggat
     7141 gcccttcttg tcggcaagtg ccttcgcgac cgccatgccg agcgtgatcc cgatgtcctc
     7201 gaccgtgtgg tgagcatcga tctcgaggtc gcccttcgcc ttcacggtga ggtccagcgc
     7261 accgtggcgg gcaatctggt cgagcatgtg gtcgaggaac ggcacgcccg aggcgaggct
     7321 cgccttgccc gtgccgtcga ggttcacggc cacgaagacc tgggtttcct tggtgtctcg
     7381 ctggatttcc gcggttcgca tgggttcaac cgatcgccgg ggccagtgcc gcgagaagtc
     7441 tcgcgttttc gtcaggggtg ccgacggtga atcgcaggca gttcgcgagg agcggatggg
     7501 cgccgtgaag gttcttgacc aatataccac gcgacttgag gccctcgaac gcccgcacgg
     7561 cgtccggtac gcgtgccagg acgaagttcg cggccgaggg gaagcgccgc acgcccgcga
     7621 tcgcatccag cccggcttcc aggcgggcgc gttccgccac gatcgcgcgc gtctgcgcct
     7681 cgagcacatc ctggcgggca agcacgaatt gcgctgccgc ggctgaaaga acgttcacgt
     7741 tgtagggcag gcgaaccttg tcgaattccg ccagccagtc gcgcggcccg acggcaagac
     7801 ccagccgcag cccggccagg cccagcttcg acacggtgcg aacgagcacg aggttggggt
     7861 gctttccgac ctcgggcagg aagctcgcgc cgcccgagaa tgcgtagtac gcctcgtcga
     7921 gaacgacgag ccccggcgct gccgcgacga tggcgaggat cgcgtcgcgg gaaaacaggt
     7981 ttccggtagg gttattgggg taggcgatcc aggtgagcgc gggacgcagc cgttcgaccg
     8041 cggccagggt ggcccgcagg tccagcgaga aatcatcggc cagatcaacg ccggcatagc
     8101 ccatgccggc ggtgatggcg ctcatccggt acatcacgaa cgagggttcg agcgccagca
     8161 tcacggcgcc gggcctcgcc agcgccacgg cgatcacctg caggatctcg tcggaaccgt
     8221 tgccgaggat caggtcgagg tccgcgggaa tgcccattga ccggcgcagg gcgtccttca
     8281 gaaccggggc gccgggatcg ggatagcggt tgatcgcaac ttgggccagg tgctggccga
     8341 gttcgtccgc gagttccggc ggaaggcggt acggattttc catcgcgtcg agcttcacca
     8401 gcccggcagc gggcgcgaca tggtaggccg tcagcgcccg gatctcgtcg cgcacccagc
     8461 ccgcggcgcc tttcacttcg aaccctcgcc gcgcagctcc gcgctgcgcg cgtgcgcctc
     8521 caggccctcg gcgcgagcca gcgtcgtcgc ggtcgccccg agccgcacgg cagcctcgcg
     8581 cgtgacgcga atcaggctcg agcgcttctg gaagtcgtag accccgagcg ccgaggaaaa
     8641 acgcgcggtg cgcgaggtgg gcagcacgtg gttcggcccc gcgcagtagt cgcccaggga
     8701 ctccgaggcg taatggccca tgaagatggc acccgcgtgc cggatcttcg gcagcagcgc
     8761 gtccggatcg gccaccgcga gctccaggtg ttccggcgcg atccggttgg cgatcgtgca
     8821 cgcctcgtcc aggtcgcgca cgacgatgag cgcgccgcga ttggcgagcg atgcctcgat
     8881 gatgggccgt cgcggcattg acccgatgct ccttcgcatc gacgccagca cgcgatcggc
     8941 gaacgccgcg tcgggggtga gcaggatcga ctgcgcgact tcgtcgtgct cggcctgcgc
     9001 gaagagatcc atcgccaccc agtcggggtt cgcggaggcg tcggccacga cgaggacttc
     9061 cgaggggccg gccaccatgt cgatgccgac gcgcccgaag acgcggcgct tggcggcggc
     9121 cacgtacgca ttgcccggcc cgacgatctt gtcgacggcc ggcacgctgc gtgtcccgaa
     9181 agcgagcgcg ccgaccgcct gggcgccccc gatgcggaag acccggtcca cgcccgcgag
     9241 cgaggcggcc gccagcacgt ggtcgttggc cacgccgtcg ggtgtgggca cgaccattac
     9301 cagctcgggc accccggcga ccttcgcggc caccgcgttc atcaggaccg aggacgggta
     9361 cgcggccttg ccgccgggca cgtagaggcc gacacggtcc agcggcgtca cctgctggcc
     9421 gagcatcgtg ccgtcctcct cctcgatgcg ccagccgcgc aggtgctggt gctcgtggta
     9481 gcggcggatg cgcccggcgg cggtttccag cgcctcgcgc tcggcgtgcc cgatgcgcga
     9541 cagcgcttcg cggcaggcgg accgcgtcac ctcgagatcg gcggcggaag cgggcgacca
     9601 gcgatcgaag cgccgggtgt attccagcag cgcctcgtcc ccgcgccgct gcacttccgc
     9661 gaggatcccg gcgaccgcac ggtcgacgct atcgtcctgc gcgccctcga aggcgaggag
     9721 cgcggcgaga tcggcctcga agccgggatc gcggctcgac aggcggcgcg gctcagccac
     9781 ggcatgccct ctcgaaggcc tcgatgagcg gcagcacctc ggcgcgcttc accttcatcg
     9841 aggcgggatt gacgatgagc cggctcgaca ccgccaggat ctcctcgacg gccttcaggc
     9901 ggttggcgcg cagcgtgctg ccggtgtcga cgaggtccac gatggcgtcc gcgagcccga
     9961 cgaggggggc aagctccatg gacccgtaga gcttgatgag gtccacgtgc acgcccttgg
    10021 cggcgaagtg ctcgcgcgcg atctggatgt acttcgtggc cacgcgcagc cgggcgccct
    10081 tgcgcaccgc cgcgggatag tcgaactcct ccggcacggc caccatcatc ctgcagcgcg
    10141 cgatgccgag gtcgaggggc tggtagaggc cgcggccgcc gtgctcgata agcaggtcgc
    10201 gcccggccac gccgaggtcg gcggccccgt actgcacgta ggtcgggacg tccgaggcac
    10261 gcacgatgat cacgcggaca ccggggttgc cggtaggcag gatgagcttg cgcgagacct
    10321 cggggtcctc cgcgggaacg atgcccgccg cctcgagcag cgg
//
LOCUS       DASUEV010000227         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_74112, whole genome shotgun sequence.
ACCESSION   DASUEV010000227 DASUEV010000000
VERSION     DASUEV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74112"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFV55_07345"
     CDS             complement(<1..407)
                     /locus_tag="VFV55_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX4944153.1"
                     /translation="MDLSKATFWGYKRENGRIGVRNHVIILPVDDLSNAAALAVENNI
                     KGTMALPHPYGRLQFGADLDLHFRTLIGTGSNPNVAAVVVICIEEQWAKKVADGIAAT
                     GKPVAWFGIEQHGDHDTIMRASKKAKEFVQWAS"
     gene            complement(660..941)
                     /locus_tag="VFV55_07350"
     CDS             complement(660..941)
                     /locus_tag="VFV55_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HEX4944154.1"
                     /translation="MIHFVVHDEGDSVGVVVVEGVKAGDTLTGWIMDQDRDIQVKAAS
                     DIPIGHKLAIKALARDATVIKYGTDIGRVVAPIGVGEHAHVHNIKTKRW"
     gene            1029..1796
                     /locus_tag="VFV55_07355"
     CDS             1029..1796
                     /locus_tag="VFV55_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX4944155.1"
                     /translation="MPATARRAAAPALAPGRPLYEELRAQLTEGIATGRWKPGEAIPP
                     EAMLAGHFGVAIGTVRKAVDSLVAQGALVRCQGKGTYVTSHDGRRLMFHFFHIVGADG
                     SKAYPEVRTVSFARGRADADEAQALAIAPDERVIRIRNLLALGGHPVIVDDIALPAAL
                     FAGLTERIFTARDNTIYHLYQSRYGINVLRTDERLRARLAAGATASLLGVAAGSPLLE
                     IRRVALTFRDRPVELRVSRVNTAAHVYHNTFGKGEAL"
     gene            1800..2693
                     /locus_tag="VFV55_07360"
     CDS             1800..2693
                     /locus_tag="VFV55_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX4944156.1"
                     /translation="MQTALLILPNFVLILVGLALARGFDYGRGFWEGLEKLVYFVLFP
                     ALLLRSLAVSTLDLASLGKVVACAWAVMLLGMALGWLARPLFRLEARVAASCFQCAFR
                     FNSYIGFAVAGSVFGSPGVATAALVFGTVIPLANLGAVGMLAAHARTNLWLELARNPL
                     VMSTLAGFAWNLAGVPMPGFLDQTLDLLGQSALPAGLLAVGAALRIERGQAGTAAHAW
                     WLGVKLGALPLAAWALVKMLGLSGVEAGVLVLWAALPTASSAYILAVRMGGDGPSVAT
                     QITAGTVIAMATIPLWVAALP"
     gene            complement(2793..3200)
                     /gene="rplS"
                     /locus_tag="VFV55_07365"
     CDS             complement(2793..3200)
                     /gene="rplS"
                     /locus_tag="VFV55_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX4944157.1"
                     /translation="MDLIRKLEQEEIARLGRTIPDFSPGDTVIVKVQVVEGDKKREQA
                     YEGVVIAKRNKGLNSSFIVRKISSGEGVERTFQTYSPTVASIEIKRRGAVRRAKLYYL
                     RDRSGKSARIREKLKSSTDREVLPPVEAEPDKA"
     gene            complement(3296..4060)
                     /gene="trmD"
                     /locus_tag="VFV55_07370"
     CDS             complement(3296..4060)
                     /gene="trmD"
                     /locus_tag="VFV55_07370"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX4944158.1"
                     /translation="MHFDVVTIFPEMFEAVTRHGITRRALEEGGFDFRAWNPRDFVND
                     PHRTVDDRPYGGGPGMVMLPGPLEQCIDAAVARQSAAGVERPQVVLLSPRGERFTDAM
                     ARRLAMSPGVVLVAGRYEGVDERLIARRVDREISIGDYVTSGGELPAMVMMDAIVRLL
                     PGSLNDSGSASQDSFSEGLLDWPHYTRPEEWKGERVPEVLLSGNHAAIARWRRKQALA
                     RTWARRPDLLENRALSKQDAELLEEYRREQQAQERQ"
     gene            complement(4063..>4533)
                     /gene="rimM"
                     /locus_tag="VFV55_07375"
     CDS             complement(4063..>4533)
                     /gene="rimM"
                     /locus_tag="VFV55_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205462.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX4944159.1"
                     /translation="GPFGIGGWIKLRTFTESPDGLSGHSSWWLRTAAGWRSAVLEDFK
                     VRPAAVSAKLQGVDDRNAAELLRGFDVAVTRDDLGEAKEGEFYWVDLVGLAVVNLQDE
                     VLGQVEELLRTGGSDVLVVKGDRERLIPFIPDFVKSVDRDAKRITVDWEAGYDV"
BASE COUNT          637 a         1630 c         1449 g          817 t
ORIGIN      
        1 tcgctggccc actgcacgaa ctccttcgcc ttcttcgagg cgcgcatgat cgtgtcgtgg
       61 tcgccgtgct gctcgatgcc gaaccaggcg acgggcttgc cggtggcggc gatgccgtcg
      121 gccaccttct tcgcccactg ctcctcgatg cagatgacga ccacggcggc gacgttggga
      181 ttggaacccg tgccgatgag cgtgcggaag tgcaggtcca ggtcggcgcc gaactgcagc
      241 cgcccgtacg ggtgcggcag cgccatcgtg cccttgatgt tgttttccac cgccagcgcc
      301 gcggcattgg acaggtcgtc gacgggcagg atgatgacgt ggttgcgcac gccgatgcgg
      361 ccgttttccc gcttgtatcc ccagaacgtt gccttgctca gatccatgtt ggattcccct
      421 agtgcctgta gttaccgcaa acctttagtg ccggtattta ccgcgaaccc tggaacacgg
      481 atgaacacgg ataaaggcgg atgaacacgg atatttgatt gtcaggtagc ggcaatcggg
      541 ggcgtcgtgc cgcgtcatcg cgcaccacgc caagcgcagc cgcgaacccg acgaattcat
      601 ccgtgttcat ccgcctttat ccgtgttcat ccgtgttcca gggtttgcgc ccgcatcact
      661 caccagcgct tcgtcttgat gttgtggaca tgggcatgct cgcccacgcc gatgggggcc
      721 accacgcgcc cgatgtcggt gccgtacttg atcacggtcg cgtcccttgc cagcgccttg
      781 atggcgagct tgtggccgat gggaatgtcg ctggcggcct tgacctgtat gtccctgtcc
      841 tggtccatga tccagccggt gagggtgtcg ccggccttca cgccctcgac caccaccacg
      901 ccgacggaat cgccttcgtc atgcactacg aaatggatca tcgctcgtcg ctccacatgc
      961 ggaacggcga tgctacttcc cgggcgcggg gcgagtcaac tagatgagtt gcatgttatg
     1021 cttccgccat gcccgccacc gcccgccgtg ccgccgcccc cgcgctcgcg cccggccgcc
     1081 cgctctacga ggagttgcgc gcccagctga ccgaaggcat cgccaccggc cgctggaagc
     1141 cgggcgaggc gatcccgccg gaggccatgc tggcaggcca tttcggcgtg gccatcggca
     1201 cggtccgcaa ggcggtggac agcctcgttg cgcagggggc gctggtgcgc tgccagggca
     1261 agggcacgta cgtcacgtcg cacgacggcc ggcggctcat gttccacttc ttccacatcg
     1321 tcggcgcgga cggctcgaaa gcctaccccg aggttcgcac ggtgtcgttc gcacgcgggc
     1381 gcgccgacgc ggacgaggcg caggccctcg cgatcgcgcc cgacgagcgc gtgatccgca
     1441 tccgcaacct cctcgccctc gggggccatc cggtcatcgt ggacgacatc gcgctgccgg
     1501 cggcgctgtt tgccggcctc acggagcgca tcttcaccgc gcgcgacaac acgatctatc
     1561 acctctacca gagccgctac gggatcaacg tgctgcgcac cgacgagcgg ctgcgcgcga
     1621 ggctcgccgc gggcgcgacg gcctcgctgc tcggcgtggc cgccggcagc ccgctcctcg
     1681 agatacgccg cgtcgcgctc accttccgcg accgtcccgt ggagcttcgc gtctcgcgcg
     1741 tgaacaccgc cgcgcacgtc tatcacaaca ccttcggcaa gggcgaggcg ctatagccga
     1801 tgcagaccgc gctcctcatc ctccccaatt tcgtgctcat cctcgtgggg ctggccctgg
     1861 cgcgcgggtt cgactacggg cgcgggttct gggaggggct cgagaagctc gtctacttcg
     1921 tgctcttccc ggcgcttctg ttgcgctcgc tcgccgtctc caccctcgac ctggccagcc
     1981 tgggcaaggt ggtggcctgc gcgtgggccg tcatgctgct cgggatggct ctgggatggc
     2041 tcgcgcggcc gctgttccgg ctggaggcgc gcgtggcggc ctcgtgcttc cagtgcgcct
     2101 tccgcttcaa ctcctacatc ggcttcgcgg tggccggcag cgtgttcggc tccccgggcg
     2161 tggcgacggc ggcgctcgtc ttcggcacgg tgatcccgct ggcgaacctc ggcgccgtgg
     2221 gcatgctggc cgcgcatgcg cgcacgaacc tgtggctcga gctggcgcga aacccgctgg
     2281 tcatgtcgac gctcgccggc ttcgcgtgga atctcgcggg cgtgccgatg cccggctttc
     2341 tcgaccagac gctcgatctg ctcgggcagt cggccctgcc ggcggggctg ctggccgtcg
     2401 gtgcggcact gcgcatcgag cgcggccagg cgggcacggc ggcgcatgca tggtggctgg
     2461 gcgtgaagct cggcgcgctt ccgctcgcgg cctgggccct ggtgaagatg ctgggacttt
     2521 ccggcgtcga ggccggcgtg ctcgtgctgt gggcggcact gcccaccgcc tccagcgcct
     2581 acatcctcgc ggttcgcatg ggtggtgacg ggccgtcggt ggccacgcag atcaccgccg
     2641 gaacggtcat tgcgatggcc acgatcccgc tgtgggtcgc cgccctgccg tgaggcgcag
     2701 tgcaacgcgc aatcccaccg cgcggtcgga gccgggatgc agaaagggcc gcgctgggcg
     2761 gcccttcttc gttgcgggga gcgaccaggg cttcaggcct tgtccggctc cgcctcgacc
     2821 ggcggcaaca cctcgcggtc ggtcgagctc ttgagcttct cgcggatgcg cgccgacttg
     2881 cccgagcgat cgcgcaggta gtagagcttg gcccggcgca cggcgccgcg gcgcttgatc
     2941 tcgatggagg cgaccgtcgg cgagtacgtc tggaacgtgc gctccacgcc ctcgccgctg
     3001 gagatcttgc gcacgatgaa ggaggaattg agccccttgt tgcgcttggc gatcaccacg
     3061 ccttcgtagg cctgctcgcg cttcttgtcg ccttcgacca cctgcacctt gacgatcacc
     3121 gtgtcgccag gggagaagtc ggggatcgtg cgcccgaggc gcgcgatttc ttcctgctcg
     3181 agtttgcgga tgagatccat gaacggttcc tttcgctgtc cggccttccg gccttccgcg
     3241 cagagcagcc cgcgggctgg tgcacggggt ttgtttttcg cttgacccgt tgcctctact
     3301 gccgttcctg cgcctgctgc tcgcggcggt attcctcgag caactccgca tcctgcttcg
     3361 acagcgcccg gttctccagg agatccggac gccttgccca cgtgcgcgcc agtgcctgct
     3421 tgcggcgcca ccgcgcgatc gcggcatggt tgcccgacaa cagcacctcg ggcacccgtt
     3481 cacctttcca ttcctccggc cgcgtgtagt gcggccagtc cagcagtcct tccgagaacg
     3541 aatcctgcga cgcgctcccg gaatcgttga ggctgcccgg caggagcctc acgatcgcat
     3601 ccatcatcac catcgccggc aactcgccgc cggaggtcac atagtcgccg atcgagatct
     3661 cccggtccac ccggcgcgcg atgaggcgtt catccacccc ctcgtagcgc ccggcgacga
     3721 gcaccacgcc cggcgacatc gccaaccgcc ttgccatcgc gtcggtgaag cgctcgcccc
     3781 gcggcgacag cagcaccacc tgcggccttt ccaccccggc ggccgactgc cttgccaccg
     3841 cggcatcgat gcactgttcc agcggccccg ggagcatcac catccccggt ccgcccccgt
     3901 agggacggtc gtcgaccgtg cggtgcggat cgttcacgaa atcgcgcggg ttccaggcgc
     3961 ggaagtcgaa accgccttct tccagcgcac gccgcgtgat gccgtggcgg gtcaccgcct
     4021 cgaacatctc cgggaagatc gtgaccacgt cgaaatgcat catcagacgt cgtaccccgc
     4081 ttcccagtcc accgtgatgc gtttcgcgtc ccggtcgacc gacttcacga aatccggaat
     4141 gaaggggatc aaccgctccc gatcgccctt cacgaccagc acgtccgacc cgccggtcct
     4201 cagcaattcc tcgacctgcc cgagcacctc atcctgcagg ttcaccaccg cgagccccac
     4261 caggtccacc cagtagaact cgccttcctt cgcctccccg agatcgtccc gggttaccgc
     4321 gacgtcgaag ccgcggagca gctcggcggc attgcggtcg tcgacgccct gcagcttcgc
     4381 actcaccgcg gccgggcgga ccttgaagtc ctcgagcacc gcggagcgcc atcccgccgc
     4441 ggtccgcagc caccacgaag agtgacccga gagcccgtcc ggagattcgg tgaaggtcct
     4501 gagcttgatc cagcctccga tgccgaaggg gcc
//
LOCUS       DASUEV010000228         7029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1564142, whole genome shotgun sequence.
ACCESSION   DASUEV010000228 DASUEV010000000
VERSION     DASUEV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564142"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1482)
                     /gene="tolB"
                     /locus_tag="VFV55_07380"
     CDS             complement(211..1482)
                     /gene="tolB"
                     /locus_tag="VFV55_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX4944160.1"
                     /translation="MKRIILAILVACVALAARAQLTIDITTSGGRQIPIAILPFAGET
                     AQPQVVSTVVGSDLARSGLFRLVNAVGVSPLPTEPSEVNFVDWQARSSEALVIGKVEA
                     QADGRVEVRFRLFDVQKQSQLASYSYVVSPAQLRATAHRIADVIYEKLTGEKGVFSTK
                     ITYVVKRGTRFELQVSDADGANAQAVLASNEPIISPAWSPDGSRIAYVSFDQKKPVVV
                     MQNLAQGTTRILANYRGNNSAPAWSPDGSKLAVTLTKDDIAQIYVIPAGGGEPRRLTV
                     TPAIDTEPSFSPDGKWIAFTSDRGGSPQIYRMPADGGPAQRLTFEGTYNARPRYSPDG
                     KSIAFVQREAGRFRIAVLEIGTGQVTVLTDGTLDDSPSYAPNGTMILYETQTGGRGQL
                     AAVSSDGRVRQRLVSSSGDVRDPAWGPLPTN"
     gene            complement(1514..2407)
                     /gene="tolA"
                     /locus_tag="VFV55_07385"
     CDS             complement(1514..2407)
                     /gene="tolA"
                     /locus_tag="VFV55_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX4944161.1"
                     /translation="MARPESQEKPRQPGKIRSIILAVAVHAAFFALIVFGVTWQSRPT
                     PPLQAELWDKLPTGKPGAAPKAEPQPPKVEPPQAEPPKPEPPRAEPKPEPPKPEPPKA
                     EPRPEPPKPDPAIAEKKEREKKERAKKEQEKRELEKREHDRKVQETKAREAKAAAEAK
                     AVADAKAKAVADAKAKAVAEARAKAEAEAQAKSAAEAATQARLSEIDRYRGRIRDKIR
                     GKANVPDTVRGKPEVQVRITILPGGEVLDARIVRPSTNPAYDDAILRAIRSASPLPVP
                     PASSELFPTFRDLILNFEHER"
     gene            complement(2418..2825)
                     /gene="tolR"
                     /locus_tag="VFV55_07390"
     CDS             complement(2418..2825)
                     /gene="tolR"
                     /locus_tag="VFV55_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX4944162.1"
                     /translation="MRRRRAMSEINVVPYIDVMLVLLIIFMVAAPLINPGQIDLPQVG
                     AKLDPAIAPLEVRIRENGELSIVDRSRSPDELLVKRPQLLEIVRRAQRENAEQAVVIA
                     GDRKVRYEEVLKVMDILQQGQVKRVGLLARPAP"
     gene            complement(2826..3515)
                     /gene="tolQ"
                     /locus_tag="VFV55_07395"
     CDS             complement(2826..3515)
                     /gene="tolQ"
                     /locus_tag="VFV55_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX4944163.1"
                     /translation="MIVSHDLTILAMIINASIVVKAVMTILVLASLFSWTYIFMKVFA
                     LRRAHRQAESFEREFWGGTDLVGLYQRAAGGRYVAAGMERIFEAGFKEYLKLKGRAGA
                     DVTALMDGTRRAMKATLQRELDTLESHLSFLATVGSVSPYIGLFGTVWGIMNAFRGLS
                     NVAQATLAQVAPGIAEALVATAIGLFAAIPAVIAYNRFAHDIDRLAGRYESFMEELTN
                     ILQRQAHQPER"
     gene            complement(3532..3975)
                     /gene="ybgC"
                     /locus_tag="VFV55_07400"
     CDS             complement(3532..3975)
                     /gene="ybgC"
                     /locus_tag="VFV55_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX4944164.1"
                     /translation="MTKIAVKPRLQPELFVYSFPVRVYFENTDAGGVVYHAEYLKFLE
                     RARTEWLRHLGFDHQALARNHRIVFVVTQAVVDFVKPARLDDHLAVSVQLESLGKVRC
                     VFAQEIRRDNEPLVRAKITVACVTGDAPKPAEIPEALRRKMQATL"
     gene            complement(3972..5003)
                     /gene="ruvB"
                     /locus_tag="VFV55_07405"
     CDS             complement(3972..5003)
                     /gene="ruvB"
                     /locus_tag="VFV55_07405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX4944165.1"
                     /translation="MIETDRLVTGDPASPQEEGEDRALRPRKLSEYVGQAKIREQLEI
                     FISAARNRGEALDHALLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAAIL
                     TNLEPRDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPGARSVKLDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIVARLEFYSPEELTRIVTRSSGLLAMAIEAEGAIEIARRSR
                     GTPRIANRLLRRVRDFAEVKYEGRVTAEIADLALRMLDVDVIGLDLLDRKLLLAIVEK
                     FGGGPVGVDNLAAAIGEERDTIEDVLEPFLIQQGYLQRTPRGRMATALTYQHFGLPGG
                     PAPGGGLFG"
     gene            complement(5000..5572)
                     /gene="ruvA"
                     /locus_tag="VFV55_07410"
     CDS             complement(5000..5572)
                     /gene="ruvA"
                     /locus_tag="VFV55_07410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX4944166.1"
                     /translation="MIGRIAGTLVEKNPPQVVVVAHGVGYEIDVPMSTFYNLPRTGEA
                     VELLTHLVVREDAHLLFGFNTAGERSAFRQLLRVSGVGPKVALSVLSGLSVDDLAAAV
                     ASEDVARLTRVPGIGKKTAERLILELRDKLPSALPAAKASAPHGPDVLNALLALGYNS
                     REASVAVRDLSPDLALTDAIRQALKVLAKP"
     gene            complement(5569..6090)
                     /gene="ruvC"
                     /locus_tag="VFV55_07415"
     CDS             complement(5569..6090)
                     /gene="ruvC"
                     /locus_tag="VFV55_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX4944167.1"
                     /translation="MRILGIDPGLRVTGFGVINREGQRLTYVTSGCIRTRDKGELPER
                     IRTILDGLAEVIAAHGPEHVAIEKVFVNVNPQSTLLLGQARGAAVCAAVLADLPVAEY
                     TALQVKQAVVGKGHARKEQVQEMVKRLLKLSGYPSADAADALACAICHANGGLGLGAL
                     PTKGLRMRDGRLA"
     gene            complement(6105..6836)
                     /locus_tag="VFV55_07420"
     CDS             complement(6105..6836)
                     /locus_tag="VFV55_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4944168.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKIFTRLIKEVTVAARMGGGDPD
                     MNPRLRLAVDKAYESNMPKDNVERAIKRGTGDLEGVNYEEVRYEGYGIGGAAVIVDCL
                     TDNRTRTVADVRHAFAKYGGNLGTDGSVAFLFKHCGLLIYAPGTSEDKLLDAALEAGA
                     EDVVTNDDGSIEVITGPHEFPAVKKALEKAGFKAEVAEVTMKPAAEAALAGEDAARMQ
                     KLVEALESLDDVQEVYTTAAMDEAP"
     gene            6914..>7029
                     /locus_tag="VFV55_07425"
     CDS             6914..>7029
                     /locus_tag="VFV55_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX4944169.1"
                     /translation="MLPPLVVAKSGVELGLLPGMANRHGLIAGATGTGKTVTL"
BASE COUNT          981 a         2401 c         2410 g         1237 t
ORIGIN      
        1 gtggtggtcg ccgttggcgg cttcgtcggc tgctgggtcg atgtggtgga ctggtcgaca
       61 acgggaacgt ccttcttgac ctcctggctg ctgcaggcgg cgacgaaggt gaccaacgca
      121 atgctcatga cgattttctt cacggatgac tccttcgaag tgatgcgcac gtaaacacgt
      181 aaagcggaaa gcggggattc tgcaccccgc tcaattcgta ggcaacggtc cccacgcggg
      241 atcgcgcacg tcgccggaag acgacacgag gcgctggcga acgcggccgt cgctcgagac
      301 ggcggcgagc tggccgcgtc cgccggtctg cgtctcgtag aggatcatcg ttccgttcgg
      361 cgcgtagctc ggcgaatcgt ccagcgtgcc gtcggtgagg acggtcacct ggccggtgcc
      421 gatctcgagc accgcgatgc ggaagcggcc cgcctcgcgc tgcacgaaag cgatcgactt
      481 tccgtcgggg ctgtaacgcg ggcgcgcgtt gtaagtaccc tcgaaggtaa gccgttgcgc
      541 gggcccgcca tccgcgggca tccggtagat ctgcggcgag cctccgcggt cggaggtgaa
      601 tgcgatccac ttgccgtcgg gcgagaagct gggttcggta tcgatggcgg gcgtgaccgt
      661 caggcggcgc ggctcgccgc cgccggccgg gatcacgtag atctgcgcga tgtcgtcctt
      721 cgtgagggtc accgccagct tcgacccgtc gggggaccac gccggcgcac tgttgttgcc
      781 gcggtagttc gcgagaatgc gcgtcgtgcc ctgcgcgagg ttctgcatca ccaccaccgg
      841 tttcttctgg tcgaaggaaa cgtaggcgat gcggctgccg tccggcgacc acgcgggcga
      901 gatgatgggc tcgttggagg cgagcaccgc ctgcgcgttt gcgccatcgg cgtccgacac
      961 ctgcagttcg aagcgcgtgc cgcgcttgac cacgtacgtg atcttggtgg aaaagacgcc
     1021 cttctcgccg gtgagctttt cgtagatgac gtcggcgatg cggtgggccg ttgcgcgcag
     1081 ctgcgcgggt gacacgacgt aggaatagct cgcaagctgc gactgcttct gcacgtcgaa
     1141 gagccggaag cgcacttcga cccggccatc ggcctgcgcc tcgaccttgc cgatcaccag
     1201 cgcctccgag gaacgcgcct gccagtcgac gaaattgacc tcggaaggct cggtgggcag
     1261 cgggctcact cccacggcgt tcaccagcct gaagaggccg gaccgcgcga ggtccgagcc
     1321 gacgaccgtg ctcaccacct gcggctgcgc ggtctcgccc gcgaagggca ggatcgcgat
     1381 cgggatctgg cggccgcccg atgtcgtgat gtcgatcgtg agctgcgcgc gcgcggcgag
     1441 cgcgacacag gcaaccagga tggcgaggat gattctcttc atgcggtcgg gtctctgcgg
     1501 gcgcggggcc cggctagcgt tcgtgttcga agttaagtat caggtcgcgg aatgtgggga
     1561 aaagttccga actcgccggc ggaaccggca acggcgaggc gctccggatc gcgcgcagga
     1621 tggcgtcgtc gtaggccggg ttggtgctcg ggcgcacgat ccgcgcgtcg agcacctcgc
     1681 ctccgggcag gatggtgatg cgcacctgca cctcgggctt gccgcgcacg gtgtcgggaa
     1741 cgttggcctt gccgcgtatc ttatcccgga tacgccctcg gtatctgtcg atctccgaaa
     1801 gacgcgcctg cgtggccgct tccgcggcgg atttcgcctg ggcctcggct tccgctttcg
     1861 cccttgcttc ggccaccgcc ttcgccttcg cgtcggcaac cgctttcgcc ttcgcatcgg
     1921 ccacggcctt cgcctcggcg gccgcctttg cctcgcgtgc cttcgtctcc tggaccttcc
     1981 tgtcgtgctc cctcttttcc agttccctct tttcctgctc cttcttcgcc cgttccttct
     2041 tctcccgctc cttcttctcg gcaatcgcag ggtcgggctt cggcggttcc ggcctgggtt
     2101 cggccttggg cggctcgggt ttcggcggtt ccggcttggg ttcggccctg ggcggttcgg
     2161 gtttcggcgg ctccgcttgc ggtggctcca ctttcggcgg ctgcggctcg gccttcggtg
     2221 ccgcgcccgg cttgcccgtc ggcagcttgt cccacagttc cgcctggagc ggcggcgtgg
     2281 gccggctctg ccacgtgacg ccgaagacga tcagcgcgaa gaacgccgcg tgcacggcga
     2341 ccgcgaggat gatcgaacgg atcttgcccg gctggcgggg cttttcctgc gactcgggcc
     2401 tcgccatcac ggcaccgcta gggcgcgggg cgcgcgagga ggcccacgcg cttcacctgc
     2461 ccctgctgca ggatgtccat caccttgagg acctcctcgt agcgcacctt gcggtcgccg
     2521 gcgatcacca ccgcctgctc cgcgttctcc cgttgcgcgc ggcgcacgat ctcgaggagc
     2581 tgcggcctct tcacgaggag ttcgtcgggg gagcgcgagc ggtccacgat ggacagctcg
     2641 ccgttttcgc ggatgcgcac ctcgaggggc gcgatggccg gatcgagctt cgcgcccacc
     2701 tggggcaggt cgatctggcc cgggttgatg agcggggcgg cgaccatgaa gatgatgagc
     2761 agcaccagca tcacgtcgat gtagggcacg acgttgatct cgctcatcgc gcggcggcgg
     2821 cgcatctacc gctccggctg gtgggcctgg cgctgcagga tgttcgtgag ctcctccatg
     2881 aagctctcgt aacggcccgc gagccggtcg atgtcgtgcg cgaaccggtt gtaggcgatc
     2941 accgcgggaa tcgcggcgaa gaggccgatg gcggtggcca ccagggcctc cgcgatcccg
     3001 ggcgcaacct gcgccagcgt ggcctgcgcc acgttcgaca ggccgcggaa ggcattcatg
     3061 attccccaca ccgtgccgaa gagcccgatg tacgggctca cggagcccac ggtggcgagg
     3121 aacgacaggt gcgactcgag cgtgtccagc tcgcgctgca gcgtcgcctt catcgcgcgg
     3181 cgcgttccgt ccatcagcgc ggtcacgtcg gcgccggccc ggcccttcag cttcaggtac
     3241 tccttgaagc ccgcctcgaa gatgcgttcc atgcccgccg cgacatagcg cccgcccgcg
     3301 gcgcgctggt agaggccgac caggtcggtg ccgccccaga actcgcgctc gaaggattcc
     3361 gcctggcggt gggcccggcg cagcgcgaag accttcatga agatgtaggt ccaggagaag
     3421 agcgaggcga gcaccaggat cgtcatgacg gccttcacga cgatgctcgc gttgatgatc
     3481 atcgcgagga tggtgaggtc gtggctgacg atcatggtgt ccccggcttt cctagagtgt
     3541 ggcctgcatc ttgcggcgaa gggcttcggg aatctccgcg ggcttgggcg cgtcccccgt
     3601 cacgcacgcc acggtgatct tcgcgcgcac caggggctcg ttgtcgcggc ggatctcctg
     3661 cgcgaagacg cagcgcacct tgcccagcga ttcgagctgg acgctcaccg cgagatgatc
     3721 gtcgagccgc gcgggcttca cgaagtccac caccgcctgc gtgaccacga agacgatgcg
     3781 atggttcctc gccagcgcct ggtggtcgaa gccgaggtgg cgcaaccact cggtgcgcgc
     3841 gcgctcgagg aacttgaggt actcggcgtg gtacaccacc ccgcccgcgt ccgtgttctc
     3901 gaagtacacg cgaacgggaa acgaatagac gaacagctcc ggctgcaggc ggggcttcac
     3961 ggcgatcttc gtcacccgaa gagccctccg cccggcgccg ggcctcccgg cagcccgaaa
     4021 tgctggtagg tgagcgcggt ggccatgcgc ccgcgcggcg tcctctgcag gtagccctgc
     4081 tggatgagga acggctccag cacgtcctcg atggtgtcgc gctcctcgcc gatggccgcg
     4141 gccaggttgt ccacgccgac ggggccgccg ccgaacttct ccacgatggc gagaaggagc
     4201 ttgcggtcca ggaggtcgag cccgatcacg tccacgtcga gcatgcgcag cgcgaggtcg
     4261 gcgatttccg ccgtcacgcg gccctcgtac ttcacctcgg cgaagtcgcg cacgcggcgc
     4321 agcaggcggt tggcgatgcg cggcgtcccg cgcgagcggc gcgcgatctc gatcgcccct
     4381 tccgcctcga tcgccatcgc gagcaggccc gacgaccggg tgacgatccg cgtgagttcc
     4441 tcgggggaat agaactcgag ccgcgccacg atgccgaagc ggtcgcgcag cgggttggtc
     4501 agcatgccgg cgcgcgtggt cgccccgacg agggtgaagg gcggcaggtc gagcttcacg
     4561 ctcctcgcgc ccggcccctc gccgatcatg atgtcgatct ggtagtcctc gagcgcgggg
     4621 tagaggattt cctcgacgac ggggctcagc cggtggatct cgtcgatgaa gagcacgtcg
     4681 cgcggctcga ggttggtgag gatggccgcg aggtcgccgg ggcgctcgag tacagggccc
     4741 gaggtctggc gcaggttgac gcccatctcg cgggcgatga tgtgcgcgag cgtggtcttg
     4801 cccaggcccg gcgggccgaa gaggagcgca tggtcgagcg cctcgccgcg attgcgcgcc
     4861 gcggagatga agatctccag ctgctcgcgg atcttggcct ggccgacgta ctcggagagc
     4921 ttgcgcgggc gcagcgccct gtcctcgccc tcctcctgcg gcgaagccgg gtctccggtg
     4981 accaggcggt cggtttcgat cacggcttgg cgagcacctt gagtgcctgg cggatcgcgt
     5041 cggtgagcgc caggtcgggc gacaggtcgc ggacggcgac cgacgcctcg cgggaattat
     5101 agccaagggc cagcagcgcg ttgaggacgt cgggcccgtg cggggccgac gctttcgccg
     5161 cgggcagcgc cgacggcagc ttgtcgcgca attcgaggat cagccgctcc gccgtcttct
     5221 tgccgatgcc cggcacgcgg gtgaggcgcg ccacgtcctc cgaggccacg gcggccgcga
     5281 ggtcgtcgac cgagagtccc gagagcaccg agagcgccac cttggggccc acgccgctca
     5341 ccctgaggag ctggcggaac gcgctgcgct cgcccgccgt gttgaagccg aagagcaggt
     5401 gcgcgtcctc gcggaccacg aggtgcgtca ggagctcgac cgcttccccg gttcgcggca
     5461 ggttgtagaa cgtgctcatc ggcacgtcga tctcgtagcc cacgccgtgg gcgaccacca
     5521 ccacctgcgg cgggttcttc tcgacgagcg tcccggcgat gcgcccgatc atgcgagcct
     5581 cccgtcgcgc atcctgaggc ccttcgtcgg caatgccccc agcccgagcc cgccgttggc
     5641 atggcagatg gcgcaggcca gcgcgtcggc cgcgtcggca gacgggtaac cggaaagctt
     5701 gagcagccgt ttcaccattt cctgcacctg ttccttgcgc gcatgaccct tgccgacgac
     5761 ggcctgcttc acctggagcg ccgtgtactc cgcgacgggc aggtccgcca gcaccgcggc
     5821 gcagacggcg gcgcccctcg cctggccgag cagcagcgtc gattgcggat tcacgttcac
     5881 gaacaccttt tcgatggcca catgttccgg accgtgcgcg gcgatcactt ccgccaggcc
     5941 gtcgaggatc gtgcggatgc gttccggcag ttcccccttg tcgcgcgtgc ggatgcagcc
     6001 gctcgtcacg taggtcaggc gctggccctc gcggttgatc accccgaacc ccgtgacccg
     6061 caggcccgga tcgatgccga ggatgcgcaa gagccggggc ccccctaggg cgcctcgtcc
     6121 atggcggccg tcgtgtagac ctcctgcacg tcgtcgaggg actcgagcgc ttccacgagc
     6181 ttctgcatgc gcgccgcgtc ctccccggcc agcgccgctt ccgcggcggg cttcatcgtc
     6241 acttcggcca cctccgcctt gaatcccgcc ttctccagcg ccttcttcac ggccgggaat
     6301 tcgtgcgggc cggttatcac ctcgatcgag ccgtcgtcgt tggtcacgac gtcctcggcg
     6361 ccggcctcga gcgccgcgtc gagcagcttg tcctcgctgg tgcccggcgc gtagatgagc
     6421 agcccgcaat gcttgaacag gaacgcgacg gagccatcgg tgccgaggtt gccgccgtac
     6481 ttcgcgaacg cgtggcgcac gtcggccacc gtgcgcgtgc ggttgtccgt gaggcagtcc
     6541 acgatcaccg cggcgccgcc gatcccgtag ccctcgtagc ggacctcctc gtagttcacc
     6601 ccctccaggt caccggtgcc gcgcttgatg gcgcgctcga cgttgtcctt gggcatgttg
     6661 ctttcgtacg ccttgtcgac ggcgaggcgc agccgcgggt tcatgtcggg atccccgccg
     6721 cccatccgcg cggcgaccgt gacttccttg atgaggcggg tgaagatctt gccgcgcttg
     6781 gcgtcctggc gacccttgcg gtgctggatg ttcgcccact tgctgtgtcc cgccatgggt
     6841 cttcgtcgcg aattcagttt cgaaggggct aatggtaaca tcctcgctac gtcttcccgg
     6901 ccgagcccat cccgtgctgc ccccccttgt cgtcgcgaaa tccggcgttg aactgggcct
     6961 tctgccgggc atggccaatc gccatggcct catcgccggc gcgaccggaa ccggcaagac
     7021 cgtcaccct
//
LOCUS       DASUEV010000229        13323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_289000, whole genome shotgun sequence.
ACCESSION   DASUEV010000229 DASUEV010000000
VERSION     DASUEV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13323
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289000"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..510)
                     /locus_tag="VFV55_07430"
     CDS             complement(76..510)
                     /locus_tag="VFV55_07430"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX4944170.1"
                     /translation="MRAAAALIAGFLALPAPAAEFRTVGDHVAILYDAPSLKADRVFV
                     ASRGYPFEVLVKLDLWTKVRDAGGEVAWAENRSLGAQRNALVSVPVADVRAAPDAKSA
                     LVFEAYKQVILEVLEPPANGWVKVRHRDGQQGYARVAHLWGV"
     gene            complement(519..992)
                     /gene="secB"
                     /locus_tag="VFV55_07435"
     CDS             complement(519..992)
                     /gene="secB"
                     /locus_tag="VFV55_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX4944171.1"
                     /translation="MSDAQNQPVFSIEKLYVKDVSLEIPNAPQVFLEREGPQVDIQLH
                     HESAKVDDGVYQTVLTVTVTAKVKDKTLFLVEVGQAGIFVIRNIPDADMDPVLGIACP
                     NILYPYVREVVSDMVSRAGFPPVILTPVNFEALFQAQKQAQAEQAPASPIIQATH"
     gene            complement(1033..1290)
                     /gene="grxC"
                     /locus_tag="VFV55_07440"
     CDS             complement(1033..1290)
                     /gene="grxC"
                     /locus_tag="VFV55_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX4944172.1"
                     /translation="MARIVMYATGVCPYCVMAEKLLRSKGVVEIEKVRVDLDPARREE
                     MMQRTGRRTVPQIYIGERHVGGFDDLAALDHAGSLDSLLAA"
     gene            complement(1295..1708)
                     /locus_tag="VFV55_07445"
     CDS             complement(1295..1708)
                     /locus_tag="VFV55_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX4944173.1"
                     /translation="MGEFLLNNLALVGLFLASGALLLWPEISRFAGGASSVGTLDATR
                     LMNQPGALVLDVRETAEFAAGHLPRARHIPLGELGKRLDELARFKDKPVIVTCRSGSR
                     SGSACRTLRNAGFSNVHNLKGGVPAWEQASLPVER"
     gene            complement(1773..2090)
                     /locus_tag="VFV55_07450"
     CDS             complement(1773..2090)
                     /locus_tag="VFV55_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX4944174.1"
                     /translation="MNAWTVIIGAGIVALLLRASFIVFADPHKFPHGFRVALGYVPPA
                     VLAAIVVPGLAMPHGELDLSLANPRLLAGVAAFAIAARYRGTLAPIAIGMVVLWVLQA
                     ALG"
     gene            complement(2087..2794)
                     /locus_tag="VFV55_07455"
     CDS             complement(2087..2794)
                     /locus_tag="VFV55_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX4944175.1"
                     /translation="MLPSSHPPEASREFIAGMREIAPALLGTMPFGFVAGVAAVGAGM
                     NPLEAIALSIIVFSGIAQLVVAQLVAAQAPVFVILAAVTVVSLRHLMYSAALSPHLAH
                     LPARWRLLLAYLMTDQSFALGVKRFGEPGERAQRHFYVLGVASTLFVAWQFSVALGAL
                     AGAQVPANWSLDFVVTLTFLGLLVPALRTRADIAAATVASATALVAAGLPYKLALVAA
                     SLAGIGAGVAAERAGRR"
     gene            2897..3640
                     /gene="gpmA"
                     /locus_tag="VFV55_07460"
     CDS             2897..3640
                     /gene="gpmA"
                     /locus_tag="VFV55_07460"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX4944176.1"
                     /translation="MNKLVLLRHGESLWNKENRFTGWTDVDLSAQGVEEARAAGRLLA
                     AQGYDFDVAFTSVLTRAIRTLNLALEEMDRLWLPVEKDWRLNERHYGALQGLNKAEIA
                     GKFGEAQVLAWRRSYDTPPPELMPGDERDAARDRRYAGLGSRVPRTECLKDTVARVVP
                     AWHERISPRVAAGDRVLVAAHGNSLRALVKFLDDISDTDIVALNIPTGVPLVYELDDV
                     MKPIRHYYLGDPEAVAKAAAAVASQGKAR"
     gene            3644..4912
                     /locus_tag="VFV55_07465"
     CDS             3644..4912
                     /locus_tag="VFV55_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX4944177.1"
                     /translation="MQARRLAAAFVTGLAFATCLASAGAEARGKAAPAAASEEDLRQL
                     RARIERLEKDLSQTEESRGEAADALKASEKAVSEANRALFELANAHRALSAQLAQLGE
                     RGARARDAIAGQQALAERLLRLQYEQGGQDRLRLILEGRDLTIVTRHLAYFGYVQRAR
                     AESLAALKRGAEEVAALEAEARQKREAIAENQAAQARESQRLERERAGRAAALKRLAG
                     QVAKGKREIGRLKRDEERLTRLVQEIARALATREAQKSRDAQKAKDRDAGKAAVNRRR
                     GTPVDQVADASLSARAFPSLKGRLKLPVRGELMNRYGGPREEGGTTWRGLFIRAAVGE
                     VVRAVADGRVVYADWLRGFGNLLILDHGGGYMSLYGYNDGLLRQVGEGVKGGEEVAQV
                     GASGGAEESGLYFELRYDGKPFDPLRWVAR"
     gene            4984..6441
                     /locus_tag="VFV55_07470"
     CDS             4984..6441
                     /locus_tag="VFV55_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX4944178.1"
                     /translation="MSSKLQQVGLVVLGAALGIAASLHLSAIADRQAAVVQLPVDDLR
                     LFSEVFGRIKNDYVEPVEDKKLLKEAINGMLTGLDPHSAFLDQDAYKDLQVGTQGEFG
                     GLGIEVGAEDGFVKVVAPIEDTPAHRAGVQAGDLIIKLDGVSLKGMSLNDAVKRMRGK
                     PHSEVTITVMRKGVPAPIDIKIRRDVIRVKSVKSRMIEPGYGMVRVTQFQEHTGENLV
                     TAVNDLYKQSNGDLKGLVLDLRMNPGGLLNAAVAVSSAFLPKNALVVYTDGRTDDAKM
                     RLTANKENYVRGTFREDYLARLPAAAKAVPLVVLVNGASASASEIVAGALQDHKRATV
                     IGTQTFGKGSVQTILPLPNNQAVKLTTARYFTPNGRSIQAKGIVPDIVVEDATTDSRI
                     FMREADLQHHLAGDGEKGKDDAAKPAETPVPVNPALTKPAPAKPAPAKPAPESKATAT
                     PDAAAAADGGKKEDVQLAAAMKFLKGLPITAAVAK"
     gene            6464..7228
                     /gene="moeB"
                     /locus_tag="VFV55_07475"
     CDS             6464..7228
                     /gene="moeB"
                     /locus_tag="VFV55_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX4944179.1"
                     /translation="MDDRQRLRYSRHLLLDGFGEDSQERLLASRALVVGAGGLGSGAL
                     LYLASSGVGHITVADGDTVDLTNLQRQVVHRADAVGTNKAVSAAATLATINPEIAVDV
                     LPERLTPERLRVLAGEADVVLDCSDNFATRHAINEACVRSRVPLVSGAGIRFDGQVAV
                     FDHRQPGSACYHCLFPAGAQGDEERCATMGVFAPLVGVIGTMQAMEAIKLLAGVGETL
                     AGRLLLFDARATRWHEVRLSRDPQCAVCGEPRAAAA"
     gene            7239..7754
                     /locus_tag="VFV55_07480"
     CDS             7239..7754
                     /locus_tag="VFV55_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4944180.1"
                     /translation="MEESVRLLAILAPSLIAATLALAGPDKQVFWKLVDSKGKVTYSD
                     KAPPGNHGGKVTRIEVDLAANRAILVNVGESSPPVQLPLTAPEMKRVKADAELARARE
                     NLEAARKARDDGKDPTAEETQWVGKVGGGARPVPNEAYGARIKKLDDAVKHAQDEFER
                     ASKAARMAGID"
     gene            7747..8334
                     /locus_tag="VFV55_07485"
     CDS             7747..8334
                     /locus_tag="VFV55_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX4944181.1"
                     /translation="MTERPVVRALLLALAATVAWPAAAQVLYKLTDRQGRVTYSDSEP
                     RNFDGTVVRLEADTAANIMPSAKKGESAPRPGPDSGIGEDRRHVREDLEKKLRAAQAR
                     VDAVRKAKAEGGEPLPEEMQTIQHRRAPLGPGQAPPNPNCFAAVDPNGAASLNCPTRV
                     PLDAFYQRQKQLEEELRRAEEELALAERAYRRGTD"
     gene            complement(8369..9382)
                     /gene="tsaD"
                     /locus_tag="VFV55_07490"
     CDS             complement(8369..9382)
                     /gene="tsaD"
                     /locus_tag="VFV55_07490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX4944182.1"
                     /translation="MLTLGIETSCDETGIALYDSERGVLGEALHSQVDLHARYGGVVP
                     ELASRDHIRRVVPLVREALAQAGRGLGDVDLIAYTQGPGLAGALLVGASVASGLGIAL
                     GRPVVGIHHLEGHLLSPLLADPRPAFPFVALLVSGGHTQLMAVEGVGRYALLGETVDD
                     AAGEAFDKTASLLGLAYPGGPALANLALGGDGKRFAFPRPMMASEDLDFSFSGLKTAA
                     LLCVRKHPLDEQLRADIAASFQAAVIDVLASKCLRALEMTGMDRLVVAGGVGANAALR
                     DRLLRETARAGASVFFPPPRLCTDNGAMIAFAASMRYRDAPAGAEDFTVRPRWELAAI
                     ASA"
     gene            9472..9684
                     /gene="rpsU"
                     /locus_tag="VFV55_07495"
     CDS             9472..9684
                     /gene="rpsU"
                     /locus_tag="VFV55_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX4944183.1"
                     /translation="MPTIRVKENEPFDVALRRFKRAIEKTGLLTELRAREFYEKPTAE
                     RKRKHAAAVKRRFKRMRSQMLPPKLY"
     gene            9761..10207
                     /locus_tag="VFV55_07500"
     CDS             9761..10207
                     /locus_tag="VFV55_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX4944184.1"
                     /translation="MLLRERVMEDVKAAMKAREAERLGTLRLLTAAMKQREVDERITL
                     DDAGIVAVIEKMLKQRRDSVAQYELAGRQDLADVEKREMAILQAYLPQQLTEAEIAAI
                     VADAVAATGAKAPSDMGRVMAIVKPGVAGRADMGRVSALVKAKLAG"
     gene            10284..12101
                     /gene="dnaG"
                     /locus_tag="VFV55_07505"
     CDS             10284..12101
                     /gene="dnaG"
                     /locus_tag="VFV55_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX4944185.1"
                     /translation="MIPQDFIQTLLGRVDIVDVIDRHVRLKKAGANFKACCPFHNEKT
                     PSFNVSPAKQFYHCFGCGVHGNAIGFVMEYSGLTYPDAIRELAQQAGMEVPEVQGTVP
                     GRPAAAQAKGLTDRMMEALNYYRAELKRTPEAIEYLKGRGVSGEIAARFGLGFAPDAW
                     QSLKGVFADYESAGMKDTGLVIDSEGGEGHEGEPAKKARRYDRFRGRVMFPILDSRGN
                     VIGFGGRVLGDGEPKYLNSPETPLFEKGRELYGLWQARRAIRDANLVIVVEGYMDVVA
                     LAQHGVENAVATLGTATTPMHVAKLLKLADSVVFCFDGDSAGRKAAWKALEVSLPVLA
                     DGKSVAFLFLPQEDDPDTFVRRLGREAFLEALAGAKPLSQYLVSEIAAKVDMRSEEGR
                     ARFLAQAKPLVSQIEAPALGAMIRRRLAELAGLDPGELQGYAPQRDSWRPRPAPAARV
                     VRRTHSLEAQILQRVLRKPDLARSVDPDLVSDGSAEGRALHALLAFVREGEAGLSLGQ
                     VIAHFDGSEHGPALDAAIAGGSILDEVPEAELDLAAELAGLQDKLGMQRSERRKAELE
                     MRSMAAGLTGAEQAELAGLKARQAAAKGVNSALELPPKQ"
     gene            12149..>13323
                     /locus_tag="VFV55_07510"
     CDS             12149..>13323
                     /locus_tag="VFV55_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEX4944186.1"
                     /translation="MKSSTRDSQKNAKDSQKNASKDQHKAEVKLAEVEAQKKRLKNLI
                     VLGKERGYLTYAEINDHLPDEMTDAEQIESVVTMLADMGIAVYDQAPDAETLLLTEAA
                     TNPVADEDVVEEAEEALSLTVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIE
                     DGLKHMIHAISSCPTTIEEILRLAELVEKDEMRIDELVDGLVDADGEDIVGEEMSEEE
                     ELEEIEEDEAEEDADMASADLEQLKNDSLVHFNKIRRLYKKMRKILAEKGYRSRAYKD
                     LQESVSGELLMVRFTAKQVEHLCGGLRQLVERVRGHEREIMELCTRNSSMPRQHFIKV
                     FPGNETNLKWVAEEISSGKAFAKALERFKPAIVEQQEALISLQKEVGIPIKDLKEIAR
                     "
BASE COUNT         2317 a         4457 c         4720 g         1829 t
ORIGIN      
        1 ccggaagcgg aaagggaaat cgccagcgcc gaaccccaag ctcccgcgcc caggacggcg
       61 atcctcatcg aagccctaca cgccccagag gtgggcgacg cgcgcgtaac cctgctgccc
      121 gtcgcggtgg cgcaccttca cccagccatt ggccggaggt tccagcacct cgaggatcac
      181 ctgcttgtag gcctcgaaga cgagcgcgga cttggcgtcg ggcgcggcgc gcacgtcggc
      241 cacgggcacg ctcaccagcg cgttgcgctg cgctccgagc gacctgttct ctgcccacgc
      301 cacctcgccg ccggcgtccc gcaccttcgt ccagaggtcc agcttcacca gaacctcgaa
      361 gggatagccc ctgctcgcga cgaagacccg gtccgccttg agcgacggcg cgtcgtagag
      421 gatcgcgacg tgatcgccca ccgtgcggaa ctcggcggcg ggcgcgggaa gcgccaggaa
      481 ccccgcgata agggccgccg cggcgcgcat cggtatcgtc agtgcgtggc ctggatgatc
      541 gggctggccg gcgcctgctc cgcctgcgcc tgcttctgcg cctggaaaag cgcctcgaag
      601 ttgaccggcg tcaggatcac gggcgggaat ccggcgcgcg agaccatgtc ggagaccacc
      661 tcgcggacgt aggggtagag gatgttcggg caggcgatgc cgagcaccgg gtccatgtcc
      721 gcgtccggga tgttgcggat cacgaagatc ccggcctggc ccacctcgac gagaaagagc
      781 gtcttgtcct tgaccttcgc ggtcaccgtg acggtgagga ccgtctggta cacgccgtcg
      841 tcgaccttgg ccgattcgtg gtgcagctgg atgtcgacct gcggcccctc gcgctcgagg
      901 aacacctgcg gggcgttggg aatctctagc gagacgtcct tcacgtagag cttctcgatc
      961 gaaaagacgg gctggttctg ggcgtcgctc atggtgcttc cttcggttgg gtttcggtgt
     1021 ggcgcgctgc cgtcaggcgg cgagcaggga atcgaggctg ccggcatggt cgagcgcggc
     1081 aaggtcgtcg aagccgccga cgtggcgctc tccgatgtag atctgcggaa cggtgcgccg
     1141 gccggtgcgc tgcatcattt cctcgcggcg cgcggggtcg aggtcgacgc ggaccttctc
     1201 gatctcgacc acgcccttgg aacgcaggag cttctcggcc atgacgcaat acgggcacac
     1261 gccggtcgcg tacatcacga tcctggccat cgccctagcg ttccacggga aggctcgcct
     1321 gctcccaggc cgggacgcct cccttgaggt tgtgcacgtt cgagaagccc gcattcctca
     1381 gcgtccggca tgcggatccg gagcggctgc cgctgcggca ggtgacgatc accggcttgt
     1441 ccttgaacct ggcgagttcg tccaggcgct tgccgagttc gcccagggga atgtggcgtg
     1501 cgcgcggcag gtggccggcg gcgaattccg cagtctcgcg cacgtcgagc accagtgcgc
     1561 ccggctggtt catcaggcgg gtcgcgtcga gcgtgcccac ggagctggcg ccgcccgcga
     1621 agcgcgagat ctcgggccag aggaggagcg cgccgctcgc gaggaacagg ccgacgaggg
     1681 caaggttgtt gagcaggaat tcgcccatgg cgggagtatt caggttggca aaagatcgat
     1741 tttatcagcg gcgggcctgc gcgggggcgc gatcagccga gcgcggcctg caacacccac
     1801 aggacgacca tgccgatggc gatgggcgcc agcgttccac ggtacctcgc ggcgatggcg
     1861 aaggctgcca cgcccgcgag cagccgcgga ttggcgagcg aaaggtcgag ctcgccgtgg
     1921 ggcatcgcga ggccgggaac gacgattgcg gcaagcaccg cgggcggcac gtagccgagg
     1981 gcaacgcgga acccgtgcgg gaacttgtgc gggtcggcga acacgatgaa ggacgcgcgc
     2041 agcaggaggg ccacgattcc cgcgccgatg attaccgtcc aggcattcat cgccggccgg
     2101 ccctttcggc cgccacgccg gcgccgattc ccgcgagcga agcggccacg agcgccagct
     2161 tgtacggcag cccggcggca accagcgccg ttgcggaggc cacggtcgcc gcggcgatgt
     2221 ccgcgcgcgt gcgcagtgcg ggaaccagca gtccgaggaa ggtgagggtg accacgaaat
     2281 cgagcgacca gttcgccggc acctgggcac ccgcgagcgc gccgagggcg accgaaaatt
     2341 gccaggcgac gaagagggtc gatgcgactc cgaggacgta gaagtgccgc tgcgcccgct
     2401 cgcccggctc gccgaaccgc ttcacgccca gcgcgaagct ctggtcggtc atcaggtacg
     2461 cgaggagcag ccgccacctc gcgggcaggt gcgcgaggtg gggcgacagg gccgcgctgt
     2521 acatcaagtg gcgcaggctc acgacggtca cggccgcgag tatcacgaag acgggcgcct
     2581 gcgcggccac gagctgcgcc acgaccagtt gcgcgatgcc ggaaaagacg atgatggaga
     2641 gcgcgattgc ctcgagcgga ttcattcccg cgcccaccgc cgccacgccc gcaacgaagc
     2701 cgaacggcat ggtgccgagc agcgcgggag cgatctccct catgcccgcg atgaattcgc
     2761 ggctggcctc gggcggatgg gacgaaggaa gcacggaagg taggcgggga gggagcccgg
     2821 cagtgtagcg gagagcgcgg agcgggcgcg agcgtttgct accatacgtc tgccctttcc
     2881 ctgcctgccg atccccatga acaagctcgt cctcctccgc catggcgaaa gcctctggaa
     2941 caaggaaaac cgtttcaccg gctggacgga tgtcgacctg tcggcgcagg gcgtggagga
     3001 agcgcgcgcc gccggccgcc tcctcgcggc gcagggctac gacttcgacg tcgccttcac
     3061 ctcggtactc acgcgcgcaa tccgcacgct caacctcgcg ctcgaggaga tggatcgcct
     3121 ctggcttccg gtggagaagg actggcgcct gaacgagcgc cattacggcg ccctccaggg
     3181 gctcaacaag gccgagatcg ccggcaagtt cggcgaggcg caggtgctcg cctggcgtcg
     3241 cagctacgac acgccgccgc ccgagctgat gcccggcgac gagcgcgacg ccgcgcgcga
     3301 ccggcgctat gcaggcctgg gttcgcgcgt gccccgaacc gaatgcctca aggacaccgt
     3361 ggcgcgcgtg gtgcccgcct ggcacgagcg catctccccg cgcgtggcgg cgggcgaccg
     3421 ggtgctggtc gccgcgcacg gcaacagcct gcgcgcgctg gtgaagttcc tcgacgacat
     3481 ctccgacacg gatatcgtcg cgctcaacat tcccacgggc gtgccgctcg tctacgagct
     3541 cgacgacgtg atgaagccca tccgccacta ctaccttggc gacccggagg cggtggcgaa
     3601 ggcggccgcg gcggtcgcga gccagggcaa ggcccgctga ggaatgcagg cgcggcgcct
     3661 cgcggccgcc ttcgtcaccg gccttgcctt cgcgacgtgc ctcgcatcgg cgggggccga
     3721 ggctcgcggg aaggccgcac cagccgcggc ttccgaggag gatctccggc agttgcgcgc
     3781 gcgcatcgag cggctggaga aggacctttc gcaaacggag gaatcgcgcg gcgaggccgc
     3841 cgatgcgctc aaggcctcgg aaaaggcggt gagcgaggcc aaccgcgcgc ttttcgaact
     3901 cgcgaatgcg caccgggcgc tgtcggcgca actcgcgcag cttggcgaac gcggcgcgcg
     3961 ggcgcgcgac gcgatcgcgg gccagcaggc gctcgccgaa agactcctgc ggctgcaata
     4021 cgagcagggc ggccaggacc gcctgcggct catcctcgaa gggcgcgacc tcaccatcgt
     4081 cacgcgccac ctcgcctact tcggttacgt gcagcgtgcg cgcgccgagt cgctcgccgc
     4141 gctcaagcgc ggagccgagg aggtggcagc gctggaagcc gaggcccggc agaagcgcga
     4201 ggcgatcgcc gagaaccagg cggcgcaggc tcgcgaatcg cagcgcctcg agagggagcg
     4261 cgccgggcgg gccgcggccc tgaagcggct ggccggccag gtcgcgaagg gcaagcgcga
     4321 gatcggccgg ctcaagcggg acgaggaacg gctcacgcgc ctggtgcagg aaatcgcccg
     4381 ggcgctggcg acgcgcgaag cgcagaaatc gcgcgacgcc cagaaagcga aggatcgcga
     4441 cgccggcaag gctgcggtga atcgtcgcag gggaacgccc gtggaccagg tcgcggatgc
     4501 ttcgctctcg gcgcgggcgt ttccgagcct gaaggggcgc ctgaagctgc cggtgagggg
     4561 ggaactcatg aaccgttacg ggggtccacg tgaagagggg ggcacgacct ggcgcggcct
     4621 gttcatccgg gccgcggtag gggaggtggt ccgggccgtg gccgacgggc gggtcgtcta
     4681 cgcggattgg ctgcgcggct tcggcaatct cctcatcctc gaccacggtg gtggctacat
     4741 gagcctgtac ggctacaacg acgggctgct tcgccaggtc ggggagggcg tcaagggtgg
     4801 tgaagaagtg gcgcaggtgg gggcctcggg gggcgccgag gaatcgggtt tatacttcga
     4861 attgagatac gacgggaaac cgttcgaccc gttgcgctgg gtggcgcgct gaggtctccg
     4921 gaagcgggga tcgcgcgcaa cccggctatg tcgttgtcct ggatttcggc gaggtggcga
     4981 atcatgagca gcaaacttca gcaggtcggc ttggtggtcc tcggggccgc gctcgggatt
     5041 gccgcgagcc tgcatttgtc cgcgatcgcc gaccggcagg ccgccgtggt gcagttgccc
     5101 gtcgacgacc ttcgcctctt ctccgaggtg ttcgggcgca tcaagaacga ctacgtcgag
     5161 ccggtcgagg acaagaagct cctcaaggaa gcgatcaacg ggatgctcac cgggctcgac
     5221 ccgcattccg ccttcctcga ccaggatgcc tacaaggatc tccaggtcgg aacccagggc
     5281 gaattcggcg gcctgggcat cgaggtggga gccgaggacg gcttcgtcaa ggtcgtcgcg
     5341 ccgatcgagg acacgccggc ccaccgcgcg ggcgtccagg cgggcgacct catcatcaag
     5401 ctcgacggcg tgagcctcaa gggcatgagc ctcaacgacg cggtgaagcg catgcgcggc
     5461 aagccccact ccgaggtcac gatcaccgtg atgcgcaagg gcgtgcctgc gccgatcgac
     5521 atcaagatca ggcgcgacgt gatccgcgtg aagagcgtga agtcgcggat gatcgaaccg
     5581 ggctacggga tggtgcgcgt cacgcaattc caggaacaca ccggcgagaa cctcgtgacg
     5641 gcggtgaacg acctctacaa gcagtcgaac ggcgacctga agggcctggt gctcgacctg
     5701 cgcatgaatc ccggcgggct gctgaacgcg gccgtcgccg tgtcatccgc gttcctcccg
     5761 aaaaatgcgc tcgtggtcta caccgacggg cgaaccgacg acgcgaagat gcgcctcacg
     5821 gcgaacaagg aaaactacgt tcgcgggacc ttccgcgagg actacctggc caggctgccg
     5881 gccgccgcga aggccgtgcc gctggtcgtc ctcgtgaacg gcgcctcggc ctcggcctcg
     5941 gagatcgtcg cgggcgcgct gcaggaccac aagcgcgcca cggtcatcgg cacccagacc
     6001 ttcggcaagg ggtcggtcca gacgatcctg ccgctgccca acaaccaggc ggtgaagctc
     6061 acgacggcgc gctatttcac gccgaacggc cggtccatac aggcgaaggg catcgtgccc
     6121 gacatcgtcg tcgaggacgc aacgaccgac tcgcgcatct tcatgcgcga ggccgacctg
     6181 cagcaccacc tcgccggcga cggcgagaag ggcaaggacg acgcggcgaa gcccgcggag
     6241 acgccggttc ccgtgaaccc ggcactcacg aagcccgcgc ccgcgaaacc cgcgcccgcg
     6301 aaaccggcac cggagtcgaa ggccaccgcg acgcccgacg cggcggccgc ggccgacggc
     6361 ggcaagaagg aagacgtcca gttggccgcc gcgatgaaat tcctcaaggg gctgccgatc
     6421 accgctgcgg tcgccaagta ggcgggaccc cgcgaggtgc gccgtggacg accgccaacg
     6481 gctgcgctat agccgccacc tcctgctcga cggattcggc gaggactcgc aggagcgcct
     6541 cctagcctcg cgtgcgctcg tcgtcggggc cggtggattg ggctcgggcg cgctgctgta
     6601 tctcgcctcg agcggcgtgg ggcacatcac cgtcgccgac ggcgacacgg tcgacctcac
     6661 caacctgcag cgccaggtgg tgcatcgcgc agacgccgtc ggcacgaaca aggcggtttc
     6721 ggccgccgcc acgctcgcga cgatcaatcc cgagattgcg gtcgatgtct tgcccgaacg
     6781 cctcacgccg gaacgccttc gagtgctggc tggagaggcg gacgtggtcc tcgactgttc
     6841 cgacaacttc gccactcgcc acgcgatcaa cgaggcttgc gtccgctcgc gcgtgccgct
     6901 cgtctcggga gcgggcatcc gcttcgacgg gcaagtcgcg gtcttcgatc atcgccagcc
     6961 cgggtcggcc tgttaccact gcctgttccc cgcgggagca cagggcgacg aagagcgatg
     7021 cgcgaccatg ggcgtgttcg cgccgctggt cggcgtgatc ggcacgatgc aggcgatgga
     7081 ggcgatcaag ctcctcgcgg gcgtgggcga aacgctcgcc ggccggctcc tcctcttcga
     7141 tgcgcgcgca acgcgctggc acgaggttcg cctttcgcgc gacccgcaat gcgccgtgtg
     7201 cggtgaaccg cgcgcggccg cggcctagga gcagatcgat ggaagaatcc gtgcgcctgc
     7261 tagcgatcct tgcgccttcg ctcatcgcgg ccacgctcgc cctggccgga ccggacaagc
     7321 aggtcttctg gaagctcgtc gacagcaagg gaaaggtcac ctattccgac aaggcaccgc
     7381 ccgggaatca tggcgggaag gtgacgcgga tagaggtcga cctcgccgcc aatcgcgcga
     7441 tactcgtcaa cgtgggcgag tcctccccgc cggtgcagct tcccctcacc gcgccggaaa
     7501 tgaagcgcgt gaaggccgat gcggagctgg cgcgtgcgcg cgagaacctc gaggcggcca
     7561 ggaaggcgag ggacgacggc aaggacccga cggcggaaga aacgcagtgg gtcggcaagg
     7621 tgggcggcgg cgcccgcccc gtgccaaacg aagcctacgg tgcgcgcatc aagaaactcg
     7681 acgacgcggt gaagcacgcg caggacgagt tcgagcgcgc cagcaaggcc gcccggatgg
     7741 ccggcattga ctgagcgccc ggtggtgcgt gcgttgctgc tggccctggc cgcgaccgtg
     7801 gcgtggcccg ctgccgcgca ggtgctctac aagctcaccg accgccaggg ccgcgtcacc
     7861 tattcggaca gcgagcccag gaacttcgat ggcaccgtgg tccgcctcga ggccgacacg
     7921 gccgccaaca tcatgccgtc cgcgaagaag ggcgagagcg cgccacgccc cggccccgat
     7981 tcgggaatcg gggaagatcg ccgccatgtc cgcgaggatc tcgagaagaa gctgcgcgcg
     8041 gcccaggcgc gcgtcgatgc cgtgcgcaag gcgaaagcgg agggcggcga gcccctgccc
     8101 gaggaaatgc agaccatcca gcatcgccgc gctccgctcg ggccggggca ggcgccgccg
     8161 aatccgaact gcttcgccgc cgtcgatccc aatggcgccg catcgctcaa ctgtccgacc
     8221 cgggtgccgc tcgacgcgtt ctaccagcgc cagaagcagc tcgaggagga gctcaggcgc
     8281 gccgaggagg aactggcgct cgccgagcgc gcctatcgcc gcggcacgga ctaggacggc
     8341 cggcagcggc aggagttgtc cgcggccatc aggcgcttgc gatggcggcg agctcccagc
     8401 gagggcggac cgtgaagtct tccgcgcccg ctggtgcatc gcggtagcgc atcgacgctg
     8461 cgaacgcgat catcgcgccg ttgtcggtgc agagtcgcgg cggcgggaag aagacgctgg
     8521 cgcccgcgcg ggcggtctcg cgaagcagcc ggtcgcgaag cgccgcattg gcacccacgc
     8581 cccccgcgac cacgagccgg tccatgccgg tcatctccag cgcgcgaagg cacttgctgg
     8641 ccaggacgtc gatgaccgcc gcctggaacg aggcggcgat atcggcccgc agctgctcgt
     8701 ccagcgggtg cttgcggacg caaagcaggg cggcggtctt caggccgctg aagctgaagt
     8761 cgagatcctc gctcgccatc atcggcctcg ggaaggcgaa acgcttcccg tcgccgccga
     8821 gggcaaggtt cgccagcgcc gggccgccgg gatacgcgag ccccagcagg cttgccgtct
     8881 tgtcgaaggc ttctccggcc gcgtcgtcca cggtctcgcc gagcagtgcg tagcggccaa
     8941 cgccttcaac ggccatgagc tgcgtgtggc cccccgagac cagcagcgcg acgaagggaa
     9001 acgccggccg cgggtccgcg agcagcggcg acaggagatg gccctcgagg tggtggatgc
     9061 cgaccaccgg cctgccgagc gcgatgccca gccccgaggc cacggaggca cctaccagga
     9121 gcgcgccggc aaggccggga ccctgggtgt acgcgatgag gtccacgtcg cccaggccgc
     9181 gcccggcttg cgcaagggcc tcccggacga gcggaaccac gcggcggatg tggtcgcgcg
     9241 aggcgagctc cggcaccacc cccccgtagc gggcgtgcag gtcgacctgc gaatgcaggg
     9301 cctcgccaag gacgccgcgc tcgctgtcgt agagcgcgat gccggtttcg tcgcaggaag
     9361 tctcgatgcc gagggtgagc acgggagagc ggcgccaccg gcgcctttcc tagaatgcaa
     9421 aactttgata ttatgcaggg ttttcagcgt tgcccaatca gcggaaccta catgccgacc
     9481 attcgagtca aggaaaacga gccgttcgac gttgcgctgc gccgtttcaa gcgcgcgatc
     9541 gagaagacgg gcctgctcac cgaactgcgc gcgcgcgagt tctacgagaa gcccacggcc
     9601 gagcgcaagc gcaagcacgc ggccgccgtg aagcgccgct tcaagcgcat gcgctcgcag
     9661 atgcttccgc cgaagctgta ctgaccggcc ccgggcaagt cattgcggcg agccgggccc
     9721 gagcgccccg ctcgccgcgg tcgtttccgg aaaggcaacc atgctgctgc gcgaacgggt
     9781 gatggaggac gtcaaggcgg ccatgaaggc gcgcgaggcc gagcgcctgg gcacgctgcg
     9841 cctgctcacg gcggccatga agcagcgcga ggtcgacgaa cggatcacgc tcgacgatgc
     9901 cggcatcgtc gccgtcatcg agaagatgct caagcagcgc cgggattcgg ttgcccagta
     9961 cgaattggcg gggcgccagg acctggccga cgtcgagaag cgggagatgg ccatcctgca
    10021 ggcttacctc ccgcagcaac tcaccgaggc cgagatcgcg gcaatcgtcg cggatgcggt
    10081 ggctgccacc ggggccaagg ctccctccga catgggccgg gtgatggcga tcgtgaagcc
    10141 cggggtagcc ggccgcgcgg acatgggccg cgtatcggcg ctggtgaagg cgaaactggc
    10201 aggctgaatc gccgcggggc cggcgttccc cgccgcccgc gggaagacga tccgcacggg
    10261 gaccgggagc gaaccttacc cgcatgatcc cgcaggactt catccagacg ctcctcgggc
    10321 gcgtcgacat cgtcgacgtg atcgatcgcc atgtccggct caagaaggcg ggcgcgaact
    10381 tcaaggcgtg ctgcccgttc cacaacgaga agacgccgtc cttcaacgtg agcccggcga
    10441 agcagttcta ccactgcttc ggctgcggcg tgcatggcaa cgcgatcggc ttcgtgatgg
    10501 agtattcggg actcacctac cccgatgcca tccgggaact cgcgcagcag gccggcatgg
    10561 aggttcccga ggtccagggg acggtgccgg gccggccggc cgccgcgcaa gccaagggcc
    10621 tcaccgaccg gatgatggaa gcgctcaact attaccgcgc cgaactcaag cgaacgcccg
    10681 aggcgatcga gtacctcaag gggcgcgggg tttccggcga gatcgccgcg cgcttcggcc
    10741 tgggtttcgc gcccgatgcg tggcagtcgc tcaagggcgt gttcgccgac tacgaatccg
    10801 ccgggatgaa ggacaccggc ctggtcatcg attccgaggg tggcgaaggg cacgagggcg
    10861 agccggcgaa gaaggcccgg cgctatgacc gcttccgggg acgcgtcatg ttccccatcc
    10921 tggattcccg cggcaacgtc atcggcttcg gcgggcgcgt tctcggcgac ggcgagccca
    10981 agtacctgaa ttcgcccgag acgccgcttt tcgagaaggg ccgcgagctc tatggcctgt
    11041 ggcaggcgcg gcgggcgatc cgcgacgcga acctcgtcat cgtggtcgag ggctacatgg
    11101 acgtggtggc gctcgcccaa cacggcgtgg agaacgccgt ggccactctc ggaaccgcca
    11161 cgacgccgat gcacgtggcc aagctgctga agcttgccga cagcgtcgtg ttctgcttcg
    11221 acggcgacag cgccgggcgc aaggcggcct ggaaggcgct cgaggtgtcg ctcccggtcc
    11281 tggccgacgg caagtcggtt gcctttctct tcctgcccca ggaggacgat ccggacacct
    11341 tcgtgcgccg gctcggccgg gaggccttcc tcgaggcgct ggccggggcg aagccgctct
    11401 cccagtacct ggtttcggag atcgccgcga aagtcgacat gcgttccgag gaggggcgcg
    11461 cccgcttcct cgcccaggcg aagccgctcg tttcgcaaat cgaagccccg gcgctgggcg
    11521 ccatgatccg caggcgcctg gccgaactgg cggggctcga cccgggcgag ctgcagggct
    11581 acgcgccgca acgggattcg tggcggccgc gccccgcccc ggcggcccgc gtcgtgcgcc
    11641 ggacgcattc cctcgaggcg cagatcctgc agcgggtgct gcgcaagccc gacctcgccc
    11701 ggtcggtcga tcccgacctg gtgtccgacg ggtccgcgga aggccgggcc ctgcacgcgc
    11761 tcctggcctt cgtccgggag ggagaggccg gcctctccct ggggcaggtg atagcccact
    11821 tcgacggctc ggagcacggc ccggcgctcg acgcggcgat cgcgggcggc agcatcctgg
    11881 acgaggtccc ggaggcggag ctcgaccttg cggcggaact cgccgggctg caggacaagc
    11941 tcggcatgca gcgatcggaa cgccgcaagg cggagctcga gatgcgcagc atggctgccg
    12001 gcctcaccgg ggccgaacag gccgaactgg ccggattgaa ggcccggcag gccgcggcca
    12061 agggagtgaa ctccgccctc gaattgccgc ctaaacagtg ataaaataat tggttttctc
    12121 caacgccttg ccccttccgg agatgcgcgt gaagtcctcc accagagatt cgcagaagaa
    12181 cgccaaggac tcgcagaaga acgcgagcaa agaccagcac aaggccgagg tgaagctcgc
    12241 cgaggtcgag gcgcagaaga agcgcctgaa gaacctcatc gtgctgggca aggagcgcgg
    12301 ctacctcacg tacgccgaga tcaacgacca cctgcccgac gagatgaccg atgccgagca
    12361 gatcgagagc gtggtcacga tgctcgcgga catgggcatc gccgtgtacg accaggcgcc
    12421 ggacgccgag acgctcctgc tgaccgaggc ggccaccaat cccgtggccg acgaggacgt
    12481 ggtggaggag gccgaggagg ccctctccct caccgtcgat tccgagttcg gccgcaccac
    12541 ggacccggtg cgcatgtaca tgcgcgagat gggctcggtg gagctgctca cgcgcgaggg
    12601 cgagatcgag atcgccaagc gcatcgagga cggcctcaag cacatgatcc acgcgatctc
    12661 gtcgtgcccg acgacgatcg aggagatcct tcgcctggcc gagctcgtcg agaaggacga
    12721 gatgcgcatc gacgagctgg tcgacgggct ggtggacgcc gacggcgagg acatcgtcgg
    12781 cgaggagatg agcgaggagg aagagctcga ggagatcgag gaggacgagg ccgaggaaga
    12841 cgccgacatg gcgagcgccg acctcgagca gctcaagaac gactccctcg tgcacttcaa
    12901 caagatccgg cgcctctaca agaagatgcg caagatcctc gcggagaagg gctatcgctc
    12961 gcgcgcctac aaggatctcc aggagtcggt ctccggcgag ctcctcatgg tccggttcac
    13021 ggccaagcag gtcgagcacc tgtgcggcgg gcttcgccag ctggtcgagc gcgtgcgcgg
    13081 ccacgagcgc gagatcatgg agctgtgcac gcgcaactcc agcatgccgc ggcagcactt
    13141 catcaaggtg ttccccggca acgagaccaa cctcaagtgg gtcgccgagg agatttcctc
    13201 gggcaaggcc ttcgccaagg cgctcgagcg cttcaagccg gccatcgtgg agcagcagga
    13261 agcgctcatc agcctgcaga aggaagtggg catcccgatc aaggacctga aggagatcgc
    13321 ccg
//
LOCUS       DASUEV010000230         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_742724, whole genome shotgun sequence.
ACCESSION   DASUEV010000230 DASUEV010000000
VERSION     DASUEV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742724"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFV55_07515"
     CDS             complement(<1..655)
                     /locus_tag="VFV55_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX4944187.1"
                     /translation="MDTRTPDLFSPENGDDAAPIERHASQAYLGYAVSTVKSRALPEI
                     ADGLKPVQRRILYAMGDANTQGFAKCARYVGEVLGKYHPHGDSSTYEALVHLAQPFSM
                     RYPLIDGQGNFGSRDGDSAAAYRYTEAKLSRYAELMLAEIGEGTVDFIKNYDGKFDEP
                     VLLPARLPFGLLNGSFGIPVGFSTRIPSHNLKEVAAAAAYVIKHPRAKVEDILEIMPG
                     "
     gene            complement(658..876)
                     /locus_tag="VFV55_07520"
     CDS             complement(658..876)
                     /locus_tag="VFV55_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944188.1"
                     /translation="MAKRQKAFWERAYKGHVLWQGKQKLGKVTLAGAGRYTWEASGRT
                     GASDDLDKAKKAVELAVAVADKQLDLFR"
     gene            complement(972..>2088)
                     /locus_tag="VFV55_07525"
     CDS             complement(972..>2088)
                     /locus_tag="VFV55_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX4944189.1"
                     /translation="RSGGTHEAGFRAGIYDAISTFMDARALAPKGVKLIAEDVWARAC
                     FTLSARIVRTQFHGQTKEKLTTRHAAKLMELCVKDAFELWLNEHPEDGKKLAELAIEQ
                     ALARLAKGKKVERKKSSGIATLPGKLVDCASGDVARNELFLVEGDSAGGSAKEARDKE
                     SQAILPLRGKVLNTMSKDSRTVLANKELQAIATALGVDPHGPEESPDLSGLRYGKVIL
                     MTDADVDGGHIQALLLTFFFMHAPKLVEAGHVYVAQPPLFKVEIPAQGKAKPARRAYC
                     LDDAELEETLATLKKDKVKEGSWEISRFKGLGEMSPEQLWETTMNPDTRRLVRMQLQA
                     KDIAKVKLTFEKLMDAGEAEERRNWMREHWKSVEADV"
BASE COUNT          289 a          704 c          692 g          403 t
ORIGIN      
        1 ggcccggcat gatctccagg atgtcctcga ccttggcgcg cgggtgcttg atgacgtagg
       61 cggccgcggc ggcgacttcc ttcaggttgt gcgagggaat gcgcgtggaa aagcccaccg
      121 ggatgccgaa ggagccgttc agcaggccga agggcaggcg cgcgggcagc agcaccggct
      181 cgtcgaactt gccgtcgtag ttcttgatga aatcgaccgt gccctcgccg atctcggcga
      241 gcatcagctc ggcgtagcgc gagagcttcg cctcggtgta gcggtaggcc gccgccgagt
      301 cgccgtcgcg gctgccgaaa ttgccctggc cgtcgatcag cgggtagcgc atggagaagg
      361 gctgcgcgag gtgcacgagg gcctcgtagg tggaggagtc cccgtgcggg tggtacttgc
      421 cgagcacttc gccgacgtag cgcgcgcact tggcgaagcc ctgcgtgttg gcatcgccca
      481 tcgcgtagag gatgcggcgc tgcaccggct tcaggccgtc ggcgatctcg ggcagcgccc
      541 gcgacttgac ggtggacacc gcgtagccaa gataggcctg cgaggcgtgg cgctcgatcg
      601 gcgcggcgtc gtcgccgttt tcgggcgaga acaggtccgg ggtgcgggta tccatggtca
      661 cctgaacagg tcgagttgtt tgtcagccac cgccaccgcg agctcgaccg ctttcttcgc
      721 cttgtcgagg tcgtcgctcg cgcccgtcct gccggaagcc tcccaggtat agcggcccgc
      781 gccggcgagc gtcaccttgc cgagcttctg ctttccctgc cagagcacgt ggcccttgta
      841 ggcccgctcc cagaacgcct tctgcctctt cgccatggcg ccctccctga aatcggggtc
      901 agagcccgat ttcgtttcgc ttcaaatcgc ttcgggcgat ttgcagcgaa atcgggctct
      961 gaccccgatt ttcatacgtc cgcctccacg ctcttccagt gctcgcgcat ccagttcctt
     1021 ctttcttccg cctcgcctgc gtccatcagt ttctcgaacg tcagctttac cttggcgatg
     1081 tccttggcct gcagctgcat gcgcaccagg cgccgcgtgt ccgggttcat ggtggtctcc
     1141 cagagctgct cggggctcat ctcgcccagg cccttgaagc gcgagatctc ccacgagcct
     1201 tccttcacct tgtccttttt cagcgtggcc agcgtctcct ccagctcggc gtcgtccagg
     1261 cagtaggcgc ggcgcgcggg cttggctttg ccctgcgccg ggatctcgac cttgaacagc
     1321 ggcggctgcg cgacgtagac gtgccccgcc tcgaccagct tgggcgcgtg catgaagaag
     1381 aaagtgagca gcagcgcctg gatgtgcccg ccgtccacgt cggcgtcggt catcaggatc
     1441 accttgccgt agcgcaagcc cgagaggtcg ggcgactcct cggggccgtg cgggtccacg
     1501 ccgagggcgg tggcgatcgc ctgcagctcc ttgttggcga gcacggtgcg cgaatccttg
     1561 gacatggtgt tgagcacttt gccgcgcaac ggcaggatcg cctgcgactc cttgtcgcgc
     1621 gcctcctttg ccgagccgcc ggccgaatcg ccctcgacca ggaacagctc gttgcgcgcc
     1681 acgtcgcccg aggcgcaatc gacgagcttg ccgggcagcg tggcgatgcc ggaggacttc
     1741 ttgcgctcga ccttcttgcc cttggcgagg cgcgcgagcg cctgctcgat ggcgagctcg
     1801 gcgagcttct ttccgtcctc gggatgctcg ttgagccaca gctcgaaggc gtccttgacg
     1861 cacagctcca tcagcttcgc cgcgtggcgc gtggtgagct tttccttggt ctggccgtgg
     1921 aactgcgtgc gcacgatgcg cgcagaaagc gtgaagcagg cgcgcgccca cacgtcctcc
     1981 gcgatcagct tcacgccctt gggcgcgagc gcgcgcgcgt ccatgaaggt gctgatcgcg
     2041 tcgtagatgc cggcgcggaa acccgcctcg tgcgtgccgc ccgagcgc
//
LOCUS       DASUEV010000231         5904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_743269, whole genome shotgun sequence.
ACCESSION   DASUEV010000231 DASUEV010000000
VERSION     DASUEV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743269"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1242
                     /locus_tag="VFV55_07530"
     CDS             82..1242
                     /locus_tag="VFV55_07530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX4944190.1"
                     /translation="MEERLNAPGRVSAQEGGQVGNSREGLLAEVRDGVATVTLDRPAA
                     LNALNLAMLEGLAAWLDGWERDDRVRLVVLRGTGGKAFCAGGDIRELREMFVAGSGVH
                     RHFFEVEYALDHRVHTYPKPVVAVMDGITMGGGMGLAQGATLRIAGPRTKVAMPETRI
                     GFFPDVGATWFLQRSPGRIALYLGLAGIAIGAADSLYAGLVDACLSPDAIVSLETELP
                     RVAAAADPRTALAELGRRLASPPARGELETLRPAIDLHFGRESVAAIFASLAAEQRPE
                     FQPWAAKTLRLLEESSPTLLRVAFQQLRSGRSLGLADCFRLELNLIHGCFAQGDFVEG
                     IRALIVDKDNSPRWNPPSLPEVTDASVAAFFAPRWSPSEHPLRHLRNSRRNA"
     gene            1239..2138
                     /locus_tag="VFV55_07535"
     CDS             1239..2138
                     /locus_tag="VFV55_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4944191.1"
                     /translation="MKLVTFSLTAGSPPRPGLVVDPNRIVDIPAHVAATAGVDFSTML
                     GIIRGGAAARKALDALAARPPATAVAMADVRLHAPIPRPAKNVFCVGWNYLEHFEEGA
                     KKLQDNRELPQWPVFFSKAPTAVIGPTDPVPFDPAISTSLDWEVELGVVIGPGGKNIK
                     AEDAMQHVWGYTVINDVSWRDLQRRHGGQWHKGKSLDGTCPMGPVLVTADSLDHANLR
                     VTCRVNGVTKQDSNTKFLYFKLPRLINDLSMGMTLEAGDIISTGTPEGVGFARTPPEF
                     LKPGDLLETEIEGIGTLRNPIGG"
     gene            2191..3162
                     /locus_tag="VFV55_07540"
     CDS             2191..3162
                     /locus_tag="VFV55_07540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4944192.1"
                     /translation="MPSKIPPGSTVIGRMKIYRRLVAIPTLRQSYARKFAAAAFVSAQ
                     TPLAVFIVYLMVARADWAEMFPLVAALLLASVAGFLGTIWLLREILVPIELTAEALRA
                     YLESRALPDLPVDFPDQAGRLMEGTQYTLTQLNETINRLEHVSDSDELTGIYNRRAGE
                     KRLTEEIARAERDLETFHVAFFALNGFKAVIEAHGHSAGDACFSHVAALLQLNIRRGD
                     WVARWGADELVVGLHRNRSLKQVMERVVAAIAASPCEIAPGQELQLTVSAGVAEYRFG
                     AGKSGLLADTDRALFEARHLAKEKGGSQVCYFHEVATGTPMREVAVA"
     gene            complement(3171..3785)
                     /locus_tag="VFV55_07545"
     CDS             complement(3171..3785)
                     /locus_tag="VFV55_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX4944193.1"
                     /translation="MADVGRAARPRRRGGSPLIEALLVSTGIVALAEIGDKTQLLTLV
                     LAARYKKPWPIVAGIFVATLVNHAIAGAVGAWLTKAVGPEVMRWILGVSFIAMAAWML
                     VPDKLDDDDAAERKVGGVFVTTTLLFFFVEIGDKTQIATVALAARFPSLVAVVMGTTI
                     GMILANAPVAFFGEALARRLPVRTVHVIAALVFAALGVGVLVLG"
     gene            complement(3731..5521)
                     /locus_tag="VFV55_07550"
     CDS             complement(3731..5521)
                     /locus_tag="VFV55_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4944194.1"
                     /translation="MAEYAAPLKDMQFVLKHVVGLDQVNTLPGWEEVTEDVVDAVLEE
                     AAKFAGEVLSPLNASGDKVGAQWKDGKVITAPGFKDAYWQFVNGGWGNIMSPEEFGGQ
                     GLPALLSTPVEEMWGSANIAFKLCPMLTLGAVEALHHNASDELKKRFLPNMISGKWTG
                     TMNLTEPQAGSDLSLVRTKAVPQPDGTFKLTGQKIFITYGDQDYTENIIHAVLARIEG
                     APEGVKGISLFVVPKVLVKPDGSLGDGNGVSCVSIEHKLGIHASPTAVIAYDNAVGYL
                     VGEANRGLEYMFVMMNAARLGVGLEGVSICERAYQRARDWAKDRLQGKPVGLAGGART
                     APIIQHPDVKRMLLTMKANAEATRALAYQVSALLDRARRHPDKDERRRCHALVDLLIP
                     VVKGWSTETGIEVASLGIQVHGGMGFIEETGAAQHLRDARISTIYEGTTGIQANDLVG
                     RKVGREAGATMKLLIGEMRKALAELAAADDAELRIVESRLAKAIDALERATDWIVTFY
                     PADPGAVAAGSVYYLKLSGLAIGGWMMARSAIAATRQLAAGQGDAEFLRGKRLTARFF
                     ADHLLPQVDALAESLMSGGQLVRAAEEALL"
     gene            complement(5532..>5904)
                     /locus_tag="VFV55_07555"
     CDS             complement(5532..>5904)
                     /locus_tag="VFV55_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX4944195.1"
                     /translation="ARPELVAARRVIAGGRGVGSAENFKMIEALADKLGAAIGASRAA
                     VDSGFVPNDYQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIVAINKDPEAPIFQ
                     VADYGLVGDLFQIVPELTAQL"
BASE COUNT          927 a         2087 c         1956 g          934 t
ORIGIN      
        1 acgcgagcgc ttcatggacc tcttcgacca ttccgaccag ctcgagggcg tgaacgcctt
       61 cctcgagaag cgcgcgccgg catggaggaa cgactgaacg cccccggccg ggtgtccgcg
      121 caagaggggg ggcaagtcgg aaattcccgc gaggggcttc tcgccgaggt gcgcgacggg
      181 gtggccaccg tcaccctgga ccggcccgcc gccctcaacg cgctcaacct ggccatgctc
      241 gaggggctgg ccgcctggct cgacggctgg gaaagggacg accgcgtccg gcttgtcgtg
      301 ctgcgtggca cgggcggcaa ggccttctgc gcgggcggcg acattcgcga gcttcgcgag
      361 atgttcgtcg ccggctccgg agtgcaccgg cacttcttcg aagtcgagta cgcactcgat
      421 catcgcgtgc acacctaccc gaagccggtg gtcgcggtga tggacggcat caccatgggc
      481 ggcggcatgg gcctcgcgca aggcgccacc ctgcgcatcg cgggcccgcg cacgaaggtc
      541 gcgatgccgg agacgcgcat cggcttcttc cccgacgtcg gcgcaacctg gttcctgcag
      601 cggtcgcccg gaaggattgc cctgtacctc ggcctcgcgg gcatcgcgat cggcgccgca
      661 gacagcctct acgcggggct cgtggacgcg tgcctgtcgc cggatgcgat cgtctcgctc
      721 gagaccgagc tgccgcgcgt cgctgccgcc gcggatcccc gcaccgctct ggcagagctg
      781 ggccggcgcc tcgccagccc gccggcccgc ggggaactcg aaacacttcg cccggccatc
      841 gacctgcact tcggccgcga aagcgtggct gccatcttcg cctccctggc ggccgagcag
      901 cggcccgagt tccagccctg ggccgcgaaa acgctccggc tcctcgagga gagctcgccg
      961 acattgctcc gtgtcgcctt ccagcaactg cggtccggcc gaagcctcgg gctcgcggac
     1021 tgcttccggc tggaactcaa cctcatccac ggctgcttcg cgcagggcga tttcgtggag
     1081 ggcatccgcg ccttgatcgt ggacaaggac aattcccccc gctggaatcc accctccctt
     1141 cccgaggtca ccgacgcgtc ggtcgcggcg ttcttcgccc cgcgctggtc cccgtccgaa
     1201 cacccccttc gccatcttcg aaactccagg aggaatgcat gaaacttgtc accttctcgc
     1261 tgaccgcagg cagcccgccg cgccctggcc tggtcgtcga ccccaaccgc atcgtcgaca
     1321 ttccggccca cgtggcagcg acggccggcg tggacttctc cacgatgctc ggcatcatcc
     1381 ggggaggggc cgccgcgaga aaggcgctgg acgcgcttgc agccaggccc ccggcgaccg
     1441 ccgtggcgat ggccgacgta aggctgcacg cgcccatccc gcgcccggcc aagaacgtgt
     1501 tctgcgtggg ctggaactat ctcgagcact tcgaggaagg cgcgaagaag ctgcaggaca
     1561 accgcgaact gccgcagtgg ccggtcttct tcagcaaggc gccgaccgcc gtcatcggcc
     1621 ccaccgaccc ggtgcccttc gatcccgcga tctccacttc gctcgactgg gaagtggagc
     1681 tcggcgtggt gatcggcccc ggcggcaaga acatcaaggc cgaggatgcg atgcagcacg
     1741 tgtggggcta caccgtcatc aacgacgtct cctggcgcga cctgcagcgc cgccacggcg
     1801 gccagtggca caagggaaag agcctcgacg gcacgtgccc catgggcccg gtgctggtga
     1861 cggccgacag cctcgaccac gccaacctgc gcgtgacctg ccgcgtgaac ggggtcacga
     1921 aacaggactc gaacacgaag ttcctctatt tcaagctgcc gcgcctcatc aacgacctgt
     1981 ccatgggcat gacgctcgag gccggcgaca tcatctcgac aggtaccccc gagggtgtgg
     2041 gctttgcccg gactcccccg gaattcctca agcccggcga cctgctcgaa accgagatcg
     2101 agggcatcgg cacgctgcgc aacccgatcg ggggttgacg ccgcccgggg gaacacggcc
     2161 agaatgggcc cgttcccctg gagcacaccc atgccgagca agatcccgcc cggatccacc
     2221 gtgatcgggc gcatgaaaat ctaccgacgc ctcgtcgcga tcccgacgct gcgccagagc
     2281 tacgcgcgca agttcgcggc cgcggcgttc gtcagcgcgc aaacaccgct cgcggtgttc
     2341 atcgtctatt tgatggtcgc ccgtgcggac tgggcggaaa tgttccccct ggtcgccgcg
     2401 cttctcctgg catcggtggc cggcttcctc gggacgattt ggctccttcg cgagatcctg
     2461 gttcccatcg agctcaccgc ggaagcgttg cgcgcctacc tcgaaagccg cgcgctgccc
     2521 gacctgccgg tcgacttccc ggaccaggcc gggcgcctca tggagggcac gcagtacacc
     2581 ctgacgcagt tgaacgagac gatcaaccgc ctggaacacg tctcggattc cgacgagctc
     2641 accggtatct acaaccggcg cgccggcgag aagcgcctga cggaggagat cgcgcgggcc
     2701 gagcgcgacc tcgagacatt ccacgtcgcc ttcttcgccc tcaacggatt caaggcggtc
     2761 atcgaggcgc atggccacag cgctggggac gcctgcttct cgcacgtcgc ggcgctgctg
     2821 cagctcaaca tccgccgcgg cgactgggtg gcgcgctggg gcgccgacga gctggtcgtc
     2881 ggcctgcacc gcaaccgctc cctcaagcag gtgatggaaa gggtcgtggc ggccatcgca
     2941 gccagcccct gcgagatcgc gcccgggcag gaactgcagc tcacggtgag cgccggcgtc
     3001 gcggagtacc gcttcggcgc cggcaagtcc gggcttctgg cggacaccga tcgcgccctg
     3061 ttcgaggcca ggcacctggc aaaggaaaag ggcggctcgc aggtgtgcta cttccacgag
     3121 gtcgccaccg gcacgccgat gcgcgaggtg gccgtggcct gagcctcgcc tcagcccagc
     3181 accagcactc ccacgcccag ggcagcgaat accagcgccg cgatcacgtg caccgtgcgc
     3241 acgggcaggc gcctggcgag cgcctcgccg aagaaggcca ccggcgcgtt ggcgaggatc
     3301 atcccgatgg tggtgcccat gacgaccgcc acgagggacg gaaagcgcgc cgcgagcgcc
     3361 accgtcgcga tctgcgtctt gtcgccgatc tccacgaaga agaagagcaa cgtcgtggtg
     3421 acgaacacgc cgcccacctt gcgctcggcc gcgtcgtcgt cgtcgagctt gtccgggacc
     3481 agcatccacg cggccatcgc gatgaacgag acgccgagga tccagcgcat cacctccggg
     3541 ccgacggcct tcgtgagcca cgcgcccacg gcgccggcga tcgcgtggtt cacgagcgtc
     3601 gcgacgaaga tgccggcgac gatgggccag ggcttcttgt agcgcgccgc aagtacgaga
     3661 gtgagaagct gggtcttgtc cccgatttcg gcgagcgcga cgatgccggt cgagacgagc
     3721 agggcctcga tcagaggaga gcctcctcgg cggcgcggac gagctgcccg cccgacatca
     3781 gcgattcggc cagcgcgtcg acctgcggca ggaggtggtc ggcaaagaag cgcgccgtga
     3841 gacgctttcc gcgcaggaat tccgcgtcac cctgccctgc ggcgagttgc ctggtggcgg
     3901 cgatggcgga cctcgccatc atccagccgc cgatggcgag cccggagagc ttgaggtagt
     3961 agaccgaacc ggccgcgacc gcgcccggat cggccgggta gaaggtcacg atccagtcgg
     4021 tggcccgctc gagcgcgtcg atggcctttg cgagacggct ttcgacgatg cgcaactcgg
     4081 cgtcgtccgc tgcggcgagc tctgccaggg ccttgcgcat ttcgccgatg agcaacttca
     4141 tcgttgcgcc cgcctcgcgc ccgaccttgc ggccgaccag gtcgttggcc tggatgcccg
     4201 tggtgccttc gtagatcgtg gagatgcgcg cgtcgcgcag gtgctgcgcg gcgcccgttt
     4261 cctcgatgaa gcccatgccg ccgtgcacct ggatgccgag ggaagcgact tcgatgccgg
     4321 tttcggtgga ccagcccttc accaccggga taaggaggtc gacgagcgcg tggcaacggc
     4381 gccgctcgtc cttgtccgga tgcctgcgtg cgcggtcgag aagtgccgag acctggtagg
     4441 ccagcgcgcg cgtggcctcg gcgttcgcct tcatcgtgag gagcatgcgc ttcacgtcgg
     4501 ggtgctggat gatcggcgcc gtccgggcgc cgcccgccag gcccaccggc ttgccctgca
     4561 ggcgatcctt cgcccagtcg cgcgcgcgct ggtaggcgcg ctcgcagatc gacacgccct
     4621 cgaggcccac gccgaggcgc gccgcgttca tcatgacgaa catgtactcg aggccgcgat
     4681 tggcctcgcc cacgaggtag cccacggcgt tgtcgtaggc gatcacggcc gtcggactcg
     4741 cgtggatgcc caacttgtgc tcgatggaaa cgcaggacac gccgttcccg tcgcccaggg
     4801 atccgtccgg cttgaccagg actttcggca ccacgaagag cgagatgccc ttcaccccct
     4861 cgggcgcgcc ctcgatgcgg gcgagcacgg cgtggatgat gttctcggtg tagtcctggt
     4921 cgccgtaggt gatgaagatc ttctgcccgg tgagcttgaa ggtcccgtcg ggctgcggca
     4981 cggccttggt gcgcacgagc gagaggtcgc tgcccgcctg cggctcggtg aggttcatgg
     5041 tgccggtcca cttgcccgag atcatgttgg gcaggaagcg cttcttcaat tcatcggagg
     5101 cattgtggtg cagcgcctcc acggcgccca gcgtgagcat cgggcagagc ttgaaggcga
     5161 tgttggccga gccccacatt tcctccaccg gcgtggagag cagcgccggc agcccctgcc
     5221 cgccgaactc ctcgggggac atgatgtttc cccagcctcc gttcacgaac tgccagtagg
     5281 catccttgaa gccgggggcg gtgatgacct tgccgtcctt ccactgcgcg cccaccttgt
     5341 cgccgctcgc gttcaggggc gagagcacct cgccggcgaa tttcgccgct tcctccagga
     5401 ccgcatcgac cacgtcctcg gtcacttcct cccagcccgg cagggtgttc acctggtcga
     5461 ggccgaccac gtgcttcagc acgaactgca tgtccttcag gggggcggcg tattcggcca
     5521 cggaattctc ctcaaagctg cgcggtgagt tcgggaacga tctggaagag gtcgcccacc
     5581 agcccgtagt ccgccacctg gaagatgggt gcctccgggt ccttgttgat cgccacgatc
     5641 accttgctgt ccttcatgcc ggcgaggtgc tggatggcgc ccgagatgcc caccgcgatg
     5701 tagagctcgg gggcgacgat cttgcccgtc tggcccacct ggtagtcgtt gggaacgaag
     5761 cccgagtcga ccgccgcgcg cgaggcgccg atggccgcgc ccagcttgtc ggccagcgcc
     5821 tcgatcatct tgaagttctc ggcgctgccc acgccccggc cgccggcgat cactcgtcgc
     5881 gccgccacca gctcgggacg cgcc
//
LOCUS       DASUEV010000232         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_872654, whole genome shotgun sequence.
ACCESSION   DASUEV010000232 DASUEV010000000
VERSION     DASUEV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872654"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFV55_07560"
     CDS             <1..683
                     /locus_tag="VFV55_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4944196.1"
                     /translation="LRKGKRTGEFPNIIVMAGDGGTTDIGLQAMSAMMYRGHAALFIC
                     YDNEAYTNTGIQTSPTTPYGANTTFTPPGPAIPEGKRLFPKDPPQLVIGGHPAVKYVA
                     TASVAYPVDLINKVRKALNVVGPTFLHIHCPCPKGWGFPAEKTVEVARMAIETGMWNM
                     YEWENGVYRYQFKPKKYKPVKEYLKLQQRFAHLQEEHIAKLQAFVDAKVKAPGIAVET
                     PVPTPEEA"
     gene            774..1730
                     /locus_tag="VFV55_07565"
     CDS             774..1730
                     /locus_tag="VFV55_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX4944197.1"
                     /translation="MNGSLPTRVRFNRIRFESIGGLGADSAAHALAATTVEKKGLDAA
                     HLPSGGLERKGSRVRSEVLLASSDRPIRDGASGGAPDAIVVFHAALLRDPRTFAGLRK
                     DGTFIYNAPAKSIPEELAALPRTARVLRIDAAGIAAKEKCAMNAVLLGALCGALPYVD
                     AEAVLEGFCADEPGRNGFRRGMKEPETLADVGEADGDLSLSLGSRPGTGSAGGILSDP
                     GTTVWNDVSAARTGFIPAFNRERCIHCGLCDLFCPDSCLVWEKGEEGGRFERELSGVD
                     YRYCKGCLRCVESCPASAMLKKAETPAFVEQRSVPMFPEIVE"
     gene            1727..3889
                     /locus_tag="VFV55_07570"
     CDS             1727..3889
                     /locus_tag="VFV55_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944198.1"
                     /translation="MNKPPGTMARLAQSPARQRIEFRSGDEAASLAARAVGFEAAGLY
                     PAPPSNQVALCYGAALGGVRVLGATNSRGLVAALEQLAVQSAARVPMVLNVAARALAA
                     MPGSGCDHSALYQALDAGWIVLCARDPQAVYDLNLAAVRIGEHAEVRLPVLVAYDGHV
                     TSDEKRRISVLDEPEALERFLGTRAQAPTPLDVAHPATYGAPMDEDDFLDNKAQLSQA
                     MDAARRVIPEVFAELGALTGRTYPVLEAYAMDDAETAVVLLNSAAETAKEAADVWRDD
                     ARKVGVLSPNVLRPFPAEEFRALLRHVNAVTVGDRADSYGAGGGSLSMQVRAALQQDV
                     DNSTIVLARIYGLGGAPFHATDAEQFLDEAFAAATAGEAREPFAYHGATHGEAAPKAP
                     KGLPPIRTGEVTRGMAKVNDATGRLEVELAPLWKMTSVPSRIAPGHAACPGCGAFTSL
                     HQLYKVLEGDLVVLFQTGCAIVVSSSHGATAHRVNCIHNAAGNGAATLSGLVEAYQQR
                     VRRGELPPSGDVTFVLVTGDGGMEADLADVIGAAHRTPRMMILEYDNQGRMSTGGQAT
                     HSTTLGARHGNTAQLLAACNLPYVFTASEGFAEDLMRKAAKAQWYARNEGMAYGKILS
                     FCPLNWKTADDAAQPALQAAIDSCFFPLYEVEHGHTAITYDPDAMGRRKPVAEWLKLM
                     GKTAPLLDLVNEAIVEEIQAETERRWARLKAMHEHPLL"
     gene            3904..>4121
                     /locus_tag="VFV55_07575"
     CDS             3904..>4121
                     /locus_tag="VFV55_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEX4944199.1"
                     /translation="MTRILETRRLTPVTKLFRLDAPAIAASARPGQFVMLRVREGGER
                     IPITIADYDRAAGTLTLVVQEVGATTHRV"
BASE COUNT          692 a         1464 c         1389 g          576 t
ORIGIN      
        1 tgctgcggaa gggcaagcgc acgggggagt ttcccaacat catcgtcatg gcgggcgatg
       61 gcggcaccac ggacatcggg ctgcaggcga tgtcggcgat gatgtaccgc ggccacgccg
      121 cgctcttcat ctgctacgac aacgaggcgt acaccaacac cggcatccag acctcgccga
      181 cgacgcccta cggcgccaac acgacgttca cgccgcccgg ccccgcgatt cccgagggca
      241 agcggctctt cccgaaggat ccgccgcagc tcgtcatcgg cggccacccc gcggtcaagt
      301 acgtggcaac cgcctccgtg gcctacccgg tggacctcat caacaaggtc cgcaaggcgc
      361 tgaacgtggt cgggccgacg ttcctgcaca tccactgccc gtgcccgaaa ggctggggct
      421 tccccgccga gaagaccgtg gaagtcgccc gcatggcgat cgagaccggc atgtggaaca
      481 tgtacgagtg ggaaaacggc gtgtaccggt accagttcaa gccgaagaag tacaagccgg
      541 tgaaggagta cctcaagctg cagcagcgct tcgcgcacct gcaggaagag cacatcgcga
      601 agctccaggc gttcgtcgac gcgaaggtga aggcgcccgg catcgcggtg gaaacgccgg
      661 tcccgacacc cgaagaagct tgaagctctc cctccccctc gatgcagggc caggggtctc
      721 ccctctcccc ccggagaggg gccggaggta aaggaaacgc cggggagaag gtcatgaacg
      781 gatcgctgcc gactcgagtc cggttcaaca ggatccggtt cgagtccatc ggagggctgg
      841 gagccgattc ggcagcgcac gccctcgcag cgaccacggt cgagaagaag gggttggacg
      901 cggcacacct gccctccggc ggcctcgagc gcaagggttc gcgggtgcgc tccgaggtct
      961 tgcttgcctc gtccgaccgg ccgatccgcg atggcgcaag cggcggggca cccgatgcga
     1021 tcgtcgtctt ccatgccgcc ctgctgcgcg atccccggac cttcgcgggg ctgcgcaagg
     1081 acggcacgtt catctacaac gcgccggcga aatcgattcc ggaggaactc gccgcgctgc
     1141 cgcgcacggc acgcgtcctg cgcatcgatg ccgccggtat cgccgccaaa gagaagtgcg
     1201 caatgaacgc ggtgcttctc ggagcccttt gcggcgcctt gccatatgtc gatgccgaag
     1261 ccgtgctcga aggcttctgc gcggacgagc ccggcaggaa tggattccgt cgcgggatga
     1321 aggagccgga gacgctggcc gacgtcggcg aggccgacgg agatctctcc ctcagcctcg
     1381 gaagcaggcc cgggacaggg tcggcgggcg gcatcctttc cgatcccggc accacggtct
     1441 ggaatgatgt atccgccgca cgcaccggtt tcatccccgc cttcaatcgc gagcgttgca
     1501 tccactgcgg cctgtgcgac ctcttctgcc ccgactcctg cctggtgtgg gagaagggcg
     1561 aggaaggcgg ccgcttcgaa cgcgagctct ccggcgtgga ctaccgctac tgcaaggggt
     1621 gcctgcgctg cgtcgagtcc tgcccggcga gcgcgatgct gaagaaggcc gagacgccgg
     1681 ccttcgtgga gcagcgttcc gtgccgatgt tcccggagat cgtcgaatga acaagccgcc
     1741 cggcacgatg gcccgccttg cgcaatcgcc cgcgcggcag cgcatcgaat tccgcagcgg
     1801 cgacgaggcc gcgtcgctgg cggcacgcgc ggtgggattc gaggccgccg gactctaccc
     1861 cgccccgccc tcgaaccagg tcgccctgtg ctacggcgcc gcgctcggcg gagtgcgggt
     1921 gctgggcgcc acgaactctc ggggcctcgt cgccgcgctc gagcagcttg ccgtgcaatc
     1981 ggccgcgcgc gttcccatgg tgctgaacgt cgccgcccgc gccctggcgg caatgcccgg
     2041 cagcggctgc gatcattcgg ccctctacca ggcgctcgat gccggctgga tcgtgctctg
     2101 cgcgcgcgat ccacaggcgg tgtacgacct caacctcgcc gcggtcagga tcggcgagca
     2161 tgccgaagtg cgcctcccgg tcctcgtggc gtacgacggc cacgtcacca gcgacgagaa
     2221 gcgccgcatc agcgtgctcg acgagcccga agcgctcgag cgcttcctcg gcacgcgagc
     2281 gcaggcgccg acgccgctcg acgtggcgca cccggccacc tacggcgcgc cgatggacga
     2341 ggacgacttc ctcgacaaca aggcgcagct ctcgcaggcg atggacgccg cgcgacgcgt
     2401 gattcccgag gtcttcgcgg agctgggggc gctcaccggc cgcacctatc ccgtgctcga
     2461 agcctacgcg atggacgacg cggagaccgc cgtggtgctg ctcaattccg cggccgaaac
     2521 ggcgaaggag gccgctgacg tctggcgcga cgacgcgcgc aaggtgggcg tgctgtcgcc
     2581 caatgtgctg cgcccctttc ccgccgagga attccgcgcg ctgctgcgcc acgtgaacgc
     2641 cgtgaccgtc ggcgaccgcg ccgactcgta cggtgcgggc ggcggcagcc tctcgatgca
     2701 ggtgcgcgcc gcgctccagc aggacgtcga caactccacg atcgtccttg cccgcatcta
     2761 tggcctgggc ggagcgccgt tccacgcgac cgatgccgag caattcctcg acgaggcctt
     2821 cgcggcggcg acagcgggcg aggcgcgcga gcccttcgcc taccacggcg caacgcacgg
     2881 cgaggccgcc ccgaaggcac cgaagggcct gccgccgatc cgcaccgggg aagtcacccg
     2941 cggcatggcg aaggtcaacg acgccaccgg ccgcctcgag gtcgaactgg ccccgctgtg
     3001 gaagatgact tcggttccgt cccggatcgc cccgggccac gcggcctgcc cgggctgcgg
     3061 cgcattcacc tcgctgcacc agctctacaa agtgctcgaa ggcgacctgg tggtgctgtt
     3121 ccagaccggc tgcgcgatcg tcgtatcgag cagccacggc gcgacggcgc accgtgtcaa
     3181 ctgcatccat aacgcggcgg gaaacggcgc ggcgacgctc tccggcctcg tcgaggcgta
     3241 tcaacagcgc gtgcgccgcg gcgaactgcc gccgtccggg gacgtcacct tcgtgctggt
     3301 taccggcgac ggcggcatgg aagcggatct cgccgacgtg atcggcgcgg cccaccgcac
     3361 cccgcgcatg atgatcctgg agtacgacaa ccagggccgc atgagcaccg gcgggcaggc
     3421 cacccattcg acgacgctcg gagcgcgcca cggcaacacc gcccagctcc tcgcggcctg
     3481 caacctgccg tacgtgttca ccgcgagcga gggcttcgcc gaggacctca tgcgcaaggc
     3541 ggcaaaggcg cagtggtacg cgaggaacga ggggatggcc tacgggaaga tcctctcctt
     3601 ctgcccgctc aactggaaga ccgcggacga tgccgcgcag ccggccctgc aggcggcgat
     3661 cgacagctgc ttcttcccgc tgtacgaggt cgagcacggt catacggcca tcacctacga
     3721 cccggacgcg atggggcgcc gcaagcccgt ggccgagtgg ctgaagctca tgggcaagac
     3781 ggcgccgctc ctggacctgg tgaacgaggc gatcgtcgag gagatccagg ccgagaccga
     3841 gcgccgctgg gcgcggctca aggccatgca cgagcacccg ctgctctagg ccggggaatc
     3901 ccgatgacac gcatcctcga aacgcgcagg ctcaccccgg tcaccaagct cttccggctc
     3961 gacgcgccgg ccatcgcggc gtcggcgcgc cccgggcaat tcgtgatgct gcgcgtgcgc
     4021 gaaggcggcg aacgcatccc gatcaccatc gccgactacg atcgcgccgc gggcacgctc
     4081 accctcgtcg tgcaggaagt cggcgcaacg acgcatcgcg t
//
LOCUS       DASUEV010000233         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_396361, whole genome shotgun sequence.
ACCESSION   DASUEV010000233 DASUEV010000000
VERSION     DASUEV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396361"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFV55_07580"
     CDS             <1..928
                     /locus_tag="VFV55_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX4944200.1"
                     /translation="DIFTMAEKEVGVPYGSIKATVLIETVVAAFEMDEILWELRDHSA
                     GLNIGRWDYIFSCIKKFRSHPDFCLADRAQVTMTSPFMRAYALLLVKTCHRRNAPAMG
                     GMAAQIPIKNDPAANEAAMEKVRADKLREVTDGCDGTWVAHPGLVPIAREIFDKHMPQ
                     PNQYGKQHPEVNVTAKDLLDFKPEAPITEAGLRNNVQVGIQYIGSWLGGNGCVPIFNL
                     MEDAATAEISRSQVWQWMRSPKGVLADGRKVTRQLFHEILADELKKTPSIVGAEAYAA
                     GRYAEGARLFEEITASDEYVEFLTLPAYRALA"
     gene            1089..1292
                     /locus_tag="VFV55_07585"
     CDS             1089..1292
                     /locus_tag="VFV55_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944201.1"
                     /translation="MKPGETTDFFFAGACSMPGLLFWLGDPGLAVIVLVGLLIAACLG
                     AMADPEVPFRSMPEPDQDDRAGR"
     gene            complement(1294..1716)
                     /locus_tag="VFV55_07590"
     CDS             complement(1294..1716)
                     /locus_tag="VFV55_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944202.1"
                     /translation="MDIASRIGRMGFRKWYERQLIDSHLSFTTCFLSGITVAACLEEL
                     SFAEFGWKPATLLATVFVATALGWYSWRRYITVLERAEIYGSHSSCPACKTYARFDIL
                     ATGHVAEGPYVDPQPAVLPYPWIRVRCRKCGTTWRMPD"
     gene            complement(1757..2710)
                     /gene="lipA"
                     /locus_tag="VFV55_07595"
     CDS             complement(1757..2710)
                     /gene="lipA"
                     /locus_tag="VFV55_07595"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX4944203.1"
                     /translation="MADTRTPEGRLAAKRAGKTARIPIKVVSAEPLRKPEWIRVRAGG
                     GGRFHEIKKILRDAKLNTVCEEASCPNIGECFGHGTATFMIMGDLCTRRCPFCDVAHG
                     RPQPLDADEPRHLGETIAAMRLRYVVITSVDRDDLRDGGAQHFVDCIRAVRELSPGTK
                     IEILTPDFRGRLDRALTILADEPPDVMNHNLETVPRLYAQARPGADYAHSLRLLKDFK
                     AARPGTPTKSGLMVGLGETSDEILQVMRDLRDHDVDMITIGQYLQPSKSHLPVLRFAH
                     PDEFRMFEEEAATMGFANAACGPMVRSSYHADRQARESGVV"
     gene            complement(2719..>3241)
                     /gene="lipB"
                     /locus_tag="VFV55_07600"
     CDS             complement(2719..>3241)
                     /gene="lipB"
                     /locus_tag="VFV55_07600"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX4944204.1"
                     /translation="STPDELWFVEHPPVFTLGLAAKPEHVLDAGAIPVVRIDRGGQVT
                     WHGPGQLVAYVLLDLKRAGYGVKELVRRLEQSVIDLLAGYGVTGARQAGMPGVYVHGA
                     KIAAVGLRVSRGCSYHGISLNVDADLAAFSRINPCGYPGLAAARLSDLGVRDTMAAVQ
                     QRWQAHLATALFP"
BASE COUNT          520 a         1062 c         1145 g          514 t
ORIGIN      
        1 cgacatcttc acgatggccg agaaggaagt cggcgtcccg tacggctcca tcaaggccac
       61 ggtcctcatc gagacggtcg tcgccgcctt cgagatggac gagatcctgt gggagcttcg
      121 cgaccactcc gccggcctca acatcggccg ttgggactac atcttctcgt gcatcaagaa
      181 gttccgctcc cacccggact tctgcctggc cgaccgggcg caggtcacga tgacctcccc
      241 gttcatgcgc gcctatgcgc ttctgctggt taagacctgc caccgccgca atgcaccggc
      301 gatgggaggc atggccgcgc agatccccat caagaacgac ccggccgcca acgaggccgc
      361 gatggaaaag gtgcgcgccg acaagcttcg cgaggtgacc gacggctgcg acggaacgtg
      421 ggtcgcgcac ccgggcctgg tgccgatcgc cagggagatc ttcgacaagc acatgccgca
      481 gcccaaccag tacggaaagc agcatcccga ggtgaacgtg acggcgaagg accttctcga
      541 tttcaagccg gaggcgccga tcaccgaggc agggctgcgc aacaacgtcc aggtgggcat
      601 ccagtacatc ggttcctggc tgggcggcaa cggctgcgtg cccatcttca acctgatgga
      661 ggacgcggcc accgcggaga tttcgcgctc ccaggtgtgg cagtggatgc gctcgcccaa
      721 gggcgtgctg gccgacggac gcaaggtgac ccggcaactc ttccacgaga tcctggccga
      781 tgaactgaag aagacaccct ccatcgtcgg cgcggaggct tacgccgccg gcaggtacgc
      841 cgagggcgcc aggcttttcg aggagatcac cgcgtccgac gagtacgtcg agttcctgac
      901 gctgcccgcc taccgcgcgc tcgcctgatc ttcgcggcgg cgcccggccg ccggtgcacc
      961 ccgaaggccc gccgtactcc ggcgggcctt gtgtttcgcg gggtacggaa tatcctgcac
     1021 tatcgccgcg gcccctgaag aatcgggcgc ggacgcattg gcgtcatccc tctgggaagg
     1081 aggcgacgat gaagccgggc gaaacgacgg atttcttctt tgcgggagcg tgctcgatgc
     1141 ccggcctgct gttctggctg ggcgatccgg ggctcgcggt gatcgtgctg gtcggactgc
     1201 tcatcgcggc ctgcctcggc gcgatggccg accccgaggt gccgttccgg tcgatgccgg
     1261 agccggacca ggacgatcgc gccgggcgct aggctagtcc ggcatgcgcc atgtcgtgcc
     1321 gcacttgcgg caacgcacgc gaatccacgg gtagggcaac acggctggct gcgggtcgac
     1381 atagggacct tcggcgacgt gccccgtggc gaggatgtcg aagcgcgcgt aggtcttgca
     1441 cgcggggcag ctcgagtggc tgccgtagat ttcggcgcgc tcgagcacgg tgatgtagcg
     1501 ccgccacgag taccagccga gcgccgtcgc gacaaagacg gtcgcgagga gggtcgccgg
     1561 cttccagccg aactccgcga agctcagttc ctcgaggcag gcagcgaccg tgatcccgga
     1621 aaggaagcag gtggtgaacg agaggtggct gtcgatgagt tgccgctcgt accacttgcg
     1681 aaagcccatc cggccgatgc gcgacgcgat gtccatttcg cgacaatacg cgcgcaggcc
     1741 gggctggtgc gcggcttcac acgacccccg actcccgggc ctgccggtcc gcgtggtagc
     1801 tggaacgcac catcggcccg caggcggcgt tggcgaagcc catcgtggcg gcttcctcct
     1861 cgaacatgcg gaactcgtcc gggtgcgcga agcgcaagac gggaaggtgg cttttcgagg
     1921 gctgcaggta ctgcccgatg gtgatcatgt ccacgtcgtg gtcgcgcagg tcgcgcatca
     1981 cctgcaggat ttcgtcgctg gtctcgccca gcccgaccat gagacccgac ttggtcggcg
     2041 tgccggggcg cgcggccttg aagtccttca ggagcctgag cgaatgcgcg taatccgccc
     2101 cgggccgggc ctgcgcgtaa aggcgcggca ccgtctcgag gttgtggttc atcacgtcgg
     2161 gcggttcgtc ggcgaggatc gtgagcgcgc ggtcgaggcg tccgcggaaa tccggcgtga
     2221 ggatctcgat cttcgtgccc ggcgacagct cgcggaccgc gcggatgcag tcgacgaagt
     2281 gctgcgcgcc gccgtcgcgc aggtcgtcgc ggtccacgct cgtgatcacc acgtagcgaa
     2341 gccgcatcgc ggcgatggtc tcgccgaggt gacgcggctc gtcggcgtcc aggggttgcg
     2401 ggcggccgtg ggcgacgtcg cagaacgggc agcgccgcgt gcacaggtcg cccatgatca
     2461 tgaaggtggc ggtgccgtgg ccgaagcact cgccgatgtt ggggcacgag gcttcctcgc
     2521 agacggtgtt gagcttcgcg tcgcgcagga tcttcttgat ctcgtggaag cgcccgccgc
     2581 cgccggcgcg tacgcggatc cattcgggct tgcgcagcgg ctcggcggaa accaccttga
     2641 tggggatgcg cgccgtcttg ccggcgcgct tggcggcgag cctgccttcg ggtgtccggg
     2701 tatcggccat ggcgggattc agggaaagag cgcggtggcg aggtgcgcct gccagcgctg
     2761 ctgcacggcg gccatcgtat cacgcacccc gaggtcgctc aggcgcgcgg ccgcgagtcc
     2821 ggggtagccg caggggttga tgcgcgagaa tgccgccaga tccgcgtcca cgttgagcga
     2881 gatgccgtgg tagctgcagc cgcgcgagac gcgaagcccc acggcggcga tcttggcgcc
     2941 gtgcacgtag acgcccggca ttcccgcctg ccgggcaccc gtgaccccat agccggcgag
     3001 gaggtcgatc accgattgct cgaggcggcg caccagttcc ttcaccccgt agccggcgcg
     3061 cttcaggtcg agaaggacat aggcgacgag ctgccccggc ccatgccagg tgacctggcc
     3121 gccacggtcg atgcgcacga ccgggatggc tcccgcgtcc agcacgtgtt cgggcttcgc
     3181 cgcgaggccg agggtgaaaa cgggcgggtg ctcgacgaac cacagttcgt cgggcgtgga
     3241 a
//
LOCUS       DASUEV010000234         8503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_76218, whole genome shotgun sequence.
ACCESSION   DASUEV010000234 DASUEV010000000
VERSION     DASUEV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8503
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76218"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFV55_07605"
     CDS             <1..203
                     /locus_tag="VFV55_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944205.1"
                     /translation="GTLIGTAVVDNLGNYLFDQVLTSTVGVQNPTNSLGNGTGFWCSP
                     PKTMRITSSLSTATASPPIQIK"
     gene            complement(330..812)
                     /gene="moaC"
                     /locus_tag="VFV55_07610"
     CDS             complement(330..812)
                     /gene="moaC"
                     /locus_tag="VFV55_07610"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX4944206.1"
                     /translation="MNEKLTHFDEGGRAWMVDVGGKDETARRAVASGVIRMRPETLAL
                     IRSGTAKKGDVLGVARIAAIQAAKRTWELIPLCHPIALTALEVSFDVDEGSSLVACRA
                     VAECRGRTGVEMEALAAVSAGLLTIYDMCKAVDRGMTMGDIRLLEKEGGKSGHWVGRE
                     "
     gene            904..2364
                     /locus_tag="VFV55_07615"
     CDS             904..2364
                     /locus_tag="VFV55_07615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4944207.1"
                     /translation="MLRRLVALALCAALLPAQAQGLPDLGDSSDAVLSDNQERTIGNR
                     VMREVRIDPAFVDDPEISDYLKSLGNRLLGAADPPRRQIEFFVVQDDTINAFALIGGH
                     IGVHTGLILLTRNEAELSGVVAHEIAHILQRHQSRLYQGQGKYQLASLAALALAILAS
                     RGSSSQSGQVTEAAMVGASAIMMQGQLDYTREHEREADRVGVTLLERAGYDPRGMVSF
                     FERMQRANRLSEFKGAPSYLRTHPLTVERIADMQSRVEAMPARLVPDSFEYRIARAKI
                     RAGSGSATEAVTYFRNQLADRSVLRPREDVYGLALALRRTRDFVEAEKELATIRGSEG
                     SHPAFEWLAAQLQADQGRMDAALATCRAALKSHPQYRGLVYGLAQLLLDAGRKAEAIA
                     WLQEKVRASPEDARLYELQSRAYAASGNRLAQHRAQAEAYFQRGNLAAAVDQLELATK
                     VRDSDFYELSMAEARLRELRSQLANEREAEKALKLS"
     gene            2364..2708
                     /locus_tag="VFV55_07620"
     CDS             2364..2708
                     /locus_tag="VFV55_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4944208.1"
                     /translation="MSRRVRALAAAAALAAGAAWGQATEGRDLFARPDKGNCIACHQV
                     PDGWGAAVRADVGARLDAARLKGWDRARLRDLLDDPMRANPDTVMPPYGRHRILDARE
                     IESLIDYLHALP"
     gene            2695..3549
                     /gene="soxA"
                     /locus_tag="VFV55_07625"
     CDS             2695..3549
                     /gene="soxA"
                     /locus_tag="VFV55_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX4944209.1"
                     /translation="MRFPERRLRIALLFAVALPLHAWAGAESVRTDIVNRIRSQYPAL
                     APADLGAGSAAFDSARLAEAERNRPAPGAAEAVDAGRRIWTRKFAKGRSLAGCFPNGG
                     RRVAAAYPQYDQRVKGLVTLETAINQCLKTHGQPLLEPGDPDTMGVVLAYLRTLSTGQ
                     KIAVRVASAQAEARFEEGRRLYFTRMGQQNYSCASCHVNHAGKFFGDAAIPAPVGAAA
                     QWPYLRDGRPVTLQMRIRECLDRMGAAPFPAGSDELAHLDYFLSYLSNGLVVRPNEWR
                     PHLVHGNL"
     gene            3623..4849
                     /locus_tag="VFV55_07630"
     CDS             3623..4849
                     /locus_tag="VFV55_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4944210.1"
                     /translation="MTSKLLRPSVLVAILVLAAAGGYAWWQYGSGTTPAERWRLEAVD
                     KGDVVQVISANGNVNPVTSVSVGTQVSGTLIKLHADYNSVVKEGQLLAELDPALFNAA
                     LAQSEANLNSARASLRLAETKEARTRSLVAKGFISQAELDTAVKELEAAQAQVELVNA
                     QVARDKTNLRYTVIRSPISGVVVARNVELGQTVAASFNTPTLFQIAKDLAQMQIETSI
                     AEADIGGIKEGLAARFRVDAFPGQFFRGVVKQVRLNPTIQSNVVSYTVIVSADNAEGK
                     LKPGMTAFVQITANRREGVLRVPNAALRFRPPADPAQADKSATAARPTGGPGLGGGGG
                     GTGGAGIRAGRRDAGSRVYKIVDGKLTPVPVQVGISDTAYTEVLGGSLAEGDKLVTRD
                     LAGQGGTPSQLRLRMF"
     gene            4855..5568
                     /locus_tag="VFV55_07635"
     CDS             4855..5568
                     /locus_tag="VFV55_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944211.1"
                     /translation="MASRFIEVEDLGKQYEASAPGTPPALQSVSVSIGQGEFVAVMGP
                     SGSGKSTFMNIIGLLDTPTGGAYRLNGEDVARLSTDELAHVRNRVFGFVFQGFNLLKR
                     ISAAENVALPMVYAGARSGERRKRATEMLEKVGLAPYADAMPNRLSGGQQQRVAIARA
                     LANHPQLILADEPTGNLDTQTSNEIMSLFTALNRETGVTVLLVTHENDIAQYADRLIR
                     FKDGRVVHDGPVVHEAAAE"
     gene            5565..6788
                     /locus_tag="VFV55_07640"
     CDS             5565..6788
                     /locus_tag="VFV55_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944212.1"
                     /translation="MNFLSIIREAARSLGANKLRTGLTMLGMIIGVSAVVLMLAVGTG
                     AQDQVNRAVSSMGSNLLVILSGSTSAGTVRFGSGNAPTLTTGDAEAIARLPNVQAVAP
                     TFPGGAQVAYGINNWGTQVLGVTPEYTSVRDWPVESGDAISDTDIRSSTRVALLGSTV
                     VEKLFDTEDPVGKTIRIRGQPFQVMGVLRKKGQSLDGRDQDDSVLVPITTAQSKLFGN
                     RFPGTVRFMFVQAVSQEATSAAENDITLLLRQRHRIQPDADDDFTVRNLTQVAEAASA
                     TARILSLMLGAIGSISLVVGGIGIMNIMLVSVTERTREIGIRMAIGARQRDVLMQFLL
                     EALMICIVGGLIGIAIGVAGAFAIGKVMQIEVAFSAWVMLVAFAVSAATGIFFGFWPA
                     RRAASLRPVEALRYE"
     gene            complement(6779..>8503)
                     /locus_tag="VFV55_07645"
     CDS             complement(6779..>8503)
                     /locus_tag="VFV55_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944213.1"
                     /translation="LGLRAFGREAIAGVLVLALLAAVFDVDPRRSSSGVFRTGMAKLT
                     PKDSIIFHRDGKTATVDVLENQGSLSIRTNGKPDASISVGAGRPPTGDEFTMALLGVL
                     PLGHRPDAKTAAVVGFGSGMTSAILLASPRIERVDSIEIEPAMVEGAQLFRPAVEAAF
                     ADPRSHIVIDDAKSYFARGGGRYDIIVSEPSNPWVSGVASLFTEEFYARLALHLNEGG
                     VLSQWLHMYEMDATTLASIIQAVSRTFPQFMIYSSIDSDIVLIARKGGEPGRFDPAVL
                     RFEALRPVLDKLKLVDPAIVERRAVAGSDLVGPFFRLYGAPANSDFFPFVDKRATKTR
                     FTGSRVDELTQLQASAVPMLEMLGAGPMPSRERHDALLRTVFVEAAVDGAWMMHDVVT
                     ASGPPRAPTGELSVPELAAGAVRSWVTSCRDGSEFDRVLPSLVAAAVTANPYLHPDAA
                     MQIWRTVGESRCAKALAPPERRWIDLFEAVAQRDAARMAEQGGAILETIRGSKSSSSE
                     YAFFATVTALACQGQAARARELLGQARRNWVRDGVRSAELQFLGAVIASPPAPGKRAA
                     TCAAAVHS"
BASE COUNT         1397 a         3053 c         2782 g         1271 t
ORIGIN      
        1 ccggcacgct catcggcacc gccgtggtgg acaacctggg caactacctg ttcgaccagg
       61 tcctgaccag caccgtcggc gtgcagaacc cgaccaactc gctcgggaac ggcacggggt
      121 tctggtgctc cccgccgaag acgatgcgga tcacgtcttc gctcagcacc gccacggcca
      181 gcccgcccat ccagatcaag taatcctgga aaagcgtctg acctagcagc aagcagcaag
      241 gcaacaagga ccggtggcga aagccaccgg tccttttttt gttcgacgtg ttcgtgcaat
      301 ccggctgcgg cggcgtgccg tgccagacgc tactctcgtc ccacccagtg ccccgacttt
      361 ccgccttcct tctcgagaag gcgtatgtcg cccatcgtca tgccgcggtc gacggccttg
      421 cacatgtcgt agatcgtgag aagccccgcg ctcaccgcag ccagggcctc catctccacg
      481 cccgtgcggc cacgacattc ggcaacggcc cgacacgcaa caagcgacga gccttcatcg
      541 acgtcgaagg agacctcgag cgctgtcagc gcgatgggat ggcacagcgg aatcagctcc
      601 catgtccgct tggcagcctg gatggccgcg atgcgcgcca cccccaggac atcgcccttc
      661 ttcgcggttc cggagcggat gagagccagc gtttcgggac gcatgcggat cacgccggat
      721 gccacggcgc ggcgcgcggt ctcgtccttg ccgccgacgt cgaccatcca cgcccggccg
      781 ccttcgtcga aatgggtcag cttttcgttc ataatcaggg actccgcatg cggcgaacca
      841 ttgccgccgc catctgtccc aagtatccac gatttcattt ccccccgctc ccgcccccgg
      901 cccatgcttc gccgcctcgt cgccctcgcc ctctgcgccg ccttgctgcc cgcgcaggcg
      961 cagggcctgc cggacctcgg ggattcttcc gacgcggtgc tgtcggacaa ccaggagcgc
     1021 acgatcggca accgcgtgat gcgcgaagtg cgcatcgatc ccgcgttcgt cgacgatccg
     1081 gagatttccg attacctgaa atccctcggc aaccggctgc tcggcgccgc ggacccgccg
     1141 cgccgccaga tcgagttctt cgtggtgcag gacgacacca tcaacgcctt tgcgctgatc
     1201 ggcgggcaca tcggcgtgca cacggggctc attctcctga cgcgcaacga agcggaactc
     1261 tcgggcgtgg tcgcccatga gatcgcgcac atcctgcaac gccaccagtc gcgcctgtac
     1321 caggggcagg ggaaatacca gcttgcctcg cttgccgcgc tcgccctggc gatcctcgcc
     1381 tcgcgcggca gcagttcgca aagcggccag gtcaccgagg ccgcgatggt gggcgcaagc
     1441 gccatcatga tgcagggcca gctcgactac acgcgcgagc acgaacgcga ggccgaccgc
     1501 gtcggagtca cgctcctgga gcgcgccggc tacgacccgc gcggcatggt gagcttcttc
     1561 gagcgcatgc agcgcgccaa ccggctgagc gaattcaagg gcgcgccctc gtacctgcgc
     1621 actcacccgc tcaccgtcga gcgcatcgcc gacatgcaga gccgcgtgga ggccatgccc
     1681 gcacggctcg tcccggacag cttcgaatac cgcatcgcgc gcgcgaagat ccgcgccggc
     1741 tcggggtccg cgacggaagc ggtgacgtat ttccgcaacc agctcgcgga ccgttcggtg
     1801 ctgcgccccc gagaggacgt ttacggcctc gcgctcgcgt tgcggcgcac gcgcgacttc
     1861 gtcgaggccg agaaggaact ggccaccatc cgcggcagcg aaggcagcca tcccgccttc
     1921 gaatggctgg ccgcgcagct tcaggccgac caggggcgca tggacgccgc cctcgcgacc
     1981 tgccgggccg cgctcaagtc ccatccccag taccgcgggc tcgtctatgg ccttgcgcag
     2041 ttgctgctcg acgccggccg caaggccgaa gccatcgcat ggctccagga gaaggtccgc
     2101 gcctcgcccg aggacgccag gctctacgaa ctgcagtcgc gcgcctacgc ggcatcgggc
     2161 aaccgcctgg cccagcaccg tgcccaggcc gaggcctact tccagcgcgg caatctcgcc
     2221 gcggcggtcg accagctcga gctcgccacc aaggtgcgcg actcggactt ctacgagctt
     2281 tcgatggcgg aggcacggct gcgcgagctg cgctcccagc tcgcaaacga acgcgaggcc
     2341 gaaaaggccc tgaagctcag ctgatgagcc ggcgcgtgcg agctctcgcc gcggccgcgg
     2401 cactggccgc gggagccgcg tgggggcaag cgaccgaagg tcgagacctc ttcgcccgcc
     2461 ccgacaaggg caattgcatc gcctgccacc aggtcccgga tggttggggc gccgcggttc
     2521 gcgcggatgt gggtgcgcgc cttgatgccg cgcggctcaa ggggtgggat cgtgcccggt
     2581 tgcgcgatct gctcgacgac cccatgcggg ccaacccgga taccgtgatg ccgccctacg
     2641 ggcgccaccg catcctcgac gcgcgcgaga tcgagagcct catcgactac ctccatgcgc
     2701 ttccctgaac gccgattgcg cattgcattg cttttcgccg tcgccctgcc gctgcacgca
     2761 tgggccggcg cggagagcgt gcgcaccgac atcgtcaatc gcattcgcag ccaatatccc
     2821 gccctcgcgc cggcggatct cggcgccggc tcagcggcat tcgattccgc gcgcctcgcc
     2881 gaggccgaac gcaacaggcc cgcgcccggt gcagcggagg cggtcgatgc gggccggcgg
     2941 atctggacgc gcaagttcgc caagggccgg tcgctcgccg gctgcttccc caacggcggg
     3001 cgtcgcgtcg ccgcggcgta cccgcagtac gaccagcgcg tgaagggcct cgtgacgctg
     3061 gagaccgcga tcaaccagtg cctgaagacg cacgggcagc ccttgctcga gcctggcgat
     3121 cccgacacga tgggcgtggt cctcgcgtac ctgcgcacgc tctcgaccgg acagaagatc
     3181 gcggtgcgcg tggcctcggc acaggccgag gctcgcttcg aggagggtcg ccgcctctac
     3241 ttcacgcgca tgggccagca gaactattcc tgcgcgtcgt gccacgtgaa ccatgccggg
     3301 aaattcttcg gcgatgccgc cattcctgcg ccggtaggcg ccgcggcgca gtggccctat
     3361 ctgcgcgacg gccgccccgt gacgctgcag atgcgcattc gcgagtgcct cgatcgcatg
     3421 ggcgccgcgc cgtttcccgc cggatccgac gaactggcgc acctcgatta ttttctttcc
     3481 tacctctcca acggcctggt ggtccggccg aacgaatggc gcccgcacct ggtacacggc
     3541 aacttgtaac cgatcgtaag ggacggcctt gcacgcgcgc ccgcactcct agaatgaagg
     3601 cctgcaaagg gccctacgcc aaatgaccag caagctcctg cgcccgtccg ttctcgttgc
     3661 gatcctcgtc cttgcggccg ccggcggtta cgcctggtgg cagtacggtt ccggcacaac
     3721 acccgcggag cgctggcgac tcgaggcggt cgacaagggt gacgtggtgc aggtgatctc
     3781 ggccaacggc aacgtgaacc ccgtgacctc ggtcagcgtg ggcacgcagg tgtccggcac
     3841 cctcatcaag ctgcatgccg attacaacag cgtggtcaag gaaggccagc tcctcgcgga
     3901 actcgatccg gcgctcttca acgccgcgct cgcccagagc gaggccaacc tcaacagcgc
     3961 ccgcgcgtcg ctgcgcctgg ccgagaccaa ggaagcgcgc acgcgctcgc tggtggccaa
     4021 gggattcatc tcgcaggcgg aactcgatac cgcggtgaag gaactcgagg ccgcccaggc
     4081 ccaggtcgag ctcgtgaatg cccaggttgc gcgcgacaag accaacctgc gctacacggt
     4141 gatccgctcg cccatatccg gcgtggtggt cgcgcgcaac gtcgagctgg gccagacggt
     4201 ggcggccagc ttcaacacgc ccacgctctt ccagatcgcc aaggacctcg cgcagatgca
     4261 gatcgagacg agcatcgcgg aagccgacat cggcggcatc aaggaggggc tggccgcccg
     4321 ctttcgcgtg gatgcgttcc ccggccagtt cttccgcggc gtcgtgaagc aggtccggct
     4381 caatcccacg atccagtcca acgtcgtgag ctacacggtc atcgtgagcg ccgacaacgc
     4441 cgaaggaaaa ctcaaacccg gcatgacggc cttcgtgcag atcacggcca atcgtcgcga
     4501 aggcgtgctt cgcgtgccca acgcagcgct gcgcttccgc ccgccggccg atccggccca
     4561 ggccgacaag tccgcaaccg ccgcccggcc caccggtggc cccggactag gtggaggggg
     4621 cggaggaacc ggtggagcag gcatccgggc ggggcgccgc gacgcgggct cgcgcgtcta
     4681 caagattgtc gacgggaagc tcacgccggt gcccgtgcag gtcggcatct ccgacaccgc
     4741 ttacaccgag gtgctgggcg gctccctcgc ggagggcgac aagctcgtca cgcgcgacct
     4801 cgccggccag ggcggcacgc cgtcgcaact gcgcctgcgc atgttctagc ctccatggcc
     4861 agccgcttca tcgaggtcga ggacctcggc aagcagtacg aggcctcggc gccgggaacg
     4921 ccgccggccc tgcagtcggt atccgtttcc atcggccagg gcgagttcgt cgcggtgatg
     4981 gggccctcgg gctcgggcaa gtcgaccttc atgaacatca tcgggctgct cgacacgccc
     5041 accggtggtg cctatcgcct caacggagag gacgttgcgc ggctatccac cgacgagctg
     5101 gcgcacgtgc gcaaccgcgt cttcggcttc gtcttccagg gcttcaacct gctcaagcgc
     5161 atcagcgcgg ccgagaacgt ggcgctgccg atggtctatg caggcgcccg cagcggcgag
     5221 cggcgcaagc gcgcgacgga aatgctggag aaggtgggcc tggccccgta tgccgacgcg
     5281 atgcccaatc ggctctcggg cggccagcag cagcgcgtgg ccatcgcccg cgcgctcgcg
     5341 aaccacccgc agctgatcct ggccgacgag ccgacgggca acctcgacac gcagacgagc
     5401 aacgagatca tgagcctctt taccgcgctc aaccgcgaga ccggcgtcac ggtactgctc
     5461 gtcacgcacg agaacgacat cgcgcagtac gccgaccggc tcatccgctt caaggacggg
     5521 cgcgtggtgc acgacggccc ggtcgtgcac gaggccgcgg cggaatgaac ttcctgtcca
     5581 tcatccgcga ggcggcccgc tcgctcggcg ccaacaagct gcgtaccggc ctcaccatgc
     5641 tcggcatgat catcggcgtc tccgccgtgg tgctcatgct cgcggtgggc acgggtgcgc
     5701 aggaccaggt gaaccgcgcc gtgtcctcga tgggcagcaa cctgctggtg atcctctccg
     5761 gaagcaccag cgccggcacg gtgcgcttcg gttccggaaa tgcgcccacc ctcaccacgg
     5821 gcgacgcgga ggccatcgcg cgcctgccca acgtgcaggc ggtggcgccc acgtttcccg
     5881 gcggcgcgca ggtggcctac ggcatcaaca actggggcac gcaggtgctc ggcgtgacgc
     5941 ccgaatacac ctcggttcgc gactggccgg tcgagtccgg cgacgcgatc agcgacaccg
     6001 acatccgcag ttccacgcgc gtggccctgc tcggctcgac ggtggtcgaa aagctcttcg
     6061 acaccgagga ccccgtcggc aagaccatcc gcatccgcgg gcagcccttc caggtgatgg
     6121 gcgtgctccg gaagaagggc cagagcctgg acgggcgcga ccaggacgat tcggtgctgg
     6181 tcccgatcac gaccgcgcag agcaagctct tcggcaaccg cttccccggg acggtgcgct
     6241 tcatgttcgt gcaggcggtg agccaggagg ccacgagcgc ggcggaaaac gacatcaccc
     6301 tgctgcttcg ccagcggcac cgcatccagc ccgacgcgga cgatgatttc acggtgcgca
     6361 acctcacgca ggtcgcggag gcggcctcgg ccacggcgcg catcctctcg ctcatgctcg
     6421 gggccatcgg ctcgatctcg ctcgtcgtgg gcggcatcgg gatcatgaac atcatgctgg
     6481 tgtcggtcac cgagcgcacg cgcgagatcg gcatccgcat ggcgatcggc gcgcgccagc
     6541 gcgacgtgct catgcagttc ctgctcgagg cgctgatgat ctgcatcgtg ggcggcctca
     6601 tcggcatcgc gatcggcgtg gccggcgcct tcgcgatcgg caaggtcatg cagatcgagg
     6661 tggcgttctc ggcgtgggtg atgctggtgg ccttcgccgt gtcggcggcc accgggatct
     6721 tcttcggctt ctggccggcg cggcgggcgg cgagcctgcg gccggtggag gcgctgcgct
     6781 acgagtgaac ggccgcggcg cacgtcgccg cgcgctttcc cggagcaggg ggcgacgcaa
     6841 tcacggcgcc caggaattgc agctccgcgg accgcacgcc atcgcgcacc cagttccgcc
     6901 gtgcctgccc gagcagctcg cgcgcgcgcg ccgcctgtcc ctggcaagcc agcgccgtga
     6961 ccgtcgcgaa gaacgcgtac tcgctcgacg acgacttcga cccccggatc gtctccagga
     7021 tcgcgccgcc ttgctcggcc attcgcgcgg cgtcgcgctg cgccaccgcc tcgaacaagt
     7081 cgatccagcg acgttccggc ggcgcaagcg ccttcgcaca gcgtgattcg cccaccgtgc
     7141 gccagatctg catggcggcg tcggggtgca ggtagggatt ggccgtcacg gccgccgcga
     7201 cgagggatgg caggacccgg tcgaattcgg aaccgtcccg gcaactcgtc acccaggacc
     7261 gaacggcgcc tgccgccagt tcgggcacgc tcagctcgcc ggtcggcgcg cgcggcggac
     7321 ccgatgcggt cacgacgtcg tgcatcatcc acgccccatc caccgccgcc tcgacgaaca
     7381 ccgtgcgcaa cagggcgtcg tggcgttcgc gcgacggcat cgggcccgcg ccaagcatct
     7441 ccagcatcgg cacggcggag gcctgcaatt gcgtcagttc gtcgacccgt gagccggtga
     7501 agcgtgtctt cgtggcgcgc ttgtccacga acgggaagaa gtccgaattc gcgggcgcgc
     7561 cgtagaggcg aaagaacggg ccgaccaggt ccgacccggc gaccgcgcgc cgctccacga
     7621 ttgcgggatc gaccagcttc agcttgtcga gcacggggcg caacgcctcg aagcgcagca
     7681 cggcgggatc gaagcggccc ggctccccgc ccttcctcgc gatgaggacg atgtcagagt
     7741 cgatcgagga atagatcatg aactgcggaa aggtccgcga cacggcctgg atgatggagg
     7801 caagggtcgt cgcgtccatc tcgtacatgt gcagccactg cgacagcacg ccgccctcgt
     7861 tcaggtgcag ggccagccgc gcgtagaact cctcggtgaa gagggacgcc acgccgctca
     7921 cccacggatt ggagggctcc gacacgatga tgtcgtagcg tccgccgccg cgcgcgaagt
     7981 acgatttcgc gtcgtcgatc acgatgtggc tgcgcgggtc ggcgaacgcc gcctccacgg
     8041 caggacggaa gagctgcgcc ccctcgacca tggccggctc gatctcgatg gaatcgacgc
     8101 gctcgatgcg cggcgaggcg agcagtatgg cgctcgtcat cccggagccg aatcccacca
     8161 ccgccgcggt cttcgcgtcg ggacgatgac ccaacggcag cacgccgagc agtgccatgg
     8221 tgaactcgtc cccggtcggc ggcctgcccg cgcccacgga aatgctcgcg tcgggcttgc
     8281 cgttcgtccg gatcgagagc gaaccctggt tttccaggac atccacggtc gccgtctttc
     8341 cgtcgcggtg gaagatgatg gaatccttcg gggtcagctt cgccatcccc gtccggaaga
     8401 cgccggacga cgaacgccgc gggtccacgt cgaacacggc ggcgaggagc gccagcacca
     8461 ggacccccgc aatcgcctcg cggccgaagg ccctcaggcc cag
//
LOCUS       DASUEV010000235         6760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_638238, whole genome shotgun sequence.
ACCESSION   DASUEV010000235 DASUEV010000000
VERSION     DASUEV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638238"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VFV55_07650"
     CDS             <1..1707
                     /locus_tag="VFV55_07650"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX4944214.1"
                     /translation="VIHASLPPRLRQAKRRAIKAPPGAPSAVGPFPSPVTATPAQEEA
                     LARIREGFGRFHPVLLSGITGSGKTEVYLRLIDDVLAQGRQALYLVPEIALTPQLETR
                     VRSRFPGVAIVAAHSHLGEGERAGAWLAAQSGRARIVLGTRLAALSPMPELGLIVVDE
                     EHDPSYKQHEGLRYSARDVAVRRAQMVAIPVVLGSATPSLETWANAKQGRYALVELKE
                     RALAGAKLPVVRMVDTRADPPVDGVTDALLRALRSRIAKGEQSLVFLNRRGYSPVLFC
                     RSCAWHSACSRCSANLVLHQKAGELRCHHCGHRERVPARCPSCGSHDLAPVGHGTQRI
                     EETLAAALPGARIARVDRDSTSRKGALAEVLEQVRDERIDVLVGTQMLAKGHDYPRLT
                     LVGAVDSDCALYSVDFRAAERLFSLLTQVAGRAGRAEQPGEVLIQTDFPTHPLYAAVA
                     AHDYAAFADAALEERRLAQFPPFAHIAMLRAESKHAGEAAAFLRAAARLARAIGGKVE
                     IFDPVPAPLERKAGFERAQLLVRAASRQALQPFLRRWKSALDDKAERRVRWSLDVDPQ
                     EA"
     gene            1752..3407
                     /gene="argS"
                     /locus_tag="VFV55_07655"
     CDS             1752..3407
                     /gene="argS"
                     /locus_tag="VFV55_07655"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX4944215.1"
                     /translation="MTEADTKTRLAALVTLAVRTAYPEAGDVTVELDRPRNADHGDFA
                     CNVALQLANRVGRKPREAAQAIVAALGNAPEIAKAEIAGPGFINITLSRASRFGVVGA
                     ILSAAGDFGRGRSGEGRTIIVEFVSANPTGPLHVGHGRQAALGDALSTILEWQRWKVL
                     REFYYNDAGNQIANLAFSVQFRIREARGIAASTEMHDDWYRGDYIKDIARDYVAEYPN
                     DANGDDLEAIRRFAVAYLRGEQDADLRAFGVKFDSYYLESSLYTDGKVEETVKMLVAA
                     GRTYEAEGALWLRTTDFGDDKDRVMRKSDGTYTYFVPDVAYHLTKFRRGYATAVNVQG
                     SDHHSTVTRVRAGLQALAAGIPEGYPQYVLHQMVTVMKGGEEVKISKRAGSYVTVRDL
                     VEEAGRDAVRWFFIMRKSDSHLVFDIDLAKSQSDENPVYYVQYAHARICSVLREWGGD
                     EASLRGADLSVLGTPHEVALAQALAQFPVVLERAAREFSPHLVSFYLHDELAARLHTY
                     YNAERFLVEDGKMKHARLALVAATRQVLASGLAVLGVSAPERM"
     gene            3408..4079
                     /locus_tag="VFV55_07660"
     CDS             3408..4079
                     /locus_tag="VFV55_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4944216.1"
                     /translation="MPKDFKNNPPPRDNARAGHPLLLGMIIGLLMGIVIALSVALWLN
                     RLSNPFVDKAKSFEPLAKIGPAQPPKPDEKGAAAKGAADKAPAEKPKTDRPRFEFYTM
                     LPGEKEVTDKEAKAAVAKPKEPAKSGPGSSPSQPKPHSGEVFWLQAGAFAEEKDADNL
                     KAKIAFTGLEASVRPVEVPGKGTLHRVRLGPYQSLEDANRFKTALSQNGVDATIIRTD
                     EAKNR"
     gene            4092..4778
                     /locus_tag="VFV55_07665"
     CDS             4092..4778
                     /locus_tag="VFV55_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX4944217.1"
                     /translation="MSLRRILTLAIATLGFLAGGAHAQLRQGQEFTLLTPAQPTDGGG
                     KVEVIEFFSYACGHCNRLEPFLESWAKKLPADVVFKRIPGAGSESWSQLALLFYSLEA
                     MGKLDALHAKAFDAMHKDNLNLALPKVREQWMIKMGIDPAQYNAVEKSFTVQSKLARA
                     KQLMGAYKVDGVPMLIVNGKYVTSTGHAGSPERVVPVVEELIAMARKDMGTSTAEAPK
                     AAAKPAAVKK"
     gene            4794..5534
                     /locus_tag="VFV55_07670"
     CDS             4794..5534
                     /locus_tag="VFV55_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4944218.1"
                     /translation="MTGASSGIGEALARQYAARGATLGLVARRAELLDALRRSLPSEA
                     ETYAADVRDPGALKAAAADFIDKHGVPDLVIANAGISHGTLTEHAEDLDVFREILDVN
                     VVGMVNTFHPFVAAMREAGRGTLVGVASLAGYRGLPGIGAYSASKAGAIRYLESLRVE
                     LRGTGVKVVTICPGYIATPMTERNPYPMPFIITADDFAQRFARVVGGGRNYAVIPWQM
                     GLVAKFLTMLPNSWFDAAFARTERKPRK"
     gene            complement(5584..>6760)
                     /locus_tag="VFV55_07675"
     CDS             complement(5584..>6760)
                     /locus_tag="VFV55_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX4944219.1"
                     /translation="MYATYRMARLRNQVLDFSDALNAMRERVLVAAAKNVNTIVPAYT
                     NGVQAQPITYAHYLLAYEASFDRDAQRIRELYKRLNRSAMGTAVLANSSWPLNRARLA
                     ELLGFDGVIENSLDSSQVSPSDIALEASGIASSTAIRLGAILGDIHTQYHQTRPWLLL
                     EEGATYTSSAMPQKRNPGLIMRAREAASNVVGLAHTVTIRAHNVTTGMTDYKEPAAEI
                     GLFPQAVRMVTAFNAVLDALKVNASRSLDELEDDWTASMELAETLQKDHQIPFRVGHS
                     FASSIVTVARASAIRPRDFPYAKAAELYAQAITKYKLPDTQLPIKEAELRQVLSAKYM
                     VESRVGTGGPQPAEVERMLAGAQATLKGDKAWMQKARQNLKDADATLDKAFAQLLAE"
BASE COUNT         1081 a         2359 c         2279 g         1041 t
ORIGIN      
        1 gtgattcacg cgagccttcc gcccaggctt cgccaggcga agcgccgcgc gatcaaggcg
       61 cctccggggg caccctccgc cgtgggcccc tttccgtcgc ccgtcacggc aacacccgcg
      121 caagaggaag ccctggcgcg aatccgggaa ggcttcgggc gcttccaccc ggtgctcctt
      181 tccggcatca ccggcagcgg caagacggag gtgtacctgc ggctcatcga tgacgtgctg
      241 gcgcaggggc ggcaggcact ctacctggtg ccggagatcg cgctcacccc gcagctcgag
      301 acgcgcgtgc gcagccgctt ccccggcgtg gcgatcgtcg cggcacacag ccacctgggc
      361 gagggcgagc gtgcaggcgc gtggctcgcg gcgcaatcgg gccgggcgcg catcgtgctg
      421 ggcacgcggc tcgccgcgct atcgccgatg ccggagctgg gactcatcgt ggtggacgag
      481 gagcacgacc cctcctacaa gcagcatgag gggcttcgct actccgcgcg agatgtcgcg
      541 gtgcgccgtg cccagatggt ggcgataccc gtggtgctcg gctcggccac gccgtcgctc
      601 gagacctggg ccaacgcgaa gcagggccgc tacgccctcg tggagctgaa ggaacgggca
      661 ctcgcgggcg cgaagctgcc cgtggtgcgc atggtcgaca cgcgcgccga cccgcccgtg
      721 gacggcgtga ccgatgcgct gctgcgggcg ttgcgcagcc ggatcgcgaa gggcgagcag
      781 agcctcgtgt tcctcaatcg ccgcggctat tccccggtgc tcttttgccg ctcgtgcgcc
      841 tggcattcgg cgtgcagccg ctgcagcgcc aacctggtgc tgcaccagaa ggcgggcgag
      901 ctgcgctgcc accactgcgg ccaccgcgag cgggtgcccg cgcgctgccc gtcctgcggc
      961 agccacgacc tcgcacccgt gggccatggg acgcagcgca tcgaggagac gcttgccgcc
     1021 gcgctgcccg gcgctcgcat tgcgcgggtc gatcgcgatt ccacctcgcg caagggcgcc
     1081 ctggcggagg tgctggagca ggtgcgcgac gagaggatcg acgtcctcgt gggcacgcag
     1141 atgctcgcca agggccacga ctacccgcgc ctcacgctgg tgggcgcggt cgacagcgac
     1201 tgcgcgctgt acagcgtgga tttccgcgcg gccgagcggc tcttctcgct cctcacccag
     1261 gtggccgggc gcgcgggccg cgcggaacag ccgggagagg tgctcatcca gaccgacttc
     1321 cccacccacc cgctctacgc agccgtcgcc gcacacgact acgccgcttt cgccgacgcg
     1381 gcgctggagg agcgacgcct cgcgcagttc ccgccgttcg cgcacatcgc gatgctgcgc
     1441 gcggaatcga agcacgcggg cgaggcggcc gcgttcctgc gcgcggctgc aaggctcgca
     1501 cgtgcaatcg gcggcaaggt ggaaatcttc gaccccgtgc ccgcgccgct ggaacgcaag
     1561 gccgggttcg agcgcgccca gctcctcgtg cgcgccgcat cgcgccaggc gctgcagccc
     1621 ttcctgcgcc gctggaagtc ggctctcgac gacaaggccg agaggcgcgt gcgctggtct
     1681 ctcgacgtcg acccccagga ggcgtgaaaa ggcacccgta taataagggc cattcgcagg
     1741 atcccgcccc agtgaccgaa gccgacacca agacccgcct cgccgccctt gtgaccctgg
     1801 ccgtgcgcac ggcctatccc gaagccgggg atgtgacggt cgaactcgac cgccccagga
     1861 acgccgacca cggcgacttc gcctgcaacg tggccctgca gctcgccaat cgcgtgggaa
     1921 ggaagccgcg cgaagccgcg caggccatcg ttgccgcact cggcaatgca ccggagatcg
     1981 cgaaggccga gatcgccggc cccgggttca tcaacatcac gctttcccgg gcctcgcgct
     2041 tcggcgtggt gggcgcgatc ctttccgccg ccggcgactt cggccgcggc cgctcgggcg
     2101 agggcaggac catcatcgtc gagttcgtct cggccaaccc cacgggcccc ttgcacgtgg
     2161 gccacggccg ccaggcggcg ctgggcgatg ccctctccac gatcctcgag tggcagcgct
     2221 ggaaggtgct gcgcgagttc tactacaacg acgcgggcaa ccagatcgcg aacctggcct
     2281 tctccgtgca gttccgcatc cgcgaggcgc gcggcatcgc cgcgagcacg gagatgcacg
     2341 acgactggta ccgcggcgat tacatcaagg acatcgcgcg cgattacgtg gccgagtacc
     2401 cgaatgatgc gaacggcgac gacctcgagg ccatccgccg cttcgcggtc gcctatctcc
     2461 ggggcgagca ggatgcggac ctgcgcgcct tcggcgtgaa gttcgactcc tactacctcg
     2521 agagctcgct ctacaccgac ggcaaggtcg aggagacggt aaagatgctt gtggccgcgg
     2581 gaaggaccta cgaggccgag ggcgccctgt ggctcaggac caccgacttc ggcgacgaca
     2641 aggaccgcgt gatgcgcaag tcggacggca cctacacgta cttcgtgccg gacgtggcct
     2701 atcacctcac caagttccgc cgcggctacg caacggccgt gaacgtgcag ggctcggacc
     2761 accactcgac ggtgacgcgc gtgcgcgcgg gcctgcaggc gctggccgcg ggcatccccg
     2821 agggttaccc gcaatacgtg ctgcaccaga tggtgacggt gatgaagggc ggcgaggagg
     2881 tgaagatctc caagcgcgcc ggctcctacg tcaccgtgcg cgacctggtc gaggaggcgg
     2941 gccgcgacgc cgtgcgctgg ttcttcatca tgcgcaagtc cgattcgcac ctcgtcttcg
     3001 acatcgacct cgccaagagc cagagcgacg agaacccggt gtactacgtg cagtacgcgc
     3061 acgcgcgcat ctgctcggtg ctgcgcgagt ggggcggcga cgaggcttcg ctgcgcggcg
     3121 ccgacctctc cgtgctcggc acgccgcacg aggtggcgct ggcccaggcg ctcgcgcagt
     3181 tcccggtggt gctcgagcgt gcggcgcgcg aattctcgcc gcacctggtc tcgttctacc
     3241 tgcacgacga attggcggca cgattgcata cctactacaa tgccgagcgc tttctcgtcg
     3301 aggacgggaa gatgaagcac gcgcggctgg cgctcgtcgc cgcgacgcga caggttctcg
     3361 ccagcggcct cgccgttctt ggcgtcagcg cgccggagag gatgtagatg ccgaaggact
     3421 tcaagaacaa cccgccgccc cgcgacaacg cccgcgcggg ccaccccctg ctgctcggca
     3481 tgatcatcgg cctgctcatg ggcatcgtca tcgcgctttc ggtggcgctg tggctcaacc
     3541 gcctgtcgaa cccgttcgtc gacaaggcga agtccttcga gccgctggcg aagatcggtc
     3601 ccgcgcagcc gccgaagccc gacgagaagg gcgcggcagc caagggcgcg gccgacaagg
     3661 cccccgcgga gaagccgaag accgaccgac cgcgcttcga gttctacacc atgctgcccg
     3721 gcgagaagga agtgacggac aaggaggcga aggccgccgt cgcgaagccg aaggagcccg
     3781 ccaagagcgg ccccggctcc tcgcccagcc agcccaagcc gcactccggc gaggtcttct
     3841 ggcttcaggc cggcgcgttc gccgaggaga aggacgccga caacctgaag gcgaagatcg
     3901 ccttcacggg cctggaggcc tcggtacggc ctgtcgaggt gccgggcaag ggtacccttc
     3961 accgcgtccg gctgggcccc taccagagtc tcgaggacgc gaaccgattc aagaccgcgt
     4021 tgtcccagaa cggtgtcgat gcaaccatca tccgcaccga cgaagccaag aaccgctaac
     4081 ggagtcccga catgtcattg cgccgtatcc ttacccttgc catcgccacc ctgggttttc
     4141 tggccggggg cgcccacgcc cagctccgcc aggggcagga attcaccctc ctcacccccg
     4201 cgcagccgac cgatggcggc ggcaaggtcg aggtgatcga gttcttctcc tacgcgtgcg
     4261 gccattgcaa ccggctcgag cccttcctgg aaagctgggc gaagaagctg cccgcggacg
     4321 tggtcttcaa gcgcatcccc ggtgccggca gcgagtcctg gtcgcagctg gcgctgctct
     4381 tctactcgct cgaggcgatg ggcaagctcg acgcgctgca cgccaaggcg ttcgacgcca
     4441 tgcacaagga caatctcaac ctcgccctcc cgaaagtgcg cgagcagtgg atgatcaaga
     4501 tgggcatcga cccggcgcag tacaacgcgg tcgagaaatc gttcaccgtg caatcgaagc
     4561 tcgcgcgcgc caagcagctc atgggcgcct acaaggtgga cggcgtgccg atgctcatcg
     4621 tgaacggcaa gtacgtcacc tccaccggcc atgccggcag cccggagcgc gtggtaccgg
     4681 tggtcgagga actcatcgcc atggcgcgca aggacatggg cacgagcact gccgaggcgc
     4741 ccaaggcggc ggccaagccc gccgcggtca agaagtaggc attggtcgtc ttcctgaccg
     4801 gtgccagcag cggcatcggc gaggccctcg cgaggcaata cgctgcacgt ggcgcaacgc
     4861 tcggcctcgt cgcccgccgc gccgagctcc tcgatgccct tcgccggtcg ctgccttcgg
     4921 aggcggagac gtacgctgcg gatgtgcgcg accccggggc cctcaaggcc gcggcggccg
     4981 atttcatcga caagcacggc gtgccggacc tcgtgatcgc gaatgccggc atatcgcacg
     5041 gcaccctgac cgagcatgcc gaggacctcg acgtcttccg cgaaatcctc gacgtcaacg
     5101 tggtcggtat ggtgaacacc tttcatccct tcgtcgcagc catgcgcgag gccgggcgcg
     5161 ggacgttggt cggcgtggcg agcctggccg gctatcgcgg gctccccggt atcggagcct
     5221 acagcgcgtc gaaggcgggg gcgatccgct acctcgaaag cctgcgcgtg gagctgcgcg
     5281 gcactggcgt gaaggtggtg accatctgcc cgggctacat cgccacgccc atgaccgaga
     5341 ggaaccccta cccgatgccg ttcatcatca cggcggacga tttcgcgcag cgcttcgcgc
     5401 gtgtcgtcgg cggcggccgg aactacgcgg tcatcccctg gcagatgggg ctcgtcgcga
     5461 aatttctcac gatgcttccg aactcctggt tcgacgccgc gttcgcgcgg acggagcgca
     5521 agccgcgcaa gtgacgggga cggttccgga gaaccgtccc caggccaggc aggcgcgttc
     5581 ccgctactcg gccagcagct gggcgaatgc cttgtcgagc gtcgcgtccg cgtccttcag
     5641 gttctggcgc gccttctgca tccacgcctt gtcgcccttc agggtcgcct gcgccccggc
     5701 gagcatgcgc tccacctcgg ccggctgcgg gccgccggtt cccacgcgac tctccaccat
     5761 gtatttcgcc gacaggacct gccgcaactc ggcctccttg atcggaagct gcgtgtcggg
     5821 gagcttgtat ttcgtgatgg cctgggcgta gagctcggcg gccttcgcgt acgggaaatc
     5881 ccgcggcctg atcgcgctgg cgcgcgctac cgtcacgatc gacgacgcga agctgtggcc
     5941 gacgcggaac gggatctggt ggtccttctg cagcgtctcg gccagttcca tcgacgcggt
     6001 ccagtcgtcc tcgagctcgt ccagcgagcg gctggcgttc accttcagcg cgtccaggac
     6061 ggcattgaag gcggtgacca tcctcaccgc ctgcggaaac aggccgatct cggccgccgg
     6121 ttccttgtag tcggtcattc ccgtggtgac gttgtgcgcg cggatcgtga ccgtatgcgc
     6181 aagcccgacc acgttcgacg ccgcttcgcg cgcacgcatg atgagaccgg gattgcgctt
     6241 ctgcggcatg gcgctgctcg tgtaggtggc gccttcctcg agaagcagcc agggccgcgt
     6301 ctggtggtac tgcgtgtgga tgtcgcccag gatcgcgccc aggcgaatcg cggtcgagga
     6361 ggcgatgccc gacgcctcca gcgcgatgtc cgacgggctc acctggctcg agtccagcga
     6421 gttctcgatg acgccgtcga agccgaggag ttcggccaac cgggcgcggt tcagcggcca
     6481 gctcgagttt gcgagcaccg cggttcccat ggcgctgcga ttgagcctct tgtagagctc
     6541 gcggatgcgc tgcgcatcac gatcgaacga tgcctcgtac gccaacaggt aatgggcgta
     6601 ggtgatgggc tgggcctgga ccccgttggt ataggccggg acgatggtgt tcacgttttt
     6661 cgcggccgcc accagaacgc gctcgcgcat ggcgttcagc gcgtcggaga agtcgagcac
     6721 ctggttgcgc agcctcgcca tccggtaggt cgcatacatg
//
LOCUS       DASUEV010000236         9334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_874195, whole genome shotgun sequence.
ACCESSION   DASUEV010000236 DASUEV010000000
VERSION     DASUEV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874195"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VFV55_07680"
     CDS             <1..1511
                     /locus_tag="VFV55_07680"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX4944220.1"
                     /translation="AWSGAYAIFVAVCVWLAWRSRGLEPLAPVAAAAEEDTEPRPSAR
                     SLLVWLVLAAMGSVTLLAVSNHLTQNISSIPLLWVIPLAIYLLTFILCFEGTRWYRRD
                     AYLGLLVWIVCVMAWFLADKRLQFELAWQVAVFAAGLYIVCMFCHGELARLRPGPKYL
                     THFYLVVSLGGVVGGILVAIVAPLTLPGYLELEIALVVVALLALYLNLDRPKPVVAMF
                     AAVALFTAGALAYRIGNFTEDTVLIERNFYGVLRVKEQRSRDDDPETRYRSLVHGSIL
                     HGEQWMSDKYRRAATTYYKTSSGIGKAILAFDGRPIKVGVIGLGAGTLATYGDADDTY
                     RFYEINPAVPRIAREQFTFLGDSPARIEIVLGDARLSLEREPVQGFDVLAVDAFSGDS
                     IPVHLITLEAVGAFLRHMKPTGVIAFHVSNRFLDLKPVLLAIAERHGLEYAFVHETGD
                     DGGTTSDWVLLTRNKPLILRPEIVSATEPVAPQPDWRLWTDAYNNLLQVWRR"
     gene            complement(1508..2440)
                     /locus_tag="VFV55_07685"
     CDS             complement(1508..2440)
                     /locus_tag="VFV55_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX4944221.1"
                     /translation="MQRIVISEDMDAPAVAFLARDFDVDYRPALALDVAGLSNALAQA
                     DAWIVRNRTMVRGAALAAASRLRVVGRLGVGLDNIDLEACAARGIEVIPATGANAESV
                     AEYVLAMAMILLRGAAYLSTAAVAAGGWPRQKLSQGREISGKTLGLVGFGSIGRVTAR
                     KAIALGMRVIAHDPALPVAWPPWSELGVGPCGLDELLANADVVSLHLPLVDSTRGLIG
                     EDQFARMKPDAILVNSARGGIVDEAALARALHQGRLAGAALDVFADEPLGAGSVLADA
                     PNLILTPHIAGLTRESNERVSEIVARRVAACLRG"
     gene            complement(2537..3604)
                     /gene="rlmM"
                     /locus_tag="VFV55_07690"
     CDS             complement(2537..3604)
                     /gene="rlmM"
                     /locus_tag="VFV55_07690"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HEX4944222.1"
                     /translation="MSAANLLAYCRAGFEKECAQEMTALAGEAGVEGFVKARPDSAFA
                     VFHPHDATAGLGFARRLDATALVFPRQVVRCGEMLADLPVGDRVAPIVSAAAALGTSF
                     RETFLETPDTNDGKALASLIRPLEPHLLRALPKAGIAIDDPKAAGRLHVFFLGGAACY
                     VGTSRVGASCPWPMGIARIRMPGGAPSRSTLKLAEALALFIGEEALARRMRPGMTAVD
                     LGASPGGWTWQLVRRGLMVTAIDNGPMDAALMESGQVKHRREDGFRYRPPEPVDWMVC
                     DMVESPSRVCTLAAQWVAEGWCREAIFNLKLPMKKRWEEVARARGIIEEALAGVAYRL
                     RMKHLYHDREEITVWLAAGKR"
     gene            complement(3623..4402)
                     /locus_tag="VFV55_07695"
     CDS             complement(3623..4402)
                     /locus_tag="VFV55_07695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX4944223.1"
                     /translation="MLVDSHCHLDFPELRAELPALLARMAARGVTHALTISTTLAGFP
                     GVREVAESQPNLWCSAGVHPDEQRDGREATVEELVRLASHPKVVAIGETGLDYFRVEG
                     DTEWQRSRFRVHVRAARACAKPLVIHTREAAADTLRILEEERASEVGGVMHCFTESLE
                     VAQAAMRLGFLISFSGIVTFKNAQALKEVARHVPLESMLVETDAPYLAPVPHRGKTNE
                     PAFVSHVAEEIARLKGTSLEEVARATTRNFFALFRDARPAT"
     gene            complement(4445..4813)
                     /locus_tag="VFV55_07700"
     CDS             complement(4445..4813)
                     /locus_tag="VFV55_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4944224.1"
                     /translation="MNDIATDPAITRPAVFSLVIRSKAALYAAWIPLLRGGGIFVPST
                     REHNLGEEVLILLSLLDDSVKIPIHGHVAWVNCAHAASNRPQGFGVQLPNTETCRELK
                     KRIEGLLAGALQSTRQTHTI"
     gene            complement(4817..5821)
                     /gene="holB"
                     /locus_tag="VFV55_07705"
     CDS             complement(4817..5821)
                     /gene="holB"
                     /locus_tag="VFV55_07705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX4944225.1"
                     /translation="MNGLPWHREGLERLMGRRDRLPHALLVSGRPGIGKVEFAREFAR
                     SLLCESPIDRLACGQCPSCHWFGQSNHPDYREIVPESAEEGEGEEADAGRDAGKKSLV
                     IKIDQVRAIREFVSLSTHRAGHRAIVLHPAESLQPAAANALLKTLEEPPPATVLVLVS
                     DRPARLLATIRSRCESVVLRAPPREEALAWLTAGGAGEPEVSLALAGGAPLLAAELAQ
                     PAEREWRRKIVAELSRPGGAHVLAFAAGFDRVLLEPMLFVMQTWVHDLVRLKNQGEPR
                     HHVDCVPVLKGKARRARLDRLLALDRDLLEARRLVAHPLNARLAAEHLLMAYNQATLS
                     "
     gene            complement(5818..6435)
                     /gene="tmk"
                     /locus_tag="VFV55_07710"
     CDS             complement(5818..6435)
                     /gene="tmk"
                     /locus_tag="VFV55_07710"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX4944226.1"
                     /translation="MTAGRFITVEGIDGAGKSSHLEFLASAIRARGVEAVVTREPGGT
                     PLGEKLRELVLHQPMEGTTEALLMFAARREHLVRVIEPALARGAWVLSDRFSDATFAY
                     QCGGRGLDRGVFRALESIVHPRCNPDATFLFDLDPAVAMDRQRAQSRTPDRFEREATE
                     FFRRVRDAYLERARENPLRVRVIDASGGIEDVRARLASDFAKAFP"
     gene            complement(6432..7430)
                     /gene="mltG"
                     /locus_tag="VFV55_07715"
     CDS             complement(6432..7430)
                     /gene="mltG"
                     /locus_tag="VFV55_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX4944227.1"
                     /translation="MRAVRRLVLVSAAGLAIAALAWAAWFCLRPLEPPRVPFDFTVKS
                     GASLKTVARNLAEEGLLPEAQTFWLLGRILGKAGSLRAGTYRVASPLTPLELLDKLAS
                     GDVLPIEIVFVEGTTFRQWLAQMRSHPQVRSTLEGLSEDGIRAAIGLPGAALEGLFFP
                     DTYHFLSGTTDVVILKRAHAEMERRLARAWESRDPALPLASAYEMLILASIIEKETGQ
                     SGERPAIASVFVNRLRKGMRLQTDPAVIYGLGARFDGNLRKRDLLADTPWNTYTRDGL
                     PPTPIAMPGAASIAAAANPATTDYLYFVGRGDGSHQFSRTLEEHNRAVAKYQLGRS"
     gene            complement(7414..8238)
                     /gene="pabC"
                     /locus_tag="VFV55_07720"
     CDS             complement(7414..8238)
                     /gene="pabC"
                     /locus_tag="VFV55_07720"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEX4944228.1"
                     /translation="MALSLVNGLPARSVDSGDRGLMYGDGVFRTLVVRAGRALNWARH
                     FRLLAHDCGRLGLPCPAESLLLDEIGRVAPVEAIVKVIVTRGASGRGYACAADFAPTR
                     IVAAHPAPAPEIAEAARVGIRARRCDLALAIQPRLAGVKSLNRLENVLARAEWRDPAI
                     REGLLADSEGRLAEGTASNVFFTSRETLVTPDLSRCGVNGAQRERVLELARAAGIACQ
                     VRDAHFGELGEAGEVFLTNSVIGIWPVVALGEWRWAPGPVARRMQRLIEDDDARGA"
     gene            complement(8267..>9334)
                     /gene="fabF"
                     /locus_tag="VFV55_07725"
     CDS             complement(8267..>9334)
                     /gene="fabF"
                     /locus_tag="VFV55_07725"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX4944229.1"
                     /translation="FDPTQWMTPKEARRSDTFIHYGIAATKMALDDAGFVIDDANSER
                     TGVIVGSGIGGLPMIEDNILDMGTRGPRRISPFFVPGSIINMVAGLISIQFGAKGPNV
                     SMVSACTTSTHCVGEAGRLIEYGDADVMIAGGTEATICLSGMGGFCSLKALSERNDDP
                     QAASRPFDRDRDGFVLGEGAGILILEEYEHARKRGARIYCELAGMGMSADANHITAPD
                     MDGPRRAMLAAMRNGGINPDEVQYLNAHGTSTPLGDANETAAVKNAFGDHAGRLAVNS
                     TKSMLGHLLGAAGGVEALITVLAIHHQLSPPTINLDNADPACDLDYVPNTAREMRIDV
                     ALSNSFGFGGTNGTLAFRRLR"
BASE COUNT         1405 a         3307 c         3207 g         1415 t
ORIGIN      
        1 gggcctggtc tggcgcctac gcgatcttcg tggcggtgtg cgtctggctc gcctggcgca
       61 gccgcgggct cgagccgctc gcgccggtgg ccgcggctgc ggaggaggac accgagccgc
      121 ggccttcggc ccgctcgctc ctcgtgtggc tggtgctcgc cgcgatgggg tcggtgacgc
      181 tgctcgccgt ctccaaccac ctgacccaga acatctcctc gatcccgctg ctgtgggtga
      241 tcccgctcgc gatctacctc ctcaccttca tcctgtgctt cgaggggacg cgctggtacc
      301 ggcgcgacgc ctacctcggg ctcctcgtct ggatcgtgtg cgtgatggcc tggttcctgg
      361 ccgacaagcg cctgcagttc gagctcgcct ggcaagtcgc cgtgttcgcg gccggcctct
      421 acatcgtctg catgttctgc cacggcgagc tggcacgcct tcgcccggga ccgaagtacc
      481 tcacgcactt ctacctcgtc gtctcgctgg gcggcgtggt gggcggcatc ctcgtcgcca
      541 tcgtggcgcc gctcacgctc ccgggctacc tggaactcga gatcgcgctc gtcgtcgtgg
      601 cgctccttgc gctctacctc aatctcgacc gcccgaagcc cgtcgtcgcg atgttcgcgg
      661 ccgtggccct gttcaccgca ggcgcgcttg cctaccgcat cggcaacttc accgaggaca
      721 cggtgctgat cgagcgcaac ttctacggcg tcctgcgggt caaggagcag cgctcgcgcg
      781 acgacgaccc ggaaacgcgc taccgctcgc tggtgcacgg gtccatcctg cacggcgagc
      841 agtggatgag cgacaagtac cgtcgcgcgg cgacgaccta ctacaagaca tcgtccggca
      901 tcggcaaggc gatcctcgcc ttcgacggcc ggccgatcaa ggtgggcgtg atcggcctcg
      961 gcgcgggcac gctcgcgacc tacggcgatg ccgacgacac ctaccgcttc tacgaaatca
     1021 acccggccgt gccgcgcatc gcccgcgagc agttcacctt cctcggcgac agcccggccc
     1081 ggatcgagat cgtgctgggc gatgcgcgcc tgagccttga gcgcgagccc gtccagggat
     1141 tcgacgtgct cgccgtggac gccttctccg gcgattccat tccggtgcac ctcatcacgc
     1201 tggaggcggt gggtgcgttc ctgaggcaca tgaagcccac cggcgtcatc gccttccacg
     1261 tctcgaaccg gttcctcgac ctgaaaccgg tgctgctcgc gatcgcggag aggcatggcc
     1321 tcgaatacgc cttcgtccac gagacgggcg acgacggcgg caccacgagc gactgggtgc
     1381 ttctcacgcg caacaagccc ctcatcctgc gccccgaaat cgtttcggcg acggagcccg
     1441 tggcgccgca gccggactgg cgcctctgga cggacgccta caacaacctg ctccaggtct
     1501 ggcgccgcta gccgcgcagg caggccgcca cgcgcctcgc gacgatttcg ctcacgcgct
     1561 cgttcgattc ccgcgtgagg cccgcgatgt gcggcgtgag gatcaggttc ggcgcatcgg
     1621 ccagcacgct tcccgcgccg aggggctcgt ccgcgaagac atcgagcgcc gcccccgcca
     1681 gcctgccctg gtggagcgcc cgggcgagcg ccgcctcgtc gacgatgccg ccgcgcgcgg
     1741 aattcaccag gatcgcgtcc ggtttcatgc gcgcgaactg atcctccccg atcagcccgc
     1801 gcgtggaatc gacgagcggc aagtggagcg aaacgacgtc ggcgttcgcg agcaactcgt
     1861 ccagcccgca cggcccgacg ccgagttcgc tccacggagg ccaggcaacc ggcaaggcgg
     1921 gatcatgggc gatgacgcgc atgcccaggg cgatggcctt gcgggcggtc acgcggccga
     1981 tgcttccgaa gcccacgaga cccagcgtct ttcccgaaat ctcgcggccc tgggagagct
     2041 tctggcgcgg ccagccaccg gcggcgacgg cggcggtcga gagataggcg gcgccgcgca
     2101 acaggatcat cgccatcgcc aggacgtact ccgcgaccga ttcggcattg gcgccggtgg
     2161 ccgggatcac ctcgatgcca cgcgccgcgc aagcctcgag gtcgatgttg tccagcccca
     2221 cgccgagccg gcccacgacg cgcaggcggg aagctgccgc gagcgccgcg ccgcggacca
     2281 tcgtccggtt gcgcacgatc caggcgtcgg cctgcgcgag cgcgttcgac agccccgcca
     2341 cgtccagcgc gagcgccgga cggtagtcga cgtcgaaatc gcgcgcgaga aaggctaccg
     2401 cgggcgcgtc catgtcttcc gagatgacga tgcgctgcat gtttcgcttg ctccttgtct
     2461 attgcgaacc gcagatgaag gcgggatgaa agttgaaagc attggaacca cagatgaaca
     2521 cagatgaagc gccgcgctag cgcttccccg ccgcgagcca caccgtgatc tcctcgcgat
     2581 cgtggtagag atgcttcatg cgcaggcggt aggcaacgcc ggccagcgct tcctcgatga
     2641 tgccgcgcgc ccgcgccacc tcctcccagc gcttcttcat cggcagcttg aggttgaaga
     2701 tcgcctccct gcaccagcct tcggccaccc attgcgccgc cagcgtgcag acgcgcgacg
     2761 ggctctccac catgtcgcag accatccagt ccaccggctc cggcgggcgg tagcgaaatc
     2821 cgtcctcgcg ccggtgcttc acctggccgg actccatcag cgccgcgtcc atcgggccgt
     2881 tgtcgatggc cgtgaccatc aggccacgcc ggaccagctg ccaggtccac cccccgggcg
     2941 aggcgccgag atcgaccgcg gtcatgcccg gccgcatgcg cctggcgagg gcttcctcgc
     3001 cgatgaagag cgcgagcgcc tccgcgagct tgagcgtgga gcgcgacggc gccccgccgg
     3061 gcatgcgaat ccgcgcgatg cccatgggcc acgggcaact agcccccacg cgcgacgtgc
     3121 cgacgtagca cgcggcgccg ccaaggaaga acacgtgcag gcgcccggcg gccttcggat
     3181 cgtcgatggc gatgcccgct ttcggcagcg cgcgcagcag gtgcggttcg agcggcctga
     3241 tgagggaggc gagcgccttg ccgtcgttgg tgtcgggcgt ctcgaggaac gtctcgcgaa
     3301 aggaagtccc cagcgccgcg gcggcggaca cgatcggggc aacgcggtcg cccaccggaa
     3361 ggtcggcgag catttccccg cagcggacca cctggcgcgg aaagacgaga gcggtggcat
     3421 cgaggcgccg cgcgaacccg aggccggccg tcgcatcgtg cggatggaag accgcgaacg
     3481 cgctgtcggg cctcgccttc acgaagccct cgacgccggc ctcgcccgcc agtgccgtca
     3541 tctcctgcgc gcattccttc tcgaatcccg cccggcagta ggcgagcagg ttggcggcgc
     3601 tcatgccgcg cccgcgcccg cctcaggtcg ccggccgcgc atcgcggaag agggcgaaga
     3661 aattgcgcgt ggtcgcccgg gccacctcct cgaggctggt gcccttcagg cgcgcgattt
     3721 cctccgccac gtggctcacg aacgcgggct cgttggtctt gccgcggtgc ggcaccgggg
     3781 cgaggtacgg ggcgtccgtc tcgacgagca tgctctcgag cgggacatgc cgcgccacct
     3841 ccttgagcgc ctgggcattc ttgaaggtga cgatcccgga gaaggagatg aggaagccga
     3901 gtcgcatcgc ggcctgcgcg acctcgagcg attcggtgaa gcagtgcatg acgccgccca
     3961 cctcggacgc gcgctcctcc tcgaggatgc gcagggtatc ggcggccgcc tcgcgcgtat
     4021 ggatcaccag cggtttcgcg catgcccgcg ccgcgcgcac gtgcacgcgg aagcggctgc
     4081 gctgccattc ggtgtcgccc tcgacgcgga aatagtccag cccggtctcg ccgatcgcga
     4141 ccactttcgg atggctcgcc aagcgcacga gctcctcgac cgtcgcctcg cgcccgtcgc
     4201 gctgctcgtc ggggtgcacg cccgccgagc accacaggtt gggctgcgat tcggccacct
     4261 cgcgcacgcc cgggaagccc gccagcgtgg tcgagatggt gagcgcgtgc gtcaccccgc
     4321 gtgccgccat gcgggcgagg agcgccggca gctcggcgcg aagctcggga aagtcgagat
     4381 ggcaatgcga atcgacgagc atggcgcgca ttctaccggc gtgcgccgac cgccggcggg
     4441 cgcgtcagat cgtgtgcgtc tgccgcgtgg actgcaacgc cccggcgagc aggccttcga
     4501 tgcgcttctt gagctcgcgg caggtttcgg tgttgggcag ctggaccccg aacccctgcg
     4561 ggcggttgct cgcggcgtgc gcgcaattca cccaggccac gtgcccatga atcgggatct
     4621 tcacgctgtc gtcgagaagc gacaggagga tcagcacttc ctcgcccagg ttatgctcgc
     4681 gcgtgctcgg gacgaatatc ccgcccccgc gcagaagggg gatccatgcg gcatagagcg
     4741 ctgccttcga gcggatcacg agggagaaga ccgcgggccg cgtgatcgcg ggatcggttg
     4801 cgatgtcgtt catggctcag gaaagtgtcg cctgattgta tgccatcagc aagtgctccg
     4861 ctgcgagcct cgcgttcagc gggtgcgcga cgagccgccg cgcctcgagg agatcgcggt
     4921 ccagcgcaag cagacggtcg aggcgcgcgc ggcgggcctt ccccttgagt accgggacgc
     4981 aatcgacgtg atgccgcggc tcaccctggt tcttcaggcg cacgaggtcg tggacccagg
     5041 tctgcatcac gaagagcatg ggctcgagga gcacccggtc gaaacccgcc gcgaaggcga
     5101 gcacgtgtgc cccgcccgga cgcgacagct cggccacgat tttccggcgc cattcacgct
     5161 ccgcgggctg cgcgagttcg gcagccagca agggcgcgcc ccccgcgagc gcgagcgaga
     5221 cctccggttc gccggcaccg ccggccgtca gccacgcaag cgcttcctct cgcggtggtg
     5281 cacgcagcac gacggattcg cagcggctgc gaatggtcgc caggagccgg gccggacggt
     5341 cgctcaccag caccagcacc gtggccggcg gcggttcctc gagcgtcttg agcagcgcgt
     5401 tggccgcggc aggctgcagc gactcggcgg gatggagcac gatcgcacgg tggccggccc
     5461 ggtgggtgga aagcgaaacg aactcgcgga tcgcccgcac ctggtcaatc ttgatgacga
     5521 ggctcttctt gcccgcgtcc cggcccgcgt cggcttcctc gccctcgccc tcctcggcgg
     5581 attccggcac gatttcccgg tagtcgggat gattcgactg gccgaaccag tggcaggaag
     5641 ggcattgccc gcaggccagc cggtcgatgg gcgattcgca caggaggctg cgcgcgaatt
     5701 cgcgcgcgaa ttccaccttg ccgatcccgg ggcgccccga gacgaggagc gcatggggca
     5761 gccggtcgcg gcgccccatc aggcgctcca gaccctcgcg atgccagggc agtccgttca
     5821 cggaaaggcc ttcgcgaagt ccgaggcgag cctcgcgcgc acgtcctcga tcccgccgga
     5881 ggcgtcgatc acccgcaccc gcaggggatt ctcgcgtgct cgctcgaggt aggcgtcgcg
     5941 cacccgccgg aagaactccg tcgcctcgcg ctcgaacctg tccggcgtgc ggctctgggc
     6001 gcgctggcga tccatcgcga cggccggatc gaggtcgaac aggaacgtgg cgtccggatt
     6061 acagcgcgga tgcacgatcg attccagcgc cctgaagacg ccgcggtcga gcccgcgtcc
     6121 cccgcactgg taggcgaatg tcgcgtcgct gaagcggtcc gagagcaccc acgccccgcg
     6181 ggccagcgcc ggctcgatca cgcgaacgag gtgttcgcgc cgcgcggcga acatcagcag
     6241 ggcctcggtc gtgccttcca tcggctggtg caggacgagc tcgcgcagct tctcgcccag
     6301 cggcgtgccc ccgggttcgc gggtgacgac ggcctcgacg ccgcgcgcgc ggatcgccga
     6361 cgcgaggaac tcgaggtggg aggatttccc cgccccgtcg atgccctcca cggtgatgaa
     6421 acgaccggcc gtcatgatct tcccagctgg tattttgcca ccgcgcggtt gtgctcctcc
     6481 agggtgcgcg aaaactggtg cgagccgtcg ccgcggccga cgaagtacag gtagtcggtc
     6541 gtcgccgggt tcgccgccgc cgcgatcgag gccgcgccgg gcatcgcgat cggcgtgggc
     6601 ggcaggccgt cgcgggtata ggtgttccag ggcgtgtcgg cgaggaggtc gcgcttgcgc
     6661 aggtttccgt cgaagcgcgc gcccagcccg tagatcaccg cggggtcggt ctgcaggcgc
     6721 atgcccttgc gcaggcggtt cacgaacacc gaggcgatcg cgggcctctc gccggactgc
     6781 cccgtctcct tctcgatgat ggaggcgagg atcagcatct cgtaggcgct cgccaacggc
     6841 agcgccgggt cgcgcgactc ccaggcccgc gcgaggcgcc tttccatctc ggcatgcgcg
     6901 cgcttgagga tcacgacgtc ggtggttccc gaaaggaagt ggtacgtgtc ggggaagaag
     6961 agtccctcca gcgctgcgcc gggcagcccg atcgcggcgc gtatcccgtc ctcggacagg
     7021 ccctcgagcg tcgagcggac ctggggatgg ctgcgcatct gcgccagcca ctgccggaac
     7081 gtcgttccct cgacgaacac gatctcgatc ggcagcacgt cgcccgacgc cagcttgtcg
     7141 agcagctcga ggggcgtcag gggcgacgcg acgcgatagg ttccggcccg cagcgacccc
     7201 gccttgccga gaatccggcc gagcagccag aaggtctgcg cttcggggag aagtccttcc
     7261 tcggcgaggt tgcgggcaac ggtcttcagg ctcgctccgc tcttcacggt gaagtcgaaa
     7321 ggcacccgtg gcggctcgag gggccgcagg cagaaccagg ccgcccacgc cagggccgcg
     7381 atggcgaggc cggcagccga gaccaggacg agcctacgca ccgcgcgcat cgtcgtcctc
     7441 gatcaggcgc tgcatgcggc gggccacggg gcccggggcc cagcgccatt cgccgagcgc
     7501 gacaaccggc cagatgccga tgacgctgtt ggtcaggaac acctcgccgg cctcgcccag
     7561 ttcgccgaaa tgcgcgtcgc ggacctggca cgcgatcccc gcggccctcg cgagttcgag
     7621 aacgcgctcg cgctgcgctc cgttcacgcc gcagcgcgac aagtccggcg tgaccagcgt
     7681 ttcacgcgac gtgaagaaga cgttgctcgc cgtgccttcc gcgagtcgcc cttcggagtc
     7741 ggcgagcagt ccctcgcgga tggccgggtc gcgccattcc gctcgcgcca gcacgttttc
     7801 gaggcggttg agcgacttca cccccgcgag acgcggctgg atggcgagcg cgagatcgca
     7861 tcgccgtgcc cggatgccca cccgcgccgc ctcggcgatt tccggcgcag gcgcgggatg
     7921 ggccgcaacg atccgggtcg gcgcgaagtc cgccgcgcag gcatacccgc gccccgaggc
     7981 gccgcgggtc acgatcacct tcacgatcgc ttcgacggga gcaacgcgcc cgatctcgtc
     8041 gaggagcagc gattcggccg ggcatggcag cccgagccgg ccgcaatcgt gcgcaaggag
     8101 ccggaaatgg cgcgcccagt tcagcgcccg tcccgccctc acgacaagcg tgcggaacac
     8161 gccgtctccg tacatgaggc cgcgatcgcc cgaatcgacg gaccgggcgg gcaagccgtt
     8221 gacgagggac aaggccatgg agcgcgtggc cggcgcccgc tgctgcctag cgcaggcgcc
     8281 ggaacgcgag cgtcccgttc gtgccgccga agccgaagga attggaaagc gccacgtcga
     8341 tcctcatttc gcgcgccgtg ttcgggacgt agtcgaggtc gcaggcggga tcggcattgt
     8401 ccaggttgat cgtgggcggc gacaactggt ggtggatggc cagcaccgtg atgagcgcct
     8461 cgacgccgcc ggccgccccg agcaggtggc cgagcatcga cttggtggaa ttcaccgcga
     8521 ggcggccggc atggtcgccg aacgcgttct tcacggccgc ggtctcgttg gcgtcgccga
     8581 gcggcgtgga agtgccgtgg gcattcaggt actggacctc gtcgggattg atgccgccat
     8641 tgcgcatcgc ggcgagcatg gcgcggcgcg ggccgtccat gtcgggcgcc gtgatgtggt
     8701 tggcgtccgc gctcatgccc attccggcca gctcgcagta gatgcgcgcg ccccgcttcc
     8761 tggcgtgctc gtactcctcc aggatgagga tgcccgcgcc ctcccccagg acgaagccgt
     8821 cgcggtcgcg atcgaacggg cgcgaggccg cctgcgggtc gtcgtttcgt tccgacagcg
     8881 ccttgagcga gcagaacccg cccatgccgg acaggcagat cgtggcctcc gtcccgccgg
     8941 cgatcatgac gtcggcgtcg ccgtactcga tgaggcgtcc ggcctcgccc acgcaatgcg
     9001 tgctcgtcgt gcacgccgac accatcgaca cgttgggccc cttggcgccg aactgtatcg
     9061 agatgagccc cgcgaccatg ttgatgatgg agccggggac gaagaagggc gaaatgcgcc
     9121 gcggcccccg ggtgcccata tcgaggatgt tgtcctcgat catcggcagc ccgccgatgc
     9181 cggaccccac gatgacgccc gtgcgttccg aattcgcgtc gtcgatcacg aatccggcgt
     9241 cgtccagcgc catcttcgtg gccgcgatgc cgtagtggat gaaggtgtcg gagcggcgcg
     9301 cctccttggg cgtcatccac tgcgtgggat cgaa
//
LOCUS       DASUEV010000237         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1565717, whole genome shotgun sequence.
ACCESSION   DASUEV010000237 DASUEV010000000
VERSION     DASUEV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565717"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /gene="hslU"
                     /locus_tag="VFV55_07730"
     CDS             <1..901
                     /gene="hslU"
                     /locus_tag="VFV55_07730"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX4944230.1"
                     /translation="VPPSEVHEPGSTRQRFRKMLREGKLDDKEIEIDVAQGGGSMEIM
                     APPGMEELTQQIQGMFANLGGGRRRSRKLAIREALRLITDEEAGKLVNEEEIRLKAMR
                     AVEQNGIVFLDEIDKIASRGEMAGADVSRQGVQRDLLPLVEGTAVTTKYGMVRTDHIL
                     FIASGAFHLARPSDLIPELQGRLPIRVELSSLSVDDFERILTATDACLIRQYEALLAT
                     EDVALSFTPQAVRRIAEIAFSVNERTENIGARRLHTVMEKLLEEISFDAGRGVHEAKI
                     DAAYVDARLAELAKSEDLSRYVL"
     gene            976..1824
                     /locus_tag="VFV55_07735"
     CDS             976..1824
                     /locus_tag="VFV55_07735"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4944231.1"
                     /translation="MKRAFAAIAATLAGCTPMPQTADLRDAPASLAAAESAFAAQSVR
                     EGMRAAFLAWLAPDATIYRDGPVNGPSFVAAHADPPIILDWRPVFVEVAASGEMGLST
                     GPCKITSRKDPSAPPRHGQFVSLWKREPGGPWRVRVDLGISHPGPVLAEAPLQAGITP
                     PPGADRSTTLAEAEARFALRAARDGNAGAYAAAVSPTVRLYREGQAPFLGRESALASP
                     VAREGVTEWTVEAHEASASGDFGYATGRYGPPGGATAGHYLRVWRREPDGWRIALDVV
                     NARAPR"
BASE COUNT          351 a          757 c          734 g          335 t
ORIGIN      
        1 agtgccacca tccgaggtcc acgagcccgg ctccacgcgc cagcgcttcc gcaagatgct
       61 tcgcgagggg aagctcgacg acaaggaaat cgagatcgac gtcgcccagg gcggcggctc
      121 gatggagatc atggcgccgc cgggcatgga ggaactcacg cagcagatcc agggcatgtt
      181 cgcgaacctg ggcggcggcc ggcgcaggag ccgcaagctc gccatccgcg aggcgcttcg
      241 cctgatcacc gacgaggagg ccggcaagct cgtcaacgag gaggagatcc gcctcaaggc
      301 gatgcgcgcc gtcgagcaga acgggatcgt cttcctcgac gagatcgaca agatcgcgag
      361 ccgcggggag atggccggag ccgacgtctc gcgccagggc gtgcagcgcg acctgctgcc
      421 gctcgtcgag ggaacggcgg tcacgaccaa gtacggcatg gtgcgcaccg accacatcct
      481 gttcatcgcg agcggcgcct tccaccttgc caggccctcc gacctcattc ccgagctcca
      541 gggccggctg cccatccggg tggagctctc gagcctgtcg gtggacgatt tcgagcgcat
      601 cctgaccgcc accgacgcct gtctcatcag gcaatacgag gcgctgctcg ccaccgagga
      661 cgtggcgctt tccttcacgc cgcaggccgt gcgccgcatt gcggagatcg ccttttcggt
      721 gaacgagcgc acggagaaca tcggcgcgcg gcgcctgcac accgtgatgg aaaagctcct
      781 cgaggagatc tcgttcgacg ccggtcgcgg cgtgcacgag gcgaagatcg acgcggccta
      841 cgtcgacgcg cgactcgccg agctcgcgaa gagcgaggat ctgtcgcgct acgtgctcta
      901 gcgcaggaag gattggtcac ctgcgggcca cggccggtaa cggcgggcgc ggcggcccgg
      961 tccagaatcg ccaccatgaa acgcgccttc gccgccatcg cggccaccct tgccggctgc
     1021 acgccgatgc cccagaccgc cgacttgcgc gacgccccgg cgtcgctcgc cgccgccgag
     1081 tccgccttcg ccgcccagtc cgtccgcgaa ggcatgcgcg ccgcgttcct cgcctggctg
     1141 gcaccggatg cgacgatcta ccgcgacggg cccgtaaacg gcccctcctt cgtcgccgcg
     1201 catgcggacc cacccatcat cctcgactgg cggccggtct tcgtggaggt ggccgcatcc
     1261 ggcgagatgg ggctctccac cgggccgtgc aagatcacca gccgaaagga tccgtcggct
     1321 ccgccccgcc acggccagtt cgtttcgcta tggaagcgcg agcccggcgg cccctggcgc
     1381 gtgcgtgtcg acctcggcat ctcgcacccc ggcccggtcc tcgccgaggc accgctgcag
     1441 gcgggcatca cgccaccgcc cggcgctgat cgctccacca cgctggcgga ggccgaggca
     1501 cgcttcgcgt tgcgcgcggc ccgggacggc aacgcgggtg cctatgcggc ggcggtatcg
     1561 ccaaccgtgc gcctttatcg cgaagggcag gcaccgtttc tcggtcgcga atccgcgctg
     1621 gcgtccccgg tggcgcgcga aggagtcacc gaatggaccg tcgaagcgca cgaggcaagc
     1681 gcgtccggcg acttcggcta cgccacgggc cggtacggcc cgccgggcgg cgccacggcg
     1741 gggcactacc tgcgcgtctg gcggcgcgag cccgacggct ggcgcatcgc gctcgacgtc
     1801 gtgaacgccc gcgccccgcg ctaggccgcc agctacgaaa ctgcgccagg ccgccaaccg
     1861 caataccgca ctgctcgacg cttgaggatt ccttcgatcg agagcgatgc ggcctcggct
     1921 gggcacagtg gtgatcggag acggtgggtg gggtgaggtt ggatgtgtct tttttttgtt
     1981 ttgggccagg tcaaattgcg tcgggcgtca cggctaagac ctatgtcgag gtgtcgccga
     2041 agtcacggct ataagcaaat tcatctgggg cggattcaat cggtcaaagc aacacttcgt
     2101 ttagtttatc tgcgggagcc ttgaagccga gtgtctttcg cgggcgctgg ttcagttcta
     2161 gcgcaatcct gttcaga
//
LOCUS       DASUEV010000238         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_511705, whole genome shotgun sequence.
ACCESSION   DASUEV010000238 DASUEV010000000
VERSION     DASUEV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511705"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFV55_07740"
     CDS             <1..823
                     /locus_tag="VFV55_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX4944232.1"
                     /translation="KVRSSFAMTEPHPGSGSDPSMMLTTATRTGDKWVVRGRKWFITG
                     ADEAEHFIVVAKTSDDARKGLTAFLFHRDDPGWKVSRRIPILGPEEHGGHCELEFDAL
                     EIPDENRLMNVGDGLKVTQIRLGTARLTHCMRWLGLAKRCLDIAADYTGMRMSGGRKL
                     SERESVQAKLGHCAMQVEIGRLLTMKAAALLDKGDFARKEISMAKVHVADTLHLCADT
                     ALQLCGARGYSTDTPLEWIYRYARQARLVDGASEVHQQVLARFFLEERGDFWRWV"
     gene            814..1506
                     /locus_tag="VFV55_07745"
     CDS             814..1506
                     /locus_tag="VFV55_07745"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX4944233.1"
                     /translation="MGVTRGMPSDAEIGVLEALAADGQTIASAPGSCTPPFRARLRFV
                     DPGRGFLVLESSGDKAADAAFLARPRTELLVEWGEWRIAFAGEEPHAASHEGVAAIRI
                     RFPESVSISRRRMFERAPVPERAPLHCVAYSGASVIMDATITDMSQGGIGLQVESAGD
                     ALQPGMVLLGCRIESPGREPVVVHLEVRHTATTRLPDGSHTVRAGCRFTNLSPRATAF
                     VTEYLSATAQGV"
     gene            1617..2951
                     /locus_tag="VFV55_07750"
     CDS             1617..2951
                     /locus_tag="VFV55_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEX4944234.1"
                     /translation="MIPRPVSPRSPSAALLAASLCAAILATLPVRADETCNSPYMGNL
                     IKGQEDFVYVWTLGVEGLGDGLDKLVTIDVNPKSKSYGKVVASVSVDARGEAHHMGFT
                     DDRRFLWAGGLASNKIHVFDVATQPAKPKLVRTIANLGDKAGWLGPHTFYAMPGRMLV
                     GSLSNTKDKGGTTGMAMYNNQGEFVSSHAMPTDMGGDGYGYDIAINPARNALLTSSFT
                     GYDNYMRNLGEVVKDAEAMKRFGNTMVMWDLKAMKPKQVLKVPGAPLEIRWSLKPGDN
                     WAITAAALTSKLWLVKQAKGGQWEAKEVGTIGDPAKIPLPVDISITADGKGLWVNTFM
                     DGKTRYFDLTNPEQPRQTYEKATGRQVNMISQSWDGKRVYITSSLLANWDKAGADNEQ
                     FLRGFRWDGKELALAFEVDFAREKLGRAHHMKFGTAKLRSALGEVPEQLAVR"
     gene            2957..>3460
                     /locus_tag="VFV55_07755"
     CDS             2957..>3460
                     /locus_tag="VFV55_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX4944235.1"
                     /translation="MRFRAALAGVFFALALAGPAAAHVDEARDPPAFDPPSPGSYRLY
                     RIQRAPDGEVLDSSGNARPLSAFTHGRITILGLVYTRCADPDGCPRSTWAFQEVRDLL
                     RAERSLEKRVRLVSLSFDPAHDTPELLRSWGAGARGRAKGADWHFLTTRSTRALVPIL
                     DGFGQDLR"
BASE COUNT          600 a         1180 c         1157 g          523 t
ORIGIN      
        1 gaaggtgcgc tcctcgttcg ccatgaccga accgcatccc ggcagcggtt ccgacccttc
       61 catgatgctc acgaccgcaa cccggaccgg cgacaagtgg gtggtgcgcg ggcgcaaatg
      121 gttcatcacc ggcgcggatg aagcggagca cttcatcgtc gtcgccaaga catcggacga
      181 cgcgagaaaa ggcctcacgg cattcctgtt ccaccgcgac gaccccggct ggaaggtttc
      241 gcggcgcatc cccatcctcg gccccgagga gcacggcggg cactgcgaac tggaattcga
      301 cgccctcgag attcccgacg agaaccggct gatgaacgtg ggcgacgggc tgaaggtcac
      361 gcagatccgc ctcggcacgg cgcgcctcac gcactgcatg cgctggctgg ggcttgcgaa
      421 gcgttgcctc gacattgccg cggactacac cggaatgcgc atgagcggcg ggaggaagct
      481 ctccgagcgg gagagcgtgc aggcgaagct gggccactgc gcgatgcagg tcgagatcgg
      541 gcggctcctc accatgaagg ccgcagcact tctcgacaag ggcgacttcg cgaggaagga
      601 gatctccatg gccaaggtgc acgtagccga cacgctgcac ttgtgcgccg atacggcact
      661 tcagttgtgc ggcgcccgtg gctactcgac cgacacgccg ctcgagtgga tctaccgcta
      721 cgcgcggcag gcgcgcctgg tggacggcgc ttccgaggtc caccagcagg tgctggcgcg
      781 attcttcctc gaggaacgcg gggatttctg gagatgggtg tgacacgggg catgccgtcg
      841 gacgccgaga tcggcgtcct cgaggcactg gccgcggatg gccagactat cgcctcggcg
      901 ccaggctcct gcacgccgcc gttccgcgca cgcctgcgct tcgtcgatcc cgggcgcgga
      961 ttcctcgtcc tggaaagcag cggcgacaag gcggccgatg cggcctttct cgcccggccg
     1021 cgcacggaat tgctcgtgga gtggggcgag tggcgcatcg cctttgcggg cgaggagccg
     1081 cacgccgcgt cgcacgaggg cgtggccgcg atacgcatcc ggtttcccga atcggtgtcg
     1141 atcagccggc ggcgaatgtt cgagcgcgcc ccggtgccgg agcgggcgcc gcttcattgc
     1201 gtggcctatt cgggcgcctc ggtcatcatg gacgccacca tcaccgacat gagccagggc
     1261 ggcatcggcc tccaggtcga atcggccggc gatgcgctgc aaccgggaat ggtcctcctc
     1321 ggctgccgca tcgaaagccc gggacgcgag ccggtggttg tccacctcga ggttcgccat
     1381 acggccacga cccggcttcc cgacggcagc cacacggtac gtgccggttg ccggttcacg
     1441 aacctgtcgc cccgggcaac ggctttcgtg accgagtatc tgtccgcgac ggctcagggc
     1501 gtctagcagg gcgccgcgcg cgcattgacc gcccatgcgg ttgcgggggt aggatggcgc
     1561 cgccgcacca tgacacccgc aggcaggacc gaaacgaacc gactcaggag gccgcgatga
     1621 tccctcgtcc cgtttccccc cgatcgccgt cggcggcgct actcgccgcc agcctttgcg
     1681 cagcaatcct tgccactctc cccgtccgtg ccgacgaaac ctgcaactcg ccgtacatgg
     1741 gcaacctcat caaggggcag gaggacttcg tctacgtctg gaccctcggc gtcgagggcc
     1801 tgggcgacgg cctcgacaag ctcgtgacca tcgacgtgaa cccgaaatcg aagtcctacg
     1861 gcaaggtcgt cgcctcggtc tccgtcgatg cgcggggaga ggcgcatcac atggggttca
     1921 ccgacgatcg ccgcttcctg tgggccggcg ggctggccag caacaagatc cacgttttcg
     1981 acgtggccac gcagccggcg aagccgaagc tggtccgcac catcgcgaac ctgggcgaca
     2041 aggcgggctg gctcggcccg cacaccttct acgccatgcc cggacggatg ctggtcggca
     2101 gcctgtcgaa caccaaagac aagggcggca ccacgggcat ggcgatgtac aacaaccagg
     2161 gagaattcgt ttcctcccac gcgatgccca ccgacatggg cggcgacggc tacggctacg
     2221 acatcgccat caatccggca aggaacgcgc tcctcacgtc gagcttcacc ggctacgaca
     2281 actacatgcg caacctcggc gaggtcgtga aggatgccga ggccatgaag cgctttggca
     2341 acacgatggt gatgtgggac ctcaaggcga tgaagccgaa gcaggtgctc aaggtcccgg
     2401 gcgcgccgct cgagatccgc tggtcgctca agcccggcga caactgggcc atcaccgccg
     2461 ccgcgctcac ctcgaagctc tggctggtga agcaggccaa gggcggccag tgggaggcga
     2521 aggaggtggg caccatcggc gacccggcga agatcccgct gccggtcgac atcagcatca
     2581 ccgccgacgg caagggcctg tgggtcaaca ccttcatgga cggcaagacg cgctacttcg
     2641 acctcacgaa cccagagcag ccgaggcaga cctacgagaa ggccaccggc aggcaggtga
     2701 acatgatttc gcagagctgg gatggcaagc gcgtctacat cacctcgtcg ctcctcgcca
     2761 actgggacaa ggcaggggcc gacaacgagc agttcctgcg cggcttccgc tgggacggca
     2821 aggaactcgc gctggccttc gaggtggatt tcgccaggga gaagctcggc cgcgcccacc
     2881 acatgaagtt cggcacggcc aagctcaggt cggcactggg cgaggtaccg gagcagctcg
     2941 cggtccgctg accgggatgc gctttcgcgc tgcgctcgcc ggcgtcttct tcgccctggc
     3001 gctggcgggc ccggcggcag cgcacgtgga cgaggcccgc gacccgcccg cattcgatcc
     3061 gccttcgccc ggaagctatc gcctctaccg catccagcgg gcgccggacg gcgaggttct
     3121 cgattcgagc gggaacgcgc ggccgctttc ggcattcacg cacgggcgca tcacgatcct
     3181 cgggctcgtc tatacgcgtt gcgcggaccc ggacggatgc ccgagatcga cctgggcgtt
     3241 ccaggaagtt cgcgacctgt tgcgcgccga gcgttcgctc gagaaacgcg ttcgcctggt
     3301 gagcctgtcg ttcgaccccg cccacgacac gcccgagctc ctgcgcagtt ggggcgcggg
     3361 cgcccgggga cgcgcgaagg gcgccgactg gcatttcctc accacgagat cgacgcgggc
     3421 gctcgtcccg atactcgacg gcttcgggca ggaccttcgc
//
LOCUS       DASUEV010000239         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_875013, whole genome shotgun sequence.
ACCESSION   DASUEV010000239 DASUEV010000000
VERSION     DASUEV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875013"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VFV55_07760"
     CDS             complement(<1..1195)
                     /locus_tag="VFV55_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX4944236.1"
                     /translation="MSARPLKIGKYNVLSEIGQGASALVYVGEDPFNDRKVAIKVAKS
                     DADMSEEEANRFEKLFLNEASLAGKLNHPNIVAVFDAVVDGDQRYIVMEYVPGGSLKK
                     YCTETNLLPVRQAVLVVFKMCRALDYAFQNGVIHRDIKPANILLSERDDIKISDFGTA
                     RISHATHTQIEGFLGSPAYMSPEQINEESPSVQTDIYSLGVVMYELLTGKLPFRAENS
                     VAMINKILNEEPMPLEQLRPDLPAKLVEIVKKAMAKDPEERYQTWFEMASDLADTFPQ
                     LERYSNEISSTEKFNKLRALPFFREFRDAELWEVLRGAIWEVHARDENLLLEGEIGQA
                     FFIIVSGQVKVVKDGKLLNVLKEGDCFGEMAYLSGDRARRSASITSVSEVHLLKIQAA
                     HLESLT"
     gene            1366..1926
                     /locus_tag="VFV55_07765"
     CDS             1366..1926
                     /locus_tag="VFV55_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX4944237.1"
                     /translation="MQESLFEAVRTGDVEGAGRLAVGDALLATDAQGTTALMLAARLG
                     NSRMVAALLAAGADVNAADALGFTPLFHACYNLDEDCGYPGVVQLLLDAGADKEAKIG
                     YGVRPLMYAAGNGEAGVVEVLLRAGADPAARNEGGRTALMMVKDRDYVDVINLLHEAE
                     LASGGEGCGTRNAPGMQVVTFLKREK"
BASE COUNT          339 a          628 c          706 g          375 t
ORIGIN      
        1 cggtgaggct ctccaggtgc gcagcctgga tcttgagcag gtgcacctcc gacacggagg
       61 tgatcgatgc cgagcggcgc gcgcggtccc cggagaggta ggccatctcg ccgaagcagt
      121 cgccttcctt gaggacgttc agcagcttgc cgtccttcac cactttcacc tgccccgaga
      181 cgatgatgaa gaacgcctgc ccgatctcgc cctccagcag caggttctcg tcgcgcgcgt
      241 gcacctccca gatggcgccg cgcagcactt cccacagctc cgcgtcgcgg aactcgcgga
      301 agaagggaag ggcgcgcagc ttgttgaact tttccgtcga gctgatctcg ttggagtagc
      361 gctcgagctg cgggaaggtg tcggcgaggt cgctcgccat ctcgaaccag gtctggtagc
      421 gctcctcggg atccttcgcc atcgccttct tcacgatctc caccagtttc gcggggaggt
      481 ccgggcgaag ctgctcgagg ggcatgggct cctcgttcag gatcttgttg atcatcgcga
      541 ccgagttctc ggcgcggaag gggagcttgc cggtgaggag ctcgtacatg accacgccta
      601 gggagtagat gtccgtctgg accgacggcg actcctcgtt gatctgctcg ggcgacatgt
      661 aggcgggcga gcccaggaag ccctcgatct gggtatgcgt cgcatgcgag atcctggcgg
      721 tgccgaagtc gctgatcttg atgtcgtcgc gttccgacag caggatgttg gcgggcttga
      781 tgtcgcggtg aatcacgccg ttctggaacg cgtagtcgag cgcgcggcac atcttgaaga
      841 ccaccagcac cgcctggcgc accggcagca ggttggtctc cgtgcagtac ttcttgagcg
      901 accccccggg gacgtactcc atcacgatgt agcgctggtc gccgtcgacg accgcgtcga
      961 acaccgcgac gatgttggga tggttcagct ttccggcgag cgaagcctcg ttgaggaaga
     1021 gcttctcgaa ccggttggct tcctcctccg acatgtcggc gtcgcttttc gcgaccttga
     1081 tcgcgacctt gcggtcgttg aacgggtcct cgccgacata gaccagggcg gaggccccct
     1141 gcccgatctc ggacaggacg ttgtacttgc cgatcttgag ggggcgcgcg gacaccggga
     1201 tgggcaggag gggaagcggg gagaatgcgt cttcggggga gtgtaaccga actgccggcg
     1261 catcgcgaaa ggtccctggg gcgagcaccg gctgcttttt gcgttgatcc ccgcgcccgc
     1321 gcccgcgata atggacgatt cattgactgg atcggggatg ccggcatgca ggaaagtctg
     1381 ttcgaggcgg tgcgcacggg agatgtcgag ggggccgggc ggctcgcggt cggcgatgcg
     1441 ctgctcgcca ccgacgcgca gggcacgacc gcgctcatgc tggccgcgcg cctgggcaat
     1501 tcgaggatgg tggccgcgct gctcgcggcc ggcgccgacg tcaatgccgc cgacgcgctc
     1561 ggattcacgc cgctttttca cgcctgctac aacttggacg aggactgcgg ctaccccggc
     1621 gtggtccagc tccttctcga tgcaggcgcc gacaaggagg cgaagatcgg ctacggcgtt
     1681 cgccccctca tgtatgccgc cggcaatggc gaggcgggcg tggtggaggt cctgcttcgc
     1741 gccggcgccg accctgctgc caggaacgag ggcggccgca cggcgctgat gatggtcaag
     1801 gaccgcgatt acgtggacgt gatcaacctc ctgcacgagg cggaactggc gagcggcggc
     1861 gagggttgcg gaacgcgaaa cgccccgggg atgcaggtcg tcaccttcct caagcgagag
     1921 aagtaggcat ttcaccgaat gccggggatt cgatgaaggt ccgaaacagg gccgggggct
     1981 agccaccatc attcgccagt cggcagcgaa caggatgaat cccgtcgggt tggttccgaa
     2041 ttgagctt
//
LOCUS       DASUEV010000240         3912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1202161, whole genome shotgun sequence.
ACCESSION   DASUEV010000240 DASUEV010000000
VERSION     DASUEV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202161"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFV55_07770"
     CDS             complement(<1..842)
                     /locus_tag="VFV55_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944238.1"
                     /translation="MSKFTRREFLKTTAGAAAAGSLGVGSAVWTQDAYAQAKWTPEKG
                     AKLRVLRWKRFVAGDEDTWMINVKKFTQKTGVEVRVDNEGWEDVRPKAAVAANVGSGP
                     DIIVGWFDDPHQYPDKLVDVTDVANYLGGKYGGWYDVCRKYGTRNNKWIAIPLGIIGN
                     AIVYRESHVKAAGFDGIPKDTAGFLKLMQALKAKGTPGGFALGKAVGDGNNWAHWLLW
                     SHGGKMVDDKGNVVINSPETIASLEYAKQLYETFVPGTLSWLDPSNNKAFLDGQVSLT
                     GNGIS"
     gene            complement(899..1978)
                     /gene="ugpC"
                     /locus_tag="VFV55_07775"
     CDS             complement(899..1978)
                     /gene="ugpC"
                     /locus_tag="VFV55_07775"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX4944239.1"
                     /translation="MASVTIRNVEKFFGGTHVIRGVDIQIADGEFAVLVGPSGCGKST
                     LLRMIAGLEEISGGEIAIGDKVVNDVQPKERDIAMVFQNYALYPHMKVRDNMAFSLLL
                     AKRPRSEIDERVNKAAGILGLTDLLERFPRQLSGGQRQRVAMGRAIVRNPQVFLFDEP
                     LSNLDAKLRVAMRTEIKELHQRLKTTSVYVTHDQIEAMTMADQIVVMKDGLVEQRGRP
                     LDLYDHPSNLFVAGFIGSPAMNFLPGAVTGSGGEARIALDGGGSVPAPRGSAGTDGQR
                     IVYGVRPEHLVLGGDRDGIAAKVVVVEPTGMDTQVFGKYGDVELTAVFRERHEFTAGE
                     SIYLVPDHARTHLFDAGSGKSLMQR"
     gene            complement(1979..2950)
                     /locus_tag="VFV55_07780"
     CDS             complement(1979..2950)
                     /locus_tag="VFV55_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX4944240.1"
                     /translation="MARFLITDYDFPDVELELALYRDAGMEVKTAQCRTVDEVIEASQ
                     GCDGLLVQYAPVDAKVFAARPGIRIASRYGAGFDTIDTEDAKRHGVWVANSPDYGVGE
                     VATHALGMALCLLRHLAIYDRDVKSGRWHYTTPGTIRRASDMTLGILGLGRIGKRMAH
                     VSRNCFRRVIACDPYIIDGDFPAYVGRVSLDELFGQSDVVSLHVPLTAETRGMVEAKL
                     LGLMKPGSMLVNTARGAVVNVEDMAAALETGRLDGAALDVLPHEPPPAGHPLLAHPRV
                     LLTPHAAFYSVEAERELRRKAAQNLIDWARTGRPRYVVVEGRNASGS"
     gene            complement(2950..>3912)
                     /locus_tag="VFV55_07785"
     CDS             complement(2950..>3912)
                     /locus_tag="VFV55_07785"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX4944241.1"
                     /translation="REAFENAIRVNAAIGGSTNAVIHLIAIARRIGVKLTLEDWDKFG
                     RGVHTLVNLMPNGKYLMEDFCYAGGLPVVIRELGKQGLLNKKALTANGETIWANNRKA
                     ENFGPDVIMPFAKPFKRDTGIAVLRGNLCPDGAIIKPSAATPKLMKHTGRAVVFEDIE
                     DFNARIDSPKLAIDADSVMVLKNCGPKGYPGMAEVGNMPLPAKLLKKGVTDMVRISDA
                     RMSGTAYGTVVLHAAPEAAAGGPLALVKDGDLIELDVAQRKLHVHVSDEELARRRTAW
                     KKPKPPMSRGYWKLYVDHVNQASEGADLDFLVGKSGSDVPRDNH"
BASE COUNT          584 a         1350 c         1264 g          714 t
ORIGIN      
        1 gagatgccgt tgccggtgag cgagacctgc ccgtcgagga aggccttgtt gttcgacgga
       61 tcgagccagg agagcgtgcc cggcacgaag gtctcgtaga gctgcttcgc gtactcgagc
      121 gaggcgatgg tctccggcga gttgatgacg acattgccct tgtcgtccac catcttgccg
      181 ccgtgcgacc agagcagcca gtgcgcccag ttgttgccgt cgcccaccgc cttgcccagc
      241 gcgaagccac cgggcgtgcc cttggccttg agcgcctgca tgagcttgag gaatccggcc
      301 gtgtccttgg ggatgccgtc gaagcccgcg gccttcacgt ggctttcgcg atagacgatc
      361 gcgttgccga tgatgcccag cggaatggcg atccacttgt tgttcctcgt gccgtacttg
      421 cggcacacgt cgtaccagcc gccgtacttg ccgcccaggt agttggcgac gtcggtaacg
      481 tccaccagct tgtcggggta ctggtgcggg tcgtcgaacc agccgacgat gatgtccggg
      541 ccgctgccca cgttggccgc caccgccgcc ttgggccgca cgtcctccca gccctcgttg
      601 tccacgcgca cctcgacacc cgtcttctgc gtgaatttct tcacgttgat catccaggtg
      661 tcctcgtctc ccgccacgaa gcgcttccag cgcaacacgc gcagcttcgc gcctttctcc
      721 ggcgtccact tggcctgcgc gtacgcgtcc tgcgtccaga ccgcgctgcc cacgcccagc
      781 gagccggccg ccgccgcgcc cgccgtcgtc ttcaggaatt cccgtctcgt gaacttcgac
      841 attgcaatct cctctttctt gttggcacac catccgggcc gctgcccgac cgttccgact
      901 acctctgcat caaagacttc cctgatccgg cgtcgaacaa atgcgtgcgg gcgtggtccg
      961 gaaccaggta gatcgactcg cccgccgtga actcgtggcg ctcgcggaag actgcggtca
     1021 gttccacgtc gccgtacttg ccgaagacct gcgtatccat cccggtgggc tccaccacca
     1081 ccaccttcgc ggcgatgccg tcgcggtcgc cgccgagcac gaggtgttcc gggcgcacgc
     1141 cgtagacgat gcgctgccca tccgttccgg cgcttcccct gggcgcgggc accgagccgc
     1201 cgccgtcgag cgcgatgcgc gcctcgccgc ccgaaccggt caccgcgccg ggcaggaaat
     1261 tcatggcggg cgagccgatg aagcccgcga cgaagaggtt cgagggatgg tcgtagagat
     1321 cgagcggcct gccccgctgc tcgacgaggc cgtccttcat caccacgatc tggtccgcca
     1381 tggtcatcgc ctcgatctgg tcgtgcgtca cgtagaccga ggtggtcttc aggcgctggt
     1441 gcagttcctt gatctcggtg cgcatcgcca cgcgcagctt cgcgtcgagg ttcgacagcg
     1501 gctcgtcgaa gaggaacacc tgcgggttgc gcacgatggc gcgtcccatg gccacgcgct
     1561 ggcgctggcc gccggagagc tggcgtggga agcgctcgag caggtccgtg agcccgagga
     1621 tgcccgccgc cttgttcacg cgctcgtcga tctccgagcg cgggcgcttg gcgagcagca
     1681 ggctgaaggc catgttgtcg cgcaccttca tgtgcgggta gagcgcgtag ttctggaaca
     1741 ccatcgcgat gtcgcgttcc ttgggctgga cgtcgttcac caccttgtcg ccgatggcga
     1801 tctcgccgcc ggagatctcc tccagccccg cgatcatgcg cagcagcgtc gacttgccgc
     1861 agcccgaagg ccccaccaga accgcgaact ccccgtccgc gatctggata tcgacgccgc
     1921 ggatgacgtg cgtgcccccg aagaatttct cgacgttgcg gatggttacg gatgccattc
     1981 aggaccctga agcgttgcgg ccttcgacca ccacgtagcg cggccggccg gtgcgcgccc
     2041 aatcgatgag attctgcgcc gctttgcggc gaagttcacg ttccgcctcg acggagtaga
     2101 acgccgcgtg cggcgtgagc aggacgcgcg gatgggcgag gagcggatgc ccggccgggg
     2161 gaggctcgtg cggcaggacg tcgagcgccg cgccatcgag ccttcccgtt tccagcgccg
     2221 ccgccatgtc ctccacattc accacggcgc cacgcgccgt gttcaccagc atggaacccg
     2281 gtttcatcag cccgagcagc ttcgcctcca ccattccccg tgtctcggcg gtgaggggaa
     2341 cgtgcagcga caccacgtcg gactgcccga acagttcgtc cagcgatacg cgccccacgt
     2401 aggccggaaa atcgccgtcg atgatgtagg gatcgcaggc gatcacgcgc cggaagcaat
     2461 tgcggctcac gtgcgccatg cgcttgccga ttcgcccgag gccgaggatg ccgagcgtca
     2521 tgtcggaggc gcgccggatc gtgccgggcg tcgtgtagtg ccagcggccg gatttcacgt
     2581 cccggtcgta gatcgcaagg tggcgaagaa ggcagagcgc catgccgagg gcgtgcgtgg
     2641 ccacctcccc cacgccgtag tcgggagaat tcgcgaccca caccccatgg cgcttcgcat
     2701 cctcggtatc gatggtgtcg aagccggcgc cgtagcgcga ggcgatgcgg ataccggggc
     2761 gcgccgcgaa caccttggcg tccaccgggg cgtactgcac gagaaggccg tcgcagccct
     2821 gcgacgcctc gatcacctcg tccacggttc ggcactgcgc cgtcttcact tccattcccg
     2881 cgtcgcgata gagcgcgagc tcgagctcga cgtcgggaaa gtcgtagtcg gtaatgagga
     2941 aacgcgccat cagtggttgt cccgcggcac gtccgagccg ctctttccca cgaggaaatc
     3001 gaggtcggcg ccctcgctcg cctggttcac gtggtcgacg taaagcttcc agtagccacg
     3061 gctcatcggg ggtttcggct tcttccaggc ggtccggcgc ctcgcgagtt cctcgtcgct
     3121 cacgtgcacg tggagcttgc gctgcgcgac gtcgagctcg atcaggtcgc cgtccttcac
     3181 cagcgcgagc gggccgccgg cagccgcctc cggcgcggca tgcaggacca cggtgccgta
     3241 cgcggtgccg ctcatgcgcg cgtccgagat gcgcaccatg tcggtcacgc ccttcttcag
     3301 cagtttcgcg ggcagcggca tgttgcccac ctcggccatg cccgggtagc ccttcgggcc
     3361 gcagttcttg agcaccatga ccgaatcggc gtcgatcgcg agcttcgggc tgtcgatccg
     3421 ggcgttgaag tcctcgatgt cctcgaagac gacggcgcgg cccgtgtgct tcatgagctt
     3481 cggggtcgcc gcggagggct tgatgatcgc gccgtcgggg cagagattgc cgcgcagcac
     3541 cgcgatgccc gtgtccctct tgaacggctt cgcgaagggc atgatcacgt cgggaccgaa
     3601 attctcggcc ttcctgttgt tggcccagat cgtctcgccg ttggcggtga gcgccttctt
     3661 gttgaggagg ccctgcttgc ccagttcacg gatcaccacc ggcagcccgc cggcgtagca
     3721 gaagtcctcc atcaggtact tgccgttggg catcaggttc accagcgtgt gcacgccgcg
     3781 gccgaacttg tcccagtcct cgagcgtgag cttcacgccg atgcggcgcg cgatggcgat
     3841 caggtggatc accgcgttgg tcgagccgcc gatggccgcg ttcacgcgga tggcgttctc
     3901 gaaggcctcg cg
//
LOCUS       DASUEV010000241         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_170297, whole genome shotgun sequence.
ACCESSION   DASUEV010000241 DASUEV010000000
VERSION     DASUEV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170297"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..885
                     /gene="prmA"
                     /locus_tag="VFV55_07790"
     CDS             4..885
                     /gene="prmA"
                     /locus_tag="VFV55_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX4944242.1"
                     /translation="MSVVVPGRSAGALADALEAAGAVSTELADADAGTAAESAIFAEP
                     GADAGVWPHCRLTALLPSHANARAVLEAALAQVGCRPLEPPALDELEDTDWVRESQRQ
                     FSPIRAGERLWIVPTWHEAPDASAVNIVLDPGAAFGTGSHPTTRLVLGWLEREVHGGE
                     TVLDYGCGSGILAIAAMKLGAGRAVGVDIDPLALEAARYNASANAVDLDVRAADADLQ
                     LEADLVVANILANPLRMLAPLLASRTRPGGRIALSGILVAQSGEVMQAYAPFFDMAPA
                     GIEGDWVCLSGTRRQGA"
     gene            888..1925
                     /locus_tag="VFV55_07795"
     CDS             888..1925
                     /locus_tag="VFV55_07795"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEX4944243.1"
                     /translation="MLVTACRHCGARFRVTPEQLNLRQGQVRCGQCHQVFNGFESLER
                     FPGDDTGARLLAARAANGTHPDPVAELDDADLGRPVEHAVPSGQPPFLDSLPQEASPV
                     PVGPPDSSGLIDVPDLLPESSVSKEVLLEAPSEGRSGGPPEMPSEALPEAPYKTAARV
                     PPVVVTAQSVRRAQLARDGAFVADPAARPSRAWAFGVALLALVLALQLAYAFRAPIAQ
                     QYPVLRPILESACAAAGCSVPWVNDEAALKLEDSELLEVPGKPGQIALQARIRNLGTS
                     AQEFPHLELTLTDATGQTAVRRVLRPADYLGRVPAAGEVMAAGSEALLSVRLETGRIR
                     PTGYELLLFYP"
     gene            complement(1916..>4106)
                     /locus_tag="VFV55_07800"
     CDS             complement(1916..>4106)
                     /locus_tag="VFV55_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HEX4944244.1"
                     /translation="SLDAHARARARLSPHESAGVLTDCRDLALGRIVKALAGVLDRVE
                     DELFQLAESSRDRETQNLYLDARTQARGRRPVIEAAFRRQFLEFFNRKVKGEPAAADA
                     GAPPRALALLDDDELEHMLAVQGMAARLKAACEGELAMLSQRFGYLLEKPDLADEANP
                     MSPETICGALKEACDQIEAGWKVRLTLLRVLEGQVAGELRRVYRDLNVHLAERQILPE
                     IRPSYRRSPASPATGDDRQPGAPATTGAGPAPDVHAALARLLLAASAPADALSALPEE
                     PAVSPATRGFVKLLTSLQLPGSGANSVATGLPNVIRELRESPQSSILVGGDAMTIDIV
                     AMLFDFVFEDRQIPVAAKALLGRLQIPTLKVALLDRTFFSTRTHPARQLLDRLARAAI
                     GLDESSARGADTLAKLEVVVGRVLDEFDDDLAVFATLAADMESFLARQDQAEEAIALR
                     TARLVEGRERLEMARVLAEDEVDRRIAAREWVPEAVRAMLEDIWVRALATVHVEQGEG
                     CAQWAAMLATVDELLWSVERKSRTEDRRRLVARIPALVRSLQEGMSRAGVADAISESF
                     LGELVDCHADAVRAGLKGTVLAGQPTVPEPGQPRAFVAPALERALVPAGDVHIEEIRL
                     RSPRGQTPVPGAYARSGIWTNVKRHTWVEFRREVAQPIRARLTWISPARGVYLFTNAN
                     ASAAISVSPEALAELMRRGEARIVDDAPLVDRVVDSMLASLREGVQG"
BASE COUNT          588 a         1508 c         1409 g          601 t
ORIGIN      
        1 cgcttgagcg tggtggtgcc cggacgcagc gcaggcgcgc tggcggacgc cctggaggct
       61 gccggcgccg tttccaccga actcgccgac gcagacgccg gcaccgccgc cgaaagcgcg
      121 atcttcgccg agccgggcgc cgacgcgggc gtttggccgc actgccgcct cacggcgctc
      181 ctgccctccc atgcgaatgc cagggccgta ctcgaggcgg cgctggcgca agtcggttgc
      241 aggccgctcg agccgcccgc cctcgatgaa ctcgaggaca ccgactgggt gcgcgagtcg
      301 cagcgccagt tctcgcccat ccgcgcggga gagcggttgt ggatcgtgcc gacatggcac
      361 gaggcgcccg atgcgagcgc ggtcaacatc gtgctcgacc cgggcgcggc tttcggcacg
      421 ggcagccacc ccaccacgcg gctcgtcctc ggctggctcg aacgcgaggt gcacggcggc
      481 gaaacggtgc tcgactacgg ctgcggctcg ggcatcctcg ccatcgccgc gatgaagctg
      541 ggcgcggggc gcgccgtggg cgtcgacatc gatccgctcg ccctcgaagc cgcccgctat
      601 aatgcttcgg ccaacgcggt cgacctcgac gtgcgcgccg ccgacgccga cctccagctg
      661 gaagccgacc tcgtcgtcgc caacatcctc gccaatccgc tgcgcatgct ggcaccactc
      721 ctcgcttcgc gcacgcgtcc cggggggcgc atcgcgctct cgggcatcct cgttgcgcaa
      781 tcgggcgaag tgatgcaggc ctatgccccg ttcttcgaca tggcgcctgc cggcatcgag
      841 ggcgactggg tgtgcctttc gggcacgcgc aggcaagggg catagcgatg ctcgtcaccg
      901 cgtgcaggca ctgcggcgcg cgcttccgcg taacacccga gcagctgaac ctccgccagg
      961 gccaggtgcg ctgcgggcaa tgccaccagg tcttcaacgg cttcgaatcg ctggagcgct
     1021 tccccggcga cgataccggc gcgcggctgc tagccgcccg ggcggcaaac ggcacccatc
     1081 ccgatcccgt tgccgagctc gacgacgcgg acctcggtcg ccccgtggag cacgccgtgc
     1141 cttccgggca gccccccttc ctcgattcgc tgccgcagga ggcgtctccg gtgcccgtcg
     1201 gcccaccgga ctcctccggc cttatcgacg tgccggacct cctgccggaa agctcggtgt
     1261 ccaaggaggt gttgctcgaa gcaccctctg aagggcgctc gggagggccc ccggaaatgc
     1321 cctccgaagc gctcccggaa gcaccctaca agacagcggc gagggttcct cccgtcgtcg
     1381 tgaccgcgca gtccgtccgg cgcgcgcagc tcgcgcggga tggcgccttc gttgccgacc
     1441 cggccgcccg cccttcgcgg gcctgggcgt tcggcgtggc gctccttgcg ctggtgcttg
     1501 cgctgcaact cgcctacgcg tttcgcgcgc ccatcgcgca gcagtacccg gtgctgcgtc
     1561 cgatcctcga gtcggcgtgc gctgccgccg ggtgcagcgt accgtgggtc aacgacgagg
     1621 cggcgctcaa gctcgaggat tcggagctcc tcgaggtgcc cggcaagccc ggccagatcg
     1681 cgctgcaggc gcgcatacgc aatctcggga cgtccgcgca ggagtttccc cacctcgagc
     1741 tcacgctcac ggacgccacc ggccagactg cggtgcgccg ggtgctccgt cctgccgatt
     1801 atctggggcg cgtcccggcg gcgggcgaag tcatggccgc aggatcggaa gccctgctga
     1861 gcgtccgcct cgaaaccgga cgcatccgtc ccacggggta cgaattgctg ctgttctatc
     1921 cctgaacgcc ctcgcgcagg ctggcgagca tcgagtccac gacccggtcg acaagcggcg
     1981 cgtcgtcgac gatgcgcgcc tcgccacggc gcatcaactc ggccagcgct tccggcgaca
     2041 ccgagatcgc agcactcgcg ttggcgttgg tgaagaggta cacgcccctg gccgggctga
     2101 tccaggtgag ccgcgcgcga atcggctggg cgacctcgcg acggaactcg acccaggtgt
     2161 gtcttttcac gttcgtccat atgccgctgc gcgcgtacgc acccggcacc ggggtctggc
     2221 cgcgcggact gcgcagccgg atctcctcga tgtgcacgtc gccggcgggg accaacgctc
     2281 gctcgagcgc gggcgcaacg aacgcacgcg gctgccccgg ctccgggaca gtcggctgcc
     2341 cggcaagcac cgttcccttg aggccggccc ttaccgcgtc ggcgtggcag tcaaccagtt
     2401 caccgaggaa cgactcgctg atcgcgtccg cgaccccggc gcgcgacatc ccctcctgca
     2461 ggctgcggac gagggcggga atcctggcga cgagccggcg ccggtcctcc gtgcgcgact
     2521 tgcgctccac gctccacagc agttcatcga cggtggcgag catcgcggcc cactgcgcgc
     2581 agccctcgcc ctgctcgacg tgcacggtag caagtgcgcg cacccatatg tcttcgagca
     2641 tggcgcgcac cgcctccgga acccattcgc gcgcggcgat gcgccgatcc acttcgtcct
     2701 cggccaggac gcgcgccatc tcgaggcgct cgcgcccctc gacgagccgc gcggtgcgca
     2761 gtgcgatcgc ctcctccgcc tggtcctgcc gcgcaaggaa cgattccatg tccgccgcga
     2821 gcgtcgcgaa gacggcgagg tcgtcgtcga attcgtcgag cacgcggccg accacgacct
     2881 ccagcttcgc gagcgtatcg gccccgcgcg cgctcgattc atccaggccg atggccgcac
     2941 gcgcaagccg gtcgagcaac tgccgcgccg ggtgtgttcg cgtggagaag aacgtgcgat
     3001 cgagcagcgc caccttcagc gtcgggatct gcaggcgccc gagcagggcc ttcgcggcga
     3061 cgggaatctg gcggtcctcg aagacgaaat cgaagagcat cgcgacgatg tcgatcgtca
     3121 tcgcatcccc cccgaccagg atggaggact gcggcgactc cctgagttcg cggatcacgt
     3181 tgggtagccc ggtcgcgacg gaattcgcgc cggacccggg gagctgcagg ctggtgagca
     3241 atttcacgaa gccccgcgtg gccggagaaa ccgccggttc ttcgggcaag gccgacagcg
     3301 cgtccgcggg cgcggacgcg gcaaggagca gccgcgccag cgcggcgtgg acatcgggcg
     3361 cggggccggc accggtagtg gccggcgccc cgggttgcct gtcgtcgccg gttgccggcg
     3421 aggccgggct gcgccggtac gacggcctga tctccgggag aatctgccgc tccgcgagat
     3481 gcacattcag gtcgcggtag acgcggcgca gctctccggc cacctgcccc tcgagcacgc
     3541 gaagcagcgt gagccgcacc ttccagccgg cctcgatctg gtcgcacgct tccttgagcg
     3601 cgccgcagat cgtctcgggg ctcatcgggt tggcctcgtc ggcgaggtcg ggcttctcga
     3661 ggagatagcc gaagcgctgc gacagcatcg cgagctcccc ctcgcaagcg gccttgagcc
     3721 gcgccgccat gccctggacg gcgagcatgt gctcgagctc atcgtcatcc aggagggcga
     3781 gagcgcgcgg cggggcgcct gcgtcggccg ccgccggctc acccttcacc ttccggttga
     3841 agaactcgag gaactggcgg cggaacgcgg cctcgatgac cgggcgccgg ccgcgcgcct
     3901 gcgtgcgcgc atcgagatag agattctgcg tctcccggtc gcgcgagctc tcggcgagct
     3961 ggaaaagctc atcctcgacg cgatcgagca ccccggccag ggccttcacg attcgcccga
     4021 gcgcgaggtc gcggcaatcc gtgaggacgc ccgcggattc atgcggcgag agccgcgcgc
     4081 gggcgcgcgc atgcgcgtcg agggaa
//
LOCUS       DASUEV010000242        10151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1111017, whole genome shotgun sequence.
ACCESSION   DASUEV010000242 DASUEV010000000
VERSION     DASUEV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111017"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFV55_07805"
     CDS             <1..806
                     /locus_tag="VFV55_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4944245.1"
                     /translation="RTLWRHMFTRDEDRRRAFAIDAVMIEVNFTLGPAIIAGVLAAAN
                     TTTAFALAIGASVAALLVFAASPALRYFRAEKRSDRHLLGPLTEPRLLPVFIATFGLA
                     ASFGFLEVGYPGYATALAVPALGGIFLSVNSLGSALGGAVFGGLTLRAPIERQFAATT
                     GLMTAPLVLHYFVDAPAAFAFVAFLAGASIAPSIACQSVLVSRLAPSHYATEAFTWSS
                     TFIVSGLGAGMALGGWLIETTGPKTPFLAGAAIMVAVSLGALALPHPRK"
     gene            complement(1132..1857)
                     /locus_tag="VFV55_07810"
     CDS             complement(1132..1857)
                     /locus_tag="VFV55_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX4944246.1"
                     /translation="MKTQLLLSRVAVVLSRTSHPGNIGAAARAMKTMGIGDLVLVAPR
                     HFPHPEAVALAAGAGDVLQRARVVPTLEQALADRTLAIGFSARGRDLSHPARDWRSAA
                     PEAIAAAADGAVALVFGNETSGLSNDELLACQRHACIPANPDYPSLNLAAAVQVACYE
                     LALAASAFRIADRPPRAPATGADLEALHARLREVLVASGFLDPEEPGRLDERMRRLAA
                     RLDLESEEVAILHGMLGSLAPRK"
     gene            1923..2711
                     /locus_tag="VFV55_07815"
     CDS             1923..2711
                     /locus_tag="VFV55_07815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX4944247.1"
                     /translation="MHPMLTTAVKAARKAGAIITRASYDLERLTIRRKQHNDFVTEVD
                     HAAEDAIIRTLREAYPSHAFLAEESGASSAAANSDHLWIIDPLDGTTNFLHGFPQYCV
                     SIALKHKGVLQQAVVYDPNRNELFTATRGAGAFLNDRRLRVSATDRLEDALIGTGFPF
                     REFDQFDEYLRMFGEITRRVAGVRRPGAAALDLAWVAAGRLDGFWEMGLSPWDMAGGA
                     LLVREAGGLVGDLEGAEGFLDSGRIVAANSKVFAPLLQALAKRA"
     gene            complement(2723..3655)
                     /gene="menA"
                     /locus_tag="VFV55_07820"
     CDS             complement(2723..3655)
                     /gene="menA"
                     /locus_tag="VFV55_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEX4944248.1"
                     /translation="MPATRAAQYDDIPPGSLRAWAVAIRPRTLWIAAIPVAVGTAFAW
                     MHAATIEPWVAGLAFVASILMQVITNLQNDVGYTARGHESSRRVGFPRATAKGWLTPW
                     EVRRAIALAIACAVIVGTPLAIHSGPVVAAIGLASVLAALAYMGGPRPIAYTPLGELV
                     VFVFFGLVAVLGAYYLHAGPAGATVWLAAAAIGSHAAAVLVVNNHRDAEHDRRTGRRT
                     FAAAFGRRASAALYAIALWAPFALAAAMVAIDARPGLAAPLLLLPMAARLQSDFLNLP
                     AGPAFNLLLLRTVKLELAFGALLSAGAVLQRALS"
     gene            complement(3795..4628)
                     /locus_tag="VFV55_07825"
     CDS             complement(3795..4628)
                     /locus_tag="VFV55_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4944249.1"
                     /translation="MFTSIARALILLTLGAASIAFATPDKRGCTDHPLLPTRMPDYFL
                     SDCQTRDFDAYDFFVTKGPRHREEGKFFFITYQIEKGKTEQSALAIVRNYENAITKIG
                     GTIVASDQKNWVNGKVVVDGREIWLQAERGNGKIWLRIVEKAPMKQHIVADAAAFAND
                     LKATGHVAVYGIFFDTGKSVVKPESKAAIDEVAKLLKSDAALRLWVVGHTDSVGKVED
                     NMRLAQARAEAVAAELTGAYGIPAARLKGYGVGPLSPVAGNDSEEGRAKNRRVDLVKQ
                     P"
     gene            complement(4763..7207)
                     /locus_tag="VFV55_07830"
     CDS             complement(4763..7207)
                     /locus_tag="VFV55_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX4944250.1"
                     /translation="MATLSDWPIPPFFTVLPGDEVVAPENCLLLLVDGFRVTGRLVRF
                     APSQSSLTVLPDGVEEERTIPLDQVKCVRLLRRLRIRRQETPLETRAEELFPASERQN
                     YSVEFPDNEVIHGETLGYVNTRHGLYLFVPETDDGVMRHFYPRAAMRNYRIGLPIGEI
                     LVEEKVLSREAVDTALARQRELRTQRIGDYLTESRVVSREQLALAINHQEGKPLLRLG
                     EALMQLGLITQGQLDTALVRQREDRSIPLGRILTGMGLLDENTLKDALARKLGIPFVG
                     LAGFNFDPEAIRLVGASVARRRSLVPLCVHEGALVVAFEDPLDTAAIDEVRFLTQMRV
                     IPAMASRDEISRAIGKHYGQYGARPLPVPEADSGARSEYEFHRAADVDIDKLASRLTV
                     EDEALALTDEPAAESESILVQLVNRMILDAWSDGVSDIHIETFPGRKNTRVRFRKDGT
                     LVDYTEIPSGSRNAIISRIKVMAQLDISERRKPQDGKLDFRQFGPADIELRVATIPTS
                     NGLEDIVMRVLAASRPVAIEELRLAPDTLAHLKRLMERPYGLVLVCGPTGSGKTTTLH
                     SLLGSINTPGRKIWTAEDPIEITQPGLRQVQVNVKIGWTFAAAMRSFLRADPDVIMVG
                     EIRDAETARAGIEASLTGHLVLSTLHTNSAPESVVRLLDLGMDPFNFADALLAVLAQR
                     LAKSLCPHCRQRYRPEAPELEELAAEYCDGTDLKVEATLKRWRDEGAATGGVALFRAP
                     GCARCHDTGYLGRVGLHELLVMTPALRRLVQTRAPIGDLARAAMAGGMRTLKQDGIEK
                     VLGGLTDIGKVRAVCS"
     gene            7633..7932
                     /locus_tag="VFV55_07835"
     CDS             7633..7932
                     /locus_tag="VFV55_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEX4944251.1"
                     /translation="MNKKASRTKLVPFDAARYLTDDAAIAEYMSVVLETDDPDLLLLA
                     LGDVARARGMAQVAKDSGLGRESLYKALAPGAKPRFDTVMKVARALGVKFTARAA"
     gene            7983..>10151
                     /gene="mutS"
                     /locus_tag="VFV55_07840"
     CDS             7983..>10151
                     /gene="mutS"
                     /locus_tag="VFV55_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX4944252.1"
                     /translation="MMQQYLALKAGYPDMLLFYRMGDFYELFHADAERAAKLLDITLT
                     TRGASAGQPIRMAGVPYHAVEQYLAKLVKLGESVAICEQVGDPAASKGPVERKVVRVV
                     TPGTLTDSALLPATRESLLVSACVMEGTAGLAWLSLASGRLRVTEVPAGRLAAELERL
                     QPAELLVPDDFEPPPSRADAPVKRIASWQFDPPSAARELARQFGTADLSGYGAQELSA
                     ALGAAGALLAYARHTQQSALPHVTGLAVERESEFVQMDAHTRRNLEISETLTGGDSPT
                     LASVLDRCATSMGSRRLLHWLHHPLRDTAILARRHDGIEALLSGNSRVRHPAVSQALS
                     GWSDVERIAARVALGTARPRDLAGLRDTLATLPALHCLLSSAGTPALDRLAVPLAPVE
                     DVHADLAASLKEEPAVVVREGGVIANGFDAELDELRAIQADCGSFLLEMEKRERDRTG
                     IANLRVEFNRVHGFFIEVTQGQLDKVPADYRRRQTMKNAERFITPELKAFEDKALAAG
                     ERALAREKELYEALLARLQGHLARLQEIADAAAELDALAALAASAEALRLTRPAFTGE
                     DVIEIASGRHLVVESQVEDFIPNDTSLSRSRQLLLVTGPNMGGKSTYMRQVAQIALLA
                     YCGSFVPAAAATLGPLDRIFTRIGASDDLAGGRSTFMVEMTEAAAILNNATPRSLVLV
                     DEIGRGTSTFDGLALAYAIARHLAERVRCYALFATHYFELT"
BASE COUNT         1574 a         3547 c         3396 g         1634 t
ORIGIN      
        1 cgcgcaccct gtggcgacac atgttcactc gcgatgaaga ccggcgccgc gccttcgcca
       61 tcgacgcggt gatgatcgag gtcaacttca ccctcgggcc ggccatcatc gccggcgtgc
      121 tggccgcggc gaacaccacg accgcattcg ccctggcgat cggcgcgagc gtcgcggcgc
      181 tgctggtctt tgccgcctcg ccggcgctgc gctatttccg cgccgagaaa cgcagtgatc
      241 gtcacctgct gggcccgctc acggagcccc gcctgctccc ggtcttcatc gccacgttcg
      301 ggcttgcggc gagcttcggt ttcctcgagg tcggctaccc gggctacgcc acggcgctcg
      361 cagtacccgc gctcgggggc atcttcctgt cggtcaactc tttgggcagc gcgctgggtg
      421 gcgccgtctt cgggggcctc accctgcgcg cgccgatcga gcgccagttc gccgccacca
      481 cgggactgat gacggcgcct ctcgtgctgc actacttcgt cgatgcgccc gctgcgttcg
      541 ctttcgtcgc gttcctcgcg ggcgcgtcta tcgcgcccag catcgcctgc cagtcggtgc
      601 tcgtgtcgcg cctcgcgccc tcgcactacg cgaccgaggc attcacctgg tcctcgacct
      661 tcatcgtcag cgggctcgga gccggcatgg ccctcggggg ctggctcatc gagacgacgg
      721 gtcccaagac cccattcctg gccggcgccg caatcatggt cgccgtgtcc ctcggcgccc
      781 tcgctttgcc tcacccgcgc aagtgaaggg catggccgcc cgtccttcgg acccatcgat
      841 gattcgcatc tcctgctcgc acaggcatgg aggcaaacga agcccgacgc atggggtcag
      901 gttggttggg ttttttctta gttttcggcc aggtcaactt gcgtcgggcg taacggatcg
      961 aacctatgtc gaggtgcttc cggagtcgat gctggcagca atttcatctg gccgaaaact
     1021 aagaaagaaa cccaaccaac ctggccccgc cccgcctctg cgttcaccgc cgtgcaagat
     1081 tcgggaagat gcgaatcctt gcagaactcg gatggcgggc gaccgtgccc ttcacttgcg
     1141 cggtgcgagc gacccgagca tgccgtgcag gatggcgacc tcttcggatt ccaggtccag
     1201 gcgcgcggcc aggcggcgca tgcgctcgtc gagacgaccc ggctcctcgg ggtcgaggaa
     1261 gccgcttgcg accagcacct cgcgcagccg ggcgtgcagc gcctcgagat cggcgccagt
     1321 cgcgggcgcg cgcggtggcc ggtccgcgat gcggaaggcg gacgccgcga gggcgagttc
     1381 gtagcaggcc acctgcactg cagccgcgag attgagcgac gggtagtcgg ggttggcggg
     1441 gatgcacgcg tggcgctggc aggcgagcaa ctcgtcgttc gagagccccg acgtctcgtt
     1501 gccgaaaacc agggccacgg ccccatcggc cgccgcggcg atcgcctccg gggcggcgga
     1561 gcgccagtca cgcgccgggt gcgagaggtc gcgtccgcgc gcgctgaagc cgatggcgag
     1621 cgtgcgatcg gcgagcgcct gctccagcgt cggcaccacg cgcgcgcgct gcaggacgtc
     1681 gccggcgccg gccgccaggg ccaccgcctc cgggtgaggg aagtggcgcg gcgccacgag
     1741 cacgaggtcg ccaattccca tcgtcttcat ggcacgcgcg gccgctccga tgttgcccgg
     1801 atggctcgtt cgcgacagca ccaccgcgac gcgcgagagg aggagttgcg tcttcatcgt
     1861 ataattctgc ctgacgacac tttccggcgc cccgcccgcg cgggcgcgcc ccgctctttg
     1921 ccatgcaccc gatgctcacc acggccgtca aggccgcgcg caaggccggc gcgatcatca
     1981 cccgcgcctc ctacgacctc gagcgcctca ccatcaggcg aaagcagcac aacgacttcg
     2041 tcaccgaggt cgatcacgcc gccgaggatg cgatcatccg cacgctgcgc gaagcctacc
     2101 cgtcgcacgc cttcctcgcc gaggaaagcg gtgcctcgtc cgccgcggcg aactccgatc
     2161 acctgtggat catcgacccg ctcgacggca ccacgaactt cctgcacggc ttcccgcagt
     2221 actgcgtgtc gatcgcgctc aagcacaagg gcgtgctgca gcaggccgtc gtctacgacc
     2281 ccaaccgcaa cgaactgttc accgcgacgc gcggcgcggg ggccttcctc aacgaccggc
     2341 ggcttcgcgt gagcgcaacc gaccggctcg aggatgcgct gatcggcacc ggctttccgt
     2401 tccgcgagtt cgaccaattc gacgagtacc tgcgcatgtt cggcgagatc acgcgccgcg
     2461 tggccggcgt gcgccggccg ggcgcggccg ccctcgacct cgcctgggtt gcagccggtc
     2521 gcctcgatgg cttctgggag atgggactgt cgccatggga catggccggc ggcgcgcttc
     2581 tcgttcgcga ggccgggggc ctggtcggcg atctggaggg cgccgagggc ttcctcgaca
     2641 gcggccgcat cgttgcggcc aattcgaaag tctttgcacc gctgctgcag gcgctcgcca
     2701 agcgagcctg accccggcgc gatcaggaaa gggcgcgctg gagcacggcc cccgccgaca
     2761 gcaaggcgcc gaacgcgagt tcgagcttca cggtgcgcag gagcagcagg ttgaacgcgg
     2821 gacctgcggg cagattcagg aaatccgact ggaggcgcgc ggccatcggc aggagcagga
     2881 gcggcgcggc cagccccggg cgcgcgtcga tggcaaccat cgccgccgcc aatgcgaacg
     2941 gcgcccagag tgcgatggcg tagagcgcag ccgaggcgcg ccttccgaat gccgccgcga
     3001 atgtccggcg gcccgtccgc cggtcgtgct ccgcatcgcg atggttgttc accacgagca
     3061 ctgcggcggc atgcgaaccg atggcggccg cggccagcca aaccgtcgcc ccggccgggc
     3121 cggcatgcag gtaatacgcg ccgagcaccg ccacgaggcc gaagaagacg aacaccacga
     3181 gctcgcccag cggcgtgtac gcgatgggcc ggggcccgcc catgtaggcg agcgcagcca
     3241 gcacggatgc caggccgata gccgccacga cgggcccgga gtgaatggcg agaggcgtac
     3301 ccacgatcac cgcacaagca atggcgagcg cgatggcgcg ccgcacttcc cacggcgtga
     3361 gccatccctt cgccgtagcc cggggaaaac ccacgcgccg cgaggactcg tggccgcgcg
     3421 cggtgtagcc gacatcgttc tgcaggttgg tgatcacctg catgaggatc gaggcgacga
     3481 acgcgagtcc cgcgacccac ggctcgatcg tcgccgcgtg catccacgcg aatgcggtcc
     3541 cgaccgcaac gggaatcgcg gcgatccaca aggtgcgcgg gcggatggcg accgcccacg
     3601 cccgcagcga tccgggcggg atgtcgtcgt actgtgcggc gcgcgtggca ggcatcgggg
     3661 ttgcgaccag gggaacgcgc ggaattatac gacgcactgc cgcttccccg gggccggtga
     3721 agccacgacc ccgttacgcc ccgcctcctt cgccgctgac gagcgcctgg tcggcgcgca
     3781 tgagcgaggc ccggtcaggg ctgcttcacg agatccactc gccggttctt ggcccggcct
     3841 tcctcggagt cgtttccggc cacgggcgat agcgggccga ctccgtaacc cttgagccgc
     3901 gcggcgggga tcccataggc gccggtgagt tccgcggcca cggcctcggc gcgggcctgg
     3961 gctagcctca tgttgtcctc gaccttgccc accgagtcgg tgtgccccac gacccagagc
     4021 ctgagggcgg cgtcggactt gagcagcttg gcgacttcat cgatcgccgc cttcgactcg
     4081 ggcttcacca cggacttccc ggtgtcgaag aagattccgt agaccgcgac gtggccggtg
     4141 gccttcaggt cgttcgcgaa cgcggcggca tcggcgacga tatgctgctt catgggcgcc
     4201 ttctcgacga tgcgcagcca gatcttcccg ttgccccgct ccgcctgcag ccagatctcc
     4261 cggccgtcga cgacgacctt gccgttgacc cagttcttct ggtcgctcgc cacgatggtg
     4321 ccgccgatct tcgtgatggc gttctcgtag ttgcgcacga tggcgagcgc gctctgctcg
     4381 gtcttgccct tctcgatctg gtacgtgatg aagaagaact tgccctcttc gcgatgcctc
     4441 ggccccttgg tgacgaagaa gtcgtacgcg tcgaaatccc tcgtctggca atccgagagg
     4501 aagtagtccg gcatgcgggt cgggaggagc gggtggtcgg tgcagccgcg cttgtcgggc
     4561 gttgcgaagg cgattgacgc ggcccccagc gtcaggagga tgagcgctcg ggcgatgctc
     4621 gtgaacatgg tggtttcccg ttgatcgaag tgaagtgaag cggcacttca catgatcgcg
     4681 cccgggcatt gacggacgca agctcgggtg gcctttaggg gcatgcgggg ccggtcggcg
     4741 cgcgaaggct tggcgggctc cttcacgagc agacggcccg caccttgccg atgtccgtga
     4801 gcccgcccag gaccttctcg atgccatcct gcttgagcgt gcgcatcccg ccggccatgg
     4861 cggcccgcgc caggtcgccg atcggcgcgc gcgtctgcac gaggcgccgg agcgcgggcg
     4921 tcatgacgag cagctcgtgc agtcccacgc ggccaaggta gccggtgtcg tggcagcgcg
     4981 cgcaaccggg ggcgcggaaa agagcgacgc cgcctgtcgc ggcgccttcg tcgcgccagc
     5041 gcttcagcgt cgcctccacc ttcaggtcgg tgccgtcgca atactcggcg gcgagttcct
     5101 cgagttcagg cgcctcgggc cggtaccttt gccggcagtg cgggcacagc gacttggcca
     5161 ggcgctgggc gagaaccgca agcagcgcat ccgcgaaatt gaacggatcc atgccgaggt
     5221 cgagcaggcg caccacgctc tcgggcgcgc tgttggtgtg cagcgtcgac agcaccaggt
     5281 ggccggtgag cgaggcctcg atgcctgccc gggcggtctc ggcgtcgcga atctcgccga
     5341 ccatgatcac gtcggggtcc gcgcgaagga agctgcgcat ggccgcggcg aaggtccagc
     5401 cgatcttcac gttcacctgc acctgccgca ggcccggctg cgtgatctcg atcgggtcct
     5461 ccgccgtcca gatcttgcgg ccgggcgtgt tgatgcttcc gagcagggaa tgaagggtcg
     5521 tggtcttgcc cgagcccgtc ggaccgcaga cgaggaccag gccatagggc cgctccatca
     5581 ggcgcttcag gtgcgcgagc gtatccgggg caagacgcag ctcttcgatc gcgacggggc
     5641 gcgacgccgc cagcacccgc atcacgatgt cctccaggcc gttggacgtc ggaatcgtcg
     5701 cgacgcgaag ttcgatgtcg gcggggccga actgccggaa atcgagcttg ccgtcctggg
     5761 gcttcctgcg ctcggaaatg tccagctggg ccatcacctt gatgcgggag atgatcgcgt
     5821 tgcgcgaccc ggacgggatt tcggtgtagt cgaccagcgt gccgtccttg cggaagcgca
     5881 cccgggtgtt cttcctgccg gggaaagtct cgatatggat gtccgacacg ccgtcggacc
     5941 aggcgtccag gatcatcctg ttgaccagct ggaccaggat ggattccgac tcggcagccg
     6001 gctcgtcggt cagcgccagc gcctcgtcct ccaccgtgag tctcgaggcc agcttgtcga
     6061 tgtcgacgtc ggcggcgcga tggaactcgt attcgctgcg cgcgccggag tccgcctcgg
     6121 ggaccggcag cggcctcgca ccgtactggc cgtagtgctt gccgattgcc cgcgagatct
     6181 cgtcgcggga agccatcgcc gggatcaccc gcatctgcgt gagaaagcgc acctcgtcga
     6241 tcgccgcggt gtcgagcggg tcctcgaagg cgacgaccag cgcgccttcg tgcacgcaca
     6301 ggggcacgag cgatcgtctg cgcgcgacgc tcgcgccgac gagccggatc gcctcgggat
     6361 cgaaattgaa cccggcgagc ccgacgaacg ggatgcccag cttcctcgcg agcgcgtcct
     6421 tgagcgtgtt ttcgtccagg agccccatgc cggtgaggat cctgccgagc gggatgctcc
     6481 gatcctcgcg ctgccggacc agcgcggtat cgagttggcc ctgggtgatg aggccgagct
     6541 gcatcagcgc ctcgcccagg cgcagaagcg gcttgccctc ctggtgattg atggccaggg
     6601 caagctgctc ccgggagacc acccggctct cggtaaggta gtcgccgatg cgctgcgtgc
     6661 gcagttcacg ctgccgcgca agcgccgtgt cgaccgcctc gcggctcaac accttttcct
     6721 cgacgaggat ctcgccgatc ggcagcccga tccggtagtt gcgcatcgcg gcgcgcgggt
     6781 agaagtgccg catgacgccg tcgtccgtct ccggaacgaa aagatagagc ccgtgcctcg
     6841 tgttcacgta accgagcgtt tcgccgtgga tcacttcgtt gtccgggaac tccacggaat
     6901 agttctggcg ctcggacgca gggaacagct cctcggcacg cgtctcgagc ggggtctcct
     6961 ggcgccggat tcgcagccgc cgcaacaggc gcacgcactt gacctggtcc aggggaatcg
     7021 tgcgctcctc ctcgacgccg tcgggcagga ccgtgaggct ggattgcgac ggcgcgaagc
     7081 ggaccagccg gcccgtcacc cggaagccgt cgacaagcag cagcaggcag ttctccggcg
     7141 cgacgacttc atcgcccgga agaaccgtga agaacggcgg aatcggccaa tcggataatg
     7201 ttgccatcat cgtttcctcg ccccgtgcgc ctgcgccggg ccggaacccg gccgttcggt
     7261 cgcacgtcca aagtagcaca gggaatgccg cggccgccgc tagcctgcgt cgaggaaatg
     7321 ctcccgcatt tgggtcgcga ggagcggggg gagtccgatc tcgccgaccc ggtccggagt
     7381 tgatccttga aaaacccaca tgacaaaccc gtttttcaag ggcaatctgc ccctgtcgtt
     7441 cgcgagttcc tctggccaag cgaacacttg acatgtgtat ccactcggat acaatcggcg
     7501 aatgtatacg gtccgcctga cccggtattt tcaggattgg ctcgatgacc tgctgtgcgg
     7561 cggtgacaat tcgagtcaag agcgcgacat cagtcgtgtg cggcgcatcg ccagtgaact
     7621 ggagtccgaa tcatgaacaa gaaggcttct cgaaccaagc tggttccctt tgatgctgca
     7681 cgttacctca cggacgatgc tgcgatcgcg gagtacatgt ccgtcgtcct cgaaacagat
     7741 gatcctgact tgctgttgct cgcgcttggc gacgttgcac gtgctcgcgg aatggcgcag
     7801 gtggcaaagg attccggcct tgggcgcgaa agtctgtaca aggctctcgc tccaggagcc
     7861 aagccgcggt tcgacactgt catgaaggtc gcccgcgcgc tgggcgtgaa attcacggcg
     7921 cgcgccgctt gatgaggacg gcatcgtctt cggcatcggc aaagccggtt gaacacacgc
     7981 cgatgatgca gcagtacctc gccctcaagg cggggtaccc cgacatgctg ctcttctatc
     8041 gcatggggga cttctacgag cttttccacg cggacgccga gcgcgcggcg aaactgctcg
     8101 acatcacgct caccacgcgc ggcgcttccg ccggccagcc catccggatg gcgggcgttc
     8161 cctaccacgc cgtggagcaa tacctcgcga agctcgtgaa gctgggcgaa tccgtcgcga
     8221 tctgcgagca ggtgggcgac ccggcggcct cgaagggccc cgtcgagcgc aaggtggtcc
     8281 gcgtcgtgac gccgggcacc ctcaccgact cggccctcct ccccgctacc cgggagagcc
     8341 tcctcgtgtc ggcctgcgta atggagggta ccgcgggcct tgcgtggctc tcgctcgcga
     8401 gcggccgcct gcgcgtcacg gaggtgccgg caggccgcct ggctgccgag ctcgagcgac
     8461 tgcagcccgc agaactcctc gttcccgacg atttcgaacc accgccctcg cgcgcggacg
     8521 cacccgtgaa gcgcatcgcg tcgtggcagt tcgatccccc ctccgccgcc cgcgagctcg
     8581 cgcgccagtt cggcacggcg gacctctcgg gctacggcgc gcaggaactg tctgcagcgc
     8641 tcggcgctgc cggtgcgctc ctcgcctacg cgcggcatac gcagcagtcc gcgctgcccc
     8701 acgtcaccgg ccttgccgtg gaacgcgaga gcgaattcgt gcagatggat gcgcacacgc
     8761 gccgcaatct cgaaatcagc gagacgctga cgggaggcga ctccccgacg ctcgcctcgg
     8821 ttctcgaccg ctgcgcgacc tccatgggaa gccgccgcct cctgcactgg ctgcaccacc
     8881 cgcttcgcga cacggcgatc ctcgcgcgcc ggcacgacgg catcgaggcc ctcctgtcgg
     8941 gcaactcgcg cgtgcgtcac ccggccgtct cgcaggccct gtcgggttgg tcggacgtgg
     9001 agcgcatcgc ggcccgcgtg gcgctcggca cggcgcggcc gcgcgacctg gcggggctgc
     9061 gcgatacgct ggcgacgctc ccggccctgc actgcctgct gtcgtcggcg ggaacgcctg
     9121 cgctcgaccg cctcgccgtg ccgctcgctc ccgtggaaga cgtccacgcc gacctcgccg
     9181 cctcgctcaa ggaagagccg gccgttgtcg tccgcgaggg gggcgtgatc gcgaacggct
     9241 tcgacgcgga actggacgag ctgcgcgcca tccaggccga ctgcggctcg ttcctgctcg
     9301 agatggagaa gcgcgaacgc gatcgcacgg gcatcgccaa cctgcgcgtg gaattcaacc
     9361 gggtgcacgg cttcttcatc gaagtgacgc aggggcaact cgacaaggtg ccggccgact
     9421 accgccgccg ccagacgatg aagaatgcag agcgcttcat cacgccggag ctcaaggcct
     9481 tcgaggacaa ggccctcgcg gcgggggagc gggcgctcgc gcgcgagaag gaactctacg
     9541 aggcgctcct cgcgcgcctg cagggccacc ttgcgcgatt gcaggaaatc gccgacgccg
     9601 ccgccgagct ggacgccctg gccgcgctcg ccgcctccgc cgaagccctt cgcctcacgc
     9661 ggccggcgtt caccggcgag gacgtgatcg agatcgccag cgggcgacac ctggtcgtgg
     9721 aaagccaggt cgaggatttc attcccaacg acacgagcct ttcgcgctcg cgccagctcc
     9781 tcctcgtcac cgggccgaac atgggcggca agtccacgta catgcgccag gtggcgcaga
     9841 tcgcgctgct cgcgtactgc ggctcgttcg tgcccgcggc cgcggcaacc ctcggtccgc
     9901 tcgaccgcat cttcacccgc atcggcgcct ccgacgacct cgccggcggg cgctccacgt
     9961 tcatggtgga gatgaccgag gccgctgcga tactcaacaa cgcaacgcca cgcagcctgg
    10021 tgctggtgga cgagatcggg cgcggcacct ccaccttcga cggcctggca ctcgcctacg
    10081 ccatcgcgcg ccacctcgcc gagcgcgtgc gctgctacgc gctcttcgcg acgcactact
    10141 tcgagctcac g
//
LOCUS       DASUEV010000243         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1202530, whole genome shotgun sequence.
ACCESSION   DASUEV010000243 DASUEV010000000
VERSION     DASUEV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202530"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFV55_07845"
     CDS             <1..780
                     /locus_tag="VFV55_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944253.1"
                     /translation="AVACTFGVILGTPTLKLRGDYLAIVTLGFGEIIRIFLNNLSEPF
                     NLTNGPKGIATIDPVRFLGLDFASTTQIGALALSGTIKYYYFLLVVLLVVIVVNLRLQ
                     DSRIGRAWEAIREDEMAARAMGINTTRLKLLAFAMGASFGGIAGGMFSAIQGFISPES
                     FILTESVMVLSMVVLGGMGNIWGVVLGAVLLSFVPEVLRYTVEPVQRILFGRSLIDPE
                     VIRMLLFGLAMVVMMLFRPAGLLPSAVRKRELSVGRNNDDA"
     gene            767..1576
                     /locus_tag="VFV55_07850"
     CDS             767..1576
                     /locus_tag="VFV55_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944254.1"
                     /translation="MTTPETTGAAALLEIESVTKRFGGLTALSEVSFSIRPREIYGLI
                     GPNGAGKTTLFNVLTGLYPHDEGQFRFEGRRLVEVTPDRIAALGIARTFQNIRLFANL
                     SALENVMIGRHVRTSAGVVGAILRNPATRAEEAAIVRRARELLDYVGIGSRGNELARH
                     LAYGDQRRLEIARALATDPRLLALDEPAAGMNATEKQDLKKLLESIRRDGTTILIIEH
                     DMRLVMGLCDRVLVLDYGRRIAEGLPASVRRDPKVIEAYLGAQHHEASVSE"
     gene            1800..>2236
                     /locus_tag="VFV55_07855"
     CDS             1800..>2236
                     /locus_tag="VFV55_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4944255.1"
                     /translation="MSLLALEKVEVHYGGIRAVKGIDLTVEQGELVCLIGANGAGKTT
                     TLRAISGLANAGGRIVYDGEDIAGRKSHEIARKGIALVPEGRGVFPQLTIEENLAMGA
                     YTRNDRAAIAVDVERAFTLFPRLKERRRQTAGTMSGGEQQMLA"
BASE COUNT          363 a          782 c          702 g          389 t
ORIGIN      
        1 gcggtcgcct gcaccttcgg ggtgatcctg ggcacgccga cgctcaagct gcgcggcgac
       61 tacctcgcca tcgtgacgct gggcttcggc gagatcatcc gcatcttcct caacaatctc
      121 tccgagccct tcaacctcac caacgggccc aagggcatcg ccaccatcga ccccgtccgg
      181 ttcctcgggc tcgatttcgc ctcgacaacg cagatcggcg cgctcgcgct ctccgggacg
      241 atcaagtact actacttcct gctcgtcgtg ctcctcgtcg tcatcgtcgt gaacctgcgc
      301 ctgcaggatt cgcgcatcgg ccgggcgtgg gaggcgatcc gcgaggacga gatggccgcg
      361 cgcgcgatgg gcatcaacac cacgcgcctg aagctgctcg ccttcgccat gggcgcctcg
      421 ttcggcggca tcgccggcgg catgttctcc gccatccagg ggttcatcag cccggagagc
      481 ttcatcctca ccgagagcgt gatggtcctt tccatggtgg tgctgggcgg catgggcaac
      541 atctggggcg tggtcctggg cgcggtcctc ctctccttcg tgcccgaggt cctgcgctac
      601 accgtggagc cggtgcagcg catccttttc ggccgctcgc tcatcgaccc ggaggtgatc
      661 cgcatgctcc tcttcgggct ggcgatggtg gtgatgatgc ttttccggcc ggcagggttg
      721 ctgccctcgg ccgtacggaa gcgcgaactc tccgtgggcc gcaacaatga cgacgcctga
      781 gacaacgggc gcggcggcgc tgctcgagat cgagagcgtc accaagcgtt tcggcggcct
      841 caccgcgctc tcggaagtga gcttctccat ccgcccgcgg gagatctacg gcctcatcgg
      901 ccccaacggc gccggcaaga ccacgctctt caacgtgctc accgggctct accctcacga
      961 cgagggccag ttccgcttcg aggggcgccg gctcgtcgag gtcaccccgg atcgcatcgc
     1021 cgcgctcggc atcgcgcgca ccttccagaa catccgcctt ttcgccaacc tctcggccct
     1081 cgagaacgtg atgatcggcc gccacgtgcg caccagtgcg ggcgtcgtgg gcgccatcct
     1141 gcgcaacccg gcgacgcgcg cggaggaggc ggccatcgtg aggcgcgccc gcgagctgct
     1201 cgactacgtc ggcatcggct cgcgcggcaa cgagctcgcg cggcaccttg cctacggcga
     1261 ccagcgccgc ctcgagatcg cccgtgccct cgcaaccgat ccccgtctcc tcgccctgga
     1321 cgagcccgcg gccggcatga acgcgacgga gaagcaggat ctcaagaagc tgctcgaatc
     1381 catccgcagg gacggcacga cgatactcat catcgagcac gacatgcggc tcgtgatggg
     1441 cctgtgcgat cgcgtcctgg tcctggacta tggccggcgc atcgccgagg gtcttcccgc
     1501 ttccgtgcgc cgcgatccca aggtgatcga ggcctacctc ggggcgcagc accatgaagc
     1561 ttccgtcagt gagtgacgcg acccaaaggc agggacacgg atgaacacgg atgaacacgg
     1621 attttctgga atgtacggca ttgagattcg gcccgtgatc gtattggtga cgatcgctca
     1681 ggcgatgacc agtcatctcc tttcaaatat ccgtgttcat ccgtgtcccc gtttttggct
     1741 tccgccagcc acggtcggtg ttcatccgtg tccccgcttg tggcttccgt gagccaccga
     1801 tgagcctgct tgcactggag aaggtcgaag tccactacgg cggcatccgc gccgtgaagg
     1861 gcatcgacct caccgtggag caaggcgagc tggtctgcct catcggggcc aacggagcgg
     1921 gcaagacgac gacactgcgc gccatcagcg ggctggcgaa cgccggaggg cgaatcgtct
     1981 atgacggcga ggacatcgcg ggacgcaaat cccacgagat cgcgcgcaag ggcatcgcgc
     2041 tggttcccga gggccgcggc gtctttcccc agctcaccat cgaggaaaac ctcgcgatgg
     2101 gcgcctacac gcgcaacgac cgcgccgcca tcgcggtcga cgtcgagcgc gccttcaccc
     2161 tttttccgcg cctgaaggaa cgccgccgcc agacggccgg cacgatgtcc gggggagagc
     2221 agcagatgct tgccat
//
LOCUS       DASUEV010000244         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1111091, whole genome shotgun sequence.
ACCESSION   DASUEV010000244 DASUEV010000000
VERSION     DASUEV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111091"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VFV55_07860"
     CDS             <1..1113
                     /locus_tag="VFV55_07860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4944256.1"
                     /translation="LLRDGWSSFLRSPDPYVLLAVGLVQAYFLGSWQHAGRPRPFFGN
                     LIGPALYTLFEGIVEGFSGGMGFFLKPHHEAYWAFSIAIGILQHARLGAIDRWLAVAA
                     TLAENIVRVAIVVVMYAIFESITTPKYATFDGFLSDASHVYIGLMVLALGVVIGVARV
                     TSDSYLELLRSTAEKLRRYSELAMGRGVLERAVQNEAILAPTRRSRTVVFLDIRGFTA
                     WSERQTPEEVVRMLNAFYEAAEATVAPFAPIRIKYTADEALLVFDEPATAVAAARALR
                     AALAPQLAPFGLAAGFGISGGPVIEGLLGSESVKAFDVLGDTVNAAKRLCDAAGGGEI
                     LVAADLAIEGLADVPLREIQAKGKAQPISVRVLGTS"
     gene            1110..1928
                     /gene="prmC"
                     /locus_tag="VFV55_07865"
     CDS             1110..1928
                     /gene="prmC"
                     /locus_tag="VFV55_07865"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX4944257.1"
                     /translation="MTRTLRVALEESAEQVGAVDARVLACHLLDVGRAWLAANPMHVL
                     TESQDARLDLLVAQRALGQPVAYLVGSREFYGRDFEVGPAVLIPRPETETLVEAALER
                     MPPGGAVLDLGTGSGAIAVTLACERPQATVHATDASADSLAVARANAARHGARVEFAL
                     GSWYAPVAGRQFDLVVANPPYVAAGDHHLAEGDLRFEPRGALTDGSADGVDSIRAIVS
                     GAPGHLKSGGWLLIEHGYDQAAIVRALLEGAGLAGLVSIPDLSGIPRVAGGRRQ"
     gene            2031..2354
                     /gene="grxD"
                     /locus_tag="VFV55_07870"
     CDS             2031..2354
                     /gene="grxD"
                     /locus_tag="VFV55_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX4944258.1"
                     /translation="MSAQEKIRSTVTSNPVVLYMKGSPQAPMCGFSATATQILHLCGV
                     ENFTSVDVLQDAEVRQGIKEYANWPTIPQLYVNGEFVGGSDIMREMYQSGELQKLLEG
                     LKKPS"
BASE COUNT          348 a          891 c          846 g          380 t
ORIGIN      
        1 ctgctgcgcg acgggtggag cagtttcctg cgttcgccgg acccgtatgt gctgctcgcc
       61 gtggggctgg tgcaggcgta cttcctcggc tcgtggcagc acgcgggcag gccccgcccc
      121 ttcttcggca atctcatcgg cccggcgctc tatacgctgt tcgaggggat cgtcgagggc
      181 ttcagcgggg gaatggggtt cttcctgaag ccccaccacg aggcctactg ggcgttctcg
      241 atcgcgatcg ggatcctgca gcacgcccgc ctgggggcga tcgaccggtg gctggccgtc
      301 gcggccacgc tcgccgagaa catcgtgcgc gtcgcgatcg tcgtggtgat gtacgcgatc
      361 ttcgagtcga tcacgacgcc caagtacgcg acgttcgacg ggttcctctc cgatgcgagc
      421 cacgtctaca tcggcctcat ggtcctggca cttggcgtgg tgatcggcgt ggcccgggtc
      481 acctccgaca gctacctgga gctccttcgc tcgaccgccg aaaagctgcg ccgctattcc
      541 gagctcgcca tgggccgcgg cgtgctcgag cgcgccgtgc agaacgaggc gatcctcgcg
      601 cccacgcgcc gcagccgcac cgtcgtcttc ctcgacatcc gcggattcac cgcctggagc
      661 gagcgccaga cacccgagga agtggtccgg atgctcaacg cgttctacga ggcggcggag
      721 gcaaccgtgg cgcccttcgc gccgatccgc atcaagtaca cggccgacga ggcgctcctc
      781 gtcttcgacg agcccgcgac ggcggtggcg gccgcgcgcg cgctgcgcgc cgcgctcgcg
      841 ccgcagctcg ccccattcgg ccttgcggcc ggcttcggca tcagcggcgg gccggtcatc
      901 gaaggcctgc tgggcagcga gtcggtgaag gccttcgacg tgctgggcga cacggtgaat
      961 gcggccaagc gcctgtgcga cgcggccggg ggcggcgaga tcctcgtcgc ggctgacctt
     1021 gcgatcgagg ggctggctga cgtccccctt cgcgagatcc aggcgaaggg caaggcgcag
     1081 ccgatctccg tgcgcgtgct gggcacttcg tgacccggac cctgcgcgtc gcgctcgagg
     1141 aatcggccga gcaggtcggc gccgtcgacg cgcgcgtgct cgcctgccac ctgctcgacg
     1201 tggggcgggc gtggctcgcc gccaacccca tgcacgtcct caccgagtcg caggacgcgc
     1261 ggctcgacct tctcgtggcg caacgcgccc tgggccaacc tgtcgcctat ctcgtcggca
     1321 gccgcgagtt ctacggccgc gacttcgagg tggggcccgc ggtgctcatc ccccggcccg
     1381 agaccgagac gctcgtggag gccgccctcg agcgcatgcc ccccggcggc gcggtgctgg
     1441 acctcggaac cggcagtggc gccatcgcgg taacgctcgc gtgcgagagg ccgcaggcga
     1501 ccgtccacgc gaccgacgcc tcggcggact ctctcgcggt cgcccgcgcc aatgcggcgc
     1561 gccacggcgc cagggtcgaa ttcgccctcg ggagctggta cgcccccgtg gccgggcgcc
     1621 aattcgacct cgtggtcgcg aacccgccct acgtggccgc gggcgaccac cacctcgcgg
     1681 aaggggacct gcgcttcgaa ccccgcggcg cgctcaccga tggcagcgcg gacggcgtgg
     1741 actcgatccg cgccatcgtc tcgggcgccc cggggcactt gaaatccggc ggctggctcc
     1801 tcattgagca tgggtacgac caggcggcaa tcgtccgcgc gctcctcgag ggtgccgggc
     1861 tggccggtct cgtttcgatc cccgaccttt ccgggatccc gcgcgtcgcc gggggacggc
     1921 gacaatgacc ccggcaaccg tggggccatg acgggcaaag gcgaaattgc ccggccgcgt
     1981 cgtatcatga ttgcctgatt ccgaaccaac cgtcagcgaa agatccaccc atgagcgcac
     2041 aggaaaagat ccgcagcacc gtcacctcca accccgtcgt cctctacatg aagggctcgc
     2101 cgcaggcgcc gatgtgcggc ttctcggcaa ccgcgacgca gatcctccac ctttgcgggg
     2161 tggagaattt cacttcggtg gacgtgctgc aggacgccga ggtccggcag ggcatcaagg
     2221 aatacgccaa ctggcccacc atcccgcagc tctacgtgaa cggggagttc gtcggcggct
     2281 cggacatcat gcgcgagatg taccagtcgg gagagctgca gaaactgctg gaaggcctga
     2341 agaagccgtc ctgattcctc gccgcggcga ggccgcgcgc atgccgcgac tgctggccgc
     2401 cctcctggcc acgctgctcc tcggggcggc cccggccggc gcggcccccg gcgtggccgc
     2461 ggcct
//
LOCUS       DASUEV010000245         6283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1653202, whole genome shotgun sequence.
ACCESSION   DASUEV010000245 DASUEV010000000
VERSION     DASUEV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653202"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..3238)
                     /gene="carB"
                     /locus_tag="VFV55_07875"
     CDS             complement(32..3238)
                     /gene="carB"
                     /locus_tag="VFV55_07875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX4944259.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALRDEGYRV
                     ILVNSNPATIMTDPEMADVTYIEPITWRMVAKIIEKERPDALLPTMGGQTALNCALDL
                     AKHGVLEQYGVEMIGAKKEAIDKAEDREKFKLAMAKIGLASARSALAHSLEEALQVQA
                     SVGFPTIIRPSFTLGGTGGGIAYNREEFVAICERGLEASPTKELLIEESVIGWKEFEM
                     EVVRDMADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRNASIACLREIG
                     VETGGSNVQFAINPADGRMVIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LANDITGGATPASFEPTIDYVVTKVPRFAFEKFPQADDRLTTQMKSVGEVMAIGRNFQ
                     ESLQKALRGLEVGVDGFNLKSVDMEKIEDELAYPRAERLWYVADAFGIGMSLEEIHHF
                     TKIDPWFLAQIKDLVDTELAIENRSLADFPSDELRDLKRKGFSDRRLAHLWKTSEHQV
                     RARRHALGIRPVYKRVDTCAAEFATGTAYMYSTYDEEDEAEPTDRRKIMVLGGGPNRI
                     GPGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     DKEKPEGVIVQFGGQTPLKLARDLEAAGVPIIGTTPDSIDKAEDRERFQQLLHELKLL
                     QPPNRTARTPEAALEAAKEIGYPVVVRPSYVLGGRAMEIVHGEADLVRYMKEAVKVSN
                     DSPVLLDRFLNDAIEVDVDAVSDGTQVLIGAIMEHVEQAGVHSGDSACSLPPFSLSAE
                     LQDELRRQTVAMAKGLNVVGLMNVQFAIQDDKVFVLEVNPRASRTVPFVSKAIRTPLA
                     KVAARCMAGQSLASQGVTREIVPSYYSVKEAVFPFIKFPGVDTILGPEMKSTGEVMGV
                     GHTFAEAFVKSQLGAGVRLPKSGKAFLSVRDEDKPKLVEVGRTLAGLGFTLVATKGTA
                     AALEAAGLAVQRVNKVMEGRPNVVDMIRNREISLIINTVEEDRRSVRDSWSIRNSALQ
                     GRVTYYTTVSGARAACLGMQHLEELRPYDLQGLHATID"
     gene            complement(3421..4596)
                     /gene="carA"
                     /locus_tag="VFV55_07880"
     CDS             complement(3421..4596)
                     /gene="carA"
                     /locus_tag="VFV55_07880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX4944260.1"
                     /translation="MPEPSLSQKTPALLVLADGSVFRGTSIGAPGRTVGEVVFNTAMT
                     GYQEILTDPSYAGQVVTLTYPHIGNTGTNPEDVESRDVFAAGLVIRDLPLLASNWRMT
                     ESLPAFLERHGVVAIAGIDTRRLTRILRSRGAQNGCIVTGVAGHADAMVEADEKFALE
                     AARGAPSMAGLDLAKVVTCEARYEWNGSLWALGSGYGAGAEPRFHVVAYDFGIKHNIL
                     RHLAERGCRVTVLPAQTPAKEALACNPDGVFLSNGPGDPEPCTYAIEAIRDIVASGMP
                     TFGICLGHQLLGLASGAKTVKMKFGHHGANHPVQDLDTKRVFITSQNHGFAVDAATLP
                     GNVRATHKSLFDGSLQGFERTDRPAFSFQGHPEASPGPREMSYLFDRFIAMMAKRKS"
     gene            4742..5983
                     /locus_tag="VFV55_07885"
     CDS             4742..5983
                     /locus_tag="VFV55_07885"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX4944261.1"
                     /translation="MQALHALAGAAALLALCWLLSEDRRAVRWRVVASGVALMVALAA
                     LLLQVAWLRAAVFSLNGALEALERATQAGATFVFGYLAGGPAPFIETNPAASFVLAFR
                     ALPLILVVSALSALLFHWRILPVVVKAFAWLLNKSMGLGGAVGVSSAANVFVGMVEAP
                     LVVKPYLARLSRSELFMVMVCGMATIAGTVMALYGAILAPVVPDAIGHIIIASFVATP
                     AALVVAALMVPGGKSNEAAIEIPRQDASAMDAVTRGTVEGAQLLIQIVAMLIVLIALV
                     HLANAALSLLPALDGAPLTLQRMLGWLFAPLAWAAGVPWAEAQAGGALLGTKTILNEF
                     VAYLDLAKLPAEAMSVRSRLLMTYALCGFANFGSLGILIGGMGAMVPERRGEIVELGM
                     KSIVAGTLATCLTAAVVGLFV"
     gene            complement(6189..>6283)
                     /locus_tag="VFV55_07890"
     CDS             complement(6189..>6283)
                     /locus_tag="VFV55_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX4944262.1"
                     /translation="YAQGAIRAAKWVSGRAPGLYDMDDVLGLRG"
BASE COUNT          950 a         2045 c         2130 g         1158 t
ORIGIN      
        1 atcggaaatt tgcccatgcc cggattgttc ctcagtcgat cgttgcgtga aggccctgca
       61 ggtcgtaggg ccgcagttcc tcgaggtgct gcatgccgag gcacgccgcg cgcgcaccgg
      121 ataccgtcgt gtagtaggtg acgcggccct gcagcgcgga attgcggatc gaccatgaat
      181 cgcgcaccga gcggcggtcc tcctcgacgg tgttgatgat aaggctgatc tccctgttgc
      241 ggatcatgtc caccacgttg gggcggcctt ccatcacctt gttgacccgc tgcaccgcga
      301 ggcccgcggc ctcgagcgcc gcggccgtcc ccttggtggc caccagcgtg aagccgagcc
      361 cggcgagcgt gcggcccacc tcgaccagct tgggcttgtc ctcgtcgcgc accgacagga
      421 acgccttgcc ggacttcggc aggcgcacgc ccgcgccgag ctggctcttc acgaacgctt
      481 ccgcgaaggt gtggccgacc cccatgacct cgcccgtcga tttcatttcc ggaccgagaa
      541 tggtgtccac gcccgggaac ttgatgaacg ggaacacggc ttccttcacc gagtagtacg
      601 acggcacgat ctcccgggtc acgccctgcg aggcgagcga ctgcccggcc atgcagcgcg
      661 cggccacctt ggcgagcggc gtgcggatgg ctttcgagac gaaaggcacg gtgcgcgagg
      721 cgcgcgggtt cacttccagc acgaacacct tgtcgtcctg gatggcgaac tgcacgttca
      781 tgagcccgac cacgttcagc cccttcgcca tcgcgacggt ctggcggcgc aattcgtcct
      841 ggagctccgc ggagaggctg aagggcggca gcgagcaggc cgagtcgccg gaatggacgc
      901 ccgcctgctc gacgtgttcc atgatggcgc cgatgagtac ctgggtgccg tcgctcacgg
      961 cgtcgacgtc gacttcgatc gcgtcgttga ggaagcggtc gaggagcacg ggcgaatcgt
     1021 tggagacctt caccgcctcc ttcatgtagc gcacgaggtc ggcctcgccg tggacgattt
     1081 ccatggcgcg cccgcccagc acgtaggagg gccgcaccac gaccgggtag ccgatttcct
     1141 tggccgcctc cagcgccgcc tcgggcgtgc gcgcggtgcg gttgggcggc tgcaggagct
     1201 tcagctcgtg cagcaactgc tggaatcgct cgcggtcctc ggccttgtcg atggaatccg
     1261 gcgtcgtgcc gatgatgggc acgcccgccg cctccaggtc gcgcgcgagc ttgagcggcg
     1321 tctgcccgcc gaactgcacg atcacgccct cgggcttttc cttgtccacg atctcgagca
     1381 cgtcctcgag cgtgagcggc tcgaaataca ggcggtcgga ggtgtcgtag tcggtcgaga
     1441 cggtctccgg gttgcagttg accatgatcg tctcgtagcc gtcctcgcgc agcgcgagcg
     1501 ccgcgtgcac gcagcagtag tcgaactcga tccccgggcc gatgcggttg ggcccgccgc
     1561 ccagcaccat gatcttgcgg cgatcggtcg gctcggcctc gtcctcctcg tcgtaggtcg
     1621 agtacatgta ggccgtcccg gtggcgaatt ccgccgcgca ggtgtcgacg cgcttgtaga
     1681 cggggcggat ccccagggca tggcggcgcg cgcgcacctg gtgctcgctc gtcttccaca
     1741 ggtgggccag gcgccggtcg gagaagccct tgcgcttgag gtcgcgcagt tcatcggagg
     1801 ggaagtcggc cagcgagcgg ttctcgatgg cgagctccgt gtccaccagg tccttgatct
     1861 gcgcgaggaa ccacgggtcg atcttcgtga agtggtggat ctcctcgagc gacatgccga
     1921 tgccgaaggc gtccgccacg taccacaggc gctcggcgcg cgggtaggcg agctcgtcct
     1981 cgatcttttc catgtcgacc gacttcaggt tgaagccgtc cacgccgact tccaggccgc
     2041 gcagggcctt ctgcagcgac tcctggaagt tgcggccgat ggccatcacc tcgcccacgc
     2101 tcttcatctg cgtggtgagg cggtcgtcgg cctgcgggaa cttctcgaag gcgaaacgcg
     2161 gcaccttcgt caccacgtag tcgatggtgg gctcgaagga ggccggggtc gcgccgcccg
     2221 tgatgtcgtt ggccagctcg tccagcgtgt agcccacggc gagcttggcc gcgatcttgg
     2281 cgatggggaa gcccgtggcc ttggaggcga gcgccgagga acgcgacacg cgcgggttca
     2341 tctcgatgat gaccatgcgc ccgtcggccg ggttgatggc gaactgcacg ttggagccgc
     2401 ccgtttccac cccgatctcg cgcaggcacg cgatcgaggc gttgcgcatg atctggtatt
     2461 ccttgtcggt cagcgtctgc gccggggcga cggtgatgga atcgcccgtg tgcacgccca
     2521 tggggtcgag gttctcgatg gagcagatga tgatgcagtt gtccgccatg tcgcggacga
     2581 cctccatctc gaactccttc cagccgatga cgctctcctc gatgaggagc tccttggtgg
     2641 gcgaggcttc caggccgcgc tcgcagatgg cgacgaactc ctcccggttg taggcgatgc
     2701 cgccaccggt tccgccgagg gtgaaggaag gcctgatgat cgtggggaag cccacgctcg
     2761 cctgcacctg cagcgcctcc tcgagcgagt gggccagcgc cgagcgcgcg ctcgccaggc
     2821 cgatcttcgc catcgccagc ttgaacttct cgcggtcctc ggccttgtcg atggcctcct
     2881 tcttggcgcc gatcatctcc acgccgtact gctcgagcac gccgtgcttc gcgaggtcga
     2941 gggcgcagtt gagcgcggtc tgcccgccca tggtgggcag cagcgcatcg gggcgctcct
     3001 tctcgatgat cttcgcgacc atccgccagg tgatgggctc gatgtaggtg acgtcggcca
     3061 tctccgggtc ggtcatgatc gtggccgggt tcgagttcac caggatcacc cggtagccct
     3121 cgtccctcag cgccttgcag gcctgcgcgc cggagtagtc gaactcgcac gcctggccga
     3181 tgacgatcgg gccggcgccg atgatcagga tgctcttgat gtcggtgcgc ttaggcatgc
     3241 gctgctaccc cgggaacacg gatgaacacg gatatccacg gatgaacacg gatgagatcg
     3301 ccattgatcc gggggacgga ctcgcaccgc cgatcacccg tcgtggtcat cggaccgaca
     3361 acgtcaattc gacttgaagt atcggtgttc atccgtggat atccgtgttc atccgtgttc
     3421 ctaagacttt ctcttcgcca tcatcgcgat gaacctgtcg aagaggtacg acatctcgcg
     3481 cggccccggg ctcgcctcgg ggtggccctg gaagctgaaa gcggggcggt cggtgcgctc
     3541 gaagccctgc agcgagccgt cgaagagcga cttgtgcgtg gcgcgaacgt tgccgggaag
     3601 cgtggccgcg tccaccgcga agccgtggtt ctggctggtg atgaagacgc gtttcgtgtc
     3661 gaggtcctgc accggatggt tggcaccatg gtggccgaac ttcatcttca cggtcttcgc
     3721 accgctcgcg agcccgagca actggtggcc gaggcagatg ccgaacgtgg gcatgccgga
     3781 ggcaacgatg tcgcggatgg cctcgatcgc gtaggtgcag ggttccgggt cgccgggacc
     3841 gttggagagg aacacgccgt cggggttgca ggcgagcgcc tccttcgcgg gagtctgcgc
     3901 gggcagcacg gtgacacggc agccgcgttc ggccaggtgg cgaaggatgt tgtgcttgat
     3961 cccgaagtcg taggcgacga cgtggaagcg cggctcggcg ccggcgccgt aacccgagcc
     4021 aagcgcccag agcgagccgt tccactcgta gcgcgcttcg cacgtcacca cctttgccag
     4081 gtccaggccg gccatcgagg gggcgccgcg cgcggcctcc agggcgaact tctcgtcggc
     4141 ctcgaccatc gcgtcggcgt ggccggccac accggtgacg atgcagccgt tttgcgcgcc
     4201 cctgctgcgc aggatgcggg tcaggcgccg cgtgtcgatg ccggcgatgg ccacgacgcc
     4261 gtgccgttcg aggaaggcgg gaagcgactc cgtcatccgc cagttcgacg cgaggagggg
     4321 aaggtcgcgg atcacgagac cggcggcgaa gacgtcgcgc gattcgacat cctcggggtt
     4381 ggtgccggtg ttgccgatgt gcgggtaggt gagcgtgacc acctggccgg cataggacgg
     4441 atcggtgagg atctcctggt agccggtcat ggcggtattg aagaccacct cgcccacggt
     4501 gcggccgggg gcgccgatgg aagtgccacg aaagacggac ccgtcggcta gcaccaggag
     4561 ggcgggggtc ttctgcgaca aggacggctc cggaataacg aggggaaacg acgggcgcaa
     4621 aaaaacggga ggagcttttg cgtagtgcag gctcatcccg ctttcattag cctgaaatca
     4681 tagcaaatta tcgccctttc ttcaacgaac gcgcggctcc ggaggataat tcccgcacat
     4741 catgcaagcc cttcatgcgc tcgccggcgc cgctgccctc ctcgcccttt gctggctcct
     4801 ttcggaggac cgcagggcgg tgcgctggcg cgtcgtcgcc tcgggtgtgg cgctcatggt
     4861 ggccctcgcc gccctcctcc tccaggtggc gtggctgcgc gccgccgtct tctcgctcaa
     4921 tggcgcgctc gaggcgctgg agcgcgcgac ccaggccggc gccactttcg tcttcggcta
     4981 cctcgcgggc gggccggcgc ccttcatcga gaccaatccc gcggcttcgt tcgtgctcgc
     5041 gttccgggcg ctgccgctca tcctcgtggt gagcgcgctc tccgccctgc ttttccactg
     5101 gcgaatcctt ccggtggtgg tgaaggcctt cgcgtggctc ctgaacaagt cgatgggcct
     5161 cgggggtgcg gtcggcgtct cgtcggcggc caacgttttc gtgggcatgg tagaggcgcc
     5221 gcttgtcgtg aagccctacc tcgcgcgcct ctcgcgatcc gagctcttca tggtcatggt
     5281 gtgcggcatg gcgaccatcg ccgggacggt gatggcgctc tacggcgcga tcctggcgcc
     5341 cgtggtgccg gatgcgatcg gccacatcat catcgcctcg ttcgtcgcca cacccgccgc
     5401 cctcgtggtg gcggccctga tggtgcccgg cgggaagtcg aacgaggccg ccatcgagat
     5461 cccgcgccag gacgcgagcg cgatggacgc cgtcacgcgc ggcaccgtgg aaggagcgca
     5521 gctcctcatc cagatcgtgg ccatgctcat cgtcctgatc gcgctcgtgc acctggcgaa
     5581 cgcggcgctt tcactcctgc ccgcgctcga cggcgctccc ctcaccctgc agcgaatgct
     5641 cgggtggctc ttcgcgccgc tggcctgggc agcaggcgtg ccgtgggcgg aggcgcaggc
     5701 gggcggggcg ctcctcggaa cgaagacgat cctgaacgag ttcgtggcct acctcgacct
     5761 ggcgaagctt cctgcggagg cgatgagcgt ccgctcgcgg ctgctgatga cctatgcgct
     5821 gtgcggcttc gccaacttcg gcagcctcgg catcctcatc ggcggcatgg gcgcgatggt
     5881 tcccgagcgg cgcggggaaa tcgtggagct ggggatgaaa tcgatcgtgg ccggaacact
     5941 cgccacatgc ctgaccgcgg cggtcgttgg tttgttcgtg taggacggcg gggggagtga
     6001 tgcgggcgta aacctttgga acacggatga acacggatag gggctagtga tttcccgagt
     6061 ctgaaatcag tttcggaatt tcaatcactc gttgtccgat cgccatccga taacgaaata
     6121 tcagctccta tccgtgttca tccgccttta tccgtgttca tccgtgttcc aaaggtttac
     6181 gccaaccgtc agccacgcag cccaagaacg tcgtccatgt cgtagagccc cggtgcgcgg
     6241 ccggaaaccc atttcgccgc acggatcgcg ccctgtgcat aag
//
LOCUS       DASUEV010000246         7375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1317994, whole genome shotgun sequence.
ACCESSION   DASUEV010000246 DASUEV010000000
VERSION     DASUEV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317994"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VFV55_07895"
     CDS             complement(<1..1655)
                     /locus_tag="VFV55_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4944263.1"
                     /translation="MTSLTRRAFLKSSVAAASAATVGLPVSQAVLAQAKAAEAGWAWD
                     KAVCRFCGTGCGIQVATQGGRIVATKGDPDAPVNRGLNCIKGYFNGKILYGADRLTQP
                     LMRMKDGKFDKKGPFVPVTWQVAMDEMERQFRKAYGEKGPSGVAIIGSGQYTIQEGYV
                     AAKLMKAGFRSNNIDPNARHCMASAVAAFMQTFGIDEPANIYDDIELADTMVLWGANM
                     AEAHPVLWSRITDRKLAARPTKLVNLTTYRNMSSDIADLEIVMAPSSDLAIQNYIARE
                     LIARNAVNWDFVKKHCVFATGPYDIGYGMRPTSKFAYDAEKDTQAKELEVTLGKAEAI
                     AQRRQPGEKVAQKNRTSAGKHWLISFEEFKKGLEPYTLDFVAEIARGDPDEPMDKFKA
                     KLVQLADLYADSKRNTLSFWTMGFNQHQRGVWVNEQNYMIHLLLGKHGTPGNGAFSLT
                     GQPSACGTAREVGTFAHRLPADMVVDNPAHRALTEKTWKLPAKTLNPKVGSDIMAILR
                     DLEDGAVKFLWVQVTNPFQSSPNANHWMKAAREMDNFIVVADC"
     gene            complement(1652..1900)
                     /locus_tag="VFV55_07900"
     CDS             complement(1652..1900)
                     /locus_tag="VFV55_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HEX4944264.1"
                     /translation="MNLSGILVVVPPERFDDCVADLGTLPGVEVHNTDPATGRVIVVQ
                     EAADVDAEIAGLLAIKARPHVRAADMVYHYFANEGEET"
     gene            complement(2058..3470)
                     /gene="hemG"
                     /locus_tag="VFV55_07905"
     CDS             complement(2058..3470)
                     /gene="hemG"
                     /locus_tag="VFV55_07905"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX4944265.1"
                     /translation="MDTSPAALDVLVVGAGISGLAAAFGLARRGLSVEVLEAASRPGG
                     VIGSVRREGCMYETGPNSALDTTPLIGELIEAAGIAPERLPASEIANTRFVVRYGRPI
                     ALPMSPGAFLATPLFSPLAKARLLLEPLIARQPADREEVIAHFVRRRLGREFLDYAIE
                     PFVAGIYAGDPERLSVPAAFPKLHALEQRYGSLILGAILGARERRKRAETAKNTAKSF
                     SFRNGMQALTDALARGVGRVTLGARVVSLSRRSDGNFIVVFDKDGETIARHARSVVLA
                     VSAGDAARLAYPLAPDATRALQEIEYPPVTVVATAYRREDVTHSLGGFGLLAPRVEGR
                     AVLGTLFSSSMFAGRAPEGQVLLTNFVGGRRNPELASADDGILLEKVAAEHRTLLGAR
                     APALWNAVTRWPRAIPQYERGHLARMAKVAEAERAVPGLKFCANYRGGVSVGDCIASA
                     HATAESLGAWLRAPVTVRAA"
     gene            3583..4476
                     /locus_tag="VFV55_07910"
     CDS             3583..4476
                     /locus_tag="VFV55_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4944266.1"
                     /translation="MAQQDALFPGFRAVDLDAAGVAVHAVVGGNGPPLLLLHGYPQTH
                     ALWHKVAPRLAERYTVVAPDLRGYGDSGKPATDARHAPYSKREMAKDPVAAMRALGFG
                     RFFLAGHDRGARVAHRLAMDHGDAVEKVAVLDISPTLAMYEQTDMAFARAYWHWFFLI
                     QPAPLPETMIGADPVFYLERKMGSGAAGVAPFAPEAWKEYVRCFTPETIHASCEDYRA
                     AATIDLEHDRADRDAGRRIACPLLALWGANGVVERCFKPLEDWSRVAADVRGHALPAG
                     HYLPEEVPDLVAAEFETFFGA"
     gene            4483..5538
                     /locus_tag="VFV55_07915"
     CDS             4483..5538
                     /locus_tag="VFV55_07915"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX4944267.1"
                     /translation="MKTYRIATIPGDGIGNEVVPEGVRVIEAAGRRFGIAFTWDEKPW
                     SCAYYAKHGCMMPEDGLAQIRNHDAIFLGAVGFPGVPDHVSLWGLLVPIRREFQQYVN
                     LRPVRLMPGVKSPLAGRNPGDIDFWVVRENNEGEYSSMGGRMYGGTEQEFVTQLSVFS
                     KRGTDRIMRYAFELAKTRKRKHVTSATKSNGIAITMPYWDERFKAIAAEYPEVKTSHY
                     HIDILTAHFVQHPDWFDVVVGSNLFGDILSDLGPACTGTIGIAPGGNINPEREFPSMF
                     EPVHGSAPDIAGKGIANPIGQIWSGAMMLEHLGHKDAADAIVGAIEKVLADGPRTRDL
                     GGTANTVEVGKAIAAAL"
     gene            complement(5548..6450)
                     /locus_tag="VFV55_07920"
     CDS             complement(5548..6450)
                     /locus_tag="VFV55_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX4944268.1"
                     /translation="MDLRDLRYFEAIAETGHVGRAASKMFRTQPALTGSVRRLEAALG
                     TSLFERVGRGIRLTAAGEALVHRARALRIAAEDTVREIGELGSGVAGQVRIGVLPTLA
                     RFLMPPLCRVFLQQAPAVTIKTVIAQNDVLGGHLEAGEIDLMVSTGTKAAADLAIHPI
                     LRDEAVVIASRSHPVLRRKARIQDLLAYRWVLAPTSVGTRQWLERAFHSRGLPGPDVQ
                     IETNLILMMPTLIRRTELLTFTSRRHTSRIDAGATLAEVRVRETTMPRRFDVVYRRDG
                     YLSPAAKKMLALLRARGEELFAEE"
     gene            6592..>7375
                     /locus_tag="VFV55_07925"
     CDS             6592..>7375
                     /locus_tag="VFV55_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX4944269.1"
                     /translation="MNRWATRAVAAAAFVLTAGFSGAALAQAGYPDKPVRMIVPFPPG
                     GGTDILGRIVGDKLSSTLGWKVVIENKAGAGGTVGLDAAAKARPDGYTIVMAQTSNLS
                     IAPSIMSGLPYDPVKNFMPVRLVASVPLAIVVGKDSPFRSLADLVAAAKASPGKLLFA
                     SPGNATVAHLAGEYFQKVAGVKYTHVPYKGTGQALPDVISGRASFYVASIESAMPQVK
                     AGQLRAIAVTSVKRAPQWPDVPTVAESGYAGFEAITWFGIAVP"
BASE COUNT         1140 a         2479 c         2558 g         1198 t
ORIGIN      
        1 tagcagtcgg ccacgacgat gaagttgtcc atctcgcgcg cggccttcat ccagtggttg
       61 gcgttgggcg aggactggaa cgggttggtc acctgcaccc agaggaactt gacggccccg
      121 tcctcgaggt cgcgcaggat cgccatgatg tcgctgccga ccttcgggtt gagcgtcttc
      181 gcggggagct tccacgtctt ttccgtgagc gcccggtgcg cagggttgtc cacgaccatg
      241 tcggccggca gccggtgcgc gaacgtgccg acctcgcgcg cggtgccgca ggccgacggc
      301 tgcccggtga gcgagaaggc gccgttgccc ggcgtgccgt gcttgcccag cagcaggtgg
      361 atcatgtagt tctgctcgtt cacccacacg ccgcgctggt gctggttgaa gcccatcgtc
      421 cagaaggaca gggtgttcct cttcgagtcg gcatagaggt cggcgagctg cacgagcttc
      481 gccttgaact tgtccatcgg ctcgtcgggg tcgcccctgg cgatctcggc gacgaagtcg
      541 agcgtgtagg gctccagccc cttcttgaat tcctcgaagg agatgagcca gtgcttgccg
      601 gcgctggtgc ggttcttctg cgccaccttc tcgccgggct gcctgcgctg cgcgatcgcc
      661 tcggccttgc ccagcgtgac ctcgagttcc ttcgcctgcg tgtccttctc ggcgtcgtaa
      721 gcgaacttgg aagtcggccg catgccgtac ccgatgtcgt acgggccggt ggcgaagacg
      781 cagtgcttct tcacgaagtc ccagttcacg gcgttgcgcg cgatgagctc gcgggcgatg
      841 tagttctgga tcgcgaggtc ggaggagggc gccatcacga tctcgagatc cgcgatgtcg
      901 gagctcatgt tgcggtaggt ggtgaggttc accagcttcg tcggcctggc ggcgagtttc
      961 cggtcggtga tgcgcgacca aagcaccggg tgcgcctcgg ccatgttggc gccccacagg
     1021 accatggtgt cggcgagctc gatgtcatcg tagatgttgg ccggctcgtc gatgccgaag
     1081 gtctgcatga aggccgccac cgcgctcgcc atgcagtggc gcgcgttggg gtcgatgttg
     1141 ttggagcgga accccgcctt catgagcttg gcggcgacgt agccctcctg gatcgtgtac
     1201 tggcccgagc cgatgatcgc cacccccgag ggtcccttct cgccgtaggc cttgcggaac
     1261 tggcgctcca tctcgtccat ggccacctgc caggtgacgg ggacgaacgg ccccttcttg
     1321 tcgaacttgc cgtccttcat gcgcatgagc ggctgcgtga gccggtcggc accgtagagg
     1381 atcttgccgt tgaagtagcc cttgatgcag ttgaggccgc ggttcacggg agcgtcgggg
     1441 tcgcccttgg tggcgacgat cctgccgccc tgggtcgcga cctggatgcc gcagccggtg
     1501 ccgcagaagc ggcagacggc cttgtcccag gcccatcccg cctcggcggc cttcgcttgc
     1561 gcaaggaccg cctggctgac cggcaggccc accgttgcgg cggaggcggc ggcgacgctc
     1621 gacttgagga acgcgcggcg tgtcaggctc gtcatgtttc ttctccctca ttggcgaagt
     1681 agtggtagac catatcggcc gcccgcacgt gggggcgtgc cttgatcgca aggagtccgg
     1741 cgatctcggc gtccacgtcg gcggcttcct gcacgacgat gacgcggccg gtggcgggat
     1801 cggtgttgtg gacctccacg ccgggaagcg tcccgaggtc ggcgacgcag tcgtcgaacc
     1861 gttcgggcgg gacgacgacg aggatgccgg agaggttcat cggggagggt cccgggaggg
     1921 accttcatcc gtggtggcgg aattaccaac cattggagac ccgcgcaatc ggcgcccggt
     1981 tgaccccgat caaaacatga ctggcccatg cccctttagg ggcacgcctc gccggcccgg
     2041 cgaagaatcg tcccgggcta cgcggcccgc accgtgacgg gcgcccgcag ccaggcaccc
     2101 agcgattcgg cggtggcgtg ggcgctggcg atgcagtccc cgacggaaac gccgccgcga
     2161 tagttggcgc agaacttcag gcccggcact gccctttccg cctcggcgac cttggccatg
     2221 cgcgccaggt ggccgcgttc gtactgcggg atggcgcgcg gccagcgcgt caccgcgttc
     2281 cacagcgccg gcgcgcgggc gccgagcagg gtccggtgct cggccgcgac tttctccagg
     2341 aggatcccgt cgtccgccga ggcgagctcc gggttgcgcc ggccgcccac gaagttggtg
     2401 aggagcacct gtccctcggg cgcgcgaccc gcgaacatcg agctggagaa gagggttccc
     2461 agcaccgcac ggccttcgac gcggggcgcg aggagcccga atccgccgag ggaatgggtg
     2521 acgtcctcgc ggcggtaggc ggtcgcgacc accgtgaccg gcgggtattc gatctcctgg
     2581 agcgcgcggg tggcatccgg cgcgagggga taggccaggc gcgcggcgtc gcccgccgat
     2641 accgcgagca ccacggagcg ggcatgacgc gcgatggtct cgccgtcctt gtcgaacacg
     2701 acgatgaagt tgccgtcgga ccggcgcgaa agggaaacga cccttgcgcc cagcgtcacg
     2761 cgccccacgc cgcgggccag tgcgtcggtg agggcctgca tgccgttgcg gaacgagaag
     2821 ctcttggccg tgttcttggc ggtctcggcg cgcttgcggc gttcccgggc gccgaggatg
     2881 gcgccgagga tcaggctgcc atagcgctgc tcgagggcgt gcagcttggg gaacgccgcg
     2941 ggaaccgaca gccgctcggg atcgccggcg tagatgcctg ccacgaaggg ctcgatggcg
     3001 tagtcgagaa actcgcgccc cagccgccgg cgcacgaaat gcgcgatgac ttcctcccgg
     3061 tccgccggct ggcgcgcgat caggggctcg aggagcaggc gcgccttcgc caggggcgag
     3121 aagagcggcg tggcgagaaa ggctccggga gacatcggca gcgcgatcgg gcggccatag
     3181 cggaccacga accgcgtgtt ggcgatctcg ctcgccggca ggcgctcggg ggcgattccc
     3241 gccgcctcga tcagttcgcc gatgaggggc gtcgtatcca gcgcgctgtt ggggccggtc
     3301 tcgtacatgc agccttcgcg ccgcaccgaa ccgatcacgc caccgggcct ggatgccgct
     3361 tcgagcacct cgaccgacag gccgcgccgc gcgaggccaa aggcggcggc gaggccggag
     3421 atgccggcac ccacgacgag gacgtcgagg gcggcagggg atgtgtccat ggtgttcggt
     3481 ccgtcggtca tttcgggaag cgtccttttt aggctattgc ggcgcgccgc cgttgattgg
     3541 catcaaggga ctggggggca cggggcgcag cataatggca ccatggccca gcaggacgcg
     3601 cttttccccg gcttccgcgc cgtcgatctc gatgccgcgg gcgttgcggt gcacgcggtc
     3661 gtgggcggca acggcccgcc gctcctgctg ctccacggct atccgcagac ccatgcgctc
     3721 tggcacaagg ttgcgccgcg ccttgcggag cgatacaccg tcgtggcccc cgacctgaga
     3781 ggttacgggg actcgggcaa gcccgccacc gacgcgcgcc acgccccgta ttccaagcgc
     3841 gagatggcga aggaccccgt cgccgccatg cgcgcgctgg gcttcgggcg tttcttcctc
     3901 gcgggccacg accgcggcgc acgcgtcgcc caccggctcg ccatggacca cggggatgcg
     3961 gtcgagaagg tggccgtgct cgacatctcg cccacgctcg ccatgtacga gcagaccgac
     4021 atggccttcg cgcgcgccta ctggcactgg ttcttcctca tccagcccgc gcccttgccc
     4081 gagacgatga tcggcgccga cccggtcttc tacctggagc ggaagatggg cagcggcgcg
     4141 gcgggcgtgg cacccttcgc gccggaagcg tggaaggaat acgtgcgctg cttcacgccg
     4201 gaaaccatcc acgcgagctg cgaggactac cgcgctgccg ccaccatcga cctcgagcac
     4261 gaccgcgccg atcgcgacgc gggccggcgg atcgcctgtc cgctcctcgc gctgtgggga
     4321 gcgaacggcg tggtggaacg ctgcttcaag ccgctcgagg actggtcgcg cgtcgcggcg
     4381 gacgtgcgcg gccatgcgct tcccgcgggg cactatcttc ccgaggaggt ccccgacctc
     4441 gtcgcggccg aattcgagac tttcttcgga gcctgagaaa ccatgaagac gtacaggatc
     4501 gcaaccatcc ccggcgacgg gatcggcaac gaagtggtgc ccgagggcgt gcgcgtcatc
     4561 gaggcggcag ggcgccggtt cggcatcgcg ttcacctggg acgaaaagcc gtggagctgc
     4621 gcgtattacg cgaagcacgg ctgcatgatg ccggaggacg ggctcgcgca gatccgcaac
     4681 cacgacgcga tattcctggg cgccgtgggc ttcccgggcg tgcccgacca cgtctcgctc
     4741 tggggccttc tcgtgcccat ccgccgcgag ttccagcagt atgtgaacct gcggccggtg
     4801 cgcctgatgc ccggcgtgaa gagcccgctc gccggccgaa acccggggga catcgatttc
     4861 tgggtggtgc gcgagaacaa cgagggcgag tactcctcga tgggcgggcg catgtacggc
     4921 ggcacggagc aggaattcgt cacccagctt tccgttttct cgaagcgcgg cacggacagg
     4981 atcatgcgct atgccttcga actggcgaaa acgcgcaaga ggaagcatgt cacctccgcc
     5041 accaagtcca acggcatcgc gatcaccatg ccgtactggg acgagcgctt caaggccatc
     5101 gcggccgagt acccggaggt gaagacctcg cactaccaca tcgacatcct caccgcgcac
     5161 ttcgtgcagc acccggactg gttcgacgtc gtggtcggct cgaacctctt cggcgacatc
     5221 ctctccgacc tcgggcccgc ttgcacgggc accatcggca tcgcgcccgg cggcaacatc
     5281 aacccggagc gcgagttccc gtcgatgttc gagccggtgc acggcagcgc gccggacatc
     5341 gcgggcaagg gtatcgccaa tcccatcggc cagatatggt ccggcgccat gatgctcgag
     5401 cacctgggcc acaaggatgc cgccgacgcc atcgtgggcg cgatcgagaa ggtgcttgcg
     5461 gacggtccgc gcacgcgcga cctgggcggc acggcgaaca ccgtcgaggt gggcaaggcg
     5521 atcgccgcgg cgctctagag ccccgaatca ctcctcggca aagagttcct ctccgcgagc
     5581 gcgcagcagg gcgagcatct tcttcgccgc tggcgaaagg tatccgtcgc gccggtagac
     5641 aacgtcgaag cgccggggca tcgtcgtttc cctcacgcgc acttccgcga gcgtcgcgcc
     5701 cgcgtcgatt cggctcgtgt gccgtcgcga cgtgaacgtg aggagctccg tcctgcggat
     5761 gagcgtcggc atcatgagga tgaggttcgt ctcgatctgc acgtcgggac cgggcaggcc
     5821 ccggctgtgg aaggcgcgct ccagccattg gcgcgtgccg acggaagtcg gggcgagaac
     5881 ccagcgatag gcgagcagat cctggatcct cgccttccgg cgcaggaccg ggtggctgcg
     5941 gctcgcgatg acgacggctt cgtcgcgaag gatggggtgg atcgcgaggt cggccgcagc
     6001 cttcgtgccg gtgctcacca tgaggtcgat ttcgccggcc tcgagatggc caccgagcac
     6061 gtcgttctgc gcaatcaccg tcttgatcgt gacggcaggc gcctgctgca ggaacacacg
     6121 gcatagcgga ggcatcagga accgcgccag cgtcggcagc acgccgatgc ggacctggcc
     6181 ggccacgccg gagcccaact cgccgatctc cctgacggtg tcctcggccg cgatgcgcag
     6241 ggcccgcgcc cgatgcacca gggcctcgcc tgccgcggtg agccggatcc cgcggcccac
     6301 ccgctcgaag agcgacgttc ccagcgctgc ctcgagccgc cgcacgcttc cggtgagagc
     6361 cggctgcgtg cggaacatct tcgaagccgc cctgcccacg tgcccggtct cggcgatcgc
     6421 ctcgaaatag cgcaggtccc gcagatccaa atgattagac aaaattatcg tttcgaagaa
     6481 ataatgaaag ggacgcgatg aattgtctca ccgatgatgc gtgtgcacca cccgacccgg
     6541 gacacaaccc gggcgggcgc gtccaaccgc gtcatcactg gaggagtcac catgaatcgc
     6601 tgggccaccc gggccgttgc cgcggccgct ttcgtcctta ccgccgggtt ctccggcgcc
     6661 gcgctggcgc aggccggcta ccctgacaag cccgtccgca tgatcgtccc gttccctccg
     6721 ggcggcggca cggacattct ggggcggatc gttggcgaca agctctcctc gacgctcggc
     6781 tggaaggtcg tgatcgagaa caaggccggc gccggcggca ccgtcgggct ggatgcggcg
     6841 gcaaaggcga gacccgacgg atacacgatc gtgatggcgc agacgagcaa cctgtcgatc
     6901 gcgccgtcga tcatgtccgg gctgccctac gaccccgtga agaacttcat gcccgttcgc
     6961 ctggtggcct cggtgccgct tgcgatcgtg gtaggcaagg actccccctt caggtcgctg
     7021 gccgacctcg tggccgccgc caaggcgagt ccgggcaagc ttctctttgc ctcgcccggc
     7081 aacgcgaccg tggcccactt ggccggcgaa tacttccaga aggtcgccgg cgtcaagtac
     7141 acgcacgtgc cgtacaaggg caccggccag gcgctgccgg acgtgatcag tgggcgcgcc
     7201 tcgttctacg tcgcgtcgat cgaatcggcc atgccgcagg tgaaagccgg acagttgcgc
     7261 gccatcgcgg tgacttcggt caagcgcgcg ccgcagtggc cggacgttcc cacggtcgcc
     7321 gaatccggct acgcggggtt cgaggccatc acgtggttcg ggatcgccgt tcccg
//
LOCUS       DASUEV010000247         7291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_639881, whole genome shotgun sequence.
ACCESSION   DASUEV010000247 DASUEV010000000
VERSION     DASUEV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639881"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFV55_07930"
     CDS             <1..667
                     /locus_tag="VFV55_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX4944270.1"
                     /translation="IATNLPFDPAKAFAPVAMVGRGPLILAVSPATPVKTPEEFFALA
                     KSKPGKLNYATSGTGSINHFATELLDDAAGIRMTHVPYKGMGPATTDLMAGHVDVLIA
                     SAPSMLAQVKGGKVKGIGVTTSKASSIAPGLPPLAGAGAPGYNVELWWGILAPAGTPK
                     EIVAKLNAEVNRALATEEMKEFLAREGAEASPATPEAFAEVIRGDIERWKKVAKAADI
                     KAD"
     gene            695..1636
                     /locus_tag="VFV55_07935"
     CDS             695..1636
                     /locus_tag="VFV55_07935"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX4944271.1"
                     /translation="MARLDENARGVYLITVTPFTEGGALDLESTDRVIDFYLEKGATG
                     LTVLGVMGEAPKLTADESRRYVKRVLERVGGKVPTIVGVSSPGFAAMGELAANVMDMG
                     AAGVMVGPASSVKTDDQAVSYYEMAAETLGPSVPFCVQDHPLAYGAQLPVPVLLRIFK
                     SIPSAVMLKHEDWPGPGLAKLAAMRAASTRGEIRRISILAGNGGGLFLPEELSRGADG
                     AMTGFSYPEMMAGVVKAHAAGNVERAHDLFDAYLPLARYEQQAALGLAVRKYLLLKRG
                     AIASAAIRKPGPKLSPADIADIDRLIARQDKRLKELG"
     gene            1636..2340
                     /locus_tag="VFV55_07940"
     CDS             1636..2340
                     /locus_tag="VFV55_07940"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEX4944272.1"
                     /translation="MALDPKIIATLSQITTATITTLLLKKGLRNVWMRGTRPLNPGQP
                     RLVGPAFTLRFVPAREDLATPESWSKPISTRAAIEAMPEGCITVVDAMGITDAGIFGD
                     ILCARMKRKGVAALVTDGVLRDVAGVRGTGLPVWCQGAAAPPSVAGLTFVNWGEPIGC
                     GGVAVFPDDVVVVDDDGAVLIPQALVADIVAGAPEQERLEGWIMAEVEKGASLPGLYP
                     PNAENKARYEASKPKP"
     gene            2337..3224
                     /locus_tag="VFV55_07945"
     CDS             2337..3224
                     /locus_tag="VFV55_07945"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX4944273.1"
                     /translation="MRCKLFVPGSRPELFAKAMASDADAISIDLEDAVDESRKDEARE
                     AVAKFLRSLPADTSKTVIVRVNGVGTPHFEADIEAVAGSGLDLVNLPMPEGPDEVRAA
                     SAVIARIEKAKGVARPAGMLANIETPKAFRLAAGIAGADPRVVGLQLGFGDLLEPYGI
                     ERYDPRVVLHFQLGVKLAAAEAGVFAFDSAMADVKNVEFLKQEAETARRLGYMGKSAI
                     HPSQIAVINAVFRPTEAEIAHSLKVVASAKEAAAKGVGAWTVDGKMIDAPFVVRAEAI
                     LRVARKLGLVAESAGTGWE"
     gene            3229..4467
                     /locus_tag="VFV55_07950"
     CDS             3229..4467
                     /locus_tag="VFV55_07950"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX4944274.1"
                     /translation="MAPPLPAHDYDPKAHGTLEGVRVLDLSRLVAGNTLTQVLADFGA
                     EVIKVEPPAGDTLRGWETKGVATNWKLYARNKKSLALDLRKPEARELLLKLVPTAQVF
                     VESFRPGTLEQMGLAPEVLLARNPKLVIVRISGWGQDGPYAKRPGFGTLVEGMSGFAS
                     LNGFADREPVLPPMYLADSVAGLYGAAGVMIALREVEVKGGKGQVIDLPLLDPLFTVL
                     GPHAANYRLTGKVKPRTGSRSTNSGPRNAYRCKDGLYVGLSASTQKMAERVFRSIGRG
                     DLVDDPRYRTNADRVKNAADLDAIIGAFIAQRSQAENVAFFEKAEVTIGPIYDIAQIV
                     QDPHVLARELVADYPDPDMGAYPMHHVVPRMTGTPGSIRAPAPALGEHNRALLAEAGI
                     SEDEYARLLAEGVACESRPG"
     gene            4477..4821
                     /locus_tag="VFV55_07955"
     CDS             4477..4821
                     /locus_tag="VFV55_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEX4944275.1"
                     /translation="MIAGLVQILLFQGLGEIVARFFLPLIPGPVIGLVLLLAWLRIRG
                     QVPDSVDLVATAFSQNLGLLFIPAAVGVVMFWPQIRSHALAVVAALVVSVVATMAVTA
                     LVLRAIAKDAPE"
     gene            4818..5552
                     /locus_tag="VFV55_07960"
     CDS             4818..5552
                     /locus_tag="VFV55_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEX4944276.1"
                     /translation="MTPALPAIDEIWVYLSGSPLLALVLTLSAYQAGVMIYEKAERNP
                     LANPVAIAIAIVATVITLIDMPYAKYFEGAQFVHFLLGTATVALAVPIYKGLGALRGR
                     IFPLLAALLAGGITSIVSSVGVAKLLGADAAIVGGFYAKSVTAPIAMGIAERIHVSHT
                     LTAVFAVSTGILGAVLVRFVLDAVGSKAWWQRGFAVGVAAHGIGTSRAFSVHPEAGTY
                     ASLGMGLHGVLGAILIPAGVAFVQSM"
     gene            5661..6080
                     /locus_tag="VFV55_07965"
     CDS             5661..6080
                     /locus_tag="VFV55_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944277.1"
                     /translation="MSQENVPPIPLKAGLESPVPALVARVVTESPAPVRSKLITCLLS
                     EVSPLAMVALAQGSFGHFLSRSSSNALIVTAEEARRFTESQILELARYVAQACPAAFV
                     QVANLLQAENPMFTRSLAGGLLFLALDAYVKRKRPTT"
     gene            complement(6152..6862)
                     /locus_tag="VFV55_07970"
     CDS             complement(6152..6862)
                     /locus_tag="VFV55_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX4944278.1"
                     /translation="MPRYPARAPVRSSAETGGVAAVDRALLLLCAFRPGDRELTLAAL
                     AGRSHLVKSTALRLLASLIHFNFIRRSEAGLYSLGPEIARLNGLVASSFSLEDEVMPV
                     LVRLVARTKETAAFHVRRDDRRVCLYRVDSPQLLRDHGRVGDIFPLDRGAGGRVLLAF
                     SGARGAIYDRIRRDGYAALVGDRLPEIAGISAPVFGAGGELIGAITLTLPTHRYKEKA
                     IGPVREAAAAVTRKLGGM"
     gene            6981..>7291
                     /locus_tag="VFV55_07975"
     CDS             6981..>7291
                     /locus_tag="VFV55_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX4944279.1"
                     /translation="MKILVSEVGPRDGLQSVRRTMPTEAKHRWIRALAGAGVREIEVA
                     SFVPPKLLPQMADAAEVVREALKIQGISVLALVPNLRGFQDAAAAGAPKVTLTVSASE
                     A"
BASE COUNT         1184 a         2447 c         2524 g         1136 t
ORIGIN      
        1 catcgcgacg aacctgccgt tcgacccggc gaaggcgttc gctcccgtcg cgatggtggg
       61 caggggcccg ttgatcctcg ccgtctcccc tgccacaccg gtgaagactc ccgaggagtt
      121 cttcgccctg gcgaagtcga agccgggcaa gctcaactac gcgacctcgg ggacgggcag
      181 catcaaccat ttcgccacgg aactcctcga tgacgcggcc ggcatccgga tgacgcatgt
      241 cccctacaag ggcatgggcc ccgcgacgac ggacctcatg gcgggccacg tcgacgtgct
      301 gatcgcgagc gcgccttcga tgctggcgca ggtgaagggc ggcaaggtga agggcatcgg
      361 cgtcacaacc agcaaggcct cctccatcgc tcccgggctg ccaccgctcg ccggagccgg
      421 tgcgccgggg tacaacgtgg aactgtggtg gggcatcctc gcgcccgccg gcacgccgaa
      481 ggaaatcgtg gcgaagctca atgccgaggt caacagggcg cttgccacgg aagagatgaa
      541 ggagttcctc gcgcgcgagg gggccgaggc ctcgcctgcc acgccagagg cgttcgcgga
      601 agtgatccgc ggcgacatcg agcgctggaa gaaggtcgcg aaggcggccg acatcaaggc
      661 ggattgaatc actgcaccgg caagggaaaa tcgcatggca aggctggatg aaaacgcaag
      721 gggggtttac ctcatcaccg tgacgccctt cacggaaggc ggcgccctcg acctggagag
      781 caccgaccgc gtgatcgact tctacctcga gaagggcgcg accggcctca cggtgctggg
      841 cgtcatgggc gaggcgccca agcttaccgc cgacgagtcg cgccgctacg tgaagcgcgt
      901 gctcgaacgg gtgggcggca aggtgcccac catcgtcggc gtgtcctcgc cggggttcgc
      961 ggcgatgggc gagctggccg cgaacgtgat ggacatgggt gcggccggcg tgatggtagg
     1021 gccggcctcg tcggtcaaga ccgacgacca ggcggtgagc tactacgaga tggccgcgga
     1081 gacgctcggg ccctccgtgc ccttctgcgt gcaggaccat ccgctcgcct acggggcgca
     1141 actgcccgtg ccggtgctgc tcaggatctt caagtcgatt ccgagcgcgg tgatgctcaa
     1201 gcacgaggac tggcccggtc ccgggctcgc caagctggcg gcgatgcgtg ccgccagcac
     1261 gcggggagag atccgccgca tctccatcct cgcgggcaac ggcggcgggc tcttcctccc
     1321 ggaggaactc tcgcgcggcg cggacggcgc gatgaccggc ttcagctatc cggagatgat
     1381 ggccggcgtg gtgaaggccc acgcggccgg caacgtcgag cgcgcccacg acctgttcga
     1441 tgcgtacctg ccgctcgcgc gctacgagca gcaggcggcg ctgggcctcg cggttcgcaa
     1501 gtacctgctg ctcaagcgcg gcgcgatcgc ctccgccgcc attcgcaagc ccgggccgaa
     1561 gctttcgccc gcggacatcg ccgacatcga tcggctcatc gcacgtcaag acaagagact
     1621 caaggagctc ggctgatggc actcgacccg aagattatcg caaccctgtc gcagatcacc
     1681 acggcgacga tcaccacgct cttgctaaag aaggggttgc gcaacgtctg gatgcgcggc
     1741 acgcggccgc tcaaccccgg ccagccgcgc ctggtgggcc cggccttcac gctgcgcttc
     1801 gttccggcgc gcgaggacct cgcgacgccc gagtcatggt caaagcccat ctcgacccgc
     1861 gccgccatcg aggcgatgcc cgaaggctgc atcaccgtcg tcgatgcgat gggcatcacc
     1921 gacgccggca tcttcggcga catcctgtgc gcgcgcatga agaggaaggg cgtggcggca
     1981 ctggtcaccg atggcgtgct gcgcgacgtg gccggcgtgc gcggcacggg gcttccggtg
     2041 tggtgccagg gcgccgcggc gccgccttcg gtcgccggcc tcaccttcgt gaactggggc
     2101 gagcccatcg gctgcggcgg cgtcgccgtg ttccccgacg atgtggtggt ggtggacgac
     2161 gacggcgcgg tgctcattcc gcaggcgttg gtggccgaca tcgttgccgg cgcgccggag
     2221 caggagcgcc tcgagggctg gatcatggcc gaggtggaga aaggcgcatc gcttcccggc
     2281 ctctatcccc cgaacgcgga aaacaaggcg cgctacgagg catcgaagcc gaagccatga
     2341 ggtgcaagct cttcgtcccc ggttcgcgcc cggaactctt cgccaaggcg atggctagcg
     2401 acgccgatgc gatctccatc gacctggagg acgccgtcga cgaaagccgc aaggacgagg
     2461 ctcgcgaagc ggttgcgaag ttcctgcgct ccctgccggc ggatacgtcg aagaccgtca
     2521 tcgtgcgcgt gaacggcgtc ggcacgccgc acttcgaggc ggacatcgag gcggtggccg
     2581 gttcgggact cgacctcgtc aacctgccca tgcccgaggg gccggatgag gtgcgagcgg
     2641 cttccgcggt catcgcacgc atcgagaagg cgaagggcgt ggcccggccc gccgggatgc
     2701 tcgcgaacat cgagacgccg aaggccttcc gcctcgccgc cggcattgct ggcgcggacc
     2761 cgcgcgtggt cgggctgcag ctcggtttcg gcgacctgct cgaaccctac ggcatcgagc
     2821 gctatgaccc gcgcgtggtg ttgcacttcc agctcggcgt gaagctcgcg gccgcagagg
     2881 cgggcgtgtt cgccttcgac tcggccatgg ccgacgtgaa gaacgtcgag ttcctgaagc
     2941 aggaagcgga aacggcccgc cggctcggct acatgggcaa gtccgccatc catcccagcc
     3001 agatcgccgt gatcaatgcc gtctttcgcc ccaccgaagc ggagatcgcg cattcgctca
     3061 aggtggtggc ctcggcgaag gaggcggctg cgaaaggcgt tggcgcgtgg acggtggacg
     3121 gcaagatgat cgacgcgccg ttcgtggtgc gcgccgaggc catcctccgc gtggcgagga
     3181 aactcgggct tgtcgcggaa agcgccggaa ccgggtggga gtagggccgt ggccccgccc
     3241 cttcccgcgc acgattacga tccgaaggcg catggcacgc tggaaggcgt gcgcgtgctc
     3301 gacctctcgc gccttgtcgc cggcaatacc ctcacgcagg tgctggccga tttcggcgcc
     3361 gaggtgatca aggtcgagcc gccggcgggc gacacgctgc gtgggtggga aacgaagggg
     3421 gtggcgacca actggaaact ctacgcgagg aacaagaaga gcctcgccct cgacctgcgc
     3481 aagcccgagg cccgcgaact gctcctgaag ctggtcccca cggcgcaggt cttcgtcgag
     3541 agcttccgcc ccggcaccct cgagcagatg ggccttgcgc cggaggtgct gctcgcgcgc
     3601 aacccgaagc tcgtcatcgt gcgcatctcc gggtggggcc aggatggccc gtatgcgaag
     3661 cgccccggct tcggcacgct ggtcgagggc atgtccggtt tcgcttccct caacgggttc
     3721 gccgaccgcg agccggtgct gcccccgatg tacctcgccg attcggtagc cgggctctat
     3781 ggcgcggccg gcgtgatgat cgcgctgcgc gaagtggagg tgaagggcgg caaagggcag
     3841 gtgatcgacc tcccgctgct cgatcccctc ttcaccgtgc tggggccgca cgccgccaac
     3901 tatcgcctca ccggcaaggt gaagccgcgc accggaagcc gatcgaccaa ctccggcccg
     3961 cgcaatgcct accgatgcaa ggacgggctc tacgtggggc tctcggcctc gacgcagaag
     4021 atggccgagc gcgtcttccg ttccatcggc cgcggggacc tcgtggacga cccccgctac
     4081 cggacgaatg cggatcgcgt gaagaacgcc gcggatctgg atgcgatcat cggggcgttc
     4141 atcgcgcagc gatcgcaggc ggagaacgtg gccttcttcg agaaagccga ggtcaccatc
     4201 ggcccgatct acgacatcgc gcagatcgtg caggacccgc acgtcctcgc gcgcgagctg
     4261 gtggcggact atcctgatcc cgacatggga gcgtacccga tgcaccacgt cgtgccgcgc
     4321 atgacgggga cgccgggctc gattcgcgcg cctgccccgg cgctgggcga gcacaaccgc
     4381 gcgctgctgg cggaagcggg cataagcgaa gacgaatacg cgcggctgct ggccgaaggc
     4441 gtggcctgcg agtcacgacc cggctagact cgcggcatga tcgccggcct cgtccagatc
     4501 ctcctcttcc agggcctcgg ggaaatcgtc gcccggttct tcctgccgct cattcccggc
     4561 cccgtcatcg ggctggtgct gctgctcgcg tggctgcgca ttcgcgggca ggtgccggat
     4621 tccgtggatc tcgtcgccac ggccttcagc cagaacctcg ggctcctttt catcccggcg
     4681 gcggtcggcg tggtgatgtt ctggccgcag atccgctcgc acgcgctcgc ggtcgtggcc
     4741 gcgctcgtgg tgagcgtcgt cgcgacgatg gcggtgacgg cgctggttct gcgcgccatc
     4801 gcgaaggacg cgccggaatg acgccggcgc tgcccgcgat cgacgagatc tgggtctacc
     4861 tttccggcag cccgctgctc gcgctcgtcc tcacgctctc ggcctaccag gcgggcgtga
     4921 tgatctacga aaaggcggag cgcaatccgc tggccaaccc ggtcgccatc gcgatcgcga
     4981 tcgtggccac ggtgatcacg ctgatcgaca tgccctacgc gaagtacttc gagggcgcgc
     5041 agttcgtgca cttcctgctc ggcaccgcga cggtggcgtt ggcggtgccg atctacaagg
     5101 ggctcggggc gctgcgcggg cgcatctttc ccctgctcgc ggcgctgctc gccggcggca
     5161 tcacgtcgat cgtgagctcc gtgggtgtgg cgaagctgct cggggcggat gccgcgatcg
     5221 tcggcggctt ctatgccaag tcggtgacgg cgcccatcgc gatggggatc gcggagcgca
     5281 ttcatgtctc gcacacgctc accgcggtgt tcgccgtcag caccggcatc ctaggcgcgg
     5341 tgctcgtgcg tttcgtgctg gacgccgtgg gctcgaaggc gtggtggcag cgcggcttcg
     5401 cggtcggcgt ggccgcgcat ggcatcggca cttcgcgcgc cttcagcgtt cacccggagg
     5461 cgggcacgta cgcgagcctc ggcatgggat tgcacggcgt gctcggcgca atcctcattc
     5521 ctgctggtgt tgcgttcgtg cagtcgatgt agccacatgg cgacacttcc ttgatcctgc
     5581 aataaatttt cactgcacac gcgaaccgat cgaacgaagt gccatccaac tcgactgatc
     5641 ccgggaagga gcagtccacc atgagccagg aaaacgtgcc accgatcccc ctgaaagcgg
     5701 ggctcgaatc gccggttccg gccctcgtcg cgcgggtcgt cacggaatcg ccggcgccgg
     5761 tccgctcgaa gctgatcacc tgcctgttga gcgaggtaag cccgcttgcg atggtcgcgc
     5821 tggcacaggg aagcttcggc catttcctgt cccgttcctc gtccaacgcc ttgatcgtca
     5881 cggccgagga agcgcggcga ttcaccgagt cgcagatcct cgaactggcg cgttacgtcg
     5941 cacaggcgtg ccctgcggcg ttcgtgcaag tcgccaatct gctacaagcg gaaaacccga
     6001 tgttcacccg gagcctcgcc ggcggcctgc tgttcctggc gctcgacgcc tacgtgaagc
     6061 ggaaacggcc gacgacctga cgcgcccgaa tgcggagccg ggatgcccgc gaaatccgcc
     6121 cgcctcgttg acttacggtg tctgtcgccg ttcacatccc gccgagcttc ctggtgacgg
     6181 ccgcagccgc ctcgcgcacc gggccgattg ccttttcctt gtagcggtgc gtgggtagcg
     6241 tgagggtgat cgcgccgatc agctcgcctc ccgcgccgaa caccggcgcc gagatgccgg
     6301 caatttccgg caaacggtcg cccacgagag ccgcatatcc gtcgcgacgg atcctgtcgt
     6361 agattgcgcc cctggcgccc gagaaggcga gcagcacgcg gccgccggcg ccgcgatcga
     6421 gcggaaagat atccccgacg cggccgtggt ccctcagcag ttgcggcgaa tcgacgcgat
     6481 acaggcacac acgccggtcg tcgcggcgca cgtggaaggc cgccgtttcc tttgtccgcg
     6541 ccacgagcct aacgagcacc ggcatgacct cgtcttcgag cgagaacgaa gaagccacga
     6601 gcccgttcag ccgggcaatc tcggggccga gggaatagag ccccgcttcc gagcgccgga
     6661 tgaaattgaa atggatgagg gaggcgagga gccgcagcgc ggtgctcttc accaggtgcg
     6721 agcgcccggc cagcgcggcg agggtgagct cgcggtcgcc gggccggaag gcgcaaagca
     6781 ggaggagcgc gcggtcgacc gcggccacgc cgccggtttc ggcggaagaa cgcacggggg
     6841 cgcgggcagg gtatcggggc atccgggtct cccggcgggc ggcttgacaa gggaccggct
     6901 caagcgccac gattctatcc atcaatcagc agttctatca gacgaaatac tatccgacga
     6961 atttccggag agcaagcccc atgaagatcc tggtgagcga agtcggcccc cgcgacggcc
     7021 tgcagagcgt caggcggacg atgcccaccg aggcgaagca ccgctggatc cgcgcgctgg
     7081 ccggcgcggg cgtgcgggaa atcgaggtgg cctcgttcgt tccgcccaag ctcctgccgc
     7141 agatggccga cgcggccgag gtcgtgcgcg aggcgctgaa gattcaggga atctcggttc
     7201 ttgcgctcgt gcccaacctg cgcgggttcc aggacgcggc cgcggccggc gcgccgaaag
     7261 tgacgctcac ggtgtcggca tcggaagcgc a
//
LOCUS       DASUEV010000248         5004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_639968, whole genome shotgun sequence.
ACCESSION   DASUEV010000248 DASUEV010000000
VERSION     DASUEV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639968"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /gene="pgk"
                     /locus_tag="VFV55_07980"
     CDS             complement(<1..571)
                     /gene="pgk"
                     /locus_tag="VFV55_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX4944280.1"
                     /translation="MTVLKLADLDLKGKRVFIRADLNVPQADDGSITDDTRIRASVPG
                     IRMALDKGAAVMVTSHLGRPTEGTLTEADSLAPVAKRLAELLGRDVPLRRDWVDGVDV
                     SPGQVVLLENCRVNKGEKKNDEALARKMAALCDVYANDAFGTAHRAEATTHGIAKFAK
                     VACAGPLLAAELDALGRALASPKRPLVAIV"
     gene            complement(600..1613)
                     /gene="gap"
                     /locus_tag="VFV55_07985"
     CDS             complement(600..1613)
                     /gene="gap"
                     /locus_tag="VFV55_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX4944281.1"
                     /translation="MTIRVAINGYGRIGRNILRAHYEGGKKHDIVIVAVNDLGNAETN
                     AHLTRYDTAHGKFPGTVTVDGDHMVVNGDRIRVLANRDPAQLPWGELGVDVVLECTGY
                     FNSKEKASAHIRGGAKKVILSAPGGKDVDATIVYGVNHGVLKASHTVISNASCTTNCL
                     APLVKPLHEKIGLVTGLMTTVHAYTNDQVLTDVYHEDLRRARSATMSMIPTKTGAAAA
                     VGLVMPELNGKLDGFAIRVPTINVSLVDLSFIAARDTTVDEVNAIMKTASETAPLKGI
                     LNYNKGPLVSVDFNHDPASSTFDATLTKVSGRLVKVSSWYDNEWGFSNRMLDTTVALM
                     AAK"
     gene            complement(1751..3751)
                     /gene="tkt"
                     /locus_tag="VFV55_07990"
     CDS             complement(1751..3751)
                     /gene="tkt"
                     /locus_tag="VFV55_07990"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX4944282.1"
                     /translation="MATTSATPKDLANAIRALAMDAVQKANSGHPGMPMGMAEIAEVL
                     WRRFLRHNPANPAWADRDRFVLSNGHGSMLQYALLHLTGYALTLEDLKAFRQMHSKTP
                     GHPEVGVTPGVETTTGPLGQGLANAVGMALAERLLAAEFNRPGHTIVDHQTYVFVGDG
                     CLMEGISHEACSLAGTLQLGKLIAIYDDNGISIDGKVEGWFRDNTPMRFAAYGWHVIP
                     NVDGHDPRAVEAAIAEARADSSKPTLICCKTVIGKGSPNKQGTDGVHGAALGEKEVAA
                     TREAIGWPYPPFEIPAAIYQAWDQRARGATLESEWNARFDAYAGAHPESAREFRRRMA
                     GDLPPNWGEVVETMIARAVEKGETVATRKASQIAIEAIAPHLPGFLGGSADLTGSNLT
                     NWSGSKAVSAEHAGNYVNFGVREFGMAAIGNGLALHGGFIPYSGTFLTFSDYSRNALR
                     MAALMRLRNIFVFTHDSIGLGEDGPTHQAVEHVSSLRLIPNLDLWRPGDTVETAAAWA
                     AAIERRDGPTAFALSRQNVPFLKRSPEQVAAIRRGGYVVSEAAGTAKAAIIATGSELS
                     LALAAQKTLAEAGIPVRVVSMPCTGVFDRQDPEWRAAVLPKGLPRVSVEAGVTDYWRK
                     YVGLEGAAIGVDRFGESAPAGAVYKHFGVDADHVVAAVKSVL"
     gene            complement(3848..4729)
                     /locus_tag="VFV55_07995"
     CDS             complement(3848..4729)
                     /locus_tag="VFV55_07995"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEX4944283.1"
                     /translation="MSAKHPIIAITGSSGAGTTSVMRTFEQIFRRENVNAAFIEGDSF
                     HRYDRAEMKAKMAEALASGNRNMSHFGPEANLFAELETLFRTYGETGRGKRRKYLHDE
                     GEAAPYKQPPGTFTAWEDIPEGTELLFYEGLHGAVAGDGVDVAKHPDLLIGVVPVINL
                     EWIQKLHRDRSMRGYSTEAVTDTILRRMHDYVHYITPQFSRTHINFQRVPTVDTSNPF
                     IARYIPTPDESFLVIRFANPRGIDFPYLLSMLHDSFMSRANTIVVPGGKMDLAMQTIF
                     TPMIWRLMERSKQAASR"
     gene            complement(4735..>5004)
                     /locus_tag="VFV55_08000"
     CDS             complement(4735..>5004)
                     /locus_tag="VFV55_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX4944284.1"
                     /translation="DSSKPGRLRLLYEANPMAMLVEQAGGAASTGRGRLLEVEPAALH
                     QRVPLILGSREEVERIERYHRDDDAGIEPPGIAPLFHDRSLFTKA"
BASE COUNT          709 a         1595 c         1823 g          877 t
ORIGIN      
        1 cgacgatggc caccagcggg cgcttcgggc tcgcgagcgc gcgcccgagg gcatccagtt
       61 ccgcggcgag cagcggcccc gcgcaagcga ccttggcgaa cttcgcgatg ccgtgcgtgg
      121 tggcttccgc ccggtgcgcc gtgccgaagg cgtcgttggc gtacacgtcg caaagtgcgg
      181 ccatcttgcg cgcgagcgcc tcgtcattct tcttctcgcc cttgttcacg cggcagttct
      241 cgagcagcac cacctggccg gggctcacgt ccaccccgtc cacccagtcg cgccgcagcg
      301 gcacgtcgcg cccgagcagc tcggcgaggc gcttcgccac gggggccagc gagtcggcct
      361 cggtcagcgt gccttccgtg ggccggccaa ggtgggacgt caccatcacc gccgcgccct
      421 tgtcgagcgc catgcggatg cccggcacgg aggcgcggat gcgcgtgtcg tccgtgatgc
      481 tgccatcgtc ggcctgcggc acgttcaggt cggcgcggat gaagacgcgc ttgcccttca
      541 ggtcgaggtc ggcgagcttg agaacggtca tcttggcacc ggctcgttca acgaccacgt
      601 catttcgccg ccatcagggc gacggtcgtg tcgagcatgc ggttggagaa gccccactcg
      661 ttgtcgtacc aggagctcac cttcaccagg cggccggaca ccttcgtgag cgtcgcgtcg
      721 aacgtggagg aggccgggtc gtggttgaag tccacggaga cgaggggacc cttgttgtag
      781 ttgaggatgc ccttgagcgg ggcggtttcg gaggccgtct tcatgatcgc gttcacctcg
      841 tccacggtcg tgtcgcgcgc ggcgatgaag gagaggtcca cgagggagac gttgatggtg
      901 ggcacgcgga tcgcgaagcc gtcgagcttg ccgttcagtt ccggcatcac cagccccacc
      961 gcggcggccg cgccggtctt ggtcgggatc atcgacatgg tggccgagcg ggcgcggcgc
     1021 aggtcctcgt ggtagacgtc ggtgagcacc tggtcgttgg tgtaggcgtg caccgtggtc
     1081 atgaggccgg tcaccaggcc gatcttctcg tgcagcggct tcaccagcgg ggcgaggcag
     1141 ttggtggtgc aggaggcgtt ggagatcacc gtgtgcgagg ccttcagcac gccgtggttc
     1201 acgccgtaca cgatcgtcgc gtcgacgtcc ttgccgccgg gcgcggagag aatgaccttc
     1261 ttcgcgccgc cccggatgtg ggcggaggcc ttttccttgg agttgaagta ccccgtgcac
     1321 tcgagcacga cgtccacgcc cagctcgccc cacggcagtt gcgcggggtc gcggttggcg
     1381 agcacccgga tgcggtcgcc gttgacgacc atgtggtcgc cgtccacggt cacggtgccc
     1441 gggaacttgc catgcgcggt gtcgtagcgc gtgaggtggg cgttggtctc ggcgttgccg
     1501 aggtcgttga ccgcgacgat gacgatgtcg tgtttcttgc cgccctcgta gtgggcgcga
     1561 aggatgttgc ggccgatgcg gccgtagccg tttatggcga cgcggatggt catgttcttg
     1621 ggctccggaa cgggaaagga tgaggcgggg aaacgcacat tttaagccaa tgggctttca
     1681 ccccctttga cgcaggtcta ggattctcct ctgtgttgcc tcggtgtccc ctggatgagg
     1741 acagcctctc tcagagaacg gacttcacgg cggcgacgac gtgatccgca tcgacgccga
     1801 aatgcttgta cacggcgccg gccggggcgg attcgccgaa gcggtcgacg ccgatggccg
     1861 ctccctcgag gcccacgtac ttgcgccagt aatcggtgac gccggcttcc acggacacgc
     1921 gcggcaggcc cttgggcagc accgcggcgc gccattcggg gtcctggcgg tcgaatacgc
     1981 cggtgcaggg catggaaacc acgcgcaccg ggattcccgc ctcggccagc gtcttctgcg
     2041 cggcgagcgc gagcgacaac tccgagccgg tggcgatgat cgccgctttc gcggtgcccg
     2101 cggcctcgct gaccacgtaa ccgccccggc gaatcgcggc gacctgctcg ggcgagcgct
     2161 tgaggaacgg cacgttctgg cgcgacagcg cgaaggcggt cggcccgtcg cggcgctcga
     2221 tggcggcggc ccaggccgcg gcggtctcga ccgtgtcgcc ggggcgccag aggtcgaggt
     2281 tcgggatcag gcgcaggctc gacacgtgct cgaccgcctg gtgcgtgggg ccgtcctcgc
     2341 ccaggccgat ggaatcgtgg gtgaagacga agatgttcct caggcgcatg agcgcggcca
     2401 tgcgcaaggc gttgcgcgag tagtcgctga aggtgaggaa cgtgccggag tacgggatga
     2461 agccgccgtg cagggcgagg ccgttgccga tggcggccat gccgaattca cgcacgccga
     2521 agttcacgta gttgcctgcg tgctcggcgc ttacggcctt cgagcccgac cagttggtga
     2581 ggttcgagcc ggtgaggtcc gcggagccgc ccaggaaccc gggcaggtgc ggcgcgatcg
     2641 cctcgatggc gatctgggat gccttgcgcg tggcgacggt ctcgcccttc tcgacggccc
     2701 tggcgatcat ggtctcgacg acttcgcccc agttgggcgg caggtcgccg gccatgcggc
     2761 ggcggaattc cctggcggac tccgggtgcg cgccggcgta ggcgtcgaag cgggcattcc
     2821 attcgctttc gagcgtcgcg ccgcgcgcgc gctggtccca cgcctggtag atggccgcgg
     2881 ggatctcgaa gggcgggtac ggccagccga tggcctcgcg cgtcgcggcc acttcctttt
     2941 cgcccagggc cgcgccgtgc acgccgtccg tgccctgctt gttgggcgag cccttgccga
     3001 tgaccgtctt gcagcagatg agcgtgggct tcgaggaatc ggcgcgcgcc tccgcgatgg
     3061 ccgcttccac ggccctcggg tcgtgaccgt cgacgttcgg gatcacgtgc cagccgtagg
     3121 cggcgaagcg cattggcgtg ttgtccctga accagccctc gaccttgccg tcgatggaga
     3181 tgccgttgtc gtcgtaaatg gcgatgagct tgccgagctg cagcgtgccg gcgagcgagc
     3241 acgcctcgtg cgagatgccc tccatcaggc agccgtcgcc cacgaagacg taggtctggt
     3301 gatcgacgat cgtgtggccc ggccggttga actcggccgc caggagcctt tccgccagcg
     3361 ccatgccgac ggcgttggcg agtccctggc cgagcgggcc ggtggtcgtc tccacgcccg
     3421 gcgtgacgcc gacctccggg tgccccggcg tcttcgagtg catctgccgg aacgctttca
     3481 ggtcctccag ggtcagggcg tatcccgtga ggtgcagcag cgcgtactgc agcatcgagc
     3541 cgtgcccgtt ggagagcacg aagcggtcgc ggtccgccca cgcagggttg gccgggttgt
     3601 ggcgcaggaa gcggcgccag agcacctcgg cgatctcggc catgcccatg ggcatgcccg
     3661 ggtggccgga attggccttc tggacggcgt ccatcgcgag ggcgcgaatg gcgttggcga
     3721 gatctttggg cgttgcggag gtggtggcca tgcgcgggga gaggagggcg gactgcatga
     3781 ggggaacctt tcgactcgta ttatcgggca tgggtgccac cgccgccaag cttggcggcc
     3841 gggcgggtca tcgggaggcg gcctgcttgc tgcgctccat gagacgccag atcatcggcg
     3901 tgaagatcgt ctgcatggcc aggtccatct tgccgcccgg caccacgatc gtgttggcgc
     3961 gcgacatgaa ggaatcgtgc agcatcgaca gcaggtacgg gaagtcgatg ccgcgcgggt
     4021 tggcgaagcg gatcacgagg aagctctcgt cgggcgtggg gatgtagcgc gcgatgaagg
     4081 ggttcgaggt gtccaccgtc ggcacgcgct ggaagttgat gtgcgtgcgc gagaactgcg
     4141 gcgtgatgta gtgcacgtag tcgtgcatgc gccgcaggat cgtgtcggtg acggcttccg
     4201 tcgagtagcc gcgcatcgag cggtcgcggt gcagcttctg gatccactcg aggttgatca
     4261 ccggcaccac gccgatgagg aggtccggat gcttcgcgac gtccacgccg tcgcccgcca
     4321 cggcgccgtg cagcccctcg tagaagagga gctcggtgcc ttccgggatg tcctcccagg
     4381 ccgtgaaggt gccgggcggc tgcttgtagg gcgcggcctc gccctcgtcg tgcaggtact
     4441 tgcgccgctt gccccgcccg gtctcgccgt aggtccggaa gagcgtctcg agctcggcga
     4501 ataggttcgc ctccgggccg aaatggctca tgttgcggtt gcccgaagcg agcgcctcgg
     4561 ccatcttcgc cttcatctcg gcgcggtcgt agcgatggaa gctgtcgcct tcgatgaagg
     4621 ccgcgttcac gttctcgcgc cggaagatct gctcgaacgt gcgcatgacc gaagtggtcc
     4681 cggcgcccga ggaaccggtg atcgcgatga tgggatgctt ggccgacatt tggttcaggc
     4741 ctttgtgaag agcgagcggt cgtggaacag gggcgcaatg ccgggcggct cgatgcccgc
     4801 gtcgtcatcg cgatgatagc gctcgatgcg ctccacctcc tcgcgcgagc cgaggatcag
     4861 cggcacgcgc tggtgcagcg cggcgggctc cacctcgagc aggcgcccgc ggccggtgga
     4921 agcggcgccg ccggcctgct cgaccagcat cgccatcggg ttcgcttcgt agagcaggcg
     4981 cagccgcccc ggtttcgagg aatc
//
LOCUS       DASUEV010000249         7035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1654421, whole genome shotgun sequence.
ACCESSION   DASUEV010000249 DASUEV010000000
VERSION     DASUEV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654421"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFV55_08005"
     CDS             <1..736
                     /locus_tag="VFV55_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX4944285.1"
                     /translation="GGFALGKAVGDGNNWAHWLLWSHGGKMVDDKGNVVINSPETVAS
                     LEYAKQLYETFVPGTLSWLDPSNNKAFLDGQVSLTGNGISVYYAAKNSEDAKIKAMAA
                     DINHARFPIGPVGKPTELMQVTQMFLFKYSKYPNAAKAFLAFMMEGDQYNPWMQASIG
                     YTSQPLKAFESNPIWTSDPKHTVYRDGAKIMLDHGYSGPLGYASAAAMADYIVLDMIA
                     EAASGSKSPKEAAERAQKRAERYYKV"
     gene            806..1702
                     /locus_tag="VFV55_08010"
     CDS             806..1702
                     /locus_tag="VFV55_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX4944286.1"
                     /translation="MDRLLNNRNFLGLLFMLPAAACLLVFLTYPLGLGTWLGFTDTKI
                     GRDGVWVGLDNFTYLIGDSVAQLSLFNTLFYTVVASVIKFALGLWLAVLLNRNIPLKS
                     FMRAIVLLPFIVPTALSAIAFWWLYDSQFSVISWTLTRMGLIDAYIDFLGDPWNARLS
                     TIAANIWRGVPFVAICLLAGLQTIAPSLYEAAALDGATPWQRFRFVTLPLLTPIIAVV
                     MTFSVLFTFTDFQLIYVLTRGGPLNATHLMATLSFQRAISGGSLGEGAALATLMIPFL
                     LAAILFSYFGLQRRQWQQGGKD"
     gene            1900..2772
                     /locus_tag="VFV55_08015"
     CDS             1900..2772
                     /locus_tag="VFV55_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX4944287.1"
                     /translation="MKTDDTSTQGMTYLESIPSRIVTLYVPLGIFLFVLLFPFYWMVV
                     TSFKPNGELLSHIGNPFWVVEPTLAHFKKLIFDTPYPEWMLNTVIVSVTATFFSLLAS
                     VLAAYAIERLRFRGSKSVGLGIFLAYLVPPSILFIPLAAIVFKLGLFDTRWALILTYP
                     TFLIPFCTWLLMGYFRSIPFELEECALIDGATRLQILVKIVLPLAVPGLISAGIFAFT
                     LSWNEFIYALTFVSSSEVKTVPVGVITELVEGDVYHWGALMAGALLGSLPVAVMYSFF
                     VEYYVSGMTGAVKE"
     gene            2779..3660
                     /gene="ltnD"
                     /locus_tag="VFV55_08020"
     CDS             2779..3660
                     /gene="ltnD"
                     /locus_tag="VFV55_08020"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: protein motif:HMM:NF043037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HEX4944288.1"
                     /translation="MQAGLIGLGAMGRGMAASLRKQGHAVHVCDVRPEAVASFVAEGG
                     TGHASPASLGAACDVVISVVVNAAQTEAVLFGENGAAAAMKRGSVFVMCSTVDPGWSV
                     ALEKRLEAMGIYYLDAPISGGAAKAAAGQITMMTAGRPEAYAKCQAVLDAMAGKVFRL
                     GDRAGPGSTVKIVNQLLAGVHIAAAAEAMALGIRAGVDPKALYEVITASAGNSWMFEN
                     RMPHVLSGDYTPLSAVDIFVKDLGIVLDTARATKFPLPLASTAHQMFMQASSAGHGRE
                     DDAAVIKVFPGIDLPGK"
     gene            3660..4940
                     /gene="otnK"
                     /locus_tag="VFV55_08025"
     CDS             3660..4940
                     /gene="otnK"
                     /locus_tag="VFV55_08025"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: protein motif:HMM:NF043035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX4944289.1"
                     /translation="MSGILLGAIADDFTGATDLANNLVRCGMRVVQTIGVPGGPLGED
                     VDAVVVALKSRTIAVQEAVAQSLAGLAWLRREGVRQVYFKYCSTFDSTPEGNIGPVTE
                     ALMDALGTDFTIACPAFPENRRTVFKGYLFAGEGLLNESGMQNHPLTPMTDANLVRVM
                     QAQCRRKVGLIDYMAVAKGESAITARVAALRKEGVGIAVVDAISDDDLRRLGPALRDF
                     PLVTAGSGVAIGLPANWGLAPTPRAAQLPPATGHRAIVSGSCSVATRGQVEVFKAAGG
                     QARALDAAALASDASVAAQALDWAAGALGRGPVLVYSTAEPEAVKAVQQRFGIERAGS
                     LVEHALATIARGLVERGVRQLVVAGGETSGAVVQALGVERLRIGPQIDPGVPWCAAHS
                     PVANATVHLALKSGNFGAPDFFAKSFATLAGTPA"
     gene            4937..5593
                     /locus_tag="VFV55_08030"
     CDS             4937..5593
                     /locus_tag="VFV55_08030"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX4944290.1"
                     /translation="MNEQELRHDICRVGRSLFERGYAHATAGNISARLDEGYLITPTD
                     ACLGFLDPDRLAKLDAAGNALSGDRPSKTIALHRRIYEADGSARAVIHTHSTHLVALT
                     LAGVREGDDMVPPITPYFVMKVGHVPLIAYHRPGDPDVGERVAQAIGRFAAKGTPIRA
                     VMLERLGPTVWHDSPGAAMAVLEELEETAKLWLLSNREAAPLREDAIDELRRAFGARW
                     "
     gene            5610..6392
                     /gene="otnI"
                     /locus_tag="VFV55_08035"
     CDS             5610..6392
                     /gene="otnI"
                     /locus_tag="VFV55_08035"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF043033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEX4944291.1"
                     /translation="MPRLAANLSMMYNEHPFLDRFAAAAADGFRGVEFLFPYEHKPDE
                     IRSRLKAYGLTQALFNAPPGDWAKGERGLASIPGREDEYKRSIATALEYTRVLGNRRL
                     HVMAGLIAPGEDRARHRETYVKNIAWAAGEAAGDGITIVIEPINTRDIPGFFLTRQDE
                     AHAVCAEAGAANLKVQMDLYHCQIVEGDLAMKIRKYLRNVGHIQVAGVPERNEPDTGE
                     VNYDYLFDLLDHLGYEGWIGCEYRPIAATSDGLAWAKRWLKH"
     gene            6403..>7035
                     /gene="denD"
                     /locus_tag="VFV55_08040"
     CDS             6403..>7035
                     /gene="denD"
                     /locus_tag="VFV55_08040"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: protein motif:HMM:NF043036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX4944292.1"
                     /translation="MKIVITGGAGFLGLRLAKKLLERGTLTGRSGGPEAISQVVLVDV
                     AKADLDDPRVVHLVGDIADPFFIAAAIGNDAASVFHLAAVVSGQAEAEFDVGMRVNLD
                     ATRGILESCRKLPAPPRVVFASSCAVFGGALPPVVPESWNLQPQTSYGTQKVIGELLV
                     NDFSRKGFIDGRALRLPTICVRPGKPNKAASSFASGIIREPLSGVEAICPV"
BASE COUNT         1115 a         2378 c         2347 g         1195 t
ORIGIN      
        1 cggcggcttc gcgctgggca aggcggtggg cgacggcaac aactgggcgc actggctgct
       61 ctggtcgcac ggcggcaaga tggtggacga caagggcaat gtcgtcatca actcgccgga
      121 gaccgtcgcc tcgctcgagt acgcgaagca gctctacgag accttcgtgc cgggcacgct
      181 ctcctggctc gatccgtcga acaacaaggc cttcctcgac gggcaggtct cgctcaccgg
      241 caacggcatc tcggtctact acgccgcgaa gaactcggag gacgccaaga tcaaggcaat
      301 ggcggcggac atcaaccacg cgcgtttccc catcggcccg gtgggcaagc ccacggagct
      361 gatgcaggtc acgcagatgt tcctcttcaa gtactcgaag taccccaacg cggccaaggc
      421 gttcctcgcg ttcatgatgg agggcgacca gtacaacccg tggatgcagg cctccatcgg
      481 ctacacgagc cagcccctca aggccttcga gtcgaacccg atctggactt ccgacccgaa
      541 gcacaccgtt taccgggacg gcgccaagat catgctcgac cacgggtact cggggccgct
      601 gggctacgcg tcggccgcgg cgatggcgga ctacatcgtc ctcgacatga tcgcggaggc
      661 ggcgagcggc tcgaaatcgc cgaaggaagc ggccgagcgc gcgcagaagc gcgccgaacg
      721 ttactacaag gtctagtacc gggtacacga accgggtaca cgaaccgggt acatgtaccc
      781 ggtacatgta cccggtacgg gaaccatgga ccgcctgctc aacaaccgca atttcctcgg
      841 gctgctgttc atgctgcctg cggcggcgtg cctgctcgtc tttctcacgt atccgctggg
      901 actgggcacc tggctcggct tcaccgacac gaagatcggc cgggacgggg tgtgggtggg
      961 gctggacaac ttcacctacc tcatcggcga ctcggtcgcc cagctttcgc tcttcaacac
     1021 gctcttctac acggtcgtcg cgagcgtcat caagttcgcg ctgggcctgt ggctcgcggt
     1081 attgctcaac cgcaacatcc cgctgaagtc gttcatgcgc gcgatcgtgc ttctgccctt
     1141 catcgtgccg acggcgctct cggcgatcgc cttctggtgg ctctacgact cgcagttctc
     1201 ggtgatttcc tggacgctca ccaggatggg cctcatcgac gcgtatatcg acttccttgg
     1261 cgacccgtgg aacgcgcgcc tgtccacgat cgcggccaac atctggcgcg gcgtgccctt
     1321 cgtggccatc tgcctgctcg cgggcctgca gaccatcgcg ccgtcgctct acgaggcggc
     1381 ggccctggat ggcgccacgc cctggcagcg gttccgcttc gtgacgctgc cgctgctgac
     1441 gcccatcatc gcggtggtga tgaccttctc ggttctcttc acgttcaccg acttccagtt
     1501 gatctacgtg ctcacccgcg gcggcccgct caacgcgacg cacctgatgg cgacactttc
     1561 gttccagcgg gcgatctccg gcggctcgct gggcgagggc gcggcgctgg cgacgctcat
     1621 gatcccgttc ctgctggcgg cgatcctgtt cagttacttc ggactgcagc ggcgccagtg
     1681 gcagcagggc ggaaaggatt gagacgttgc ggctggcgga gacgtaaacc ttggaacacg
     1741 gatgaacacg gataatttca tcgggtattt tatttccgga acttgcggag cagtcgccac
     1801 gaagcgcgac aatctcctgg acttgcagtt atccgtgttc atccgctttt atccgtgttc
     1861 atccgtgttc caaaggtttg cgcccgcata actcccccca tgaagacaga cgacacctcc
     1921 acgcaaggca tgacctacct cgagtcgatc ccgagccgga tcgtcacgct gtacgtgccg
     1981 ctcgggatct tcctcttcgt gctcctcttc ccgttctact ggatggtcgt cacctcgttc
     2041 aagcccaacg gggaactgct ttcccacatc ggcaatccct tctgggtggt ggagcccacg
     2101 ctcgcgcact tcaagaagct gatcttcgat acgccctacc cggaatggat gctcaacacg
     2161 gtcatcgtgt cggtgacggc caccttcttt tccctcctgg ccagtgtcct cgcggcctac
     2221 gccatcgagc gcctgcgctt ccggggctcg aagtcggtgg gactcggcat cttcctcgcc
     2281 tacctcgtgc ccccgtcgat cctcttcatc ccgctggcgg ccatcgtctt caagctgggc
     2341 ctcttcgaca cgcgctgggc gctgatcctc acctatccca cgttcctcat cccgttctgc
     2401 acctggctgc tgatgggcta cttccgctcg atcccgttcg agctcgagga gtgcgccctg
     2461 atcgacgggg cgacgcgcct gcagatcctg gtgaagatcg tgctgccgct ggcggttccg
     2521 gggctcatct cggcgggcat cttcgccttc acgctgtcgt ggaacgagtt catctacgcg
     2581 ctcaccttcg tctcctcgtc ggaagtgaag accgtgccgg tgggcgtgat cacggagctc
     2641 gtggaaggcg acgtctacca ctggggcgcg ctcatggcgg gggcgctgct gggctcgctg
     2701 ccggtcgcgg tgatgtactc cttcttcgtg gagtattacg tctccggcat gaccggcgcc
     2761 gtcaaggaat aggaaaccat gcaagccgga ttgatcggcc tgggagcgat gggccgcggc
     2821 atggcggcgt cgctgcgcaa gcagggccat gcggtccacg tctgcgacgt ccggcccgag
     2881 gcggtggcct ccttcgtcgc cgaaggcggc acgggacacg cgagcccggc gagcctcggc
     2941 gcggcttgcg acgtggtgat ctccgtggtc gtgaacgccg cgcagaccga agccgtgctc
     3001 ttcggcgaga acggcgccgc ggccgcgatg aagcgcgggt ccgttttcgt catgtgctcc
     3061 accgtcgatc cgggctggtc ggtcgcgctc gagaagcgcc tcgaggcgat gggcatctac
     3121 tatctcgacg cgcccatctc cggcggggcg gccaaggcgg cggcggggca gatcacgatg
     3181 atgaccgcgg gcaggcccga ggcgtacgcg aagtgccagg ccgtgctcga tgcgatggcc
     3241 ggcaaggtat tcaggctcgg cgaccgcgca gggccgggca gcacggtgaa gatcgtgaac
     3301 cagctccttg cgggcgtgca catcgcggcg gcggcggagg cgatggcgct cggcattcgc
     3361 gccggcgtcg acccgaaggc gctctatgaa gtcatcacgg cgagcgccgg caacagctgg
     3421 atgttcgaga accgcatgcc gcacgtgctg tccggcgact acacgccgct ttccgccgtg
     3481 gacatcttcg tgaaggacct ggggatcgtc ctcgacacgg cgcgcgcgac gaagtttccg
     3541 ctgccgctcg cctcgaccgc ccaccagatg ttcatgcagg cctcttccgc cgggcacggg
     3601 cgcgaggacg acgccgcggt gatcaaggtc tttcccggga tcgacctgcc gggaaaatga
     3661 tgagcggcat cctcctcggc gccatcgcgg acgacttcac cggggcgacc gaccttgcca
     3721 acaacctcgt gcgctgcggc atgcgcgtgg tgcagacgat cggagttccg ggcgggccgc
     3781 tgggcgagga cgtcgatgcg gtcgtggtcg cgctcaagtc gcgcacgatt gccgtgcagg
     3841 aagccgtcgc gcagtcgctt gcagggctcg cctggctgcg gcgcgagggc gtgcggcagg
     3901 tgtacttcaa gtactgctcc accttcgact cgacgcccga aggcaacatc ggcccggtga
     3961 ccgaggcgct gatggacgcc ctcggcacgg acttcaccat cgcctgcccg gcctttccgg
     4021 agaacaggcg cacggttttc aagggttacc tgttcgcggg cgaggggctc ctcaacgagt
     4081 ccggcatgca gaaccacccg ctgacgccca tgacggacgc aaacctcgtg cgcgtgatgc
     4141 aggcgcagtg ccgcaggaag gtgggcctca tcgactacat ggcggtcgcg aagggtgaga
     4201 gcgccatcac ggcccgtgtc gccgcgctgc gcaaggaagg cgtcggcatt gccgtcgtgg
     4261 acgccatttc ggacgacgac ctgcgccgcc tcgggcctgc gctcagggat tttccgctgg
     4321 tgaccgcggg ctccggcgtg gccatcggac tgcccgccaa ttggggtctc gcgcccacgc
     4381 cgcgcgcggc gcaattgccg ccggccaccg ggcaccgcgc catcgtgtcg ggaagctgct
     4441 cggtcgccac gcgcgggcag gtcgaggtgt tcaaggcggc cggcgggcag gcgagggcgc
     4501 tcgatgccgc ggcgctcgcg agcgatgcat ccgtcgcggc gcaggcgctg gactgggcgg
     4561 ccggggcgct cggccgcgga ccggtcctcg tctactcgac cgcggaaccg gaagcggtca
     4621 aggccgtgca gcagcgcttc ggcatcgaaa gggcaggcag cctggtcgag cacgcgctgg
     4681 cgacgattgc gcgcggcctc gtcgagcgag gcgtgcgcca gctcgtcgtg gcgggcgggg
     4741 agacttcggg cgcggtcgtg caggcgctcg gcgtcgagcg gctgcgcatc gggccgcaga
     4801 tcgacccggg cgtgccctgg tgcgcggcgc actcgcccgt ggcgaacgcg accgttcacc
     4861 tggcgctcaa gagcgggaac ttcggcgcgc ccgatttctt cgcgaagtct ttcgcaaccc
     4921 ttgcgggaac gcctgcatga acgagcagga gttgcgccac gacatctgcc gcgtcggccg
     4981 cagcctgttc gagcggggct acgcgcatgc caccgcgggc aacatcagcg cgcgcctcga
     5041 cgagggctac ctcatcacgc ccaccgacgc gtgcctcgga ttcctcgacc ccgaccggct
     5101 tgcgaagctc gacgcagccg gcaacgcctt gtcgggcgac cgcccgagca agacgatcgc
     5161 gctccaccga cgcatctacg aggccgacgg gagcgcgcgc gccgtgatcc acacccactc
     5221 gacgcacctt gtcgccctga cgctcgccgg cgttcgcgaa ggggacgaca tggtgccccc
     5281 gatcacgcca tacttcgtga tgaaggtggg gcacgtaccg ctcatcgcct accaccgccc
     5341 cggcgatccg gacgtgggcg agcgggtcgc gcaggcgatc gggcggttcg cggcgaaggg
     5401 gacccccatc cgtgccgtga tgctcgaacg cctcggcccc accgtgtggc acgattcacc
     5461 gggcgcggcg atggccgtgc tcgaggaact ggaggagacc gcgaagctgt ggctcctctc
     5521 caaccgcgag gcggcgccgc ttcgcgagga tgcgatcgac gaacttcgcc gcgccttcgg
     5581 cgcgcgctgg tagacccgag gagacagcca tgccgcgcct ggccgcgaat ctttcgatga
     5641 tgtacaacga gcatccgttc ctggaccgct tcgccgccgc cgcggcggac ggcttccgcg
     5701 gggtggagtt cctgttcccg tacgagcaca agcccgacga gatccgttcg cggctgaagg
     5761 cctacggcct cacccaggcg ctcttcaacg cgccgcccgg ggactgggcc aagggggaac
     5821 gcggcctggc ctcgatcccc ggccgcgagg acgaatacaa gcgctccatc gccacggcgc
     5881 tcgagtacac gcgcgtcctc ggcaaccggc gcctgcacgt gatggcgggc ctcatcgcgc
     5941 cgggcgagga tcgcgcccgc catcgcgaaa cctacgtgaa gaacatcgcg tgggcggcgg
     6001 gggaggcggc gggcgacggc atcaccatcg tgatcgagcc gatcaatacg cgcgacatcc
     6061 ccggcttctt cctcacccgg caggacgagg cgcatgccgt gtgcgcggaa gcgggtgcgg
     6121 cgaacctcaa ggtgcagatg gacctctacc actgccagat cgtggaaggc gacctggcga
     6181 tgaagatccg caagtacctg cgcaacgtgg ggcacatcca ggtcgcgggc gtgccggagc
     6241 gcaacgagcc ggacacgggg gaggtgaact acgactacct cttcgacctg ctcgaccacc
     6301 tgggctacga aggctggatc ggctgcgaat accggccgat agccgccacc tcggacgggc
     6361 tggcctgggc gaagcggtgg ctgaagcact aggaaggcgg ccatgaagat cgtcatcacc
     6421 ggaggcgcgg gctttctcgg cctgcgcctt gcgaagaagc tcctcgagcg cggcacgctc
     6481 acgggtcgtt cgggcgggcc cgaggcgatc tcgcaggtgg tcctggtcga cgtcgcgaag
     6541 gcggacctgg acgatccgcg cgtggtacac ctcgtgggcg acatcgccga tcctttcttc
     6601 atcgcggccg cgatcggaaa tgacgccgcg tcggtgttcc acctggccgc ggtggtgagc
     6661 gggcaggcgg aggctgaatt cgacgtcggc atgcgggtga acctggatgc gacgcgcggc
     6721 atcctggaat cctgccgcaa gctaccggca ccgccgcgcg tggtgttcgc gagcagctgc
     6781 gcggtattcg gcggcgcctt gccgcccgtg gtgccggaat cgtggaacct gcaaccgcag
     6841 acctcctatg gcacgcagaa ggtgatcggc gagctcctcg tgaacgactt ttcgcgcaag
     6901 ggcttcatcg acgggcgggc gctgcggctc cccacgatct gcgtgcggcc cggcaagccc
     6961 aacaaggcgg cctcctcgtt cgccagcggc atcattcgcg agccgctttc cggcgtcgag
     7021 gcgatctgcc ccgtt
//
LOCUS       DASUEV010000250         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_402121, whole genome shotgun sequence.
ACCESSION   DASUEV010000250 DASUEV010000000
VERSION     DASUEV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402121"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFV55_08045"
     CDS             <1..313
                     /locus_tag="VFV55_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX4944293.1"
                     /translation="LPPLSLLGTLAFLGSAFPATATTPAERVICTAEPRSRWMPEEEA
                     RKIFGAEKYALVKFKVSRTNCYEFYAVGRNGGVVEAYYHPVTGRLVKETRVGEDRRAG
                     P"
     gene            387..1595
                     /gene="hemW"
                     /locus_tag="VFV55_08050"
     CDS             387..1595
                     /gene="hemW"
                     /locus_tag="VFV55_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX4944294.1"
                     /translation="MQPVTFAPRTGGAVRLAALPPLTLYIHVPWCLKKCPYCDFNSHE
                     AGGELPEARYVDAVIADLEASVPRVWSRRVHAIFIGGGTPSLFSAPGIDRLLTAARTL
                     LPVEADAEVTLEANPGTFEQAKFRDFRSAGVNRLSLGVQSFDERRLKAIGRVHDADEA
                     RRAAEFAVATFGNVNLDLMYALPDQSVDEAVADIRTAIAFGPPHLSAYHLTLEPNTYF
                     HRYPPPLPDEDSAAEMQEAIETELGAAGYENYETSAFARPGRRCRHNLNYWRFGDYLG
                     VGAGAHSKLSFHDRITREVRFRQPRAYQDQALAGNAVQEAHEVGSGDIPVEFLMNALR
                     LAEGFPVAMYAERTGLSLAGIEGPLARAQAAGLLERDVFSIRPTPKGRRFLNDLLGLF
                     LPEAKSPRNV"
     gene            1675..>2760
                     /locus_tag="VFV55_08055"
     CDS             1675..>2760
                     /locus_tag="VFV55_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4944295.1"
                     /translation="MPKYTESIHTLALVGHGGAGKTTLAEALLWKSGAIATPGTVEKG
                     TTVCDFDAMEKEYGHSLSSSLVNFDYGDMHIHLIDTPGMPDFAGQSIAALAAVETAVV
                     VINAQNGIELNTTRMMKWAKTRGLCRMVVVNKIDADNVDLPGLLRKIQDHFGKECLPI
                     NLPAHDRKDVVDCFFTPDGDADFVGVKAAHTAIVEQVVEVDEDLMTLYLEQGEELEPE
                     QLHAPFEKALREGHLVPVCFTSAKTGAGIPQFLEILSRLAPNATEGNPPPFVKSDTTE
                     GEEFHAVPDRTKHVLAHVFKIVMDPYVGKVAFFRVHQGTITRDSQLYIGDGKRPFKVG
                     HLYSIQGKDYVEVNELFPGDLGAVAKVD"
BASE COUNT          467 a         1015 c          876 g          402 t
ORIGIN      
        1 cctccccccg ctttccctgc tcggcacgct ggcgttcctt ggcagcgcat ttcccgcgac
       61 cgccaccacc ccggccgagc gcgtgatctg caccgccgag ccccgctcgc gctggatgcc
      121 cgaggaggag gcgcgaaaga tcttcggcgc cgagaagtac gcgctcgtga agttcaaggt
      181 ctcgcgcacc aactgctacg agttctatgc cgtcggccgc aacggcggcg tcgtcgaggc
      241 ctactaccac ccggtcaccg gacgcctcgt gaaggaaacc cgcgtcggcg aggatcggcg
      301 cgccggaccg taggctgcgc gccggcggtg gccggcccca taatgcgggg gtgcgacctt
      361 cacaccccac ccgacggtag cgccccatgc agccggtcac cttcgcgcca cgcacgggcg
      421 gcgcggtacg cctcgcggcc ctgccgccgc tcacgctcta catccacgtt ccgtggtgcc
      481 tgaagaagtg cccctactgc gacttcaatt cgcacgaggc gggcggggaa ttgcccgagg
      541 cgcgctatgt cgacgcggtc atcgccgacc tcgaggcgag cgtgcccagg gtctggagcc
      601 ggcgcgtgca cgcgatcttc atcggcggcg gcacgcccag cctcttttcc gcgcccggca
      661 tcgaccgcct tctcacggcg gcacgaacgc tgctgcccgt ggaagccgac gccgaggtca
      721 cgctggaagc caatcccggc accttcgagc aggcgaagtt tcgcgacttc cgcagcgcgg
      781 gcgtgaaccg gctctcgctg ggcgtccaga gcttcgatga acggcgcctg aaggccatcg
      841 gccgcgtgca cgacgcggac gaggcaaggc gcgcggccga gttcgccgtc gccaccttcg
      901 gcaacgtgaa cctcgacctg atgtacgcgc tgccggacca gtcggtggac gaggcggtcg
      961 ccgacatccg cacggcgatc gccttcggtc cgccgcacct ctcggcctac cacctcacgc
     1021 tcgagcccaa cacgtatttc caccgctacc cgccgcccct gcccgacgag gactcggctg
     1081 ccgaaatgca ggaggcgatc gagacggaac tcggggcagc gggctacgaa aactacgaga
     1141 cctccgcgtt cgccaggccc ggacggcgct gccgccacaa cctcaactac tggcgcttcg
     1201 gcgactacct cggcgtgggc gcgggcgcgc attccaagct ctccttccac gaccgcatca
     1261 cgcgcgaggt ccgcttccgg cagccgaggg cctaccagga ccaggcgctc gccggcaacg
     1321 ccgtgcagga ggcgcacgaa gtgggcagcg gcgacatccc cgtggaattc ctgatgaacg
     1381 cattgcgcct cgcggagggc tttcccgtcg cgatgtacgc cgagcgcacc gggctttcgc
     1441 ttgcggggat cgaaggtccg ctcgcccgcg cccaggccgc cggcctcctc gagcgcgacg
     1501 ttttctccat ccgccccacg ccgaaggggc gccggttcct caacgacctg ctgggtttgt
     1561 tcctgccgga ggcgaaatcg ccccggaacg tgtaaattct ggggtcggaa gtttccctac
     1621 ccccgcgacc acccgctccc ccgaaaatcc cgctccccca agaggaaccg catcgtgccg
     1681 aagtacaccg aatccattca caccctcgcg ctcgtcggcc acggcggcgc cggcaagacg
     1741 acgctggccg aggccctgct ctggaagtcc ggcgccatcg ccaccccggg caccgtcgag
     1801 aagggcacga cggtctgcga cttcgacgcc atggagaagg agtacgggca ctcgctctcg
     1861 tcctcgctgg tgaacttcga ctacggggac atgcacatcc acctcatcga cacgcccggc
     1921 atgcccgact tcgccggcca gtcgatcgcc gcgctggccg ccgtggagac cgcggtcgtc
     1981 gtgatcaacg cgcagaacgg catcgagctc aacaccacgc gcatgatgaa gtgggcgaag
     2041 acgcgcggcc tgtgccgcat ggtcgtggtg aacaagatcg acgcggacaa cgtggacctg
     2101 cccgggctcc tgcgcaagat ccaggaccac ttcggcaagg aatgcctgcc catcaacctg
     2161 ccggcccacg accgcaagga cgtggtcgac tgcttcttca cccccgacgg cgacgccgac
     2221 ttcgtgggcg tgaaggccgc gcacacggcc atcgtcgagc aggtggtcga ggtggacgag
     2281 gacctgatga ccctctacct cgagcagggc gaggagctgg agccggagca gctgcacgcg
     2341 ccgttcgaga aggcgctgcg cgaggggcac ctggtccccg tctgcttcac ctccgcgaag
     2401 acgggcgcgg gcatcccgca gttcctcgag atcctctcgc gcctcgcgcc caacgcgacg
     2461 gagggcaacc cgccgccgtt cgtgaagagc gacaccaccg agggcgagga attccacgcc
     2521 gtgcccgacc gcaccaagca cgtgctcgcg cacgtgttca agatcgtgat ggacccctac
     2581 gtgggcaagg tggccttctt ccgcgtccac cagggcacga tcaccaggga ctcgcagctc
     2641 tacatcggcg acggcaagcg cccgttcaag gtagggcacc tctattccat ccagggcaag
     2701 gactacgtcg aggtgaacga gctctttccc ggcgacctgg gcgccgtggc caaggtggac
//
LOCUS       DASUEV010000251        13408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_295529, whole genome shotgun sequence.
ACCESSION   DASUEV010000251 DASUEV010000000
VERSION     DASUEV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295529"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFV55_08060"
     CDS             complement(<1..824)
                     /locus_tag="VFV55_08060"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX4944296.1"
                     /translation="MTQQPLLIGCGAGFSGDRTDAADPVVTTMAERGGPACLMLETLA
                     ERTLALAQLRRREDPEAGYEPMLDALVAPILAECLGSGIRIVSNFGAANPEGAAMRID
                     EIARAQGFPNPRIAVVTGDDLSAPAYHQLLAQHLGDAHRPVSANAYLGAEVIAAAWDA
                     GAEVIVTGRVADPSLAVGPAMAHFGWDASDWDRLGRATMAGHLLECGSQVTGGYFADP
                     GFKDVPDLASVGYPVAEIAADGSCVIGKAAQTGGCVTLATVKEQLLYEVHDPAAYLT"
     gene            886..1791
                     /locus_tag="VFV55_08065"
     CDS             886..1791
                     /locus_tag="VFV55_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4944297.1"
                     /translation="MSQLNMSTRHLRAFLALAELRSFTRAANRCHLSQPAFSALVRSL
                     EDSLGARLFDRDTRNVELTAEGRLLEEPARRLVADFEGLVTDIGDRVARRKGRVSVAA
                     LPSLAAGWLPGIFAEFRRANPGVDLALHDTLSDQCLDLVRAGRADFAIAATGPESPEW
                     STEVLCRDRFHLVCRRDHPLARKALLHVKDLAAYPFVHFSRASSVRQHLEAALHPQQM
                     STVLEVEHLTTVMGMVEEGVGITVVPALTLFEFERGSLMHRPLAVANLKRTIYIVRRA
                     DRSLSPAAQALYDLMIERKPAPRRT"
     gene            1890..2363
                     /locus_tag="VFV55_08070"
     CDS             1890..2363
                     /locus_tag="VFV55_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944298.1"
                     /translation="MKIIATLVATGAFVMSAALLSPLAVAVDTHDHGHAAGAAKLQLD
                     HGKKWATDAPLRQGMTAIREAVHGAPAPLHKATAKPEAYAEVGNRVEAEVGRIVKECK
                     LPPEADAQLHLVIADVIAGADAMKGARDAKAGRAGLVKVDSALKNYGTYFDHPGW"
     gene            2429..2920
                     /locus_tag="VFV55_08075"
     CDS             2429..2920
                     /locus_tag="VFV55_08075"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX4944299.1"
                     /translation="MSDRHDAPTIPPGFRAIAIGGPFMQTVGPLYGRWDGERVRLGLR
                     VEERHANIAKACHGGMLTSFADMQMAVATHYQWPEIAGHSFPTINLTTDFVAPVPLGE
                     WLEGSADVIRATKSLVFIQGTAAVDGATVLRFSGVYKIGPKRDYANPRDPFGLLPLSS
                     SGR"
     gene            2926..3756
                     /locus_tag="VFV55_08080"
     CDS             2926..3756
                     /locus_tag="VFV55_08080"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX4944300.1"
                     /translation="MDQPVVIAAAITGSVPRKKDTPAIPVIPSEQIESTHQAFEAGAS
                     LVHIHVRNADESSGSDPAKFAEVQEGVKKHCPGMIIQFSTGGRGREASQRGAMLHLKP
                     DMASLATGSVNFPTMIYENPPDFIDKLAGRMLELDIKPECEIFDLAMLYNAADLVKRQ
                     LLNAPPHVQFVFGIKGAMPARRSILEFLVSELNIVLPGATWTAAGIARHQFEVAQWAL
                     EMGGHCRTGLEDNIRYDETRLARSNAELVEKLVAECARRGRRVATPAEARQILGLRPA
                     "
     gene            complement(3774..5195)
                     /locus_tag="VFV55_08085"
     CDS             complement(3774..5195)
                     /locus_tag="VFV55_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX4944301.1"
                     /translation="MSHTRREFLQSAGAALGALAIPSTTLLAAPPRLELTAGVFRQKI
                     RPSGQETEVWGFNGSVPGPLLRFRQGDPVDIQVTNTLPQATTVHWHGVRVPNAMDGVP
                     FVTQVPIAKGGTFDYRYRVEDAGTFWYHPHQSSFEQVPRGLYGMLVVEERKPLPVDRE
                     ERWVLSDYKLGDDNLAVEDFGKMLDFGSEGRFGNVVMLNGTAAGPGRRLELRPGERVR
                     LRLLNAASARAFLLEFTGHSPWIVSYDGQAIAPQPVPDGRLLLGAGQRTDLVIDGVGS
                     AGQSFAVTEHRRGGFEIARIAYDGKPVRAKVLGRPAAIVPNRHTEPDVGKATDHYLVF
                     EGGMKGAPAIGKVDGKALKVEEIMEKHGLAWTMNYTAQHELAMMHEPLFRMRKDEHVT
                     LKMINNTDYEHPMHLHGHFFRVLALNDVPTKLREWRDTVMIDPRGSCDIAFVAENVGE
                     WMLHCHILDHAAGGMMGTVVVEG"
     gene            complement(5381..8101)
                     /locus_tag="VFV55_08090"
     CDS             complement(5381..8101)
                     /locus_tag="VFV55_08090"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEX4944302.1"
                     /translation="MRTTAAQCIGRLATLIRPNATVLVAGLGVAFAPVATRADSVSGT
                     DTSAGNAMSQGIAAGPPRVEADIDPKRSPIGILFGPRDLLSEPRKTVQDGWLYRAEVE
                     FGGLDASGDHRNPLFGRYRDVDSGLVLHHLAVDAEKPDTATFVRANAGSVARDDQFHS
                     IAFGRHNAWKVRAFFNETPSVATNTFRSLWNGIGTDRQTLATLTPGGQATPDASLAAL
                     RNAIAAAPETELAITRKKGGVRVDVTLSETWKLYADYTAEKKRGAAPYAATFGAGDGG
                     GNFEFAESVDSTTHDFQGGISYFDRVNSFNLTLQGSLYRNAIDTLTVENPLAIAVNTV
                     AGVPAGTFMSARFDMYPDNNHYKAKAEYARNLPSLMNGRFSAVVAATRSQQDDALIAP
                     TALPLTGGTINGISAANRWNTLDSLSRRTSGAEIETTLVNLGLSLNPAPSATLRANLR
                     RYETDNSTDFLACNPLTGQWGRLLNDGSGGAFVNTPAYLAAGCDLAAVRALGVSPSAG
                     NINIRAVPFQYTQTNYTLAADWRATARSNVTGSFEREEYDREHRERDKTREDKVKVGF
                     TNRGFDNVTLLFSLENARRRGSAYVANPYEEFLSASMGPLPTGATGNFATWIHAIDSF
                     RRFDLADRDSRTANGRINWAVMPDVDVGLSAQFRDTRYPDAEFGRNGTNRDASVSVEA
                     NWQASAEFALYGYYTWQSRKMHQTGLQANSCVAGVTYFFYSNGEVNTTGIAPAGATRI
                     GSTQVTAANAIATCAAAGPLSPLFPAGNSWEQFQEQRNHAASIGLRRDFGLAKLDASY
                     TYASGRSTSTYLYNAAALSISAAEVALVGSGFPEARLVQNTVEANLTYPIGRSMSARL
                     YYRYERGRISDWHYDGVAGNPAPDAFAAYLDGGPGNYSASTVGLFIRVAF"
     gene            complement(8120..9052)
                     /locus_tag="VFV55_08095"
     CDS             complement(8120..9052)
                     /locus_tag="VFV55_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEX4944303.1"
                     /translation="MRRWTGCAGLLLLAASCLQGVVARAQEAEYVGEARCITCHEANN
                     RHFTETPHARAFRGNPKNDLERRVCEACHGPGSKHVANAADKSAPIRFTRQSATPVET
                     MNAQCLQCHGGGQRQHWPGSAHAQNLVACSDCHAPMERRSTDGLLRRPTINETCLACH
                     AQQRAQFQKRSHMPVLEGKMSCVDCHNPHGSITRPLLRQDSVNETCVACHAEKRGPFL
                     WEHAPVRENCANCHDPHGSNHERLLVSARPFLCQQCHNLPVGHSSAFYRGDQTTAAAL
                     AGGTPSPRVIGRSCQNCHSAIHGSNHPSGARLQR"
     gene            9227..10465
                     /locus_tag="VFV55_08100"
     CDS             9227..10465
                     /locus_tag="VFV55_08100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX4944304.1"
                     /translation="MKSLNRLRAIALLSAALAAPAWSQAPSVTPYYPDAQWQRKSPAE
                     AGIDATKLKDAIDLAIASESRNPRDLTLNHYRTFGREPFGIAIGPIRDRGDMTGVIVH
                     RGYIVAEWGEPSRVDMTHSVTKSLLSSVVGVAFDRGMIRSLEDPVRDYVAPTLVYRPP
                     AAASDKAEQLGRSDLLWLFETPHNRTITWDHLLRQTSDWEGTLWGKPDWADRPGDTPA
                     EWTTRARNTPGTAYKYNDVRVNVLALAALNVWRRPLPQVAKEHIMDPIGASNTWRWFG
                     YENSFVVLDGAIVQSVTGGGHWGGGLFINAYDMARFGYLTLRKGRWKDRQVLSTQWVD
                     RALTPTPVQPGYGFMNWYNNRERKLLPSAPASAFAHIGNGTNIIYVDPEHDLVAVLRW
                     IQSPAIDAVVQRLIEALPAR"
     gene            complement(10480..12309)
                     /gene="glmS"
                     /locus_tag="VFV55_08105"
     CDS             complement(10480..12309)
                     /gene="glmS"
                     /locus_tag="VFV55_08105"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX4944305.1"
                     /translation="MCGIVGAIANRNVVPILTEGLKRLEYRGYDSAGIAILDGGIRRV
                     RRVGRVTEMEGAAKAENLSGIVGIAHTRWATHGGVTEPNAHPHISSNEIAVVHNGIIE
                     NHEEQRARLKVLGYAFESQTDTEVIAHLVHHYFKATGDLFAATQKAAADLRGAYAIAV
                     LALKDPETITGARMGCPLVVGLGESENFLASDVSAIVSETRRVVYLEDGDVVAVHRSG
                     VSVVDIAGKPVNRPERLSTLSADAVDLGPYSHYMQKEIHEQPRAVADTLEAVIDGGVD
                     VDGLFGTGAAAAFRDTDALLILASGTSFYAGLVARFWLESIAKVPCNVELGHEYRYRD
                     SIPNPRQLVVTISQSGETLDTMEALKRARELGQANTLSICNVHESAIPRASRFVFYTR
                     AGMEIGVASTKAFTTQLVALFTLTLALAKAKGRLDPNSEAAAIAQLRFVPGSVQHALN
                     LEPQVQVWAERFAHKEHALFLGRGIHYPVALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALVDSEMPVVVIAPNDSLLEKVKSNMQEVRARGGELYVFADLDSHFSESAGVHVIRTP
                     RHVGLLSPIVHTIPVQLLAYHTALRRGTDVDKPRNLAKSVTVE"
     gene            complement(12358..>13408)
                     /locus_tag="VFV55_08110"
     CDS             complement(12358..>13408)
                     /locus_tag="VFV55_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX4944306.1"
                     /translation="AGLAFHLSPWGAKASLVMLDAGFGILRAIAPGKSPDDILIVGLH
                     DAATVAEVGSAGPGLRQLPDVLVRIARGRPRAIALHARLPRASQEAAAPGWDDALATA
                     LSVAQAAAPLAVGMSVDSRREVVPIHDALLRGVDTQALVFTLLPRDEDGVVRQIVLAL
                     PTQQGKWPTSAGWLCAVLGGKCEPGLIDYALGPAYRYLPAQRLLDIRDDTALARLFRG
                     RVVFVAPVTGPDDRVPQPVSLAGWEPPSREPAAVLAHAQTVRTLLHGNPIHEAPLPAM
                     LLATAAAALVALIAAPVAPAVGALAAGGCIAGALFALRLGWYAPPAAVLATLVAAVVA
                     SRILGRMSHPARGVT"
BASE COUNT         2109 a         4495 c         4622 g         2182 t
ORIGIN      
        1 gtgagatacg ccgccggatc gtgcacttcg tagaggagct gttccttcac ggtggcgagc
       61 gtcacgcaac cgccggtttg cgcggccttg ccgatcacgc agcttccatc ggccgcaatc
      121 tccgccaccg gatagcccac actcgcgaga tccggcacgt ccttgaaccc gggatcggcg
      181 aagtagcccc cggtcacctg cgaaccgcac tcgagaaggt ggccggccat cgtggcgcga
      241 cccaggcggt cccagtcgct cgcatcccag ccgaagtgcg ccatcgccgg cccgacggcg
      301 agcgaggggt ccgccacgcg cccggtgacg atgacctccg cgccggcatc ccatgccgcg
      361 gcaatcacct ccgcgccaag gtaggcgttt gcgctcacgg gccggtgggc gtcgccgagg
      421 tgttgcgcga gcaactggtg gtaggccggc gccgagagat cgtcgccggt cacgacggcg
      481 atccgggggt tgggaaatcc ctgcgcgcgg gcaatctcgt cgatgcgcat cgccgcgccc
      541 tcgggattgg ccgccccgaa attggacacg atgcggatgc cgcttccgag gcactcggca
      601 aggatcggcg ccacgagcgc gtcgagcatc ggctcgtatc cggcctcggg atcctcccgg
      661 cggcgaagct gcgccagcgc gagcgttcgc tccgcaagcg tctcgagcat gaggcaggcg
      721 gggccgccgc gctcggccat cgtggtgacg accgggtccg cggcgtcggt gcggtcaccc
      781 gagaagcccg cgccgcagcc gatgagcagg ggttgttgcg tcatgccacg gactctatag
      841 tggaggcggt tatcggggaa atcgttattt ccgatggatt gatcgatgtc gcaactcaat
      901 atgtcgaccc ggcacctgcg cgccttcctg gccctggcgg aactcaggag cttcactcgc
      961 gccgcgaacc gctgccacct gtcgcagccg gcgttcagcg cgctggtgcg ttccctggag
     1021 gattcgctgg gcgcccgtct cttcgatcgc gatacgcgga acgtggaact caccgccgag
     1081 gggcgcctgc tcgaggagcc ggcccggcgc ctggtggccg acttcgaggg gctggtgacc
     1141 gacatcggcg accgcgtggc gcgccgcaag gggcgcgttt cggtggctgc gctgccatcg
     1201 ctcgcggcag ggtggctgcc gggcatcttc gccgaattcc gccgagccaa tcccggcgtc
     1261 gatctcgccc tgcacgacac gctttccgac cagtgcctgg acctcgtgcg cgccgggcgg
     1321 gcggacttcg ccatcgccgc caccgggccg gagagccccg agtggtcgac ggaggtgctc
     1381 tgccgcgacc gcttccacct cgtttgccgg cgcgaccatc cgctggcccg gaaggcgttg
     1441 ctgcacgtga aggacctggc cgcctacccg ttcgtccatt tctcgcgcgc gagcagcgtt
     1501 cgccagcacc tcgaggccgc gctgcatccc cagcaaatga gcacggtgct cgaggtggag
     1561 cacctcacga cggtgatggg catggtcgag gagggcgtgg gcatcaccgt cgtgccggcg
     1621 ctcacgctct tcgaattcga gcgcggctcg ctcatgcacc ggcccctcgc ggttgcgaac
     1681 ctgaagcgca ccatttacat agttcgccgg gcggaccgga gcctgtcgcc tgcagcccag
     1741 gcgctctacg acttgatgat cgagcgcaag cccgcgccgc gccggacttg aaccagttca
     1801 agaataatcg ccttcgccgt tcaatgcctg aaccgccggg cagtatcggg agtctctacc
     1861 gggccactct catacagaag gagattccaa tgaagatcat cgcgacgctc gtcgccaccg
     1921 gcgctttcgt catgtccgcc gccctgctgt cgcccctggc cgtcgccgtc gatacacacg
     1981 accacggtca cgccgcaggc gccgcgaagc ttcagctcga ccatggcaag aagtgggcga
     2041 cggacgcgcc actgcgccag ggcatgaccg caatccgcga ggcggtgcac ggcgccccgg
     2101 ccccgctgca caaggccacc gcgaagcccg aggcctacgc ggaggtaggc aatcgcgtcg
     2161 aggccgaagt cgggcgcatc gtgaaggaat gcaagcttcc gcccgaggca gacgcgcaac
     2221 tgcacctggt gattgccgac gtgatcgcgg gcgccgacgc gatgaagggc gccagggacg
     2281 cgaaggccgg gcgcgcaggg ctcgtcaagg tggacagcgc gctcaagaat tacggcacgt
     2341 acttcgacca cccgggttgg tagggacagg tatcggggac ggttctccgg aaccgtcccc
     2401 atttcttttc ccctacaatc gcgtctccat gtccgaccgg cacgacgcgc ccaccatccc
     2461 gcccggtttc cgcgccattg cgatcggcgg gccgttcatg cagaccgtcg ggcccctcta
     2521 tggacgctgg gacggcgagc gcgttcgcct gggcttgcgc gtcgaggagc gccacgcgaa
     2581 catcgccaag gcctgccacg gcggcatgct cacctcgttt gccgacatgc agatggcggt
     2641 ggcgacccac taccagtggc cggagatcgc gggccacagc ttcccgacca tcaatctcac
     2701 caccgacttc gtcgctcccg taccgctggg cgaatggctc gagggctcgg ccgacgtgat
     2761 ccgcgccacg aagagcctcg tcttcatcca gggcaccgcc gcggtggatg gcgccacggt
     2821 gctgcgcttc agcggcgtgt acaagatcgg gccgaagcgc gattacgcga acccgcgcga
     2881 ccccttcggc ctcctgccct tatcatcttc cggaaggtaa tgaccatgga ccagcccgtc
     2941 gtcatcgcgg cagcgatcac cggctccgtg ccgcgcaaga aggacacgcc cgccataccc
     3001 gtcatcccgt cggagcagat cgagagcacg caccaggcct tcgaggccgg ggcgagcctc
     3061 gtgcacattc acgtgcgcaa tgcggacgag tcctcggggt cggatcccgc gaaattcgcc
     3121 gaggtgcagg aaggcgtgaa gaagcattgc ccggggatga tcatccagtt ctccaccggc
     3181 gggcgcggcc gggaggcgag ccagcgcggc gcgatgctgc acctcaagcc ggacatggcg
     3241 tcgctcgcca cgggctcggt gaacttcccc acgatgatct acgaaaaccc gcccgatttc
     3301 atcgacaagc tcgccggccg gatgctcgag ctcgacatca agccggagtg cgagatcttc
     3361 gacctcgcca tgctctacaa cgcggcggac ctggtgaagc gccagctcct gaacgccccg
     3421 ccccacgtgc agttcgtctt cggcatcaag ggcgccatgc ccgcgcgccg ttcgatcctc
     3481 gagttcctcg tctccgagct caacatcgtg ctgcccggcg ccacgtggac ggcggccggc
     3541 atcgcgcggc atcagttcga ggtggcgcaa tgggcgctcg agatgggcgg gcactgccgc
     3601 acgggcctcg aggacaacat ccgctacgac gagacgcggc ttgccaggag caacgcggag
     3661 ctcgtggaaa aactcgttgc cgaatgcgcg cggcgcggcc gtcgcgtggc cacgccggcg
     3721 gaagcaaggc agatcctggg gctgcgcccg gcctgattgc cggcttcccg gtcctatcct
     3781 tcgaccacca ccgtacccat catcccgccg gccgcgtggt cgaggatgtg gcagtgcaac
     3841 atccactcgc cgacgttttc cgcgacgaac gcgatgtcgc aactcccacg cgggtcgatc
     3901 atcacggtgt cgcgccattc gcgcagcttc gtcggcacgt cgttcagcgc gagcacgcgg
     3961 aagaaatggc cgtgcaggtg catcgggtgc tcgtagtcgg tgttgttgat catcttcagc
     4021 gtcacgtgct cgtccttgcg catgcggaag agcggctcgt gcatcatggc gagctcgtgc
     4081 tgggcggtgt agttcatcgt ccaggcgagc ccgtgcttct ccatgatttc ctcgaccttg
     4141 agcgccttgc cgtccacctt gccgatggcg ggcgcaccct tcatgccgcc ctcgaagacg
     4201 aggtagtggt ccgtggcctt gccgacgtcg ggctcggtgt ggcggttggg aacgatggct
     4261 gcgggccggc cgaggacctt cgcgcgcacg ggcttgccgt cgtaggcgat gcgcgcgatc
     4321 tcgaagccgc cgcggcgatg ttccgtcacg gcgaacgact gcccggcgga gccgacgcca
     4381 tcgatcacca ggtccgttcg ttgccccgcg ccgagcagca gccgcccgtc cggcaccggc
     4441 tgcggcgcga tcgcctggcc gtcgtagctc acgatccagg gcgaatggcc ggtgaactcg
     4501 aggaggaagg cgcgcgcgga agctgcgttg agcaggcgaa ggcgcacgcg ttcgccaggg
     4561 cgaagctcca ggcgccggcc tggcccggcg gccgtgccgt tcagcatgac gacgttgccg
     4621 aaccggcctt cgctgccgaa atcgagcatc ttgccgaagt cctccaccgc caggttgtcg
     4681 tcgccgagct tgtagtcgga cagcacccag cgctcctcgc ggtccacggg aaggggcttg
     4741 cgctcctcga cgacgagcat cccgtagagc ccgcgcggca cctgctcgaa gctcgactgg
     4801 tgcgggtggt accagaacgt gccggcatcc tcgacacggt agcggtagtc gaaggtgccg
     4861 cccttcgcga tcggaacctg ggtcacgaac gggacgccat ccatggcgtt gggaacgcgc
     4921 acgccgtgcc aatggaccgt ggtcgcctgc ggtagcgtgt tcgtcacctg gatatcgacg
     4981 gggtcaccct gccggaagcg aagcagcggc ccgggcacgg agccgttgaa gccccacacc
     5041 tccgtctcct ggcccgaggg ccggatcttt tgccggaaca cgcccgcggt gagttcgagg
     5101 cggggcgggg cggccagcag ggtggtcgac gggatcgcca gcgcgcccag ggcggcaccg
     5161 gccgattgca ggaattcgcg acgcgtgtgg ctcatcggtg ttctcctcct ggcgcggccg
     5221 catcgaggcg gcgcgcgaga aaacgcttct caccggtgag acgaaaatgg gtcgctcacc
     5281 tccgggccga ccctcggaac gggttcggct ggaacgtcga agcaaccttc acaccagttc
     5341 cgccggcgtc ccgtcgcgga catcccggcc tggcaacgga tcagaaggcg accctgatga
     5401 aaagcccgac cgtgctcgca ctgtagttgc ccggcccgcc gtcgaggtaa gccgcgaagg
     5461 catccggggc gggattgccg gccacgccgt cgtagtgcca gtcgctgatc cggccgcgct
     5521 cgtagcggta gtacaggcgt gcggacatcg acctgccgat cgggtaggtc aggttcgcct
     5581 cgaccgtgtt ctgtaccagt cgcgcctcgg gaaagccgga gccgacgagg gccacctccg
     5641 cggcggagat cgatagcgcg gctgcattgt agagataggt cgacgtcgag cggccgctcg
     5701 cgtaggtgta gctcgcatcg agcttcgcca gcccgaaatc gcgtcgcaac ccgatgctcg
     5761 ccgcgtgatt gcgctgctcc tggaactgct cccacgagtt gccggccggg aacagcggac
     5821 tcaacggccc cgcggctgca caggtcgcga tcgcgttggc agccgtgacc tgcgtcgaac
     5881 caatgcgtgt tgcaccggcc ggcgcaatgc ccgtcgtgtt gacctcgccg ttgctgtaga
     5941 agaagtacgt gaccccggcc acgcacgagt tggcctgcag gcccgtctgg tgcatcttcc
     6001 gggattgcca cgtgtagtag ccgtagaggg cgaattcggc ggaggcctgc cagttggctt
     6061 ccacgctgac cgaagcgtcc cggttcgtgc cgtttcgccc gaactcggca tccgggtagc
     6121 gcgtatcccg gaactgcgcc gacaacccca cgtccacgtc gggcatcacc gcccagttga
     6181 tgcgcccgtt cgccgtgcgc gaatcccggt cggcgaggtc gaaacggcga aagctgtcaa
     6241 tggcgtggat ccacgtcgcg aagttccccg tggcgcccgt cggcagcggg cccatcgagg
     6301 cgctcaggaa ttcctcgtaa gggttggcca cataggcgct gccacggcgc cgcgcattct
     6361 ccagggaaaa gagcagggtc acgttgtcga agccgcggtt ggtgaatccc actttcacct
     6421 tgtcttcgcg ggtcttgtcg cgctcccggt gctcccggtc gtactcctcg cgctcgaagg
     6481 atcctgtcac gttggacctg gccgtggccc gccaatccgc cgccagcgtg tagttggtct
     6541 gcgtgtactg gaagggaacg gcgcggatgt tgatgttgcc ggcgctgggc gacacgccaa
     6601 gggcgcgcac cgccgcgagg tcgcaaccgg ccgcgaggta cgcaggggtg ttcacgaagg
     6661 cgccgccgct gccgtcgttg aggagccggc cccattggcc ggtgagcggg ttgcaggcga
     6721 ggaagtcggt ggagttgtcg gtttcgtagc ggcgcaggtt cgcgcgcagg gtcgcgctcg
     6781 gcgcggggtt gagcgaaagg ccgaggttca ccagcgtggt ctcgatctcg gcgcccgatg
     6841 tgcggcggct cagggaatcg agcgtgttcc agcgattcgc ggcggaaatg ccgttgatcg
     6901 tgccgccggt aagcggcagc gccgtcggtg caatgagcgc atcgtcctgc tgcgatcgcg
     6961 tggccgccac gacggcactg aagcgcccgt tcatgagcga cggcaggttg cgcgcgtatt
     7021 ccgccttcgc cttgtagtgg ttgttgtcgg gatacatgtc gaatcgcgcc gacatgaatg
     7081 tgccggccgg cacgccggct accgtgttca cggcgatggc gagcgggttc tcgacggtga
     7141 gcgtgtcgat ggcgttgcgg tagagcgagc cctgcagcgt caggttgaaa ctgttcacgc
     7201 ggtcgaaata gctgatcccg ccctggaaat cgtgggtggt ggaatcgacg gactcggcga
     7261 actcgaagtt gccgccgccg tcgccggcgc cgaacgtcgc ggcgtagggc gcggcgcccc
     7321 gcttcttctc ggccgtgtag tccgcataaa gcttccaggt ttcggagagc gtcacgtcga
     7381 cacgcacgcc gcctttcttg cgcgtgatcg cgagttccgt ttccggcgcc gcggcgatcg
     7441 catttcgcag cgccgccagc gaggcgtccg gcgtggcctg accgcccgga gtgagcgtgg
     7501 cgagcgtctg ccggtccgtg ccgatgccgt tccacagcga ccggaaggtg ttggtcgcga
     7561 cggacggcgt ttcgttgaag aacgccctca ccttccacgc gttgtggcgc ccgaaggcga
     7621 tcgagtggaa ctggtcgtcg cgtgccacgg acccggcgtt cgcgcgcacg aaggtcgcgg
     7681 tatcgggctt ttcggcgtcg accgcaaggt ggtggaggac gaggccggag tccacgtccc
     7741 gatagcgccc gaacagcgga ttgcggtggt cgccgcttgc gtcgaggccg ccgaactcga
     7801 cctcggcccg gtacagccag ccgtcctgca ccgtctttcg cggctcggac aggaggtcac
     7861 gcggcccgaa gaggatgccg atggggctgc gcttcgggtc gatgtcggcc tctacccgcg
     7921 gcgggcctgc ggcaatgccc tgggacatcg cgttgccggc ggacgtgtcc gtgccggaga
     7981 cggaatcggc gcgcgtcgcg acgggggcga aggcgacgcc caggccagcg accaggaccg
     8041 tggcgttggg gcgaatgagc gtcgcgagtc gcccgatgca ttgcgcggct gtcgtcctca
     8101 tgtgcggttc tccttcgcgc tagcgctgca ggcgtgcgcc ggacggatga ttgctgccat
     8161 ggatggccga gtggcagttc tggcaggagc gcccgatgac gcgcgggctg ggcgtgccgc
     8221 cggccagcgc ggcagcggta gtctggtcgc cgcgatagaa cgcgctcgaa tggcccaccg
     8281 gaaggttgtg gcactgctgg cagaggaacg ggcgcgcgga gacgaggagc cgctcgtggt
     8341 tggagccgtg cgggtcgtgg cagttggcgc agttctcgcg aaccggcgca tgttcccaaa
     8401 ggaaaggccc gcgcttctcc gcatggcagg caacgcaggt ctcgtttacg ctgtcctgcc
     8461 ttagcaacgg ccgcgtgatc gagccgtgcg gattgtggca gtcgacgcag ctcatcttgc
     8521 cctcgagcac cggcatgtgc gagcgcttct ggaactgcgc gcgctgttgc gcatggcagg
     8581 caaggcacgt ttcgttgatg gtgggccggc gcagcaggcc gtccgtcgag cgccgctcca
     8641 tcggcgcgtg gcagtcgctg cacgcgacga ggttctgcgc gtgggccgag cccggccaat
     8701 gctggcgctg cccgccgccg tggcactgca ggcactgcgc gttcatcgtt tcgacggggg
     8761 tggcggactg gcgcgtgaag cggatgggag cggacttgtc ggcggcgttg gccacgtgct
     8821 tcgagccggg gccgtggcag gcctcgcaga cgcgcctttc gaggtcgttc ttcggattgc
     8881 cgcggaaggc ccgggcgtgc ggcgtctcgg tgaaatggcg gttgttggcc tcgtggcagg
     8941 tgatgcacct ggcctcgccg acgtactcgg cctcttgcgc ccgggccacc acgccctgca
     9001 ggcacgaggc ggcaagcagg agcagcccgg cgcatccggt ccatcgcctc atcgcacgac
     9061 ctcgctcctc ccgtgggccg cgcaaatcgg cccgatcggg tgggaacagc aggagtggcg
     9121 gcgatattcc ccgttcggca tgacacaatt cgcgcattcg ccggcgattt cgcgccactt
     9181 gcaacggccg cacacgattg gcgtcctgcg ggacggggag gaacccatga aatcgctgaa
     9241 tcgccttcgc gccattgcgc tcttgtccgc ggcgctcgcg gcgcccgcgt ggagccaggc
     9301 gccatccgtg acgccttact atcccgatgc gcagtggcaa cgcaagtcgc ccgcggaggc
     9361 ggggatcgat gccacgaagc tgaaggatgc gatcgatctc gccattgcat ccgagtcgcg
     9421 caatccgcgc gacctcacgc tgaaccacta tcggacgttc ggccgcgagc ccttcggcat
     9481 cgccatcggg ccgatcaggg atcgcggcga catgacgggg gtcatcgtgc accgcgggta
     9541 catcgttgcg gaatggggcg agccctcccg ggtggacatg acacacagcg tgacgaagag
     9601 cctgctttcg agcgtggtcg gcgtggcctt cgaccgcggc atgatccgca gcctcgagga
     9661 cccggtccgt gactacgtgg cgccgaccct cgtgtaccgt ccgccggctg cggccagcga
     9721 caaggccgag cagctcgggc gctccgatct cctttggcta ttcgaaacgc cccacaaccg
     9781 caccatcacc tgggaccatc tgctgcgaca gaccagtgac tgggaaggca ctttgtgggg
     9841 gaagccggac tgggccgacc gtcccggcga cacgcccgcc gaatggacca cgcgcgcacg
     9901 caacacaccg ggcactgcgt acaagtacaa cgacgtccgc gtgaacgtgc tcgcccttgc
     9961 cgccctcaac gtctggcgcc gcccgctgcc gcaggttgcg aaggaacaca tcatggaccc
    10021 gataggcgcc tcgaatacgt ggcgctggtt cggttacgag aactcgttcg tcgtccttga
    10081 cggggcgatc gtgcagtccg tgaccggtgg tggccattgg ggcggcggcc tgttcatcaa
    10141 tgcgtacgac atggcgcgct tcggttatct cacgttgcgc aagggccgct ggaaggaccg
    10201 ccaggtcctc tccacgcagt gggtcgatcg ggcgctcacg ccaacgcctg tgcagccggg
    10261 ctacggcttc atgaactggt acaacaaccg cgaacggaag ctgcttccga gcgcccctgc
    10321 cagcgccttc gcccacatcg gcaacggcac gaacatcatc tatgtggacc ccgagcacga
    10381 tctggtcgcg gtgctgcgct ggatccagag tcccgcgatc gacgcagtcg tgcagcggct
    10441 catcgaggcg ctgccggcac gctagccggg tgttccgcct tattcgaccg taacgctctt
    10501 ggcgaggttg cgcggcttgt ccacatccgt gccgcgcctg agcgccgtgt ggtacgcgag
    10561 gagctggacc gggatcgtgt gcacgatcgg cgagaggagc cccacgtggc gcggcgtgcg
    10621 gatcacgtgc acgcccgcgc tttccgagaa gtggctgtcg aggtcggcga acacgtagag
    10681 ctcgccgccg cgcgcgcgca cttcctgcat gttgctcttc accttctcca gcagcgagtc
    10741 gttgggcgcg atcaccacca ccggcatctc ggaatcgacc agggccagcg gcccgtgctt
    10801 cagctcgccc gccgcatagg cctcggcgtg gatgtaggag atttccttga gcttgagcgc
    10861 gccttcgagc gccaccggat agtgaatccc gcggcccagg aacagcgcgt gctccttgtg
    10921 cgcgaagcgc tcggcccaga cctgcacctg cggctcgagg ttgagcgcgt gctggacgct
    10981 gccgggcacg aagcgaagct gcgcgatggc ggcggcctcc gagttcggat cgaggcggcc
    11041 cttcgccttg gccagcgcca gggtgagcgt gaagagcgcg acgagctgcg tggtgaaggc
    11101 cttggtcgaa gccaccccga tttccatgcc ggcgcgcgtg tagaacacga atcgcgaggc
    11161 gcgcgggatg gcgctttcgt gcacgttgca gatggagagc gtgttcgcct gccccagctc
    11221 cctggcgcgc ttcagggcct ccatcgtgtc cagcgtctcg cccgactgcg agatcgtcac
    11281 cacgagctgc ctcgggttgg gaatcgagtc gcggtagcgg tattcgtggc ccagctccac
    11341 gttgcagggc accttcgcga tcgactccag ccagaagcgc gcgacgaggc cggcgtagaa
    11401 gctggtgccg gaggcgagga tgagcaacgc atcggtgtcg cgaaaggctg cggcggcgcc
    11461 cgtgccgaag agcccgtcca catcgacgcc gccgtctatc accgcttcca gcgtgtccgc
    11521 cacggcgcgg ggctgctcgt ggatctcctt ctgcatgtag tggctgtagg gcccgaggtc
    11581 cacggcgtcg gccgacagcg tgctcaggcg ctccggccgg ttgacgggct tgcccgcgat
    11641 gtccacgacc gagacaccgc tgcggtggac tgcaaccacg tcgccgtcct cgaggtacac
    11701 cacgcgccgc gtttccgaga cgatggcgga gacgtcggag gcgaggaagt tctcgctttc
    11761 gccgaggccc accaccaggg ggcagcccat gcgcgcgccg gtgatcgttt ccgggtcctt
    11821 gagcgccagc acggcaatgg cgtaggcgcc gcgaaggtcg gcggcagcct tctgcgtggc
    11881 ggcgaagagg tcgcccgtcg ccttgaagta gtggtgcacg aggtgggcga tgacctcggt
    11941 atcggtctgc gattcgaagg cgtagcccag gaccttcagg cgcgcgcgct gctcctcgtg
    12001 gttctcgatg atgccgttgt gcacgacggc gatctcgttg gaggagatat ggggatgcgc
    12061 attgggctcg gtgacgccgc cgtgcgtggc ccagcgggtg tgcgcgattc ccacgatgcc
    12121 ggaaaggttc tccgccttgg cggcgccttc catttccgtc acgcgcccga cgcggcggac
    12181 tcgccggatg ccgccgtcca ggatcgcgat gcccgcggaa tcgtagccgc ggtattcgag
    12241 tctcttcagc ccctccgtca ggatggggac gacgttgcga ttggcgatgg cgccgacgat
    12301 gccgcacatg ggcgtgattt tctcacgaaa gaaacgaata cccgaaaaaa ctcccgctca
    12361 ggtcacacca cgagccggat gcgacatgcg gccaaggatt cggcttgcga cgacggccgc
    12421 aacaagtgta gccaggaccg cggccggggg cgcgtaccag cccaggcgca gcgcgaaaag
    12481 ggcaccggcg atacaaccgc ccgccgccag cgcgccgacc gccggcgcca cgggggccgc
    12541 aatcagcgcg acgagggcag cggctgcggt tgccaggagc attgccggca gcggcgcctc
    12601 gtggatcggg ttcccatgca acagggtacg caccgtctgc gcatgcgcca gcaccgcggc
    12661 cggctcccgg gatggtggtt cccagccggc gagggacacg ggttgcggaa cgcggtcgtc
    12721 gggccccgtg accggcgcca cgaacacgac gcgaccgcgg aacagcctcg ccagggcagt
    12781 gtcgtcgcga atgtcgagga gccgctgcgc gggcaggtag cgataggcgg gcccgagcgc
    12841 gtagtcgatg agtcccggct cgcacttgcc gccgagcacc gcgcaaagcc agccggcgga
    12901 tgtcggccac ttcccctgct gggtaggcag ggcgagcacg atttgccgca cgacgccgtc
    12961 ctcgtcgcgc ggcaagagcg tgaacacaag cgcttgcgta tcgactccgc gcaacaaggc
    13021 atcgtggata ggcactacct cgcgccgtga atcgacgctc atgcctaccg cgagcggtgc
    13081 agcggcctgg gccacggaaa gcgccgtggc aagcgcgtca tcccatcccg gtgccgctgc
    13141 ctcctgcgag gctcgtggca gccgcgcatg cagggcgatg gctcgcggtc ggccgcgcgc
    13201 gatacgcacc agcacgtccg ggagctggcg cagcccggga cccgccgaac ccacctccgc
    13261 cacggtggcg gcatcgtgga gccccacgat gaggatatcg tcgggcgatt tcccgggcgc
    13321 gatcgcgcgc aggatgccga agcccgcgtc gagcatcacg aggctcgctt tcgcacccca
    13381 gggggacaga tggaaagcga ggcccgca
//
LOCUS       DASUEV010000252         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_171635, whole genome shotgun sequence.
ACCESSION   DASUEV010000252 DASUEV010000000
VERSION     DASUEV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171635"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2632)
                     /gene="acnA"
                     /locus_tag="VFV55_08115"
     CDS             complement(<1..2632)
                     /gene="acnA"
                     /locus_tag="VFV55_08115"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX4944307.1"
                     /translation="MPHNAFDTLKTFQPAPGKSGRFYSLPALAETFPNVKRLPVSIRI
                     VLESVLRNVDGKKVAEEHVKQLANWGATASRSNEIPFVLARIVLQDFTGVPLLADLAA
                     MRGVAAKMGRNPKVIEPLVPVDLVVDHSVQIDHYGSPGALDLNMKLEFQRNRERYEFM
                     KWGMQAFDTFKVVPPGIGIVHQVNLEYLARGVHQKGGVYYPDTLVGTDSHTTMINGIG
                     VVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNITGRLREGVTATDFALTVTEMLRKAKV
                     VGKFVEFFGEGAETLAVTDRATIANMAPEYGATMGFFPVDARTIEFFRNTGRTADEVS
                     ALEAYFRAQELFGMPKKGDIDYSTVLELDLASIRPSVAGPKRPQDRIDLGDLKAKFTQ
                     LYSTPVAENGFSKKAGDLATRVRTTDGIDLGSGDVLIAAITSCTNTSNPSVLIAAGLL
                     AKKAVEKGLTVKPHIKTSLAPGSRVVTEYLRAAGLLGSLEKLGFDVAAYGCTTCIGNA
                     GPLAETIEKAVVDNDLVCAAVLSGNRNFEARIHANIRANFLASPPLVVAYAIAGTVLR
                     NLETEPLGQGKDGPVYLKDVWPTSDEIQALMKFATDPAVFTRMYANLADTNPLWRGIA
                     SATGQVYNWPKSTYIAEPPFFAGFGMAAGTAKDVRGAHVLGIFGDSVTTDHISPAGSI
                     KDTSPAGKYLIGNGVMKPDFNSYGARRGNHEVMMRGTFANVRVKNLMLPLRADGSRVE
                     GGFTLLQPSGEQMPIYDAAMKYVAGGTPTVVFGGEEYGTGSSRDWAAKGTQLLGVKAV
                     VARSFERIHRSNLVGMGVLPCQFKGSDSAESLGIRGDETFDIEVPSEIKPQQDLVLGV
                     TGKDGKRREVEVLCRIDTPIE"
     gene            complement(2675..3142)
                     /locus_tag="VFV55_08120"
     CDS             complement(2675..3142)
                     /locus_tag="VFV55_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944308.1"
                     /translation="MTRKPAITAAARMLLAGAIAAAAGGCATNYQLTLMPRDSGKVYV
                     GVAEDSGGGEGTMSVTIEGRTYAGTWVEVIPSRSMGYVSGGYGGRHRGFGLGGVISMD
                     NPYGGAAKALLRAADGAGLRCDLRGGSGQPGGGVCRDDKGLEYDVQIRVAPRQ"
     gene            complement(3205..>3457)
                     /gene="prpD"
                     /locus_tag="VFV55_08125"
                     /gene_synonym="mmgE"
     CDS             complement(3205..>3457)
                     /gene="prpD"
                     /locus_tag="VFV55_08125"
                     /gene_synonym="mmgE"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168018.1"
                     /note="functions in propionate metabolism; involved in
                     isomerization of (2S,3S)-methylcitrate to
                     (2R,3S)-methylisocitrate; also encodes minor aconitase or
                     dehydratase activity; aconitase C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HEX4944309.1"
                     /translation="RSIANALTVEFRDGKKMKEIVVEYPIGHKRRRKEGMPVLVEKFK
                     VNLARRFPSKQQKAILELCLDQKKLEATPVNEFVDLMAV"
BASE COUNT          487 a         1139 c         1214 g          617 t
ORIGIN      
        1 cctcgatggg ggtgtcgatg cggcagagca cctccacctc gcgccgcttg ccgtccttgc
       61 ccgtgactcc cagcacgagg tcctgctgcg gcttgatctc cgagggcacc tcgatgtcga
      121 aggtctcgtc gccccggatg cccagcgact cggcggaatc cgaccccttg aactggcagg
      181 gcagcacgcc catgccgacg aggttcgagc ggtggatgcg ctcgaagctg cgcgccacga
      241 ccgccttcac gccgaggagc tgcgtgccct tggcggccca gtcccgcgac gacccggtgc
      301 cgtactcctc gcctccgaag accaccgtgg gcgtgccgcc cgcgacgtac ttcatcgcgg
      361 cgtcgtagat cggcatctgc tcgccggaag gctggaggag cgtgaagccg ccttccacgc
      421 gcgagccgtc ggcgcgcaag ggcagcatga ggttcttcac gcgcacgttg gcgaaagtgc
      481 cgcgcatcat cacctcgtgg ttgccgcggc gcgcgccgta gctgttgaag tccggcttca
      541 tcacgccgtt gccgatgagg tacttgccgg ccggcgaggt gtccttgatg gaacccgcgg
      601 gggagatgtg gtcggtggtg acggagtcgc cgaagatgcc gagcacgtgc gcgccccgga
      661 cgtccttcgc cgtgcccgcg gccatcccga atcccgcgaa gaacggcggc tcggcgatgt
      721 aggtcgactt cggccagttg tacacctgcc cggtggcgct cgcgatgccc cgccacaggg
      781 ggttcgtgtc ggcgaggttg gcgtacatcc tggtgaagac ggcggggtcg gtcgcgaact
      841 tcatcaacgc ctggatttcg tcggaagtcg gccagacgtc cttcaggtag accgggccgt
      901 ccttgccctg cccgagcggc tcggtctcga ggttcctcaa caccgtgccg gcgatggcgt
      961 aggccacgac caggggaggc gaggcgagga agttcgcgcg gatgttggcg tggatgcgcg
     1021 cctcgaagtt gcggttgccg gaaaggaccg cggcgcacac caggtcgttg tccaccaccg
     1081 ccttctcgat cgtctcggcg agcgggcccg cgttgccgat gcacgtcgtg cagccatagg
     1141 cggcgacgtc gaagcccagc ttttccagcg accccagcag tcccgccgcc ctgaggtatt
     1201 cggtgaccac gcgcgagccg ggcgcgagcg aggtcttgat gtgcggcttc acggtgaggc
     1261 ccttctcgac cgcctttttc gccagcaggc cggcggcgat gagcacgctc gggttcgagg
     1321 tgttggtgca cgaggtgatg gcggcgatga gcacgtcgcc cgagccgagg tcgatgccgt
     1381 cggtggtccg cacgcgcgtg gcgaggtcgc cggccttctt cgagaagccg ttttcggcca
     1441 ccggcgtcga gtagagctgc gtgaacttcg ccttcaggtc gccgaggtcg atgcggtcct
     1501 gcgggcgctt cggccccgcc accgagggcc tgatcgacgc gaggtcgagt tcgagcacgg
     1561 tggagtagtc gatgtcgccc ttcttgggca tgccgaagag ttcctgcgcc cggaagtagg
     1621 cctcgagggc ggaaacctcg tcagccgtgc gcccggtgtt gcggaagaac tcgatggtgc
     1681 gcgcgtcgac ggggaagaag cccatggtcg cgccgtattc cggcgccatg ttggcgatcg
     1741 tggcgcggtc ggtgaccgcc agcgtttcgg cgccctcgcc gaagaactcg acgaacttgc
     1801 ccacgacctt ggccttgcgc agcatctccg tcacggtgag cgcgaagtcc gtggcggtga
     1861 cgccttcgcg cagccggccg gtgatgttca cgcccaccac gtcgggcgtg aggaagtaga
     1921 ccggctggcc gagcatgccg gcctccgcct cgatgccgcc cacgccccag cccacgacgc
     1981 cgatgccgtt gatcatcgtg gtgtggctgt cggtgcccac gagagtgtcg gggtagtaca
     2041 cgccgccctt ctggtgcacg ccgcgcgcga ggtactcgag gttcacctgg tgcacgatgc
     2101 cgatgcccgg gggaacgacc ttgaacgtgt cgaaggcctg catgccccac ttcatgaatt
     2161 cgtagcgctc gcggttgcgc tggaactcga gcttcatgtt gaggtcgagc gcgcccgggc
     2221 tgccatagtg gtcgatctgc accgagtggt cgaccaccag gtccacgggc acgagcggct
     2281 cgatcacctt cgggttcctg cccatcttcg cggcgacgcc gcgcatggcg gccaggtcgg
     2341 cgagcagtgg cacgccggtg aagtcctgca gcacgatgcg cgccagcacg aacgggatct
     2401 cgttgctgcg cgaggccgtg gcgccccagt tggcgagttg cttcacgtgt tcctcggcga
     2461 ccttcttgcc gtcgacgttg cgcaggaccg attcgagaac gatgcggatc gagacgggca
     2521 ggcgcttcac gttcgggaac gtttccgcca gcgcgggcag ggaatagaac cggccggact
     2581 tgccgggggc gggctggaag gtcttgaggg tgtcgaaggc gttatggggc atggggtcgt
     2641 ctcgggttgt cgttcggtat gcggtcggtg tttgctattg gcgcggggcg acgcggatct
     2701 gcacgtcgta ctcgaggccc ttgtcgtcgc ggcagacgcc gcctcccggc tggccgctgc
     2761 cgccgcgaag gtcgcagcgc agcccggcgc catcggccgc gcgcaacagc gccttggccg
     2821 cgccgccgta gggattgtcc atcgaaatca cgccgccgag gccgaagccg cggtgccttc
     2881 cgccgtagcc cccggacacg tagcccatgg agcggctggg gatgacttcc acccaggtgc
     2941 cggcgtaggt gcggccctcg atggtgacgg acatggttcc ctcgccgccg ccggaatcct
     3001 cggccacgcc cacgtacacc ttgccgctgt cgcggggcat cagggtcagc tggtaattcg
     3061 tggcgcagcc gccggccgcg gccgcgatgg cgccggcaag cagcatccgg gcggccgctg
     3121 tgatcgcggg tttcctggtc atgtttcctc cttgcggccg gcggggactt gcggccctgt
     3181 tccggcgcgg tgtgtcgctc cccttcagac ggccatcagg tcgacgaact cgttgaccgg
     3241 cgtggcttcc agcttcttct ggtcgagaca gagctcgagg atcgccttct gctgcttgga
     3301 cgggaagcgc cgggcgaggt tcaccttgaa cttctccacg agcaccggca tgccctcctt
     3361 gcggcggcgc ttgtggccga tggggtactc gaccacgatc tccttcatct tcttgccgtc
     3421 gcggaactcc acggtgagcg cgttggcgat cgagcgc
//
LOCUS       DASUEV010000253         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1655947, whole genome shotgun sequence.
ACCESSION   DASUEV010000253 DASUEV010000000
VERSION     DASUEV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655947"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="hppD"
                     /locus_tag="VFV55_08130"
     CDS             <1..858
                     /gene="hppD"
                     /locus_tag="VFV55_08130"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX4944310.1"
                     /translation="HGPSACAFAIRVRNAAKAWQRALDLGAKPHHGPVGPMELNIPAI
                     KGIGGSLIYFVDRYPGNGGNLSIYDVDFVPVEGAGQQPAGAGLLEVDHLTHNVYAGRM
                     DVWAQFYEKLFDFREIRYFDIKGKKTGLTSRALTSPCGKIRIPINEPSDRKSQIQEYL
                     DAYHGEGIQHIALSTADIYATVDLLRERKVRFLDTPDSYYDRVDARVKDHGEDLARLR
                     RSRILIDGEGVDKERHEDKLLQIFTETMIGPIFFEIIQRKGNEGFGEGNFRALFESIE
                     AEQIARGVV"
     gene            988..2277
                     /gene="hmgA"
                     /locus_tag="VFV55_08135"
     CDS             988..2277
                     /gene="hmgA"
                     /locus_tag="VFV55_08135"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX4944311.1"
                     /translation="MAGDLHYMSGFGAHHESEAVAGALPVGQNSPQKTPFGLYAEQFS
                     ATSFTAPRARNLRAWLYRLRPSAMHPAFRRIDDGRLRTGPCPEAQAGPNRLRWAPMAM
                     PDGPTDFIDGLATLATNGEARLQSGVGVHLYSANQSMERVFASFDGELLLVPQEGTLA
                     IATEFGHLAVAPGEIAVIPRGVKFRVAVAGPVRGYACENYGAPLRLPDLGPIGANGLA
                     NPRDFLAPVAAFEDRDVPTEVVVKSGGHLWTAGYGHSPLDVVAWHGNVHPFKYDLARF
                     NAINTVSFDHPDPSIFTVLTSPSDTPGTANVDFVIFPPRWLVAEHTFRPPWFHRNVMS
                     EFMGLVHGEYDAKRGGFVPGGASLHNAMTAHGPDRASHEAAVAAKLDPKHLGHTLAFM
                     FESRHPFDPTAFAMASPALEKDYDAAWAGFPAVRLAK"
BASE COUNT          427 a          807 c          816 g          388 t
ORIGIN      
        1 cacgggcctt cggcgtgcgc cttcgccatc cgcgtcagga atgcggccaa ggcctggcag
       61 cgcgcgctcg atctgggcgc gaagccgcac cacggcccgg tgggacccat ggagctcaac
      121 atcccggcga taaagggcat cggcggcagc ctcatctact tcgtcgatcg ctatccgggc
      181 aacggcggca acctttccat ctacgacgtc gacttcgtgc cggtggaagg cgccgggcag
      241 cagccggccg gcgcggggct gctcgaagtc gatcacctca cgcacaacgt ctacgccggg
      301 cgcatggacg tgtgggcgca gttctacgag aaactcttcg acttccgcga gatccgctac
      361 ttcgacatca aggggaagaa gacggggctc acctcgcgcg cgctcaccag cccctgcggg
      421 aagatccgca tcccgatcaa cgagcccagc gaccgcaaga gccagatcca ggagtacctc
      481 gacgcctacc acggggaagg gatccagcac atcgcgctct cgacggcgga catctacgcg
      541 acggtggacc tgctgcgtga gcgaaaggtg cgattcctcg atacgccgga cagctactat
      601 gaccgcgtcg atgcgcgggt gaaggaccac ggcgaggacc tcgcgaggct gcggcggtcc
      661 cgcatcctca tcgacgggga gggcgtggac aaggaacgcc acgaggacaa gttgctgcag
      721 atcttcaccg agacgatgat cgggccgatc ttcttcgaaa tcatccagcg gaaggggaac
      781 gaggggttcg gggagggcaa tttcagggcg cttttcgagt cgatcgaggc ggagcagatt
      841 gcgcgaggcg tggtctagga gtgatgcggg ccaaaagcgt tggacacgga tgaacacgga
      901 tgaacacgga tgatcgtcat gagccgggcg aaaggcgatg gaaccgcaga tgaatgcaga
      961 tgatcgccat gtgaaggcgc ttgggtaatg gccggcgatc ttcattacat gtcggggttc
     1021 ggggcgcacc acgagagcga ggccgtggcg ggggcgctgc cggtgggaca gaactcgccg
     1081 cagaagacgc cgttcggcct ttatgcggag cagttttccg ccacgtcgtt taccgcgccg
     1141 cgcgcgcgca acctgcgcgc ctggctgtac cggttacggc cgagcgcgat gcatccggcg
     1201 tttcggcgca tcgacgacgg gcgtttgcgt acgggaccgt gcccggaagc gcaggcgggg
     1261 ccgaaccggc tgcgctgggc gcccatggcg atgccggatg ggccgacgga cttcatcgac
     1321 gggctggcca cgctcgcgac caatggcgaa gcgcgactgc agtccggcgt cggggtgcat
     1381 ctctattcgg ccaaccagtc gatggagcgc gtgttcgcga gcttcgacgg ggaactgctc
     1441 ctcgtgcccc aggagggcac gcttgcgatt gccaccgagt tcggccatct ggcggtggcg
     1501 ccgggcgaaa tcgccgtcat tccgcgcggc gtgaaattcc gcgtggcggt ggcggggccc
     1561 gttcgcggct acgcgtgcga aaactacggc gcgccgctgc ggcttccgga tctcgggccc
     1621 atcggcgcca acggtctcgc caatccgcgc gatttcctgg cccccgtggc ggccttcgag
     1681 gatcgcgatg tgccgaccga ggtcgtcgtg aagtccggcg gtcacctctg gacggccgga
     1741 tacggccatt cgccgctcga cgtcgtcgcc tggcacggca acgtgcatcc gttcaagtac
     1801 gacctcgcgc gcttcaacgc gatcaatacc gtgagcttcg accatccgga cccctcgatc
     1861 ttcaccgtgc tcacctctcc ctcagacacg cccggaaccg ccaacgtgga cttcgtcatc
     1921 ttcccgccgc gctggctggt ggcggagcac accttccggc cgccgtggtt ccaccgcaac
     1981 gtgatgagcg aattcatggg gctggtgcat ggcgagtacg acgcgaagcg cggcggcttc
     2041 gtcccgggcg gcgcgagcct gcacaacgcc atgaccgcgc atggccccga ccgcgcctcg
     2101 cacgaggcgg ccgtcgcggc gaagctcgac ccgaagcacc tcggccacac gctcgcgttc
     2161 atgttcgaga gccgccatcc cttcgatccc accgccttcg ccatggcctc gcccgcgctc
     2221 gagaaggatt acgacgcggc atgggccggc tttcccgcgg tgagactcgc gaaatgaaac
     2281 tggcatcgct gaagggcccc acccgcgacg gcacgctcgt gctcgtcaat cgcgaccttt
     2341 ccaccgcggt cgccgcgtcc ggcatctcgc ccacgatgca gcatgcgctg gagaactggg
     2401 aagcggtcta tcgcacgctc ctcgacgcgt acgagagc
//
LOCUS       DASUEV010000254         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1114803, whole genome shotgun sequence.
ACCESSION   DASUEV010000254 DASUEV010000000
VERSION     DASUEV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114803"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..64)
                     /locus_tag="VFV55_08140"
     rRNA            complement(<1..64)
                     /locus_tag="VFV55_08140"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(257..333)
                     /locus_tag="VFV55_08145"
     tRNA            complement(257..333)
                     /locus_tag="VFV55_08145"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(297..299),aa:Met,seq:cat)
     gene            482..1105
                     /locus_tag="VFV55_08150"
     CDS             482..1105
                     /locus_tag="VFV55_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX4944312.1"
                     /translation="MSPNDWQLLQAVALGSLLAWASGLRLYLVVFVLGLAARLGYLDL
                     PDGLAILSHTYVIAAAGLLLAIEFLADKVPGIDSAWDAVHTFIRIPAGALLAAGAAGD
                     DLTAITVAAGLLGGTITAGTHFAKAGGRVAINASPEPFSNWIASFTEDAMVLGGIWIA
                     LNHPIVFMASLAVFMAFAAWFVIAFARFVRRRLERTETVPAPEGTVR"
     gene            complement(1102..1698)
                     /gene="recR"
                     /locus_tag="VFV55_08155"
     CDS             complement(1102..1698)
                     /gene="recR"
                     /locus_tag="VFV55_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX4944313.1"
                     /translation="MQTPDSLDELIQALRCLPGVGPKSATRMAYHLLQRDHAGAGRLA
                     AALGESLRKIRHCARCNTFTEAEICVLCASPRRDASLLCVVETPADLLMMEQTQSYGG
                     LYFVLMGRLSPLDGIGPREIHLDRLLRRATDGVVKEVILATNFTVEGEATAHYVSEMF
                     AGKGLAVTRIARGLPVGGELEHVDAGTLAQALVERRPA"
     gene            complement(1715..2041)
                     /locus_tag="VFV55_08160"
     CDS             complement(1715..2041)
                     /locus_tag="VFV55_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX4944314.1"
                     /translation="MIKGGLGGLMKQAQQMQENMRKVQESLESIEVEGQSGAGLVKVV
                     MTCKHAVKRVTIDPSLLGEDKDMLEDLVAAAFNDAARRAEATSQEKMAGFTSGLNLPP
                     GMKLPF"
     gene            complement(2038..3711)
                     /gene="dnaX"
                     /locus_tag="VFV55_08165"
     CDS             complement(2038..3711)
                     /gene="dnaX"
                     /locus_tag="VFV55_08165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX4944315.1"
                     /translation="MTYQVLARKWRPKAFEQLVGQPHVVTALANALDTGRLHHAYLFT
                     GTRGVGKTTLARILAKAINCETGVSSRPCGKCRACTEIDSGRFVDMLEVDAATNTKVD
                     EMRELLDNAQYMPVSGRYKVYIIDEVHMLSRHAFNSMLKTLEEPPEHVKFILATTDPQ
                     RLPVTVLSRCLQFNLKALPPLALAGHLKRVLEAEGIPFEDQALALLARAAAGSVRDSL
                     SLADQAIAFGSGKVTAAQVSDMLGAVGGDLVWPLLENIAAADGAGALAEAERISERGL
                     SCDQALQDVAAVLHRVALVQNGAQLPPDDPDSARIASLASRLDAGRVQVMYQVAVLGR
                     RDLPLAPDEIAGFGMAVMRMLSFGTASAAEPVSARGAPVAAPSYGAAGPLAGALREPQ
                     PPAVAAAPAYAPAAPTSIATGAMTAFDGDWTAFVERQSLSGMAGLLARYGEMDSFDDN
                     HLRLVLPEAHRMYAEKSYQDKLKAELAPQFGAGFRLSVRVGETRGRSVAAVRGVEAQQ
                     RIDGATAALESDPFVRDLVEGMGAQVVASSIRPAGEKDSGSTENRRSKP"
     gene            complement(3760..3858)
                     /gene="ffs"
                     /locus_tag="VFV55_08170"
     ncRNA           complement(3760..3858)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFV55_08170"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          640 a         1327 c         1238 g          673 t
ORIGIN      
        1 cgacttgcat gtgtaaagca ttccgccagc gttcaatctg agccaggatc aaactcttca
       61 gttcaatcca tcaaaactca caaagaatca catggattcc aagtgagtac tgcaagatgc
      121 ttcatttccg gcttcccccc atcacctctc ggcaacaggc ttccaccggc gcgcacctac
      181 agcacccacc tctatcggct gtaacttgtt aaagagcgac gggcgtccct tgaagagcag
      241 aatgcctcaa ggaagttggt tgcgggggca ggatttgaac ctgcgacctt cgggttatga
      301 gcccgacgag ctgccagact gctccacccc gcgcccgatt cgaacaaccg gctcacacaa
      361 ataaagatga accgggcgtc gagaggtaaa ttatggcact tttcgcaccc ttgcgtcaac
      421 gaagcgcgcg gccgcgagcc attggacgcg acctctttcc atgaaatcaa ggagctagga
      481 attgagtccg aatgactggc aattgctgca ggcggtggcc ctgggctccc tcctggcgtg
      541 ggcaagcggg ctgcgcctct acctcgtggt gttcgtcctg ggcctggccg cccgcctcgg
      601 ctacctcgac ctgcccgacg ggttggcgat cctctcccac acctatgtca tcgccgcggc
      661 gggtttgctc ctcgccatcg agtttctggc cgacaaggtt cccgggattg acagcgcctg
      721 ggacgccgta cacacgttca taaggattcc tgcgggtgcg ttgctggctg caggagcggc
      781 gggcgacgac ctcacggcga tcacggtggc ggcaggcttg ctgggaggca cgatcaccgc
      841 ggggacgcat ttcgccaagg ccggcggccg cgtggccatc aacgcctcgc ccgagccctt
      901 ctcgaactgg atcgcgtcgt tcacggagga cgcgatggtc ctcggcggca tctggatcgc
      961 gctcaaccac cccatcgtct tcatggcatc gctcgccgtc ttcatggcgt tcgcggcatg
     1021 gttcgtcatt gcgttcgcgc gattcgtgcg ccgtcgcctc gaacgaacgg agacggtgcc
     1081 ggccccggag ggaaccgtcc gctaggcggg gcggcgctcg accaaagcct gggccaacgt
     1141 acccgcgtcg acgtgctcga gttcgccccc gacggggagg ccgcgggcga ttcgcgttac
     1201 ggcgaggccc ttgccggcga acatctccga cacgtagtgg gctgtcgcct cgccctcgac
     1261 cgtgaagttg gtcgcaagga tcacttcctt caccacgcca tccgtggcgc gcctgagcag
     1321 ccggtcgaga tggatctcgc gcgggccgat gccgtccagc ggcgacagcc gtcccatgag
     1381 cacgaaatag aggccgccat agctctgcgt ctgttccatc atgaggaggt cggcaggcgt
     1441 ttccaccacg caaaggagcg aggcgtcccg gcgcggggac gcgcaaagca cgcagatctc
     1501 ggcttccgtg aaggtattgc agcgcgcgca gtggcggatc ttgcgcagcg attcgcccaa
     1561 cgccgctgcg agccgccctg cccccgcgtg gtcgcgctgc aacaggtggt atgccatgcg
     1621 cgtggcggac ttcggaccca cgcccggaag gcagcgcagc gcctggatca gctcatcgag
     1681 cgagtcggga gtctgcatcg gttcgggtga cgattcagaa cggcagcttc atccccggcg
     1741 gcaggttgag ccccgaggtg aagccggcca tcttttcctg gctcgtcgcc tcggccctgc
     1801 gcgccgcatc gttgaacgcg gcggccacga ggtcctcgag catgtccttg tcctcgccga
     1861 gcaggctcgg gtctatcgtc acgcgcttca ccgcgtgctt gcaggtcatc accaccttca
     1921 cgagacccgc gccggactgg ccctcgacct cgatcgactc caggctttcc tggaccttgc
     1981 gcatgttctc ctgcatctgc tgcgcctgct tcatcagccc gccaagaccg cccttaatca
     2041 tggcttcgat ctcctgtttt cggttgaacc ggaatccttt tcgccggcag gccggatgga
     2101 cgacgcgacg acctgcgcgc ccatgccctc cacgaggtcg cgcacgaacg gatcgctctc
     2161 gagcgcagcc gtcgcgccat cgatgcgctg ttgcgcttcc acgccacgaa ccgccgccac
     2221 gcttcggccg cgcgtctctc ccacccggac cgacagccgg aagccggcgc cgaattgcgg
     2281 ggcgagctcc gccttgagct tgtcctggta cgacttttcc gcatacatgc gatgcgcctc
     2341 gggaaggacg agccgcaagt gattgtcgtc gaacgagtcc atttccccat agcgcgccag
     2401 cagcccggcc atgccgctca ggctctggcg ctccacgaat gcggtccagt ccccgtcgaa
     2461 cgcggtcatg gccccggtgg cgattgaagt cggggcagca ggtgcgtaag cgggtgccgc
     2521 ggcaaccgcg ggtggctggg gctcccgaag ggctcccgct agcggacccg ccgcgccata
     2581 ggatggcgcg gcaaccggcg cgccgcgtgc ggaaaccggt tccgccgccg acgccgtgcc
     2641 gaacgaaagc atcctcatca cggccatgcc gaagccggcg atctcgtcgg gcgcgagcgg
     2701 gagatcgcgc cgcccgagga cggcgacctg gtacatcacc tgcacgcgcc ccgcgtcgag
     2761 gcgacttgcg agcgatgcga tccgcgcgga gtccgggtcg tcgggcggga gctgcgcgcc
     2821 attctgcacc agcgccaccc gatgcaggac ggccgcgacg tcctgcaggg cctggtcgca
     2881 cgagagcccc ctttcggaga tccgctccgc ctcggcaagc gcgcccgcgc catcggcggc
     2941 cgcaatattc tcgaggagcg gccacacgag atcccccccg accgcgccga gcatgtcgga
     3001 gacctgggcg gcagtcacct tgccggatcc gaaggcgatc gcctggtcgg cgaggctcag
     3061 gctgtcgcgc acgctgcccg ccgcggccct tgccagcagg gcgagcgcct gatcctcgaa
     3121 ggggatgcct tccgcctcca ggacgcgctt caggtgcccc gcgagcgcca gcggcgggag
     3181 agccttcagg ttgaactgca ggcagcgcga caggaccgtc accggcaatc tctgcgggtc
     3241 ggtcgtcgcg agaatgaact tcacgtgctc gggcggctcc tcgagcgtct tcagcatcga
     3301 gttgaacgca tgccgcgaga gcatgtgcac ttcgtcgatg atgtagacct tgtagcgccc
     3361 cgataccggc atgtactgcg cgttgtcgag aagctcgcgc atctcgtcca ccttggtatt
     3421 ggtggccgcg tcgacctcga gcatgtccac gaaccgcccc gagtcgattt ccgtgcaggc
     3481 gcggcacttc ccgcagggcc gcgacgatac gcccgtctcg cagttgatcg ccttggcgag
     3541 aatgcgcgcg agcgtggtct tgccgacgcc gcgggtgccg gtgaagaggt aggcgtggtg
     3601 cagccggccg gtgtccagcg cattggcgag cgcggtgacc acgtgcggct gtccgacgag
     3661 ctgttcgaac gccttggggc gccacttgcg cgcgagtacc tggtacgtca tggattcgtc
     3721 cggcggaacg gccgcgccac gcgcgaccga aggaatctga aaggcgagcc tgacccccgg
     3781 cacttgaagg aatcgactat ggctgcttcg ttcccgacct gaccaggttc gccgcccttc
     3841 aatgcgggga ggcccgccgg aacggattat cgcacatc
//
LOCUS       DASUEV010000255         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1115085, whole genome shotgun sequence.
ACCESSION   DASUEV010000255 DASUEV010000000
VERSION     DASUEV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115085"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..583)
                     /gene="lptE"
                     /locus_tag="VFV55_08175"
     CDS             complement(92..583)
                     /gene="lptE"
                     /locus_tag="VFV55_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX4944316.1"
                     /translation="MSRPGAALLCLLLAACGFQLRGEAATGLKSLYLSAAVPSPVAAE
                     LRRQLSGGPTKLARDSRAGEAHLRILSESTEKTIQTLTGAGRVFDYQLRLRVGFQVTD
                     ATEKAMIEPTEIEVRRIITSSETAPLAKEVEERLLFEDMRVDAAAQILRRIAATRANA
                     QPR"
     gene            complement(576..3185)
                     /gene="leuS"
                     /locus_tag="VFV55_08180"
     CDS             complement(576..3185)
                     /gene="leuS"
                     /locus_tag="VFV55_08180"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX4944317.1"
                     /translation="MDPQYHPKTLEPAVQKEWADTRAFVAPDASAKPKYFDVGMLPYP
                     SGKLHMGHVRNYSINDALCHYLRMKGFNVLQPMGWDAFGLPAENAAMANGVPPAKWTW
                     ENIAYMKKQLQSLGFAIDWTREVATCSPAYYRWNQWLFLRMREKGIAYKKTGSVNWDP
                     VDQTVLANEQVIDGKGWRTGATVEKREIPMWYLRISQYADELVDALDGLGWPEQVKQM
                     QRNWIGRSYGVEFTLPYEAGTARRMGDGKQGLKVFTTRADTIMGITFAAVAAEHPLAT
                     EAAKGNARLAAFIEECKKGGVMEAELATMEKKGMPTGLFVLHPFTRDKVEVWVGNYVL
                     MGYGEGAVMAVPGHDERDFAFAKQYGLPIRPVIDVDGQAYSTDAWQPWYEAPGVNVNS
                     GKYDGLAQQAAIDAVAADLKAMGLGEKRVQFRLRDWGISRQRYWGTPIPIVHCPACGD
                     VAVPDDQLPVVLPEDLVPDGSGNPLAKSPAFYQCKCPKCGGDARRETDTMDTFVDSSW
                     YFMRYACPDAKTMVDSRADYWMPMDQYIGGIEHAILHLLYARFWTKVMRDMGLVKVDE
                     PFANLLTQGMVLNHIFSRRTDKGGIAYFAPEEVEVKADTEGKIAGATLKADGRPVDYG
                     GIGTMSKSKRNGVDPQDLIDRYGADTARLYVMFASPPTDTILWSDTSVEGSFRFLKRV
                     WKLVHDHLATGGPVAGRAPGELPKELKDLRFKLHRTIDKVGDDYGRRLQFNTAIAAVM
                     ELLNAHTDVKDPSAAARAVAQEVLENAVLLLAPVVPHVATALWAELRPGTRLLDQPWP
                     EVDKAALVQDHVELVVQVNGKLRGHVQVAATASKEDIEKAALASDPAVRFMEGKPARK
                     VVVVPGRLVNIVV"
     gene            3367..>4431
                     /locus_tag="VFV55_08185"
     CDS             3367..>4431
                     /locus_tag="VFV55_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HEX4944318.1"
                     /translation="MQSARRARVIAGPWVFLASFLCASMPLAAVAVDWNGASGVDSNW
                     STAGNWVGGVAPAIANTTVVTFGAAGTTFAPIADAPWTINRLDLTGATPYLLTGGLIT
                     FDGAAPQLNLSGGAHAIANDLSLASDLAFTNAIDLTITGQLSGLGGISKSGPGRLTMA
                     NFLMYSGQTTVNGGTLQIGNGVVGPTGFFVPVVDNATIEFAGSGAAIVLGAGLVGSGD
                     VVVSGGVVQSPGLAMEHAGSTTISGGQLTATVLNNGPLTIAAPGLFESGDSTVGSLAG
                     AGRLDSNGTAVTVGGDGTSTTFSGTLAGLGGLTKVGAGRFTMANFLMYSGQTTVNGGT
                     LQIGNGVVGPTGFFVPVVDNA"
BASE COUNT          655 a         1518 c         1500 g          758 t
ORIGIN      
        1 tccagcgcga cgagcgtttc ggcgccgtgc acggcgtaca acggcgaaag ccccttcttc
       61 gggtgctggg caagctgtcg ggtggcgatc ttcatcgtgg ctgtgcgttg gcgcgcgtgg
      121 cggcgatgcg gcgcaggatc tgcgcggccg cgtcgacgcg catgtcctcg aagagaaggc
      181 gttcctcgac ctctttggcg agcggcgcgg tctccgaaga ggtgatgatg cggcgaacct
      241 cgatctccgt gggctcgatc atcgccttct ccgtggcgtc cgtcacctgg aagcccacgc
      301 gaagccgcag ctggtagtcg aacacgcggc ccgcccccgt gagggtctgg atcgtctttt
      361 ccgtgctctc cgaaaggatg cgcaggtgcg cttcgccggc gcggctgtcg cgcgcgagct
      421 tcgtcggccc cccggaaagc tggcggcgca attcggcggc caccggcgac gggacggcgg
      481 cggagaggta gagcgacttc aggcccgtgg cagcctcgcc gcgcagctgg aacccgcacg
      541 cggccagcaa aaggcacagg agcgccgcgc cgggcctaga caacgatgtt caccaggcgg
      601 ccgggcacca cgacgacctt ccttgccggc ttgccttcca tgaagcgcac cgccggatcg
      661 cttgcgagcg cggccttctc gatgtcctcc ttcgaggcgg tggccgcgac ctgcacgtgg
      721 ccgcgcagct tgccgttcac ctggacgacg agctcgacgt ggtcctgcac cagcgcggcc
      781 ttgtcgacct cgggccaggg ctggtcgagc aggcgcgtgc cggggcgaag ctcggcccac
      841 aacgcggtgg cgacgtgcgg gacgacgggc gcgaggagca gcaccgcatt ctcgagcacc
      901 tcctgggcca ctgcccttgc cgcggcggac ggatccttca cgtcggtgtg cgcgttgagc
      961 agctccatca ccgcggcgat ggcggtgttg aactgcaggc gccgcccgta gtcgtcgccc
     1021 accttgtcga tggtccggtg cagcttgaag cgcaggtcct tcagctcctt cgggagttcg
     1081 cccggggcgc ggcccgcaac gggaccgccc gtcgcgagat ggtcgtgcac cagcttccac
     1141 acgcgcttga ggaagcggaa ggagccctcg acggaggtgt cggaccagag gatcgtgtcc
     1201 gtgggcgggc tcgcgaacat cacgtagagc cgcgccgtgt ccgcgccgta gcggtcgatg
     1261 aggtcctgcg gatcgacgcc attgcgcttg gacttcgaca tcgtgccgat gccgccgtag
     1321 tcgacgggcc tgccgtcggc cttgagcgtg gcgccggcga tcttgccctc cgtgtcggcc
     1381 ttaacctcca cctcctccgg cgcgaagtag gcgatgccgc ccttgtcggt gcgccggctg
     1441 aagatgtggt tgagcaccat cccctgcgtg aggaggttcg cgaacggctc gtccaccttc
     1501 accagcccca tgtcgcgcat caccttggtc cagaagcgcg catagagcag gtgcaggatc
     1561 gcgtgctcga tgccgccgat gtactggtcc atcggcatcc agtagtcggc gcgcgagtcc
     1621 accatcgtct tcgcgtcggg gcacgcatag cgcatgaagt accacgacga atcgacgaag
     1681 gtgtccatcg tgtccgtctc gcgcctcgcg tccccgccgc acttcgggca cttgcactgg
     1741 tagaacgcgg ggctcttcgc gagcgggttg ccgctgccgt ccgggacgag gtcttccggc
     1801 agcaccacgg gaagctggtc gtcgggcacc gcgacgtcgc cgcacgccgg gcagtggacg
     1861 atcgggatgg gcgtgcccca gtagcgctgg cgcgagatgc cccagtcgcg aagccggaac
     1921 tgcacgcgct tttctcccag gcccatcgcc ttcaggtcgg cagccaccgc gtcgatcgcg
     1981 gcctgctgcg caagcccgtc gtactttccg gaattgacgt tcaccccggg cgcttcgtac
     2041 cacggctgcc acgcgtcggt ggagtacgcc tgcccatcca cgtcgatcac cggcctgatg
     2101 ggcaggccgt attgcttcgc gaaggcgaaa tcgcgctcgt cgtggcccgg cacggccatc
     2161 accgcgcctt cgccgtagcc catgagcacg tagttgccga cccacacctc gaccttgtcc
     2221 cgggtgaagg gatgcaggac gaagaggccc gtcggcatgc ccttcttctc catggtggcc
     2281 agctcggcct ccatcacgcc gcccttcttg cactcctcga tgaaggcggc gagcctcgcg
     2341 ttgcccttcg cggcctccgt ggcaagcgga tgctccgcgg ccaccgccgc gaaagtgatg
     2401 cccatgatcg tgtcggcgcg cgtggtgaag accttgaggc cctgcttgcc gtcccccatg
     2461 cggcgcgcgg tcccggcctc gtagggcagc gtgaactcga cgccgtagga gcggccgatc
     2521 cagttgcgct gcatctgctt cacctgctcg ggccagccca ggccgtcgag ggcgtcgacg
     2581 agctcgtcgg cgtactgcga gatgcgcagg taccacatgg ggatctcgcg cttctccacc
     2641 gtcgccccgg ttcgccagcc cttgccgtcg atcacctgct cgttggccag cacggtctgg
     2701 tccaccgggt cccagttcac cgaacccgtc ttcttgtagg cgatgccctt ctcgcgcatg
     2761 cgcaggaaca gccactggtt ccaccggtag taggcgggcg agcaggtcgc cacctcgcgc
     2821 gtccagtcga ttgcgaagcc cagcgactgc agctgcttct tcatgtaggc gatgttctcc
     2881 cacgtccact tcgcgggcgg cacgccgttg gccatggccg cattctcggc gggcaggccg
     2941 aacgcgtccc agcccatggg ctgcaggacg ttgaagccct tcatgcgcag gtaatggcag
     3001 agcgcgtcgt tgatcgagta gttgcgcacg tgccccatgt gcagcttgcc cgacgggtaa
     3061 gggagcatgc ccacgtcgaa atacttgggc ttcgcggacg cgtcgggcgc gacgaaggcc
     3121 ctcgtgtcgg cccactcctt ctgcacggcg ggctcgaggg tcttcgggtg gtattgggga
     3181 tccattgcga tctgcgctcg tgaattgcgt tgtgcgatgc ggcaattata gcagcggggt
     3241 ccatccaacc ccttgatttg gcgcgcgcta tcgcggcctg gaccttgtgc gccccgattc
     3301 ttttcccggc cccgaccccg tcttgtacta tccgggcgag aagaaccagg aaccagggag
     3361 aaaaaaatgc agagcgcgcg ccgggcccgc gtcatcgcgg ggccgtgggt cttcctcgca
     3421 tcattcctgt gcgcctcgat gcccctggcg gcggtagccg tcgactggaa cggcgccagc
     3481 ggcgtcgaca gcaactggag caccgccggc aactgggtcg gcggcgtcgc acccgcaatc
     3541 gccaacacca cggtcgtcac tttcggcgcg gccggcacga cgttcgcgcc gatcgccgat
     3601 gcgccgtgga cgatcaaccg gctggacctc accggggcga cgccctacct gcttaccgga
     3661 ggactcatca ccttcgatgg cgctgcgccg caactgaacc tgtccggcgg tgcccacgcc
     3721 atcgcgaacg accttagcct cgcctcggac ctcgcgttca cgaacgcgat cgatctcacg
     3781 attaccggcc agctgagcgg cctgggcggg atctcgaagt ccggcccggg ccgcctcacg
     3841 atggccaatt tcctgatgta cagcggccag acgacggtga acggcggcac gctgcagatt
     3901 ggcaacgggg tggtggggcc gacgggcttc ttcgtgcccg tcgtcgacaa cgcgacgatc
     3961 gaatttgccg gctcgggcgc cgccatcgtc ctgggcgccg ggctcgtggg gtcgggcgac
     4021 gtggtcgtat ccggcggcgt cgtccagagt cccgggctgg ccatggagca cgcgggctcg
     4081 acgacgatct ccggaggcca gctcactgcg actgtactga acaacgggcc gctcacgatc
     4141 gccgcgcccg ggctgttcga gagcggcgac agcacggtgg gctcgctcgc cggcgcggga
     4201 aggctcgact cgaacggcac cgcggtcacg gtgggcgggg acggcaccag cacgaccttc
     4261 agcggcacgt tggccggcct cggcggcctc acgaaggtgg gcgcgggccg gttcacgatg
     4321 gccaatttcc tgatgtacag cggccagacg acggtgaacg gcggcacgct gcagattggc
     4381 aacggggtgg tggggccgac gggcttcttc gtgcccgtcg tcgacaacgc g
//
LOCUS       DASUEV010000256        14423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_514590, whole genome shotgun sequence.
ACCESSION   DASUEV010000256 DASUEV010000000
VERSION     DASUEV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514590"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..3032
                     /locus_tag="VFV55_08190"
     CDS             246..3032
                     /locus_tag="VFV55_08190"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX4944319.1"
                     /translation="MVTRLFSRTPAHEHPEASQRILGLEHLSPESAELAALVTGDPAP
                     EVRAAAAARCADPGALAAALGKETDAAVRTALAAALGRVLAAMADGAAARAFLEAPAC
                     TDAIRADVARQAHDADRRLAAIAGIRDEPLLVELALAGGHAETRLAAAECVRTPEGLS
                     ALVGAARNKDRGVTRLAKQRLEAMKDREGQASEADAILAQLEALAGTPGPILTAVVDL
                     NRRWQVLDMHEDAARLERCDAARRALQARFDREQAEQAARAKFERHLGEWIAALAAPD
                     GPEALAGLRTQLEQLREEAQALGDPAAPARLADAQARLDGWAQELQALAGAEALVVEA
                     ERLAADTSVDNAQLPERWQALDRAIRTPALTRRFEAALVIVEQRRLAQAQVAREEANA
                     TRNRIHALLHAAEQALAAGQLHATRAAVDEMKALKADAGALPKPTQQRMGRVGQQLGE
                     LERWESFGQQTARIQLCERAEAIATQALDAARTAAEVKKLREEWKTLDAQHAGVPRAL
                     WERFDGACEKAYAPAARHFAELAAQRKAARKQRDEFIAAAAAHVPTLLGETPDLRAIE
                     RWLRETDRAWREGELGSVDPGAWKKLDARLKEALAPLRAALSSAREQAKTGRLALIAE
                     VEALAPRAMEREAPSLVKAVQARWQEHSKANPLAQRDERALWERFRAACDAVFEARHA
                     KRKEEDGRKSEGRRALEDTCAQLEQLARQTEKDEKEVRGALRDLQDRWRAGARDADPA
                     LRALEGRFRSAVKSVEGSLSARVRSREAAVWKTLAGKERLCEELDAAVLSGGDAALPD
                     SVRDRWAALPELPPAWEKKMLARREAALAALSDGAALGEYRPRIERGTLARRESLLEL
                     EMALGLDSPAEFQALRLALQVKLLKERFGGGATAAGGTTGDRLLAWCAQPGVIEGRDR
                     ERGDRVFSAMGQAG"
     gene            3037..3903
                     /locus_tag="VFV55_08195"
     CDS             3037..3903
                     /locus_tag="VFV55_08195"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX4944320.1"
                     /translation="MLELPNPFAGEGGALRLLEPPDCDREAIVDRVLSGTYAKPFVIE
                     DEGLRSLYFTRAFIQSEMRLADPYALEFAYTRKMMGFLLFLHEPRAVLMLGLGGGSLA
                     KYCHRHLPAARITAVELDPQVIAFRDQFLVPQDDARFRVIEDDAAGYVAQCEEHFDVV
                     MIDAFDRDGVAPSLCTREFYEDVRDLLPRKGLVVANLVGEKAERLAHLEMMRTVFGGN
                     VILLPVADDGNYVAFAFRDPAFEPRWRWIDDQARSMRARYGLDFPKFAGKLERSRKLG
                     YLRREMNQARSR"
     gene            complement(3926..5536)
                     /locus_tag="VFV55_08200"
     CDS             complement(3926..5536)
                     /locus_tag="VFV55_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX4944321.1"
                     /translation="MQVIDLAPVIVLTAIGGVLGRWSRMPLPIFLVVVGAVASFIPGL
                     DAVKVDPEVFLLLFIPPLLFADARVLPRRDLLHVLRPVLLLALGLVVLTVIAVGYFIH
                     WLIPSMPLAVAFTLGAIVSPTDAVATVATTATVPLPNRVTHIVNAESLLNDATGLVAF
                     KLAVVTAVAASYVSMYEITGQFVILSGGGLFVGIVIEWIGRKLREQLIILKAEDPVVQ
                     TLLTVLVPYAAYIVAEALHVSGILAVVASGLWAGGQEVKGLSVESRRHAREVWRMIGW
                     VFNGLVFVLLGLQLRQMVQGVANHDVVHLAYWAMALWVLLMLLRFGWVWASAHVRFRL
                     HWGWAGGQEGPDPKRMFLVGWAAVRGSITLATALSIPLLTGAGDPFPERDLVVFLAAA
                     TIILTLALNGLSLPWFIRRLRAQDDDRGEIEESAARTELARAAIAVVEPAAERFPDPE
                     DKAFARALLGRYQGKVDLRESSPEQAESRATHIATARRLRLDAIAAERERLHELLKSD
                     AINDATALLIEEELDEHEVVSSADPDRG"
     gene            5661..6884
                     /locus_tag="VFV55_08205"
     CDS             5661..6884
                     /locus_tag="VFV55_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4944322.1"
                     /translation="MDTVEAVYRAESRRVLSTLIRLLGDFDLAEEALHEAFVAAVEQW
                     RVEGVPANPRAWLVSTGRFKAIDRIRRNARFDTSLDDIADRLEAIPAPDASEEHGGVE
                     DDRLRLIFTCCHPALSPDAQVALTLREVCGLTTEEIAHAHLATPATVAQRIVRAKNKI
                     RDARIPYEVPGRAELAGRLDSVLRVIYLVFNEGYLASSGDALLRHDLSGEAIRLGRLL
                     VELLPEPEAVGLLALMLLHESRRAARTTVAGEIVLLADQDRSLWDRERIADAVRLVEG
                     ALSTQRFGPYTLQAAIAAVHAEAPSPGATDWAEIVGLYDALLRLDPSPVVELNRAIAV
                     AMRDGPAAGLALVDALLARGELDDYHLAHSARADLCRRLGNRPEARRSYHRALALTRQ
                     ASERRFLEGRLAELE"
     gene            6967..7356
                     /locus_tag="VFV55_08210"
     CDS             6967..7356
                     /locus_tag="VFV55_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4944323.1"
                     /translation="MKYLCLIYLNENQLAALPEAEMNTLNARHLELNDELLTSGQFIE
                     AEALQSAAATTCVRVRNGKTTVTDGPFAEAKESVAGFYFIEARDLNEAIRVASRFPSA
                     PLGTVEVRPCRDLIVDGVQHKASRKSR"
     gene            7386..7811
                     /locus_tag="VFV55_08215"
     CDS             7386..7811
                     /locus_tag="VFV55_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX4944324.1"
                     /translation="MRFLVMVKATKDSEAGVMPDEKLLADMGRFNEELVKAGVMLAGE
                     GLQPSSKGARVRFSGSKRTVIDGPFPETKELVAGFWMWKVKSLEEAIDWVKRCPSPHP
                     TDCEIEIRQVFEAEDFGAEFTPELREAEERLRKQAANNR"
     gene            7824..8351
                     /locus_tag="VFV55_08220"
     CDS             7824..8351
                     /locus_tag="VFV55_08220"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4944325.1"
                     /translation="MLKTIAIIVGLAVVVILAAAANKPDTFRVQRSASIKAPPEKIFP
                     LLTDFRNWAAWSPWEKRDPALRRTYAGPASGTGASYAWEGNKDVGSGRMEIAEAAPPS
                     EVRIKIDFAQPFEAHNIVEFRLTPEGEATRIAWAMHGPQTFPGKLMSVFFSIDNMIGR
                     DFEAGLASLKSVAEN"
     gene            8361..8774
                     /locus_tag="VFV55_08225"
     CDS             8361..8774
                     /locus_tag="VFV55_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4944326.1"
                     /translation="MQVQPYLFFDGRCEEAIEFYRKALGAEVTMLMRVKDSPDPPPPG
                     MYPPDSGNKVMHASLRIGEATVMASDGRCQGKPVFQGFSLSLDARTEADADRLFAALA
                     DGGTVQMPLGKTFFSPRFGMVADRFGVSWMVIVLP"
     gene            complement(8812..9573)
                     /locus_tag="VFV55_08230"
     CDS             complement(8812..9573)
                     /locus_tag="VFV55_08230"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944327.1"
                     /translation="MNVHAVLAIYRFELARFRRTLWQSLVTPVIITSLYFVVFGAAIG
                     PRMTQVDGVPYGAFIVPGLIMLSIFTESLNNASFGIYLPKFAGTIYELLSAPVSAFEI
                     VLAYVGAAATKSILLGAVILATANLFIAVRIDHPVWMVAFLVLVAATFSLFGFILGIW
                     ANTFEQLNFVPMLVVTPLTFLGGAFYSIDMLPPAWRTFSLFNPIVYLISGYRWSFYGV
                     SDVSVAVSLAMTLAFFVACLAVVAWIFRTGYRLKS"
     gene            complement(9570..10535)
                     /locus_tag="VFV55_08235"
     CDS             complement(9570..10535)
                     /locus_tag="VFV55_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944328.1"
                     /translation="MTAAPRPPVISIAGLTKSYASGLQALKGVDLEIRRGEIFALLGP
                     NGAGKTTLISIVCGIVTPSTGTVRVDGHDIVSGYRLARSKIGLVPQELHTDMFETLWA
                     TMCFSRGLFGRAPDPAHLEKVLRELSLWEKRNDKIMTLSGGMKRRVLIAKALSHEPEI
                     LFLDEPTAGVDVELRRDMWALVRRLRDAGVTIILTTHYIEEAEEMADRIGVISKGELI
                     VVEEKARLMKKLGKKQLSLHLLQPLASVPAALADWNLALIEEGSVLEYTFDNEERTAV
                     PALLRRLDDLGIGFADLNTRQSSLEDIFVSLVSDRAGSPPGRKFP"
     gene            complement(10674..12326)
                     /gene="groL"
                     /locus_tag="VFV55_08240"
     CDS             complement(10674..12326)
                     /gene="groL"
                     /locus_tag="VFV55_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110680.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX4944329.1"
                     /translation="MAAKDVKFKEHARVRMMTGVNILADAVKVTLGPKGRNVVLERSF
                     GSPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDIAGDGTTTATVLAQSIATE
                     GMKYVAAGMNPMDLKRGIDKAVIATVEELKKISKPCTTSKEIAQVGAISANADENIGK
                     IISDAMDKVGKEGVITVEDGKSLDNELDIVEGMQFDRGYLSPYFINNPDRQIAVLEDP
                     FVLLHDKKISNIRDLLPVLEQVAKAGRPLLIVAEDVDGEALATLVVNNLRGILKTCAV
                     KAPGFGDRRKAMLEDIAILTGGTVIAEELGLKLENVQLKDLGKAKRIEIAKEDTTIID
                     GSGKEDQIKARVSIIRSQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRAKEAVSKVKGDNADQEAGIKIVLRALEQP
                     LREIARNAGDEPSVVVNKVMEGKGNYGYNAQTGEYGDMVSMGVLDPTKVTRCALQNAA
                     SVAALMLTTDAMVAELPKDDKGGMPGGMGGMGGMGGMGDMDM"
     gene            complement(12361..12648)
                     /gene="groES"
                     /locus_tag="VFV55_08245"
     CDS             complement(12361..12648)
                     /gene="groES"
                     /locus_tag="VFV55_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX4944330.1"
                     /translation="MKLRPLHDRVIVKRLEEERKTASGIVIPDTVAEKPDQGEVLAVG
                     PGKKNEEGKLSPVDVKVGDKILFGKYSGTTVKIDGEELLVMREEDIMAVVA"
     gene            complement(12986..13618)
                     /locus_tag="VFV55_08250"
     CDS             complement(12986..13618)
                     /locus_tag="VFV55_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4944331.1"
                     /translation="MKLLDCIESQLIANRLPLVGVSVAAVPYANTPVVLTLHWHGFVE
                     TRLANVTDANVIAYQPVPSSALQVNDRWDRFEELENAVLDVAWELGSWDLARREAPPF
                     IRPGSSAQESLECMGAFGNLPIAIEGVPPIVAEVPDGDELIDAAGRSGYVQWMFRPVR
                     GGVWADVAEDMTLEQGGYRNPPCPFLAAPTKPGASRRVVYQFGRSDRLVM"
     gene            14067..14345
                     /locus_tag="VFV55_08255"
     CDS             14067..14345
                     /locus_tag="VFV55_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944332.1"
                     /translation="MILDSLTAHWNAHGSGGLLAPGTRVALDTKGPSIVTADARGFLA
                     SVEIRTLFTALCHLSADGPGSMPRDSLTALATEAANSLAAQVNRLVRD"
BASE COUNT         2367 a         4863 c         4943 g         2250 t
ORIGIN      
        1 tcgaagggcg ataactgctt gacgcactgt atccctgcaa cgcgaggcat acaacgcgcc
       61 gggctgacgc agatcaagcc tgcgcgaaat tgagggcggc gaggtcggga ttccccggcg
      121 cgatccgggc attcgtcgtc cggtgttgcc attgcagatg gccgtcgggt gccccacagg
      181 gattatcatt cgggtagccg catcgtcccg catgcgccct gcccgctccg atacccgtat
      241 ccatcatggt cacccggctc ttctcacgca cccccgctca cgagcatccc gaggcctccc
      301 agcggatcct gggcctggag catctgtccc cggaatccgc cgaactggcc gcactcgtca
      361 ccggcgatcc cgcgccggaa gttcgtgccg cggccgccgc gcgttgcgcc gatccggggg
      421 cgcttgcggc tgccttggga aaggaaaccg acgccgcggt tcgcaccgca ctcgcggccg
      481 cgctgggccg cgtgctcgcc gcgatggcgg acggcgccgc cgcgcgggcg ttcctcgagg
      541 cccccgcctg caccgacgcg atccgtgccg acgtggcgcg ccaggcccac gatgcggacc
      601 ggcgccttgc cgccatcgcc ggcattcgcg acgagcccct cctcgtcgag ctggcgcttg
      661 ccggcgggca cgcggaaacc cgcctcgcgg ccgccgagtg cgtccggacg cccgagggcc
      721 tgagcgcgct cgtcggtgcg gcccgcaaca aggaccgcgg cgtgacccgg ctcgcgaagc
      781 agcgcctcga ggcgatgaag gaccgcgagg gccaggcgag cgaggccgat gcgatcctcg
      841 cgcagctgga ggcgctcgcc ggcacgcccg gccccatcct cacggcggtg gtcgacctga
      901 atcgccgctg gcaggtcctg gacatgcacg aggacgccgc ccggctcgag cgctgcgacg
      961 cggcgcgccg cgcgctgcag gcgcgcttcg accgcgagca ggccgagcag gcggcgcgcg
     1021 cgaagttcga acgccacctg ggcgagtgga tcgcggccct cgcggcgccc gacgggccgg
     1081 aggcgctggc cggcctgcgc acccagctcg agcagcttcg cgaggaggcg caggcgctcg
     1141 gggacccggc cgcgccggcc cgtctcgccg acgcgcaggc ccgcctcgat ggctgggcgc
     1201 aggaattgca ggcactcgcc ggcgccgagg cgctggtcgt cgaggccgaa cgcctggccg
     1261 cggacacttc ggtggacaat gcgcagcttc ccgaacgctg gcaggcgctg gaccgcgcca
     1321 tccgcacgcc ggcgttgacg cggcgcttcg aggcggcgct cgtgatcgtc gagcagcgcc
     1381 gcctcgccca ggcacaggtc gcgcgcgagg aggccaacgc gacgcgcaac cgcatccacg
     1441 cgctgctgca cgcggcggaa caggcgctgg ccgccgggca attgcacgcg acgcgcgctg
     1501 cggtggacga aatgaaggcc ctcaaggccg acgccggggc attgcccaag ccgacccagc
     1561 agcgcatggg ccgcgtcggg cagcagctcg gcgagctgga acgctgggag tcgttcggcc
     1621 agcagaccgc gcgcatccag ttgtgcgagc gcgcggaggc catcgccacg caggcgctcg
     1681 acgccgcgcg cacggccgcc gaggtgaaga aacttcgcga agagtggaag acgctcgacg
     1741 cgcagcacgc cggcgtgccg cgcgcgttgt gggagcgctt cgacggcgcg tgcgagaagg
     1801 cgtacgcgcc cgctgcccgg catttcgcgg agcttgcggc gcagcgcaag gcggcgcgca
     1861 agcagcgcga cgaattcatc gctgccgcgg ccgcgcacgt gcccaccctg ctcggcgaaa
     1921 cgccggacct gcgcgccatc gaacgctggc tgcgcgaaac cgaccgcgcg tggcgcgagg
     1981 gggaactggg cagcgtggac ccgggcgcgt ggaagaagct cgacgcccga ctcaaggagg
     2041 cgctcgcgcc gctgcgcgcg gcgttgtcgt cggcgcgcga gcaggcaaag acggggcgcc
     2101 tggcgttgat cgcggaagtc gaggcactgg cgccgcgcgc gatggagcgc gaggcgccct
     2161 cgctcgtgaa ggccgtccag gcgcgctggc aggagcactc caaggcgaat ccgctcgcgc
     2221 agcgcgacga acgcgcgctg tgggaacgct tccgcgccgc ctgcgacgcc gtgttcgagg
     2281 ctcgccacgc caagcggaag gaggaggacg gccgcaagtc cgagggccgc cgcgccctcg
     2341 aggacacctg cgcgcaactg gagcagctcg cgcggcagac ggagaaggac gagaaggaag
     2401 tgcgcggggc gttgcgcgac ctccaggatc gctggcgcgc gggtgcccgc gacgccgacc
     2461 cggcgctgcg cgcgctcgag ggacgcttca ggagcgcggt gaagtccgtg gaaggctcgc
     2521 tctcggcccg cgttcgctcc cgcgaggccg cggtgtggaa gacgctcgcc gggaaggagc
     2581 gcctgtgcga ggagctggac gcagccgtgc tttccggcgg cgatgccgcc ttgcccgact
     2641 cggtgcgcga ccggtgggcc gccctgcccg aactgcctcc cgcctgggag aagaagatgc
     2701 tcgcgaggcg cgaagcggcg ctcgccgcac tttcggatgg cgccgccctg ggcgaatacc
     2761 ggccgcgcat cgaacgcggc acgctggcgc gacgcgagag cctcctcgaa ctcgagatgg
     2821 ccctgggcct cgacagcccc gccgagttcc aggcgctgcg gcttgccctg caggtgaagc
     2881 tcctcaagga acgcttcggc ggcggcgcaa cggctgcggg cggcacgacg ggcgatcgac
     2941 tcctcgcctg gtgcgcccag ccgggagtga tcgaaggccg cgaccgcgag cgcggcgacc
     3001 gcgtgttctc cgcgatggga caggcaggct gacgcgatgc tggaactgcc gaatcccttc
     3061 gccggggaag gtggcgcgtt gcggctgctg gagccgccgg actgcgaccg cgaggcgatc
     3121 gtcgaccggg tcctgtcggg cacctacgcg aagcccttcg tgatcgagga cgagggcctg
     3181 cgctcgctct acttcacgcg cgccttcatc cagagcgaaa tgcggctggc ggatccctac
     3241 gccctcgaat tcgcgtacac gcgcaagatg atgggcttcc tgctgttcct gcacgagccg
     3301 cgcgcggtgc tgatgctggg cctgggaggc ggctcgctcg ccaagtactg ccaccgccac
     3361 ctgccggcgg cgcgcatcac cgcggtcgag ctcgacccgc aggtcatcgc cttccgcgac
     3421 cagttcctcg tcccgcagga cgacgctcgc ttccgggtga tcgaggatga tgcggccggg
     3481 tatgttgcgc agtgcgagga gcacttcgac gtcgtcatga tcgacgcctt cgaccgcgac
     3541 ggcgtcgccc cgtcgctgtg cacgcgcgaa ttctacgagg acgtgcgcga cctgctgccg
     3601 cgcaagggtc tggtggtggc caacctcgtc ggcgagaagg cggagcgcct cgcgcacctc
     3661 gagatgatgc gcaccgtgtt cggcggcaac gtgatcctgc tgcccgtggc ggacgacgga
     3721 aactacgtcg ccttcgcgtt ccgcgacccc gcgttcgagc cgcgctggcg ctggatcgac
     3781 gaccaggcca ggtccatgcg cgcccgctac ggtctcgatt tcccgaagtt cgccggcaag
     3841 ctggagcgca gccgcaagct gggctacctg cggcgcgaga tgaaccaggc gcgatcgcgc
     3901 tgaggcagcc cgggccgccg gcgcctcacc cccggtcggg atcggcggac gacacgactt
     3961 cgtgctcgtc gagctcctcc tcgatgagca gggccgtcgc gtcgttgatg gcatcggact
     4021 tgagcagttc gtgcagccgc tcgcgttccg cggcgatggc gtcgaggcgc agccgccttg
     4081 cggtggcgat gtgcgtcgcg cgcgattcgg cctgctccgg cgagctttcg cgtaggtcca
     4141 ccttgccctg gtagcgcccg agcaacgccc tggcgaaagc cttgtcctcg gggtccggaa
     4201 accgttcagc cgcgggctcc accaccgcga tcgccgcgcg tgcgagctcc gtccgcgccg
     4261 cgctctcctc gatctcgccc cggtcgtcgt cctgggcgcg gagcctacgg atgaaccagg
     4321 gcagcgaaag gccgttgagc gcgagggtca ggatgatggt ggctgcggcc aggaacacca
     4381 ccaggtcgcg ttcgggaaag ggatcgccgg caccggtgag aagcggtatg gagagtgccg
     4441 tggcgagcgt gatggagccc ctcacggccg cccatcccac caggaacatc cgtttcgggt
     4501 ccggcccctc ctgcccgccc gcccagcccc agtggaggcg aaagcgcacg tgggcggaag
     4561 cccacaccca gccgaagcgc aacagcatga gaagcaccca cagcgccatc gcccagtacg
     4621 caaggtgcac gacgtcgtgg tttgcgacgc cctgcaccat ctggcgcaac tgcaggccga
     4681 gcaggacgaa cacgagcccg ttgaacaccc agccgatcat ccgccacacc tcgcgggcgt
     4741 ggcgccggct ttccaccgag agtcccttca cttcctgccc gccggcccag agacccgagg
     4801 ccacgaccgc gaggattccc gagacgtgca gcgcttccgc gacgatgtag gcggcgtagg
     4861 gcacgagcac cgtgaggagg gtctgcacca ccggatcctc ggccttgagg atgatgagtt
     4921 gctcgcgcag cttgcggccg atccactcga tgacgatgcc gacgaacagg ccgccgccgg
     4981 agaggatcac gaactggccg gtgatctcgt acatcgacac gtaactcgcc gcaacggcgg
     5041 tcacgaccgc gagcttgaag gcgacgaggc cggtggcgtc gttgagcagg ctctcggcgt
     5101 tgacgatgtg cgtgacgcgg ttgggcaagg gcaccgtcgc ggtggtcgcg accgtggcca
     5161 ccgcgtccgt gggcgacacg atggccccca gcgtgaacgc caccgccagc ggcatcgacg
     5221 ggatcagcca atggatgaag tagccgactg cgatcaccgt gagcacgacg aggcccaatg
     5281 ccagcagcag caccggcctg aggacgtgca gcaggtcgcg tctcggcagc acccgggcat
     5341 ccgcgaagag cagcggcgga atgaacagca ggaggaagac ctccggatcg accttgacgg
     5401 cgtcgagccc cggaatgaac gaggcgactg cgccgacgac gaccaggaag atcggcaggg
     5461 gcatccgcga ccagcgcccg aggacgcctc cgatcgcggt gaggacaatg acgggcgcga
     5521 ggtcgatgac ttgcatggca cttcgggggg gcgggcagga tgtgccgcgg cagggggaaa
     5581 tgatggcctt tccccgccga ccgcgggttg cgcaagcgca aaatgccggt tttcgtcgcc
     5641 agcccgcgag ccgaccccga atggataccg tcgaagccgt ttaccgcgcc gagtcgcgcc
     5701 gggtcctctc gaccctgatc cgcctgcttg gcgatttcga cctcgccgag gaggcgctgc
     5761 acgaggcctt tgtcgccgca gtggagcaat ggcgggtcga aggcgtgccc gccaatccgc
     5821 gcgcctggct cgtgtccacg ggccgcttca aggccatcga ccggatacgg cgcaatgccc
     5881 gtttcgacac gtcgctcgac gacatcgcgg accggctgga agccatcccg gcgcccgatg
     5941 cttccgaaga gcacggcggc gtcgaggacg accggctcag gctgatcttc acgtgctgcc
     6001 accccgcact gtcgcccgac gcgcaggtgg ccctcaccct gcgcgaggtt tgcggcctca
     6061 ccacggagga gatcgcgcac gcccatctcg ccacgccggc cacggtcgcg cagcggatcg
     6121 tgcgcgccaa gaacaagatc cgcgatgccc gcatcccgta cgaggtgccg gggcgcgcgg
     6181 agcttgcggg ccggctggac agcgtgctgc gcgtcatcta cctcgtcttc aacgagggct
     6241 acctcgcttc atcgggcgac gcgctgctgc gccatgatct ttcgggcgag gcgatccgcc
     6301 tcggccgcct gctcgtggag ctgctgccgg agccggaggc ggtcgggctc ctcgcgctca
     6361 tgctgctgca cgaatcgcgg cgcgcggcgc gcacgacggt ggcgggcgag atcgtattgc
     6421 tcgccgacca ggaccgttcg ctgtgggacc gggagcggat cgccgacgcc gtcaggctcg
     6481 tggaaggggc actttccacg cagcgcttcg gtccctacac gttgcaggcg gcgattgcgg
     6541 cggttcacgc ggaggctccc tcgccggggg ccacggactg ggccgagatc gtcggccttt
     6601 acgacgcgct cctgcgcctc gatccgtcgc ccgtggtgga gctcaatcgc gccatcgcgg
     6661 tggccatgcg cgacgggccc gcggcggggc tggcgctcgt ggatgcgctt ctcgcgcgcg
     6721 gcgagctcga cgattatcat ctcgcccact cggcccgggc ggacctgtgc cggcggctgg
     6781 gaaacaggcc cgaggcgcgc aggtcctatc accgggccct ggcactcacc cgccaggcct
     6841 ccgagcgacg tttcctggaa gggcgcctgg cggaactcga atgattttcg cgtgcggttg
     6901 tcgaatcggc cccccgccaa tcgactaccc ggtgtggcag gccgcgcaat cccgatcgag
     6961 gagaccatga aatacctttg cctgatctac ctgaacgaga accagctggc cgcgttgccc
     7021 gaggcggaaa tgaacacgct gaacgcccgg cacctcgagc tcaacgacga attgctcacc
     7081 agcggccagt tcatagaggc ggaagccctg cagagcgccg ccgcgacgac ctgcgtgcgc
     7141 gttcgcaacg gcaagacaac ggtcaccgac ggccccttcg ccgaggcgaa ggagtcggtg
     7201 gcagggttct acttcatcga ggcgcgcgac ctgaacgagg cgatccgggt ggcgtcgcga
     7261 ttccccagcg cgcccctggg cacggtcgaa gtgcgcccct gccgcgacct catcgtcgat
     7321 ggcgtgcaac acaaggcctc gcgcaagtcc cgttgattcc caaccaaccg aaaggagatt
     7381 cgtccatgcg cttcctggtg atggtcaagg cgacaaagga ttccgaggcg ggcgtcatgc
     7441 ccgacgagaa gctgctggcc gacatgggcc ggttcaacga ggagctggtg aaagccggcg
     7501 tcatgctcgc cggcgaaggg ctgcagccaa gctcgaaggg cgcgcgcgtg cgcttctcgg
     7561 gctcgaagcg aaccgtcatc gacggtccgt tcccggagac caaggaactc gtcgccggct
     7621 tctggatgtg gaaggtgaag tcgctggagg aggcgatcga ctgggtgaag cgctgcccct
     7681 ccccccaccc gacggattgc gagatcgaga tccgccaggt gttcgaggcg gaggacttcg
     7741 gcgcggaatt caccccggag ctgcgcgagg ccgaggagcg cctgcgcaag caggcggcga
     7801 acaatcggta aggagcagcg accatgctca agaccatcgc cataatcgtc gggctcgccg
     7861 tcgtggtgat tcttgccgcc gccgcgaaca agccggacac ctttcgtgtc cagcgttccg
     7921 caagcataaa ggccccgccg gagaagatct ttccgctgct caccgacttc cggaattggg
     7981 ccgcgtggtc gccgtgggag aagagggacc cggcgctcag gaggacatac gccggcccgg
     8041 caagcggcac gggcgcgagc tacgcgtggg aaggcaacaa ggatgtgggt tccgggcgca
     8101 tggagatcgc cgaggccgcg ccgccttccg aagtgcggat caagatcgat ttcgcgcagc
     8161 ccttcgaggc gcacaacatc gtcgagttca ggctcacgcc ggagggcgag gccacgagga
     8221 tcgcctgggc gatgcacggg ccgcagacct tccccgggaa gctgatgagc gtcttcttca
     8281 gcatcgacaa catgatcggc agggatttcg aggccggcct ggccagcctc aagtccgtcg
     8341 cggaaaactg aggagcaacc atgcaagtcc agccctacct gttcttcgac ggccgctgcg
     8401 aggaggcgat cgagttctac cgcaaggccc tcggcgccga agtgacgatg ctcatgcgcg
     8461 tgaaggatag ccccgatccc ccgccccccg gcatgtaccc gccggactcc gggaacaagg
     8521 tgatgcacgc cagcctgcgc atcggagagg cgacggtgat ggcttccgac ggccggtgcc
     8581 agggcaagcc tgtgttccag ggattctcgc tttccctcga cgcgcgaacc gaggccgacg
     8641 ccgatcgcct gttcgccgcc ctggccgacg gcggcacggt ccagatgccg ctgggaaaaa
     8701 ccttcttctc gccgcgcttc gggatggtcg ccgaccgctt cggcgtgtcg tggatggtga
     8761 tcgtgctgcc gtgaggagaa ggtccggaat cggctgcggg cgctgcgact ttcagctttt
     8821 cagccgatac ccggtcctga agatccacgc caccacggcg aggcaggcga cgaagaacgc
     8881 gagcgtcatg gcgagactca cggccacgct cacgtcggag accccgtaga agctccagcg
     8941 gtagccgctg atcaggtaca cgatcgggtt gaacaggctg aacgtgcgcc acgcgggcgg
     9001 cagcatgtcg atcgaataga acgcgccgcc caggaaggta agcggtgtga cgaccagcat
     9061 ggggacgaag ttgagctgct cgaaggtatt cgcccagatc ccgaggatga agccgaaaag
     9121 ggagaaagtc gccgccacga gcaccaggaa cgcgaccatc cacaccggat gatcgatgcg
     9181 caccgcgatg aacaggtttg cggtcgcaag aatgaccgcg ccgagcagga tcgacttggt
     9241 ggcggccgcg cccacgtagg cgagcacgat ctcgaaggcc gacaccggcg cggaaagcag
     9301 ttcgtagatg gtgccggcga atttcggcag gtagatgccg aaggaagcgt tgttgaggct
     9361 ctcggtgaag atcgaaagca tgatcaggcc cggcacgatg aaggcgccgt aggggacgcc
     9421 atcgacctgc gtcatgcgcg gcccgatcgc ggcaccgaag acgacgaaat aaagcgaggt
     9481 gatgatgacc ggcgtgacga ggctctgcca cagcgttcgc cggaagcgcg ccagctcgaa
     9541 gcggtagatg gccaggacgg cgtggacgtt catgggaatt tccgccccgg aggggacccc
     9601 gcgcggtcgc tcaccaggct gacgaagatg tcctcgagcg agctctgccg cgtgttcagg
     9661 tcggcgaacc cgatccccag gtcgtcgagc cggcgcaaca gggcggggac cgcggtgcgc
     9721 tcctcgttgt cgaaggtgta ctcgagcacc gatccttcct cgatcaatgc aaggttccag
     9781 tcggcgaggg ccgccgggac gctcgccagc ggctggagca ggtgcagcga aagctgcttc
     9841 ttgccgagct tcttcatcag ccgggccttt tcctctacca cgatcagctc gcccttgctg
     9901 atgacgccga tgcggtcggc catctcctcg gcttcctcga tgtagtgcgt ggtgaggatg
     9961 atggtgaccc ccgcgtcgcg caggcgccgc accagcgccc acatgtcgcg gcgcagctcg
    10021 acgtcgacac ccgcggtggg ttcgtcgagg aacaggatct ccggctcgtg cgagagcgcc
    10081 ttggcgatga ggacgcggcg cttcatcccg ccggagagcg tcatgatctt gtcgttgcgc
    10141 ttctcccaca gcgacagttc ccgcagcacc ttctccaggt gcgcggggtc cggcgcgcgg
    10201 ccgaagagcc cgcggctgaa gcacatcgtc gcccacaggg tctcgaacat gtccgtgtgc
    10261 agttcctggg gcaccaggcc gatcttcgag cgcgcgaggc ggtagccgct gacgatgtcg
    10321 tgcccgtcca cgcgcacggt tcccgtactc ggcgtcacga tgccgcagac gatgctgatc
    10381 agcgtggtct tgccggcgcc gttggggccg aggagcgcga agatctctcc cctgcggatc
    10441 tccagatcca cgcccttcag cgcctggagg ccggaggcat agctcttggt gaggccggcg
    10501 atcgagatca ccggcgggcg tggcgcggca gtcatggcga aggcgattct aagacattgg
    10561 ggacggttct ctgtacccgg tacctgtacc gggtacatgt accgggtaca accgtcccaa
    10621 aagaaaagcc ccgccgaagc ggggctttcc ggtgcaacgg ggctgcgcag gacttacatg
    10681 tccatgtcgc ccatgccgcc catgccgccc atgccaccca tgccgccggg catgccgccc
    10741 ttgtcgtcct tgggcagctc ggcgaccatc gcgtcggtcg tgagcatgag agccgcgacg
    10801 gaggccgcgt tctgcagggc gcagcgcgtg accttggtcg ggtcgagcac gcccatcgac
    10861 accatgtcgc cgtactcgcc ggtctgggcg ttgtagccgt agttgccctt gccttccatg
    10921 accttgttca ccaccacgct cggctcgtcg ccggcgttgc gggcgatctc gcgaagcggc
    10981 tgctccagcg cgcgaagcac gatcttgatg ccggcttcct ggtcggcatt gtcgcccttc
    11041 accttggaaa ccgcttcctt ggcgcgaagg agcgcgacgc cgccgccggg gacgatgcct
    11101 tcctcgaccg cggcacgcgt ggcgtgcaga gcgtcttcca cgcgggcctt cttctccttc
    11161 atctcgactt cggtcgcggc acccaccttg atcagcgcca cgccgccggc gagcttcgcc
    11221 acgcgctcct ggagcttttc cttgtcgtag tcgctggtgg cttcctcgat ctgggagcgg
    11281 atgatggaca cgcgcgcctt gatctggtct tccttgccgg agccgtcgat gatcgtggtg
    11341 tcttccttcg cgatctcgat gcgcttggcc ttgccgaggt ccttgagctg gacgttctcg
    11401 agcttgaggc cgagttcctc ggcgatgacc gtgccgcccg tgaggatggc gatgtcttcc
    11461 agcatggcct tgcggcggtc gccgaagccc ggggccttga cggcgcaggt cttgaggatg
    11521 ccgcgcaggt tgttcaccac cagggtggcg agcgcctcgc catcgacgtc ttccgcgacg
    11581 atgagcagcg gccggccggc cttggccacc tgctcgagca ccggcagcag gtcgcggatg
    11641 ttggagatct tcttgtcgtg cagcaggacg aacgggtcct cgagcacggc gatctggcga
    11701 tccgggttgt tgatgaaata cggcgacagg tagccgcggt cgaactgcat gccttccacg
    11761 atgtcgagct cgttgtcgag gctcttgccg tcctcgaccg tgatcacgcc ctccttgccg
    11821 accttgtcca tcgcatcgga gatgatcttg ccgatgttct cgtcggcgtt ggcggagatg
    11881 gcgccgacct gggcgatctc cttggaggtc gtgcagggct tggagatctt cttcagctcc
    11941 tcgacggtgg cgatgaccgc cttgtcgatg ccgcgcttca ggtccatcgg gttcatgccc
    12001 gcggccacgt acttcatgcc ctcggtcgcg atcgactggg caagcaccgt ggccgtggtg
    12061 gtgccgtcac cggcgatgtc ggaggtcttg gaggcgactt ccttcaccat ctgcgcgccc
    12121 atgttctcga acttgtcctt gagctcgatt tccttcgcga ccgacacgcc gtccttggtg
    12181 acggtggggg agccgaagga gcgctccagc accacgttgc ggcccttggg accgagggtg
    12241 accttcaccg cgtcggcgag aatgttcacg cccgtcatca tgcgaacgcg cgcgtgttcc
    12301 ttgaacttga cgtctttggc tgccatggga attcctctcc aaatctgttg ggtgaacgac
    12361 tcaggccacg acggccatga tgtcttcctc gcgcatcacg agcagttcct cgccgtcgat
    12421 cttgacggtg gtgcccgagt atttgccgaa gagaatcttg tcgccgacct tcacgtcgac
    12481 gggggaaagc ttgccttcct cgttcttctt gccgggaccg acggcgagca cttcaccctg
    12541 gtcgggcttc tcggcaacgg tgtcggggat cacgatgccg gaagcggtct tgcgctcctc
    12601 ttccaggcgc ttgacgatca cgcggtcatg cagcggacga agtttcattt cttgactcct
    12661 cgatgggtcg ggatgattgg aattggcgtt cagggcagga atccctgaag cggacaaaaa
    12721 ccggtattag cactcaccag tgacgagtgc taataatagg gctgcgcacg aggaaattca
    12781 agggcgatcg aagccggaaa ggctcgaacg cgaaggaagt ggcggaaatt gcgtaccgga
    12841 gcggagacgc gcccggaatg gaaacaagct caatttcaat gacttgggct aatgccgccc
    12901 agctgctgcc accaagtggg cggcggcagc ttccgaccgg acgcacgggc gtccgatcgg
    12961 aagctcgaat ccggcgtact ggccttcaca tcaccagccg gtcgctcctg ccgaactggt
    13021 acacgacgcg gcgcgacgca ccgggcttgg tcggggcggc gaggaacggg cacggcggat
    13081 tgcggtaacc accctgctcg agcgtcatgt cctcggcaac gtccgcccag acgccgccgc
    13141 gaacgggccg gaacatccac tgcacgtagc ccgagcggcc ggccgcgtcg atgagctcgt
    13201 cgccatccgg cacctcggcc acgatgggcg gcacgccctc gatcgcgatg ggcaggttgc
    13261 cgaaggcgcc catgcactcc agggattcct gcgcgctcga accgggccgg atgaaaggcg
    13321 gcgcctcgcg gcgcgcgagg tcccacgagc ccagctccca tgccacgtcg agcaccgcgt
    13381 tctccagttc ctcgaagcgg tcccagcggt cgttcacctg cagcgccgag ctcggcaccg
    13441 gctggtacgc gatgacgttg gcgtccgtca cgttggcgag cctcgtctcg acgaagccgt
    13501 gccagtgaag cgtgaggacc accggcgtat tggcatatgg gacagccgcg acgctgacac
    13561 cgaccagcgg cagtcggttg gcgatgagtt gcgactcgat gcagtctagt agtttcatgg
    13621 ttggaacctc cttgtgtgcg caagtgaagt gctgcattgc cgttcacctg aagcgaagca
    13681 agttgcatgc gatccatgca ccctgcggca ataaaccacg catcgcgcgc gggtccttcc
    13741 cgaggggaaa tggacgactt cgataagtcg ctgtttttgt tccgcggagt gccgaggccg
    13801 cgctcgacgc cgcgagcgcg ccggggcgcc tgcgccgttc gcgctgcgac gctttgccac
    13861 ggcgtccttt cgtgccgctt ccatctggtg ggaatgcccc agtcagcggc gggaaattgg
    13921 cgggatatgc cccgattcat caccggaacc ccgcccgccg gccgtcacgt cgctcaaggc
    13981 gcgataaaat gcctgcgcgc acgctgcgaa cggatcggga tcacatgcct ttgctgggac
    14041 cgagtgaaaa gggcgaactg gagagattga tcctcgacag tctcacggcg cactggaacg
    14101 cgcacgggtc cggcgggctc ctcgcccccg gcacgcgcgt ggcactggac acgaagggtc
    14161 cctcgatcgt cacggccgac gcgcgcggat tcctcgcatc ggtggaaatc cgcacgcttt
    14221 tcaccgccct gtgccacctg tccgccgacg gtcccgggtc gatgccccgc gacagcctca
    14281 ccgcgctggc cacggaggcc gccaactccc ttgcggcaca ggtcaaccgc ctcgtgcgcg
    14341 actgacgccc tcgtggccaa ccgcgacatc gtcgaacgca gcctcgcggc ggtctggcac
    14401 ccgtgcacgc agatgaagca gca
//
LOCUS       DASUEV010000257        15691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_641790, whole genome shotgun sequence.
ACCESSION   DASUEV010000257 DASUEV010000000
VERSION     DASUEV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641790"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFV55_08260"
     CDS             complement(<1..400)
                     /locus_tag="VFV55_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX4944333.1"
                     /translation="MAWQRRHGRGDLPWQGTRDPYRIWVSEIMLQQTQVATVIPYFER
                     FVARFPELRALAAAPIDDVMRLWSGLGYYARARNLHRAAQAVIDLHGGHFPLSLDEIA
                     ALPGVGRSTAAAIVVFATGEPHAILDGNVKR"
     gene            complement(449..522)
                     /locus_tag="VFV55_08265"
     tRNA            complement(449..522)
                     /locus_tag="VFV55_08265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Gly,seq:ccc)
     gene            602..808
                     /gene="thiS"
                     /locus_tag="VFV55_08270"
     CDS             602..808
                     /gene="thiS"
                     /locus_tag="VFV55_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX4944334.1"
                     /translation="MELLLKVNGAERRFAPPLTVRELVGKLGFEGRRVAIERNGEIVP
                     RSRYAEVELADGDRLEVVVAVGGG"
     gene            808..1599
                     /locus_tag="VFV55_08275"
     CDS             808..1599
                     /locus_tag="VFV55_08275"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX4944335.1"
                     /translation="MDDRLVIAGKAYRSRLLVGTGKYRDFEETRLAIEASGAEIVTVA
                     IRRVNIGQNAGEPSLLEVLPPSRYTILPNTAGCYTAEEAVRTLRLARELLDGHTLVKL
                     EVLGDAETLFPNMPETLAAAKTLVAEGFDVMVYCSDDPILARQLEDLGCVAVMPLASL
                     IGSGMGILNPWNLQIILDRARVPVLVDAGIGTASDAAVAMELGCDGVLMNTAIAKAQD
                     PVRMGRAMKLAVEAGREAWLAGRMAKKPYRAAASSPAEGLIGKTA"
     gene            complement(1604..2197)
                     /locus_tag="VFV55_08280"
     CDS             complement(1604..2197)
                     /locus_tag="VFV55_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944336.1"
                     /translation="MPSLKCLLPMLALAIPPGVLAAGDYVGVLRPVANPGAIAIPVPG
                     FYGSPASTFGGVPGPDWTTQGSKLKLGYKYSKFFSVEAGYVDFGPRAPALFSGTAARG
                     RGFTVETVGAIPLWARTSFYGRFGAWRSDGGMALLGGGDANPRPGAGLRYGLGLKYDL
                     TRRVGFQAGMDHFSPLDQWGSREPDTDQVTLGVTVRF"
     gene            2277..2975
                     /gene="trmB"
                     /locus_tag="VFV55_08285"
     CDS             2277..2975
                     /gene="trmB"
                     /locus_tag="VFV55_08285"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX4944337.1"
                     /translation="MDATNRPIRSFVLRQGRFSPGQQRAYEMHMPRFGVFFSGENIDF
                     EAVFGRNAPRILEIGFGMGETTARIAAENPENDYLGIEVHSPGVGALLKRIDEQALTN
                     IRIIQHDAVEVVQAMLPPGSLSGVHVFFPDPWPKKRHHKRRLIQPPFAELLASRLAPG
                     GYLHVATDWQEYAEWILATLSAVASLENVHGGFADRPEARPETKFEQRGRRLGHDSWD
                     VILRKRTSDPGGRR"
     gene            2972..3664
                     /locus_tag="VFV55_08290"
     CDS             2972..3664
                     /locus_tag="VFV55_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944338.1"
                     /translation="MSASDCDPIPGNRRRFLARAASSAFAVGGLSGFMSRALAKGDIA
                     AIPGVNRLEGTVTVNGRPAIVGMPVTASDKVSTGPGSMTVLVIRDDAFLLRADTTLEF
                     AQSGGALSGMLIRAGRVLSVFGRKPVAIRAGNASIGIRGTGAYLEVEPGKVYFCLCYG
                     EADIDGPGLAAPKNVKTTHHEQPLVLSERAGTMAAVPGPFINHSDDELILLESLVGRE
                     PPFVGTYPTKRY"
     gene            3755..4558
                     /gene="folE2"
                     /locus_tag="VFV55_08295"
     CDS             3755..4558
                     /gene="folE2"
                     /locus_tag="VFV55_08295"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEX4944339.1"
                     /translation="MNQRSQLPIPDVQSTPDVRRLAIDKVGIKSIRHPMKILERAGDI
                     QHTVANFSMYVGLPHQFKGTHMSRFVEILNAHEREITVDSFRAMLRQMVKKLEAETGH
                     IEMSFPYFVLKEAPVSGVKSLLDYEVTFIGEIVEGREKFALKVLVPVTSLCPCSKKIA
                     DYGAHNQRSHVTVTARVHSFVWIEEVIDLIEKQASSELYGLLKRPDEKYVTELAYDNP
                     KFVEDMVRDIAAKLNADTRVEAYVVESENFESIHNHSAYAMIRKNWPAC"
     gene            complement(4577..5197)
                     /locus_tag="VFV55_08300"
     CDS             complement(4577..5197)
                     /locus_tag="VFV55_08300"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX4944340.1"
                     /translation="MGVALAMALPAAATEVNVIGLFPGKAVVVIGRGAPRTLSAGQRT
                     PEGVLLIAADSRSATLEIDGKREVLELGQHVESAALTGALQSVTLAEDGNGHFTAEGQ
                     VNGGRVRFLVDTGATLVTLPAAEAQRLGIDYRRGRQTVSQTANGNVVVYRVRLDSVAL
                     GPMTITAVDAVVHESPGLDIALLGMSFLNRTEMRREGANLTLTKRY"
     gene            complement(5332..5817)
                     /locus_tag="VFV55_08305"
     CDS             complement(5332..5817)
                     /locus_tag="VFV55_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX4944341.1"
                     /translation="MPSFDIACEANLVEVKNAVEQANKEIANRFDFKGSDARVEQKEH
                     ELTLFADDDFKLGQVRDVLHAKFAKRGVDARLLTLDKPEKIGGDKVKQKVTVKNGVET
                     ELAKKIVKMLKDAKLKVTAAIQGDVVRVSGAKRDTLQEAIALVRSGVTEVPLSYQNFR
                     D"
     gene            complement(5864..6778)
                     /gene="argF"
                     /locus_tag="VFV55_08310"
     CDS             complement(5864..6778)
                     /gene="argF"
                     /locus_tag="VFV55_08310"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX4944342.1"
                     /translation="MRHFLQFRDFSAEEVGHLLKRTRVIKERFKNYVAYQPLSDRMLA
                     MVFEKSSTRTRVSFEAGMFQLGGASIVLNMGDTQLGRGEPIEDVARVVSRMVDIVMIR
                     TFEQSIIERFAAHSRVPVINGLTDEYHPCQVLADIYTFVEHRGAIAGKTVAWIGDSNN
                     MCNTWLQAAILLGFKLNVASPAGYELEPARAAGVGRDHLELFADPAAAARGADLVTTD
                     VWTSMGWEAETGARKDAFAAFTVDSRMMAEARKDALFMHCLPAHRGEEVSAEVLDGPQ
                     SVVWDEAENRLHAQKALMEYLLLGRVEN"
     gene            complement(6784..7980)
                     /locus_tag="VFV55_08315"
     CDS             complement(6784..7980)
                     /locus_tag="VFV55_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX4944343.1"
                     /translation="MSAAHLMKTYSPQPVAFARGEGAWLWDTQGKQYLDALAGIAVNG
                     LGHAHPLLTKAIAVQAAKLIHTSNLFHVVEQEMLAEKVCGLAGMDNAFFCNSGSEANE
                     CAIKLARLHGHQRGVENPSIVVMEKAWHGRTLATLSATGSRKAQAGFEPLMGGFLRVP
                     YNDLGAVERLREHPSVVAILMEVLQGEGGIHVADIEYVRKLREICDRRQWLLMIDEVQ
                     SGIGRTGKWFAHQWAGIVPDVMSLAKGLGSGVPIGACLARGVAAQVFKPGNHGTTFGG
                     GPLVSVAGLTTLEVIEKDGLLANATRQGEAIMQGLAREFSGVAGVKEIRGKGLMIGIE
                     LDRPCGDLVGRALQAGLVINVTADRVLRLLPPLIYGDAESRELVARLAPLVKAFLKEA
                     QPAVAG"
     gene            complement(8148..8420)
                     /gene="rpsT"
                     /locus_tag="VFV55_08320"
     CDS             complement(8148..8420)
                     /gene="rpsT"
                     /locus_tag="VFV55_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX4944344.1"
                     /translation="MANSKQAAKRAKQAGKQRTANMGLRTTLRTAIKKVKTAIAAGDV
                     KAAQAAFDANVSTIDRIADKKIIHKNKAARHKSRLAQQVKALAGGA"
     gene            8550..10082
                     /gene="murJ"
                     /locus_tag="VFV55_08325"
     CDS             8550..10082
                     /gene="murJ"
                     /locus_tag="VFV55_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX4944345.1"
                     /translation="MNLLKSAASVSAMTLVSRITGFVRDTLMAIAFGAGFSMDAFVVA
                     FRLPNLLRRLFAEGAFSQAFVPILGEYKSRRGEEATRELSARVLGSLSVALLVATIVG
                     VIAAPLLVYASAAGFAKDADKFALTVTMLRICFPYIFFISLTSFAAGILNTWGHFKVP
                     AFTPVLLNLSFIGFALFAAPYFDRPILALAWAVFAGGILQLAFQVPFLLRIRMLPRPR
                     WDPRDEGVVRVLKLMAPAALGVSVAQISLLINTHIASWLGDGPVSWLYFADRLMEFPS
                     ALLGVALGTVLLPSLVKHHNSNDPVAYSRLLDWGLRLTLLLALPAAVGLALLAVPLVA
                     TLFWHGEFTRHDVLMTRSALIAYAAGLAGIILVKILAPGFYARQNIRTPVKVAIATLA
                     VTQLANLVLVPWLRHAGLAAAISVGACFNAAWLWALMRRSGAYQPAPEWGAFLLKLAV
                     ALYLMGGVIWYGMGSEQSWFAITTGMRALKLAAVIAGAMATYFATLWALGLRLRQFSR
                     HE"
     gene            10079..11011
                     /locus_tag="VFV55_08330"
     CDS             10079..11011
                     /locus_tag="VFV55_08330"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX4944346.1"
                     /translation="MKLSVIRDPKPGAPDAVIAIGSFDGVHRGHRALLARVVAEAAEL
                     GAVPAVLTFEPLPRELLFPDRAPPRLSTLSAKLHAFADAGIGIAYVCRFTREFAALSP
                     EEFALRLKRSHGARRVLVGKDFRFGAKRAGDVGLLVAEGARLGYEVETLATVAEGEDR
                     VSSTRVREALAAGDLGLASRLLGQPYAICGRVVHGAKLGRSLGFPTVNIALPPGRPVM
                     TGVFAVRCVGAATRGLEGVASLGYKPVVASNGPATLEAYLFDFSGDLYGRRLSIEFLK
                     KLRDEAKYASIDELVAQIRLDCDAARAYFRETGT"
     gene            11011..13821
                     /gene="ileS"
                     /locus_tag="VFV55_08335"
     CDS             11011..13821
                     /gene="ileS"
                     /locus_tag="VFV55_08335"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX4944347.1"
                     /translation="MANDAKTDYKHTLNLPDTAFPMRGDLARREPEWVKAWQEKGLYR
                     RIREACRGRPLFVLHDGPPYANGDIHIGHAVNKILKDIVVKSKTMAGFDAPYTPGWDC
                     HGLPIEHMIEKKHGRHLEGNKARELCRAYAAEQIERQKADFRRLGVLGDWEHPYKTMD
                     FATEADEIRALARIWKKGLLYRGLKPVNWCIDCGSALAEAEVEYEDKTSAAIDVAFRA
                     ADTGAVARAFGVAQAPERVLAVIWTTTPWTLPGNQAIAAHPGHEYELVATERGALVLA
                     AELREACLKRYGLEATGILGHAAGSALAGLAFRHPFEPRDVPIVLGEHVTLEAGTGLV
                     HTAPAHGVEDFDTGVKYGLPLDQPVDDRGRFKASVPHFAGLGVREAEKPILELLASNG
                     ALLRNEPFRHSYPHCWRHKTPIIFRATTQWFIGMDQPSPAGRDNPGDNGRTLREIATQ
                     AVASTTFYPSWGRSRMEAMIANRPDWTLSRQRNWGVPLPFFLDRETEELHPETERLLE
                     EAAARVAKGGIEAWFAATCEDFGVDPAKYRKIADTVDVWFDSGTTHFSVLRGLAGAKW
                     PADMYLEGSDQHRGWFQSSLLTSCAMDGRAPYDSLLTHGFVVDGQGRKMSKSLGNVIA
                     PQKVSDTLGAEIIRMWVSSADYSGELYISDEILKRVVESYRRIRNTLRFLLANTADFD
                     RTKDLLPPESWPEIDRYALAMTREMAAAATADYARFEFHLVAQRIQTFCSEDLGGFYL
                     DILKDRLYTCGRDSAARRAAQSAVHHITQILLRLMAPILSFTAEEAWAVLHPGRDDSL
                     FFHTWNAVLPEQANEAALLVKWKRLREIRALVQKQLEELRQSGGIGSSLQAEVAIAAP
                     EGDRALLESLGSDLRFVLITSAATVEAGAALAVRVSPSTHAKCERCWHYVSDVGSDPR
                     HPGICGRCVSNIEGPGEERRHA"
     gene            13814..14326
                     /gene="lspA"
                     /locus_tag="VFV55_08340"
     CDS             13814..14326
                     /gene="lspA"
                     /locus_tag="VFV55_08340"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX4944348.1"
                     /translation="MPERGKAPWQRWLALSAAVTALDLATKAWISTAFTAGDVHVITP
                     FLNLVLWHNTGAAFSFLAGAGGWQRWFFIGVTLAISAVLLVMLRRSGGNRLLACALSL
                     VLGGAAGNLWDRITLGHVVDFIQLHAAGYYWPAFNVADSAITVGVALILWDGILESRA
                     SRGAGKEMTP"
     gene            14323..15276
                     /gene="ispH"
                     /locus_tag="VFV55_08345"
                     /gene_synonym="lytB"
     CDS             14323..15276
                     /gene="ispH"
                     /locus_tag="VFV55_08345"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX4944349.1"
                     /translation="MTDPIEIVLAKPRGFCAGVDRAIAIVERALEVHGAPIYVRNEIV
                     HNKFVVGDLRAKGAIFVEELAEVPAGGTVIFSAHGVSQAVRREAVERGLRVFDATCPL
                     VTKVHAEVARMREHGREVVMIGHEGHPEAEGTMGQSDAGMYLVETEEDVARLGVKDPG
                     NLAYVTQTTLSMDDAARIVAALRARFPAIVGPKKDDICYATQNRQDAVKFMAPRCDVV
                     IVVGSFNSSNSNRLREVAENLGRRAYLVDRAEDISPEWIAGARTIGVTAGASAPEVLV
                     RDVIAALRRLGATRVRELDGITENVTFPLPRSIAKADSGAA"
     gene            complement(15318..>15691)
                     /locus_tag="VFV55_08350"
     CDS             complement(15318..>15691)
                     /locus_tag="VFV55_08350"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEX4944350.1"
                     /translation="LTSYSTNLVASIQVARSEAIKRNTVVTLCASTDGAACADDWGNG
                     WIVLSGASVLQREQALPSGWRVTEKDGIGSLTFQPTGVGVTQAEFTFCRATPAGSQER
                     VLTVSATGTASVRKTTTGTCS"
BASE COUNT         2516 a         5402 c         5160 g         2613 t
ORIGIN      
        1 cgcgcttcac gttcccgtcg agtatcgcgt gcggctcacc cgtggcaaag accacgatcg
       61 ccgcggcggt cgaacgcccg acccccggca gcgccgcgat ctcgtcgagc gaaagcggaa
      121 aatgcccccc atgcaggtcg atgaccgcct gcgcggcccg gtgcaggttg cgggcccggg
      181 cgtagtagcc cagcccgctc cacaggcgca tcacgtcgtc gatcggtgcg gccgcgagcg
      241 cccgcaattc ggggaaccgc gcgacgaagc gctcgaagta cgggattacg gtcgccacct
      301 gcgtctgctg cagcatgatc tccgagaccc agatccggta cgggtcgcgc gtgccttgcc
      361 aggggagatc gccgcggccg tggcgtcgct gccaagccac gacacgagag gcgaacgaag
      421 gggagcggga gggcattgcg gagcggggtg gagcgggtga agggaatcga accctcgtcg
      481 taagcttggg aagcttctgc tctgccattg agctacaccc gcagcgaggc gccattttac
      541 ccgaggcgga tgtgatctgg ctcaaggcgc gacggggtgc atcccgggat agaatcgacc
      601 catggaactg ctgctgaaag tcaacggcgc cgaaaggcgc ttcgcccctc cgctcaccgt
      661 ccgggaactc gtcgggaagc tgggcttcga gggccggcgt gtcgcgatcg agcgcaatgg
      721 cgagatcgtc ccgcgcagcc gctatgccga ggtcgagctt gccgatggcg atcgcctcga
      781 ggtggtcgtc gcggtgggag gcggatgatg gacgatcgac tcgtcatcgc tggaaaggcc
      841 tatcgctcgc gcctcctggt gggcacgggc aagtaccgtg acttcgagga gacgcgcctc
      901 gccatcgaag cgagcggcgc ggaaatcgtg accgtggcca tccgtcgcgt gaacataggc
      961 cagaacgcgg gcgagccgag cctgctcgag gtgcttccgc cgtcgcgcta caccatcctg
     1021 cccaacaccg ccggctgcta cacggccgag gaagccgtgc gcacgctgcg acttgcccgc
     1081 gagctgctcg acgggcacac gctggtgaag ctcgaggttc tgggcgatgc cgagacgctc
     1141 ttcccgaaca tgcccgagac gcttgccgcg gcgaagacgc tcgtggccga aggtttcgac
     1201 gtgatggtct attgcagcga cgacccgatc ctcgcgcgcc agctggagga cctgggctgc
     1261 gtcgcggtga tgccgctcgc ctcgcttatc ggttcgggca tgggcatcct caacccctgg
     1321 aacctgcaga tcatcctcga ccgtgcccgg gttcccgtcc tggtcgatgc cggtatcggc
     1381 accgcgtcgg acgcagcagt cgccatggaa ctgggttgcg acggcgtcct catgaacacc
     1441 gccatcgcca aggcgcagga tcccgtgcgc atgggccggg cgatgaaact ggccgtcgag
     1501 gccgggcgcg aggcttggct ggctggccgc atggcgaaaa agccctaccg ggcggccgcc
     1561 agttcgcctg ccgagggtct gatcgggaaa acggcctgac ggcctagaag cgcacggtga
     1621 cgccgagcgt gacctggtcg gtgtcgggct cgcgcgagcc ccactggtcg agcggcgaaa
     1681 agtgatccat cccggcctgg aacccgaccc gccgggtcag gtcgtacttg aggcccagcc
     1741 cgtagcgaag cccggcgccc ggtcgcgggt tcgcgtctcc cccgcccagc aacgccatcc
     1801 cgccatcgga tcgccaggcg ccgaaccggc catagaatga cgtacgcgcc cagaggggaa
     1861 tggcgccgac ggtttcgaca gtgaatcccc tgccccgcgc agcggtgccg gaaaaaagtg
     1921 cgggcgccct gggaccgaaa tcgacgtagc ccgcctccac cgagaagaat ttcgagtact
     1981 tgtaaccgag cttcagcttc gacccctgcg tcgtccaatc cgggccaggc acgccaccga
     2041 aagtactggc cggcgacccg tagaacccgg gcaccgggat tgcaatggcg cctggattcg
     2101 ccactgggcg cagcacgccc acgtaatcac ccgctgccag gacgccgggc gggatggcga
     2161 gggcaagcat cggcagcagg catttgagcg agggcatacg aagatagtgg gggttgtctc
     2221 ttttcggcgt caatgaaaac gccgtccacg ggcggctcgc agcgtaaaat tgaggcatgg
     2281 acgcaacaaa ccggcccatc cgcagctttg tccttcgtca ggggcgcttt tcgccagggc
     2341 agcagcgtgc ctacgaaatg cacatgccgc ggtttggagt ctttttttcg ggcgaaaata
     2401 tcgatttcga ggccgttttc ggcaggaatg ccccgagaat cctcgaaatc ggcttcggaa
     2461 tgggggagac gacggccagg attgcggccg agaacccgga aaacgactat ctggggatcg
     2521 aagttcattc cccgggggtt ggtgcgctgc tgaagcggat agatgagcag gcactcacaa
     2581 acatcaggat catccagcac gatgccgtcg aggtcgtgca ggcgatgctg ccaccgggat
     2641 cgctatccgg cgtccacgtg ttcttccccg acccgtggcc caagaagcgc caccacaaac
     2701 gccgcctcat ccaaccgccg ttcgcggagc tccttgcaag ccgtcttgcc cccgggggct
     2761 acctgcatgt ggcaaccgac tggcaggaat atgccgagtg gatcctggcg accctttcgg
     2821 ccgtggcatc cctcgagaat gtccacggcg gctttgccga ccgccccgag gcccgtcccg
     2881 agaccaagtt cgagcagcgc ggacgccgcc ttggccacga ctcctgggac gtgatcctcc
     2941 gcaaacgaac gtccgacccg ggaggccgtc gatgagtgcc agcgattgcg atccaatccc
     3001 ggggaatcgc cgccgcttcc tggcccgcgc cgcctccagc gcctttgccg tgggcggcct
     3061 ttccggcttc atgagccggg cgctggcgaa gggtgatatc gcggcgatcc cgggcgtcaa
     3121 ccggctcgag gggaccgtga cggtaaatgg ccgccctgcc atcgtgggca tgcccgtcac
     3181 cgcaagcgac aaggtatcca cgggcccggg gagcatgacc gtgctggtga tccgggacga
     3241 tgcgttcctc ctgcgcgcgg acaccaccct cgaattcgcg caatccggcg gcgccctgag
     3301 cggcatgctc atccgggccg gtcgcgtgct ctccgtcttc ggccggaagc cggtcgcgat
     3361 cagggccggc aacgcgtcca tcggaatccg cggcaccggt gcgtacctcg aagtggagcc
     3421 cggcaaggtc tatttctgcc tctgctacgg cgaggcggac atcgacgggc ccggcctggc
     3481 ggccccgaag aacgtgaaga ccacgcacca cgagcaaccc ctcgttctca gcgagcgcgc
     3541 gggcaccatg gccgctgtcc cagggccttt catcaaccac tccgacgacg aattgatcct
     3601 ccttgaatcg cttgtcggac gggaaccgcc cttcgtgggc acctatccca cgaagagata
     3661 ttgagcgccg cggtgagtcg aaggcgccat agttgagtgg tttactcaac tatcaaggca
     3721 ccctatactc acgactgccc gacgaggaaa cccagtgaac cagcgaagcc agctccccat
     3781 cccggacgtc cagtccaccc ccgacgtccg ccggcttgcc atcgacaagg tggggatcaa
     3841 gtccatccgt cacccgatga agatcctcga gcgcgcgggc gacatccagc acacggtcgc
     3901 gaacttcagc atgtacgtgg ggcttccgca ccagttcaag ggaacgcaca tgtcgcggtt
     3961 cgtggagatc ctcaacgccc acgagcgcga gatcaccgtc gactcgttcc gcgccatgct
     4021 gcgccagatg gtgaagaagc tcgaggccga gacgggccac atcgagatga gcttcccgta
     4081 cttcgtcctc aaggaggcgc ccgtgtcggg cgtgaagagc ctcctcgact acgaggtcac
     4141 cttcatcggc gagatcgtcg agggccgcga gaaattcgcg ctcaaggtcc tggtgccggt
     4201 gacgagcctg tgcccctgct cgaagaagat cgcggattac ggcgcccaca accagcgctc
     4261 gcacgtgacc gtcaccgcac gcgtgcacag cttcgtctgg atcgaggaag tcatcgacct
     4321 gatcgagaag caggcctcga gcgagctcta cgggctgctc aagcgccccg acgagaagta
     4381 cgtgaccgag ctcgcctacg acaaccccaa gttcgtcgag gacatggtcc gcgacatcgc
     4441 cgcgaagctc aacgccgaca cgcgcgtcga ggcctacgtg gtggaatcgg agaatttcga
     4501 gtccatccac aaccactcgg cctacgcgat gatccgcaag aactggcccg cctgctgaca
     4561 gcgcgggccc ggtacctcag tagcgtttcg tgagggtgag gttcgcgccc tcccggcgca
     4621 tctccgtgcg gttcaggaag ctcattccca gcagcgcgat gtcgagcccc ggggactcgt
     4681 gcaccaccgc gtccacggcc gtgatggtca tcgggcccag ggccacggaa tcgaggcgca
     4741 cccggtacac gacgacattc ccgttggcgg tctgcgatac cgtctgccgt ccgcgccggt
     4801 aatcgatgcc caggcgctgc gcttccgcag ccggcagggt cacgagcgtg gcgccggtat
     4861 cgacgaggaa ccgcacgcgt ccgccgttca cctgtccctc ggccgtgaag tgcccgttgc
     4921 cgtcctcggc aagcgtgacg ctttgcaggg ccccggtgag tgctgccgat tccacgtgct
     4981 gccccagttc gaggacctcg cgcttgccgt cgatctcgag cgtggccgag cgcgagtccg
     5041 cggcgatgag cagcacccct tcgggcgtgc gctgtccggc ggacagcgtg cgcggcgcgc
     5101 cgcgcccgat caccacgacg gccttcccgg gaaagaggcc tatcacgttc acctcggtcg
     5161 ccgcagcggg cagcgccatg gcgagcgcga cccccatgga atgccgaaac cgtcggaggg
     5221 tgcccatttt tggggggaaa ggggtcaggt tactttagga aaaaggggtc aggttacttt
     5281 actgtcgacg gatttctacg gggctgtaaa gtaacctgac cccttttccc cctagtcgcg
     5341 gaagttctgg tacgagagcg ggacttcggt cacgcccgag cgcaccagcg cgatcgcctc
     5401 ctgaagcgtg tcgcgcttgg cgccggagac gcgcacgacg tcgccctgga tcgcggccgt
     5461 gaccttgagc ttcgcgtcct tgagcatctt cacgatcttc ttcgcaagct cggtctccac
     5521 gccgttcttg acggtgacct tctgcttcac cttgtcgccg ccgatcttct ccggcttgtc
     5581 cagcgtgagc aggcgcgcgt ccacgccgcg cttggcgaac ttggcgtgaa gcacgtcacg
     5641 cacctgccca agcttgaagt cgtcgtcggc gaacagggtg agctcgtgct ccttctgctc
     5701 gacgcgcgcg tccgacccct tgaagtcgaa gcggttggcg atctccttgt tggcctgctc
     5761 gacggcgttc ttcacctcga ccaggttggc ttcgcacgcg atgtcgaatg agggcatggg
     5821 ttctccggtg gcgggttgct gccgctcttt gcgcggcgtg ggttcagttc tcgacgcgcc
     5881 cgagcagcag gtactccatg agcgccttct gcgcatgcag gcggttttcc gcctcgtccc
     5941 agaccacgct ctgcggcccg tcgagaacct cggcggaaac ttcctcgccg cggtgggcgg
     6001 gcaggcagtg catgaacagg gcgtccttgc gggcctcggc catcatccgg gagtccacgg
     6061 tgaacgcggc gaacgcatcc ttcctcgcgc ccgtctccgc ctcccagccc atgctcgtcc
     6121 acacgtcggt ggtcacgagg tcggcgccgc gcgcagcggc ggccggatcg gcgaagagct
     6181 cgaggtggtc ccgcccaacg ccggctgcgc gtgcgggctc cagttcgtag cctgcggggc
     6241 tggctacgtt cagcttgaag ccgaggagga tcgcggcctg aagccaggtg ttgcacatgt
     6301 tgttgctgtc gccgatccag gccacggtct tcccggcgat ggccccgcga tgctccacga
     6361 acgtgtagat gtcggcgagc acctggcaag ggtgatattc gtcggtgagg ccgttgatca
     6421 cgggaacgcg ggaatgcgcc gcgaagcgct cgatgatcga ctgctcgaag gtgcggatca
     6481 tgacgatgtc gaccatgcgc gatacgacgc gcgccacgtc ctcgatgggc tcgccccggc
     6541 ccagctgcgt gtcgcccatg ttgagcacga tggaggcgcc gcccagctgg aacatgcccg
     6601 cctcgaacga cacgcgcgtg cgcgtcgagc tcttctcgaa caccatcgcg agcatccggt
     6661 cggaaagcgg ctggtaggca acgtagttct tgaaccgttc cttgatcacg cgcgtgcgct
     6721 tgaggaggtg cccgacctcc tccgccgaga agtcgcggaa ctgcaggaag tgcctcatcg
     6781 cggctatccg gcgacggcgg gctgcgcctc tttcaggaac gccttcacca gcggcgccag
     6841 gcgcgcgacg agctcgcgcg attcggcgtc gccatagatg agcggcggca ggagccgcag
     6901 caccctgtcc gcggtcacgt ttatcaccag ccccgcctgc agcgcgcggc ccacgaggtc
     6961 gccgcacggc cggtcgagct cgatgccgat catgagtccc ttgccgcgga tctccttcac
     7021 gcccgcaacg cccgagaact cgcgggccag accctgcatg atggcctcgc cctggcgggt
     7081 ggcattggcc agcaatccgt ccttctcgat gacttccagc gtcgtgaggc cggcgacgga
     7141 taccagcggg ccacccccga aagtggtgcc atggtttccc ggcttgaaca cctgcgccgc
     7201 gacaccgcgc gccaggcacg cgccgatggg cacgccggaa cccaggccct tggcaagcga
     7261 catcacgtcg gggacgatgc ctgcccactg gtgggcgaac cacttgccgg tgcgcccgat
     7321 gccgctttgc acttcgtcga tcatcagcag ccactggcgg cggtcgcaga tctcgcgcag
     7381 tttcctcacg tactcgatgt ccgcgacgtg gatgccgccc tccccctgca gcacctccat
     7441 caggatggcg acgacggaag ggtgctccct cagccgctcg accgcgccca ggtcgttgta
     7501 gggaacgcgc aggaacccgc ccatcagcgg ctcgaacccg gcctgcgcct tgcgcgagcc
     7561 ggtggccgaa agggtggcca gcgtgcggcc gtgccaggcc ttttccatca ccacgatgga
     7621 cgggttctcg acgccgcgct ggtggccatg caggcgagcg agcttgatgg cgcactcgtt
     7681 ggcctccgac ccggaattgc agaagaatgc gttgtccatg ccggcgagcc cgcagacctt
     7741 ctcggcgagc atttcctgct cgaccacgtg gaagaggttc gacgtgtgga tgagcttcgc
     7801 ggcctgcacg gcgatcgcct tcgtgagcaa agggtgggca tgcccgaggc cgttcaccgc
     7861 gatgcccgcc agggcgtcca ggtactgctt gccctgcgtg tcccacagcc aggcaccctc
     7921 gccgcgcgcg aacgccacgg gctggggcga ataggtcttc atgagatggg cggcagacat
     7981 gagcgcctcg cagaaacgat cacggcggcc ttacgccgcc gtggatccag gaaggtcgat
     8041 tatcggcaaa ttcacggggt gtcgcaaccg caggcgggcg cctgcgacac gacgagccgg
     8101 cggttgacga tcctcgctcc gggggcagga cagcccccgc gaagaaccta tgcgccgccg
     8161 gccaaagcct tgacctgttg cgcgaggcgg ctcttgtggc gggcggcctt gttcttgtgg
     8221 atgatcttct tgtccgcgat ccggtcgatg gtggatacgt tcgcgtcgaa cgcggcctgg
     8281 gcggccttga cgtcaccagc ggcgatcgcg gtcttcacct tcttgatggc ggtgcgaagg
     8341 gtggtgcgaa ggcccatgtt ggcggtgcgc tgcttgcccg cctgcttggc gcgtttcgcg
     8401 gcctgcttgg agttggccat attgatcctg atgtcggggg tttcgaaaac gaagcctgat
     8461 attatacagc gcttgtcctg tcaaaacaaa gccctagctt cccctccgct tttccagccc
     8521 gctgctgccc aagccgcgag cccgcgccca tgaacctcct caagtcggcc gcctcggtca
     8581 gcgcgatgac tctcgtgtcg cgcatcaccg ggttcgtgcg cgataccctc atggccatcg
     8641 ccttcggggc cggcttctcg atggacgcgt tcgtggtcgc cttccgcctg ccgaacctgc
     8701 tgcggcgcct gttcgccgag ggcgcgttct cgcaggcgtt cgtgcccatc ctgggcgaat
     8761 acaagtcccg gcgcggcgag gaggctacgc gggaactctc ggcgcgcgtg ctgggctcac
     8821 tgtccgtggc gctcctcgtc gcgacgatcg tgggcgtgat cgccgccccg ctcctcgtct
     8881 acgcgagcgc cgcgggcttc gccaaggacg cggacaagtt cgccctgacg gtgacgatgc
     8941 tgcggatctg cttcccgtac atcttcttca tctccctcac cagcttcgcg gcgggcatcc
     9001 tgaacacgtg ggggcacttc aaggttcccg ccttcacgcc ggtgctgctc aacctttcct
     9061 tcatcggctt cgccctgttc gccgccccgt acttcgatcg cccgatcctc gcgctggcct
     9121 gggccgtgtt tgcggggggc atcctgcagc tcgccttcca ggtgccgttt ctcctgcgca
     9181 tccgcatgct gccgcggccg aggtgggacc ccagggacga gggcgtcgtc cgcgtgctca
     9241 agctcatggc cccggctgcg ctgggcgttt cggtggcgca gataagcctc ctcatcaata
     9301 cgcacatcgc ctcgtggctg ggcgacgggc cggtgtcgtg gctgtacttc gccgaccggc
     9361 tcatggaatt cccctcggcc ctgctcggcg tggcgctcgg caccgtgctg ctgccctcgc
     9421 tcgtgaaaca ccacaacagc aacgacccgg tggcctattc gcgcctcctc gactggggct
     9481 tgcgactcac gctgctgctc gcgctgcccg ccgccgtcgg gctggcactc cttgccgtcc
     9541 cgctggtggc caccttgttc tggcacggcg agttcacgcg tcacgacgtg ctgatgaccc
     9601 gctccgcgct catcgcgtac gccgcggggc ttgccggcat catcctggtg aagatcctgg
     9661 ccccggggtt ctacgcccgc cagaacatcc gcacgccggt gaaggtggcg atcgccacgc
     9721 tcgccgtcac gcagcttgcc aacctcgtgc tcgtgccctg gctgcgccat gcggggctgg
     9781 cggcggccat ctcggtgggc gcctgcttca atgccgcatg gctgtgggcg ctgatgcgcc
     9841 ggtcgggggc gtaccagccc gcacccgagt ggggagcgtt cctgctcaag ctggccgtcg
     9901 ccctctactt gatgggcggc gtgatctggt acggcatggg ttccgagcaa tcctggttcg
     9961 ccatcaccac cggcatgcgc gccctcaagc tcgcggcggt gatcgccggc gcgatggcga
    10021 cgtacttcgc caccttgtgg gcgctgggcc tgcgcctgcg ccaattctcg aggcacgaat
    10081 gaagctctcc gtcatccgcg acccgaagcc cggcgctccc gatgcggtca tcgccatcgg
    10141 cagcttcgac ggcgtgcatc gcggccatcg cgcgctgctc gcccgcgtgg tggccgaggc
    10201 cgcggaactg ggcgccgttc ccgccgtgct cacgttcgag ccgctgcccc gcgaactgct
    10261 gtttccggat cgcgcgccgc cccgcctgtc cacgctctcc gccaagctgc acgctttcgc
    10321 cgatgcgggc atcgggatcg cctacgtctg ccggttcacc cgcgagttcg ccgcactttc
    10381 gcccgaggag ttcgcccttc gcctgaagcg atcgcacggc gcgcgccgcg tgctggtcgg
    10441 aaaggatttc cgcttcggcg caaaacgcgc gggcgacgtg ggattgctcg tggccgaggg
    10501 cgcgcggctg ggctacgagg tggaaacgct ggccacggtg gccgagggcg aagaccgggt
    10561 gtcgagcaca cgcgtgcgcg aggccctggc ggcgggcgac ctcggcctcg cgtcgcggct
    10621 cctcgggcag ccctatgcca tctgcggccg cgtggtgcat ggcgcgaagc tcggacgcag
    10681 cctcggtttc cccacggtca acatcgcgct gccgcccggg cgacccgtga tgaccggggt
    10741 ctttgcggta agatgcgtgg gtgcggcaac tcgcggactc gagggagtgg cgagcctggg
    10801 atacaagccg gtcgtggcat ccaacggccc ggcgactctc gaagcgtacc tcttcgattt
    10861 ctcgggcgac ctctacgggc gtcgcctctc catcgagttc ctgaagaagc tgcgcgacga
    10921 ggcgaagtac gcctcgatcg acgagctggt ggcgcagatc cgcctggact gcgacgcagc
    10981 gcgggcctat ttccgcgaaa cggggacctg atggcaaacg atgcgaagac ggactacaag
    11041 cacaccctga acctgcccga cacggcgttc ccgatgcgcg gcgacctcgc caggcgggag
    11101 ccggagtggg tgaaggcgtg gcaggaaaaa ggcctgtacc gccgcatccg cgaggcttgc
    11161 cgtgggcgcc ccctgttcgt gctccacgac ggcccgccct acgccaacgg cgatatccat
    11221 atcggccacg cggtgaacaa gatcctcaag gacatcgtcg tgaagtcgaa gacgatggcc
    11281 ggcttcgacg cgccctacac gcccggctgg gactgccacg gcctgcccat cgagcacatg
    11341 atcgagaaga agcatggccg ccacctcgag ggcaacaagg cgcgcgagct ctgccgcgcc
    11401 tatgccgccg agcagatcga gaggcagaag gcggacttcc gccgcctggg cgttctcggc
    11461 gactgggaac acccctacaa gacgatggat ttcgcgacgg aagccgacga gatccgggcg
    11521 ctggcgcgca tctggaagaa gggccttctc taccgcggcc tcaagccggt caactggtgc
    11581 atcgactgcg gatcggcact cgccgaggcc gaggtcgagt acgaggacaa gacctccgcc
    11641 gccatcgacg tcgcgtttcg cgcggccgac acgggtgccg tcgcgcgtgc cttcggcgtg
    11701 gcgcaggcgc cggagcgtgt gctggcggtc atctggacca ccacgccgtg gacgctgccc
    11761 ggcaaccagg cgatagccgc gcacccgggc cacgagtacg agctcgtcgc cacggagcga
    11821 ggcgcgctcg tcctcgccgc ggaacttcgc gaagcctgcc tgaagcgcta tggcctggag
    11881 gccaccggaa tcctcggcca cgccgcgggc agcgcgctcg ccggccttgc tttccgccac
    11941 cccttcgagc cgcgcgacgt cccgatcgtc ctcggcgagc atgtgacgct cgaggcggga
    12001 accggcctgg tgcacaccgc gcccgcgcac ggcgtcgagg acttcgacac cggcgtgaag
    12061 tacggcctgc cgctcgacca gcccgtggac gaccgcggcc gcttcaaggc cagcgtgccg
    12121 catttcgccg ggctcggcgt gcgcgaggcg gaaaagccga tcctcgagct gctggcttcc
    12181 aacggcgctc tcctgcgcaa cgagccgttc cgccacagct acccgcattg ctggcggcac
    12241 aagacgccga tcatcttccg cgccaccacg cagtggttca tcgggatgga ccagccttcg
    12301 cccgcaggaa gggacaaccc cggggacaat ggccgcacgc tgcgcgaaat cgcgacgcag
    12361 gccgtcgcca gcaccacgtt ctatccgtcg tgggggcgct cgcgcatgga agcgatgatc
    12421 gccaaccgcc cggactggac gctttcgcgc cagcgcaact ggggcgttcc gctgccgttc
    12481 ttcctggacc gcgagaccga ggagctccac cccgaaacgg aacggctcct ggaggaggcc
    12541 gcggctcgcg tggccaaggg cgggatcgag gcctggttcg cggcgacctg cgaggatttc
    12601 ggcgtcgatc ccgcgaagta ccgcaagatc gccgacaccg tcgacgtctg gttcgactcc
    12661 ggcaccacgc acttctcggt gctgcgcggc ctcgcggggg caaagtggcc cgccgacatg
    12721 tatctcgaag gctcggacca gcaccgcggg tggttccagt cgagcctgct caccagttgc
    12781 gccatggacg ggcgcgcccc gtatgactcg ctcctcaccc acgggttcgt ggtggacggg
    12841 caggggcgca agatgtcgaa gtcgctcggc aacgtcatcg ccccgcagaa ggtctccgac
    12901 acgcttggcg cggaaatcat ccgcatgtgg gtctcgtcgg cggactactc cggcgagctc
    12961 tacatctccg acgagatcct caagcgggtg gtggaaagct accggcgcat ccgcaacacg
    13021 ctgcgcttcc tgctcgccaa caccgcggac ttcgaccgta cgaaggatct gctgcccccg
    13081 gagagctggc ccgagatcga ccgctacgcc ctggcaatga cgcgcgagat ggccgcggcc
    13141 gcaaccgcgg actatgcgcg cttcgaattt cacctcgtgg cgcagcgcat ccagaccttc
    13201 tgttccgagg acctcggcgg tttctacctc gacatcctga aggaccgcct ctacacctgc
    13261 ggccgcgact cggcggccag gcgcgcggcg cagtcggcgg tgcatcacat cacgcagatc
    13321 ctcctccggc tcatggcgcc gatcctgtcc ttcacggccg aagaggcctg ggccgtgctg
    13381 cacccggggc gcgacgacag cctcttcttc cacacctgga acgcggtact gccggaacag
    13441 gcgaacgagg cggcgttgct ggtgaaatgg aaacggctgc gcgagatccg cgcgctggtg
    13501 cagaagcagc tcgaggaatt gcgccagtcc ggcggcatcg gctcgtcgct gcaggccgag
    13561 gtcgcgatcg ccgcgcccga gggcgaccgc gcgcttctcg aaagcctcgg ctccgacctg
    13621 cgctttgtcc tgatcacctc cgccgccacc gtcgaggcag gcgcggcgct cgccgtgcgc
    13681 gtgtcgccca gcacgcacgc gaagtgcgaa cggtgctggc attacgtgag cgacgtcggc
    13741 tccgacccgc gccaccccgg catctgcgga cggtgcgtgt ccaacatcga aggtcccggc
    13801 gaggagcggc ggcatgcctg agcgcggcaa ggcgccctgg caacggtggc tcgctctgtc
    13861 ggcagcggtc actgccctcg accttgcgac caaggcctgg ataagcaccg cgttcacggc
    13921 cggggacgtg cacgtgatca cgcccttcct gaatctcgtg ctctggcaca acaccggcgc
    13981 ggcgttcagc tttctcgccg gcgccggcgg ctggcagcgc tggttcttca tcggggtgac
    14041 gctcgccatt tccgccgtgc tcctggtgat gctgcggcga agcgggggca accgcctgct
    14101 cgcctgcgcg ctttccctcg tcctcggggg cgcagccggg aacctgtggg accgcattac
    14161 cctcgggcac gtggtcgact tcatccagct ccacgcagcg ggatactatt ggcctgcctt
    14221 caacgtcgcc gattcggcaa tcacggtggg ggtggcgctc atcctgtggg acggcatcct
    14281 cgagtcgcgc gcctcgcgcg gtgccggcaa ggaaatgaca ccatgaccga tccgatcgaa
    14341 atcgtcctcg ccaagccgcg cgggttctgc gccggcgtcg accgcgccat cgccatcgtc
    14401 gaacgcgccc tcgaggtgca cggcgccccc atctacgtgc gcaacgagat cgtgcacaac
    14461 aagttcgtcg tgggggacct gcgcgcgaag ggcgccatct tcgtcgagga gctcgcggag
    14521 gttcccgccg ggggcacggt catcttctcg gcccacggcg tgtcgcaggc ggttcgccgg
    14581 gaagccgtgg aacgtggact gcgcgtcttc gacgccacct gcccgctcgt gaccaaggtg
    14641 cacgcggagg tcgcgcgcat gcgcgagcac ggccgcgagg tggtgatgat cggccacgag
    14701 ggccacccgg aggcggaggg caccatgggg cagtcggatg ccggcatgta cctcgtggag
    14761 accgaggagg acgtggcgcg cctcggcgtg aaggaccccg gcaacctcgc ctacgtgacc
    14821 cagacgacgc tttccatgga tgacgccgcg cgcatcgtcg ccgccctgcg tgcgcgcttc
    14881 cccgcgatcg tggggccaaa gaaggacgac atctgctacg ccacccagaa ccgccaggac
    14941 gccgtgaagt tcatggcgcc gcgctgcgac gtggtgatcg tcgtgggttc gttcaacagc
    15001 tccaactcga atcgccttcg cgaggtggcg gaaaacctcg gtcgccgggc ctacctggtc
    15061 gaccgcgccg aggacataag cccggagtgg atcgcggggg cgcggacgat cggcgtcacc
    15121 gccggcgcgt ccgcccccga agtgctggta cgcgacgtga tcgccgcgct gcgccgtctc
    15181 ggcgccaccc gcgtgcggga actcgatggc atcaccgaga acgtcacctt cccccttccc
    15241 cgatccatcg cgaaagcgga ctccggcgct gcttgagcgg gagcccacgc gccgtgaccg
    15301 ttgccgtacg agtccctcta cgagcaggta ccggtcgtcg tcttcctgac cgaagccgtg
    15361 cccgtggcgc tgaccgtcag cacgcgctcc tggctgcccg ccggcgtggc gcggcagaac
    15421 gtgaattcgg cctgcgtcac ccctacgccg gtcggctgaa aggtcaggct tccgatcccg
    15481 tccttctcgg tcaccctcca cccggaaggc agggcctgtt cgcgctgcag cacactggcg
    15541 ccgctcaaca cgatccagcc gtttccccag tcgtccgcgc aggcggcgcc atcggtggaa
    15601 gcgcaaagcg tgaccacggt attgcgcttg atggcctcgc tgcgggcaac ctggatgctg
    15661 gccacgagat tggtggaata ggacgtgagc t
//
LOCUS       DASUEV010000258         8042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1449062, whole genome shotgun sequence.
ACCESSION   DASUEV010000258 DASUEV010000000
VERSION     DASUEV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449062"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1669)
                     /locus_tag="VFV55_08355"
     CDS             complement(122..1669)
                     /locus_tag="VFV55_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4944351.1"
                     /translation="MYEQLGIALVNIAQFKYLVPLFLGTLAGSVGGALPGVTITMTII
                     VALPFTFGLDPLQGLAAMTGLYVGGSAGGLVTAVLLGIPGTPSAVATTFDGHPMAVIG
                     EPGRAIWLGIWASFFGGLIGGMFLIAATGPLAAFALEFGPWEYFSLFVLALSMVAGLV
                     EASLLKGLISGMIGLIVTVLGNDPMLGTPRLTLGIEFLEGGVPFLPVLIGVFAFAQIM
                     SEVERPGGSGSAARAIDAAANLKVSQLRVIGEILSRPFLLLWSSIIGVWIGVLPAIGG
                     SAANMMAYDQAKKFSKRPEQFGQGIPEGIIASESSNNANVGGSLVTIMAFGIPGDAVT
                     AVMLGALTIHGIQSGPLFIAQHPTIAYGIFAAYLLAHPIMVLIMGLGARWALRIVTVP
                     KSILFPVVLVLCVVGAYALGNSMSSVYVLLAFGVLGYLMVKTGIPLAPFILGVILGDQ
                     IETNLVRSIMTDANPWLFVTRPISGALLGLSVASVVFALWQHRRAERLRRAREAATEA
                     ATQAADF"
     gene            complement(1672..2145)
                     /locus_tag="VFV55_08360"
     CDS             complement(1672..2145)
                     /locus_tag="VFV55_08360"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4944352.1"
                     /translation="MGRDGFTGLVVLAASVALFWATLGLEQNPLVPIGPAFYPRIVLG
                     ITAALAALLVAADVLAHRRAGGAPPSPRPRLRYAMVVALFALFGAYVVALPYAGFRIA
                     TFAFVAAMQAALEPPRGMRRWALVALVAFVATAGTYYTFDAYLKVLLPRGSWTDF"
     gene            complement(2168..3112)
                     /locus_tag="VFV55_08365"
     CDS             complement(2168..3112)
                     /locus_tag="VFV55_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944353.1"
                     /translation="MTRFLALLATLAWVGIFSASAAYPDRPVKLIVPWAAGGDTDVVY
                     RAFQPFLQKHLGGTIVIANVGGASGTKGAKEAKDSPADGYTIFAVHDSIHSTYYTGVA
                     DVAWTDFQPICLVASTPSILTASPKSTWKDAKAMLADAKARPGQIIVGASLGSTSHFF
                     PAMVEKAAGIKFKYVPYEGTAPRMNALLGSHIDMGESNLTQKGKADAGQMRFLAIATD
                     KRNAEVPDVPTLKELGVDVTYAVNRGLIAPKATPADVMAKLQSACAAAAKEPGFAEAM
                     KSQGTLVNFLGPKEYTGFLQGNDKINHDLAKDLGLLKR"
     gene            3207..4019
                     /locus_tag="VFV55_08370"
     CDS             3207..4019
                     /locus_tag="VFV55_08370"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX4944354.1"
                     /translation="MRIGLLGGGVIARLFLDHVHGAEGDARVVAIAGRSLSSRSRALA
                     DERGVPFVVGAAALVATRPDVVVEAASHDAVREHGETLLGSGIAMIVLSGGALADDEL
                     RGRLERAAARGRALLYVPSGGIGGLDALKAACAAGVEEVSISVTKPPAAWKDIAYVER
                     LGIDLDRLAGPVTLFEGSAREGVPHFPANVNIAAVLALAGAGFDRTRLRVVADPALRF
                     NTHFIRVKGRTGTIDMRFESVPSPDNPKTAMLACYSALAAFRQFASPVRYGT"
     gene            4112..4804
                     /locus_tag="VFV55_08375"
     CDS             4112..4804
                     /locus_tag="VFV55_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4944355.1"
                     /translation="MGKYVIDIGPLPSLPVAGSDARFPVGRIYCVGRNYAEHAREMGH
                     DPDREPPFFFMKAANSIVENNATISYPVGTKDVHHEIEMVVAIGKGGKNIPVEKALDH
                     VWGYGVGLDMTRRDIQGEAKKMGRPWEMGKSFDESAPITALRPAAEIGHPAKGAIVLK
                     VNGQVRQQGDLAMQIWSVPEQIAYLSNLITLQPGDLIFSGTPAGVGPVKPGDKLEGHV
                     DGVGDLTVTYRS"
     gene            4850..6250
                     /locus_tag="VFV55_08380"
     CDS             4850..6250
                     /locus_tag="VFV55_08380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX4944356.1"
                     /translation="MKPLSGLDTAFLCLETPETPMHVGSLHLYRVQSRSRAGYFERAK
                     KHIASRLHLAPVFTRRIASLPFDIASPVWVEDREVDLDHHIRRIRLRKPGTMAALEAT
                     VGRLHGELLDRNRPLWMFYVIEGLATGEVAWYSKIHHAALDGAAGVKLAEAILDASSK
                     PRKVAGPAKRKLEKTPGVAELLGAAFTKSIAEYGKLVRSIPEGARVLKESLRAISSLA
                     PPGKGSARGMPIGPATAFNIPIGGKRAFATASIPLTEVKTIARRHDAKVNDVVLALVS
                     GAVRRYLITKNALPRKPLVAAMPVSLRAEGDSIYSTRATLVLANLATHLAHPLARLEA
                     IRASAGNAKAITVKAKSIIPMDFPPLGAPWLLAAIARAYGYAQGVRSFPPLANLVVSN
                     VPGPQAPLFLAGARMLTWWPVSIVEHGLGLNVTVQSYSGSLDFGLVAACDAMPDVRKL
                     AAALYDAHEELKNARA"
     gene            6247..7047
                     /locus_tag="VFV55_08385"
     CDS             6247..7047
                     /locus_tag="VFV55_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944357.1"
                     /translation="MNQTHDLEHWYDYGLMRAPGMLRLALEWRAPWEYGASVLFRPLV
                     EMAPKGDGHPVLLFPGLLASDRTTAPLRGFLKEQGYAPYGWELGTNRGPREGVMETCR
                     ARLATIHKRHKRKVSLIGWSLGGIYAREFAKETPRAVRLVITLGTPFTGHPKATNAWR
                     LYEKVTGHKIGSAELHVPLRVPPPAPTTSIFSRTDGIVAWQCSVGRPGPLHENIEVEA
                     SHLGLGFNPLAWYAIADRLAQPEGKWQPFDRGGLRSWLYRDPARPVWF"
     gene            7055..7939
                     /locus_tag="VFV55_08390"
     CDS             7055..7939
                     /locus_tag="VFV55_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4944358.1"
                     /translation="MPTVSANGISLAYEVAGEPGAPVMVLVMGLGMSLVFWPDAFVEG
                     LVNAGFRVLRFDNRDCGLSQRIAAGHDTPIPVAMARSMMGLEVHAPYNLADMAGDALG
                     LLDALSIGKAHVVGASLGGMVAQVLAARHPERVSSLTSIMSSSGNPFVSMARPRALGA
                     ILHRPNDPGDPASVTRHLMHVMGVIGSPGYRADAGALREICERAATRGYDIHGTARQM
                     LAMLASGDRRSELATIRVPTLVIHGTDDPLVPRAAGREVARIVPGAKLLEFDGMGHDL
                     PAPLIPELVAAIAGHCRP"
BASE COUNT         1334 a         2727 c         2739 g         1242 t
ORIGIN      
        1 atcgttgggc atgggtgctc ggggcggttg ctgcgctgcg gaaaggagct attttggcgc
       61 attcccgggt tttggggacg gttctccgga accgtcccca aaacctgtcc cccggcaagc
      121 cctagaaatc ggcggcttgc gtcgcggctt ccgtcgcggc ttcccgcgcg cgcctgagac
      181 gctcggcgcg acggtgctgc cagagggcga acaccaccga tgccaccgaa aggccgagca
      241 gcgcgccgga aatcgggcgc gtcacgaata gccacgggtt cgcgtcggtc atgatggagc
      301 gcaccaggtt ggtctcgatc tggtcgccga ggatcacgcc gaggatgaag ggcgccaggg
      361 gaattccggt ctttaccatc aggtagccca gcaccccgaa ggccagcagg acgtacacgg
      421 acgacatcga gttgcccagc gcataggccc cgaccacgca aagcaccagc acgaccggga
      481 acaggatcga cttcggcacg gtcacgatcc gcagcgccca gcgcgccccc agccccatga
      541 tcaggaccat gatcgggtgc gcgagcaggt aggccgcgaa gatcccgtac gcgatcgtcg
      601 ggtgctgcgc gatgaagagc ggtcccgact ggatgccgtg gatcgtgagc gcgcccagca
      661 tgacggcggt cacggcgtcg cccgggatgc cgaaggccat gatggtgacg agcgagccgc
      721 cgacgttggc gttgttcgac gattcggacg cgatgatgcc ctcgggaatg ccctggccga
      781 attgctcggg gcgcttcgag aatttctttg cctggtcgta ggccatcatg ttggccgcgc
      841 tgccgccgat tgccggcagc acgccgatcc acacgccgat gatcgacgac cacagcagca
      901 ggaaaggcct cgagaggatc tcgccgatca ccctgagctg cgacaccttg aggttcgccg
      961 ccgcgtcgat cgcccgcgcc gcgctccccg agccgcccgg cctctccacc tccgacatga
     1021 tctgcgcaaa ggcgaacacg ccgatcagca cgggcaggaa cggcacgccg ccttcgagga
     1081 actcgatgcc gagggtgagc cgcggcgtgc cgagcatcgg gtcgtttccg aggaccgtga
     1141 cgatgagccc gatcattccc gagatgagcc ccttcagcag cgacgcctcg accagccccg
     1201 ccaccatgga gagcgcgagc acgaagagcg agaagtactc ccagggaccg aactcgaggg
     1261 cgaacgcggc gagcggcccc gtggcggcga tgagaaacat tccgcctatc aggccgccga
     1321 agaacgatgc ccagatgccc agccagatcg cgcgtcccgg ttcgccgatg accgccatcg
     1381 gatggccgtc gaaggtcgtc gcgaccgccg agggcgttcc gggaattccc aggagcaccg
     1441 ccgtgacgag accgccggcc gagccgccga cgtagagccc ggtcatcgcc gccaggccct
     1501 gcaggggatc gagcccgaag gtgaacggca gcgccacgat gatcgtcatc gtgatggtga
     1561 cgccgggcag cgcgcccccg accgagccgg cgagcgtacc gaggaacagc gggacgaggt
     1621 acttgaactg cgcgatgttg acgagcgcga tgccgagttg ctcgtacatc tctagaaatc
     1681 cgtccagctg ccgcgcggca acaggacctt caggtacgcg tcgaacgtgt agtacgtgcc
     1741 ggcggtcgcg acgaaggcga cgagcgcgac gagtgcccag cggcgcattc cgcgcggcgg
     1801 ctcgagcgcc gcctgcatgg cggccacgaa ggcgaaggtg gcgatcctga atcccgcgta
     1861 cggcagcgcc acgacgtagg cgccgaagag ggcgaacagc gcaacgacca tcgcgtagcg
     1921 caggcggggc cgtggcgacg gcggggcgcc gcccgcgcgc cggtgcgcca gtacgtcggc
     1981 cgccacgagg agcgcggcga gagccgccgt gatgcccagc acgatgcgcg gatagaacgc
     2041 cgggccgatc ggcacgaggg gattctgctc gagcccgagc gtcgcccaaa agagcgcgac
     2101 gctcgcggcg agcacgacga ggccggtgaa tccgtcgcga cccatgttcg ctgcggtggc
     2161 gggccggtca gcgcttcagc aggccgaggt ccttcgcgag gtcgtggttg atcttgtcgt
     2221 tgccctgcag gaacccggtg tattccttcg gcccgaggaa gttcaccagc gttccctggc
     2281 tcttcatcgc ctcggcgaaa ccgggctcct tcgcggcggc ggcgcaggcc gactgcagct
     2341 tggccatcac gtcggcgggc gttgccttcg gtgcgatcag tccgcgattc accgcatagg
     2401 tgacgtcgac gcccagttcc ttcagcgtcg ggacgtcggg tacctcggcg ttacgcttgt
     2461 ccgtggcgat cgcgaggaag cgcatctggc cagcatcggc cttgcccttc tgggtcaggt
     2521 tcgattcgcc catgtcgata tggctgccga ggagcgcgtt catgcgcggc gcggttccct
     2581 cgtacggaac gtacttgaac ttgatgccgg ccgccttttc gaccatcgcg gggaagaaat
     2641 ggctcgtgga ccccagcgag gcgcccacga tgatctggcc gggccgtgcc ttggcgtcag
     2701 ccagcatggc cttcgcatcc ttccacgtcg acttcgggga cgcggtgagg atcgagggcg
     2761 tcgaggccac gaggcagatc ggctggaaat cggtccacgc cacgtcggcc acgcccgtgt
     2821 agtacgtcga gtggatggaa tcgtgcaccg cgaagatggt atagccgtcc gcgggggagt
     2881 ccttggcctc cttggcgccc ttcgtgccgg aggcgccgcc cacgttggcg atgacgatcg
     2941 ttccgccgag gtgcttctgc aggaagggct ggaacgcgcg atagaccacg tccgtgtcgc
     3001 cgcccgcggc ccagggcacg atcagcttta ccgggcggtc gggataggcg gctgaagcgg
     3061 aaaagatgcc cacccaggcg agggtggcga gcagggcgag gaagcgggtc atgattggaa
     3121 tcctccggga ttatcgtggt ggtcgtcgtg gcgctccgcg catattagac tgccgcatcg
     3181 caccactcaa acggagtttc ccccaaatgc gcatcggcct gctcggcggc ggcgtgatcg
     3241 cgaggctttt cctggaccac gtgcacggcg cggaaggcga tgcgcgggtg gtggccatcg
     3301 cgggccgcag cctttcctcg cgttcccggg ccctcgccga cgaacgcggc gtgcctttcg
     3361 tcgtcggtgc cgcggcgctc gtcgcgactc gtcccgacgt ggtcgtcgag gccgcctccc
     3421 acgacgcggt gcgcgaacac ggcgagacgc tcctggggag cgggatcgcg atgatcgtgc
     3481 tctcgggcgg ggcgctcgcc gacgacgaat tgcgcggccg tctcgaacgc gcggccgcca
     3541 ggggtcgcgc gctgctctac gttccctcgg gcggaatcgg cgggctggac gcgctcaagg
     3601 ccgcgtgcgc cgcgggcgtg gaagaagtga gcatcagcgt gacgaagccg ccggcggcgt
     3661 ggaaggacat cgcttacgtc gagcggctcg gcatcgatct cgatcggctc gcggggccgg
     3721 tgaccctgtt cgagggcagc gcccgcgagg gcgtgccgca ctttccggcg aacgtgaaca
     3781 tcgcggcggt gctcgcgctc gccggcgcgg ggttcgaccg cacgcgcctg cgggtggtcg
     3841 cggatcccgc actgcggttc aatacgcact tcatccgcgt gaagggcagg accggcacca
     3901 tcgacatgcg cttcgaaagt gttccatccc ccgacaaccc gaagaccgcc atgctcgcgt
     3961 gctacagcgc gctcgcggcg ttccggcagt tcgcctcgcc ggtgcgatac ggaacgtagg
     4021 cgtctagaat gacctcgtga ccggaggaat tccggacatg agagtctcga aggccattgc
     4081 aggcaatccg gaccaataac gaaagggaga aatgggaaaa tacgtcatcg acatcggtcc
     4141 cctgccttcg ctgcccgtcg ccggcagcga tgcgcgcttc cccgtgggcc gcatctactg
     4201 cgtcggccga aactatgccg agcacgcgcg cgagatgggc cacgacccgg atcgcgaacc
     4261 gccgttcttc ttcatgaagg ccgccaactc catcgtggag aacaacgcca cgatctccta
     4321 cccggtcggc acgaaggatg tccaccacga gatcgagatg gtggtggcga tcggcaaggg
     4381 cgggaagaac attccggtcg agaaggcgct ggaccacgtc tggggctacg gcgtggggct
     4441 ggacatgacg cgccgcgaca tccagggcga ggccaagaag atgggccgcc cgtgggaaat
     4501 gggcaagagc ttcgacgagt cggcacccat cacggcgctg cggcccgccg ccgaaatcgg
     4561 gcatcccgcc aagggcgcca tcgttctcaa ggtgaacggc caggtgaggc agcagggcga
     4621 cctggcgatg cagatctgga gcgtgcccga gcagatcgcc tacctctcga acctgatcac
     4681 gctgcagccc ggcgacctga tcttctccgg cactcccgcc ggcgtgggcc ccgtcaagcc
     4741 gggcgacaag ctcgagggcc acgtcgacgg cgtcggggat ctcaccgtca cctacaggag
     4801 ctagggagtt gggggacagg gtccccttcc ccgtgctctc gccaccccgt tgaagcccct
     4861 ctccggcctc gacaccgcgt ttctctgcct ggagacgccc gagaccccga tgcacgtggg
     4921 gtcgctgcac ctgtaccggg tacagtcgcg cagccgcgcg ggctacttcg aacgcgcgaa
     4981 gaagcacatc gcaagccgcc tgcacctcgc cccggtcttc acgcgccgca tcgcctcgct
     5041 tcccttcgat atcgcgagcc cggtgtgggt cgaggaccgg gaagtcgacc tcgaccatca
     5101 catccgccga atcaggctgc gcaagcccgg cacgatggcc gccttggagg cgaccgtcgg
     5161 gcgactgcac ggcgagctgc tcgaccgcaa ccggccgctg tggatgttct acgtgatcga
     5221 ggggctcgcg accggggaag tcgcgtggta ctccaagatc caccacgcgg cgctggatgg
     5281 cgcggcgggc gtgaagctcg ccgaggcgat cctcgatgcg tcgtcgaaac cgcgaaaggt
     5341 ggcagggccg gcgaagcgca agctggaaaa gacgcccggc gtcgcggaac tcctgggcgc
     5401 cgcattcacg aagagcatcg cggaatacgg caagctcgtg cgctccatcc cggaaggggc
     5461 tcgcgtgctg aaggaaagcc tccgcgcgat ctcctctctc gcccctccgg gcaagggaag
     5521 cgcgcgcggc atgcccatcg gcccggccac cgccttcaac atcccgatcg gcggcaagcg
     5581 cgcctttgcc acggcgtcga ttccgctcac cgaggtgaag acgattgcca ggcgccacga
     5641 tgccaaggtg aacgacgtgg tgctcgcctt ggtgagcggc gcggttcgcc gctacctaat
     5701 cacgaagaat gccctgcccc gcaagcccct cgtcgccgcc atgccggtgt cgctgcgcgc
     5761 cgagggcgat tcgatctact cgacgcgcgc gaccctggtg ctcgccaacc tggccaccca
     5821 ccttgcccat ccgctggccc ggctggaggc catccgtgcc tcggcgggca acgcgaaggc
     5881 gatcacggtg aaggcgaagt cgatcatccc gatggatttt ccgcccctcg gcgcgccctg
     5941 gctgctggcg gccatcgcac gcgcctacgg ctacgcacag ggtgtcaggt cctttcctcc
     6001 tctcgccaat ctcgtcgtct ccaacgtgcc cggcccgcag gcgccgctct tcctcgccgg
     6061 cgcgaggatg ctcacctggt ggccggtatc gatcgtcgag cacgggctgg gactcaacgt
     6121 caccgtgcag agctattccg ggtcgctcga tttcggtctc gtggccgcat gcgacgcaat
     6181 gcccgatgtg cgcaagctcg ccgccgccct gtacgatgcg catgaagaac tgaagaacgc
     6241 acgcgcatga accagacgca cgacctcgag cactggtacg actacggact gatgcgcgcg
     6301 ccggggatgc tgcgcctcgc gctggaatgg cgggccccgt gggaatacgg cgcgtcggtg
     6361 cttttccgcc ccctcgtcga gatggcgccc aagggagacg gtcacccggt gctgctcttc
     6421 cccggcctgc tggcctccga ccgcacgacc gcgccgctgc gcggtttcct caaggaacag
     6481 ggctacgccc cctacggctg ggagctgggc acgaacaggg gcccgcgcga aggcgtgatg
     6541 gaaacctgcc gcgcgcgcct cgccacgatc cacaagcggc acaagcgcaa ggtttcgctc
     6601 atcggctgga gcctcggcgg catctacgcg cgggagttcg ccaaggaaac gccgcgcgcc
     6661 gtgcggctgg tgatcacgct ggggacgccg ttcaccggcc atcccaaggc caccaatgcc
     6721 tggcgcctct acgagaaggt caccggccac aagatcggct cggcggaatt gcacgttccc
     6781 cttcgcgtgc cgccgcccgc gcccaccacg tccatcttca gccgcacgga cggcatcgtc
     6841 gcatggcaat gcagcgtcgg aaggcccggg ccgctgcacg agaacatcga agtggaggcg
     6901 agccacctgg gcctgggatt caatccgctc gcgtggtacg cgatcgccga ccggctcgcg
     6961 caacccgaag gaaagtggca gccgttcgat cgcggcggcc tgcgttcgtg gctctaccgc
     7021 gatcccgcgc gccccgtatg gttctagcca cgcgatgccg accgtaagcg cgaacgggat
     7081 ttcgctcgcg tacgaggtgg ccggcgagcc gggcgccccg gtgatggtcc tggtgatggg
     7141 gctcggcatg tccctcgtgt tctggcccga tgcattcgtc gaagggctcg tgaacgcggg
     7201 attccgcgtg ctgcgcttcg acaatcgcga ttgcgggctc tcgcagcgca tcgccgcggg
     7261 gcacgacacg ccgattcccg tggcgatggc ccgctcgatg atgggcctgg aggtgcacgc
     7321 gccctacaac ctggcggaca tggccggcga tgccctgggc cttctcgatg cgctttccat
     7381 cggcaaggcg catgtggtcg gcgcatcgct gggggggatg gtggcgcagg tgctggcggc
     7441 acggcatccg gagcgtgtct cgagcctcac ctccatcatg tcctcgagcg gcaacccgtt
     7501 cgtttcgatg gcgcgcccgc gtgcgctcgg cgcgatcctg catcgtccga acgacccggg
     7561 ggatcccgcc agcgtcacca ggcacctgat gcacgtgatg ggcgttatcg gcagccccgg
     7621 gtatcgggcc gacgcggggg cgcttcgcga gatttgcgaa cgtgctgcga cgcggggcta
     7681 cgacatccac gggaccgcgc ggcagatgct cgcgatgctc gcctcgggag atcgccgctc
     7741 cgagctcgca acgattcgcg tgcccacgct cgtcatccat ggcaccgacg atcccctcgt
     7801 ccccagggcg gcggggcgcg aggtggcgcg catcgttccc ggcgcgaagc tcctcgagtt
     7861 cgacggcatg ggccacgacc ttccggctcc gctcatcccc gagttagtcg ccgccatcgc
     7921 cggccactgc cgtccgtaat ttgtaccggg tacatgtacc cggtacatgt acccggtacc
     7981 gtcccctgga gggtcgccgg cgggcgacag gggcgaattc gtcggaacct gaggcgcgat
     8041 gc
//
LOCUS       DASUEV010000259         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_82892, whole genome shotgun sequence.
ACCESSION   DASUEV010000259 DASUEV010000000
VERSION     DASUEV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82892"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFV55_08395"
     CDS             complement(<1..971)
                     /locus_tag="VFV55_08395"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX4944359.1"
                     /translation="MCGIVGVVARSPVNQFLYDGLTVLQHRGQDAAGIVTAEGHSFHM
                     HKARGLVRDVFRTRDMRSLVGTMGIGHCRYPTAGSASSVAESQPFYVNSPFGIVLGHN
                     GNLVNAEELRKSLFLEDRRHINTNSDSEVLLNVLAHEIHDACDGGHRLTPDAIFNAVG
                     GVHRRVKGAYAVVAMIAGYGLLAFRDPKGIRPLIIGRADTEKGVEFIVASESVALDTL
                     GFKAMRDVAPGEAILIDQSGNLYSRQCAEETALNPCIFEFVYLARPDSVIDGVSVYES
                     RRNMGKSLADKILASGITPEIDVVIPIPDSSRPSALEVATKLGKPYR"
     gene            complement(1091..1630)
                     /locus_tag="VFV55_08400"
     CDS             complement(1091..1630)
                     /locus_tag="VFV55_08400"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX4944360.1"
                     /translation="MQGNPTSEQAELRRKGRQRLIGAVAIALIAVVFVPMLLDPEPRR
                     ERVEPPLAIPPKDGVPPLAPAARLPEPAVQPETAKVAPADAPAPPVKQEAKSVPSSPP
                     LEGFAVQVGAFKEDAKLAQARDKVAAARLAHYTERLAGASGELTRLRAGPFPTREAAD
                     KAAAQLKRAGLDARVVTLP"
     gene            complement(1643..>2467)
                     /locus_tag="VFV55_08405"
     CDS             complement(1643..>2467)
                     /locus_tag="VFV55_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX4944361.1"
                     /translation="DAVNIVDADCSIVSSVDFDHQAWLGDTREAIGLEKAHIFRAARP
                     AFFGDTDPPASLVGHATAIGADLQVLGRDFRAVPHERQWDFEGRRGARRALPMPALRG
                     PWQLANAATALAALDEVSDRFPVSLGEVKRGLTAVRLEGRLQVLPGRPTVVLDVAHNP
                     HAARALARGLGEMGFHENTIAVFAMLADKDIGAVIDAVSPRVDRWHVATVANERAASA
                     SQVAGLLAARGLADRTRVFATVPLAYEAALREAGPNDRIIVFGSFHTVAGVLETLR"
BASE COUNT          377 a          827 c          879 g          384 t
ORIGIN      
        1 tcgcgatagg gcttgccgag cttcgtcgcg acttcgagcg cgctcggccg gctggaatcg
       61 gggatcggga tcacgacgtc gatctcgggt gtgatccccg aggcgagaat cttgtcggca
      121 aggctcttgc ccatgttgcg ccggctctcg tacaccgaaa cgccgtcgat caccgaatcg
      181 ggccgcgcca ggtagacgaa ctcgaagatg cacgggttca gcgccgtttc ctcggcacac
      241 tggcggctgt agaggttgcc gctctggtcg atgaggatcg cctcccccgg cgcgacgtcg
      301 cgcatcgcct tgaagccgag ggtgtccagc gccacgctct cggaggccac gatgaactcc
      361 acacccttct ccgtgtcggc tcgcccgatg atgagcgggc ggatgccctt cgggtcacgg
      421 aaggcgagca atccgtagcc ggcgatcatc gcgaccacgg cataggcgcc cttcacgcgc
      481 cggtgcacgc cgccgacggc gttgaagatg gcgtccggcg tcaggcggtg cccgccgtcg
      541 cacgcgtcgt ggatctcgtg cgcgaggacg ttcagcagga cctcggaatc ggaattcgtg
      601 ttgatgtggc ggcgatcctc gaggaacagc gacttgcgca gttcctccgc attcaccagg
      661 ttgccgttgt gccccagcac gatgccgaag ggcgaattca cgtagaacgg ctgcgactcc
      721 gcgacggacg atgcggagcc ggcggtggga tagcggcagt ggccgatgcc catggtcccc
      781 acgaggctgc gcatgtcgcg cgtgcggaag acgtcccgaa ccagcccgcg ggccttgtgc
      841 atgtggaagc tgtgcccttc ggccgtgacg atgccggccg catcctgccc gcggtgctgc
      901 agcaccgtga gcccgtcata gagaaactgg ttgacggggc tgcgcgcgac gactccgacg
      961 attccgcaca tgcttcaggc atccctcgat ccggggcacg tgaccccgcg atagtgtatg
     1021 cgttttgcct cgggccgtgg atcgcccggc ctgcgtaaca tgggggcggc gccggccgat
     1081 cgcaagcgcc tcacggcagc gtcaccacgc gcgcgtcgag ccccgcgcgc ttgagttgcg
     1141 cggccgcctt gtccgccgct tcccgagtgg gaaacggtcc ggcgcgaagg cgcgtgagtt
     1201 ccccggatgc gccggcgagc cgctcggtgt agtgcgcaag gcgcgcggcg gcaaccttgt
     1261 cgcgcgcctg cgcgagcttg gcgtcctcct tgaatgcgcc tacctgtacg gcgaagccct
     1321 cgagtggcgg cgacgaaggg acggatttcg cttcctgctt caccggcggg gcgggtgcat
     1381 cggccggcgc gactttcgcc gtttcgggct gcaccgccgg ctcgggcaac ctggccgccg
     1441 gagcaagcgg cggcaccccg tccttcgggg gaatggcaag cggcggttcg acgcgctccc
     1501 ggcgcggttc cggatcgagc agcatcggga cgaacacgac ggcgatgagg gcgatcgcca
     1561 cggcgccgat caggcgctgg cggcccttgc ggcgaagctc agcctgctcg gaggtggggt
     1621 tgccctgcat gtcggttggc ggtcatcgca gcgtttcgag aacacccgca acggtatgga
     1681 acgagccgaa gacgatgatt ctatcattcg gacctgcctc ccgaagggcg gcctcgtagg
     1741 cgagcggcac cgtcgcgaat acgcgcgtgc gatcggccag cccgcgcgca gccaggagac
     1801 ccgccacctg cgaggccgat gcggcgcgct cgttggccac ggttgccacg tgccagcgat
     1861 ccacgcgcgg cgagacggcg tcgatcaccg cgccgatgtc cttgtcggcg agcatcgcga
     1921 agaccgcgat ggtgttctcg tggaagccca tctcgcccag cccccgcgcg agagcgcgcg
     1981 cggcgtgggg attgtgcgcg acgtcgagga ccacggtcgg ccggccgggc agcacttgca
     2041 gccggccctc caggcgcacc gcggtcaggc cacgcttcac ctctccgagc gagacgggga
     2101 aacggtccga cacctcgtcg agggccgcaa gcgcggtggc agcgttcgca agctgccacg
     2161 gcccgcgcag ggccggcatg ggcagtgcgc gcctggcgcc gcgccggcct tcgaaatccc
     2221 attgccgctc gtggggaacg gcccggaagt cgcggccgag cacctgcagg tccgcgccta
     2281 tcgcggtggc gtggccgacg agggaagccg gaggatcggt gtcgccgaag aaagccgggc
     2341 gcgcggcacg gaagatgtgg gctttttcaa ggccgatcgc ctcgcgggta tcgcccagcc
     2401 aggcctggtg gtcgaaatcc acgctggaga cgatcgagca gtcggcatcg acgatgttga
     2461 ccgcgtc
//
LOCUS       DASUEV010000260         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1570205, whole genome shotgun sequence.
ACCESSION   DASUEV010000260 DASUEV010000000
VERSION     DASUEV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570205"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFV55_08410"
     CDS             <1..202
                     /locus_tag="VFV55_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX4944362.1"
                     /translation="VSRYSSLGALTASLFAPAAVYLLRGAGPLLVATCAICAVLVWRH
                     EANIRKLLRGEESRIGEKKAAT"
     gene            199..771
                     /locus_tag="VFV55_08415"
     CDS             199..771
                     /locus_tag="VFV55_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4944363.1"
                     /translation="MTPIDSDRAGDDLDFTRPAAPPPPPTPGQLEIAGKEASVGGAEL
                     RKAGYYVAIARRAKAKVPPRNGERHALLVVEDDPDLSSLLAEIFSVAGFEVRQAANRT
                     QINAEVNRPILPDLILLDIVLPDADGMQILARLRAHPKFARMPVIMMTGKAESGDVKA
                     GLAAGADGYVSKPFKMSALVAAVNLVLGRS"
     gene            complement(768..1130)
                     /locus_tag="VFV55_08420"
     CDS             complement(768..1130)
                     /locus_tag="VFV55_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX4944364.1"
                     /translation="MLPDLVLLLHAAFVLFVVGGLVAIWVGAALGSGWARNPWFRGLH
                     LAAIAFVVAQSVLGYTCPLTIWEDALRGAATGEGFIQRWVRALLYWNAPPWVFTAIYV
                     AFGALVAGTWWRVPPRRG"
     gene            1259..>3219
                     /locus_tag="VFV55_08425"
     CDS             1259..>3219
                     /locus_tag="VFV55_08425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX4944365.1"
                     /translation="MHRYISRALAATLLLGFGAGAFAQKSAKDIPVETFLKRGQYGSM
                     ILSPDGSRLAALSPLKGRNNLVVLDLAKRTRNVITSFEAVDVANFSWINNDRVCFSVA
                     DGQEVSGRFTFRGSYCINQDGSGVRDFSEFARRGMTLYARTQDGSAKAYVGMNSRTRD
                     SQDVYLLDTASGRSELLTFDSPGRVISWVLDWNNVPRVAVSSPERESNDALAWTQVWY
                     REGKDAKWEKLWEFSQLAEGARSDDYAPLAFDFDNTTLYVSHNAGRDKRAIYKYDTKA
                     RKMGELVFEHPLIDVSGGLVFSRAQKKLLGIRYSAEKPSVKWFDPAMERLQSQLDSTL
                     KGTFNSFALAPDNENLALIFAYSDTDPGMYHLLDRAKPSIESLVRTREWLDPALMSER
                     RFIRYKARDGLEIPAWVTIPRGSSGKNLPLIVHVHGGPWVRVYGGGFGLEAQFFASRG
                     YAVLEPEPRASTGFGKKLLRSGYKQWGQAMQDDINDGALYLVKEGIADARRMCIFGGS
                     YGGYAASIGPARDPDFWKCAAPYVAVTDLGLMQTIAYSDISWASDFLQTDFKKMVGDS
                     DADREMFAKTSAVQLAGRVKAPIFLSMGSDDVRVPITHGEKFRDALERNGKKIEYVVY
                     PGEGHGYNKDEHRFDFYRRLEKFFAENLK"
BASE COUNT          574 a         1085 c         1060 g          500 t
ORIGIN      
        1 cgtgagccgc tactcgtcgc tgggcgcgct caccgcgtcg ctcttcgcgc cggcggcggt
       61 gtacctgttg cgcggcgccg gcccgctcct cgtcgccacc tgcgccatct gcgcggtgct
      121 cgtgtggcgc cacgaggcca atatccgcaa gctgctgcgc ggcgaggaga gccgaatcgg
      181 cgagaagaag gccgcgacat gaccccgatc gattccgatc gcgccggcga cgacctggat
      241 ttcacccggc cagccgcccc gcctccgccc ccgactccgg ggcagctcga aattgccggc
      301 aaggaggcgt cggtgggcgg ggcggaactg cgcaaggcgg gctactacgt cgccatcgcg
      361 cggcgagcca aggccaaggt gccgccgagg aacggcgaac gccatgcctt gctggtggtg
      421 gaggacgacc cggatctttc cagcctgctc gcggagatct tcagcgtggc gggcttcgag
      481 gttcggcagg ccgccaatcg cacgcagatc aacgcagaag tgaacaggcc catcctgccg
      541 gacctcatcc tgctcgacat cgtgctgccg gatgccgacg ggatgcagat cctcgcgcgc
      601 ctgcgcgccc accccaagtt cgcgcgcatg ccggtcatca tgatgaccgg caaggcggag
      661 tccggcgacg tgaaggccgg gctcgcggcc ggggcggacg gctacgtgag caagccgttc
      721 aagatgagcg cgctcgtggc cgcggtgaac ctcgtgctgg gaagaagcta gccgcgccgc
      781 ggcggaacgc gccaccaggt cccggcgacc agcgccccga acgcgacgta gatcgcggtg
      841 aacacccacg gcggcgcgtt ccagtagaga agcgcgcgaa cccagcgctg gatgaacccc
      901 tcgccggttg cggcaccgcg aagggcatcc tcccagatcg tgagcgggca ggtgtaaccg
      961 agcaccgact gcgccaccac gaatgcgatg gccgcgagat gcagaccgcg gaaccacggg
     1021 ttcctcgccc agccgctgcc gagcgccgcg cccacccata tggcgacgag tccgcccacc
     1081 acgaagagga cgaaggcggc gtgcaggagg agcaccaggt cggggagcat gggcgcggcg
     1141 gccttcccgg ccgcggctgc agccgcggcc tgcggacata gccactataa tctcgctcgt
     1201 cgtgggagtc ccatgcggac caccattccg gcccatccac ccctcgaggc ctgccaccat
     1261 gcaccggtac atttcgcgcg ctctcgccgc gaccctcctc ctcggattcg gcgccggtgc
     1321 cttcgcgcag aagtcggcga aggacattcc cgtcgaaacc ttcctcaagc gcgggcagta
     1381 cggctcgatg atcctctctc ccgacggttc gcggctcgcg gccctttccc cgctcaaggg
     1441 ccgcaacaac ctcgtcgtcc tcgacctcgc caagcgcacg cggaacgtga ttacctcctt
     1501 cgaggccgtt gacgtcgcga atttcagctg gatcaacaac gaccgcgtct gcttctcggt
     1561 ggccgacggc caggaagtca gcggccgctt caccttccgc gggtcgtact gcatcaacca
     1621 ggatggcagc ggtgttcgcg atttcagtga attcgccagg cgcggcatga ccctgtatgc
     1681 ccgtacccag gacggctcgg caaaggccta cgtgggcatg aacagccgga cgcgcgattc
     1741 gcaggacgtg tatctcctcg atacggcgtc cggccgctcc gagctcctca cgttcgattc
     1801 gccgggccgc gtcatttcct gggttctcga ctggaacaac gtgcctcgcg ttgccgtgag
     1861 cagccccgaa cgcgagagca acgatgcgct ggcgtggacg caggtgtggt atcgcgaggg
     1921 caaggacgcg aagtgggaga agctctggga attctcgcag ctcgccgagg gcgccaggag
     1981 cgacgattac gcacccctcg cgttcgactt cgacaacacg acgctctacg tctcgcacaa
     2041 cgccgggcgc gacaagcggg cgatctacaa gtacgacacg aaggcacgca agatgggcga
     2101 gctcgtcttc gagcatccgc tcatcgacgt gagcgggggg ctcgtgttca gccgcgccca
     2161 gaagaagctg ctgggcatcc ggtacagcgc cgagaagccg tccgtgaagt ggttcgaccc
     2221 ggcgatggag cggctgcaga gccagctcga ttcgacgctg aaggggacgt tcaacagctt
     2281 cgccctcgct ccggacaacg agaacctcgc cctgatcttt gcctactccg acacggaccc
     2341 ggggatgtat cacctgctcg accgcgcgaa gccctccatc gagagcctgg tgaggacgcg
     2401 cgagtggctc gaccccgccc tcatgtccga gcgaaggttc atccggtaca aggcgcggga
     2461 cgggctcgag atccccgcgt gggtcacgat ccccagggga tcctcgggca agaacctgcc
     2521 gctgattgtg cacgtccatg gcggtccctg ggtgagggtg tacgggggtg gcttcgggct
     2581 ggaggcgcaa ttcttcgcct cccgcggcta cgcggtgctg gaacccgaac cgcgtgcctc
     2641 gacgggtttc ggcaagaagc tgctgcgctc cgggtacaag caatgggggc aggccatgca
     2701 ggacgacatc aacgacgggg ccctgtacct ggtgaaggaa ggcatcgccg atgccaggcg
     2761 catgtgcatt ttcgggggca gctacggcgg ctacgccgca tcgatcggcc ccgcccgcga
     2821 ccccgatttc tggaagtgcg ccgcgcccta cgttgccgtg accgacctcg ggctgatgca
     2881 gaccatcgcc tattccgaca tcagctgggc cagcgacttc ctgcagaccg acttcaagaa
     2941 gatggtcggt gattccgacg cggatcgcga gatgttcgca aagacctcgg cggtccagct
     3001 cgcgggccgc gtgaaggccc cgatcttcct ttccatgggc tcggacgatg tgcgggttcc
     3061 gatcacccac ggggagaaat tccgcgacgc gctggaaagg aatggcaaga aaatcgagta
     3121 cgtggtctat ccgggcgagg ggcacggcta caacaaggac gagcaccgct tcgacttcta
     3181 ccggcggctg gagaagttct tcgccgagaa cctgaagta
//
LOCUS       DASUEV010000261         8819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1449871, whole genome shotgun sequence.
ACCESSION   DASUEV010000261 DASUEV010000000
VERSION     DASUEV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449871"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VFV55_08430"
     CDS             complement(<1..1603)
                     /locus_tag="VFV55_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX4944366.1"
                     /translation="MSPGEGAVFGIGLPHAGAVPGDVTGMGFKAFNLQRMAAAGMPVP
                     QAFVLGTDWCRLHQRDPAAARQPLRVALVEWTRRLEQACGLSFGGERRPLLVSVRSGA
                     PVSMPGMMDTVLNIGLCDRTVRGLLRSTGNPRLVWDSYRRLVQQYAQVVHGADGAAFD
                     ELAAAAVSSAGVAGARELDFESLVMLNAGSLERFADITGEPFPQDPREQLEAATVAVL
                     DSWHSARAEEYRRLHAIDDAIGTAVTVQRMVFGNAGGTSGSGVGFTRDPSTGENRLYL
                     DFLFNAQGEDVVSGRHAVTDTAALPVALPEVAQRLDELRHALETTFGDAQEFEFTVQD
                     GRLYLLQSRAAKRTPWAALRMAVEQVREGLATPAQALARLDGIDLAAIERRRVADEGE
                     AALAVAEPASIGLAVGAIALDAKACERLAARGRPAILVRSDTSTEDIAGIAIAAGVLT
                     VRGGRTSHAAVVARQMGKVCLVGCATLRIDEKARAIVLGTRTLREGETICLDAERGRV
                     LEGEPEVVVERPEGLLSEVARWRADA"
     gene            complement(1600..3537)
                     /locus_tag="VFV55_08435"
     CDS             complement(1600..3537)
                     /locus_tag="VFV55_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944367.1"
                     /translation="MEKSRIVEALGEEGLRLPALLNEALAANDRAKYLFTVLQVAQGH
                     AERPGSAAPELRAEREAAGLAAAGFDEVAAAATKTGDGRYYMPGVERLCTRLRAEIDA
                     MLAPLLASGEDAAAYDERRRRLDEVPWCGADDQITGDQIGAIASGGHEGTDSVHRLVM
                     DMHKALNGLQARISSEIIEGAHAYGIAPGDRPLVAAFMRGVNRTAALKFDHPGLGTTA
                     TRSGDRLVLQNDIGTTDAHVLVVHVEANRATLTYTDVHMQRLVFFQGLFDARRVEWAD
                     TLSRTDRSMADGVYHLCVGSFTAASDAELEDYLAFMGSRLVFLIDWNRARKRLRLLLP
                     RKEVAELLKWAADNDHGHMAFLRAGGEQMIFDSLAFVSRAPSSFGARLDDVLGHDAAV
                     KFMRYVMRTCAQGLQGGRPEGLVRDEVRAELVSYFRSAQESVLDVAAEHAAFALEIAS
                     GIRDALLSAGAPSAGEAFARNARRAKAWEHRADELVNAARAQARHSERARTYRDLIEV
                     ADDIVDELEEAAFHLALMPHDGRGEALYAALRPLAALAVESVQEYLKAVETARTLHRG
                     SPREDTQDFLEAVHRIMTLERRGDEVHRSAKSLVPAQAGEFAGLFGFIECARNLEGAT
                     DALMHTALKLRDAVLGELVVQ"
     gene            3650..4429
                     /locus_tag="VFV55_08440"
     CDS             3650..4429
                     /locus_tag="VFV55_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380881.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX4944368.1"
                     /translation="MAPLKIGLSARLLYPDPRRTFLPTKCIQYLEPSAANWVMSGEVL
                     AFMVPEMSLGSPHLPEKICAKDYVDALDGLVLQGGADIAPQTYGETPLDPRWSGDPIR
                     DRYEISLFEEFVRQGKPVLGICRGCQLINVALGGTLYQDIGTQVGKSIVHRSDTRYED
                     NFHAMRLIEGGWLAGLYPRAGISRINTIHHQAVKGLGRDLVVEALSEPDGVVEALRWK
                     GPGFVVGVQWHPEFMDPADAQLLDGRPLLNAFITACRSRAN"
     gene            4439..5119
                     /locus_tag="VFV55_08445"
     CDS             4439..5119
                     /locus_tag="VFV55_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX4944369.1"
                     /translation="METELATIARTALVTLAALLPITNPPGNAPIFLALTGGMAEEAR
                     RAMARRVGLNCLVLLVAAAFVGTHVLAFFDISLPVVRVGGGLLVAATAWRLLAAEGGA
                     SDDSLGATRLTAAHEVAGRAFYPLSFPITVGPGSISIAITLGASLPKRGVPLLTTSAG
                     LLIGITVAAAAIWLAYRYASHLVRLLGATGTAVFLRLSAFILLCIGVQICWDGLAELL
                     APWRPQSR"
     gene            5130..5870
                     /locus_tag="VFV55_08450"
     CDS             5130..5870
                     /locus_tag="VFV55_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4944370.1"
                     /translation="MTAATTGRVAVITGGAGALGFATARALLEDGTRVALVDVDALRL
                     DTLSRFVRGTCIVIAADVTDVSAVRAAHDRVRTELGPVDILVNAAGASSDAALAATDE
                     TAWRRILGARLDGTFQWSRAVLPEMTARGWGRIVNVGGNYQPSLLRGPAEAAASGAVA
                     SLSAALAREAAPGGVTVNAIAPAFVRSPAATEHLSEAQRRQALASIPAGRFGELEEFA
                     HAVRFLASPLAGFITGEILNLDGGLHLG"
     gene            5965..6537
                     /locus_tag="VFV55_08455"
     CDS             5965..6537
                     /locus_tag="VFV55_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX4944371.1"
                     /translation="MPVPQTEPTGAPSPREALAQCHLCIAAMLTRLGSIEGELVRTLP
                     GTPAPDLPDRVRALVAEIDEALSVHIADEEADVFPALLAAAESPARRAQAFELVSSLL
                     VEHREIAELWHALRIALLAAGGGVYIAYPGGTAADFLLRVLGHLERERTELAELMEVL
                     DTAKSRAIAISIAHRHDESCPRIRNCPLKP"
     gene            6834..7985
                     /locus_tag="VFV55_08460"
     CDS             6834..7985
                     /locus_tag="VFV55_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX4944372.1"
                     /translation="MRRSNRPMLTGQEKADLWRRWQSGESVADICRALNKYEGSIHRV
                     LAARGGIVPATRRRSPMSLSLSEREHISRGIAANSSIRQIASHIGRAPSTVCREIARH
                     GGRARYRASEADLQAWDSARRPKPCRLASRPRLRWEVARKLRRNWSPEQISGWLKGEY
                     PDDEDMHVSHEAIYQSLFIQARGVLKKELMGHLREVRTMRRSRDRGAYVENRGKIVDA
                     VSIRERPAEVEDRAVPGHWEGDLLSGSKNTHIATLVERQTRFTMLVKVRGRDTQSVVS
                     AISRQVRKLPAELRRSLTWDRGKEMTDHKQFTIATDLKVYFCDPQSPWQRGTNENTNR
                     LLRQYFPKGTELSSHTQAHLNRIALELNQRPRKTLGFKAPADKLNEVLL"
     gene            complement(8102..>8819)
                     /locus_tag="VFV55_08465"
     CDS             complement(8102..>8819)
                     /locus_tag="VFV55_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4944373.1"
                     /translation="VQTDIYSLGVVMYELLTGRLPFRAENAVAMINKILNEDPLPIEE
                     LRPDLPEKLVEIVQKAMHKDPKVRYGTWFEMASELANTFPQLERYSHEISSTEKFNKL
                     RSLPFFREFRDAELWEVLRGAVWESHGRDQNLLLEGEIGQAFFIIVGGQVKVVKDGKL
                     LNVLKEGDCFGEMAYLSGDRARRSASITSVSEVHLLKIQAAHLESLTDGCQLRFTREF
                     LRTLIERLTWTSAVLAQFRR"
BASE COUNT         1344 a         3099 c         2987 g         1389 t
ORIGIN      
        1 gggcatcggc gcgccagcgc gccacttccg agagcagacc ctcggggcgc tcgaccacca
       61 cctccggctc gccttcgagc acgcggccgc gctccgcgtc gaggcagatc gtctcgccct
      121 cgcgcaaagt gcgcgtcccg aggacgatgg cgcgggcctt ttcgtcgatg cgcagggtgg
      181 cgcagcccac gaggcagacc ttgcccatct gccgcgcgac cacggcggcg tgcgaggtgc
      241 gcccgccgcg cacggtgaga acgccggcag cgatggcgat gccggcgatg tcttcggtgc
      301 tggtgtcgct gcgcacgagg atcgccggcc ggccccgcgc ggccagccgt tcgcaggcct
      361 tcgcgtcgag cgcgatcgcg cccacggcga gcccgatgct cgcgggttcg gcgaccgcca
      421 gtgccgcctc gccttcgtcc gcgacgcggc ggcgctcgat cgcggcgagg tcgatgccgt
      481 cgaggcgcgc gagcgcctgc gccggcgtgg ccaggccctc gcgcacctgc tccaccgcca
      541 tgcgcagcgc cgcccacggc gtgcgcttcg ccgcgcgcga ctgcagcagg tacaggcgcc
      601 cgtcctggac cgtgaactcg aactcctgcg cgtcgccgaa agtcgtctcc agcgcgtggc
      661 gcagctcgtc cagccgctgc gcgacttccg gcagtgcgac gggaagcgcg gcagtatcgg
      721 tgacggcgtg gcgcccggaa accacgtcct cgccctgcgc gttgaacagg aaatcgaggt
      781 aaaggcggtt ttctcccgtc gaagggtcgc gcgtgaagcc cacgccggaa ccggaagtgc
      841 cgccggcgtt gccgaacacc atgcgctgca ccgtgactgc ggtgccgatc gcatcgtcga
      901 tcgcgtgcag ccgccggtat tcctctgcgc gcgccgaatg ccacgagtcg agcaccgcga
      961 cggtggccgc ctcgagctgc tcgcgcgggt cctgcgggaa cggctcgccc gtgatgtccg
     1021 cgaagcgctc gaggctcccg gcattgagca ttaccaggct ctcgaagtcg agttcgcgag
     1081 cgccggccac gcccgcgctc gacaccgcgg cagcggcgag ttcgtcgaac gccgcgccat
     1141 cggcaccgtg cacgacctgc gcgtattgct ggacgaggcg ccggtaggaa tcccacacca
     1201 ggcgcgggtt gcccgtggag cgcagcagcc cgcgcacggt gcgatcgcaa agcccgatgt
     1261 tgagcacggt gtccatcatc cccggcatcg acaccggcgc acccgagcgc acggagacca
     1321 atagcggcct gcgctcgccg ccgaaggaaa gcccgcaggc ctgctcgagg cgccgcgtcc
     1381 actcgacgag cgccacgcgc agcggctgcc gcgcggcggc ggggtcgcgc tggtgcaggc
     1441 ggcaccagtc cgtaccgagc acgaaggcct gcggcaccgg catgcccgcg gccgccattc
     1501 gctgcaggtt gaaggccttg aagcccatgc cggtgacatc gccgggcacg gcacccgcgt
     1561 gcggaaggcc gatcccgaag acggcgcctt cgccggggct cactggacca ccagctcgcc
     1621 gagcacggcg tcgcgcaatt tcagtgccgt gtgcatgagc gcgtccgtgg cgccctcgag
     1681 gttgcgcgcg cactcgatga acccgaagag gcccgcgaat tcgcccgcct gcgccggcac
     1741 gaggctcttg gccgagcggt gcacctcgtc gccgcgccgc tcgagcgtca tgatgcggtg
     1801 gacggcctcg aggaaatcct gcgtgtcctc gcgcgggctg ccgcgatgca gggtgcgcgc
     1861 cgtctcgacg gccttgaggt attcctgcac gctctccacc gcgagcgcgg caagggggcg
     1921 cagcgccgcg tacagcgcct cgccgcggcc gtcgtggggc atgagcgcga ggtggaacgc
     1981 ggcttcctcg agctcgtcga cgatgtcgtc ggcgacctcg atcaggtcgc ggtaggtgcg
     2041 cgcgcgttcc gagtggcgcg cctgggcgcg ggccgcgttg acgagctcgt cggcgcggtg
     2101 ctcccacgcc ttggcccgcc gggcgttgcg ggcgaacgcc tcgccggccg agggcgcgcc
     2161 ggcactgagg agcgcgtcgc ggatgccgct cgcgatctcg agggcgaagg cggcgtgctc
     2221 ggccgcgacg tcgagcacgc tttcctgcgc cgagcggaag tagctcacca gttccgcgcg
     2281 cacctcgtcg cgcaccagcc cctcgggccg ccctccctgc agcccctggg cgcaggtgcg
     2341 catgacgtag cgcatgaact tcaccgcggc gtcgtggccg agcacgtcgt cgaggcgcgc
     2401 gccgaaggac gacggcgccc gcgacacgaa ggcgagcgaa tcgaagatca tctgctcgcc
     2461 gcccgcgcgc aggaacgcca tgtggccgtg gtcgttgtcg gccgcccact tcagcagctc
     2521 ggccacctcc ttcctgggca gcagcaggcg caggcgcttg cgtgcgcggt tccagtcgat
     2581 gaggaacacc agccgcgagc ccatgaacgc caggtagtcc tccagctcgg cgtcgctcgc
     2641 cgccgtgaac gagccgacgc acaggtgata gacgccatcg gccatgctcc ggtccgtgcg
     2701 cgacagcgtg tccgcccatt cgacccgccg ggcgtcgaag aggccctgga agaagaccag
     2761 ccgctgcatg tgcacgtcgg tataggtgag cgtcgcgcgg ttcgcctcca catggaccac
     2821 gaggacatgg gcatccgtgg tgccgatgtc gttctgcagc accaggcgat cgcccgatcg
     2881 cgtggccgtg gtgccgagcc ccgggtggtc gaacttgagc gcggcggtgc ggttcacccc
     2941 gcgcatgaag gccgccacca gcggccggtc gcccggcgcg atgccgtagg cgtgggcacc
     3001 ctcgatgatc tccgaggaga tgcgcgcctg caggccgttg agggccttgt gcatgtccat
     3061 caccaggcga tgcacgctgt cggttccctc gtgcccgccc gaggcgatcg cgccgatctg
     3121 gtcgccggtg atctggtcgt ccgcgccgca ccagggcacc tcgtccagcc gccgacggcg
     3181 ttcgtcgtag gcggcggcat cctcgcccga ggcaagcaac ggggcaagca tggcgtcgat
     3241 ctcggcgcgc agccgggtgc acaggcgctc gaccccgggc atgtagtagc ggccgtctcc
     3301 cgtcttcgtg gcggcggccg cgacctcgtc gaagcccgcc gccgccagcc ccgcggcctc
     3361 gcgttcggcg cgcaactccg gcgcggcgct cccggggcgc tcggcgtggc cctgcgcgac
     3421 ctgcaggacg gtgaacaggt acttcgcccg gtcgttggcg gccagcgcct cgttgagcag
     3481 cgcgggcagc ctcaggccct cttcacccag tgcttcgacg atgcgcgatt tttccatggc
     3541 gggaacggcg gctacgcctc caatctagcc gggatgcgcg gcgcgttcct gtcttaggtc
     3601 aacaggtcgc cacgcggcgc gccggccgcc gacgggatat agtttgccga tggctcccct
     3661 gaagataggc ctctcggcgc ggctcctcta cccggacccg cgacgcacct ttcttccgac
     3721 aaagtgcatc cagtatctcg agccctcggc ggcaaactgg gtgatgtcgg gcgaagtgct
     3781 ggccttcatg gttcccgaaa tgagcctcgg ttccccgcac cttccggaga agatctgtgc
     3841 gaaggattat gtcgatgcgc tcgacggcct ggtgctgcag ggcggcgccg acatcgcgcc
     3901 gcaaacctac ggcgagacgc cgctcgaccc gcgctggtcc ggcgatccga tccgcgaccg
     3961 ctacgagatc tccctgttcg aggaattcgt gcgccagggc aagcccgtgc tcggcatctg
     4021 ccgcggctgc cagctcatca acgtggccct gggcggcacc ctctaccagg acatcggcac
     4081 gcaggtgggc aagtcgatcg tgcaccgcag cgacacgcgc tacgaggaca acttccacgc
     4141 catgcgcctc atcgagggcg gctggctcgc cggcctttat ccgcgcgcgg ggatctcgcg
     4201 catcaacacc atccaccacc aggccgtgaa gggcctcggc cgggacctgg tcgtcgaggc
     4261 gttgagcgaa cccgacggcg tcgtcgaggc gctgcgctgg aaggggccgg gcttcgtggt
     4321 gggcgtgcag tggcacccgg agttcatgga ccccgccgat gcgcaactcc tcgacggcag
     4381 gccgctgctc aacgccttca tcaccgcgtg ccgcagccgc gcgaactgac gacacgcctt
     4441 ggagaccgag ctcgccacca ttgcccggac cgcgctggtg acgctggccg cgctactccc
     4501 catcaccaac ccgccgggaa atgcgccgat cttcctcgcg ctcaccggcg gcatggccga
     4561 ggaagcccgg cgcgccatgg cgcgccgcgt cgggctcaac tgcctcgtgc tcctcgtggc
     4621 ggcggcattc gtcggcacgc acgtgctcgc gttcttcgac atttcgctgc cggtggtgcg
     4681 cgtgggaggc gggctcctcg tcgcggccac ggcctggcgg ttgctcgccg ccgagggcgg
     4741 cgcgagcgac gattccctcg gcgccacgcg gctcaccgcg gcgcacgaag tagccgggcg
     4801 ggccttctac cccctttcgt tccccatcac ggtggggccg ggctccatct ccatagccat
     4861 cacgctgggc gcgagcctgc ccaagcgggg ggtgccgctg ctcacgacct ccgccgggct
     4921 tctcatcggg atcacggtcg ccgccgcggc catctggctc gcctaccgct acgcctcgca
     4981 cctcgtgcgg ctgctgggtg ccacgggcac ggccgtgttc ctgcggctct cggcgttcat
     5041 ccttctctgc atcggcgtgc agatctgctg ggacgggctg gcggaactcc tcgcgccgtg
     5101 gcgcccgcaa tcgcgataag gagacgaacg tgaccgcggc gacgacaggc agggtggcgg
     5161 tgataacggg cggcgcgggg gcactcgggt tcgcaaccgc gcgcgccctg ctcgaggacg
     5221 gcacgcgcgt ggccctcgtg gacgtcgacg ccctgcggct cgacacgctg tcgcggttcg
     5281 tgcgcggcac ctgcatcgtc atcgccgccg acgtcacgga cgtctccgcg gttcgcgcgg
     5341 cccacgaccg cgtgcgtacc gagctggggc ccgtggacat cctggtgaac gcggccggcg
     5401 cctcctcgga cgcggccctc gccgccaccg acgagacggc ctggcggcgc atcctcggcg
     5461 cgcgactcga tggcaccttc cagtggtcgc gggccgtgct gcccgaaatg accgcgcgcg
     5521 gctggggccg catcgtcaac gtgggtggca actaccagcc gtcgctcctg cgcgggccgg
     5581 ccgaggccgc ggcctcgggc gccgtggcct ccctttccgc ggccctcgcg cgcgaggcgg
     5641 cgcccggcgg cgtcacggtg aacgccatcg ccccggcctt cgttcgctcg cctgccgcca
     5701 cggagcacct ctccgaagcg caacggcgcc aggcgcttgc ctcgattccc gcaggacgct
     5761 tcggcgagct cgaggagttc gcccacgccg tgcgcttcct tgcttccccg ctggccggct
     5821 tcatcaccgg agaaatcctg aacctcgatg gcgggctgca cctcggctga tcgcgcgcgg
     5881 ccggcgctgt tcggttttct tgatttcctt taatccttcg gcccacgcgc cccggtagtc
     5941 tggccccttg attcaggagg gccgatgcca gttccgcaga ccgaaccgac cggcgccccg
     6001 tccccgcggg aggcgctcgc gcaatgccac ctgtgcatcg ccgcgatgct cacccgcctg
     6061 ggctcgatcg agggcgaact ggtccgcacg cttccgggca cgccggcgcc cgacctgccg
     6121 gaccgtgtcc gcgcgctggt cgcggagatc gacgaggcgc tcagcgtgca cattgccgac
     6181 gaggaggccg acgtgtttcc cgcgttgctg gccgcagccg aatccccggc tcgccgcgcc
     6241 caggctttcg agctggtgtc ctcgctgctg gtggagcacc gggagatcgc cgagctgtgg
     6301 cacgccctgc gcatcgcgct gctcgcggcc ggcggcggcg tctacatcgc ctacccgggc
     6361 ggcaccgcgg cggactttct cctgcgcgtg cttggccacc tcgaacgcga gcgcaccgag
     6421 ctcgccgaac tgatggaggt gctcgatacc gcgaaatcgc gggccatcgc catttccatc
     6481 gcgcaccgcc acgacgagtc ctgcccgcgc atcaggaact gcccgctgaa accctgaccc
     6541 gccgccaggg tcggctccga ccatgaccac agcgtccatt gctggaggcg accttcgagt
     6601 cagaaagccg ctgtctccgt gatggccgat gtcgaaacga gaggcgtgtt cggggtcagg
     6661 ttggatgtgt tttttttggt tttcggccag gtcaaattgc gtcggacgtc acggttcgaa
     6721 cctatttcga tgtgctgccg gagtcgaaac tgacagcaag ttcatctggg gcggattcaa
     6781 tgggtcgatg caacaccccg ttattctgaa tcactttcaa gggggtggtg catatgaggc
     6841 gaagcaatcg gccgatgttg acgggccagg agaaggcaga tctttggcgc cggtggcaat
     6901 ccggggagtc ggtggctgat atctgtcgtg cgctgaacaa gtacgagggt tcgatacacc
     6961 gcgtattggc ggctcggggt gggattgtgc ctgcgacccg tcggcgctcg cctatgtcgc
     7021 tgagcttgag tgaacgcgaa catatctctc gcggaatagc tgccaatagc tccattcgcc
     7081 agatcgcgag ccacatcggg cgagcgcctt caacggtgtg tcgggaaatt gcgcgccatg
     7141 gagggcgagc gcggtatcga gcctcggagg cggacttgca ggcgtgggac tcggccaggc
     7201 gccccaagcc atgtcgcttg gcgagccgtc cgcggctacg atgggaagtg gccaggaagc
     7261 tcaggcgcaa ctggtcacct gaacagatat ccggatggct caagggtgaa taccctgatg
     7321 atgaagacat gcacgtgtcc cacgaagcga tctaccagag tctgtttatc caggcccgag
     7381 gggtgctcaa gaaggaactg atggggcacc tgcgagaggt gcgcaccatg cgccgctccc
     7441 gggaccgcgg agcctatgtc gagaaccggg gcaagatcgt cgatgccgtc tcgatcaggg
     7501 agcgtcccgc cgaggtggag gaccgggccg ttccgggcca ttgggagggg gacctgctta
     7561 gcggctcgaa gaatactcac attgccactc tcgtggagcg ccagacccgg ttcacgatgc
     7621 tcgtgaaggt tcgaggaaga gatacccaaa gcgtcgtctc ggcgataagc cggcaagtgc
     7681 gcaagcttcc ggctgaactg cggcgatcgc tgacgtggga tcgaggcaaa gagatgaccg
     7741 accacaagca attcacgata gccacggacc tgaaggtcta cttctgcgat ccacaaagtc
     7801 cctggcagcg cggcacaaac gagaacacca atcgcctgct gcgacagtac ttcccgaagg
     7861 ggaccgaact atccagccat acccaggccc atctgaacag gattgcgcta gaactgaacc
     7921 agcgcccgcg aaagacactc ggcttcaagg ctcccgcaga taaactaaac gaagtgttgc
     7981 tttgaccgat tgaatccgcc ggccaaaaac cgaaaaaaaa caccaaccac accctgctcc
     8041 ttcaccgcga tgctgcaagc gccatcgcta cgtgcccgga gccaccacga ctcccgcgcg
     8101 cctagcggcg gaactgcgcg agcaccgcgg aggtccaggt gagccgctcg atgagcgtgc
     8161 gcaggaactc gcgcgtgaac ctgagctggc agccatcggt gaggctctcc aggtgcgccg
     8221 cctggatctt gagcaggtgc acctcggata cggaggtgat cgatgccgag cggcgcgcgc
     8281 ggtccccgga gaggtaggcc atctcgccga agcagtcgcc ttccttgagg acgttgagca
     8341 gcttgccgtc cttgaccacc ttcacctgcc cgccgacgat gatgaagaac gcctggccga
     8401 tctcgccctc gagcagcagg ttctggtcgc gcccgtggct ctcccacacg gccccgcgca
     8461 ggacctccca gagctcggcg tcgcggaact cgcggaagaa gggcaggctg cgcagcttgt
     8521 tgaacttctc ggtcgagctg atctcgtgcg aatagcgctc gagctgcggg aaggtgttgg
     8581 cgagctcgct cgccatctcg aaccaggtcc cgtagcgcac cttcgggtcc ttgtgcatcg
     8641 ccttctgcac gatctcgacc agcttctcgg gcaggtcggg gcgcagctcc tcgatgggca
     8701 gcgggtcctc gttcaggatc ttgttgatca tcgccaccgc gttctcggcg cggaagggga
     8761 gccgccccgt gaggagctcg tacatcacga cgccgagcga atagatgtcc gtctgcacg
//
LOCUS       DASUEV010000262         8411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1570976, whole genome shotgun sequence.
ACCESSION   DASUEV010000262 DASUEV010000000
VERSION     DASUEV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570976"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1109)
                     /gene="purM"
                     /locus_tag="VFV55_08470"
     CDS             complement(69..1109)
                     /gene="purM"
                     /locus_tag="VFV55_08470"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX4944374.1"
                     /translation="MTAAPNPLTYRDAGVDIDAGDELVERIKPYARRTMRPEVLSGLG
                     GFGALVEVSKKFRNPVMVSGTNGVGTKLKLAFRMKKHDTIGIDLVAMSVNDILVTGAE
                     PIFFLDYFACGKLRVDVAASVVKGVAEGCEQAGCALIGGETAEMPGLYDPNEYDLAGF
                     AVGLVEKERIIDGSTIVPGDTVVGLASSGAHSNGYSLVRKILAVTGADLDESFDGRTL
                     GETLLEPTRIYVKPVLALMGKIPLKGIAHITGGGLVENVPRVLPANTAARLEKSRWPR
                     PAVFDWLQEQGNVPEPEMHRTFNCGIGMVLVMGREHAQAAIAALGIHGIPAWEVGTIV
                     ARAGDGPQAAVV"
     gene            1241..1531
                     /locus_tag="VFV55_08475"
     CDS             1241..1531
                     /locus_tag="VFV55_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944375.1"
                     /translation="MNGMTRTFALASITAAALALPAAHASEALDRDIFVTVLDARKDE
                     MVTKAEAMKQAEKMVGEQVEKFFEKQGAKNDGKVARSQLDRFLRELMNYGGA"
     gene            1626..2234
                     /locus_tag="VFV55_08480"
     CDS             1626..2234
                     /locus_tag="VFV55_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4944376.1"
                     /translation="MAQQATRDPRYTEIESHRPYLVRYALAQLRDAQLAEEAVQEALL
                     AALEGLSGFNGQSSLRTWLTSILRFKVIDLQRRAATDRQREAHTDYAEDAEAGDETWL
                     DDLFDETGHWREAPQTWSNPESALEQRRFWEVMEKCLDRLPAAGGRVFFKREVLGEDT
                     PAICQSEGITESNCWVILHRARIALRGCLEANWFGQETARGR"
     gene            2224..2409
                     /locus_tag="VFV55_08485"
     CDS             2224..2409
                     /locus_tag="VFV55_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944377.1"
                     /translation="MADSGKVDCRKASWLMSVARERELSAEESEALRLHLGRCLKCRN
                     FEEQLRFLHEAARRFGK"
     gene            complement(2476..3162)
                     /locus_tag="VFV55_08490"
     CDS             complement(2476..3162)
                     /locus_tag="VFV55_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX4944378.1"
                     /translation="MSRSLAGLLLAGVSVLSASAAEPAPGKPVYGEGRMLVLVSPQFP
                     AAALAKGETGKVDVFGTIRTDGSLENVRIETSPPNESFESAVMNVVPLWRLQPRIVTP
                     DCGAASTKGQVTIWFELDGGKPKVSYGTRPPPGTAPPAIHIDRRPIRTVTPNYPAKLA
                     QDPKAPKSMVQVAYVAVAEDGAVTGVTLAPMLYYRDFEPNIVAAMRQWKFEQQVQAWC
                     GEFEMVLAMQ"
     gene            complement(3195..4280)
                     /gene="nadA"
                     /locus_tag="VFV55_08495"
     CDS             complement(3195..4280)
                     /gene="nadA"
                     /locus_tag="VFV55_08495"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX4944379.1"
                     /translation="MAVATPIQIRFERFDGALGIAPATPPHVVAPEEKPALIRRIKER
                     LKALDAVFVAHYYVHPDLQDLAQETGGTVSDSLEMARFGHDHPGRTIVVAGVRFMGET
                     AKILNPEKRVLMPDLAAECSLDLACPAGEFTAFCDAHPDRVVVVYANTSAAVKARADW
                     MVTSSIAEKIVGHLHAQGKKILFAPDKHLGAYIRKVTGADMLLWNASCVVHEEFKAQQ
                     LEEMKLKHPGAKVLVHPESPAGVIAMADAVGSTTGIIQAAQRLGAKEYIVATDGGIIH
                     QMRKRLPGVTFIEAPTAGKGATCQSCAHCPWMAMNGLHNLLEVLETGKNEIHVDPALG
                     KRAKVPIQRMLDFAKEHLPAMKGSGDA"
     gene            4457..5140
                     /gene="hda"
                     /locus_tag="VFV55_08500"
     CDS             4457..5140
                     /gene="hda"
                     /locus_tag="VFV55_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX4944380.1"
                     /translation="MRQLLLDFTQAPAPTFENFVPGRNAEPLAALRAALEERGRFPFP
                     ERAKSEASPVLYLWGETGSGKTHLLQAFADGRDGARFVRGEDYLGTESGGVLVLDDVE
                     RLGEARQVALFNAFNERAFALIVVSAHAAPRDVALRRDLATRLATGFTYRLLPLTDEE
                     KSQALAAHARARGFALSDDVAAYLLTHARRDMPSLIRALDALDRYSLETGRPITVPLL
                     KAALQPELT"
     gene            5137..5799
                     /locus_tag="VFV55_08505"
     CDS             5137..5799
                     /locus_tag="VFV55_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX4944381.1"
                     /translation="MKLALFDLDNTLLDGDSDYCWAQYLIERGILDREGYEKKNDWFY
                     HHYKQGTLDIHDYLDFQLAPIAGRPREVIDEWHREFMERKIRPIIHAGTPALIASHGD
                     ALKAVVTATNRFITEPIARELGVEHLIACDVELMPDGRLTGRPTGTPSFREGKVARLH
                     EWLATLGRRFEDFDETWFYSDSHNDLPLLEMVSKPVAVDPDPTLESHARERGWPILRL
                     RA"
     gene            5796..7187
                     /gene="pcnB"
                     /locus_tag="VFV55_08510"
     CDS             5796..7187
                     /gene="pcnB"
                     /locus_tag="VFV55_08510"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX4944382.1"
                     /translation="MIKSFISRVFGRGAKQAAHPSKPVIYGPDKHHIRKDHISRGARK
                     TCEELQRAGHTAFVVGGAVRDLILGMQPKDFDVATSALPDEVRSIFRRSRIIGRRFQI
                     VHVMWGAETVEVSTYRAHFTTKDPEAEVEIDKQDEHGRVLSDNVFGTQAEDAVRRDFT
                     VNALFYDPVKEEVWDYQHGVRDLVARKLVTIGDPTTRFREDPVRMLRAARFSAKLGLA
                     IDPSTAAPIASLKHLLENVPQARLFEEILKMLLSGNAVACVQRLRDLDLHHGLLPLLD
                     DALDDPATGPFALAALKATDDRLRGDKPVSPAFLLAALLWGRVERRWHELEEKGQPST
                     PSLHSAMHDALDAQRESLAIPRRFDATMKELWLLQPRFMQRGGTRPFRLVEHPRFRAA
                     YDFFELRAQSGNAPLEAAEWWARFQKASGDERSRMLHADEGPKKKKRRRRGGKRHPED
                     GGSPEEVAPGSDE"
     gene            7184..7678
                     /gene="folK"
                     /locus_tag="VFV55_08515"
     CDS             7184..7678
                     /gene="folK"
                     /locus_tag="VFV55_08515"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX4944383.1"
                     /translation="MNAVRAVVALGSNLDGPEARVRLAFDEVGVLPSTRLLARSALYR
                     TAPVGYLDQPAFVNACALVETSLGPRELLEGLLAIERRHGRVRDIPDGPRTLDLDIAL
                     YDDLVMHEHGLTLPHPRAHERAFVLVPLLDVWPDAVIPGRGAAASLRDRVGDQAIERL
                     PDNP"
     gene            7675..8304
                     /locus_tag="VFV55_08520"
     CDS             7675..8304
                     /locus_tag="VFV55_08520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX4944384.1"
                     /translation="MRHHFIVVEGPIGCGKTSLAKLLARRLNAGLILEEPEANPFLPL
                     FYRDMRRHALSTQLFFLFQRLHQLEGLRQPDLFGKPTVADFALQKDPLFARLTLDDNE
                     FGLYTRIFDHVRPQAPTPDLVVYLQAKVETLVERVRRRGNPMEAGISDEYLRAISDTY
                     TRYFYNYGESALLIVNSERLNFVDVPEHFDMLVERVKSIRGGREFFNRA"
BASE COUNT         1324 a         2895 c         2812 g         1380 t
ORIGIN      
        1 caaaaaacag gcaatggaac ccggtcccat tgcctgtttt ttgtgcaaag taacctgacc
       61 ccttttcctc aaacgaccgc ggcctgcggt ccgtcgccgg cgcgcgcgac gatggtgccg
      121 acctcccacg ccgggatgcc gtggatgccg agcgcggcga tggcggcctg cgcgtgttcc
      181 ctgcccatga cgagcaccat gccgatgccg cagttgaagg tgcggtgcat ttccggttcg
      241 ggtacgttgc cctgctcctg cagccagtcg aagacggccg gccgcggcca gcgcgacttc
      301 tcgaggcgcg cggcggtgtt cgccggcagc acgcgcggca cgttctccac gagcccgccg
      361 ccagtgatgt gcgcgatgcc cttgaggggg atcttcccca tcagcgcgag aaccggcttc
      421 acgtagatgc gcgtgggttc gaggagcgtc tcgcccaggg tgcggccgtc gaaggactcg
      481 tccaggtcgg cgccggtgac cgcgagaatc ttgcgaacca gcgagtagcc gttggaatgc
      541 gcgccgctgg aggcgagccc gaccacggtg tcgccgggga cgatcgtgga cccgtcgatg
      601 atgcgttcct tctccaccag cccgaccgcg aagcccgcca ggtcgtattc gttcgggtcg
      661 tagagccccg gcatctcggc ggtctcgccc ccgatgagcg cgcagccggc ctgttcgcag
      721 ccctcggcca cgcccttgac gacgctggcc gccacgtcca cgcgcagctt gccgcaggcg
      781 aagtagtcga ggaagaagat cggctcggcg ccggtgacga ggatgtcgtt cacgctcatc
      841 gccacgaggt cgatgccgat ggtgtcgtgc ttcttcatgc ggaaggcgag cttcagcttg
      901 gtgcccacgc cgttcgtgcc ggagaccatt accgggttcc tgaacttctt cgacacttcc
      961 accagcgcgc cgaatccgcc gaggccgctg aggacctccg ggcgcatcgt gcgcctggcg
     1021 tagggcttga tgcgttcgac cagttcatcg cccgcgtcga tgtcgacgcc ggcgtcgcgg
     1081 taagtgaggg gattcggtgc ggcggtcacg gcggcgcttc cggagtcgga acagactgaa
     1141 attctaccgc aacgccccct tcgcgtcgcc tcgtaaggat tggcggatcg gctcgacata
     1201 ccgttcgcat gacccaatcc aaccctcaag gagaatcgac atgaacggca tgacacgcac
     1261 tttcgccctt gcctcgatca ccgccgctgc gctcgcgttg cccgccgccc acgccagcga
     1321 agcgctcgac cgcgacattt tcgtcaccgt gctggacgcc aggaaggacg aaatggtcac
     1381 gaaggccgag gccatgaagc aggcggagaa gatggtcggg gagcaggtgg agaagttctt
     1441 cgagaagcag ggcgcgaaga atgacggcaa ggttgccagg agccagctcg accggttcct
     1501 cagggagttg atgaactacg gcggtgcctg aggccgcggt catccggccc ggcggccggg
     1561 gcgcggggtc cgcctgccgc gcggtccccg tatgttccgc gggtggtggt aagctttgcg
     1621 ccaccatggc gcagcaagcc acccgcgacc cccgctacac ggaaatcgag agccacaggc
     1681 cctatctcgt gcgctacgcg ctcgcccagc tccgcgacgc ccagcttgcg gaagaggccg
     1741 tgcaggaggc gctgctcgcc gcgctcgagg gcctgtccgg gttcaacggc cagtcgagcc
     1801 ttcgcacctg gctcacctcg atcctgcgct tcaaggtgat cgacctgcag cgccgtgccg
     1861 ccaccgatcg ccagcgcgag gcgcacaccg actacgcgga agacgccgag gcgggcgacg
     1921 agacctggct cgacgacctc ttcgacgaga ccggccactg gcgcgaggcg ccgcagacgt
     1981 ggagcaatcc cgaatcggcg ctcgagcagc ggcgcttctg ggaggtcatg gaaaaatgcc
     2041 tcgaccggct gccggccgcg ggcggccgcg tcttcttcaa gcgcgaggtt cttggcgagg
     2101 acacgccggc gatctgccag tccgagggca tcaccgaatc gaactgctgg gtgatcctgc
     2161 accgcgcgcg catcgccctt cgcggctgcc tcgaggccaa ctggttcggg caggagacgg
     2221 cgcgtggccg atagcgggaa ggtcgactgc cgcaaggcct cctggctgat gtcggtcgcc
     2281 cgcgagcgcg agctgagcgc cgaggaatcc gaagcgcttc gccttcacct cggacgctgc
     2341 ctgaagtgcc gcaatttcga ggagcagctc aggttcctcc acgaggccgc gcgccgcttc
     2401 ggaaagtgat ctccgggtgt ccggtaccgt cgctgcgcgc cccatcgcgc ccgcgccgca
     2461 gcgcggcccg gggggtcact gcatggcgag gaccatctcg aattcgccgc accacgcctg
     2521 gacctgctgc tcgaacttcc attgccgcat cgccgcgacg atgttgggct cgaagtcgcg
     2581 gtaatagagc atgggggcga gggtcactcc ggtgacggcg ccatcctcgg ccacggccac
     2641 gtaggcgact tgcaccatcg acttcggcgc cttcgggtct tgcgcgagct tcgcgggata
     2701 gttcggcgtg accgtgcgga tgggtctgcg gtcgatgtgg attgccggtg gcgcggtccc
     2761 cggcggcggg cgcgttccgt agctcacctt cggcttgccg ccgtccaact cgaaccagat
     2821 cgtgacctga cccttcgtgc tggcggcgcc gcaatccggc gtgacgatgc gcggctgcag
     2881 ccgccagaga ggcaccacat tcatgacggc cgactcgaac gactcgttgg gcgggctcgt
     2941 ctcgattcgc acgttctcca ggctaccgtc ggtgcggatc gtcccgaaga catccacttt
     3001 gccggtctcg cccttcgcca gcgccgccgc tggaaattgt ggcgatacca ggacgagcat
     3061 ccggccttcg ccataaacgg gcttgcctgg cgccggttcg gcggcggatg cggacaagac
     3121 ggagactccc gccaggagca ggccggccag cgatcgcgac atgggtattc ctcgggttga
     3181 cgggattgcg gtgctcaggc gtcgccgctg cccttcatcg cgggcaggtg ttcctttgcg
     3241 aaatcgagca tgcgctggat cgggaccttg gcgcgcttgc ccagggccgg gtcgacgtgg
     3301 atttcgttct ttccggtttc cagcacttcc aggagattgt gcaggccgtt catggccatc
     3361 cacgggcaat gcgcgcagct ctggcaggtg gcgcccttgc cggcggtggg tgcctcgatg
     3421 aaggtcacgc cgggaaggcg cttgcgcatc tggtggatga tgccgccgtc ggtggcgacg
     3481 atgtattcct tcgcgccgag gcgttgcgcg gcctggatga tgccggtggt ggagccgacc
     3541 gcgtcggcca tcgcgatgac gcccgccggc gattccgggt gcacgagcac cttggcaccc
     3601 gggtgcttca gcttcatctc ctcgagctgc tgcgccttga attcctcgtg cacgacgcag
     3661 gaggcgttcc acaggagcat gtccgcgccg gtcaccttgc ggatgtaggc ccccaggtgc
     3721 ttgtccgggg cgaagaggat cttctttccc tgcgcatgca ggtggccgac gatcttctcg
     3781 gcgatggagg aagtgaccat ccagtcggcg cgagccttca ccgcggcgct ggtgttggcg
     3841 tagaccacga cgaccctgtc gggatgcgcg tcgcagaatg cagtgaactc gccggcggga
     3901 caggcgaggt cgagcgagca ctccgcggca aggtccggca tgagcacgcg cttctcgggg
     3961 ttgagtatct tggccgtctc gcccatgaag cgcacgcccg ccacgacgat cgtcctgccg
     4021 gggtggtcgt ggccgaagcg cgccatttcc agggagtccg acaccgtgcc gcccgtctcc
     4081 tgcgctaggt cctgcaggtc cggatgcacg tagtagtgcg ccacgaacac cgcatcgagc
     4141 gccttcaggc gttccttgat gcgccggatc agggccggtt tttcctccgg cgcgacgacg
     4201 tgcggcggcg tggccggtgc gatgcccagt gcgccatcga agcgctcgaa ccggatctgg
     4261 atgggggtgg cgacggccat ggggatttcc tctgcgtgtt cgattctgcc acaggggaat
     4321 cgttcatgcc ccgagggggc gcgtgctaga atccgcctcg tttccctcag cgttccgcct
     4381 gcgtttccgg cgggtcgcgc ccgggccaag tcgggcggcg caatcctgcg ccgcggcgca
     4441 ggcccaggga gtcccgatga ggcagcttct cctcgacttt acccaggccc ccgcgcccac
     4501 cttcgagaac ttcgttcccg gccgcaacgc cgagccgctg gccgccttgc gtgccgcgct
     4561 ggaagagagg ggacgcttcc cttttccgga acgggcgaaa agcgaagcgt cccccgtgct
     4621 ctacctctgg ggcgagaccg gctccggcaa gacgcacctg ctgcaagcct tcgcggacgg
     4681 tcgcgatggc gcccggttcg ttcgcggcga ggattatctc ggcaccgaat ccggcggcgt
     4741 cctcgtgctg gacgacgtcg agcgcctggg cgaggcaagg caggtggcgc tgttcaatgc
     4801 cttcaacgag cgtgctttcg cgctcatcgt cgtctccgcc cacgccgcgc ccagggacgt
     4861 ggcgctgcga cgcgacctcg ccacgcgcct cgcgaccggc ttcacctatc gcctgcttcc
     4921 cctcacggac gaagagaaga gccaggcgct ggcggcccac gcacgggcgc gcggcttcgc
     4981 gctctccgac gacgtggcgg cctacctcct cacgcacgcg cggcgcgaca tgccctcgct
     5041 catccgggcg ctggacgcgc tcgaccggta ttcgctcgaa accggccggc ccatcaccgt
     5101 gccgctcctg aaggcggcgc tgcagcccga gctcacgtga aactcgcgct attcgatctc
     5161 gacaacacgc tgctcgacgg ggacagcgac tattgctggg cccagtacct catcgagcgc
     5221 ggcatcctcg atcgcgaggg ttacgagaag aagaacgact ggttctacca ccactacaag
     5281 cagggcacgc tcgacatcca cgattacctc gatttccagc tcgcgcccat cgccgggcgg
     5341 ccgcgcgagg tgatcgacga gtggcaccgc gaattcatgg agcgcaagat ccggccgatc
     5401 atccacgcgg gaaccccggc gctcatcgcc tcccacggcg acgcgctgaa ggccgtcgtg
     5461 acggccacca accgcttcat caccgagccc atcgcccgcg agctcggcgt ggagcacctc
     5521 atcgcgtgcg acgtggagtt gatgcccgac ggccggctta ccggaaggcc gacgggaacg
     5581 ccgtccttcc gcgaaggcaa ggtcgcgcgg ttgcacgagt ggctggccac gctgggccgg
     5641 cgcttcgagg atttcgacga gacgtggttc tacagcgatt cgcacaacga cctgccgttg
     5701 ctcgagatgg tatcgaagcc ggtggcggtc gatcccgatc cgacgctcga aagccacgcc
     5761 cgcgaacgcg gctggcccat cctgaggttg cgcgcatgat caagtcattc atctcccggg
     5821 tcttcggccg cggcgcgaag caggccgcgc acccgtcgaa gccggtcatc tacggcccgg
     5881 acaagcacca catccgcaag gaccacatca gccgcggcgc gcgcaagacg tgcgaggaac
     5941 tgcagcgcgc gggacacacg gcgttcgtgg tgggcggcgc ggtgcgcgac ctgatcctcg
     6001 gcatgcagcc caaggatttc gacgtggcca ccagcgccct gccggacgag gtgcgctcga
     6061 tcttccggcg ctcgcgcatc atcggccggc gcttccagat cgtgcacgtg atgtggggcg
     6121 ccgagaccgt ggaggtttcc acctaccgcg cgcacttcac gacgaaggac cccgaggcgg
     6181 aagtcgagat cgacaagcag gacgagcacg gccgcgtgct ctccgacaac gtcttcggca
     6241 cgcaggccga ggacgcggtg cgccgcgatt tcaccgtgaa cgcgctcttc tacgacccgg
     6301 tgaaggagga ggtgtgggac taccagcacg gcgtgcggga cctggtcgcg aggaagctcg
     6361 tgacgatcgg cgaccccacc acgcgctttc gcgaggatcc ggtgcggatg ctgcgtgccg
     6421 cgcggttctc cgccaagctc ggcctggcca tcgacccgtc gacggccgcg cccatcgcat
     6481 cgctcaagca cctcctggaa aacgtcccgc aggcgcggct cttcgaggaa atcctcaaga
     6541 tgctgctttc cggcaacgcg gtggcctgcg tgcagcggct gcgcgacctg gacctgcacc
     6601 acgggctgct tccgttgctg gacgatgccc tcgacgatcc ggccacggga cccttcgccc
     6661 tggccgcgct caaggccacc gacgaccggc tgcgcggcga caagccggtt tcgcccgcgt
     6721 tcctgctcgc ggccctcctg tgggggcgcg tcgagcggcg ctggcacgaa ctcgaggaaa
     6781 agggccagcc gtcaacgcct tccctgcatt cggccatgca cgatgcgctg gacgcgcagc
     6841 gcgaatcgct tgccattccg cgccgcttcg atgcaacgat gaaggaactc tggctgctgc
     6901 agccgcgctt catgcagcgt ggcggcacgc ggcccttccg cctggtggag cacccgcggt
     6961 tccgcgccgc ctacgatttc ttcgagttgc gtgcgcagtc cggcaatgcg ccgctggagg
     7021 ccgccgaatg gtgggcgcga ttccagaaag cctccggcga cgagcgcagc cgcatgctgc
     7081 acgccgacga ggggccgaag aagaagaagc gccgccggcg cggcggcaag cgccaccccg
     7141 aggacggcgg ctcccccgag gaagtggcgc cgggcagcga cgaatgaacg ccgtccgtgc
     7201 ggtggttgcg ctcgggtcca acctcgacgg ccccgaggca cgcgtgcgcc tggcgttcga
     7261 cgaggtgggc gtgctgccct cgacgcggct gctggcacgt tcggccctct accggaccgc
     7321 gcccgtgggc tacctggacc agccggcgtt cgtgaatgcc tgcgcgctgg tcgaaacctc
     7381 gctcgggccg cgcgaactcc tcgagggcct gctcgccatc gagcgccgcc acggacgcgt
     7441 gcgcgacata cccgacgggc cgcgcacgct ggatctcgac attgccctct acgacgacct
     7501 cgtcatgcac gagcacggcc tcacgctgcc gcatccgcgg gcgcacgagc gcgccttcgt
     7561 gctggtgccg ttgctcgacg tgtggcccga tgccgtcatt cccgggcgtg gcgcggccgc
     7621 gtccctgcgc gatcgcgttg gcgaccaggc catcgagagg ttgccggaca acccatgaga
     7681 caccacttca tcgtggtcga aggtcccatc ggctgcggca agacgagcct ggccaagctg
     7741 ctcgccaggc gcctcaatgc aggcctgatc ctggaggagc cggaagccaa cccgttcctt
     7801 ccgctcttct accgcgacat gcgccgccac gccctttcca cgcagctctt cttcctgttc
     7861 cagcgattgc accagctcga aggtctgcgc cagcccgacc tgttcggcaa gcccacggtc
     7921 gccgacttcg cgctgcagaa ggacccgctc ttcgcccgcc tgacgctcga cgacaacgag
     7981 ttcgggctgt acacgcgcat cttcgaccac gtgcggccgc aggcccccac ccccgatctc
     8041 gtggtctacc tccaggccaa ggtggagacg ctcgtggagc gggtgcgccg ccgcggcaat
     8101 ccgatggagg cgggaatctc cgacgagtac ctgcgcgcga tctccgacac ctacacccgc
     8161 tacttctaca actacggcga gagcgcgctc ctcatcgtga acagcgagcg gctcaatttc
     8221 gtcgacgtgc cggagcactt cgacatgctc gtcgagcgcg tgaagtcgat ccgcggcggg
     8281 cgcgagttct tcaaccgcgc gtagcggaga acgcgacggc ggcaaagtcg agggcaatcg
     8341 tgggcatgtg aggtgaggcg gtggtggaag tcgatcgcga tcctgggcgc gccagttgag
     8401 gaggcggcgg c
//
LOCUS       DASUEV010000263         8525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1658723, whole genome shotgun sequence.
ACCESSION   DASUEV010000263 DASUEV010000000
VERSION     DASUEV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658723"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..2907)
                     /gene="mgtA"
                     /locus_tag="VFV55_08525"
     CDS             complement(361..2907)
                     /gene="mgtA"
                     /locus_tag="VFV55_08525"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEX4944385.1"
                     /translation="MGDLHNEGGTAWWSVAVADLLASLAAGAQGLSPAEARTRLRTAG
                     PNLVTEERRFGWARLLLSQFTSPLVLILVFAGLVSLVLRDWVEAAIILAIVAGSALLG
                     FVQEFRASKAVAELQGRIALGVRVRRGGEILAVPARDLVPGDMVLLAAGNLVPADGRV
                     LEARDFLVSEAALTGESFPVEKRPADLPPGTPLAARTNSAFLGTSVRSGTATMLVVRT
                     GRFTALGEIAARLGSRVPETEFARGIRQFGYLLVRVMVVMVVFVMAVNQWLGRPVLES
                     LLFAVALAVGISPELLPAIMSVTLSRGARHMARSGVIVRRLEAIENLGSMDILCTDKT
                     GTLTEGLVTLDAALDAQGQDSPEVRRLAHLNAAFETGIENPLDTAIVEAGKRDGLTTA
                     GWRKVDEIPYDFIRKRLTIVAEPEGNASAHLMITKGAFDTVLAASSAVDDAGTVRPLD
                     ATLRSTLTALHRDMGEAGFRALALARREVDARDRYEREDERDLVFMGFLRFADPPKPD
                     AQDTIRDLAAMGVSVKIITGDNRWVAGHIASVIGLDHATMLTGEQVAAMPDESLWHLA
                     GNTALFVEVDPQQKERIVRALQHGGHAVGYLGDGINDAPALHAADVGISVDGAVDVAR
                     ESADVILLRRDLNVLRRGIEDGRHTFANTLKYIKITTSANFGNMVSMALATPLLPFLP
                     LAAKQILLNNFLSDLPSVAISTDHVDRQELAGSQRWDVTEVRRFMVVFGLLSTAFDLL
                     TFYLLLRVFDTGEAEFQTVWFIVSLLTELAVVFVLRTRGPAFASRPSALLAGSSLLVA
                     AAALAVPWLGPVSTAFGFVPVSAGLFGASILVVVAYIVATEAAKRHFYRH"
     gene            complement(2988..3335)
                     /locus_tag="VFV55_08530"
     CDS             complement(2988..3335)
                     /locus_tag="VFV55_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944386.1"
                     /translation="MKRTAVVLAFVLGTTAAFAQAPAAPAAPAAPVVEPARCEPKPVY
                     PGIKAIQDDKKREAFQQVLKGYQDCIKAYVAERKAFVEASNAAIRAAVEEHNAVMNKF
                     REDQEAAKKELGQ"
     gene            complement(3424..3924)
                     /locus_tag="VFV55_08535"
     CDS             complement(3424..3924)
                     /locus_tag="VFV55_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944387.1"
                     /translation="MHPRDRAWRPSAAAFLLAAAILPATAADKPCSSADKAIDGVTTW
                     AALQKAVQDFGPCDKGPTGDLFTEAILRVIISGWPQVGDAEPILEKDKAFSDWLNRRL
                     SSPSLSTQDTAEIRDLAKASCPKGREKVCAQLLSAVEMGRALSAPELLQLTPMPATPA
                     PAKGKP"
     gene            complement(3893..5791)
                     /gene="dxs"
                     /locus_tag="VFV55_08540"
     CDS             complement(3893..5791)
                     /gene="dxs"
                     /locus_tag="VFV55_08540"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX4944388.1"
                     /translation="MSAYPLLEAINDPADLRRLDRKQLPALAVELRRFIVESVAATGG
                     HLSSNLGTVELTIALHYVFATPEDRIIWDVGHQTYGHKILTGRRAGMARLRQKGGIAG
                     FPRREESPYDAFGTAHSSTSISAALGMAVASRHLGRDNRAIAVIGDGAMTGGMAFEAL
                     NNVKQSGANVIVVLNDNDMSISENVGALNNYLARLMSGRFYAATKKTADRILSVSPPM
                     KELAKRFEGHAKGMLTPSTLFEEFGLNYTGPVDGHNIDALVDTLTNVKRLDGPQFLHV
                     VTKKGQGYAFAEDDPIAYHGPGKFDPGVGLVKPVTASGARPGPTYTQVFGDWLCDMAK
                     LDRRLVGITPAMREGSGMGRFSREYADRYFDVGIAEQHAVTFAAGLACDGMKPVVAIY
                     STFLQRAYDQLIHDVALQNLPVVFALDRGGIVGADGPTHHGAFDLSYLRCIPNMTVMA
                     PADENECRQMLYTGFMLDGPAAVRYPRGTGPGTEVLAQMKALPVGQGEIRREGRKVAI
                     LAFGSMLKPALEAGAELGATVANMRFVKPLDDALVLRLATTHDLVVTVEENVLEGGAG
                     SAVLESLAAQGVEVPVLQLGLPDHFVEHGDPAQLLADCVLDAPGIVRSIRERLLQGNA
                     SQRPGMAA"
     gene            complement(5992..6840)
                     /locus_tag="VFV55_08545"
     CDS             complement(5992..6840)
                     /locus_tag="VFV55_08545"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX4944389.1"
                     /translation="MATVLAGVLPSPRIAPARLHDAMRYATLGDGKRVRPMLVFAAGL
                     VSGADPARLEIASAAVELIHAYSLVHDDLPCMDDDVLRRGKPTCHVEFDEATALLAGD
                     AMQSLAFQLLSENRLADDPAVQLQMIQLFAVACGSRGMAGGQAIDLDAVGKSLTLPEL
                     ENMHIHKTGALIRAAVLLGAHCGQSVDTASLGSLDRYAKCVGLAFQVVDDVLDEEGDS
                     ATLGKTAGKDRDAGKPTYTSLLGVPEARKFADELLAEAHEALSAFDDRAGRLRELADF
                     IVKRDR"
     gene            complement(6885..7121)
                     /locus_tag="VFV55_08550"
     CDS             complement(6885..7121)
                     /locus_tag="VFV55_08550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX4944390.1"
                     /translation="MPTTPPPANDLPFEKALEELESLVARMEDGKLPLEESLAAYQRG
                     AELLRHCEGKLSAAQARISVLEGEVLKDLPAADS"
     gene            7377..8492
                     /locus_tag="VFV55_08555"
     CDS             7377..8492
                     /locus_tag="VFV55_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX4944391.1"
                     /translation="MSDLASQLALTRTPIHLPLSWYFDPKVAEIEQKLLFDHGPGYVG
                     HAMMAPRPGDYRVLDWRENGPGWVLVNNDGRHDMISNICRHRQAVMLDGAGQLPGGTI
                     TCPVHRWAYDSAGKHIGAPHFPEHPCLDLPKRDLQQWHGMLFNGPRDIARDLASMSVA
                     GDLDFDNYVLDKVEVVNYDFNWKTFIEVYLEDYHVAPFHPGLGHFVTCDDLKWEYGER
                     FSVQTVGVNKALSEPGSAVYARWHEQVLKYRRGEPPPHGAIWLTYYPNIMVEWYPHVL
                     VISTLWPRGPEKCVNVVEFYYPEEIALFEREFVEAEQKAYDETAVEDKEICDRMTQGR
                     RALWLEGREEHGPYQSPMEDGMVHFHEFIRREIAQNL"
BASE COUNT         1385 a         2790 c         2967 g         1383 t
ORIGIN      
        1 ctcgaatggt ctaccgtccc catcgaacac gtccgtgaat gcctaagtaa cgccatcggc
       61 agcatcggcg accactcgaa tcggcacggc gtgtccattg gggccgggga tggggcaagt
      121 cttccggcat ccttggcacg cgaccgccgg gatgggaaag cgcagcggcc ccatctcttc
      181 atgggtaaaa agtcggggcc tggcttgttc tgcctggccc cgactttttg ggacccatgc
      241 gacggtcacg attgcgtgcc caggatgccg gaagacttgc cccatccccg gcccatttcc
      301 gcccccatcc ccggccaacc cacttcccca tccccctcgc aaaccctatt ggcccgcaca
      361 tcaatggcgg tagaagtgcc gcttggccgc ctcggtggcg acgatgtaag cgacgacgac
      421 caggatcgac gcgccgaata gccccgccga aactggcacg aacccgaacg cggtcgagac
      481 cggtccgagc cacggaacgg cgagcgcagc ggcggcgaca aggagcgagg agcccgcaag
      541 cagtgcgctc gggcggctgg cgaatgccgg gccccgcgtg cgcagcacga acaccaccgc
      601 cagttccgtc aacagggaaa cgatgaacca caccgtctgg aattcggcct cgccggtgtc
      661 gaagacgcgc agcagcaggt agaaggtgag caggtcgaac gcggtgctga gcagcccgaa
      721 taccaccatg aaccggcgca cctcggtcac gtcccagcgc tgcgagccgg cgagctcctg
      781 gcggtcgacg tggtcggtgg agatcgcgac cgagggcagg tcggagagga agttgttgag
      841 caggatctgc ttggcagcca gcggcaggaa gggcaggagc ggcgtggcca gggccatgct
      901 caccatgttg ccgaagttgg cgctggtcgt gatcttgatg tacttgagcg tgttggcgaa
      961 ggtgtgccgg ccatcctcga tcccgcggcg cagcacgttg aggtcccggc gcaggaggat
     1021 gacgtccgcg ctctcgcgcg ccacgtccac tgcgccatcc acggagatgc ccacgtcggc
     1081 cgcgtgcagc gccggggcgt cgttgatgcc gtcgccgagg tagcccaccg catggccgcc
     1141 gtgctgcagc gcgcggacga tgcgttcctt ctgctgcgga tcgacttcga cgaagagggc
     1201 ggtgttcccg gcgaggtgcc acagcgactc gtcgggcatc gcggcgacct gttccccggt
     1261 gagcatcgtc gcgtgatcca ggccgatgac gctcgcgatg tgaccggcca cccagcggtt
     1321 gtcgccggtg atgatcttca cgctgacgcc catcgccgca aggtcgcgga tcgtgtcctg
     1381 tgcatcgggc ttcggcgggt ccgcgaagcg caggaacccc atgaagacga ggtcgcgctc
     1441 gtcctcgcgt tcgtaccggt cgcgggcatc gacttcccgg cgggcgagcg cgagcgcgcg
     1501 gaaccccgct tcccccatgt cgcgatgcag tgcggtgagg gtggatcgca gggtggcgtc
     1561 cagcggccgc acggttcccg cgtcgtccac ggcgctgctc gcggcgagaa ccgtatcgaa
     1621 tgcgcccttg gtgatcatga ggtgtgcgct ggcgttgcct tccggctcgg cgacgatggt
     1681 caggcgcttg cggatgaagt cgtaggggat ctcgtcgacc ttcctccatc ccgcggtggt
     1741 aagaccgtcg cgcttgccgg cctcgacgat ggccgtatcg agcgggttct cgatacccgt
     1801 ttcgaaagcg gcgttcaggt gggccagccg ccggacctcg ggcgagtcct gcccctgcgc
     1861 gtcgagcgcg gcgtccagcg tgaccaggcc ctcggtgagc gtgcccgtct tgtcggtgca
     1921 caggatgtcc atggacccga ggttctcgat cgcctcgagc cggcgcacga tcacgccgct
     1981 gcgcgccatg tgccgtgcgc cgcgcgacaa cgtgacgctc atgatcgccg gcagcagttc
     2041 cggcgagatg cccacggcga gcgcgacggc gaagaggagg gactcgagca ccggtcgccc
     2101 gagccactgg ttcaccgcca tcacgaagac caccatcacg accatcaccc gcacgagcag
     2161 gtagccgaac tggcggatgc cgcgcgcgaa ttccgtctcc ggaacgcgcg agcccagtct
     2221 tgccgcgatt tcccccagcg cggtgaatcg cccggtgcgc acgacgagca tcgtggccgt
     2281 gccgctgcgc accgaggtgc cgagaaacgc gctgttggtc cgcgcggcca gcggcgtgcc
     2341 ggggggcagg tcggcgggcc gcttctcgac ggggaaggat tcccccgtga gcgcggcctc
     2401 gctcaccagg aaatcgcgcg cctcgaggac gcggccatcc gcggggacga gattgccggc
     2461 ggcgagcagg accatgtcgc ccggaaccag gtcgcgcgcg ggcaccgcga ggatctcgcc
     2521 gccgcggcgc acgcggacgc cgagcgcgat ccggccctgc agctcggcga ccgccttcga
     2581 ggcgcggaac tcctgcacga acccgagcag ggcactgccc gccacgatcg cgagaatgat
     2641 cgcggcctcg acccagtcgc gaaggacgag ggagacgagc ccggcgaaaa cgaggatgag
     2701 aacgagcggg ctggtgaact gggagaggag cagccgcgcc catccgaaac ggcgctcctc
     2761 ggtgaccagg ttcggcccgg ccgtgcgaag gcgcgtgcgc gcctcggcag gcgaaagccc
     2821 ctgcgcgccg gcagcgaggc tggccaggag atccgcgacg gccacgctcc accaggccgt
     2881 gccgccttcg ttgtgcaggt cgcccacgga atcgctcggt gcaatccggg aaattcaggc
     2941 tatcccgcgg cctccccggc tccgtttacc ggtgcagcgc gttcggacta ctgccccagc
     3001 tccttcttcg ccgcctcctg gtcctcgcgg aacttgttca tgacggcgtt gtgctcttcc
     3061 accgcggcgc ggatcgccgc gttgctcgcc tcgacgaatg ccttgcgctc cgccacgtag
     3121 gccttgatgc agtcctggta gcccttgagt acctgctgga atgcctcgcg cttcttgtcg
     3181 tcctggatgg ccttgatgcc ggggtagacg ggcttcggtt cgcatctggc cggctcgacc
     3241 acgggggccg cgggggcggc cggagcggcg ggtgcctgcg cgaaggcggc ggttgtgccc
     3301 aggacgaagg caagcacgac ggcggtgcgt ttcatcatcg aaatcctttc ggtcgcgagg
     3361 gtgcggattc tagcttggat cgtgtgtgca gggagccggg gaaggtcctg cgccgggggc
     3421 agcctacggc ttgccctttg cgggcgcggg ggtcgcgggc atgggggtca gttgcaggag
     3481 ctcgggcgcg gaaagagctc gccccatctc caccgcggag agcagctgcg cgcagacctt
     3541 ctcgcggccc ttggggcagc ttgccttggc aaggtcgcgg atctcggcgg tgtcctgcgt
     3601 cgagagcgaa gggctgctca gccgccggtt gagccagtcg ctgaacgcct tgtccttctc
     3661 caggatgggc tcggcatcgc ctacctgcgg ccacccgctg atgatcacgc gcaggatggc
     3721 ctcggtgaac aggtccccgg tcggaccctt gtcgcacggg ccgaaatcct ggaccgcctt
     3781 ctgcagtgcc gcccaggtgg ttacgccgtc gatcgccttg tcggcactgc tgcagggttt
     3841 gtcggcagcg gtcgcgggaa ggatcgcggc cgccaggaga aaagccgcgg cgctaggccg
     3901 ccatgcccgg tctctgggat gcattgcctt gcaatagcct ttcgcggatg gaacgaacga
     3961 tgccgggggc atcgaggacg caatcggcga gcaattgcgc cggatcgccg tgttcgacga
     4021 agtgatcggg aaggcccagc tgcaggaccg gaacctcgac gccctgggcc gcgagggatt
     4081 cgaggaccgc ggatcccgcg ccgccctcga gaacgttctc ctcgaccgtg acgacgagat
     4141 cgtgggtggt ggcgagccgc agcacgaggg catcgtccag cggcttcacg aagcgcatgt
     4201 tggccacggt ggcgcccagt tccgcacccg cttccagggc cggcttgagc atcgacccga
     4261 aggcaaggat cgcaaccttg cgcccttcgc ggcggatttc gccctggccc accggaagcg
     4321 ccttcatctg cgcgagcacc tcggtgccgg ggccggtgcc acgggggtag cgcaccgcgg
     4381 cgggaccgtc gagcatgaaa ccggtataga gcatctggcg gcactcgttc tcgtcggcgg
     4441 gcgccatcac cgtcatgttg ggaatgcagc gcaggtacga gaggtcgaag gcgccgtggt
     4501 gtgtcggccc gtcggcaccc acgatgccgc cgcgatcgag agcgaagacc accggcaggt
     4561 tctgcagcgc aacgtcgtgg atgagctggt cataggcgcg ctgcaggaac gtggagtaga
     4621 tggcgaccac gggcttcatg ccgtcgcacg cgaggcccgc ggcgaacgtg accgcgtgct
     4681 gctccgcgat gcccacgtcg aaatagcgat ccgcgtactc gcgggaaaag cgccccatgc
     4741 ccgagccctc gcgcatcgcc ggcgtgatgc ccaccaggcg gcggtcgagc ttcgccatgt
     4801 cgcaaagcca gtccccgaag acctgcgtgt acgtgggccc gggcctggcg ccggatgcgg
     4861 tgacgggctt cacgaggccc acgcccgggt cgaacttccc ggggccgtgg taggcgatcg
     4921 ggtcgtcctc ggcgaaggcg tagccctggc ccttcttggt caccacgtgc aggaactgcg
     4981 ggccgtcgag gcgcttcacg ttggtgagcg tgtcgacgag ggcgtcgatg ttgtggccat
     5041 cgaccggccc ggtgtagttg aggccgaact cctcgaagag cgtcgagggc gtgagcatgc
     5101 ccttggcgtg cccctcgaag cgcttggcga gctccttcat gggcggcgac acggacagga
     5161 tccggtcggc cgtcttcttc gtggccgcgt agaagcgccc cgacatcagg cgggcgaggt
     5221 agttgttgag cgcgcccacg ttctccgaga tcgacatgtc gttgtcgttc aggaccacga
     5281 tcacgttggc gccggactgc ttcacgttgt tgagcgcctc gaaggccatg cccccggtca
     5341 tggcgccgtc gccgatcacg gcgatcgcgc ggttgtccct gccgagatgc cgggacgcca
     5401 ccgccatgcc cagcgcagcg gaaatggaag tcgacgagtg cgcggtgccg aaggcgtcgt
     5461 agggcgattc ctcgcgccgc gggaagccgg cgatgccgcc cttctgccgc agcctcgcca
     5521 tgccggcgcg ccgcccggtg aggatcttgt ggccataggt ctggtgaccc acgtcccaga
     5581 tgatgcggtc ctcgggggtc gcgaacacgt aatgcagcgc gatggtgagc tccaccgtgc
     5641 ccaggttcga agacaggtgc ccgcccgttg ccgccacgga ctcgacgatg aaccggcgca
     5701 gttccacggc gagcgcggga agctgcttgc ggtcgagccg ccggagatcc gcggggtcgt
     5761 tgatcgcttc gagtaagggg taggcgctca ttgtcgaggt tcggccgccc gggccgggag
     5821 agggacattt tgccgcatct ggcgcccgcc cgcctgcgcc gcctcaaatg gtcgaaagag
     5881 gggtcaggtt actttgcaca agaaataggc aaacgggaaa ggggtcaggt tacttttcaa
     5941 ctcgccgtct cgcggcggca atgcgacaga aaagtaacct gacccctttt cctaccggtc
     6001 gcgcttgacg atgaagtccg ccagctcgcg caggcgcccg gcgcggtcgt cgaacgccga
     6061 gagcgcctcg tgggcctcgg cgaggagttc gtccgcgaat ttcctggcct cgggcacgcc
     6121 gagcaggctg gtgtaggtgg gtttcccggc atccctgtcc ttgccggccg tctttccgag
     6181 ggtggcggag tcgccttcct cgtccagcac gtcgtccacg acctggaagg cgaggcccac
     6241 gcatttcgcg taccggtcga ggctccccag ggacgcggtg tcgacggact ggccgcaatg
     6301 ggcacccagc aggacggcgg cccggatcag ggcgcccgtc ttgtggatgt gcatgttctc
     6361 gagctccggc agggtgagcg acttgcccac cgcatcgagg tcgatcgcct ggccgccggc
     6421 cattccgcgc gacccgcacg ccacggcgaa gagctggatc atctgcagct gcaccgcggg
     6481 atcgtcggca aggcggttct cggacaggag ctggaatgcg aggctctgca tggcgtcgcc
     6541 ggcgaggagc gccgtggcct cgtcgaattc cacgtggcag gtgggcttgc cgcgcctgag
     6601 cacgtcgtcg tccatgcagg gcaggtcgtc gtgcaccagc gaatacgcgt ggatcagctc
     6661 gacggcggcc gaggcgatct cgagccgggc cggatcggcc ccggaaacga gtccggcggc
     6721 gaacacgagc atgggccgca cgcgcttgcc gtcgcccagc gtcgcgtaac gcatcgcgtc
     6781 gtgcagtcgc gcgggcgcga tgcgggggga gggcaggacg ccggccagca cggtcgccat
     6841 gcggccagcc acctcgcgcg accaggtcgg gaagtcggtg gcgctcacga gtcggccgcg
     6901 ggaaggtcct tcagcacctc tccctcgagc acgctgatgc gcgcctgtgc ggcggaaagc
     6961 ttgccctcgc aatggcgcag caattccgcc ccgcgctggt aggcggcgag ggattcctcg
     7021 agcggaagct tgccgtcctc catccgggcc acgagggact cgagttcctc gagggccttc
     7081 tcgaagggga ggtcgttggc cgggggcggg gtcgtgggca tgggtcggca ccttagcgca
     7141 aatggtccgc ggcgtcattt tcgtgccacg cgcaggtatt cccgcaagat tatgccgccc
     7201 gttgacttca ctcaaggcag cgcatggaaa tccttgaaaa atgagggaac cttgggcaga
     7261 ctggcctgct caaattcatt tcgtgctggt tcgggacgga gctgcagagt taaaagctgg
     7321 ctgactcgat tagcccggtg tggttttcat ctttggtgtc atcggaggtg tggaacatgt
     7381 cggacttggc gagccaactg gcgctcaccc ggacgcctat tcatctgccg ctttcgtggt
     7441 acttcgaccc gaaagtggcg gaaatcgagc aaaagctcct cttcgaccac ggtccgggct
     7501 atgtcggcca cgccatgatg gcgccccggc ccggggatta tcgcgtcctc gactggcgcg
     7561 agaacggccc cggctgggtg ctcgtgaaca acgacgggcg ccacgacatg atctcgaaca
     7621 tttgccggca ccggcaggcg gtgatgctcg acggcgcggg ccagcttccc ggcggcacca
     7681 tcacctgccc cgtgcatcgc tgggcctatg acagcgcggg caagcacatc ggggcgcctc
     7741 actttcccga acacccgtgc ctcgacctgc cgaagcgcga tctccagcag tggcacggca
     7801 tgctcttcaa cgggccgcgc gacatcgccc gggacctggc gtcgatgagc gtggccggtg
     7861 acctcgattt cgacaactac gtcctcgaca aggtcgaggt cgtgaactac gacttcaact
     7921 ggaagacctt catcgaggtc tacctcgagg actaccacgt cgccccgttc cacccgggcc
     7981 tgggccactt cgtgacctgc gacgacctca agtgggaata cggggagcgc ttcagcgtgc
     8041 agaccgtggg cgtgaacaag gcgctatcgg aacccggcag cgccgtgtat gcgaggtggc
     8101 acgagcaggt gctgaagtac cggcgcggcg agccgccgcc ccacggcgcc atctggctca
     8161 cgtactaccc gaacatcatg gtcgagtggt acccgcacgt gctggtgatc tcgaccctct
     8221 ggccgcgcgg gccggaaaag tgcgtgaacg tggtcgagtt ctactacccc gaggagatcg
     8281 cgctcttcga gcgcgagttc gtggaggccg agcagaaggc ctacgacgag acggcggtgg
     8341 aggacaagga aatctgcgac cgcatgacgc agggccgccg cgcgctgtgg ctcgaggggc
     8401 gcgaggagca cggaccgtac cagtccccga tggaggacgg catggtccac ttccacgagt
     8461 tcatccggcg cgaaatcgcg cagaacctct aggcaggaaa aggggtcagg ttactttgag
     8521 gtaaa
//
LOCUS       DASUEV010000264         5026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1572241, whole genome shotgun sequence.
ACCESSION   DASUEV010000264 DASUEV010000000
VERSION     DASUEV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572241"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="hemE"
                     /locus_tag="VFV55_08560"
     CDS             complement(<1..1035)
                     /gene="hemE"
                     /locus_tag="VFV55_08560"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX4944392.1"
                     /translation="MQNDNFLRALLRQPTDYTPVWIMRQAGRYLPEYNATRRKAGSFL
                     ALAKTPELACEVTLQPLERFALDAAILFSDILTIPDAMGLGLYFADGEGPKFERPVRT
                     EEDVKRLGTADLDSLRYVFDAVSLIRKSLAGRVPLIGFSGSPFTLACYMVEGGGSDDF
                     RLVKSMLYARPDLLHRILELNAVSVTAYLNAQVAAGAQAVMVFDTWGGALSDAAYREF
                     SLAYAKKVFAGVTRAAEGRTVPRVLFTKGGGLWLEPMTEAGVDALGLDWTIDIGAARR
                     RVGDKVALQGNMDPAVLFAAPDAIEAEAGRILGSFGKGPGHVFNLGHGVSQFTPPEHV
                     QALVEAVHRLS"
     gene            complement(1081..1866)
                     /locus_tag="VFV55_08565"
     CDS             complement(1081..1866)
                     /locus_tag="VFV55_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4944393.1"
                     /translation="MLAYAGMAAMLWLAQESILFYPQPVRGSPAAPPGWSLEEVALTA
                     ADATPLRGVLLLPPGEPASRRAVVIYFGGNAEEVTAYAPEAERHYGRRAVLLVNYRGY
                     GKSGGRPGEKALVADALAIHDWAVKRADLDPGRICVHGRSLGSGVAVQLAAARPLRCI
                     VLTSPFGNLAQVGKSHYPWLPVGWLLRHRFESAQHARHLKIPALVIYGGADTIIGPEH
                     SERLADAWGVPAERLRIEGLGHNDLDLDPRYARTIAAFLDRTL"
     gene            complement(1914..3359)
                     /locus_tag="VFV55_08570"
     CDS             complement(1914..3359)
                     /locus_tag="VFV55_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX4944394.1"
                     /translation="MGKITGFMEIARKDRGYAPVAERIKVYKEFIRPLTDADMSLQGA
                     RCMDCGIPFCQTGCPVNNVIPDWNDLVYRNNWKSALEVLHSTNNFPEFTGRVCPAPCE
                     AACTLNINDDPVAIKSIEQAIVDKGWAEGWIVPEVPANKTGRTVAVVGSGPAGLACAQ
                     QLARAGHDVTLFEKSDRIGGLLRYGIPDFKMEKLLIDRRIEQMGAEGVTFRTGVNVGV
                     DLKADDLVAGFDAVVLSGGSEKPRDLPVPGRELAGIHFAMDFLPLQNKVVAGDKVPGQ
                     ILATGKHVVVIGGGDTGSDCIGTSIRQGAKSVVNFELLPRPPEQEDKPLTWPYWPLKL
                     RTSSSHEEGAERDFAVATKRFEGKGGKVASLTACHVEWVKDEKGGMSMKEVPGTEFTI
                     PAELVLLAMGYVHPVHEGLVAGLGVEKDARGNVKAGTEGVGCYATSVAKVFAAGDMRR
                     GQSLVVWAIREGRQCAREVDAFLMGSSDLPR"
     gene            complement(3373..>5026)
                     /locus_tag="VFV55_08575"
     CDS             complement(3373..>5026)
                     /locus_tag="VFV55_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX4944395.1"
                     /translation="PPPHHDIYSIEDLAQLIHDLKNVNPKARVSVKLVSEIGVGTVAA
                     GVAKAKADHVTISGFDGGTGASPLSSIKHAGSPWEIGLAETQQTLVLNKLRSRICVQT
                     DGQMKTGRDVVVGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPELIKK
                     FTGKPEHVINYFFFVAEEVREWMAKMGFRRFEDMIGRSDLLDMRRGIEHWKAKGLDFS
                     RLFHRPTVDASVPIHCTQAQDHALEKALDHQLIEKVRPALESKRPVQLESAIFNRNRT
                     VGAMLSGEVARRHGHAGLPEDTIHVKFTGTAGQTFGGWLARGITFELEGDANDYVGKG
                     LCGGRIVVYPPKDCPIVPEENIIVGNTVMYGAISGEAYFRGVAGERFAVRNSGATAVV
                     EGVGDHGCEYMTGGTVVVLGTAGRNFAAGMSGGIAYVLDEEGDFTQRCNLAMVELEPI
                     PEEDRMAADAGANVELETHGKVQVDHLAGNDEATIKSLIQCHLLYTGSERARRILENW
                     AAYLPRFVKVMPIEYKRALGEMALEQARRGEAAGVVEVRAND"
BASE COUNT          744 a         1701 c         1765 g          816 t
ORIGIN      
        1 ggagaggcga tggacggcct cgacgagggc ctggacgtgc tcgggcgggg tgaactgcga
       61 aaccccgtgg ccgaggttga aaacgtgccc cgggcccttc ccgaagctgc ccaggatgcg
      121 cccggcttcg gcctcgatgg catccggggc ggcgaaaagc accgccggat ccatgttgcc
      181 ctgcagcgcc accttgtcgc ccacgcgccg gcgcgcggcg ccgatgtcga tggtccagtc
      241 gagtcccagc gcgtccaccc ccgcttcggt catgggctcg agccacagcc cgccgccctt
      301 ggtgaagagg acgcgcggca ccgtgcggcc ctcggcggcg cgcgtgacgc ccgcgaacac
      361 cttcttcgcg taggcgagcg agaactcgcg gtaggccgca tccgaaagcg ccccgcccca
      421 ggtgtcgaag accatcaccg cctgcgcgcc cgcggcgacc tgcgcattga gatacgcggt
      481 gacggaaacg gcgttgagct ccaggatgcg gtgcaggagg tcgggccgcg cgtagagcat
      541 cgatttcacg aggcggaaat cgtcgctgcc gccgccttcg accatgtaac aggcgagcgt
      601 gaaggggctt ccggaaaaac cgatcagcgg cacgcggccg gcgagcgact tgcggatgag
      661 ggacaccgcg tcgaagacgt agcgaagcga gtcgaggtcg gcggtaccga ggcgcttcac
      721 gtcctcctcg gttcgcaccg gacgctcgaa tttcgggccc tcgccgtcgg cgaagtagag
      781 gcccagtccc atcgcgtccg ggatggtgag gatgtccgag aagaggattg ccgcgtcgag
      841 cgcgaagcgc tccagcggct gcagcgtcac ctcgcaggcg agctcgggcg tcttcgcaag
      901 ggcgaggaag ctgccggcct tcctgcgcgt ggcgttgtac tcgggcaggt agcgcccggc
      961 ctggcgcatg atccagacgg gcgtgtagtc ggtgggctgg cgcagcaggg cgcgcaggaa
     1021 gttgtcgttt tgcatggggt gcgattctct cacggcgggc ggggcggccc gcgacaacgg
     1081 tcaaagggtc cggtcgagga aagccgcgat cgtccgggcg tagcgcgggt cgaggtccag
     1141 gtcgttgtgg ccgagcccct cgatgcgcag gcgctccgcc ggaacccccc aggcatccgc
     1201 gaggcgctcc gaatgctccg ggccgatgat cgtgtcggcc ccgccataga tcaccagcgc
     1261 ggggatcttc aggtggcgcg cgtgctgcgc ggattcgaat cgatgccgca ggagccaccc
     1321 tacgggcagc cagggatagt gggacttccc cacctgcgca agattcccga atggggaggt
     1381 gagtacgatg cagcgcagcg gccgcgcggc cgcgagttgc accgccacgc cgctgccgag
     1441 gctgcgcccg tgcacgcaga tccggccggg atccaggtcg gcacgcttca cggcccagtc
     1501 gtggatggcg agcgcgtcgg ccacgagagc cttctcgccc gggcggccac cgctcttgcc
     1561 atagccgcga tagttcacga gcagcaccgc gcggcgcccg tagtggcgct cggcctcggg
     1621 cgcataggcg gtcacttcct ccgcgttgcc gccgaagtag atgaccacgg cccttcgcga
     1681 ggcaggctcg cccggcggaa gcaggagaac gccccgcagc ggcgtggcgt ccgcggcggt
     1741 caaggcaacc tcttcgaggc tccagccggg cggggccgcg gggctcccgc ggaccggctg
     1801 cgggtagaag agaatgcttt cctgcgcgag ccacagcatc gccgccatgc ccgcgtaggc
     1861 aagcacggag gcgacgagaa gggtcttgag cgtggcaggc cccggggggc ggctcagcgc
     1921 ggcaggtccg acgatcccat gaggaacgcg tccacctcgc gcgcgcactg gcggccttcg
     1981 cggatggccc agaccacgag cgactggcca cgccgcatgt cgccggcggc gaagaccttg
     2041 gctaccgagg tcgcgtagca gcccacgcct tcggtgccgg ccttcacgtt gccgcgcgcg
     2101 tccttttcca cgccgaggcc ggccacgagt ccctcatgta ccgggtgcac gtaacccatg
     2161 gcgaggagca cgagttcggc gggaatcgtg aactcggtgc cgggcacctc cttcatcgac
     2221 atgccgccct tctcgtcctt cacccactcg acgtggcagg cggtgaggct cgcgaccttg
     2281 ccgcccttgc cctcgaagcg cttggtggcc acggcgaagt cgcgctcggc gccttcttcg
     2341 tgggacgagg aggtgcgcag cttgagcggc cagtacggcc aggtgagcgg cttgtcctcc
     2401 tgctcggggg gccggggcag gagctcgaag ttcaccaccg acttcgcgcc ctggcggatg
     2461 gaagtgccga tgcagtcgct gcccgtgtcg ccgccgccga tcacgacgac gtgcttgccg
     2521 gtggcgagga tctggcccgg taccttgtcg cccgcgacga ccttgttctg caacggcagg
     2581 aagtccatgg cgaaatggat gccggcaagc tcgcggccgg gcaccggcag gtcgcgcggc
     2641 ttttccgagc cgccggaaag caccacggcg tcgaacccgg cgacgaggtc atcggctttc
     2701 aggtccacgc ccacgttcac gccggtgcgg aaggtgacac cctcggcgcc catctgctcg
     2761 atgcgccggt cgatgaggag cttctccatc ttgaagtccg ggatgccgta gcggaggagt
     2821 cccccgatcc ggtcgctctt ctcgaaaagc gtcacgtcgt ggccggcgcg cgcgagctgc
     2881 tgcgcgcagg cgaggcccgc ggggcccgaa cccaccacgg ccaccgtgcg cccggtcttg
     2941 ttcgccggca cctcgggcac gatccagccc tcggcccagc ccttgtcgac gatggcctgc
     3001 tcgatcgact tgatggcgac cgggtcgtcg ttgatgttga gcgtgcacgc cgcctcgcag
     3061 ggcgccgggc agacgcggcc ggtgaactcc gggaagttgt tcgtggaatg cagcacctcg
     3121 agcgcgctct tccagttgtt ccggtagacg aggtcgttcc agtccgggat cacgttgttg
     3181 accgggcaac cggtctggca gaacgggatg ccgcagtcca tgcaacgcgc gccctgcagg
     3241 ctcatgtccg cgtcggtgag cggtcggatg aactccttgt agaccttgat gcgctcggcg
     3301 acgggtgcgt agccgcgatc cttgcgcgcg atttccatga agccggtgat ctttcccatg
     3361 atgtggttct tctcagtcgt tggcgcggac ttccaccacc ccggccgctt cgccgcggcg
     3421 cgcctgctcc agcgccatct cgccgagggc ccgcttgtac tcgatcggca tcaccttcac
     3481 gaagcgcggc aggtaggccg cccagttctc gaggatgcgc cgggcgcgtt cgctgccggt
     3541 gtagagcagg tggcactgga tgaggctctt gatggttgcc tcgtcgttgc cagccaggtg
     3601 gtccacctgg accttgccgt gcgtctcgag ctccacgttg gcgcccgcgt cggccgccat
     3661 gcggtcctcc tcgggtatcg gctccagctc caccatcgcc aggttgcaac gctgggtgaa
     3721 gtcgccttcc tcgtcgagca cgtaggcgat gccgccggac atgccggcgg cgaagttgcg
     3781 cccggccgtg ccgagcacca ccaccgtgcc gcccgtcatg tattcgcagc cgtggtcgcc
     3841 cacgccttcc accaccgccg tggcgccgga attgcgcacc gcgaaacgct cgccggccac
     3901 gccgcggaag tacgcctcgc cgctgatggc gccgtacatg acggtgttgc cgacgatgat
     3961 gttctcctcg gggacgatgg ggcagtcctt cggggggtag acgacgatgc ggccgccgca
     4021 caggcccttg cccacgtagt cgttggcgtc gccctcgagc tcgaaggtga tgccgcgcgc
     4081 gagccagccg ccgaaggtct ggccggcggt gcccgtgaac ttcacgtgga tggtgtcctc
     4141 gggcaggccg gcgtggccgt ggcggcgcgc gacctcgccg gagagcatgg cgcccaccgt
     4201 gcggttcctg ttgaagatgg cgctttccag ctgcaccggg cgcttcgact cgagcgcggg
     4261 gcgcaccttc tcgatgagct ggtggtccag cgccttttcc agcgcgtggt cctgggcctg
     4321 cgtgcagtgg atgggcacgg aggcgtcgac cgtcgggcgg tggaagagac gcgagaaatc
     4381 gagccccttc gccttccagt gctcgatgcc gcggcgcatg tcgaggaggt cggaacggcc
     4441 gatcatgtcc tcgaaccggc ggaagcccat cttcgccatc cactcgcgca cttcctcggc
     4501 gacgaagaag aagtagttga tgacgtgctc gggcttgccg gtgaacttct tgatgagctc
     4561 cggatcctgc gtggccacgc ccaccgggca ggtgttgagg tggcacttgc gcatcatgat
     4621 gcagccctcg accaccagcg gggcggtggc gaagccgaac tcgtccgcgc cgagcagcgc
     4681 gccgacgacg acgtcgcgcc ccgtcttcat ctggccgtcg gtctgcacgc agattcgcga
     4741 gcgcagcttg ttgagcacca gcgtctgctg cgtttcggcg agcccgatct cccaggggct
     4801 gccggcgtgc ttgatcgacg agagcggcga ggctcccgtg ccgccgtcga agccggagat
     4861 ggtcacatgg tcggccttgg ccttggccac gccagcggcg accgtgccca cgccgatctc
     4921 ggaaaccagc ttcaccgaca cgcgcgcctt cgggttcacg ttcttgaggt cgtggatgag
     4981 ctgcgcgagg tcctcgatcg agtagatgtc gtggtgcggc ggcggc
//
LOCUS       DASUEV010000265         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_754447, whole genome shotgun sequence.
ACCESSION   DASUEV010000265 DASUEV010000000
VERSION     DASUEV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754447"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFV55_08580"
     CDS             complement(<1..234)
                     /locus_tag="VFV55_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944396.1"
                     /translation="MSASAPNGPARPAAPLAAEPLPVPPRDDLVAGPIVRAKLAKLGI
                     HSRFDLVLHLPLRYEDETKLTPLAEAGTGEPVLV"
     gene            complement(231..884)
                     /locus_tag="VFV55_08585"
     CDS             complement(231..884)
                     /locus_tag="VFV55_08585"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEX4944397.1"
                     /translation="MARVAPRLYVLAGVNGAGKSSIGGATFRAFGADYYNPDEAAGAL
                     MAANPGLTQAEANSAAWQQGRRLLEKAIAGQLDFAMETTLGASTIPRLLVEASVQGFE
                     VRMWYVGLASPELHIRRVRARVRKGGHDIPEADIRRRFEHSRLNLVHLMPHLAALRVY
                     DNSAEADPAAGKVPELRLLLHLERGRIAGPADLSGTPTWAKPIVAAAMRLHGGRERT"
     gene            complement(930..1277)
                     /locus_tag="VFV55_08590"
     CDS             complement(930..1277)
                     /locus_tag="VFV55_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX4944398.1"
                     /translation="MSTLTYRNRLGELVALPTVAASRFKNEFGAVFEKAALGGAVAIT
                     KHDSPKAVLISYAEFEALVRARFPDLDDLSAQFDGLLAGMQGTRARKAMATAFDATPA
                     QLGKAAARAPKRR"
     gene            complement(1393..1794)
                     /locus_tag="VFV55_08595"
     CDS             complement(1393..1794)
                     /locus_tag="VFV55_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX4944399.1"
                     /translation="MNIKQTVSTPDAPAAIGTYSQAVRAGDTVYLSGQIPLDPKSMQL
                     VEGFENQVRRVFDNLLAVCRAAGGDFDCVVRVTVYLTDLANFAKVNEVMASYFREPYP
                     ARAAVGVASLPRGAAVEIDAVMHLPTPESGG"
     gene            complement(1791..>3050)
                     /locus_tag="VFV55_08600"
     CDS             complement(1791..>3050)
                     /locus_tag="VFV55_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX4944400.1"
                     /translation="PGKFKDYIAIPKANGYQSLHTTLFGPFGMPVEVQIRTAEMHMVA
                     EAGVASHWLYKSADEHSDVQLKTHQWLQSLLDIQSESGDALEFLEHIKVDLFPEEVYV
                     FTPKGKIFSLPRGATPIDFAYSVHTDVGNRCIAARVNGESVPLGTALRNGDRVEIVTD
                     PDSRPNPVWLSYVATGKARSHIRHYLKTMQSAESAEVGELLLVQALRSLKANPDEIGD
                     EHWKRFFKGDAARTRAEVMADIGLGKRLAVVVAKKLLATPEAVAEGTHRLESVTIRGT
                     EGLAVQFAPCCRPIPGDPIIAQIKKGQGLVVHTHDCPTIHPFHFDPEKWVDVQWDPAP
                     GRLFDVNVRLTASNKRGVLARLAAEISEADSNIDHIETEERASSAYTTIVFTIQVRNR
                     MHLAQVFRRLRRIPEVVRLTRLKKKPA"
BASE COUNT          435 a         1002 c         1097 g          516 t
ORIGIN      
        1 gacgagcacc ggctcgccgg taccggcttc ggcgagcggc gtgagcttcg tctcgtcctc
       61 gtagcgcagc ggcaggtgca gaacgaggtc gaagcgcgag tggatgccga gcttcgcgag
      121 cttcgcgcgg acgatggggc cggcgacgag atcgtcgcgc ggcgggacgg gcagcggctc
      181 ggcggcaagg ggcgccgcgg gtctggccgg tccattcggc gccgacgcgc tcatgtcctt
      241 tcccttcctc cgtgcagccg catcgcggct gccacgatcg gctttgccca cgtgggcgtg
      301 cccgacaggt ccgcgggtcc ggcgatccgg ccgcgttcga gatgaagcaa cagccgcagt
      361 tcgggcacct tgcccgccgc cggatcggct tccgcgctgt tgtcgtagac gcgcagggca
      421 gcgaggtgcg gcatcaggtg cacgaggttc agccggctgt gctcgaatcg gcgccggatg
      481 tcggcttccg ggatgtcgtg gccgcccttg cgcacgcggg cacgcacgcg acggatgtga
      541 agttcgggac tcgcgaggcc cacgtaccac atccgcactt cgaacccctg cacggacgcc
      601 tccacaagca ggcgcgggat ggtgcttgcg cccagcgtgg tctccatggc gaagtcgagt
      661 tgtccggcga tggccttctc gagcaggcgc cgcccctgct gccacgcggc gctgttggct
      721 tccgcttggg tgagaccggg gttcgctgcc atcagcgcgc cggcggcttc gtcgggattg
      781 tagtagtcgg cgccgaaagc ccggaaggtc gcgccaccga tgctgctctt gcctgcgccg
      841 ttgacgccgg cgagcacgta caggcgggga gcgacgcgcg ccacgatcgc ggacccgatg
      901 tcggtgtcat ccggccttgg acgagccggt caacgccgtt tgggcgccct ggcggcggcc
      961 ttgcccagtt gcgcgggcgt ggcgtcgaat gcggttgcca tggctttcct cgccctggtc
     1021 ccttgcatgc ccgcgagcag gccatcgaac tgcgccgaga ggtcatcgag gtccgggaag
     1081 cgagcgcgca ccagcgcctc gaactccgcg taggagatga ggaccgcctt gggcgagtcg
     1141 tgcttggtga tggccaccgc gcccccgagc gccgccttct cgaagacggc accgaactcg
     1201 ttcttgaagc ggctcgcagc gacggtgggc agggcgacca gttcgcccag gcggttgcgg
     1261 taggtgagcg tggacacggg cgcctccggt acgattttgg ccattatagc ccgatggccc
     1321 attttggacg atgaagaggc gttggtatcc cgcggtgcca gacacggttt cagacaccgt
     1381 gtctgacacc catcaaccac cgctttccgg ggtcggcagg tgcatgacgg cgtcgatctc
     1441 gaccgccgcg ccgcgcggca gcgaggccac gcccaccgcc gcgcgcgccg ggtagggctc
     1501 gcggaagtag gaggccatca cttcgttcac cttcgcgaag ttggcgaggt ccgtgaggta
     1561 gaccgtcacc cgcaccacgc agtcgaaatc gcccccggcg gcccggcaga cggcgaggag
     1621 gttgtcgaag acgcggcgca cctggttctc gaagccttcg accagctgca tcgacttcgg
     1681 gtcgagcggg atctgcccgg agaggtacac cgtgtcgccg gcccgcacgg cctgcgaata
     1741 ggtgccgatg gcggcggggg cgtcgggggt cgagacggtc tgcttgatgt tcatgcgggc
     1801 ttcttcttca ggcgagtgag tcggacgacc tcgggaatgc ggcgcaggcg ccggaacacc
     1861 tgcgcgaggt gcatgcggtt cctcacctgg atggtgaaga cgatcgtggt ataggcgctg
     1921 ctcgcgcgct cctcggtctc gatgtggtcg atgttcgagt ccgcctccga gatctcggcc
     1981 gccagccgcg ccagcacgcc gcgcttgttg gaggcggtga ggcgcacgtt cacgtcgaag
     2041 agccgccccg gggccgggtc ccactgcacg tcgacccact tctccgggtc gaagtggaag
     2101 gggtggatcg tcgggcagtc gtgggtgtgc accacgagcc cctgcccctt cttgatctgc
     2161 gcgatgatcg ggtcgccggg aatgggccgg cagcacggcg cgaactgcac cgccaggccc
     2221 tcggtgccgc ggatggtgac ggattcgagg cggtgcgtgc cctcggccac cgcctccggc
     2281 gtggccagga gcttcttggc caccaccacc gcgaggcgct tgcccagccc gatgtcggcc
     2341 atcacctcgg cgcgcgtgcg cgcggcgtcg cccttgaaga agcgcttcca gtgctcgtcg
     2401 ccgatctcgt ccgggttggc ctttagcgag cgcagcgcct ggaccagcag cagctcgccc
     2461 acttccgccg attccgcgga ctgcatcgtc ttcaggtaat gccggatgtg cgagcgcgcc
     2521 ttgcccgtgg cgacgtagga gagccacacc ggattgggcc gcgagtcggg gtcggtcacg
     2581 atctccacgc ggtcgccgtt gcgcagcgcc gtgccgagcg ggaccgactc gccgttcacg
     2641 cgggccgcga tgcaccggtt gcccacgtcg gtgtgcaccg aataggcgaa gtcgatgggg
     2701 gtggcgccgc gcggcagcga gaagatcttg cccttgggcg tgaagacgta gacctcctcg
     2761 gggaagaggt ccaccttgat gtgctccagg aactcgagcg cgtcgcccga ttcgctctgg
     2821 atgtcgagca gcgactgcag ccactggtgg gtcttcagct gcacgtcgga atgctcgtcg
     2881 gcgctcttgt agagccagtg cgaggccacg ccggcctcgg ccaccatgtg catctcggcg
     2941 gtgcggatct gcacctcgac gggcatcccg aacgggccga agagcgtggt gtgcagcgac
     3001 tggtagccgt tcgccttggg gatggcgatg tagtccttga acttgccggg
//
LOCUS       DASUEV010000266         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_407458, whole genome shotgun sequence.
ACCESSION   DASUEV010000266 DASUEV010000000
VERSION     DASUEV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407458"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..509
                     /locus_tag="VFV55_08605"
     CDS             168..509
                     /locus_tag="VFV55_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4944401.1"
                     /translation="MSRNRILIAAILAALAASAHAAGDPAAGKQKNFQCMGCHGIPGW
                     KTAFPEVYQVPKLGGQHAAYIVSALKQYKAGERDHATMRSIASNLSEKDMEDLAAYYS
                     QAAGSAAVAGK"
     gene            518..841
                     /locus_tag="VFV55_08610"
     CDS             518..841
                     /locus_tag="VFV55_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4944402.1"
                     /translation="MKMHTLAALAALALSAPAFAAKGDPEAGKTKAQPCRACHGEAGI
                     SAVPDFPKIAGQHADYIATVLRHYKVGKRKNPIMAGQVANLSENDMLDLAAYFSTQSG
                     LAIKY"
     gene            complement(897..1331)
                     /locus_tag="VFV55_08615"
     CDS             complement(897..1331)
                     /locus_tag="VFV55_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEX4944403.1"
                     /translation="MSLFNPTRDQVREFFFGLWEKSQAGAPLTPLETMALAIVNEHRE
                     YHEVLANPDRYRDREWKPEGGETNPFLHLSMHLAIEEQVSIDQPPGIREAVRKLAAKH
                     DSEHDARHAVMECLAEIIWQTQRNAAGFDNAAYLRCLTLKGG"
     gene            complement(1328..2002)
                     /gene="nth"
                     /locus_tag="VFV55_08620"
     CDS             complement(1328..2002)
                     /gene="nth"
                     /locus_tag="VFV55_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX4944404.1"
                     /translation="MKPEARVDFFRILREANPEPKSELEYATAYQLLVAVVLSAQATD
                     KGVNLATRKLFPVAGTPEKMLALGEEGLREYIKTIGLFRTKAKNVIGLSKKLVEEHGG
                     EVPGTREALEKLPGVGRKTANVVLNVAFGQPTLAVDTHIFRVANRTGLAPGKDPLAVE
                     LKLLKVVPAEFRLHAHHWLILHGRYTCLARSPKCGQCAVYAPCGFKDKARLAAPPAPK
                     RSRLPK"
     gene            complement(2002..2511)
                     /locus_tag="VFV55_08625"
     CDS             complement(2002..2511)
                     /locus_tag="VFV55_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HEX4944405.1"
                     /translation="MSELADRIDALLPQTQCTKCGYDGCRPYAEAIAKGEADIDQCPP
                     GGDEGIAKLAALLDRTVKPLNPVHGEYRPPQAAFIDESLCIGCVKCIHACPVDAIVGA
                     SKLMHTVIAGWCTGCELCIPPCPVDCISLVPVARLPDPELSRERFAFRAFRLARDEAE
                     RTARLEAYK"
     gene            complement(2508..>2806)
                     /locus_tag="VFV55_08630"
     CDS             complement(2508..>2806)
                     /locus_tag="VFV55_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX4944406.1"
                     /translation="SETSRPTTHASPTYVEEGVVHYCVANMPSAVARAGAAALSAAAL
                     PYVRALAGKGIARAIREDEGLRAGVLLWRGRFTHQGIASEAGRPFSPLADEDLA"
BASE COUNT          455 a          959 c          928 g          464 t
ORIGIN      
        1 ggcttccacc tggggcctcc gcgccccctg gacgccacct tcgccgagga cccttgagcg
       61 ccgtgacgca ggggcgggtc gtgggctata atccgcgccg aatcggcgcc gggccttggg
      121 aagaggatcc gccgcctctc ccgaagtcca gaccataacg agacgccatg tcccgaaatc
      181 gcatcctcat cgccgccatc ctcgcggcgc tcgccgcctc ggcccacgcg gccggagacc
      241 ccgccgccgg caagcagaag aatttccagt gcatgggctg ccacggcatc cccggctgga
      301 agaccgcttt ccccgaggtc taccaggtac ccaagctggg cgggcagcac gccgcctaca
      361 tcgtttcggc gctcaagcag tacaaggcgg gcgaacgcga ccacgcgacg atgcgttcca
      421 tcgcctcgaa cctctccgag aaggacatgg aggatctcgc ggcgtactac tcgcaggcgg
      481 ccggcagcgc ggccgtcgcc gggaaatgag gcgcgccatg aaaatgcaca ccctcgccgc
      541 gctcgccgcc ctggcccttt ccgccccggc tttcgcggcc aagggcgatc ccgaagccgg
      601 caagaccaag gcgcagccct gcagggcctg ccatggcgag gccggcatca gtgccgttcc
      661 ggacttcccc aagattgccg gccagcatgc cgactacatc gccaccgtgc tcaggcacta
      721 caaggtcggc aagcgcaaga acccgatcat ggccggccag gtcgcgaacc tctccgaaaa
      781 cgacatgctc gacctcgctg cgtacttctc gacccagtcg ggcctggcga tcaagtactg
      841 acgctctgta ccgggtacat gtacccggta cattttcagg tctgaagcca agggcttcag
      901 ccccctttca gtgtcagaca cctcagatag gcagcgttgt cgaagccggc ggcgttgcgc
      961 tgtgtctgcc agatgatctc ggcgaggcac tccattacgg cgtgacgcgc atcgtgctcg
     1021 gagtcgtgct ttgccgccag cttccgcacg gcctcccgga tgcccggcgg ctggtcgatg
     1081 gagacctgct cctcgatggc gaggtgcatg gacaggtgca ggaacggatt cgtctcgcct
     1141 ccctcgggct tccattcccg gtcgcgatag cggtcgggat tcgcgagcac ctcgtggtac
     1201 tcccgatgct cgttcacgat cgcgagggcc atcgtctcca ggggcgtgag aggcgcgccc
     1261 gcctgcgatt tctcccacag gccgaagaag aactcgcgca cctggtcgcg ggtgggattg
     1321 aaaaggctca tttcggcagg cgcgaccgtt tcggggccgg gggggcggcg agcctcgcct
     1381 tgtccttgaa cccgcagggc gcgtacaccg cgcattggcc gcacttcggc gagcgtgcga
     1441 ggcaggtgta gcggccgtgc aggatcagcc agtggtgcgc gtgcaggcgg aattccgccg
     1501 gcaccacctt gaggagcttc agttccaccg cgagggggtc cttgcccggc gcgaggccgg
     1561 tgcggttggc gacgcggaag atatgcgtgt ccacggccag ggtgggttgg ccgaaagcga
     1621 cgttcaggac cacgttggcc gtctttcgcc ctacgccagg cagtttctcc agcgcctcgc
     1681 gcgtgcccgg cacctcgccg ccatgctcct cgaccagctt cttcgacagg ccgatcacgt
     1741 tcttcgcctt cgtgcggaac aggccgatcg tcttgatgta ttcgcgcagc ccttcctccc
     1801 cgagcgccag catcttctcc ggcgtgccgg ccacgggaaa gagcttgcgc gtggcaaggt
     1861 tcacgccctt gtcggtggcc tgcgccgaga gcacgaccgc caccagcagt tggtaggctg
     1921 tcgcgtactc gagctcgctt ttcggctcgg gattggcctc gcgcaggatg cggaagaaat
     1981 cgacgcgcgc ttcgggcttc atcatttgta ggcctcgagc cttgccgtgc gttcggcctc
     2041 gtcacgggcg agacggaagg cgcggaacgc gaaacgttcg cgcgagagtt ccgggtcggg
     2101 cagtctcgcc acgggcacca gggagatgca gtccacgggg cagggcggga tgcacagctc
     2161 gcaacccgtg caccagccgg cgatcaccgt atgcattaac ttggaggcgc ccacgatggc
     2221 atccacagga caggcgtgga tgcacttcac gcagccgatg cagagcgact cgtcgatgaa
     2281 ggcggcctgc ggcgggcggt actcgccgtg aacaggattg aggggtttga ctgtgcgatc
     2341 gagcagggcc gcgagtttcg cgatgccttc atcgcccccc ggcgggcact ggtcgatgtc
     2401 cgcctcgccc ttcgcgatcg cctcggcata gggacggcag ccgtcatagc cgcacttggt
     2461 gcactgcgtt tgcgggagca gcgcgtcgat gcggtcggcg agctcgctca cgcgagatcc
     2521 tcgtccgcga gtggggaaaa aggccgtccc gcttcggagg cgatgccctg gtgcgtgaac
     2581 cggccgcgcc acagcaggac acccgcgcgc aagccttcgt cctcgcggat cgcccgcgcg
     2641 atgcccttgc ccgccagcgc ccggacatag ggcaaggccg ccgccgaaag cgccgcggcg
     2701 ccggcgcgcg ccacggccga gggcatattg gccacgcagt aatgcacgac gccttcctcg
     2761 acgtaggtgg ggctcgcgtg cgtggtcggg cgcgacgtct cggaca
//
LOCUS       DASUEV010000267         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_407497, whole genome shotgun sequence.
ACCESSION   DASUEV010000267 DASUEV010000000
VERSION     DASUEV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407497"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1061
                     /locus_tag="VFV55_08635"
     CDS             141..1061
                     /locus_tag="VFV55_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX4944407.1"
                     /translation="MQFRRNDFDVSNRINTTDPVCVRLEITRLHRSSHPGIQAPALAR
                     AFDDVTALYRGEDPSYEKCDTAYHDLQHVLEVALAMARLLDGYERSRGDGPAIGERLF
                     QLGIVCALFHDVGYLRRRADRRHRRGAEYTRTHVSRGARFLRKYLPTIGLGEFADVAG
                     PVLHFTGYERAVSAIRLPHPVYRLLGSLLGSADIIAQMSDRCYLEKCHDRLYPEFVDG
                     GIARRVTGPDVETVFASADDLVRKTPGFFVGASRRLDHDLGGAYQYAGNHFGGANLYM
                     EAVKQNIRFAEKLRDGTSLLLRRIPPVTIN"
     gene            complement(1077..2042)
                     /locus_tag="VFV55_08640"
     CDS             complement(1077..2042)
                     /locus_tag="VFV55_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944408.1"
                     /translation="MQRFIRHAAAIVATMLALGANAQGYPNRPVKIVVPFSPGGFTDV
                     VARILQTQLGAALGQPVVIENKPGAGSTIGTAEVANARPDGYTLAMVSTTHVISPSLY
                     RKMPYDALADFTPVMKLAEGPYVLVVHPSLPVRNVKELIALARAEPGKIDYASSGNGS
                     SQHLVGALFGQMAGVTLNHVPYKGSGQAMQDLVGGQVKVSFVGAPNAVPYLASGKLRA
                     LGVTTKKRSADLPDIPSIDEAGVAGYDATLWLGLLAPPGTPADVVARLREGVTKALST
                     PEARKLVNSAGVDVAFSTPEEFAALMKSELARWGKVVKETGATVN"
     gene            complement(2090..3007)
                     /locus_tag="VFV55_08645"
     CDS             complement(2090..3007)
                     /locus_tag="VFV55_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX4944409.1"
                     /translation="MPDTTTWEGGTEHWTTKGDVRLFLWNKKPAPGTTPAGTILFVHG
                     SSMASQPTFDLYVPGRPDSSVMDWFAARGYDTWCMDNEGYGRSDKLRPINCDISNGAD
                     DLAAGSGYILEATGAKGLLVYGISSGALKAGLFAQRHPERVARLALDAFVWTGDGSPT
                     LAERRKKLAQFQSMNRRPMDRAFVRSIFSRDHPGTADEATIEAFADAILALDDSVPTG
                     TYVDMCSKLPLVDPEKLTVPVIVMRGEYDGIAGFDDLVEFWKRLPNADKNFTVMAGIS
                     HASFQQKNYRMVYHILHAFFAQPAPAYRA"
BASE COUNT          493 a         1052 c         1086 g          543 t
ORIGIN      
        1 cccaagcctg tccgttggct agtcttgaca ggagcgggaa taataacggg acagggggtg
       61 ttgacacgcg agatgcccga atcttccccg gcgaaggggc gggaaaaaac aacaggcggg
      121 acacccaggc gaggcgcacg atgcagttcc gtcgcaacga tttcgacgtg agcaaccgga
      181 tcaacacgac cgatccggtc tgcgtgcggc tggaaatcac gcgccttcac cgctccagcc
      241 atcccgggat ccaggcgccc gcgctcgcgc gcgccttcga cgacgtgacc gccctctatc
      301 ggggcgagga tccgtcctac gagaagtgcg acacggccta ccacgacctc cagcacgtac
      361 tcgaggtggc gctggcgatg gcgcgccttc tcgacggcta cgaacgctcg cgcggcgacg
      421 ggccggcgat cggcgagcgg ctgttccagc tgggcatcgt ttgcgcgctc ttccacgacg
      481 tgggctatct tcgccgccgg gccgaccgtc gtcaccgccg cggcgcggag tacacgcgca
      541 cgcatgtgtc gcgcggggcg cggttcctgc gcaagtacct gccgaccatc ggcctcgggg
      601 agtttgcgga cgtcgcgggc ccggtgcttc acttcaccgg ctacgagcgg gcggtttccg
      661 ccatacgcct gccgcatccg gtttaccgcc tgctgggcag cctgctcggc tcggccgaca
      721 tcattgcgca gatgtcggac cgctgctacc tcgagaaatg ccatgatcgc ctctacccgg
      781 aattcgtcga cggcggcatt gcgcggcgcg taaccggccc cgatgtcgag accgtcttcg
      841 cctcggccga cgacctcgtg cgcaagacgc ccggattctt cgtcggcgcc tccaggcgcc
      901 tcgaccacga cctggggggc gcgtaccagt acgccggcaa ccacttcggc ggcgcgaacc
      961 tctacatgga ggccgtgaag caaaacatcc gcttcgccga aaagcttcgc gacggaactt
     1021 cgctcctcct gcgccggatt ccgcccgtca ccatcaactg aaggcggctc gcgccttcag
     1081 ttcacggtcg cgcccgtctc cttcacgacc tttccccacc gcgccagctc cgacttcatc
     1141 agcgcggcga actcctcggg tgtggaaaat gccacgtcga ctccggccga attcacgagc
     1201 ttcctggcct cgggcgtgct gagcgccttc gtcacgcctt cgcgcagcct ggccaccacg
     1261 tcggccggcg ttcccggcgg ggcgagcaac cccagccaga gcgtcgcgtc gtagccggcc
     1321 acgccagcct cgtcgatgga ggggatgtcg ggaaggtccg ccgagcgctt cttcgtcgtg
     1381 acaccgagcg cgcgcagctt cccgcttgcg aggtagggca ccgcgttggg cgcgccgacg
     1441 aagctcacct tcacctggcc gccgacgagg tcctgcatcg cctggccgct gcccttgtag
     1501 ggcacgtggt tgagcgtcac cccggccatc tgcccgaaga gcgcgcccac gaggtgctgg
     1561 ctgctgccgt tgccggacga ggcatagtcg atcttcccgg gctccgccct ggcaagcgca
     1621 atgagttcct tcacgttcct gaccggtagg ctgggatgca cgacgaggac gtaaggcccc
     1681 tcggcgagtt tcatcaccgg cgtgaagtcg gcgagcgcgt cgtagggcat cttcctgtag
     1741 agcgaggggc tgatcacgtg ggtggtggac accatcgcca gcgtgtagcc gtcgggcctg
     1801 gcattcgcga cctccgcggt gccgatggtg gagcctgctc cgggcttgtt ctcgatgacc
     1861 acgggctgcc cgagcgcagc gccgagttgc gtctgcagga tgcgcgcgac gacgtcggtg
     1921 aagccgcccg gggagaatgg cacgacgatc ttcacggggc ggttggggta gccctgggcg
     1981 ttggctccga gggcgagcat cgtggcgaca atggccgcgg catggcggat gaagcgctgc
     2041 atggggtctc ctctcgtctt gtcttgtcgt cgtcgcgccc acggcgcgtt caggcccgat
     2101 aggcgggcgc cggctgggcg aagaaggcgt gcaggatgtg atagaccatc cggtaattct
     2161 tctgctggaa gctcgcgtgc gagatccccg ccatcacggt gaagttcttg tcggcgttgg
     2221 gcaggcgctt ccagaactcc accaggtcgt cgaaaccggc gatgccgtcg tattcgccac
     2281 gcatcacgat gacgggtacg gtgagcttct ccggatccac gagcggcagc ttcgagcaca
     2341 tgtcgacgta ggtgccggtg ggcacggaat cgtcgagggc gaggatcgcg tcggcgaacg
     2401 cctcgatggt cgcctcgtcg gccgtgcccg gatggtcgcg gctgaagatg ctgcgcacga
     2461 acgcacggtc catcgggcgg cggttcatcg actggaactg cgcgagcttc ttgcggcgct
     2521 cggccagcgt ggggctgccg tcgcccgtcc agacgaaggc gtcgagggcc aggcgcgcga
     2581 cccgctcggg atggcgctgg gcgaagagcc cggccttgag ggcgcccgag gagattccgt
     2641 agacaaggag gccctttgcg cccgtcgcct caaggatgta accggagcct gccgcgaggt
     2701 cgtcggcgcc attcgagatg tcgcagttga tcgggcgcag cttgtccgag cggccgtagc
     2761 cctcgttgtc catgcaccac gtgtcgtagc cgcgtgcggc gaaccagtcc atcacggaag
     2821 agtcgggacg gccgggcacg tagaggtcga aggtcggctg cgaggccatg gacgaaccgt
     2881 ggacgaagag gatcgtgccc gccggtgtcg tgcccggtgc gggcttcttg ttccagagga
     2941 agagccggac gtcgcccttc gtcgtccagt gctcggttcc tccctcccac gtcgtggtgt
     3001 cgggcattgc ggccgctccc ctgatgatgt cgcgcgtcgt cgagcggcga ttcataaggg
     3061 atttggcggc gggacgcaat gacgccggtg gggcaagggc gcggctcgat tcgcaaagcc
     3121 gtccggacga tccggatagc acggttcgcg tgcgataatt ccgcccggca gcgc
//
LOCUS       DASUEV010000268         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_174211, whole genome shotgun sequence.
ACCESSION   DASUEV010000268 DASUEV010000000
VERSION     DASUEV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174211"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..1335)
                     /locus_tag="VFV55_08650"
     CDS             complement(454..1335)
                     /locus_tag="VFV55_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4944410.1"
                     /translation="MNAPAAPAPTRVETLAFLCLLAGGCAIAFAPIFVRLSDAGPVAS
                     AFWRTAIAAPILWAWAWRADPKPAPVSAGPLAAAGIFFACDLGVWHWSITYTSVANST
                     LLANLAPIFVTLAGWVLYRRSVTRVFLVGMVTAIAGMFILVGPNFAIGGTRLAGDALG
                     ALTAVFYAGYMLAVKVARDAGASTARLMAWSTTITAIALLPVALLSPQPMLPQAASGW
                     LVLLGLALVSQIFGQGIIAYAFAHLPASLSSVSLLIQPVMAALFAWALFGEAIGAAQF
                     VGGAVVLAGIWLAKRGS"
     gene            1408..2004
                     /locus_tag="VFV55_08655"
     CDS             1408..2004
                     /locus_tag="VFV55_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX4944411.1"
                     /translation="MNNEFLSAVILLTLVTDPFGNMPLVNILLGGVAESRRRVVVVRE
                     CLIAYGLLLAFMFGGHSFLALMHLSQTSLSIAGGVILFMIAIRMVFAKLDGSFGGTAG
                     EEPFIVPMAIPLIAGPSALATVMLMASRDPGRVGMWAAAITVTMAITTVVLLAGSQLY
                     RWLGDHAMQAIERLMGLILTAIAVEMLLAGIREFVKGL"
     gene            2001..2609
                     /gene="greB"
                     /locus_tag="VFV55_08660"
     CDS             2001..2609
                     /gene="greB"
                     /locus_tag="VFV55_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX4944412.1"
                     /translation="MSKAFTKENDGADDLDDLEEAIPIPAGAKNYMTPHGWRRMRDEL
                     AHLVKVDRPEVTNVVSWAARNGDRSENADYQYGKKRLREIDRRIRYLTKRLENAEVVD
                     PATREETDQVFFAATVTYANAKGEETTVRIVGLDETDLGKGFISWISPVARALIKARE
                     GDSVSLVTPGGREELEILAVRYEEIPMPPFVSATSAWSPNAK"
BASE COUNT          492 a          926 c          950 g          413 t
ORIGIN      
        1 cagtccgcca catgacacgg cggaagaggt aacccaaccg acttgtggat gcagtgcatc
       61 gaacccgctc cactgcaaat cactatgcac gaatctccct tgcgcccgtt gactggcgtc
      121 aactgtagat tacgaaagtc ctcgcaccgc aggaagtgct gacccgctgc ccttccatcg
      181 catcggcttc attggtttcc gtgttgtatg ttgttgtcgt cgctgttcgt gacggcgaga
      241 tcgaggacct cgtccccgag ggccatggtg accggcgtct tccgccggga atcgcaccgc
      301 ctcgggcgcg tggacgtgct cgcggccgcc tctgagcgca acgaactgct cgagacctaa
      361 ctgatgcggg agttcctcgg ggcaccgctg gcgcggcgcg gaatctgaaa gcgaaagtgg
      421 gacgaaggtg ggacggttct gtagtactgt ccttcaactt ccgcgcttcg cgagccagat
      481 ccccgcgagc accaccgcgc cgcccacgaa ctgcgccgcg ccgatcgcct cgccgaagag
      541 cgcccacgcg aagagcgcgg ccatcaccgg ctggatgagg aggctgaccg acgacagcga
      601 agcgggcagg tgggcgaagg cgtacgcgat gattccctgc ccgaagatct gcgagacgag
      661 cgccaggccg aggagcacca gccatccgct ggctgcctgc gggagcatcg gctgcggcga
      721 gaggagggcc acgggcagga gcgcgatggc ggtgatggtc gtgctccagg ccatgagccg
      781 cgcggtcgag gcgcccgcat cccgcgccac tttgacagcg agcatgtagc ccgcatagaa
      841 cacggcggtg agcgcgccca gcgcgtcgcc ggcgagccgc gtgccgccga tggcgaagtt
      901 cgggcccacc aggatgaaca tgcccgcaat cgccgtgacc atgccgacga ggaagacacg
      961 cgtgaccgac cgccggtaga gcacccagcc cgccagcgtg acgaagatcg gcgcgagatt
     1021 ggcgagcagc gtcgagttgg ccaccgaggt gtacgtgatc gaccagtgcc acacgcccag
     1081 gtcgcaggcg aagaagatgc ctgcggcggc gagggggccg gcggaaaccg gtgccggctt
     1141 cgggtccgcg cgccatgccc acgcccacag gatcggcgcc gcgatcgccg tgcgccagaa
     1201 ggcgctcgcc acgggcccgg catccgagag ccggacgaaa atcggcgcga aggcgatggc
     1261 gcacccgccc gcgagcaggc acaggaaggc gagcgtttcg acacgggtgg gtgcgggcgc
     1321 ggccggggcg ttcacggcgc cattttcgca cgcggggcgg cggcgacccc cgtcgcgtag
     1381 aatgaccggc cccggatcgt gatcgcgatg aacaacgaat tcctctctgc ggtgatcctg
     1441 ctgacgctgg tcaccgaccc gttcggcaac atgccgctgg tgaacatctt gctgggcggc
     1501 gtggccgaat cgcgccggcg cgtggtcgtc gttcgcgagt gcctcatcgc ctacggcctg
     1561 ctgctcgcct tcatgttcgg cgggcattcc ttcctcgcgc tcatgcacct gtcgcagacc
     1621 tcgctgtcga ttgccggcgg cgtgatcctg ttcatgatcg ccatccgcat ggtgttcgcg
     1681 aagctggacg ggtccttcgg cggcacggcc ggggaggaac ctttcatcgt cccgatggcc
     1741 ataccgctta tcgccggacc ctcggcgctc gcgaccgtga tgctcatggc gtcgcgcgac
     1801 cccggccgcg tgggcatgtg ggcggccgcg atcaccgtga cgatggcgat cacgaccgtg
     1861 gtgctcctgg ccggatcgca gctgtaccgc tggctgggcg accacgccat gcaggccatc
     1921 gagcggctga tggggctcat cctcaccgcg atcgcggtgg agatgctgct tgccggcata
     1981 agggagttcg tgaagggcct atgagcaagg cattcaccaa ggaaaacgac ggtgccgacg
     2041 acctcgacga cctcgaggaa gcgataccca ttcccgccgg cgccaagaac tacatgacgc
     2101 cccacggctg gcggcgcatg cgcgacgagc tggcccacct cgtcaaggtg gaccggcccg
     2161 aagtcacgaa cgtcgtctcc tgggccgcgc gcaacggcga ccgctccgag aacgccgact
     2221 accagtacgg caagaagcgg ctgcgcgaga tcgaccggcg catccgctac ctcaccaagc
     2281 ggctcgagaa cgcggaggtc gtggaccccg ccacccgcga ggaaaccgac caggtcttct
     2341 tcgcggccac cgtcacctat gccaacgcga agggcgagga gacgacggtg cgcatcgtgg
     2401 ggctggacga gacggacctc ggaaaaggct tcatcagctg gatctcgccc gtggcccgcg
     2461 cgctcatcaa ggcgcgcgag ggcgactcgg tgtcgctggt cacgcccggc ggccgcgagg
     2521 aactggagat ccttgcggtt cgctacgagg agattcctat gccgcccttc gtctccgcga
     2581 cgagcgcctg gtcgccgaac gcgaagtagc cgccggcggc gggtgaagtc gaagaggcgg
     2641 cggcggtgga agtcgatcgc aatcctgggc acgcaagcga gaccgtcgga tgggtgcccg
     2701 gaaagtcgag gccaggcaga acaagccagg cctcgacttt ccacccatct aatgacgagg
     2761 gcgctgcgct gcctcgtccg c
//
LOCUS       DASUEV010000269        12793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_884384, whole genome shotgun sequence.
ACCESSION   DASUEV010000269 DASUEV010000000
VERSION     DASUEV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884384"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFV55_08665"
     CDS             complement(<1..425)
                     /locus_tag="VFV55_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944413.1"
                     /translation="MAAAPRHRSGEHAARRYSRREFLSNTGAAGAAIIMPGLVPLATS
                     AAPTSVPGTARLLPAPATEGGMPLMQALSQRCSTREFSPRKLPDQVLSDLLWAADGIN
                     RPGGDRTAPCWRHIMVIDVYAAMEDGLWLYDPKRHALIA"
     gene            complement(582..2297)
                     /locus_tag="VFV55_08670"
     CDS             complement(582..2297)
                     /locus_tag="VFV55_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX4944414.1"
                     /translation="MFRTKYLDYAELFAQLAAWSGAHPDIVRVTSIGKSAEGRDIPLV
                     TIGRDPDAARPAVWIDGNMHASELCGSSVALALAEDIVAIHQGKDEAGGKPLPKHMAD
                     AIRQALFYVVPRISPDGAEAVLKTGRYVRSSPVNDRVPRDHAYWEAADIDGDGVMGFM
                     RQECPDGELVELRGEDGNPLNPPVMVPRGPEDAGPCYRLYPEGRIVNFDGRRIPDPYF
                     LSDNAYDFNRNFPYQWAAEPKQEGAGHFPGSAPETRAIMEFATKHPHIFAWLNLHTFG
                     GVLIRPLGDQPDHKMDQSDLALFRQVEAWMTEHTGYATVSGYHEFLYEPEKPLFGDIT
                     EYAYHQRGCIAYVVELWDLFRQLGIERKKPFVDHYTRLDRKDYLALARFDREQNQGRI
                     FSRWRKARHPQLGEVEVGGFDARVGISNPPYEKLAETCAAQSAAFLRVAALVPQVSLE
                     VVKQEKAGADHTRIEVRVANSGYLGTYGLTSAKKLPISEPLRLPTEGAGVKLVAPAES
                     IVEIGHLDGWGSGLYAGSNIFFPWTRGNVNEKFVTLVAQGRGKLTLKVGSCRVGYRSL
                     EIDVA"
     gene            complement(2334..2732)
                     /locus_tag="VFV55_08675"
     CDS             complement(2334..2732)
                     /locus_tag="VFV55_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX4944415.1"
                     /translation="MPGRFGIDTSILVRLATGDPEREFDMCVRKLSALVKGDSAEVFA
                     SNQVIGETYIALQHHYGVPKAEARAALASVLRSGLVSPFSGAGVFASLETHAGCGLLD
                     RLIADDYRRAGLVTLTLDRRMAALPQARHL"
     gene            complement(2710..2952)
                     /locus_tag="VFV55_08680"
     CDS             complement(2710..2952)
                     /locus_tag="VFV55_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX4944416.1"
                     /translation="MFVKITSKRQVTFPARVLEALGAKAGDRLELSESPEGYVLRACA
                     IDRTRLAPLRGKLRRGQGTFDLETFREKPHARSLRN"
     gene            complement(3090..6449)
                     /locus_tag="VFV55_08685"
     CDS             complement(3090..6449)
                     /locus_tag="VFV55_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX4944417.1"
                     /translation="MQDRDSHSKPIEHLVAHFETHLERGITQQDALDRLARHGANELT
                     ERPRPGFLALLWDQFNNYLVIILVIAAVVSLALGEWVDSIAIMVIVVLNAVVGVIQES
                     KAEQALAALKKMAAPSAQVIRDGHQVTIPGREIVGGDIVLLEAGNYVPADVRLVESHN
                     LKVEEASLTGESVPVDKNPAVVLDKEIPLGDRKNTAFMGTLITYGRGRGLVTCTGMNT
                     QIGLIAQMIQSFEDEATPLQKKLEHLGKVLGTACLAICAIVFVYGLFRDTHIADVANI
                     GLLAYLEAERKDIINLFMTAVSLAIAAVPEGLPAIVTICLALGMQRMIKHHALIRKLP
                     AVETLGCATVICSDKTGTLTQNQMTVVQAWAGGRRFRITGEGYNPTGEFFLGEERFDP
                     RTNPDATALLQGAIACNDAMLEERPDESGARSWNIIGDPTEGAMVVAAAKSGYLQDEF
                     AKSMPRVQEIPFDSERKRMTTIHRNDASGTAQPGFDFPPLVAFVKGAPDIVLELCGSM
                     LDGGRAVELTHAMRSSILDQNRDMASDALRVLGVAYRALDEIPATVSPETVEKNLVFV
                     GLLGMIDPPRPEVIAALKVARGAGLKTVMVTGDYKDTAEAIARDIGLRTPDGLVLTGA
                     EIDKLSDEELAARADRLEVCCRVSPQHKTRIVEAFKARDHVVAMTGDGVNDAPALKRA
                     NIGVAMGITGTDVAKQTADMVLTDDNFASIVAAIEQGRIIYSNIRKFVYFLLACNVGE
                     ILIVFGAMLFGMPMPLRPVQLLWLNLVSDGAPALALGLEKGDNDIMTQPARSPKEPVI
                     NRDMAVGIGVVGVVDMIAILAVFYLAMQRYPDHLEAAQTIAFVTLCTSELIRAFAARS
                     EYHSVFSIGVFSNRWMVAAVGVSFVLVLAVVYVPFLQPFFDTVPLSGADWLLMLPFFF
                     ASPVAMELLKIYFRRRHAPVRVKALLPVPQPVTGGNIMLKVLVPVDGSGNCKFAVQHV
                     IKQFMNNTAMEVHLLNVQAPFSADIAHFTSRMSRQDYHREQGEKALQPVRQMLDNFSI
                     PYSMHIEVGDRAKVIADEARRLRCDQIVMATARKNSLTRLVESSVTNEVLELATVPVE
                     LIAGDSMSKWERYGIPAAIAAVLALVLVVED"
     gene            6905..7852
                     /locus_tag="VFV55_08690"
     CDS             6905..7852
                     /locus_tag="VFV55_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4944418.1"
                     /translation="MLQPNIHRYLRHGMLPQLRVFEASARLGSFTRAAQELHMAQPTA
                     SVQLKKLSETVGVPLFEQVGKRIYLTDAGRRLYLGCQEVFRAFSAIEESLAGMRTLEA
                     GRLRLGVSTTGMCFAPRLLGAFVGLHPGVEISMQAHNRQTLVERLAGNEDDLYLFADA
                     PELEDVVAQELLPNPLVVLARDDHPLAAEKNIAFERLAREPFLVREEGSGARRIMMRL
                     FHQHGLVPKVRMELGTNEAIKEAVLAGLGIAIMSRYTFGLDPESSRYVCLDVAGFPIE
                     NHWYFVYPAGKQLTAAARAFLDFARTQAKGLVRESLARH"
     gene            7992..8447
                     /locus_tag="VFV55_08695"
     CDS             7992..8447
                     /locus_tag="VFV55_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944419.1"
                     /translation="MTFSTGASAGDPLAGALIGGGIGAAVAGPPGAAVGAIIGTIAGS
                     THPYYYGERGYYDRGYYDRDTYAPEPAYYAPPPAYYAPAPVYYAPARVYYAPPPVYYG
                     PSVAYRAAPAYVYGTRHATYDRRGDRHRDGYRDGHDGGRYRDGYDRRFR"
     gene            8613..9650
                     /locus_tag="VFV55_08700"
     CDS             8613..9650
                     /locus_tag="VFV55_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX4944420.1"
                     /translation="MKTTTRKPIALAGLVAAALLAAVPAHAGKTLDAIKARGQLVCGV
                     HTGLAGFSQADTQGNWSGLDVDVCRAIAAAVLGDGSKVKFVPLTAQQRFTALQSGEID
                     ILSRNTTWTLSRDASLGLHFIGTTYYDGQGFMVPVKTKIKSAKQMKGATVCVQSGTTT
                     EKNMTDFSRANKLDLKPVVFEKIEAATGAYFSGRCAAFTTDASGLASVRNKEAKNPAE
                     HVILPELISKEPLGPAVRRGDDEWFAIAKWVVFGLLESEEYGITQANVEKMTNESQDP
                     VVMRITGKSEDMGKLLGLERDWMVKAVKAVGNYGEIFDRNVGPKTPLGLPRGINAQWN
                     KGGLMYAPPIR"
     gene            9667..10845
                     /locus_tag="VFV55_08705"
     CDS             9667..10845
                     /locus_tag="VFV55_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX4944421.1"
                     /translation="MSGHPPPRRRDWSWRSQAFRGLVYQVLAAAVIGLALWFLAHNTV
                     ENMRVRGIQSGFGFLVQPAGFDIGESLIPYDALDRNWRAFLVGVLNTLRVAIVGVVLA
                     TVLGALLGVGRFSRNAIVRGLCYAYVELFRNIPVLLQLLVWYLFFTDILPPISEPMTI
                     GGVIYLSKAGFSFPVPVWGLGQALAAAGAAICLPIAWWYRRWTIRQFELTARVRSVFW
                     APVAIVFAGSIIGWIAGGAPTAWNLPAPGAFSMEGGASATPEFMGVLLGLVLYTSAFI
                     AEVVRAGIASVAHGQVEAAGSLGLARSQTMRLVILPQALRVIIPPLTSQYLNLTKNSS
                     LAVAIGYPDVVSIATTVLNNTGRAVECITIIMLVYLVTSLSTAGFMNWYNNRTAIRER
                     "
     gene            10842..11951
                     /locus_tag="VFV55_08710"
     CDS             10842..11951
                     /locus_tag="VFV55_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX4944422.1"
                     /translation="MSVATVFTPIAPRPAPIRTTGLVPWLRANLFADWKSSLTTTVLA
                     LLALAWLPGVANWGIFQAVFHANLEGCQAARGSGACWGVIAEKYRLIILGRYPYGEQW
                     RPALATLLLMGLLGVSCTRRFWRPWLAPLWIAVLAGYFLLMRGGLLGLSYVPTDLWGG
                     LPLTIMLATLGIALAFPIAVLVALGRRSKLPAIRSMCIVYVELVRGVPLISVLFMASF
                     LFPLLMPMGKSPDVLLRVLAGITLFAAAYLAETVRGGLQAVPKGQVEAAATLGLTYWQ
                     TQRKIVLPQALAIVVPAVMNNFIAIFKDTSLVTIVSLYELTGSLGLALNADADWRPYK
                     LEGYLFIAAIYFAFCFAMSRYSLWIEKQVGASRNR"
     gene            11961..12704
                     /locus_tag="VFV55_08715"
     CDS             11961..12704
                     /locus_tag="VFV55_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX4944423.1"
                     /translation="MSEAIILFEKVNKWYGDDFHVLRDINLSVAGGERIVICGPSGSG
                     KSTLIRCINRLEEHQEGRIVIAGTELSDDVKAIERVRREVGMVFQQFNLFPHLTVLEN
                     LTLAPMWVGKTPKRKAEEIAMRQLERVRIAEQARKYPLQLSGGQQQRVAIARALCLTP
                     KIMLFDEPTSALDPEMITEVLEVMIELAGQGMTMLCVTHEMGFAKAVANRVIFMDQGQ
                     IVEESPPEEFFNRPKSERTKDFLSKILGH"
BASE COUNT         2036 a         4234 c         4353 g         2170 t
ORIGIN      
        1 tgcgcgatca gcgcatggcg cttcgggtcg tagagccaca ggccgtcctc catcgccgcg
       61 tacacgtcga tcaccatgat gtggcgccag cagggcgcgg tgcgatcgcc acccgggcgg
      121 ttgatgccgt cggcggccca gagaagatcg gagaggacct ggtcgggaag cttgcgcggg
      181 gagaactcgc gggtggagca gcgttgcgac aacgcctgca tcagcggcat gccgccttcc
      241 gtggccggcg caggcagcag cctcgctgta cccggtacag atgtcggtgc ggcggacgtt
      301 gcgagaggga ctagacccgg catgatgatc gccgcgccgg ccgcaccggt gttgctcagg
      361 aattcgcggc gggaataccg gcgggcggcg tgttcaccgc tccggtggcg aggggctgca
      421 gccatggcga cctcctcgga gcccgaaggc gccacggggc gcggcgctcg ggcggccagt
      481 agtttcctca tgctaggccg acccgccgtg cggtgcttga ttcgggtcaa cggattgtgt
      541 ctcgactccc gcccatccac tggggagaac gcagaattgc ctcaggcgac gtcgatctcc
      601 aggctccggt atcccaccct gcagctcccc accttgagcg tgagtttccc gcgcccctgc
      661 gccacgagag tgacgaactt ctcgttcacg ttgccgcgcg tccacgggaa gaagatgttg
      721 cttccggcgt acaggccgct gccccagccg tcgaggtggc cgatctcgac gatcgactcc
      781 gcgggcgcca cgagtttcac gcccgcgccc tcggtgggga gccgcagggg ttcggagatc
      841 gggagcttct tcgcggacgt tagcccgtag gttccgaggt agccgctgtt cgcgacgcgc
      901 acttcgatgc gcgtgtggtc cgcgccggcc ttctcctgct tcacgacttc cagggatacc
      961 tgcggcacga gggcggccac gcgaagaaac gcggcgctct gcgcggcgca tgtctcggcg
     1021 agcttctcgt aggggggatt ggagatgccc acgcgagcgt cgaagccgcc cacctcgacc
     1081 tcgccaagct gggggtgcct cgccttgcgc cagcggctga aaatgcggcc ttgattctgc
     1141 tcgcggtcga accgggcgag cgccaggtag tccttgcggt ccaggcgcgt gtaatggtcc
     1201 acgaagggct tcttgcgctc gatgccgagc tgccggaaga ggtcccacag ttccacgaca
     1261 tacgcgatgc acccgcgctg gtggtaggcg tactcggtga tgtcgccaaa gagcggtttc
     1321 tcgggctcgt agaggaactc gtggtagccg ctcaccgtcg cgtagccggt gtgctccgtc
     1381 atccatgcct cgacctggcg gaagagcgcg aggtccgact ggtccatctt gtggtcgggc
     1441 tggtcgccga gcgggcggat gagcacgccg ccgaacgtgt gcaggttgag ccacgcgaag
     1501 atgtgcgggt gcttcgtcgc gaactccatg atcgcccgcg tctccggggc gctccccggg
     1561 aaatgtccgg ccccctcctg cttcggctcc gcggcccact ggtaggggaa gttgcggttg
     1621 aagtcgtagg cgttgtcgga gaggaaatac gggtcgggga tgcgccggcc gtcgaagttg
     1681 acgatgcgcc cctccggata aagccggtag caggggcccg cgtcctcggg ccctcgcggc
     1741 accatgaccg ggggattcaa aggattgccg tcctcgccgc gaagctccac cagctccccg
     1801 tcggggcact cctgccgcat gaaacccatc acgccgtctc cgtcgatgtc ggccgcctcc
     1861 cagtacgcat ggtccctggg gacgcggtcg ttcacggggc tcgaccggac gtagcggccg
     1921 gtcttgagca ccgcctcggc gccgtcgggc gaaatgcgcg gcacgacgta gaagagcgcc
     1981 tgccggatgg cgtccgccat gtgcttcggc aacggcttgc cgccggcttc gtccttgccc
     2041 tggtggatgg cgacgatgtc ctcggccagg gcaagcgcca cgctcgagcc gcagagctcc
     2101 gatgcgtgca tgttgccgtc gatccatacg gcagggcgcg ccgcatccgg atcgcggccg
     2161 atggtgacga ggggaatgtc gcggccttcg gcgctcttgc ctatcgacgt cacccggacg
     2221 atatccgggt gcgccccgga ccaggcggcc agttgggcga agagctcggc gtagtcgagg
     2281 tacttggtgc ggaacatcct tcgctccttc cggagtcctt cgcggtcgac tcgttacaag
     2341 tgccgcgcct gcggcaaggc ggccatcctg cgatccagcg taagcgtgac gaggcccgcg
     2401 cgccggtaat cgtcggcaat gaggcgatcg agcaaaccgc agcctgcgtg cgtctccagc
     2461 gaagcgaaca cgcctgcccc gctgaaggga gacaccagtc cgctcctcaa gacgctggcc
     2521 aacgccgccc gcgcctccgc cttgggcacg ccgtagtggt gctgaagcgc gatgtaggtt
     2581 tccccgatca cctggttcga ggcgaacact tcggcgctgt cgcccttgac cagggcggag
     2641 agcttccgga cgcacatatc gaattcgcgc tccggatccc cggtggcgag ccggaccagg
     2701 atggaagtgt caattccgaa gcgaccgggc atggggcttt tcgcggaagg tctcgagatc
     2761 gaaggttccc tggccccggc gaagcttgcc gcgcagggga gcgagtcgcg tccggtcgat
     2821 ggcgcaggcg cgaagcacgt agccctcggg gctttccgag agttccagcc tgtccccggc
     2881 cttcgccccg agggcttcaa gcacgcgcgc cgggaaggtg acctggcgct tcgaggtgat
     2941 cttgacgaac atggaaggga actccggata ccttaccgat attgtatacc ggtaaggaag
     3001 ccggctcgag ggagccggct ccgttccatc aacgcgagat accggcagcc cgttgacccc
     3061 cggctgccgg gaatccgcgt tcgcggactt cagtcctcca ccaccagcac gagggcgagc
     3121 accgccgcga tggccgccgg gatgccgtag cgttcccatt tcgacatcga gtcgccggcg
     3181 atcagttcca ccggcaccgt cgccagctcg aggacctcgt tggtgaccga gctctcgacc
     3241 agccgcgtga gggagttctt gcgcgccgtc gccatcacga tctggtcgca gcgcaggcgc
     3301 cgtgcctcgt cggcgatcac tttcgcccgg tcgcccacct cgatgtgcat ggaatacgga
     3361 atggaaaagt tgtcgagcat ctgcctgacc ggctggagcg ccttttcccc ctgctcgcgg
     3421 tggtagtcct ggcggctcat gcggctcgtg aaatgcgcga tatccgcgga gaacggcgcc
     3481 tggacgttga ggaggtgcac ctccatcgcc gtgttgttca tgaactgctt gatgacgtgc
     3541 tggacggcaa acttgcagtt tccggagcca tcgaccggga cgagtacctt gagcatgatg
     3601 ttccctccag tgactggttg cgggacgggc aggagcgcct tgacccggac cggcgcatgc
     3661 cggcggcgga agtagatctt gagcagttcc atggccacgg gcgaagcgaa gaagaacggc
     3721 agcatcagca gccagtcggc cccgctcagc ggcacggtat cgaagaacgg ctgcaggaac
     3781 ggcacgtaca ccaccgcgag aaccagcacg aaggacaccc cgacggccgc gaccatccag
     3841 cgattggaga acacgccgat ggagaacacg ctgtggtact cggaacgggc ggcgaacgcg
     3901 cgaatgagct cggaggtgca caacgtgacg aaggcgatcg tctgcgccgc ctcgagatga
     3961 tccgggtagc gctgcatggc gaggtagaac accgcgagga tcgcgatcat gtccacgacg
     4021 cccaccacgc cgatgcctac cgccatgtcg cggttgatga cgggctcctt cggcgagcgc
     4081 gccggctgcg tcatgatgtc gttgtcgccc ttctcgagcc cgagcgccag ggcgggcgcg
     4141 ccgtcgctca cgaggttgag ccacaggagc tgcacgggcc gcaggggcat cggcatgccg
     4201 aagagcatcg cgccgaacac gatcaggatc tcgcccacgt tgcaggcgag caggaagtac
     4261 acgaacttgc ggatgttcga gtagatgatc cgcccctgct cgatggcggc gacgatgctc
     4321 gcgaagttgt cgtcggtgag caccatgtcg gccgtctgct tggcgacgtc cgttccggtg
     4381 atgcccatgg ccacgccgat gttggcgcgc ttgagcgccg gcgcgtcgtt cacgccgtcg
     4441 ccggtcatcg ccaccacgtg gtcgcgcgcc ttgaaggcct cgacgatgcg cgtcttgtgc
     4501 tgcggcgaga cgcggcagca gacttccagc cggtcggcgc gcgccgcaag ctcctcgtcg
     4561 gagagcttgt cgatctcggc cccggtgagc accagcccgt cgggcgtgcg caggccgata
     4621 tcgcgggcga tggcctcggc ggtgtccttg tagtcgccgg tcaccatcac ggtcttgagg
     4681 ccggcgcccc gggccacctt gagcgccgcg atgacttccg gccgcggcgg gtcgatcatg
     4741 cccaggaggc cgacgaagac gaggttcttc tccaccgtct ccgggctcac cgtcgccgga
     4801 atctcgtcca gtgcgcggta ggccacgccc agcacccgga gcgcgtcgct cgccatgtcg
     4861 cgattctggt cgaggatcga gctgcgcatc gcgtgcgtga gctccaccgc ccggccaccg
     4921 tccagcatgg aaccgcacaa ctcgaggacg atgtcgggcg cgcccttgac gaacgcgacc
     4981 agcggcggaa agtcgaagcc cggctgggcg gtgcccgagg cgtcgttgcg gtgaatcgtc
     5041 gtcatgcgct tgcgctcgga gtcgaacggg atctcctgga cgcgcggcat cgacttcgcg
     5101 aactcgtcct gcaggtagcc gctcttggcc gcggcgacga ccatggcgcc ctcggtcgga
     5161 tcgccgatga tgttccacga gcgcgcgccc gactcgtcgg ggcgctcttc cagcatcgcg
     5221 tcgttgcagg cgatggcgcc ctgcagcaac gccgtcgcat ccgggttcgt acgcgggtcg
     5281 aagcgctcct cgccgaggaa gaactcgccg gtcgggttgt acccctcgcc ggtgatgcgg
     5341 aagcgccggc cgccggccca ggcctggacg accgtcatct ggttctgcgt gagggttccc
     5401 gtcttgtcgg agcagatcac cgtggcgcac ccgagcgtct ccaccgcggg cagcttccgg
     5461 atgagcgcgt ggtgcttgat catccgctgc atgcccagcg ccaggcagat ggtcacgatg
     5521 gcgggaaggc cctccggcac cgcggcgatg gcaaggctca ccgccgtcat gaagaggttg
     5581 atgatgtcct tccgctcggc ctccaggtag gcgagcaggc cgatgttggc gacgtccgcg
     5641 atatgggtgt cgcggaacag cccgtagacg aagacgatcg cgcagatggc aaggcaagcc
     5701 gtgccgagga ccttgcccaa atgctcgagc ttcttctgca ggggcgtggc ctcgtcctcg
     5761 aaggactgga tcatctgcgc gatgaggccg atctgcgtgt tcatccccgt gcacgtgacg
     5821 aggcccctgc cgcggccgta ggtgatgagc gtgcccatga acgcggtgtt cttgcggtcg
     5881 cccagcggga tttccttgtc cagcaccacc gcggggttct tgtcgaccgg caccgactcg
     5941 ccggtgaggg aggcctcctc gaccttgagg ttgtggctct cgacgaggcg cacgtcggcc
     6001 gggacgtagt tgcccgcctc cagcagcacg atgtcgccgc cgacgatctc gcgtcccggt
     6061 atcgtgacct ggtggccatc gcgtatcacc tgggcacttg gggcggccat cttcttgagc
     6121 gccgccagcg cctgttccgc cttcgattcc tggatcacgc ccaccaccgc gttgaggacg
     6181 acgatgacca tgatggcgat ggaatcgacc cattcgccca gggccagcga cacgacggcc
     6241 gcgatcacca ggatgatgac gaggtagttg ttgaactggt cccacagcag cgcgagaaag
     6301 ccgggccgcg ggcgttcggt gagttcgttg gcgccgtgcc tggcgagccg gtccagcgca
     6361 tcctgctggg tgatcccccg ttcgaggtgc gtctcgaaat gcgcgacgag atgctcgatg
     6421 ggcttgctgt gcgaatccct gtcttgcatg gcgggttcct ccttgatgct tgtgagccga
     6481 gtattgcgcc gtgccgagag gaagaaaatt tgaactttgc aaggccagcc attacgaaaa
     6541 cgcaggtcgt ccggcaattc gtaatggcag gaatcacgat tcctgatgga tggcgcccgg
     6601 aattgcgccg cgacttgccc ggcgggcttg aaacggcgcc ccgggagtgc tagaagtgga
     6661 ccacgccccg gcgaatggcc ggggctccct ccaaagggga gtagcttcgg cagccggcat
     6721 tcggctgctg cgacgcggcc gtcaatacgg gttcgccccg gtcgcatcgg cattctcgat
     6781 atgcaagcga gacctttgcc cagcgggtga aggtgcgtcc tgacggcggc cttcatcccc
     6841 gaggtgaagg ccgctgtgca tttgcaggca cggcggcatg gccatagaga atagggagcg
     6901 cgccatgctg caaccgaaca tccaccgcta cctccggcac ggaatgcttc cgcagcttcg
     6961 cgtcttcgag gcgagcgcgc gcctgggcag cttcacccgc gccgcgcagg aactgcacat
     7021 ggcccagccg acggcctcgg tgcagctcaa gaagctctcc gagacggtcg gggttccgct
     7081 cttcgagcag gtgggcaagc gcatttacct caccgacgcg ggccggcgcc tctacctggg
     7141 ctgccaggag gtgttccgcg ccttttccgc catcgaggaa tcgctcgccg gcatgcgcac
     7201 gctggaagcc ggtcgcctgc ggctgggcgt gagcacgacg ggcatgtgct tcgccccgcg
     7261 gctgctcggc gcgttcgtcg ggctccaccc cggcgtcgag atttcgatgc aggcgcacaa
     7321 ccgccagacc ctcgtcgagc gcctcgccgg caacgaggat gacctctacc tcttcgcgga
     7381 cgcgccggaa ctggaggacg tggtggcgca ggagctgctg cccaatcccc tggtggtgct
     7441 ggcgcgggac gaccatccgc tcgcggcgga gaagaacatc gccttcgaac gccttgcccg
     7501 ggagccgttc ctcgtccgcg aggaaggatc gggcgcgcgg cggatcatga tgcggctgtt
     7561 ccaccagcac gggctcgtcc cgaaggtccg gatggagctg ggcaccaacg aggcgatcaa
     7621 ggaagcggtc ctcgcgggcc tgggcatcgc catcatgtcg cgctacacgt tcgggctgga
     7681 tccggagtcc tcgcggtatg tgtgcctgga cgtcgcggga tttccgatcg agaaccactg
     7741 gtacttcgtg tatcccgcgg gcaagcagct cacggcagcg gcgcgggcct tcctcgactt
     7801 cgcgcggacg caggccaagg ggctggtgcg cgagagcctg gccaggcact aggcggcgag
     7861 tcgcggtggt ggcccgttgc gcatcgcttg cgttttcccg gcaaaggtcc accatgggat
     7921 cgggccattc atgccaggag actgcaatgc gtgcgaaacc cttgttgatt gcgaccgtgg
     7981 ccgctgccgc gatgaccttt tccacaggcg cttccgcggg cgatcccctg gccggcgcgc
     8041 tgatcggcgg aggcatcggc gccgccgtgg ccggcccgcc cggtgcggcg gtgggcgcga
     8101 tcatcggcac catcgcgggg agcacccacc cgtactacta cggggaacgc ggatattacg
     8161 atcgcgggta ttacgatcgc gacacctacg ctcccgaacc cgcctattac gcacccccgc
     8221 ctgcgtacta cgccccggca ccggtctatt acgccccggc gcgggtttac tacgcgccgc
     8281 ctcccgtcta ctacggtccg tcggttgcct atcgcgcggc gcccgcgtac gtgtacggta
     8341 cgcgccatgc gacgtatgac cgccgcggcg accggcaccg cgacgggtac cgcgacgggc
     8401 acgatggcgg gcgataccgg gatgggtacg accgacgttt ccgctgaaat ccgcgtctga
     8461 cccgcagcga gaaggccccg cgcatgcggg gctttttcgt gggctgtcgc ccccgcaatt
     8521 ccggatggac cgcaacggca ccggctcgat tggtaagcga gccccaccgt agtacatttg
     8581 ccaacgcaca ctttcccgag gacaggacga ccatgaaaac gacgacccgt aaaccgatcg
     8641 cgctggctgg cctggtggcc gcggcgctgc ttgccgcggt gccggcccat gccggaaaga
     8701 cgctggacgc catcaaggcc cgcggccaac tggtgtgcgg cgtgcacacg gggctggccg
     8761 gcttctccca ggccgacacg cagggcaact ggagcggcct cgacgtcgac gtctgccgcg
     8821 ccatcgccgc tgccgtgctc ggcgacggga gcaaggtcaa gttcgtgccg ctcaccgcgc
     8881 agcagcgttt caccgcgctg cagtcgggtg agatcgacat cctgtcgcgc aacaccacct
     8941 ggacgctttc gcgcgacgcc tccctgggcc tgcatttcat cggcaccacg tactacgacg
     9001 ggcagggttt catggtcccg gtgaagacga agatcaagag tgccaagcag atgaagggcg
     9061 ccacggtgtg cgtgcaatcc ggcaccacga cggaaaagaa catgaccgac ttctcgcgcg
     9121 cgaacaagct ggacctgaag cccgtcgtgt tcgagaagat cgaggctgcc accggcgcct
     9181 acttctccgg gcgctgcgcg gcgttcacga ccgacgcctc gggcctcgcc tcggtgcgca
     9241 acaaggaagc gaagaaccct gccgagcacg tcatcctgcc ggagctcatc tccaaggagc
     9301 cgctcgggcc cgccgtgcgg cgcggcgacg acgagtggtt cgcgatcgcc aagtgggtgg
     9361 tgttcggcct gctcgagagc gaggagtacg gcatcaccca ggccaacgtc gagaagatga
     9421 cgaacgagag ccaggacccg gtggtgatgc gcatcacggg caagtcggag gacatgggca
     9481 agctcctggg cctggaacgg gactggatgg tcaaggccgt gaaggccgtc ggcaattacg
     9541 gcgagatctt cgaccgcaac gtcggcccca agacgcctct cggcctgccg cgcgggatca
     9601 acgcgcagtg gaacaagggc ggcctgatgt acgccccgcc gatccggtaa gcgcgcggca
     9661 ccgccgatga gcgggcaccc gccaccgcgg cgcagggact ggtcgtggcg cagccaggca
     9721 ttccgcgggc tcgtctacca ggtcctggcc gcggcggtca tcgggctggc gctctggttc
     9781 ctcgcgcaca acaccgtcga gaacatgcgg gtgcggggaa tccagagcgg tttcgggttc
     9841 ctcgtgcagc ccgccgggtt cgacatcggc gaaagcctca tcccgtacga cgcgctcgac
     9901 cgcaactgga gggcattcct cgtcggcgtg ctcaacacgc tgcgcgtggc catcgtcggc
     9961 gtggtgctcg cgaccgtgct gggcgcgctg ctcggcgtcg ggcgcttttc gcgcaacgcg
    10021 atcgtgcgcg gcctctgcta cgcgtacgtg gaactcttcc gcaacatccc cgtgcttctg
    10081 caactgctgg tgtggtacct gttcttcacc gacatcctgc caccgatctc ggagcccatg
    10141 acgatcggcg gggtgatcta cctgagcaag gccgggttct ccttcccggt gccggtgtgg
    10201 gggctggggc aggcgctcgc cgcggcgggc gcggcgatct gcctgccgat cgcatggtgg
    10261 taccgccgct ggaccatccg ccagttcgag ctgacggccc gggtgcgcag cgtcttctgg
    10321 gcgcccgtcg cgatcgtctt cgccggatcg atcatcgggt ggatcgcggg cggcgcgccg
    10381 acggcctgga accttcccgc gccgggcgcg ttctcgatgg aagggggcgc gtcggcgaca
    10441 ccggaattca tgggcgtgct gctcgggctg gttctctaca cgtcggcctt catcgccgag
    10501 gtggtgcggg ccggcatcgc gtcggtggcg catggacagg tggaggcggc cggcagcctc
    10561 gggctcgccc gctcgcagac gatgcgcctg gtgatcctgc cgcaggcgct gcgcgtgatc
    10621 atcccgccgc tcaccagcca gtacctgaac ctcaccaaga actcgtcgct ggcggtggcc
    10681 atcggctatc cggacgtggt gtcgatcgcc acgaccgtgc tcaacaacac cgggcgggcg
    10741 gtcgagtgca tcaccatcat catgctcgtg tacctggtga cgtcgctctc gaccgccgga
    10801 ttcatgaact ggtacaacaa ccgcacggcg atcagggagc ggtgagcgtg gccaccgtct
    10861 tcacgcccat cgccccgcgc ccggccccga tccgcacgac ggggctggtg ccgtggctgc
    10921 gcgccaacct gttcgccgac tggaagagct cgctcacgac gaccgttctg gcgctgcttg
    10981 ccctcgcctg gttgcccggc gtggccaact ggggaatctt ccaggcggtc ttccacgcca
    11041 acctggaggg ctgccaggcc gcgcgcggca gcggcgcctg ctggggcgtg atcgcggaga
    11101 agtaccggct catcatcctc ggccgctatc cttacggcga gcagtggcgc ccggcgctcg
    11161 ccacgctgct gctgatgggc ctgctcggcg tcagttgcac gcgccggttc tggcggccgt
    11221 ggctggcgcc gctctggatt gccgtgctgg cgggctattt cctcctgatg cgcggcggcc
    11281 tcctggggct ctcgtacgtg cccaccgacc tgtggggcgg actgccgctc acgatcatgc
    11341 ttgccacgct cggcatcgcg ctcgcgttcc cgatcgccgt gctggtggcg ctgggacggc
    11401 gatcgaagtt gccggcgatc cgctcgatgt gcatcgtgta cgtcgagctg gtgcgcggcg
    11461 tgccgctgat ctcggtgctg ttcatggcct cgttcctgtt cccgctgctg atgcccatgg
    11521 gcaagtcgcc cgacgtgctg ctgcgggtgc tggcggggat cacgctcttc gccgccgcct
    11581 acctcgccga gacggtgcgc ggcgggctgc aggcggtgcc caaggggcag gtggaggcgg
    11641 ccgccacgct cggcctcacc tactggcaga cgcagcgcaa gatcgtgctg ccgcaggcgc
    11701 tcgcgatcgt ggtgccggcg gtcatgaaca acttcatcgc catcttcaag gacacctctc
    11761 tcgtcaccat cgtgagcctg tacgagctca ccggctcgct cgggctggcg ctcaacgccg
    11821 atgccgactg gcgcccctac aagctggaag gctacctgtt catcgcggcg atctatttcg
    11881 ccttctgctt cgcgatgtcg cgctacagcc tgtggatcga gaagcaggtg ggcgcgagca
    11941 ggaaccgcta ggacccggcc atgagcgaag cgatcatcct tttcgagaag gtcaacaagt
    12001 ggtacggcga cgacttccac gtgctgcgcg acatcaacct ttcggtcgcg ggcggcgagc
    12061 gcatcgtgat ctgcggtccc tcgggctcgg gaaagtcgac gctcatccgc tgcatcaacc
    12121 ggctggagga gcaccaggaa ggccgcatcg tcatcgccgg cacggagctc tccgacgacg
    12181 tgaaggcgat cgagcgcgtg cgccgggaag tgggcatggt gttccagcag ttcaacctgt
    12241 tcccgcacct gacggtgctg gagaacctga ccctcgcgcc gatgtgggtg ggcaagaccc
    12301 cgaagaggaa ggcggaggag atcgccatgc gccagctcga gcgcgtgcgc atcgcggaac
    12361 aggcgcgcaa gtacccgctg cagctatccg gcggtcagca gcagcgcgtc gcgatcgcgc
    12421 gggcgctgtg cctcacccca aagatcatgc tgttcgacga gccgacctcc gcgctggacc
    12481 cggagatgat caccgaagtg ctcgaggtga tgatcgagct ggccgggcag ggcatgacga
    12541 tgctgtgcgt gacgcacgaa atgggattcg cgaaggcggt ggccaaccgg gtgatcttca
    12601 tggaccaggg gcagatcgtc gaggaaagcc cgccggagga attcttcaac cgcccgaaga
    12661 gcgaacggac gaaggacttc ctgtcgaaga tcctggggca ctgaccgttt ccgggcctag
    12721 tgatgtaccc ggtacatctc cagccgcgcg agatccggct ccacgcccag ccccggcgca
    12781 tccgatagcg tta
//
LOCUS       DASUEV010000270        19304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1573704, whole genome shotgun sequence.
ACCESSION   DASUEV010000270 DASUEV010000000
VERSION     DASUEV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573704"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1432)
                     /locus_tag="VFV55_08720"
     CDS             complement(14..1432)
                     /locus_tag="VFV55_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX4944424.1"
                     /translation="MPRLKDHDAIRRIAKKSLFEGLDAMCEGAIIVDRDARIVWISDK
                     YATHLKLASPAEAAGRVVEDVIPNSLMREVVRTGEPMMLDIMQFGDKSFVVMRVPVKD
                     ERGRVIGAAGFMLFDTLASLQPLVRKFQQLENELASTRRSLAEERRVKYTFSNFIGTS
                     PGAMEVKRQARRAAQADTTVLLLGETGTGKEVLAQAIHAASPRAHGPFIGVNVAAVPE
                     TLLEAEFFGTSPSAYTGADRRGRDGKFMLAHGGTLFLDEVGDMPMSMQAKLLRVLQEK
                     EVEPLGSNRVAKVDVRVIAASSVDLRQMVGMGRFRSDLYYRLSVLPIELPPLRDRISD
                     LEALCEHVLEDIARRSGRPQRELAPTALAVLSSYAWPGNIRELRNVLEQVTMRSDAAR
                     LSAEEFSLVLPRVASAVHESGRPTLRLADIVADAERNAIRSALAAADGKKILAAELLG
                     ISRATLYEKLSSLAPRGHEHRH"
     gene            complement(1486..2187)
                     /locus_tag="VFV55_08725"
     CDS             complement(1486..2187)
                     /locus_tag="VFV55_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX4944425.1"
                     /translation="MIDLYYWTTPNGHKITLYLEEAGLPYRLHGINISKGDQFAPAFL
                     KISPNNKIPAIVDHEPADGGKPLSVFESGAILLYLAGKTGKFIPKDLRGQVEALEWLM
                     WQMGGLGPMLGQNHHFAVYAPEKIPYAIDRYVKETNRLYGVLDKRLAGRDFILGRHYS
                     IADMAAYPWIIPERQGQDLADFPNVARWHAAIRARPGTTRAYAKAKEVNPDPRGPVTD
                     KERKILFGQDKSVVK"
     gene            complement(2184..2939)
                     /locus_tag="VFV55_08730"
     CDS             complement(2184..2939)
                     /locus_tag="VFV55_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX4944426.1"
                     /translation="MIAFVTGATAGFGAAIARRFAKEGHRVVAAGRRAERLAALREEL
                     GRAVHTVVLDVRDRAAVLEAFKALPPAFAEVDVLVNNAGLALGLEPAQKANLDDWDRM
                     VDTNVKGLLYCTRAALDGMVARNRGHIINIGSIAAEFPYAGGHVYSATKAFVHQFGLS
                     VRADLLGTAVRLTDVQPGMVAGTEFSEVRFKGDQARAQQVYQGTEPLTAEDIADCVYW
                     AATRPAHVNVNTIQVMPVCQAPGRVAIHRKETP"
     gene            complement(2975..4051)
                     /locus_tag="VFV55_08735"
     CDS             complement(2975..4051)
                     /locus_tag="VFV55_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX4944427.1"
                     /translation="MSLLFSPVALRSVTIPNRVFVSPMCQYSCEDGMPNDWHLVHLGS
                     RAVGGTGLVFTEAASVSPDGRITPWDAGIWSAQHAAAWKPIAAFIKAQGVVPGIQLAH
                     AGRKASCDKPWNGGGPLARDAGGWKTHGPSDVAFGNYPAPRAMSAPDLEACVADFRLA
                     ADRALQAGFEVAEVHAAHGYLLHEFLSPLSNLRKDDFGGSLSNRMRFPLAVVRAVRDA
                     WPQDKPVLVRISASDWKEGGWDLEQSIAFARELKELGVDMIDVSSGGNAADQKVVLGP
                     GYQAPFAAAIRRDAGIATAAVGLITEPVQAEHILATGQADAVCLARALLRDPYWARHA
                     ARALDEAMPWPNQYKRADVGPLGR"
     gene            4140..5255
                     /locus_tag="VFV55_08740"
     CDS             4140..5255
                     /locus_tag="VFV55_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4944428.1"
                     /translation="MDAITIRAGPRALAAIRANGLRAADVAIVPGAAGGPKALGLHGL
                     DVAIFGEWFPKAPRVRHLIGASIGAWRFAAACRPDAAEGLRELARLYIEQRYPPRPGA
                     ALVTRKVREMLAELFDGHDAEILASQGYRLHILAVRGRGLLSRDAGMRRTLGFGAAVF
                     ANLAGRRHLRHFLERTVFHDARDWPSFLDDAKVAGSLLPASGAAPIRFDAFRTHAVAL
                     DRDNLREALVASAAIPMVIEAVPDIPRAPSGVYWDGGLIDYHLHLPYSRSDGLVLYPH
                     FTDRIVPGWLDKALPWRRARDEWLDNVVLVSPSREYLAALPSGRLPDRSDFRRFESDF
                     EGRVARWRRAIAESARLGDEFLAFAERPDPARVLPLE"
     gene            complement(5370..6338)
                     /locus_tag="VFV55_08745"
     CDS             complement(5370..6338)
                     /locus_tag="VFV55_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944429.1"
                     /translation="MTRLLRGVLAVLIVVSLPALADWQPTKPVRIVVPFAPGGQPDVI
                     ARALSEPLSKALGQPVVVENRPGAGGNIAADAVAKSAPDGHTLLMGTNGPLAVSPALA
                     KNLAYDVGRDFAFVTLVGTSPSLIAVHPSLGAATLAEVVAKAKAQPGKLNFASVGKGS
                     VSQLSMEQLNAAAGIDTMHIPYNGGAPAVAALLAGDVQLLSLNPTALIAHVQAGRIRV
                     VAQTGARRSPLIPDLPTVAESGFPGFEAPVWMAIVVPARTPPEAVKRLHAELAKIIRA
                     PELKARLWDKQWIDPLGSTPEEAARVVDAETRKWARIGRALNLTLE"
     gene            complement(6335..6550)
                     /locus_tag="VFV55_08750"
     CDS             complement(6335..6550)
                     /locus_tag="VFV55_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944430.1"
                     /translation="MNPDPDEIDWSLTTWEGSRRAQIRLWMSFTVRERLEMIEGMAEL
                     AKHLRECGEREIGRGAVASEPRPRDLK"
     gene            complement(6534..6917)
                     /locus_tag="VFV55_08755"
     CDS             complement(6534..6917)
                     /locus_tag="VFV55_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944431.1"
                     /translation="MNVATLEAIARALDRPGFRPTVPITAEQFADGAQRERFRTEKGM
                     LVLNLYSNHYRDTTVDIFVAEPFDFDAEYARAGLQEIGPGLSVRFVTIPTLIDMKIKA
                     GRPRDLDDVEHLRMILDEKSRESGS"
     gene            complement(6958..10662)
                     /gene="metH"
                     /locus_tag="VFV55_08760"
     CDS             complement(6958..10662)
                     /gene="metH"
                     /locus_tag="VFV55_08760"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX4944432.1"
                     /translation="MDQALATRPARIQALHDALATRILVLDGAMGTMVQRYRLTEAEY
                     RGERFRSFGHDLRGNNDLLTLTRPQVIREIHAAYMEAGADILETNTFNSTTISQADYH
                     LEAIVAELNREGARLARAVADEYTAKNPGKPRFVAGVLGPMSRTLSLSPDVNDPGFRA
                     VDFDQVKDAYLEAARALVEGGADIILVETIFDTLNAKAALFAIDECFEQTGERLPIMI
                     SGTITDASGRTLSGQTPEAFWNSVRHARPLSIGFNCALGARQLRPHIEELARIADTLV
                     SAHPNAGLPNAMAEYDELPEETAAFIREWAEAGWLNITGGCCGTTPEHIRAIADIVSP
                     CRPRTIPKIEPKLRLAGLEPQNIGDDSLFVNVGERTNVTGSKAFARLILAGDYAEALA
                     VARQQVENGAQVIDVNMDEAMLDSRAAMTKFLNLVAAEPDIARVPLMIDSSKWSVIEA
                     GLKCVQGKSIVNSISLKEGEAEFVKHAKLARRYGAAVVVMAFDEKGQADTLERKKSIC
                     ERCYRILTEQVGFPAEDIIFDANIFAIATGIEEHNRYALDYLEALAWIHRHLPHVKTS
                     GGVSNLSFSFRGNEPVREAMHTAFLYHAAKAGMTMGIVNAGQLGVYADIPADLLERVE
                     DVIFDRRPDATERLVTFAESFKGQKKDQVEDLAWRDAPVEQRLSHALVKGITQWIVED
                     TEEARRKFPRPILVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPYI
                     EEEKRLLGSADAKAKGKVVMATVKGDVHDIGKNIVGVVLQCNNFDVVDLGVMVPAAKI
                     LETATREKADMIGLSGLITPSLEEMAHLAGEMERLGFRIPLLIGGATTSRTHTAVKIE
                     PHYSGPTVWVPDASRAVGVAASLVSEDQRDRYVSQVRSDYLKVRDAHAKKAGQKSIPV
                     EAARANAVKLDWAGYTPPAPRHRGLLSLKNYPLDKLVPFIDWGPFFQSWDLAGKFPDI
                     LTDVVVGEAASNVYAEGRAMLERIVKGRWLTANAVLGLWPANSVGDDIEIYSDETRRH
                     VLMTWHNLRQQNEKPAGNPNQCLADFVAPKDSGIADYLGAFAVTAGVGIEARVKEFEA
                     RHDDFSAILLKALADRLAEAFAEHLHHRVRTEFWGYSADEKLAAEDLIAEKYAGIRPA
                     PGYPACPDHTEKGDLFRLLEADTNAGIALTESFAMYPTAAVSGFYFSHPASRYFAVGK
                     IERDQVADYAHRKGMDIAACERWLAPVLNY"
     regulatory      complement(10705..10783)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(10872..11093)
                     /locus_tag="VFV55_08765"
     CDS             complement(10872..11093)
                     /locus_tag="VFV55_08765"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX4944433.1"
                     /translation="MARIVKRTHTEPLKLVLGGEEKFLCRCGLSRNQPYCDGSHKLTK
                     GEEPNRLYWYDDSGQRHEYAGELPGIRTF"
     gene            complement(11109..12050)
                     /locus_tag="VFV55_08770"
     CDS             complement(11109..12050)
                     /locus_tag="VFV55_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944434.1"
                     /translation="MHAVRSLLASLVSLAFLFAPTPAGAQYPAMSYQGLWWRSPAGSE
                     SGWGVNIVHQGDILFATWFTYDTDGTGLWLVMSEGRRDISIEMDPYYGGYGMGGYGMM
                     DPNTKSYTGNLYRTTGPAFDSAHFDPAMVTVTPVGTANFAFTSADRGTFTYTVNGAMQ
                     SKPIVRQVYALPMPNCMVGGSRGASPNYQDLWWGAPAGSESGWGINVVHQADTLFVTW
                     FTYDASGRGMWLVMSDGRKTGAATYSGALYHTTGPAFSAATWDGSKVALDQVGTATLS
                     FADPDNGTFTYTVNGVTQVKAIVRQVFSTPVTNCTSP"
     gene            complement(12305..12805)
                     /locus_tag="VFV55_08775"
     CDS             complement(12305..12805)
                     /locus_tag="VFV55_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944435.1"
                     /translation="MRVIDARAALMGSHRTGAGGESEEIPMDQEATKIYAKLAEFEKE
                     IANLREGYLVVGKRYSESIESLKVLTDHAREAAERAAASAILSATAATNAATAAREAA
                     NETVVAAAAAASAAAAQQAEEVSVQASAHATLAAKRAAEAAAEAVRMAHAASEAAQSV
                     KGKPPA"
     gene            12907..13596
                     /locus_tag="VFV55_08780"
     CDS             12907..13596
                     /locus_tag="VFV55_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEX4944436.1"
                     /translation="MKPAIALATVLLAAAGFAAQAMPSQQAATLKGEVLETLNVESYT
                     YLRLKTQDGETWAAVPTTAVKKGAQVTIGNTMVMENFESKALKRKFDRIVFGAIADPN
                     AKPAAPAGSHGSAPVASVPVAKVAKATGPDAKTVAEVVKGKASLKGKPVLVRAQVVKV
                     SSGILGKNWLHLQDGSGSAADSTNDIIVTTTDKAAVGDIVNAKGTVRTDVNVGSGYAY
                     AVLIEDAALRK"
     gene            13612..14121
                     /locus_tag="VFV55_08785"
     CDS             13612..14121
                     /locus_tag="VFV55_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4944437.1"
                     /translation="MKAILLLALWIGAWPAALAATPGEVPVGGTLREAPLYGLTLDYR
                     RLSEFRGKPLVINVWASWCGPCRDEMGSLDRLARRHNGRGLNFIGISTDDYADKAQAF
                     VADTGVSFDNYLDKNLLLETMLGANRLPLTVLVDARGKVLKKVYGARQWDSPENKALI
                     AGTLGVKLD"
     gene            14205..15356
                     /locus_tag="VFV55_08790"
     CDS             14205..15356
                     /locus_tag="VFV55_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4944438.1"
                     /translation="MRTWAFLPLSILLGACAFSPPAPESSRFLADQLFGAPSERVDAG
                     TVFAASESMKRYLRDEIGILPGSKGRQQVLIEKLRSKGQLKLEYDAAFTRNAAQAFEA
                     RTGNCLSLVIMTAAFAREMGIEVRYQNVFVDETWSRQGDLYFSIGHVNLTLGRRPPAL
                     GTRIDDGEQLTIDFMPPRDTRGMNWRVIGEKTILAMYMNNRAAESLTAGKTDDAYWFA
                     REAILQDPEFMTPYNTLGVVYQRSGHLGQAERIFAYALEREPRNTHAMSNMATVLAGL
                     GRVAEAQALERRLAQLEPEPPFSNFNRGVAAMREGDFRAARDLFAKEVDRDPGYHEFH
                     FWLALALANLGETERAKRHLALAMEYSTTRKDHDIYAAKLDRIRSSRLH"
     gene            complement(15339..16124)
                     /locus_tag="VFV55_08795"
     CDS             complement(15339..16124)
                     /locus_tag="VFV55_08795"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX4944439.1"
                     /translation="MGPIRIGLIVVAGLGAVAAAWYFWPAAQPPTIPPPVAQVKPAAA
                     PESAPAEHYPVPESAEAEPAKPLPALDASDKELQDALAGPLGADALARFFVPERLVRN
                     IVVTIDNLPRRTFAMRLSPLKPVGGLMATTGKDESLAIAPGNAARYAPWMLLLEKVDA
                     RKLVAVYVRFSPLFQKAYVELGYPKGHFNTRLVQVIDHLLTAPEPAAPVRLAVPHVLP
                     EYADPALQSESAGRKVLFRIGNANAAKVKVKLREIRRELVKAR"
     gene            complement(16201..18126)
                     /locus_tag="VFV55_08800"
     CDS             complement(16201..18126)
                     /locus_tag="VFV55_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4944440.1"
                     /translation="MPLLQLSQVCLAYGHVPLLDHADLVIEAGERVGLIGRNGTGKSS
                     LLKIIEGTGRADDGKVWLASGIKLAFVPQEPAFPPGLTVFEAVAEGLGEGTRLLVEYH
                     DATHALAAHPGGPSEEALMARLHELQEALEAAGGWTLQHRIEATLTRVALPEDAMVDD
                     LSGGMKKRVALARALVLAPDLLLLDEPTNHLDIAAIEWLEETLVSFTGAVLFVTHDRR
                     FLDRVAQRIVELDRGRLADYPGNFSAYEARKAEQLAVEAVVNRKFDKVLAQEEAWIRK
                     GVEARRTRNEGRVRRLEALRLERAARRERVGKVGIALSPGERSGRLVTELEDVTKRFG
                     EKRVVEKFSTRILRGDKIGFIGPNGCGKSTLLKLILGRLAPDAGSVRLGTKVAVAYFD
                     QFRAALDEEATLADTISPGSDFIEIDGGRKHVIGYLGDFLFPPERANAPVKTLSGGER
                     NRLLLARLFSRPANVLVLDEPTNDLDIETLELLESLLQDYAGTLFLVSHDRAFLDNVV
                     TQTIAFEGEGRWKEYAGGYGDWQRARTGASRDARKPPVEGGADESPRTDTRAPRRKTK
                     LGFNELRELEQLPGKLEALEREQKELADRLGDPAIYQDRSVDLKALNERHAAIEGELT
                     RLLARWEELEAKKAALS"
     gene            18238..>19304
                     /locus_tag="VFV55_08805"
     CDS             18238..>19304
                     /locus_tag="VFV55_08805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX4944441.1"
                     /translation="MPSISRRADQLGTENAFVVLAEVSKLQAAGKDIISFCIGQPDFP
                     TPVNIQDAAVKAIRAGKHGYTPSAGIAELREAIAAYVSRTRGISVSADDVVVGAGAKP
                     FIAYTILSVTDHGAGDEVIYPNPGFPIYESQIRAMGAVPVPIYLRESRGFNFDPSELE
                     AKITPRTKLLILNSPQNPTGGIVSGKDMQAIAAILARHPNVWVYADEIYSRLVYDGEF
                     ASLAAIPGMQERTIVCDGASKTWAMTGWRLGYAVNKALAPVFTRWVTNTESCASHISQ
                     WAAVEAINGPQHDAEKMRASFLARRDLIVGLLTQVPGVTCQSPGGAFYAWPNVTEACR
                     LVGAKDSEEFRVRLLNEAGVAV"
BASE COUNT         2941 a         6643 c         6536 g         3184 t
ORIGIN      
        1 tcaggccgat cgctcaatgg cgatgctcgt ggccgcgtgg cgcgagggag gacagcttct
       61 catacaaggt cgcgcgcgaa atgccgagca gctccgcggc gaggatcttc ttgccgtcgg
      121 ccgcggcgag cgccgagcga atggcgttgc gttccgcgtc ggcgacgatg tcggcaagcc
      181 tgagcgtcgg cctgccgctt tcgtgcaccg cgctcgccac ccgcggcaac accaggctga
      241 actcctcggc ggaaaggcgc gcggcgtcgg agcgcatcgt cacctgctcc aggacgttgc
      301 gcagctcgcg gatgtttccc ggccacgcgt aggaggacag gaccgcgagc gcggtgggcg
      361 cgagctcgcg ctgcgggcgg ccgctgcgcc gcgcgatgtc ctcgaggaca tgctcgcaca
      421 gcgcctcgag gtcggaaatg cggtcgcgaa gcggcggcag ctcgatgggg agcacggaga
      481 gccggtagta caggtcggaa cggaagcgcc ccatgcccac catctggcgc aggtccacgc
      541 tgctcgcggc gatgacgcgc acgtccacct tcgccacgcg gttcgacccc agcggctcca
      601 cttccttttc ctgcagcacg cgcagcagct tcgcctgcat gctcatcggc atgtcgccga
      661 cttcatcgag gaacaacgtg ccgccgtggg cgagcatgaa cttgccgtcg cggccgcgac
      721 gatctgcgcc cgtgtaggcc gagggcgacg tgccgaagaa ctccgcctca aggagcgtct
      781 cggggacggc cgccacgttc acgccgatga acgggccgtg ggcgcggggg ctcgcggcgt
      841 ggatggcctg cgccagcact tccttgccgg tgccggtctc gccaagcagc agcaccgtgg
      901 tgtcggcctg cgcggcgcgc ctcgcctggc gcttcacctc catcgcgccc ggcgaagtgc
      961 cgatgaagtt ggagaaggtg tacttcacgc gccgttcctc ggcgaggctg cgccgcgtgc
     1021 tcgcgagctc gttctccagc tgctggaact tgcgcacgag cggctgcagc gaggccagcg
     1081 tgtcgaagag catgaatccc gccgcgccga tgacgcggcc acgctcgtcc ttcaccggca
     1141 cgcgcatcac cacgaaggac ttgtcgccga actgcatgat gtcgagcatc atcggctcgc
     1201 cggtgcgcac cacttcgcgc atgaggctgt tgggaatcac gtcctcgacg acgcgacccg
     1261 cagcctccgc aggggaggcg agcttgaggt gcgtcgcgta cttgtcgctg atccagacga
     1321 tgcgcgcgtc gcggtcgacg atgatggcgc cttcgcacat ggcgtcgagt ccctcgaaca
     1381 gggacttctt cgcgatccgc cggatggcgt cgtgatcctt gagccggggc atgggtctcc
     1441 tcccgccgcc ttgcgattgc gcgggcgcct tgcggcgccg gggcgctatt tcaccacgct
     1501 tttgtcctgc ccgaagagga tctttcgttc cttgtcggtg acggggccgc gcgggtccgg
     1561 gttcacttcc ttcgcctttg cataggcgcg cgtcgtgccg gggcgcgccc tgatggccgc
     1621 gtgccagcgc gcgacgttcg ggaaatcggc caggtcctgc ccctgccgct cgggaatgat
     1681 ccacgggtag gccgccatgt cggcgatcga atagtgcctg ccgaggatga agtcgcgtcc
     1741 cgcgaggcgc ttgtcgagca cgccgtagag ccggttcgtc tccttcacgt agcggtcgat
     1801 ggcgtaggga atcttctccg gggcgtagac ggcgaaatgg tggttctgcc cgagcatcgg
     1861 gccgagtcct cccatctgcc acatgagcca ctcgagcgcc tccacctgcc cgcgcaggtc
     1921 cttcgggatg aacttccccg tctttcccgc gaggtagagc aggatcgcgc ccgactcgaa
     1981 gacgctcagg ggcttgccgc cgtcagcggg ctcgtggtcg acgatcgccg ggatcttgtt
     2041 gttgggcgag atcttcagga acgcgggggc gaactggtcg cccttcgaga tgttgatccc
     2101 gtgcaggcgg tacggaagcc cggcctcctc gaggtacagg gtgatcttgt ggccgttggg
     2161 ggtggtccag tagtagaggt cgatcatggg gtctccttgc ggtggatggc gacgcggccc
     2221 ggcgcctggc acacgggcat gacctggatc gtattcacgt tcacgtgcgc gggccgggtc
     2281 gcggcccagt agacgcaatc ggcgatgtcc tcggcggtga gcggctcggt cccctggtag
     2341 acctgctgcg cgcgcgcctg gtcccccttg aagcgcacct ccgagaactc ggtgccggcc
     2401 accatgcccg gctgcacgtc ggtgaggcgc accgcggtgc cgaggagatc ggcgcgcacc
     2461 gacaggccga actggtgcac gaaggccttg gtcgcgctgt agacgtggcc gcccgcgtag
     2521 ggaaattccg ccgcgatcga gccgatgttg atgatgtggc cgcggttgcg cgcgaccatc
     2581 ccgtcgaggg ccgcgcgcgt gcagtagagg agccccttca cgttcgtgtc gaccatccgg
     2641 tcccagtcgt cgagattcgc cttctgcgcg ggctccaggc ccagggcgag gccggcgttg
     2701 ttcacgagga cgtcgacctc ggcgaacgcg ggcggcaggg ccttgaacgc ttccagcacc
     2761 gccgcgcggt cgcgcacgtc gagcacaacc gtgtgcaccg cccgccccag ttcctcgcga
     2821 agcgccgcga ggcgctcggc ccgccggccg gccgcgacga cgcggtgccc ctccttcgcg
     2881 aaccgccgcg cgatggcggc gccgaatccg gcggtggcgc cggtcacgaa tgcgatcatg
     2941 tcttcacctc cgctacgggg ccgggatcca ttttctacct gccgagaggc ccgacatcgg
     3001 cgcgcttgta ctggttgggc cagggcatcg cctcgtccag cgccctggcc gcgtgcctgg
     3061 cccagtacgg gtcgcgaagc agcgcacgcg cgaggcacac ggcatcggcc tgcccggtgg
     3121 caaggatgtg ctcggcctgc accggctccg tgatgagccc caccgcggcc gttgcgatgc
     3181 ccgcatcgcg gcggatggcc gcggcgaaag gcgcctggta cccgggaccg agcacgacct
     3241 tctggtccgc ggcatttccg ccgctcgaga catcgatcat gtcgacgccc agttccttca
     3301 gttcgcgcgc aaaggcgatc gactgctcga ggtcccagcc gccctccttc cagtcgctcg
     3361 cggagatgcg caccagcacg ggcttgtcct gcggccacgc gtcgcgcacc gcgcgcacca
     3421 ccgcgagcgg aaagcgcatg cgattggaga gggagccgcc gaagtcatcc ttgcgcaggt
     3481 tcgaaagcgg cgagaggaac tcgtgcaaca ggtacccgtg ggcggcatgc acctccgcca
     3541 cctcgaagcc cgcctgcagc gcccggtcgg cggccagccg gaagtcggcc acgcacgcct
     3601 cgagatccgg cgcggacatg gcccgcgggg ccgggtagtt gccgaaggcc acgtcgctcg
     3661 ggccgtgcgt cttccacccg ccggcgtcgc gggccagggg cccgccgccg ttccacggct
     3721 tgtcgcagga ggccttccgg cctgcatgcg cgagctggat gcccggcacc acgccctgcg
     3781 ccttgatgaa cgcggcgatc ggcttccagg ccgccgcatg ctgcgccgac cagatgcccg
     3841 cgtcccatgg cgtgatgcgg ccgtcgggcg aaacgctggc cgcctcggtg aatacgaggc
     3901 ccgtgccgcc gacggcgcgg ctgccgaggt gcacgaggtg ccagtcgttc ggcatgccgt
     3961 cctcgcacga gtactggcac atgggcgaga cgaagacgcg gttgggaatc gtgacggagc
     4021 gcagcgccac ggggctgaac aggagggaca tgggagtcat gcgggaaggg atttcgggag
     4081 ggccccaatc tagcacggcg gcgcgtgggc gaggcactcg ctcggctaga atcgcccgga
     4141 tggacgcgat cacgatacgc gccggcccgc gggcgctcgc ggcgattcgc gccaacggac
     4201 ttcgcgccgc cgatgtcgcg atcgtcccgg gcgcggcggg aggcccgaag gcgctgggcc
     4261 tgcacgggct ggacgtggcg atcttcggcg agtggttccc gaaggcgcct cgcgtgcgcc
     4321 acctcatcgg tgcgtccatc ggcgcgtggc gattcgccgc cgcgtgccgg cccgatgccg
     4381 ccgagggtct gcgcgagctt gcgcgcctat acatcgagca acgctacccc cctcgccccg
     4441 gtgccgcgct cgtcacccga aaggtgcgcg agatgctcgc cgagcttttc gacggtcacg
     4501 acgcggaaat cctcgcgagt cagggctacc gcctgcacat tctcgcggtg cggggccgcg
     4561 ggctcctctc gcgcgacgcg ggcatgcggc gcaccctcgg cttcggcgcc gccgtgttcg
     4621 ccaacctcgc ggggcgaagg cacctgcgcc acttcctcga gcgtacggta ttccacgacg
     4681 cgcgcgattg gccgtccttc ctcgacgacg cgaaggtcgc gggaagcctg ctgcccgcga
     4741 gcggcgccgc gcccatccgt ttcgatgcgt tccgcacgca cgccgtggcc ctcgaccgcg
     4801 acaacctgcg cgaggcgctt gtcgcctccg ccgcgatacc gatggtgatc gaggcggtgc
     4861 ccgacattcc gcgcgcaccg tcgggcgtgt actgggacgg gggcctcatc gactaccacc
     4921 tgcacctgcc gtactcacgc agcgacggcc tggtgctcta cccgcacttc acggatcgca
     4981 tcgttccggg ctggctcgac aaggcgttgc cgtggcggcg cgcgcgcgac gagtggctgg
     5041 acaacgtggt gctggtgtcg ccttcgcgcg agtacctggc ggcgctgccg tccggaaggc
     5101 ttcccgaccg cagcgacttc aggcgcttcg agtcggactt cgaggggcgc gtcgcccgct
     5161 ggcgaagggc catcgcggaa agcgcgcggc tgggcgacga gttcctcgcc ttcgcggagc
     5221 ggcccgaccc ggctcgcgtg ctgccgctgg aataaggggt cagattactt ttcggaaaaa
     5281 gggtcaggtt acttttctgt cttgttgccg tcgcgagacg gcggactgaa aagtaacctg
     5341 accctttttc cgaaaagtaa tctgacccct tattccaggg tgaggttgag cgcgcggccg
     5401 atgcgcgccc atttgcgcgt ctcggcgtcc acgacccgag cggcctcttc cggcgtggag
     5461 ccgaggggat cgatccactg cttgtcccag agcctcgcct tcagctccgg cgcgcggatg
     5521 atcttcgcga gctccgcgtg caggcgtttc actgcctcgg gcggcgtcct tgcggggacc
     5581 acgatcgcca tccacaccgg cgcctcgaag cccggaaagc ccgattcggc gaccgtcggc
     5641 aggtccggaa tcagcggtga gcgccgtgcg cccgtctgcg ccacgacgcg gatcctgccg
     5701 gcctgcacgt gcgcgatgag cgcagtggga ttgagcgaaa gcagttgcac gtcacccgcg
     5761 agcagcgccg ccaccgcggg agcgccgccg ttgtagggga tgtgcatcgt gtcgatgccc
     5821 gccgccgcgt tgagctgctc catcgaaagc tgcgagacgc tgcccttgcc gaccgaggcg
     5881 aaattgagct tgccgggctg cgccttcgcc ttggccacca cctccgcgag tgtggccgcg
     5941 cccagcgacg ggtgcacggc gatcaggctg ggggaggtgc ccacgagcgt cacgaacgcg
     6001 aaatcgcgcc ccacatcgta cgcaaggttc ttcgccagcg ccgggctcac ggcaagcggc
     6061 ccgttggtgc ccatgaggag cgtgtgcccg tcgggcgcgc tcttcgcgac ggcatcggcc
     6121 gcgatgttgc cgccggcgcc ggggcggttc tcgaccacca cgggctggcc cagcgctttc
     6181 gaaagcggtt ccgacagcgc gcgggcgatc acatccggct ggccgccggg cgcgaaggga
     6241 acgacgatcc tgaccggctt cgtcggttgc cagtccgcca gtgccgggag ggaaacgaca
     6301 atcaggacag caaggacgcc ccgcaagagc ctggtcattt cagatcccgg gggcgcggct
     6361 cactcgcgac ggcgccgcgt ccgatctcgc gctccccgca ttcccgaaga tgcttcgcca
     6421 gctcggccat gccctcgatc atctcgaggc gctcgcggac ggtgaatgac atccagagcc
     6481 ggatctgcgc gcggcggctg ccttcccacg tggtcaagct ccagtcgatt tcgtcaggat
     6541 ccggattcac ggctcttctc gtccaggatc atgcgcaggt gttccacgtc atccaggtcg
     6601 cgaggcctgc ccgccttgat tttcatgtcg ataagcgtcg ggatcgtgac gaagcgtacc
     6661 gagagccccg ggccgatttc ctggagaccg gcgcgcgcgt attccgcatc gaagtcgaag
     6721 ggctccgcca cgaagatgtc cacggtcgtg tcgcgataat gattgctgta cagattcagg
     6781 accagcattc ccttttccgt ccgaaagcgt tcccgctgcg cgccgtcagc gaactgttct
     6841 gccgtaatcg gcaccgtggg gcggaatccc gggcggtcga gcgcccgagc gatggcctcg
     6901 agagtggcga cgttcatgcc ggaagaatat cccctcggcg aattccggat gaacgaatca
     6961 gtaattgagc accggcgcca gccagcgctc gcacgccgcg atgtccatgc ccttgcggtg
     7021 cgcatagtcg gcaacctggt cgcgctcgat cttgccgacc gcgaaatagc gcgacgcggg
     7081 atgcgagaag tagaagccgc tcaccgcggc cgtcggatac atggcgaagg attccgtgag
     7141 cgcgatgccg gcgttcgtgt cggcctccag cagccggaag aggtcgccct tctccgtgtg
     7201 gtccgggcac gccgggtagc cgggcgcggg gcggatgccg gcgtacttct ccgcgatcag
     7261 gtcctcggcc gcaagctttt cgtccgccga gtagccccag aattcggtgc gcacccgatg
     7321 gtgcaggtgc tcggcgaaag cctcggccag gcggtcggcc agggccttga ggaggatcgc
     7381 ggaaaaatcg tcgtgccgcg cctcgaattc cttcacccgg gcctcgattc ccacgcctgc
     7441 ggtgacggcg aaggcgccga ggtagtccgc gatccccgag tccttcggcg ccacgaaatc
     7501 ggcgaggcac tggttggggt ttccggccgg cttctcgttc tgctggcgca ggttgtgcca
     7561 ggtcatcagc acgtgccggc gcgtctcgtc cgagtagatc tcgatgtcgt cgcccacgga
     7621 gttggcgggc catagtccca gcaccgcgtt ggccgtgagc cagcgcccct tcacgatgcg
     7681 ctcgagcatc gcccggccct cggcgtagac attgctcgcc gcttccccca ccacgacgtc
     7741 cgtgagtatg tcggggaact tcccggcgag gtcccatgac tggaagaatg ggccccagtc
     7801 gatgaagggc accagcttgt ccaggggata gttcttcagc gacaggagcc cgcggtgcct
     7861 cggcgccggc ggcgtgtagc cggcccagtc cagtttcacg gcgttggcgc gcgcggcctc
     7921 gaccggaatc gacttctgcc ccgccttctt cgcgtgggcg tcgcgcacct tcaggtagtc
     7981 gctgcgcacc tggctcacgt agcggtcgcg ctggtcttcg ctcaccaggc tcgccgccac
     8041 gcccaccgcg cgcgaggcgt ccggcaccca gacggtgggc cccgagtaat gcggctcgat
     8101 cttcaccgcc gtgtgcgtgc gcgacgtcgt ggccccgccg atgagaagcg ggatcctgaa
     8161 gcccagccgc tccatttcgc cggccaggtg cgccatttcc tcgagcgaag gcgtgatgag
     8221 gcccgagagc ccgatcatgt cggccttctc ccgcgtcgcc gtctcgagga tcttcgccgc
     8281 cgggaccatg acgccgagat ccaccacgtc gaagttgttg cactggagca cgacgccgac
     8341 gatgttcttg ccgatgtcgt gcacgtcgcc cttcaccgtg gccatgacca ccttgccctt
     8401 ggccttcgcg tcggcgctgc ccaggagcct cttctcctcc tcgatgtacg ggatgaggtg
     8461 ggccacggcc tgcttcatca cgcgcgccga tttcacgacc tgcggcagga acatcttgcc
     8521 ggcgccgaag aggtcgccga ccacgttcat gccgtccatg agcgggccct cgatcaccag
     8581 gatggggcgc gggaacttcc tcctcgcctc ctcggtgtcc tcgacgatcc actgcgtgat
     8641 gcccttcacc agcgcgtgcg acaggcgctg ctcgacgggg gcgtcgcgcc atgccagatc
     8701 ctcgacctgg tccttcttct ggcccttgaa cgattcggcg aaggtcacca ggcgctcggt
     8761 cgcatccggc cggcggtcga agatcacgtc ctcgacgcgc tcgagcaggt cggcggggat
     8821 gtccgcgtaa acgccgagct gcccggcgtt cacgatgccc atcgtcatgc cggccttcgc
     8881 ggcgtgatac aggaacgcgg tgtgcatggc ttcgcgcacc ggctcgttgc cgcggaagga
     8941 gaaggagagg ttggaaacgc cgcccgaggt cttcacgtgc ggcaggtgcc ggtggatcca
     9001 ggccagcgcc tcgaggtagt ccagcgcgta gcggttgtgc tcctcgatgc cggtggcgat
     9061 ggcgaagatg ttggcgtcga agatgatatc ctcggcggga aagcccacct gctccgtgag
     9121 gatccggtag cagcgctcgc agatcgactt cttgcgctcc agcgtgtcgg cctggccctt
     9181 ctcgtcgaag gccatcacga cgaccgccgc gccgtagcgc cgcgcgagct tcgcgtgctt
     9241 cacgaattcc gcctcgccct ccttgagcga gatggaattc acgatcgact tgccctgcac
     9301 gcacttgagg cccgcctcga tcacgctcca tttcgagctg tcgatcatga ggggcacgcg
     9361 ggcgatgtcg ggctcggccg cgacgaggtt gaggaatttc gtcatggccg cccgcgagtc
     9421 gagcatcgct tcgtccatgt tcacgtcgat gacctgcgcg ccgttctcca cctgctggcg
     9481 ggcgaccgcc agggcctcgg cgtagtcgcc ggcgaggatc aggcgtgcga aggccttgga
     9541 tccggtgacg ttggtgcgct cgcccacgtt cacgaagagg gaatcgtcgc cgatgttctg
     9601 cggctccagc cccgcaaggc gcagcttcgg ctcgatcttc gggatcgtgc gcggcctgca
     9661 cggcgagacg atgtcggcga tcgcgcggat gtgctcgggc gtcgtgccgc agcagccgcc
     9721 ggtgatgttg agccagccgg cctcggccca ttcgcggatg aaggcggccg tctcctcggg
     9781 caactcgtcg tattccgcca tcgcgttcgg cagccccgcg ttgggatgcg cggacacgag
     9841 cgtgtcggcg atgcgggcaa gctcctcgat gtgcgggcgc agctgcctgg cgcccagcgc
     9901 gcagttgaag ccgatcgaca gcggcctcgc gtggcgcacg gagttccaga acgcctccgg
     9961 cgtctgcccg gaaagcgtgc ggcccgaggc gtcggtgatc gtgccggaga tcatgatcgg
    10021 caggcgctca cccgtttgct cgaagcactc gtcgatggcg aagagcgcgg ccttggcgtt
    10081 cagcgtgtcg aagatcgtct cgaccaggat gatgtcggcg ccgccctcga cgagcgcgcg
    10141 cgcggcttcc aggtaggcgt ccttcacctg gtcgaaatcg acggcgcgaa accccgggtc
    10201 gttcacgtcc ggcgaaaggg agagcgtgcg gctcatcggg cccagcacgc cggccacgaa
    10261 tcgcggcttt cccggattct tcgcggtgta ttcgtccgcc acggcgcggg caaggcgggc
    10321 gccctcgcgg ttcagctccg cgacgatcgc ctcgaggtgg tagtccgcct gcgagatggt
    10381 cgtcgaattg aaggtgttgg tctcgaggat gtcggcgccc gcctccatgt acgccgcgtg
    10441 gatctcgcgg atcacctgcg ggcgggtgag cgtcaggagg tcgttgttgc cgcgcaggtc
    10501 gtggccgaag ctgcggaacc gctcgccgcg gtactcggct tccgtgagcc tgtagcgctg
    10561 caccatggtg cccatcgcgc cgtcgagcac gaggatgcgg gtggccaggg cgtcgtgaag
    10621 cgcctggatg cgggcggggc gtgtggcgag ggcttggtcc atggtcggcg gttccggaaa
    10681 cgaaaaaccc gtcagcgcag gcgacgggtt ttcgggccgg tcgctttagc tggatttatt
    10741 gagcgcccgc aagctgaagg atcaaatcgg cgctggagag cattctaccg ctccccatgg
    10801 ggaagggcaa tttggcggat ggcgaaccgc cgcgggtcga ttgcggaaga tcgcgcggcc
    10861 tagccggcgg ctcagaacgt gcgtattccg gggagctcgc cggcgtactc gtgccgctgg
    10921 cccgagtcgt cgtaccagta gagccggttc ggttcctcgc ccttggtgag cttgtgcgag
    10981 ccgtcgcagt acggctggtt ccgggacagg ccgcaccggc agagaaactt ctcctcgccg
    11041 ccgaggacga gcttgagggg ttcggtgtgc gtgcgcttga cgatgcgtgc catcatggcc
    11101 tccttcgttc agggactggt gcagttcgtt accggcgtgg aaaaaacctg ccgcacgatc
    11161 gccttcacct gcgtgacgcc gttcaccgtg tacgtgaagg tcccgttgtc gggatccgcg
    11221 aacgacagtg tcgccgtgcc gacctggtcg agggccacct tcgagccgtc ccacgtcgcc
    11281 gcggagaatg cgggacccgt cgtgtggtag agcgcgcccg agtaggtcgc ggccccggtc
    11341 ttcctgccgt ccgacatgac gagccacatg ccgcgacctg aagcgtcgta ggtgaaccac
    11401 gtgacgaaca gcgtatcggc ctggtggacg acgttgatcc cccatcccga ctcggagccg
    11461 gcaggcgcgc cccaccacag gtcctggtaa ttcggcgagg cgccgcggct gccgcccacc
    11521 atgcaattcg gcatgggcag cgcgtacacc tggcgcacga tgggcttcga ctgcatcgcg
    11581 ccgttcacgg tgtaggtgaa ggtgccacgg tccgcgctgg tgaaggcgaa gttggccgtg
    11641 ccgaccggcg tgaccgtcac catggccggg tcgaagtgcg ccgaatcgaa tgccggcccc
    11701 gtggttcgat agagattgcc cgtgtaggac ttcgtgttcg gatccatcat gccgtagccg
    11761 cccatgccgt aaccgccgta gtacggatcc atctcgatgg aaatgtcccg ccggccctcg
    11821 gacatcacca gccacagccc ggtgccgtcc gtgtcgtagg tgaaccacgt ggcgaacagg
    11881 atgtcgccct ggtggacgat gttcacgccc cagcccgatt cggagccggc gggcgagcgc
    11941 caccataacc cctggtagct catcgccggg tactgggcgc cggccggtgt tggcgcgaag
    12001 aggaacgcga gggagaccag ggaggcgagg agcgatcgaa ccgcatgcat ggtgacctcc
    12061 ttccggaccg gggagctgat ccgcaacggg gaccgggggc cggacttgcc gtgagagggt
    12121 aaacagcggg cgagtccagg gcattccctg ccccgacgac attccgcccg gcgccctggg
    12181 ttccggcgca cctgattgtc cttgcgaact gcagggatat cggcgtccgg aagaaattgc
    12241 cggcgcggtg gcgatgatcc ggcgaatcgc gccgtccggc aagcgccggc gcgccgcgtt
    12301 gcgctcaggc tggcggcttg cctttcaccg actgtgcggc ctcggatgcc gcgtgcgcca
    12361 tcctgacggc ttccgccgcc gcttccgccg cccgctttgc cgccagggtc gcatgggccg
    12421 aggcctgcac cgacacttcc tcggcctgtt gcgcggccgc ggccgacgcc gcagcggctg
    12481 ccgctactac cgtctcgttc gccgcttcgc gcgccgccgt cgcggcattc gttgccgcgg
    12541 ttgcggacag aatggccgac gccgctgccc tttcagccgc ctccctcgcg tggtccgtca
    12601 gcactttcag cgattcgatc gattcggagt atcgcttgcc gacgacaagg tagccttcgc
    12661 gcaagttcgc gatttccttt tcgaattcgg ccagcttcgc atagatcttc gttgcttcct
    12721 ggtccatggg gatctcctcg gattcgccgc cagcgccagt ccggtggcta cccatcaacg
    12781 cggctcttgc gtcgatgacg cgcaaatcat tctcgcggga agaccagcgc ccggcttgac
    12841 gcccctcaaa cccttccccg gcgaatcccc catgatgtcg cccatcctcg cacggagcat
    12901 tccgacatga aacccgccat cgccctcgca accgtcctcc tcgccgccgc cggattcgcg
    12961 gcccaggcca tgccctcgca acaggcggca acgctgaagg gcgaggtcct cgaaaccctg
    13021 aacgtcgaga gctacaccta cctgcgcctc aagacccagg acggcgagac ctgggccgcg
    13081 gtcccgacga ctgccgtgaa gaagggcgcc caggtcacga tcggcaacac gatggtgatg
    13141 gagaacttcg aaagcaaggc gctgaagagg aaattcgacc ggatcgtgtt cggcgcgatc
    13201 gccgacccga acgccaagcc cgccgcgccg gccgggtcgc acggctcggc cccggtagcc
    13261 agcgtccccg tcgcgaaagt ggcgaaggca accggcccgg atgcaaagac cgtggccgaa
    13321 gtggtgaagg gcaaggcgtc gctcaagggc aagccggtgc tcgtgcgcgc gcaggtcgtc
    13381 aaggtctctt ccggcatcct cggcaagaac tggctgcacc tccaggacgg ctccggctcg
    13441 gcggcggaca gcacgaacga catcatcgtg acgacgacgg acaaggcggc cgtcggtgac
    13501 atcgtgaacg cgaagggcac cgtgcgcacc gacgtgaacg tgggctcggg ctacgcctac
    13561 gcggtcctca tcgaggacgc ggcgctgcgc aagtaggttg gcgttcgcct cgtgaaggcg
    13621 atcctgctgc tggccctgtg gatcggcgcg tggccggcag ccctcgccgc cacccccggc
    13681 gaggtgcccg tcggcggcac tctgcgcgaa gcgccgctct acggcctgac cctcgactac
    13741 cgcaggctct ccgaattccg cggcaagccg ctcgtcatca acgtgtgggc aagctggtgc
    13801 gggccgtgcc gcgacgagat gggctccctc gaccgcctcg cccgcaggca caacggccgc
    13861 ggcctcaact tcatcggcat ctccacggac gactacgcgg acaaggcgca ggctttcgtg
    13921 gcggacaccg gcgtgtcctt cgacaactac ctggacaaga acctgctgct cgagaccatg
    13981 ctgggcgcca accgcctccc cctcaccgtt ctggtggatg cgcggggcaa ggtgctgaag
    14041 aaggtttatg gcgcccggca gtgggacagc ccggagaaca aggcgctcat cgcgggaacg
    14101 ctgggcgtga agctcgactg acccggccgg cggcccgaca atcggtaacg ctgcgacagg
    14161 gcgtttcgtc gcacaatgga tcccctggtc gagggacatg ccacatgaga acctgggcat
    14221 tcttgccgct gtcgattctg ctcggagcct gcgccttctc gccaccggcg ccggagtcca
    14281 gccgcttcct ggccgaccaa ctgttcggcg ccccgtcgga gcgcgtcgac gccgggacgg
    14341 tcttcgcggc gagcgaatcg atgaagcgtt acctaagaga cgagatcgga atcctgcccg
    14401 gctccaaggg ccggcagcag gtgctcatcg agaagttgcg cagcaagggc cagctcaagc
    14461 tcgaatacga cgccgcgttc acgcgcaacg ccgcccaggc cttcgaggcg cgcaccggca
    14521 actgcctttc gctggtgatc atgacggctg ccttcgcccg ggagatgggc atcgaggtgc
    14581 gctaccagaa cgtgttcgtg gacgagacct ggagccggca gggcgacctc tatttttcca
    14641 tcgggcacgt caacctcacc ctcggccgca ggccgcccgc gctcggcacg cgcatcgacg
    14701 acggcgagca gctcaccatc gacttcatgc cgccccgcga cacgcgcggc atgaactggc
    14761 gggtgatcgg ggagaagacc atcctcgcca tgtacatgaa caaccgcgcc gccgagtcgc
    14821 tcaccgcagg gaagacggac gacgcctact ggttcgcgcg cgaggccatc ctgcaggacc
    14881 cggagttcat gaccccgtac aacacgctcg gggtcgtcta ccagcgcagc ggccatctcg
    14941 ggcaggccga gcggatcttc gcctacgcgc tggagcgcga accgcgcaac acccacgcga
    15001 tgtcgaacat ggctaccgtt ctcgcgggcc tgggccgcgt cgccgaagcg caggccctcg
    15061 aacgaaggct cgcgcaactg gagcccgagc cccccttctc caacttcaac cgaggcgtgg
    15121 cggccatgcg cgaaggcgat ttccgcgcgg cgcgggacct cttcgcgaag gaagtggacc
    15181 gcgatcccgg ttaccacgag ttccatttct ggctggcgct ggcgctggcc aacctcgggg
    15241 agacggagcg ggcgaagcgc cacctggccc tcgccatgga atacagcacc acgcgcaagg
    15301 accacgacat ctacgcggcc aagctcgacc gcatcaggtc atcgcgcctt cactagctcg
    15361 cgccgtatct cgcgcaattt caccttcacc ttcgccgcgt tcgcgttgcc gatgcggaag
    15421 agcaccttgc gcccggccga ctcggattgc agcgcggggt cggcatactc cgggagcacg
    15481 tgcggcaccg cgagccgaac cggcgcggcc ggttccggcg cggtgagcag gtggtcgatc
    15541 acctgcacca ggcgcgtgtt gaagtgaccc ttgggatagc ccagctccac atacgccttc
    15601 tggaagagcg gactgaagcg aacgtagacg gcgacgagct tcctcgcgtc cactttttcc
    15661 agcaggagca tccagggcgc gtagcgcgcc gcgttcccgg gcgcgatggc gaggctctcg
    15721 tccttgccgg tggtggccat gaggccgccc acaggcttca ggggactcag gcgcatggcg
    15781 aaggtcctgc gcggcaggtt gtcgatcgtc acgacgatgt tgcgcacgag gcgctccgga
    15841 acgaagaacc tcgccagggc gtcggcgccc agggggcccg ccagcgcgtc ctgcaattcc
    15901 ttgtcgctcg cgtcgagcgc cggcaagggc ttcgccggct ccgcctccgc gctctcgggg
    15961 accggataat gctcggcagg cgcggattcc ggagcggcgg cgggtttcac ctgcgcaacg
    16021 ggcggtggaa tcgtcggcgg ctgcgcagcc ggccagaaat accacgcggc cgcgacggcg
    16081 ccgagccccg caacgacgat cagtccgatc cggatcggcc ccatctcaca cccctttcat
    16141 cccgatgact tgcgattgga ggcggcaatg gcggccgagt tccggcgccg cgcccgcccg
    16201 tcaggacagc gcggcctttt tcgcctccag ctcctcccag cgcgcgagca gccgcgtgag
    16261 ctccccctcg atggccgcgt ggcgctcgtt gagcgccttc aggtccacgc tgcggtcctg
    16321 gtagatcgcc gggtcgccga gccggtccgc gagttccttc tgctcccgct ccagcgcctc
    16381 cagcttgccc ggcaattgct ccagctcgcg caattcgttg aatcccagct tggtcttgcg
    16441 ccgcggcgcg cgcgtgtcgg tcctgggcga ttcgtccgcg ccgccctcga ccggcggctt
    16501 ccgcgcgtcc cgtgacgccc ccgttcgcgc gcgctgccag tccccgtaac cccccgcgta
    16561 ttccttccac cggccctcgc cctcgaacgc gatggtctgc gtgaccacgt tgtcgaggaa
    16621 ggcgcggtcg tggctcacga ggaagagcgt gcccgcgtag tcctgcagca gggactcgag
    16681 cagttccagg gtctcgatgt cgaggtcgtt ggtgggctcg tccagcacca gcacgttggc
    16741 ggggcggctg aagaggcgcg ccagcagcag gcggttcctt tccccgccgg agagcgtctt
    16801 caccggtgca ttggcgcgtt cggggggaaa caggaaatcg ccaaggtagc cgatgacatg
    16861 ctttcgcccc ccgtcgatct cgatgaagtc gcttccgggc gagatcgtgt ccgcaagcgt
    16921 ggcctcctcg tcgagcgcgg cgcggaactg gtcgaagtag gcgacggcga ccttcgttcc
    16981 caggcgcacg ctgcccgcgt cgggggcgag ccgacccagg atcagtttga ggagcgtcga
    17041 cttgccgcag ccgttggggc cgatgaagcc gatcttgtcg ccgcgcagga tgcgcgtgga
    17101 gaacttctcc accacgcgct tttcgccgaa gcgcttggtg acgtcctcga gctccgtcac
    17161 gagcctgccg gagcgctccc ccggggaaag cgcgatgccg accttgccga cgcgctcgcg
    17221 ccgcgcggcg cgctccaggc gcagggcctc gaggcggcgc acccggccct cgttgcgcgt
    17281 gcggcgcgcc tccacgccct tgcggatcca cgcttcctcc tgcgccagca ccttgtcgaa
    17341 cttgcggttc accaccgcct cgacggcgag ctgctcggcc ttgcgcgcct cgtaggcgct
    17401 gaagttgccg ggatagtcgg ccaggcgccc gcggtcgagc tcgacgatgc gttgcgccac
    17461 gcggtcgagg aagcgccggt cgtgggtgac gaagagcacc gcgccggtga aggagacaag
    17521 ggtctcctcc agccactcga tggccgcgat gtcgagatgg ttggtgggtt cgtcgagcag
    17581 caacaggtcc ggggcgagca ccagcgcccg cgcgagcgcc acgcgcttct tcatgccgcc
    17641 cgaaagatcg tccaccatcg cgtcttcggg aagggcaacg cgcgtgagcg tggcctcgat
    17701 gcgatgctgc agcgtccagc ctccggcagc ttccagcgct tcctgcagct cgtgcaggcg
    17761 cgccatcagc gcctcctccg acgggccgcc gggatgcgcg gccagggcgt gcgtcgcatc
    17821 gtggtactcc acgaggagcc gcgtgccctc gcccaggccc tcggcgaccg cttcgaacac
    17881 cgtcagcccg ggcggaaaag cgggctcctg agggacgaat gcgagcttga ttccggatgc
    17941 gagccacacc ttcccgtcgt ccgcgcgccc cgtgccctcg atgatcttca ggaggctcga
    18001 cttgccggtg ccgttgcggc cgatgagccc gacgcgctcg cccgcctcga tcaccaggtc
    18061 cgcgtggtcg aggaggggca cgtgcccgta ggcgaggcag acctgggaga gctggaggag
    18121 gggcacccct gcattttccc atccggcggg gcggcgcgca cgggcgaagt gcgagtcggc
    18181 gtaaaattcg cggtgatccc ggcgcaaggc cggcccctcg caacgacgga aacccccatg
    18241 ccctccatca gccgccgcgc cgaccagctc ggcaccgaaa acgcgttcgt cgtcctcgcc
    18301 gaggtgagca agctgcaggc cgccggcaag gacatcatct cgttctgcat cggccagccc
    18361 gacttcccca cgcccgtgaa catccaggac gcggcggtga aggcgatccg cgcgggcaag
    18421 cacggctata ccccgtcggc gggcatcgcg gaactgcgcg aggccatcgc ggcctacgtc
    18481 tcgcgaaccc gcggcatttc cgtgagcgcg gatgacgtcg tcgtgggtgc gggggccaaa
    18541 cccttcatcg cctacaccat cctgtcggtg accgatcacg gcgcgggcga cgaagtgatc
    18601 taccccaacc ccgggttccc gatctacgaa tcgcagatcc gcgccatggg cgcggtgccg
    18661 gtgcccatct acctgcgcga gtcgcgcgga ttcaacttcg atccctcgga gctggaggcg
    18721 aagatcacgc cgcgcacgaa gctcctcatc ctcaattccc cgcagaaccc gaccgggggc
    18781 atcgtttcgg ggaaggacat gcaggccatc gcggccatcc tcgcccggca cccgaacgtg
    18841 tgggtatacg ccgacgagat ctacagccgc ctcgtctacg acggcgagtt cgcgtcgctc
    18901 gccgcgatcc ccggcatgca ggagcgaacg atcgtctgcg acggcgcctc gaagacctgg
    18961 gccatgaccg gctggcggct gggctacgcg gtgaacaagg cgctcgcccc cgtgttcacg
    19021 cgctgggtga cgaacaccga atcctgcgct tcccacatca gccagtgggc ggcggtggaa
    19081 gccatcaacg gcccgcagca cgacgcggag aagatgcgcg cgagcttcct cgcgcggcgc
    19141 gacctcatcg tggggctcct tacccaggtg ccgggcgtga cctgccagag tcccggcggc
    19201 gccttctacg cctggcccaa cgtgaccgag gcctgccgcc tggtgggcgc gaaggattcc
    19261 gaggagttcc gcgtgcggct gctgaacgag gccggcgtgg cggt
//
LOCUS       DASUEV010000271         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_755619, whole genome shotgun sequence.
ACCESSION   DASUEV010000271 DASUEV010000000
VERSION     DASUEV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755619"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1470)
                     /locus_tag="VFV55_08810"
     CDS             complement(133..1470)
                     /locus_tag="VFV55_08810"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX4944442.1"
                     /translation="MTPVLGRYKIVSEIGQGAMGTVYKAVDPIIDRTVAIKTINLNLS
                     KQELEEYEARFQQEIKAAGRLNHPNIVTIYDVGKTEQVAYMAMEFLEGQELKDIIASG
                     KLPPTEQTVDIIAQVADGLWFAHQQDIVHRDVKPSNIMVLKGGIAKITDFGIARLPNS
                     AVKTMTGLILGSPRYMSPEQVIGKSLDTRTDIFSLGVVLYEALTGIAPFDGDNVNAIM
                     YSTVNTAPPPPSTHNRQLPAMLDLIVAKAMAKAVDDRYQTVKEFADDLREARRQLDAA
                     RPSVALKARSAPPPSRPHGEATSQESPTIPLLTDGKHDKAAVREDESTKPLGIDKTFD
                     SFDATLRLAAMTNQIEEFEDYITATQKMRAYRGRIEAGSPLAPSVPAPAAEAEPAADQ
                     PKFRPIPRFVAPPSDSRDAGPAFPESRSTHLPAVIAIVVLAIVAIGLLVALLR"
     gene            complement(1467..2264)
                     /locus_tag="VFV55_08815"
     CDS             complement(1467..2264)
                     /locus_tag="VFV55_08815"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HEX4944443.1"
                     /translation="MRSLSGLRLLGAIAIAAMLAACAAIPPEAPKPVTPQITEDTLRA
                     RAKEQLAAGLKLYETGDYDGAQRSLQASLDHGLLGKTDQGMARKHLAFIHCVAGRASQ
                     CADEFRRAFEIDQAFALTPAEDGHPIWGPVYRNVRATLIAEREATQGKPKQALAKAEQ
                     MLFDGLVKYEAGEFPESLRLLEAAYKEGLKETADQVRALKHAAFCLCLLNRYPACRAE
                     FIKIYDVDPNFDLTPAEAGHPSWTRTFAGAKAQAKRALADKARKRVP"
     gene            complement(2264..>2942)
                     /locus_tag="VFV55_08820"
     CDS             complement(2264..>2942)
                     /locus_tag="VFV55_08820"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX4944444.1"
                     /translation="TSTNVKRAPTSTGTTTVTLTIPDFVQDKKKLTLVAVVLAGVVPV
                     LGFLVANKKGDPPLPAPVASTAPESATAATPAHVPEAPKAAPASGTSAGGRPETARPD
                     APKAEAGKPDAARPAGSAVPAKTEQAKGDATRSDAAKGDAAKGTSGSAPTAPAVPPAT
                     LVFSIQPWGEVYVNGKQSGVSPPVKSIKLAPGKYKVEIRNTTFAEHVENIEVKSRDEL
                     TIRHRFQ"
BASE COUNT          392 a          930 c         1072 g          548 t
ORIGIN      
        1 aaaggggtca ggttactttt cagcccgccg ccttgcgacg gcaatgcgac agaaaagtaa
       61 cctgacccct tttccgttca aagtaacctg accccttttc cgttcaaagt aacctgaccc
      121 cttttagcgc ttttagcgaa ggagggcgac gaggaggccg atggcgacga tcgccaggac
      181 gacgatcgcg atgacggcag gaaggtgggt gctgcggctt tcggggaagg cgggcccggc
      241 gtcccgggag tcggagggcg gggcaacgaa gcgcgggatg gggcggaact tcggctgatc
      301 cgcagcgggc tcggcttcgg cggccggcgc cgggacggag ggggcgagcg ggcttcccgc
      361 ctcgatcctg cctcggtacg cgcgcatctt ctgcgtggcg gtgatgtagt cctcgaactc
      421 ctcgatctgg ttggtcatcg ccgccagccg cagggtggcg tcgaaggaat cgaaagtctt
      481 gtcgatcccc agcggcttcg tggattcgtc ctcgcggacc gcggccttgt cgtgcttgcc
      541 gtcggtcagc agcgggatcg tgggggattc ctgcgacgtc gcctcgccgt gcgggcgcga
      601 cggaggcggc gccgagcgcg ccttcagcgc cacggagggc cgcgccgcat cgagctggcg
      661 ccgcgcctcg cgcaggtcgt cggcgaattc cttcaccgtc tggtagcggt cgtccacggc
      721 cttggccatg gccttggcca cgatgaggtc cagcatcgcc ggcaactggc ggttgtgcgt
      781 ggaaggcggc ggcggggccg tgttcaccgt ggaatacatg atggcgttca cgttgtcgcc
      841 gtcgaacggc gcgatgcccg tgagcgcctc gtagagcacc acgcccagcg agaagatgtc
      901 ggtgcgcgtg tccagcgact tgccgatcac ctgctcgggc gacatgtagc gcggcgagcc
      961 gaggatcagc ccggtcatcg tcttcacggc ggaattgggc agccgcgcga tgccgaagtc
     1021 ggtgatcttc gcgatcccgc ccttgagcac catgatgttc gagggcttca cgtcgcggtg
     1081 cacgatgtcc tgctggtgcg cgaaccacag cccgtcggcc acctgcgcga tgatgtccac
     1141 cgtctgctcg gtgggcggca gcttccccga cgcgatgatg tccttgagct cctgcccctc
     1201 gaggaactcc atcgccatgt aggcgacctg ctcggtcttg cccacgtcgt agatggtgac
     1261 gatgttcggg tggttgaggc gcccggcggc cttgatctcc tgctggaagc gcgcctcgta
     1321 ctcctcgagc tcctgcttgg agaggttgag gttgatcgtc ttgatggcga cggtgcggtc
     1381 gatgatcggg tccaccgcct tgtagacggt gcccatcgcg ccctgcccga tctcggagac
     1441 gatcttgtag cggccgagaa cgggggtcat gggaccctct tccgcgcctt gtcggcaagc
     1501 gccctcttcg cctgcgcctt ggcgcccgcg aaggtcctgg tccacgacgg atggccggcc
     1561 tcggcgggcg tgaggtcgaa gttcgggtcg acgtcgtaga tcttgatgaa ctcggcgcgg
     1621 caggcggggt aacggttgag caggcacagg cagaatgcgg cgtgcttcag cgcgcgcacc
     1681 tggtcggcgg tctccttcag cccctccttg tacgccgcct cgaggagcct gagcgattcg
     1741 gggaattcgc cggcctcgta cttcaccagc ccgtcgaaga gcatctgctc ggccttggca
     1801 agcgcctgct tgggcttgcc ctgcgtcgcc tcgcgctccg cgatgagggt ggcgcgcacg
     1861 ttgcggtaca ccgggcccca gatgggatgg ccgtcctcgg ccggagtcag ggcgaaggcc
     1921 tggtcgatct cgaaggccct gcggaattcg tccgcgcact gcgatgcgcg cccggccacg
     1981 cagtggatga acgcgaggtg cttgcgcgcc atgccctggt cggtcttccc gagcaggccg
     2041 tggtccaggg aagcctgcag gctcctctgc gcgccgtcgt agtcgccggt ctcgtagagc
     2101 ttgagcccgg cggcgagctg ctccttcgcg cgcgcgcgca gcgtgtcctc ggtgatctgg
     2161 ggcgtcacgg gcttgggcgc ttccggcggg atcgccgcgc aggccgccag catggcggcg
     2221 attgcgatcg cgcccaggag gcgaaggccg gagagggagc gcatcactgg aagcggtggc
     2281 ggatggtgag ctcgtcgcgg gatttcacct cgatgttctc gacgtgttcc gcgaaggtag
     2341 tgttgcggat ctcgaccttg tacttgccgg gtgcgagctt gatcgacttg accggcgggc
     2401 tcacgccgct ttgcttgccg ttcacgtaca cctcgcccca cggctggatc gagaagacca
     2461 gggtcgcggg aggaacggcg ggcgcagtcg gcgcgctgcc ggaagtaccc ttggcggcgt
     2521 cgcccttggc ggcgtcgctc ctggtggcgt cgcccttggc ctgctcggtc ttcgcgggca
     2581 ccgcggaacc cgcaggcctg gcggcgtcgg gctttccggc ttcagccttt ggtgcgtccg
     2641 gcctggccgt ctccggcctg ccgccggcgc ttgttccgga ggccggtgcc gctttgggag
     2701 cttccggcac gtgggcgggc gtcgcggcgg tcgcggattc cggcgcagtc gaggccacgg
     2761 gggcgggcag gggcggatcg cccttcttgt tggccacgag gaagccgagc accgggacca
     2821 cgccggcgag cacgacggcc acgagggtga gcttcttctt gtcctgcacg aagtcgggaa
     2881 tggtcagggt gacggtggtc gtgccggtcg aggtgggggc gcgcttcacg ttggtgctcg
     2941 tg
//
LOCUS       DASUEV010000272         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_644055, whole genome shotgun sequence.
ACCESSION   DASUEV010000272 DASUEV010000000
VERSION     DASUEV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644055"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /gene="hisG"
                     /locus_tag="VFV55_08825"
     CDS             complement(<1..116)
                     /gene="hisG"
                     /locus_tag="VFV55_08825"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831262.1"
                     /note="short form of enzyme; requires HisZ for function;
                     catalyzes the formation of N'-5'-phosphoribosyl-ATP from
                     phosphoribosyl pyrophosphate; crucial role in histidine
                     biosynthesis; forms heteromultimer of HisG and HisZ;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX4944445.1"
                     /translation="MITIALSKGRIYEETKPLLEAAGIVPAEDPEVSRKLILP"
     gene            complement(127..1377)
                     /gene="murA"
                     /locus_tag="VFV55_08830"
     CDS             complement(127..1377)
                     /gene="murA"
                     /locus_tag="VFV55_08830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX4944446.1"
                     /translation="MQKLSIQGGIPLSGEIRVSGAKNAALPILAAALLAGSPMRVANL
                     PRLNDVETMIALLARMGVRVSRDEGGASLDAGRITDPFAPYELVKTMRASILALGPLV
                     ARFGEARVSLPGGCAIGERPVDLHLKGLEAMGASVGIESGYIHAKAARLRGARIFMDT
                     VTVTGTENLMMAACLAQGETVLENAAREPEVVDLAQCLVAMGARIRGQGTDVIVVDGV
                     ESLRGVDYSVMPDRIEAGTFLAAAAATRGGITITHAPVAALDAVIEKLREAGAAITAA
                     GDSLSIRMDGRPRSVSVRTAPYPAFPTDMQAQFLALNTIADGAALVTETIFENRFMHV
                     QELRRLGAKIDIEGNTAVVHGVERLAGATVMATDLRASASLVIAGLVADGETVVDRIY
                     HLDRGYEHLEEKLSRLGARIRRVS"
     gene            complement(1421..1660)
                     /locus_tag="VFV55_08835"
     CDS             complement(1421..1660)
                     /locus_tag="VFV55_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEX4944447.1"
                     /translation="MADRRSIEETIRQGLECSHVEVRGDDGVHFEALIVSPAFAGLAR
                     VRRHQMVYAALGERMREEIHALSMQTLSPEEWAAR"
     gene            complement(1668..2426)
                     /locus_tag="VFV55_08840"
     CDS             complement(1668..2426)
                     /locus_tag="VFV55_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944448.1"
                     /translation="MTRGLPTLLRKEVMRFWKVGFQTVAAPVLLALLYLVIFSHALAS
                     HVDAYPGVGYTQFLVPGLAMMSMLQNAFANTSSSLIQSKVTGNIVFILLAPLTPGDIF
                     AAYLLASVVRAAVVGAGVILAVAWLVPLPLPNPLWLAAFAFTGAGILGALGIIAGIWA
                     EKFDQIAAFQNFVVMPLTFLSGVFYSVESLPPFWRAASHLNPFFYAIDGFRYAFFGKA
                     DVSPWTSLAIASATFCALSWLTLRLLRSGYKLRH"
     gene            complement(2423..>3044)
                     /locus_tag="VFV55_08845"
     CDS             complement(2423..>3044)
                     /locus_tag="VFV55_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944449.1"
                     /translation="SGYFGLRDNGAWIGELLERLALGPKADANMRSLSGGMKRRVMVA
                     QALVHRPPVIVLDEPTAGVDVELRQTLWQFVRELNASGHTIVLTTHYLEEAQHLCSRI
                     AMMKAGRIVALDSTRRLLATFSERVLRVKLEGCDLPASLAAGATLDAAGWHRIPVESA
                     AQVESALARLRESGARVAHLEVTEPELEEVFVKIMARHESPALEEA"
BASE COUNT          500 a         1062 c         1042 g          440 t
ORIGIN      
        1 ggcaggatga gcttgcgcga gacctcgggg tcctccgcgg gaacgatgcc cgccgcctcg
       61 agcagcggct tcgtttcttc gtagatgcgt cccttcgaca gcgcgatggt gatcatggcg
      121 gggctttcag ctcacacgac ggatgcgcgc gccgagccgg gagagcttct cctcgaggtg
      181 ctcgtagccg cgatcgagat gatagatgcg atccaccacg gtctccccgt ccgcgacgag
      241 gcccgcgatc accaggctcg cggaggcgcg caggtcggtc gccatcaccg tcgcgccggc
      301 caggcgctcc acgccgtgca ccaccgcggt gttgccttcg atgtcgatct tcgcgccgag
      361 gcggcgcagt tcctgcacgt gcatgaaccg gttctcgaag atcgtctcgg tcaccagcgc
      421 cgcaccgtcg gcgatggtgt tgagggccag gaactgcgcc tgcatgtccg tcgggaatgc
      481 cgggtacggc gcggtgcgca cgctcaccga tcgcggccgg ccgtccatcc ggatcgagag
      541 cgaatcgccg gcggcggtga ttgccgcacc ggcttcgcgc agcttctcga tcaccgcgtc
      601 gagcgccgcg accggggcgt gcgtgatcgt gatgccgccg cgcgtggccg ccgccgcggc
      661 gaggaacgtt cccgcctcga tgcgatcggg catcaccgaa tagtccacgc cgcgcaggct
      721 ctccacgccg tcgaccacga tcacgtcggt accctgcccg cggatgcgcg cacccatggc
      781 gaccaggcat tgcgcaagat cgacgacctc gggctcccgc gcggcattct ccagcaccgt
      841 ctcgccctgc gccaggcagg cggccatcat caggttctcg gttccggtga cggtgaccgt
      901 gtccatgaag atcctcgcgc ccctgagccg ggcagccttc gcgtggatgt accccgactc
      961 gatcccgacc gatgcaccca tggcctcgag acccttcaag tgcaggtcca cggggcgctc
     1021 gccgatggcg cacccgcccg gcagcgacac gcgcgcctcc ccgaagcggg ccaccagcgg
     1081 ccccagcgcg aggatcgagg cgcgcatcgt cttcaccagc tcgtagggcg caaaaggatc
     1141 cgtgatgcga cccgcgtcga ggctcgcgcc gccttcgtcg cgcgagacgc gaacccccat
     1201 gcgcgccaag agggcgatca tcgtctccac gtcgttgagc cgcggcaggt tggccacgcg
     1261 catgggcgag cccgccagca gcgccgccgc gaggatgggg agtgcggcgt tctttgcgcc
     1321 ggagacgcgg atttcgccgg acagcgggat accgccttgg atcgagagct tctgcatgga
     1381 tcgttctagg gggccggacg ggccggcccg gggaatcggt tcagcgggcg gcccactctt
     1441 cgggagaaag ggtttgcatg gagagcgcgt ggatttcctc gcgcatgcgc tcgccgagcg
     1501 ccgcgtagac catctggtgc cgccgcacgc gggccagccc ggcgaaggcc gggctcacga
     1561 tgagcgcctc gaaatgcacc ccgtcgtcgc cgcggacctc gacgtgcgag cactcgagcc
     1621 cctggcggat cgtttcctcg atggatcttc tgtcggccat ggcggcctca atgcctcaac
     1681 ttgtatccgg aacgcagcag gcgcagggtc agccatgata gggcacagaa cgtggcgctc
     1741 gcaatcgcga ggctcgtcca cggcgagacg tccgccttgc cgaagaacgc gtagcggaac
     1801 ccgtctatcg cgtagaagaa cggattcagg tgcgaggccg cccgccagaa cggggggagc
     1861 gactcgaccg agtagaagac cccggacagg aaggtcaggg gcatgaccac gaagttctgg
     1921 aacgcggcaa tctggtcgaa cttctcggcc cagatcccgg cgatgatgcc gagcgcgccg
     1981 aggattcccg ccccggtaaa ggcgaaggcg gcgagccaga gcgggttcgg cagcggcaac
     2041 ggcacaagcc aggccaccgc gaggattacg cccgcgccca cgacggccgc gcggaccacg
     2101 gaagcgagaa ggtacgcggc gaagatgtcg cccggcgtga gcggcgccag caggatgaac
     2161 acgatgttgc ccgtgacctt cgactggatg agcgacgagg aggtgttggc gaacgcgttc
     2221 tgcagcatcg acatcatcgc gaggcccggc accaggaact gggtgtagcc cacgccgggg
     2281 taggcatcca cgtggctcgc cagggcgtgc gagaagatca ccaggtacag gagcgccagg
     2341 agcactggcg ccgccacggt ctggaagccc accttccaga agcgcatcac ctccttgcgc
     2401 agcagcgtcg gcagcccgcg cgtcacgcct cctccagcgc gggcgattca tggcgcgcca
     2461 tgatcttcac gaacacctct tccagttcgg gctcggtcac ctcgaggtgc gcgacccgcg
     2521 cgccggactc gcgcaggcgc gcgagcgcgc tctccacctg ggccgcgctc tccacgggga
     2581 tgcggtgcca tccggccgcg tcgagggtgg cgccggccgc gagcgacgcc gggagatcgc
     2641 agccctcgag cttcacgcgc agcacgcgct cggagaacgt cgcgaggagg cgccgggtag
     2701 aatcgagcgc gacgatcctc ccggccttca tcatcgcgat ccgcgagcac aggtgctggg
     2761 cttcctcgag gtagtgcgtc gtgagcacga tggtgtgccc ggacgcgttc agctcacgga
     2821 cgaactgcca gagggtctgg cgcagctcca cgtccacgcc ggccgtcggc tcgtcgagga
     2881 cgatgaccgg cgggcggtga accagggcct gcgccaccat caccctgcgc ttcatgcccc
     2941 ccgagagcga gcgcatgttg gcgtcggcct tcgggcccag cgccaggcgc tcgagcagct
     3001 ccccgatcca cgcgccgttg tcgcgcagcc cgaagtaacc cgac
//
LOCUS       DASUEV010000273         5871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_408662, whole genome shotgun sequence.
ACCESSION   DASUEV010000273 DASUEV010000000
VERSION     DASUEV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408662"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /gene="secY"
                     /locus_tag="VFV55_08850"
     CDS             <1..189
                     /gene="secY"
                     /locus_tag="VFV55_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181005.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX4944450.1"
                     /translation="CLLPEFMRTQYNVPFYFGGTSLLIVVVVTMDFMAQLQAYLMSHQ
                     YESLLKKANFKGTGFPVR"
     gene            201..419
                     /gene="infA"
                     /locus_tag="VFV55_08855"
     CDS             201..419
                     /gene="infA"
                     /locus_tag="VFV55_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX4944451.1"
                     /translation="MAKEETIQMQGEVVETLPNAMFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLTKARITFRAK"
     gene            431..544
                     /gene="rpmJ"
                     /locus_tag="VFV55_08860"
     CDS             431..544
                     /gene="rpmJ"
                     /locus_tag="VFV55_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX4944452.1"
                     /translation="MRVQASVKKICRKCKIVRRKRVVRVICSDPRHKQRQG"
     gene            582..953
                     /gene="rpsM"
                     /locus_tag="VFV55_08865"
     CDS             582..953
                     /gene="rpsM"
                     /locus_tag="VFV55_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX4944453.1"
                     /translation="MARIAGINIPNHQHASIALTAIFGIGRARARLICDAAGVKQSAK
                     IKDLSDGEMDKLREQVSRFGVEGDLRRETSMNIKRLMDLGCYRGVRHKKGLPVRGQRT
                     RTNARTRKGPRKAVRLSKAAA"
     gene            983..1375
                     /gene="rpsK"
                     /locus_tag="VFV55_08870"
     CDS             983..1375
                     /gene="rpsK"
                     /locus_tag="VFV55_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX4944454.1"
                     /translation="MAKPAATRVRKKVKKNVSEGIAHIHASFNNTIVTITDRQGNALS
                     WATSGGAGFKGSRKSTPFAAQVAAEAAGRAAQECGVKNLEVRIKGPGPGRESAVRALN
                     ALGLKILSIADVTPVPHNGCRPPKRRRM"
     gene            1412..2032
                     /gene="rpsD"
                     /locus_tag="VFV55_08875"
     CDS             1412..2032
                     /gene="rpsD"
                     /locus_tag="VFV55_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX4944455.1"
                     /translation="MARYTGPKCKLARREGTDLFLKSARRGLDTKCKLDVRPGQHGAK
                     MTRVSEYGHQLREKQKIRRIYGVLERQFRNYFEKAAQRKGPTGETLLQLLESRLDNVV
                     YRMGFASTRAEARQLVSHKAIQVNGKPVNIPSYLLRASDTVAVREKSKVQLRIKSSLE
                     LAEQNGFPSWVEVDSKNFKGVFKSVPDRAEFASDVNEQLVVELYSK"
     gene            2097..3074
                     /gene="rpoA"
                     /locus_tag="VFV55_08880"
     CDS             2097..3074
                     /gene="rpoA"
                     /locus_tag="VFV55_08880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX4944456.1"
                     /translation="MQSNATNFLKPRIIDVQNLSPTHAKVVMEPFERGYGHTLGNALR
                     RILLSSMPGYAATEVKITGVLHEYSTIEGVQEDVVDVLLNLKGIVMKLHGRGEVSLKL
                     KKDKPGPVTAADIEQTHDLEIINPDHVIAHITQGGKLEMSVKVERGRGYVPATSRRTE
                     DSRAIGAILLDASFSPVRRVSYQVESARVEQRTDLDRLVIDIETNGVIEPEEAVRYAA
                     SVLVDQLSVFASLQSTSEERIENAQPQVDPILLQPVDDLELTVRSANCLKAENIYYIG
                     DLIQRTENELLKTPNLGRKSLNEIKEVLASKGLTLGMKLENWPVAGLTK"
     gene            3169..3567
                     /gene="rplQ"
                     /locus_tag="VFV55_08885"
     CDS             3169..3567
                     /gene="rplQ"
                     /locus_tag="VFV55_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX4944457.1"
                     /translation="MRHRHGLRKLNRTSSHRLAMLRNMTVSLLKHEAIRTTLPKAKEL
                     RRVAEPIITLGKNPTLANKRLAFNRLRDRDTVVKLFDELGPRYKSRNGGYLRILKWGF
                     RQGDNAPMALVELMDRPEPKDEPAKEEKAA"
     gene            complement(3622..4914)
                     /locus_tag="VFV55_08890"
     CDS             complement(3622..4914)
                     /locus_tag="VFV55_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX4944458.1"
                     /translation="MTNPREILIGSFQAALAAADPLAIVKGHLPVPPAGRTVVVGAGK
                     AAAAMAAAVESNWPEGAALDGLVITRYGHGLPLRRVRVIEAGHPVPDEAGEAAAKEIL
                     ARASALGEDDLLLVLVSGGGSSLLSLPVEGIAMADLKAVTRDLLASGAPIQDMNTIRK
                     HLSAIQGGRLALASRARVLALVISDVTGDDPTHIASGPCSPDPTTYGDAIEILSRFGV
                     TAPASIAAHLAKGAAGQVPETPKPGDQGFNRVETRVIATAHASLMAAAAFVQRRGITP
                     MVLGDSVTGEAREVAKVHAAIARQLRLHSHPFKPPVAVISGGETTVTLRGNGKGGRCS
                     EFLLSLAIESGDLDHAWSLAADTDGIDGAEANAGAIVAPDTLRRAATLGLDAKKMLAN
                     NDGFGFFAALGDLVVTGPTLTNVNDYRIILVLQPSTHA"
     gene            complement(4911..>5871)
                     /locus_tag="VFV55_08895"
     CDS             complement(4911..>5871)
                     /locus_tag="VFV55_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX4944459.1"
                     /translation="PVGLLVCVTGVSGSGKSTLINDTLYASAARHLYGASTEPAEHEA
                     IDGLDHFDKVINVDQSPIGRTPRSNPATYTGLFTPIRDLFAGTPESRARGYGPGRFSF
                     NVKGGRCEACQGDGVIKVEMHFLPDVYVTCDVCRGRRYNRETLDVRYRGRNVCEVLDM
                     TVEAARELFSAVPAIARKLQTLMEVGLGYVRLGQSATTLSGGEAQRVKLALELSKRDT
                     GRTLFILDEPTTGLHFHDIDLLLRVLHRLRDHGNTVVVIEHNLDVIKTADWVIDLGPE
                     GGDGGGRVIAQGTPEDIAATPGSFTGRFLAPVLVPAGRRKASA"
BASE COUNT         1070 a         1996 c         1933 g          872 t
ORIGIN      
        1 tgcctgctgc cggagttcat gcgcacgcag tacaacgtgc cgttctactt cggcggcacg
       61 tcgctcctga tcgtggtcgt ggttacgatg gacttcatgg cgcagctcca ggcctacctg
      121 atgtcgcacc agtacgaaag cctgctcaag aaggcgaact tcaagggcac gggcttcccg
      181 gtgcgctagg gaaaccggga atggcgaaag aagagacgat ccagatgcag ggagaggtcg
      241 tggagaccct cccgaacgcg atgttccggg tgaagctcga gaacggccac gtggtgctcg
      301 gccacatctc cgggaagatg cggatgcact acatccggat cctgccgggg gacaaggtca
      361 cggtcgagct gaccccgtac gacttgacga aggcccggat cacgttccgg gccaagtaag
      421 gagaagagtg atgcgagttc aggcttcggt taagaaaatc tgccgcaagt gcaagatcgt
      481 gcggcgcaag cgcgtcgtgc gcgtcatctg ctcggacccg cgccacaagc agcgccaggg
      541 ctgatccacc ggtacgcaca ggataaacgc aaggaatagc catggcccgt atcgccggca
      601 tcaacatccc gaatcaccag cacgcctcga tcgcgctcac ggcgatcttc ggcatcggcc
      661 gcgcgcgcgc gcgcctcatc tgcgatgcgg cgggcgtcaa gcagtccgcc aagatcaagg
      721 acctctcgga cggggagatg gacaagctgc gcgagcaggt gtcccgcttc ggcgtcgagg
      781 gcgacctgcg ccgcgagacc tccatgaaca tcaagcggct catggacctc ggctgctacc
      841 gcggcgtgcg ccacaagaag ggcctgccgg tcaggggcca gcgcacgcgc accaatgcgc
      901 gcacccgcaa ggggccgcgc aaggccgtgc gcctttccaa ggccgccgcc tgaccccatt
      961 cacgagctga ggaatccata gcatggccaa acctgccgcc acgagagtcc gcaagaaggt
     1021 gaagaagaat gtgtccgagg gaatcgcgca catccacgcc tccttcaaca acacgatcgt
     1081 aaccatcacc gaccgccagg gcaacgccct gtcgtgggcg acctcgggtg gcgcgggctt
     1141 caagggctcg cgcaagtcga cgcctttcgc ggcgcaggtc gccgccgagg ccgccgggcg
     1201 cgccgcgcag gagtgcggcg tgaagaacct cgaagtgcga atcaagggtc ccgggcccgg
     1261 tcgcgaatcc gccgtccggg cgctcaatgc cctcggcctc aagatccttt cgatcgccga
     1321 cgtgacgccg gtgccgcaca acggctgccg tccgccgaag cgccgccgga tgtaaccggc
     1381 cggcgagaac gaagagaaat aggaagacac catggcacgc tataccggac ccaagtgcaa
     1441 gctcgcccga cgcgagggca ccgacctgtt cctgaagagc gcccgacgcg gcctcgacac
     1501 caagtgcaag ctcgacgtcc gtcccggcca gcatggcgcg aagatgacgc gcgtgtccga
     1561 gtacgggcac cagctgcgcg agaagcagaa gatccgccgc atctacggcg tcctcgagcg
     1621 ccagttccgc aactacttcg agaaggccgc gcagaggaag ggcccgaccg gcgagacgct
     1681 gctgcagctc ctcgagagcc gcctcgacaa cgtcgtctat cgcatggggt tcgcctccac
     1741 gcgcgccgaa gcccgccagc tggtctcgca caaggcgatc caggtgaacg gcaagccggt
     1801 gaacataccc tcctacctgc tgcgcgcttc ggacaccgtc gcggtgcgcg agaagtcgaa
     1861 ggtgcagctt cgcatcaaga gctccctcga gctggccgag cagaacggct tcccgagctg
     1921 ggtggaggtc gattccaaga acttcaaggg cgttttcaag agcgtgcccg accgagcgga
     1981 gttcgcctcc gacgtgaacg aacagctggt ggtcgagctt tactcgaagt aagcgctgct
     2041 ccggaggcac ggcagccggc tcgctccctc aaccgacact ggcagaggcc caacgcatgc
     2101 aaagcaacgc cactaatttc ctgaagcccc gcatcatcga cgtgcagaac ctttccccca
     2161 cgcacgccaa ggtggtgatg gagccattcg agcgcggcta cgggcacacg ctcggcaacg
     2221 cgcttcgccg catcctgctg tcctccatgc ccggctacgc cgcgacggaa gtgaagatca
     2281 ccggcgtcct gcacgagtac tcgaccatcg agggcgtgca ggaggacgtg gtcgacgtgc
     2341 tcctgaacct caagggcatc gtgatgaagc tccacggccg cggcgaggtg agcctcaagc
     2401 tgaagaagga caagcccggc ccggtcacgg ccgcggacat cgagcagacc cacgacctcg
     2461 agatcatcaa tcccgaccac gtcatcgccc acatcaccca gggcggcaag ctcgagatgt
     2521 cggtgaaggt cgagaggggc cgcggctacg ttcccgcgac ctcccgccgg acggaggact
     2581 cgcgcgcgat cggcgcgatc ctgctcgacg cctccttcag cccggtccgt cgcgtgagct
     2641 accaggtgga gagcgcccgc gtcgaacagc gaacggacct cgaccgcctc gtgatcgaca
     2701 tcgagaccaa cggcgtcatc gagccggaag aggccgtgcg ctatgcggcg agcgtgctgg
     2761 tggaccagct ctcggtgttc gcgtcgctgc aatcgaccag cgaggagcgg atcgagaacg
     2821 cccagccgca ggtcgacccg atcctgctgc agccggtcga cgacctcgag ctcaccgtgc
     2881 gctccgccaa ctgcctcaag gccgagaaca tctattacat cggcgacctt atccagcgca
     2941 cggaaaacga gctcctcaag accccgaacc tcggccgcaa gtcgctcaac gagatcaagg
     3001 aagtgctcgc ctccaagggc ctcaccctcg gcatgaagct cgagaactgg ccggtggcgg
     3061 gtctcaccaa gtaaggcagg gccgcaatac cgccccggcg atccggggtt cctcaaccca
     3121 taacgacaac aaggccgcac aggccaagag aatcgaaggg aacacgaaat gcgtcatcgt
     3181 cacggacttc gcaagctcaa ccgcaccagc agccatcgcc ttgcgatgct gcgcaacatg
     3241 accgtctcgc tcctcaagca cgaggcgatc cgtaccacgc tcccgaaggc aaaggagctg
     3301 cgcagggtgg ccgagcccat catcaccctc ggcaagaacc cgacgctcgc caacaagcgg
     3361 ctggcgttca accgcctgcg cgatcgcgac acggtcgtca agctcttcga cgagctggga
     3421 ccgcgctaca agagccgcaa cgggggctac ctgcgcatcc tcaagtgggg ctttcgccag
     3481 ggcgacaacg cgccgatggc gctggtcgag ctgatggacc gtcccgagcc gaaggacgag
     3541 ccggccaagg aagaaaaggc cgcctgattt ccgcccgcgc tgcgcccttg cgacagaagc
     3601 cgaccccagg gtcggctttt ttcatgcgtg ggtggacggc tgcaggacga ggatgatccg
     3661 gtagtcgttc acgttggtca gggtgggtcc cgtgaccacc aggtctccga gagccgcgaa
     3721 gaacccgaat ccgtcgttgt tcgcgagcat cttcttcgcg tcgagcccga gggtggcggc
     3781 gcgccgtagc gtatccggcg cgacgatcgc cccggcgttc gcctccgcgc cgtcgatgcc
     3841 gtcggtgtcc gcggcgagcg accaggcatg gtcgaggtcg cccgactcga tggcgagcga
     3901 gagcaggaac tcgctgcacc ggccgccctt cccgttcccg cggagggtga cggtggtctc
     3961 gccgccggaa atgacggcca ccggcggctt gaacgggtgc gaatgcaggc gcagctggcg
     4021 cgcgatggcc gcgtgcacct tggccacctc ccgcgcctcg cccgtcacgg aatcgcccag
     4081 caccatgggc gtgatgcccc gccgctgcac gaatgcggcg gcggccatga ggctcgcgtg
     4141 cgcggtcgcg atgacccgtg tctcgacgcg gttgaacccc tggtcaccgg gcttcggcgt
     4201 ttccggaacc tgtcccgccg ctcccttcgc gaggtgggcg gcgatcgacg cgggcgccgt
     4261 gacgccgaat cgcgaaagga tttcgattgc gtcgccgtac gtggtcgggt ccgggctgca
     4321 cggccccgag gcgatgtgcg tggggtcgtc gcccgtcacg tccgagatca ccagggcgag
     4381 cacgcgtgcc cgcgacgcga gggcgagccg cccgccctgg atcgcggaga ggtgcttgcg
     4441 gatcgtgttc atgtcctgga tcggcgcgcc gctcgccagc aggtcgcgcg tgacggcctt
     4501 taggtcggcc atcgcgatgc cttcgacggg cagcgacagg agactcgagc ccccgcccga
     4561 aacgaggacg agcagcaggt cgtcctcgcc gagggcggat gcacgggcca ggatctcctt
     4621 cgccgctgcc tcgcctgcct cgtcgggcac cgggtgtccc gcctcgatca cgcggacccg
     4681 ccgcaaggga agcccgtggc cgtagcgcgt gatgaccagg ccgtcgagtg ccgccccttc
     4741 aggccagttc gattcgacgg ccgctgccat ggctgcggcg gccttgccgg cgcccacgac
     4801 caccgtcctg cccgccggcg gcaccggcag gtgtcctttc acgatcgcca gcgggtcggc
     4861 cgccgccagt gccgcctgga agctgccgat gaggatctcc cgcgggttcg tcatgcagag
     4921 gccttccttc tgcccgccgg aacgagcacg ggcgccagga agcgcccggt aaagctgccg
     4981 ggcgtggccg cgatgtcctc cggggtgccc tgcgcgatca cccttccgcc gccgtcgcct
     5041 ccctccggcc cgaggtcgat gacccagtcc gccgtcttga tgacgtcgag gttgtgctcg
     5101 atcaccacca ccgtgttgcc gtggtcgcgc aggcggtgca ggacgcgaag gagcaggtcg
     5161 atgtcgtgga agtgaaggcc cgtggtcggt tcgtcgagga tgaagagggt ccggccggtg
     5221 tcgcgcttgg aaagttccag ggcgagcttc acgcgctgcg cctcgccccc ggagagtgtc
     5281 gtggcgctct ggccgaggcg cacgtatccg agccccacct ccatcagcgt ctgcagcttt
     5341 cgcgcgatgg cgggcaccgc cgagaagagc tcgcgggccg cctccaccgt catgtcgaga
     5401 acctcgcaga cattgcgtcc ccggtatcga acgtcgagtg tctcgcggtt gtatcgcctg
     5461 ccgcggcaga cgtcgcaggt tacgtagacg tcggggagga agtgcatctc caccttgatg
     5521 acgccgtctc cctggcacgc ctcgcaacgg ccgcccttca cgttgaacga gaagcgcccc
     5581 ggcccgtagc cgcgggccct cgattccggc gtgcccgcga agaggtcgcg gatgggcgtg
     5641 aagagacccg tataggtggc ggggttggag cggggcgtgc gcccgatggg gctctggtcg
     5701 acgttgatca ccttgtcgaa gtggtccagg ccgtcgatcg cctcgtgctc ggcgggctcg
     5761 gtcgacgcgc catagaggtg ccgcgcggcg gaagcgtaga gcgtgtcgtt gatgagggtc
     5821 gacttgcccg acccggaaac gcccgtcacg cacaccagga gccccacggg a
//
LOCUS       DASUEV010000274         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_756486, whole genome shotgun sequence.
ACCESSION   DASUEV010000274 DASUEV010000000
VERSION     DASUEV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756486"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFV55_08900"
     CDS             complement(<1..960)
                     /locus_tag="VFV55_08900"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX4944460.1"
                     /translation="MLYRLARSALFQLDPETAHELALKSMCALGPAVALLGAGSDRGE
                     ERTVMGIRFPNPVGLAAGMDKNAQYLEPLAALGFGHLEVGTVTPRPQPGNARPRLFRL
                     VEHEAIINRMGFNNVGVEQLLANIGKSSFRGVLGINIGKNFDTPIERAADDYLACLDA
                     VYARATYVTVNISSPNTKNLRDLQSKERLDELLAALMAKRESLAARAGQVKPLALKIA
                     PDLDDEQIAAIAELAVKHRIDALIATNTTIGRAGVEGHRHAGETGGLSGRPVFALSTE
                     VLRKLSRLLAGRIPLIGVGGILTGADAKAKFDAGASLVQVYTGF"
     gene            complement(989..1711)
                     /locus_tag="VFV55_08905"
     CDS             complement(989..1711)
                     /locus_tag="VFV55_08905"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX4944461.1"
                     /translation="MAKLNDLPIANLQFYATAPYPCSYLPGRLARSQVATPSYLIDTE
                     TYGALVSAGFRRSGAFTYRPYCDHCQACVPVRVAAAEFEPNRSQRRAWARHATLEAHY
                     GELKFSHEHYALYRLYQGERHRGGGMDHDSREQYAHFLLQSNVATRLIEFREGDKLRM
                     VSIVDELADGLSSVYTFFDPLVKQASFGTWNILWQVHEAVRRGLPYVYLGYWIAQSRK
                     MAYKCEFRPIEGLSRGEWKRLR"
     gene            complement(1684..2400)
                     /gene="aat"
                     /locus_tag="VFV55_08910"
     CDS             complement(1684..2400)
                     /gene="aat"
                     /locus_tag="VFV55_08910"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX4944462.1"
                     /translation="MIPFLRPSDPFPPVSKAMRSPNGLLCAGADLSPQRLVDAYSHGI
                     FPWYSKGDPILWWSPHPRMVLFPDELKVSRSLRKAVARGLFETRFDTAFAEVMSECAA
                     PRDGQQGTWIVPEMVAAYVRLHERGFAHSVESWKDGALAGGLYGILLGRVFFGESMFS
                     RETDASKVALVKLVERVKALGVRVIDCQQATRHLASLGAREIPRREFAQLLAESIQYP
                     PSGSRWPATEEAWQSSTTSP"
     gene            complement(2416..2925)
                     /locus_tag="VFV55_08915"
     CDS             complement(2416..2925)
                     /locus_tag="VFV55_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4944463.1"
                     /translation="MPARYLIEPLAKGHDRSSFACGSEALDTYLRRQARLDGERGVSA
                     PFVLIEPPARTVLGYYTLSASAVDAGDLPEEFRKKLPRYPQMPVTLLGRPAVDRSRKG
                     KRLGELLLMDALHKSLDGAAEIAAIAVFVDAKDASAKAFYEHFDFIALPRSPRRLFLP
                     MQTIARLFA"
BASE COUNT          487 a          982 c         1080 g          478 t
ORIGIN      
        1 gaaaccggtg tagacctgca ccaggctcgc gcccgcgtcg aacttcgcct tcgcatccgc
       61 cccggtgagg atgcctccga cgccgatgag cgggatccgc ccggccagca ggcgcgaaag
      121 cttgcgcagc acctcggtcg aaagcgcgaa caccggacgc ccggaaaggc cgcccgtctc
      181 gcccgcgtgg cgatggcctt cgacgcccgc gcggccgatg gtggtgttgg tggcgatcag
      241 cgcgtcgatg cggtgcttga cggcaagctc ggcgatggcg gcgatctgct cgtcgtcgag
      301 gtccggggcg atcttcagcg ccagcggctt cacctggccc gcgcgtgcgg caagcgattc
      361 gcgcttcgcc atgagggccg cgagaagctc gtccagcctt tccttcgact gcaggtcgcg
      421 caggttcttc gtgttgggcg aggagatgtt caccgtcacg taggtcgcgc gggcgtacac
      481 cgcgtccagg caggccaggt agtcgtcggc ggcgcgctcg atgggcgtgt cgaagttctt
      541 gccgatgttg atgccgagca ccccgcggaa ggaggatttt ccgatgttgg cgaggagctg
      601 ctccacgccc acgttgttga accccatgcg attgatgatc gcctcgtgct ccacgagccg
      661 gaagaggcgc ggcctggcgt tgccgggctg cgggcgcggc gtcacggtgc cgacctcgag
      721 gtggccgaat cccagggccg cgagcggctc gaggtactgc gcgttcttgt ccatgcccgc
      781 ggcgaggccg acggggttcg ggaagcggat ccccatcacc gtgcgctcct cgccccggtc
      841 cgagcctgcg cccaagaggg cgaccgcggg cccgagggcg cacatcgact tgagcgcgag
      901 ctcgtgcgcg gtttcggggt ccagctggaa gagggcgctg cgggcgaggc ggtagagcat
      961 caaggcgtcc gggcggctcg gggcgggatc acctcaggcg cttccattcc ccgcgcgata
     1021 gcccctcgat ggggcggaac tcgcacttgt aggccatctt gcggctctgc gcgatccagt
     1081 agcccagata gacatagggc agcccccggc ggacggcttc gtgtacctgc cagaggatgt
     1141 tccaggtgcc gaagctcgcc tgcttcacga gcgggtcgaa gaaggtatag acggaggaaa
     1201 ggccgtcggc cagctcgtcc acgatggaaa ccatgcgcag cttgtccccc tcgcggaatt
     1261 cgatgaggcg cgtcgcgacg ttggactgca ggaggaaatg cgcgtactgc tcgcggctgt
     1321 cgtggtccat gccgccgccc cggtggcgct cgccctggta gaggcggtag agggcgtagt
     1381 gctcgtggct gaacttcagc tcgccgtagt gcgcttccag cgtggcgtgg cgcgcccagg
     1441 cgcggcgctg cgagcggttg ggctcgaact ccgccgctgc cacgcgaacc ggcacgcacg
     1501 cctggcagtg gtcgcaatag gggcggtagg tgaatgcgcc ggagcggcga aagccggccg
     1561 agacgagggc gccgtaggtc tcggtgtcga tgaggtagct gggcgtggcg acctgcgagc
     1621 gggccagccg cccgggcagg tagctgcacg ggtacggggc agtcgcgtag aactgcaggt
     1681 tcgctatggg gaggtcgttg agctttgcca tgcttcctcg gtggccggcc agcggctgcc
     1741 ggacggggga tattgtatcg actcggccag caactgcgcg aactcgcggc gcgggatctc
     1801 ccgcgcaccc agggaggcga ggtggcgcgt ggcctgctgg cagtcgatga cgcgcacccc
     1861 gagcgccttc acccgctcga ccagcttcac cagggcgacc ttggacgcat ccgtctcccg
     1921 cgagaacatc gactcgccga agaacacccg gcccagcagg atgccgtaga ggccgccggc
     1981 gagggcgccg tccttccacg actcgaccga atgcgcgaac ccccgctcgt gcagcctcac
     2041 gtaggccgcg accatctccg ggacgatcca cgtgccctgc tgaccgtcgc gcggggcggc
     2101 gcattcgctc atcacctccg cgaaggccgt gtcgaagcgc gtctcgaaga ggcccctggc
     2161 gaccgccttc cggagcgagc gcgacacctt gagctcgtcg gggaacagga ccatgcgcgg
     2221 gtgcggcgac caccacagga tcgggtcgcc cttcgagtac cacgggaaga tgccgtgcga
     2281 gtaggcgtcg acgaggcgct gcggcgatag gtccgcgccg gcgcacagca ggccgttggg
     2341 cgagcgcatc gccttcgaga cgggcgggaa gggatcgctc gggcgcagga agggaatcac
     2401 cagttcaccg ttcgcctatg caaacaggcg ggcaatcgtt tgcatcggga ggaacaagcg
     2461 ccgcggcgac ctgggcaggg cgatgaagtc gaagtgttcg tagaacgcct tcgccgatgc
     2521 gtccttcgca tcgacgaata ccgcgatggc ggcgatttcc gcggctccgt cgaggctctt
     2581 gtgcaaggca tccatcagga ggagttcgcc aagccgtttg cccttgcgtg atcggtccac
     2641 ggcagggcgg ccgagcaagg taaccggcat ttgcggatag cgcggaagct tcttccggaa
     2701 ttcctccgga aggtcgcccg cgtcgacggc agaagcggaa agcgtgtagt agccgagcac
     2761 cgttcgggcc ggaggctcga tcagcacgaa cggggcagaa acgccccttt cgccatccag
     2821 ccttgcctgt cgccgaagat aggtatccag cgcttccgac ccgcatgcga agctcgaccg
     2881 atcgtgcccc ttggccaggg gctcgatgag gtaacgcgcc ggcaattaca ctcccatcgc
     2941 cttgcgatac cgggcggcgg cgcggctcag gcgcttgccg ggctccgccg gcgcaagcaa
     3001 ggctttcagg aacacggctc gatcctc
//
LOCUS       DASUEV010000275         5566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_756713, whole genome shotgun sequence.
ACCESSION   DASUEV010000275 DASUEV010000000
VERSION     DASUEV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756713"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..703)
                     /locus_tag="VFV55_08920"
     CDS             complement(293..703)
                     /locus_tag="VFV55_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX4944464.1"
                     /translation="MGNVLDSFAGFDDFLAYLAVSLVLLACFTAIYVRITPHREIALI
                     REGNLAAAFSLSGALLGFIVPLASAIEYSVGLVDMAIWGAIALAVQVAAFVVVKLLIP
                     TITDDIAAGRQAQGFFLGALSLGVGLLNAACMTY"
     gene            complement(732..1856)
                     /locus_tag="VFV55_08925"
     CDS             complement(732..1856)
                     /locus_tag="VFV55_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX4944465.1"
                     /translation="MKREDLVPRIDWPRKVEELGFHFHSIDGVYWDERACYRFTSAEV
                     DKLELATGELQSRCIDAAQRVIERGDYARFRIPEAFVPLIEQSWNDDEKSLFGRFDLS
                     WDGSGEPKLLEYNADTPTALLEASVVQWYWLKDVFPAHDQFNSIHERLIERWKRMAQD
                     FPADHIVHFTCVSDSAEDQGNLDYLRDTATQAGIDARPIDIADIGWDGSLFRDLDERA
                     ITVLFKLYPWEWLVREEFGANLLARTVRVVEPAWKMLLSNKAILPILWEMFPDHPNLL
                     PAYFEPGKFATDFVKKPIYSREGANVSITAGGRTIEEPGEYGEEGYIWQGYHELPRFG
                     DNYTVIGSWIVGEEPAGIGIREDSSPITKNTSRFVPHHFT"
     gene            complement(1867..2229)
                     /locus_tag="VFV55_08930"
     CDS             complement(1867..2229)
                     /locus_tag="VFV55_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944466.1"
                     /translation="MLKRIRKSSSRVTLVLIGLAGLSGCADERRDVYASREDCLADWG
                     NKPEDCTPATQQRHAGRGFFYGPSYRHWSYGSGSASPSRSSSSSRSSSSSGSSRSFSS
                     SGSTSRGGFGSSGRSSAS"
     gene            complement(2231..3076)
                     /gene="speE"
                     /locus_tag="VFV55_08935"
     CDS             complement(2231..3076)
                     /gene="speE"
                     /locus_tag="VFV55_08935"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX4944467.1"
                     /translation="MSEKIFERLNDASGVWFDGTLVERRQTAFQLLEVWDTPELGRIF
                     RLDGYNMTSERDEFFYHENLVHPAAIAHPAPRRALVIGGGDGGSSEELLKHPSIERVH
                     MAELDPDVIEVSRTHFAKVHHGAFDDPRLKVSVGDGLAYLRATDTRYDLVVMDLTDPV
                     GPSAELYSPATFALARRAMAEGSALVLHIGSPFSHPERVRQTLANLREVFRVVAPYFL
                     HIPLYGTIWGFACASDSLDPRLLEPAEADRRVTARGVGDLQYYNGETHRAVFALPNYV
                     RRLVA"
     gene            complement(3146..3553)
                     /gene="speD"
                     /locus_tag="VFV55_08940"
     CDS             complement(3146..3553)
                     /gene="speD"
                     /locus_tag="VFV55_08940"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX4944468.1"
                     /translation="MKGLHIIADLYGCRNREMLASSAKLREICVAACKDVGLTVLGDH
                     FYQFAGLDATQVGGATGAVVFAESHLAVHTWPERDSATLDVYVCNVTCDNSDKAEKLY
                     ATLAASLQPDDVLMERVWRGRDFPLKKKRLAAV"
     gene            complement(3766..4722)
                     /locus_tag="VFV55_08945"
     CDS             complement(3766..4722)
                     /locus_tag="VFV55_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX4944469.1"
                     /translation="MKHATFRQLKIFEAVARHLSFSRAAEELHLTQPAVSMQVKSLEG
                     EAGLALFEQVGKKIHLTEAGAEVARFALEVASRMKDCEDTLAAIRGVSGGRLHLAVVS
                     TAKYFAPRLLAEFSRRHPGVTVKLSVTNREQVIGELQANQVDLAIMGRPPRGLDVDAT
                     AFAKHPHGIIAPPGHPLAGKRNLELSRLAGENFLIREPGSGTRMAMERVFADHGVKPA
                     SSMELASNETIKQAVMAGMGLSFLSLHTVGLELAAGRLVRLDVRGTPVTRDWHVVCRQ
                     GKRLSPLAERFRAFLTAEGAQLIAETTGVRVRAKATPTAGKT"
     gene            4857..>5566
                     /locus_tag="VFV55_08950"
     CDS             4857..>5566
                     /locus_tag="VFV55_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEX4944470.1"
                     /translation="MSKSYSAGVKEYRQTYWEPDYRVSDTDLLACFKITPQEGVSREE
                     AAAAVAAESSTGTWTTVWTDLLTDLEHYKGRAYRIEDVPGDDTCFYAFIAYPIDLFEE
                     GSVVNVFTSLVGNVFGFKAIRALRLEDVRFPIAYVMTCGGPPNGIQVERDKMDKYGRP
                     LLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFSFVAEAIR
                     KAEAETGERKGHYLNVTAP"
BASE COUNT          940 a         1835 c         1882 g          909 t
ORIGIN      
        1 tggtggtgaa gctcctcatt cccaccatca cggacgacat cgccgcgggc aggcaggcgc
       61 agggcttctt cctcggcgct ctctcgctcg gcgtcggtct cctcaacgcc gcctgcatga
      121 cctactaaag gaaaaggggt caggttactt tgcacaaaaa ataggcaaga cggaaaaggg
      181 gtcaggttac ttttctgtcg tattgccgtc gcaagacggc gggctgaaaa gtaacctgac
      241 cccttttccg ttttgcctaa tttttgtgca aagtaacctg acccctttgc cgtcagtacg
      301 tcatgcaggc cgcgttgagg agaccgacgc cgagcgagag cgcgccgagg aagaagccct
      361 gcgcctgcct gccggcagcg atgtcgtcgg tgatcgtggg aatgaggagc ttcaccacca
      421 cgaaggccgc cacctgcacg gcaagcgcga tcgcgcccca gatcgccatg tccacgagcc
      481 ccacgctgta ctcgatcgcg gaggcgagcg gcacgatgaa gccgagcagc gcgccggaca
      541 ggctgaacgc cgcggcaagg ttgccttcgc ggatgagcgc gatctcgcga tgcggcgtga
      601 tgcgcacgta gattgcggtg aagcaggcaa ggaggacgag cgagacggcc aggtaggcga
      661 ggaagtcgtc gaagccggcg aaagagtcga gcacgttgcc catgggcggt tcctttgcgg
      721 cgggtcggcc gtcaggtgaa gtggtggggg acgaagcggc tggtgttctt cgtgatcggc
      781 gaggaatcct cgcggatgcc gatgcccgcg ggctcctcgc ccacgatcca ggagccgatc
      841 acggtgtagt tgtcgccgaa ccgcgggagc tcgtggtagc cctgccagat gtagccctcc
      901 tcgccgtact cacccggctc ctcgatcgtg cgcccgcccg cggtgatgga gacgttggct
      961 ccttcgcggg agtagatcgg cttcttcacg aaatcggtgg cgaatttccc gggctcgaaa
     1021 tacgcgggca gcaggttcgg gtggtccggg aacatctccc acaggatggg aaggatggcc
     1081 ttgttcgaca agagcatctt ccacgcgggc tcgaccaccc gcaccgtgcg cgcgagcagg
     1141 ttcgccccga actcctcgcg gacgagccac tcccacggat agagcttgaa gagcacggtg
     1201 atggcgcgct cgtcgagatc gcggaaaagg ctcccgtccc agccgatgtc ggcgatgtcg
     1261 atcgggcgcg cgtcgatgcc ggcctgcgtc gccgtgtcgc gcaggtagtc gaggtttccc
     1321 tggtcctcgg cgctgtcgga gacgcacgtg aaatgcacga tgtggtcggc cgggaaatcc
     1381 tgcgccatcc gcttccagcg ctcgatcagg cgctcgtgga tcgagttgaa ctggtcgtgc
     1441 gcggggaaga cgtccttgag ccagtaccac tgcaccacgc tcgcctcgag cagcgccgtg
     1501 ggcgtgtcgg cgttgtactc gagcaacttc ggctcgccgc tgccgtccca cgagaggtcg
     1561 aagcggccga agagcgactt ctcgtcgtcg ttccaggact gctcgatgag ggggacgaac
     1621 gcctcgggga tgcggaagcg cgcatagtcg ccgcgctcga tgacgcgctg ggcggcgtcg
     1681 atgcagcgcg actgtaattc ccccgtggcg agctcgagct tgtcgacttc ggccgaggtg
     1741 aaacggtagc aggcacgctc gtcccagtac acgccgtcga tgctgtggaa gtggaaaccc
     1801 agctcctcca ccttgcgcgg ccagtcgatg cgggggacga ggtcctcgcg cttcatcgcc
     1861 gtccgctcaa ctggccgacg accggcccga cgagccgaat ccgccgcgcg aggtggagcc
     1921 cgacgagctg aacgaccggc tcgagcccga cgagctggac gaccggctcg agctcgaaga
     1981 gcgcgacggg gaggccgagc cggagccgta ggaccagtgc cggtaggagg gcccgtagaa
     2041 gaagccgcgc cccgcatggc gctgctgggt ggcaggcgtg cagtcctccg gcttgttgcc
     2101 ccagtcggcg aggcagtcct cgcgcgaggc atagacgtcg cgccgttcat cggcgcagcc
     2161 ggacaagccc gcgagcccga tcagcaccag cgtcacgcgg ctcgacgact tgcggatccg
     2221 tttcagcatc tcaggcgacg agccggcgca cgtagttcgg cagcgcgaac acggcgcggt
     2281 gcgtctcgcc gttgtagtac tgcaggtcgc ccacgccgcg cgcggtcacg cgccggtcgg
     2341 cctcggcggg ctcgagcagg cgcgggtcca gcgaatcgga tgcgcaggcg aacccccaga
     2401 tcgttccgta cagcggaatg tgcaggaaat agggcgccac cacgcggaag acttcgcgaa
     2461 ggttggcaag cgtctggcgc acgcgctcgg ggtgcgagaa gggcgagccg atgtgcagca
     2521 ccagggcgct gccttctgcc atcgcccgcc tggcgagcgc gaaggtggcc ggcgaataga
     2581 gctcggcgga aggcccgacg gggtcggtga ggtccatcac caccaggtcg tagcgcgtgt
     2641 ccgtcgcgcg aaggtacgca agcccgtcgc ccaccgacac cttcaggcgc gggtcatcga
     2701 aagcgccgtg gtgcaccttc gcgaaatgcg tccgggagac ctcgatcacg tcggggtcga
     2761 gctcggccat gtgcacccgc tcgatgctcg ggtgcttgag cagctcctcc gaggaaccgc
     2821 cgtcgccgcc gccgatcacc agcgcgcgcc gcggcgccgg gtgcgcgatc gcggccgggt
     2881 gcacgaggtt ctcgtggtag aagaactcgt cgcgctccga ggtcatgttg tagccgtcca
     2941 gccggaagat gcggcccagc tccggcgtgt cccacacctc caggagctgg aatgccgtct
     3001 gccgccgctc gacgagcgtg ccgtcgaacc acacgccgct cgcgtcgttg agacgctcga
     3061 agatcttttc gctcatccgg gatgcgcccc gatcgtgacc gcgcccgcct tcggccgtgc
     3121 cgcccggcgc gagccggctg gcccgttaca ccgccgcgag gcgttttttc ttgaggggga
     3181 agtcgcgccc gcgccagacc cgctccatca gcacgtcgtc gggctgcagg ctggcggcga
     3241 gcgtcgcgta gagcttctcg gccttgtcgc tgttatcgca ggtgacgttg cagacgtaca
     3301 cgtcgagggt ggcgctgtcg cgctcgggcc acgtgtggac cgccaggtgg gactcggcga
     3361 agacgaccgc gcccgtggcg ccgccgactt gcgtcgcgtc caggccggcg aactggtaga
     3421 agtggtcacc gagaacggtg agcccgacgt ccttgcaggc cgccacgcaa atctcgcgca
     3481 gcttggcgga ggaggccagc atttcgcgat tccggcagcc gtacaggtcg gcgatgatat
     3541 gcagtccctt cactccgtta cccccttttt tctggcgaat tcctgcaggt gacgggaccg
     3601 gccgccttcg gcttcgggcg ccggccgcgt tcagaaaacg cggcggcgcc cccggaccag
     3661 gggcggcgcg tgtaaccctt tttgcagaga tttacccaag cagtttcgtg gtggagacaa
     3721 agggtgaaat tatagcgtca ttctgccccc ggcgtccgga ttttttcaag tttttccggc
     3781 agttggcgtc gctttcgcac gcacgcgcac gccggtcgtt tcggcgatga gctgggcgcc
     3841 ctccgcggtg aggaaagcgc ggaatcgctc ggccagcggc gaaagacgct ttccctgccg
     3901 gcagaccacg tgccagtcgc gggtgaccgg cgtgccgcgc acgtccaggc gaacgagcct
     3961 gccggcggcc agctcgaggc cgacggtgtg cagcgagagg aagctcaggc ccatgcccgc
     4021 cattaccgcc tgcttgatgg tctcgttgct cgcgagctcc atgctgctcg cgggcttcac
     4081 gccgtgatcc gcgaagacgc gctccatcgc catgcgcgtc ccggaacccg gctcgcggat
     4141 gaggaagttc tcgcccgcta gccgggagag ctcgaggttg cgcttgccgg caagcggatg
     4201 cccgggcggg gcgatgattc cgtgcgggtg cttcgcgaac gcggtggcat ccacatccag
     4261 cccgcgcggc ggccggccca tgatggcgag gtccacctgg ttggcctgca gctcgccgat
     4321 cacctgctcg cggttggtga cggaaagctt caccgtgacg ccgggatggc ggcgcgagaa
     4381 ctccgccagg agcctcggcg cgaagtactt ggcggtgctc accaccgcca ggtgcaggcg
     4441 cccgccagag acgccgcgga tggcggccag cgtgtcctcg cagtccttca tgcggctcgc
     4501 cacctccagc gcgaagcgcg ccacctccgc gccggcctcg gtgaggtgga tcttcttgcc
     4561 cacctgctcg aagagggcga ggcccgcctc gccctcgagg ctcttcacct gcatcgagac
     4621 cgccggctgc gtaaggtgca gctcctcggc cgcgcgcgag aacgaaaggt gccgtgcgac
     4681 cgcctcgaaa atcttcagct ggcggaacgt ggcgtgcttc atgcgggcgt cctcccggat
     4741 ggggcgatcg tcacataaga catgcctgat ggtagccata aacatatgtg actgtcattg
     4801 atgcatgaac ggaagcagaa tccagtcatc ccgcgtaccg acccacggag gaaccgatga
     4861 gcaagtccta cagcgccggc gtcaaggaat accgccagac ctactgggag cccgactacc
     4921 gcgtcagcga caccgatctc ctcgcctgct tcaagatcac gccgcaggag ggcgtctccc
     4981 gcgaggaggc tgccgccgcg gtcgccgccg agtcctccac cggaacctgg accacggtgt
     5041 ggaccgacct cctcaccgac ctcgaacact acaagggccg cgcctaccgc attgaagacg
     5101 tgccgggcga cgacacgtgt ttctacgcgt tcatcgccta tcccatcgac ctgttcgagg
     5161 agggctcggt ggtgaacgtc ttcacctcgc tggtgggcaa cgtcttcggc ttcaaggcca
     5221 tccgggcgct gcgcctcgag gacgtgcgct tccccatcgc gtacgtgatg acctgcggcg
     5281 gcccgccgaa cggcatccag gtcgaacgcg acaagatgga caagtacggc cggccgctgc
     5341 tgggctgcac catcaagccc aagctcgggc tctcggccaa gaactacggc cgcgccgtgt
     5401 acgaatgcct gcgtggcggc ctcgacttca ccaaggacga cgagaacgtc aactcgcagc
     5461 cgttcatgcg ctggcgcgac cgcttctcgt tcgtggccga ggcgatccgc aaggccgagg
     5521 cggagacggg cgagaggaag ggccactacc tcaacgtgac cgcgcc
//
LOCUS       DASUEV010000276         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_999468, whole genome shotgun sequence.
ACCESSION   DASUEV010000276 DASUEV010000000
VERSION     DASUEV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999468"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..837)
                     /locus_tag="VFV55_08955"
     CDS             complement(184..837)
                     /locus_tag="VFV55_08955"
                     /inference="COORDINATES: protein motif:HMM:NF016900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caleosin family protein"
                     /protein_id="HEX4944471.1"
                     /translation="MSVSQAVPKSFKPSVSRSKPQGRGAGALPRTLKARSRTGDTTLQ
                     RHASFFDDNGDRAVDVAECTRGLKALGLPFGVAEAAALAIVAPLSIQTRGSLLALSID
                     IENIQKGKHDSDTGILDNRGRFNARRFDKVFGACSTVDRNGDKAFTATELTRMIAANR
                     KTLLGSLVSTIEWQVLLALAADTKAVERGKTVPALSVARMKSFYDGTLFYNLARAHA"
     gene            complement(1169..1534)
                     /gene="ssrA"
                     /locus_tag="VFV55_08960"
     tmRNA           complement(1169..1534)
                     /gene="ssrA"
                     /locus_tag="VFV55_08960"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1594..2289)
                     /locus_tag="VFV55_08965"
     CDS             complement(1594..2289)
                     /locus_tag="VFV55_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX4944472.1"
                     /translation="MNNAIRAVLFDLDGTLADTAPDMLRTVNAMRESRRLEPVALEVV
                     RPYVSSGARGMIGAAFGMRPEDAGYAALREEFLAVYAENLCVDTRLFPGMDELLVRLE
                     DRGLAWGVVTNKFEHLARSVIDGLGLASRAAVMVGGDTCARAKPFPDPLLHAAQSLAL
                     QPSSILYVGDDERDVRAARAAGMPVIAAAYGYLGDGPEPSRWGADGVVGSPVEIARWA
                     GLEAGPLRPHLQR"
     gene            complement(2322..3050)
                     /gene="ubiG"
                     /locus_tag="VFV55_08970"
     CDS             complement(2322..3050)
                     /gene="ubiG"
                     /locus_tag="VFV55_08970"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX4944473.1"
                     /translation="MSTDTPERAAGNVDPSELAKFGALAHRWWDPESEMFGPLHRMNP
                     LRLAHIDRLAGGLAGKRVIDVGCGGGILAESMAAKGAKVTGIDLGEKALKVAMLHRLE
                     SGVEVDYRLVSAEDAAEAEPGAYDVVTCMELLEHVPDPASAVRACAKLAKPGGLVVFS
                     TINRNAKSFLFAIVGAEYVLRLLPRGTHEYAKFITPSELAAHCRTAGLAMADITGMTF
                     NPLTRVFALGPDAGVNYFLAARRD"
     gene            complement(3047..4366)
                     /locus_tag="VFV55_08975"
     CDS             complement(3047..4366)
                     /locus_tag="VFV55_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEX4944474.1"
                     /translation="MREADTLIHAAWVAPVEPPGALLADHTVAIREGRIEALLPTANA
                     SGAFLAKAEVHLDRHLLIPGLVNLHGHAAMALLRGIADDLPLMTWLKDHVWPAEARHV
                     GDEFVHDGSLLAAAEMLRGGITCFNDMYFFPEATARAALRAGIRASLGVIAVEFPSAY
                     ASDAASYLHKGLATRDSYRGEDLLSFCLAPHAPYTVSDETLARIATLSEEIDAPVHTH
                     LHETVDEIAQGVAHHGVRPIERLRRLGLVGPRLIAVHAVHLEEAEIDLLAHEGASIAH
                     CPSSNLKLASGIAPLAACRARGITVGLGTDGAASNNRLDILGEMRTAALLAKGASGDA
                     RAVGAHEALRMATLDGARALGLDARIGSIVPGKFADLAAVELSSPETLPCFDPVSDLV
                     YAAGREHVTHVWVAGEARLTGRRLTGVDEDEIRAKAAWWRQRIRNPA"
BASE COUNT          722 a         1463 c         1518 g          770 t
ORIGIN      
        1 cgcaacgagg tcgcgcggat gaagcggctc gtcaaggaac ggcagatcaa gctcgacgag
       61 tagatccggc cgtcactggg taagagttca agaggccgga cgcgggttcg attcctgaaa
      121 tgggaatcga acccgcgttc gctattacgc tgcgagcggg gattcctgga gtccccctga
      181 cacctatgcg tgtgctcttg cgaggttgta gaacagcgtg ccgtcgtaga acgacttcat
      241 tctggcgacg ctcaaggcag gcaccgtctt gccgcgctcg acggctttcg tgtcggctgc
      301 cagcgcgagc aacacctgcc attcgatcgt cgacaccaac gaaccaagga gcgtcttgcg
      361 attcgcggcg atcatcctgg tgagttcggt cgcggtgaac gccttgtcgc cattccggtc
      421 gaccgtggag cacgcgccga acaccttgtc gaagcgcctg gcgttgaacc ggccccgatt
      481 gtccaggatg ccggtgtcgc tgtcgtgctt gcccttctga atgttctcga tgtcaatgct
      541 cagcgccagc aacgaccctc gcgtctggat gctcaaggga gcaacgatgg cgagcgcggc
      601 ggcctccgcc acgccaaacg ggagtccgag agccttgagc ccgcgcgtgc actccgcaac
      661 gtcgacggcg cgatcgccgt tgtcatcgaa gaaggacgca tgccgctgca gcgtggtgtc
      721 gccagtccgg ctgcgcgctt tcaacgtgcg aggcagcgca cccgcccccc ggccttgagg
      781 tttgctgcgg gaaaccgacg gcttgaagga cttcgggacg gcttgggaaa cgctcatggt
      841 gggttcgaat ccgaacaggg gtgaatggcg atggatagcc agggattgtt actcttgcca
      901 acgtctgaca tgtgcgggcg acaactggct tgcggtcaca cgctcgatcg cgtaagcgcc
      961 agcgcgcagc ggcaaccgtt cgcttacgtt acgccgagac acggccggca tctgtgcggt
     1021 ggagaacatc gccttgcttt gctttagggc tcgttatggc ttcggcgtcc tcgccgtggc
     1081 agagcgactc cgacctcgtt tggcagtgct tcttccggag gtttacgaaa aaaaaagacc
     1141 aaccgcagat gcggttggtc tcgggtactg gtggaggcgg cgggaatcga acccgcgtcc
     1201 aaatgccatt ccacaaacag ttctacatgc gtagccggtt gttctgcttt agcgagtcgg
     1261 ccgcgaacca gctcccggcc tttcccacga gccacctatt ggtttaggtc ttcaccaagt
     1321 gacgcgatga attccgattc catgtttatg actccgctgc cggtgaactg gcccaaccca
     1381 tggaaaaact ggtgcggagc tggccggtat taagcggcca gggcgtacgt ttcgtcgttc
     1441 gcagttaagt ttgcgattcc agcggtttta cgaggcgttg gcgctcggca tgccctgcct
     1501 gcttcactgc acctgtcgaa accaggtcgc ccccggaaat cggggtcttg caacatccaa
     1561 ttttacgcgc ttgtgtccct cttcggggcc acttcagcgt tgaagatggg ggcgaagggg
     1621 ccccgcttca aggccggccc agcgcgcgat ttcgacggga gagccgacca cgccgtctgc
     1681 gccccagcgc gaaggttcgg ggccgtcgcc gaggtagccg tacgccgccg cgatgaccgg
     1741 catgccggcc gcgcgggcag cccgcacgtc gcgctcgtcg tcacccacgt agagaatgga
     1801 gcttggctgc agcgcgaggc tttgcgctgc atggagaagc gggtcgggga atggcttggc
     1861 gcgtgcgcag gtatcgccgc cgaccatcac cgccgcgcgc gacgcgagcc cgaggccgtc
     1921 gatgaccgag cgcgccaggt gctcgaactt gttggtgacc acaccccagg ccaggccgcg
     1981 gtcctcgagg cgaaccagaa gctcgtccat cccgggaaaa aggcgcgtat ccacgcacag
     2041 gttctccgcg tagacggcga ggaattcctc gcgcagcgcg gcgtagcccg cgtcctcggg
     2101 acgcatgccg aatgcggcgc cgatcatgcc acgcgctccc gacgagacgt acggccttac
     2161 gacttcgagc gctaccggct cgagccggcg cgactcgcgc atcgcattca ccgtgcgcaa
     2221 catgtccggc gccgtgtcgg cgagcgtccc gtcgaggtcg aagagcaccg cccggatcgc
     2281 gttattcatg ccggttgggc ccgataccgg atcacgcgcg gtcagtcgcg ccgcgccgcg
     2341 aggaagtagt tcacgccggc gtcgggcccg agggcgaaca cgcgggtgag cgggttgaag
     2401 gtcatgccgg tgatgtcggc catcgcgagc cctgcggtgc ggcaatgggc cgcgagttcc
     2461 gagggcgtga tgaatttcgc gtactcgtgc gtgccacgcg gcaacaggcg aagcacgtac
     2521 tcggcgccga cgatcgcgaa gaggaaggac ttggcattgc ggttgatggt cgagaagacc
     2581 accaggccgc cgggcttcgc gagcttcgcg caggcgcgca ccgcgctcgc ggggtcgggc
     2641 acgtgctcga gcaactccat gcaggtcacg acatcgtatg ccccgggctc cgcctcggcg
     2701 gcatcctcgg ccgataccag ccggtagtcc acttccacgc cgctttccag ccggtgcagc
     2761 atcgccacct tgagcgcttt ctcgccgagg tcgatgcccg tgaccttcgc tccctttgcg
     2821 gccatcgatt cggcgaggat gccgccgccg cagcccacgt cgatcacgcg cttgcccgcg
     2881 aggccgcccg cgagccggtc gatgtgcgcc aggcgcaggg gattcatgcg gtgcaggggc
     2941 ccgaacatct cgctctccgg gtcccaccag cgatgggcga gggcgccgaa cttggccagt
     3001 tccgacgggt cgacgttgcc tgcggcgcgt tccggggtat cggtgctcat gcggggttcc
     3061 ggattcgttg ccgccaccag gcggccttgg cccggatctc gtcctcgtcc acgccggtga
     3121 ggcggcgccc ggtgagccgg gcctcgcccg ccacccagac gtgggttacg tgctcgcggc
     3181 ccgccgcata gacgagatcg gatacgggat cgaaacaagg gagggtttcg ggagaggaaa
     3241 gttccacggc cgcgaggtcc gcgaacttcc ccggcacgat cgacccgatc cgcgcgtcga
     3301 gcccgagcgc gcgtgcgccg tccagcgtcg ccatgcgcag cgcctcgtgc gctcccaccg
     3361 cgcgtgcatc tccgctcgct cccttggcga ggagggcagc ggtgcgcatc tcgccaagga
     3421 tgtcgagccg gttgttgctg gccgcgccat cggtgcccag tcccaccgtg atgcccctgg
     3481 cgcggcaggc cgccagcggg gcgatgccgc tcgcgagctt caggttcgac gacgggcaat
     3541 gggcgatcga ggctccctcg tgcgcaagga ggtcgatctc cgcctcctcg agatgcacgg
     3601 cgtggacggc gatgagccgc gggcccacga gccccagtcg ccgcagacgc tcgatcgggc
     3661 gcacgccgtg atgcgccacg ccctgggcga tctcgtccac ggtctcgtgc aggtgcgtgt
     3721 gcaccggcgc gtcgatctcc tcggagagcg tcgcgatcct cgcgagcgtc tcgtcgctca
     3781 cggtgtaggg cgcgtgcggg gcgaggcaga acgagaggag atcctcgccg cggtaggaat
     3841 cgcgcgtggc gagccccttg tgcaggtagc tcgccgcatc cgatgcgtag gcgctcggga
     3901 actccaccgc gatcacgccg aggctcgcgc ggatgccggc gcgaagggcc gcccgcgccg
     3961 tcgcctcggg aaagaagtac atgtcgttga agcaggtgat gccgccgcgc agcatttcgg
     4021 ccgcggcgag caggcttccg tcgtgcacga actcgtcgcc cacgtgccgt gcctccgcgg
     4081 gccagacgtg gtccttgagc caggtcatca acggcaggtc gtcggcgatt ccccgcagca
     4141 atgccatcgc ggcatgcccg tgcaggttga ccaggccggg aatgaggagg tgccggtcca
     4201 gatgcacctc ggccttcgcc aggaacgcgc cagacgcgtt cgcggtggga aggagggctt
     4261 cgatgcgccc ctcccggatg gcgaccgtgt ggtcggccag gagcgcgccc ggcggctcga
     4321 ccggggcgac ccaggccgcg tgaatcaggg tgtcggcttc tcgcatggcg gcgcaggaac
     4381 aaaaaacccc gccggggcgg ggttttctgc cgggatcgtc ggggcttact gcttgatgcc
     4441 cttgaactcg atgtccacgc ggcggttctt ctg
//
LOCUS       DASUEV010000277         8696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1121267, whole genome shotgun sequence.
ACCESSION   DASUEV010000277 DASUEV010000000
VERSION     DASUEV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121267"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="VFV55_08980"
     CDS             <1..1926
                     /locus_tag="VFV55_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX4944475.1"
                     /translation="RWRFTVRLKRPRGLANPHTFEFEPWALERGIRATGYVRAAPPPV
                     RLAEHEAGWPQSLHRLRGAIRDSMRASLGDARFAGVLVALAIGDQDSIAGADWEVFWR
                     TGVGHLVSISGLHVTMLASFAFAVTSFLWVRVPVLVLAVPVRKVGIVAGVSAAAAYTL
                     LAGYGVPAQRTLVMLATAAAGLLADRHASPSRVLAAAVLAVLLVDPWAVLVPGFWLSF
                     GAVGAIFFALALRTRPPGAVGGAVITQAAVTLVLWPLLAALFGEVSLVAPIANAFAIP
                     LVSLVIVPLTLAGALLHLPALLVPAHALMEAAMVPLGWLAGLPGAVAENAAAPMGVVA
                     CAVSGALLLLAPRGVPFRAAGAVLLLPLALFRAPAPAHGEAWIDVLDVGQGLAVVVRT
                     ATHALAYDAGPSWNAESDSGNRIVVPFLRGEGVRRLDGLVITHADDDHAGGALSVARS
                     REPPWILSTLPEDDERNLLAPVTRPCLAGTRWEWDGVAFEVLHPGPVAMRDRRRENDR
                     SCVIRVEAGAGSLLLAADIERQAEGELLEHTRERLRADALLVPHHGSRTSSTPGFIAA
                     VSPSVALISAGWRNRFRQPSPAVEARYRERGAAILRTDLLGALRVQLPAQAGLPVTVR
                     YQAERRRYWSDRNAPAP"
     gene            2062..2682
                     /locus_tag="VFV55_08985"
     CDS             2062..2682
                     /locus_tag="VFV55_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944476.1"
                     /translation="MPQVTRRSTLIRLGALALTPWLPGHANAQERAITPSLTEGPFYP
                     RSLPADRDADLTRVAGNGATAAGTPLEFSGCLVDRRGRPLAGRVIEIWHCDEYGQYHH
                     IGEPAGAGDPAFQGWGQAAADGEGRFAFRTIKPPPYPGRTPHIHFTVRDGGRRTLTSQ
                     AFFEGERGNERDSLYRGLGTDAKLVTMRLENAGAGQRGSLEVVLGV"
     gene            complement(2671..4008)
                     /locus_tag="VFV55_08990"
     CDS             complement(2671..4008)
                     /locus_tag="VFV55_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX4944477.1"
                     /translation="METHHVIRFLNDAIDLVGVNDHGHDKRVGLIAHAFCVRLGLPRK
                     VKERIFVAGLLHDCGVSSTYTHDHLVAEDQWGEVDRHCFRGSRLLASLDCLSHLATIV
                     RFHHTPWDTTDFAAIPEDTRFAANLVFLADRLDAWLAPGVRAREHALAKAMAAMGTLF
                     FEAFAPAIASIARDPVFWADIGRARVGQNVDRILSNIEFPPLSQKDLRTVPFLMAAIV
                     DAKCPYTAEHSFRVAAIARRMGEVFALSGAECDDLETAGLLHDIGKLTIPDEIINKPG
                     PLTENERQVMETHAAHSYDVILHLPSLERIAELARHHHERGDGRGYPDGIGGDALSLS
                     ARIISAADFFQALTQRRPYRPGMDPSQVREALDEARRAGRLDAGVLDSLETRFDEFWE
                     LSTGPLNISGLEPGLARLCQDPDSAPPPWTRSRSSSAETSREFALLDGPESNA"
     gene            4198..6327
                     /locus_tag="VFV55_08995"
     CDS             4198..6327
                     /locus_tag="VFV55_08995"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX4944478.1"
                     /translation="MITEQSGADVAQLEAGLVTAFGAPLADSPLRALQLARGACQAAG
                     RDAAFARTVSVARLLLVQGADPETVAAALINGAIPEGELDPDGIQAAFGPELVALLKG
                     LGRAGRIEALATAGSDPEQLRKMLLAIAEDVRVVLIKLAERVVHMRSLTKADDALRRA
                     AGEETLALFAPLANRLGVSELKWELEDFAFRFTEPDLYRNIAGLLDEKRNDREAYIAR
                     VVAKLSEELAGMGIEATIHGRPKHLYSIFRKMHGKNLAFERLYDIRAVRVIVKNVREC
                     YAVLGVVHDLWTPVEGEFDDYIASPKANDYKSLHTAVVGPEGRTLEVQIRTDEMHQHS
                     EHGVAAHWRYKEGRRADQKFDAKIAWLRQVLAWKADVLAGEGAADRVPRGLFEETIYV
                     LTPQGRVVDLPAGSTPVDFAYHVHTDLGHRCRGARVDGQMVPLTTKLANAQRVEIIAA
                     KQGGPSRDWLSGNYLTSPRALAKVRQWFRHEDFAADVATGRGALEKELTRLGATSFAH
                     DRIAVSCGFARTEDFFAALGRGEITGRQIEIAVRGEVPVEPPPAVTVKAAPASRASSG
                     VLVLGVNNIATLVAKCCKPVPPDPIIGFITRTRGVMVHRLDCPNVTGLEPAQRERLLP
                     ADWGKTGESGFSADIEVVALDRQGLLRDISEAISRERINVTAVNTLSRGAHAFMRFTL
                     QVDGGDAIHRVLRQVRDVHGVDQARRL"
     gene            complement(7020..8543)
                     /locus_tag="VFV55_09000"
     CDS             complement(7020..8543)
                     /locus_tag="VFV55_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX4944479.1"
                     /translation="MNAATDIGGVCRDLGLDLAAVAGEGLRARSPVDGGELARLRADS
                     KEDVARIVAAAHAAFLAWREWPAPKRGELVRLFGEELRGAKESLARLVTLESGKILEE
                     ARGEVQEMIDICDFALGLSRQLHGLTIASERPGHRMMETWHPMGPTAVISAFNFPVAV
                     WAWNAALAWVCGNPVVWKPSEKAPLCALACRSLFERAVGKARGRLGDIPAGLGAVVLG
                     AREQGEWLVDDARIRIVSATGSTAMGRALAQRTSTRFVRTILELGGNNAMIVAPSADL
                     ELAVRAITFSAVGTAGQRCTSLRRLIVHESLRAPLVERLGKVFASLPIGNPLDKGTLV
                     GPLVDMPAFERMQEALRVARADGGAVLGGERVLAKEFPHAAYVRPALAAMPWQTAIVK
                     HETFAPILYVLGYRDLEEAIALHNDVPQGLASSIFTNDLREAEHFLSASGSDCGIANV
                     NIGPSGAEIGGAFGGEKDTGGGRESGSDAWKAYMRRATSTINFSGELPLAQGIRFDA"
BASE COUNT         1254 a         3059 c         3002 g         1381 t
ORIGIN      
        1 cgatggcgat tcacggtgcg gctcaagcgc ccgcgcggcc tcgccaaccc gcacaccttc
       61 gagttcgagc cctgggcgct ggaacgcggc atccgggcca cgggctacgt gcgtgccgcg
      121 ccgccgcccg tgcggctggc ggagcacgag gcgggctggc cgcagagcct gcaccggctg
      181 cgcggcgcca tccgcgattc catgcgcgcg tcgctgggcg atgcgcgctt tgccggcgtg
      241 ctcgtggcgc tcgccatcgg cgaccaggat tccatcgccg gcgcggactg ggaagtgttc
      301 tggcgcaccg gcgtcggcca cctcgtgagc atttccggcc tgcacgtcac gatgctcgcg
      361 agcttcgcct tcgccgtcac gtccttcctc tgggtgcgcg tgccggtgct cgtccttgca
      421 gtgcctgtgc gcaaggtggg aatcgtggcg ggcgtgtcgg cggcggccgc ctacacgctg
      481 ctcgccggct atggggtccc ggcgcagcgc acgctggtaa tgctcgccac ggccgccgcg
      541 ggcctcctgg ccgatcgcca tgcatcgcct tcgcgcgtac tggcggcggc ggttctcgcc
      601 gtgctgctgg tcgatccctg ggcggtgctg gttcccggat tctggctttc cttcggcgcg
      661 gtgggcgcca tcttcttcgc gctcgcgctg cgcactcgcc cgccgggagc cgtgggcgga
      721 gccgtgatca cgcaggcggc agtgacgctc gtgctctggc ccctgctcgc ggcattgttc
      781 ggcgaggtct ccctcgtcgc gcccatcgcg aatgcgttcg ccattccgct ggtgagcctc
      841 gtcatcgtgc cgctcaccct ggccggggcc ctgctgcact tgccggcgct gctcgtcccg
      901 gcccacgcgt tgatggaagc cgcgatggtg ccgctcgggt ggctggctgg gctgccgggc
      961 gccgtggcgg aaaacgcggc tgcgccgatg ggggtggtgg cctgcgcagt ttccggcgct
     1021 ctactgctcc tcgcgccgcg cggcgttccc tttcgtgcag ccggcgcggt cctgctgctt
     1081 cccctcgcgc tcttccgcgc gccggctccg gcccacggcg aggcctggat cgacgtgctc
     1141 gacgtggggc agggcctggc cgtggtcgtg cgcaccgcca cgcacgcgct cgcctacgac
     1201 gcgggcccgt cgtggaacgc ggaatcggac agcggcaacc gcatcgtcgt cccgttcctg
     1261 cgcggggagg gcgtgcggcg cctcgacggg ctcgtcatca cgcacgccga cgacgaccac
     1321 gcgggcggcg cgctctcggt ggccaggtcc cgtgaacccc cctggatcct gtccacgctc
     1381 cccgaggatg acgagcgaaa tctccttgca ccggtgacgc ggccctgcct cgcgggtacg
     1441 cgctgggagt gggacggtgt cgccttcgaa gtgctgcatc ccgggccggt ggcaatgcgc
     1501 gaccggcggc gcgagaacga ccgttcgtgc gtgatccgtg tcgaggcggg cgcgggctcg
     1561 ctgcttcttg cggcggacat cgagcggcag gcggagggag agcttctcga gcacacgcgc
     1621 gagcggcttc gcgccgacgc gctgctcgtc ccgcaccacg gctcgcgcac ctcttccacg
     1681 ccgggattca tcgcggcggt ttcgccctcg gtggcgctca tctcggcggg gtggcgcaat
     1741 cgcttccgcc aaccctcgcc ggcggtcgag gcgcgctatc gcgagcgcgg cgcggcgatc
     1801 ctgcgcaccg atcttctcgg cgccctgcgc gtgcagttgc ctgcgcaagc gggccttccc
     1861 gtcaccgtgc gataccaggc cgagcgccgg cgctactgga gtgaccgtaa cgcccccgct
     1921 ccgtgatgcg gggcggcgac gggtgaagcc gatcgcaatc ctgcgcaatt cgatgacgat
     1981 caggaacttt gccgggtcgt tcccctctga tcgtgatccg gggacgaagc tttcccggcg
     2041 ctcgccatcg aggagaaccc catgccgcaa gtcacccgcc gcagcaccct gatccgtctc
     2101 ggcgcactgg ccctcacgcc ctggctgccc gggcacgcga atgcgcagga gcgtgcgatc
     2161 acgccctcgc tcaccgaagg gccgttctac ccgcggagcc ttccggccga tcgcgatgcc
     2221 gacctcacgc gcgtggccgg gaacggcgcc acggcggcag gcacgccgct cgaattttcc
     2281 gggtgcctcg tggatcgccg cggccgcccg ctcgccgggc gcgtgatcga gatctggcac
     2341 tgcgacgaat acggtcagta ccaccacatc ggcgagccgg cgggcgccgg cgacccggcc
     2401 ttccagggct ggggccaggc cgccgccgac ggcgaggggc gcttcgcctt ccgcacgatc
     2461 aagccgcccc cgtatccggg gcgcacgccg cacatccatt tcacggtgcg cgatggcggg
     2521 cggcgcacgc tcacctcgca ggccttcttc gagggcgagc gcggcaacga gcgcgattcc
     2581 ctctaccgcg gactggggac cgacgcgaag ctcgtcacga tgcggctgga gaacgcgggc
     2641 gccggacagc gcggctcgct cgaggtggtg ctaggcgttt gattcgggac catcgagcaa
     2701 cgcgaactcg cgcgaagttt cggcgctgct ggagcgagag cgggtccatg gtggcggggc
     2761 cgaatccgga tcctggcaga gccgggcaag acccggctcg aggccggaaa tgttcagcgg
     2821 gcctgtcgaa agttcccaga actcgtcgaa tctcgtttcg agcgaatcga ggacgccggc
     2881 gtccagtctt cccgcccggc gggcctcgtc gagggcctcg cgaacctggc tcgggtccat
     2941 gccggggcgg taggggcggc gctgggtgag cgcctggaaa aagtcggccg cgctgatgat
     3001 cctcgcggac agcgacaacg catcgccgcc gatgccgtcc ggatagccgc gaccgtcccc
     3061 gcgctcatgg tgatggcgcg cgagttcggc aatgcgttcc agggacggta gatgcaggat
     3121 tacgtcgtag gaatgtgccg cgtgcgtttc catcacctgc cgctcgttct cggtcagcgg
     3181 gcccggcttg ttgatgatct cgtcgggaat ggtgagcttg ccgatgtcgt gcagcagccc
     3241 cgcggtttcc aggtcgtcgc attcggcgcc cgaaagcgcg aagacctctc ccatgcgccg
     3301 cgcgatcgcc gccacgcgaa acgagtgctc ggcggtgtag gggcatttcg cgtcgacgat
     3361 tgccgccatc aggaagggga cggttcgcag gtccttctgc gatagcggcg ggaattcgat
     3421 gttcgacagg atccggtcga cgttctgccc gacgcgggcg cggccgatat cggcccagaa
     3481 gacgggatcg cgcgcgatcg aggcaatggc cggcgcgaag gcttcgaaga agagcgttcc
     3541 catggccgcc attgccttcg ccaacgcgtg ctcgcgcgca cggacccccg gggcgagcca
     3601 cgcgtcgagg cgatcggcga ggaagacgag gttcgcggcg aagcgcgtgt cctccggaat
     3661 tgcggcgaag tcagtcgtgt cccaaggcgt gtggtgaaag cgcacgatcg tcgccaggtg
     3721 ggagaggcag tcgagcgacg cgagcaggcg cgagcccctg aagcagtgcc ggtccacctc
     3781 gccccactgg tcttccgcca ccaggtggtc gtgcgtgtaa gtcgacgaga cgccgcagtc
     3841 gtgcagcagc cccgcgacga agatccgctc cttcaccttc cggggcaacc cgaggcggac
     3901 gcagaacgcg tgcgcgatca gcccgactcg cttgtcgtgc ccgtggtcgt tgacgccgac
     3961 gaggtcgatg gcgtcgttca ggaagcggat gacgtggtgg gtttccatcg ggtgcccctg
     4021 ccctgtctat gattgctccg ccgggtccgt gaccgttgac gtggcgcaac aatgcgtcgg
     4081 ggaacgtgtg cacgggcgta catgttcttg ctcctttgtc ctggatggag cttgcgacca
     4141 atgctctcgg cctgcccgga ggtagaatcg gggctggaga cgaatccgac gccacgcatg
     4201 atcaccgaac aatcaggtgc cgacgtcgcg caactggaag ccgggctcgt cacggcattc
     4261 ggcgcgcccc ttgccgattc cccgctgcgg gcgctgcaac tcgcgcgagg cgcgtgccag
     4321 gccgcggggc gggacgcggc gttcgcccgg acggtctccg tggcgcggct ccttctcgtg
     4381 cagggcgccg acccggaaac ggtggccgcc gccctcatca atggcgccat ccccgagggc
     4441 gagctcgacc cggacggcat ccaggccgcg ttcgggccgg aactggtcgc gctcctgaag
     4501 ggattggggc gagccgggcg catcgaggcg ctcgccaccg cgggctcgga cccggaacag
     4561 ctgcgcaaga tgctcctcgc gatcgccgag gacgtgcgcg tggtgctcat caagctcgcc
     4621 gagcgcgtcg tccacatgcg ctcgctcacg aaggccgacg acgccctgcg ccgcgccgcc
     4681 ggcgaggaga cgctggcgct cttcgcgccg ctcgccaatc gcctcggcgt ctccgagctc
     4741 aagtgggagc tggaggactt cgccttccga ttcaccgagc ccgacctcta ccgcaacatc
     4801 gcagggctcc tcgacgagaa gcgaaacgac cgcgaggcgt acatcgcgcg cgtcgtggcg
     4861 aagctctcgg aggagcttgc cgggatgggc atcgaggcca cgatccatgg ccgccccaag
     4921 cacctctact cgatcttccg caagatgcac ggcaagaacc ttgccttcga gcggctctac
     4981 gacattcgcg ccgtgcgcgt gatcgtgaag aacgtgcgcg aatgctacgc ggtgctgggc
     5041 gtggtgcacg acctgtggac cccggtcgag ggggagttcg acgactacat cgcgagtccc
     5101 aaggcgaacg actacaagtc gctgcacacc gccgtcgtgg ggccggaggg aaggacgctc
     5161 gaggtgcaga tccgcaccga cgagatgcac cagcactcgg agcacggcgt ggccgcgcac
     5221 tggcggtaca aggaaggccg gcgcgcggac cagaagttcg acgccaagat cgcgtggctg
     5281 cgccaggtcc tcgcttggaa ggcggacgtg ctcgccgggg agggcgcggc ggatcgcgtg
     5341 ccgcgcgggc tcttcgagga gacgatctac gtgctcacgc cgcagggtcg cgtcgtggat
     5401 cttcccgcgg gctcgacgcc cgtcgatttc gcctaccacg tgcacaccga cctggggcat
     5461 cgctgccgcg gcgcccgcgt ggacgggcag atggtgccgc tcaccacgaa actcgccaac
     5521 gcgcagcggg tcgagatcat cgcggccaag cagggcgggc cttcgcgcga ctggctttcg
     5581 ggcaattacc tcacgagccc gcgcgcgctg gccaaggtgc ggcagtggtt ccgccacgag
     5641 gatttcgcgg ccgacgtggc cacggggcgg ggcgcgctgg agaaggagct cacgcggctg
     5701 ggcgcgacca gcttcgcgca cgaccggatc gccgtgtcct gcgggttcgc gcgcaccgaa
     5761 gacttcttcg ccgcgctcgg ccgcggcgag atcaccgggc ggcagatcga gatcgccgtt
     5821 cgcggcgagg tgccggtcga gccgcctcct gccgtcaccg tcaaggccgc gcccgcttcg
     5881 cgcgcgtcgt cgggcgttct ggtgctgggc gtgaacaata tcgccaccct cgtcgccaag
     5941 tgctgcaagc cggtgccgcc cgatcccatc atcggcttca tcacgcgcac gcgcggggtg
     6001 atggtgcacc gtctcgactg tcccaacgtc accgggctcg agcccgcgca gcgcgagcgg
     6061 ctgctgccgg ccgattgggg caagaccggg gaatcgggtt tctccgccga catcgaggtg
     6121 gtcgcgctcg accggcaggg gctccttcgc gacatctcgg aagcgatctc gcgggagcgc
     6181 atcaacgtga ccgcggtcaa cacgctctcg cgcggggcgc atgcattcat gcgtttcacc
     6241 ctccaggtcg acggcggcga cgcgatccat cgcgtgttgc ggcaggtgcg cgacgtgcac
     6301 ggcgtcgacc aggcgcggcg cctgtagcga ccttcgccgg cggcgtggga atgtcgatcg
     6361 caatcctggg cacgcaagct tgaccgtcgg atgggtgccc ggaaagtcga ggccaggcag
     6421 aacaagccag gcctcgactt tccacccatc tagagacgag ggcgctaacg ctttctcgtc
     6481 cgcgacggtc gcgtgccaag gattgcgctc gactttcccc gacgcctccc tccacgcatc
     6541 cgtggatgcg gcgtcgagat tgcacgcaac gcatttgcat cgcagttctg tcaacgcggt
     6601 gtcaaattac gcggcgcaac cgaatcgcgc ctccatcaac gctgtatcga acgttcgagc
     6661 agcctcgttg ggtgcgtccg cgaatgtacg agcattccca tctaacgcgt ctgtgacagg
     6721 ccgagtaacg tcactggctg cgttggcgac tgatcgaatc ggcacggcgc gtccactggg
     6781 gccggggatg gggcaagtct tccggcatcc ttggcacgcg accgtcggga tggggcagcg
     6841 tagcggcccc atctctcaat gggtaaaaag tcggggcctg gcttgttctg cctggccccg
     6901 actttttggg acccattcga cggtcacgat tgcgtgccca ggacgccgat taacttgccc
     6961 catccccggt cctcatccta attcgccgtg tgcatcaggc atcggaccgg cgacgcgtgc
     7021 taggcgtcga accggatgcc ctgggccaac ggcagctcgc cgctgaagtt gatcgtgctg
     7081 gtcgctcgcc gcatgtacgc cttccacgcg tccgaccccg actcccgccc gccgccggta
     7141 tccttctcgc cgccgaatgc cccgccgatc tccgcgccgc tggggccgat gttgacgttg
     7201 gcgatgccgc agtcgctacc ggacgcggac aggaagtgct ccgcctcgcg caggtcgttg
     7261 gtgaagatcg acgaggcgag gccctgcggc acgtcgttgt gcagcgcgat cgcttcctcc
     7321 aggtcgcggt agccgaggac gtagaggatc ggcgcgaagg tttcgtgttt cacgatcgcg
     7381 gtctgccacg gcatggcggc cagcgccggc cgcacgtagg cggcgtgcgg aaactccttc
     7441 gcgaggacac gctccccgcc cagcacggca ccaccgtcgg cgcgcgccac ccgcaacgct
     7501 tcctgcatgc gttcgaacgc cggcatgtcg acgagcgggc ccaccagcgt gcccttgtcc
     7561 aggggattgc cgatcggcag cgatgcaaac acctttccca gccgttcgac gagcggcgcg
     7621 cgcaggcttt cgtgcacgat gagacggcgc agcgacgtgc atcgctgtcc cgcggtcccc
     7681 acggcggaaa acgtgatcgc gcgcaccgcc agttcgaggt cggcgctggg cgccacgatc
     7741 atggcgttgt tgccgcccag ttccaggatc gtgcgcacga atcgcgtcga agtgcgctgc
     7801 gcaagcgccc gccccatggc cgtcgagccg gtggcggaga cgatcctgat gcgcgcgtcg
     7861 tccaccagcc actccccctg ctcgcgagcg ccgagcacga cggcgcccag gcccgcaggg
     7921 atgtcgccca gccgcccgcg ggccttcccg accgcccgct cgaacaggct ccggcacgcg
     7981 agcgcgcaca gcggcgcctt ctccgagggc ttccacacga ccggattgcc gcacacccac
     8041 gccagcgccg cgttccacgc ccacaccgcc accgggaaat tgaaggcgct tatcaccgcc
     8101 gtcggcccca tcgggtgcca cgtttccatc atgcggtggc ccgggcgttc cgaggcgatc
     8161 gtgaggccgt gcagctggcg cgacaggccc agcgcgaaat cgcagatgtc gatcatctcc
     8221 tggacttcgc cacgggcctc ctcgaggatc tttcccgatt ccagggtgac caggcgcgcg
     8281 agcgactcct tcgcgccgcg aagctcctcg ccgaacagcc gcaccagctc gccgcgcttc
     8341 ggggcgggcc actcgcgcca cgcgaggaag gccgcgtgcg ccgcggcgac gatccgcgcc
     8401 acatcctcct tcgaatccgc gcgcagccgg gccagctcgc cgccatcgac cggggatcgt
     8461 gcgcgcaggc cctcgccggc caccgccgcc aggtcgaggc cgaggtcccg gcaaactccg
     8521 ccgatgtccg tggccgcgtt catgcgtagt acgcgccgaa gcggttcgcc aggaacgcgc
     8581 ccagcggcgc ctgttcctgg cgcacgaacc ccgcctgcgg aagctcgccc tgcaccagca
     8641 ggtccgccat cgcgcagatg cccgcggcgg tggtgacctg gatcgcggaa aagagc
//
LOCUS       DASUEV010000278         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_300045, whole genome shotgun sequence.
ACCESSION   DASUEV010000278 DASUEV010000000
VERSION     DASUEV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300045"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFV55_09005"
     CDS             complement(<1..411)
                     /locus_tag="VFV55_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944480.1"
                     /translation="MATAAVEVRDASKRFGTVRALDAVSLSVEEGEFFALLGPNGAGK
                     TTLISALGGLVRPDSGRLRVMGRDVATESSDARRLLGIVPQEVVFDPFFSVRETLELQ
                     SGYFGLRDNGAWIAELLERLALGPKADANMRSLSG"
     gene            complement(414..716)
                     /locus_tag="VFV55_09010"
     CDS             complement(414..716)
                     /locus_tag="VFV55_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX4944481.1"
                     /translation="MNGEASAEVLALTGALSFESLPGVLAESLAYSSRPDLPDRLTID
                     FSAITVVDSSAVALLLEWRREAARRGKGLFFVNLPANLLALAELYGVSGLIQPCRA"
     gene            complement(713..1363)
                     /locus_tag="VFV55_09015"
     CDS             complement(713..1363)
                     /locus_tag="VFV55_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944482.1"
                     /translation="MTRTLAALAFSAVALLAGAPAIAAPETAPDALVRKSIDEVLALI
                     KADKELQGGNQKKLLALIEEKVLPHFDFSRMTRLAVGRNWAQASDAQKETLTKEFRTL
                     LVRTYSTSLSQYRNQTIDVKPARFAAQDKETVVRTVVNQPGGQPIPIDYGMERVDSGW
                     KVYDVVVDGVSLVTTYRGSFNDQIQKSGIDGLVKTLAERNSSPEAPKAPVKVDVKK"
     gene            complement(1430..1900)
                     /gene="mlaD"
                     /locus_tag="VFV55_09020"
     CDS             complement(1430..1900)
                     /gene="mlaD"
                     /locus_tag="VFV55_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX4944483.1"
                     /translation="MERTTIDLWFGAFVLMGFAALLVLALKVGNLGAERANSTYRVEA
                     RFDNIGSLKVRAPVRSSGVLVGRVADIRFDNERFQATVIIALDARYSFPKDTSASILT
                     SGLLGEQFVGLDAGGDEKKLVEGDRMTITQSAVVLERLIGQFLFSKAGENGAKK"
     gene            complement(1929..2726)
                     /gene="mlaE"
                     /locus_tag="VFV55_09025"
     CDS             complement(1929..2726)
                     /gene="mlaE"
                     /locus_tag="VFV55_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX4944484.1"
                     /translation="MLDRVRMTLTRVGHNFIENVWRVGAMTRFTFVLLAASGTCLRRF
                     RLVMREVYFTGVLSLIIIVVSGLFVGMVLGLQGYETLQTYGAQESVGVLVALSLLREL
                     GPVVAALLFASRAGSAITAEIGLMKATEQLTAMDMMAVSPMARVIAPRFWAGVISMPL
                     LAAIFSAMGVFGGYLVAVVMVGVDNGVFWSQMQAAVDFRYDVMNGVVKSFVFGIAVTV
                     IATFEGYDAPPTAEGVSGATTRTVVSSALAVLALDLVLTALMLRGEY"
     gene            complement(2719..>3406)
                     /locus_tag="VFV55_09030"
     CDS             complement(2719..>3406)
                     /locus_tag="VFV55_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4944485.1"
                     /translation="LLRLIGGQLRPTKGAIRIGGRVVHELDREGLYALRRRMSMQFQF
                     GALFTDMTVFDNVAFPLREHANLTEAMVRDLVLMKLEAVGLRGAHDLMPSELSGGMAR
                     RVALARAVVMDPMLVMYDEPFAGLDPIAMSVVGSLIRRLNDALGATSIIVTHDVVESL
                     QIVDYLYFVAEGRVAGEGTPEQIRAATMPVVRQFVHGEIDGPVPFHYPAPAYDEDVGL
                     AQARADGAHA"
BASE COUNT          590 a         1147 c         1144 g          525 t
ORIGIN      
        1 ccccgagagc gagcgcatgt tggcatcggc cttcgggccc agcgccaggc gctcgagcag
       61 ctccgcgatc cacgcgccgt tgtcgcgcag cccgaagtac cccgactgca actcgagggt
      121 ctcgcggacg ctgaagaagg ggtcgaagac gacctcctgc ggcacgatgc cgaggagcct
      181 gcgcgcatcg ctggattcgg tcgccacgtc ccggcccatg acgcgaagcc gcccggaatc
      241 gggacgcacc aggccgccca gcgcggatat gagcgtcgtc ttgcccgcgc cattggggcc
      301 cagcagggcg aagaactcgc cttcctccac gctgagggac acggcgtcca gcgcgcgaac
      361 cgtcccgaag cgcttggatg cgtcgcgcac ctcgactgca gcggtggcca tgctcaggcc
      421 cggcagggtt ggatgagccc ggagacgccg tagagctcgg ccagcgcaag gaggttggcc
      481 gggaggttca cgaagaaaag gcccttgccg cggcgggcgg cctcgcgtcg ccactcgagg
      541 agcagcgcga cggccgacga gtccaccacg gtgatcgcgg aaaagtcgat ggtgaggcgg
      601 tcgggcaggt ccgggcgcga gctatacgca aggctttcgg cgagcacgcc cggcaggctc
      661 tcgaaggaga gcgcccccgt gagggcaaga acttccgcgc tcgcttcccc gttcacttct
      721 tcacgtcgac cttgacgggc gccttcggcg cttccggaga gctgttgcgc tccgccagcg
      781 tcttcaccag cccgtcgatg cccgatttct ggatctggtc gttgaacgag ccgcgatagg
      841 tggtcaccag cgacacgcca tccaccacca cgtcatagac cttccagccg gaatccacgc
      901 gctccatgcc gtagtcgatg gggatgggct gcccgccggg ctggttgacg accgtgcgca
      961 ccacggtttc cttgtcctgt gcagcgaacc gggccggctt cacgtcgatc gtctggttgc
     1021 ggtactgcga aagcgacgtc gagtaggtcc ggacgagtag cgtgcggaac tccttcgtga
     1081 gggtttcctt ctgggcatcc gaggcctgcg cccagttgcg gcccacggcg aggcgcgtca
     1141 tgcggctgaa atcgaagtgc ggaagcacct tttcctcgat gagggcgagg agcttcttct
     1201 ggttgccgcc ctgcagttcc ttgtcggcct tgatgagggc aagcacctcg tcgatcgact
     1261 tgcgcacgag cgcatcgggc gccgtttccg gcgcagcgat ggctggcgca ccggcaagga
     1321 gcgccacggc ggaaaatgca agtgcggcga gagtgcgtgt catggggggt cctgtctgtt
     1381 ggcgtgactg gcggttggaa cgcgtacggt ggcgggggtt ccccggccgc tacttcttcg
     1441 ccccgttttc accggccttg ctgaagagga actgcccgat cagtcgctcg agcacgacag
     1501 ccgactgggt gatcgtcatg cggtcgccct cgacgagctt cttctcgtcg ccgcccgcat
     1561 cgagccccac gaactgctcg ccgagcaggc ccgaggtcag gatggaggcg ctggtgtcct
     1621 tcggaaacga atagcgcgcg tcgagcgcga tgatgacggt tgcctggaag cgctcgttgt
     1681 cgaagcggat gtcggccacg cggcccacca gcacgccgga actgcgaacc ggagcgcgca
     1741 ccttcaggct gccgatattg tcgaagcgcg cctcgacgcg gtaggtcgaa ttcgcgcgct
     1801 ccgcgcccag gttgcccacc ttgagggcca gcacgagcag cgctgcgaag cccatcagca
     1861 cgaaggcgcc aaaccagaga tcgatggtcg ttctttccat ggtgtccctg cgtatccggg
     1921 tttgcccgtc agtattcgcc gcgcagcatc agagcggtga gcaccaggtc cagggcgagg
     1981 acggcgagcg cggagctcac cactgtgcgg gtcgttgcgc cggacacgcc ctcggcggtc
     2041 ggcggtgcgt cgtacccctc gaatgtcgcg atcaccgtca cggcgatccc gaacacgaaa
     2101 ctcttcacga cgccgttcat cacatcatac cggaagtcca ccgcagcctg catttgcgac
     2161 cagaacacgc cgttgtccac gccgaccatc acgacggcca cgagatagcc gccgaacacg
     2221 cccatcgccg agaagatcgc ggcgaggagc ggcatcgaga tcaccccggc ccagaagcgc
     2281 ggcgcgatca cgcgcgccat gggggatacc gccatcatgt ccatggccgt gagctgctcg
     2341 gtggccttca tgaggccgat ctcggcggtg atcgccgagc cggcacggct cgcgaacagc
     2401 agcgccgcca ccaccgggcc cagctcgcgc agcagcgaca gggcgaccag gaccccgacg
     2461 gattcctgcg ccccgtaggt ctgcagcgtc tcgtagccct ggagcccgag caccatgccc
     2521 acgaagaggc cggagacaac gatgatgatc agcgagagga cgcccgtgaa atagacctcg
     2581 cgcatcacca gccggaagcg ccggaggcag gttcccgagg cggccagcag cacgaaagtg
     2641 aaccgggtca tcgcgcccac gcgccagaca ttctcgatga agttgtgacc cacgcgcgtg
     2701 agggtcatcc gcacccggtc aagcatgggc cccgtccgcg cgcgcctgcg ccagccccac
     2761 gtcctcgtcg taggcgggtg ccgggtagtg gaagggcacg gggccgtcga tctcgccgtg
     2821 cacgaactgg cgcacgaccg gcatcgtggc ggcgcggatc tgctccggag tgccctcgcc
     2881 cgccacgcgg ccttcggcga cgaagtagag gtagtcgacg atctgcagcg attcgacgac
     2941 gtcgtgcgtg acgatgatgg acgtggcgcc gagcgcgtcg ttcaggcggc ggatgaggct
     3001 tcccaccacc gacatcgcga tcgggtcgag gccggcgaac ggctcgtcgt acatcaccag
     3061 catcgggtcc atcaccaccg cgcgcgcgag cgccacgcgc ctggccatgc cgccggaaag
     3121 ctccgagggc atcaggtcgt gcgcgccgcg cagcccgact gcctcgagct tcatgaggac
     3181 caggtcgcga accatggcct cggtgaggtt ggcatgctcg cgcaggggaa acgcgacatt
     3241 gtcgaagacc gtcatgtcgg taaacagggc gccgaactgg aactgcatgc tcatccggcg
     3301 gcgcaacgcg tataggcctt cgcgatcgag ttcgtggacg acccgtcccc cgatgcggat
     3361 cgcgcccttc gtgggccgca actggccgcc gatgagccgc aggagc
//
LOCUS       DASUEV010000279         4625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_757724, whole genome shotgun sequence.
ACCESSION   DASUEV010000279 DASUEV010000000
VERSION     DASUEV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757724"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VFV55_09035"
     CDS             <1..1121
                     /locus_tag="VFV55_09035"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944486.1"
                     /translation="KGVSQRIITAPFSPMGHDTHIASRNATCVALPTNQPIAPLRNKG
                     DLMRITNQEQPFHPIYNYALVTDAEEGLILVGVNTLADGEPRNNFLRRALTWNEGGIL
                     AGARHLTIGGRYVYVAADAGLVVLDLDDPLKPRKVAVVPLKGARASALQFRYLFVTDD
                     EGFKAIDVTVPEKARLVEGGRVALADAGRIYVARTYAYVAAGREGLAIIDVERPREPR
                     LYRRFDDGGKLNDARDVVVATTNASAIAYVADGANGLKVLQLTSPEIQPRFYGFSPEP
                     MPRVIAWRETASPALSLAKGLDRDRAVDETGGQIAVFGRIGARPFTREEQQKFYLRPD
                     GSVWTVSDTGRPGDFLDARAPAGRGPKPPGDAGRAEAR"
     gene            1118..2428
                     /locus_tag="VFV55_09040"
     CDS             1118..2428
                     /locus_tag="VFV55_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX4944487.1"
                     /translation="MNIFPALAIAMAALGARAADAPLPYDARAKHLGVVNCASSLCHG
                     SIGEWKGSNVLQNEYVTWSRVDKHALRASQVLRNDRSKRIAANLGLATPAHEAKICID
                     CHGHNPPSANRGERFKASDGVSCEACHGPAERWIGPHVAPGATHAENLRNGLYPTEDP
                     VALARLCLSCHFGNKDKFVTHRMMGAGHPRISFELDTFTQTQPPHFVVDEDWRRRKGS
                     WDGVRVWAIGQALAAQESLDVVLDPKRGRDGLFPELVVFDCHACHHPMSETRWKPRAG
                     TRPGTIRFNDANLLMLRQIVRQVFPGDSSAFNDLVMRFHKAVAGEGGDAQESALQLHK
                     GLDSVVAKLRARDFGERDLRAILAGLVEDGLAGQFGDYAGAEQATMAIASVLDFLARR
                     GEIADVRAARGALDGVFEAVKDDEKYRRERFVSALALLKTTLAR"
     gene            2434..>4625
                     /locus_tag="VFV55_09045"
     CDS             2434..>4625
                     /locus_tag="VFV55_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4944488.1"
                     /translation="MPTPFDQRTEFRWYKVGIIGSGPAGLSAAAHAAELGASHVLLES
                     ENQASNTIFKYQKGKHVMAEPQILPLRSPMTFAAGKREEILAKWDEDLRRHKVNIRYG
                     QEVTGIAGEDGRFEVTTKRGDTWFCRKIVLAIGLQGNLRKLGVEGDTLEGVQYQLDDP
                     KEYGDETIVVVGAGDAAIENALALSEQNRVILVNRNEEFTRCKEGNLTLVLGAIREGR
                     IEIRYGTSAVRVERTGGEVPLRFEVRSPDGPDAIECHRIIARLGGIPPRRLVEGFGVK
                     FPSADPNAVPELSDTYESNVKGLYIVGALGGYPLIKQAMNQGYEVVESALGRSVEPAD
                     EPLLARKFAKFSRTMSVSSVLDLIQSEVPLLAGINRLQLREFMLESDLVAPRERTVLF
                     ERNDYTNSFYMIVAGEVLVEGDGGKEFRLGAGHFFGELGLISGRRRSNTVRAGPECVL
                     IEASRRAMLKLVASVESVRKEVDETFLKRAVRSYLAPMLPEEELSELVAGGVEVRRYG
                     GGEALFKEGDAPDGLYLLRRGSVMVSRAIAGREVVLSYLSAGNYVGEMALLTDSPRSA
                     TVRAAVTTEAVVLDANAFKQVLGRNPKWREEMEARFLDRLRVNAAMEAAPDPGNIIAF
                     LLQQGMGEATDVLLIDESLCVRCDNCEKACADVHDGTSRLDREAGPTFAEIHVPTSCR
                     HCEHPHCMKDCPPDAIHRAPNGEVFVNDSCIGCGNCEKNCPYGVIQLAP"
BASE COUNT          775 a         1564 c         1628 g          658 t
ORIGIN      
        1 acaagggagt ctcgcagcgc atcatcaccg cgccgttctc gcccatgggg cacgacaccc
       61 acatcgcctc gagaaacgcg acctgcgtgg cgctgcccac caaccagccc atcgcgccgc
      121 ttcgcaacaa gggcgacctg atgcgaatca ccaaccagga acagcccttc cacccgatct
      181 acaactacgc gctggtcacc gatgccgagg agggcctcat cctggtcggt gtgaacacgc
      241 tggccgacgg tgagccacgc aacaacttcc tgcggcgcgc gctcacctgg aacgaggggg
      301 gcatcctcgc tggcgcaagg cacctgacca tcggcggccg ctatgtctac gtggccgcgg
      361 acgcggggct cgtggtcctc gacctcgatg acccgttgaa accgcgcaag gtagcggtgg
      421 tgcccctcaa gggagcgcgc gcctccgcac tgcagttccg ttacctcttc gtcacggacg
      481 acgagggctt caaggcaatc gacgtgacgg tcccggagaa ggcgaggctc gtcgagggcg
      541 gacgcgtggc gctcgccgac gccgggcgca tctacgtggc gcgcacctat gcctacgtcg
      601 ctgctggcag ggaaggtctt gccatcatcg acgtggaaag gccgcgcgag ccgcgcctgt
      661 accggaggtt cgacgacggc gggaagctga acgacgcgcg cgacgtcgtg gtcgcgacga
      721 ccaacgcctc ggcaatcgcc tacgtcgcgg acggggccaa cggcctcaag gtgctgcagc
      781 tcacctcgcc cgagatccag cccaggttct acgggttcag cccggagccg atgccgcgcg
      841 tgattgcctg gcgcgagacc gcctcgcccg cgctctcgct cgccaagggc ctcgaccgcg
      901 accgggccgt cgacgagacc ggcgggcaga tcgcggtctt cggccgcatc ggcgcgcggc
      961 cgttcacgcg cgaggagcag cagaagttct acctgcgtcc ggacggcagc gtctggacgg
     1021 tgagcgacac gggcaggccc ggggacttcc tggacgcgcg cgcgccggcc ggccgtggcc
     1081 cgaagccgcc cggggacgcc ggcagggcgg aggcgcgatg aatatattcc cggcactggc
     1141 gatcgcgatg gcggccctgg gagcgcgcgc cgccgacgcg ccgctgccct acgatgcacg
     1201 cgcgaagcac ctgggcgtcg tcaactgcgc ctccagcctg tgccacggct cgatcggcga
     1261 gtggaagggg tccaacgtcc tgcagaacga gtacgtgacg tggtcccgcg tggacaagca
     1321 cgccctgcgc gcgagccagg tgctgcgcaa cgaccggagc aagcgcatcg cggccaacct
     1381 gggcctcgcg acgccggcgc acgaggcgaa gatctgcatc gactgccatg gccacaatcc
     1441 gccgtccgcc aatcgcggcg agcgcttcaa ggcctccgac ggcgtgagct gcgaggcctg
     1501 ccacggtccc gcggaacgct ggatcgggcc gcacgtggcg ccgggcgcca cgcacgcgga
     1561 aaacctgcgc aacggactct atcccaccga ggaccccgtg gccctcgcgc ggctgtgcct
     1621 gtcgtgccac ttcggcaaca aggacaagtt cgtcacccac cgcatgatgg gggcgggcca
     1681 cccgcgcatc agcttcgagc tggacacctt cacgcagacg cagccgccgc acttcgtcgt
     1741 cgacgaggac tggcgccggc gcaagggcag ctgggacggc gtgcgcgtgt gggccatcgg
     1801 gcaggcgctc gcggcgcagg agagcctcga cgtcgtgctc gacccgaagc gcgggcgcga
     1861 cgggctattc cccgagctgg ttgtcttcga ctgccacgcc tgccaccacc cgatgtcgga
     1921 aacgcgctgg aagccgcgcg cgggaacgcg gcccggcacg atccgcttca acgacgcgaa
     1981 cctcctcatg ctgcgccaga tcgtgcggca ggtgtttccg ggcgattctt ccgcgttcaa
     2041 cgacctggtg atgcgattcc acaaggcggt ggcgggcgaa ggcggggatg cacaggaatc
     2101 cgccctgcaa ttgcacaagg ggctggactc ggttgtcgcg aaattgcgcg cgcgcgattt
     2161 cggcgaacgc gacctgcgcg ccatcctcgc gggtcttgtc gaggacgggc tggccgggca
     2221 gttcggcgac tacgcaggcg cggaacaggc gaccatggcg atcgcgagcg tgctcgattt
     2281 cctcgccagg cgcggggaga tcgcggacgt gcgtgcggcc aggggcgcgc tcgatggcgt
     2341 cttcgaggcc gtgaaggacg acgagaaata ccgccgcgag cgtttcgtgt cggcgctggc
     2401 gctgctcaag acgaccctgg cgaggtgacc ggcatgccga ccccgttcga ccaacgcacg
     2461 gaattccgct ggtacaaggt gggcatcatc ggctcgggcc ccgcggggct ctcggcagcg
     2521 gcccatgccg ccgagctggg cgcctcgcac gtactcctcg agagcgagaa ccaggcctcg
     2581 aacacgatct tcaagtacca gaagggcaag cacgtgatgg ccgagccgca gatcctgccg
     2641 ctcaggagcc cgatgacgtt cgccgccggc aagcgcgagg agatcctcgc gaagtgggac
     2701 gaggacctgc gcaggcacaa ggtcaacatc cgctatgggc aggaagtcac cggcatcgcg
     2761 ggagaggacg gccggttcga ggtgacgacc aagcgcggcg atacctggtt ctgccgcaag
     2821 atcgtgctgg ccatcggact gcagggcaac ctgcgcaagc tcggcgtcga gggcgatacc
     2881 ctggagggcg tgcagtacca gctcgacgac ccgaaggagt acggggacga gaccatcgtc
     2941 gtcgtcggcg cgggcgacgc ggccatcgag aatgcgttgg cgctctcgga gcagaaccgc
     3001 gtcatcctgg tgaaccgcaa cgaggagttc acgcgctgca aggagggcaa cctcacgctc
     3061 gtcctcggcg cgatccggga gggacggatc gagatccgct acgggacgtc ggcggtccgg
     3121 gtggaacgga ccggcggcga ggtgccgttg cgcttcgagg tgaggagtcc cgacgggccc
     3181 gacgccatcg agtgccatcg catcatcgcc cgcctcggcg gcatccctcc gcgcaggctc
     3241 gtggagggct tcggggtgaa attcccctcg gcggacccga acgcggtgcc ggagctctcc
     3301 gacacctacg agtcgaacgt gaagggcctg tacatcgtgg gcgcgctggg cgggtacccg
     3361 ctcatcaagc aggccatgaa ccagggctac gaggtggtcg agtcggccct cgggcgctcg
     3421 gtcgagcccg ccgacgagcc gctcctcgcg cggaaattcg cgaagttttc ccgcacgatg
     3481 tccgtctcga gtgttctgga cctgatccag tccgaggtgc cgctgctcgc cgggatcaac
     3541 cggctgcagt tgcgcgagtt catgctcgaa agcgacctcg tggcgccgcg cgagcgcacg
     3601 gttctcttcg aacgcaacga ctacacgaac tcgttctaca tgatcgtggc gggcgaggtg
     3661 ctcgtcgagg gcgatggcgg caaggaattc aggctggggg ccggccattt cttcggcgag
     3721 ctcggcctca tctcggggcg gcgccggtcg aacacggtgc gcgccggccc cgagtgcgtg
     3781 ctcatcgagg cgtcgcgccg cgcgatgctg aagctcgtgg ccagcgtgga gtcggtgcgc
     3841 aaggaggtcg acgagacctt cctcaagcga gccgtgcgct cctatctcgc gccgatgctg
     3901 ccggaggagg aactctcgga gctggtcgcg ggcggcgtcg aggtgcgcag gtacggcggc
     3961 ggcgaggccc tgttcaagga gggggacgcg cccgacggcc tgtacctgct gcgccgcggc
     4021 tcggtgatgg tctcgcgcgc gatcgcgggg cgggaggtgg tcctgtccta cctctcggcc
     4081 ggcaactacg tgggcgagat ggcgctcctc accgactcgc cgcgatcggc gaccgtccgc
     4141 gcggcggtca ccacggaggc ggtggtgctg gacgccaacg cattcaagca ggtcctcggg
     4201 cgcaatccga agtggcgcga ggagatggaa gcgcgcttcc tcgaccgcct gcgcgtcaat
     4261 gcggcgatgg aggcggcgcc cgacccggga aacatcatcg ccttcctgct gcaacagggc
     4321 atgggagagg cgaccgacgt cctcctcatc gacgagtcgc tgtgcgtgcg ctgcgacaac
     4381 tgcgagaagg cctgcgccga cgtgcacgac ggcacctcgc ggctcgatcg cgaggccggg
     4441 cccacgttcg ccgaaatcca cgtgccgacg tcgtgccggc actgcgagca cccgcactgc
     4501 atgaaggact gcccgccgga cgccatccac cgcgcgccca acggcgaggt attcgtgaac
     4561 gacagctgca tcggctgcgg caactgcgag aagaactgtc cctacggcgt gatccagctg
     4621 gcgcc
//
LOCUS       DASUEV010000280         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1122171, whole genome shotgun sequence.
ACCESSION   DASUEV010000280 DASUEV010000000
VERSION     DASUEV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122171"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..926
                     /gene="hypB"
                     /locus_tag="VFV55_09050"
     CDS             93..926
                     /gene="hypB"
                     /locus_tag="VFV55_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX4944489.1"
                     /translation="MCTTCGCGTDEVRIDGKAHAHGHDHGPAHAHAAGVGAERAVAVE
                     RDILAKNDAEANANRRQFTAKGIFALNLVSSPGSGKTTLLVETIKRLSREIPIAVVEG
                     DQQTSHDADRIRATGSRAVQVNTGKGCHLDAHMVGHAFGHLAPGEDSVLMIENVGNLV
                     CPSAFDLGEAHKVVVLSVTEGEDKPLKYPDMFAAADLMLLNKCDLLPHLDFDVDLCEA
                     NARRINPSIQVLRVSATKGEGLDAWLEWIRGGAREARARRLRGAVPAGCACAGTCATA
                     A"
     gene            942..3257
                     /gene="hypF"
                     /locus_tag="VFV55_09055"
     CDS             942..3257
                     /gene="hypF"
                     /locus_tag="VFV55_09055"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX4944490.1"
                     /translation="MDTIVLAPPIERRSVRVRGTVQGVGFRPFVWRLANELALDGWVL
                     NDAEGVLAEVQGSPRAVDDFLRRLAAEAPPLATVGAIEHETLPVQAVRGFAIVASRGG
                     AATTPVTPDAATCPDCLAELFDPADRRFRYPFINCTHCGPRFTITRGVPYDRPRTTMS
                     SFAMCPACQREYVDPSDRRFHAQPDACPACGPSIALLGEDGARRQEGDVVAAALARIR
                     AGKVVAVKGLGGFHLVCDARNVRAVARLRASKHREEKPFAVMAANVASFLPYARVDEA
                     SRRLLESRERPIVLLEKRPGCDEALAGIANGVSALGAMLPVTPLQWLLFFAASGAAGG
                     TAWIEEPQDLVLVMTSANPGGEPLVLGNDEAVRRLAGIADAFVVHDRDIAVRCDDSLV
                     RATVEGPAFVRRARGYTPRAIRLPRAGPTILATGAWLKNTVCVTRGDEAFLSPHIGDL
                     DNGPTCEALDEAVAHLLAILEVEPAACAHDLHPDFYSTRFAAQFASGHGIPAFAIQHH
                     HAHIAAVCAEHGHSAPAIGLALDGVGLGTDGTAWGGELLRVEGAHFARLGHLGELRLP
                     GGDRAAREPWRVAASVLHTLGRGAEIAQRFPRAGAAIVTEMLERGTNSPVTTSAGRWF
                     DAAAALLGVREVTAFEGQAAMQLEALATGHGPVEPDRSRWRIGADGTLDLLPLAERLA
                     GTQDAAFGAALFHATLIEALAEWVLIAAAREGLATVALGGGCFVNAILSTGLRRQLAS
                     GGLTVLEARQAPANDGGIALGQAWVAMQEFN"
     gene            3259..3489
                     /locus_tag="VFV55_09060"
     CDS             3259..3489
                     /locus_tag="VFV55_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX4944491.1"
                     /translation="MCLAIPAKVVELRDGDQAVIDLAGVRKEISLALVEDVRPGDYVI
                     VHVGYALQKLDPEEAAKTLALFAGVEAANAVA"
     gene            3486..>4298
                     /gene="hypD"
                     /locus_tag="VFV55_09065"
     CDS             3486..>4298
                     /gene="hypD"
                     /locus_tag="VFV55_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX4944492.1"
                     /translation="MKYVDEFRDGGLARTIAARIAAEARPDRTYHFMEFCGGHTHAIS
                     RYGVKDLLPANVVMVHGPGCPVCVLPIGRIDMAIGLALREKVILCTYADTMRVPASGG
                     LSLMKAKAGGADIRMVYSAADALRIAREEPARQVVFFAIGFETTTPPTALVVREAAAL
                     GLANFSVFSNHVLTPSAIAHILESPEVRDLGTVVIDGFVGPAHVSTIIGSQPYEYFAE
                     EYRKPVVIAGFEPLDVMQAIEMLVRQVNEGRAVVENEFSRAVTPDGNAKAKAL"
BASE COUNT          624 a         1509 c         1525 g          640 t
ORIGIN      
        1 tcaggttact tttcagcccg ccttcttgcg acgccaatac gacagaaaag taacctgacc
       61 ccttttccat caccacaacg atggagatga ccatgtgcac cacctgcggt tgcggaacgg
      121 acgaagtgag gatcgacggc aaggcgcatg cgcatggcca tgaccacgga ccggcgcatg
      181 cgcacgcggc cggcgtggga gcggagcgag ccgtggcggt cgagcgcgac atcctggcga
      241 agaacgacgc ggaggccaat gccaaccgcc gccagttcac ggcgaagggc atcttcgcgc
      301 tcaatctcgt ctccagcccc gggtccggca agacgacgct gctcgtcgaa accatcaagc
      361 gcctctcgcg cgaaatcccg atcgcggtgg tcgagggcga ccagcagacc tcccacgacg
      421 cggaccgcat tcgcgccacg ggatcgcggg cggtgcaggt gaacaccggc aagggctgcc
      481 acctcgacgc gcacatggtc ggccacgcgt tcgggcacct ggcgcccggc gaggattcgg
      541 tgctgatgat cgagaacgtg ggcaacctgg tctgcccgtc ggccttcgac ctgggcgagg
      601 cgcacaaggt ggtcgtgctc tcggtgaccg agggcgagga caagcccctc aagtaccccg
      661 acatgttcgc ggccgcggac ctgatgctgc tcaacaagtg cgacctcctt ccgcacctcg
      721 acttcgacgt ggacctgtgc gaggccaacg cgcggcgcat caacccgtcc atccaggtac
      781 tgcgggtatc ggccacgaag ggcgagggcc tggacgcgtg gctggagtgg attcgcggcg
      841 gcgcccgcga ggccagggcg cggcggttgc gcggcgcggt gccggccggt tgcgcctgcg
      901 ccggcacgtg cgcgacggcc gcctgagagt cccgatccgc catggacacc atcgttctcg
      961 ccccgccgat cgagcgccgc agtgtgcgcg ttcgcggcac ggtccagggc gtgggcttcc
     1021 ggcccttcgt ctggcggctg gcgaatgaac tcgcgctcga cggctgggtg ctcaacgacg
     1081 ccgaaggcgt cctggcggaa gtgcagggca gcccgcgcgc ggtggacgat ttcctgcgcc
     1141 ggctcgccgc ggaagcaccg ccgctcgcga cggtaggcgc gatcgagcac gagacgctcc
     1201 ccgtgcaggc ggtacgggga ttcgccatcg tcgcgagccg cggcggcgct gccacgacgc
     1261 cggtgacgcc ggatgcggca acctgtcccg attgcctggc ggaactgttc gaccccgcgg
     1321 accggcgctt ccgctatccg ttcatcaact gcacgcattg cgggccgcgc ttcacgatca
     1381 cgcggggcgt tccgtacgac cgcccgcgca ccacgatgtc ttccttcgcc atgtgcccgg
     1441 cctgccagcg cgagtacgtc gatccgtccg atcgccgctt ccacgcgcag cccgacgcct
     1501 gtccggcctg cgggccgtcg atcgcgctgc tcggagagga tggtgcgagg cgccaggagg
     1561 gcgacgtggt cgccgccgcg ctggcccgca tccgcgccgg caaggtggtc gccgtcaagg
     1621 ggctcggcgg attccacctg gtgtgcgacg cgaggaacgt gcgcgcggtc gcgcggctac
     1681 gcgcgtcgaa gcatcgcgag gaaaagccct tcgccgtcat ggcggccaac gtggcctcct
     1741 tcctgcccta cgcgcgcgtg gacgaggcgt cgcgccggtt gctggaatcg cgcgagcggc
     1801 ccatcgtcct gctggagaag cgccccggct gcgacgaggc cctcgccggg atcgccaacg
     1861 gcgtctcggc cctgggtgcg atgctgcctg tcacgcccct gcagtggctt ctcttcttcg
     1921 cggccagcgg cgccgcgggc ggcacggcgt ggatcgagga gccgcaggac ctcgtgctcg
     1981 tgatgacgag cgccaacccc ggcggcgagc cgctggtcct cggcaatgac gaggccgtgc
     2041 gccggctggc cggcatcgcg gacgccttcg tggtccacga ccgcgacatc gccgtgcgct
     2101 gcgacgacag cctggtgcgc gcgaccgtcg agggccctgc attcgtgcgg cgcgcccgcg
     2161 ggtacacccc gcgcgccatc cgcctgccgc gggcggggcc gacgatcctc gcgaccggcg
     2221 catggctcaa gaacacggtg tgcgtgacgc gcggggacga ggcgttcctg tcgccgcata
     2281 tcggcgatct cgacaacggg cccacctgcg aggcgctgga cgaggcggtt gcgcacctcc
     2341 tcgccatcct cgaggtcgag cccgccgcct gcgcgcacga cctgcacccc gatttctatt
     2401 ccacgcgctt cgcggcacag ttcgcttccg ggcacggcat cccggcgttc gccatccagc
     2461 accaccacgc ccatatcgcg gccgtttgcg ccgagcacgg acacagcgca ccggcgatcg
     2521 gcctcgcgct cgacggcgtg gggctcggca cggacggcac ggcatggggc ggcgagctcc
     2581 tgcgcgtgga aggcgcgcat ttcgcgcgac tgggacacct gggtgaactg cgcctgcccg
     2641 gtggcgatcg cgccgcgcgg gagccctggc gggtcgccgc atccgtcctg cacaccctgg
     2701 gccgtggtgc cgagatcgca cagcgcttcc cgagggccgg cgcggcgatc gttaccgaga
     2761 tgctggagcg tggcacgaat tcgccggtga ccacttccgc gggccgctgg ttcgacgcgg
     2821 cggccgcgct gctgggcgtg cgcgaggtga cggccttcga agggcaggcc gcgatgcagc
     2881 tcgaggcgct ggccacgggg cacggtcccg tggagcccga ccgctcgcgg tggcgcatcg
     2941 gcgccgacgg cacgctggat cttcttcccc tcgccgagcg cctcgcgggc acgcaggatg
     3001 cggctttcgg cgccgcgctt ttccatgcca cgctcatcga ggcgctggcg gagtgggtat
     3061 tgatcgccgc cgcgcgcgaa gggcttgcca cggtggcgct gggcggcggc tgtttcgtga
     3121 atgccattct ttcgacgggg ctgcggcgcc aactcgcatc cggcgggctc acggtgctcg
     3181 aggcccgcca ggccccggcc aacgacggcg gcatcgcgct ggggcaggcg tgggtcgcca
     3241 tgcaggagtt caactgacat gtgcctggcc attcccgcga aagtggtgga gttgcgtgac
     3301 ggcgaccagg cggtcatcga cctggcgggc gtgcgcaagg aaatctcgct ggccctcgtc
     3361 gaggacgtgc ggcccggcga ctacgtgatc gtccacgtgg gctacgcgct gcagaagctc
     3421 gaccccgagg aggccgcgaa gacgctcgcc ctcttcgccg gggtcgaagc ggccaacgcc
     3481 gtcgcatgaa atacgtcgac gaattccgcg acggcggcct ggcgagaacc atcgccgcgc
     3541 gcatcgcggc cgaggcgcgg ccggatcgca cctaccattt catggaattc tgcggcgggc
     3601 acacgcacgc gatctcgcgc tacggcgtga aggacctcct tccggcgaat gtcgtgatgg
     3661 tgcacggccc cggctgcccg gtgtgcgtgc tgcccatcgg tcgcatcgac atggccatcg
     3721 ggctcgccct gcgcgagaag gtgatcctgt gcacctatgc cgacacgatg cgcgtgccgg
     3781 cctcgggcgg cctgtcgctc atgaaggcca aggcgggggg cgccgacatc cgcatggtct
     3841 attcggcggc ggacgcgctg cgcatcgcgc gcgaggaacc ggcgcgccag gtcgtcttct
     3901 tcgccatcgg cttcgagacg acgacgccgc ccaccgcgct ggtcgtgcgc gaggccgccg
     3961 ccctggggct cgcgaacttc agcgtgttca gcaatcacgt gctgacgccc tcggccatcg
     4021 cgcacatcct cgagtccccg gaggtgcgcg acctggggac ggtcgtgatc gacggcttcg
     4081 tgggcccggc gcacgtctcg accatcatcg gcagccagcc ctacgagtac ttcgcggagg
     4141 aataccgcaa gcccgtggtc atcgccggct tcgagccgct ggacgtcatg caggccatcg
     4201 agatgctcgt gcggcaggtg aacgagggac gcgcggtggt ggagaacgag ttctcgcgcg
     4261 ccgtgacacc ggatggcaat gcaaaggcaa aggcgctg
//
LOCUS       DASUEV010000281         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_518302, whole genome shotgun sequence.
ACCESSION   DASUEV010000281 DASUEV010000000
VERSION     DASUEV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518302"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFV55_09070"
     CDS             complement(<1..822)
                     /locus_tag="VFV55_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX4944493.1"
                     /translation="MTLLEVTLLAPLAGAVLLAVIGHRPLGGWVNVATGAITFGVSFA
                     MAIDVFANGPRLTPNRMFYIDAFNVYLVALTAFVGMTAAIFSRPYMRHEVERGRVTDR
                     RLRLYHAMYQSFIFSMLLALSTNNLGVLWVAMEAATLTTVLLVSLYRTPEAIEAAWKY
                     FILCGVGIAQALFGTVLVYFAAENKLGRDRDDTMLWTVLHDVAPGTLDPAVISLAFVF
                     LLVGYGTKVGLVPLHNWLPDAHSEGPTPMSAVLSGLLLNVALYALVRVKMIVDASL"
     gene            complement(819..1544)
                     /locus_tag="VFV55_09075"
     CDS             complement(819..1544)
                     /locus_tag="VFV55_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEX4944494.1"
                     /translation="MELSELGLFSQAIVLFAALVLFTSFIMLTQVRMLPLIFTFAWQG
                     VLLAIVTALVAFVSRHPHLYVSAALTVALKVVLIPWLLYRLSIRLHLQYDAETLEHPS
                     LILLAGAGLVIFSYFVALPIVHLSGLATRNTIAVSIAVELVGMLLMCVRRTAVAQVIG
                     FMSMENGLFFSAVVSTYGMPLIVELGVAFDILVAAILFGVFFIHLRDSFDSLDVNRLN
                     RLSESGDAPPAEAAAGRAAGGHA"
     gene            complement(1545..2507)
                     /locus_tag="VFV55_09080"
     CDS             complement(1545..2507)
                     /locus_tag="VFV55_09080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX4944495.1"
                     /translation="MDRELIAWPLAVAQAALFAASAPLLAGWVKRVKCHLQNRAAPSV
                     WQPYRDLAKLLLRKDVVLAQNASRLFRAVPYVVFGATLLAAGVVPLIAVKLPTAAIAD
                     VIVLVAFFALARFFTALAGLDIGTAFGGMGASREMMVAALAEPAMLMAVFTLSMTATT
                     TNLSVATEFVLNAGLVLRPSFLFALLALGMVAVAESSRIPVDNPATHLELTMLHEAMI
                     LEYSGRYLGVIEWAAQLKLMIYAVVIIDFFFPWGIATDFSARALAVGAIAVTAKLMLL
                     AVILVAWETVLAKMRLFRVPQFLGFAFLLSVLGMLTHVVLEAGG"
     gene            complement(2492..>3031)
                     /locus_tag="VFV55_09085"
     CDS             complement(2492..>3031)
                     /locus_tag="VFV55_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944496.1"
                     /translation="SMIEAMAPVTRLLVNESLPSATAEGWLWLTPISAQVASYSAPFV
                     VAGIAVVFGMGYFFLKRGAAPARMSSPWDCGFGQLNHRMQYTSTAFSQPIRRVFGATW
                     KVEEQIDRVEDEGPIPRLRGIHHQVHVHDWSWLVCYVPIGKFVLAAARRIGFIQTGNI
                     HTYLKYSFATLLVFLWIVS"
BASE COUNT          599 a          979 c         1075 g          378 t
ORIGIN      
        1 cagggacgcg tcgacgatca tcttcacgcg caccagcgcg tagagcgcga cgttgagcag
       61 cagcccggag agcaccgccg acatcggcgt gggcccctcg gagtgggcgt cgggcagcca
      121 gttgtgcagc ggcacgaggc ccaccttggt gccgtagccc acgagcagga acacgaaggc
      181 gagcgaaatg accgccggat ccagggtgcc cggcgcgacg tcgtgcagca cggtccagag
      241 catcgtgtcg tcgcgatccc gcccgagctt gttctcggcg gcgaagtaga cgagcacggt
      301 gccgaacagg gcctgcgcga tcccgacccc gcagaggatg aagtacttcc acgccgcctc
      361 gatcgcctcc ggcgtgcggt acagggagac gagcagcacg gtggtgagcg tggccgcctc
      421 catcgccacc cagaggacgc cgaggttgtt ggtcgaaagc gccagcagca tcgagaagat
      481 gaagctctgg tacatcgcgt ggtaaaggcg caggcgccgg tcggtgacgc gcccgcgctc
      541 gacctcgtgg cgcatgtagg ggcgggagaa gatggccgcg gtcatgccga cgaaggccgt
      601 cagggcgacg aggtagacgt tgaaggcgtc gatgtagaac atgcggttcg gggtaagccg
      661 cggcccgttc gcgaagacgt cgatcgccat ggcgaacgag acgccgaagg tgatcgcgcc
      721 ggtcgccacg ttgacccacc cgccgagggg tcggtggccg atcacggcaa gaagcacggc
      781 cccggccagc ggcgcgagaa gggtgacctc gagcagcgtc atgcatgccc tccggcagcg
      841 cggccggccg ccgcctcggc aggcggcgca tcgccggatt cggacaggcg gttcagccgg
      901 ttcacgtcga gggagtcgaa ggaatcgcgc aggtggatga agaacacgcc gaacaggatc
      961 gcggccacga ggatgtcgaa ggcgacgccc agctcgacga tgagcggcat gccgtaggtc
     1021 gagacgaccg ccgagaagaa gagcccgttc tccatcgaca tgaacccgat cacctgcgcc
     1081 acggcggtcc ggcgcacgca catgagcagc atccccacga gctcgaccgc gatgctgacg
     1141 gcgatcgtgt tccgggtggc gagccccgaa aggtgcacga tgggaagcgc cacgaagtag
     1201 ctgaagatca cgagtcccgc gccggcaagg aggatcagcg aagggtgctc cagcgtttcc
     1261 gcgtcgtact gcaggtgcag ccggatgctc aggcggtaga gaagccacgg gatcagcacc
     1321 accttgagcg cgaccgtgag cgccgccgag acgtagaggt gggggtgcct ggagacgaag
     1381 gcgaccagcg ccgtgacgat cgcgagcagc acgccctgcc acgcgaaggt gaagatgagc
     1441 ggcagcatcc gcacctgggt gagcatgatg aaggacgtga acagcaccag cgcggcgaac
     1501 aggacgatgg cctggctgaa cagccccagc tcggagagtt ccatctagcc tcccgcctcc
     1561 agcaccacgt gcgtgagcat gcccagcacg gacaggagga aggcgaagcc caggaactgc
     1621 ggcacgcgga acagcctcat cttggcgagc acggtctccc aggccaccag aatcacggcg
     1681 agaagcatga gcttcgcggt caccgcgatg gcgcccaccg ccagcgcccg ggcgctgaaa
     1741 tccgtcgcga ttccccaggg gaagaagaag tcgatgatga ccaccgcgta gatcatcagc
     1801 ttgagctgcg ccgcccactc gatcaccccc aggtagcgcc cgctgtactc caggatcatg
     1861 gcctcgtgca gcatcgtgag ttccaggtgg gtggccggat tgtccaccgg gatgcggctg
     1921 gactccgcga ccgccaccat ccccagcgcg agaagggcga aaaggaagga gggccgcagc
     1981 accaggcccg cgttcagcac gaactccgtc gcgaccgaca ggttcgtcgt ggtcgcggtc
     2041 atggacagcg tgaataccgc catgagcatg gccggctcgg ccagtgcggc caccatcatc
     2101 tcgcgcgagg cgcccatgcc gccgaaggcg gtgccgatgt cgagcccggc cagcgcggtg
     2161 aagaaccgcg cgagcgcgaa gaacgccacc aggacgatca cgtccgcgat cgcggccgtg
     2221 ggcagcttca cggcgatgag cgggaccact ccggcggcga gcagggtcgc gccgaacacg
     2281 acgtagggga cggcccggaa cagccgcgag gcgttctgcg cgagcaccac gtccttgcgc
     2341 agcagcagct tggccaggtc gcggtacggc tgccacaccg atggcgcggc gcggttctgc
     2401 aggtggcact tgacgcgctt cacccagccg gcgagcaacg gagccgacgc ggcgaagagc
     2461 gccgcctgcg ccacggcgag gggccaggcg atcagctcac gatccacagg aacaccagca
     2521 gggtggcgaa ggaatacttc aggtaggtgt ggatgttccc ggtctggatg aagccgatgc
     2581 gccgcgcggc cgcgagcacg aacttcccga tcgggacata gcacacgagc cacgaccagt
     2641 cgtggacgtg cacctggtgg tggatgccgc gcaggcgcgg gatcggtccc tcgtcctcga
     2701 cgcggtcgat ctgctcctcg accttccacg tcgcgccgaa gacgcgccgg atcggctggc
     2761 tgaacgcggt ggaggtgtac tgcatgcggt ggttgagctg cccgaagccg cagtcccacg
     2821 gggaggacat ccgggcgggc gcggcaccgc gcttcaggaa gaaatagccc atcccgaaca
     2881 cgacggcgat gccggcgacg acgaagggcg cggagtagga agccacctgc gccgagatcg
     2941 gcgtgagcca cagccagccc tcggcggtgg ccgacgggag cgattcgttc accagcaggc
     3001 gggtgaccgg cgccatcgcc tcgatcatcg a
//
LOCUS       DASUEV010000282         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_645739, whole genome shotgun sequence.
ACCESSION   DASUEV010000282 DASUEV010000000
VERSION     DASUEV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645739"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VFV55_09090"
     CDS             complement(<1..150)
                     /locus_tag="VFV55_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944497.1"
                     /translation="MNPPAAIETRSTCCYCGTGCGVVIRTEGARIVAVAGDERHPSSR
                     GRLCTK"
     gene            complement(147..512)
                     /gene="nirD"
                     /locus_tag="VFV55_09095"
     CDS             complement(147..512)
                     /gene="nirD"
                     /locus_tag="VFV55_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEX4944498.1"
                     /translation="MAYIPIGRLEDIAPLGARRYRRADGVEIAVFRTAADTVFALRDK
                     CPHKGGPLSQGIVFGESVACPLHNWCIGLADGTAAAPDEGVVERFAVKLQDGQVLLDP
                     DSGIVEKRSCSPVAVVAAP"
     gene            complement(514..>2184)
                     /gene="nirB"
                     /locus_tag="VFV55_09100"
     CDS             complement(514..>2184)
                     /gene="nirB"
                     /locus_tag="VFV55_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEX4944499.1"
                     /translation="GLHCQRGIVVNDTLQTFDPRIYAVGECANHRGTAYGLVAPLFEM
                     AKVCANHLAEYGIARYTGSVTSTKLKVTGVDLFSAGDFTGGEGTEDIVLADPTGGVYK
                     KLVIKDDKLAGAVLYGDTVDGTWYFRLLREGSSVSPIRDRLMFGESHLGDTGHQGQNR
                     ALALPDTAEVCGCNGVCKGTIVKAIKEKGLFTLEEVRTHTKASGSCGSCTGLVEQILM
                     FTAGGDYSDAPKKKAVCACTDRTHQEVRSAIREHHLLDIPSAFRFLEWRTPNGCASCR
                     PAMNYYLLSTWPHEAKDDPQSRYINERSHANIQKDGTYSVIPRMWGGETSAAELRRIA
                     HVVDKYGIPTVKVTGGQRLDLLGVRKEDLPAVWKDLDMPSGHAYAKALRTVKTCVGSE
                     WCRFGTQDSTRMGQELERSLWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWE
                     IYVAGNGGIKTEVAQFLCKVKTREEVLEYAGAFLQLYREEGFYLERTVHFVARVGLDH
                     VKKRVLEDAGGRKALWERLQFALDGEPDPWHEPDKARVDLRQFETIGS"
BASE COUNT          322 a          744 c          750 g          368 t
ORIGIN      
        1 cttggtgcag aggcgcccgc ggctcgacgg gtgccgctcg tcgcccgcga ccgcgacgat
       61 gcgcgcgccc tcggtgcgga tgacgacgcc gcagccggtg ccgcaatagc agcaggtgga
      121 gcgcgtctcg atcgcggcgg gagggttcac ggtgccgcga ccacggccac gggcgagcag
      181 gagcgcttct cgacgatgcc ggaatccgga tcgaggagca cctggccatc ctgcagcttc
      241 acggcgaatc gttccaccac gccttcgtcg ggtgcggcgg ccgtgccgtc ggccagtccg
      301 atgcaccagt tgtgcaacgg gcacgccacc gactcgccga acacgatccc ctgcgaaagc
      361 gggccgccct tgtgcgggca tttgtcgcgc agcgcgaaca ccgtgtcggc cgcggtgcgg
      421 aagaccgcga tctccacgcc gtccgcgcgc cggtagcgcc gcgcgccgag cggagcaatg
      481 tcctcgaggc ggccgatggg aatgtaggcc atgtcagctc ccgatcgtct cgaactggcg
      541 caggtccacg cgcgccttgt ccggctcgtg ccagggatcg ggctcgccgt cgagcgcgaa
      601 ttgcagccgc tcccacagcg ccttgcgccc cccggcatcc tccagcacgc gcttcttcac
      661 gtggtcgagg ccgacgcgcg ccacgaaatg cacggtgcgc tcgaggtaga agccctcctc
      721 gcggtagagc tgcaggaacg cgccggcgta ttccagcact tcctcgcgcg tcttcacctt
      781 gcacaggaac tgcgccacct cggtcttgat gccgccgttg cccgcgacat agatctccca
      841 gccggagtcg acgccgatca cgcccacgtc cttgatcccg gactcggcgc agttgcgcgg
      901 gcagccggag acggcgagct tcaccttgtg cggcgcgtac atccgccaca gcgagcgctc
      961 gagttcctgg cccattcgcg tggaatcctg cgtgccgaag cggcaccatt cgctgcccac
     1021 gcaggtcttc acggtgcgca gcgccttggc gtaggcgtgg ccggagggca tgtcgaggtc
     1081 cttccatacc gcgggcaggt cctccttccg cacgcccagc aggtcgaggc gctggccgcc
     1141 ggtcaccttc accgtcggga tcccgtactt gtccacgacg tgggcgatgc gccgcagttc
     1201 ggcagccgaa gtctcgccgc cccacatgcg cgggatcacc gagtaggtgc cgtccttctg
     1261 gatgttggcg tgcgagcgct cgttgatgta gcgcgattgc gggtcgtcct tcgcctcgtg
     1321 cggccaggtc gagagcaggt agtaattcat cgcgggccgg caggaggcgc atccgttggg
     1381 ggtgcgccat tcgaggaagc ggaaggcgga cgggatgtcg aggagatgat gctcgcggat
     1441 ggcgctgcgc acctcctggt gcgtgcggtc ggtgcaggcg cacacggcct tcttcttcgg
     1501 cgcgtcggag taatccccgc cggcggtgaa catcaggatc tgttcgacga ggccggtgca
     1561 cgagccgcag gagccggacg ccttggtgtg cgtgcgcact tcctccaggg tgaacaagcc
     1621 cttctccttg atcgccttca cgatggtgcc cttgcacacg ccgttgcacc cgcagacctc
     1681 ggccgtgtcg ggaagggcga gggcgcggtt ctggccctgg tggccggtgt cgcccaggtg
     1741 cgattcgccg aacatgaggc ggtcgcggat gggcgagacg ctggagcctt cgcgcagcag
     1801 cctgaagtac caggtgccgt ccaccgtatc gccgtagagc acggcgccgg cgagcttgtc
     1861 gtccttgatg acgagcttct tgtacacgcc gcccgtgggg tcggcgagca cgatgtcctc
     1921 cgtgccctcg ccacccgtga aatcgccggc cgagaagagg tcgacgcccg tcaccttgag
     1981 cttcgtcgag gtgacagatc ccgtgtagcg cgcgatgccg tactccgcaa ggtggttggc
     2041 gcacaccttg gccatctcga agagcggggc gacgagcccg taggccgttc cgcgatggtt
     2101 ggcgcattcg cccacggcgt agatgcgcgg gtcgaacgtc tgcaacgtgt cgttcaccac
     2161 gatgccgcgc tggcagtgca ggcc
//
LOCUS       DASUEV010000283         4404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_518770, whole genome shotgun sequence.
ACCESSION   DASUEV010000283 DASUEV010000000
VERSION     DASUEV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518770"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..985)
                     /locus_tag="VFV55_09105"
     CDS             complement(74..985)
                     /locus_tag="VFV55_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944500.1"
                     /translation="MDALRRFLDGDVFYSFKRSPLVIVAAVATLVCIASAVFAGWVAP
                     HNPFDLKTLNLLDAFTPPAWTEKGNPDYLLGTDDQGRDVLSAIMYGSRVSLMVGFLAV
                     VLAMTIGIALGLISGYVGGKVDAFIMRIADVQLSFPAILIALLIDGVVRVALPKESHD
                     TLALYVLVLAIAASGWVQYARTVRGSTLVERNKEYVQAARVIGRHPVAIMFSHVLPNV
                     LGPVLVLATLHIGTAIITEATLSFLGVGVPPTQPSLGTLIRIGNDFLFSGEWWITVFP
                     GAWLVIMVLAMNLLGDWLRDALNPKLR"
     gene            complement(985..1971)
                     /locus_tag="VFV55_09110"
     CDS             complement(985..1971)
                     /locus_tag="VFV55_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944501.1"
                     /translation="MIAFVIRRLLQGVIVMLVVGFIAFSLFNFVGDPVSLMLPPEATQ
                     ADRQAMRESLGLDRPFYRQFATFVANAVQGNFGISLRLGRPVSTLLSERLPATLELAF
                     VAASMALLVGVPMGVYTGLKRTAWLSRVFLTLSLVGVSLPTFLIGIFLILIFAVWLGW
                     LPSFGRGDTVMVGPWSTGFLTVSGLRALILPSITLGLFQLTLIMRLVRSEMLEVLRTD
                     YIKFARARGLTNRSIHFGHALKNTLVPVITITGLQLGAIIAFAIITETVFQWPGMGLL
                     FIQAVQFADIPVMAAYLCLIGLIFVVINLVVDMLYYFVDPRLRIDRATVGGH"
     gene            complement(1971..3548)
                     /locus_tag="VFV55_09115"
     CDS             complement(1971..3548)
                     /locus_tag="VFV55_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944502.1"
                     /translation="MPLGRTIALLALACLPLAAGARTLRYASQDDPQTLDPHSANLLS
                     TSRLTSNVYEPLVWRDKDWKVIPWLATSWTQPDAKTWRFKLRAGVKFHDGSAFTADDV
                     VFSVERALSPNSQLKVALQGVEKAVKVDALTVDLVMREPNPVLLSHMTNFRIMSKAWS
                     VKNGAVRPQDYTAKEDTYAARNANGTGAFMVKERQTDVKTVLVAHKDWWGHAAGRNEG
                     NLTEVVMLPIKSNATRLAALLSGEVDLVIDPPTQDVARLRADPGVKIVEGGELRVQYI
                     AFDVFRDDLLYGKANGKNPFKDIRVRQAVAHAIDADAIRAKVMRGFSRPAGAILTPGV
                     QGYSADADRRLPYDRDKARKLLAEAGYPNGFDVTLDAGNIQPAADIAQAVAAMLSQVG
                     INVRPNIVPQSNYFPKIEKYDTSFYVLSWGAGVTGDALYTLQALLHSVSRKGEGDFNM
                     GRWSNARMDELIQQLQVESQPAKRDAIVREALLLAGRELPIVTIHQPLIPWAMRRNVS
                     AWFSPVNTVYFYRVRVD"
     gene            complement(3617..>4404)
                     /locus_tag="VFV55_09120"
     CDS             complement(3617..>4404)
                     /locus_tag="VFV55_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944503.1"
                     /translation="EGNENRTVFLGMDQWRDELAYSSVKGKNPFKDKRVREAFQTAID
                     VNALKTQVMRGLSIPTAVMFAPQVSGYQKDLDKVKPADAARAKKLLAEAGYPSGFEVT
                     LDCPNNRYIADEKLCVAIAGMLARIDVKVRVNAMPRAQYFPKIQNFDTSFYMLGWGVP
                     TFDSQYAVQSLLRSHVDKSADGDYNLGKYSNAKVDAAVDKLKTEIDPAKRAALTREIL
                     QVHMADVGHIPLHHQVIPWAMRSNVSVVHRADNRLTVKWVRIN"
BASE COUNT          747 a         1380 c         1576 g          701 t
ORIGIN      
        1 gcgaaggcgc cggcggcgag caacgccggg acgagaagcg gggagaggat cgagcgtttc
       61 atgggaatct ccactagcgc agcttcgggt tgagggcgtc gcgcagccag tcgcccagca
      121 ggttcatcgc cagcaccatg atcaccagcc aggcccccgg gaagacggtg atccaccatt
      181 ccccggagaa caggaagtcg ttgccgatgc ggatcagggt gccgagcgag ggctgggtgg
      241 gaggcacgcc gacgcccagg aaggacagcg tcgcctccgt gatgatggcg gtgccgatgt
      301 gcaacgtggc gagcaccagg acgggcccga gcacgttggg cagcacgtgc gagaacatga
      361 tcgcgaccgg gtgccgcccg atcacgcgcg ccgcctgcac gtattccttg ttgcgttcca
      421 cgagcgtgga gccgcgcacc gtccgcgcgt actgcaccca gcccgatgcg gcgatggcga
      481 gcaccagcac gtagagcgcg agcgtgtcgt gggactcctt gggcagggcc acgcgcacca
      541 cgccgtcgat gaggagcgcg atgaggatcg cggggaagga gagctgcacg tccgcgatgc
      601 gcatgatgaa ggcgtcgacc ttgccgccca cgtagccgga aatgaggccc agcgcgatgc
      661 cgatcgtcat cgccagcacc accgccagga atccgaccat gagcgacacg cgcgagccgt
      721 acatgatggc cgagaggacg tcgcggccct ggtcgtcggt gccgagcagg taatccggat
      781 tgcccttctc cgtccaggcc ggcggcgtga acgcgtcgag caggttgagc gtcttcaggt
      841 cgaaggggtt gtgcggcgcc acccagcccg cgaacacggc tgatgcgatg cataccagcg
      901 tggccacggc ggccacgatg acgagcggcg agcgcttgaa gctgtagaag acgtcgccat
      961 ccaggaaccg ccgcagggcg tccatcaatg tccccccacc gtggcgcggt cgatgcgcaa
     1021 ccgcgggtcg acgaagtaat agagcatgtc caccacgagg ttgatcacga cgaagatcag
     1081 gccgatgagg cagaggtacg ccgccatcac cgggatgtcg gcgaactgca cggcctggat
     1141 gaagaggagt cccatcccgg gccactggaa cacggtctcg gtgatgatgg cgaaggcgat
     1201 gatggcgccc agctgcaggc cggtgatcgt gatcaccgga accagcgtgt tcttgagcgc
     1261 gtggccgaag tggatggaac ggttggtgag tccccgggcg cgcgcgaact tgatgtagtc
     1321 cgtgcgcagc acctcgagca tctcgctgcg cacgagacgc atgatgagcg tgagctggaa
     1381 gaggcccagg gtgatggagg gcaggatcag cgcgcgcagg ccggagacgg tgaggaaccc
     1441 cgtggaccag gggcccacca tcaccgtgtc cccgcgcccg aaggagggca gccagcccag
     1501 ccacacggcg aagatgagga tgaggaagat gccgatgagg aaggtgggaa gcgacacgcc
     1561 gacgagggaa agggtgagga agacgcgcga gagccacgcc gtgcgcttca ggcccgtgta
     1621 gacgcccatc ggaacgccta cgaggagcgc catcgaggcc gccacgaagg cgagctccag
     1681 cgtggccggc aggcgttcgg acaggagtgt cgacacgggg cgcccgagcc gcagcgagat
     1741 gccgaaattg ccctgcacgg cgttggccac gaaggtcgcg aattgcctgt agaacggccg
     1801 gtcgagcccg agggattcgc gcatcgcctg gcggtcggcc tgcgtggctt cgggcggcag
     1861 catgagcgac acggggtcgc ccacgaaatt gaagagcgaa aaggcgatga agcccaccac
     1921 gagcatcacg atgacgccct ggagcagccg gcggatgacg aaggcgatca tcagtcgacc
     1981 cgcacccggt agaaatacac cgtgttcacc ggcgagaacc aggccgacac gttcctgcgc
     2041 atggcccagg ggatcagcgg ctggtggatg gtgacgatcg gcagttccct gcccgcaagg
     2101 aggagggctt cgcgcacgat ggcgtcgcgc ttcgccggct ggctctcgac ctgaagctgc
     2161 tggatcagct cgtccatgcg cgcgttcgac cagcggccca tgttgaagtc cccctcgccc
     2221 ttcctgctga cggagtgcag gagcgcctgc agcgtgtaga gcgcgtcgcc ggtgacgccc
     2281 gcgccccacg agagcacgta gaagctggtg tcgtacttct cgatcttcgg gaagtagttg
     2341 gactgcggga cgatgtttgg gcgcacgttg atgcccacct gcgagagcat cgccgccacc
     2401 gcctgcgcga tgtcggcggc cggctggatg ttgcccgcgt ccagcgtcac gtcgaatccg
     2461 ttggggtagc ccgcttccgc cagcagcttc ctcgccttgt cgcggtcgta cggcaggcgc
     2521 cggtcggcgt cggccgagta gccctgcacg cccggcgtga ggatggctcc ggccggccgc
     2581 gagaagccgc gcatcacctt ggcgcggatc gcgtcggcat cgatggcgtg ggcaacggcc
     2641 tggcgcacgc ggatgtcctt gaacgggttc ttcccgttcg cctttccgta gaggaggtcg
     2701 tcgcggaaga cgtcgaacgc gatgtactgc acgcgcagct ccccgccctc gacgatcttc
     2761 acgcccgggt cggcgcgcag ccttgccacg tcctgcgtgg ggggatcgat caccaggtcc
     2821 acttcgcccg agagcagggc ggcgagccgc gtggcgttcg acttgatggg cagcatcacc
     2881 acctcggtga ggttgccttc gttcctgccc gcggcgtggc cccaccagtc cttgtgcgcg
     2941 acgagcaccg tcttcacgtc ggtctggcgc tccttcacca tgaaggcgcc ggtgccgttg
     3001 gcgttgcggg cggcataggt gtcctccttg gcggtgtaat cctgcgggcg cacggcaccg
     3061 ttcttcaccg accaggcctt gctcatgatg cggaagttgg tcatgtgcga gagcagcacc
     3121 gggttgggct cgcgcatcac caggtccacg gtgagggcgt ccaccttcac cgccttttcc
     3181 acgccctgca gcgcaacctt gagctgcgag ttgggggaga gcgcgcgctc caccgagaac
     3241 acgacgtcgt cggcggtgaa ggccgacccg tcatggaact tcacgccggc gcgcagcttg
     3301 aagcgccagg tcttcgcatc gggctgcgtc caggaggtcg caagccacgg gatgaccttc
     3361 cagtccttgt cgcgccagac cagcggttcg tagacgttgg acgtcagccg cgaggtggac
     3421 aggaggttgg cggagtgcgg gtcgagggtc tggggatcgt cctggctcgc gtagcgcagc
     3481 gtcctggccc cggcggcgag cggcaggcag gcgagcgcga ggagggcgat ggtgcgtccg
     3541 aggggcatgg gcgggagcat aacgcagccg gccgcgcacg acgcggggcc ggagggaacc
     3601 gcggcggatc aggcgctcag ttgatcctga cccacttgac ggtgaggcgg ttgtccgcgc
     3661 ggtgcacgac gctcacgttc gagcgcatgg cccacgggat cacctggtgg tgcagcggga
     3721 tgtggcccac gtcggccatg tggacctgca ggatttcgcg ggtgagcgcg gcgcgcttgg
     3781 ccgggtcgat ctcggtcttc agcttgtcca cggcggcgtc gaccttcgcg ttggagtact
     3841 tgccgaggtt gtagtcgccg tccgcgctct tgtcgacgtg gctgcgcagc aggctctgca
     3901 cggcgtattg cgagtcgaag gtgggcacgc cccagcccag catgtagaag ctggtgtcga
     3961 agttctggat cttggggaag tactgcgcgc gcggcatcgc gttcaccctc accttcacgt
     4021 cgatgcgggc gagcatgccg gcgatggcca cgcacagctt ctcgtcggcg atgtagcggt
     4081 tgttcgggca gtcgagcgtc acctcgaagc cgctggggta gccggcctcg gcgaggagct
     4141 tcttcgcgcg cgccgcgtcg gcgggcttca ccttgtccag gtccttctgg tagccgctca
     4201 cctgcggggc gaacatgacc gccgtcggga tcgaaaggcc gcgcatcacc tgggtcttga
     4261 gggcgttcac gtcgatggcc gtctggaagg cctcgcgcac gcgcttgtcc ttgaacgggt
     4321 tcttgccctt cacgctcgag taggcgagtt cgtcgcgcca ctggtccatg ccgaggaata
     4381 cggtgcggtt ctcgttgcct tcca
//
LOCUS       DASUEV010000284         6163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_176977, whole genome shotgun sequence.
ACCESSION   DASUEV010000284 DASUEV010000000
VERSION     DASUEV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176977"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFV55_09125"
     CDS             <1..993
                     /locus_tag="VFV55_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX4944504.1"
                     /translation="IPFTQVKLVWWGLFHGMADGLKMLLKEDFKPRTYDWYAYAVAPW
                     VVFTPVLLVFAVIPFGGTLSPAQLWAPLAQWFGDKSYPMQIAKLDAGLLMVFAFSGLT
                     VIGAMLAGWSSSNKFSLLGALRAGSQMISYELVLGLTVLGLILIYGTVDLDVMVRHQS
                     GTVLGFLPAWGIVYQPFAATLFLAAAIAENKRIPFDLPEAESELIAGYYTEYSAMKMG
                     LFMFAEFIEIAIVGALFTTLFLGGYNLPYMSDAGFAFPGGGSVALPHAAVVFTQLAVF
                     LLKVFVVCSFQILIRWTLPRFRYDQVLMFAWKFMFPLALANLCVTAVAVWALRA"
     gene            990..1571
                     /locus_tag="VFV55_09130"
     CDS             990..1571
                     /locus_tag="VFV55_09130"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX4944505.1"
                     /translation="MNKKTHVRKQYWNEPEMGLWERAYLPEILRGLFITGNVFVRNMV
                     KWMTGRKGALTTYYPEETRWDYAAANRGKHILTQRPDGSPQCIACNMCATVCPAKVIE
                     IEAAFDPADAAHPKYPARFEIDYSRCIFCGLCVEACPEDAIRMVKEVPNLPATDRWDM
                     WLGKEELLQWKPQGDVAKPYPPAPPRQPKGGKA"
     gene            1568..2101
                     /locus_tag="VFV55_09135"
     CDS             1568..2101
                     /locus_tag="VFV55_09135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX4944506.1"
                     /translation="MIRDLLANLDTLLVWAFAGCAFVSASLMLVLRQPMRVALALVSC
                     MIFLGGVYGLLGVHFIAAFQVLIYVGAVMVFMVYAIMLLDPRDEAASTAKYSKFLWPG
                     IIGFALLAEALAVGYWNDLPPAPRGAAGEAFGISRFSATFLSDYWLHFELTSVLLLAA
                     VVAALAVIKENRRHDRG"
     gene            2094..2396
                     /gene="nuoK"
                     /locus_tag="VFV55_09140"
     CDS             2094..2396
                     /gene="nuoK"
                     /locus_tag="VFV55_09140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX4944507.1"
                     /translation="MDKVQMLQVLAASLFALGLTGVIIRRNVLVMLMCMELMLNGVNL
                     SLVTFAYMLGSAVGAVLVFLIFVVATAEIAIAIPVVLLLVRRKRTLDIESYSDLKG"
     gene            2403..4280
                     /gene="nuoL"
                     /locus_tag="VFV55_09145"
     CDS             2403..4280
                     /gene="nuoL"
                     /locus_tag="VFV55_09145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX4944508.1"
                     /translation="MGLLTLIVVLPVIGFLVNGLAGNRMGKGFVTAVGCGLPAIAFVA
                     AIKCFIDLRAGGWAPLAETAYIWSMIGPDTFEVAFYFDRLTAVMALIVTGVGTLIHVY
                     SIGYMHDDKSYARYFAYLNLFLFFMLLLVLGKSLLVLFVGWEGVGLASYLLIGFWFGD
                     MEKAAAGKKAFIANRIGDAGFLLGMFVLYSALGTLDMPRINAAFMGASVPAVSASLVG
                     ILLFIGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGIYLVARMSGIYLHAP
                     EASTIIATVGALTALMAATIALAQDDIKKVLAYSTVSQLGFMFVALGVGAYGVAIFHV
                     TTHAFFKACLFLGAGSVIHAMSGEQDIQKMGGLGRKIPVTFVTFAIATAAIAGIPPLS
                     GFFSKDEILWYTLASDKGGSLLLFAVMAFTALLTAFYMFRLLWLTFFGKPRMIKEVEH
                     HIHESPASMTGVLVVLAALSAVGGFLSIPHFLEPMLPLPGISHEMHAFHYWVVGGSIA
                     IAAAGLAGAAWFFAGDSSRADQVKASFAGVYRVLSHKYYVDEFYDLVINRPLIWVSDT
                     IFLKLGDRMLIDGTLNGLASLAQRAAGRLSLVQTGSLHKYAILVLAGMVASLVWMWRH
                     V"
     gene            4273..5844
                     /locus_tag="VFV55_09150"
     CDS             4273..5844
                     /locus_tag="VFV55_09150"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX4944509.1"
                     /translation="MSDATVLSLVLYLPVAGMLAIAAIPAGRDGAVRTFTLAVMVAQF
                     LLSVILYAHFDPAIPGLQAETRLPWIPEWGVNYIIGLDGYNILLVMLTTFLGPLVTAG
                     AFSAITKEVKLFYAMVFLIQFAMLGAFVAQDLFVFYLFWETMLIPMFLIIGIWGGERR
                     IYATLKFFLYTAFGSILMLAAVIYLVYSLWHATGITSFAFADLYKARLPLDAQQWLLA
                     AFALSFAIKVPMVPFHTWLPDAHVEAPTPGSVILAGVLLKMGTYGFMKLGFPLFPDAT
                     RLATPLLSSLAVVSIVYGACLALVQKDIKKIIAYSSISHLGYVMLGLVSLDLLGIQGA
                     VVQMVSHGLVAGGLFLMVGMIYERCHTRELAAYGGLAKVLPVYSVFFLILTLASVGLP
                     TTSGFTGEFLVLMGAFNAAWRESAGGSTYAIWVAGAAVSGVVLGALYMLWFAQRFLFG
                     PERVPHGPVTDLNLREKSILALIVAAVFWLGLFPDSAMKRTELAAREYQQLVATPRMP
                     AATKFTGAVPAEAVR"
     gene            5841..>6163
                     /locus_tag="VFV55_09155"
     CDS             5841..>6163
                     /locus_tag="VFV55_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944510.1"
                     /translation="MNFTAVSIAMAPEHLLLAGIVALLVLDIVAERSRAALPLAVLFV
                     VASTVAALILGFNGWTGAPFPGQFVLTPPASFAKALVLALAVPVLMFSKDDFGEGAFY
                     PLVLSS"
BASE COUNT          832 a         2134 c         1981 g         1216 t
ORIGIN      
        1 atcccgttca cgcaggtgaa gctggtctgg tggggactct tccacggcat ggccgacggc
       61 ctgaagatgc tgctcaagga ggacttcaag ccgcgcacct acgactggta cgcctacgcg
      121 gtggcgccct gggtggtgtt cacgccggtg ctcctggtct tcgcggtgat cccgttcggc
      181 ggcacgctct ctcccgcaca gctctgggcg ccgctcgcgc agtggttcgg tgacaagagc
      241 tacccgatgc agatcgccaa gctggacgcg gggctgctca tggtgttcgc cttcagcggc
      301 ctcaccgtga tcggcgcgat gctcgccggc tggtcgtcct ccaacaagtt ctcgctgctc
      361 ggcgcgctgc gcgccgggtc gcagatgatc tcctacgagc tggtgctggg cctcaccgtg
      421 ctcggcctca tcctcatcta cggcaccgtg gacctggacg tgatggtgcg ccaccagtcg
      481 ggcaccgtgc tcgggttcct gcccgcctgg ggcatcgtct accagccctt cgcggccacc
      541 ctgttccttg ccgccgccat cgcggagaac aagcgcatcc ccttcgacct gccggaggcg
      601 gagtccgagc tgatcgccgg ctactacacc gagtacagcg ccatgaagat gggcctcttc
      661 atgttcgcgg agttcatcga gatcgcgatc gtcggcgcgc tcttcaccac cctgttcctc
      721 ggtggataca accttccgta catgtcggat gccgggttcg ccttcccggg cggcggttcc
      781 gtcgcgctgc cgcacgccgc agtggtcttc acgcagctcg cggtgttcct cctcaaggtc
      841 ttcgtcgtgt gcagcttcca gatcctcatc cgctggacgc tgccgcgctt ccgctacgac
      901 caggttctga tgttcgcctg gaagttcatg ttcccgctgg cgctcgcgaa cctgtgcgtg
      961 accgccgtgg ccgtctgggc gctgcgcgca tgaacaagaa gacacacgtc cgcaagcagt
     1021 actggaacga gccggagatg gggctgtggg aacgcgccta cctgcccgag atcctgcgcg
     1081 gcctgttcat caccggcaac gtgttcgtgc gcaacatggt caagtggatg accgggcgca
     1141 agggagcgct gaccacgtat taccccgagg agacgcgctg ggactacgcc gccgccaacc
     1201 gcggcaagca catcctcacg cagcgacccg acggctcgcc gcagtgcatc gcctgcaaca
     1261 tgtgcgcgac cgtctgcccg gccaaggtga tcgagatcga ggcggccttc gatcccgccg
     1321 acgcggcgca cccgaaatac ccggcgcgct tcgagatcga ctactcgcgc tgcatcttct
     1381 gcggcctgtg cgtggaagcc tgtcccgagg acgcgatccg catggtgaag gaagtgccga
     1441 acctgcccgc caccgaccgc tgggacatgt ggctgggcaa ggaagagctg ctgcagtgga
     1501 agccgcaggg cgacgtggcc aagccctacc cgccggcgcc gccgcgccag ccgaagggag
     1561 gcaaggcatg atccgcgacc ttctcgccaa cctcgacacc ctgctcgtgt gggcgttcgc
     1621 cggctgcgcc ttcgtgagcg cctccctcat gctcgtgctg cgccagccga tgcgcgtggc
     1681 gctggccctc gtctcgtgca tgatcttcct cgggggcgtg tacgggctgc tgggcgtgca
     1741 cttcatcgcc gccttccagg tgctcatcta cgtgggcgcg gtgatggtgt tcatggtcta
     1801 cgcgatcatg ctgctcgacc cgcgcgacga ggccgcctcg accgcgaagt actcgaagtt
     1861 cctgtggccc ggcatcatcg gcttcgccct gctggccgag gcgctcgcgg tgggctactg
     1921 gaacgatctc ccgcccgcac cgcgcggcgc cgcgggcgag gccttcggca tctcgcggtt
     1981 ctcggcgacg ttcctgtcgg attactggct gcacttcgag ctcacctcgg tgctgctgct
     2041 ggcggccgtg gtggcggcgc tggccgtgat caaggaaaac cggaggcacg accgtggata
     2101 aggtgcagat gctgcaggtc ctcgcggcgt cgctcttcgc gctgggcctc acgggcgtga
     2161 tcatccggcg caacgtcctg gtgatgctga tgtgcatgga actcatgctc aacggcgtga
     2221 acctgagcct cgtcaccttc gcctacatgc tgggatcggc ggtgggcgcg gtgctcgtgt
     2281 tcctgatctt cgtggtggcc acggccgaga tcgcgatcgc gatccccgtc gtgctgctcc
     2341 tcgtgcggcg caagcgcacg ctcgacatcg agtcctactc ggacctcaag ggctagcgcg
     2401 caatgggact cctcaccctc atcgtcgtcc tcccggtcat cggcttcctg gtcaacgggc
     2461 tggccggcaa ccggatgggc aagggcttcg tcaccgcggt cggctgcggc ctgccggcca
     2521 tcgccttcgt ggcggcaatc aagtgcttca tcgacctccg ggcgggcggc tgggcgccgc
     2581 tggccgaaac cgcctacatc tggtcgatga tcgggccgga caccttcgag gtcgcgttct
     2641 acttcgaccg cctgaccgcg gtgatggcgc tcatcgtgac gggcgtgggc accctgatcc
     2701 acgtctattc catcggctac atgcacgacg acaagagcta cgcgcgctac ttcgcgtacc
     2761 tcaacctgtt cctcttcttc atgctgctgc tggtgctggg caagtcgctg ctggtgctct
     2821 tcgtcggctg ggagggcgtg gggctcgcgt cctacctcct catcggcttc tggttcggcg
     2881 acatggagaa ggccgcggcg ggcaagaagg ccttcatcgc caaccgcatc ggcgacgccg
     2941 ggttcctgct cggcatgttc gtgctctatt ccgcgctcgg cacgctcgac atgccgcgga
     3001 tcaacgcggc attcatgggc gcttccgttc cggcggtgtc cgcgagcctc gtgggcatcc
     3061 tgctcttcat cggcgccacc ggaaagtcgg cgcagatccc gctctacgta tggctgccgg
     3121 acgcgatggc gggccccacg cccgtttccg ccctgatcca cgcggcgacg atggtgaccg
     3181 cgggcatcta cctggtggca cggatgtccg gcatctacct gcatgcaccg gaggcctcga
     3241 cgatcatcgc gacggtcggg gcgctcaccg cgctcatggc cgccaccatc gcgcttgccc
     3301 aggacgacat caagaaggtg ctcgcctact ccaccgtctc gcaactgggt ttcatgttcg
     3361 tcgccctcgg cgtgggcgcc tacggcgtgg ccatcttcca cgtcaccacg cacgcgttct
     3421 tcaaggcctg cctgttcctc ggcgcgggca gcgtcatcca cgccatgtcg ggcgagcagg
     3481 acatccagaa gatgggcggg ctgggccgca agatcccggt caccttcgtc accttcgcca
     3541 tcgcgaccgc ggcgatcgcg ggcatcccgc cgctctccgg cttcttctcc aaggacgaga
     3601 tcctgtggta cacgctggcc agcgacaagg gcggctcgct cctgctcttc gcggtcatgg
     3661 cgttcacggc gctgctcacc gcgttctaca tgttccggct gctgtggctc accttcttcg
     3721 gcaagccgcg catgatcaag gaggtcgagc atcacatcca cgagtcgccg gcctcgatga
     3781 cgggcgtgct cgtggtgctg gccgcgctgt cggccgtcgg cggtttcctg tcgatcccgc
     3841 acttcctcga gccgatgctg ccgctgcccg gcatcagcca cgagatgcac gcgttccact
     3901 actgggtggt gggcgggtcg atcgccatcg ccgcggcggg gctcgcgggc gcggcctggt
     3961 tcttcgccgg ggactcctcg cgcgccgacc aggtgaaggc aagcttcgcg ggcgtgtacc
     4021 gggttctttc gcacaagtac tacgtcgacg agttctacga cctcgtcatc aaccggccgc
     4081 tcatctgggt gtcggacacc atcttcctca agctgggtga ccggatgctg atcgacggca
     4141 cattgaacgg cctcgcgtcg ctggcacagc gggcggcggg ccggctctcc ctcgtgcaga
     4201 ccgggagcct gcacaagtac gcgatcctgg tgctcgccgg catggtggcg agcctcgtct
     4261 ggatgtggcg ccatgtctga cgcaacggtt ctttcgctgg tcctgtacct tcccgtcgcg
     4321 ggcatgctcg ccatcgcggc cattccggcg gggcgcgacg gggccgtgcg caccttcacc
     4381 ctcgcggtga tggtggccca gttcctcctc tccgtgattc tgtacgcgca cttcgatccg
     4441 gccattcccg gcctgcaggc cgagacgcgc ctgccgtgga tccccgagtg gggggtgaac
     4501 tacatcatcg ggctggacgg ctacaacatc ctgctggtga tgctcaccac cttcctcggc
     4561 ccgcttgtga ccgccggcgc cttcagtgcg atcaccaagg aagtgaagct cttctatgcg
     4621 atggtgttcc tgatccagtt cgcgatgctc ggcgccttcg tcgcgcagga cctgttcgtg
     4681 ttctacctgt tctgggaaac gatgctgatc ccgatgttcc tcatcatcgg catctggggc
     4741 ggcgagaggc gcatctacgc gacgctcaag ttcttcctct acaccgcctt cggcagcatc
     4801 ctcatgctgg cggccgtcat ctacctcgtg tattcgctgt ggcacgcgac cggcatcacg
     4861 tccttcgcct tcgccgacct gtacaaggcg cggcttccgc tcgatgccca gcagtggctg
     4921 ctcgcggcct tcgcgctgtc cttcgcgatc aaggtgccga tggtgccctt ccacacgtgg
     4981 ctgccggacg cgcacgtcga ggcgcccacg cccgggtcgg tgatcctcgc gggcgtcctg
     5041 ctcaagatgg ggacgtacgg cttcatgaag ctgggcttcc cgctctttcc cgacgccacg
     5101 cgcctggcca ccccgctcct gtcctcgctc gccgtggtga gcatcgtgta cggcgcctgc
     5161 ctggcgctcg tgcagaagga catcaagaag atcatcgcct actcctcgat cagccacctc
     5221 ggctacgtga tgctcgggct cgtgagcctg gacctcctgg gcatccaggg cgcggtggtg
     5281 cagatggtga gccacgggct ggtcgccggc gggctcttcc tcatggtggg catgatctac
     5341 gagcgctgcc acacgcgcga gctcgcggcc tatggcggcc tggcgaaggt gctgccggtg
     5401 tactcggtct tcttcctgat cctcacgctc gcctccgtcg ggcttcccac gaccagcggc
     5461 ttcaccggcg agttcctcgt gctgatgggc gccttcaacg ccgcctggcg cgagtcggct
     5521 ggcggatcca cctatgcgat ctgggtggct ggcgccgcgg tctcgggcgt ggtgctgggg
     5581 gcgctgtaca tgctctggtt cgcgcagcgc ttcctcttcg gccccgagcg cgtgccgcac
     5641 gggccggtga cggacctgaa cctgcgcgag aagtcgatcc tcgcgctcat cgtggcggcc
     5701 gtcttctggc tcgggctgtt ccccgattcc gcgatgaagc gcaccgagct cgcggcgcgc
     5761 gagtaccagc aactggtcgc cacgccgcgg atgccggcgg cgacgaagtt caccggggct
     5821 gtcccggcgg aggcggtgcg atgaacttca cggccgtatc gatcgccatg gccccggagc
     5881 acctgctgct cgcgggcatc gtggcgctgc tcgttctcga catcgtcgca gaacgctcgc
     5941 gcgcggcgct gccgcttgcg gtcctgttcg tggtcgcgtc gacggttgcg gcattgatcc
     6001 tcgggttcaa cggctggacg ggcgcgccgt tccccggcca gttcgtcctc acgccgccgg
     6061 catcgttcgc gaaggcgctc gtgctggcgc tcgcggtgcc ggtgctcatg ttctcgaagg
     6121 acgacttcgg cgagggcgcc ttctatccgc tcgtgctctc ctc
//
LOCUS       DASUEV010000285         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1216108, whole genome shotgun sequence.
ACCESSION   DASUEV010000285 DASUEV010000000
VERSION     DASUEV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216108"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /gene="ppdK"
                     /locus_tag="VFV55_09160"
     CDS             complement(<1..1844)
                     /gene="ppdK"
                     /locus_tag="VFV55_09160"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX4944511.1"
                     /translation="MTNRKYIFTFEEGDGKNKMLLGGKGANLCEMTQIGLNVPPGFAI
                     TTEACLAYLAKNQLPPGLMDDIRANMKLLEQKTGKTFGGGNPLLVSVRSGSAMSMPGM
                     MDTILNLGLNETSLEGLIRQTANERFAWDAYRRFIQLFGKIALGIADEHFDERMAAVK
                     RKYGARLDVDLDAVHLRELAGDFLEIVKSQTGKPFPQDPYEQLEISVGAVFRSWMGKR
                     AVDYRRQFRITPEMANGTAVNVCTMVFGNMGNDSGTGVGFTRNPGTGESAIYGEYLVN
                     AQGEDVVAGIRTPRPIAEMEKEMPDVYRQLLELHNRLESHYHEVQDFEFTIEKGKLYC
                     LQTRNGKMNARAMVRTSVEMFGEGLISRERALLRVEPAVLEQLLVPQLSPDFHGKSLA
                     QGLPASPGAASGKIVFDANTAESRGHAGEKVILVREETKPDDIHGFFQAQGILTSRGG
                     KTSHAAVVARGMGKPCVSGCEQIVINDKARSATIGETTLHEGDVITIDGGTGHVYAGE
                     VPTVEAAFSGELVTLLAWADEVAQLKVMANADTPEDATRARQFGAVGIGLVRTERMFN
                     STDRLPIMQEMILAETREERQAALDRLLPIQRADFKGIFKAMKGLPVTV"
     gene            1973..3133
                     /locus_tag="VFV55_09165"
     CDS             1973..3133
                     /locus_tag="VFV55_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX4944512.1"
                     /translation="MLAAHFSRFLAANPGLLHFAAHSHHPWPDVTQAAHARYWEDSAT
                     LADRKWAHVFGTVLPAAQDHVARLLCISDPRQIAFAPNTHEFVARLYSCLDWSRPVRV
                     LASAHEFHSFRRQTRRLAETGRLVFSEIPAEPYATFDDRFRAAAASGDWDLVWLSHVF
                     FDSGFVVRDLERIVRAAPGNAIVAIDGYHAFHAFPVDLSSIRDRAFYLGGGYKYAMSG
                     EGACFLAIPPGCDLRPADTGWFASFDSLSGRPGMAVPYSRDAFRFWGSTFDASGLYRF
                     NAVMDWLAGLGVTVADIRAHAQRLQSRFLAALAGLALPRLPADRLVPPAGCSRGNFLT
                     FDIDGAEEVQQRLAAHGVHVDRRDRRLRFGFGVYHDEATVDALLEAVGKALR"
     gene            3144..4115
                     /locus_tag="VFV55_09170"
     CDS             3144..4115
                     /locus_tag="VFV55_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944513.1"
                     /translation="MKTLRVLAAAALVALAPLAGFAAEPFPGKQIRFVVPYPAGGPLD
                     TVARLLGQKVAASVGHPVLVDNVPGAGGNIGAGTVARAAPDGYTILMGAVATHAINPT
                     LYPAIPYNAEKDFIAVTQVASTPNILVVNPAMKAKSVAEFIELAKSQPGKLNFGSGST
                     GSAGHLAGELFKSMAGVDMVHIPYKGAAAAMQDLVGGRVDLMFDNLASSLSQVKGGRI
                     RALAVTTARRTPLAPELPTIAESGLPGFDINTWFGIFVPAGTPRPVVDRLHAEFTRAL
                     AAPDVREKMLNLGAEPIGSTPEAFAAYVKAEAARYAKIVKASGAKVD"
     gene            4125..4565
                     /locus_tag="VFV55_09175"
     CDS             4125..4565
                     /locus_tag="VFV55_09175"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HEX4944514.1"
                     /translation="MVRRIAWFLAVAGTVPFVLATVALYASDASSVRVPAITALVTYS
                     AVILSFLAGIEWGLAIRDESGTETTRVIALGLSSVSSLAAWAVLWLPSPSWQVGCALG
                     LFVAAWAADEWMAGRGLLPTWFVNLRTAVSVLVAANLSLVLFRL"
BASE COUNT          679 a         1657 c         1515 g          861 t
ORIGIN      
        1 accgtcacgg gcagcccctt catcgccttg aagatgccct tgaagtcggc gcgctggatg
       61 ggcaggagcc ggtcgagcgc cgcctggcgc tcctcgcgcg tctcggcgag gatcatctcc
      121 tgcatgatcg gcaggcggtc ggtgctgttg aacatgcgct cggtgcgcac cagcccgatt
      181 cccaccgcgc cgaattgcct ggcgcgcgtc gcgtcctccg gggtgtcggc gttggccatc
      241 accttgagct gcgccacctc gtcggcccag gcgagcagcg tgaccagttc gcccgagaac
      301 gcggcctcga cagtcggcac ttcgcccgcg tagacgtggc ctgtgccgcc atcgatggtg
      361 atgacgtcgc cctcgtgcag ggttgtctcg ccgatggtcg cgctgcgggc cttgtcgttg
      421 atcacgatct gctcgcaccc ggagacgcag ggcttgccca tgccgcgcgc gaccaccgcg
      481 gcgtgcgagg tcttgccgcc gcgactggtg agtatgccct gcgcctggaa gaagccgtgg
      541 atgtcgtcgg gcttggtctc ctcgcgcacg aggatgacct tctcgccggc gtggcctcgc
      601 gattccgcgg tgttggcatc gaacacgatc tttccggagg ccgcaccggg cgaggccggg
      661 aggccctgcg ccagggactt gccgtggaaa tccggcgaga gctgcggcac cagcagttgc
      721 tccagcaccg cgggctcgac ccgcagcagc gcgcgctcgc gcgaaatgag cccttcgccg
      781 aacatctcga cggacgtgcg caccatggcc cgcgcattca tcttgccgtt gcgagtctgc
      841 aggcagtaga gcttgccctt ttcgatcgtg aactcgaagt cctgcacctc gtggtaatgc
      901 gactccagcc ggttgtgcag ctccagcagc tggcggtaga cgtccggcat ttccttttcc
      961 atctcggcga tcggccgggg cgtgcggatt cccgccacca cgtcctcgcc ctgggcgttg
     1021 acgaggtatt cgccatagat ggcgctctcg ccggtcccgg gattgcgcgt gaagccgacg
     1081 ccggtgcccg aatcgttgcc catgtttccg aacaccatcg tgcacacgtt gaccgctgtg
     1141 ccgttggcca tttccggcgt gatcctgaac tgcctgcggt agtccaccgc gcgcttgccc
     1201 atccacgagc ggaacaccgc gcccacggag atctcgagct gctcataggg gtcctgcgga
     1261 aacggcttcc cggtctggct cttcacgatc tcgagaaaat ccccggcgag ctcgcgcaga
     1321 tgcacggcgt cgaggtccac gtccaggcgc gccccgtact tgcgcttcac ggccgccatg
     1381 cgttcgtcga agtgctcgtc ggcgatgccc agggcgatct tgccgaaaag ctggatgaag
     1441 cgccggtagg catcccaggc gaagcgctcg ttggcggtct gccggatcag cccctccagc
     1501 gaggtctcgt tgagcccgag gttgaggatg gtgtccatca tgccgggcat ggacatggcc
     1561 gagccggagc gcaccgagac gagcagcggg ttgccgccgc cgaaggtctt gccggtcttc
     1621 tgctccagca gcttcatgtt ggcgcggatg tcgtccatca ggccgggcgg aagctggttc
     1681 ttcgcgaggt aggcaaggca cgcctcggtg gtgatggcga accccggcgg cacgttcagg
     1741 ccgatctgcg tcatctcgca caggttcgcc cccttgccgc ccagcagcat cttgttcttg
     1801 ccgtcgccct cttcgaacgt gaagatgtat ttcctgttgg tcatggtccg ctccctggat
     1861 ggcgtgactt tacttgcaga acgccgcctt gcggaaggaa gttgttgacc ccggtcaaag
     1921 accggatggc ccgccttgcg cgcaccgccc cggcggcaca gaatcggcgg ccatgctcgc
     1981 cgcgcatttc tcccggttcc ttgccgccaa tccgggcctg ctgcacttcg ccgcgcacag
     2041 ccaccatccc tggcccgacg tcacgcaggc cgcccacgcg cgctactggg aagactccgc
     2101 cacgctcgcc gaccgcaagt gggcgcacgt cttcggcacc gtgctaccgg ccgcccagga
     2161 ccatgttgcg cggctgctct gcatttccga tccgcggcag atcgcctttg cgcccaacac
     2221 gcacgagttc gtggcacgcc tctactcctg cctcgactgg tcccgcccgg tgcgcgtcct
     2281 cgcgagcgcg cacgagttcc acagcttccg ccggcagacc cggcgcctgg cggagacggg
     2341 aaggctggta ttcagcgaga tccccgctga gccgtacgcg actttcgacg atcgcttccg
     2401 cgcggcggcg gcgtccggcg actgggacct ggtctggctg tcccatgtct tcttcgactc
     2461 gggttttgtc gtccgtgacc tggaacggat cgttcgtgcc gccccgggga acgcaatcgt
     2521 tgccatcgac ggctaccacg ccttccatgc atttcccgtc gacctctcgt cgatacggga
     2581 ccgcgccttc tatcttgggg gcggatacaa gtacgcgatg tcgggcgaag gcgcttgctt
     2641 tctcgcaatc ccgccagggt gcgacctgag gccggccgac accggctggt tcgcttcctt
     2701 cgattcgcta tccggcaggc ccggcatggc agtgccctac tccagggacg cgtttcgctt
     2761 ctggggatcg acattcgacg catcggggct ctaccgcttc aacgcggtga tggactggct
     2821 cgcgggcctg ggcgtcacgg tcgcggatat ccgcgcgcac gcgcagcgcc tgcaatcgcg
     2881 ctttctcgcc gccttggccg gtctcgccct cccccgcctt ccagcagacc gcctggtgcc
     2941 gccggccgga tgttcgcgag gaaacttcct cacgttcgat atcgacggcg cggaagaggt
     3001 gcagcagcgc ctcgcggccc acggcgtgca cgtcgaccgc cgcgaccgtc gccttcgctt
     3061 cggcttcggc gtgtatcacg acgaagcaac cgtggacgcc ctgctcgagg cggtcgggaa
     3121 ggccctgcga tagaatcgcg gccatgaaaa cgctcagagt ccttgccgcc gcggccctcg
     3181 tcgcgctggc gccgctggcc ggcttcgcgg ccgaaccatt ccccggcaag cagatccgct
     3241 tcgtcgtccc ctatcccgcc ggcggcccgc tcgacaccgt ggcccggctc ctcggccaga
     3301 aggtcgccgc gagcgtggga caccccgtgc tggtggataa cgttcccggt gccggcggca
     3361 acatcggggc gggtaccgtc gcgcgggcgg cgccggacgg ctacaccatc ctcatgggcg
     3421 cggtcgccac gcacgcgatc aatccgacgc tctacccggc catcccgtac aacgcggaaa
     3481 aggacttcat cgccgtcacg caggtcgcct ccacgcccaa cattctggtc gtgaatcccg
     3541 cgatgaaggc gaaatcggtg gcggagttca tcgagctcgc gaaatcgcag ccgggcaagc
     3601 tcaatttcgg ctccggcagc actggcagcg cagggcacct ggcgggcgag ctcttcaagt
     3661 cgatggcggg cgtcgacatg gtccatattc cctacaaggg cgcggccgct gcgatgcagg
     3721 acctggtcgg cggacgggtc gacctcatgt tcgacaacct cgcctcctcg ctttcccaag
     3781 taaagggcgg gcgcatccgc gcgctggcgg tcaccacggc gagacgcacc ccgctcgcgc
     3841 cggaactgcc gaccatcgcc gaatccgggc tgcccggttt cgacatcaat acgtggttcg
     3901 ggatcttcgt tccggccggc acgcccaggc ccgtcgtcga tcgactgcac gccgagttca
     3961 ccagggcgct cgccgcgccc gacgtgcgcg agaagatgct caatctcggc gccgagccga
     4021 tcggcagcac gcccgaggcg ttcgccgcct acgtgaaggc ggaggccgcc aggtacgcga
     4081 agatcgtcaa ggcctccggg gcgaaggtcg actgagccgc cgccatggtc cgacgaattg
     4141 cgtggttcct tgccgtcgcg ggcacggtcc ccttcgtgct cgcgaccgtc gcactctacg
     4201 cttcggacgc ttccagcgtg cgcgtgccgg cgatcaccgc actggtcacc tattccgccg
     4261 tgatcctgtc cttcctcgcg ggcatcgaat ggggcctcgc gatccgcgac gaatcgggaa
     4321 ccgaaaccac gcgcgtgatc gcgctgggcc tcagctccgt gtcatcgctc gcggcgtggg
     4381 ccgtgctgtg gcttccctcg ccctcgtggc aggtcggctg cgcgctcggg ctcttcgtcg
     4441 ccgcgtgggc cgccgacgag tggatggccg gccgggggct cctgccgaca tggttcgtca
     4501 acctgcgcac tgccgtgagc gtcctggtcg cggcaaacct ctcgctcgtc ctgttccgtc
     4561 tctagccgcg catcatttgg ttcttcaccg aattccgggg cgggaggcgg tggcggtgga
     4621 agtcgatcgc aatcctgggc acgcaatcgt gaccgtcgga tgggtgcccg gaaagtcgag
     4681 gccagccaaa cacgtctgtc ctcgactttc ca
//
LOCUS       DASUEV010000286         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_413531, whole genome shotgun sequence.
ACCESSION   DASUEV010000286 DASUEV010000000
VERSION     DASUEV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413531"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFV55_09180"
     CDS             complement(<1..785)
                     /locus_tag="VFV55_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX4944515.1"
                     /translation="MAFTDKDVLAALSELVDPVTGRNYVEGRCVKNVKVDGDRVSLDI
                     VLGYPARGVIEKVRSQVTDRLKQLPGVVGASANVTSKIVSHAVQRGVKLVPGIKNIIA
                     VASGKGGVGKSTTAVNLALALAAEGATVGMLDADIYGPSQPTMLGITGRPTSADGKSM
                     EPMEGHGIQAMSVGFLIEADTPMVWRGPMVTQALEQLLTETRWRDLDYLVVDLPPGTG
                     DIQLTLAQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVGVPIL"
     gene            960..3101
                     /gene="metG"
                     /locus_tag="VFV55_09185"
     CDS             960..3101
                     /gene="metG"
                     /locus_tag="VFV55_09185"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX4944516.1"
                     /translation="MPRKLFVTTALPYANGPFHIGHIMEYTQADIWVRFQRLQGHKVH
                     FVCADDTHGAPIMLKAEAEGITPEELIARVAESHRRDLERFGVSFDHFHSTHSEENRI
                     LAGEIFAALREAGFIATKTIEQFFDPVKGMFLPDRYIKGECPKCGAKDQYGDACENCS
                     AVYAPTDLKNPFSALTGATPVLKKSEHFFFRLSDPRCVEFLKAWTQDGKLQPEVSNKV
                     LEWLDGGLNDWDITRDAPYFGIEIPGVKGKYFYVWLDAPIGYLASLKAYLEKKGLDFA
                     KVLADPRLEQIHFIGKDIIYFHTLFWPAMLKFSGRKVPDNVYVHGFLTLTGEKLSKSR
                     GVTVSPQKYAELGLNPEWLRYYLAAKLNSRVEDLDFNPDDFLARVNSDLVGKYVNIAS
                     RAAGFISKRFNGKLSEEPLSAGRELKHKMRGSVAAPVPDNEEQVPAILFEVRSFADQV
                     AADYEARDFGKAIRQVMFLADRVNQYVDEQKPWEIAKEPGQEAALQWFCTLYLELFRI
                     ITIYLKPVLPRVAEDVEAFLALTRPLSWEDVASALKPGHTVQPYRHLLSRIDPKQIDQ
                     LLEPPDEAKPEAAKPAAGGAPEFRLEPVEPPRAAPILPVGETISIDDFGKIDLRVARI
                     VDAELVADADKLLKLTLDIGIDTRTVFAGIRSAYTPKDLVGRLTVMVANLAPRKMRFG
                     ESQGMVLAAGDGSSIYLLAPESGATPGMRIK"
     gene            3124..3495
                     /locus_tag="VFV55_09190"
     CDS             3124..3495
                     /locus_tag="VFV55_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX4944517.1"
                     /translation="MARRTPLLPPPYTTDRDALEREVVVEVFRASGPGGQHVNKTESA
                     LRLVHPPSGVVVIAQDSSSQHRNREIAYERLIERLVRLNYVPKTRVATKPTRSSRERR
                     IGEKKQRGVTKRTRGKVGGDE"
     gene            3488..3769
                     /locus_tag="VFV55_09195"
     CDS             3488..3769
                     /locus_tag="VFV55_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX4944518.1"
                     /translation="MNEPDCRRLRIHGLVQGVGFRYSMQREALRLGLNGWVRNRRDGS
                     VEAVVSGAASAVEAIVAWSRRGPPSAQVERVEVSIDEGRFDTFEQGPTA"
     gene            complement(3792..>4824)
                     /locus_tag="VFV55_09200"
     CDS             complement(3792..>4824)
                     /locus_tag="VFV55_09200"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX4944519.1"
                     /translation="ANGLQVLPPDVNEGGYRFAPLDVRRIRSGLGAVKGTGEQAIANI
                     VAAREAGGPFRDLGDFCRRVDRRIVNRRAMEALVKSGAFDAIEPNRASLAASLGAALE
                     LADQAERYAQQTSLFGGAEEATAEAFELMRVPDWPERERLLAEKQSLGFYLSGHPFSE
                     WRDEIRRVARTPLANLQPANEPQMAAGVIYGVAMRNSRRGRMAVLTLDDGSARVEVVV
                     FGELFHEKRAVIQEDQVVVVQGRVSQDEFSGGIRFTADKLMDLAEIRSQYARTIRLSI
                     NGQADSAKLRSLLAPYTGGKCAVAIHYRNAKGECDIRLPDEYRVKVSAPLLASLSEWL
                     TERNVEVMY"
BASE COUNT          788 a         1693 c         1589 g          754 t
ORIGIN      
        1 aggatgggca cgccgacctt ctcgaacatc ttgaggccct tcctggcatc gatcagggcg
       61 atgtcctggg gggtggtgac gatcaccgcc ccggtcaccg gcaccttctg ggcgagcgtg
      121 agctggatgt cgcccgtgcc cggcggcagg tcgaccacga ggtagtcgag gtcgcgccag
      181 cgcgtctcgg tgaggagctg ctcgagggcc tgcgtgacca tcgggccgcg ccagaccatg
      241 ggagtgtcgg cctcgatgag gaagccgacg ctcatcgcct ggatgccgtg gccctccatc
      301 ggctccatgg acttgccgtc cgccgaggtc ggccgcccgg tgatgccgag catggtcggc
      361 tgcgaggggc cgtagatgtc ggcatcgagc atgccgaccg tcgctccctc ggccgcgagt
      421 gccagcgcga ggttcacggc cgtcgtgctc ttgccgacgc cgcccttgcc cgaggcgacg
      481 gcgatgatgt tcttgatgcc cggaaccagc ttcacgccgc gctgcacggc gtgcgagacg
      541 atcttcgagg tcacgttggc gcttgcgccc accacgccag gcagctgctt gagacggtcc
      601 gtcacctgcg agcggacctt ctcgatcacg ccgcgggccg ggtagccgag cacgatgtcc
      661 agcgagacgc ggtcaccatc gaccttcacg ttcttgacgc agcgcccttc cacgtagttg
      721 cggcccgtga ccgggtcgac gagttcggat agagcagcga ggacgtcctt gtcggtgaat
      781 gccatgtcgg gaggtggcgc tcggcaagcg aacgcggaag cggaagggaa gcatgcaatc
      841 ttaaagcaat ttgctacaat cgcgttgacc catatcacgc cgatttcaat gcactccccg
      901 ccgcgtcgcg cggcggcccg agccgtccgc caaccactat aggctcgcca cccggcttca
      961 tgccacgcaa gcttttcgtc acgacggcgc ttccctacgc caacggcccc ttccacatcg
     1021 gccacatcat ggagtacacc caggccgaca tctgggtgcg gttccagcgg ctgcaggggc
     1081 acaaggtgca tttcgtctgc gccgacgaca cccatggcgc cccgatcatg ctcaaggcgg
     1141 aggccgaggg catcacgccc gaggagctga tcgcgcgcgt ggccgagtcg caccggcgcg
     1201 acctcgagcg cttcggcgtg agcttcgacc acttccattc cacgcactcg gaggagaacc
     1261 ggatcctcgc cggcgagatc ttcgccgcgc tccgggaggc gggcttcatc gccaccaaga
     1321 cgatcgagca gttcttcgat ccggtgaagg gcatgttcct gccggaccgc tacatcaagg
     1381 gcgagtgccc caagtgcggg gccaaggacc agtacggcga cgcctgcgag aactgcagcg
     1441 ccgtctatgc gcccacggac ctgaagaacc cgttctcggc gctcaccggc gccacgcccg
     1501 tgctgaagaa gtccgagcac ttcttcttcc ggctctcgga cccgcgctgc gtggagttcc
     1561 tgaaggcctg gacgcaggac ggcaagctgc agccggaggt ctcgaacaag gtgctcgagt
     1621 ggctcgacgg gggactgaac gactgggaca tcacgcgcga cgccccctac ttcggcatcg
     1681 agatcccggg ggtcaagggc aagtacttct acgtctggct cgacgccccg atcggctacc
     1741 tcgcctcgct gaaggcctac ctcgagaaga aggggctgga cttcgccaag gtcctggccg
     1801 accccaggct cgagcagatc catttcatcg gcaaggacat catctacttc cacacgctct
     1861 tctggccggc gatgctgaag ttctccggcc ggaaggtgcc cgacaacgtc tacgtgcacg
     1921 gcttcctcac cctcaccggc gaaaagctct ccaagtcgcg cggcgtgacg gtgagcccgc
     1981 agaaatacgc cgagctgggc ctgaacccgg agtggctgcg ctactacctc gccgcaaagc
     2041 tcaactcgcg cgtcgaggac ctcgatttca atcccgacga cttcctcgcg cgggtgaatt
     2101 ccgacctggt cggcaagtac gtgaacatcg cgagccgcgc ggcgggcttc atctccaagc
     2161 gcttcaacgg caagctttcc gaggagcccc tctccgcggg ccgcgagctc aagcacaaga
     2221 tgcgcggcag cgtggctgcg cccgttcccg acaacgagga gcaggtcccg gccatcctct
     2281 tcgaggtgag gagcttcgcc gaccaggtcg cggccgacta cgaggcgcgc gatttcggca
     2341 aggccatccg ccaggtgatg ttcctcgccg accgcgtgaa ccagtacgtg gacgagcaga
     2401 agccctggga aatcgcgaag gagccgggcc aggaggcggc gctgcagtgg ttctgcacgc
     2461 tctatctcga gctcttccgc atcatcacca tctacctcaa gcccgtgctg cccagggtcg
     2521 ccgaggacgt cgaggccttc ctcgcgctca cccggccgct ttcgtgggag gacgtcgcca
     2581 gcgctctcaa gccggggcac acggtgcagc cctacaggca cctgctctcg cgcatcgacc
     2641 cgaagcagat cgaccagctg ctcgagcccc cggacgaggc gaagccggaa gccgccaagc
     2701 ccgccgccgg cggggcgccc gagttccggc tggaacccgt ggagccgccg agagcggcgc
     2761 ccatcctgcc ggtgggcgag acgatctcga tcgacgactt cggcaagatc gacctgcgcg
     2821 tggcccgcat cgtcgacgcg gaactggtcg cggacgccga caagctcctc aagctcacgc
     2881 tcgacatcgg catcgacacg cgaaccgtct tcgccggcat ccgctcggcg tacacgccca
     2941 aggacctcgt cgggcgcctc acggtgatgg tcgcgaacct ggcgccgcgc aagatgcgct
     3001 tcggcgaatc gcagggcatg gtgcttgccg cgggcgacgg cagttccatc tacctcctcg
     3061 cgcccgagtc gggtgcaacg ccggggatgc gcatcaagta atcagggaca gtccccggtt
     3121 tcaatggccc gccgcacgcc cctccttcca ccgccctaca ccaccgatcg cgacgccctc
     3181 gagcgcgagg tggtggtcga ggtgttccgc gcgagcggcc ccggcggcca gcacgtgaac
     3241 aagacggagt cggcgttgcg cctcgtccac ccgccctcgg gcgtcgtggt gatcgcgcag
     3301 gactcgtcct cccagcatcg caatcgcgaa atcgcgtacg agcgcctgat cgagcggctc
     3361 gtgcgcctca actacgtccc gaagacgcga gtcgcgacca agccgacgcg ctcttcgcgc
     3421 gagcggcgca tcggcgagaa gaagcagcgc ggcgtcacca aacgcacgcg tggcaaggtg
     3481 ggcggcgatg aatgaaccgg actgccggcg cctgcgcatc catggcctcg tgcagggcgt
     3541 gggcttccgc tattcgatgc agcgggaggc gctgcggctc ggcctcaatg gctgggtgag
     3601 gaatcggcgt gacggcagcg tggaggccgt ggtatccggg gccgccagcg ccgtggaggc
     3661 catcgtcgcc tggtcgcgcc gcggcccgcc gtcagcgcag gtggagcgtg tcgaggtttc
     3721 gatcgacgaa ggccgcttcg acaccttcga gcaaggcccc accgcctgag aaccagggac
     3781 agtccctgat ttcagtacat gacctcgacg ttgcgctcgg tgagccactc cgagagcgag
     3841 gcgagcagcg gcgcggacac cttcacgcgg tactcgtccg gcaggcggat gtcgcattcg
     3901 ccctttgcgt tgcggtagtg gatggccacc gcgcacttgc cgcccgtgta gggcgccagg
     3961 agcgagcgca gcttggcgga atcggcctgc ccgttgatcg aaagccggat cgtccgcgcg
     4021 tactggctgc ggatctcggc caggtccatc agcttgtcgg cggtgaagcg gatcccgccg
     4081 gaaaactcgt cctgcgacac gcgcccctgc accacgacga cctggtcctc ctggatcacg
     4141 gcgcgcttct cgtggaagag ctcgccgaag accaccacct cgacacgggc gctgccgtcg
     4201 tccagggtga gcacggccat gcgcccgcgg cgcgagttgc gcatggccac gccgtagatc
     4261 acgcccgcgg ccatctgcgg ttcgttggcg ggttgcaagt tcgccagcgg cgtcctcgcc
     4321 acgcgccgga tctcgtcgcg ccattcgctg aacgggtgcc cgctcaggta gaaccccagg
     4381 ctctgcttct cggccagcag ccgctcgcgc tccggccagt cgggcacgcg catgagctcg
     4441 aaggcctcgg cggtcgcctc ctccgcgccg ccgaacaggc tcgtctgctg cgcgtagcgc
     4501 tccgcctggt cggcgagctc gagcgcggcg cccagcgagg cggcgaggct cgcgcggttc
     4561 ggctcgatcg cgtcgaaggc acccgacttg acgagggcct ccatcgcgcg ccggttcacg
     4621 atgcgccggt cgacgcggcg gcagaagtcg cccaggtcgc ggaagggccc gcccgcctcg
     4681 cgggcggcca cgatgttcgc gatcgcctgc tcgcccgtgc ccttcaccgc ccccagcccg
     4741 gagcggatgc gccgcacgtc gaggggcgcg aagcggtagc cgccctcgtt cacgtccggg
     4801 ggcagcacct gcagcccgtt ggcg
//
LOCUS       DASUEV010000287         4435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_413823, whole genome shotgun sequence.
ACCESSION   DASUEV010000287 DASUEV010000000
VERSION     DASUEV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413823"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFV55_09205"
     CDS             <1..339
                     /locus_tag="VFV55_09205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX4944520.1"
                     /translation="VVDKAVFRTERGVGAGYYITVVVCEIVFGILASIIVAWFSRQRE
                     FRADAGAARFMGTPRPMVSALRRLGGLEPGELPKAFQSSGISDKPGFMALFSTHPPIE
                     ARIAALESRG"
     gene            336..1502
                     /locus_tag="VFV55_09210"
     CDS             336..1502
                     /locus_tag="VFV55_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4944521.1"
                     /translation="MTAGSPVGRRPIRLAWVVVLTVFGHAAFNGSRVTVSLYALSLQS
                     SPLTVGALVSLYSLLPIFLSVSAGRMIDRVGTTRPLLWSSVALACGVGLPALFPGLPP
                     LYLASAIIGLAFMVFHIAIQNSVGALSGPEDRAVNFSWLALGFSISGFLGPTTSGLAI
                     DNAGYRATFAILASSAIIPALALAIAKPWIPRAHAGRAPGGRLADLLGKRDLRRVFVV
                     TGILAMAWDLFFFVMPIYGTSIGLSASTIGGVLGSFALATFLVRLVLPWFARQLPEWR
                     LVSATMFVACAAYALFPLVRTVPLIAAIAFLLGLGLGASQPSLMSLIQDATPQGRLGE
                     ALGVRTTVMNMSHTVLPLFFGGIGTALGMGPVFWSMSIALGVAGVFAHRHRPGS"
     gene            1582..1905
                     /locus_tag="VFV55_09215"
     CDS             1582..1905
                     /locus_tag="VFV55_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX4944522.1"
                     /translation="MPIYEYRCSNCGHQQEFLQKVSDAPQTRCTECGQETFSKMVTAA
                     GFHLKGSGWYATDFKGSGSKPSAKAEGGSESKPAAKAEGTPESKSDSKGPAPACGSGS
                     CPACQ"
     gene            1960..2616
                     /locus_tag="VFV55_09220"
     CDS             1960..2616
                     /locus_tag="VFV55_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX4944523.1"
                     /translation="MRRYLIAGLLVWIPLAITLWVLSLLVELMDQSLMILPNRYRSDA
                     LLGFHLPGLGAVLTLAILLATGAIAANFFGRRMLLFSESILGRIPIVRSIYGGVKQIS
                     DTLFSPDGKAFRRAVLVRYPHAGAWTVALVTGTPEHEVTDHLGHEQVSVFVPTTPNIT
                     AGFFLIVPRTDTIELRMSVDQALKYIISMGVAEPPRPWDRPESLASGTDPNPVPSPKT
                     "
     gene            2729..>4435
                     /gene="aspS"
                     /locus_tag="VFV55_09225"
     CDS             2729..>4435
                     /gene="aspS"
                     /locus_tag="VFV55_09225"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX4944524.1"
                     /translation="MRTEYCGLIDTRFLGQTVTLFGWAHRRRDHGGVIFIDVRDREGL
                     VQVVSDPDRKETFANADRVRNEFVLRVTGVVRRRPEGTVNPGLRSGEIEVLAHELEVL
                     NPAVTPPFMMDDETLSEEVRLRYRYLDLRREPMQKAMQLRHRTAKAVRDYLDDNGFID
                     IETPVLYKSTPEGAREFLVPSRIHDGQFYALPQSPQLFKQLLMIAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDIETSFLSEREIQDIMEGLVRHVFKRVLDVDLPAFPRLAYADA
                     MAKYGSDKPDLRVKLELTELTDLMKGVDFKVFRAAAELPDGRVAALRVPGGNAAFTRK
                     ELDDLAPFVAIYGAKGLAYIRVNDRTKPSDEGLQSPIVKFFKPAELAAILERTGAKDG
                     DVIFFGADRRKVVNDAMGALRVKVGHEKGHAESGWRPCWVVDFPAYEYDEENKRWGAA
                     HHPFTAPKDEHVALMDTDPGKVLAKAYDIALNGWEIGGGSVRIHRAEVQAKQFKTLGI
                     GDAEARAKFGFLLDALSYGAPPHGGIAFGFDRMVAMMAGVDQIRDVIAFPKTQRGQDL
                     MVE"
BASE COUNT          684 a         1550 c         1439 g          762 t
ORIGIN      
        1 gtggtcgaca aggccgtctt caggaccgag cgcggcgtgg gcgccggcta ctacatcacc
       61 gtcgtcgtct gcgagatcgt attcggaatc cttgcctcga tcatcgtcgc ctggttctcg
      121 cgccagcgcg aattccgggc cgatgcgggc gcggcccgct tcatgggcac accgcgcccc
      181 atggtctcgg cgctgcgccg cctcggcggc ctcgagcccg gggaacttcc caaggcgttc
      241 cagtcgtcgg gcatttccga caagcccgga ttcatggccc tcttctcgac gcatcccccc
      301 atcgaggcgc gcatcgccgc cctcgagtcg cgaggttgac cgcaggctct cccgtaggca
      361 ggcgacccat ccggcttgcg tgggtggtgg tgctcaccgt tttcgggcac gccgcgttca
      421 acggaagccg cgtcaccgtc tcgctgtacg cattgagcct gcagtcgagc ccgctcaccg
      481 tcggcgcgct ggtatccctc tattcgctgt tgccgatctt cctctcggtg agcgccgggc
      541 ggatgatcga ccgcgtgggc accaccaggc cgctcctgtg gtcgtcggtg gcgctggcat
      601 gtggcgtcgg gctccccgcc ctgtttccgg ggctgccgcc cttgtacctc gcgagcgcca
      661 tcatcgggct cgcattcatg gtgttccata tcgccatcca gaactccgtc ggggcgctca
      721 gcgggccgga agaccgcgcc gtgaatttca gctggctcgc cctcggcttc tccatctccg
      781 gcttccttgg ccccaccacg tcgggactcg ccatcgacaa tgccggctat cgggcgacgt
      841 tcgccatcct cgcctcgtcg gcaatcatcc ccgcgctggc cctggcgatc gcgaagccgt
      901 ggataccgcg cgcccatgcg ggcagggcgc cgggcgggcg actggcggat ctgctcggca
      961 agcgcgacct tcggcgcgtc ttcgtcgtca ccggcattct cgcgatggca tgggacctct
     1021 tcttcttcgt gatgcccatt tacggaacgt cgatcggcct ttccgcgtcg acgatcggcg
     1081 gcgtcctggg atcgttcgcg ctcgccacgt tcctggtgcg cctcgtgctt ccgtggttcg
     1141 cgcgccagtt gccggagtgg cggctcgtca gcgccacgat gttcgtcgcc tgcgcggcct
     1201 atgcgctctt cccgctggtc cggacggtac ccctcatcgc ggccatcgcc ttcctcctgg
     1261 gcctgggcct gggcgcctcg cagcccagcc tcatgtcgct catccaggac gcgacgccgc
     1321 agggccgcct gggcgaggcg ctcggcgtgc gcaccacggt catgaacatg agccatacgg
     1381 tgctgccgct ttttttcggg gggatcggca cggcgcttgg catgggaccg gtcttctggt
     1441 cgatgtccat cgcgctcggt gtcgccggcg tgtttgccca ccgccaccgg ccgggctcct
     1501 gaacgtcacc ggtgctgcat ccggtgcggc gcgccaagtg cttgtgttaa aatgacattt
     1561 tttccggatt ccgaggcgat catgccgatt tacgaatacc gctgctcgaa ttgtggccac
     1621 cagcaggagt tcctgcaaaa ggtgagcgac gccccgcaaa cgcgctgcac cgagtgtggc
     1681 caggagacgt tctccaagat ggtcaccgcc gcgggcttcc acctgaaggg cagcggctgg
     1741 tacgcgaccg acttcaaggg tagcggctcg aaaccgtccg caaaggccga aggcggttcc
     1801 gaatcgaagc ctgcggcgaa agccgaaggc accccggaat cgaaaagcga cagcaagggc
     1861 ccggcgcccg cttgcggctc cggctcgtgc ccggcctgcc aataggactt ccttgaccat
     1921 ttccaccccg gatcgatgcg cgggcaagta atggctatgc tgaggcgcta cctcatcgcg
     1981 ggcctgctgg tctggatccc gctcgcgatc accctctggg tgctctcgct gctggtcgaa
     2041 ttgatggacc agtcgctgat gatcctcccg aatcgctatc gcagcgacgc gctgctcggt
     2101 ttccacctgc cagggctcgg ggcggtgctc acgctcgcga tcctgctcgc caccggggcc
     2161 atcgccgcca atttcttcgg caggcgcatg ctcctcttct cggagtcgat cctcgggcgc
     2221 atcccgatcg tccgctcgat ctatggcggc gtgaagcaga tcagcgacac gctcttctcg
     2281 cccgacggca aggcgttccg gcgcgcggtg ctggtccgct atccgcacgc gggcgcgtgg
     2341 acggtcgcgc tcgtcaccgg gacgcccgag cacgaagtca ccgaccacct cggccacgag
     2401 caggtgtccg tcttcgtccc caccacgccc aacatcacgg cggggttctt cctcatcgtg
     2461 ccgcgaaccg acacgatcga gctgcgcatg agcgtcgacc aggcgctcaa gtacatcatc
     2521 tccatgggcg tggccgagcc gccgcgcccg tgggaccggc ccgagagcct cgcctccggg
     2581 accgacccca atccggtgcc gtcgccgaaa acgtaggcgg cggcgcaacg gcagcaccgg
     2641 acacttccac ggcagacacg gccggcctcc cgcagcgttg cgggagtgac gaatcgaatc
     2701 gccccgccga gcggggcaca gagagaacat gagaaccgaa tactgcggac tgatcgacac
     2761 ccgattcctc gggcaaaccg tgaccctctt cggctgggcg caccgccgcc gcgaccatgg
     2821 cggcgtcatc ttcatcgacg tgcgcgaccg cgaggggctc gtccaggtcg tgagcgatcc
     2881 cgaccggaag gagacgttcg ccaacgccga ccgcgtgagg aacgagttcg tcctgcgcgt
     2941 gacgggcgtg gtgcgccgcc gccccgaagg cacggtgaat ccggggctgc gcagcggaga
     3001 gatcgaggtg ctcgcccacg agctggaggt cctcaacccg gccgtcacgc ccccgttcat
     3061 gatggacgac gagacgctct ccgaggaggt gcgcctgcgc taccgctacc tcgacctgcg
     3121 ccgcgagccg atgcagaagg cgatgcagct gcgccaccgc accgcgaagg ccgtgcgcga
     3181 ttacctcgac gacaacggct tcatcgacat cgagacgccg gtgctctaca agtccacgcc
     3241 ggaaggcgcg cgcgagttcc tcgtgccctc gcgcatccac gacgggcagt tctacgccct
     3301 gccgcaatcg ccgcagctct tcaagcagct gctgatgatc gcgggcttcg accgctatta
     3361 ccagatcgtg aagtgcttcc gcgacgagga cctccgcgcc gaccggcagc ccgagttcac
     3421 gcagatcgac atcgagacct ccttcctttc cgagcgcgag atccaggaca tcatggaggg
     3481 gctcgtgcgc catgtcttca agcgcgttct cgacgtggac ctgcccgcgt tcccgcgcct
     3541 tgcctatgcc gacgcgatgg cgaagtacgg cagcgacaag ccggacctgc gcgtgaagct
     3601 cgagctcacg gagctcaccg acctcatgaa gggcgtggac ttcaaggtct tccgcgctgc
     3661 ggcagagctg cccgatggcc gtgtcgcggc cctgcgcgtg cccggcggca acgccgcatt
     3721 cacgcgcaag gaactcgacg acctcgcgcc cttcgtggcg atctacggcg ccaaggggct
     3781 cgcctacatc cgcgtgaacg atcgcacgaa gccgagcgac gagggactgc agtcgccgat
     3841 cgtgaagttc ttcaagccgg cggagctcgc ggcgatcctg gagcgtaccg gcgcaaagga
     3901 cggtgacgtg atcttcttcg gcgcggaccg gcgcaaggtg gtcaacgatg cgatgggggc
     3961 cctgcgcgtg aaggtgggcc acgagaaggg gcacgccgag tccggctggc gcccgtgctg
     4021 ggtggtcgat ttccccgcct acgagtacga cgaggagaac aagcgctggg gcgccgcgca
     4081 ccatccgttc acggcgccga aggacgagca cgtggcactc atggacaccg acccgggcaa
     4141 ggtgctcgcg aaggcgtacg acatcgcgct caacggctgg gagatcggcg gcggctcggt
     4201 gcgtatccac cgcgccgagg tgcaggcgaa gcagttcaag acgctcggca tcggcgacgc
     4261 ggaggcgcgc gccaagttcg gcttcctgct cgacgcgctt tcctacggcg cgcctccgca
     4321 tggcggcatc gccttcgggt tcgaccggat ggtcgcgatg atggcgggcg tggaccagat
     4381 ccgcgacgtg atcgcgttcc ccaagacgca acgcgggcag gacctgatgg tggag
//
LOCUS       DASUEV010000288         5676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1125413, whole genome shotgun sequence.
ACCESSION   DASUEV010000288 DASUEV010000000
VERSION     DASUEV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125413"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1643
                     /locus_tag="VFV55_09230"
     CDS             345..1643
                     /locus_tag="VFV55_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4944525.1"
                     /translation="MLKLFLRLYLFLMLPATAAFVGFMYLTDQVMAQVHAETQRSRAG
                     AAFDRAERIIGATDAPDREERLKEIEATFRIEHRILPMAQVADDWFMSRPEKERLAAG
                     GIAFRDRPGGGQVYLRKLPGGDRVLRIEWVGLYEYLALYYTIIVALVTLGLAVILLRW
                     MRPLYRDLESLTAAAARVGDGDLGARAAVGRASLVEPIAGAFNAMTERVQRLLESHRD
                     LEQGVAHELRTPLAQLKFDLELARESDTAAEREARFEAMGRDVADLEELVNELLLLAR
                     LREAPPYVPREIPARVLVEDVIRRARDEMRAAGREIAIDPPAALPETLICDGKFLARA
                     LVNVVRNAVRHATARVALSVEREGGRTVVHVDDDGPGIAPEDRERLFEPFTRAGAGRE
                     RETGGVGLGLAIVKSVAEWHGGGARIGPSPLGGARVSISW"
     gene            complement(1663..5655)
                     /locus_tag="VFV55_09235"
     CDS             complement(1663..5655)
                     /locus_tag="VFV55_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX4944526.1"
                     /translation="MPSLSIIPPDALATVLAELRPGLDLLGLPCCVVDTELRYRYVNP
                     GYPPYFGRTAGEMLGFTVSSAFSREPTDDRRKVLARALAGESLTFDRETLEGPNEGRW
                     VRAHYVPVRSGGAVVGVAIALVDIQHLKEAQSAIAARERQLSLITDTIGFPVTYIDSG
                     GIVRFANAQSAAWAGLRPADMVGRPMAQLAPPEIMSQSLPLLQRAWAGESVTYEREAL
                     WTGREKRRIRGHMIPDKDESGAVRGAIIVLMDIEEDHRLQESLLARTRELQLITENVG
                     VPMAYIGTDRRFRFANTPGLDWVPGVDTDNIIGMSLEEVYPPEVLAVVLEPLGKAMAG
                     EKVAYERQGTAPDGTRRWARVNLIPDLVAGEVHGVFVVVIDIDADRRLRDALERQERQ
                     LRFYAENIPEIVAFVDAGFRYQFVNKTFERLRGLAAADIIGRPVPEVLGEEAAAKYFT
                     PFVERLKGGETCTYERLLGRGDGNKRWFRMRLEPYMGDDGAFRGYYIVGTDVHDVKLA
                     QDRLADQEAKLRLYTDNIPVSVAYLDTQRRYVFVNRTFADTRGMKLEDIIGRTSAEVL
                     GEEAAAALTPMVSRVLSGQTATYERKMILPSGERRWILGRSVPDHGPDGKVRGMFVVG
                     HDVQELKDAQEQLRGREEELRFFAENIPEAIVYVDLERGCTFVNNVFLESRGLTREFA
                     LGKFPKDVYPPDLVQALQPYLDRVVTGETVTYERRMRVAGDGEERWVRVKLSPRKDAA
                     GRVLGYYVVSNDVHEQKMVEEALRRTNSILSAHFENTPLAVMEWDPSLRVIRWSGAAQ
                     AIFGWSADEVLGRSAEGWRLIYEDDAPAVGDMIRRLAEGPGSQATMLNRNYRKDGSVI
                     WVEWHNSAMRDASGHLVSILSLAQDVSSRIQAEERLQFMATHDGLTGLPNRLLLPDRL
                     ASAITRAQRFGHGVAVLFLDLDHFKDVNDTLGHRVGDELLKDLSRRIRGSLRQTDLLV
                     RLSGDEFVVVLEDLEGESGPDRVAQKILDDVMRPFPIEGHEVQVSASLGYAVFPDDGG
                     DPETLLKNADAAMYHAKELGRNSYRAFSRSLAQRRDQRFGLETALKRAIRGEEFELFY
                     QPILDVATGNVSRAEALVRWNDPARGVVLPLTFIPVAEEAGLMRGLGHWVFETAARQA
                     AAWNGAGIGPFTVSVNLSASQLRDSTIVAELEAMLLKTGCSPTWLMLEITETSMVKDV
                     EGVSLTLRKLRRMGLRIAIDDFGTGYSSLSHLRHMPVDTLKIDKSFVADIDDGTRRAA
                     ESGGAAIVAAVIGLARGLGLEVVAEGVERQAQLAFLREQGCGAYQGYLACPPLPAAEF
                     ARWLAGQPRK"
BASE COUNT          850 a         1962 c         1977 g          887 t
ORIGIN      
        1 gcttcgcctc atatgcacca cccccttgaa agtgattcag aataacgggg tgttgcatcg
       61 acccattgaa tccgccccga atgaacgtgc ttccgactgc gactccggga acatttcgac
      121 ataggttcgg acgacgacgc ccggggcaaa ttgactcggc caaaaacgaa aaaaaacacc
      181 tgccacactc ggctcctgca tcgacgaatc gcaatcgccg cttactgccc acgacgtgcc
      241 gacggggtcc gtgcagaact gactcgcaag tgccatgtac tgaccactcc atgcatcctt
      301 ccgatgccat gcctgcttac cgacgcgcct gaccttttgc ccgcatgctg aagctcttcc
      361 tgcgcctcta cctgttcctg atgctgccgg cgacggcggc gttcgtgggg ttcatgtacc
      421 tcacggacca ggtgatggcg caggttcacg ccgagacgca gcgcagccgc gcgggggcgg
      481 cattcgaccg tgccgagcgc atcatcggcg cgacggatgc cccggaccgg gaggagcggc
      541 tcaaggagat cgaggctacc ttccgcatcg agcatcgcat cctgccgatg gcgcaggtgg
      601 cggacgactg gttcatgtcg cgcccggaga aggagcgcct cgccgcgggc gggatcgcct
      661 tccgcgaccg gcccgggggc gggcaggtct acctgcgcaa gctccccggc ggggaccgcg
      721 tgctgcgcat cgagtgggtc gggctctacg agtacctggc gctctactac acgatcatcg
      781 tggcgctggt gacgctgggc ctcgccgtga tcctgctgcg ctggatgcgg cccctctacc
      841 gcgacctgga gagcctcacc gcggccgccg cgcgcgtggg cgacggcgac ctcggggcgc
      901 gggcggccgt ggggcgggcc tcgctcgtcg agcccatcgc cggggcgttc aacgcgatga
      961 ccgagcgcgt gcagcgcctg ctcgagtcgc accgcgacct ggagcagggc gtggcccacg
     1021 aactgcgcac gccgctcgcg caactcaagt tcgatctcga actcgcgcgg gagtcggaca
     1081 ccgcggcgga acgcgaggcg cgcttcgaag ccatggggcg ggacgtcgcg gacctggagg
     1141 agctggtgaa cgagctcctg cttctcgcca ggctgcgcga ggcgccgccc tacgtgcccc
     1201 gggagatccc ggcgcgcgtg ctggtggagg acgtgatccg ccgcgccagg gacgaaatgc
     1261 gcgccgccgg ccgcgaaatc gccatcgacc cgccggcggc actgccggag acgctcatct
     1321 gcgacggcaa gttcctcgcg cgcgcgctgg tgaacgtggt acgcaatgcc gtgcgccacg
     1381 ccaccgcgcg cgtggccctg tcggtcgagc gcgaaggcgg ccgcacggtg gtgcacgtgg
     1441 acgatgacgg cccgggcatc gcgccggagg accgcgagcg gctcttcgag cccttcaccc
     1501 gtgccggagc ggggcgcgaa cgcgagaccg gcggcgtggg cctcgggctc gccatcgtga
     1561 agagcgtcgc cgagtggcac ggcgggggcg cgcgcatcgg gccctcgccg ctcggcgggg
     1621 cgcgcgtgtc tatttcctgg tagcgaaaat cggggacagt ccctattttc ggggctgccc
     1681 ggcgagccag cgcgcgaact ccgcggccgg cagcgggggg caggcgaggt agccctggta
     1741 ggctccgcag ccctgctcgc gcaggaaggc gagttgcgcc tgccgctcta ccccttcggc
     1801 caccacctcc agccccagcc cgcgcgcgag cccgatcacc gcggcgacga tggccgcgcc
     1861 cccgctctcg gccgcgcgcc gcgtgccatc gtcgatgtcg gcgacgaagg acttgtcgat
     1921 cttgagcgtg tccacgggca tgtggcgcag gtgcgaaagg ctcgagtagc cggtgccgaa
     1981 gtcgtcgatg gcgatgcgca atcccatccg ccggagcttg cgcagcgtga ggctcacgcc
     2041 ctccacgtcc ttcaccatgc tggtctcggt gatctccagc atgagccagg tgggggagca
     2101 ccccgtcttc aggagcatcg cttccagttc cgcgacaatg gtggaatcgc gaagctggct
     2161 ggcggacagg ttcacgctca ccgtgaaggg gccgatgccg gcgccgttcc acgcggcggc
     2221 ctggcgcgcc gcagtctcga acacccagtg cccgaggccg cgcatgaggc cggcctcctc
     2281 cgccaccggg atgaaggtga gcggcagcac cacgccgcgc gcggggtcgt tccagcgaac
     2341 cagcgcctcc gcgcgggaca cgttgccggt cgccacgtcg aggatcggct ggtagaagag
     2401 ctcgaattcc tcgccgcgga tggcacgctt gagcgccgtt tcgaggccga agcgctggtc
     2461 gcggcgctgc gccagcgagc gggagaaggc gcggtagctg ttccggccca gctccttcgc
     2521 gtggtacatg gcggcgtcgg cgttcttgag cagcgtttcc gggtcgcccc cgtcatcggg
     2581 gaagacggcg tagcccaggc tcgcgctcac ctggacctcg tgcccctcga tggggaacgg
     2641 gcgcatcacg tcgtccagga tcttctgcgc gacgcggtcg ggtccgctct cgccctcgag
     2701 gtcctccagc accacgacga actcgtcgcc cgaaagtcgc accaggaggt cggtctggcg
     2761 cagcgaaccg cggatgcggc gcgagaggtc cttcaggagt tcgtcgccca cgcggtggcc
     2821 gagcgtgtcg ttcacgtcct tgaagtggtc gaggtccagg aacagcaccg ccacgccatg
     2881 gccgaagcgc tgggcgcgcg tgatcgccga ggcgaggcgg tcgggcagga gcaggcggtt
     2941 gggcagcccg gtgaggccgt cgtgcgtggc catgaactgc agccgttcct cggcctggat
     3001 gcgcgacgac acgtcctggg caagcgacag gatcgacacc aggtgaccgc tcgcgtcgcg
     3061 catcgccgag ttgtgccact cgacccagat caccgagccg tccttgcgat agttccggtt
     3121 cagcatcgtg gcctgcgacc cggggccttc cgcgaggcgc cggatcatgt cgcccacggc
     3181 gggcgcgtcg tcctcgtaga tgaggcgcca gccctcggcc gagcggccga gcacttcgtc
     3241 ggcgctccag ccgaagatgg cctgcgccgc ccccgaccag cggatcaccc tgagggaagg
     3301 gtcccactcc atcacggcga gcggggtgtt ctcgaaatgc gccgacagga tcgagttggt
     3361 gcggcgcagc gcctcctcca ccatcttctg ctcgtggacg tcgttggaca cgacgtagta
     3421 gcccagcacg cggccggctg cgtccttgcg cgggctgagc ttcacgcgca cccaccgttc
     3481 ctcgccatcg ccggcgacgc gcatgcgccg ctcgtaggtc accgtctcgc cggtcaccac
     3541 gcggtcgaga tagggctgca acgcctgcac gaggtcgggc gggtagacat ccttcgggaa
     3601 cttgccgagc gcgaattcac gcgtgagccc gcgcgattcc aggaacacgt tgttcacgaa
     3661 ggtgcagccg cgctcgaggt ccacgtacac gatggcctcg gggatgttct cggcgaagaa
     3721 gcgaagttcc tcctcgcggc cgcgcagttg ctcctgcgcg tccttgagct cctgcacgtc
     3781 gtggccgacc acgaacatgc cgcgcacctt gccgtcgggg ccgtggtcgg gcaccgagcg
     3841 gccgagaatc cagcgccgct cgccggaggg caggatcatc ttccgctcgt aggtcgcggt
     3901 ttgccccgac agcaccctgc tcaccatggg cgtgagtgcc gcggcggctt cctcgccgag
     3961 gacctccgcg gacgtgcgcc cgatgatgtc ctcgagcttc atgccgcgcg tgtcggcgaa
     4021 cgtgcggttg acgaacacgt agcgccgctg cgtgtccagg taggcgaccg acacggggat
     4081 gttgtcggtg taaaggcgca gcttcgcttc ctggtcggcg aggcggtcct gggccagctt
     4141 cacatcgtgg acgtccgtgc cgacgatgta gtagccgcgg aaggcgccgt cgtctcccat
     4201 gtagggctcc aggcgcatcc ggaaccagcg cttgttcccg tccccgcgtc ccagcagccg
     4261 ctcgtaggtg caggtttcgc cacccttgag gcgctcgacg aagggcgtga agtatttcgc
     4321 ggccgcctcc tcgcccagca cctcgggaac cggccgcccg atgatgtcgg ccgcggccag
     4381 cccgcgcaat cgctcgaagg tcttgttcac gaactggtag cggaagccgg catcgacgaa
     4441 cgcgacgatc tcggggatgt tctccgcgta gaagcgcaac tggcgctcct ggcgctccag
     4501 ggcgtcgcgc aggcgcctgt cggcgtcgat gtcgatcacc accacgaaga cgccgtggac
     4561 ctcccctgcc acaaggtccg gaatgaggtt cacgcgcgcc cagcggcgag tgccgtcggg
     4621 cgcggtgccc tggcgctcgt aggcgacctt ctcgccggcc atcgccttgc ccagcggctc
     4681 gagcacgacc gcgaggacct ctggcggata cacctcctcg aggctcatgc cgatgatgtt
     4741 gtcggtgtcg acgccgggca cccagtccag tcccggggtg ttggcgaacc ggaagcgccg
     4801 gtccgtcccg atataggcca tggggacgcc cacgttttcc gtgatgagct ggagttcgcg
     4861 cgtgcgggcg agcagcgatt cctggaggcg gtggtcctcc tcgatgtcca tcagcacgat
     4921 gatcgcgccg cgcaccgcgc cggattcgtc cttgtccggg atcatgtggc cacggatgcg
     4981 gcgtttctcc cggccggtcc agagcgcttc gcgctcgtag gtgaccgatt ccccggccca
     5041 cgcgcgctgc agcaacggca ggctctggga catgatctcc gggggcgcca gttgcgccat
     5101 cggccggccg accatgtccg cgggcctcag cccggcccac gcggcgctct gcgcgttggc
     5161 gaagcgcacg atgccgccgc tgtcgatgta ggtgacggga aagccgatgg tgtcggtgat
     5221 gagggagagc tggcgctcgc gcgcggcgat ggccgactgc gcctccttca ggtgctggat
     5281 gtccacgagc gcgattgcca cgccgacgac cgccccgccg ctgcgcaccg gcacgtaatg
     5341 ggcgcgcacc cagcggccct cgttggggcc ctcgagcgtc tcgcggtcga aggtgagcga
     5401 ctcccccgcc agcgcccgcg cgagcacctt gcgccggtcg tccgtgggtt cgcgcgagaa
     5461 cgccgacgac acggtgaagc cgagcatctc gccggccgtg cggccgaaat acggcggata
     5521 gcccgggttc acgtaccggt agcgcagctc cgtatcgacc acgcagcagg gcaggccgag
     5581 caggtcgaga cccggccgta gctcggccag cacggttgcc agcgcgtcgg gggggatgat
     5641 ggaaagggag ggcacggatc gcgttcggtt tgcgcg
//
LOCUS       DASUEV010000289         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_891301, whole genome shotgun sequence.
ACCESSION   DASUEV010000289 DASUEV010000000
VERSION     DASUEV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891301"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFV55_09240"
     CDS             <1..436
                     /locus_tag="VFV55_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4944527.1"
                     /translation="REVTQATGQGRAILVCHSMGGLAARAYLRAHGADRVAGIVTLGS
                     PHHGTVLAVLGAGRNARQMRIGSDFLRDLERSEGEGGPGCAAVSVYTVHDNLVAPQDS
                     SRLAWARNVSLHGVGHLAMLLDARVHQAVVDEVARLRARPGT"
     gene            complement(433..903)
                     /locus_tag="VFV55_09245"
     CDS             complement(433..903)
                     /locus_tag="VFV55_09245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX4944528.1"
                     /translation="MAIALKTGDPAPDFEGVTTDGTRVALKDFRGRKLVMYFYPMDDT
                     PGCTKQACSLRDRNADIVARGAAILGVSTQDEASHRKFTAKYRLNFPLLADKGGHVGR
                     AYGTIGGAGLLSAAKALVGMADRVTFIIDGNGRIAHVIDRPDVSSHAEEVLALL"
     gene            complement(910..2901)
                     /locus_tag="VFV55_09250"
     CDS             complement(910..2901)
                     /locus_tag="VFV55_09250"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4944529.1"
                     /translation="MSYFVTGGTGFIGRFLIDRLLQREGEIYVLVRKNSVRKLDALRP
                     RWGVHEKRVVAVLGDLGKPKLGVSAADLAKMKGRVTHCFHLAALYDLSADAESQEKAN
                     IEGTRHAVEFAGAVRAGCFHHVSSIAAAGLYDGVFREDMFDEAEELDHPYFRTKHDSE
                     GIVRKECGRPWRIYRPAIVVGHSKTGEIDKIDGPYYFFKFIQKMRKALPQWMPTVGIE
                     GGRINIVPVDFVVDAMDHIAHRKGLDGGCFHLTDPEPRRIGEILNIFARAAHAPQMTM
                     RLNAKMFGFIPDFVIDSAMSLAPVRRIQKQVLADLGIPKDMFKFINYPTRFDNRDCAR
                     ALKGSGISVPDIEDYAWRLWDYWERHLDPDLFIDHTLAGKVKDKVIVITGGTSGIGEA
                     TAYKMAEAGAQVVVVARDPEKAAPVMERIRSIGGKGRFISCDLAELADCDRLVATVLK
                     EFGRCDYLVNNAGRSIRRGIASSFERFHDFERTMQLNYFGSLRLIMGFLPAMMKQNRG
                     HVINVSSIGVLTQAPRFSAYVASKSALDSFTGCAASEFADNNIAFTTINMPLVKTPMI
                     APTRIYNHVPTLTPEQAADLVVEAIVYKPVRIATRLGIFGALLHAVAPKATQIILNTA
                     FRMFPDSTAAQGRKDGAAKEVELSPEQVAFAQITQGIHW"
     gene            3113..3553
                     /locus_tag="VFV55_09255"
     CDS             3113..3553
                     /locus_tag="VFV55_09255"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX4944530.1"
                     /translation="MARTTRTRKARSPAVTPAKAARTIRASAQKAFEAGVQAVSGVRQ
                     SAVGAFDALVKQGAALESRSRRSALAKAGKARDAACARAEEARAKTVEAVSHLERVFE
                     KRVSNTISKLGVPTARDVRALSRQVAELQVSVDRLHRSRARAAR"
BASE COUNT          549 a         1183 c         1241 g          603 t
ORIGIN      
        1 gcgcgaggtg acgcaggcga ccggtcaggg ccgggccatc ctcgtttgcc acagcatggg
       61 agggctcgcg gcgcgcgcct accttcgcgc ccacggtgca gatcgcgtcg ccggcattgt
      121 cacgctgggc agcccgcacc acggcaccgt cctcgcggtg ctgggagccg gccgcaatgc
      181 gcggcagatg cgcatcggaa gcgacttcct gcgcgacctg gagcgatcgg aaggcgaggg
      241 cggcccgggt tgcgcggccg tttcggtcta cacggtgcac gacaacctgg tggccccgca
      301 ggactcgagc cggctcgcgt gggcgcgcaa cgtctccctg catggcgtcg ggcacctggc
      361 gatgctcctc gacgcccgcg ttcaccaggc cgtcgtggac gaagtggcgc ggctgcgcgc
      421 gcggcccggg acctagagga gcgccaggac ttcctcggcg tggctggaca cgtcgggccg
      481 gtcgatcacg tgcgcgatgc gcccgttccc gtcgatgatg aaggtcacgc ggtcggccat
      541 cccgaccagc gccttggcgg ccgacaggag ccccgccccg ccgatcgttc cgtaggcgcg
      601 ccccacgtgt cctcccttgt cggccagcag gggaaagttg aggcggtact tggcggtgaa
      661 tttccggtgg gaggcctcgt cctgggtcga cacgccgagg atcgccgcgc ccctggcgac
      721 gatatcggcg ttgcggtcgc gaagcgagca cgcctgcttc gtgcagccgg gggtgtcgtc
      781 catgggatag aagtacatca cgagcttgcg gccgcggaag tccttcagcg cgacgcgggt
      841 gccgtcggtg gtgactccct cgaagtcggg ggccgggtct ccggtcttga gcgcaatggc
      901 catctgcacc taccagtgga tgccctgcgt gatctgcgcg aaggcgacct gttccggtga
      961 aagctcgact tccttcgcgg cgccgtcctt ccggccctgc gccgccgtgg agtcggggaa
     1021 catgcggaac gccgtgttga ggatgatctg cgtcgccttg ggcgccaccg cgtgcaggag
     1081 cgccccgaag atgcccaggc gggtggcgat gcgcaccggc ttgtagacga tggcctcgac
     1141 gacgaggtcc gccgcctgct ccggcgtgag cgtgggcacg tggttgtaga tccgggtggg
     1201 cgcgatcatc ggcgtcttca ccagcggcat gttgatggtg gtgaaggcga tgttgttgtc
     1261 ggcgaattcg ctcgcggcgc agccggtgaa ggaatcgagg gccgatttcg atgcgacata
     1321 ggccgagaag cgcggcgcct gcgtgagcac cccgatcgag gagacgttga tcacgtggcc
     1381 gcggttctgc ttcatcatgg ccgggaggaa ccccatgatg aggcgcaggc ttccgaagta
     1441 gttgagctgc atcgtgcgct cgaagtcgtg gaagcgctcg aacgacgagg cgatgccgcg
     1501 ccggatggac cggccggcgt tgttcacgag gtagtcgcag cggccgaact ccttgagcac
     1561 ggtggccacg agccggtcgc agtcggcgag ctcggccagg tcgcaggaga tgaacctgcc
     1621 cttgccgccg atcgacctga tcctttccat gaccggtgcc gccttctccg ggtcgcgcgc
     1681 caccaccacc acctgcgcgc cggcttccgc catcttgtag gccgtggcct ccccgatgcc
     1741 cgacgttccg ccggtgatca cgatcacctt gtccttcacc ttgccggcca gcgtgtgatc
     1801 gatgaagagg tcggggtcga ggtggcgttc ccagtagtcc cacaggcgcc acgcgtaatc
     1861 ctcgatgtcg ggcaccgaga tgccggaacc cttgagcgcc ctggcgcagt cgcggttgtc
     1921 gaagcgcgtc gggtaattga tgaacttgaa catgtccttc gggatgccga gatccgcgag
     1981 gacctgcttc tggatgcgcc gcaccggcgc gagggacatc gcggagtcga tcacgaagtc
     2041 cgggatgaat ccgaacatct tggcgttgag gcgcatcgtc atctgcgggg catgcgccgc
     2101 cctggcgaag atgttcagga tctcgccgat gcggcgcggc tccgggtcgg tgaggtggaa
     2161 acagccgccg tccaggccct tcctgtgcgc gatgtggtcc atcgcgtcca cgacgaaatc
     2221 gaccggcacg atgttgatgc gcccgccctc gatgcccacc gtcggcatcc actgcggcag
     2281 cgccttcctc atcttctgga tgaacttgaa gaagtagtac gggccgtcga tcttgtcgat
     2341 ctcgccggtc ttcgagtggc cgacgacgat cgccggacgg tagatgcgcc agggccgccc
     2401 gcactccttg cgcacgatcc cctcggaatc gtgcttggtg cggaaatagg ggtggtcgag
     2461 ctcctcggcc tcgtcgaaca tgtcctcgcg aaagacgccg tcgtagaggc ccgcggccgc
     2521 gatcgagctc acgtggtgga agcagccggc ccggacggcc ccggcgaatt cgacggcgtg
     2581 gcgcgtgcct tcgatgttgg ccttctcctg gctttcggca tcggcggaga ggtcatagag
     2641 ggccgccagg tggaagcagt gcgtcacccg gcccttcatc ttcgccaggt cggccgccga
     2701 aacaccaagc ttcggcttgc ccaggtcgcc gaggacggcg acgacgcgct tttcgtgaac
     2761 gccccagcgc gggcgaagcg catcgagctt cctcaccgag ttcttgcgca cgagcacgta
     2821 gatctcgcct tcgcgctgga ggagccggtc gatgaggaag cggccgatga agccggtgcc
     2881 gccggtcacg aaataggaca tgggttgctc ctcgggaagc gcggggttcc ggccgaatga
     2941 tacacgcgca cctccgcgga acagccggcc ctttccggca gcggaaattt agtgtgcctc
     3001 ctctaattct ttcgcctagc cttgatcctg acaccggggg cactttcgcc gccgggcgtc
     3061 gcttccccga aggagcggcg atggatcgca acccatgacg aaaggacgaa aaatggcccg
     3121 caccactcgc acccgcaagg cccgcagtcc cgcggttact cccgccaagg cggcccgtac
     3181 gatccgtgcc tccgcgcaaa aggcgttcga ggccggcgtc caggccgtct ccggcgtccg
     3241 ccagtcggcg gtcggcgcgt tcgacgcgct cgtcaagcag ggcgctgccc tcgagtcgcg
     3301 cagccgtcgt tcggcccttg ccaaggccgg caaggcccgt gacgcggcct gcgcccgcgc
     3361 cgaggaagcc agggcgaaga ccgtcgaggc cgtctcccac ctggagaggg tcttcgagaa
     3421 gcgcgtttcg aacacgatat cgaaacttgg cgttccgacg gcgcgcgatg tccgcgccct
     3481 gtcgcgccag gtggccgagc tgcaggtgag cgtcgaccgg ctgcaccggt cgcgcgcgcg
     3541 cgcggcccgg taggcaatgc ggcgcggccc gtcacc
//
LOCUS       DASUEV010000290         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1580866, whole genome shotgun sequence.
ACCESSION   DASUEV010000290 DASUEV010000000
VERSION     DASUEV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580866"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFV55_09260"
     CDS             complement(<1..279)
                     /locus_tag="VFV55_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX4944531.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTLVLSKKYGGEV
                     RAYDQIDNAPEEKARGITINTAHVEYETKNRHYAHVDCPGHADYVKNMI"
     gene            complement(358..432)
                     /locus_tag="VFV55_09265"
     tRNA            complement(358..432)
                     /locus_tag="VFV55_09265"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Thr,seq:ggt)
     gene            complement(471..544)
                     /locus_tag="VFV55_09270"
     tRNA            complement(471..544)
                     /locus_tag="VFV55_09270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(510..512),aa:Gly,seq:tcc)
     gene            complement(604..690)
                     /locus_tag="VFV55_09275"
     tRNA            complement(604..690)
                     /locus_tag="VFV55_09275"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(654..656),aa:Tyr,seq:gta)
     gene            complement(779..854)
                     /locus_tag="VFV55_09280"
     tRNA            complement(779..854)
                     /locus_tag="VFV55_09280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Thr,seq:tgt)
     gene            complement(866..1285)
                     /locus_tag="VFV55_09285"
     CDS             complement(866..1285)
                     /locus_tag="VFV55_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEX4944532.1"
                     /translation="MSEVSTKPYFIRAIYEWCSDCGFTPYLSVRVDERTRVPSEYVKN
                     GEIVLNVGLNATRNLTINNELVQFSARFSGVSREVTIPVDRVQGIFARENGQGAFFTV
                     DAPAAAVASTAAMPGAAADSEPPKPPAAGKGKLKLVK"
     gene            complement(1297..1896)
                     /locus_tag="VFV55_09290"
     CDS             complement(1297..1896)
                     /locus_tag="VFV55_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4944533.1"
                     /translation="MMTLYSGITDPFSHRCRIVLYEKGMDFEIIDVDLMNKPEDLAVM
                     NPYNTTPVLVERDLVLYESNIINEYIDERFPHPQLMPADPVMRARARLFLFRFEQELF
                     IHVKALESGPTKGHEKARLYIRENLVQIAPIFVKQKFMLGDEFSMLDVAIAPLLWRLD
                     HYQVQLNKQSSPLLKYAERLFTRQAFIDSMTASEKAMRR"
     gene            complement(2020..2724)
                     /locus_tag="VFV55_09295"
     CDS             complement(2020..2724)
                     /locus_tag="VFV55_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX4944534.1"
                     /translation="MKKLLAILLLAPGLGFAASGGVALDRAPVNVHDKLSLQRGAQIF
                     VNHCLNCHSASAMRYSRLTDLDLNEGQIRENLMFAANKIGDPMSTTLDPADGKAWFGV
                     MPPDLSLVARSRGADWLYTYLRGFYRDPATKSGWNNTVFPNVGMPHVLWEYQGSQILQ
                     ASDKEGAKLVLGDPGKLRPAEYDKYVGDLVNYLVYMGEPAQAKRVQIGIVVLFFLAIF
                     FVITVLLKKEYWKDVR"
     gene            complement(2727..>3363)
                     /locus_tag="VFV55_09300"
     CDS             complement(2727..>3363)
                     /locus_tag="VFV55_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX4944535.1"
                     /translation="SNNPDGIEIYENLDPKTGTPVDGIPFHPYYTVKDLVGVAVFLIV
                     FSAIVFFSPEVGGYFLEYNNFLPADPLTTPAHIAPVWYFTPFYAMLRAVPSFFNMQVW
                     GVIVMGAAVMIFFALPWLDRGRVKSIRYRGPIYRSFFAVFIVCFLILGYLGVEPTNVW
                     GEFAKGMPVVGGDYIATWVARVLTAVYFAFFLLMPWYTARDREKPVPQRLT"
BASE COUNT          594 a         1049 c         1076 g          644 t
ORIGIN      
        1 gatcatgttc ttcacgtagt cggcatgccc cggacagtcc acgtgcgcgt agtgccggtt
       61 cttcgtctcg tactccacgt gcgccgtgtt gatcgtgatc ccgcgcgcct tctcctccgg
      121 cgcgttgtcg atctggtcgt atgccctcac ctcgccaccg tacttcttcg acagcaccag
      181 cgtcatcgcc gccgtcagcg tcgtcttccc gtggtccacg tggccgatcg tccccacgtt
      241 cacgtgcggc ttcgtccgct caaacttgcc ctttgccatg gtcgtctctt tctgtacaga
      301 gccttggatg agaatgccca gcgtcgccgc tggccgtgcc tggtgattcg cgatgcctgg
      361 tgcccatgat gcggattgaa cgcatgacct ctcccttacc aagggagtgc tctaccactg
      421 agctacatgg gctcgatgct gctcccggcc gtttccggcg cgggcattcg tggagcgggt
      481 gaagggaatc gaaccctcgt cgtaagcttg gaaggcttct gctctaccat tgagctacac
      541 ccgcgtcgcc tgcgacccct tcgaccgtgc gcgcctcccc ggggaaacgt gcggaaaaac
      601 tgctggtgga ggaggttgga ttcgaaccaa cgtaggcgca aggccaacag atttacagtc
      661 tgcccccttt aaccactcgg gcacccctcc gggaaactct caattatcca gtctttgcag
      721 agttaggtca aataaaacaa ggttttccgt tggcgcccgc gacgggaagg gaaatcggtg
      781 gtgccggcac cctgaatcga acaggggacc taccgcttac aaggcggttg ctctaccagc
      841 tgagctatgc cggcatggcg agcgctcact tgaccagctt aagctttccc tttccggccg
      901 ccggcggctt gggcggctcc gaatcggccg cagcgcccgg catggctgcc gtcgatgcca
      961 ccgcggctgc cggcgcgtcg accgtgaaga atgctccctg gccgttctcg cgggcgaaga
     1021 ttccctgcac ccggtcgacg gggatggtca cttcccgcga aaccccgctg aatcgtgcgg
     1081 aaaactgcac cagttcgttg ttgatggtga ggttgcgcgt ggcgttgagg cccacgttga
     1141 gcacgatctc cccgttcttc acgtactccg acggaacccg ggtccgctcg tccaccctca
     1201 cgctcaggta gggcgtgaag ccgcagtccg agcaccactc gtagatggcg cggatgaaat
     1261 acggcttggt ggatacctcg gacatggggc gggccttcag cggcgcatgg ccttctcgga
     1321 agcggtcatc gagtcgatga acgcctggcg cgtgaagagc cgttcggcgt atttcaggag
     1381 cggcgacgac tgcttgttca gctggacctg gtagtggtcg agcctccaga gcagcggggc
     1441 gatcgcgacg tcgagcatcg agaattcgtc gcccagcatg aatttctgct tcacgaagat
     1501 cggggcgatc tggacgaggt tctcgcggat gtagagccgc gccttttcgt gccccttggt
     1561 gggcccggac tcgagcgcct tcacgtggat gaagagctcc tgctcgaagc ggaagaggaa
     1621 gagccgcgcc cgcgcgcgca tcaccgggtc cgcgggcatc agctgcgggt gcgggaagcg
     1681 ctcgtcgatg tactcgttga tgatgttcga ctcgtacagg acgagatcgc gctccaccag
     1741 gaccggcgtg gtgttgtagg ggttcatcac cgcgaggtcc tcgggcttgt tcatgaggtc
     1801 cacgtcgatg atctcgaagt ccatcccctt ctcgtacagc acgatccggc agcggtggct
     1861 gaacggatcg gtgatccccg aataaagcgt catcatgcct tgttcacctc ttttggattt
     1921 gcccggccgc cggggtccgc atcgcgcctc cccatgaagc gacaagcccg atgcggccct
     1981 tccgggtcgc atcgggcgaa ttccgcaccg cccgcggtac tagcgaacgt ccttccagta
     2041 ctccttcttc agcaggaccg ttatcacgaa gaaaatggcc aggaagaaga ggaccacgat
     2101 gccgatctgg acgcgcttgg cttgcgccgg ctcgcccatg tacacgagat aattcaccag
     2161 gtccccgacg tacttgtcgt attcggcggg ccggagcttg ccgggatcgc cgaggaccag
     2221 tttcgccccc tccttgtcgg acgcctgcag gatctggctg ccctggtatt cccaaagcac
     2281 gtggggcatg ccgacgttcg ggaataccgt gttgttccac ccggacttgg tcgccgggtc
     2341 gcggtagaaa ccgcgcaggt aggtgtagag ccagtccgcg ccgcgggacc gcgcgacgag
     2401 cgacaggtcc ggcggcatca cgccgaacca cgccttgccg tcggccgggt cgagcgtcgt
     2461 ggacatgggg tcgccgatct tgttcgcggc gaacatcagg ttctcgcgga tctgcccctc
     2521 gttcaggtcc aggtcggtga gcctcgaata gcgcatggcc gacgcggaat ggcagttcag
     2581 gcagtggttc acgaagatct gcgcgccgcg ctgcagcgac agcttgtcgt gcacgttgac
     2641 cggcgcgcgg tcgagcgcga cgccgccact cgcggcaaag ccgaggcccg gcgccaggag
     2701 gaggatggct agcagctttt tcatcgtcag gtcagcctct gcggcaccgg tttttccctg
     2761 tccctggccg tgtaccaggg catgagcagg aagaaggcga aatagacggc ggtgagcacg
     2821 cgcgccaccc aggtggcgat gtagtcgccg cccacgacgg gcatgccctt cgcgaattcg
     2881 ccccagacgt tcgtcggctc cacgccgagg tagccgagga tgaggaagca cacgatgaaa
     2941 accgcgaaga acgaccggta gatcggaccg cggtagcgga tcgacttgac ccggccgcgg
     3001 tcgagccagg gcagggcgaa gaagatcatc accgccgcgc ccatcacgat cacgccccag
     3061 acctgcatgt tgaagaacga cgggacggcg cgcagcatcg cgtagaaggg cgtgaagtac
     3121 cagaccgggg cgatgtgcgc gggggtggtg agcgggtcgg cagggaggaa gttgttgtac
     3181 tcgaggaagt agccccccac ctcgggcgag aagaacacga ttgcggagaa cacgatcagg
     3241 aagaccgcca cgcccacgag gtccttgacc gtgtagtagg gatggaaggg gattccgtcc
     3301 acgggcgtgc ccgtcttcgg gtccaggttc tcgtagatct cgatgccgtc cgggttgttc
     3361 gag
//
LOCUS       DASUEV010000291         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_892711, whole genome shotgun sequence.
ACCESSION   DASUEV010000291 DASUEV010000000
VERSION     DASUEV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892711"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFV55_09305"
     CDS             <1..568
                     /locus_tag="VFV55_09305"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX4944536.1"
                     /translation="SKSLGNVFTAREVLATLDPVQGGEQLRFFLLRGHYRSELHYSAD
                     LLDDAGNTLRGFYTALREVPAQVDAIDWSHPLATRFRDAMNDDFDTPVAFAVLHELRG
                     EVNRTRSAPLAGLLKALGATIGFLQHDPEAFLQGGVTLDVDALVAQRAAAKKSRDFAR
                     ADAIRAELDAAGIVLEDKPGGATEWRKK"
     gene            complement(621..1742)
                     /locus_tag="VFV55_09310"
     CDS             complement(621..1742)
                     /locus_tag="VFV55_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944537.1"
                     /translation="MLRALLSLLALFVPGTVAFAQGHTDTQIVLGQSVALTGPAEELG
                     KDMQLGASLYFSQVNAKGGVNGRRIVLKTLDDGYEPPRAADNTKKLINEEKVFALFGY
                     VGTPTAQASLPIFTAAKVPFVGAFTGAELLRSPFNRYVFNVRASYFDETEAIIQHLTA
                     MSVNRIAVFYQNDAYGQAGLAGVERALKKRSLELVTKGTVERNTVDVAKSVADIRKAN
                     PQAVVMISAYKSCAAFIKEMKKAGANPTFWNVSFVGSKALAKELDREGRGVQISQVVP
                     FPWDTSVPVVKEYRALLAETKVEPGYGTLEGFIAAKVMVEGLRRAGKNLTRDSFIKAM
                     ESLDPYDTGGFKVSYSGDNHNGSRFVDLTIISRDQKFVR"
     gene            complement(1839..2969)
                     /gene="dnaJ"
                     /locus_tag="VFV55_09315"
     CDS             complement(1839..2969)
                     /gene="dnaJ"
                     /locus_tag="VFV55_09315"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX4944538.1"
                     /translation="MATRKRDYYEVLGVNRDASEEDIKKAYRKLAMKHHPDRNPESKD
                     AEERFKEAKEAYEMLSDAEKRRAYDAYGHAGVNPQMGGMGGAGDAGFGGFAEAFGDIF
                     GDIFGGARARSSVYRGADLRYNLEITLEQAARGTETKIRIPVMEECETCHGSGAKPGT
                     QAKTCSTCGGTGAVRMSQGFFSVQQTCPTCHGSGKMISDPCHACSGAGRVKRHKTLAV
                     KIPPGIDEGDRIRLAGEGEAGMNGGPPGDLYVVIHLKEHSVFQRDGNDLHCQMPVSFT
                     TAALGGEIGIPTLEGQAKVKVAPETQTGQVLRLRGKGIKGVRTSYPGDLLCEIVVETP
                     VKLTDRQKELLRELEEINRHDGDRHNPRAKSFMDRVKDFFAG"
BASE COUNT          438 a         1010 c         1006 g          574 t
ORIGIN      
        1 gtcgaagtcg ctgggcaacg tgttcacggc gcgcgaggtg ctcgcgaccc tcgatcccgt
       61 ccagggcggg gagcagctgc gcttcttcct gctgcgcggc cattatcgca gcgagctcca
      121 ctattccgcg gatctcctgg acgacgccgg caatacgctg cgcggcttct acacggcgct
      181 gcgcgaggtg cctgcgcaag tcgacgccat cgactggtcg cacccgctgg ccacgcgctt
      241 ccgcgacgcg atgaacgacg atttcgacac gcccgtcgcc tttgccgtgc tgcacgagct
      301 gcgcggcgag gtgaacagga cgcgctcggc gccgcttgcg ggactgctca aggcgctcgg
      361 cgcaacgatc ggattcctgc agcatgaccc ggaggccttc ctgcagggcg gcgtgaccct
      421 cgacgtcgac gcgctggttg cgcagcgcgc ggccgcgaag aagtcgcgcg atttcgcgcg
      481 ggcggacgcg atacgtgcgg agctcgatgc ggcgggcatc gtcctggaag acaagccggg
      541 gggtgccacc gagtggcgca agaaatgaac agggcccggt gcaaccgggc cctgttccga
      601 cggggaccgc ggtaacgccg tcagcgcacg aacttctggt ccctggaaat gatcgtcagg
      661 tcgacgaatc tcgagccgtt gtggttgtcc ccgctgtagc taaccttgaa gcccccggtg
      721 tcgtagggat ccagcgactc catcgccttg atgaagctgt cgcgcgtcag gttcttgccg
      781 gcgcggcgca gcccctcgac catgacctta gcggcgatga agccctcgag cgtgccgtag
      841 cccggctcga ccttcgtttc cgcgaggagg gcgcggtatt ccttgaccac cggcaccgac
      901 gtgtcccacg gaaacgggac gacctgcgag atctggaccc cgcggccttc gcgatcgagt
      961 tccttggcga gggccttgct tcccacgaag gagacgttcc agaacgtggg gttcgccccc
     1021 gctttcttca tctccttgat gaatgcggca caggacttgt aggcgctgat catcacgacc
     1081 gcctgcgggt tcgccttgcg gatatcggcc acggacttcg cgacgtcgac ggtgttgcgc
     1141 tccacggtgc ccttggtcac cagctcgagg ctgcgcttct tgagtgcgcg ttccacgccc
     1201 gcgagccctg cctggccgta cgcgtcgttc tggtagaaga ccgcgatgcg gttcacgctc
     1261 atcgccgtca ggtgctggat gatcgcctcg gtctcgtcga agtagcttgc acggacgttg
     1321 aagacgtagc ggttgaacgg cgatcgcagc agttccgcgc cggtgaatgc gcccacgaag
     1381 gggaccttcg cggccgtgaa gatcggcagg gatgcctggg ccgtgggcgt acccacgtag
     1441 ccgaagagcg cgaagacctt ctcctcgttg atcagcttct tcgtgttatc cgcggcgcgc
     1501 gggggctcgt agccgtcgtc gagcgtcttg aggacgatgc gacggccgtt caccccgccc
     1561 ttggcgttga cctggctgaa gtacaggctc gcgccaagct gcatgtcctt gcccagttcc
     1621 tccgccgggc cggtcagcgc caccgactgg ccgaggacga tctgcgtatc ggtgtggccc
     1681 tgcgcgaatg ccaccgtgcc cgggacgaac agtgcgagta acgacagtag tgcgcgtaac
     1741 atcttttctc ccctggatgg tccgggaatt cctcggttcg tcgggaatcc gcttgcaggc
     1801 catcataacg cggcccgtcg tggcgaagaa agccggggct agccggcgaa gaagtccttc
     1861 acgcggtcca tgaaggactt ggcgcgcgga ttgtggcggt cgccgtcgtg gcggttgatc
     1921 tcctccagtt cgcgcaggag ctccttctgc cggtcggtga gcttcaccgg ggtctcgacc
     1981 acgatctcgc acagcaggtc gcccgggtag ctcgtgcgga cccccttgat tcccttgccg
     2041 cgcaggcgca gcacctgtcc ggtctgcgtc tccggggcga ccttcacctt ggcctgcccc
     2101 tcgagggtcg gaatcccgat ctcgccgccg agagccgccg tggtgaagct caccggcatc
     2161 tggcaatgca ggtcgttgcc gtcgcgctgg aagacggagt gctccttgag gtggatcacc
     2221 acgtagaggt ctcccggcgg gccgccgttc atgccggcct cgccctcgcc ggcgagccgg
     2281 atgcggtcgc cctcgtcgat gcccggcggg atcttcacgg cgagcgtctt gtggcgcttc
     2341 acgcgacccg cgccgctgca ggcatggcag gggtcggaga tcatcttgcc gctgccgtgg
     2401 caagtggggc aggtctgctg gaccgagaag aagccctgcg acatgcgcac ggcgcccgtg
     2461 ccgccgcagg tcgagcacgt ctttgcctgc gtgcccggct tggcgccgct gccgtggcag
     2521 gtttcgcact cctccatgac gggaatgcgg atcttcgtct ccgtgccgcg cgcggcctgt
     2581 tccagcgtga tctcgaggtt gtagcgcagg tccgcgccgc ggtagacgct cgagcgcgcg
     2641 cgcgcgccgc cgaagatgtc gccgaagatg tcgccgaagg cctcggcgaa gccgccgaat
     2701 cccgcgtcgc ccgccccgcc catcccgccc atttgcggat tcacgccggc gtgcccgtag
     2761 gcatcgtagg cgcgacgctt ctcggcgtcc gagagcatct cgtaggcttc cttcgcctcc
     2821 ttgaaccttt cctccgcgtc cttgctttcg ggattgcggt cggggtggtg cttcatggcg
     2881 agcttgcgat aggctttctt gatgtcctcc tcggaggcat cgcggttcac gcccagcact
     2941 tcgtagtagt cgcgcttcct ggtcgccatg gcgggttctt aacgtgtcat tcccgcgcag
     3001 gcgggaatcc agccaaaacg ctcgcgcg
//
LOCUS       DASUEV010000292         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_303686, whole genome shotgun sequence.
ACCESSION   DASUEV010000292 DASUEV010000000
VERSION     DASUEV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303686"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..656)
                     /locus_tag="VFV55_09320"
     CDS             complement(102..656)
                     /locus_tag="VFV55_09320"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX4944539.1"
                     /translation="MDIPGLIDQYGYLAVVVGAFLEGETVIALAGFAAHRGYLDFAAV
                     AALAGLAGFAGDQFYFFLGRYRGAQVLARFPGAQAKAQRVDDMLARWHAPFIVMLRFM
                     YGFRIAGPVLLGVGQCPAWKFVVFNAIGAALWAPAVAGAGWVFGEAAQRVLADIRNYE
                     GWLFAAIVLAGLAGVAIHRFRRRS"
     gene            772..1983
                     /locus_tag="VFV55_09325"
     CDS             772..1983
                     /locus_tag="VFV55_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX4944540.1"
                     /translation="MEALKTSADVLFILLGAVMVLAMHAGFAFLELGTVRRKNQVNAL
                     MKILVDFSVSTIAYFFIGYSVAYGASFFAGAESLAAKSGYELVRFFFLLTFAAAIPAI
                     VSGGIAERFRFNPQIMATFVLVGFLYPFFEGIAWNQAFGFQSALKGAFGAEFHDFAGS
                     VVVHAMGGWVALAAVLLLGARHGRYSKDGHVMAFPPSSIPFLALGAWVLSVGWFGFNV
                     MSAQTIDKVSGLVAVNSLMAMSGGTIAGLIAGRNDPGFVHNGPLAGLVAICAGSDVMH
                     PIGALATGAVAGGLFVVMFNATQNRLRIDDVLGVWPLHGLCGAWGGIACGIFGSKALG
                     GMGGVSFASQFAGTALGIAVALAGGFALYGALRATVGIRLDAEEEFNGADISIHRISA
                     TPERAESEDRT"
BASE COUNT          324 a          657 c          707 g          327 t
ORIGIN      
        1 acgacatcat cgtccgtacc ccgtcgtaac ccgccgcggg acggttccgc gtacccggta
       61 cgtgtacccg gtacatgtac cgggtacagg aaaattgcct gctaggagcg ccgtcgaaac
      121 cggtggatgg ccacgccggc aagccccgcc agcacgatcg cggcgaagag ccacccctcg
      181 taattgcgga tgtcggcgag cactcgctgc gcggcctcgc cgaataccca gccagccccg
      241 gcgactgccg gtgcccagag cgcggcgccg atggcattga agaccacgaa cttccacgcg
      301 ggacactgcc cgacgccgag cagcacgggt cccgcgatcc ggaagccgta catgaatcga
      361 agcatcacga tgaacggggc gtgccagcgc gccagcatgt cgtccacgcg ctgcgccttc
      421 gcctgcgcgc cggggaaccg cgcaagtacc tgcgcccccc ggtaacggcc gagaaagaag
      481 tagaactggt cgcccgcgaa gccggccagg cccgccagcg ccgcgacggc agcgaagtcc
      541 aggtagccgc gatgggccgc gaagccggcc agcgcgatga ccgtctcgcc ctcgaggaag
      601 gcgccgacga ccacggcgag gtaaccgtac tggtcgatga ggccggggat gtccatggaa
      661 tcgcacggag catcggccat cctccagcgc gatgcaaggg agcgggcgta tagtggcgcc
      721 acgaccgcgg cgaacgcggc gacgacagcg aatgcccggg gggagatctc gatggaagcg
      781 ctaaagacgt cggccgacgt acttttcatc ctgctcggcg ccgtcatggt gctggcgatg
      841 cacgcgggtt tcgcattcct ggagctgggc acggtccggc gcaagaacca ggtgaacgcg
      901 ctgatgaaga tcctggtgga tttctcggtc tccacgatcg cctatttctt catcggctat
      961 tcggtcgcct acggagccag cttcttcgcc ggtgcggaga gcctggccgc gaagagcggc
     1021 tatgagctgg tccgtttctt cttcctgctc accttcgccg ccgcaatccc ggccatcgtg
     1081 tcgggcggca tcgcggagcg cttccgcttc aacccgcaga tcatggcgac cttcgtgctg
     1141 gtgggcttcc tgtacccgtt cttcgagggg atcgcgtgga atcaggcgtt cggcttccag
     1201 tccgcgctga agggcgcgtt cggggcggaa ttccacgatt tcgcggggtc ggtggtggtg
     1261 cacgcgatgg gcggctgggt ggcgctcgcg gcggtgctgc tgctgggcgc ccggcacggg
     1321 cggtacagca aggacgggca cgtgatggcc ttcccgccat ccagcatccc tttcctggcg
     1381 ctgggcgcgt gggtgctctc ggtgggctgg ttcgggttca acgtgatgag cgcgcagacc
     1441 atcgacaagg tctcgggcct ggtcgcggtc aactcgctca tggcgatgag cggcgggacg
     1501 atcgcgggcc tcatcgccgg gcgcaacgat ccgggcttcg tgcacaacgg gccgctcgcg
     1561 ggactggtgg cgatttgcgc aggctcggac gtgatgcacc cgataggcgc gctcgccacg
     1621 ggagcggtgg caggcggact gttcgtcgtc atgttcaacg ccacgcagaa tcgcctacgg
     1681 atcgacgacg tgctgggcgt ctggccgctg cacgggctgt gcggcgcatg gggcggcatt
     1741 gcctgcggca tcttcggctc gaaggcgctg ggcgggatgg ggggtgtctc cttcgcgagc
     1801 caattcgccg gcacggcgct cgggatcgcg gtcgcgctcg cgggcggatt tgccctctac
     1861 ggcgcgttgc gcgccaccgt cggcattcgc ctcgacgcgg aggaggaatt caatggcgcc
     1921 gacatctcga tacacaggat cagcgccacg ccggagcgcg ccgagagcga ggaccgcacc
     1981 tgaagtccct cgtccggccc catgcggcaa tggag
//
LOCUS       DASUEV010000293         4820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_764963, whole genome shotgun sequence.
ACCESSION   DASUEV010000293 DASUEV010000000
VERSION     DASUEV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764963"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VFV55_09330"
     CDS             complement(<1..1222)
                     /locus_tag="VFV55_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4944541.1"
                     /translation="MRGFAALALVAGFSLPVAAQQGRDSPPGREVAMSEYLAAELAAQ
                     RGETDAALAAMARLARELRDPQIARRAVELAIRARAMEPALEMAMLLVGLDPESTLGR
                     DLVASLVSSRGDVDKARDSLAAFVESSPDRPFLLTQLAFFFGKYTDKAAVLEATRTIA
                     ARYPDLAESHYALGVAALVAGRQELAKSESDAALRMKPGWAQGAILQAQVLKKDTPAE
                     VIPYYRSFVERYPDSREVRLQLARELTGAKLNAEARQEFREVERLSVSDPQVPYAIGL
                     LSLQLEDYADAEAAFRRALGRGHPDPGAIYLGLGQAAEAANNLDEALDWYRKVESDDW
                     MRAQMRIASLIAKRDGLAEGRAYLQKLEPRTQDDRIQIVQMEAQLLRDAKAWQDTFDM
                     LSSAVERIPDSFELL"
     gene            1394..2206
                     /gene="mutM"
                     /locus_tag="VFV55_09335"
     CDS             1394..2206
                     /gene="mutM"
                     /locus_tag="VFV55_09335"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX4944542.1"
                     /translation="MPELPEVETTRLGLLASVVGRRIRDVVVREPRLRWPVPRDLAAR
                     LRGVEVSAIRRRGKYLLFDCGTGHLLVHLGMSGSLTVVPPGRPVRRHDHVDLALDSGD
                     IVRLNDPRRFGAVLWVRDPDRHALLAGMGAEPLDEAFGGAYLYHASRGRRIAVKQFLM
                     DARVVTGIGNIYANESLFAARIRPSRPAGRISRSRYDGLALEVRATLTRALAAGGSTL
                     RDFVGSNGEPGYFQLEYAVYGRGGEPCPACGTPVRASRHAGRSTFHCPSCQR"
     gene            2203..4155
                     /locus_tag="VFV55_09340"
     CDS             2203..4155
                     /locus_tag="VFV55_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX4944543.1"
                     /translation="MKSDGANPDRGAFEPSLVALKQWREEVAAALADLRRWAIVHRLT
                     DDHAAARLAHLERRLASERLSIAFVAEFSRGKSELINALFFADLGARLLPSGAGHATM
                     CPTEILYDPSSPIAIRLLPIETRGSPRSLREFLAEADGWKEIAIDPGRPESLATAFDA
                     LFETLSVTASQAHGLGLPDEAGPLAEIPRWRYAIVNFPNPLLAMGLTILDAPGLKALD
                     AEPELAMHRLQEADAVVFMLSAETGPTPADLDLWRRHVEPMDDLGDARYVVINRIDTL
                     RDGVRTEAQVFSEIDRQVRATADALGVDPTTVYPLSARQGLAARIGRDGGGLAKSRLY
                     RLEQALAGGLVQGRRLDQATSVRAEVRTILAEARALLDSRRAFVEEQLVELGQLQGKN
                     QKLVDSLGRRAAGERLRIDDARTAITGLRAVHNRHADDLAQLLSPEDARASGVRARAA
                     VLSTAFSAGIGEVVDGYFRENRERIARAVEVIGEVKAMMATVNRRFAESWGLSPVEVA
                     PFSTDRFLLELDRLEEQCSRNFRSASSLLTRGRSTLAALFFDTAALKAIHVFEIADRE
                     VRAWMNSFIRPLEAQVNAFQEQANSRIEGMARIQDAESGLAERIDDLRAFVRDCDERA
                     HEWKMHDERLARLLDPVAEAPFAPPR"
     gene            complement(4152..4421)
                     /locus_tag="VFV55_09345"
     CDS             complement(4152..4421)
                     /locus_tag="VFV55_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX4944544.1"
                     /translation="MALMITDACINCDVCEPECPNGAISAGPEIYVIDPRKCTECVGH
                     FDVPQCREVCPVDCIPLNPEWKESQDGLRAKYERLVASGVAKAAG"
     gene            complement(4432..>4820)
                     /gene="coaD"
                     /locus_tag="VFV55_09350"
     CDS             complement(4432..>4820)
                     /gene="coaD"
                     /locus_tag="VFV55_09350"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX4944545.1"
                     /translation="QSQAKRPFFSLDERVGLAREVLKPYANVKVEGFNGLLSEFVREH
                     QAGVILRGLRAVSDFEYEFQLAGMNRTLAPGVETLFLTPSDRYLFLSATIVREIAVLG
                     GDIAAFVHPITAERIRDKIGRTPVGE"
BASE COUNT          715 a         1745 c         1642 g          718 t
ORIGIN      
        1 agaggagctc gaacgaatcc gggatgcgct ccaccgcgct cgacagcatg tcgaacgtgt
       61 cctgccaggc cttcgcgtcg cgaagaagct gcgcctccat ctggacgatc tggatgcgat
      121 cgtcctgggt gcgcggctcg agcttctgca ggtacgcgcg gccctccgca aggccgtcgc
      181 gcttcgcgat gagagaggcg atccgcatct gcgcgcgcat ccagtcgtcc gactcgacct
      241 tgcggtacca gtcgagggcc tcgtccaggt tattggcggc ttccgccgcc tgcccgagcc
      301 cgaggtagat cgcgcccggg tccgggtggc ccctgcccaa ggcgcggcgg aacgcggctt
      361 ccgcatccgc gtaatcctcc agctgaagcg agagcaggcc gatggcgtaa ggcacctgcg
      421 ggtcggagac ggagagccgt tcgacttccc ggaactcctg cctggcctcg gcgttgagct
      481 tggcaccggt gagttcgcgc gcgagctgca ggcggacttc cctggaatcg gggtaacgct
      541 cgacgaacga gcggtagtac gggatcactt ccgcaggagt gtccttcttg aggacctggg
      601 cctggaggat ggccccctgt gcccagccgg gcttcatccg gagggcggca tcggactcgg
      661 atttcgcgag ttcctggcgg cccgcgacga gcgccgccac gccaagggcg tagtgcgact
      721 ccgcaaggtc aggatagcgc gcggcgatcg tgcgcgtggc ttccagcacc gccgccttgt
      781 ccgtgtactt gccgaagaag aacgcgagct gggtgaggag gaagggccgg tccggactgg
      841 actccacgaa ggccgcgagg gaatcgcgcg ccttgtcgac gtcgccgcgg ctcgacacca
      901 gggaagccac gaggtcgcgg ccgagggtcg actcggggtc gagcccgacc agcagcatcg
      961 ccatctccag cgccggctcc atcgcccggg cgcggatcgc gagctccacc gcgcggcgcg
     1021 cgatctgcgg gtcgcgaagc tcgcgtgcaa gccgggccat ggcggccagg gcggcatccg
     1081 tttcgcctcg ctgcgcggcc aactcggctg ccaggtactc ggacatggcg acctcgcgcc
     1141 ccgggggcga atcccgcccc tgctgcgcgg ccaccgggag cgaaaagccc gccaccaggg
     1201 ccaacgccgc aaaacctctc acgcccttca ccatcgatcg tcgctcccgc cgcaagcccc
     1261 gcgcccatcg cgccagtcaa tcatagtgct tttccgccgc ccgcccacgg cgacgcaggg
     1321 gttggaccgc gccatggcgc ggtcgtttcg gcccgcggcc ttataatcgg gcgcagattc
     1381 cctccccttt cccatgccgg aactccccga agtcgagacc acccgcctgg gtctccttgc
     1441 cagcgtcgtg gggcgccgca tccgcgacgt cgtggtgcgg gagccgcgcc tgcgctggcc
     1501 cgtgccccgc gacctcgcgg cgcgcctgcg cggcgtggaa gtgtccgcga tccgccggcg
     1561 cggcaagtac ctgctgttcg attgcgggac cggccatctt ctcgtccacc tcggcatgtc
     1621 cggcagcctc acggtggtgc cgccggggcg tcccgtgcgc cgccacgatc acgtggacct
     1681 ggccctcgac agcggcgaca tcgtgcgcct gaacgacccg cgccggttcg gggccgtgct
     1741 gtgggtacgc gatcccgaca ggcacgcgct gctggccggg atgggcgccg agcccctcga
     1801 cgaggcgttc gggggcgcat acctgtacca cgcctcgcgc gggcggcgga tcgcggtgaa
     1861 gcaattcctg atggatgctc gcgtggtcac ggggatcggc aacatctacg ccaacgagtc
     1921 gctgttcgcc gcccgcatcc gtccgtcgcg accggccggg cgcatctcgc gcagccgcta
     1981 cgacggcctt gccctcgagg ttcgtgccac gctcacccgc gcgctcgccg ccggcggcag
     2041 cacgcttcgc gatttcgtgg gaagcaacgg cgagcccggc tacttccagc tggagtacgc
     2101 ggtatatggg cgcggcggcg agccctgtcc ggcctgcggt accccggttc gcgcctcgcg
     2161 ccacgcgggc cgctcgacct tccactgccc gtcatgccag cgatgaaaag cgacggggcc
     2221 aacccggacc gcggcgcctt cgagccgagc ctggtcgcgc tgaagcagtg gcgcgaggag
     2281 gtggccgcag ccctcgcgga tcttcgccgc tgggccatcg tgcatcgcct gaccgacgac
     2341 cacgccgcgg cgcggctggc tcacctcgag cggcgcctcg cctcggaacg gctgtcgatc
     2401 gccttcgtcg cggagttctc gcgcgggaaa agcgagctca tcaacgcgct gtttttcgcc
     2461 gacctcgggg cgcggcttct gccttccggc gcagggcacg cgacgatgtg cccgaccgag
     2521 atcctttacg acccgtcgag cccgatcgcg atccggctcc tgcccatcga gacgcgcggc
     2581 agcccgcgtt cccttcgcga gttccttgcc gaggcggacg gctggaagga aatcgccatc
     2641 gacccggggc gccccgagag cctcgccacg gccttcgacg cgcttttcga aacgttgagc
     2701 gtcaccgcgt cccaggcgca cggcctcggc ctgccggacg aggcgggacc gcttgccgaa
     2761 atcccgcgct ggcgctacgc catcgtgaac ttcccgaatc cgcttctcgc catggggctc
     2821 accatcctgg acgccccggg gctcaaggcg ctcgacgccg agccggaact cgcgatgcac
     2881 cgcctccagg aagcggacgc cgtcgtcttc atgctctccg cggagaccgg cccgaccccc
     2941 gccgacctcg acctgtggcg ccggcacgtg gagccgatgg acgacctcgg cgatgcgcgc
     3001 tacgtggtga tcaacaggat cgacaccctg cgtgacggtg tcaggaccga ggcccaggtc
     3061 ttctccgaaa tcgaccggca ggtgcgcgca acggccgacg cgctgggcgt cgaccccacg
     3121 accgtctatc cgctgtcagc ccggcaaggc ctggcggccc gcatcgggcg cgacggcggc
     3181 gggctggcca agagccgcct ctaccgcctc gagcaggcgc tggcgggcgg gctcgtccag
     3241 gggcgccggc tggaccaggc cacatcggtg cgggcggaag tgcggacgat cctcgccgaa
     3301 gcacgcgccc tcctcgacag ccgccgcgca ttcgtcgagg agcagctcgt cgagctcggg
     3361 cagctgcagg gcaagaacca gaagctcgtg gattcgctgg gccgcagggc ggccggcgag
     3421 cggcttcgca tcgatgacgc gcgcaccgcg atcacgggac tgcgcgccgt ccacaaccgc
     3481 cacgccgacg atctcgcgca gctcctgagc ccggaagatg cgcgcgcgtc cggcgtgcgt
     3541 gcgcgcgccg cggtcctctc gaccgccttc agcgccggca taggcgaagt ggtcgacggc
     3601 tacttccgcg agaaccggga gcggatcgcg cgagccgtgg aggtgatcgg cgaggtgaag
     3661 gcgatgatgg ccacggtgaa ccgccggttc gccgaatcgt ggggcctctc gccggtcgag
     3721 gtggccccgt tctcgacgga ccggttcctc ctggagctcg accgcctcga ggaacagtgc
     3781 tcgcgaaact tccgcagcgc gtccagcctg ctcacgcgcg gtcgcagcac gcttgccgcc
     3841 cttttcttcg acacggccgc gctcaaggcc atccacgtct tcgagattgc cgaccgcgag
     3901 gtccgcgcct ggatgaattc gttcatccgt cccctggaag cccaggtgaa cgcgttccag
     3961 gagcaggcga acagccgcat cgaggggatg gccaggatcc aggatgccga gtctggcctg
     4021 gcggagcgca tcgacgacct gcgcgcattc gttcgcgact gcgacgagcg ggcgcacgag
     4081 tggaagatgc acgacgagcg cctcgcaagg ctgctcgacc ccgttgccga ggcgcctttc
     4141 gcgccgccgc gctagcccgc cgccttcgcc accccggaag ccacgagccg ctcgtacttt
     4201 gcccgcagcc cgtcctgcga ttccttccac tcggggttga gcggaatgca gtccaccggg
     4261 cacacctcgc ggcactgcgg cacgtcgaag tgaccgacgc actcggtgca cttgcgcggg
     4321 tcgatcacgt agatctcggg gcccgcggaa atggcgccgt tggggcactc cggctcgcag
     4381 acgtcgcaat tgatacaggc atccgtgatc atcaaggcca cggcgatccc ctcactcccc
     4441 gaccggcgta cgaccgatct tgtcgcggat gcgctcggcg gtgatcgggt ggacgaaggc
     4501 cgcgatgtcg ccgcccagga ccgcgatctc gcgcacgatc gtggccgaca ggaacaggta
     4561 gcggtccgac ggcgtgagga agagcgtctc cacgccgggc gcgagcgtgc ggttcatgcc
     4621 ggccagctgg aactcgtact cgaagtccga cacggcgcgc agcccccgca ggatcacgcc
     4681 cgcctggtgc tcgcggacga actccgagag cagcccgttg aagccctcca ccttcacgtt
     4741 cgcgtagggc ttgaggacct cgcgcgcgag gcccacgcgc tcgtcgagcg agaagaacgg
     4801 gcgcttggcc tggctctgcg
//
LOCUS       DASUEV010000294         8210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1462618, whole genome shotgun sequence.
ACCESSION   DASUEV010000294 DASUEV010000000
VERSION     DASUEV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462618"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1033
                     /gene="amrB"
                     /locus_tag="VFV55_09355"
     CDS             221..1033
                     /gene="amrB"
                     /locus_tag="VFV55_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX4944546.1"
                     /translation="MLATRPAAVAGSFYPGDPSTLAVEVAACLRDAVKPLPDERAPKA
                     IIAPHAGYVYSGPIAASAYARVAPLRGKISRVVLAGPAHRVPVAGAAVPQAGAFATPL
                     GDVPLDEEALARLRRLPFVETSDRSHALEHSLEVHLPFLQSVLGRFRLVPVVVGDVSP
                     GEMAELLDEVWGGDETLVVVSSDLSHYLPYGAARERDRATAASILALSPRLDPQDACG
                     AAPVNGLLEVARRRGLEVELLDLRNSGDTAGNRSQVVGYGAFMFREPEPHHA"
     gene            1026..1589
                     /gene="amrA"
                     /locus_tag="VFV55_09360"
     CDS             1026..1589
                     /gene="amrA"
                     /locus_tag="VFV55_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX4944547.1"
                     /translation="MLEPLDRGTVLVTIAREAIGETFGLVVPRQRDESWLARPAATFV
                     TLRLDGGLRGCIGSMEPRRPLGEDVACNARAAAFSDPRFEPLTIGEFASVCLEVSVLS
                     PRTLLVAQSESEAAVALRPGVDGVYLEYQGLAATFLPQVWEDLPDPVDFLAALRHKAR
                     LPARFWDPAIRLTRYTVEKYGDGHPAH"
     gene            1570..2682
                     /gene="amrS"
                     /locus_tag="VFV55_09365"
     CDS             1570..2682
                     /gene="amrS"
                     /locus_tag="VFV55_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX4944548.1"
                     /translation="MDTRHIDPVEGSHHPARWWHRLDDGRIQCDLCPRDCRLHEGQRG
                     ACFVRARSGDAMVLTTYGRSSGFCLDPIEKKPLNHFFPGTSVLSFGTAGCNLACKFCQ
                     NWDISKSRDMDRMMDQASPEAIARAARETGARSAAFTYNDPVIFAEYAIDVADACRAA
                     GIATVAVTNGYMHAEPRREFYARMDAANVDLKAFTDGFYVKLTGAKLAPVLDTLSYLV
                     HETKVWTEITTLLIPGHNDSAAEIEAESRWIMRELGPDVPVHFTAFHPDYKMTDLPVT
                     PAATLERARRIALGEGLRYVYTGNVHDREGGTTFCPGCHAALVERDWHRIDAYRLTAD
                     GHCPDCGTAIAGRFEAFDPKRQFGRRRIPLMLAGVA"
     gene            complement(2679..3605)
                     /gene="rsgA"
                     /locus_tag="VFV55_09370"
     CDS             complement(2679..3605)
                     /gene="rsgA"
                     /locus_tag="VFV55_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX4944549.1"
                     /translation="MTTRRERDQQVVVEGRVVADFGRDFLVELADRRQIACTRKGRKQ
                     DAACGDFVEVRLTGSAQGSIERVGTRRNILFRSDQWKEKTLAANVDQAVILLAPRPAF
                     SEAFLNLSLVACDAAGIPVIIALNKSDLPEHKAARESLVPWERIGYPVISMSAKFDTS
                     ALLPLLDGNITLLVGQSGMGKSKTVNALVMNEVARVGEISASLGIGTHTTTFSRMFRL
                     DRDAAIIDTPGFQSFGLFHLTEGQIAEAMREFRPFLGACKFNDCAHLREPGCAVIEAA
                     GKGTISAERLSFYQVLIEQHRELHDQHPDWIS"
     gene            complement(3647..3976)
                     /locus_tag="VFV55_09375"
     CDS             complement(3647..3976)
                     /locus_tag="VFV55_09375"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX4944550.1"
                     /translation="MSDLARKKCLPCEGGVAPLTEAQIGPLLKGLDGWAREGEAIAKT
                     YRFSNYHETMAFVNATAWISHREDHHPDLAVGYNQCKVSYWTHAVGGLSENDFICAAK
                     IDALLDL"
     gene            complement(4004..5275)
                     /locus_tag="VFV55_09380"
     CDS             complement(4004..5275)
                     /locus_tag="VFV55_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX4944551.1"
                     /translation="MNTFATIFVVFALAALAWRLWLMGRHARHVARHRDRVPEDFAAL
                     VPPEAHAKAADYTRDKIRVAAVEAVVVDGALLLALSVGGGLALLDGASRSLFGEGYAR
                     DLATVFGLMGVGAITSLPFDAWRTFRVEARYGFNQTTLATFAADIAKGLALAVAIGAP
                     VVLAVCWFIESTGAWWWVWAWIAWVAFSLVLVAIFPRWIAPLFNRFTPLEEGELRSRI
                     EALVARCGFRAEGLFVMDGSKRSSHGNAYFTGFGRAKRIVFFDTLMERLTQGEIEAVL
                     AHELGHFARGHIPRLLAVRFALAFGLLAVLGWAAREPAFAVALGLSSPPPTGALLGAF
                     MLVVPVFTFPFQPLASLLSRRQEFEADEYAANHASADDLVNALGKLYRDNAATLTPDP
                     LHSLVYDSHPPAALRIAQLRRLAAKSACTVA"
     gene            5372..5923
                     /gene="orn"
                     /locus_tag="VFV55_09385"
     CDS             5372..5923
                     /gene="orn"
                     /locus_tag="VFV55_09385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX4944552.1"
                     /translation="MAQEKAPLVWVDMEMSGLVPDRDRILEVAIVVTDGELATIAEAP
                     VWVIHQPDEVLDAMDSWNKGTHGRSGLIDKVRASKFSEADVEAMILEFLKLHVAPGTA
                     PLAGNTVHQDRRFMARYRPAFESYLHYRIVDVSTLKELARRWRPDVLAGVVKDSKHEA
                     LADVYESIEELRHYRRTFIRDSP"
     gene            complement(5934..7421)
                     /gene="glpK"
                     /locus_tag="VFV55_09390"
     CDS             complement(5934..7421)
                     /gene="glpK"
                     /locus_tag="VFV55_09390"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX4944553.1"
                     /translation="MKVILALDQGTTSSRAIVFDRAGKPISVVQQEFRQIFPQPGWVE
                     HDPEEIFATQRDVAARALAQAGLRSSDIAAVGIGNQRETTVLWDRATGRPLANAIVWQ
                     DRRTASRCDALRQAGHDAAFRDRTGLMLDPYFSGTKLAWLLDSIPGARARAERGELAF
                     GTIDSWLAWRLTDGGAHVTDASNASRTLLFNIHDLDWDEELLGILDIPRAVLPRVNAA
                     GEVVGKTRCAGLPEGIPIAAMSGDQQAALFGQACHRAGLAKNTYGTGCFLLMNTGERP
                     SASRHGLLTTVAWRRDEGAHYALEGGVFIGGAAVQWLRDGLGIIRSAAEVGPLAASVP
                     DAGGVYFVPALAGLGSPYWDPHARGTIVGLTRGTTRAHLARATLEAIAFQSAELLAAM
                     TADSGIALSELRVDGGATANDLLMQIQADLLGVPVVRPCVTETTALGAAYLAGIAVGF
                     WENEEEIASLWLRDRVFEPQIPRDFANARLEQWRRAVERSRDWAE"
     gene            7466..>8210
                     /locus_tag="VFV55_09395"
     CDS             7466..>8210
                     /locus_tag="VFV55_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944554.1"
                     /translation="MALVAAGELLVGLLDDLKKQADALKAQDVDRTESLRSNAVAVDH
                     ALRRAFLYMNDLGKQLNVVKVPCPFVYKLTTVGEVRGLVFKDFFADFRTKHFIDKDYY
                     GEVHLATRCSSDEVLTVRKGPDEMEKFRDILWQYNIEHKSEQYRDQRKVITHEIFEVK
                     CDFRVQAKFEGDHESGKLKIMAKNVGDFTIDVFQMTALEFNDKAVEEFAKYFIGRPNE
                     WVEIAKRSVLSPKAAAPSARPKPESRYPAP"
BASE COUNT         1331 a         2763 c         2847 g         1269 t
ORIGIN      
        1 atcgaaccta tgtcgaatcg cgtccggcgt cgcggctgat ggcaagttga tctggccaaa
       61 gacgaagaaa aacacaacca acaggccccc aacaccaggg cttcgctcat gaccgtgccg
      121 gcccgagcgt tcaccgccgt gccggcgtga tcacgacccg cgtctgtccc cttgcagaag
      181 tgctaacctg accgcatctc ctcctgtacg gaggtgcatg atgttggcga cgcgacctgc
      241 cgcggtggca gggtctttct atcccggcga tccttcgacg ctggccgtcg aagtggcggc
      301 ttgcctgcgc gacgcagtga agcctcttcc ggatgagcgg gcgccgaagg cgatcatcgc
      361 tccccacgca ggttacgttt actcgggccc gatcgcggcc agcgcctatg cgcgcgtggc
      421 tcccctgcgc gggaagatct cgcgcgtggt gctcgccggg cccgcccacc gggtccccgt
      481 tgctggcgcc gccgtgccgc aggcgggcgc cttcgccacc cccctcggcg acgtcccgct
      541 cgacgaggag gcgctcgcgc gcctgcgccg cctgccgttc gtcgaaacga gcgaccgttc
      601 gcacgcgctc gagcattcgc tcgaggtgca cctgcccttc ctgcagtccg tcctcggccg
      661 cttccggctc gtgcccgtgg tcgtgggcga tgtctctccg ggggagatgg ccgagttgct
      721 ggacgaggtc tggggcggcg acgagacgct cgtcgtcgtg agttccgatc tgtcgcacta
      781 cctgccctat ggggctgcgc gcgagaggga tcgcgccacg gcagcgtcga tcctcgcgct
      841 gtcgccccgg ctcgatccgc aggatgcctg cggcgcggca ccggtcaacg ggctgctcga
      901 ggtggcccgt cgccgcgggc tggaagtcga actcctggac ctgcgcaatt ccggggatac
      961 cgcggggaac cgcagccagg tggtcggtta cggtgcgttc atgttccgcg aaccggagcc
     1021 ccatcatgct tgagcccctg gatcgcggca ccgttctcgt gaccatcgcc cgcgaggcga
     1081 tcggcgaaac cttcgggttg gtcgttcccc ggcagcgtga cgaatcgtgg ctcgcccggc
     1141 ccgcggcgac tttcgtgacg ctgcggctgg acggggggct gcgcggctgc atcggctcga
     1201 tggagccgcg ccgcccgctc ggcgaggatg tcgcctgcaa cgcgcgcgcc gcggcgttct
     1261 ccgatccgcg cttcgagccg ctgaccatcg gggaattcgc ttccgtctgc ctcgaggtgt
     1321 ccgtgctttc gccgcgcacg ctgctcgtcg cgcagagcga gtcggaggcg gctgtcgcgc
     1381 ttcgtcccgg cgtggacggc gtctatctcg agtaccaggg gctcgcggcg actttccttc
     1441 cgcaggtgtg ggaggacctc ccggacccgg tcgacttcct cgccgcactt cgccacaagg
     1501 cgcggctgcc cgcgcgcttc tgggacccgg cgatccggct cacgcgatac accgtggaga
     1561 aatacggcga tggacacccg gcacattgac cccgtggagg gctcgcatca tccggcgcgg
     1621 tggtggcacc gcctggacga cgggcgaatc cagtgcgacc tctgcccgcg cgattgccgc
     1681 ctgcacgagg gccagcgcgg cgcctgcttc gtgcgcgcgc gctccggcga tgccatggtg
     1741 ctcaccacct acgggcgctc ctccggcttc tgcctcgatc ccatcgagaa gaagccgctg
     1801 aatcacttct tcccgggaac ctccgtcctt tccttcggca cggccggctg caatctcgcg
     1861 tgcaagttct gccagaactg ggacatcagc aagtcgcgcg acatggaccg gatgatggac
     1921 caggcgtcgc ccgaagccat cgcccgggcc gcgcgcgaaa ccggcgcgag gagcgcggcc
     1981 ttcacctaca acgatccggt catcttcgcc gagtatgcga tcgacgtggc cgacgcctgc
     2041 cgcgcggcgg gcatcgccac cgtggccgtg accaacggct acatgcacgc cgagccgcgc
     2101 cgcgagttct acgcgcgaat ggacgcggcc aacgtggacc tcaaggcatt caccgacggg
     2161 ttctacgtga agctcaccgg cgcgaagctg gctccggtgc tcgatacgct ctcctacctg
     2221 gtgcacgaaa cgaaagtgtg gaccgagatc acgacgctcc tcatccccgg gcacaacgac
     2281 tccgccgcgg agatcgaggc cgagagccgc tggatcatgc gcgagctggg gccggatgtc
     2341 ccggtgcact tcaccgcctt ccaccccgac tacaagatga cggatctccc ggtcacgccg
     2401 gcggccacgc tggagcgggc caggcgcatc gcgctgggcg aggggttgcg ctacgtgtac
     2461 acgggcaacg tccacgatcg cgagggcggc acgaccttct gcccgggctg ccacgcggcg
     2521 ctcgtggagc gcgactggca ccgcatcgac gcctatcgcc tcacggcgga cgggcattgc
     2581 cctgactgcg ggacggcgat cgcggggcgc ttcgaggcat tcgatccgaa gcggcagttc
     2641 gggcggcgcc gcatcccgct catgcttgcc ggcgtggcct agctgatcca gtccgggtgc
     2701 tggtcgtgca gctcgcgatg ctgctcgatg agcacctggt agaaggaaag ccgctccgcg
     2761 gagatggtgc ccttgccggc cgcctcgatg accgcgcagc ccggctcccg gaggtgcgcg
     2821 caatcattga acttgcacgc gccgaggaag ggccggaatt cgcgcatcgc ctccgcgatc
     2881 tggccttcgg tcaggtggaa gaggccgaac gactggaagc ccggcgtgtc gatgatggcg
     2941 gcgtcgcggt ccagccggaa cattcgagag aaggtcgtcg tgtgcgtgcc gatgcccagg
     3001 gaagcggaaa tctcgcccac gcgcgcgacc tcgttcatca ccagcgcgtt gacggtcttc
     3061 gacttgccca tgccgctctg cccgacgagc agggtgatgt tgccgtccag cagcggcagc
     3121 agcgcggagg tgtcgaactt cgccgacatc gagatcaccg ggtagccgat gcgctcccag
     3181 ggcaccagcg attcgcgcgc ggccttgtgc tcgggcaggt cggacttgtt gagcgcgatg
     3241 atcaccggaa tcccggcggc gtcgcaggcc accaggctca ggttgaggaa cgcctcgctg
     3301 aaggcgggcc gcggggcgag caggatcacc gcctggtcga cgttggcggc gagcgtcttt
     3361 tccttccact ggtcggagcg gaagaggata ttgcgccgcg ttcccacgcg ctcgatcgag
     3421 ccctgcgcgg agccggtcag gcgaacctcg acgaagtcgc cgcaggccgc gtcctgcttc
     3481 cggcccttgc gcgtgcaggc gatctgccgg cggtcggcga gctcgacgag gaagtcgcgg
     3541 ccgaaatcgg ccaccacgcg gccctcgacg acgacctgct ggtccctttc gcgtcgggtc
     3601 gtcatgggcg tggtggcgaa acgcggtcgg ggagggacat ccggtcctag aggtcgagca
     3661 gcgcgtcgat cttggccgcg cagatgaagt cgttctcgga gagcccgccg acggcgtgcg
     3721 tccagtacga aaccttgcac tggttatagc ctaccgcaag atcggggtga tggtcctcgc
     3781 ggtgcgagat ccaggcggtc gcgttaacga aggccatggt ctcgtggtag ttcgagaagc
     3841 ggtaggtctt cgcgatcgcc tcgccctcgc gcgcccagcc atcgagcccc ttgagcagcg
     3901 ggccgatctg cgcttcggtg aggggcgcca cgcccccctc gcacgggagg catttcttgc
     3961 gggcgagatc gctcatcggg ggccggggaa ttccttttcg gcgctacgca acggtgcagg
     4021 cggactttgc cgcgagccgc ctcagttggg cgatgcgcag agccgcgggc ggatgggaat
     4081 catacacgag ggaatgcagc gggtccggcg tcagcgtggc tgcgttgtcg cggtaaagct
     4141 tgccaagggc attcacgagg tcgtcggcgc tcgcgtggtt cgcggcgtat tcgtccgcct
     4201 cgaattcctg ccggcgcgac agcaggctgg cgagcggctg gaacgggaac gtgaagacgg
     4261 gaaccaccag catgaacgct ccgagcaggg cgcccgtggg cggcggcgac gagaggccga
     4321 gcgccacggc gaaagccggt tcgcgcgccg cccagcccag cacggccagc agcccgaagg
     4381 cgagcgcgaa gcgcaccgcg aggagcctcg ggatgtggcc gcgcgcgaag tggccaagtt
     4441 cgtgggcgag caccgcctcg atctcgccct gcgtgaggcg ctccatgagc gtgtcgaaga
     4501 acacgatccg cttggcccgc ccgaaccccg tgaagtaggc attgccgtgg ctcgagcgct
     4561 tcgacccgtc catgacgaac aggccctccg cccggaagcc gcagcgcgcc accagcgcct
     4621 cgatgcgcga gcgcagttcg ccttcctcca gcggcgtgaa gcggttgaac agcggcgcga
     4681 tccagcgcgg gaagatggcc acgaggacga gggagaaggc cacccacgcg atccaggccc
     4741 agacccacca ccacgccccg gtcgactcga tgaaccagca aacggccagc acgacgggcg
     4801 cgccgatcgc cacggccagc gccaggccct tcgcgatgtc cgcggcgaag gtggcgagcg
     4861 tcgtctggtt gaagccgtag cgcgcctcca cgcggaacgt gcgccaggcg tcgaacggga
     4921 gggaggtgat cgcgccgacc cccatcagcc cgaagacggt ggccaggtcg cgcgcatagc
     4981 cttccccgaa cagcgagcgc gaggcgccat cgaggagcgc gaggccgccc ccgacactca
     5041 gggcgaggag gagggcgccg tccacgacca ccgcctccac ggcggccacg cgaatcttgt
     5101 cgcgcgtgta gtccgccgcc tttgcgtgcg cctcgggcgg cacgagggcg gcgaaatcct
     5161 ccgggacgcg gtcgcgatgg cgcgcgacgt gacgggcgtg gcggcccatc agccagaggc
     5221 gccaggccag tgcggccagc gcgaagacga cgaagatcgt ggcgaatgtg ttcaaggtgg
     5281 ttaaatgaag gggacgctac cctttgcagt ccggatgcgc agtcagggcg cgcaaagggt
     5341 ggcgtcccct ttattgatac tctctcattc tatggcacag gaaaaagcac cgctcgtctg
     5401 ggtcgacatg gaaatgtcgg ggctggtccc cgatcgcgat cgcatcctcg aagtggcgat
     5461 cgtcgtgacc gacggggaac ttgccacgat cgccgaggcc cccgtctggg tgatacacca
     5521 gcccgacgaa gtcctggacg cgatggactc ctggaacaag ggcacccacg gtcgatccgg
     5581 tcttatcgac aaggtgcgcg cctcgaagtt cagcgaagcg gacgtcgagg cgatgatcct
     5641 ggagttcctg aagctccacg tggcgcccgg aaccgccccg ctcgcgggca acacggtgca
     5701 ccaggaccgg cgcttcatgg cgcgctacag gcccgccttc gagtcgtacc tgcactaccg
     5761 catcgtcgat gtctcgacgc tcaaggagct ggcgaggcgc tggcgtcccg acgtgctcgc
     5821 cggcgtcgtg aaggattcga agcacgaggc gctcgccgat gtctacgagt ccatcgagga
     5881 actgcgccac taccggcgca cgtttatcag ggacagtccc tgattagccg tccctactcg
     5941 gcccagtcgc gcgagcgctc gacggcgcgg cgccattgct cgaggcgggc gttggcgaaa
     6001 tcgcgcggga tttgcggctc gaatacgcgg tcgcgcagcc agagcgatgc gatctcctcc
     6061 tcgttttccc agaatcccac cgcaatgccg gcgaggtagg ccgcgccgag agccgtcgtc
     6121 tcggtgacgc acggccgcac cacgggcacg ccgaggagat ccgcctggat ctgcatcagg
     6181 aggtcgttgg cggtggcgcc accgtcgacg cgcagttccg agagtgcgat gccggaatcg
     6241 gcggtcatcg cggcgaggag ctccgcgctc tggaaggcga tcgcctcgag ggtcgcgcga
     6301 gcgaggtgcg cccgggtggt gccgcgagtg agcccgacga tggtgccgcg cgcgtgcgga
     6361 tcccagtacg gggagcccag tcccgcgaga gccggcacga agtacacgcc accggcatcg
     6421 ggcacgctcg ccgcgagcgg gccgacttcc gccgcgctgc ggatgatgcc gagcccgtca
     6481 cgcagccact ggacggcggc gcccccgatg aacacgccac cctcgagcgc atagtgcgcg
     6541 ccctcgtcgc ggcgccaggc caccgtggtg agtaggccat ggcgcgaagc cgaggggcgc
     6601 tcgccggtgt tcatcagcag gaagcagccc gttccgtagg tgttcttggc gagccccgcc
     6661 cggtggcagg cctggccgaa gagcgcggcc tgctggtccc ccgacatggc ggcgatcggg
     6721 atgccctcgg gaaggcctgc gcagcgcgtc tttcccacga cctcgccggc ggcgttcacc
     6781 cggggcagca cggcgcgcgg tatgtcgagg atgccgagga gttcctcgtc ccagtccagg
     6841 tcatggatat tgaagaggag ggtgcgcgag gcgttgctcg cgtcggtgac gtgggccccg
     6901 ccgtcggtga ggcgccaggc gagccaggaa tcgatggtgc cgaaggcgag ttcgccccgc
     6961 tcggcgcgcg cgcgggcgcc ggggatcgaa tcgagcagcc acgcgagctt ggtcccggaa
     7021 aagtacgggt cgagcatcag ccccgtccgg tcgcggaacg cggcgtcgtg cccggcctgc
     7081 cggagagcgt cacatcggga cgcggtgcgt cgatcctgcc agacgatggc gttggccagc
     7141 gggcggcccg tcgcccggtc ccagagcacg gtggtctccc gctggttgcc gatgccgacc
     7201 gcggcgatgt ccgacgagcg gagcccggcc tgggccagcg cccgggccgc gacatcgcgc
     7261 tgggtggcga agatttcctc ggggtcgtgc tcgacccacc ccggctgcgg gaagatctgg
     7321 cgaaattctt gctggacgac ggatatcggc ttaccggcgc gatcgaagac gatcgctcgg
     7381 gagcttgtcg tgccctggtc gagggcgaga atgactttca tcgggaaggc ctgatgccgc
     7441 gccgatgata cgataaaatc gccacttggc cctcgtggcc gcaggggagc ttctggtggg
     7501 acttctggac gatctcaaga aacaggcgga cgccctcaag gcgcaggacg tcgaccgcac
     7561 cgagagcctg cggtccaatg ccgtcgcggt cgaccatgcc ctgcgccgtg cgttcctcta
     7621 catgaacgac ctcggcaagc agctcaacgt ggtgaaggtg ccctgcccgt tcgtgtacaa
     7681 gctgacgacg gtgggcgagg tgcgcggact cgtcttcaag gatttcttcg ccgacttccg
     7741 caccaagcac ttcatcgaca aggactacta cggcgaggtg cacctcgcca cgcgctgcag
     7801 ctcggacgag gtgctcaccg tcaggaaggg cccggacgag atggagaagt tccgggacat
     7861 cctctggcag tacaacatcg agcacaagag cgagcagtac cgcgaccagc gcaaggtgat
     7921 cacgcacgag atcttcgagg tgaagtgcga cttccgcgtg caggccaagt tcgagggcga
     7981 ccacgagagc ggcaagctca agatcatggc gaagaacgtg ggcgacttca ccatcgacgt
     8041 gttccagatg accgcgctcg aattcaacga caaggccgtc gaggagttcg ccaagtactt
     8101 catcggccgg cccaatgagt gggtggagat cgccaagcgc tcggtgctca gcccgaaggc
     8161 ggccgcgccc tctgccaggc cgaagccgga atcccggtac cccgcgccga
//
LOCUS       DASUEV010000295         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_180128, whole genome shotgun sequence.
ACCESSION   DASUEV010000295 DASUEV010000000
VERSION     DASUEV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180128"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFV55_09400"
     CDS             complement(<1..748)
                     /locus_tag="VFV55_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX4944555.1"
                     /translation="MELKLPPLAETGETLTGAEIAIRCLQEEGVEFVFGYPGGAVLFI
                     YDALFKQKKVKHILVRHEQGALHAADGYARATGRPGVALVTSGPGVTNAVTGIATAYM
                     DSIPMVVITGQVPTRAIGEDAFQECDTVGITRPCVKHNFLVKDVNELAITIKKAFHIA
                     TTGRPGPVLVDIPKDVSQQPARFHYPDKIHLRSYSPVVKGHGGQIKKAMQLLLEAKRP
                     MIYVGGGAILGDASHEVTELVRLLDYPCTNT"
     gene            953..1519
                     /locus_tag="VFV55_09405"
     CDS             953..1519
                     /locus_tag="VFV55_09405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX4944556.1"
                     /translation="MASRKELSDFLSSVERRAFKQSVFSVQDEEAALDIVQDAMLKLA
                     ENYAARPPEELPLLFQRILQNGIRDHYRRSKVRSAWTSLWSSLGLTGEDGDADPLETF
                     AVEESGAIPAAPDARLEQSQMMAIIEEAVQALPERQRQAFLLRYWEDFDVSETAKAMG
                     CSEGSVKTHCSRAAHALAKTLRSKGITL"
     gene            1516..1893
                     /locus_tag="VFV55_09410"
     CDS             1516..1893
                     /locus_tag="VFV55_09410"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEX4944557.1"
                     /translation="MNEKTFGERLRPWLERSAGEVGEMQATRLRSARLRALDAWREPV
                     QILGVVTVGAGTVQTIKYSILQQALLWLPIVFLLATLVVKTLAPDVDIGELDAQLLTG
                     ELPIDAFLDKDFDQWLKSASGSF"
     gene            1917..2279
                     /locus_tag="VFV55_09415"
     CDS             1917..2279
                     /locus_tag="VFV55_09415"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX4944558.1"
                     /translation="MAGFPANAELKTPQTPWTRLSAEDRKVLTPLAPDWDNLPGYQQQ
                     RLMSAARRYPTMRPIQQERFQERIRDWAVMSPEQRKAARETFQGLKKLPPSKQHELRE
                     RWLEQKAAPAPGPRPERR"
     gene            2279..2704
                     /locus_tag="VFV55_09420"
     CDS             2279..2704
                     /locus_tag="VFV55_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX4944559.1"
                     /translation="MSAPESRAPPGVARRLACATYELLLLAALVLVATYPFIALAGES
                     TSGVRRHLLQAYVLTVVGAYLVGFWTHGGQTLAMKTWRIRLERADGGRVGVGRALRRY
                     VLAIAGIAALGIGFLWAFVDRDGQFLHDRLAGTRLVDAG"
     gene            complement(2718..>2984)
                     /locus_tag="VFV55_09425"
     CDS             complement(2718..>2984)
                     /locus_tag="VFV55_09425"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX4944560.1"
                     /translation="LWQKVIYPLAAIVMMVLSIPFAIQSQRAGGTGGKIVLGIMLGLG
                     FHFAGRLFAHVGLLNDWPAGFSAGMPTLLFLGVAAAGLAHAERR"
BASE COUNT          503 a         1014 c          986 g          481 t
ORIGIN      
        1 gcgtattcgt gcacggatag tcgagaagcc gcaccagctc ggtgacctcg tgcgaagcat
       61 cgccgaggat ggccccgccc cccacgtaga tcatggggcg cttcgcctcc agcaggagct
      121 gcatcgcctt cttgatctgc ccgccgtgcc ccttgaccac cgggctgtag gagcgcaggt
      181 ggatcttgtc ggggtagtgg aagcgcgcgg gctgctggga gacgtccttg gggatgtcga
      241 cgagcacagg gcccgggcgt ccggtggtcg cgatatggaa cgccttcttg atcgtgatcg
      301 cgagttcgtt cacgtccttg acgaggaagt tgtgcttcac gcaggggcgc gtgatcccca
      361 ccgtgtcgca ttcctggaag gcatcctccc cgatggcccg cgtgggcacc tggcccgtga
      421 tgaccaccat gggaatggag tccatgtagg cggtcgcgat tccggtcacc gcgttcgtca
      481 cgccgggccc gctggtgacg agggcgaccc cgggccgtcc ggtggcgcgc gcatagccgt
      541 ccgccgcgtg cagcgccccc tgctcgtgcc gcaccaggat gtgcttgacc ttcttctgct
      601 tgaacagggc gtcgtagatg aacaggacgg cgcccccggg atagccgaat acgaactcga
      661 ccccctcctc ctgcagacag cggatggcaa tctcggcacc cgtcagggtt tcgccggttt
      721 ccgcgagcgg aggcagcttt agttccataa ggccttgtaa atcgtgaagt tttgaaaagg
      781 ggacctagaa cattagccgg gagacagggc ttggtcaagc cgaatgaaga gataagcggc
      841 ttccgccgcc ggccagcccc accccggtcc tttgctatag tcgcggcgtc gcgggccgaa
      901 tgcgctcccc gcccttgcgc gggaagggcc ccgccgcccg cgggaggacc ccctggcctc
      961 aagaaaagaa ctgtccgact tcctctcttc cgtggagcga cgcgccttca agcagagcgt
     1021 gttctcggtc caggacgaag aagcggccct cgacatcgtc caggacgcga tgctcaagct
     1081 cgccgagaac tatgcggccc ggccgccgga ggaactgccg ctcctcttcc agcgcatcct
     1141 gcagaacggc atccgcgacc actatcggcg aagcaaggtg cgctcggcgt ggacgtcgct
     1201 gtggtcgtcg ctgggcctca ccggcgagga cggcgacgcc gatccgctcg agacgttcgc
     1261 cgtcgaggag tcgggcgcca tccccgccgc ccccgacgct cgcctcgagc agtcgcagat
     1321 gatggcaatc atcgaggaag cggtgcaggc gcttccggaa cgtcaacggc aagccttcct
     1381 tctgcgttat tgggaagact tcgatgtcag cgaaacggcc aaggccatgg ggtgctcgga
     1441 aggcagcgtg aagacacact gctcccgggc tgcgcacgcg ttggcaaaga ccctgagatc
     1501 aaaagggata accctatgaa tgaaaagact tttggtgaaa ggctgcgacc ctggctcgag
     1561 cggtcggccg gcgaagtggg ggaaatgcag gccacccgcc tgcgctcggc gcgcctgcgg
     1621 gcactcgatg cctggcgcga gccggtgcag atcctcggcg tcgtgaccgt gggcgccggc
     1681 accgtgcaga ccatcaagta ctcgatcctg cagcaggcgc tcctgtggct tccgatcgtc
     1741 ttcctgctgg cgaccctggt cgtcaagacc ctcgcgccgg acgtggatat cggcgaactc
     1801 gacgctcaac tcctcaccgg cgagctcccg atcgacgcct tcctcgacaa ggatttcgac
     1861 cagtggctca agagcgcttc cggatccttc taggggcact ggtcgcgtgc atcgcgttgg
     1921 ccggatttcc ggccaacgcg gaactcaaga ccccgcagac gccctggaca agactgtcag
     1981 ccgaggaccg caaggtgctc acgccccttg cgcccgactg ggacaacctg cccggctacc
     2041 agcagcagcg cctcatgtcg gccgcccgcc gttacccgac gatgcgcccc atccagcagg
     2101 agcgtttcca ggagcgcatc cgcgactggg cggtgatgag ccctgaacag cgcaaggcgg
     2161 cccgcgaaac cttccagggc ctcaagaagc tgccgccgtc caagcagcac gaattgcgcg
     2221 agcgctggct cgagcagaag gcagcgccgg cgccggggcc caggcccgag cgccgctgat
     2281 gtccgcgccg gaatcgcgcg cgccgccggg tgtggcgcgc cggctcgcct gcgcaaccta
     2341 cgaattgctc ctcctcgcgg cgctggtcct cgtcgcgacc tatcccttca tcgcgctcgc
     2401 gggcgaatcc accagcgggg tgcgccggca cctgctgcag gcctacgtcc tcaccgtggt
     2461 cggcgcctac ctcgtgggtt tctggacgca tggcgggcag acgctggcca tgaagacctg
     2521 gcgcattcgc ctggaaaggg cggatggcgg gcgcgtgggc gtaggccgcg cgctccgccg
     2581 ctacgtcctt gccattgccg gcatcgcggc gctggggatc gggtttctct gggccttcgt
     2641 cgatcgcgac gggcagttcc tgcacgaccg cctggcgggc acccgtctcg tggacgcggg
     2701 ctgaggcgaa ggccccgtca gcggcgctcg gcgtgcgcca ggcccgccgc cgccacgccc
     2761 aggaagagca gcgtgggcat gccggccgag aaccccgccg gccagtcgtt gagcaggccc
     2821 acgtgcgcga aaagccgccc cgcgaaatgg aacccgagac cgagcatgat gccgaggacg
     2881 atcttgcctc ccgtcccgcc ggcgcgctgg gactggatcg cgaacgggat ggagagcacc
     2941 atcatcacga tcgccgcaag gggatagatc accttctgcc aaag
//
LOCUS       DASUEV010000296         4361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1004114, whole genome shotgun sequence.
ACCESSION   DASUEV010000296 DASUEV010000000
VERSION     DASUEV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004114"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFV55_09430"
     CDS             complement(<1..421)
                     /locus_tag="VFV55_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX4944561.1"
                     /translation="MTLKQMAPTKKIHSFHPPQRTLLGPGPSEINPRVMAAMSLPAIG
                     YLDPVFVEMMEELKSLLRYAYQTKNALTFPVSGPGSVGMEYCFVNMVSPGDKVIVCRN
                     GVFGGRMIENVERAGGTAIVVEDAWGEPCDPGKLEDAL"
     gene            complement(510..1421)
                     /gene="ttcA"
                     /locus_tag="VFV55_09435"
     CDS             complement(510..1421)
                     /gene="ttcA"
                     /locus_tag="VFV55_09435"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX4944562.1"
                     /translation="MPAATFPAQDTRKADFEAAKRRKRIRRLVGEAIADFSMIGPGDK
                     VMACLSGGKDSYALLDILLHLRDCAPVPFEVVAVNLDQRHPGFPENVLPDYLASRGVP
                     FHIVVQDTHSVVKRVIPEGKTMCSLCSRLRRGVLYRVARELGATRIALGHHRDDILET
                     FFLNLFHNGKIKAMSPKLVSDDGRHVVIRPLAYVEERDLAAYAEDRAFPIIPCDLCGS
                     QENLQRKQVKAMLREWEKKYPGRVISIFSALTRVTPSHLMDRTLFDFANLVADGNPQP
                     DGDTAIDPEELPDLSQGTIVRFTPGRG"
     gene            complement(1431..2171)
                     /locus_tag="VFV55_09440"
     CDS             complement(1431..2171)
                     /locus_tag="VFV55_09440"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX4944563.1"
                     /translation="MTQPAVLVTGGARRVGAAIVRALHAAGASVIVHCHRSCADAERL
                     LADLENARAGSTAIVQADLLDTPRLPRLVEEAAARFGRLDGLVNNASSFHATPFDGLE
                     AEAWEDLVGTNLRAPLFLSKAAALHLRASRGAIVNIVDIHAERPLAGFLAYSVAKSGL
                     AGLTRALALELAPEVRVNGVSPGAILWPEDGEAFPPGERERITVQTPLRRTGSPEDVA
                     GAVKYLLFDAPFVTGQVLAVDGGRSIAL"
     gene            2276..3457
                     /locus_tag="VFV55_09445"
     CDS             2276..3457
                     /locus_tag="VFV55_09445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX4944564.1"
                     /translation="MPSQVEFSLPHPAPEAAAHSHELAEHIAGTIAQEGGWIPFSRYM
                     ELALYAPGFGYYAAGSRKIGAEGDFVTAPEISPMFARCLAMQVRQVLEQTGGEVLELG
                     PGSGVLAADLFAELKAHGAAPSRYRLLEVSPELRERQRACIAGRHPGDIGRFEWIDRL
                     PQRIDGVVLANEVLDVLPCALVIRAGAQVLERGVVVTEAGFGWEDMAIPEGELKRRAQ
                     AVIPAGDYPYLTEVNLAAEALVRSVAAALGRGLALFIDYGFAQNEYYHPQRSMGTLRC
                     HYRHRIHNDPFFLPGLQDITAHVDFTAMALAAQSAGAEVMGYTTQAHFLISCGLAVFV
                     SEGDAGMTLSRLKATSAVHRLIGPSEMGELFKVLGVARGVEPPLLGFQSARPLALGIA
                     D"
     gene            complement(3504..>4361)
                     /locus_tag="VFV55_09450"
     CDS             complement(3504..>4361)
                     /locus_tag="VFV55_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX4944565.1"
                     /translation="SIQEGMTPIIVMSVIALLFGIHMVMAIGGADMPVVVSMLNSYSG
                     WAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRNFISVIAGGFGGETSAPAG
                     GGAAQPAGEVVPVNAQEAADLLREAKSVVIVPGYGMAVAQAQHTVFEITRRLREMGKT
                     VRFGIHPVAGRMPGHMNVLLAEAKVPYDIVFEMDEINGDFPDTDVSMVIGANDIVNPS
                     AQEDPSSPIAGMPVLEVWKAHTSIVMKRSMASGYAGVDNPLFYKDNNRMLFGDAKKML
                     DEVLAALKD"
BASE COUNT          670 a         1471 c         1506 g          714 t
ORIGIN      
        1 tgagggcgtc ctcgagcttg ccggggtcgc agggctcgcc ccacgcgtcc tcgaccacga
       61 tcgcggtgcc gccggcgcgc tcgacgttct cgatcatgcg gccgccgaac acgccgttcc
      121 tgcacacgat caccttgtcg cccggggaga ccatgttcac gaagcaatac tccatgccca
      181 cggagccggg ccccgagacg gggaaggtga gcgcgttctt cgtctggtag gcgtagcgca
      241 gcagcgattt cagctcttcc atcatctcga cgaacacggg atcgaggtag ccgatggccg
      301 gcaggctcat tgccgccatc acgcgcggat tgatctccga cggtcccggg ccgaggagcg
      361 tgcgctgcgg cgggtggaag gaatggatct tcttggtggg tgccatttgc ttgagcgtca
      421 tttcggggtc cttgtcattg gggcgtcttg ccgacgtgcc cccatggaaa atcgaagcgg
      481 gaaagggaag cggagccggt cgatgcgggt caacctcgcc cgggagtgaa gcgcacgatc
      541 gttccctgcg agaggtcggg cagctcttcc ggatcgatgg cggtgtcgcc gtcgggttgc
      601 gggttgccgt cggccaccag gttcgcgaag tcgaagagcg tgcggtccat gaggtgcgag
      661 ggcgtgacgc gcgtgagggc actgaagatc gagatcacgc gcccggggta cttcttctcc
      721 cattcgcgca gcatcgcctt cacctgcttt cgctgcaggt tctcctgcga gccgcacagg
      781 tcgcacggga tgatggggaa ggcacggtcc tcggcgtagg cggcgagatc gcgttcctcg
      841 acgtaggcaa gcgggcggat caccacgtgg cggccgtcgt cggagacgag cttgggcgac
      901 atcgccttga tcttgccgtt gtggaaaagg ttgaggaaga acgtctcgag aatgtcgtcg
      961 cggtggtggc cgagcgcgat ccgggtcgcg cccagctccc tggccacgcg gtagagcacc
     1021 ccgcggcgaa ggcgcgagca aaggctgcac atcgtcttgc cttcggggat gacccgcttc
     1081 acgacgctgt gcgtgtcctg gaccacgatg tggaagggca ccccgcgtga agcgaggtag
     1141 tcgggcagca cgttttccgg aaagccgggg tggcgctggt cgaggttcac ggcgaccacc
     1201 tcgaacggca cgggggcgca gtcgcgcagg tgcagcagga tgtcgaggag cgcgtagctg
     1261 tccttgccgc cggaaaggca cgccatcacc ttgtcgcccg gcccgatcat cgagaagtcg
     1321 gcgatcgcct cgcccacgag gcggcggatg cgcttgcggc gcttggcggc ctcgaaatcg
     1381 gccttgcgcg tgtcttgcgc aggaaaggtg gcggcaggca tgggatccgg ctagagggcg
     1441 atcgatcgtc cgccgtcgac cgccaggacc tggccggtga cgaacggggc gtcgaaaagc
     1501 aggtatttta ccgcgcccgc gacgtcctcc gggcttcccg tgcggcgaag gggcgtctgc
     1561 acagtgatcc gctcgcgctc gccgggtggg aacgcctcgc cgtcctcggg ccacaggatg
     1621 gcgccgggcg acaccccgtt cacgcgcacc tcgggggcca gttccagggc cagggcgcgc
     1681 gtgaggcccg ccagcccgct cttggcgacg ctgtaggcga ggaaccccgc caggggccgc
     1741 tcggcatgga tgtcgacgat gttcacgatg gcgcctcgcg aggcgcgcag gtgcagtgcc
     1801 gcggccttcg agaggaagag gggcgcgcgc aggttggtgc cgaccaggtc ttcccaggcc
     1861 tcggcctcga ggccgtcgaa cggcgtcgca tggaaggagg aggcgttgtt caccaggcca
     1921 tcgagccggc cgaaccgggc cgcagcctct tccaccagcc gcggcagccg gggggtgtcg
     1981 agcaggtctg cctggacgat cgccgtcgat ccggcccttg cgttctcgag gtccgcaagg
     2041 aggcgttcgg catcggcgca ggaacggtgg caatgcacga tgacgctcgc gccggccgcg
     2101 tgcagggcgc gcacgatcgc cgcccccacc cgccgggcgc cgcccgtcac gagaaccgca
     2161 ggctgggtca tgatgctata tttcctccga gaatcgcccc accccgatcg ggcggcgaca
     2221 gaggcgagag tttagcgaaa tccgcgaacg atccccgacg gcgccgccat ccccgatgcc
     2281 ttcccaagtc gaattcagcc ttccgcatcc cgctcccgaa gcggcggcgc actcgcatga
     2341 gctcgccgag cacatcgccg gcacgatcgc gcaggaaggc ggctggatcc cgttctcccg
     2401 ctacatggag cttgccctct atgcgccggg cttcgggtac tacgcggcgg ggtcgcgcaa
     2461 gatcggggcc gagggggact tcgtcacggc ccccgagatc tcgcccatgt tcgcccgctg
     2521 cctcgccatg caggtgcgcc aggtgctgga gcaaaccggt ggcgaagtcc tcgagctggg
     2581 accgggatcg ggcgttctcg ccgccgacct tttcgccgag ctcaaggccc acggggcggc
     2641 gccctcgcgc taccgcctcc tcgaagtcag ccccgagctg cgcgaacgcc agcgcgcgtg
     2701 catcgccggg cgacatcccg gcgacatcgg gcggttcgag tggatcgatc gcctgccgca
     2761 gcgcatcgac ggcgtggtcc tcgcgaacga ggtgctcgac gtccttccct gcgccctcgt
     2821 gattcgcgcc ggcgcgcagg tcctcgaaag gggcgtggtg gttaccgagg ccggattcgg
     2881 ctgggaggac atggccattc ccgaaggcga gctcaagcgc cgcgcacagg ccgtgatccc
     2941 ggccggggac tacccgtacc tcacggaagt gaacctcgcc gccgaggcgc tcgtccgctc
     3001 ggtcgcggcc gccctgggcc gcggactcgc gctgttcatc gactatgggt tcgcgcagaa
     3061 cgagtactac cacccgcagc gctcgatggg caccctgcgc tgccattacc gccaccgcat
     3121 ccacaacgac ccgttcttcc tgccggggct gcaggacatc accgcgcacg tggacttcac
     3181 cgccatggcg cttgccgcgc aatcggccgg cgccgaggtc atgggataca cgacgcaggc
     3241 gcatttcctc atctcgtgcg ggctggcggt cttcgtctcg gaaggcgacg ccggcatgac
     3301 gctgtcccgc ctcaaggcga cgagcgccgt gcaccggctc atcggaccgt cggagatggg
     3361 ggagctgttc aaggtgctcg gcgtcgctcg cggcgtggag ccgccgttgc tcggcttcca
     3421 gtccgcgcgg ccgctcgcac tcggcatcgc cgattaggcg gggcgcccgg tcatcacatg
     3481 gcggggcctg cggggcccgc gcttcagtcc ttcagcgccg ccagcacctc gtcgagcatc
     3541 ttcttcgcgt cgccgaacag catccggttg ttgtccttgt agaagagcgg gttgtccacg
     3601 ccggcgtagc ccgaggccat gctgcgcttc atcacgatcg aggtgtgcgc cttccacacc
     3661 tcgagcaccg gcatcccggc gatggggctc gacgggtctt cctgggcgct ggggttcacg
     3721 atgtcgttgg cgccgatgac catggagacg tcggtgtccg ggaagtcgcc attgatctcg
     3781 tccatctcga acacgatgtc gtagggcacc ttggcctcgg ccagcaggac gttcatgtgg
     3841 cccggcatgc gcccggcgac cgggtggatg ccgaagcgca ccgtcttgcc catctcgcgc
     3901 aaccgcctgg tgatctcgaa gaccgtgtgc tgcgcctggg cgaccgccat gccgtagccc
     3961 ggcacgatga ccacgctctt tgcttccctg agcaggtccg cggcttcctg cgcgttcacc
     4021 ggcacgacct cgccggcggg ctgcgccgcg ccgccgcccg cgggagcgga ggtctcgccc
     4081 ccgaacccgc ccgcgatcac gctgatgaag ttgcggttca tcgcgcgaca catgatgtac
     4141 gacaggatcg cgccggagga gcccaccagc gcgccggtca cgatcagcag atcgttcgag
     4201 agcatgaagc cggtggccgc cgcagcccat ccggagtagc tgttcaacat tgagacgacc
     4261 accggcatgt ccgcgccgcc gatcgccatc accatgtgga tgccgaagag gagcgcgatc
     4321 acgctcatca cgatgatcgg cgtcattccc tcctggatgg a
//
LOCUS       DASUEV010000297         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_304802, whole genome shotgun sequence.
ACCESSION   DASUEV010000297 DASUEV010000000
VERSION     DASUEV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304802"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="ilvC"
                     /locus_tag="VFV55_09455"
     CDS             complement(<1..645)
                     /gene="ilvC"
                     /locus_tag="VFV55_09455"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX4944566.1"
                     /translation="MKVYYDKDADLSLVKGKKVTIIGYGSQGHAHAQNLKDSGVKVTV
                     GLRADGASWDKAKKAKLEVKEVGAAVKGADLVMILLPDENHPAVWKESIEPNIKKGAA
                     LAFAHGFNIHFGQIQPRADLDVIMVAPKGPGHLVRSTYTQGGGVPALIAVHQDASGKA
                     KDLALSYACAIGGTRGGVIETNFREETETDLFGEQTVLCGGIVELIKAGYETLVE"
     gene            complement(673..1164)
                     /gene="ilvN"
                     /locus_tag="VFV55_09460"
     CDS             complement(673..1164)
                     /gene="ilvN"
                     /locus_tag="VFV55_09460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX4944567.1"
                     /translation="MRHILSILVENEAGALSRVAGLFSARGYNIESLTVAPTEDATMS
                     RMTIVTTGSDEIVEQITKQLNKLVDVVKVVDLSEGKHIERELMLVKVRASGKDREEMK
                     RTADIFRGRVIDVTDKSYTIELTGTTDKLDAFLEALDKGAILETVRTGVSGIGRGEWV
                     LKA"
     gene            complement(1261..>2701)
                     /gene="ilvB"
                     /locus_tag="VFV55_09465"
     CDS             complement(1261..>2701)
                     /gene="ilvB"
                     /locus_tag="VFV55_09465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX4944568.1"
                     /translation="SIPMVVITGQVPTRAIGEDAFQECDTVGITRPCVKHNFLVKDVN
                     ELAITIKKAFHIATTGRPGPVLVDIPKDVSQQPARFHYPDKIHLRSYSPVVKGHGGQI
                     KKAMQLLLEAKRPMIYVGGGAILGDASHEVTELVRLLDYPCTNTLMGLGSFPASDRRF
                     VGMLGMHGTVEANMAMQNCDVLLAVGARFDDRVIGNPAHFVAGSAGRKIVHIDVDPSS
                     ISKRVRVDVPIVGHVKDVLTELLALLKASKDKPDAAALAAWWAQIATWRAKDCLKYDR
                     NSAIIKPQFVIEKLWEVTKGDAYITSDVGQHQMWAAQFYRFEKPRRWINSGGLGTMGV
                     GLPYAMGVKLAFPKADVACVTGEASIQMCIQELSTCKQYHLPVKLVNLNNRYMGMVRQ
                     WQEFFHGNRYSESYMDALPDFVKLAEAYGHVGMRIEKPGDVEGALREAFALKDRLVFL
                     DIITDQTENVFPMVPGGKGMSEMILAEDL"
BASE COUNT          428 a          866 c          879 g          528 t
ORIGIN      
        1 ctccaccagc gtctcgtagc ccgccttgat gagttcgacg attccgccgc agagcaccgt
       61 ctgctcgccg aagaggtcgg tctccgtctc ctcgcggaaa ttcgtctcga tgaccccgcc
      121 gcgcgtgccg ccgatcgcgc aggcgtagga gagcgcgaga tccttggcct tgcccgacgc
      181 gtcctggtga accgcgatga gggcgggtac gcctccgccc tgcgtgtacg tcgagcgcac
      241 gaggtgcccg ggacccttgg gcgcgaccat gatcacgtcc aggtccgccc gcggctggat
      301 ctggccgaaa tggatgttga agccgtgcgc gaaggcgagc gcggcgccct tcttgatgtt
      361 gggctcgatc gactccttcc agacggcggg gtggttctcg tccgggagca ggatcatcac
      421 gaggtccgcg cccttgaccg cggcgccgac ttccttcacc tcgagcttgg ccttcttcgc
      481 cttgtcccac gaggcgccgt ccgcgcgcag ccccaccgtg accttcacgc ccgagtcctt
      541 gaggttctgc gcgtgtgcgt gcccctgcga gccgtagccg atgatcgtga ccttcttgcc
      601 cttcacgagg gagagatccg cgtccttgtc gtagtacacc ttcatgttcg tatcctcaga
      661 tttgcagaaa agtcaggctt tgagcaccca ctcgccccgg ccgatgcccg agacgccggt
      721 gcggacggtt tccaggatcg cgcccttgtc gagcgcctcg aggaaggcat cgagcttgtc
      781 ggtggtgccg gtgagctcga tggtgtagga cttgtcggtc acgtcgatga cgcggccgcg
      841 gaagatgtcc gcggtgcgct tcatctcctc gcggtccttt ccggaggcgc gcacctttac
      901 gagcatcagc tcgcgctcga tgtgcttgcc ttcggagagg tcgaccacct tcaccacgtc
      961 caccagcttg ttcagctgct tggtgatctg ctcgacgatc tcgtcggagc cggtggtgac
     1021 gatcgtcatg cgcgacatcg tcgcatcctc ggtgggggcg acggtgagcg actcgatgtt
     1081 gtagccacgg gcggagaaaa ggcccgcgac gcgcgaaagc gcgcctgctt cattttcgac
     1141 gaggattgag aggatgtggc gcatggcgaa gggtggttta acgaggtgct ggtgcgaggg
     1201 aagaaatggg gacgctaccc ttttccgggg tatcgggaca agggtagcgt cccctttccc
     1261 ttacaggtct tcggcgagga tcatctccga catgcccttt ccgccgggca ccatcggaaa
     1321 gacgttttcc gtctggtcgg tgatgatgtc gaggaacacg aggcggtcct tgagcgcaaa
     1381 ggcctcgcgc agcgcgccct cgacgtcgcc gggcttctcg atgcgcatcc cgacgtggcc
     1441 gtaggcctcg gcgagcttca cgaaatccgg cagcgcgtcc atgtacgact cgctgtagcg
     1501 gttgccgtgg aagaattcct gccactggcg caccatcccc atgtaccggt tgttgaggtt
     1561 cacgagcttc accggcaggt ggtactgctt gcaggtggag agttcctgga tgcacatctg
     1621 gatggacgcc tcgcccgtga cgcaggcgac gtcggccttc gggaaggcga gcttcacgcc
     1681 catggcgtag ggcagcccaa cccccatcgt ccccaggccg ccggaattga tccagcgccg
     1741 cggcttttcg aagcggtaga actgcgcggc ccacatctgg tgctggccga cgtccgaggt
     1801 gatgtaggca tcgcccttcg tgacttccca gagcttctcg atcacgaact gcggcttgat
     1861 gatcgcggaa ttgcggtcgt acttcaggca atccttggcg cgccacgtcg cgatctgcgc
     1921 ccaccaagcg gccagcgccg cggcatcggg cttgtccttc gaggccttga ggagcgcgag
     1981 caactcggtc aggacgtcct tcacgtgacc cacgatcggc acgtcgacgc gcacgcgctt
     2041 ggagatcgac gacgggtcga cgtcgatgtg cacgatcttg cggccggcgc tgcccgcgac
     2101 gaagtgcgcg ggattgccga tcaccctgtc gtcgaagcgc gcccccacgg cgagcagcac
     2161 gtcgcagttc tgcatcgcca tgttggcttc cacggtcccg tgcatgccga gcatgccgac
     2221 gaagcgcctg tccgaggccg ggaacgagcc cagtcccatg agcgtattcg tgcacggata
     2281 gtcgagaagc cgcaccagct cggtgacctc gtgcgaagca tcgccgagga tggccccgcc
     2341 ccccacgtag atcatgggac gcttcgcctc cagcaggagc tgcatcgcct tcttgatctg
     2401 cccgccgtgc cccttgacca ccgggctgta ggaacgcagg tggatcttgt cggggtagtg
     2461 gaagcgcgcg ggctgctggg agacgtcctt ggggatgtcg acgaggacgg gacccgggcg
     2521 tccggtggtc gcgatatgga acgccttctt gatcgtgatc gcgagttcgt tcacgtcctt
     2581 gaccaggaag ttgtgcttca cgcaggggcg cgtgatcccc accgtgtcgc attcctggaa
     2641 ggcatcctcc ccgatggccc gcgtgggcac ctggcccgtg atgaccacca tgggaatgga
     2701 g
//
LOCUS       DASUEV010000298         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1004970, whole genome shotgun sequence.
ACCESSION   DASUEV010000298 DASUEV010000000
VERSION     DASUEV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004970"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..466
                     /locus_tag="VFV55_09470"
     CDS             98..466
                     /locus_tag="VFV55_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX4944569.1"
                     /translation="MAEIDIRRTHNLGMKAAREAADRMAEDLGKKFGLSGGWTGNTHH
                     FDRPGVTGSLHLTDKDLHLTVKLGLLLRMMRGPLEAAIIRELDSLFASHPQAGAGKAK
                     APARKTVSPKTGSGGRKKAG"
     gene            complement(423..1127)
                     /locus_tag="VFV55_09475"
     CDS             complement(423..1127)
                     /locus_tag="VFV55_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944570.1"
                     /translation="MPVAPAGTAPESLRRHLERLREMRGREAHGHMSPRLAEVKAWQA
                     SRLARTYADLSANPRYAKATEFFLGDLYGAKDFSGRDEAMLRIYPVMVRTLPRGAVET
                     ASLAIEVDALSEELDRRVAACIEAGQVGEASYARAYRQAGTRAERMRQMELIGEVGRR
                     LDALVVKPIFYLTLKLMRQPARIAGLEDLQTFLERGFEAFRHMAGADEFLATIASRET
                     AILNRLFSGHPSPFSG"
     gene            1287..2687
                     /locus_tag="VFV55_09480"
     CDS             1287..2687
                     /locus_tag="VFV55_09480"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEX4944571.1"
                     /translation="MTPPLEAEFARHRDAFARQPFSVSPARIELLDRLHALVKDHMGE
                     WADAISRDFGNRSRHETQLLEIFPCLEAIRHARRHVRRWMRPERRSTSFWFLPGGSSV
                     VPQPLGVVGIVVPWNYPLYLAIGPLVPALAAGNRAMVKMSETTPATGELFSRLAVQYF
                     AGGEVTVANGGPEVAQAFCRLPFDHLLFTGSTGIGRQVMRAAAENLTPVTLELGGKSP
                     AIVGRGFPIDEAASRVMFGKCLNAGQTCIAPDYALVPEESVEAFVEGARRAATALYPT
                     LAANPDYTAIVDGRHRERLARLIGDAVAKGARAIEINPAGEDLAAVGKRAPTLLTGVD
                     DSMTVMREEIFGPVLPVVAYRRLEDAIAFVNARPRPLALYVFEHDRKSVDLVVSNTVS
                     GGVTVNETILHIAQDDLPFGGVGASGMGHYHGREGFDTFSKRKAVFRQSRLNGLKLFR
                     PPYGSRFESLVRLLLR"
BASE COUNT          483 a         1050 c          979 g          510 t
ORIGIN      
        1 ggtggtgcta tgcgtggtcc ccggcatcgc cacctggctt ccggacatgc tgatgggccc
       61 gggccgctga gacggccgca cttcaacgga gcacttcatg gcagaaatcg acatccggcg
      121 tacccacaac ctgggcatga aagcggcacg tgaggcagcc gaccggatgg ccgaggacct
      181 cgggaagaag ttcggcctga gcggcgggtg gacgggaaac acccaccact tcgaccggcc
      241 gggcgtgacg ggcagccttc acctcacgga caaggacctg cacctcaccg tgaagctggg
      301 attgctgctc cggatgatgc gcggcccgct cgaggcggcg atcatccgtg aactcgactc
      361 gctctttgcc agccatccgc aggccggcgc cgggaaggcc aaggctcccg caaggaagac
      421 cgtcagcccg aaaacgggct cgggtggccg gaaaaaagcc ggttgaggat cgccgtctcg
      481 cggctcgcaa tggtcgcgag gaattcgtcc gcgcctgcca tgtggcgaaa cgcctcgaag
      541 ccccgttcga gaaaggtctg cagatcctcg agtccggcta tccgcgcggg ctggcgcatg
      601 agcttgagcg tcaagtagaa gatcggcttc accaccaggg cgtccagccg gcggccgacc
      661 tcgccgatca actccatctg gcgcattctt tcggcgcggg tccccgcctg cctgtaggcg
      721 cgggcgtagc tcgcctcgcc gacctgccct gcctcgatgc aggccgccac gcgccggtcc
      781 agttcctccg acaacgcgtc gacctcgatc gccagggacg cggtctccac cgcgccgcgc
      841 ggcagcgtgc ggaccatgac cgggtagatg cgcagcatcg cctcgtcacg gccgctgaag
      901 tccttcgccc cgtagaggtc accgaggaag aactccgtgg ccttggcata gcggggattg
      961 gccgacaggt cggcgtaggt gcgggccagc cggctggcct gccacgcctt cacttccgca
     1021 agccgcggcg acatgtgtcc atgggcctcc cggccgcgca tctcgcgcag tcgctcgaga
     1081 tgccggcgaa gcgattcggg cgcggtgccc gctggcgcaa caggcatggt gggcaggttg
     1141 cctcgcagtg gatacgaccg ctattctaac gagtgattga aacgctcgtt tgaatctccg
     1201 gtgctggcag ggctattgca atatagttgt gttttatcat ttgtttaagg ttgcttctta
     1261 aagttattta taactaggga atccacttga ctccacccct cgaagccgaa ttcgcgcgcc
     1321 accgcgacgc cttcgcccgt cagccgtttt ccgtttcccc tgcccgcatc gagctcctcg
     1381 accggctcca tgcgctggtg aaggatcaca tgggcgagtg ggccgatgcg atatcgcgcg
     1441 atttcggcaa ccggtcccgg cacgagacgc agctcctcga gatctttccc tgtctcgagg
     1501 caatccgcca cgcgcggcgt cacgtccggc gctggatgag gccggagcgc cggtccacca
     1561 gcttctggtt cctgccgggc ggttcgagcg tcgtgccgca gccgctcggc gtggtgggca
     1621 tcgtcgtgcc gtggaactac cccctttacc tcgccatcgg tcccctggtg ccggccctcg
     1681 cggcgggtaa ccgcgcgatg gtgaagatgt ccgaaacgac ccccgccacc ggcgaactct
     1741 tctcgcgcct ggccgtgcaa tacttcgcgg gtggagaagt caccgtggcg aacggcgggc
     1801 cggaggtggc gcaggcgttc tgccgcctgc ccttcgacca cctccttttc accggctcga
     1861 ccggcatcgg gcggcaggtg atgcgtgcgg ccgccgagaa cctcaccccg gtcacgctgg
     1921 aactcggcgg caagtctccg gcgatcgtcg ggcgcggctt ccccatcgac gaggcggcga
     1981 gccgcgtgat gttcggcaag tgcctcaacg ccggacagac ctgcatcgct ccggactacg
     2041 cgctcgtgcc ggaggaatcc gtcgaagcct tcgtggaagg cgcgcggcgg gccgcgaccg
     2101 cgctctatcc cacgcttgcc gccaaccccg attacacggc catcgtcgac gggcgccacc
     2161 gcgagcgcct tgcgcgcctg atcggcgacg ccgtcgccaa gggggcgcgc gcgatcgaga
     2221 tcaatcccgc cggcgaggac ctggccgccg tgggaaagcg ggccccgaca ctgctcaccg
     2281 gcgtcgacga ttccatgacg gtgatgcgcg aggaaatctt cggacccgtg ctgcccgtcg
     2341 ttgcctatcg ccgcctcgag gacgccatcg ccttcgtgaa tgcccggccg cgccccctgg
     2401 ccctctacgt gttcgagcac gaccggaagt ccgtggacct ggtggtctcg aacaccgttt
     2461 ccggcggcgt cacggtgaac gagacgatcc tgcacatcgc gcaggacgac ctccccttcg
     2521 gtggcgtggg cgcgtccggg atggggcact accacggccg cgaggggttc gacaccttct
     2581 cgaagaggaa ggccgtcttc cgccagtcgc gcctcaacgg cctcaagctc ttccggcccc
     2641 cttatggaag ccgcttcgaa agcctcgttc gcctgttgct gcgctgaagc gccgtgatca
     2701 cgcggcgcca gttcaggcag gtcggcctga ggcaatcccc aagcgcgtcg cgccgtcccg
     2761 atatactgtc ccgacgaaac gccgccgggc ctggtgggaa acgccgccgg gcctggaggc
     2821 ccctcacttg ccacgagcga gatcggggaa ccgccatggg cgacatgatt gcactcgagc
     2881 gccgctatgc ggcgcgcaat tacgatccgc tgccggtcgt cctgaccgag ggcgccggtg
     2941 cctggctcac cgacgagcaa gggcgtcgtt acctcgacat gctgtcggcc tattccgccg
     3001 tgagcttcgg ccacgcgcac cc
//
LOCUS       DASUEV010000299         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_90998, whole genome shotgun sequence.
ACCESSION   DASUEV010000299 DASUEV010000000
VERSION     DASUEV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90998"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFV55_09485"
     CDS             <1..322
                     /locus_tag="VFV55_09485"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX4944572.1"
                     /translation="VASVAVRGEATGSATGWRADCVATAKRDLKGGEVLDGEGGYTVY
                     GKLMPASDSLKAGGLPIGLAHKVKLLKDVPAGRPVSWADVEFDGNAEAVKIRREMERE
                     GADA"
     gene            319..1494
                     /gene="metC"
                     /locus_tag="VFV55_09490"
     CDS             319..1494
                     /gene="metC"
                     /locus_tag="VFV55_09490"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX4944573.1"
                     /translation="MKKITKLIHAGRTAAHPPATVNLPVARASTVLFESLDHLAGTQK
                     LFDAGERIATYGIVNMPQRNAFEELVCALEGGHRAVTVPSGLAAVAVAIIAAVKAGDH
                     VLMVDSVYGPSRQFCDRTLAKFGVRTTYYDPLASGEQVAALMRPETSVVYVESPGSLT
                     FEVQDIPAIAKAAHAQGALVIADNAWATGLLCPVMDYGVDLVVQPATKYLGGHSDVIL
                     GAVVANERAWPLLHATSRDLGQTASPDDIYLMVRGMRTLAIRLEKQGAAALDIARWLQ
                     GRPEVKRVLHPGLESDPGHALWKRDFKGSSGLFGVELQPCTRAQVAALVDHCGHFGIG
                     YSWGGFESLIIPANLRHGRQVRPWDGGPLIRLHIGLEDPADLMADLDAAFARWRAAA"
     gene            1499..2401
                     /locus_tag="VFV55_09495"
     CDS             1499..2401
                     /locus_tag="VFV55_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX4944574.1"
                     /translation="MTPELRAALERTVMDATRGDWSLTAAEPVSGGCIHTALCLEGAD
                     SGGKTKHFAKLAPIERAPMLLAEAQGLEAIHAAGAVRVPAVVARDDDGETAWLILEWL
                     ELVPLSAASGARLGAALAAQHRVPQAKFGWEKDNYIGSSPQLNGWSEDWLAFWSNRRI
                     HAQLRMAMANRYPSRMIDRGERLLTDCAVFFRTHGPVPSLLHGDLWGGNASALGDGTP
                     VVFDPAVYCGDREADLAMTELFGGFPRDFHSAYRNAWPLDDGYAVRRDFYNLYHVLNH
                     ANLFAGAYVEQGSRMIERLLSEIR"
     gene            2404..3114
                     /locus_tag="VFV55_09500"
     CDS             2404..3114
                     /locus_tag="VFV55_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX4944575.1"
                     /translation="MNLAITKWALGPVLLLQGRRVRRRTPVLPEPAGPRKGFTGEGAP
                     LRLLVLGDSAGAGVGAATQEQALTGRIVPRLAHRFRVEWRLVARTGATTASTLRHLER
                     IEPFAADAVVISLGVNDVTGNVGVARFLELQAKLHALLRERFGARLILATGLPPMGRF
                     PALPQPLRWYLGARSHELDRALAASLPDGRGAEYLALAGELDAAHMAADGFHPGPAVY
                     DAWAEAAARRIAAAFAGA"
     gene            3201..>3840
                     /locus_tag="VFV55_09505"
     CDS             3201..>3840
                     /locus_tag="VFV55_09505"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944576.1"
                     /translation="MQGYKVETNTHRIGKLDYQVRALSDRRQYSDPDGEAQRAGVTPA
                     SWPMFGVLWPAGIALAEAMSVIPVAGKRILEVGCGLGLASLVLRRRGADITASDHHPL
                     AEAFMRHNTDLNDLAPIPFSHSSWGETDPGIGRFDLLIGADVLYEHDHPQLLAAFVAL
                     HANRTVQVVLADAGRGHCGRFNTCMEAQGYTRVEKRVRFGGDWPPGNCGRLLT"
BASE COUNT          573 a         1286 c         1410 g          571 t
ORIGIN      
        1 cgtcgcttcg gtcgccgtgc gcggagaggc cacgggcagc gccaccggat ggagggccga
       61 ttgcgtggcc acggcgaagc gcgacctgaa gggcggcgag gtgctcgatg gagagggggg
      121 ctacacggtc tacgggaaac tcatgccggc ctccgactcg ctcaaggcgg gcggccttcc
      181 gatcggcctc gcgcacaagg tgaagctgct gaaggacgtg cccgcggggc ggcccgtgtc
      241 ctgggccgac gtggaattcg acgggaatgc cgaggcggtg aagatccgcc gcgaaatgga
      301 acgcgagggc gccgacgcgt gaaaaagata acgaagctga tccatgcagg gcgaaccgcg
      361 gcgcacccgc cggcgaccgt gaacctgccg gtggcgcgtg cctccaccgt gctcttcgag
      421 agcctggatc acctggccgg gacgcagaag ctcttcgacg cgggcgagcg gatcgcgacc
      481 tacggcatcg tgaacatgcc gcagcgcaac gccttcgagg agctcgtctg cgcgctggaa
      541 ggcggccatc gtgcggtgac cgtgccttcg ggtcttgcgg ccgtggcggt ggcgatcatc
      601 gcggcggtga aggcgggcga ccacgtcctg atggtcgatt cggtgtacgg cccgtcgcgg
      661 cagttctgcg accgcacgct ggcgaagttc ggcgtgcgca ccacctatta cgatccgctc
      721 gccagcggag agcaggtggc ggcgctgatg cggcccgaga cctcggtggt gtacgtggag
      781 agccccggct cgctcacctt cgaggtccag gacatcccgg ccatcgcgaa ggccgcgcat
      841 gcgcagggcg ccctggtgat cgcggataac gcgtgggcga ccgggctcct ttgccccgtg
      901 atggactacg gcgtggacct cgtggtgcag ccggcgacca agtatctcgg cgggcattcg
      961 gacgtgatcc tgggcgccgt cgtcgccaac gaacgcgcct ggccgctgct gcacgccacg
     1021 tcgcgcgacc tgggccagac cgcgagcccc gacgacatct acctgatggt tcgcggcatg
     1081 cgcacgctcg ccatccgcct cgaaaagcag ggcgccgcgg cgctcgacat cgcgcgctgg
     1141 ctgcaagggc gtcccgaagt gaagcgcgtg ctgcacccgg ggctggagag cgatccgggc
     1201 cacgcgttgt ggaagcgcga cttcaagggc tcgtcggggc tgttcggcgt cgagctgcag
     1261 ccgtgcacgc gcgcgcaggt ggcggccctc gtggaccact gcgggcactt cggcatcggc
     1321 tacagttggg gcggcttcga gagcctcatc attcccgcga acctgcgcca cgggcgccag
     1381 gtgcggccat gggacggcgg cccgctcatc cgcctgcaca tcgggctgga ggacccggcg
     1441 gatctcatgg ccgacctcga cgccgcgttc gcgcggtggc gcgccgcggc gtgaaggcat
     1501 gaccccggag cttcgcgcgg cgctcgagcg caccgtgatg gacgcgacgc gcggcgactg
     1561 gtcgctgacc gccgcggagc cggtatcggg cggttgcatc catacggcgc tgtgcctcga
     1621 gggcgcggac agcggcggga agacgaagca ttttgcgaag ctcgcgccga tcgagcgtgc
     1681 cccgatgctc ctggcggagg cgcagggact cgaggcgatc cacgccgccg gggcggtgcg
     1741 cgtgcccgcc gtggtcgcgc gcgacgatga cggcgagacg gcgtggctca tcctggaatg
     1801 gctggaactc gtgccgcttt cggccgcatc cggcgcgagg ctcggggcag cactggccgc
     1861 gcagcaccgc gtgccgcagg cgaagttcgg gtgggagaag gacaactaca tcggctcctc
     1921 gccgcaactc aatggctgga gcgaggactg gctggcattc tggagcaaca ggcgcatcca
     1981 cgcgcaactg cggatggcga tggccaatcg ctatccgtcc aggatgatcg accgcggcga
     2041 gcggctgctc accgattgcg cggtcttctt ccgcacgcac gggcccgtgc cctcgctcct
     2101 gcacggcgac ctgtggggcg gcaacgcctc ggcgctcggc gacggcacgc ccgtcgtctt
     2161 cgaccccgcg gtgtactgcg gcgaccgcga ggccgacctc gcgatgacgg agctcttcgg
     2221 cgggttcccc cgggatttcc attcggcgta tcgcaacgcc tggccgctcg atgacggcta
     2281 cgcggtgcgc cgcgacttct acaacctgta ccacgtgctc aaccacgcga acctcttcgc
     2341 gggcgcgtat gtcgagcagg gctcgcggat gatcgagcgg ctgctctccg agatccgcta
     2401 gcggtgaacc tggcgatcac gaagtgggcg ctcggcccgg tgctgctgct gcagggccgg
     2461 cgagtgcggc gccggacgcc ggtcctgccg gagcccgccg gcccgcgcaa gggattcact
     2521 ggcgaaggcg cgccgcttcg cctgctggtg ctgggcgact cggcgggcgc cggcgtgggt
     2581 gccgcgacgc aggaacaggc cctcacgggc cggatcgtgc cccggcttgc gcaccgcttc
     2641 cgcgtcgaat ggcggctcgt cgcgagaacg ggagcgacga ccgcatcgac cctgcgccac
     2701 ctggagcgga tcgagccctt cgccgccgat gccgtcgtga tctccctcgg cgtgaacgac
     2761 gtgaccggaa acgtgggcgt ggcccgcttc ctcgagttgc aggcgaagct gcatgcgctg
     2821 ctccgcgagc gcttcggcgc gcggctcatc ctcgcgacgg gattgccgcc gatggggcgc
     2881 tttcccgcgc tgccgcagcc gcttcgctgg tacctggggg cgcggtcgca tgagctcgat
     2941 cgtgcgctgg cggcatcgct gcccgacggc aggggcgcgg aatatctcgc gctggccggc
     3001 gagctggatg ccgcgcacat ggccgccgat ggattccatc cggggccggc ggtttacgac
     3061 gcgtgggccg aggcggcggc gcgaaggatc gccgcggcgt tcgcaggcgc ctgaccggga
     3121 aagcgtgcca atcggcgtac catgcaggaa ggggcttttt ccgacgagaa ggccacccat
     3181 cgcattcact gggattccgc ttgcagggat acaaggtcga gacgaacacg caccgcatcg
     3241 ggaagctcga ctaccaggtg cgtgcgctgt cggaccggcg gcagtattcc gaccccgatg
     3301 gagaagcgca gcgcgctggc gtgacgccgg cctcctggcc gatgttcggc gtgctatggc
     3361 cagcgggcat tgcgctcgca gaagcgatga gcgtgatccc cgtcgcgggt aagcgaatcc
     3421 tggaggtggg atgcgggctg ggcctagcga gcctggtgct gcggcggcgc ggcgcggaca
     3481 tcaccgcgag cgaccaccac ccgctggccg aggcgttcat gcgccacaac accgatctca
     3541 acgatctcgc accgatcccg ttcagccact cgtcgtgggg agaaacggat cccggcatcg
     3601 ggcgcttcga cctgctgatc ggcgccgacg ttctttacga gcacgaccac ccgcagttgc
     3661 tcgcggcatt cgtggcgctg cacgccaacc gcaccgtgca ggtcgtgctg gccgacgccg
     3721 ggcgcgggca ttgcgggcgg ttcaacacgt gcatggaggc gcagggctac acgcgcgtcg
     3781 agaagcgggt gcggttcggc ggcgactggc cgccgggaaa ctgcggaaga ctcctcacct
//
LOCUS       DASUEV010000300         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1587398, whole genome shotgun sequence.
ACCESSION   DASUEV010000300 DASUEV010000000
VERSION     DASUEV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587398"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFV55_09510"
     CDS             complement(<1..193)
                     /locus_tag="VFV55_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4944577.1"
                     /translation="MLRALALRDFVIVDTLDLDLATGFTALTGETGAGKSILVDALGL
                     VLGARADPGVIRPGAERADL"
     gene            complement(208..1083)
                     /locus_tag="VFV55_09515"
     CDS             complement(208..1083)
                     /locus_tag="VFV55_09515"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX4944578.1"
                     /translation="MFPPFKAVAVVGKSDASVLPDKLDQVVAVLRSRGAAVLMDTRTA
                     EASRTPPDEAMALEDLPARADLAVVVGGDGTLLSCARLMAAHGVPLIGVNLGRLGFLT
                     DIPADKVSETLSALFDGDYVAEERLMLAARVLRAGREVFATHAMNDVVVSRAGTGSMI
                     EFAVHVDGEFIYSLRADGIIVATPTGSTAYALSAGGPILHPGLSAIALVPISPHTLSN
                     RPVAIHSGSRIDIVLVRGPGARANFDVQSHVDLAANDVVGIATAAKPAILLHPRGYRY
                     FSMLRDKLRWNERNA"
     gene            1166..2182
                     /gene="hrcA"
                     /locus_tag="VFV55_09520"
     CDS             1166..2182
                     /gene="hrcA"
                     /locus_tag="VFV55_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX4944579.1"
                     /translation="MLNDRAQALLKILVERYIVDGQPVGSRALARASNLELSPATIRN
                     VMADLEEMGFVSSPHTSSGRVPTPKGYRLFVDTLLTVRPLEDVERAQIEESLLPDDPH
                     RVVAAASQILSDLTHFAGIVISPRRRATLRHVEFFHLSEKRVLLIIVGSDGEVQNRVL
                     LTDRPYSPAQLTEAANAINQNYAGQDFASIATRLRLELLDLHRDISALMRAAIETGGQ
                     AMHDPGDGVVLSGERNLLEVEDLFPDRARLRKLFDLFERKAQLLGLLDESERAEGVKI
                     FIGGDSQLVPLDECSIVTAPYEVDGKVVGTVGVIGPTRMAYERVIPIVDVTAKLISSV
                     LSQR"
     gene            2183..>2902
                     /gene="hemH"
                     /locus_tag="VFV55_09525"
     CDS             2183..>2902
                     /gene="hemH"
                     /locus_tag="VFV55_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX4944580.1"
                     /translation="MAYLPETPYAHGSPSRTGILLVNLGTPDEPTAKAVRRYLAEFLW
                     DPRVVEIPRPVWWMILRFILLTRPAKSAAKYLLVWTKDGSPLRVHLGRQVQLLRGYLG
                     ERVKSPLAVAGACRYGNPSIAAGLAELRGQGCDRIVVLPLYPQYAASTTGSTMDAVGA
                     ALAAMRNVPAIRTVRHFHDHPAYVKAVARSVNDYWMKHGRPDRLVMSFHGLPRFTLDK
                     GDPYHCQCRKSARLIATELGWN"
BASE COUNT          457 a         1026 c          978 g          441 t
ORIGIN      
        1 tgagatcggc gcgctccgct ccgggacgga tgacgccggg atcggcgcgg gccccgagga
       61 cgagcccgag cgcatccacg aggatcgact tgcccgcgcc cgtctctccg gtgagcgcgg
      121 tgaatcccgt ggcgaggtcg aggtcgagcg tatccacgat cacgaagtcg cgaagcgcga
      181 gggcgcggag catggaggac cgccgcctca ggcgttgcgc tcgttccagc gcaacttgtc
      241 gcgcagcatc gagaaatagc ggtagccgcg cgggtgcagg aggatcgccg gcttcgcggc
      301 ggtggcgatg ccgaccacgt cgttggccgc caggtcgacg tgcgactgca cgtcgaagtt
      361 ggcgcgtgcg cccggaccgc gcacgaggac gatgtcgatg cgcgaaccgc tgtggatcgc
      421 caccggacgg ttggagagcg tatgcggcga gatcgggacg agcgcgatgg cggagagccc
      481 cgggtggagg atgggccctc ccgcggaaag cgcataggcc gtggaccccg tcggcgtcgc
      541 gacgatgatg ccgtcggcgc gcaggctgta gatgaattcc ccgtccacgt gcacggcgaa
      601 ctcgatcatg ctgccggtgc ccgcgcggct taccaccacg tcgttcatcg cgtgggtcgc
      661 gaacacctcg cggccggcgc gaagcacgcg cgcggcaagc atcaggcgtt cctcggcaac
      721 gtagtcgccg tcgaacaggg ccgagagggt ctcggacacc ttgtcggccg gaatgtcggt
      781 gaggaagccc agccgcccga ggttcacgcc gatcagcgga acgccgtggg cggccatgag
      841 gcgggcgcag gacagaagcg ttccgtcccc gcccacgacc accgcgaggt cggcgcgggc
      901 aggcaggtcc tcgagcgcca tcgcctcgtc gggcggcgtg cgggacgcct cggcggtgcg
      961 ggtatccatg agcacggcgg ccccgcggga gcgaaggacg gcgaccacct ggtcgagctt
     1021 gtccgggagc accgaggcgt cgctcttgcc gacgacggcg acggccttga atggcgggaa
     1081 catgggcccc attagatcac agcgttcccg tcaaacaaag gcgaatcccg gctccggccg
     1141 agccgaattt tcgtacaatg tccccatgct gaacgatcgc gcgcaggccc tgctcaagat
     1201 cctcgtcgaa cgctacatcg tggacggcca gccggtcggc tcgcgggcgc tggcgcgggc
     1261 ctcgaatctc gagctgtcgc cggccacgat ccgcaatgtc atggccgacc tcgaggagat
     1321 ggggttcgtc tcgagcccgc atacgtcctc ggggcgcgtg cccacgccaa agggctatcg
     1381 cctgttcgtc gacaccctgc tcaccgttcg ccccctggag gatgtcgagc gcgcgcagat
     1441 cgaggaaagc ctcctgccgg acgacccgca ccgggtggtc gcggccgcct cgcagatcct
     1501 gtcggacctc acccatttcg ccgggatcgt gatctcgccg cggcgccggg cgaccctccg
     1561 gcacgtggag ttcttccatc tctccgagaa gcgcgtcctc ctcatcatcg tcggctccga
     1621 cggcgaagtg cagaatcgcg tcctcctcac cgaccggccc tactcgccgg cgcagctgac
     1681 cgaggcggcc aacgccatca accagaatta cgcgggccag gacttcgcct cgatcgcgac
     1741 ccgcctgcgc ctcgaactcc tcgacttgca ccgggacatc tcggcgctca tgcgcgccgc
     1801 catcgagacg ggcggccagg ccatgcacga tcccggagac ggcgtggtgc tctccggcga
     1861 gcgcaacctg ctcgaggtcg aggacctctt cccggatcgt gcccggctgc gcaagctctt
     1921 cgacctgttc gagcgaaagg cgcagctcct gggcctgctg gacgagagcg agcgggccga
     1981 aggcgtgaag atcttcatcg gcggcgattc ccagctcgtc ccgctcgacg aatgctccat
     2041 cgtgacggct ccctacgagg tggacggaaa ggtcgtcggc acggtcggcg tgatcggccc
     2101 gacgcgcatg gcctacgagc gcgtcatccc catcgtcgac gtgacggcga agctcatttc
     2161 ctccgtgctg tcgcagcgct gaatggccta cctccccgaa acgccctacg cgcacggctc
     2221 cccgtcgcgc accggcatcc tgctggtgaa cctcggcacg ccggacgagc ccacggcgaa
     2281 ggcggttcgc cgctacctcg ccgaattcct gtgggatccc cgcgtcgtcg agattccgcg
     2341 cccggtctgg tggatgatcc tgcgcttcat cctcctcacg cgcccggcaa agagcgcggc
     2401 caagtacctt ctggtgtgga ccaaggatgg ctcgccgctg cgcgtccatc tcgggcggca
     2461 ggtgcagctg ctgcgcgggt acctcggcga gcgcgtgaag tctcctctcg cggtggcggg
     2521 cgcctgccgc tacggcaatc cttccatcgc cgccggtctc gcggagcttc gcggccaggg
     2581 ctgcgaccgc atcgtcgtcc tgccgctcta cccgcagtac gccgcaagca cgacgggttc
     2641 gaccatggac gccgtcggcg cagcgctcgc cgccatgcgc aacgttccgg ccatccgcac
     2701 cgtgcgccac ttccacgacc acccggctta cgtgaaggcc gtcgcgcggt cggtgaacga
     2761 ttactggatg aagcacgggc gccccgaccg cctggtgatg agcttccacg ggctgccgcg
     2821 cttcacgctc gacaagggcg atccgtacca ctgccagtgc cgcaagagcg cgcgcctcat
     2881 cgccaccgag ctcggctgga ac
//
LOCUS       DASUEV010000301         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_523625, whole genome shotgun sequence.
ACCESSION   DASUEV010000301 DASUEV010000000
VERSION     DASUEV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523625"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFV55_09530"
     CDS             <1..840
                     /locus_tag="VFV55_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX4944581.1"
                     /translation="GRDDQQGPAIAAYIAGELKGKKVAIIDDKTAYGEGLANEVEKTL
                     KGAKVAIVARERTTDKETDFKAILTKIKAKNPDVIFHGGMDATGGPMLKQARELGIKA
                     VFAFGDGACTDEMSKLAGAASEGMACSQAGLPAVASSKEFQDSFKAKFGEIKQYAPYF
                     YDGAMAIVEAMKKANSVDPAKFTPEVFNVSFQGATGKVEFDAKGDRKDAEMTIFRMGK
                     DGKIAPVAIVKGGVSTPFAQPAAAAPAAAAPAAAAPAAAPAEGKKDEGKKDDDKKKKK
                     GGC"
     gene            1018..2016
                     /locus_tag="VFV55_09535"
     CDS             1018..2016
                     /locus_tag="VFV55_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4944582.1"
                     /translation="MDTFLQQVVNGLTLGCVYAVVALGYTMVYGIVQLINFAHGEVVM
                     IGCMVAFSVIVALAPSGLPPLVIVGAGMLVAVPVCMAVGYVVERAAYRPLRNAPRLAP
                     LITAIGVSIVLQHLALLVWSRNIIAFPQIIELKLYHLGTSETSAAISNVQVAIIVTSI
                     AMMGGLLALVYRTRLGIAMRATSQNPLVAGLMGIDINRVISATFVIGAALGAVAGVMV
                     GSYYGIAHYQMGFLLGLKAFSAAVLGGIGNLAGAVLGGILMGLIESLGAGYVGDFTNV
                     CLLDKFLPGFAEVCAASPESSLFGSNYKDVYAFIVLILVLVFRPSGLLGERVGDRA"
     gene            2059..2441
                     /locus_tag="VFV55_09540"
                     /pseudo
     CDS             2059..2441
                     /locus_tag="VFV55_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
BASE COUNT          470 a          861 c          856 g          451 t
ORIGIN      
        1 ggacgcgatg accagcaggg cccggccatc gccgcctaca tcgccgggga gctgaagggc
       61 aagaaggtcg ccatcatcga cgacaagacg gcctacggcg aaggcctcgc caacgaggtc
      121 gagaagacgc tcaagggcgc caaggtcgcg atcgtcgccc gagagcgcac caccgacaag
      181 gaaacggact tcaaggcgat cctcaccaag atcaaggcga agaatcccga cgtcatcttc
      241 cacggcggca tggacgcgac cggcggcccg atgctcaagc aggcgcgcga gctgggcatc
      301 aaggcggtct tcgctttcgg cgacggcgcc tgcacggacg aaatgtcgaa gctcgccggc
      361 gcggcctccg aaggcatggc ctgctcgcag gcggggctgc cggccgtcgc ttccagcaag
      421 gagttccagg actccttcaa ggccaagttc ggcgagatca agcagtacgc gccctacttc
      481 tacgacggcg ccatggccat cgtcgaggcg atgaagaagg ccaactcggt cgacccggcc
      541 aagttcacgc cggaagtgtt caatgtgtcc ttccagggcg cgaccggcaa ggtcgagttc
      601 gacgccaagg gtgaccgcaa ggatgcggaa atgaccatct tccgcatggg caaggacggc
      661 aagatcgccc cggtggccat cgtcaagggc ggcgtgtcga cgccgttcgc ccagcctgcc
      721 gcggcagcgc ccgcggccgc ggcgcctgcg gctgcggcac ccgctgcggc ccccgccgag
      781 ggcaagaagg acgaagggaa gaaagacgac gacaagaaga agaagaaggg cggctgctga
      841 taaccggcgc gccgggcccg cgcacgcctg cacctcggca aacggggtga ccgaagaaga
      901 agcgaagaag gacgagaaga agaagaaacc tgtctcgact ggaacggcac cggcgacggt
      961 gccgtttttt ttcgcccact tctcgtagaa tccgcctgac gaccaaggag cggcgccctg
     1021 gataccttcc tccagcaggt ggtgaacgga cttacgctcg gctgcgtgta cgccgtcgtg
     1081 gcgctgggct acacgatggt ctacggaatc gtgcagctca tcaacttcgc gcacggcgag
     1141 gtggtgatga tcggctgcat ggtcgccttc tcggtgatcg tggcgctcgc gccttcgggc
     1201 ctgccgccgc tcgtgatcgt gggcgcggga atgctggtcg ccgtgccggt atgcatggcc
     1261 gtcggttacg tggtcgagcg cgcggcctac cggccgctgc gcaacgcgcc tcgcctcgcc
     1321 cccctcatca ccgcgatcgg cgtgtccatc gtgctgcagc acctggcgct cctcgtctgg
     1381 agccgcaaca tcatcgcctt tccgcagatc atcgaactga agctctacca cctggggaca
     1441 tcggagacca gcgccgccat ctccaacgtt caggtcgcca tcatcgtcac ctcgatcgcg
     1501 atgatgggcg ggctcctcgc gctcgtgtac cgcaccaggc tcggcatcgc catgcgcgcc
     1561 acctcgcaga acccgctggt ggcagggctc atgggcatcg acatcaaccg cgtgatctct
     1621 gccaccttcg tgatcggcgc cgcgctcggc gcggtggccg gcgtgatggt cggcagctat
     1681 tacggaatcg cgcattacca gatgggcttc ctgctcggcc tcaaggcgtt cagcgccgcg
     1741 gtgctgggtg gaatcggcaa tctcgccggc gccgtgctgg gcggcatcct catggggctg
     1801 atcgagtcgc tgggcgcggg ctacgtcggc gacttcacca acgtgtgcct gctcgacaag
     1861 ttccttcccg gctttgcgga ggtgtgcgcg gcgagccccg agtcgagcct gttcggcagc
     1921 aactacaagg acgtgtacgc gttcatcgtc ctcatcctcg tgctcgtctt ccggccctcg
     1981 gggctgctcg gcgagcgtgt cggcgaccgg gcctaggcgg gcgttatgga gttacgccac
     2041 ccaaaggcag ggacacggat ggacacggat attggcttgg tgggagagag ggacgtttcc
     2101 cgaagcgtga tcggcgctgc actccgagtc tcgaattcgc tcggcgtcgg ctttctcgag
     2161 aaggtctatg aaaacgcgct cgcggtcgaa ttgcggcccg ccggtcacga ggtcgagcag
     2221 caacgcacga tcgacgtacg ataccgcggc gaaatcgtcg ggatctatca ggcggatctg
     2281 ctggtcgagg caagctcgtc gtcgaactca aggtcgcgga tgcgttgtca tccacgcaca
     2341 aggcgcaatg cctcaattgc ctcaaggcga cacgtcttca caccggcctc ctcatcaact
     2401 tcggacgccc ccgccttgaa gtccgtcgtg tcctttcctg atccttgccc gaattcatcc
     2461 gtgttcatcc gtgtgcatcc gtgttcatcc gtgttccaaa ggtttgcgtg accatgggca
     2521 ccctggaccg cgtccgcact gacccccgcc tcaagtgggc gggcctcgcc ttgatcgcga
     2581 tggccctcct cgccctgccg ttcgcactgg cgatggcagg cacggcgtgg gtgaggat
//
LOCUS       DASUEV010000302         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1467040, whole genome shotgun sequence.
ACCESSION   DASUEV010000302 DASUEV010000000
VERSION     DASUEV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467040"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VFV55_09545"
     CDS             complement(<1..1632)
                     /locus_tag="VFV55_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX4944583.1"
                     /translation="MFKNCLLALACLALTATAGASDADILAARDAARRGNVKALESLR
                     TRTAGHLLASYPEYWLLSATLETAPPDDIRAFLAKRADGPLADQLRRDWLKLLGAAGQ
                     WDLFRKEFPGLASDDVEVACYSLQERLARNDADAPAEARSLWLAGREAPAACAPAFEA
                     AARERLIGPPEAWERIRKLLAAGLVKDARRANAFLPTADQMNEMALERAQAEPDRYLA
                     AEKSRSPSRATREILLFAVSRLARKRPEDAAAQLKSRIGALGPDDARYGWAQVAHAAA
                     QQHHGRALEWYAEAGEGPFTDAQLQWKARAALRAGNWSSVREAVNLMSPAESREPSWR
                     YWLARALAAEGARPASETILGQLARERNVYGLMAAEDLGAVEDPDWTTRVVADEDLAR
                     VRAIPAIGRAIALYKLDMDTEAFREWVFGLRAIDDRGLLAASELATQAGLADRAIGAA
                     DRTASLHDYTRRYPIPHREPLAAAARQWNVDEALLFGLIRQESRFNPEALSRAGASGL
                     MQLMPATARWVAKQIPVASYRPALLAQPEVNVAMGT"
     gene            1788..2531
                     /locus_tag="VFV55_09550"
     CDS             1788..2531
                     /locus_tag="VFV55_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX4944584.1"
                     /translation="MGEVMTAQFWGGLMAIIGVNIVLSGDNAVVIALAARGLPPKQQK
                     AAIFWGSSAAIVMRIILTIVAVELLRLPYLKLVGAALLLWIAVQLLLPEHDSDDGAPT
                     SGSLWAAIRTILIADLVMSLDNVIGVAAAAKGSVTLLVLGLAISIPLVIFGSTLLLKL
                     MERYPVIITGGAALLGWVAGDMAVTDPSIKDWIDANMSWLHTVAPAAGAIAVVAVGRW
                     LAARAEAAEESEPAVDLAKADRSNGGAGP"
     gene            2578..3006
                     /locus_tag="VFV55_09555"
     CDS             2578..3006
                     /locus_tag="VFV55_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX4944585.1"
                     /translation="MLRILVAVDGSESSDRAVAHLLKKLGWYKEKIEVHLLNVQAALP
                     SQVGLYAPAAEQLQGFHREQGEAALASARAILDGAKVGYVTHIGIGEPAHVIAHYAKE
                     KSVDQIVMGARGLGSIAGLVMGSVTTKVLGLTDVPVLVIK"
     gene            3232..>3649
                     /locus_tag="VFV55_09560"
     CDS             3232..>3649
                     /locus_tag="VFV55_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944586.1"
                     /translation="MKQRPFAKGLLAAVVASAFSALTPALAWEPTKTVEFIVPAGTGG
                     GADQMARLSQSIIAKHNLMKQSLVVVNKGGGAGAEGFLDVKGAKGDPHKIIITLSNLF
                     TTPLATGVPFNWKDLTPVRMLALDQFVLWVNKDDPAK"
BASE COUNT          554 a         1223 c         1320 g          552 t
ORIGIN      
        1 ggtgcccatc gcgacattca cctccggctg cgcgaggagc gcgggccggt acgaggcgac
       61 cggaatctgc ttggccaccc atcgcgccgt cgccggcatg agctgcatga ggccgctggc
      121 gcccgcgcgc gagagggcct cggggttgaa gcggctctcc tggcggataa ggccgaagag
      181 gagggcttca tcgacgttcc actggcgcgc ggcggcggcg agaggttcgc gatggggaat
      241 cgggtagcga cgcgtgtagt cgtggaggga ggcggtgcgg tccgcggcac cgatggcgcg
      301 gtcggcaagg ccggcttgcg tggcgagctc tgaggcggcg agcaggcccc ggtcgtcgat
      361 cgcgcgcagg ccgaacaccc attcgcggaa ggcctccgta tccatgtcga gcttgtagag
      421 ggcgatggcg cggccgatgg cgggaatggc gcgtacccgc gccaggtcct cgtcggcgac
      481 cacccgggtc gtccagtcgg ggtcctcgac ggcaccgaga tcctcggcgg ccatcagccc
      541 gtagacattg cgctcccggg ccagctgccc gagtatcgtc tccgaggccg gccgcgcgcc
      601 ctccgcggcc agggcgcgcg cgagccagta gcgccacgag ggctcgcgcg actccgctgg
      661 cgacatgagg ttcacggcct cgcgtaccga cgaccagtta ccggcgcgca gcgcggcgcg
      721 cgccttccac tgcagctgcg cgtccgtgaa cggcccctcg ccagcctcgg cataccattc
      781 aagcgcgcgc ccgtggtgct gctgcgcggc cgcatgcgca acctgcgccc agccataacg
      841 cgcgtcgtcc ggccccagcg cgccgatccg gctcttcagc tgcgcggccg cgtcctcggg
      901 acgctttcgc gccaggcggg atacggcgaa aagaaggatc tcgcgcgtgg cccggctcgg
      961 gctgcgcgat ttctccgcag ccaggtagcg gtccggctcc gcctgggcgc gctcgagcgc
     1021 catttcgttc atctggtccg cggtggggag gaaggcattg gcgcgccggg cgtccttcac
     1081 gaggcccgcg gcgaggagct tgcggatgcg ctcccaggcc tcgggagggc cgatgaggcg
     1141 ttcgcgggcc gccgcctcga aggcgggggc gcaggctgca ggcgcctcgc gccctgcgag
     1201 ccacagggac cgcgcttccg cgggcgcatc ggcatcgttg cgcgcgaggc gttcctggag
     1261 cgagtagcag gcgacctcga cgtcgtcgct ggccaggccg gggaattcct ttcggaagag
     1321 gtcccactgg ccggcggctc caagcagctt cagccagtcg cggcgaagct ggtccgcgag
     1381 cggtccgtcg gcgcgcttcg cgagaaacgc gcggatgtcg tcgggcgggg cggtctccag
     1441 ggtggccgag agcagccagt attccggata cgacgcgagc aaatgcccgg cggtgcgggt
     1501 gcgcaggctc tcgagcgcct tcacgttgcc gcgcctggcc gcatcgcgcg cggccaggat
     1561 gtccgcgtcg gacgcgccgg ccgtcgcggt cagcgcgagg caggcgagcg cgagcagaca
     1621 gttcttgaac aaggtcaacg ccacggggat cccgatcgcc taatctcgcg gccattatag
     1681 aggctcggcg cggtgattcc cgcacggtgc gcaggagcgg atcggcgatc ggttaccatt
     1741 gaacaggtcc tggcgtgata ggggccgcac tcggaggaga gggaagcatg ggcgaggtga
     1801 tgacggcgca gttctggggc gggctgatgg cgatcatcgg cgtgaacatc gtcctttcgg
     1861 gcgacaacgc ggtcgtgatc gcgctcgccg cgcgcggact tcccccgaaa cagcagaagg
     1921 cggcgatctt ctggggcagc agcgcggcga tcgtcatgcg catcatcctc acgatcgtcg
     1981 cggtggaact gctgcgcctg ccctacctca agctcgtggg cgcggcgctc cttctctgga
     2041 tcgcggtgca gctgctgctg cccgaacacg attccgacga tggcgcgccg acctccggca
     2101 gcctgtgggc ggccatccgg acgatcctca tcgccgatct cgtgatgagc ctcgacaacg
     2161 tcatcggggt cgcggccgcg gcgaagggca gcgtcacgct gctcgtcctc ggtctcgcga
     2221 tcagcatccc gctcgtgatc ttcggcagca ccctcctcct gaagctcatg gagcgctatc
     2281 cggtcatcat caccggcggg gcggcgctgc tgggctgggt ggcgggcgac atggcggtga
     2341 cggatccgtc catcaaggac tggatcgacg ccaacatgtc gtggctgcac acggtggctc
     2401 ccgccgcggg ggccatcgcg gtcgtcgccg tcggcaggtg gcttgcggca cgcgcggagg
     2461 ccgcggagga gtccgagccg gcggtcgacc tggccaaggc cgaccggtcc aacggcggcg
     2521 ccggtccctg agagcattcg gcgaaccgaa aaaactgcct gatcacggga gaacgagatg
     2581 ttgcgcattc tggtcgcggt cgacggctcc gagtcttcgg accgcgccgt tgcacacctg
     2641 ctgaagaagc tgggatggta caaggagaag atcgaggtcc acctgctcaa cgtccaggcg
     2701 gcgctgccga gccaggtggg gctctacgcc ccggcggccg aacagctgca gggtttccac
     2761 cgcgagcagg gggaggcggc gctcgcgtcc gcccgggcga tcctcgatgg ggccaaggtc
     2821 gggtacgtga cccacatcgg catcggggag ccggcccacg tgatcgcgca ctacgcgaag
     2881 gaaaaatcgg tcgaccagat cgtcatgggc gcgcgcggcc tcggctcgat cgcggggctg
     2941 gtgatggggt ccgtgaccac gaaggtgctg ggcctcaccg acgttcccgt cctggtgatc
     3001 aagtagccga gaggatcccg cgccggggcg cccgccgggc ttgcggcccg agcgtgtccg
     3061 cgattgaccc gggtcaagag acgcgcgccc cccggcggct ctagaatgca ttcgtcgctg
     3121 cttgagcagg ttcggggcgg gaagcgggtt tcgcttgtcc gcggcctttc tccgcaagtt
     3181 agaatccggc aggccgttac tccatacggc cgcataacga ggagatactc aatgaaacaa
     3241 cgtcctttcg cgaagggcct gctggccgcc gtggtcgcca gcgcgttttc cgccctcacc
     3301 cccgccctgg cgtgggagcc caccaagacc gtcgaattca tcgtgcccgc gggcaccggc
     3361 ggcggcgcgg accagatggc gcgcctgtcg cagtcgatca tcgccaagca caacctcatg
     3421 aagcagtcgc tggtggtggt gaacaagggc ggcggcgccg gcgccgaggg cttcctcgac
     3481 gtcaagggcg ccaagggcga cccgcacaag atcatcatca cgctctccaa cctgttcacg
     3541 acgccgctcg ccacgggcgt gccgttcaac tggaaggacc tcacgccggt gcgcatgctg
     3601 gccctcgacc agttcgtcct gtgggtgaac aaggacgacc cggccaaga
//
LOCUS       DASUEV010000303         5092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1589479, whole genome shotgun sequence.
ACCESSION   DASUEV010000303 DASUEV010000000
VERSION     DASUEV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589479"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..759)
                     /locus_tag="VFV55_09565"
     CDS             complement(130..759)
                     /locus_tag="VFV55_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX4944587.1"
                     /translation="MNLLSAGPRPRETVPIFPLSTVLYPGTRLPLRIFEQRYMEMAKA
                     CLKHDSAFGVCLIREGEEVGTPAVPEAVGCLARIAEWDMETVGILKVKVDGLERFRLL
                     ATQTTPSGLILGDIEHIAAEDPVQAESPVQAAELEQCATFVRKVVDALGSARFTQPCR
                     YDDASWVGFRLAEILPLRNDVKQKLLELDDPGARLAVLHRFLLERDLVD"
     gene            complement(782..1621)
                     /gene="rapZ"
                     /locus_tag="VFV55_09570"
     CDS             complement(782..1621)
                     /gene="rapZ"
                     /locus_tag="VFV55_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX4944588.1"
                     /translation="MQLTLLSGVSGSGKSVALKALEDSGYFCVDNIPPNLIRGLVSYV
                     ASHGEPRVAVSADARSADTIGILPQIVEEERANGIDVRLVFLDATDASIVRRFAETRR
                     PHPLAGEGRTLEEALAIERALLAPIAEMGHRVDTSSLTPVQLRGWIHDLLVTDPTRLA
                     LCLESFGFKGGVPLNADFVFDARFLPNPYYDPQLRPKSGLDPEVAEFLERETEAGLLL
                     EDIHRFVVRWLPRFTLDRRAALTIAIGCTGGRHRSVYLVEKLAERLRPHHPLIVRHRD
                     LGE"
     gene            complement(1621..2589)
                     /gene="hprK"
                     /locus_tag="VFV55_09575"
     CDS             complement(1621..2589)
                     /gene="hprK"
                     /locus_tag="VFV55_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX4944589.1"
                     /translation="MPRLSVQQLFEDRRERLGLAWAAGASGSHRELTNEMLSRPGLGL
                     IGHLNLIHPLIVQVLGSSELDYISRMSETARTEMMERLAGGDTLCIVVGDSLPVPPAL
                     ERAATRAAVPLLVARESSQQVINVLRPYLQGELGEVTSLHGVFLDVLEIGVLITGESS
                     IGKSELALELISRGHGLVADDVVELQQIGPETVQGRCPPMLRDFLEVRGLGVLNIRSI
                     FGETAVRPRKALRLIVHLEFPADGLYEERDRLATRSGTRRVLGVEIPTVTLTVAPGRN
                     LAVLVEAAVRNHILITRGIDSTRDFIARQAAAMRGRDPGDREPRGD"
     gene            complement(2558..3043)
                     /locus_tag="VFV55_09580"
     CDS             complement(2558..3043)
                     /locus_tag="VFV55_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX4944590.1"
                     /translation="MNRLSPMLPAAHVLLDLDVGSKKRLFEQIGLLFENSRQIPRARV
                     FDSLFDREKLGSTGLGYGVAIPHGRLKNLKETLCAFVRTQSPVPFESPDGQPVRLVLA
                     MLVPEHATEQHLELLSDLAQMFSDRGLRERLLAAGDIDAAHRLLTEWSPYAPAQRSAA
                     L"
     gene            complement(3075..3395)
                     /gene="raiA"
                     /locus_tag="VFV55_09585"
     CDS             complement(3075..3395)
                     /gene="raiA"
                     /locus_tag="VFV55_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX4944591.1"
                     /translation="MNLQLTGHHVEITPSIREYVVSKLERINRHFEQVIDVNVILSVQ
                     KLDQMIEATVHLSGKDIHVECHDADMYAAIDGLVDKLDRQIIRHKEKFQEGRHDAGHK
                     RTAT"
     gene            complement(3419..4849)
                     /locus_tag="VFV55_09590"
     CDS             complement(3419..4849)
                     /locus_tag="VFV55_09590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX4944592.1"
                     /translation="MKHSLQLKLSQHLTLTPQLQQSIRLLQMSTVELNQELERYLTEN
                     PLLERADLAGDEAPIGPPRDPAPNGDAAPASEPQSEASTQPDDMISFGEESYGGSRRN
                     EDGDDDFTQIAAAEPSLQEALIRQLQLTSMSERDKRLALLVIANLDGDGYLAATMEEL
                     FEIGADELEDLEVEEISIALRQVQNLEPAGVGARNLGECLALQLKTMDPDTPFRDCAI
                     ELVSHHLDLLAARDFNKLRRLLGVTEDELREVQALILTLDPKPGHAWGEGDVRYVVPD
                     VTVKKTGGRWLAQLNRDAMPRLRVNKMYADLLQSSRDNGGRNLAGQLQEARWLIKNVQ
                     QRFDTILRVTQAIVDRQRNFFEHGEVAMRPLVLREVADAVGLHESTISRVTTQKYMLT
                     TRGIFELKYFFGSHVATDAGGACSSTAIRALIKQLVAAEDGKKPLSDHRISDILAQQG
                     IQVARRTVAKYREAMHIRPANMRKSI"
     gene            complement(4883..>5092)
                     /locus_tag="VFV55_09595"
     CDS             complement(4883..>5092)
                     /locus_tag="VFV55_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX4944593.1"
                     /translation="VLDIQQIIRFLKERGIGVLITDHNVRETLGICDHAYIINQGSVL
                     AFGKPEEIIYNEAVRKVYLGENFRL"
BASE COUNT          774 a         1629 c         1820 g          869 t
ORIGIN      
        1 ggtgagcgtg aagggcgtgg cgcccgcgag cctcgggccg tccgccatgt ggcttgccct
       61 gcaggaggtg cccgaggcag cccttccggc gccggtgaag aaactgctcg cgcgcctcgc
      121 gcgcggcgcc tagtcgacga ggtcgcgctc gagcaggaag cggtgcagga ccgcgaggcg
      181 ggcgccggga tcgtcgagct ccagcagctt ctgcttcacg tcgttgcgca gcggcaggat
      241 ctcggcgagg cggaagccga cccagctcgc gtcgtcgtag cggcagggct gcgtgaagcg
      301 cgccgatccg agcgcatcga cgaccttgcg cacgaaggtg gcgcactgct ccagctcggc
      361 cgcctgcacg ggagactccg cctgcacggg atcctccgcg gcgatgtgct cgatgtcgcc
      421 gaggatcaac ccggaaggcg tggtctgcgt cgcgagcagc cggaaccgct cgagcccgtc
      481 gaccttgacc ttgaggatgc cgaccgtttc catgtcccat tcggcgatgc gcgcgaggca
      541 gcccaccgcc tctgggacag cgggcgttcc cacctcttcg ccttcgcgga tcaggcacac
      601 gccgaaggcg gagtcgtgct tgaggcaggc cttggccatt tccatgtagc gctgctcgaa
      661 gatccggagt ggcaggcgcg ttcccggata gagcacggtc gacagcggga atatgggcac
      721 cgtctcgcgc ggcctgggcc ccgcggacag gaggttcatg acgagcacga ccggcgcttc
      781 cctattcccc gagatcgcgg tgacgcacga tgagcggatg gtgcgggcga agtcgctcgg
      841 cgagtttctc gacgagatac accgagcggt ggcgcccgcc ggtgcagccg atcgcaatgg
      901 taagggccgc gcggcggtcg agcgtgaatc gcgggagcca gcgcacgacg aaccggtgga
      961 tgtcctcgag gagcaggccg gcttccgtct cgcgctcgag gaactccgcc acctccggat
     1021 cgaggcccga tttcggccgg agctgcgggt cgtagtacgg gttgggcagg aagcgcgcgt
     1081 cgaagacgaa atccgcgttc agcggcacgc cgcccttgaa cccgaacgac tccaggcaca
     1141 gtgccagccg ggtggggtcg gtcacgagga gatcgtgaat ccatccgcgc agctgcaccg
     1201 gggtgaggga actcgtgtcg acgcgatggc ccatttccgc gatcggcgca aggagggcgc
     1261 gctcgatcgc gagtgcctcc tccagggtgc ggccttcccc ggctagcggg tgcgggcggc
     1321 gcgtctcggc aaagcgccgc acgatgctcg cgtcggtcgc gtcgagaaaa acgaggcgga
     1381 catcgatgcc gttcgcgcgt tcctcttcca cgatctgcgg caggatgccg atcgtgtccg
     1441 cgctacgggc gtccgcgctc accgccacgc ggggttcgcc gtgggaagcc acgtaggaca
     1501 ccagcccgcg gatgaggttg ggcgggatgt tgtccacgca gaaatagccc gagtcctcga
     1561 gcgccttgag cgcgacgctc ttgccggacc cggaaactcc gctgaggagc gtgagctgca
     1621 tcagtcgccg cgcggctcgc ggtccccggg atcgcgcccg cgcatcgcgg cagcctggcg
     1681 ggcgatgaaa tcgcgcgtgg aatcgatgcc gcgcgtgatc aggatgtggt tgcgcaccgc
     1741 agcctcgacc agcaccgcga ggttgcgccc cggcgcgacg gtcagcgtca ccgtcgggat
     1801 ctcgacgccc agcacccgcc tcgtgcccga acgcgtcgcg aggcgatcgc gttcctcgta
     1861 taggccgtcg gcggggaact cgaggtggac gatcaggcgc agcgccttgc gcggccgaac
     1921 cgccgtctcg ccgaagatcg accggatgtt gagcacgccc aggcccctca cctcgagaaa
     1981 gtcgcgcagc atcggcgggc agcggccctg caccgtctcc ggcccgatct gctgcagctc
     2041 caccacgtcg tcggcgacga gtccgtggcc gcgcgagatg agctccaggg cgagctcgct
     2101 cttgccgatg gaggattcgc ccgtgatgag gaccccgatc tcgagcacgt cgaggaagac
     2161 gccgtgcagc gacgtcacct cgcccagctc gccctgcagg tacgggcgca ggacgtttat
     2221 cacctgctgg ctcgactcgc gcgccacgag gagggggact gcggcccggg tcgcggcacg
     2281 ctccagcgcg ggcggaacgg gaaggctgtc gcccaccacg atgcacagcg tgtcgccccc
     2341 ggccaggcgc tccatcatct cggtgcgcgc ggtctcgctc atgcgcgaga tgtagtcgag
     2401 ctcgctcgag ccgagcacct gcacgatgag cgggtggatg aggttcaggt gcccgatgag
     2461 ccccaggccc gggcgcgaga gcatctcgtt ggtgagctcc cggtggcttc ccgaggcgcc
     2521 cgccgcccag gcgagcccca ggcgttcgcg ccggtcctca aagagctgct gaacgctgag
     2581 ccggggcata cggcgaccac tccgtgagca ggcggtgggc cgcatcgata tcgccggcgg
     2641 ccaggagacg ctcgcgaagc ccgcggtcgc tgaacatctg ggcgaggtcg gacaggagct
     2701 ccaggtgctg ttcggtcgcg tgctcgggca cgagcatcgc caggacaagc cgcaccggct
     2761 ggccgtccgg cgactcgaag ggcacggggg attgcgtgcg cacgaaggcg cagagcgttt
     2821 ccttgaggtt cttcagccgg ccgtgcggga tcgcaacgcc gtagcccaat ccggtggagc
     2881 ccagcttttc gcggtcgaag aggctgtcga agacgcgcgc gcgcgggatc tgcctcgagt
     2941 tctcgaacag cagtccgatc tgctcgaaga ggcgcttctt gctgcccacg tcgaggtcga
     3001 gcaggacgtg cgcggcaggc aacatgggcg agagcctgtt catggcggtg tttccggggg
     3061 gctggccctg cagctcacgt ggcggtgcgt ttgtggcctg catcatggcg tccctcctgg
     3121 aacttctcct tgtgccggat gatctgccgg tcgagcttgt ccaccaggcc gtcgatcgcc
     3181 gcgtacatgt cggcgtcgtg gcactccacg tggatgtcct tgccggaaag gtggacggtc
     3241 gcctcgatca tctggtcgag cttctgcacc gacaggataa cgttcacgtc gatgacctgt
     3301 tcgaaatgcc ggttgatccg ctcgagcttg gagacgacgt attcgcggat ggacggggtg
     3361 atttcgacat ggtggccggt gagctggagg ttcatcgagt gctcctttgc gttttcattc
     3421 agatggactt tctcatgttc gcggggcgga tgtgcatcgc ctcgcgatac ttcgccaccg
     3481 tccttcgcgc gacctggatg ccctgctgcg cgaggatgtc cgagatgcgg tggtcggaaa
     3541 ggggcttctt gccgtcctcg gcggccacga gctgcttgat gagcgcgcgg atggcggtgg
     3601 aggagcatgc gccgccggcg tcggtcgcga cgtggctgcc gaagaagtac ttgagctcga
     3661 agatgccgcg cgtcgtgagc atgtatttct gcgtggtcac ccgcgagatc gtcgattcgt
     3721 gcagcccgac ggcgtcggcg acctcgcgca gcacgagcgg gcgcatcgcg acctcgccgt
     3781 gctcgaagaa gttccgctgg cggtcgacga tcgcctgcgt cacgcgcagg atggtgtcga
     3841 agcgctgctg cacgttcttg atcagccagc gtgcctcctg gagctggccc gcgaggttcc
     3901 tgccgccgtt gtcgcgcgag gattgcagga ggtcggcgta catcttgttc acgcgcaggc
     3961 gcggcatcgc gtcgcggttc agctgcgcga gccaccggcc gccggtcttc ttcaccgtga
     4021 cgtcggggac gacgtagcgc acgtcgccct cgccccacgc gtggccgggc ttgggatcga
     4081 gcgtgaggat gagggcctgc acttcgcgca attcgtcttc cgtgacgccg agcaggcggc
     4141 gcagcttgtt gaagtcgcgg gcggcgagca ggtcgagatg atgcgagacg agctcgatcg
     4201 cgcagtcgcg gaagggcgtg tcgggatcca tcgtcttcag ctgcagcgcg aggcactcgc
     4261 cgaggttcct cgcgccgacg ccggccggct cgaggttctg cacctgccgc agggcgatgg
     4321 agatctcctc gacctcgagg tcctcgagct cgtcggcgcc gatctcgaaa agctcctcca
     4381 tggtggccgc gagatacccg tcgccgtcca ggttcgcgat cacgagcagg gccaggcgct
     4441 tgtcgcgctc gctcatcgag gtgagctgca gttgccggat cagcgcctcc tgcaggctgg
     4501 gctccgcggc ggcgatctgc gtgaaatcgt cgtcgccgtc ctcgttgcgc cgcgagccac
     4561 cgtaggactc ttcaccgaag gagatcatgt cgtccggttg cgtggacgcc tcggactgcg
     4621 gctcggaggc tggagcggcg tcgccgttgg gcgcggggtc ccgggggggg ccgatcggcg
     4681 cctcgtcgcc ggcgaggtcg gcgcgctcga ggagcggatt ttccgtgagg tagcgctcga
     4741 gctcctgatt cagctcgacc gtcgacatct gcagcaaccg gatggattgc tgcagctgcg
     4801 gcgtgagcgt gagatgctgg gatagcttga gctggaggct gtgcttcatg tcgaaagccg
     4861 cggggtgaat tatgggagcc cgctagaggc ggaagttctc cccgaggtag acctttcgga
     4921 cggcttcatt atagatgatt tcctccggct tcccgaaggc gaggaccgag ccctggttga
     4981 tgatgtaggc atggtcgcag atgcccagcg tctcccgtac gttgtggtcg gtgatgagca
     5041 cgccgatgcc gcgctccttg aggaagcgga tgatctgctg gatgtccagg ac
//
LOCUS       DASUEV010000304         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_900960, whole genome shotgun sequence.
ACCESSION   DASUEV010000304 DASUEV010000000
VERSION     DASUEV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900960"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFV55_09600"
     CDS             <1..819
                     /locus_tag="VFV55_09600"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HEX4944594.1"
                     /translation="GTDRWYTSGVRIYRSALLDEVSPFASFLRGCATREQRFDVGILH
                     EVYTGDGRADPTAPDRPNAGRLLLSFARHDLSPDTLASLGIDAGVSGPAALGERVQEI
                     IHRIAPAPHTDWTRQVDNRADVQVTGAWSRRLMADAIPGALVMHGGAAAGTITAFGHA
                     GIEWRTDSPAEAANPLLRFAATPPMPRNARGLSLFAGASLRAIARNRVLGRRSDDPKP
                     EAAREHRVSRVAAGLAWSEDWGVVTLGLAQDSREFDGQRSPHRFGSLTVSFPAD"
     gene            828..2462
                     /locus_tag="VFV55_09605"
     CDS             828..2462
                     /locus_tag="VFV55_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4944595.1"
                     /translation="MQVAFVHGIRFRLLLASLVLLAIPALAAQFINRMETFLRAAQEQ
                     DIAVTARAVSSALSDRPALFPPGDSAPGDPDDEERRRIVALFAAADTIAAASLGSAYA
                     PSEEIERFLDILGRRASRLWVVDTRSRVRGLSGTLREPPPSRANASRTAVAGWLKPVV
                     SLVVSSPEVPAGDESKPVRAQIDRALIGVSSTYWRGTKDREVAILSAAQPVFVGDDIV
                     GAVVVEETTASILLLRQSALENLIALTLAVCLAAFAILLFFASRLATRIRRLHAEAES
                     AIDAQGRIRGAITPTGAKDEIGDLERTISAVLGRLRDYNAYLEQMAGRLSHELRTPVA
                     VVRSSLDNLRSQSPGGEARVYLDRADVGVDRLSRLISRLSEGTRLEKMLESAERESFD
                     LAAVVRGCVEGYRTAYPGRAFECELPAAEVMINGVPDALAQLLDKLVENALDFAPAGS
                     AIRIGLSCDGRLARLGVANDGPPIPAAMLPRLFDAMVSAREEGHGRGGHLGLGLAIVR
                     LVAEFHGGTARAANLADGRGVYFEVELAAQGPRHPA"
     gene            2502..2726
                     /locus_tag="VFV55_09610"
     CDS             2502..2726
                     /locus_tag="VFV55_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX4944596.1"
                     /translation="MARILLLLAIGFFIWLLFRGFFRSQVNDASRSEPRDVEDMVACA
                     RCGVNMPRSESKEEGGRITCRDPSTCKHSR"
     gene            2723..4357
                     /locus_tag="VFV55_09615"
     CDS             2723..4357
                     /locus_tag="VFV55_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4944597.1"
                     /translation="MTPAALATGALAERPRPGERAPMAAAGDNAAWQTLVVFSAYRVV
                     LALGFSGAYAFLNSIFQLGIQERALAVIALAAYMVASVLLVIPALLREPGISLQVTVA
                     VFVDVLCIGVLMHTSGGIRSGLGVSLLISLAAAGLITRGRLAFFHAAVAAIAVLLEQA
                     FQVWRFDAPVSDFLQSGLTSMALFATAGLGWKLAGYARTSETIARQRTIDLANLSQIN
                     ELVIRDMQDGMLVVDPEGTIRQSNPRAAQLLGPFPSVRRPLLAEYSPEIARLMESWRR
                     GPETTFLQFRAPRSAADLAVRFIAIGSGDPPPTVIFVEDVGRLRGQAQQMKLVALGRL
                     TASIAHEIRNPLSSINHAAELLAESEHAQEGDDRLVAIIRDNVHRLDRIVQEVLYLNR
                     RDRAQPEAIESRAFLEQFVVDFRSNERVPADGVQVDVRTALRMSFDRQHLHQVLWNLC
                     RNAWRHGLKLRGSVRISLAPADAPGMLALEVADDGPGVAEEVQAHLFEPFFTTEPQGT
                     GLGLYVARELCEGNGARIEYREGGPGGRFRVTLKGT"
BASE COUNT          583 a         1643 c         1470 g          698 t
ORIGIN      
        1 ggcaccgacc gttggtacac gagcggcgtg cgcatctacc gctcggcgct cctggacgag
       61 gtctctcctt tcgcgtcctt cctgcgtggc tgcgcaacgc gcgagcagcg cttcgacgtc
      121 ggaatccttc acgaggtcta caccggcgac gggcgcgccg acccgaccgc acccgaccgc
      181 ccgaacgcgg gccgcctcct gctctccttc gcgcgccacg acctctcgcc tgacacgctg
      241 gcctccctgg ggatcgacgc gggcgtctcc ggtcccgcgg cgctgggcga gagggttcag
      301 gaaatcatcc atcgcatcgc ccccgcgccg cacacggact ggacgaggca ggtggacaac
      361 cgcgccgatg tgcaggtgac cggtgcctgg tcgcgccgct tgatggccga tgccattccc
      421 ggcgcgctcg tgatgcacgg cggtgcggcg gcgggcacga tcaccgcgtt cggccacgcc
      481 ggcatcgaat ggcgtacgga cagcccggcc gaggccgcca acccgttgct tcgcttcgcc
      541 gcgacgccgc cgatgccgcg caatgcgcgc ggcctctcgc ttttcgccgg cgcgagcctg
      601 cgcgccatcg cgcgcaaccg cgtcctcggg cgccgttccg atgacccgaa gcccgaggcg
      661 gcccgggagc atcgcgtgag ccgggtggcc gcgggactcg cctggtccga ggactggggc
      721 gtggtcaccc tgggcctcgc gcaggactcc cgcgaattcg acggccagcg atcgccgcac
      781 cgtttcggct cgctcacggt ctccttcccg gccgactgag cgtgacgatg caagtggcct
      841 tcgttcacgg catccgcttc cggctgctcc tcgcctcgct ggtactgctg gccattcccg
      901 cgctcgccgc gcagttcatc aaccgcatgg agactttcct gcgcgccgcc caggagcagg
      961 atatcgccgt caccgcgcgc gccgtgtcct cggccctttc cgaccgcccc gcgctcttcc
     1021 ctcccggcga cagcgcgccc ggcgatccgg acgacgagga acgccgccgc atcgtcgcgc
     1081 tcttcgccgc cgcggacacg atcgccgcgg caagcctggg atccgcctat gcaccgtccg
     1141 aggagatcga gcgcttcctc gacatcctgg gccgccgggc ttcgcgcctt tgggtggtgg
     1201 acacgcgctc gcgcgtgcgc ggcctgtcgg gaacgctgcg cgagccgccg ccatcgcgcg
     1261 ccaatgcctc gcgaaccgcg gtggccgggt ggctcaagcc cgtcgtttcc ctcgtggtgt
     1321 cctcgcccga agtccccgcg ggcgacgaat cgaagccggt gcgcgcgcag atcgaccgcg
     1381 ccctcatcgg cgtctcctcc acctactggc gcgggacgaa ggaccgcgag gtggcgatcc
     1441 tgtccgccgc ccagccggtg ttcgtgggcg acgacatcgt gggcgccgtg gtggtcgagg
     1501 agacgaccgc ctccatcctc ctgctgaggc agtcggcgct cgagaacctc atcgcgttga
     1561 cgctcgcggt ctgcctggcc gcgttcgcga tcctcctctt cttcgccagt cgcctggcca
     1621 cgcgcatccg ccgcctgcac gccgaggcgg agtccgccat cgacgcgcag gggcgcatcc
     1681 gcggcgccat cacgcccacc ggggcgaagg acgagatcgg cgacctggag cgcacgattt
     1741 ccgcggttct cgggcggctt cgcgactaca acgcctacct cgagcagatg gcggggcgcc
     1801 tttcccacga attgcgcacg cccgtcgccg ttgtgcgctc ctcgctcgac aacctgcgca
     1861 gccagtcgcc cgggggggaa gcgcgcgtct acctcgatcg cgcggatgtc ggcgtggacc
     1921 ggctctcgcg gctcatctcg cgcctttcgg aaggaacgcg actggaaaag atgctggaaa
     1981 gcgcggagcg cgaatccttc gatctcgccg ccgtggtgcg cggctgcgtc gagggctacc
     2041 gtaccgccta tcccggccgc gccttcgagt gcgagttgcc ggcagccgag gtgatgatca
     2101 acggcgtgcc cgatgcgctc gcgcagctcc tcgacaagct ggtcgagaac gcgctcgact
     2161 tcgcgcccgc cggcagcgcc atccggatcg ggctctcgtg cgacggccgg ctcgcacgcc
     2221 tgggcgtggc gaacgacggc ccgcccattc cggccgcgat gttgccgcgc ctcttcgatg
     2281 ccatggtgtc ggcgcgcgag gagggccacg gccgcggcgg ccacctcggg ctgggcctcg
     2341 ccatcgtccg cctcgtggcc gaattccacg gcggcaccgc ccgggcggcc aacctggcgg
     2401 acggcagggg cgtgtacttc gaggtggaat tggccgcgca gggtccccgg caccccgcgt
     2461 gagctaaact tgggacaggg aatccgtccg gaatcacgac cttggccaga atacttctcc
     2521 tgctcgcgat cggcttcttc atctggctgc tgttccgcgg cttcttcagg tcgcaggtga
     2581 atgacgcgag ccgctcggag ccgcgcgacg tcgaggacat ggtcgcctgc gcgcgctgcg
     2641 gcgtgaacat gccgcgcagc gagtcgaagg aagaaggcgg caggatcacc tgccgcgacc
     2701 cctccacctg caagcactcg cgatgacgcc tgccgcgctc gccaccgggg ccctcgccga
     2761 acgaccgcga cccggcgagc gcgcccccat ggccgcggcc ggggacaacg cggcctggca
     2821 gacgctcgtc gtcttttcgg cctaccgcgt cgttctggcc ctggggttct ccggcgccta
     2881 cgcgttcctc aattcgattt tccagcttgg catccaggag cgggcgctgg cggtgatcgc
     2941 gctggcggcc tacatggtgg cctcggtgct tctggtcatt cccgcgctcc ttcgcgagcc
     3001 gggcatctcg ctgcaggtca cggtggcggt cttcgtcgac gtgctgtgca tcggcgtgct
     3061 catgcacacg agcggcggca tccgctcggg gctcggcgtg tcgctgctca tctcgctcgc
     3121 cgcggcgggc ctgatcacgc gcggccgcct ggcgttcttc cacgcggccg tcgcggccat
     3181 cgccgtgctg ctcgagcagg cgttccaggt gtggcgcttc gacgcgccgg tgagcgactt
     3241 cctgcaatcg ggcctcacca gcatggcgct cttcgccacg gcggggctgg gctggaagct
     3301 cgccggctac gcgcgcacca gcgagaccat cgcccggcag cgcaccatcg acctcgccaa
     3361 cctctcgcag atcaacgagc tggtcatccg cgacatgcag gacggcatgc tcgtggtcga
     3421 tccggaaggc accatccgcc agagcaaccc gcgcgcggcg cagctcctcg ggccctttcc
     3481 atcggtgcgg cgccccttgc tcgccgagta ttccccggag atcgcgcgcc tgatggaatc
     3541 ctggcggcgc gggccggaga ccacgtttct ccagttccgc gcgccgcgct ccgccgccga
     3601 ccttgccgtg cgcttcattg ccatcggatc cggggaccct ccccccaccg tgatattcgt
     3661 cgaggacgtg gggcggcttc gcggtcaggc ccagcagatg aagctcgtgg cgctggggcg
     3721 gctcaccgcc tcgatcgccc acgagatcag gaacccgctt tcctcgatca accacgccgc
     3781 ggaactgctg gccgagagcg agcatgcgca ggagggcgac gaccggctcg tcgccatcat
     3841 ccgcgacaac gtccatcgcc tcgaccgcat cgtgcaggag gttctctacc tcaatcgccg
     3901 cgaccgcgcg cagcccgagg cgatcgagtc gcgcgcgttc ctcgagcagt tcgtcgtcga
     3961 cttccgttcc aacgagcggg tgcccgcgga cggcgtgcag gtggacgtgc gcacggcgct
     4021 gcgcatgagc ttcgaccgcc agcacctgca ccaggtcctc tggaacctgt gccgcaacgc
     4081 ctggcgccac ggcctcaagc tgcgcggctc ggtgcgcatc tcgcttgcgc ccgcggacgc
     4141 cccgggcatg ctcgcgctcg aggtggcgga tgacggcccg ggcgtggcgg aggaggtgca
     4201 ggcgcacctc ttcgagccct tcttcaccac cgagccgcag ggcacgggac tcggcctcta
     4261 tgtcgcgcgc gaactctgcg aaggcaacgg cgcgcgcatc gaataccgcg agggcggccc
     4321 cggcggccgc ttccgcgtca ccctgaaagg cacctgatga gcgccaaggc cgtcctgatc
     4381 gtcgacgacg aggc
//
LOCUS       DASUEV010000305         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1592722, whole genome shotgun sequence.
ACCESSION   DASUEV010000305 DASUEV010000000
VERSION     DASUEV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592722"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFV55_09620"
     CDS             <1..306
                     /locus_tag="VFV55_09620"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX4944598.1"
                     /translation="QSGGIGMGVSLLSYNGCIQFGLITDAGLVPDPDRIVARFHDEFE
                     KLMWLTLMVPWEGEELPGPPSGDAATEEPRRPSTGRGKKAKEAAKQPMHIPKRFRNL"
     gene            complement(474..866)
                     /locus_tag="VFV55_09625"
     CDS             complement(474..866)
                     /locus_tag="VFV55_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX4944599.1"
                     /translation="MAAKKAKKPAAKKPAAKKPAAKKPAAKKPAAKKPAAKKAAPKKP
                     AVKRKPNAAFMKPLMPSAALAAVIGPAAQPRTEVTSKLWAYIKKNNLQDKVNRRNINA
                     DDKLREVFGGKKTVSMFEMTKLVSKHLK"
     gene            complement(1041..1850)
                     /locus_tag="VFV55_09630"
     CDS             complement(1041..1850)
                     /locus_tag="VFV55_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944600.1"
                     /translation="MATSNSEEEAMAKAKWSPFPYPDKAYTYAGDALKKNWDRLHRGD
                     CEPFPKDPKVQDAWRQYHRGAFAEAVALGLAAGGAGVTVANKAQSIYANGLEKKQAVR
                     MVLFEEAMKRAEAQAAAEPKNANAHYQYAYAAGRYSQGISVVNALAQGFGGRIRAALE
                     ATLKLAPRHAEAHIALGTYHAEIIDKVGALVGGLTYGAKKDAGEEHFRKALKLTPDSP
                     IALIEFANGLVMMHGRKALDEASSLYAKAASLEPHDAMEKIDAEAAKAELE"
     gene            complement(1834..2514)
                     /locus_tag="VFV55_09635"
     CDS             complement(1834..2514)
                     /locus_tag="VFV55_09635"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4944601.1"
                     /translation="MAKATAGRPALDGNAVRTQRTRERILAVSLGLFNDRGEANVTTG
                     MIADELNISPGNLYYHFRNKDQIVERLFDRFEERISIAPPEPMAGAGAIEDLWLYLHL
                     MFEAIWDYRFLYRNLDDIVGRNRRLREHFGRIVETKLGAVEGLCRGLADSGLMNANAG
                     EIRALSLNVLVVATYWLNFRAVRNDRESPDPADPGTGAFQVMSLVAPYLAGEARRHFQ
                     RLGRGYIE"
     gene            complement(2529..>3610)
                     /locus_tag="VFV55_09640"
     CDS             complement(2529..>3610)
                     /locus_tag="VFV55_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX4944602.1"
                     /translation="SFLAAALANGIPISEIYGIFIEGEGAKGALTPEVFMRPAFREYL
                     QRARTVPPILVRSLWQYMRQPFSRGALESFAALGRAIPTGVFDNDTIHRHLHAVFTQP
                     GRTDDFRELRHRLYLVATDLDSGESVSFGRPGHDHVPISQAVQASAALPGLFPPVEID
                     GRSFVDGALKKTLHASEALDDGAKLVICVNPLVPYDASLAAEKGRGRHRRLVEGGLPV
                     VLSQTFRAIIHSRMAVGISKYRNAYRDADVVLFEPDADDSEIFFTNIFSYSTRVRLCE
                     HAYLRTRRDLLRRRYELEPLLARHGVRLRIDVLGDANRRIGLRPNLRARSLPAAWRLY
                     DAAADLRDTLVDLRHWLAAKDRATA"
BASE COUNT          562 a         1167 c         1218 g          663 t
ORIGIN      
        1 caatcgggag gaatcggaat gggcgtttcg ctcctctcgt acaacggctg catccagttc
       61 ggcctcatca cggatgcagg cctcgtgccg gacccggacc gcatcgtggc gcggttccac
      121 gacgagttcg agaagctcat gtggctcacg ctgatggtcc cgtgggaagg cgaggaactg
      181 cccgggcctc cgtctggcga cgctgcaacg gaagaacccc ggcgcccgtc caccgggcgg
      241 ggcaagaaag cgaaggaagc tgcaaagcaa ccgatgcaca tcccgaagcg attcaggaac
      301 ctttgagatc cggggcggtg ggttcaagca caggggccac agaggacaca gagggcacag
      361 aggctgcgtt caaacccacc gcatcctgcc tctccgccga ccccgaataa gcaccaacaa
      421 aaacgccggg agcctttcgg cccccggcgt tcttgcgtac ttcgcagccc ggcttacttg
      481 aggtgcttgg agacgagctt ggtcatctcg aacatcgaga ccgtcttctt gccgccgaaa
      541 acctcgcgca gcttgtcgtc ggcgttgatg ttccgcctgt tgaccttgtc ctggaggttg
      601 ttcttcttga tgtaggccca cagtttgctg gtcacttcgg tgcggggctg ggcggccggg
      661 ccgatgacgg cggcgagcgc ggcgctcggc atgaggggct tcatgaaagc ggcattgggc
      721 ttgcgcttga cggccggctt cttgggcgca gccttctttg ccgccggctt cttggcggcg
      781 ggcttcttgg ccgccggctt cttcgccgcg ggtttcttgg ccgccggctt cttcgccgcg
      841 ggcttcttgg ctttcttcgc tgccattcgg aatcctccaa tcagattgtg acgtttggtt
      901 cagctgatct cgcctctaga ggcgtgacag gcgagagaat gcacacggca aaatcggttt
      961 gtcaatggat tttcactctg aaaacgcgct ttctccactc tgcgtcgtcg acgtgcaaca
     1021 cggcatttcg cggcgcgcgc tcattcgagc tctgccttcg ccgcttcggc gtcgatcttc
     1081 tccatggcgt catgcggctc gagggaagcg gccttcgcgt aaagggagga cgcctcgtcg
     1141 agcgctttcc tgccgtgcat catcaccagg ccgttggcga actcgatgag cgcgatcggc
     1201 gagtccggcg tgagcttgag cgccttgcgg aaatgctcct cgcccgcgtc cttcttcgcc
     1261 ccgtaggtga ggccgccgac aagcgcaccc accttgtcga tgatctcggc atggtaggtt
     1321 cccagcgcga tatgggcctc ggcatgccgg ggggccagct tgagcgtcgc ctcgagcgcg
     1381 gcgcgtatcc ggccaccgaa gccctgggcc agcgcattga ccaccgagat gccctggctg
     1441 taacggccgg cggcgtaggc gtactggtag tgcgcgttgg cgttcttcgg ctcggccgcg
     1501 gcctgggcct cggctcgctt catcgcctct tcgaagagaa ccatgcgcac cgcctgcttc
     1561 ttctccaggc cgttggcgta gatcgactgc gccttgtttg caaccgtgac gccggcgcct
     1621 ccggccgcca ggccgagcgc gaccgcctcg gcgaacgcgc cacggtggta ttggcgccac
     1681 gcatcctgga ccttcggatc cttcggaaac ggctcgcagt cgccgcgatg aaggcggtcc
     1741 cagttcttct tcagcgcgtc gcccgcatag gtgtatgcct tgtccgggta cggaaacggg
     1801 ctccatttcg ccttcgccat cgcctcctcc tcgctattcg atgtagccac ggccgaggcg
     1861 ctggaaatgc cgcctcgcct cgcccgccag gtacggcgcc accagcgaca tcacctggaa
     1921 tgcgccggtg cccggatcgg ccgggtcggg cgactcccga tcgttgcgga cggcgcggaa
     1981 attgagccag taggtggcga ccaccagcac gttgagggag agcgcccgga tttcgccggc
     2041 gttcgcgttc atcaggccgc tgtcggcaag accgcggcac aggccctcga ctgccccgag
     2101 cttcgtctcg acgatccggc cgaagtgctc gcgcaggcgg cggttgcggc cgacgatgtc
     2161 gtcgaggttg cggtagagga agcggtagtc ccagatggcc tcgaacatga ggtgcaggta
     2221 gagccacagg tcctcgatcg ccccggcgcc ggccatcggc tcgggcggcg cgatcgagat
     2281 gcgctcctcg aaacgatcga acagccgctc gacgatctgg tccttgttgc ggaagtggta
     2341 gtagaggttc cccgggctga tgttcagctc gtcggcgatc attccggtgg tgacgttcgc
     2401 ctcgccgcgg tcgttgaaga gcccgaggct cacggcaagg atgcgctccc gggtgcgctg
     2461 ggtgcgcacc gcattgccgt cgagggcggg ccggccagcg gtagctttcg ccatggccgg
     2521 agaggttatc acgccgtggc gcgatccttt gcggccagcc agtgccggag gtccacgagc
     2581 gtatcgcgca ggtcggccgc ggcgtcgtac agacgccatg cggcaggcag cgatcgcgcg
     2641 cgaaggttcg ggcgcagtcc gatgcggcgg ttcgcgtcgc cgaggacgtc gatccgcagc
     2701 cgcacgccgt ggcgggcgag cagcggctcg agttcgtagc gccgccgcag caggtcccgc
     2761 cgggtgcgca ggtaggcgtg ctcgcacagg cgaacccggg tcgagtagct gaagatgttg
     2821 gtgaagaaga tctcgctgtc gtccgcgtcc ggttcgaaca ggacgacgtc cgcgtcgcgg
     2881 tatgcgttgc ggtacttcga gatcccgact gccatccgcg agtggatgat ggcgcggaag
     2941 gtctgcgaaa gaaccaccgg gagcccgccc tcgacgaggc ggcggtggcg cccccttccc
     3001 ttctccgcgg cgaggctcgc gtcgtaggga acgagcggat tcacgcagat caccagtttc
     3061 gccccgtcgt cgagcgcttc cgaggcgtgc agcgtcttct tcagggcccc gtcgacgaag
     3121 ctgcgcccgt cgatctctac cggcgggaaa aggcccggca gcgcggcact tgcctggaca
     3181 gcctgcgaga tgggcacgtg gtcgtgcccg ggccgcccga agctcacgct ctcgccgctg
     3241 tccaggtcgg ttgccacgag atagagcctg tgcctgagct cacggaaatc gtcggtccgg
     3301 cccggctggg tgaacaccgc gtgcaggtgc cggtggatgg tgtcattgtc gaagacgccg
     3361 gtgggaatgg cccggccaag ggccgcaaac gactccagcg ccccgcgcga aaacggctgc
     3421 cgcatgtatt gccagagcga gcgcaccagg atcggcggca ccgttcgtgc acgctgcagg
     3481 tattcccgga aggcggggcg catgaacact tcgggcgtga gcgcgccctt cgccccttcg
     3541 ccctcgatga agatcccgta gatttccgag atggggatgc cgttggcgag cgccgcggcg
     3601 agaaagctgc
//
LOCUS       DASUEV010000306         6634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_308601, whole genome shotgun sequence.
ACCESSION   DASUEV010000306 DASUEV010000000
VERSION     DASUEV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308601"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..415
                     /locus_tag="VFV55_09645"
     CDS             149..415
                     /locus_tag="VFV55_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944603.1"
                     /translation="MAEPIFNARVTDLASRIYVDLVRDAAARPEGGAKSLADAERLAK
                     LSFKLAESFHRVLDELNAENEPKNVGFTVQLDDIAKWSQPQGKP"
     gene            442..1302
                     /locus_tag="VFV55_09650"
     CDS             442..1302
                     /locus_tag="VFV55_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX4944604.1"
                     /translation="MGNSHEDGFARLADWIGRTETRTDTACAAPVMALAALLDRDDAP
                     ARTGDAIPPLAHWLYFLPMHRQSEMGPDGHAKRGEFLPPVPLPRRMWAGSRLEFLRPV
                     AIGAAIRRVSRIAGVKVKEGRTGPLVFVTVRHEVSDTGGLAIADEHDIVYRGDTGRAV
                     AEAVPAAKNEAWSRVIHPDPALLFRYSAVTFNGHRIHYDHPYVTKVEGYPGLVVHGPL
                     IATLLVDLLRRSRPEACLRSFAFRAIRPLFDTAPFTVCGLPDDPAPSATLWTRDAEGA
                     VTMEATATWQ"
     gene            1337..2044
                     /locus_tag="VFV55_09655"
     CDS             1337..2044
                     /locus_tag="VFV55_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX4944605.1"
                     /translation="MAKLNLLLVPGLLCDQRLWQAQVEGLVHAAECKVADVAKNDSIA
                     AMARSAIAKMPPGPFAVAGLSMGGYVALEIHAQAAARVIGLALLDTNARADTEQATDD
                     RRRMMKMAETDFERVVNALIPKLLLPLHQRDAKLLATVKAMAASTGKEAYCRQQEAII
                     GRTDSRPRLAQVKCPTLILCGKEDVLTPVALHQEMANAIAGSRLVVAEQCGHLSTLEQ
                     PRLVTLNLVHWLSGLKA"
     gene            complement(2045..5302)
                     /locus_tag="VFV55_09660"
     CDS             complement(2045..5302)
                     /locus_tag="VFV55_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX4944606.1"
                     /translation="MSDASQIPDLVERRRALDPASSFIVQAPAGSGKTELLIQRCLVL
                     LAIVERPEEIAAITFTRKAAAEMRSRIFAALAGARTRERPVQAHEAMTWDLARAVLER
                     NDRLDWRLEENATRLRLQTIDSLCASLTRQMPVLARLGAQPDSIEDATPLYREAARAT
                     LALLEGGGRDADDVARVLAHLDNDAARVEGLLAGLLQRRDHWLRYLHVADQRGALEAA
                     LADVRREAVECAAARLADAGKAGPGGAGAVDAQAWIAFANELLTKKGEWRKGPPLAKE
                     LAGNVPVLESLRALQDLPPERYDDVQWEALGALVRVATLAVGHLKVAFAAHGQADFAE
                     IAQGALAALETPEGPTDLLLALDYRIRHILVDEFQDTSLTQYQLLAKLTAGWERGDGR
                     TLFVVGDPMQSIYRFREAEVGLFLRARHEGIGSVALAPLTLSANFRSQAGIVDWVNDA
                     FAGVMPPREDIATGAVPYTPSHAVHDREAQAVTIHPFFNGDREGEAERVAMLARAALE
                     EPVAEGSQPGTVAILVRARTALAAIVPALRSSGLRLRAIEIDPLPARPVVRDLLALTR
                     ALSHLADRTAWLALLRAPWCGLALAELHALAGVDPAGAAPGERAEDVTILERLHDGSR
                     IATLPAQAQERLRRLRAVLDAALAARGRGTLRDAVEGAWLALGGPACVGEAAALGEAD
                     AFLDHLEQEEAAAQLPDIAAFEEGLAKLYAPPDEEADPRLQVMTIHKAKGLEFDTVIV
                     PGLGGGVPPEERELFLWMERPRAGRGGGQTDLLLAPVNAAGSDRDSIHEYIRALDKRK
                     GDLENGRLLYVAATRAKRRLHLLGDAKLGDASDPAALKGPRKGSLLAKLWPVAERVFA
                     GESAAPPGSRGRHAPRPAAPAGIARVRLDFEVPAPPPGVTWRSPAEESRAAEPIEFSW
                     AGETARLAGTVVHRWLQRMAEDSLQGWDAARVRALAPQLRAELRWRGILVADLDDTTA
                     RVGRALMQAVTDPKGRWVLGPHPQAAAEYRLTALVGGRRRAFVMDRVFTDAAGERWIV
                     DYKTGTHEGAEAESFLDSERTRYAEQLHRYADALGGGPRLGLYFPLIPGWREVQA"
     gene            complement(5295..>6634)
                     /locus_tag="VFV55_09665"
     CDS             complement(5295..>6634)
                     /locus_tag="VFV55_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX4944607.1"
                     /translation="TLFQPESPDVPIQVLGILESAGLDFDHLWVMGLTDDAWPLAARP
                     NPFLPIRVQRAAGIPEADAASSFALDQRITQGWLRAAPEVVVSHSRARGDAEVAPSPL
                     IAGVAQADGELRGTEPLAAWRDAIHGAAALERLVDERGPALAGAAGAGGTALFRDQAA
                     CPFRAFAAHRLGSASPEVPQPGLSAADRGTLLHAVLAAAWEALRDKGRLDSTRDDELD
                     AILVPAADAALEKIRRRRPHALDGRFAILERQRLVSLAREWLRFERVRGDFTVASTEG
                     EVPATFGGVTVNVKLDRLDRLASGGCAIIDYKTGSASVSGWLGPRPDEPQLPLYALAS
                     GEDVAAVAIARVKVGENEFKGLARAENLLQGVTLVTKSRARLAANYADWAALREGWQR
                     ELDALGRAFAAGDARLDPKRGEKTCAGCDQALLCRVAENPPMATNTGEEEGDE"
BASE COUNT          979 a         2378 c         2323 g          954 t
ORIGIN      
        1 ggccaacgtc gaccggctcg aaggcgaagt ggcgcaattg cgcgagacgg tggcgaggct
       61 ctgccgggaa ctgggcgtat gagccgcacc gtgcctgccg agacacgggc gtaggattgt
      121 ccgtccgaac ccatcgagtc gcttcgccat ggccgaaccc atcttcaatg caagagttac
      181 cgacctcgcc tcgaggatct acgtcgatct cgttcgcgat gccgcggccc ggcccgaggg
      241 cggagcaaag tcgctggcgg atgccgaacg cctcgcgaag ctcagcttca agcttgcgga
      301 atcctttcat cgcgtcctgg acgaactgaa cgccgaaaac gagcccaaga acgtcgggtt
      361 cacggtgcag ctcgacgaca ttgccaagtg gagccaaccg cagggcaagc cgtgagagcc
      421 gcgccgccgg gggcgaccga catgggcaat tcccacgaag acggtttcgc ccggcttgcc
      481 gactggatcg gccgcacgga gacccgcacc gatacggctt gcgccgcgcc ggtcatggca
      541 ctcgccgccc tcctcgaccg cgacgacgcg cctgcgcgga ccggcgatgc cattccgccc
      601 ctggcgcatt ggctctactt tctgcccatg caccggcaat cggaaatggg tcccgacggg
      661 cacgcgaagc gcggcgaatt cctgccgccc gtgccgctcc cgaggcgcat gtgggccgga
      721 agccggctcg agttcctgcg ccctgtcgcc atcggcgccg cgatccggcg agtgtcgcgc
      781 atcgccggcg tgaaggtgaa ggaagggcga accgggccgc tcgtcttcgt gaccgtgcgc
      841 catgaggtga gcgacacggg aggactggcg attgccgacg agcacgacat cgtttaccgc
      901 ggcgacaccg ggcgagccgt ggccgaggca gtgcccgcgg cgaagaacga agcgtggtcg
      961 cgcgtgatcc atccggaccc ggcgctcctg ttccgctact cggcggtcac gttcaatggc
     1021 caccgcatcc actacgacca tccgtacgtc acgaaggtgg agggctatcc cggcctcgtg
     1081 gttcacggcc cgctcattgc gacgctgctc gtggacctgc tgcgccgcag tcgccccgag
     1141 gcctgcctca gaagcttcgc tttccgtgcg atccgcccgc tgttcgacac cgcgccgttc
     1201 acggtctgcg ggttgcccga tgatcccgcg ccttcggcga cgctttggac gcgcgacgcc
     1261 gagggcgcag tgaccatgga agcgaccgcc acctggcagt gacgcgcacc cccaacccga
     1321 ctgaaagaag atcccgatgg ccaaactgaa cctgctcctc gtgccgggcc tcctctgcga
     1381 ccagcgactc tggcaggcgc aggtcgaggg cctcgtccac gccgccgagt gcaaggtggc
     1441 ggacgtcgcg aagaacgact ccatcgcggc catggcccgg tcggccatcg cgaagatgcc
     1501 accgggacct ttcgcggtgg ccggcctgtc gatgggaggc tatgtcgcgc tggagatcca
     1561 tgcgcaggcc gccgcgcggg tgatcggcct cgccctcctc gacaccaacg cgcgcgccga
     1621 taccgaacag gcgaccgacg accgccggcg catgatgaag atggccgaga ccgatttcga
     1681 acgcgtggtg aacgccctca tccccaagct gctcctgccc ttgcatcagc gcgacgcgaa
     1741 gctgctggcc acggtgaagg cgatggccgc cagcaccggc aaggaggcgt actgcaggca
     1801 gcaggaggcc atcatcggac gcaccgacag ccggcctcgc ctcgcgcagg tcaagtgccc
     1861 gacgctcatc ctctgcggca aggaggacgt gctcacgccc gtggcgctgc accaggagat
     1921 ggccaacgcg atcgcgggtt cccgcctcgt ggtcgccgaa cagtgcggtc atctttcgac
     1981 gctcgaacaa ccccgactgg tgacgctgaa cctcgtgcac tggctgtcgg gactgaaggc
     2041 ctgatcaggc ctggacctcc cgccagcccg ggatgagcgg gaagtagagc cccagccgcg
     2101 gccccccgcc cagggcgtcc gcgtatcggt gcagctgttc cgcgtatcgc gtgcgctcgc
     2161 tgtcgaggaa cgattccgcc tccgcaccct cgtgggtgcc cgtcttgtaa tcgacgatcc
     2221 agcgctcgcc cgccgcgtcg gtgaatacgc gatccatgac gaaggcacgg cgcctgccgc
     2281 cgacgagcgc ggtgaggcgg tactcggcag cggcctgcgg gtgcggcccc agcacccagc
     2341 gccccttcgg atcggtgacg gcctgcatga gcgcgcgacc tacgcgcgcc gtcgtgtcgt
     2401 cgagatccgc cacgaggatg ccgcgccagc gcaactcggc gcgaagctgc ggcgccaacg
     2461 cgcgtacgcg cgcggcatcc cacccctgca atgaatcctc cgccatgcgt tgcagccagc
     2521 ggtggacgac ggttcccgcg agccgcgccg tttcacccgc ccacgagaat tcgatcggct
     2581 ctgcagccct ggactcctct gcgggcgagc gccaggtcac gcccggcggc ggcgcgggca
     2641 cctcgaaatc gagtctcacg cgcgcgatgc ctgccggggc agccgggcgc ggcgcgtgcc
     2701 ttccgcggga gccgggcggc gccgcgctct cgccggcgaa cacgcgttcg gcgacgggcc
     2761 agagcttcgc gaggagcgaa cccttccgcg gtcccttgag cgcggccggg tccgacgcgt
     2821 cgccgagctt cgcatcgccc aggaggtgca gccggcgctt cgcccgcgtg gcggcgacgt
     2881 agaggaggcg gccgttctcg agatcgccct tgcgcttgtc gagggcgcga atgtactcgt
     2941 ggatcgagtc cctgtcgcta cccgcagcgt tgaccggggc aagcaggagg tcggtctgcc
     3001 caccgccgcg gccggctcgc gggcgctcca tccacaggaa gagctcgcgt tcctccggcg
     3061 gcacgccgcc accgagcccc gggacgatca ccgtgtcgaa ctccagtccc ttcgccttgt
     3121 ggatcgtcat gacctgcagt cgcgggtcgg cttcctcgtc cggcggcgcg tagagcttcg
     3181 cgagcccttc ctcgaacgcc gcgatgtcgg gaagttgcgc ggcagcctct tcctgctcga
     3241 ggtgatcgag gaatgcgtcc gcctcgccga gggcggcggc ttcgcccacg catgcggggc
     3301 cgcccagcgc gagccatgca ccctcgaccg catctcgaag cgtgcctcgc ccgcgcgcgg
     3361 ccagcgccgc gtcgaggacc gcgcgaagac gtcgcagccg ctcttgcgcc tgcgcgggca
     3421 gcgtggcgat gcgggacccg tcgtgcagcc gctcgaggat cgtgacgtcc tccgcgcgtt
     3481 cgcccggtgc cgcgccggcc ggatccacgc cggccagcgc gtgcagttcc gcgagcgcga
     3541 gcccgcacca cggcgcgcgc agcagggcaa gccacgccgt gcgatcggcg aggtgcgaga
     3601 gcgcccgcgt gagtgcgagc aggtcgcgca cgacggggcg cgcgggcaag gggtcgatct
     3661 cgatcgcgcg cagcctcagc cctgacgacc ggagcgccgg cacgatggca gcgagcgccg
     3721 tgcgggcgcg cacgaggatc gcgacggttc cgggctggct gccttccgca acgggctctt
     3781 cgagggcggc cctggcgagc atggccaccc gctcggcctc gccctcgcga tcgccgttga
     3841 agaagggatg gatcgtcacg gcctgcgcct cgcgatcgtg aaccgcgtgc gatggcgtgt
     3901 acggcacggc gccagtggcg atgtcctcgc gtggcggcat cacacccgcg aaggcatcgt
     3961 tcacccagtc cacgatgcct gcctgcgatc ggaagttggc cgagagcgtg agcggggcga
     4021 gcgccacgct gccgatgccc tcgtggcgcg cgcgcaggaa caggcccacc tccgcctcgc
     4081 ggaagcggta gatcgactgc atcgggtcgc ccacgacgaa aagcgtgcgg ccgtctccgc
     4141 gctcccaccc tgcggtgagc ttcgcgagca gctggtactg ggtgagggag gtgtcctgga
     4201 actcgtcgac caggatgtgg cggatgcggt agtccagggc caggaggagg tcggtgggcc
     4261 cttccggcgt ctcgagcgct gccagcgcgc cctgtgcgat ctcggcgaaa tccgcctgcc
     4321 cgtgcgcggc gaacgcgacc ttgagatgac ccaccgccag cgttgcgacg cgcaccaggg
     4381 caccgagcgc ttcccactgg acgtcgtcat agcgctccgg cggcaggtcc tgcagcgcgc
     4441 gcagggactc gagaaccggc acgttgcccg cgagttcctt cgcgagcggc ggccccttgc
     4501 gccattcgcc cttcttcgtg aggagttcgt tggcgaaggc gatccaggct tgcgcgtcca
     4561 cggcgccagc gcctcccggc cccgcctttc ccgcgtcggc gagccgggcc gcggcgcatt
     4621 cgacggcctc gcgacgcacg tccgcgagcg cggcttcgag ggcgccacgc tggtccgcca
     4681 cgtgcaggta gcgcagccag tggtcgcgac gctgcagcag cccggcgagc agcccttcca
     4741 cccgggccgc gtcgttgtcg aggtgcgcga ggacgcgcgc cacatcgtcg gcgtcgcggc
     4801 cgcccccctc gagaagcgcc agcgtggccc gcgcggcctc gcgatagagg ggcgtggcgt
     4861 cctcgatgct gtcgggctgc gcgccaaggc gcgcgagcac cggcatctgg cgcgtaagcg
     4921 acgcgcacaa cgaatcgatc gtttgcaggc gcaggcgcgt ggcgttctcc tccagccgcc
     4981 agtcgagccg gtcgttgcgc tcgaggaccg cgcgcgcgag gtcccaggtc atcgcttcat
     5041 gcgcctgcac ggggcgctcg cgcgtgcgcg ctccggccag cgccgcgaag atgcggctgc
     5101 gcatctcggc ggcagccttg cgcgtgaagg tgatggcggc gatttcctcc ggccgctcga
     5161 cgatcgccag caggaccagg cagcgctgga tcagcagctc ggtcttgccg gagccggccg
     5221 gcgcctggac gatgaaggac gatgcgggat cgagcgcgcg acgccgctcg acgaggtccg
     5281 ggatctggct tgcgtcactc atcgccctct tcctcgcctg tgttcgtggc catgggcggg
     5341 ttctccgcca cgcggcaaag gagcgcctgg tcgcaccccg cgcaggtttt ttcgccgcgt
     5401 ttgggatcga gccgcgcatc gcccgcggcg aaggcgcggc cgagtgcatc gagttcgcgc
     5461 tgccagcctt cgcgcagcgc tgcccagtcc gcgtagttcg cggcgaggcg ggcgcggctc
     5521 ttcgtgacga gggtgacgcc ctgcaggagg ttctccgccc gcgcgagccc cttgaattcg
     5581 ttttcgccga ctttcacgcg ggcgatcgcg acggcagcca cgtcttcgcc gctcgccagc
     5641 gcgtacaggg gcagttgcgg ctcgtccggt cgcggcccga gccagccgga caccgacgcg
     5701 cttcccgtct tgtagtcgat gatggcgcag ccaccgctgg caaggcggtc gagccggtcg
     5761 agcttcacgt tcacggtgac gccgccgaag gtcgccggaa cctcgccttc ggtcgatgcg
     5821 accgtgaaat cgccgcggac gcgctcgaaa cgcagccact cgcgcgcaag agataccagt
     5881 cgctggcgct cgaggatggc gaagcggcca tcgagcgcat gcgggcggcg gcggcggatc
     5941 ttctccaggg cggcatccgc ggccggaacg aggatggcgt cgagctcgtc gtcgcgggtg
     6001 gagtcgaggc gacccttgtc ccggagcgcc tcccacgccg ccgcgagcac ggcatgcagg
     6061 agcgtgccgc gatcggccgc ggaaagcccc ggctggggca cctcgggcga agccgagccg
     6121 agtcggtgtg cagcgaaagc gcggaacggg cacgcggcct ggtcccggaa gagcgccgtg
     6181 cctcccgcgc ccgccgcacc tgcgagtgcc ggaccgcgct cgtcgacgag ccgttccagt
     6241 gcagcggcac catggatcgc gtcgcgccac gcggctagcg gttcggttcc ccgcagctcg
     6301 ccgtcagcct gcgcgacacc ggcgatgagc gggcttggcg caacctcggc gtcgcccctg
     6361 gctcgcgaat ggctgacgac aacctccggc gccgcgcgca gccagccctg cgtgatccgc
     6421 tgatccagcg cgaacgacga agcggcgtcg gcctcgggaa tgccggccgc gcgctgcacc
     6481 cggatgggca ggaagggatt gggccgcgcg gcaaggggcc acgcgtcatc ggtgagcccc
     6541 atcacccaca ggtggtcgaa atcgagaccc gcgctttcga ggatgcccag cacctggatc
     6601 ggaacgtcgg gcgattcggg ctggaacagc gtgc
//
LOCUS       DASUEV010000307         5208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_653719, whole genome shotgun sequence.
ACCESSION   DASUEV010000307 DASUEV010000000
VERSION     DASUEV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653719"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..783)
                     /locus_tag="VFV55_09670"
     CDS             complement(115..783)
                     /locus_tag="VFV55_09670"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX4944608.1"
                     /translation="MSAVRRRNPRAEARAQSQRERILDAAQKCFIEHGFHGASMATIA
                     ETADMSPGLMYRYFESRSAIVLAIIGRELEGKRAKIGELYTGADIVTRVLETFRQWQA
                     GDPEAMNAALFLEMTAQATREPEIARAIRVSDKLTRREFERWMCRPREEGGLGFPKDV
                     AQSRAILMQCLIEGLAVRALRDPDLDARTLKGALEPLFLLLGLAQDDPPARRVRAGTS
                     GPSR"
     gene            complement(780..2189)
                     /locus_tag="VFV55_09675"
     CDS             complement(780..2189)
                     /locus_tag="VFV55_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX4944609.1"
                     /translation="MNRITRMPIALAAALLASACATLEPALPEAIPSIPAQWPSSSTV
                     ATAPATPITGIADVGWRDFFVDPRLAQLIARSLENNRDLRVAVLNVERARGLYRIQRA
                     ARVPSVGVAGTLTRSGGDHRDVNDAYAVSLGITEFELDLFGRVRNLSQAALEQYFAQE
                     EARRSAQLSLVAEVANAYLTLAADQELMRVAQATREIEDAAYRLTQKRHEIGAVSGLE
                     LNQARLSVETARADAARFAGQVAKDANALALLVGAPVEPDLLPEDFDRHLSGLQALPA
                     GLPSEVLLRRPDVLQAEHALRAANANIGAARAAFFPSIRLTGAVGTASDELSGLFRSG
                     SFAWSVLPRIDVPIFQGGRLVANLDVARADRDIALALYEKSIQAGFREAADALSLVGT
                     LAEQRLAQESLLDAASQADRLSRSRYEAGRDSYLVLLDAQRTLYAARQGLVATRLAEQ
                     ANRVTLYKVLGGGWKERNP"
     gene            complement(2182..>5208)
                     /locus_tag="VFV55_09680"
     CDS             complement(2182..>5208)
                     /locus_tag="VFV55_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4944610.1"
                     /translation="VTVSATYPGASAKAVEDSVTQVIEQSMTGLDGLLYISATSEANG
                     TATITLTFDSSVNPDTAQVQVQNKLALATVSLPQIVQQQGIRVSKANPGFLMVMGFVS
                     QDGSMEQRDIADYVKATIADPISRVDGVGSVQVFGARYAMRVWLDPGKLDTYRLSPAD
                     VIAAVRAQNAQVAVGQLGGTPAIPGQQLNATITGQDRLQTPEQFRAIVVRSRADGSAL
                     TLGDVARVELGAEDYSIIARYNGQPSTGVAISLATGANSLATAQRLEALIKQLEPAFP
                     PGLKAVIPFDTTPFVTASIKEVVKTLLEAVALVFLVMYLFLQNFRATLIPTIAVPVVL
                     LGTFAVLAVLGYSINMLTMFAMVLAIGLLVDDAIVVVENVERVMSEQGLTPLEATRQS
                     MDQITGALVAVGMILAAVFVPMAFLGGSTGVIYRQFSATIVSAISLSVLVAIVLTPAL
                     CATMMVRIEKGHHASDTGFFGWFNNVFDRGNRSYQKTVRGITGRSKRFFAVFLAMAAV
                     MVVLFLRLPTAFLPAEDQGVLFAQVQGPVGATQERTMEVMRKIERHFLDNEKDAIKSL
                     FTVQGFSFGGFGQNTGVAFVSLKDWSERTTPALSVEAVAGRAMGKFMQIQDAFVFAFA
                     PPAVPELGVAGGYAFYLKDNVGMGHDALTQARNQFLGMAMQSKLLANVRPNGQEDTPQ
                     FRIDIDTAKAGALGLLDADINAALSVAWGSQYIDDFIDRGRVKRVIVQADAPFRMVPE
                     DFRRWTVKNAKGDMVPFTAFATSHWEYGSPRLERYNGVPAMQINGEAARGVSSGDAMA
                     EVERLVGQLPKGVGLEWTGASYQERAAGAQTPMLYTLSILVVFLCLAALYESWSVPTA
                     VLMVAPLGILGTVLATMLRGYERDVYFQVAMLATVGLSSKNAVLIIQFAKENLEKGMD
                     VIEAVVAAGRDRLRPILMTSIAFGLGVMPLASATGAGSGAQRAIGTGVLGGMVVGTFL
                     GIAFIPLFFVVVQRAFVRKKTAAPTLEPAAGGQGHE"
BASE COUNT          816 a         1800 c         1775 g          817 t
ORIGIN      
        1 ggatgagctc cgaggaaatg tccatcagga ggctcacgcc gccgagcgcc cagatcgccg
       61 gcgggatacg gaccttcccg gcggccacga cgccggggag ccgtttttcg gtcgtcatcg
      121 cgacgggccg gacgtccctg ccctgactct ccgcgccggc ggatcgtcct gcgcaagccc
      181 caggagaaga aacagcggtt cgagcgcacc cttgagcgtt cgcgcgtcga ggtcaggatc
      241 gcgcagggcc cgcaccgcaa gcccttcgat caggcactgc atcaggatcg cgcgggactg
      301 ggcgacatcc ttcgggaatc ccagcccgcc ctcctccctg ggccggcaca tccagcgttc
      361 gaattccctg cgcgtgagct tgtccgacac gcggatcgcc cgcgcgatct cgggttcccg
      421 cgtggcctgc gcggtcatct ccagaaacag cgccgcgttc atcgcctcgg ggtcgcccgc
      481 ctgccattgg cggaacgttt cgaggaccct ggtgactatg tccgcgcccg tgtacagctc
      541 cccgatcttg gcgcgcttgc cttccagctc gcgcccgatg atcgccagga cgatggcact
      601 gcggctctcg aagtagcggt acatcaggcc ggggctcatg tccgcggtct cggcgatggt
      661 cgccatgctg gcgccgtgaa agccgtgctc gatgaagcat ttctgcgccg cgtcgagaat
      721 gcgctcgcgc tgcgactgcg cgcgcgcctc ggcccgcggg ttcctgcgcc gcaccgcgct
      781 catggattgc gctctttcca gccgcccccg aggaccttgt acaaggtgac acggttggcc
      841 tgttcggcca ggcgcgtggc gacgaggccc tgcctggcgg catagagcgt gcgttgcgcg
      901 tcgagaagca ccaggtagct gtcgcgcccg gcctcgtagc gcgagcgcga aagccggtcg
      961 gcctgggatg ccgcgtccag cagcgattcc tgcgccaggc gctgctcggc gagcgttccg
     1021 accagcgaca acgcatcggc ggcctcgcgg aacccggcct ggatcgactt ctcgtagagc
     1081 gccagcgcga tgtcgcgatc cgccctggcg acgtcgaggt tcgcgaccag tcgcccgccc
     1141 tggaagatcg gcacgtcgat gcgcggcagg acgctccagg cgaaggaacc gctccggaaa
     1201 agccccgaca attcgtcgct cgccgtgccg acggcaccgg tgagccggat cgacgggaag
     1261 aacgccgcgc gggcggcgcc gatgttcgcg ttggccgcgc gcagcgcatg ctcggcctgc
     1321 aatacgtcgg ggcggcgcag cagcacttcc gacggcagcc cggcgggaag cgcttgtagc
     1381 ccggagagat gcctgtcgaa atcctccggc aacaggtccg gttccaccgg ggcgccgacc
     1441 agcaacgcaa gcgcattggc atccttcgcc acctgcccgg cgaatcgcgc cgcgtccgcg
     1501 cgcgccgtct cgaccgacaa gcgggcctgg ttgagctcga gaccggatac ggcaccgatc
     1561 tcgtggcgct tctgcgtgag gcgataggcg gcgtcctcga tctcgcgggt ggcctgtgcc
     1621 acgcgcatca gctcctggtc cgccgcgagg gtcaggtagg cattggccac ttccgcgacg
     1681 agactgagct gggcgctgcg ccgtgcttcc tcctgggcga aatactgctc cagcgccgcc
     1741 tggctcaggt tgcgtacgcg gccgaaaaga tccagctcga actcggtgat gcccaggctc
     1801 actgcgtagg catcgttcac gtcgcggtgg tcgccgcccg atcgcgtgag cgttcccgcg
     1861 acgcccaccg agggcacgcg ggcggcgcgc tggatgcggt acaggccgcg cgctctctcg
     1921 acattgagca cggccacgcg caggtcgcgg ttgttctcga gggaccgcgc gatgagctgc
     1981 gcgagcctgg gatcgacgaa gaagtcgcgc cagcccacgt ccgcgatgcc ggtgatgggc
     2041 gtggccggcg ccgtagcaac ggtcgaagac gacggccact gcgccgggat ggacggtatt
     2101 gcctcgggca acgcgggctc cagcgtcgcg caggcggacg cgagcaacgc cgccgccagc
     2161 gcgatcggca tgcgcgtgat tctattcatg gccctgtcct cctgcggccg gctccagcgt
     2221 gggcgcggcg gtcttcttcc gcacgaaggc gcgctgcacg accacgaaga agagcgggat
     2281 gaaggcgatg cccaggaagg tgccgacgac catgccgccc agcactcccg tgccgatggc
     2341 gcgctgcgcc ccggagccgg caccggtggc gctggcgagc ggcatcacgc ccagtccgaa
     2401 ggcgatcgag gtcatgagga tcggccgcag gcggtcgcgc ccggcggcca ccacggcctc
     2461 gatcacgtcc atgcccttct cgaggttctc cttggcgaac tggatgatga gcaccgcgtt
     2521 cttgctcgac agccccaccg tcgccagcat cgcgacctgg aagtacacgt cgcgctcgta
     2581 gccgcgcagc atcgtggcga gcaccgtgcc gaggatgccc agcggcgcca ccatcaggac
     2641 cgccgtgggc acgctccagc tctcgtagag cgccgcgagg cacaggaaca cgacgaggat
     2701 cgaaagggtg tagagcatcg gcgtttgcgc gccggccgcg cgctcctggt aggaggcgcc
     2761 cgtccactcc aggcccacgc ccttcggtag ctgcccgacg aggcgctcga cttcggccat
     2821 ggcgtcgccc gaactcacgc cgcgcgccgc ctcgccgttg atctgcatgg cgggcacgcc
     2881 gttgtagcgc tcaaggcgcg gcgagccgta ttcccagtgc gaagtggcga aggcggtgaa
     2941 cggcaccatg tcgcctttcg cgttcttcac cgtccagcgg cggaagtcct ccggcaccat
     3001 gcggaatggc gcgtcggcct gcacgatcac gcgcttcacg cgcccgcggt cgatgaagtc
     3061 gtcgatgtac tggctgcccc aggccacgga aagcgcggcg ttgatgtccg cgtcgaggag
     3121 ccccagcgcg cccgccttgg cggtgtcgat gtcgatgcgg aactgcggcg tgtcctcctg
     3181 gccgttggga cgcacattgg cgagcagctt gctctgcatg gccatgccga ggaactggtt
     3241 cctcgcctgc gtgagcgcgt cgtggcccat gccgacgttg tccttgaggt agaaggcata
     3301 gccgccggcc acgcccagtt ccggcaccgc gggcggcgcg aaggcgaaca cgaacgcgtc
     3361 ctggatctgc atgaactttc ccatggcgcg cccggcgacc gcctcgacgg aaagcgccgg
     3421 cgtcgtgcgt tccgaccagt ccttgaggct cacgaaggcc acgcccgtgt tctggccgaa
     3481 gccgccgaag ctgaatccct gcaccgtgaa gagcgacttg atcgcgtcct tctcgttgtc
     3541 gaggaaatgc cgctcgatct tcctcatcac ttccatcgtg cgctcctggg tcgcgcccac
     3601 cgggccctgc acctgggcga acagcacgcc ctggtcctcg gccggcagga acgcggtggg
     3661 caggcgcagg aagagcacca ccatcaccgc cgccatggcg agaaacaccg cgaagaaccg
     3721 cttgctgcgc cccgtgatgc cgcgaaccgt cttctggtag ctgcgattgc cgcggtcgaa
     3781 gacgttgttg aaccagccga agaagcccgt atccgaggcg tggtggccct tttcgatccg
     3841 caccatcatc gtcgcgcaca gcgccggcgt gaggacgatc gccaccagca cggagagcga
     3901 gatggccgag acgatcgtcg ccgagaactg ccggtagatg acgccggtgg atcctcccag
     3961 gaacgccatc ggcacgaaca ccgctgcgag aatcatgccc acggcgacca gcgcgccggt
     4021 gatctggtcc atcgactggc gcgtcgcctc cagcggcgtg aggccctgct cgctcatcac
     4081 tcgctcgacg ttctccacca cgacgatggc gtcgtcaacc agcaggccga tggcgagcac
     4141 catggcgaac atagtgagca tgttgatcga gtagcccagc acggcgagca ccgcgaacgt
     4201 gcccagcagc accaccggca ccgcgatcgt ggggatgagc gtggcccgga agttctgcag
     4261 gaagaggtac atcaccagga acacgagggc gaccgcctcc aggagggtct tcaccacctc
     4321 cttgatcgag gcggtcacga acggggtcgt gtcgaagggg atgaccgcct tgagccccgg
     4381 cgggaaggcg ggttccagct gcttgatcag cgcctccagg cgctgcgccg tggcgagcga
     4441 gttggcgccc gtggcgagcg agatcgccac gccggtggac ggctgcccgt tgtagcgcgc
     4501 gatgatgctg tagtcctcgg cgcccagttc cacgcgcgcc acgtcgccga gcgtgagcgc
     4561 cgagccgtcg gcacggctgc gcacgacgat cgcgcggaat tgctcgggcg tctgcaggcg
     4621 atcctgcccg gtgatcgtcg cgttgagttg ctggccgggg atcgcgggcg tgccgccgag
     4681 ctggcccacg gcgacctgcg cgttctgcgc gcggaccgcg gcaatgacgt cggccggcga
     4741 aagccggtag gtatccagct ttcccggatc gagccagacg cgcatcgcgt agcgcgcgcc
     4801 gaagacctgc acgcttccca cgccgtcgac gcggctgatc gggtccgcga tggtcgcctt
     4861 cacgtagtcg gcgatgtcgc gctgttccat gctgccgtcc tgcgagacga aacccatgac
     4921 catgaggaag ccggggttcg ccttgctcac gcggatgccc tgctgctgca cgatctgcgg
     4981 cagcgacacg gtggcgagcg cgagcttgtt ctgcacctgc acctgcgccg tatcggggtt
     5041 gacggagctg tcgaaggtga gcgtgatcgt cgcggtgccg ttggcctcgc tggtcgcgga
     5101 aatgtagagg agcccgtcca ggcccgtcat gctctgctcg atcacctgcg tgaccgagtc
     5161 ctcgacggcc ttggccgagg cgcccgggta ggtggcgctg accgtgac
//
LOCUS       DASUEV010000308        14435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1680679, whole genome shotgun sequence.
ACCESSION   DASUEV010000308 DASUEV010000000
VERSION     DASUEV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680679"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..374
                     /locus_tag="VFV55_09685"
     CDS             165..374
                     /locus_tag="VFV55_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEX4944611.1"
                     /translation="MAYESEATRFLRDLLDNRPELREQRRRNRATWWDKPQDLEVQQE
                     REAAKVAQGAYVYFPPPRPPEAPGS"
     gene            complement(371..1219)
                     /gene="serB"
                     /locus_tag="VFV55_09690"
     CDS             complement(371..1219)
                     /gene="serB"
                     /locus_tag="VFV55_09690"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX4944612.1"
                     /translation="MTGFNLVIQGAEVATSDLKSLCVLARGRTIERITGEAFRIRDAD
                     PSMRDAVAARCAAAALDWGFVEDGRRLADFGLLALDMDSTLITVECIDEIADFAGRKA
                     EVAAVTAAAMRGEIDWSQSLLSRVAALAGLDAAVLERVYRERLHLTPGAEELLGTAHR
                     AGLKTLLVSGGFTYFTDRLRERLGFTGAHANELVVEDGRLAGRVTGPLVDAAGKAGHV
                     ARLRESLSLPKKRVLAIGDGANDLAMMAEAGTSIAFHAKAVVRDRATYTLAQVGLEGV
                     LGLFPG"
     gene            complement(1216..4617)
                     /gene="mfd"
                     /locus_tag="VFV55_09695"
     CDS             complement(1216..4617)
                     /gene="mfd"
                     /locus_tag="VFV55_09695"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX4944613.1"
                     /translation="MARSAFARSGSSDAFWIARLARSAKPVFVVTESAQDAERLRDEV
                     AWFDPALAVHRLPDWETLPYDQFSPHPDLVSERLATLHQFSKGACDVGLIPVTTALQR
                     LAPRSYLAARTFFLRLKERLDLEALKAQLALAGYAAVQQVMAPGEFCIRGGLVDLFPT
                     GSPVPYRVDLFGEEIESIRTFDVDTQRSIYPVSEVRLLPAREFPMDEEGRARFRANFR
                     ERFEGDPTKRRAYKDVSNGIAPAGVECYLPLFFESTATFFDYLPPGATCVLHEDVPAG
                     AEAFWRDLKGRWELLRGDGDRPLLPPEQLYLPVEEFFVGLQGLPRLDIRRDATGPAPA
                     NAPVFVAANLPDVGVDRRSAEPLRALDRFVSSFDGRVLVVADGPGRRETLAQLFGEHG
                     LHPALVDDWTRFTQSPAPFMLTHGPLAAGFAAPGERFAIITEAELYPSQVRQSRRRDA
                     RGITSAEGMLRDLAEVKQGDPVVHSQHGIGRYQGLVTMDLGEGPTEFLHLEYEGGDKL
                     YVPVAQLHVISRYTGASPEDAPVHRLGSGQWEKARRKAAQQVRDTAAELLDLYARRAA
                     RQGYAFPLKAQDYEAFSGAFEFEETPDQAGAIQSVLADMTSGKPMDRLVCGDVGFGKT
                     EVAMRAAYVAVAAGKQVAILVPTTLLAEQHFENFSDRFAQWPVKLAELSRFRSPKETA
                     AAIAGLADGSIDIAIGTHKLIQKDVIFKNLGLVIIDEEHRFGVRQKEQLKRLRAEVDV
                     LTLTATPIPRTLAMSLEGLRDFSVIATAPQRRLAIRTFVARQSPGIVREAVLRELKRG
                     GQAYYLWNEVDTIENRREALEKLLPEARIAVAHGQMRERDLERVMRDFYHQRANVLLC
                     STIIETGIDVPTANTILIDRADRFGLAQLHQLRGRVGRSHHQAYAYLLTPPEDALNAN
                     AKKRLEAIQMMEDLGAGFYLAMHDLEIRGAGEVLGESQSGGIHDVGFALYTEMLEHAV
                     RSLRAGKEPDLSRLVTVATEVNLHAPALLPEAYCGDVHERLVIYKRLANCASDEDLQR
                     LTEELVDRFGELPEPARVLLECHRLRIHGAPLGVSRIDASPSAIVVHFVPDPPVDGRR
                     IIALVQSGGRYRMPGPDRVRIESAIPDLKARAGEVRRFLKGLAAP"
     gene            complement(4623..6137)
                     /locus_tag="VFV55_09700"
     CDS             complement(4623..6137)
                     /locus_tag="VFV55_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX4944614.1"
                     /translation="MTTIRQDDLIQSVADAFQYVSFYHPVDYIRALARAYELEQSPAA
                     RDAMAQILINSRMSAEGHRPMCQDTGIAIAFIRVGMDVRWDAKLTLQQMVDEGVRRAY
                     TDKDNPLRASVLSDPDGKRKSTGDNTPAVVHVELVPGDKVDVIVAAKGGGSENKSKFA
                     MLLPSDSIVDWVLSIVPTMGADWCPPGILSLGIGGTPEKAMLMAKWGMMDPINIQDLV
                     ERGPRTRAEELRLELYEKVNALGIGAQGLGGLTTVLDVKVNDYPTHAASKPVALLPNC
                     AATRHQHFVLDGSGPATFDPPDLSEWPKLALDFAKSRRVNLDTLTRADLATWKSGETL
                     LLSGKLLTGRDAAHKRMVDMMARGEKLPVDFTNRFIYYVGPVNAVREEAVGPAGPTTS
                     SRMDKFVEPMLANTGLIGMVGKSERGAIAIESIKKHGAAYCIAVGGAAYLVSKAIKKA
                     RVLAFDDLGMEAIHEFEVKDMPVTVAVDAGGNAVHRTGPAEWRAKIGKIPVVVA"
     gene            complement(6276..6911)
                     /locus_tag="VFV55_09705"
     CDS             complement(6276..6911)
                     /locus_tag="VFV55_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX4944615.1"
                     /translation="MSTPILPRPAALRYLGGFIFWTRWLQAPLYLGLIVAQAVYVVHF
                     WVELVHLVEAAFGNKAALAALIESVGYSLPEIRDASGAVVGHAAPKFNETVIMLVVLA
                     LIDVVMISNLLIMVIVGGYETFVSRLDLEDHPDQPEWLSHVNASVLKVKLATAIIGIS
                     SIHLLKTFINADNYSEKVLIAQTGIHIAFLFSAMAIAACDRIMPSPVANEH"
     gene            complement(6921..8588)
                     /locus_tag="VFV55_09710"
     CDS             complement(6921..8588)
                     /locus_tag="VFV55_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX4944616.1"
                     /translation="MNARAAPPFAPFPPWKWEVPARLNIGVACTDAHLGTPAADRVAM
                     IVEDDALGTRAITYRALAERTSRFAELLRHLCIGAGERVLIRLPNCIDYPTAFLGAMK
                     RGAVSVPTSTLLTAEEVEYLAHDSGAQAIVIDKAAWKAMGTRLAGKESLRFALLSGAG
                     EVTPCKGVEVIDLEAALAGIATWEPAEDTAVDDPAYLVYTSGTTGYPKGVLHGHRSLI
                     GRTPAASYWFDFRGPEGSGATDRTDRIVHSGKFNWTYVLGSALMDPLYHGKTVIAHEG
                     KNDAETWPRLIAKHGATIFIGVPTIYRQILQKTAFTRADVPTLRHCMSAGEHLSDEVF
                     GLWKDRFGLDIFEAVGMSEFSYYLSQSKRRPIRPGSAGFPQPGHEIRLLDPETLREVA
                     AGEEGMICVPESDPGLFLRYWNLPEETARLRHGGWFLTGDYARYDAEGYLWFLGRKDD
                     IIKSFGYRVSPYEVERVLKSHPAVADCACVGEEAGKDKLLVVAYVIEHAGKSVTPDDL
                     AAFGREHLAAYKAPKVVYLAREFPRTRNGKILRREITPAIALARSSG"
     gene            complement(8585..9721)
                     /locus_tag="VFV55_09715"
     CDS             complement(8585..9721)
                     /locus_tag="VFV55_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4944617.1"
                     /translation="MNVPQHAPSPASAAAAPEILGDYWPGIQIYYPPVKFAPSLGIYE
                     DLEQAAQRFKKHAWNTTSHTLLFDLEDGCRQKEMSRELLRRELPGLPRKREVQIAVRI
                     NPFRTEEYEKDLALVRDLCDHFDVVMLAKAGEAYGAPEIRDLSAVLVGLNHGITIQPI
                     IEHPKSLKIAPELMQYATVKHVVFGIHDFSKAMGIHITPRHWTEELKFYLHDILFEAR
                     IAGKGVIGGVETLIGQSTMPENFVEPDDVRRWLDLHGDEESRVVYRHACEEASLGLSG
                     KQVIHPSHIHPCKVAFTPSPSEIRTKIAILRAAVEADALLGGAIKFQGEMLDPPMFGK
                     ALQTLLRAHALHALSPSDTDFAMDVLKRLPAQVVRENWPYGVIL"
     gene            10002..11972
                     /gene="acs"
                     /locus_tag="VFV55_09720"
     CDS             10002..11972
                     /gene="acs"
                     /locus_tag="VFV55_09720"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX4944618.1"
                     /translation="MSKIESVHQETRVFPPSEAFVKQANVSGMAAYKALCDEAERDFE
                     GFWARLARENLLWSTPFTKTLDESNAPFFKWFHDGELNASYNCLDRHLKTQPDKVAII
                     FEADDGKVTKVTYKELYHRVCQFANALKSHGIRKGERVLIYMPMSIEAVVAMQACARI
                     GATHSVVFGGFSAKSLHERIIDAGAVAVICADGQFRAGKEIALKPAVDEALAMGGCET
                     IREVIVYKRSGSPVEMQAPRDKWWHDVVQGQAQDCEPTWVNAEHPLFILYTSGSTGKP
                     KGVQHSTGGYLLLAALTMKWVFDNKPTDVFWCTADVGWVTGHTYIAYGPLAVGATEVV
                     FEGVPTYPDAGRFWKMIQDHKVNVFYTAPTAIRSLIKAGHDLPTKYDLSSLRILGTVG
                     EPINPEAWMWYYQTVGGSRCPVVDTFWQTETGAHMITPLPGATPLVPGSCTLPLPGIM
                     AAIVDETGQELPNGHGGILVVRRPWPSMIRTIWGDPERYRKSYYPEELGGKLYLAGDG
                     SIRDKETGYFTIMGRIDDVLNVSGHRMGTMEIESALVANTRLVAEAAVVGRPDDMTGE
                     AVVAFVVLKGHRPTGDAARELAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMR
                     RLLRLLAKGEEITQDVSTLENPAILEQLKHPL"
     gene            12078..13976
                     /locus_tag="VFV55_09725"
     CDS             12078..13976
                     /locus_tag="VFV55_09725"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX4944619.1"
                     /translation="MNERQPPPLLTAATADVLRKHAPFDAMREADLAHLAAGLKLRYF
                     AKDTVILSPASGRVTSLFIIQRGVVQADEPSQVSLNTMAATLTDGEVFPVGALIANRP
                     TTLRYKALRDTFCYLLDEVRFKEVMARSHEFHAFCTRRLAALLATSTRGMREAYSHRA
                     AAELTMASPLRNALRHEPVTLPETASVRQVLELMKSRRIGSIVLTGSAGGPSGIFTQT
                     DVLNRVALAGQPLDRPVSEVMTRGPAALAAGSALSEAAQLMASRGFRHVLVIDGDRLL
                     GVISERDLFGLQRLSLQGLRKDIGGATNVDALAFAAREVRELGTAMLAQGVAAEQLTQ
                     LVSALNDAITGRAIELALRDHDVADTGYCWMGLGSEGRNEQTLATDQDNAIIFPDREG
                     HDRAATRARLLAFAEQVNRTLDACGFPLCKGNIMARNPSWCLSLAEWFDRFDDWIRNS
                     DPEALLNASIFFDFRALHGDATLVDRLREFLLANVKERPVFLRQMAANAVLTRPPLGL
                     FGDIVGGEGGTVDLKRQASRVVVDCARILALAAGVGAVSTAERLRATGPKVRMSDDDV
                     ATAIDAFQFIQMLRLRAQDSLDRGTGSSEPNRIDPDSLNSLDRRILREALRQARKLQN
                     RVALDYQL"
     gene            13988..>14435
                     /locus_tag="VFV55_09730"
     CDS             13988..>14435
                     /locus_tag="VFV55_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX4944620.1"
                     /translation="MLSSLWPFRRARAGLPDEVVRRIEHWRSLADFERGRPPSSGRWV
                     VVDVESSGLDAVSDSLIAVGALAVVDGGIDLADSFEVILRQDAPSTVDNIEVHGIGGM
                     EQTEGEDPGEALAAFLAFIGKDPLVAFHAPFDSTMLRRAIDRHLGVA"
BASE COUNT         2274 a         4838 c         5029 g         2294 t
ORIGIN      
        1 gacaggaagg agcggggata ggagatccgc atcgcgcagg acccggattg ggggtgggaa
       61 ttatcctccg cgcgtgaggc gtggcgcaaa cgggcaagta atgcgcggtg ctatgattcc
      121 cgcctcgatt ccccgcccgg cttcccgccg cccgactgca agacatggcc tacgaatccg
      181 aagccacccg gttcctgcgc gacctcctcg ataatcgccc ggagctgcgc gagcagcgcc
      241 gccgcaatcg cgccacgtgg tgggacaaac cacaggacct ggaggtgcag caggagcgcg
      301 aggccgcgaa ggtggcgcag ggagcctacg tgtacttccc gccgccgcgg cccccggagg
      361 cgcccggctc ctagccggga aaaaggccga gcacgccctc cagcccgact tgggctaggg
      421 tgtacgtggc ccggtcgcgt acgaccgcct tcgcgtgaaa ggcgatgctc gtgcccgcct
      481 ccgccatcat cgccaggtca ttggcgccgt cgccgatcgc gaggacgcgc ttcttcggaa
      541 gcgacagcga ctcgcgcagg cgcgccacgt ggccggcctt tcccgcggcg tccacgagcg
      601 ggccggtgac gcggccggcc agtcgtccgt cctcgacgac gagctcgttg gcatgcgccc
      661 cggtgaaccc cagccgctcg cgaaggcggt cggtgaaata cgtgaatccc cccgacacga
      721 gcaaggtctt caggcccgcg cggtgcgcgg ttccgaggag ttcctccgcg cccggggtga
      781 ggtgcagccg ctcccggtag acgcgctcga ggaccgccgc gtccagcccg gcgagcgccg
      841 ccacgcggct cagcaggctt tgcgaccagt cgatctctcc gcgcatcgcg gcagcggtca
      901 ccgcggcgac ctcggccttg cgcccggcga aatcggcgat ctcgtcgatg cattccacgg
      961 tgatcagcgt ggagtccatg tccagcgcca ggaggccgaa gtcggcgagc ctgcggccgt
     1021 cctcgacgaa gccccaatcg agggcggctg cggcgcagcg cgcggcgacc gcatcgcgca
     1081 tcgacggatc ggcgtcacgg atccgaaaag cctctcccgt gatgcgctcg atcgtgcggc
     1141 cgcgggccag gacgcacagg gacttcaggt ccgaggtggc aacttccgcg ccctggatca
     1201 cgagattgaa gccggtcatg gcgcggccaa tcccttgagg aaccgccgca cttctcccgc
     1261 acgcgccttg aggtccggga tcgcgctctc gatccgcacg cggtcggggc cgggcatgcg
     1321 gtagcgccct cccgactgca ccagcgcgat gatgcgccgg ccgtccacgg gcggatcggg
     1381 cacgaaatgc accacgatcg ccgaggggct ggcgtcgatg cgggagacgc cgagcggcgc
     1441 gccatggatc cgcagtcgat ggcattcgag gagcacgcgg gcgggctccg gaagctcgcc
     1501 gaagcgatcg accagctcct cggtgaggcg ctgcaggtcc tcgtcggagg cgcaattggc
     1561 cagccgcttg tagatcacga ggcgctcgtg cacgtcgccg cagtaggcct cgggcagaag
     1621 cgccggcgcg tggaggttca cctccgttgc gacagtcacg agccgcgaga ggtccggttc
     1681 cttgccggcc cgcagcgacc tgaccgcgtg ctcgagcatc tccgtgtaca gggcgaagcc
     1741 cacgtcgtgg atgccgcccg attgcgactc gccgagcact tcgccggcgc cgcggatctc
     1801 gaggtcgtgc atcgcgaggt agaagccggc acccaggtcc tccatcatct ggatggcctc
     1861 cagccgcttc ttcgcgttgg cgttgagggc gtcctcgggc ggggtgagga ggtaggcgta
     1921 ggcctggtgg tgcgaccggc ccacgcggcc acgcaactgg tgcagctgcg cgagcccgaa
     1981 gcggtcggcg cggtcgatga ggatcgtgtt ggccgtgggc acgtcgattc cggtctcgat
     2041 gatcgtcgag cacagcagga cgttggcgcg ctggtggtag aagtcgcgca tcacccgttc
     2101 gaggtcgcgc tcgcgcatct gcccgtgtgc caccgcgatt cgcgcttcgg gcaggagctt
     2161 ttccagcgcc tcgcgccggt tctcgatcgt gtccacctcg ttccagaggt aataggcctg
     2221 gccgccgcgc ttgagctcgc gcagcaccgc ctcgcgcacg atgccgggcg attgacgggc
     2281 gacgaaggtc ctgatggcaa ggcgccgctg gggggccgtg gcgatgacgg aaaaatcgcg
     2341 caggccctcc agggacatcg ccagcgtgcg cgggatcggc gtggcggtga gcgtgagcac
     2401 gtcgacctcg gcgcgcaggc gcttgagctg ctccttctgg cggacgccga agcgatgttc
     2461 ctcgtcgatg atgacgaggc ccaggttctt gaatatgaca tccttctgga tcagcttgtg
     2521 cgtgccgatg gcgatgtcga tggagccatc ggcgagcccg gcgatcgctg cggcggtctc
     2581 cttcgggctg cggaaccggg agagctccgc gagcttcacg ggccactgcg cgaagcggtc
     2641 gctgaagttc tcgaagtgct gttccgccag aagcgtcgtg ggaacgagga tcgccacctg
     2701 cttgcccgcg gccacggcaa cataggccgc gcgcatggcg acctcggtct tgccgaatcc
     2761 cacgtcgccg cacacgaggc gatccatcgg ctttccgctc gtcatgtcgg ccaggacgga
     2821 ctggatggcg cccgcctggt cgggcgtctc ctcgaactcg aaggcgcccg agaacgcctc
     2881 gtagtcctgc gccttcagcg ggaatgcgta gccctggcga gcggcgcggc gcgcgtaaag
     2941 gtcgaggagc tcggcggcgg tgtcgcgcac ctgctgggcg gccttccggc gcgccttctc
     3001 ccactggccg ctgccgaggc gatgcacggg cgcgtcctcg ggcgaggcgc cggtgtagcg
     3061 gctgatcacg tgcagctgcg cgacgggcac gtagagcttg tcgccgccct cgtactcgag
     3121 gtggaggaat tcggttggcc cctcgccaag gtccatggtc acgagcccct ggtagcgccc
     3181 gatgccgtgc tggctgtgca cgacgggatc gccctgtttc acctcggcga gatcgcggag
     3241 catcccctcg gcgctggtga ttccgcgcgc atcgcggcgg cgcgactggc gcacctggga
     3301 ggggtagagc tccgcctcgg tgatgatggc gaagcgttcg cccggcgcgg cgaatccggc
     3361 ggcgagcggg ccgtgcgtga gcatgaaagg cgccggcgat tgcgtgaacc gcgtccagtc
     3421 gtccacgagc gccggatgca ggccgtgctc gccgaagagc tgcgcgagcg tctcgcggcg
     3481 gcccggtccg tcggcgacga cgaggacgcg cccgtcgaag gaagacacga accggtcgag
     3541 cgcgcgcaac ggctccgcgg aacgccggtc cacgccgaca tccggcagat tggccgcgac
     3601 gaataccggc gcgtttgccg gggcgggccc ggtggcatca cgcctgatgt ccaggcgagg
     3661 cagcccctgc aggccgacga agaattcctc gaccggaaga tagagctgct ccggaggcag
     3721 cagcggccgg tcgccatcgc cgcgcagcag ttcccagcga cccttcaggt cgcgccagaa
     3781 tgcctccgcg ccggccggaa cgtcttcgtg caggacgcag gtcgcgcccg gcggcaggta
     3841 gtcgaagaag gtcgccgtgg actcgaagaa gaggggcagg tagcactcga cgcccgcggg
     3901 ggcgatgccg ttcgagacgt ccttgtaggc gcggcgcttg gtcgggtcgc cctcgaagcg
     3961 ttcccggaaa ttggcccgga atcgcgcgcg cccctcttcg tccatcggga actcgcgcgc
     4021 cggcagcagg cgtacctcgg agacgggata gatggagcgc tgggtgtcga cgtcgaacgt
     4081 gcggatcgac tcgatctcct cgccgaagag atcgacgcgg tagggcaccg gactgcccgt
     4141 cggaaagagg tcgacgaggc cgccgcggat gcagaattcc ccgggcgcca tcacctgctg
     4201 gactgccgca tagccggcaa gcgccaactg ggccttgagc gcctcgaggt cgagtcgctc
     4261 cttcagccgg aggaagaacg ttcgcgcggc aaggtagctg cgcggcgcga gcctttgcag
     4321 cgccgtcgtg accggtatca ggccgacgtc gcaggctccc ttcgagaact ggtgcagggt
     4381 cgccagccgc tcggagacga ggtcggggtg cggcgagaac tggtcgtagg gaagcgtctc
     4441 ccagtcaggc agtcggtgca cggcgagcgc cgggtcgaac caggccacct cgtcgcgcag
     4501 gcgttcggcg tcctgcgccg actcggtcac cacgaagacc ggtttcgcgc ttctcgcgag
     4561 gcgcgcgatc cagaaggcgt cggaggagcc ggaacgggcg aacgcggacc ttgccacctc
     4621 gcctaggcga cgacgaccgg gatcttgccg atcttcgcgc gccactccgc cgggccggtc
     4681 ctgtgcacgg cgttgccgcc ggcgtcgacg gccaccgtta ccggcatgtc cttcacctcg
     4741 aactcgtgga tcgcctccat gcccaggtca tcgaaggcga ggacgcgagc cttcttgatc
     4801 gccttcgaca cgaggtaggc cgcgccgccc acggcgatgc agtaggccgc gccatgcttc
     4861 ttgatggact cgatcgcgat cgcgccgcgc tcgctcttgc ccaccatgcc gatgaggccg
     4921 gtatttgcga gcatcggttc gacgaacttg tccatgcgcg aactggtggt cggtcccgcg
     4981 gggcccaccg cttcctcgcg cacggcattg accgggccga cgtaatagat gaagcggttg
     5041 gtgaagtcga ccgggagctt ctcgccccgg gccatcatgt ccaccatgcg cttgtgggcg
     5101 gcgtcgcggc cggtgagaag cttgccggaa aggagcagcg tctcgccgga tttccaggtc
     5161 gcgagatcgg ccctggtgag cgtgtcgagg ttcacgcggc ggctcttcgc gaagtcgagg
     5221 gcgagcttcg gccactcgga gaggtccggc gggtcgaagg tggccggccc ggagccgtcg
     5281 agcacgaagt gctggtggcg cgtggcggca cagttgggca gcagcgccac gggcttggag
     5341 gcggcgtgcg tggggtaatc gttgaccttc acgtcgagca cggtggtgag gccgcccagc
     5401 ccctgcgcgc cgatcccgag cgcgttcacc ttctcgtaga gttccaggcg cagttcctcg
     5461 gcgcgcgtcc tgggcccgcg ctcgacgagg tcctggatgt tgatcgggtc catcattccc
     5521 catttggcca tcagcatcgc cttctccggc gtgccgccga tgccgagcga gaggatgccg
     5581 ggcgggcacc agtccgcgcc catcgtgggc acgatggaga gcacccagtc gacgatggaa
     5641 tccgaaggaa gcagcatcgc gaacttgctc ttgttctcgg agccgccgcc tttcgcggcg
     5701 acgatcacgt cgaccttgtc gccgggaacc agctcgacgt gaacgacggc gggcgtgttg
     5761 tcgccggtgc tcttcctttt cccgtccggg tcggagagaa cggaggcgcg caggggattg
     5821 tccttgtccg tgtaggcgcg acgcacgcct tcgtcgacca tctgctggag ggtcagcttc
     5881 gcgtcccagc gcacgtccat gccgacgcgg atgaaggcga tggcgatgcc ggtgtcctgg
     5941 cacatcgggc ggtggccctc ggcggacatg cgcgagttga tgaggatctg cgccatggca
     6001 tcccgggccg cgggcgactg ctcgagttcg taggcccggg ccagcgcgcg gatgtagtcg
     6061 accgggtggt agaagctgac gtactggaag gcgtcggcga cgctctggat caggtcatcc
     6121 tggcggatgg tggtcatggc gggtgaaggc gccctgcgtg cggcggcccg ttcatcgtgg
     6181 gggaccgtca ttctagcgca acggaccggt gcgcccgcag gggcgcaagg tcccgtgcga
     6241 ccctgcggcg ccccttgcca cgcgacgcat ccgggctagt gctcgttggc gaccggggac
     6301 ggcatgatgc ggtcgcaggc ggctatcgcc atcgccgaaa acaggaacgc gatgtggatc
     6361 cccgtctgcg cgatcagcac cttctcgctg tagttgtcgg cgttgatgaa ggtcttgagc
     6421 aggtggatgg aggagatgcc gatgatcgcg gtggccagct tcaccttgag aacggaggca
     6481 ttcacgtgcg agagccactc cggctggtcg gggtggtctt ccaggtccag gcgcgacacg
     6541 aaggtctcgt agccgccgac gatcaccatg atgagcaggt tcgagatcat caccacgtcg
     6601 atgagcgcca gcacgaccag catgatcacc gtctcgttga acttcggtgc cgcgtgtccg
     6661 acaacggcgc ccgacgcgtc cctgatctcc ggcaggctgt agccgacgct ttcgatgagg
     6721 gccgcgagcg ccgccttgtt gccgaaggcg gcctcgacca ggtgcaccag ttccacccag
     6781 aaatggacga cgtaaaccgc ctgcgcgacg atgagcccga ggtagagggg cgcctgcagc
     6841 cagcgggtcc agaaaatgaa accgcccagg tagcgaaggg cggcgggtcg gggaaggatg
     6901 ggcgtggaca tttcgcgatt ctagccggac gaccgcgcca gcgcgatggc gggcgtgatc
     6961 tcgcggcgca ggatcttgcc gtttcgcgtg cgcgggaatt ccctggcaag gtagacgacc
     7021 ttgggcgcct tgtaggcggc caggtgctcg cgcccgaacg cggcgaggtc atcgggcgtc
     7081 acggatttcc cggcatgctc gatcacgtag gcgacgacga ggagcttgtc cttgcccgcc
     7141 tcctcgccca cgcacgcgca gtcggcgacc gccgggtggc tcttcagcac ccgctccacc
     7201 tcgtaggggg agacgcggta gccgaagctc ttgatgatgt cgtccttgcg gccgaggaac
     7261 cacaggtagc cctcggcgtc gtatcgcgcg tagtcgccgg tgaggaacca gccgccgtgc
     7321 ctgagccgcg cggtttcctc gggaaggttc cagtagcgca ggaacaggcc cggatcggac
     7381 tcgggaacgc agatcattcc ctcctccccg gcggcgactt cgcggagcgt ttcgggatcg
     7441 agcagccgga tttcgtgtcc gggctgcgga aaacccgcgc tgcccggccg gatgggtcgc
     7501 cgcttgctct gcgagaggta gtagctgaac tcgctcatgc ccaccgcctc gaagatgtcg
     7561 agcccgaagc gatccttcca cagcccgaac acctcgtcgg aaaggtgctc gcccgcgctc
     7621 atgcagtggc gcagcgtcgg cacgtccgcc ctggtgaacg ccgtcttctg gagaatctgg
     7681 cggtatatgg tcggcacgcc gatgaagatc gtcgcgccat gcttggcgat gaggcgcggc
     7741 cacgtctcgg cgtcgttctt cccctcgtgc gcgatgacgg tcttgccgtg gtaaagcggg
     7801 tccatgagcg cggagcccag gacgtaggtc cagttgaact tgccggaatg gacgatgcgg
     7861 tccgtccggt ccgtcgcgcc gctgccttcc gggccgcgaa aatcgaacca gtaggaagcc
     7921 gccggggtgc gcccgatgag cgaacggtgg ccgtgcagga cgcccttggg atagcccgtg
     7981 gtgcccgacg tgtagacgag gtaggcgggg tcgtcgacgg cggtgtcctc ggccggctcc
     8041 cacgtggcga tccctgccag cgccgcctcg aggtcgatca cctccacccc cttgcagggc
     8101 gtcacctcgc ccgcgccgga caacagcgcg aagcgcagcg actcctttcc cgcgagccgg
     8161 gtgcccatcg ccttccaggc ggccttgtcg atgacgatgg cctgcgcgcc ggaatcgtgc
     8221 gcgaggtatt cgacttcctc tgccgtgagc agcgtcgagg tcggcacgct taccgccccg
     8281 cgcttcatcg cgccgaggaa cgcggtcgga taatcgatgc aattgggcag ccggatcagc
     8341 acgcgttcgc ccgcgccgat gcagaggtga cggagaagct ccgcgaaccg gctggtgcgc
     8401 tcggcgagcg cgcggtaggt gatggccctg gtgccgagcg catcgtcctc gacgatcatc
     8461 gccacgcgat cggccgccgg cgtgcccagg tgcgcgtcgg tgcaggcaac gccgatgttg
     8521 aggcgcgcgg gcacctccca cttccagggc ggaaagggcg cgaagggggg cgcggcgcgg
     8581 gcgttcacag gatgactccg tagggccagt tctcccgcac cacctgcgcg ggcaggcgct
     8641 tgagcacgtc catggcgaag tcggtgtcgg aaggcgagag cgcgtgcagg gcatgggcgc
     8701 gaagcagcgt ctgcagcgcc ttgccgaaca tcggcggatc gagcatctcg ccctggaact
     8761 tgatggcgcc gccgagcagg gcgtcggcct cgaccgcagc acgcaggatc gcgatcttgg
     8821 tcctgatctc ggacggggag ggcgtgaagg ccaccttgca cggatggatg tgcgaggggt
     8881 ggatcacctg cttgccggaa aggcccaggg aggcctcctc gcaggcgtgc cggtagacca
     8941 cgcgcgactc ctcgtcgccg tgcaggtcca gccagcggcg cacgtcgtcg ggctcgacga
     9001 agttctccgg catggtcgac tgcccgatga gtgtctcgac gccgccgatc acgcccttgc
     9061 cggcgattcg cgcctcgaag aggatgtcgt gcaggtagaa cttgagttcc tcggtccagt
     9121 ggcgcggcgt gatgtggatg cccatcgcct tggagaagtc gtggatgccg aacaccacgt
     9181 gcttcaccgt cgcgtactgc atgagttcgg gcgcgatctt gagcgacttg ggatgctcga
     9241 tgatcggctg gatcgtgatg ccgtgattga gtcccacgag gacggcggac aggtcgcgga
     9301 tctccggggc gccataggcc tcgcccgcct tcgcgagcat caccacgtcg aagtgatcgc
     9361 agaggtcgcg aacgagcgcg aggtccttct cgtattcctc cgtgcggaac gggttgatgc
     9421 gcaccgcgat ctggacctcg cgcttgcgcg gcagcccggg aagttcccgc ctcagcagtt
     9481 cccggctcat ttccttctgc cggcagccgt cctcgaggtc gaagagaagg gtgtgcgacg
     9541 tcgtgttcca cgcgtgcttc ttgaagcgct gcgccgcctg ctcgaggtcc tcgtagatgc
     9601 ccagggaagg cgcgaacttg accggcggat agtagatctg gatgccgggc cagtaatcgc
     9661 ccaggatctc gggcgcggcc gcggcggagg cgggcgaggg ggcgtgctgc gggacgttca
     9721 tggaatcctc gaaagttcaa cgggttgcac gtcacggcgg cgcccgatgc ggcaaccctg
     9781 tcggcgcagg aacccgtgcg cggcccacgc tagcaccgat gttgcggtgc gtcaacagtc
     9841 ttatataaga taggagactc gcgcccgggc ttcagggttt tcccgggggg gaggggcgcg
     9901 gcgcgcgcct tgctgtgcta taaaaacgcc ggccggccgc ggatcggggc gcgtcggaag
     9961 atgccgtcaa gcaggaatcg ccaacaggag gaataccggc aatgtcgaag atcgaatccg
    10021 tccaccagga gacgcgcgtc ttcccgccgt cggaagcgtt cgtgaagcag gccaatgtct
    10081 ctggcatggc cgcctacaag gccctgtgcg acgaggccga gcgcgatttc gagggcttct
    10141 gggcgcggct cgcgcgcgag aacctcttgt ggtcgacgcc gttcacgaag acgctcgacg
    10201 agtcgaacgc cccgttcttc aagtggttcc acgacggcga gctcaacgcc tcctacaact
    10261 gcctcgaccg gcacctgaag acgcagcccg acaaggtcgc gatcatcttc gaggccgacg
    10321 acgggaaggt gaccaaggtc acctacaagg agctctacca ccgcgtctgc cagttcgcca
    10381 acgcgctcaa gtcgcacggc atcaggaagg gcgagcgcgt gctcatctac atgccgatgt
    10441 cgatcgaggc ggtggtcgcg atgcaggcct gcgcgcggat cggcgccacg cattccgtgg
    10501 tcttcggcgg gttctccgcc aagagcctgc atgaacgcat catcgacgcg ggcgccgtgg
    10561 cggtcatctg cgccgatggc cagttccgcg ccggcaagga gatcgccctc aagcccgcgg
    10621 tcgacgaggc gcttgccatg ggcggctgcg aaaccattcg cgaggtcatc gtctacaagc
    10681 gctccggttc ccccgtcgaa atgcaggccc cgcgcgacaa gtggtggcac gacgtggtcc
    10741 agggccaggc gcaggactgc gagccgacct gggtgaacgc cgagcacccg ctcttcatcc
    10801 tctatacctc cgggtccacg ggcaagccca agggcgtgca gcactcgacg ggcggctacc
    10861 tgctgctcgc ggccctcacg atgaagtggg tgttcgacaa caagcccacc gacgtcttct
    10921 ggtgcaccgc ggacgtgggc tgggtgacgg gccacaccta catcgcctac gggccgctgg
    10981 cggtcggcgc gaccgaggtc gtcttcgagg gcgtgcccac ctacccggac gccgggcgct
    11041 tctggaagat gatccaggac cacaaggtca acgtcttcta cacggcgccg acggccatcc
    11101 gctccctcat caaggccggc cacgacctgc cgacgaagta cgacctgtcg tcgctgcgca
    11161 tcctcggcac cgtgggcgaa cccatcaatc ccgaggcgtg gatgtggtac taccagacgg
    11221 tgggcggctc gcgctgcccc gtggtggaca cgttctggca gaccgagacg ggcgcgcaca
    11281 tgatcacgcc gctgccgggc gccacgccgc tcgttcccgg ttcctgcacg ctgcccctgc
    11341 cggggatcat ggcggcgatc gtcgacgaga cgggccagga actgcccaac gggcacggtg
    11401 gcatcctcgt cgtcaggcgt ccctggccgt ccatgatccg cacgatctgg ggcgatcccg
    11461 agcgctaccg gaagagctac taccccgagg agctgggcgg caagctctat ctcgcgggcg
    11521 acgggtccat ccgcgacaag gagaccggct atttcacgat catgggacgc atcgacgacg
    11581 tgctgaacgt ctcggggcac cgcatgggca cgatggagat cgagtcggcg ctggtggcca
    11641 acaccaggct cgttgccgag gcggcggtcg tcggccggcc cgacgacatg accggggagg
    11701 ccgtggtggc gttcgtggtc ctcaagggcc accgcccgac gggcgacgcg gcgagggagc
    11761 tggcgaagga gcttcgcgac tgggtggcca aggagatcgg cccgatcgcc aagcccaagg
    11821 agatccgctt cggcgacaac ctgcccaaga cgcgctccgg caagatcatg cgccggctgc
    11881 tgcgcctgct cgccaagggc gaggagatca cgcaggatgt ctcgaccctg gagaacccgg
    11941 cgatcctcga gcagctcaag cacccgttgt aagcgagccc gcattgcgga cgcgaagacg
    12001 ggacgggatc ctatcccgtc ccgttttggt ttgacgggcg tcattcccgg caatcccgcg
    12061 catggaacac ttgccccatg aacgaacgcc agccgccgcc gctcctcacc gccgccaccg
    12121 ccgacgtgct gcgcaagcac gccccgttcg acgccatgcg cgaggccgac ctcgcgcacc
    12181 tcgcggcggg cctgaagctg cgctatttcg cgaaggacac ggtgatcctg tcgccggcga
    12241 gcgggcgcgt cacgtcgctc ttcatcatcc agcgcggcgt ggtgcaggcc gacgagccct
    12301 cgcaggtctc gctcaacacg atggccgcca ccctgaccga cggcgaagtc tttcccgtcg
    12361 gggccctcat cgcgaatcgc cccacgacgc tgcgctacaa ggcactcagg gacacgttct
    12421 gctatctgct cgacgaagtg cgcttcaagg aagtgatggc ccgcagccac gagttccacg
    12481 ccttctgcac gcgcaggctg gcggcgcttc tcgccacctc cacccgcggc atgcgcgagg
    12541 cctactcgca ccgggccgcc gccgagctca ccatggcctc gcccctgaga aatgcgttgc
    12601 gccacgagcc ggtcacgctt cccgagacgg ccagcgtgcg ccaggtgctc gagctgatga
    12661 agtcgcgccg catcggttcc atcgtcctca ccgggagcgc cggggggccg tcgggcatct
    12721 tcacgcagac cgatgtcctc aaccgcgtgg cgctcgcggg acagccgctc gaccggccgg
    12781 tgtcggaggt gatgacgcgc gggccggccg ccctcgcggc gggaagcgcg ctttcggaag
    12841 cggcgcaact catggcgagc cgcgggtttc gccacgtgct ggtcatcgat ggcgaccggc
    12901 tcctcggggt gatttccgag cgcgacctgt tcgggctcca gcgcctgtcc ctgcaggggc
    12961 tgcgcaagga catcggcggc gccacgaacg tcgatgcgct cgccttcgcg gcccgcgagg
    13021 tccgcgagct cgggaccgcg atgctcgcgc agggcgtggc ggcggagcag ctcacgcagc
    13081 tcgtctcggc cctcaacgac gcgataacgg gacgcgccat cgaactcgcg cttcgcgacc
    13141 atgacgtcgc cgacaccggg tactgctgga tgggcctcgg cagcgagggt cgcaacgagc
    13201 agacgctcgc gaccgaccag gacaacgcca tcatcttccc ggacagggag ggccacgacc
    13261 gcgcggcgac gcgtgccagg ctcctcgcct tcgccgaaca ggtgaaccgc acgctggatg
    13321 cctgcggttt cccgttgtgc aagggcaaca tcatggcgcg caacccctcc tggtgcctga
    13381 gccttgccga gtggttcgat cgcttcgacg actggatccg caattccgac cccgaggcgc
    13441 tgctcaacgc gtcgatcttc ttcgatttcc gcgcgctcca cggggacgcg acgctggtcg
    13501 atcgcctgcg cgagttcctg ctcgcgaacg tgaaggagcg gccggtgttc ctgcgccaga
    13561 tggcggccaa tgccgtgctc acgcggccgc cgctgggcct gttcggcgat atcgtcggcg
    13621 gggaaggggg caccgtcgac ctgaagaggc aggcatcgcg cgtggtcgtc gactgcgcgc
    13681 gcatccttgc gctcgccgcc ggcgtcggcg cggtgtccac ggccgagcgg ctgcgcgcca
    13741 cgggcccgaa ggtgcgcatg agcgacgatg acgtcgcgac cgccatcgat gccttccagt
    13801 tcatccagat gctgaggttg cgcgcgcagg acagcctcga ccgcggcacg ggctcctccg
    13861 agcccaatcg gatcgacccc gattcgctga acagcctcga ccgccgcatc ctgcgcgagg
    13921 cgttgcgcca ggcgcgcaag ctccagaacc gcgtcgccct cgattaccag ctctgaggcc
    13981 tgggacgttg ctctcctcgc tctggccctt ccgccgcgca cgcgccggcc ttccggacga
    14041 ggtcgtgcgg cgcatcgagc actggcgcag cctcgccgat ttcgaacgcg gccgcccgcc
    14101 ttcctccggg cggtgggtcg tcgtggacgt ggagtcgagc ggcctggacg ccgtctccga
    14161 ctcgctcatc gccgtgggcg ccctggccgt cgtcgacggc gggatcgacc tcgcggacag
    14221 cttcgaggtc atcctgcgcc aggatgcccc gagcacggtg gacaacatcg aggtccacgg
    14281 catcggcggc atggaacaga ccgaaggtga agatcccggc gaggcgctgg ccgcctttct
    14341 cgccttcatc ggcaaggacc cgctcgtcgc cttccacgcc ccgttcgact cgacgatgct
    14401 gcgccgcgcc atcgaccggc acctcggcgt cgcat
//
LOCUS       DASUEV010000309         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1139881, whole genome shotgun sequence.
ACCESSION   DASUEV010000309 DASUEV010000000
VERSION     DASUEV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139881"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1020
                     /locus_tag="VFV55_09735"
     CDS             253..1020
                     /locus_tag="VFV55_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4944621.1"
                     /translation="MTRWIRFEHEGRAQFGTLEGETIRVHEGDMFVSPRATGASVPRA
                     AVKLLTPSLPTKMVALWNNYRALAEKLGQSVPPEPLWFLKANSSFHPGGQPIRVPVSY
                     AGKVIYEGELGIVIGKHSARISESDAPAHIFGYTCINDVTAVELLKKDATFDQWSRSK
                     SFDTFGVFGPVIATGLDPATLHVRTVLNGQERQNYPTADMFFPPARLVSLISQDLTLE
                     PGDVIACGTSVGVGSMKPGSDVVVSIEGIGELANRFE"
     gene            complement(1076..1573)
                     /locus_tag="VFV55_09740"
     CDS             complement(1076..1573)
                     /locus_tag="VFV55_09740"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX4944622.1"
                     /translation="MTPETVVFTVRYTKDHCLATAWRYWQRRLGYRYALELAIGSGLL
                     VLATQGPYRWLEIALMVAVGFFAILGAVIFFLHWHRALVGLRALDPPESTWTLTEEAI
                     AQKSSLGESAIGWEALIEVWRFDDLWILIWGRDVYSSIPSAQLPREARQMIERRAKVL
                     PARKS"
     gene            complement(1595..1951)
                     /locus_tag="VFV55_09745"
     CDS             complement(1595..1951)
                     /locus_tag="VFV55_09745"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX4944623.1"
                     /translation="MQTYHLAVMILGIGLAVGILHLVRRDHLYIRQGLFWIVVAAVSF
                     SFGVWPFLIDSLGELLGIAYPPTLLLLAAIIVLVVKALLGDIALTKLNRDLRRLNQRI
                     ALLEAEHPAKEPVSPE"
     gene            complement(1983..>2172)
                     /locus_tag="VFV55_09750"
     CDS             complement(1983..>2172)
                     /locus_tag="VFV55_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944624.1"
                     /translation="IGVLLLLRHANLRIAEIPVAMNRRVSGASRVFSSWWTVGRYMLE
                     TSILCLACWNRGAGARNR"
BASE COUNT          382 a          673 c          782 g          335 t
ORIGIN      
        1 cgaatggctg ctggtggcca tccgcgacgc gcagtcgcgc tggcatttca acccggcgcc
       61 ggagacgaag gtctcgccgg gcgatatcat cgtggccatc gtgagtccgg agggaaggcg
      121 gctgctcgag cgggcgctcg gggggccttc ggccgggtag ggcgggaaca cggatgaaca
      181 cggataagac cggatgaaca cggatgattt ccgatgtccg gatgaacgga ttgcatatca
      241 ggagatgagc gcatgacccg ctggatccga ttcgaacacg aggggcgcgc gcagttcggc
      301 acgctcgagg gcgagaccat ccgcgtccac gagggcgaca tgttcgtctc gccgcgcgcc
      361 accggggcga gcgtgccgcg cgccgccgtg aagctcctca cgcccagctt gccgacgaag
      421 atggtggcgc tgtggaacaa ctaccgcgcg ctcgcggaga agcttggcca gtcggtgccg
      481 ccggagccgc tgtggttcct caaggccaac agctcgttcc accccggcgg gcagcccatc
      541 cgggtgccgg tgtcgtatgc cggcaaggtg atctacgagg gcgagctggg catcgtgatc
      601 ggcaagcact cggcgcgcat ttccgagtcc gatgcgcccg cgcacatctt cggctacacc
      661 tgcatcaacg acgtgaccgc cgtggagttg ctcaagaagg acgcgacctt cgaccagtgg
      721 tcgcgctcga agagcttcga taccttcggc gtgttcgggc cggtcatcgc cacgggtctg
      781 gacccggcca ccctccacgt gcgcacggtg ctcaacggcc aggaacggca gaactatccg
      841 acggccgaca tgttcttccc gccggcgcgg ctggtttcgc tcatctcgca ggacctcacg
      901 ctggagccag gcgacgtcat cgcctgcgga acgtcggtgg gcgtgggctc gatgaagccg
      961 ggcagcgatg tcgtcgtgtc gatcgagggg atcggggagc tggccaaccg gttcgaatag
     1021 cggcatttgt acccggtaca tgtaccgggt acaaatgccg ctgcttcccg ccgagctacg
     1081 attttctcgc cggcagtacc ttggcccgcc gctcgatcat ctggcgcgcc tcgcgcggga
     1141 gctgggcgga cgggatcgag ctgtacacgt ccctgcccca gatgaggatc cacaggtcgt
     1201 cgaagcgcca cacttcgatg agcgcctccc agccgatggc gctctcgccc aggctgctct
     1261 tctgggcgat ggcttcctcg gtgagggtcc aggtactttc gggcggatcc agcgcgcgca
     1321 ggccaaccag cgcgcggtgc cagtggagga agaagatcac cgcgccgagg atcgcgaaga
     1381 agcccacggc caccatgagc gcgatctcca gccaccggta gggaccctgc gtggcgagga
     1441 cgaggagtcc cgacccgatc gcgagctcca gcgcgtagcg atagcccagg cgccgctgcc
     1501 agtagcgcca ggcggtcgcg aggcagtggt ccttcgtgta gcggacggtg aagacgacgg
     1561 tttcgggggt catgcgggga aagccggcgc ggcgctactc cggggaaacg ggttccttgg
     1621 cggggtgctc ggcttcgagc agggcgatgc gctggttcag gcggcgcagg tcgcggttca
     1681 gcttggtgag ggcgatgtcg ccgaggagcg ccttcaccac gagcacgatg atcgccgcga
     1741 gcaggagcag ggtgggcggg taggcaatcc cgagcagctc ccccagcgaa tcgatgagga
     1801 agggccagac cccgaacgaa aatgaaaccg cggccacgac aatccagaac aatccctggc
     1861 ggatatacag gtggtcgcgg cggacaaggt ggaggatgcc gaccgccagc ccgataccga
     1921 ggatcatgac ggcgaggtgg taggtctgca tgcgcgtgtg ccggtgggtg ggtgtgggga
     1981 gtctagcggt ttcgcgcgcc ggccccgcgg ttccagcagg cgaggcacag gatcgacgtc
     2041 tcgagcatgt agcggccgac cgtccaccag gaggagaaga cgcgcgaggc gccgctcacg
     2101 cgccggttca tggcgacggg gatttccgcg atgcgcaggt tcgcgtggcg caggagcagg
     2161 agcacgccga tg
//
LOCUS       DASUEV010000310         6821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_654270, whole genome shotgun sequence.
ACCESSION   DASUEV010000310 DASUEV010000000
VERSION     DASUEV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654270"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFV55_09755"
     CDS             complement(<1..377)
                     /locus_tag="VFV55_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944625.1"
                     /translation="MTKPTIALFTGDPAGIGPELVARLLADGSVAKRANVLLVGSREV
                     LEDGMRVARCRFDFVEGPGDAGGRDLERPVLVSSPLVEEGGFARGVVSARSGRHMLDG
                     LAHGIALCSGGHAQALCFAPLNKG"
     gene            complement(374..1894)
                     /locus_tag="VFV55_09760"
     CDS             complement(374..1894)
                     /locus_tag="VFV55_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4944626.1"
                     /translation="MEAFHALIHGFGIALTPFHLMLMVVGVLLGILVGVLPGLGAPNG
                     VTLLLPLTFTMEPVSAIILLSCMYWGALFGGSTTSILFNIPGEPSSVATTFDGYPMAQ
                     QGRAGEALTAAFTSAFFGALSGVLLITFLSTWVAEFAMRFSPPEFFGVYMLTFCSFIG
                     MGRNPPLKSLGSLLLGLACAAVGMDTVSGNLRLTYDITELLRGISFLVAVIGLFGIGE
                     ILSTMEEGLAFKGLASRINPRVVLQTWARLPRYWLTLLRSMAIGWWMGVTPGGPTAAS
                     FMSYGIAKKMSRRGEKFGYGEIEGVIAPETADHAAGTSALLPMLALGIPGSATAAVMM
                     GGLMIWGLKPGPMLFIEQKDFVWGLIASMYLGNVVSLAVVLATVPLFAAILRIPFSII
                     APVIIVVCAIGAYTVNNLVSDLGLMLVFGVLGYFFKKLDYPIAPMVLAMVLGDRAEEA
                     FRQSLLLSRGDLSIFWGSKISATLTVLSTLLILVPLLARMRRRLAGGPAPYSRSPS"
     gene            complement(1917..2384)
                     /locus_tag="VFV55_09765"
     CDS             complement(1917..2384)
                     /locus_tag="VFV55_09765"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4944627.1"
                     /translation="MGLFFVGAGALVVNGSLEQGVGWTSTGPDSGYFPFRIGWLIIAT
                     GLVNGVLAIAAAIRARRRGLRREIFLEHGKLKPVLQVFLPMVAYVVAIRWLGLYVSSA
                     LFIAAFMAAHGKYRWRALPTGIAISAVFYVLLEVWFKVELYKGPLVAWLLERL"
     gene            complement(2459..3481)
                     /locus_tag="VFV55_09770"
     CDS             complement(2459..3481)
                     /locus_tag="VFV55_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944628.1"
                     /translation="MTMHSNRPKKWTAQVLAMALALPVLAGASFAADWKPTKSVEFVV
                     PSGAGGGTDQFARLVQSIIVKHKLIDQSIVVTNKPGGAGAEGFVDVSLAKGDPHKVAF
                     GTNNAYLLPFVLKVPYAYEQLTPVVMMALDEFLIWVPADAPFDNPKAYLDAVKADPAK
                     YIMGGSQSKDTDQTLNELINKTYGIKLAYIPFKSGGEAATQLAGKHISSNVNNPNENI
                     SQWRAGQVKPLCVFARERMQYKEKVTATMSWGDIRTCRESGLPIDEYQMPRVIYMSSA
                     VKPEHLAFYAALFRKVSETPEWKAFLTNNALNGTFATGSEAKAYIARDTERAKVIFKD
                     AGWLIK"
     gene            complement(3540..4910)
                     /locus_tag="VFV55_09775"
     CDS             complement(3540..4910)
                     /locus_tag="VFV55_09775"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944629.1"
                     /translation="MNYELLFEELRHRRIRIAVTGANGGFGRTFLAQCHQVPQVELSV
                     LCDQDVEGLRATLLEAGFAESAFLVCASEADVRRAAGARRIALVADCALLPGAPHDIV
                     VEATGQPEASVAIALAAIERGAHVGMVTKETDSVVGPWLDGLARERGVVYTTVAGDQP
                     ANLIGLVTWARVLGFEVVAAGKSSEYDFVFHPRSNEVRQAGIARRVAVIAGLWQLGAD
                     VPAALERRSEALCDFPQSATPDYCEMNVVANATGLVPACDTLSFPACRTPELADVFVP
                     QEDGGILSRTGVVDVFNALRRDDEASFAGGVFVIVRCTNAGVWRLLEEKGHMVGRSGK
                     YACIYQPFHLMGLESLASVYSAVLHKRPSGGAAPASHAVMVARTLRDFRPGETLRMAG
                     HGHDIHDVSPRLLPATRARGIAPYYLAAGKRMKTGVPRGTDIPIDALELDGSALHAAW
                     RQMAGD"
     gene            complement(4999..>6821)
                     /locus_tag="VFV55_09780"
     CDS             complement(4999..>6821)
                     /locus_tag="VFV55_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4944630.1"
                     /translation="SECNQAIVRAEAEPALLQAVCRILVEPGGYRMAWVGYAEADGQR
                     RVWPMAMAGHETAYVERNKIGWADDDRGQGPAGKALRSGVPQVNRDSRSNPDMAPWRV
                     DALACGFLSSIAIPLKGETAPLGLLAIYAAELDAFDTAEVRLLNEVGADLAFGIAALR
                     VRSARQRAEEMVARLAYFDSLTGLPNRTQLLERMVRAITGGDGGNGEFALLTINVNRF
                     DDIQIGLGVELGDEALKQLGARLGAALDKGPFLARLGGDIFGVLLERCNADCARATAD
                     RILLSLAEPFPLAGILVEMQASVGAALYPAHGSNCSALLLRSNIAARQARNTGAGYAL
                     YTGITDAESPRQLALVSELRRAIGSQELVLHYQPKVDLRTGRVAGAEALVRWQHPERG
                     LLLPAEFIPAAERTGLIRPLTDHVIDSALGQTRRWSDRGLAVRVAVNISPSSLRDPEF
                     VRRVIALREKWGTEPASLQFEITESTLMEDPARSHDVLMQLRDCGISIFIDDFGTGYS
                     SLSYIATLPIHGLKIDRSFIAGMLSNAQVRTVIEATISLARTLEMQVVAEGVETREQA
                     EELARMGCDELQGFHFGRPMLPEELEELAAASSLARYGLA"
BASE COUNT         1154 a         2355 c         2284 g         1028 t
ORIGIN      
        1 cccttgttga gcggcgcgaa gcagagcgcc tgcgcgtggc cgccgctgca aagcgcgata
       61 ccgtgcgcga ggccatcgag catgtgcctt ccgctcctgg ccgagaccac gccgcgcgcg
      121 aagcccccct cctccacgag cggcgagctc accagcaccg ggcgctcgag gtcccgcccg
      181 cctgcgtcgc ccgggccctc cacgaagtcg aagcggcaac gcgcgacgcg catcccgtct
      241 tcgagcacct cgcggctgcc caccagcagg acattggccc gcttcgcgac gctgccgtcg
      301 gcgagcagcc tggcgaccag ttccggcccg atgccggcgg ggtcgcccgt gaaaagtgcg
      361 atggtgggct tcgtcatgac gggctccttg aatacggcgc cgggccaccg gcgagccgcc
      421 ggcgcatcct cgccaacagg ggcacgagga tgagcagcgt cgacagcacg gtgagtgtcg
      481 cggaaatctt cgagccccag aagatcgaca ggtcgccgcg cgacagcagg agcgactggc
      541 ggaaggcctc ctcggcccgg tcgccgagca ccatcgccag caccatcggc gcgatcgggt
      601 agtcgagttt cttgaagaag taccccagca cgccgaagac gagcatcagc ccgaggtcgg
      661 agaccaggtt gttcacggtg taggcaccga tcgcgcacac gacgatgatc accggggcga
      721 tgatgctgaa ggggatgcgc aggatggccg cgaagagcgg cacggtcgcg agcaccacgg
      781 ccaggctcac gacgttgccc aggtacatgc tggcgatgag cccccacacg aagtccttct
      841 gctcgatgaa gagcatcggg ccgggcttca gcccccagat catgaggccg cccatcatca
      901 cggcggcggt ggccgaaccg ggaattccca gcgccagcat cggcagcagc gcgctcgtgc
      961 ccgcggcgtg gtcggcagtc tccggcgcga tcacgccttc gatctcgccg taaccgaact
     1021 tctcgccgcg ccgcgacatc ttcttcgcga tgccgtagct catgaacgaa gcggcggtcg
     1081 gcccgccggg cgtgacaccc atccaccagc cgatggccat gctgcgcagg agcgtgagcc
     1141 agtagcgcgg aagcctggcc caggtctgca gcacgaccct cgggttgatt cgcgaggcca
     1201 ggcccttgaa ggcgaggccc tcctccatcg tggacaggat ttcgccgatg ccgaagagcc
     1261 cgatcaccgc cacgaggaag ctgatgccgc gcagcagctc cgtgatgtcg taggtgagcc
     1321 gcaggttgcc cgagacggtg tccatgccca cggccgcgca agccaggccc agcagcaggg
     1381 acccgagcga cttgagcggc ggattgcgcc ccatgccgat gaagctgcag aaggtcagca
     1441 tgtagacgcc gaaaaactcg ggcgggctga aacgcatcgc gaactccgcc acccacgtcg
     1501 aaaggaacgt gatgaggagc acgcccgaga gcgcgccgaa gaatgccgag gtgaacgccg
     1561 cggtgagggc ctcacccgcg cgcccctgct gggccatggg atagccgtcg aaggtcgtcg
     1621 ccaccgacga aggctcgccg ggaatgttga acaggatcga ggtggtcgaa ccgccgaaga
     1681 gcgcgcccca gtacatgcag gaaagcagga tgatcgccga caccggctcc atcgtgaagg
     1741 taagcggcag caacagcgtc acgccgttgg gcgccccaag tcccggcagc acgccgacga
     1801 ggatgccgag cagcacgccc acgaccatga gcatcaggtg gaacggcgtc agagcgatgc
     1861 cgaagccgtg gatcagggcg tggaaggctt ccattgtcgg cgccgggcgc gttgcgctac
     1921 agccgctcca gcagccatgc gaccagcggc cccttgtaga gctccacctt gaaccacacc
     1981 tcgagcagca cgtagaagac cgcggaaatg gcgatgcccg tcggcaacgc gcgccagcga
     2041 tactttccgt gcgccgccat gaaggcggcg atgaacagcg ccgacgacac gtacaggccc
     2101 agccagcgga tggccaccac gtaggccacc atcggcagga acacctgcag cacgggcttc
     2161 aatttgccgt gctcgaggaa gatctcgcgc cgcaagccgc ggcgcctggc gcggatcgcc
     2221 gcggcgatgg ccaggacccc attgacgagg cccgtggcga tgatgagcca gccgatccgg
     2281 aacgggaaat accccgaatc gggaccggtg ctggtccacc ccacgccctg ctcgaggctg
     2341 ccgttcacca ccagcgctcc cgcgccgacg aaaaacagcc ccatgccggc ttcagcggtc
     2401 cagcgggcga agccccgcgg ggcgcgcccg ccgtcctgcg gcggctgcgg gccggccgtc
     2461 acttgatgag ccagcccgcg tccttgaaga tcaccttggc acgctcggtg tccctggcga
     2521 tataggcctt ggcctcgcta cccgtcgcga aagtgccgtt gagcgcgttg ttggtgagga
     2581 acgccttcca ttccggcgtc tccgagacct tcctgaagag cgcggcgtag aaggcaaggt
     2641 gctcgggctt gacggccgac gacatgtaga tcacgcgagg catctggtat tcgtcgatgg
     2701 gcaggcccga ttcgcgacag gtccggatgt ctccccacga catcgtcgcg gtgaccttct
     2761 ccttgtactg catgcgctcc ctggcgaaga cgcaaagcgg cttcacctgg ccggcgcgcc
     2821 actgcgagat gttctcgttg gggttgttca cgttcgagct gatgtgcttg ccggcgagct
     2881 gggtcgcggc ttcgccgccg ctcttgaacg ggatataggc gagcttgatc ccgtaggtct
     2941 tgttgatgag ctcgttcagg gtctggtccg tgtccttgga ctgcgacccg cccatgatgt
     3001 atttcgcggg atccgccttc accgcgtcga ggtaggcctt ggggttgtcg aagggcgcgt
     3061 cggccggcac ccagatcagg aactcgtcca gcgccatcat caccacgggc gtgagctgct
     3121 cgtaggcgta gggcaccttg agcacgaacg ggagcaggta ggcgttgttg gtgccgaagg
     3181 ccaccttgtg cgggtcgccc ttggcaaggc tcacgtcgac gaaaccctcg gccccggcgc
     3241 cgccgggctt gttggtgacc acgatcgact ggtcgatgag cttgtgcttc acgatgatcg
     3301 actgcaccag gcgcgcaaac tggtcggtgc cgccgccggc cccggaggga accacgaatt
     3361 ccaccgactt cgtgggtttc cagtcggcgg cgaagctcgc cccggcaaga accggcaatg
     3421 ccaacgccat cgcaagcacc tgcgcggtcc acttcttcgg tcgattgctg tgcatcgtca
     3481 ttttgccctc ctgggcttcg tgggttgggc cggtcttcgt gggaccggtt gcaggttgct
     3541 caatcgccgg ccatctggcg ccaggccgcg tgcagcgccg aaccgtcgag ctcgagcgca
     3601 tcgatgggaa tgtcggtgcc ccgcggcacg cccgtcttca tgcgcttgcc ggccgcgagg
     3661 taataagggg cgattcctcg cgcccgggtg gcgggaagga ggcgcggcga aacgtcatga
     3721 atgtcgtggc cgtgccccgc catgcgcagg gtctcgccgg gccggaaatc gcgcagcgtc
     3781 ctcgcgacca tcaccgcgtg ggatgccggt gcggcgcctc ccgacgggcg cttgtgcagg
     3841 acggcggaat acacgcttgc gaggctttcc aggcccatca ggtggaaagg ctggtagatg
     3901 caggcatatt tgccgctgcg tccgaccatg tgccccttct cctcgaggag ccgccacacg
     3961 cccgcattgg tgcagcgcac gatcacgaag accccgcccg cgaagctcgc ctcgtcgtcg
     4021 cgccgcaggg cgttgaagac gtccacgacg ccggtgcgcg aaaggatccc gccgtcttcc
     4081 tgcggcacga agacgtccgc cagctcgggc gtccggcagg ccgggaagct cagggtatcg
     4141 cacgcgggga cgagccccgt agcgttggcc accacgttca tctcgcagta gtcgggcgtc
     4201 gcgctttgcg gaaaatcgca cagcgcttcg ctgcgccgct cgagcgctgc agggacatcc
     4261 gccccgagct gccacaggcc cgcaatgacc gcgaccctgc gcgcgatacc ggcctgcctc
     4321 acctcgttcg accgggggtg gaagacgaag tcgtattcgc tcgacttgcc cgcggcgacg
     4381 acctcgaaac cgagaacgcg cgcccaggtg acaaggccga tgaggttcgc gggctggtcg
     4441 ccggcaaccg tcgtgtagac cacgccccgc tcgcgcgcga ggccgtccag ccacggaccc
     4501 acgaccgaat ccgtctcctt ggtgaccatg ccgacgtgcg cgccgcgctc gatcgcggcc
     4561 agcgcgatcg ccacgctcgc ctcgggctgc ccggtggctt ccacgacgat atcgtggggc
     4621 gcgccgggga gcagggcgca gtcggcaacg agggcgatcc tgcgcgcgcc cgcggcacgc
     4681 cgcacgtcgg cttccgaggc acagaccagg aaggcgcttt ccgcgaaccc cgcttccagc
     4741 agggtcgcac gcaggccctc gacgtcctgg tcgcacagga cggacaactc cacctgcggc
     4801 acctggtggc attgggcgag aaaggtccgg ccgaatcccc cgttcgcgcc cgtcaccgcg
     4861 atgcgaatgc ggcgatggcg gagttcctcg aagagcagtt cgtaattcat gacagcgccg
     4921 acctcatgcg cgaggaagtg ccggctcggg gaaacgcccg tccggcatcg gggaagcaac
     4981 cccggattcc atcgcccgtc acgccagccc gtagcgcgcg aggctcgacg cggcggccag
     5041 ttcctcgagt tcttccggga gcatggggcg gccgaagtgg aagccctgca actcgtcgca
     5101 acccatgcgc gccagttcct cggcctgctc gcgcgtctcg acgccctcgg ccacgacctg
     5161 catctccagc gtgcgggcga gcgagatcgt cgcctcgatc accgtgcgca cctgcgcgtt
     5221 ggacagcatc ccggcgatga acgagcggtc gatcttgagg ccgtggatgg gcagcgtggc
     5281 gatgtagctg agcgacgagt aaccggtgcc gaaatcgtcg atgaagatcg agatgccgca
     5341 gtcgcgcaat tgcattagca cgtcgtggga ccttgccggg tcctccatca gggtggattc
     5401 cgtgatctcg aactggagcg aggccggttc cgttccccac ttttcccgca gggcgatgac
     5461 gcgacgcacg aactccgggt cgcgaaggct gctcggcgat atgttcaccg ccacgcgcac
     5521 ggcgagcccg cgatccgacc agcgacgcgt ctgcccgagg gcactgtcga tgacgtggtc
     5581 ggtgagcggc cggatgagcc cggtgcgctc ggccgccgga atgaactcgg ccgggagcag
     5641 caggccccgc tccgggtgct gccaccggac cagcgcctcg gctcccgcga cccgcccggt
     5701 gcgcaggtca accttgggct ggtagtgcag caccagttct tgcgagccga tggcgcgccg
     5761 caactcgctc acgagggcga gctggcgcgg gctctcggca tccgtgattc cggtgtacag
     5821 cgcgtatccg gcgccggtgt tgcgcgcctg gcgggccgcg atgttgctgc gcaggagcag
     5881 cgccgagcaa ttcgagccgt gcgcgggata aagcgccgct ccgacgctcg cctgcatttc
     5941 gacgaggatc ccggcaagcg ggaatggctc ggcgagcgaa agcaggatcc ggtcggccgt
     6001 cgcgcgggcg cagtcggcat tgcagcgttc gagcaacacg ccgaagatgt cgccgccgag
     6061 ccgcgccagg aacggcccct tgtccagcgc cgcgcccagc cgcgcgccca gctgcttcag
     6121 cgcctcgtca cccagttcga cgcccagtcc gatctggatg tcgtcgaaac gattcacgtt
     6181 gatcgtgaga agcgcgaact ccccgttgcc cccgtcgccg ccggtgatgg cccgcaccat
     6241 gcgctcgaga agctgcgttc ggttgggaag cccggtgagg gaatcgaaat aggccaggcg
     6301 cgcgaccatc tcctcggccc gctggcgcgc cgaacgtacg cgcagggcag cgatgccgaa
     6361 ggcgaggtcc gcccccactt cgttcagcag ccgcacctcg gccgtgtcga acgcatcgag
     6421 ctcggccgcg taaatggcca gcaagccaag gggagccgtc tcgcccttca ggggaatcgc
     6481 gatggacgac aggaacccgc aggccagggc gtccacgcgc cacggcgcca tgtccgggtt
     6541 cgagcgcgaa tcccggttca cctggggcac gccgctgcga agggcctttc cggcgggacc
     6601 ctggccgcga tcgtcgtccg cccagccgat cttgttgcgc tcgacgtacg ccgtctcgtg
     6661 tcccgccatc gccatcggcc acacgcgccg ttgtccgtcc gcttccgcgt aaccgaccca
     6721 ggccatgcgg tagccgccgg gctcgaccag gatcctgcac acggcctgga ggagcgccgg
     6781 ctcggcctcc gcccgcacga tcgcctggtt gcactcgctc c
//
LOCUS       DASUEV010000311         6995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1474341, whole genome shotgun sequence.
ACCESSION   DASUEV010000311 DASUEV010000000
VERSION     DASUEV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474341"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFV55_09785"
     CDS             complement(<1..661)
                     /locus_tag="VFV55_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX4944631.1"
                     /translation="MIRRYAALVLVTMVLAAACATRRPAPVTERAPPPPLPVPQAVQP
                     VSPPVVPAAKPVPTHTVRRGETLVGIALQYGLDYRELAAWNGIANPNLISVDQVLFLA
                     SPQGAPAGTPAIVATPLAQTGPIVEARPLANTDRLKVEPRGQRLPYSDKALAQVSAGE
                     AMAEPAPAVAPTPVPATAPPAAPAAPAAVPEPERPSAGTQGEAIDWIWPAKGKVLAGF
                     TE"
     gene            complement(674..1303)
                     /locus_tag="VFV55_09790"
     CDS             complement(674..1303)
                     /locus_tag="VFV55_09790"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX4944632.1"
                     /translation="MTSDRTRGRMVEALRKSGIADERVLAAMLEIPRHLFVEAGIASR
                     AYEDTPLPIGHGQTISSPTIVALMTQLLLDKGPVAKVLEIGTGCGYQTAVLARLVKEV
                     YTLERIAPLMDKARRNLRDLRIYNVRFKHADGHKGYPEAGPYDGILSAASASHVPEAL
                     KEQLAVGGRLVIPVGTDEQWLHVIERTPAGFTDEKREAVRFVPMLPGIA"
     gene            complement(1359..2102)
                     /gene="surE"
                     /locus_tag="VFV55_09795"
     CDS             complement(1359..2102)
                     /gene="surE"
                     /locus_tag="VFV55_09795"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX4944633.1"
                     /translation="MRILLSNDDGYFAPGLAALAEALEPLGSLTVVAPERDRSGASNS
                     LTLDRPLSLHRASNGYFYVNGTPTDCVHMAVTGLLDIEPDIVVSGINSGSNMGDDTLY
                     SGTVAAATEGYLLGVPAIAISLVGKVYANYATAARVARELVDRIRRSPPAEPVLLNVN
                     VPDLPYDQLAGMEVTRLGRRHKAQPVVKSVNPRGDTVYWVGSAGAAREAGPGTDFHAL
                     ERGAVSITPLQVDLTHAGQMGAISRWLEQ"
     gene            2213..4252
                     /locus_tag="VFV55_09800"
     CDS             2213..4252
                     /locus_tag="VFV55_09800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX4944634.1"
                     /translation="MNRLPSRFAFAFAVAAALPALSPAAMLDVAGLSKADSPCDDFYD
                     YANRHWLDATSIPDDRAGWGTFAIIDKRNKERLEALLATAAASNPYPPGSNRHKIVAY
                     YASGMDEAGIARAGLSPLKPLLAEIDAVSEPGDLPKALAALHRAGIRAGFAFVVRQDA
                     KDSRRYLAEIQQAGLGLPDRDYYFRDDARSTSQREAYREHLQSIFRLSGEAAPAAVAL
                     AGSVLATETQIARAHMTLVERRDPEKTYNLRTVEGLAREAPGFDWRGYFAAIGAPGLT
                     EVNVAQPQAAAAFAALAASRPAADWRAYLRWHALNAAATKLPREFEEAHFAFFQRTLS
                     GVKRPVARTERVIETISGRYGSEPLALALGELFVEDAFPPHARQRALELVGHVKAALR
                     DRLLALDWMEEATRRAALDKLERMAVKIGYPDRWRDFSAAQVGDRPYAQNWINANAFE
                     FARMLAHLGKPVDRGEWRMSPHIVNAYYGATLNEIVFPAGILQPPYFDPKADDAVNFG
                     GIGMVIGHEITHGFDDRGRRYDAEGNLREWWTPEDQKRYQERTRAIVAQYDAYPGVDG
                     LKVNGALTLGENIADLGGLRIAWLALQRALDGQPRANLEGFSPDQRFFLAFAQAWRSR
                     LRPEQERLRILTDSHSPPRYRVQGPLANLPEFARAFSCPANAKALRAGGERVNLW"
     gene            4266..5309
                     /locus_tag="VFV55_09805"
     CDS             4266..5309
                     /locus_tag="VFV55_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX4944635.1"
                     /translation="MNARAGGNGQDARARAEAFLAGACRATRVAIERWERLSGGAIQE
                     NVALDVVIEGGPHAGRHAWVLRTDSPSTVAVSHSRPREFEILRAAHGAGVRVPEPLFD
                     CHDGNGPDFFIMRRMPGIAAGHRLSKDDALVPDRAALARDLGENLARLHRVKPPVASL
                     AFLGEVPADHARATIAGYRHDLDQLTAVRGEAWPALEWGLAWCARHAPAPLAPCLLHR
                     DYRTGNYLVHEGRLTAVLDWEFAGWGDPREDLGWMLARCWRFARPDRDAGGVGSADDF
                     LAGYADAGGRATAKEETRYWQAMAHLRWAVIALQQAERHRSGSQRSLELALTAHIVPE
                     LENEILELTEGTP"
     gene            5306..5701
                     /locus_tag="VFV55_09810"
     CDS             5306..5701
                     /locus_tag="VFV55_09810"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEX4944636.1"
                     /translation="MKVEPSAADLLDIARATILAQVAPSLPEAQRYAALMAANALAIA
                     ARDLAAQTTAAAEIARIVALLAGWTPAGDESTALREGTALLATRIRAGRYDEGEARQR
                     LLEHLRETTRARLAVSNPRAMAREPTTTR"
     gene            5698..6471
                     /locus_tag="VFV55_09815"
     CDS             5698..6471
                     /locus_tag="VFV55_09815"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX4944637.1"
                     /translation="MITDPLFDIRGKVALVTGASSGLGENFARSLASRGAVIVAAARR
                     ADRLDQLVSRIRAEGGVAHAISLDVADAASVEAAVRKAADLAGPLDILVNNAGIADTK
                     AAIELTEADWRRVLDTNLDGAWRMAQAVAKAMISAKRPGCIVNIASILGLRQATHLLA
                     YAAAKAALIQVTKALALEWARYGIRVNAIAPGYVITEMNREFFQSEAGLAVAKRVPQR
                     RIGSPRDLEGALLLLVSDAGAYMTGSVVVVDGGHVVNSL"
     gene            6481..>6995
                     /locus_tag="VFV55_09820"
     CDS             6481..>6995
                     /locus_tag="VFV55_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4944638.1"
                     /translation="MDFTLPAPVEEYRRRYRDFVRATVLPLDTDPASFDEHQNIRAGV
                     LAGVREKAKAAGLWAPQMPRERGGQGLPVVGMAACYEELNYSLFGPVSVNCAAPDDGN
                     MILLNKVGTPAQKERWLQPIVDGKVRSSFAMTEPHPGSGSDPSMMLTTATRTGDKWVV
                     RGRKWFITGAD"
BASE COUNT         1031 a         2538 c         2415 g         1011 t
ORIGIN      
        1 cctcggtgaa gccggcgagc accttgccct tcgccggcca gatccagtcg atcgcttcgc
       61 cctgggtacc cgcggacggc ctctcgggct cgggcactgc ggccggcgcg gccggtgctg
      121 ccggcggcgc ggtcgcggga accggggttg gcgcgacggc aggagcaggt tcggccatcg
      181 cctcacccgc cgacacttgc gccagcgcct tgtcggaata cggcaggcgc tggccacgcg
      241 gctcgacctt cagcctgtcc gtgttggcga gcggccgcgc ttcgacgatc ggcccggtct
      301 gggcgagcgg ggtggccacg atcgccggcg tgccggcggg cgcgccctgc ggagacgcaa
      361 gaaagagaac ctggtccacg ctgatgaggt tcgggttggc gatgccgttc caggcggcga
      421 gctcgcggta atcgagcccg tattgcagcg cgatgccgac cagcgtctcg ccccgcctga
      481 cggtatgggt ggggacgggc tttgccgcgg gcacgaccgg aggcgataca ggctgcaccg
      541 cctgcggcac gggcagcggg ggcgggggtg cgcgttccgt cacgggcgcg ggccgcctcg
      601 tggcgcaagc cgcggccagc accatcgtta ccaggacaag agcggcgtat cttcggatca
      661 tcggggcatt acctcaggcg attcccggca gcatcggaac gaagcgcacc gcctcgcgct
      721 tttcgtccgt gaagcccgcg ggcgtgcgct cgatcacgtg cagccactgc tcgtccgtgc
      781 ccacggggat cacgaggcgg ccgcccacgg cgagctgttc cttgagcgcc tccggcacgt
      841 ggctcgccga cgcagcggaa aggatgccgt cgtacgggcc tgcctcggga taacccttgt
      901 ggccgtcggc gtgcttgaag cgcacgttgt agatgcgcag gtcgcgaaga ttcctgcgcg
      961 ccttgtccat gagcggcgcg atgcgctcga gcgtgtagac ctccttcacc agccgggcaa
     1021 ggacggccgt ctggtagccg caacccgtgc cgatctcgag gaccttcgcc accggcccct
     1081 tgtcgagcag cagctgcgtc atgagcgcca cgatcgtcgg gctcgagatc gtctgcccgt
     1141 ggccgatggg cagcggcgtg tcctcgtagg cgcgcgaggc gatgccggcc tccacgaaca
     1201 ggtggcgcgg gatttcgagc atcgccgcga gcacgcgctc gtccgcgatt cccgacttgc
     1261 gaagcgcctc caccatccgg ccgcgcgtgc ggtcggatgt catgccaatg ccggcgggcg
     1321 tgctcaagtt cccgcccccg cgggcaagcc ggccgcgctc attgctcgag ccagcggctg
     1381 atcgcgccca tctgtcccgc gtgcgtcagg tccacctgca atggcgtgat ggacaccgcg
     1441 ccccgctcca gcgcgtggaa gtccgttccg gggcctgctt cgcgagcggc gccggccgaa
     1501 cccacccagt agaccgtgtc gccgcgcgga ttcacgctct tcacgaccgg ctgcgccttg
     1561 tggcggcgcc cgagccgcgt gacttccatg ccggcgagct ggtcgtaggg caggtcgggc
     1621 acgttgacat tgaggagcac cggctccgcg ggcggagagc ggcgaatgcg gtccaccagc
     1681 tcgcgcgcga cgcgcgccgc cgtggcgtag ttggcgtaaa ccttccccac cagcgagatc
     1741 gcgatggccg gcacgccgag caggtagcct tcggtggccg cggcgacggt ccccgagtag
     1801 agcgtgtcgt cgcccatgtt ggagccggag ttgattcccg agacgacgat gtccggctcg
     1861 atgtcgagga ggcccgtcac cgccatgtgc acgcaatcgg ttggcgtgcc gttcacgtag
     1921 aagtacccgt tgctggcgcg gtgcagggac aagggtcggt cgagcgtgag cgagttggac
     1981 gcgccgctgc ggtcgcgctc cggagccacg accgtgaggc tgccgagggg ctcgagcgcc
     2041 tcggcgagcg cagcgagacc gggtgcgaaa taaccgtcgt cgttcgaaag gagaatgcgc
     2101 atcgcggggg ctttcgcccg gggaatattg ctgcgcgatt atacccgatc gtccaccggc
     2161 cactcggcgc gcgtgggcta gaatgtcgcc tgcttcccgc gctctcccgc ccatgaaccg
     2221 actcccgtcg cgattcgcct tcgccttcgc cgtcgccgcc gcgcttcccg cgctttcccc
     2281 cgccgcgatg ctggatgtcg cggggctctc gaaggccgat tcgccctgcg acgacttcta
     2341 cgactacgcc aaccgccatt ggctcgatgc gacgtcgatc cccgacgacc gcgccggctg
     2401 gggcaccttc gccatcatcg acaagcgcaa caaggagcgg ctcgaggccc ttctcgcaac
     2461 ggccgccgcg tccaatccgt atccgccggg cagcaaccgg cacaagatcg tcgcgtacta
     2521 cgccagcggc atggacgagg cgggaatcgc ccgggcgggc ctctcccccc tgaagccgct
     2581 gctggcggag atcgatgccg tcagcgaacc gggggatctg ccgaaggcgc tcgccgcgct
     2641 gcaccgggcc ggcatccgcg caggattcgc gttcgtcgtg cggcaggacg cgaaggactc
     2701 gcgccgctat ctcgccgaga tccagcaggc cggtctcggc ttgccggacc gcgactacta
     2761 cttccgcgac gatgcgcgct cgacatccca gcgcgaggcc tatcgcgagc accttcagtc
     2821 catcttccgc ctgtcgggcg aggccgcgcc cgccgctgtc gccctcgccg ggagcgtgct
     2881 tgcaacggag acgcagatcg cccgcgccca catgacgctc gtcgaacgcc gcgaccccga
     2941 gaagacatac aacctgcgca ccgtcgaagg cctggcccgc gaggcacccg gcttcgactg
     3001 gcgcggctac ttcgccgcga tcggcgcccc cgggctcacg gaagtgaacg tcgcccagcc
     3061 ccaggccgcc gccgcattcg cggcgctcgc ggcatcgcgc cccgccgccg actggcgcgc
     3121 gtacctgcgt tggcacgcgc tcaacgccgc cgcgacgaaa ctgccgcgcg agttcgagga
     3181 ggcgcatttc gccttcttcc agcgcacgct ctcgggggtg aagcgtcccg tggcgcgcac
     3241 cgagcgcgtg atcgagacga tcagcgggcg ctacggcagc gagccgctgg cgcttgcgct
     3301 gggcgagctc ttcgtcgagg acgcgttccc gccgcacgcc cggcaacgcg ccctcgagct
     3361 ggtgggtcac gtgaaggcgg cgcttcgcga tcgcctcctc gccctcgact ggatggagga
     3421 ggccacgcgc agggcggcgc tcgacaagct cgagcgaatg gcggtgaaga tcggctaccc
     3481 ggaccgctgg cgggatttct cggccgcgca ggttggcgac cgcccgtatg cgcagaactg
     3541 gatcaacgcc aacgccttcg aattcgcccg catgctcgcg cacctcggca agcccgtgga
     3601 ccgcggcgag tggcggatgt cgccgcacat cgtgaacgcg tactacggcg cgaccctcaa
     3661 cgagatcgtc ttcccggccg gcatcctgca gccgccctac ttcgacccga aggccgacga
     3721 tgcggtcaac ttcggcggca tcggcatggt gatcggccac gagatcaccc acgggttcga
     3781 cgaccggggc cgccgctacg acgcggaggg caaccttcgc gagtggtgga cgccggagga
     3841 ccagaaacgc taccaggaac gcacccgggc gatcgtcgcg cagtacgacg cgtaccccgg
     3901 cgtggacggc ctgaaggtga acggggcgct cacgctcggc gagaacatcg ccgacctggg
     3961 cgggcttcgc atcgcctggc tcgcgctgca gcgcgctctc gacgggcagc cccgcgcgaa
     4021 cctggagggc ttctcgccgg accagcgctt cttccttgcg ttcgcccagg cgtggcgctc
     4081 gaggctgcgg cccgagcagg aacggctgcg catcctcacg gacagccatt cgcctccgcg
     4141 ctaccgcgtg cagggaccgc tcgccaacct cccggagttc gcgcgcgcgt tctcgtgccc
     4201 ggcgaacgcg aaggccttgc gcgccggcgg cgagcgcgtg aacctttggt gagcccgcgc
     4261 gcccgatgaa cgcacgcgcg ggcggcaacg gccaggatgc gcgtgcccgt gccgaggcct
     4321 tccttgccgg cgcctgccgc gccacgcgcg tcgccatcga gcgctgggag cggctttcgg
     4381 gcggcgccat ccaggaaaac gtggccctcg acgtcgtcat cgagggcggg ccgcacgcgg
     4441 gccggcacgc ctgggttctc cggacggact ctccgtccac cgtcgcggtg agccactcgc
     4501 gccctcgcga attcgagatc ctgcgcgccg cccacggcgc cggcgtgcgc gtccccgagc
     4561 cgctgttcga ctgccacgac gggaacggac ccgatttctt catcatgcgc cgcatgccgg
     4621 gcatcgccgc cgggcaccgc ctgtcgaagg acgatgccct ggtgcccgac cgcgccgcgc
     4681 tcgcacgcga tctcggcgag aacctggcgc gactgcaccg cgtgaagccg cccgtggcct
     4741 ccctggcctt cctgggcgag gtgcccgccg accacgcgcg cgcgacgatc gccgggtacc
     4801 ggcacgacct cgaccagctc accgccgttc gcggcgaagc ctggcccgcg ctcgaatggg
     4861 gcctcgcatg gtgcgcccgc catgccccgg caccactcgc gccctgcctc ctgcatcgcg
     4921 attaccggac cggcaactac ctcgtgcacg aaggccggct cacggccgtc ctcgactggg
     4981 aattcgcggg gtggggagac ccgcgcgagg acctcggctg gatgctggcg cgctgctggc
     5041 gcttcgcgcg ccccgaccgg gatgcgggcg gcgtcggttc cgcggatgat ttcctcgccg
     5101 gctacgcgga cgccggcgga cgcgcgacgg cgaaggagga gacacgctac tggcaggcga
     5161 tggcgcacct gcgctgggcc gtgattgcgt tgcagcaggc cgagcgccac cgctccggct
     5221 cgcagcgctc gctggaactc gccctcaccg cgcacatcgt cccggaactg gaaaacgaga
     5281 tcctcgaact cacggaaggc acgccatgaa ggtcgagccc tcggccgcgg atcttctcga
     5341 catcgcccgc gcaacgatcc tggcgcaggt cgcgccgtcc ctgccggaag cacagcgcta
     5401 tgcggcactc atggccgcca atgcgctggc catcgccgct cgcgacctgg ccgcgcagac
     5461 cacggccgcg gcggagatcg cgcgcatcgt ggcgctcctg gccggctgga cgcccgccgg
     5521 ggacgaatcg acggccctgc gcgaaggcac tgccctgttg gcaacccgca tccgcgcggg
     5581 ccggtacgac gaaggcgaag cccggcagcg gctgctcgag cacctgcgcg agaccacgcg
     5641 ggcgcggctc gccgtgagca accccagggc catggcccgc gagccgacga cgacccgatg
     5701 atcaccgatc ccctcttcga catccgcggc aaggtggcgc tggtcaccgg cgcctccagc
     5761 ggcctgggag aaaacttcgc acgatcgctc gcatcgcgtg gtgccgtgat cgtcgcagcg
     5821 gcccggcgcg ccgaccgcct ggaccaactc gtgtcccgga tcagggccga gggcggggtc
     5881 gcgcacgcga tatcgctcga cgtggccgac gcagccagcg tggaggcggc agtccggaag
     5941 gccgcggacc tcgccggtcc cctcgacata ctcgtgaaca atgccggcat cgcggacacg
     6001 aaggccgcca tcgaactcac cgaggccgac tggcggcgcg tgctcgacac gaatctcgac
     6061 ggcgcgtggc gcatggcgca ggcggtggca aaggcgatga tttccgcgaa gcgccccggc
     6121 tgcatcgtga acatcgcctc gatcctgggc ctgcgccagg ccacgcacct tctggcctat
     6181 gctgccgcca aggccgcgct tatccaggtg acgaaggcct tggcgctcga gtgggcgcgc
     6241 tacggcatcc gcgtcaacgc gatcgcgccc ggctacgtca tcaccgagat gaaccgggaa
     6301 ttcttccagt cggaggccgg gctggcggtg gcgaaacgcg tgccgcagcg gcgcatcggc
     6361 tcgccgcgcg atctcgaggg cgcgctcctc ctcctcgtct ccgatgccgg cgcctacatg
     6421 accggctccg tggtcgtcgt cgacggcggc catgtcgtga attctctcta ggacgccacc
     6481 atggacttca ccctgcccgc tccggtcgag gaataccgcc gccgctaccg cgatttcgtg
     6541 cgcgcgacgg tgctgccgct ggacaccgac ccggccagct tcgacgagca ccagaacatc
     6601 cgggccggcg tgctcgcagg cgtgcgcgag aaggcgaagg cagccgggtt gtgggccccg
     6661 cagatgcccc gggagcgcgg cggccagggc cttcccgtgg tcggcatggc cgcctgctac
     6721 gaggaactca actattcgct gttcgggccg gtgagcgtga actgcgccgc gcccgacgac
     6781 gggaacatga tcctgctgaa caaggtggga acgccggcgc agaaggagcg ctggctgcag
     6841 cccatcgtcg acgggaaggt gcgctcctcg ttcgccatga ccgaaccgca tcccggcagc
     6901 ggttccgacc cttccatgat gctcacgacc gcaacccgga ccggcgacaa gtgggtggtg
     6961 cgcgggcgca aatggttcat caccggcgcg gatga
//
LOCUS       DASUEV010000312         3717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1595749, whole genome shotgun sequence.
ACCESSION   DASUEV010000312 DASUEV010000000
VERSION     DASUEV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595749"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1611)
                     /locus_tag="VFV55_09825"
     CDS             complement(160..1611)
                     /locus_tag="VFV55_09825"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEX4944639.1"
                     /translation="MSAHISNVRPKPDKVLVDIADYVTKYKIKGSEAYETARYCLMDT
                     LGCGFEALEYPACTKLMGPIVPGTIVPNGAKVPGTPFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVAAGRKPLTVKDVLTGMIKAHEIQGCIAL
                     ENSFNKVGLDHVVLVKVASTAVVAEMLGLSRDEIINAISLAWVDGQSLRTYRHSPNTG
                     SRKSWAAGDATSRAVRLALIARTGEMGYPSVLTAKTWGFYDVLFKGDAFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTIHGELAKAGRSADDIERITIRTHEACIRIIDK
                     KGPLDNPADRDHCIQYMVAVPILFGRLTAQDYEDEVAGDPRIDKLRDRITCVEDPKFT
                     RDYHDPDKRSIANALTVEFRDGKKMKEIVVEYPIGHKRRRKEGMPVLVEKFRVNLARR
                     FPSKQQKAILELCLDQKKLEATPVNEFVDLMAV"
     gene            complement(1804..2967)
                     /gene="prpC"
                     /locus_tag="VFV55_09830"
     CDS             complement(1804..2967)
                     /gene="prpC"
                     /locus_tag="VFV55_09830"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX4944640.1"
                     /translation="MTEVATAPAVAPKPKKSVALSGVTAGNTELCTVGRTGNDLHYRG
                     YDILDLADKCEFEEIAHLLVHSKLPTAAELKAYKVKLRSLRGIPAAVQDVLECIPASA
                     HPMDVLRTACSALGSVLPEGDDHKLAPSRDIADRLMASFGSMLMYWYHFSHSGRRIEV
                     ETDDDSIGGHILHLLHGKKPPEPWVRAMHTSLILYAEHEFNASTFTARVVAGTGSDMF
                     SAITAAIGALKGPKHGGANEFAFDTIGRYDNPTEAEADIVKRIAEKQVIIGFGHPVYT
                     IADPRNQVIKEVARLLSKEAGNMKLFDISARIEEVMWREKKMFPNLDWYSATSYHMMG
                     VPTSMFTPLFVISRTSGWCAHVIEQRIDGKIIRPSANYTGPEDLKFVPLEKRK"
     gene            complement(3021..>3717)
                     /gene="prpB"
                     /locus_tag="VFV55_09835"
     CDS             complement(3021..>3717)
                     /gene="prpB"
                     /locus_tag="VFV55_09835"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEX4944641.1"
                     /translation="LDDVLTDIRRITDVCDLPLLVDVDTGFGASAFNVARTTKSLIKF
                     GAAAMHIEDQVGAKRCGHRPGKELVSLQEMADRVKSAADAKTDASFFIMARTDALAVE
                     GLQGAIDRACACVEAGADGIFPEAITDLDMYRKFAAAVKVPVLANITEFGKTPYFTVQ
                     ELASANVAVALYPLSAFRAMNKAALGVYQALRKEGSQKSVVDTMQTRDELYHYLGYHE
                     FEQKLDALFARTK"
BASE COUNT          557 a         1125 c         1305 g          730 t
ORIGIN      
        1 tcagctggta attcgtggcg cagccgccgg ctgcggccgc gatggcgccg gcaagcagca
       61 tccgggcggc cgctgtgatc gcgagtttcc tggtcatgtt tcctccatgc ggccggcggg
      121 gacttgcagc cctgttccgg cgcggtgtgt cgctcccctt cagacagcca tcaggtcgac
      181 gaactcgttg accggcgtgg cttccagctt cttctggtcg agacagagct cgagaatcgc
      241 cttctgctgc ttggacggga agcgccgggc gaggttcacc ctgaacttct ccaccagcac
      301 cggcatgccc tccttgcggc ggcgcttgtg gccgatgggg tactcgacca cgatctcctt
      361 catcttcttg ccgtcgcgga actccacggt gagcgcgttg gcgatcgagc gcttgtcggg
      421 atcgtggtag tcccgcgtga acttcgggtc ctcgacgcag gtgatgcgat cgcgcagctt
      481 gtcgatgcgc gggtcgccgg cgacctcgtc ctcgtagtcc tgtgcggtca gccggccgaa
      541 gaggatgggc accgccacca tgtactggat gcagtggtcg cggtcggccg ggttgtcgag
      601 cggccccttc ttgtcgatga tgcggatgca ggcctcgtgc gttcggatgg tgatgcgctc
      661 gatgtcgtcc gcgctcctgc ccgccttcgc gagctcgccg tggatcgtca tcgccgcctc
      721 caccgcggtc tgcgagtgga actcggcggg aaacgagatc ttgaacagca cgttctccat
      781 gacgtagctg ccgtaggggc gctggaacct gaacgcgtcg cccttgaaga gcacgtcgta
      841 gaagccccag gtcttggcgg tgagcacgga cggatagccc atctcgccgg tcctcgcgat
      901 gagggcgagg cgcacggcgc ggctggttgc atcccccgcg gcccagctct tgcgcgagcc
      961 cgtgttcggc gaatggcggt aggtgcgaag cgattggccg tcgacccacg ccagcgagat
     1021 cgcgttgatg atctcgtcgc gcgaaaggcc cagcatctcg gcgaccacgg cggtggaggc
     1081 caccttcacc agcaccacgt ggtcgaggcc caccttgttg aagctgttct cgagcgcgat
     1141 gcagccctgg atctcgtgcg ccttgatcat cccggtgagg acgtccttca ccgtgagcgg
     1201 cttcctgccc gcggcgacgg cgttgcgcga gagccagtcg gccgtggcga ggatgccgcc
     1261 gaggttatcc gacgggtggc cccattccgc cgcgagccag gtgtcgttga agtccagcca
     1321 gcggatcatg gcgccgatgt tgaacgcggc ctgcaccgga tcgagctgga agggcgtgcc
     1381 ggggaccttc gcgccgttgg gcacgatggt gccggggacg atggggccca tgagcttggt
     1441 gcacgcgggg tactcgaggg cctcgaagcc gcagccgagc gtgtccatca ggcagtagcg
     1501 cgcggtctcg taggcttcgc ttcccttgat cttgtacttc gtgacgtaat cggcgatgtc
     1561 gacgaggacc ttgtcgggct tgggacggac gttggagatg tgggcggaca ctttatggct
     1621 ttcctggcgg ggttaggtca aaggcagggg aaccacagat gaacacagat gaacacagat
     1681 ggtttatgac gaatgttgca tcggcgtact tgatgaacgt ccgtcaagaa cgcggatgat
     1741 tatccgatca caaattatct gtgttcatct gtgttcatct gtggttcccc gcctttcaac
     1801 gggttacttg cgcttctcga gcgggacgaa cttgaggtcc tcggggccgg tgtagttggc
     1861 gctgggacgg atgatcttgc cgtcgatgcg ctgctcgatc acgtgcgcgc accagccgga
     1921 ggtgcgcgag atcacgaaga gcggcgtgaa catcgacgtg ggcacgccca tcatgtggta
     1981 cgaggtcgcg ctgtaccagt cgaggttcgg gaacatcttc ttctcgcgcc acatcacttc
     2041 ctcgatgcgc gccgagatgt cgaagagctt catgttgccc gcttccttcg acagcaggcg
     2101 cgcgacctcc ttgatgacct ggttgcgcgg gtccgcgatc gtgtagacgg ggtggccgaa
     2161 gccgatgatc acctgcttct cggcgatgcg cttcacgatg tcggcttccg cttcggtcgg
     2221 gttgtcgtag cggccgatgg tgtcgaaggc gaactcgttg gcgccgccgt gcttcggtcc
     2281 cttgagcgcg ccgatggccg cggtgatggc cgagaacatg tccgagcccg tgcccgccac
     2341 gacgcgcgcg gtgaaggtgg aggcgttgaa ttcatgctcg gcgtagagga tgagcgaggt
     2401 gtgcatcgcg cgcacccacg gttccggcgg cttcttgccg tgcagcaggt gcaggatgtg
     2461 cccgccgatg gagtcgtcgt cggtctcgac ctcgatcctt cgtcccgagt gggagaagtg
     2521 gtaccagtac atgagcatcg agccgaacga ggccatcagg cggtcggcga tgtcgcgcga
     2581 gggggcgagc ttgtggtcgt ccccctcggg cagcaccgat ccgagggccg agcacgcggt
     2641 gcgcagcacg tccatcgggt gcgcggaggc cgggatgcac tccagcacgt cctgcacggc
     2701 ggccggtatg ccgcgcagcg aacgcagctt taccttgtag gccttgagct cggcggccgt
     2761 ggggagcttg ctgtgcacaa ggagatgggc gatctcctcg aactcgcact tgtcggcgag
     2821 gtcgaggatg tcgtaacccc ggtagtgcag gtcgttgccc gtgcgcccga cggtgcacag
     2881 ctcggtgttg ccggccgtga cgccggacag ggcgacggat ttcttcggtt tgggggcgac
     2941 ggcgggtgcg gtggcgactt cggtcatggc ggttccttcg gagggatgga cggaaggcgg
     3001 ctcgttggct cgagcgctcg ttacttggtc ctggcgaaca gggcgtcgag tttctgctcg
     3061 aactcgtggt agcccaggta gtggtacagc tcgtctcggg tctgcatcgt gtcgacgacg
     3121 gatttctgcg agccttcctt gcgcagcgcc tggtagacgc cgagcgcggc cttgttcatg
     3181 gcgcggaagg cggaaagcgg gtagagcgcg accgcgacgt tggccgaggc gagctcctgc
     3241 acggtgaagt acggcgtctt gccgaattcc gtgatgttgg cgaggaccgg caccttcacg
     3301 gcagcggcga acttgcggta catgtcgagg tccgtgatcg cctcggggaa gatgccgtcc
     3361 gcgccggcct ccacgcacgc gcaggcgcgg tcgatggctc cctgcagtcc ttcgacggcg
     3421 agcgcgtcgg tgcgcgccat gatgaagaac gaggcgtcgg tcttcgcgtc cgcggcgctc
     3481 ttcacgcggt ccgccatctc ctgcagcgag acgagctcct tgcccgggcg gtggccgcag
     3541 cgcttggcgc ccacctggtc ctcgatgtgc atggccgccg cgccgaactt gatgagcgac
     3601 ttcgtcgtgc gcgcgacgtt gaaggcgctc gcaccgaacc cggtgtcgac gtccaccagc
     3661 agcggcaggt cgcacacgtc ggtgatgcgg cggatgtccg tgaggacgtc gtccagg
//
LOCUS       DASUEV010000313         8498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1234067, whole genome shotgun sequence.
ACCESSION   DASUEV010000313 DASUEV010000000
VERSION     DASUEV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234067"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFV55_09840"
     CDS             <1..590
                     /locus_tag="VFV55_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX4944642.1"
                     /translation="CGTLSAWDRKYELTSRGAHLFREFFNTARNIANVFKVPFNYLDP
                     LNTPRGFNVTDPAIATAVAGALKAAGDKIRAAGLAIDSPLGQVQFTLRGTTPIPVHGG
                     EENLGIYNKVSSVLVPNLGYIPTTGSSYIQTVQFTDSGVNAQGFLTYSQSTNAASPHF
                     ADQTTRFMNKEWITFPFTDAQITSDPGYSTTTIRE"
     gene            698..2608
                     /locus_tag="VFV55_09845"
     CDS             698..2608
                     /locus_tag="VFV55_09845"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HEX4944643.1"
                     /translation="MKHAFRPMLKPLAIALALAGAGPALAVQFDMENGLTGSVDTTLS
                     YGISVRTGKRDPALIGIANGGTSRSVNEDDGDRNYDRNKAFANIAKATTDVELKYGRF
                     GFFGRGTAFYDFENANNDKLGPTAKDRVGKNVQGLDGFVSAAFEPMGKNLRLRAGRQV
                     ISWGESTFLPNGINVINPVDLTKLRIPGSELKEAFLPTTGLWANQELTKNASLEGFYL
                     TNHDKIRIDPRGTYFSNNDFASDDSDRVIITFGRRRDQTSPPSNPIPAATPQIGSVAS
                     ALYGPFDPAASIWAPRSPDRNPSDHGQWGLAMRYLAGELNNTEFGLYYMNYHSRIPLF
                     SGIKGTVTSVLTGGPLAAPTGQTGTATYFAEFPENIRLFGVSFNTAGPAGIALQGEYS
                     YRSNQPVQYSTPELLLAALGAPNLITGFTQIPGAPAGATAAALVPNGTYLQGYQRLKM
                     SQFQMTGTKSQPNVLGAEQLVIVGEAGVTYFHNLPTNVKFNGPAVYLPAAPLAALLPS
                     VAAFSNQTEGFLTSVSWGYRLVGRLEYNNALLGGNLSPRLAWSHDVNGVGPTFNQGIK
                     SLSIGASWEYQRKWLVDAQYTGYTGGRTYCGTDVPPTGSVVTPGQSANYCSSANPLKD
                     RDFFSVSVSYSF"
     gene            2679..4043
                     /locus_tag="VFV55_09850"
     CDS             2679..4043
                     /locus_tag="VFV55_09850"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HEX4944644.1"
                     /translation="MFAITRKATALIAVGAAFAIPAAAEMSAADVAKLGTTLTPMGAE
                     KAGNAAGTIPAWDGGITKPVAGYKAGGQYPDPFPADKPLFTIDGKNADQYKDQLAPGQ
                     MAMLKKYPTYKMIVYPSRRSAAFPTCHYNETKDCAAKARLAPGGNGVVGCTGGIPFPV
                     PKDGNEAIWNAELRYRGDTFAQHWAQAAVTRTGDYATVKFEYEYDFQYGNCSKPVAQR
                     EPNKILNYLQVTTAPARLAGQILLVHETVDQAKEPRSAWTYNPGQRRVRLAPNVAYDN
                     PGTAADGLRTNDDFGMYNGATDRYNWKLVGKKEMYMPYNSNRMTAPNLKYTDVLKAGH
                     LNQDLARYELHRVWVVEATLKPGTSHIYAKRVFYIDEDSWAILATDKYDGRGELWRFS
                     EQHNENWYNVPMFFGTIEVHNDLQSGRYIAMGLRNEENTIYKPISRSGADYAPNSLRA
                     LGTR"
     gene            4135..5094
                     /locus_tag="VFV55_09855"
     CDS             4135..5094
                     /locus_tag="VFV55_09855"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX4944645.1"
                     /translation="MSKVAGSLFAASLALATVPSRAADILPESGPMPRVTMERLLLVD
                     ATHRGSRVIAVGDHGYIVVSDDNGRTWRRAKSPAAPLLTAVDFLDAKSGWAVGHDSVI
                     LATADGGETWVQQFAAPGEQRPLLDVIFIDGNAGYAIGAYGAFYETTDGGKSWNPRKV
                     IGDDKHLNAFVRLPGGRLVILGEAGTILLSPDAGKTWEAVPSPYKGSLFGGLEADDGS
                     VIAFGLRGRIFRSTDAGKTWKAVDNPSVATLMGGSKLPDGALVIAGAGGTVLVSRDNG
                     LSFVPLATGSTKAYSKAVLGSANEVILFGESGVREVALPSAKR"
     gene            5103..7436
                     /locus_tag="VFV55_09860"
     CDS             5103..7436
                     /locus_tag="VFV55_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX4944646.1"
                     /translation="MSLDALIQSVSRFVFGQRRALLIVFTIATVLFAASASRLVVDAG
                     FNKMVPLKHPYMRVYRDYEKVFGGANRVAVAIVQKDGDIFNKEYMAKLKGLTDDVFLL
                     NGVDRPSVKSLFTPNTRFIEVIEEGFSGGNVIPATFQGTDDDLKQVRANVQKSSEIGR
                     TVATDFSGALVTAGLLEIDPQTNQRLNYFEVSARLEALRAKYEGPKHSVHIIGFAKAI
                     GDIRDGARGVLTFFAVAFLITAGLMLWFVKDVKLTGVALVVAMMPVLWLLGTLPILGY
                     GIDPLSILVPFLIFSIGVSHAVQMSKTWEREVVAGTDSLTAAKRAFARLFIPGTFALL
                     TNVLGFAVIMMIPIDIVRELGITASLGVAWMIITNKMLLPILLSYLQMAKTAATKEAY
                     QESRVERVWKAASGCVEKGRAAAIIAIALVILVAGIVGAHDLRVGDYGIGVPELRPDS
                     RYNRDNAKIIEKFAMGTNTLGVVAQTKGVQGACTNYDLMSVIERFDWFMQNAPGTQSV
                     ISLPGLAKNVNAGFNEGNVKWRQLPRDPQVMAQSVTPIDTATGLLNPDCSAMQVLVFT
                     TDQQGATIASLVSNVKAYAASNNSDKLEFKLATGNMGVTAATNEAVDSARWEMNFAIF
                     GALMVMCMITFRSLRATLCILLPLALVSVLCEALMPLLGIGLKVSTLPVIALGVGVGV
                     DYGIYLYDRIEVHLGEGKTLATSFFEALKERGTAMVFTAVTMTIGVGTWAMSALKFQA
                     DMGVLLAFMFFLNMLGAIFLLPALAAFLIKPHGAARG"
     gene            7446..7823
                     /locus_tag="VFV55_09865"
     CDS             7446..7823
                     /locus_tag="VFV55_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX4944647.1"
                     /translation="MAGLSRSLIAAGAVALATGVALGAIGAHAAKSAAHPDAARLIQT
                     AVFYQLVHGLGALIIGVLARGDGTSRLLALSGWLLLAGVVLFCGSLYALAFAALSLGP
                     VAPVGGTAFMTGWALLAVWAVRG"
     gene            8076..8264
                     /locus_tag="VFV55_09870"
     CDS             8076..8264
                     /locus_tag="VFV55_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944648.1"
                     /translation="MKRTIARITLALFASSLGSAFFVGDAFAKNSTSLGHGVKCTFVL
                     VKSVNGVNTYKTVCRRNH"
BASE COUNT         1516 a         2895 c         2692 g         1395 t
ORIGIN      
        1 aatgcggcac gctctcggcc tgggatcgca agtacgagct tacgagccgc ggcgcgcacc
       61 tgttccgcga gttcttcaac accgcccgca acatcgcgaa cgtcttcaag gttccgttca
      121 actacctcga tccgctcaac acgccgcgcg gcttcaacgt gacggacccc gcaatcgcca
      181 cggcggtcgc gggcgcactg aaggcggcgg gcgacaagat ccgggccgcg ggccttgcca
      241 tcgactcgcc ccttgggcag gtgcagttca ccttgcgcgg aaccacgccg atcccggtgc
      301 acggcggcga ggaaaacctc ggcatctaca acaaggtgag cagcgtgctc gtccccaacc
      361 tcgggtacat tcccacgacc ggcagcagct acatccagac ggtccagttc accgactcgg
      421 gcgtgaacgc gcaggggttc ctcacgtact cgcagtcaac gaacgcggct tcgccgcact
      481 ttgccgacca gacgacgcgt ttcatgaaca aggaatggat tacattccca ttcacggacg
      541 cgcagatcac atcggatccg ggctacagca cgacgaccat ccgggagtag gccggcgcgc
      601 attgtctttt ccctctcgat tcacgacaat agccgacagt ttgccgcctt cgtttgcagt
      661 gggcgcacga ataactaata tccaggagaa acgcctcatg aaacacgcct tccgtcccat
      721 gctgaagcca ttagccatcg cgctcgccct cgcgggcgcc ggcccggcgc tcgctgtcca
      781 gttcgacatg gaaaacgggc ttaccggcag cgtggacacc acgctttcgt acggcatctc
      841 ggtgcgcacg ggcaagcgcg atccggcgct catcggcatc gccaacggcg ggacgtcgcg
      901 ttccgtgaac gaggacgacg gcgaccgcaa ctacgaccgg aacaaggcgt tcgcgaacat
      961 cgccaaggcg acgacggacg tggagctcaa gtacggcagg ttcggcttct tcgggcgggg
     1021 cactgcgttc tacgacttcg agaacgcgaa caacgacaag ctcggcccca ccgccaagga
     1081 ccgtgtcggc aagaacgtgc agggtctcga cgggttcgtg agcgcggcct tcgagccgat
     1141 gggcaagaac ctgcgccttc gcgccggccg gcaggtgatc agctggggcg agagcacctt
     1201 cctccccaat ggcatcaacg tgatcaaccc ggtggacctc acgaagctgc gcatccccgg
     1261 ctccgagctc aaggaggcgt tccttcccac gacgggcctg tgggccaacc aggagctcac
     1321 caagaacgcg agcctggagg gcttctacct caccaaccac gacaagatcc gcatcgatcc
     1381 gcgcggcacc tacttctcga acaacgactt tgcctccgac gattccgacc gcgtgatcat
     1441 caccttcggc cggcgtcgcg accagacctc gccgccttcg aacccgatac cggcggcgac
     1501 gccccaaatc ggctcggtcg cctccgcgct ctacggacct ttcgaccctg cagcgtcgat
     1561 ctgggcaccg cgttccccgg accgcaatcc gagcgaccac gggcaatggg gcctcgcgat
     1621 gcgctacctt gccggcgaac tcaacaacac cgagttcggc ctctactaca tgaactacca
     1681 cagccgcatc ccgctcttct ccggcatcaa gggaacggtg acatcggttc tcacgggcgg
     1741 gcctctcgcg gcgccgaccg gccagaccgg gacggcgacc tatttcgccg agtttcccga
     1801 gaacatccgc ctgttcggcg tgagcttcaa cacggccggc ccggcgggaa tcgcgctgca
     1861 gggcgagtac tcgtaccgat ccaaccagcc cgtgcagtat tccacgccgg aactgctgct
     1921 cgccgcattg ggcgcgccca acctcatcac cggcttcacg cagattcccg gtgcgccggc
     1981 gggcgccact gcggccgcac tggtgcccaa tggaacctat ctccagggtt accagcggct
     2041 caagatgagc cagttccaga tgacgggcac caagagccaa cccaatgtcc tgggtgcgga
     2101 acagctggtc atcgtcggcg aagcgggcgt cacgtatttc cacaacctgc cgacgaacgt
     2161 gaaattcaac ggcccggcgg tgtaccttcc ggcagccccg ctggccgctc tcctgcctag
     2221 cgtcgccgcg ttctccaacc agaccgaggg attcctgacg agcgtatcgt ggggctaccg
     2281 cctggtgggc cgcctcgagt acaacaatgc gctcctcgga ggcaacctct ccccacggct
     2341 cgcctggtcg cacgacgtga acggcgtggg ccccaccttc aaccagggca tcaagtcgct
     2401 gtcgatcggc gcgagctggg aataccagcg caagtggctc gtggacgcgc agtacaccgg
     2461 ctacacgggc gggcgcacct attgcggcac cgacgttccg cccaccggca gcgtcgtcac
     2521 gcccggccag agcgcgaatt actgctcgtc ggccaacccc ctgaaggacc gcgacttctt
     2581 ctcggtcagc gtgagctact cgttctgacg gcgatccggg gcgcccagcg gcgccccgtt
     2641 gccgtccccc aataaaacaa catcggagga gattcaccat gttcgccatt acaaggaagg
     2701 ccacggcgct catcgccgtg ggcgccgcct tcgccattcc ggccgccgcg gagatgtccg
     2761 ccgccgacgt ggccaagctc ggcacgacgc tcacgccgat gggcgcggaa aaggccggca
     2821 acgccgcggg aacgatcccg gcgtgggacg gcggcatcac caagcccgtc gccggctaca
     2881 aggcgggcgg gcagtacccg gacccgttcc cggccgacaa gccgctcttc accatcgacg
     2941 gcaagaacgc cgaccagtac aaggaccagc tcgctcccgg ccagatggcg atgctgaaga
     3001 agtacccgac ctacaagatg atcgtgtacc cgtcgcgccg cagcgccgcc tttccgacgt
     3061 gccattacaa cgagacgaag gactgcgcgg ccaaggccag gctcgccccg ggcggaaacg
     3121 gcgtggtggg ttgcaccggc ggcattccgt tcccggttcc gaaggacggc aacgaggcga
     3181 tctggaatgc ggaactgcgc tatcgcggcg acacatttgc ccagcactgg gcgcaggccg
     3241 ccgtcacgcg caccggcgac tatgcgaccg tgaagttcga gtacgaatac gacttccagt
     3301 acggcaactg ctcgaagccg gtcgcgcagc gcgaaccgaa caagatcctc aactacctgc
     3361 aggtgaccac cgcgccggcg cgcctggcgg gtcagatcct gcttgttcac gagaccgtgg
     3421 accaggccaa ggagccgcgc tcggcctgga cctacaaccc cggccaacgt cgcgtgcgac
     3481 tggcgccgaa cgtcgcgtac gacaaccccg gcaccgcggc cgacggcctg cgcaccaacg
     3541 acgacttcgg catgtacaac ggggcgacgg accgctacaa ctggaagctc gtcggcaaga
     3601 aggagatgta catgccgtac aactccaacc gcatgaccgc gccgaacctc aagtacaccg
     3661 acgtcctcaa ggccgggcac ctcaaccagg atctcgcccg ctacgaattg caccgcgtct
     3721 gggtcgtcga ggcgaccctc aagccgggca cgagccacat ctacgccaag cgcgttttct
     3781 acatcgacga ggacagctgg gccatcctgg cgacggacaa gtacgacggt cgcggggaac
     3841 tgtggcgctt ctcggagcag cataacgaga actggtacaa cgtcccgatg ttcttcggca
     3901 cgatcgaggt gcacaacgac ctccagtcgg gccgctacat cgccatgggc ctgcgcaacg
     3961 aggagaacac gatctacaag ccgatcagcc gcagcggcgc cgactacgcg ccgaacagcc
     4021 tgcgcgctct cggcacgcgg tagtgcccga cccggccacc ggccgtccgg gggcagtttc
     4081 gcgcgcgtgg cgcgcgggga ctgccccctt ttcattgggg aacgggaaat gagcatgtcc
     4141 aaggtcgccg ggtcgctctt cgcggcgagc ctcgcgctcg ccacggtgcc gtcacgggca
     4201 gcggatatcc tgcccgagtc cgggcccatg ccaagggtca ccatggagcg cctgctcctc
     4261 gtcgatgcca cgcaccgcgg cagccgcgtg attgccgtcg gcgaccacgg ttacatcgtc
     4321 gtctcggacg acaacggcag gacctggcgc cgcgcgaagt cgccggccgc gccgctcctc
     4381 accgccgtcg atttcctcga cgcgaagagc ggctgggcgg tcgggcacga ttcggtcatc
     4441 ctcgcgaccg ccgacggagg cgaaacatgg gtccagcagt tcgcggcgcc cggcgagcag
     4501 cgcccgctcc tcgacgtgat cttcatcgac gggaacgccg gttacgcgat cggcgcctac
     4561 ggcgcgttct acgagaccac cgacgggggc aagagctgga acccgcgcaa ggtgatcggc
     4621 gacgacaagc acctcaatgc cttcgtgcgc cttccggggg ggcggctggt gatcctgggc
     4681 gaggccggca ccatcctcct ctccccggat gccggcaaga cgtgggaagc cgtcccgtcc
     4741 ccgtacaagg gctcgctctt cggcgggctg gaggccgatg acggatccgt catcgcgttc
     4801 gggctgcgcg ggcgcatctt ccgctcgacc gacgcgggca agacatggaa ggcggtcgac
     4861 aacccgtccg tcgccacgct catggggggc tcgaagcttc cggacggggc gctcgtgatc
     4921 gcgggggccg gcgggacagt gctcgtctcg cgcgacaatg gtctgtcgtt cgtgccgctt
     4981 gccaccggga gcaccaaggc ctactccaag gccgtgctcg ggtcggcgaa cgaggtgatc
     5041 ctgttcggcg agagcggcgt gcgcgaagtc gcgctgccgt cggccaagcg atagggcgaa
     5101 ccatgagcct cgacgcactg atccagtcgg tctcgcgctt cgtattcggc cagcgccgcg
     5161 ccctcctgat cgtcttcacg atcgcgacgg tgctgttcgc ggcttcggct tcgcgccttg
     5221 tcgtggatgc gggcttcaac aagatggtgc ctctcaagca cccgtacatg cgggtctacc
     5281 gcgactacga gaaggtgttc gggggcgcga accgcgtggc cgtggcgatc gtccagaagg
     5341 acggcgacat cttcaacaag gagtacatgg cgaagctgaa gggcctcacg gacgacgtat
     5401 tcctgctgaa cggcgtcgac cggccctcgg tcaagtcgct cttcacgccc aacacgcgct
     5461 tcatcgaggt gatcgaggag gggttctcgg gtggcaacgt gatcccggcc acgttccagg
     5521 gcaccgacga cgacctgaag caggtccgcg ccaacgtgca gaaatcgagc gagatcgggc
     5581 gcaccgtggc cacggacttc tccggggcgc tggtgacggc ggggctgctc gagatcgacc
     5641 cgcagaccaa ccagcgcctc aactacttcg aggtgtccgc gaggctcgag gccctgcgcg
     5701 cgaaatacga aggcccgaag cactcggtgc acatcatcgg cttcgccaag gcgatcggcg
     5761 acatccggga cggcgcgcgc ggcgtgctca cgttcttcgc cgtggccttc ctgatcaccg
     5821 cgggcctgat gctctggttc gtgaaggacg tgaagctcac gggggtggcc ctggtcgtgg
     5881 cgatgatgcc cgtcctgtgg ctcctcggga cgctgccgat cctcggctac gggatcgacc
     5941 cgctctcgat tctcgtgccc ttcctcatct tctccatcgg cgtttcgcac gcggtgcaga
     6001 tgtcgaagac ctgggagcgc gaggttgtcg ccgggaccga cagcctcacc gccgcaaagc
     6061 gcgccttcgc gaggctcttc attcccggga cgttcgcgct cctcaccaac gtgctcggct
     6121 tcgcggtgat catgatgatc cccatcgaca tcgtgcgcga gctcggcatc accgcctcgc
     6181 tgggcgtggc ctggatgatc atcaccaaca agatgctgct gcccatcctg ctttcgtacc
     6241 tgcagatggc caagacggcg gccaccaagg aggcgtacca ggaaagccgc gtcgagcgcg
     6301 tctggaaggc ggcctccggc tgcgtggaga aaggcagggc ggctgcgatc atcgcgatcg
     6361 ccctcgtcat ccttgtggcg ggcatcgtgg gggcgcacga cctgagagtg ggggactacg
     6421 gcataggcgt cccggaactg cgccccgact cgcgctacaa cagggacaac gcgaagatca
     6481 tcgagaagtt cgcgatgggc accaacacgc tgggcgtggt ggcccagacg aagggcgtgc
     6541 agggtgcgtg caccaactac gacctcatga gcgtcatcga gaggttcgac tggttcatgc
     6601 agaacgcgcc gggcacgcag tcggtcatct cgctgccggg cctcgcgaag aacgtcaatg
     6661 cggggttcaa cgaaggcaac gtcaagtggc gacagctgcc ccgcgacccg caggtgatgg
     6721 cccagagcgt cactcccatc gataccgcga cggggcttct caatccggac tgcagcgcga
     6781 tgcaggtgct cgtcttcacc acggaccagc agggcgccac catcgcatcg ctcgtgtcga
     6841 acgtgaaggc gtacgcggcg agcaacaact ccgacaagct ggagttcaag ctcgccacgg
     6901 gcaacatggg cgtgacggcg gcgacgaacg aggccgtgga ttcggcgcgc tgggagatga
     6961 acttcgcgat cttcggcgcg ctgatggtga tgtgcatgat caccttccgc agcctgcggg
     7021 ccaccctgtg catcctcctg ccgctggcgc tcgtctcggt gctgtgcgag gcgctcatgc
     7081 cgctgctcgg catcggactg aaggtctcga cgctccccgt gatcgcactg ggcgtcggcg
     7141 tcggcgtcga ctatggcatc tacctctacg atcgaatcga ggtgcacctg ggggagggca
     7201 agacgctcgc gacctcgttc ttcgaggcgc tgaaggagcg cggtacggcg atggtgttca
     7261 cggcggtgac gatgacgatc ggcgtgggta cctgggcgat gtcggccctc aagttccagg
     7321 cggacatggg cgtcctgctc gccttcatgt tcttcctgaa catgctgggc gcgatcttcc
     7381 tgctgccggc actggcggcg ttcctgatca agccgcacgg cgcggcgagg ggctgaggcg
     7441 cgcgcgtggc ggggctttcg cgcagcctca tcgcggccgg cgccgttgcg cttgccactg
     7501 gcgttgcgct gggcgccatc ggggcccatg cggcgaaatc cgccgcgcat cccgatgcgg
     7561 cacgactcat ccagaccgcc gtcttctacc agctggtgca cggcctgggg gcgttgatca
     7621 tcggggtcct ggcgcgaggc gacgggactt cgcgcctgct cgcgctttcc ggctggctgc
     7681 tgctcgcggg agtcgtcctc ttctgcggct cgctctatgc gctggcattc gccgcgctct
     7741 cgctcgggcc cgtggcaccg gttggcggca cggccttcat gaccggctgg gcgctcctcg
     7801 ccgtttgggc cgtgcggggc tagcaagagg tcatcctgac ctcgcatgca ggcgtaggtc
     7861 gtggggcatt tccgccttcg ccgcttcaaa acgcccggct cccggggcat atgccccgca
     7921 atccggccaa ggagatcgcg gcggtttcga ataatgcgcg cgaaaacaag cgattacaat
     7981 tgtagccgat cgctggcatg gcttgtgctg ttaagggatt acaggacctt ccctgaaggt
     8041 tccccaaacc cctaccaccg cacaaggaga atcacatgaa acgcaccatc gcccggatca
     8101 ccctcgccct cttcgcctcc agcctcggct ccgccttttt cgtcggcgac gcctttgcca
     8161 agaattccac cagcctgggc cacggcgtga aatgcacgtt cgtgctcgtc aagagcgtaa
     8221 acggcgtgaa cacctacaag accgtctgcc gcaggaatca ctaaaccctt gcggggttgg
     8281 ggagggggcc ggcgttccgg tctcctcccg gttctctttc tctagcctgc gcagggccgg
     8341 cgcgcaatcg ccggtctcga tggtgggtga tcgacaatcg ccgttgtccc attttgctgg
     8401 gggaagggga ggtcagatgc gcagtaacac ctcctgagtg gggtgttact gcgcaaatgc
     8461 ctcgagaacc gtgagtgcct ggccggggtc actggcgc
//
LOCUS       DASUEV010000314         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_778410, whole genome shotgun sequence.
ACCESSION   DASUEV010000314 DASUEV010000000
VERSION     DASUEV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778410"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFV55_09875"
     CDS             complement(<1..490)
                     /locus_tag="VFV55_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX4944649.1"
                     /translation="MPSPILPREEVKRLAARLVALGAIPIAVFCGAFALGRAALGATQ
                     QGQMWTWMLALGAAVVSVFVLARRTARVLDGEVQVIREAVRHTAGASAGTDLPELSPP
                     LEGLKREVQALATDFQAKEQRLQERLDNARQQVFFLASHDPLTGLANRKTLEERLESA
                     LAT"
     gene            complement(498..1202)
                     /gene="dnaQ"
                     /locus_tag="VFV55_09880"
     CDS             complement(498..1202)
                     /gene="dnaQ"
                     /locus_tag="VFV55_09880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX4944650.1"
                     /translation="MRRIVLDTETTGLEVVQGHRVIEIACVELFDRRPTGRNFHRYLN
                     PDREIDAGAIQVHGLTTDFLADKPHFPDIADEFIEFVEGAELLIHNAPFDVGFLNAEF
                     ALMGRPGIETICPVTDTLAMAREMHPGKRNSLDVLCERYQVDNSRRSLHGALLDAQLL
                     ADVWLAMTRGQDTLDISLMAEPAATAAGGPPARPARLEVLRASAAELAAHAAMCERID
                     RESRGRCLWKQAAPAG"
     gene            complement(1240..1731)
                     /gene="rnhA"
                     /locus_tag="VFV55_09885"
     CDS             complement(1240..1731)
                     /gene="rnhA"
                     /locus_tag="VFV55_09885"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX4944651.1"
                     /translation="MTAPHREAVTKAVEIYTDGACKGNPGPGGWGALLACDGKERELF
                     GGEASTTNNRMELTAVIEALAALKRPSRVILHTDSQYVHKGITEWIRGWKAKGWRTAS
                     KAPVKNVDLWKKLDILAAQHHVDWRWVKGHAGHDGNERADELANRGVVQGGEKPWDTD
                     EHG"
     gene            complement(1728..2486)
                     /locus_tag="VFV55_09890"
     CDS             complement(1728..2486)
                     /locus_tag="VFV55_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX4944652.1"
                     /translation="MTTNLLADWFGTPLGAYLLEKERAYLDDVTPDLFGFHALQLGLP
                     QFDLLRENRITHRMRIDAGEGATVRARSHELPIATQSIDLALLPHVLEFAENPHEVLR
                     EIDRVMMPEGRIVILGFNPWSLWGLRSVLGPSRHETPWNGKFVSLLRAKDWLALLGYE
                     VSAGRLICYVPPVRKEKLLRRLAFMEPAGDRWWAVGGAVYMLQAIKRVRGMRLLTPAW
                     HEALAREKRVALAAKREGAGAAATSRAGSLRIVK"
     gene            2513..3307
                     /gene="gloB"
                     /locus_tag="VFV55_09895"
     CDS             2513..3307
                     /gene="gloB"
                     /locus_tag="VFV55_09895"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX4944653.1"
                     /translation="MMQIHAVPAFDDNYLWVIEDGRNAAVVDPGDEAPVQTFLEAKGL
                     TLTAILVTHHHGDHVGGVEALAAHWRCAVFGPAAERIAGLTTRLREGDHLTVPGLGLP
                     LEVLEVPGHTAGHIAYLGTGAPTESGIGPVVFCGDTLFACGCGRLFEGTPAQMVDSLA
                     KLARLPGGTRMYCAHEYTMSNIRFAQVVEPGNAKLAVRKARDAAARAENRPTLPSTIA
                     DELDTNPFLRCTSAEVVASASKHAGRPLATPLEVFTAVREWKNNFR"
     gene            3430..3792
                     /locus_tag="VFV55_09900"
     CDS             3430..3792
                     /locus_tag="VFV55_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4944654.1"
                     /translation="MHEALFGKRPACEAVMAERGGRAVGFALFFTTFSTFLCKPGLYL
                     EDLFVEPGHRGAGIGKAMLRHLAALAVERDCGRFEWRVLDWNEPSIRFYESIGGKLLP
                     EWQLVRMTQPEFSALARR"
BASE COUNT          584 a         1421 c         1306 g          666 t
ORIGIN      
        1 ccgtggcgag cgccgattcg agccgctcct ccagcgtctt ccggttggcc agcccggtga
       61 gcgggtcgtg gctggcgagg aagaacacct gctggcgcgc gttgtccagg cgttcctgca
      121 ggcgctgctc cttcgcctgg aagtcggtgg cgagggcctg cacttcgcgc ttcaggcctt
      181 cgagcggggg gctgagttcg ggaagatccg tgcctgccga agcgccggcc gtgtgccgga
      241 ccgcctcgcg gatgacctgg acttcgccat cgaggacgcg cgcggtgcgt ctcgccagca
      301 cgaataccga caccacggcg gccccgagcg ccagcatcca tgtccacatc tggccctgct
      361 gggtggcgcc cagcgccgcg cgcccgaggg cgaatgcgcc gcagaagacg gcgatgggaa
      421 tggcccccaa agccaccagc cgcgccgcca accgctttac ctcttccctg gggaggatcg
      481 gagagggcat cgggctccta accggccggt gccgcctgct tccacaggca gcgacccctg
      541 ctttccctgt cgatgcgctc gcacatcgcg gcgtgcgccg ccagttccgc ggcgcttgcg
      601 cgaagcacct cgaggcgggc cggacgagcg ggcgggccgc ctgccgcggt tgccgcaggt
      661 tcggccatca gggagatgtc gagcgtgtcc tgcccgcgcg tcattgcgag ccagacgtcg
      721 gccagcagct gcgcatccag cagcgcgcca tgcagcgagc gccgcgagtt gtccacctgg
      781 tagcgctcgc acaatacgtc gagggaattg cgcttgccgg gatgcatctc gcgcgccatg
      841 gcgagggtgt cggtcaccgg gcagatcgtc tcgatgccgg gccggcccat gagcgcgaac
      901 tccgcgttca ggaagcccac gtcgaagggc gcgttgtgga tgagcagctc cgcgccctcg
      961 acgaactcga tgaactcgtc ggcgatgtcg gggaaatgcg gcttgtcggc gaggaagtcg
     1021 gtggtgagcc cgtgcacctg gatcgcgccc gcatcgatct cgcggtcggg gttgaggtag
     1081 cggtggaagt tgcgccccgt cggccgccgg tcgaagagct ccacgcacgc gatctcgatc
     1141 acgcggtggc cctggaccac ctcgagcccc gtcgtctccg tgtccaggac gatccgcctc
     1201 accgcccgga ccctttgctc atccgagtcc tatccgtgtt catccgtgtt catccgtgtc
     1261 ccaaggcttt tccccgcctt ggacgacacc gcgattggcc agctcatccg cccgctcgtt
     1321 cccgtcgtgc cccgcatgcc ccttcaccca ccgccagtcg acgtgatgct gcgccgccag
     1381 gatgtcgagc ttcttccaca ggtccacgtt cttcaccggc gccttcgagg ccgtccgcca
     1441 ccctttcgcc ttccacccgc ggatccattc cgtgatcccc ttgtgcacgt actgcgaatc
     1501 ggtgtgcagg atcacccggc tcggccgctt cagggcggcc agcgcctcga tcacggcggt
     1561 gagttccatc cggttgttgg tggtcgacgc ttccccgccg aagagctcgc gctccttgcc
     1621 gtcacaggcg agcagcgctc cccagccccc ggggccggga ttgcccttgc atgcgccgtc
     1681 ggtatagatc tcgaccgctt tggtaacggc ttctcggtgg ggggcggtca cttgacgatg
     1741 cgcagggacc ctgcccggga agttgccgcc gcgcccgcgc cctcgcgctt ggccgccagc
     1801 gccacgcgct tctcgcgcgc caacgcctcg tgccatgccg gcgtgagaag gcgcatgccg
     1861 cgcacgcgct tgatggcctg cagcatgtac acggccccgc ccacggccca ccagcggtcg
     1921 ccggcgggct ccatgaaggc aaggcgcctc agcaatttct ccttcctcac cggcgggacg
     1981 tagcagatga gccgccccgc cgagacctcg tagcccagca gcgccagcca gtccttcgcg
     2041 cgcagcagcg aaacgaactt gccgttccat ggcgtttcgt ggcgcgacgg tcccaggacg
     2101 cttctcaggc cccacaggct ccacgggttg aagccgagga tgacgatgcg gccctcgggc
     2161 atcatcacgc ggtcgatctc gcgcagcacc tcgtgcggat tctcggcgaa ttcgagcacg
     2221 tgcggcagga gggccaggtc gatcgactgg gtggcgatcg gcaactcgtg actccgggcg
     2281 cgaacggtcg cgccctcgcc cgcgtcgatc ctcatgcggt gcgtgatgcg gttctcgcgc
     2341 agcaggtcga actggggcag gcccagttgc agcgcgtgga acccgaagag gtccggcgtg
     2401 acgtcgtcca ggtaggcgcg ctccttttcc aggagatagg ccccgagcgg cgtgccgaac
     2461 cagtcggcga gcaggttggt cgtcatgggt caattctaat agactcgggt ccatgatgca
     2521 gatccatgcg gttccggcgt tcgacgacaa ctacctctgg gtcatcgagg atggccgcaa
     2581 cgccgccgtc gtcgatccgg gcgacgaggc gcccgtgcag acgttcctcg aggcgaaggg
     2641 gctcacgctc accgccatcc tggtcaccca ccaccacggc gaccacgtgg gcggcgtgga
     2701 agcgctggcc gcgcactggc gatgcgcggt gttcgggccc gccgcggagc gcatcgccgg
     2761 gctgacgacg aggcttcgcg agggcgacca cctgaccgtg ccgggtctgg gtctcccgct
     2821 cgaagtgctc gaggtgccgg gccataccgc ggggcacatc gcctacctgg gaaccggtgc
     2881 cccgacagag tcgggcatcg gcccggtggt cttttgcggt gacacgcttt tcgcctgcgg
     2941 ctgcggccgc ctcttcgagg ggaccccggc gcagatggtc gattccctcg ccaaactcgc
     3001 gaggctgccc ggcggcacgc gcatgtactg cgcccacgaa tacacgatgt cgaacatccg
     3061 tttcgcgcaa gtcgtggagc cgggcaacgc gaagctcgcg gtccgcaagg ctcgcgatgc
     3121 ggcggcgcgg gcagagaatc gcccgacact cccctcgacg atcgccgacg agctcgacac
     3181 caaccccttc cttcgctgca catcggcaga ggtcgtcgcc tcggcctcga agcacgccgg
     3241 ccgccccctc gccacgcccc tcgaggtctt caccgcggtg cgcgaatgga agaacaactt
     3301 ccgctgacca tggccgactt caccctgcga tccgccgagc cgcgcgactg cggcgacctc
     3361 aaccgcctca tcctcgccct ggctgaatac taaaagctcg cccaccaggc cgcgcccacg
     3421 cccgaagggc tgcacgaagc cctcttcggc aagcgcccgg cgtgcgaagc cgtgatggcc
     3481 gagcgcgggg gacgcgccgt gggcttcgcc ctcttcttca cgacgttttc cacgttcctg
     3541 tgcaagcccg gcctctacct ggaggacctc ttcgtggagc cggggcaccg cggcgcaggc
     3601 atcggcaagg cgatgctgcg ccacctcgcc gccctcgccg tcgagcgcga ctgcggccgc
     3661 ttcgagtggc gcgtcctcga ctggaacgaa ccgtcgatcc ggttctacga gtcgatcggc
     3721 ggcaagctcc tccccgagtg gcagctcgtg cgcatgacgc agccggagtt ctccgcactg
     3781 gcacggcgct gatcgcccca acgggcacgg ctgcatcact ggaggcaatg cccggccgct
     3841 gtatcgcgaa cgttcttcct gggcacggcg gtgaaagtat ggaccggttc ggggtccgat
     3901 tggctgcttc gaatttttct ttccggccag gtcaagttgc gtcgggcgtc gcggatcgaa
     3961 cctatgtcga ggtttcc
//
LOCUS       DASUEV010000315        10583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1234411, whole genome shotgun sequence.
ACCESSION   DASUEV010000315 DASUEV010000000
VERSION     DASUEV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234411"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV55_09905"
     CDS             complement(<1..336)
                     /locus_tag="VFV55_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX4944655.1"
                     /translation="MPQEFSRIKRLPPYVFNITNELKMAARRAGEDVIDLSMGNPDGP
                     TPRHIVDKLVEVAQREDTHGYSVSKGIPRLRRAICQWYRRRWNVDFDPESEAIVTIGS
                     KEGLAHLMLA"
     gene            553..861
                     /locus_tag="VFV55_09910"
     CDS             553..861
                     /locus_tag="VFV55_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944656.1"
                     /translation="MTARTPHLDPEARKQRLLREALGNAGGFMPSAERVGKVFVAVGL
                     SLTAAVAISSGLPFRSERVVTLAAYPNVPSAGQGLPVTQPGGEDDRAAPEGNVQDLTY
                     "
     gene            976..1653
                     /locus_tag="VFV55_09915"
     CDS             976..1653
                     /locus_tag="VFV55_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944657.1"
                     /translation="MLLGKLLTSLVVVIPLSTLAQLPGEHVRYQEAPHHYPYVSINGP
                     LLPIWKMTLDDPRDNIVYGLATMVTRSNGEGATVGGQINAWAGNGITGQTWGTATEAV
                     GMKGNKSILVGLEALVANQEPEHDLPKIGINIVFNNGGDLDSAGEGSNVNSVGLWFTA
                     TSMKSFESAIKLDRRSISGSISKPRPAVLDLEDLDPDTIGEVDLIRLPGGKSIRFNPY
                     TSQLEAR"
     gene            1947..2432
                     /locus_tag="VFV55_09920"
     CDS             1947..2432
                     /locus_tag="VFV55_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX4944658.1"
                     /translation="MQKSLQIDPAKCTGCLQCEMACSYENYQVFNPAKSRIKVFDFHH
                     TGRKVPYTCTQCDEAWCLHACPVEAIKVDRDTGAKVVLEDVCVGCKVCTIACPFGTVN
                     YVQDTGKVQKCDLCFGKPACAEACPTGAITYVDADWTGLDRMRQWADKLDNQPAAGNA
                     D"
     gene            2445..4304
                     /locus_tag="VFV55_09925"
     CDS             2445..4304
                     /locus_tag="VFV55_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX4944659.1"
                     /translation="MSWTRKILRVDLTKGTCTPEPLNMDWARSYLGQRGLATKYFTAE
                     VDPKVDPLSAANKIIFATGPLTGTMASTGGRYSVITKGPLTGAIACSNSGGYFGAELK
                     FAGWDMIIVEGKSAKPVLLHIEDDKAELLDASDVWGKSTWDTEGLLKKRFQDPQVRVS
                     SIGPAGETGCLYAGVINDLHRAAGRSGVGTVMGSKNLKAIAVRGTKGVGNIKDPVAFM
                     KATNAAKKVLADNAVTGQGLPKFGTQVLMNVINEIGALPTRNHKDVQFEGAKDISAEA
                     MHAPRRTDGKANLVTNQACFGCTIACGRISKIDETHFTVVNKPQYWGASGGLEYEAAW
                     ALGAANGVNDLDALTYANFIANEDGWDPISFGATVGAVMELYEMGVLKKEQLGIEAPF
                     GSAKALTFFAEQVGKGTGFGKEIAMGSKRLTAKYGHPDLSMTVKGQEFPAYDSRGIQG
                     MGLAYATANRGACHLRGYTVASEVLGIPIKTDPLTTEGKPGLVKAFQDATAVFDSSGL
                     CLFTSFAWTLQDVGPQVQAACEGDWSIENLNLVGERIWNLEKEFNLAAGLTKKDDDLP
                     KRLKTEPAKTGPAKGLVNGIEKMLPEYYELRGWSKEGVPTAETRKRLDTAALG"
     gene            4316..5569
                     /locus_tag="VFV55_09930"
     CDS             4316..5569
                     /locus_tag="VFV55_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX4944660.1"
                     /translation="MRHLILGNGPAGVIAAETLRKHAPADAIALVGDEPGPPYSRMAI
                     PYLLMGHIEESGTWLRKDPAHFDRLGITLKTGKARSVDTKARAVALESGESLAYDRLL
                     IATGATPTRPPIPGIELSGVVPCWTLADAHRIMALAKPGSRVVQMGAGFIGCIIMEAL
                     AERGVKLTMVEMGDRMVPRMMTEGASALIKAWCEKKGIRVITGARVKSITAAGSTLTA
                     NLESGQALEADLVISATGVKPNTGFLQGSGIACGQGILVDDHLRTNVPEVYAAGDVAE
                     AAEFATGRTVVYAIQPVAAEQARIAALNMVGKEATSPGTFAMNVLDTLGLVAASFGQW
                     GGVPESEGGASVEVRDDDAFRYLRLEFADDCLIGATSLGLTEHVGVIRGLIQGRVPLG
                     EWKKKLVADPMRLMDAYLARAQAAS"
     gene            5577..5831
                     /locus_tag="VFV55_09935"
     CDS             5577..5831
                     /locus_tag="VFV55_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX4944661.1"
                     /translation="MRVTLKLYATLTDYLPAAARRDNFVLMDVDESATIASLVEPLGL
                     PPRLVHLVLVNGVYIPPAERAARAFRDGDVLAIWPPIAGG"
     gene            5845..6126
                     /locus_tag="VFV55_09940"
     CDS             5845..6126
                     /locus_tag="VFV55_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944662.1"
                     /translation="MQYRYEMSLTRADFLRLLPVAVGHAAWRMEGDDIAGIGVQPAWR
                     IRLEERPGRPFGPVELPVLAVTLDLDGASGDHCAAFVERFLLGFQRAGG"
     gene            6170..6541
                     /locus_tag="VFV55_09945"
     CDS             6170..6541
                     /locus_tag="VFV55_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX4944663.1"
                     /translation="MKISLEAAEGRNLFTAYGEGYVDVNGTRHRANVVVSADAVEPAW
                     SEGPLEALTEAHMDRLAAARPEIVLLGSGATFRFPPPALLAPLHRAGIGVEVMDTRAA
                     CRTYNILLGEGRRVVAALIVE"
     gene            complement(6572..7765)
                     /locus_tag="VFV55_09950"
     CDS             complement(6572..7765)
                     /locus_tag="VFV55_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4944664.1"
                     /translation="MPDTDEMPPGRWGLVAALGVTQIVSWGSIYYAFAVVMESVQREL
                     GTTAPATVGAYSVALLVSGLVAAPVGRHIDRHGARRAMTAGSVAAALLLAAFSQVTNA
                     TALYAVWAALGVAMGLTLYEPAFASLALVFRADLRKAITVLTLAGGFASTVFWPLTQW
                     LASSLGWRDAMLVLAAINLFVCVPLHAIYLPARGRPTDAPPGAADDAAGRARLLADRR
                     FRWLAVAFTLNMLAFAAVGLHLLAMLKEQGFAPEKAALLAALVGPMQVAGRIAEFAFA
                     PRVSPAKVGEIALFVFPIALAILAFAGGSAWGVIAFAVLYGASNGVMTIVRGTVPAEI
                     WGREGYGGLTGLMATPVLLARAAGPFAAAGILALAGGYRAVSLSLIAVGIVSWAAFAM
                     AVRRR"
     gene            complement(7758..>10583)
                     /locus_tag="VFV55_09955"
     CDS             complement(7758..>10583)
                     /locus_tag="VFV55_09955"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX4944665.1"
                     /translation="VLRAYARYLRQAGFTFSQAYIAQTLAAHPAIVAMLVAIFQARFD
                     PAAGADREAAQQRLHDEAKAALNGVANLDEDRILRRFLYAIRATIRTNHWVRDAGGRR
                     KPYLSLKIDCAMVPDLPEPRPLFEIFVYSPRFEAIHLRGGKVARGGIRWSDRPEDFRT
                     EVLGLMKAQMVKNAVIVPVGSKGGFVLKAAPPASDREAYLKEGVACYQDFLRGLLDVT
                     DNLQGGKIVPPRDVVRHDPDDPYLVVAADKGTAAFSDYANAVSAEYGHWLGDAFASGG
                     SAGYDHKKMGITARGAWESVKRHFREAGLDTQSQDFTVVGVGDMSGDVFGNGMLLSRH
                     IKLVAAFDHRHIFLDPDPDTAASFRERERLFALPRSSWEDYDRSVLSRGGGVYPRTAK
                     SIALSAEARTALGIVEEALTPQELMKAILKARVDLLYNGGIGTYVKASRQTHADAGDR
                     ANDAIRVNAAELRCKVVCEGGNLGFTQLGRIEFALAGGRICTDAIDNSAGVDCSDHEV
                     NIKILLGLVVADGELTEKQRNKLLAAMTDEVGRLVLADNYFQTQSLAVSGMRGEKLLD
                     AQASLIRALEKAGRLNRAVEYLPSEDEIAERRALRAGLTSPERAVLLAYSKMVLYDDL
                     LKGTLVDDEFVARALVAYFPAELTKRYAAVMPRHPLRREIIATVVANSMINRTGSVFV
                     HRMQEETGATPDEVTRAFILVRDAFGFEALWSAIDGLDNRVSADLQSQMLIEAGRLVL
                     RATLWFLRRRREKLPIAQVLAIFQPGLAAFRRQLPAILSAADRENFDAAVASLVAKGV
                     PQDLARAMAELDALYAVLDVTEVAQETGRPLEAVARVYFALAGDLELHWFAERITALP
                     TETSWQALARNALRDDLSSQQRLLATSVTRLSPGSTDPAAMVAAWKERYAQPIARLAA
                     MRGELKRAASLDLAVLSVLLRELRGLA"
BASE COUNT         1826 a         3619 c         3555 g         1583 t
ORIGIN      
        1 cgcgagcatc aggtgcgcga ggccctcctt cgagccgatg gtgacgatgg cctcgctttc
       61 cggatcgaaa tccacgttcc agcggcgcct gtaccactgg cagatggcgc ggcgaaggcg
      121 cgggatgccc ttcgagaccg agtagccgtg cgtgtcctcg cgttgcgcca cctcgaccag
      181 cttgtccacg atgtgccgcg gcgtgggccc gtcggggttg cccatggaca ggtcgatgac
      241 gtcctccccc gcccggcggg cggccatctt gagctcgttg gtgatgttga aaacgtaggg
      301 cgggagtcgc ttgatgcggg aaaattcctg cggcatgggg atgtccgggg agcggtcgtt
      361 cgcgcgcgag ccgggttgct gcgctgcaaa ataatcattc tagcgggcgg ggcgcgttcg
      421 cggcaaaccc ggtcgcggcg cacgccatct ttcagcaacg tttaaggact ccttaaggac
      481 gcaggcacca ccaggggcgg acagtcctcc catcgacaac gcagccctgc tgcattcccg
      541 aaggagccca cgatgaccgc ccgcaccccc cacctcgacc cggaagcccg caagcaacgt
      601 ctcctgcgcg aagccctcgg aaatgcgggc ggcttcatgc cctccgccga acgcgtcggc
      661 aaagtcttcg tcgcggtcgg actctccctt acggcagcag tcgcgatatc cagcggcctg
      721 cccttcagaa gcgaaagggt ggtgacgctg gcagcctacc cgaacgtccc ctcggcagga
      781 cagggcctgc cggtcaccca acccggcggc gaggacgacc gcgcggcgcc cgaaggcaac
      841 gtgcaggacc tgacgtacta gcgcaggaac gcagcggatg atttttcatg gcttgcacca
      901 aagagtgcaa cccgttcgag ggaagggccg ggaagcggat acctgggaag caggattccg
      961 taaagggata cgtccatgct gctcgggaaa cttctcacgt cgctcgtcgt tgtcattccc
     1021 ctttcgacgc tcgcgcagct tccaggggag cacgttcgat accaggaagc tccgcatcac
     1081 tacccgtacg tctcgatcaa tggcccgcta cttcccatct ggaagatgac cctggatgat
     1141 ccgagggaca acatcgtcta cggtctcgcc acgatggtca cgcgctccaa cggcgaaggt
     1201 gccaccgtcg gcggccagat caacgcatgg gctggcaacg gcatcaccgg acagacgtgg
     1261 ggtaccgcta ccgaggccgt gggcatgaag ggtaacaagt caatcctggt cgggctggaa
     1321 gcattggtcg ccaaccagga accagaacat gacttgccga agatcggcat caacatcgtg
     1381 ttcaacaacg gaggagatct tgattcggcg ggcgaaggtt ccaacgtcaa cagcgtcggg
     1441 ctgtggttta ccgcgacttc gatgaagagc ttcgagtcgg cgatcaagct ggaccgccgg
     1501 tcgatctcgg gatcgatcag caagccacgg cctgcggtac ttgaccttga ggatctcgac
     1561 ccggacacga tcggggaagt tgacctgatt cgccttccgg gaggcaaatc gatccggttc
     1621 aacccctaca ccagccagct cgaggcgcgc tagccgggtt tgattccgca ctcagccgtg
     1681 attgcagcga ttcggggggc tggcgttcgc gcgcgagccg ggttgctgcg ccgcaacaga
     1741 gcgatccccg gggccgcgtc gcccggcgga acaccgggcc ggagccgcga ataggacgcg
     1801 attgccatat ggcgtcgaat gcctataatg ggctcgcact ttcggggccc cgtttcgccc
     1861 cgtcacttcc ggcttacggc gcaaatggcc cgtctcgagg cctcccggcg ccgcccgatg
     1921 accgctttcc cgaggaggag aggatcgtgc agaaatcctt acagatcgat ccggcgaaat
     1981 gcaccggttg cctgcagtgc gagatggctt gctcgtacga gaactaccag gtattcaatc
     2041 ccgccaagtc gcgcatcaag gtcttcgatt tccaccacac cggccgcaag gtgccctaca
     2101 cctgcacgca gtgcgacgag gcctggtgcc tgcacgcctg cccggtggaa gccatcaagg
     2161 tggaccggga caccggcgcg aaagtcgtgc tcgaggacgt ctgcgttggc tgcaaggtgt
     2221 gcaccatcgc ctgccccttc ggcaccgtga actacgtgca ggacaccggc aaggtgcaga
     2281 aatgcgacct gtgcttcggc aagcccgcct gcgcggaggc ctgcccgacg ggcgccatca
     2341 cctatgtgga cgccgactgg accggtctgg accggatgcg ccagtgggcc gacaagctcg
     2401 acaaccagcc ggccgccggc aacgcggact gataggaggc caccatgtcc tggacacgca
     2461 agattctccg agtcgatctc acgaagggca cctgcacgcc ggaaccgctc aacatggact
     2521 gggcgcgcag ctacctcggg cagcgcggcc tcgccaccaa gtacttcacc gcggaggtcg
     2581 atcccaaggt cgatcccctc tcggccgcga acaagatcat cttcgccacg ggcccgctca
     2641 cgggcaccat ggcctccacc ggcgggcgct attccgtcat caccaagggc ccgctcaccg
     2701 gcgccatcgc ctgctccaac tccggtggct acttcggcgc ggagctgaag ttcgccggct
     2761 gggacatgat catcgtcgag ggaaagtcgg cgaagccggt tctcctgcac atcgaggacg
     2821 acaaggcgga gctcctcgac gcttccgacg tctggggcaa gtccacctgg gacaccgagg
     2881 gcctgctcaa gaagcgcttc caggacccgc aggtgcgcgt ctcgagcatc ggcccggccg
     2941 gcgagacggg ctgcctgtac gccggcgtca taaacgacct gcaccgcgcg gcgggccgct
     3001 ccggcgtggg caccgtgatg ggatcgaaga acctcaaggc gatcgcggtc cgcggcacga
     3061 agggcgtggg caacatcaag gacccggtgg cgttcatgaa ggccaccaac gcggcgaaga
     3121 aggtcctcgc ggacaacgcg gtcacgggcc aggggctccc caagttcggc acgcaggtgc
     3181 tgatgaacgt gatcaacgag atcggggcgc tgcccacgcg caaccacaag gacgttcagt
     3241 tcgagggcgc caaggacatt tccgccgagg cgatgcacgc cccgcgcagg accgacggca
     3301 aggccaacct cgtcaccaac caggcgtgct tcggctgcac catcgcctgc gggcgcatct
     3361 cgaagatcga cgagacgcat ttcacggtgg tcaacaagcc ccagtactgg ggcgcctcgg
     3421 gcggcctcga gtacgaggcg gcgtgggcgc tgggcgccgc caatggcgtg aacgacctcg
     3481 atgccctcac ctacgcgaac ttcatcgcca acgaggatgg atgggacccg atctccttcg
     3541 gcgcgacggt gggggcggtg atggaactct acgaaatggg cgtgctcaag aaggagcagc
     3601 tcggcatcga ggcgcccttc ggttccgcca aggccctcac cttcttcgcc gagcaggtcg
     3661 gcaagggaac gggcttcggc aaggaaatcg cgatggggtc gaaacgcctc acggccaagt
     3721 acggccaccc ggacctgtcc atgaccgtga aaggccagga gtttcctgcc tacgactcgc
     3781 gcggcatcca gggcatgggc ctcgcctacg ccacggccaa ccgcggcgcc tgtcatcttc
     3841 ggggttacac ggtcgcgagc gaagtattgg gcataccgat caagaccgac ccgctcacga
     3901 ccgaaggcaa gcccggcctg gtgaaggcgt tccaggacgc caccgcggtg ttcgattcgt
     3961 cggggctttg ccttttcact tccttcgcgt ggacgctgca ggacgtcggg ccgcaggtgc
     4021 aggccgcctg cgagggcgac tggtcgatcg agaacctcaa cctcgtgggc gagcgcatct
     4081 ggaacctgga gaaggaattc aacctggccg ccgggctcac gaagaaggat gacgacctgc
     4141 ccaagcgcct gaagaccgag ccggccaaga ccgggccggc caagggcctg gtgaacggga
     4201 tcgagaagat gctgcccgag tactacgagc tgcgcggctg gagcaaggaa ggcgtgccga
     4261 cagccgagac gcgcaagcgc ctcgatacgg ctgcgctcgg gtagccgctg cggccatgcg
     4321 ccacctgatc ctcggcaacg gcccggcggg cgtcatcgcc gccgagacgc tgcgcaagca
     4381 cgcgcccgcg gacgccatcg cgctcgtggg cgacgaaccc ggcccgccct actcccggat
     4441 ggcgatcccc tacctgctga tgggacacat cgaggagtcg ggcacctggt tgcgcaagga
     4501 tccggcgcat ttcgaccggc tgggcatcac gctcaagacc ggcaaggcgc gctcggtcga
     4561 tacaaaggcg cgtgcggttg cgctcgaatc gggcgagtcg ctcgcctacg accggctcct
     4621 catcgccacg ggcgcaacgc caactcgtcc gcccatcccg gggatcgaac tgtccggcgt
     4681 ggtcccgtgc tggaccctgg cggacgcgca ccggatcatg gcgctcgcca agccgggaag
     4741 ccgcgtcgtg cagatgggcg cggggttcat cggctgcatc atcatggagg cgctcgccga
     4801 gcgcggcgtg aagctcacca tggtcgagat gggcgaccgg atggtgccgc gcatgatgac
     4861 cgaaggcgcc tccgccctta tcaaggcatg gtgcgaaaag aagggaatcc gcgtcatcac
     4921 gggcgctcgc gtgaagtcga ttaccgccgc gggcagcacg ctcaccgcga acctcgagag
     4981 cggacaggcg ctcgaggccg acctcgtcat ctcggccacc ggcgtgaagc ccaataccgg
     5041 attcctccag ggcagcggca tcgcctgcgg gcagggcatc ctcgtggacg accacctgcg
     5101 gaccaacgtg cccgaggtgt atgccgccgg ggacgtggcc gaagccgcgg agttcgcgac
     5161 gggccgcacg gtggtgtacg cgatccagcc ggtggccgcc gagcaggcgc gcatcgcagc
     5221 cctcaacatg gtcggcaagg aagccacatc gccgggcacc ttcgccatga acgtgctcga
     5281 cacattggga ctggtggccg cctcgttcgg gcagtgggga ggagttcccg agtccgaggg
     5341 aggcgcatcc gtggaagtca gggacgacga tgccttccgt tacctgcgcc tcgagttcgc
     5401 cgacgactgc ctcatcgggg ccacgagtct cgggcttacg gagcatgtgg gcgtgatccg
     5461 cggcctcatc cagggccgcg tccccttggg cgagtggaag aagaaactcg tcgcggatcc
     5521 catgcgcctg atggacgcct acctcgcgcg cgcgcaggcc gcgtcctgaa ggcacgatgc
     5581 gcgtcaccct caagctctac gcgacgctca ccgactacct gcccgccgcc gcgcggcgcg
     5641 acaacttcgt gctcatggac gtcgacgagt cggcgacgat cgcctcgctc gtggagccgc
     5701 tgggcctgcc gccgcggctc gtccacctcg tcctcgtgaa cggcgtgtac attccgccgg
     5761 ccgaacgggc cgcccgcgca ttccgcgacg gcgacgtgct cgcgatctgg ccgcccatcg
     5821 ccggggggtg acgcgcttcc cttcatgcag tatcgctacg agatgtccct cacgcgagcc
     5881 gatttccttc gcctgctgcc cgtggcggtc ggccacgccg cctggcgcat ggagggcgac
     5941 gatatcgcgg gcattggggt acagccggcg tggcgcatcc ggctggagga aagacccggc
     6001 aggccattcg ggcccgtcga attgccggtg ctcgccgtca cgctcgacct cgacggcgcc
     6061 tccggggacc attgcgcggc tttcgtggag cgattccttc tcggcttcca gcgcgcgggc
     6121 gggtgaggcg tcgcgccgcg gctgcgggcc gatgcgacaa tgacgggtca tgaagatatc
     6181 gctcgaggcg gccgagggcc gcaacctttt caccgcgtac ggtgagggct acgtggacgt
     6241 gaacggcacg cgccatcgcg ccaacgtcgt ggtgagcgcc gacgccgtcg agcccgcgtg
     6301 gtccgagggc ccgctcgagg ccctcacgga agcgcacatg gaccgccttg ccgccgcgcg
     6361 gccggagatc gtgctgctgg gttccggggc gaccttccgc ttcccgcccc ccgcgctcct
     6421 cgcgccgctc caccgggctg gcatcggcgt tgaagtcatg gatacccggg ccgcgtgccg
     6481 cacctacaac atcctcttgg gcgaaggccg gcgcgtcgtg gccgctctca ttgtcgagta
     6541 gcgcggtgtc agacaccatc accgggcacc gtcaacgcct gcgtacggcc atcgcgaatg
     6601 cggcccacga cacgatgccc accgcgatca gggaaagcga cacggcacgg tagccgccgg
     6661 ccagcgcgag gatgcccgcc gccgcgaacg gccccgccgc cctggcgagc agcacgggag
     6721 tggccatgag gccggtcaac cccccatagc cttcgcgccc ccagatttcg gccggcacgg
     6781 tgccgcgcac gatcgtcatc accccgttgc tcgccccata gagaacggcg aacgcgatca
     6841 ccccccaggc gctcccgccg gcaaaggcga ggatcgcgag ggcgatggga aacacgaaga
     6901 gcgcgatctc gcccaccttc gccgggctca cccgcggcgc aaacgcgaat tccgcgatcc
     6961 gccccgccac ctgcatcggt cccaccaacg ccgcgaggag cgccgccttc tccggtgcga
     7021 agccctgctc cttgagcatc gcgaggaggt gcaggcccac ggcggcgaac gccagcatgt
     7081 tgagcgtaaa ggcgacggca agccagcgga aacggcgatc ggcgagcagc ctcgcgcgcc
     7141 ccgccgcatc gtcggccgct cccgggggtg cgtcggtcgg acggccccgc gcgggaaggt
     7201 aaatggcgtg cagcggcacg cagacgaaaa ggttgatggc cgcgagcacc agcatcgcgt
     7261 cgcgccatcc cagcgagctc gcgagccact gggtgagcgg ccaaaagacg gtgctcgcga
     7321 atccgccggc cagcgtgagc acggtgatcg ccttgcgcag gtcggcgcga aagaccaggg
     7381 cgaggctggc gaaggccggc tcgtagagcg tgagccccat ggccacgccc agcgccgccc
     7441 acacggcgta gagcgcagtc gcgttcgtga cctgggagaa cgccgccagc agcagcgcgg
     7501 cggcgacgga accggccgtc atcgcccgcc gcgcgccatg gcggtcgatg tgccgcccca
     7561 cgggagccgc gacgagtccc gacacgagaa gcgcgaccga gtacgccccg acggtcgccg
     7621 gcgccgtggt gccgagctcg cgttgcacgg attccatcac caccgcgaag gcgtagtaga
     7681 tgctgcccca ggagacgatc tgggtgacac cgagggcggc gacgagaccc cagcgccccg
     7741 gcggcatttc gtccgtgtca ggcaagtccg cgcagttccc tcaggaggac ggagagcacg
     7801 gcgaggtcga gcgacgcggc gcgcttgagt tccccgcgca tggccgccag ccgcgcgatg
     7861 ggctgcgcat agcgctcctt ccacgcggct accatcgccg ccggatccgt cgagcccggc
     7921 gagagcctgg tgacggaagt cgcgagcagg cgctgctggc tcgacaggtc gtcgcgcagc
     7981 gcgttgcgcg ccagcgcctg ccaggacgtt tccgtgggca gcgccgtgat gcgctcggca
     8041 aaccagtgca gttccaggtc gccggccagc gcaaagtaga cccgcgctac ggcctccagc
     8101 ggccgtcccg tctcctgcgc tacctcggtc acgtcgagca cggcgtaaag cgcatcgagc
     8161 tcggccattg cgcgcgccag gtcctgcggc acacccttgg cgacgaggct cgcgaccgcg
     8221 gcatcgaagt tctcgcggtc ggccgcggaa aggatcgcgg gcaactgccg gcggaaggcg
     8281 gccaggcccg gctggaagat cgcgaggacc tgggcgatcg ggagcttctc gcgccggcgg
     8341 cgcaggaacc agagcgtggc ccgcagcacc agccgcccgg cctcgatgag catctgcgac
     8401 tgcaggtccg cgctcacgcg gttgtcgagc ccgtcgatgg cagaccaaag cgcctcgaaa
     8461 ccgaaggcgt cgcgcaccag gatgaaggca cgcgtgacct cgtccggcgt ggcgcccgtc
     8521 tcctcctgca tgcgatgcac gaacaccgac ccggtgcggt tgatcatcga attcgcgacg
     8581 acggtcgcga tgatctcgcg cctgagcggg tggcgcggca tgacggcggc gtagcgcttc
     8641 gtgagctcgg ccggaaagta ggcgacaagt gcgcgcgcga cgaattcgtc gtcgacgagc
     8701 gtgcccttca gcaggtcgtc gtagagcacc atcttggaat aggcgagcag caccgcgcgc
     8761 tccggcgagg tgagtcctgc gcgcaacgcc cgccgctcgg cgatctcatc ttcggacgga
     8821 aggtactcga cggcgcggtt cagcctgccg gccttctcca gcgcgcggat cagggacgcc
     8881 tgcgcatcca ggagcttctc cccgcgcatc ccggaaacgg cgagcgactg cgtctggaag
     8941 tagttgtcgg cgagcaccag ccgccccacc tcgtcggtca tcgcggcgag cagcttgttg
     9001 cgctgctttt cggtgagctc cccgtcggcg accaccagcc cgaggaggat cttgatgttc
     9061 acctcgtggt cggagcagtc cacgcccgcc gagttgtcga tggcatcggt gcagatgcga
     9121 cccccggcca gggcgaactc gatgcgcccg agctgggtga agccgaggtt gcctccctcg
     9181 cacacgacct tgcagcgcag ctccgccgca ttcacgcgga tcgcgtcgtt ggcccggtcg
     9241 cccgcgtcgg cgtgcgtctg gcggcttgcc ttcacgtagg ttccgatgcc gccgttgtag
     9301 agcaggtcca cccgcgcctt gaggatcgcc ttcatgagct cctgcggcgt gagcgcctcc
     9361 tcgacgattc ccagcgccgt cctcgcctcg gcagaaagcg cgatcgactt ggccgtgcgc
     9421 gggtatacgc cgccgccccg ggacaggacc gaccggtcgt agtcctccca cgaggagcgc
     9481 gggagcgcga acagccgctc gcgctcccgg aagctcgccg cggtgtcggg atccgggtcg
     9541 aggaagatgt gccggtgatc aaaggccgcc accagcttga tgtggcgcga caggagcatc
     9601 ccgttgccga acacgtcgcc cgacatgtcg ccgacgccga cgaccgtgaa gtcctgcgac
     9661 tgcgtgtcca atcccgcctc gcggaaatgg cgcttcacgc tctcccaggc gccgcgggcg
     9721 gtgataccca tcttcttgtg gtcgtaaccc gcgctgccgc cggaggcaaa ggcgtcgccc
     9781 agccagtggc cgtattccgc gctcaccgcg ttggcataat cggagaaggc cgccgtgccc
     9841 ttgtccgccg ccaccacgag gtaggggtcg tcgggatcgt ggcgaacgac gtccctcggc
     9901 ggaacgatct tcccgccctg caggttgtcg gtcacgtcga gcaggccgcg caggaaatcc
     9961 tggtagcagg ccacaccttc cttgaggtag gcctcgcggt ccgatgccgg tggcgcggcc
    10021 ttgagcacga agccgccctt cgagcccacc gggacgatga ccgcgttctt caccatctgc
    10081 gccttcatca ggccgaggac ctcggtgcgg aaatcctccg ggcggtcgga ccagcggatc
    10141 ccgccgcgcg cgaccttgcc gccgcgcagg tggatcgcct cgaatcgcgg cgaatagacg
    10201 aagatctcga agagcggacg cggctcgggc aggtccggaa ccatcgcgca atcgatcttg
    10261 agcgagaggt agggcttgcg ccttcctccc gcgtcgcgca cccagtggtt ggtgcggatc
    10321 gtcgcgcgga tcgcgtacag gaagcggcgc aggatgcggt cctcgtccag gttggccacg
    10381 ccgttgagag cagccttcgc ctcgtcgtga aggcgctgct gcgccgcctc gcgatcggcg
    10441 cccgcggccg ggtcgaagcg cgcctggaaa atggccacga gcatggcgac gatggccggg
    10501 tgcgcggcca gcgtctgcgc gatgtatgcc tggctgaagg tgaagcctgc ctggcgcagg
    10561 taccgggcgt aggcgcgcag cac
//
LOCUS       DASUEV010000316        12634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_310834, whole genome shotgun sequence.
ACCESSION   DASUEV010000316 DASUEV010000000
VERSION     DASUEV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310834"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1228)
                     /locus_tag="VFV55_09960"
     CDS             complement(536..1228)
                     /locus_tag="VFV55_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX4944666.1"
                     /translation="MAETFPKSAVGERTLALKAVPFFTQMNDQELDVIRSVAVEKSYP
                     KHAVVLTEGEIGDSLFMIESGRVKVFIGDEEGREIILKLLGAGHFFGEMAMIDQQPRS
                     ASVTTLEPTTLLILSHHAFEHCVERAPRIGNMVLRVLAQRVREADRKIGTLALMDVYG
                     RVASTLLELAVNVNGKLVVDEKLSQQDLANMVGASREMVNRILKDLSDRGFIAVESKS
                     ITIINRELPPSL"
     gene            1350..2303
                     /gene="trxB"
                     /locus_tag="VFV55_09965"
     CDS             1350..2303
                     /gene="trxB"
                     /locus_tag="VFV55_09965"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX4944667.1"
                     /translation="MTTRHSRLLILGSGPAGYTAAVYAARANLKPLLITGLAQGGQLM
                     TTTDVDNWPADHAGVQGPELMERFQKHAERFETEIVFDHIHTAKLREKPITLIGDSGT
                     YTCDALVIATGASAMYLGLPSEQAYMGKGVSGCATCDGFFYKGQDVCVVGGGNTAVEE
                     ALYLANIAHHVTVIHRRDKFRSEKILQDKLLAREKEGKVTIAWNHTLDEVLGDKSGVT
                     GVRIKSTTDGKTRDLALTGCFVAIGHKPNTDLFEGQLEMKGGYIVTRTGLEGNATATS
                     IPGVFAAGDVQDHVYRQAVTSAATGCMAALDADKYLESLAT"
     gene            2300..2863
                     /locus_tag="VFV55_09970"
     CDS             2300..2863
                     /locus_tag="VFV55_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX4944668.1"
                     /translation="MKPARPVQAPADDDADLFRREMRDVSPARPSNRVAHRHGLPPPI
                     PVQRIEDERAVLEELARLAGGVDDIEIEEDHAYLRPGLPREVLRKLRRVHWVVQDELD
                     LHGLTGDEAALETSRFLADCTRRGLRCLRIIHGKGLGSHNREPVLKGRIRKLLARKSE
                     VLAFSEPPPAQGGSGAVIVLLDSRAGP"
     gene            2860..4491
                     /locus_tag="VFV55_09975"
     CDS             2860..4491
                     /locus_tag="VFV55_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX4944669.1"
                     /translation="MSAARVVAFLLAALALIVGVDNMQRPLANPDEGRYSEISREMAA
                     SGDWITPRLNGIKYFEKPPLQYWASAAAFSVLGESEFTARIYTALCGLLALIAVGFTG
                     RRLAGADSALMAVGVLLSSPYFLGLGGVITLDMGLTAWTTVAVCAFLLAVTGPPGESR
                     RWMLLAWAGMALAVLSKGLVGIVFPAAAIFLHCLLNRDWRLLAKLAWVPGIALFLAIT
                     APWFVLVSLANPEFAQFFFVHEHFDRFLTKVHRREQPWWFFLPILFVGFLPWMLTLVP
                     ASLEGWRHEAGGATFRWRRFALLWSAFLMLFFSASGSKLPAYILPVFPILALVLGDWL
                     ARTPADRLWKPVLVLAPLLVVAIAAAWGAPERASSAWARELYAAARPWILTGLALLAV
                     AMAAGAVLLRSGRKWTAIAIIVAASMLFIDFVEDGYHRLAPRQSGQEVAEVMKRHLGP
                     ATRIYSVGIYDQTIPFYLGRTVTLVDYWDEFSPGLKREPRLSLPTLEAFEVDWVRPGD
                     ALAIIHPDLHEKLTARGLAMTLLHRDERRVVVRKP"
     gene            4488..4859
                     /locus_tag="VFV55_09980"
     CDS             4488..4859
                     /locus_tag="VFV55_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX4944670.1"
                     /translation="MNAVSFSLVLTGVLLNAAAQLLLKAGTNRIGEFAFSLANIVPVG
                     LKVAASPFVLGGLACYAVSVVVWILALSRVPVSIAYPMLSIGYIVNAAAAWFLFGESL
                     TAQKLVGIGFIVVGVWLVARS"
     gene            4856..6019
                     /locus_tag="VFV55_09985"
     CDS             4856..6019
                     /locus_tag="VFV55_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX4944671.1"
                     /translation="MKPALPGAGVAPAFLPFARPAIDEAMIAAVGETLRSRWIASGPQ
                     VQAFESALSHYVGGRPVRSLTSATGAMEVALELLGVGPGDEVVTPAQSFFASANVVER
                     VGARAVFVDVDLATRNIDLGQAAAAVNARTKVLLPVHYNAPLDPDALAAFRAKHGVRI
                     VEDAALAIGSRAGSRPVGATGDLVSFSFHPNKNMTTIEGGALVVNDPGEAAIVERLRF
                     HGIARLPDGTRDVELAGGKYNLSDVSARLGLLQLARLDDWCRARERLAMHYFACLEGD
                     DLLTPDRLPPRRNPGHSWNMFTVLLPLDRMARSRKEFMDAMQREAIGIGLSYEAIHLA
                     SFYRARGWREGQFPVSERIGRETVTLPLFPEMTTGDVERVCECLRRVLRSRPG"
     gene            6025..6972
                     /locus_tag="VFV55_09990"
     CDS             6025..6972
                     /locus_tag="VFV55_09990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4944672.1"
                     /translation="MTPPKLSVVIPVYNEEAVLAALFERLYRALDALGCSYEIVFVND
                     GSRDKSAAMLREQFQKRPDVTRVILFNGNFGQHMAIMAGFEYCRGERTVTLDADLQNP
                     PEEIAKLLAKMDEGHDYVGSIRVMRQDVAWRRWASKAMNRMRERLTRIRMTDQGCMLR
                     AYDRAIVDAIRSSREVSTYIPALAYSFAHDPVEVEVAHEERAAGESKYSLYKLIRLNF
                     DLVTGFSIVPLQLFSLFGIGVSIVALVAYFVVIVKRILAGEVNGLAAIWDRDILAFFL
                     IGVLLFGLGLVGEYVGRIYQQVRERPRYLVQAVLEKREE"
     gene            6969..7916
                     /locus_tag="VFV55_09995"
     CDS             6969..7916
                     /locus_tag="VFV55_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEX4944673.1"
                     /translation="MSATAVVFAYHDVGCRCLPVLLANGVRVALVVTHRDNPGENIWF
                     GSVERLAREAGLDVATPDDPNDPAFVARLAALAPDFLFSFYYRQMLGAAVLATARRGA
                     FNMHGSLLPKYRGRVPVNWAIIHGERETGATLHEMVAKPDAGRIAGQEAVPIGDNDTA
                     AEVFGRVTQAAGRVLERTLPAILEGSAVLNPQDLRQGSYFGGRRPEDGRIDWAKRARE
                     VHDLVRAVAPPYPGAFTTLAGKRVRILRTWWSDPPPLGPGAPGTLAAADNRAWACCGD
                     GRWLEIREAEIEGAGTLAGERLAARFARDRGGMGQFTSG"
     gene            7955..9001
                     /locus_tag="VFV55_10000"
     CDS             7955..9001
                     /locus_tag="VFV55_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX4944674.1"
                     /translation="MTKRICILGVNGFIGHHLSQRILADTDWDVYGMDMGSDRVSDLL
                     QHPRFHFFEGDITINREWIEYHIRKCDVILPLVAIATPATYVREPLKVFELDFEANLP
                     IVRSCVKYGKRILFPSTSEVYGMCRDEEFDPYASELVLGPIDKQRWIYSCSKQLMDRV
                     IWAYGSMGQLDFTLFRPFNWIGAGLDSIHTAKEGSSRVITQFFGHIVRGEPIKLVDGG
                     GQKRAFTFIDDGIAALMRIIENPGGVATGRIYNIGNPANNFSVRELATMMLELAKAYP
                     EYRDNAAQVRIVEVSSADYYGKGYQDVQNRVPKIENTMRDLGWAPKVDMATALARIFD
                     SYRASIDEARALED"
     gene            9012..9506
                     /gene="purE"
                     /locus_tag="VFV55_10005"
     CDS             9012..9506
                     /gene="purE"
                     /locus_tag="VFV55_10005"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX4944675.1"
                     /translation="MEKPLVGVVMGSQSDWDVMQHAAKVLKDFGVPYEAKVVSAHRTP
                     DAMFEYAETARARGLHCIVAGAGGAAHLPGMIASKTTLPVLGVPVPSKHLAGQDSLYS
                     IVQMPKGIPVATFAIGEAGAANAGLFAVSLLAASDAALASRLADYREELREMVAAMQL
                     PDVL"
     gene            9503..10639
                     /locus_tag="VFV55_10010"
     CDS             9503..10639
                     /locus_tag="VFV55_10010"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX4944676.1"
                     /translation="MIAPGATLGLVGGGQLGRMFTVAARTLGYRVMVLDPDPYSPAAE
                     FTTGHLNTGYTDPASLDELARTCAAVTTEFENAPAEALLVLAEKTLVRPSGNAVAVAQ
                     DRSREKGFIASHGLPVGPYADVRAAEDFDPALARVRLPAILKTARFGYDGKGQDTIAS
                     RADLVRTFAEWKGVPCVLEERLALELEISVILARAADGQVEVFPVVENRHARGILDVS
                     IAPARIPDALAAEATALATRLARSLDYVGVLAVEMFVVGGRLLVNEIAPRPHNSGHYT
                     IDACRTSQFEQQVRVLCGLPLGDPTLHTPAVMVNLLGDVWSAGEPRWEAVLRHAGAHL
                     HLYGKREARPGRKMGHVTVCEASLGQALEVAMAIRRDLGIAESA"
     gene            10690..11430
                     /locus_tag="VFV55_10015"
     CDS             10690..11430
                     /locus_tag="VFV55_10015"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX4944677.1"
                     /translation="MSRRALFAGLAALTLLPAPAAFALPGSSSIAASGKVVTESRAVR
                     GYTGIGLSVPGTVVVRQGADESLTIEADDNLMPEIETVVERGSLRIRFRRSLNVTGKS
                     TIRILVTGPAFGSLAVAGSGDIVSENLKSRSLDVSVAGSGNVKIARLEVDRLKVSIAG
                     SGDFRAAGRADEVAANIAGSGNVEAGRLEARRASVSIAGSGNATLWARESLAASLLGS
                     GDVRYHGDPAVTRSIAGSGSLKRLGPAP"
     gene            11434..12252
                     /locus_tag="VFV55_10020"
     CDS             11434..12252
                     /locus_tag="VFV55_10020"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX4944678.1"
                     /translation="MFGWFGKRDDEAKPIDEGLWESATVPYLFMRGLPPADGERLRGI
                     AGRFLARKHFFGTHDLDITPLMHVQVAAQASILVLELGLEWYDGWSEIIVYPGQFVPE
                     REIVDEAGVVHLVNDPMAGEAWLGGPVILSYEDVAMTSDEDSRVAGYNVVIHEFAHKL
                     DMLNGNPNGCPPLHPGMALADWRRAFSGAYEDFCRRVDLADRLAEEDDGAALDALPID
                     PYASESPGEFFAVASEAFFETPEWLEPAYPAVYAQLRGFYRQDPLARLPAAPGA"
     gene            complement(12273..12410)
                     /locus_tag="VFV55_10025"
     CDS             complement(12273..12410)
                     /locus_tag="VFV55_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944679.1"
                     /translation="MLLAAFYVFAIAAIILHYSGHLKRWNCEWVLIVLAIAVFPAVLF
                     L"
     gene            complement(12461..>12634)
                     /locus_tag="VFV55_10030"
     CDS             complement(12461..>12634)
                     /locus_tag="VFV55_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX4944680.1"
                     /translation="LPKVKVEVVVGDGMVEKAIDAIVKAARTGKIGDGKIFVCDVPQV
                     VRIRTGETGEDAV"
BASE COUNT         1923 a         4479 c         4173 g         2059 t
ORIGIN      
        1 agcgtgaccg tcggatgggt gcccggaaag tcgagtccag ccaaacacgt ctggcctcga
       61 ctttccaccc atctaatgac gaggccgctg cgctgcctcg tccgcgacgg tcgcgtgcca
      121 aggattgcga tcgacttcca ccgccgccgc ctcccacgct ccgtggatgc agcaccgagt
      181 gaggccacat tggaccgcgc ccttatctgc tgtcgaatgc gccacgctgc ttcctgtgcg
      241 ggccggggat ggggcaagtt aatcggcgtc cttgggcacg cgaccgtcgc gatgggagag
      301 cgcagcggcc ccatctctca atgggtaaaa agtcggggac agacgtgttt ggctggcccc
      361 gactttttgg gacccattcg acggtcactc ttgcgtgcca ggacgccgat agacttgccc
      421 cagccccggc ccgactcccc ttcgccgttc acgttcgcgt ggccaggacg cggagagaca
      481 tgccccgtcc ccggcctgca tcccgatggc accggcaaga cggctatcct gcgagctaga
      541 gggagggggg aagctcgcgg ttgatgatcg tgatggactt cgactccacc gcgatgaacc
      601 cgcgatcgga gaggtccttg aggatgcggt tcaccatctc ccgcgaggcg cccaccatgt
      661 tggcgaggtc ctgctgcgag agcttctcgt ccacgacgag cttgccgttt acgttcacgg
      721 cgagttccag cagcgtgctc gccacgcgcc cgtagacgtc catgagggcg agcgtcccga
      781 tcttgcggtc cgcctcgcgc acgcgctgcg cgagcacccg caggaccatg ttcccgatgc
      841 gcggcgcccg ctcgacgcag tgctcgaagg cgtggtgcga aaggatgagc agggtcgtgg
      901 gctccagggt cgtcaccgaa gccgaccgcg gctgctggtc gatcatcgcc atctcgccga
      961 agaagtgccc ggcgccgagc agcttgagga tgatctcccg gccctcctcg tcgccgatga
     1021 agaccttcac ccgtcccgac tcgatcatga agagcgaatc cccgatctcg ccctccgtca
     1081 ggacgacggc gtgcttgggg tagctcttct cgacggccac ggagcggatc acgtccagct
     1141 cctggtcgtt catctgcgtg aagaacggta ccgccttgag cgccagcgtg cgctcgccga
     1201 cggcggactt cgggaaggtt tcggccattt acgaggacta tagccaaaag gccttgcctg
     1261 tcaattgttt gatctcaaca ggtcacgcag tgggataatg tcgtttcccg ggcgccgatc
     1321 gcgcgctcgc cattgccttt cgcgccccca tgaccacccg acacagccgc cttctcatcc
     1381 ttggatccgg cccggccggc tataccgccg cggtctacgc cgcccgggcg aacctcaagc
     1441 ccctcctcat caccggcctg gcccagggcg gccagctcat gaccaccacc gacgtggaca
     1501 actggcccgc ggaccacgcc ggcgtgcagg gccccgagct gatggagcgc ttccagaagc
     1561 acgccgagcg cttcgaaacc gaaatcgtct tcgaccatat ccacacggcg aagctgcgcg
     1621 agaaaccgat caccctgatc ggcgactcgg gcacctacac gtgcgatgcg ctcgtgatcg
     1681 ccacgggcgc atccgccatg tacctgggcc ttccctccga gcaggcctac atgggcaagg
     1741 gcgtctccgg ctgcgcaacc tgcgacggat tcttctacaa gggccaggac gtctgcgtcg
     1801 tcggcggcgg caacaccgcg gtcgaggagg cgctctacct ggccaacatc gcgcaccacg
     1861 tgacggtgat ccatcggcgc gacaagttcc gctccgagaa gatcctgcag gacaagctcc
     1921 tcgcgaggga gaaggagggc aaggtcacga tcgcctggaa ccacacgctc gacgaggtcc
     1981 tcggcgacaa gagcggcgtc accggcgtgc gcatcaagtc cacgaccgac ggcaagaccc
     2041 gcgacctcgc gctgaccggc tgcttcgtcg ccatcgggca caagcccaac accgaccttt
     2101 tcgaggggca gctcgagatg aagggcggct acatcgtcac gcgcacgggg ctggagggca
     2161 atgccacggc caccagcatc cccggcgtgt tcgccgcggg cgacgtgcag gatcacgtct
     2221 accggcaggc cgtgaccagc gccgccaccg gctgcatggc ggcactcgac gccgacaagt
     2281 acctggagag cctcgcgact tgaaacccgc ccgcccggtc caggcgccgg cggatgacga
     2341 tgccgacctc ttccgccgcg agatgcgcga cgtgtcgccg gcacgccctt cgaaccgcgt
     2401 ggcgcaccgc cacggcctcc cgccgcccat accggtccag cgcatcgagg acgagcgcgc
     2461 cgtgctcgag gaactggcgc gcctcgccgg cggagtcgac gacatcgaga tcgaggagga
     2521 ccacgcctac cttcggcccg ggctgccgcg cgaggtgctg cgcaagctcc gccgcgtgca
     2581 ctgggtggtg caggacgaac tcgacctgca cggcctcacc ggcgacgagg cggccctcga
     2641 gacctcgcgc ttcctcgccg attgcacccg ccgcgggctg cgctgcctgc ggatcatcca
     2701 cggcaagggc ctgggatcgc acaatcgcga gccggtgctc aaggggcgca tccgcaagct
     2761 cctggcgcgc aagtccgagg tgctcgcgtt ttccgagccg ccgccggccc agggcggcag
     2821 cggggcggtg atcgtgctcc tcgactcgag ggcaggtccg tgagcgcggc gcgggtcgtc
     2881 gccttcctcc tcgcggcgct ggcgctgatc gtcggcgtgg acaacatgca acgcccgctc
     2941 gcgaacccgg acgaagggcg ttactcggag atatcgcgcg agatggccgc gagcggcgac
     3001 tggatcacgc ccaggctcaa tggcatcaag tatttcgaga agccaccgtt gcagtactgg
     3061 gcaagcgccg cggcattctc cgtcctcggg gaaagcgagt tcacggcgcg catctacacc
     3121 gcgctttgcg gtctcctcgc actcattgcg gtggggttca cggggaggcg gctcgcgggc
     3181 gcggattcgg cgctcatggc agtcggcgtc ctgctgtcga gtccctattt cctggggctg
     3241 ggaggcgtga tcaccctcga catgggcctc accgcgtgga cgacggtcgc ggtgtgcgcc
     3301 ttcctcctcg cggtgaccgg cccgccgggc gaaagccggc gctggatgct cctcgcctgg
     3361 gcggggatgg cgctcgcggt cctttccaag ggactcgtgg gcatcgtctt cccggcggcc
     3421 gcgatcttcc tgcactgcct cctgaaccgc gactggcggc ttctcgcgaa gctggcgtgg
     3481 gtgccgggca tcgcgctctt cctcgcgatc accgcgccgt ggttcgtcct ggtgtcgctc
     3541 gccaaccccg agttcgcgca gttcttcttc gtccacgagc acttcgatcg cttcctcacc
     3601 aaggtgcacc gccgcgagca gccgtggtgg ttcttcctgc cgatcctctt cgtcggcttc
     3661 ctgccgtgga tgctcacgct cgtcccggca tccctcgagg gctggcggca cgaggcggga
     3721 ggagcgacat tccggtggcg gcgcttcgcc ctgctatggt cggcgttcct gatgcttttc
     3781 ttcagcgcgt cgggatcgaa gctgcccgcc tacatcctgc cggtctttcc gatactggcg
     3841 ctcgtgctgg gcgactggct cgcgcgcacg ccggcggaca ggctctggaa gcccgttctc
     3901 gtcctggcac ccctcctcgt cgtggccatc gcggcggcat ggggagcgcc cgagcgggct
     3961 tcgagtgcct gggcgcggga gctctacgct gccgcgcgtc cgtggatcct caccggcctg
     4021 gccctgctcg cggtcgccat ggcggccggt gcggtcctgc tgcgctccgg aaggaaatgg
     4081 accgccatcg cgatcatcgt cgccgcctcg atgctgttca tcgacttcgt cgaggacggc
     4141 taccacaggc tcgcgccgcg ccagtccggg caggaggtcg ccgaggtgat gaagcgccat
     4201 ctcgggcccg ccacgcgcat ctactcggtc gggatctacg accagacgat cccgttctat
     4261 ctcgggcgca cggtcacgct ggtcgactac tgggacgagt tctcgcccgg cctgaaacgg
     4321 gagccgcggc tgtcgttgcc cacgctcgag gcgttcgagg tcgactgggt gcgaccgggg
     4381 gatgcgctgg ctataatcca tcccgacctc cacgagaagc tcaccgcacg gggcctcgcg
     4441 atgaccctcc tgcaccgcga cgagcgccgc gtcgtcgtcc gcaagccgtg aacgccgtca
     4501 gtttttccct cgtgctcacc ggcgtcctgc tcaatgccgc cgcccagctc ctcctcaagg
     4561 cggggaccaa ccgcatcggg gaattcgcct tttcgctggc gaacatcgtc ccggtgggtc
     4621 tcaaggtggc ggccagtccc ttcgtgctcg gcgggctggc ctgctacgcc gtgagcgtgg
     4681 tggtgtggat cctggcgctg tcgcgcgtgc cggtatccat cgcctacccg atgctctcca
     4741 tcggctacat cgtgaacgcc gcggcggcct ggttcctgtt cggcgaatcg ctgacggccc
     4801 agaagctggt cggcatcggc ttcatcgtcg tgggcgtctg gctggtggcc cgctcgtgaa
     4861 gcccgcactc cctggcgcag gcgtcgcccc cgcgttcctg cccttcgcga ggccggcaat
     4921 cgacgaggcc atgatcgcgg ctgtcggcga aacgctgcgc agccgctgga tcgccagcgg
     4981 gccgcaggtg caggcgttcg agtcggccct gtcgcactac gtggggggcc ggccggtgcg
     5041 cagcctcacc tccgccaccg gcgcgatgga agtggcgctc gagctcctcg gcgtcggtcc
     5101 cggcgacgag gtggtgaccc cggcgcagag cttcttcgct tccgccaacg tggtcgagcg
     5161 cgtcggcgcg cgcgccgtgt tcgtcgacgt ggacctcgcc acgcgcaaca tcgacctcgg
     5221 ccaagccgcg gccgcggtga acgcgcgcac gaaggtcctg ctgccggtgc actacaacgc
     5281 gccgctcgac ccggacgcgc tggccgcctt ccgtgcgaag cacggcgtgc gcatcgtcga
     5341 ggatgctgcc ctcgccatcg gatcgcgcgc aggttcccgg cccgtcggcg ccacgggcga
     5401 cctcgtctcg ttcagcttcc atcccaacaa gaacatgacc acgatcgagg gcggcgccct
     5461 ggtcgtgaac gacccgggcg aggcggcgat cgtggagcgg cttcgcttcc acggcatcgc
     5521 gcgcctgccc gatggaacgc gcgacgtgga gttggcgggc ggcaagtaca acttgtccga
     5581 cgtctcggcg cggctgggcc tgctgcagct cgcgcgcctc gacgactggt gccgcgcccg
     5641 cgagcgcctc gccatgcact atttcgcctg cctcgagggc gacgatctcc tcacgccgga
     5701 tcgcctgccg ccgcgccgca atcccggcca cagctggaac atgttcaccg tgctccttcc
     5761 cctggaccgg atggcgcgct cgcgcaagga attcatggac gccatgcaac gcgaagccat
     5821 cggcatcggc ctttcctacg aggcaatcca cctcgcgtcg ttctaccgcg ccaggggctg
     5881 gcgcgagggg cagttcccgg tgtcggagcg catcggccgc gagactgtca cgctgccgct
     5941 cttccccgag atgacgacgg gcgacgtgga gcgggtgtgc gaatgccttc gtcgcgtcct
     6001 gcgttcccgc cccggatgat cccgatgacc ccgcccaagc tttccgtcgt cattcccgtc
     6061 tacaacgagg aagccgtgct cgcggccctc ttcgagcgcc tctaccgggc gctggacgcg
     6121 ctgggctgca gttacgagat cgtcttcgtg aacgacggca gccgcgacaa gtcggccgcg
     6181 atgctgcgcg agcagttcca gaagcgcccc gacgtgacgc gcgtgatcct cttcaacggc
     6241 aacttcggcc agcacatggc gatcatggcc ggcttcgagt actgccgcgg cgagcgcacg
     6301 gtgacgctgg acgcggacct gcagaacccg cccgaggaga tcgcgaagct gctcgcgaag
     6361 atggacgagg gccacgacta cgtgggctcc atccgcgtga tgcggcagga cgtcgcgtgg
     6421 cgtcgctggg cgtcgaaggc gatgaaccgg atgcgcgagc gcctcacccg catccgcatg
     6481 accgaccagg gctgcatgct gcgcgcctac gaccgcgcga tcgtggatgc gatccgctcc
     6541 tcgcgcgagg tgagcaccta tatccccgcg ctcgcctact ccttcgccca tgacccggtg
     6601 gaagtcgagg tcgcccacga ggagcgcgcc gccggcgagt cgaagtactc gctgtacaag
     6661 ctcatccgcc tgaacttcga cctcgtcacc ggattctcca tcgtgccgct gcagctcttc
     6721 tcgctcttcg gcatcggcgt ctccatcgtg gcgctcgtcg cctatttcgt ggtgatcgtg
     6781 aagcgaatcc tcgccggcga ggtcaacggc ctcgcggcga tctgggatcg cgacatcctc
     6841 gccttcttcc tcatcggggt cctcctcttc ggcctcgggc tcgtgggcga gtacgtgggc
     6901 cgcatctacc agcaggtccg cgagcggccg cgctacctcg tccaggccgt cctggagaaa
     6961 cgcgaagagt gagtgccacc gcggtcgtct tcgcctacca cgacgtgggc tgcaggtgcc
     7021 tgcccgtatt gctcgcgaat ggcgttcgcg tggcgctcgt ggtcacccat cgcgacaatc
     7081 ccggcgagaa catctggttc ggcagcgtcg agcggctggc gcgcgaagcc gggctcgacg
     7141 tggctacccc cgatgatccg aacgaccccg cattcgtcgc gcggctcgcg gcgctggcgc
     7201 ccgatttcct gttttccttc tactaccgcc agatgctcgg cgccgccgtg ctggccaccg
     7261 cccggcgcgg cgccttcaac atgcacggct cgctcctgcc caagtaccgc ggccgcgtgc
     7321 cggtcaactg ggccatcatc cacggcgagc gcgagacggg cgccacgctc cacgagatgg
     7381 tcgcgaagcc cgatgccggg cgcatcgccg gccaggaggc cgtgcccatc ggcgataacg
     7441 acaccgccgc ggaagtcttc ggccgggtga cgcaggcggc cgggcgggtt ctcgagcgga
     7501 cccttccggc gattctcgaa ggctcggcag tgcttaatcc ccaggacctg cggcagggaa
     7561 gctacttcgg cgggcgcagg cccgaggatg ggcgcatcga ctgggcgaag cgcgcccgcg
     7621 aggtgcacga cctggtccgg gccgtggcgc ccccctatcc cggcgccttc actacgctcg
     7681 ccggcaagcg cgtgcgcatc ctgcgcacct ggtggtccga tccgccgccg ctcggtcccg
     7741 gcgcgcctgg aacgctcgcc gccgccgaca accgggcgtg ggcctgctgc ggcgacgggc
     7801 gctggctgga gatccgcgaa gcggagatcg agggcgcggg caccctggcc ggggagcggc
     7861 tcgccgcgcg ctttgcccgc gatcgcggcg gcatgggaca attcacttcc ggctgacgcc
     7921 acccgcgcgc cgccgatcct ccaagaaccg acaaatgacc aagcgcatct gcatcctcgg
     7981 cgtgaacggc ttcatcggcc atcacctctc ccagcgcatc ctcgccgaca ccgactggga
     8041 cgtctacggc atggacatgg gctcggaccg ggtgtccgac ctcctgcagc acccgcgctt
     8101 ccacttcttc gagggcgaca tcaccatcaa ccgcgagtgg atcgagtacc acatccgcaa
     8161 gtgcgacgtg atcctgccgc tggtggcgat cgccacgcca gcgacctacg tgcgcgaacc
     8221 gctcaaggtg ttcgagctgg acttcgaggc caacctcccg atcgtccgtt cgtgcgtgaa
     8281 atacggcaag cgcatcctct tcccctcgac atccgaggtc tacgggatgt gccgcgacga
     8341 ggagttcgac ccctacgcct cggagctggt gctggggccc atcgacaagc agcgctggat
     8401 ctactcctgc tccaagcagc tcatggaccg cgtgatctgg gcctacgggt ccatggggca
     8461 gctcgatttc acgctcttcc gcccgttcaa ctggatcggc gccggcctcg actcgatcca
     8521 cacggccaag gaagggtcgt cgcgcgtcat cacgcagttc ttcggccaca tcgtgcgcgg
     8581 cgagcccatc aagctggtgg acggcggcgg ccagaagcgc gccttcacct tcatcgacga
     8641 cggcatcgcc gcgctcatgc gcatcatcga gaacccgggc ggcgtcgcca cgggacgcat
     8701 ctacaacatc ggcaaccccg ccaacaactt ctcggttcgc gagctcgcga cgatgatgct
     8761 ggagctcgcg aaggcctacc ccgagtaccg cgacaacgcc gcgcaggtga ggatcgtcga
     8821 ggtgagctcc gccgactact acggcaaggg ctatcaggac gtgcagaacc gcgtgccgaa
     8881 gatcgagaac acgatgcggg acctcggctg ggcgccgaag gtggacatgg ccacggccct
     8941 cgcgcgcatc ttcgattcct accgcgcgtc gatcgacgaa gcgcgtgcgc tcgaagactg
     9001 aggcccggcc catggaaaag ccactcgtcg gcgtggtgat gggttcgcag agcgactggg
     9061 acgtgatgca gcacgcggcc aaggtgctca aggatttcgg cgtgccttac gaggcaaagg
     9121 tggtttccgc gcaccgcacg ccggacgcga tgttcgagta tgcggagacc gcccgcgcgc
     9181 gcgggcttca ctgcatcgtg gccggcgcgg ggggagcagc gcacctgccc ggcatgatcg
     9241 cctccaagac gacgcttccg gtcctgggcg ttcccgtgcc ctcgaagcat ctcgccgggc
     9301 aggactcgct ttactccatc gtgcagatgc ccaagggcat cccggtcgcc accttcgcca
     9361 tcggcgaggc gggcgccgcc aatgccggac tgttcgcggt gagcctgctc gccgcctcgg
     9421 atgccgcgct cgcgtcccgt ctcgcggact accgcgagga actgcgcgag atggtcgcgg
     9481 cgatgcaact ccccgacgtg ttgtgatcgc gcccggcgcc acgctcgggc tggtgggtgg
     9541 cgggcagctc ggccgcatgt tcacggtggc ggcgcgcacg ctgggctacc gcgtcatggt
     9601 cctcgacccc gatccctact cgccggccgc cgaattcacc accgggcacc tgaacaccgg
     9661 ctacacggac cctgcgtcgc tcgacgaact cgcccggacc tgcgcggcgg taaccacgga
     9721 attcgagaac gctcccgccg aggcgctgct cgtgctcgcc gagaagacgc tcgtgcgccc
     9781 cagcgggaat gcggtggcgg tggcgcagga tcgcagccgc gagaagggct tcatcgcctc
     9841 ccatggcctg cccgtgggcc cgtacgccga cgtgcgcgcc gcggaggatt tcgatccggc
     9901 gctcgcccgc gtgaggctcc ctgccatcct caagaccgcg cgcttcggct acgacggcaa
     9961 ggggcaggac acgatcgcct cccgggccga cctcgtgcgc acgtttgccg agtggaaggg
    10021 cgtgccctgc gtgctcgagg aacgcctggc actcgagctc gagatctcgg tgatcctggc
    10081 gcgcgccgcc gatggccagg tcgaggtctt cccggtggtg gagaatcgcc acgcgcgcgg
    10141 aatcctcgac gtctccatcg caccggcgcg catcccggat gcgctcgccg ccgaggcgac
    10201 cgcactcgcg acccgcctcg cgcggtcgct ggactacgtg ggcgtgctcg ccgtcgagat
    10261 gttcgtcgtg ggcgggcgtc tcctcgtgaa cgaaatcgcc ccccggccgc acaacagcgg
    10321 ccactacacg atcgacgcct gccgcacctc gcagttcgag cagcaggtgc gcgtgctgtg
    10381 cgggctgccg ctgggcgacc ccacgctgca cacccccgcg gtgatggtca acctgctggg
    10441 cgacgtctgg agcgcgggcg aaccgcgctg ggaggcggtg ctgcgccacg cgggcgcgca
    10501 cctgcacctg tacggcaagc gcgaggcacg cccgggccgg aagatgggcc acgtcaccgt
    10561 ctgcgaggcg agcctcgggc aggccctgga ggtcgcgatg gccatccgcc gggacctggg
    10621 aatcgccgaa tccgcctgac gggctccgtg cgtcaaacgg gaacctgccc attctccgga
    10681 gccctcatga tgagccgccg cgccctcttc gccggccttg ccgcgctgac cctgcttccc
    10741 gctcccgccg cgttcgccct ccccggttca tcctcgattg cggcgtccgg caaggtggtg
    10801 acggaatcgc gcgcggtgcg cggctacacg gggatcggcc tttccgtgcc cgggacggtc
    10861 gtggtccgcc agggcgccga cgaatcgctg acgatcgagg ccgacgacaa cctcatgccc
    10921 gagatcgaga cggtggtcga acgcggctcg ctcaggatca ggttcaggcg ttcgctcaac
    10981 gtcaccggaa agtccaccat ccgcatcctc gtcacgggac ccgccttcgg ctccctcgcg
    11041 gtagccggca gcggcgacat cgtctcggag aacctgaagt cgcgctccct cgacgtgtcc
    11101 gtcgccggct ccgggaacgt gaagatcgcc aggctcgagg tcgaccgcct caaggtgtcg
    11161 atcgccggca gcggcgattt ccgcgcggcc gggcgcgccg acgaagtggc cgcgaatatc
    11221 gcgggctccg gcaacgtcga ggccggccgg ctcgaagcac ggcgcgcgag cgtttcgatc
    11281 gccggctcgg gtaatgccac tctctgggca cgcgaatcgc tggctgccag cctgctcggc
    11341 agcggcgacg tccgctacca tggcgatccc gcggtcacga ggagcatcgc gggctccggg
    11401 tcgttgaagc gcctcgggcc cgcgccctga ggcatgttcg gctggttcgg aaaacgcgac
    11461 gacgaggcga agcccatcga cgaggggctc tgggaaagcg ccacggtccc ctacctgttc
    11521 atgagggggc tgccgcccgc cgacggcgag cggctgcgcg ggatcgccgg ccggttcctc
    11581 gcccgcaagc acttcttcgg cacccacgac ctggacatca cgcctctcat gcacgtgcag
    11641 gtggcagcgc aggcgtcgat cctggtcctc gagctcggtc tcgagtggta cgacggctgg
    11701 agcgaaatca tcgtctatcc gggccagttc gtgccggagc gcgagatcgt cgatgaggcc
    11761 ggcgtcgtgc acctcgtcaa cgacccgatg gccggggaag cctggctcgg cgggccggtg
    11821 atcctctcct atgaggatgt cgcgatgacg agcgacgaag actcgcgcgt cgccggctac
    11881 aacgtggtga tccacgagtt cgcgcacaag ctcgacatgc tgaacgggaa cccgaatggc
    11941 tgcccgccgc tgcaccccgg gatggctctc gcggactggc ggcgggcgtt cagcggggcc
    12001 tacgaggatt tctgccggcg cgtggacctg gccgaccgcc tggcggaaga agacgacggg
    12061 gcggcgctcg acgcgctgcc gatcgacccc tacgcctcgg agagtcccgg ggaattcttc
    12121 gcggtggcat cggaggcgtt cttcgaaacg ccggaatggc tcgaacccgc ctatccggcg
    12181 gtgtacgcgc agctacgggg attttatcgg caggacccgc tggcgaggct gccggcggca
    12241 ccgggcgcct gagacgccgc cgggcgctgc ggtcagagga agaggaccgc gggaaatacg
    12301 gcgatcgcga ggacgatcag cacccactcg cagttccacc gtttcaggtg gccgctgtag
    12361 tgcaggatga tcgcagcgat ggcaaagacg tagaaagcgg caagaagcat cattcccccc
    12421 ttattttgcc gcaaaggata gcaggaaagt cccccggggc ctagaccgcg tcctcgcccg
    12481 tttcgccggt gcggatccgc accacctgcg gaacatcgca gacgaagatc ttgccgtcgc
    12541 cgatcttgcc ggtgcgggca gccttcacga tcgcgtcgat ggccttttcc accatcccgt
    12601 cgccgaccac gacctcgacc ttcaccttgg gcag
//
LOCUS       DASUEV010000317         7169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1477008, whole genome shotgun sequence.
ACCESSION   DASUEV010000317 DASUEV010000000
VERSION     DASUEV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477008"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="VFV55_10035"
     CDS             complement(<1..1791)
                     /locus_tag="VFV55_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEX4944681.1"
                     /translation="MATSQKTFTQQLKKYYTFYTGGFILFVVALAIGEKLGMTPKWIG
                     YCFLVFTILLYAGIGIMSRTLEVAEYYVAGRRVPAFFNGMATGADWMSAASFIGMAGT
                     LYLTGFQGLSFIMGWTGGYVLVALLLAPYLRKFGQFTIPDFLGARYGGNIVRSLGVIA
                     AIIASFTYVVAQIYGVGLITSRFTGLEFGIGVFVGLAGILVCSFLGGMKAVTWTQVAQ
                     YVILIIAYMIPVVWLSVKHTGIPLPQLVYGKVLEKVTEREKVLTNDPGEKQVRQIFAD
                     RAKAADDRIKGLPASYQTEKDSVTRKLEELKAAAAPKAELDAAEKAVKDFPASPDAAK
                     AKWTADKAGAAGRTGPPLPHATPYPGKDEAARDNAKWNFIGVVFCMMFGTAALPHILM
                     RYYTTPSVQEARVSVFWSLFFIFLLYFTAPALAVLAKFDVYTLIVGSKFSDLPAWVNA
                     WRTVDPTLLNITDINKDGIVQLAEIVIGGDLIVLATPEIAGLPYVISGLVAAGGLTAA
                     LSTADGLLLTIANALSHDLYYKMINPNASPGLRITVSKILLLGVAVVAAYVTSLKPGN
                     ILFLVGAAFSIAASAFFPALVMGIFWKRASK"
     gene            complement(1791..2057)
                     /locus_tag="VFV55_10040"
     CDS             complement(1791..2057)
                     /locus_tag="VFV55_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX4944682.1"
                     /translation="MQLTQNHIDYWHKNLRLTAVLLVIWFIATFVLAWYAIPLAEFKF
                     FGWPFSFYMAAQGSLIIYVAIIGYYAIAMRKADLEHGVHEGEED"
     gene            complement(2341..3024)
                     /locus_tag="VFV55_10045"
     CDS             complement(2341..3024)
                     /locus_tag="VFV55_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4944683.1"
                     /translation="MNSMPPLAAADPSEPTVYVIDDDESIRELLTWLMKRNAIRAEAF
                     PNAKSFLKACRPGARGCLILDLYMPGMSGLDLQQYLKEVGIEMPVIFLSGRADVPKAV
                     LAVKSGAIDFIEKPFDYRQIVDLVRECIRRDGESRAGRDAARERAGRLATLTQREREV
                     LDRVIAGKVNRLIAEEMRISIKTVEAHRARIMEKLAVDSVAELVQATIEARTHERNGG
                     RGGGPGSAG"
     gene            complement(3021..3434)
                     /locus_tag="VFV55_10050"
     CDS             complement(3021..3434)
                     /locus_tag="VFV55_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4944684.1"
                     /translation="MAKRVLIAEDEPSIVTSLEFLMRQGGFETCTAADGEEALSLLAI
                     FRPHLVLLDIMLPRRSGLELCRLIRADASLAGTRVLMLTAKGGHRDIERGLEAGADDY
                     VTKPFSTHDLVARAQALLADPVLRLAPGAAPRRTS"
     gene            complement(3443..6208)
                     /locus_tag="VFV55_10055"
     CDS             complement(3443..6208)
                     /locus_tag="VFV55_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4944685.1"
                     /translation="MLKGWIIITASFAYIGLLFAIAWFADRRAAQGRSIISNAWVYSL
                     SLAVYATAWTFYGSVGRAATDGVGFLPIYLGPTLTMLLGWFVLRKMVRIVRLNRTTSL
                     ADFVASRYGKSALIGGLVTVIAVIGILPYISLQLKAVSTSFQILLGYPEIVMPPRVGS
                     MVVMQDTAFYVALFMALFAIAFGTRQLDAAERHEGMVAAIAFESIVKLIAFLSVGIYV
                     AFFMYDGLGDLFARAAGRPDLEVLMAPLGGAAGSYASWVWLTVLSMFAVIFLPRQFQV
                     AVIENTDERHLKRAAWLFPLYMLAINVFVLPIAFGGVLHFPQGDVDADTFVLTLPMTQ
                     KAEGLALLVFIGGLSAATGMVIVETIALSTMVCNDLVMPLLLRIRAFRLTEQRDLSSL
                     VLDIRRAAIIALLMGGYFYYLLAGEAYALVSIGLISFAAAAQFAPAVIGGLYWKGGTR
                     TGALAGLAAGFSVWIYTLLLPAFAKSGWLGVELVERGPWGIELLRPTQLFGLAGLDQI
                     THAMIWSMIANAGAYMVGSVIRRQSAAEHVQATLFVDVYQQTGEPGSQWRGAMSASAL
                     QTLVGRFLGPARAVELFTAYARARGVTSVDELEGDRDLVRFAELQLAGTIGAASARTM
                     VATVAQEEPLGLEEVMTIIDEASQVIAYSHSLEEKQRELETATQELKAANERLKELDR
                     LKDDFISTVTHELRTPLTSIRAFSEILHDNPDLERAERQRFLALVIKESERLTRLINQ
                     VLDLAKIESGMAEWRTAELDMREIVLEAMNATSAIFKSRGVQVETRIALAVPLVLADR
                     DRLMQVLLNLLSNAVKFCTPGKGRVAVRLSCDGAAVRVDVEDNGVGISPENQALIFEK
                     FRQVGDSLTEKPAGTGLGLAICRGIVGHFGGRLWVESEPGRGSVFSFDLPASAAAGAA
                     GRLVANA"
     gene            complement(6219..6401)
                     /locus_tag="VFV55_10060"
     CDS             complement(6219..6401)
                     /locus_tag="VFV55_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944686.1"
                     /translation="MRAYRIVALFLLGCLLFNYPVLALFNVGATAAGVPVLYVYLFVA
                     WAGTIALAAIVIEGGS"
     gene            complement(6524..>7169)
                     /locus_tag="VFV55_10065"
     CDS             complement(6524..>7169)
                     /locus_tag="VFV55_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX4944687.1"
                     /translation="VVRRIERWRSLADFERGRPPSSGRWVVVDVESSGLDAVSDSLIA
                     VGALAVVDGGIDLADSFEVILRQDAPSTVDNIEVHGIGGMEQTGGEDPGEALAAFLAF
                     IGKDPLVAFHAPFDSTMLRRAIDRHLGVAFRRPWLDLADIAPLVWPKYASRLSGLDDW
                     LETFLIPADFRHRAIADCLATAQLLLMVLPASAGIGASTAGELLSLSGADRWLS"
BASE COUNT         1267 a         2364 c         2429 g         1109 t
ORIGIN      
        1 cttcgacgca cgcttccaga agatgcccat caccagcgcc gggaagaacg ccgaggccgc
       61 gatcgagaac gccgcgccga cgaggaacag gatgttcccc ggtttgagcg acgtcacgta
      121 ggccgcgacg accgctacgc cgagcagcag gatcttcgac accgtgatgc gcaaccccgg
      181 ggacgcattg gggttgatca tcttgtaata gagatcgtgc gacagggcat tggcgatcgt
      241 gagcagcaac ccgtcggcgg tcgaaagcgc cgcagtgagg ccaccggcgg ccaccaggcc
      301 cgagatcacg tacggcaggc ccgcgatttc cggggtcgcc agcacgatca ggtcgccgcc
      361 gatcacgatt tcggcgagtt gcacgatgcc gtccttgttg atgtcggtga tgttgagaag
      421 cgtcgggtcc accgttcgcc atgcgttcac ccaggccggc aagtccgaga acttcgatcc
      481 cacgatcagg gtgtacacgt cgaacttggc cagcaccgcg agcgcgggcg ccgtgaagta
      541 gagcaggaag atgaagaaga gcgaccagaa caccgacacg cgcgcttcct gaaccgaagg
      601 cgtggtgtag tagcgcatca ggatgtgcgg cagggcagcg gttccgaaca tcatgcagaa
      661 gacgacgccg atgaagttcc acttcgcgtt gtcgcgcgcg gcttcgtcct tgccgggata
      721 cggcgtcgcg tgcgggaggg gaggaccggt gcggccggcg gcaccggcct tgtcggcggt
      781 ccactttgcc ttggcggcgt cgggagaagc cgggaagtcc ttcacggcct tttccgcggc
      841 gtcgagttcc gccttcgggg cggcggcggc cttcagctcc tcgagcttcc tggtgactga
      901 atccttctct gtctggtagc tcgccggcag gcccttgatc ctgtcgtcgg cggccttggc
      961 gcggtcggcg aagatctgcc ggacctgctt ctcgccgggg tcgttggtga gaaccttctc
     1021 gcgctccgtc accttctcga gaaccttgcc gtagaccagc tggggcaaag gaatgcccgt
     1081 gtgcttcacg gaaagccaga ccaccgggat catgtaggca atgatcagga tcacgtactg
     1141 cgcgacctgc gtccaggtga ccgccttcat gccaccgagg aacgagcaca ccaggatgcc
     1201 cgccaggccc acgaacacgc cgatgccgaa ctccagtccc gtgaagcggc tcgtgatcag
     1261 gccgacgccg tagatctgcg ccaccacgta ggtgaacgac gcgatgatgg ccgcgatcac
     1321 gcccagcgag cgcacgatgt tgccgccata gcgcgcgccg aggaagtcgg ggatcgtgaa
     1381 ctggccgaac ttgcgcaggt agggcgcgag aagcagcgcc acgagcacat agccgcctgt
     1441 ccagcccatg atgaacgaca gcccctggaa cccggtcagg tagagcgtgc cggccatgcc
     1501 gatgaaggag gcggcgctca tccagtccgc gcccgtcgcc atgccgttga agaatgccgg
     1561 tacgcggcgc ccggccacgt agtactccgc aacttccagc gtgcggctca tgatcccgat
     1621 gccggcgtag aggaggatcg tgaacacgag gaagcagtag ccgatccact tgggcgtcat
     1681 gcccagcttc tcgccgatgg cgagcgccac cacgaacagg atgaacccgc cggtgtagaa
     1741 ggtgtagtac ttcttcagct gctgggtgaa cgtcttctgg ctggtggcca tcagtcttcc
     1801 tccccttcgt gaacgccgtg ctcgaggtct gccttgcgca tggcaatggc gtagtagccg
     1861 atgatggcca cgtagatgat gagcgagccc tgcgcggcca tgtagaacga gaacggccag
     1921 ccgaagaact tgaattcggc aagcgggatc gcataccacg cgaggacgaa ggtggcgatg
     1981 aaccagatca cgagcaggac cgccgtgagc ctcaggttct tgtgccagta gtcaatgtga
     2041 ttctgggtaa gctgcatcgc gttcctccta attgatcgtt gtcctcggat cggtggtccg
     2101 tccgggccgg tcgagttgcc gggacgcctc gcagggcgct tgcacggcgc gggtcgtggt
     2161 cgaagcgtga aaggaaggga ctgacgaagc caccacgacc cgtaccggcg cttgcgcccc
     2221 gccatccctc ttgccgggga ttggccgggc gcggccgatt tgttcttctt gggtggccct
     2281 cctccttaaa cctgcgcgat gctaatgccc ctttccggcg gggggcaatt ggggttaacc
     2341 ctaacccgcg gagccggggc ctccgcccct gccaccgttg cgctcgtgcg tgcgcgcttc
     2401 gattgtggcc tgcacgagtt ccgcgacgga atcgacggcg agcttttcca tgatgcgtgc
     2461 gcggtgcgcc tcgaccgtct tgatcgagat ccgcatctcc tcggcgatga gacggttcac
     2521 cttgccggcg atcacgcggt ccagcacctc gcgctcgcgc tgggtgaggg tcgccaggcg
     2581 cccggcgcgc tccctggcgg catcacgacc tgcccgcgat tcgccgtcgc gccggatgca
     2641 ctcccgcacc agatcgacga tctggcgata gtcgaacggc ttttcgatga agtcgatggc
     2701 cccgctcttc accgcgagga cggccttcgg gacgtccgcc cggcccgaaa ggaagatgac
     2761 cggcatctcg atgccgactt ccttgaggta ttgctggagg tcgagcccgc tcatccccgg
     2821 catgtacagg tcgaggatga ggcagccgcg cgccccggga cggcaggctt tcaggaagct
     2881 cttcgcgttg gggaacgcct cggcgcggat ggcgttgcgc ttcatgagcc aggtgaggag
     2941 ctcgcggatc gactcgtcgt cgtcgatcac gtagacggtg ggctcggacg ggtccgccgc
     3001 ggcgaggggg ggcatggagt tcatgaggtc cgccggggcg ccgcgccagg tgcgagccgc
     3061 aggacgggat ccgcaagcag tgcttgtgcg cgcgcaacca ggtcgtgcgt ggagaacggc
     3121 ttggtgacgt agtcgtccgc acccgcctcc aggccccgct cgatgtcgcg atgcccgccc
     3181 ttggcggtga gcatgaggac gcgcgttccc gcgagcgacg catcggcgcg gatcagccgg
     3241 cagagctcca gcccgcttcg cctcggcagc atgatgtcga ggaggaccag gtgcgggcga
     3301 aagatcgcga ggagcgagag cgcctcctcg ccatcggcag cggtgcatgt ctcgaagccc
     3361 ccctgccgca tcaggaattc gagcgaagtc acgatgctcg gttcgtcctc ggcgatcagc
     3421 acgcgcttgg ccacggcgcg cctcaggcgt tggcgacgag gcgcccggcg gcgcccgccg
     3481 cagccgacgc gggcagatcg aaggagaata ccgagccgcg gcccggctcg ctctccaccc
     3541 acaatcgccc gccgaaatgt ccgacgatgc cgcggcagat ggcgagcccg aggcccgtgc
     3601 ccgccggctt ctcggtaagg gagtcgccga cctgccggaa cttctcgaag atcagcgcct
     3661 ggttctccgg gctgatgccc acgccgttgt cctcgacgtc gacccgcacc gcggcgccat
     3721 cgcacgacag gcgaaccgcg acacgcccct tccccggggt gcagaacttg accgcgttcg
     3781 acagcaggtt cagcaggacc tgcatcaggc ggtcgcggtc ggccaggacc agcggcacgg
     3841 ccagcgcgat ccgcgtttcg acctggacgc cgcgcgactt gaagatcgcg ctcgtcgcgt
     3901 tcatggcctc caggacgatc tcgcgcatgt cgagttccgc ggttcgccat tccgccatcc
     3961 cggactcgat cttcgcgaga tccagcacct ggttgataag gcgcgtgagt cgctccgact
     4021 ccttgatcac cagcgcgagg aaacgctgcc gctcggcgcg ctcgagatcg gggttgtcgt
     4081 gcaggatctc ggaaaaagcg cggatggacg tgagcggcgt gcgcagttcg tgggtgaccg
     4141 tcgagatgaa gtcgtccttg agccggtcga gctccttcag gcgttcgttg gcggccttga
     4201 gctcctgcgt ggccgtctcc agctcgcgct gcttttcctc gagcgaatgg ctgtaggcga
     4261 tcacctgcga ggcctcgtcg atgatcgtca tcacttcctc gaggcccagc ggctcctcct
     4321 gcgccaccgt ggccaccatc gtcctcgcgg acgcagcgcc gattgtgccg gcaagctgca
     4381 actcggcgaa ccgaacgagg tcgcggtcgc cttcgagctc gtcgacgctc gtcacgccgc
     4441 gcgcccgcgc ataggcggta aagagctcca cggcccgcgc cggcccgagg aaccggccca
     4501 ccagggtctg cagcgccgaa gccgacatcg ccccgcgcca ctgcgagccc ggctctcccg
     4561 tctgctggta gacgtcgacg aagagcgtgg cctgcacgtg ctcggccgcg ctctggcggc
     4621 ggatgacgga accgaccatg taggcgcccg cattggcgat catgctccag atcatcgcat
     4681 gggtgatctg gtcgagacct gccagcccga agagctgcgt gggccgcaga agctcgatgc
     4741 cccagggccc gcgctcgacg agctcgacgc cgagccagcc ggatttcgcg aaggccggca
     4801 ggagcagggt gtagatccat accgagaatc ccgcggcaag ccctgccagc gcgcccgtcc
     4861 gcgttccgcc cttccagtaa aggccgccaa tgacggccgg cgcgaactgc gcggctgcgg
     4921 cgaaggagat gagcccgatc gagacgagcg cgtaggcctc gccggcgagc aggtagtaga
     4981 aatagccccc catgaggagg gcgatgatcg ccgcgcggcg gatgtccagg accaggctgg
     5041 aaaggtcacg ctgctccgtg agccggaagg cgcggatgcg cagcagcagc ggcatgacga
     5101 ggtcgttgca taccatcgtg gaaagcgcga tcgtttccac gatcaccatg ccggtagccg
     5161 ccgagaggcc gccgatgaac acgagcaggg caaggccctc ggccttctgc gtcatcggaa
     5221 gcgtgaggac gaacgtgtcg gcgtccacat cgccctgcgg gaagtgcagc accccgccga
     5281 acgcgatcgg cagcacgaag acgttgatcg ccagcatgta cagcgggaag agccaggccg
     5341 cccgcttcag gtggcgctcg tcggtgttct cgattaccgc cacctggaac tgccggggca
     5401 ggaagatgac cgcgaacatc gaaagcacgg tgagccagac ccagctcgcg tagctgccgg
     5461 ccgcgccccc cagcggcgcc atcagcactt ccaggtccgg ccgtccggcg gcacgcgcga
     5521 agaggtcgcc cagcccgtcg tacatgaaga acgccacgta gatgccgacc gagaggaagg
     5581 cgatcagctt cacgatcgac tcgaaggcga tggcggccac catgccttcg tggcgctcgg
     5641 ccgcgtcgag ctggcgggta ccgaaggcga tggcgaagag cgccatgaag agcgcgacgt
     5701 agaaggccgt gtcctgcatc acgaccatcg agcccaccct gggcggcatc acgatctccg
     5761 ggtagcccag gagtatctgg aagctcgtcg agaccgcctt cagttgcagc gagatgtagg
     5821 gcaggatccc gatcaccgcg atcacggtga ccagcccgcc gatgagcgcg ctcttgccgt
     5881 agcgcgaggc cacgaagtcg gcgagcgagg tggtgcggtt gagccgaacg atgcgcacca
     5941 tcttgcgcag gacgaaccat cccagcagca tcgtgagcgt cgggccgagg tagatcggaa
     6001 gaaagcccac gccgtcggtg gcggcgcgcc ccacgcttcc gtagaaggtc caggccgtcg
     6061 cgtacaccgc gagcgacagg ctgtacaccc aggcgttgga gatgatcgag cggccctggg
     6121 ccgcccgccg gtccgcgaac caggcgatgg cgaagagcag cccgatgtag gcgaacgacg
     6181 cggtgatgat gatccagccc ttcagcacgg cgcttcccct agctgccgcc ttcgatcacg
     6241 atcgccgcca gcgcgatcgt tcccgcccac gccacgaaga ggtacacgta gagcacgggc
     6301 acgcccgccg ccgtggcgcc cacgttgaag agcgcgagca ccgggtagtt gaacaggagg
     6361 cacccgagga ggaacagggc cacgatgcgg tacgcgcgca tgctcggtct tctcatcgtc
     6421 tcctcgaggg cgaatgcggg cgaatccgcg gcccaccggc cgcgcgcccg gcgctagtag
     6481 agggccccgg cgcccgcctt gtcaatcgag cccgcggcga ccttcaggaa agccagcggt
     6541 ccgcgccgga aagcgaaagg agctccccgg ccgtcgaagc cccgattccg gccgaggcag
     6601 gaagcaccat gagcagcagt tgcgccgtcg cgaggcaatc ggcgatggcg cggtggcgga
     6661 agtcggccgg gatcaggaat gtctcgagcc agtcgtccag gcccgaaagc cggctcgcgt
     6721 acttcggcca cacgaggggc gcgatgtccg cgaggtcgag ccacggccgt ctgaatgcga
     6781 cgccgaggtg ccggtcgatg gcgcggcgca gcatcgtcga gtcgaacggg gcgtggaagg
     6841 cgacgagcgg gtccttgccg atgaaggcga gaaaggcggc cagcgcctcg ccgggatctt
     6901 cgcccccggt ctgctccatg ccgccgatgc cgtggacctc gatattgtcg acggtgcttg
     6961 gagcgtcctg gcgcaggatg acctcgaagc tgtccgcaag gtcgatcccg ccgtcgacga
     7021 cggccagggc gcccacggcg atgagcgagt cggagacggc gtccaggccg ctcgactcca
     7081 cgtccacgac gacccaccgt ccggaggaag gcgggcggcc gcgttcgaaa tcggcgaggc
     7141 tgcgccagcg ctcgatgcgc cgcacgacc
//
LOCUS       DASUEV010000318         7427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1599242, whole genome shotgun sequence.
ACCESSION   DASUEV010000318 DASUEV010000000
VERSION     DASUEV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599242"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VFV55_10070"
     CDS             complement(<1..815)
                     /locus_tag="VFV55_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX4944688.1"
                     /translation="MPEVVERPIDESARARLVAGGVDPRLARLFAARGVSDAAELSTA
                     LPGLAFPDRLGHAAEAAALLADAIEAGTRILIVADYDADGATACAVGLRALRAMGATV
                     DYLVPDRFRFGYGLTPEIVRIAAERSPGLLVTVDNGIASVEGVAEANRLGMRVLVTDH
                     HLPGEERPDAACIVNPNQAGCGFPSKNLAGVGVIFYVMTALRAELRRRGAFAGRAEPN
                     LAALLDLVALGTVADVVKLDANNRILVAQGLARIRAGESCAGIRALLAVAGREP"
     gene            complement(808..1806)
                     /locus_tag="VFV55_10075"
     CDS             complement(808..1806)
                     /locus_tag="VFV55_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944689.1"
                     /translation="MPLTLLVPDLLAPADAPEPMRSLRLPGLEAWLARADATREPGTG
                     SAWLLRQWGLGVQAPVAALTLAAEGGPREGAWLRADPVHQRVDRNALVLHDASVLALT
                     QEEADAAVGELNAFFAGDGLEFVAPSAERWYLRVPPEELPRTVPLEEAVGRNPFGMIP
                     EGGVRLKWPSIFSEAQMLLARLPLNAVRESEGRPTLNAVWFWGGGHYPADLPRPYACV
                     AATDALARSLALASGCAVQSLPAGAARLPSRESAQCLVVVESLRTPFRRGDMEAWLAA
                     ARILEHDWFSNLGEALAAFGSVRLVLPSERDTAVFEIARRARWRLLRRRRPLVSHA"
     gene            1869..3113
                     /locus_tag="VFV55_10080"
     CDS             1869..3113
                     /locus_tag="VFV55_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX4944690.1"
                     /translation="MQPFELFVGLRYTRAKRRNHFISFISLVSMLGIALGIAALITVM
                     SVMNGFEREIRARILGVAAHLQVLAVEGGLADWRKVAAQAKANPEVLAAAPFVAAQGL
                     LSSGSQVRGVFVRGVVPELEEKVADFGAHMRGGKLSDLQPGEFGIVLGAELARALRLQ
                     VGERVTLIAPQGQVTPAGLVPRLKQFRVVGIFSVGHFEYDSGLALVRMEDAQVLYRLG
                     DAASGVRLKLANLYEAPRVGRELAKTIEADAYVTDWTQQNANYFRAIQIEKRMMFIIL
                     TLIIAVAAFNLVSTLVMVVTDKHPDIAILRTLGASPASIMKIFMVQGTLIGVIGTGLG
                     VLLGVLLSLNIDVVVPAVERAFNFQILSREVYYISDLPSHLEWRDVWSVAVVALGLAF
                     MATLYPSWRAARVNPAEALRYE"
     gene            3106..3786
                     /gene="lolD"
                     /locus_tag="VFV55_10085"
     CDS             3106..3786
                     /gene="lolD"
                     /locus_tag="VFV55_10085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEX4944691.1"
                     /translation="MSEAVLSCAGLAKTFGLGDVAVPVLRAIDFEVGAGESVAIVGAS
                     GSGKSTLLHLLGGLDRPSAGTVRMAGEDMASLDPRRQGELRNRCLGFVYQFHHLLPEF
                     TALENVAMPLAIRRLGAAEAKARAATMLGEVGLGHRLAHRPGELSGGERQRVALARAL
                     VTQPACVLADEPTGNLDRGTAGQVFDLIVELNARHGTAFVMVTHDPELAARMDRRLRL
                     ADGRLAQD"
     gene            complement(3791..4348)
                     /locus_tag="VFV55_10090"
     CDS             complement(3791..4348)
                     /locus_tag="VFV55_10090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4944692.1"
                     /translation="MESPRLVVPSSSYKDSYRSLVAEFLARGERLIPFPLSFPNENFE
                     AFLAQLAGCEQGIGIPEGFVPHSTFWLLRGEEVVGVSNVRHRLNDRLRVEGGHIGYGI
                     RPTARGQGLGNAILRLTLAQAARLGIDHVLLTCSKDNVASSAVIAGNGGRLESEAFVE
                     ARGEVVQRWLIDIAARAEPAIGDRP"
     gene            complement(4350..4967)
                     /locus_tag="VFV55_10095"
     CDS             complement(4350..4967)
                     /locus_tag="VFV55_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX4944693.1"
                     /translation="MKPRYWDEAKTHLAKKDKVLRGIIRAHPDANLRTRGDAFRTLAR
                     SIVGQQISVRAAQAVWERFEALSGNLAPAAIVGLDEEAMRAAGLSRMKVAYLKDLAGR
                     FHEGSLRPRRWTRMDDEAVIADLVQVKGIGRWSVEMFLIFHLMRPNVLPVGDLGLQRA
                     MERLYNSGEALTRDDMREIAKPWQPWSSVATWYLWRSLDPVPVEY"
     gene            complement(4964..5521)
                     /locus_tag="VFV55_10100"
     CDS             complement(4964..5521)
                     /locus_tag="VFV55_10100"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX4944694.1"
                     /translation="MIRRKLRKWLPSAESIRGHRWLQWCAPVLTHPRLWHLHRRAVAL
                     GVAIGLVTGLIPGPVQMLVAALIAIPLRANIPAAMFTTLYTNPFTFVPLYILAYNIGG
                     LVTGGSAPLVMPPDIGWNWEGLVLLFPSLLAWIVSMGDTLLIGLAIQCTAFAIGGYAL
                     TMVAWRILVTRAWHARHARRARATP"
     gene            5642..6211
                     /locus_tag="VFV55_10105"
     CDS             5642..6211
                     /locus_tag="VFV55_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX4944695.1"
                     /translation="MKVATCFMAVLLVAGAAATQVFAQEGIPSLRGKPTKEEIARALS
                     PPAPEPVMRSRGIVLNPGAGTAAAKAASTRRAIDLEVLFEFDSARLTPDGRDVLDSLG
                     GALGSSELADVRRVTLEGHTDAKGSDAYNDSLSLLRAQSARQYLIQKHRITPDKLHAV
                     GKGKAEPADPRDPYGAANRRVRVVVEGQS"
     gene            6257..7087
                     /locus_tag="VFV55_10110"
     CDS             6257..7087
                     /locus_tag="VFV55_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944696.1"
                     /translation="MPKKNLQLIAALVAAVFAAGAQAQQTGSKVEVDKVEQRQTGKDN
                     KQFLDLGNVERGGKSDVKAGTVKQMQGQGQGNVQKMGVGNVAGTGSSKVKTGDITQKQ
                     MGGQGNTQTMGVGNVAGTGKSDVKTGNITQTQTGGSKAIQDMQIGNVKYSGESKVRTG
                     NITQTQTNASRSEQEMKVGNVQGAGKSDVKTGDILQSQQGEDSKQRMGIGNVTGSGKS
                     GVVTGPITQTQAGSKNTQGLQVGNVTGSGTSNVKIPGGITQTQSGEKNTQETNIGNVK
                     "
     gene            7122..>7427
                     /locus_tag="VFV55_10115"
     CDS             7122..>7427
                     /locus_tag="VFV55_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944697.1"
                     /translation="MRTHCLFVALAACALATLPAVVKAAGTEMLDLDPIMAAKVAKEK
                     AKAGAAGKGTGLSADKTTTAGKGECGVNIGNLDSGKQGPGKGFATPRDSTVVITGPVI
                     "
BASE COUNT         1229 a         2515 c         2647 g         1036 t
ORIGIN      
        1 ggctcgcggc cggccacggc caggagcgcg cggatgccgg cacacgattc gccggcccgg
       61 atgcgcgcca gcccctgcgc cacgaggatg cgattgttgg cgtcgagctt cacgacgtcg
      121 gccaccgtgc ccagggcgac caggtcgagg agggccgcca ggttcggttc cgcgcgcccc
      181 gcgaaggcgc cgcggcggcg caattccgcg cgcagcgcgg tcatcacgta gaagatcacg
      241 cccacgcccg cgaggttctt gctcgggaag ccgcagcccg cctggttggg gttcacgatg
      301 caggcggcgt ccggcctttc ctcgccgggg agatggtggt ccgtgacgag gacgcgcatg
      361 cccaggcggt tcgcctcggc cacgccctcg acggaggcga tgccgttgtc gacggtgacg
      421 agaaggccgg gcgagcgctc ggcggcgatg cgcacgatct ccggcgtgag cccgtagccg
      481 aaacggaaac ggtccggaac caggtagtcg accgtcgcgc ccatggcacg cagcgcacgc
      541 aggcccaccg cgcaggcggt cgccccgtcc gcatcgtagt cggcgacgat gagaatgcgg
      601 gttcctgcct cgatcgcatc ggcgagcagc gcagccgcct cggcggcgtg gccgaggcga
      661 tccgggaacg ccaggccggg cagggcggtg gagagctcgg cggcatcgga cacgccgcgc
      721 gcggcgaaca ggcgcgccag gcgcggatcc acgccgccgg cgacgagccg cgcgcgcgcg
      781 ctttcgtcga tcggtcgctc gacgacttca ggcatggctc acgagcgggc ggcggcggcg
      841 caggagccgc cagcgcgcgc ggcgtgcgat ctcgaatacc gcggtgtcgc gctcggacgg
      901 caggacgagg cggacgcttc cgaaagcggc cagggcctcg ccgagattcg agaaccagtc
      961 gtgctcgagg atgcgcgccg ctgcaagcca cgcctccatg tcgccgcggc ggaaaggcgt
     1021 gcggagggac tccaccacga cgaggcattg cgcggattcg cgcgagggca ggcgcgcggc
     1081 cccggccggc agcgactgga ccgcgcagcc gctcgcgagg gcgaggcttc gcgccagcgc
     1141 gtcggtcgct gccacgcacg catagggccg cggaagatcc gccggatagt gaccgccgcc
     1201 ccagaaccac accgcgttga gggtgggacg gccctccgat tcccggaccg cgttcaacgg
     1261 cagccgggcg agaagcatct gcgcctcgct gaagatcgac ggccacttca ggcgcacgcc
     1321 gccttccgga atcatgccga agggattgcg ccccacggcc tcctcgagcg gaacggtccg
     1381 tggcagttcc tccggcggca cgcgcaggta ccagcgctcg gcggacggcg cgacgaattc
     1441 gagcccgtcg cccgcgaaga acgcgttgag ttcgccgacg gccgcgtcgg cctcctcctg
     1501 cgtgagcgcg agcacgctcg catcgtgcag gacgagggcg ttgcgatcga cgcgctggtg
     1561 cacgggatcg gcgcgcaacc atgcgccttc gcggggtccc ccttcggcgg ccagcgtgag
     1621 cgcggccacc ggcgcctgca cgccaaggcc ccactggcga agaagccacg cgctgcccgt
     1681 tcccggctcg cgtgtcgcgt cggcgcgcgc gagccatgcc tccagcccgg gcaggcgaag
     1741 ggagcgcatc ggctcggggg cgtcggcggg ggcgagcagg tcggggacga ggagggtgag
     1801 cggcatggcg ggcaaccgtg gataataaac ggggttcgct cccctttctt ccttcccggt
     1861 cggttttctt gcagcctttc gagctcttcg tcggccttcg ctatacccgc gccaagcgcc
     1921 gcaaccattt catctcgttc atctcgctcg tctcgatgct ggggatcgcc ctgggcatcg
     1981 cggccctcat cacggtgatg tcggtgatga acgggttcga gcgcgagatc cgcgcccgca
     2041 tcctcggcgt ggccgcccac ctgcaggtgc tggcggtcga gggcgggctc gcggactggc
     2101 gcaaggtcgc cgcgcaggcg aaggccaatc cggaagtgct ggcggccgcg cccttcgtgg
     2161 cggcacaggg gctgctttcc tccgggtcgc aggtgcgcgg tgtcttcgtg cgcggggtgg
     2221 ttcccgaact cgaggagaag gtggccgatt tcggcgcgca catgcgcggc ggcaagctct
     2281 ccgacctgca gcccggcgag ttcggcatcg tactgggcgc cgagctcgcg cgggcgcttc
     2341 gattgcaggt gggcgagcgc gtgaccctca tcgcgccgca ggggcaggtc acgcccgcgg
     2401 gcctggtgcc gcgcctcaag cagtttcgcg tcgtcggcat tttctccgtc gggcacttcg
     2461 agtacgactc ggggctcgcg ctggtgcgca tggaggacgc gcaggtcctc taccgcctgg
     2521 gcgatgcggc cagcggcgtg cgcctgaagc tcgcgaacct ctacgaggcc ccgcgcgtgg
     2581 gccgcgagct ggcgaagacg atcgaggcgg acgcctacgt caccgactgg acgcagcaga
     2641 acgccaacta cttccgcgcc atccagatcg agaagcggat gatgttcatc atcctcacgc
     2701 tgatcatcgc ggtggcggcg ttcaatctcg tctcgacgct ggtgatggtg gtgaccgaca
     2761 agcaccccga catcgccatc ctgcgcaccc tcggggcgtc gccggcctcg atcatgaaga
     2821 tcttcatggt gcagggcacg ctcatcggcg tcatcggcac gggtctcggc gtgctgctcg
     2881 gggtgctcct gtcgctcaac atcgatgtcg tcgtgccggc ggtcgagcgc gccttcaact
     2941 tccagatcct ctcgcgcgag gtgtactaca tcagcgatct gccttcgcac ctcgagtggc
     3001 gcgacgtgtg gtcggtggcg gtcgtggcgc tgggcctcgc cttcatggcc acgctctatc
     3061 cctcctggcg tgcggcgcgc gtgaacccgg ccgaggccct gcgctatgag tgaggccgtc
     3121 ctttcctgcg ccgggcttgc caagaccttc gggctgggcg atgtcgccgt gcccgtgctg
     3181 agggcaatcg acttcgaggt cggcgccggc gaatcggtgg ccatcgtcgg cgcctcgggc
     3241 tcgggcaaga gcacgctcct gcacctgctc ggtggactgg accggccgag cgcgggcacg
     3301 gtgcgcatgg cgggcgagga catggcctcg ctcgatcccc gccgccaggg agagctgcgc
     3361 aaccgttgcc tgggtttcgt ctaccagttc caccacctgc tgccggagtt cacggcgctg
     3421 gaaaacgtgg caatgccgct cgccatccgc cggctcggcg cagcggaagc gaaggcgcgc
     3481 gcggccacca tgctgggcga ggtgggactc gggcaccggc tggcgcaccg gcccggcgag
     3541 ctttccggcg gcgagcgcca gcgcgtggcc ctggcgcgcg cgctggtcac ccagccggcc
     3601 tgcgtgctgg ccgacgagcc cacgggcaac ctggatcgcg gaacggcggg ccaggttttc
     3661 gacctcatcg tggaactcaa tgcccgtcac ggcacggcat tcgtgatggt gacgcacgat
     3721 ccggagctcg ccgcgcgcat ggaccggcgc ctgcggctgg ccgatgggcg gcttgcgcag
     3781 gactgaagcg ctagggacgg tccccgattg cgggttcggc acgcgccgcg atgtcgatca
     3841 accagcgctg cacgacctcg ccgcgcgcct cgacgaacgc ttccgattcg agccggccac
     3901 cgttgcccgc gatgacggcg gaagaagcga cgttgtcctt cgagcacgta agcagcacgt
     3961 ggtcgatgcc cagccgcgcc gcctgcgcga gcgtgagccg caggatcgca ttcccgagac
     4021 cctgaccccg cgcggtcggc cggatgccat agccgatgtg gccaccctcg acgcgaagcc
     4081 ggtcgttcag ccggtggcgc acgttggaca cgccgacgac ttcctcgccg cgcagcagcc
     4141 agaacgtgga atggggcacg aagccctcgg ggatcccgat gccttgctcg cagccggcaa
     4201 gctgggcgag aaaggcctcg aagttttcgt tggggaagga aagcgggaag gggatcagcc
     4261 gctcgccgcg ggcgaggaat tcggcgacga gggaccggta ggaatccttg tacgacgacg
     4321 atggcacgac gaggcgcggc gattccacgc tagtactcga ccggcaccgg gtcgagcgag
     4381 cgccacaggt accaggtcgc caccgagctc cacggctgcc acggcttcgc gatttcgcgc
     4441 atgtcgtccc gcgtgagcgc ctcgcccgag ttgtagaggc gctccatcgc gcgttgcagg
     4501 ccgaggtcgc ccaccggcag gacgttgggg cgcatgaggt ggaagatgag gaacatctcc
     4561 acgctccatc gcccgatgcc cttcacctgc accaggtcgg cgatgaccgc ctcgtcgtcc
     4621 atgcgcgtcc agcggcgggg ccgaagcgag ccttcgtgaa agcgcccggc gaggtccttg
     4681 aggtacgcga ccttcatgcg cgacaggccc gcggcgcgca tggcctcctc gtcgagtccg
     4741 acgatggcgg ccggcgcgag gttgcccgac agcgcctcga agcgctccca caccgcctgc
     4801 gccgcccgca ccgagatctg ctgcccgacg atggagcgcg ccagcgtccg gaaggcgtcg
     4861 ccgcgcgtgc gcaggttggc atccgggtgc gcgcgaatga tcccgcgcag caccttgtcc
     4921 ttcttcgcga ggtgcgtctt ggcctcgtcc cagtagcgcg gcttcatggc gtcgccctcg
     4981 cccggcgcgc gtgccgcgca tgccatgcgc gggtcacgag aatccgccac gcgaccatcg
     5041 tgagcgcata gcccccgatc gcgaacgcgg tgcactggat ggccagcccg atgagcagcg
     5101 tgtcgcccat cgagacgatc caggcgagca ggctcgggaa aaggagcacg agcccctccc
     5161 agttccagcc gatgtccggc ggcatgacga gcggcgccga gccgcccgtc acgagtccgc
     5221 cgatgttgta cgcgaggatg tagagcggca cgaaggtgaa ggggttggtg tagagcgtgg
     5281 tgaacatcgc ggccgggatg ttggcgcgca gcgggatcgc tatgagggcg gccaccagca
     5341 tctggaccgg accgggaatg aggccggtca cgaggccgat ggccacgccc agcgcgacgg
     5401 cgcggcggtg caggtgccag aggcgcgggt gcgtgagcac gggcgcgcac cattgcagcc
     5461 agcggtgccc gcggatgctt tccgcgctgg gcagccactt cctgagtttt cttcggatca
     5521 tgcggggtat ctgcgccgtt accgatgcaa tttggtggcc gcaagcgcgg ttgacttggg
     5581 tcaactgacc gcggggttgc gggaatagat tggacttggg tcattgtatt caaggtgcaa
     5641 catgaaagtc gcaacctgct tcatggccgt gctgctggtc gcaggcgccg ccgcaacaca
     5701 ggttttcgcg caggagggga ttccatcgct gcgcggcaag ccgacgaagg aagagatcgc
     5761 ccgtgcgctg tcgccgcccg cgccggagcc ggtgatgcgc tcccggggca tcgttctcaa
     5821 tcccggcgcc gggacggcgg ccgccaaggc agcgtcgacc aggcgcgcca tcgacctcga
     5881 ggtgctcttc gaattcgatt ccgcccgtct cacacccgac ggtcgcgacg tgctggactc
     5941 gctgggcggc gcgctgggct cgagcgagct cgccgacgtg cgccgcgtga ccctcgaggg
     6001 ccacaccgat gccaagggca gcgacgccta caacgattcg ctttcgctgc tgcgcgccca
     6061 gtcggcgcgc cagtacctca tccagaagca ccggatcacc ccggacaagc tgcacgcggt
     6121 cggcaagggc aaggcggaac ccgccgaccc gcgcgatccc tacggcgccg cgaaccgccg
     6181 cgttcgcgtc gtggtcgagg gccagtcctg aggtgccccg cagcgaaacc gccatgcgca
     6241 caccaaggag tacaccatgc cgaagaagaa tctccagctg atcgcggcac tcgtggccgc
     6301 cgttttcgcc gccggtgccc aggcccagca gacgggcagc aaggtagagg tcgacaaggt
     6361 cgagcagcgg cagaccggaa aggacaacaa gcagttcctc gatctcggca acgtcgagag
     6421 gggcggcaag tcggacgtca aggccggcac cgtcaagcag atgcaggggc agggacaggg
     6481 caacgtccag aagatgggcg tgggcaacgt cgccggcacc ggcagctcga aggtgaagac
     6541 cggcgacatc acccagaagc agatgggcgg ccagggcaac acgcagacga tgggcgtggg
     6601 caacgtcgcc ggcaccggaa agtccgacgt gaagaccggc aacatcacgc agacgcagac
     6661 cggcggctcg aaggccatcc aggacatgca gatcggcaac gtgaagtact cgggcgaatc
     6721 gaaggtgaga accggcaaca tcacccagac gcagacgaac gcctcgcgca gcgagcagga
     6781 gatgaaggtc ggcaacgtgc agggcgccgg caagtcggac gtgaagaccg gcgacatcct
     6841 ccagtcgcag cagggcgagg acagcaagca gaggatgggc atcggcaacg tcacgggcag
     6901 cggcaagagc ggcgtggtca ccggccccat cacccagacc caggcgggca gcaagaacac
     6961 gcagggcctg caagtgggca acgtgacggg aagcggaacc tccaacgtga agatcccggg
     7021 cggtatcacg cagacccaga gcggcgagaa gaacacccag gaaaccaaca tcggcaacgt
     7081 gaagtgaacc ggtaaggcaa ccggccggaa aggaggccag gatgcgcacc cattgcctgt
     7141 tcgtcgcgct tgctgcatgc gcgctcgcca cgctgcccgc cgtcgtgaag gccgcgggca
     7201 ccgagatgct cgatctcgac ccgatcatgg ccgccaaggt ggccaaggaa aaggccaagg
     7261 cgggcgcggc cgggaagggc accggccttt ccgccgacaa gaccaccacg gcgggcaagg
     7321 gcgagtgcgg ggtcaacatc ggcaatctcg actcgggcaa gcagggcccc ggcaagggct
     7381 tcgccacgcc ccgcgacagc acggtcgtca tcaccgggcc cgtgatc
//
LOCUS       DASUEV010000319         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1236916, whole genome shotgun sequence.
ACCESSION   DASUEV010000319 DASUEV010000000
VERSION     DASUEV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236916"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFV55_10120"
     CDS             <1..800
                     /locus_tag="VFV55_10120"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX4944698.1"
                     /translation="AAQEKGLKVFQFPSLKSGDATKAMKELEADIGIMAFVLQFAPQE
                     FVNIPKHGTIQYHPSLLPRYRGPSSINWPISRGEKKTGLTIFRPNDGLDEGPVILQKQ
                     TEVSENDTLGTVYFDRLFPMGVKAMLEAADLVVAGRHKEVVQDESQATYEGWFRANEA
                     KIHWHNHVDFIHDIIRGSDPAPGAWTTIGGKKLQLFGSRKHPVRTFGAVKGKIGEITE
                     IGAESMRVTAQGGQIEVTKVKPEEGKKVSAAEWAKAAGVGPGTILGG"
     gene            904..>2159
                     /locus_tag="VFV55_10125"
     CDS             904..>2159
                     /locus_tag="VFV55_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX4944699.1"
                     /translation="MEHRDVPGEARLRVGEAFLEDMGRGLVRLDAEDLRRLHAVPGDV
                     LQVTGRRSTVARAAEAPESHCGQLAAIIDGITRGNAQVGVDDWVTIRKVPFKPAGSLL
                     LGVVQAGQSPPAEDEIPHLRQLLSGVPVVPGDRLQVTFLGARPRSFVVEGAAPRGALL
                     VVPSTAIAFKASEVPGEKAYRVSYEDIGGLEREVALVREMVELPLKFPQLFGLLGIDP
                     PKGVLLTGPPGTGKTLIARAVSSEVRAHFVHVNGPEVIHKFYGESEAKLRQIFEEAQQ
                     NAPSIVFLDEFDALAPRRAKVIGDVEKRVVAQLLALMDGLVERGQVVVIGATNMPELI
                     DPALRRPGRFDREITIGVPDRTARARILAIHSRRMPLADDVDLDAIAEITHGYVGADL
                     EVLCKEAGMVALRRLVPQVHFEADLQP"
BASE COUNT          351 a          732 c          764 g          312 t
ORIGIN      
        1 tagccgcgca ggaaaagggg ctcaaggtgt tccagttccc ctcgctcaag agcggtgacg
       61 cgacgaaggc gatgaaggag ctcgaagccg acatcggcat catggccttc gtgctgcagt
      121 tcgcgccgca ggagttcgtg aacatcccga agcacgggac catccagtac cacccgtcgc
      181 tgctgcccag gtaccgcggg ccgtcgtcga tcaactggcc gatctcgcgc ggcgagaaga
      241 agacggggct cacgatcttc cgcccgaacg acggcctcga cgaggggccg gtgatcctgc
      301 agaagcagac cgaggtctcc gagaacgaca cgctcggcac cgtgtacttc gaccgcctgt
      361 tccccatggg cgtgaaggcg atgctcgagg cggccgacct cgtcgtggcc ggcaggcaca
      421 aggaagtcgt gcaggacgag tcgcaggcga cctacgaagg gtggtttcgc gccaacgagg
      481 cgaagatcca ctggcacaac cacgtggact tcatccacga catcatccgc gggtcggatc
      541 ccgcgcccgg cgcgtggacc acgatcggcg gcaagaagct gcagctcttc ggctcgcgca
      601 agcacccggt gcgcacgttc ggcgcggtga agggcaagat cggcgagatc accgagatcg
      661 gcgccgagtc gatgcgggtg accgcccagg gcgggcagat cgaagtgacc aaggtgaagc
      721 ccgaggaggg caagaaggtc tccgccgccg agtgggccaa ggccgccggc gtgggcccgg
      781 gcactatcct cggaggctga tccccgggtc ccggggcccg attccatccg gagtgacccg
      841 gatcagccct ctgcccccca tgcgccttgg gcgcaatatg ggagttctcg tgcggaggac
      901 acgatggaac accgggatgt cccgggcgaa gcccggctgc gggtgggcga agccttcctg
      961 gaagacatgg gccgcgggct cgtccgcctc gatgcggagg acctgaggcg cctgcacgcg
     1021 gtgcccggcg acgtgctgca ggtgaccggc cggcgctcca ccgtggcgcg cgccgccgag
     1081 gccccggaga gccactgcgg ccagcttgcg gcgatcatcg acggcatcac ccgcggaaac
     1141 gcgcaggtgg gcgtggacga ttgggtgacg atccgcaagg tgcccttcaa gcccgcgggc
     1201 tcgctgcttc tgggcgtggt gcaggcgggc cagtccccgc ctgccgagga cgagatcccg
     1261 caccttcgcc agctcctctc gggcgttccc gtggttccgg gcgaccgcct gcaggtcacg
     1321 ttcctcggtg cccggccgag gtccttcgtc gtcgagggcg cggcgccccg cggcgcgctt
     1381 ctcgtcgtcc cgagcaccgc gatcgccttc aaggcctcgg aagtcccggg cgagaaggcc
     1441 tatcgcgttt cctacgagga catcggcggg ctcgagaggg aagtcgcgct ggtgcgcgag
     1501 atggtcgagc tgccgctcaa gttcccgcaa ctcttcgggc tcctcggcat cgacccgccc
     1561 aagggcgtgc tcctcaccgg gccgcccggc accggcaaga cgcttatcgc gcgcgcggtg
     1621 tcgagcgagg tgcgcgcgca cttcgtgcac gtgaacggcc ccgaggtcat tcacaagttc
     1681 tacggcgaga gcgaggcgaa gctgcgccag atcttcgagg aggcgcagca gaacgcgccg
     1741 agcatcgtct tcctcgacga gttcgatgcg ctcgcgccgc ggcgcgcgaa ggtgatcggc
     1801 gacgtggaga agcgcgtggt cgcccagctc ctcgcgctca tggacgggct cgtggagcgc
     1861 ggccaggtgg tggtgatcgg ggcgaccaac atgccggagc tcatcgaccc ggcgctgcgc
     1921 aggcccgggc gcttcgatcg cgagatcacg atcggcgttc cggatcgcac ggcgcgcgcg
     1981 cggatcctcg ccatccactc gcgccgcatg ccgctcgccg acgacgtcga tctcgacgcg
     2041 atcgccgaga tcacgcacgg ctacgtcggc gcggacctcg aggtgctgtg caaggaggcg
     2101 ggcatggtgg cgctgcgccg cctcgtcccg caggtgcatt tcgaagccga cctccagcc
//
LOCUS       DASUEV010000320         6514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_656914, whole genome shotgun sequence.
ACCESSION   DASUEV010000320 DASUEV010000000
VERSION     DASUEV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656914"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /gene="holA"
                     /locus_tag="VFV55_10130"
     CDS             <1..1010
                     /gene="holA"
                     /locus_tag="VFV55_10130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX4944700.1"
                     /translation="TAPMKIATRQLAQHLKKGLSPLYAVHGAETLVALEAADAIRAAA
                     RREGCTEREVFTAEPGADWSKLGASAANLSLFASRKLIEIRIPTGKPGADGGKALEAL
                     CARLPEDAVVLVMLPELEWQQLKTAWFGALESAGTVVEARAISREELPQWLAERLARQ
                     QQQARVETLEWLADRVEGNLLAAKQEVEKLALLLPAGEISLDDIREAVTDVSRFERDG
                     LIDAIHAAEPGRIARAVDSLEAEGEPLPLLLWTVTEELRLMMQISGGERPRRFLDPDR
                     VQRIQRTARKHDAASFDRQLLRAHRIDRMIKGVETGDAWEEILELSLALAGHPALRSA
                     A"
     gene            1007..2326
                     /locus_tag="VFV55_10135"
     CDS             1007..2326
                     /locus_tag="VFV55_10135"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX4944701.1"
                     /translation="MRLPATKDPMNDLNPANDTQALIAALGLSARAASRVLARAGSAA
                     KNRALEVAAESLIAREKTILAANARDVKGARAEGADDAFIDRLKLTAKGIEEMAEGLR
                     QIAKLPDPVGEITDLRFRPSGIQVGHMRVPLGVVGIIYESRPDVTADAGGLCLKSGNA
                     AILRGGSEAMHSNKAIASCLHLGLKAADLPETAIQLVGTTDRAAVGELIAAEGYVDVI
                     VPRGGKSLIERISKDARVPVIKHLDGVCHVYVDAGADPAKALAIVDNAKTQRYGTCNT
                     METLLVAQSAAPSVLPAIGKMFAEKGVEMRACPESKAILERAGVRGVKDATEEDWYAE
                     YLAPIVAISTVPGIDEAIAHISKYGSQHTDAIVTGDWGRAQRFLREVDSASVMVNAST
                     RFADGFEYGLGAEIGISTNKLHARGPVGLEGLTSQKWIVLGDGHVRA"
     gene            2331..3008
                     /gene="nadD"
                     /locus_tag="VFV55_10140"
     CDS             2331..3008
                     /gene="nadD"
                     /locus_tag="VFV55_10140"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX4944702.1"
                     /translation="MASPLGLFGGTFDPVHFGHLRLAAEMREAFRLERVVFLPTGHPW
                     QRARDTIAGGAERVRMLELATAGEPAFTVDDREVRREGPTYSVETLAEYRNECGPDTP
                     LLFLCGTDAFARVETWHLWQSLFELAHFAVAVRADDPEWQSRGPGAIPRALWPRVTMS
                     LKDVLSTPAGRIMTFSMTPLAISSTAIRGLVQGRSSIRYLTPDPVVDFIQQHQLYGTQ
                     GLPAKLG"
     gene            3020..3547
                     /gene="rsfS"
                     /locus_tag="VFV55_10145"
     CDS             3020..3547
                     /gene="rsfS"
                     /locus_tag="VFV55_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX4944703.1"
                     /translation="MTRQLTTLAKKKLVVAALEDIKARDIQAIDVRKITSMFDWIVVA
                     SADSARQTKALARHVRDKLKEAGCAIIGTEGEEGAEWVLVDSGDIVAHIMQPAVRSYY
                     NLEELWGEGKFDRPANAATPLEKPLRREGKDKEKPAPRARRPKAAGAKPATRRKKAPA
                     GAPRAAKPRRRKPAA"
     gene            3548..4018
                     /gene="rlmH"
                     /locus_tag="VFV55_10150"
     CDS             3548..4018
                     /gene="rlmH"
                     /locus_tag="VFV55_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX4944704.1"
                     /translation="MRVTIVSVGHRMPAWIRSGFGEYVKRLPPEIKVGLVELKPEERT
                     SGKTLEKARALEGERIIAAIPDGATVYALDEKGRTVTTQGLSVMLAGWMRDAVHPAFV
                     IGGADGLSDEVRNRADKLVSLSALTLPHGLVRVVLAEQLYRAWSILANHPYHRE"
     gene            4011..4634
                     /locus_tag="VFV55_10155"
     CDS             4011..4634
                     /locus_tag="VFV55_10155"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX4944705.1"
                     /translation="MNSPITTIYLASRSPRRRELLAQIGVKFEVLLFREGTRQDAETI
                     EDPYPGEHPDDYVLRVTRMKVEAAWQRVVVRKGLRRMPVLAADTTVALANEMLAKPAD
                     AADAARMLRLLSGTRHRVLTAVAVAFAGRVEMRVSESHVTFCPLDESRIETYVASGEP
                     YDKAGGYGIQGRAGAFVTRLEGSYTGVMGLPLFETTELLREFGVAVP"
     gene            4840..6294
                     /gene="rng"
                     /locus_tag="VFV55_10160"
     CDS             4840..6294
                     /gene="rng"
                     /locus_tag="VFV55_10160"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEX4944706.1"
                     /translation="MNEQILINVTPQETRVAVTEQGAVQELHVERNSSRGIVGNIYLG
                     RVCRVLPGMQSAFVEIGLARAAFLHVADIWMARNGDGQFKPIEKILGEGQALLVQVVK
                     DPIGSKGARLSTQVSIAGRLLVYLPQDPHIGISQRIEDVAERENLREKVHTLIPEGES
                     GGFIVRTVAETATDAELKADIDYLVTLWKQIQQAATTAPPQAVLYQDLALSARVLRDL
                     VTDTTERVIVDSRETFHRLKDFAEKYTRKALPVLEHYTGERPLFDLFGVEEEIEKALA
                     RRVDLKSGGYVIIDQTEALTTVDVNTGGFVSGRSFDDTIFKTNLEAAQSIARQLRLRN
                     LGGIVIVDFIDMDTQEHRDAVMAEFRKALSRDRTRVTVNGFSQLGLVEMTRKRTRESL
                     AHVLCEPCPVCAGRGELKTAQTICYEILREILRTERQFGAKEYRILASQLVIDMFLDE
                     EANALTMLGDFISKPISMQVETAYTQEQYDVILV"
BASE COUNT         1106 a         2294 c         2166 g          948 t
ORIGIN      
        1 gcacagcccc gatgaagatc gccacccgac agctcgccca gcacctgaag aaggggctct
       61 cgccgttgta cgccgtgcac ggcgccgaaa cgctcgtcgc gctggaagcg gccgacgcca
      121 tccgcgccgc cgcgcgccgg gaaggctgca cggagcgcga agtcttcacc gccgagccgg
      181 gcgcggactg gtccaagctg ggcgcctcgg ccgccaacct gtcgctcttc gcctcgagga
      241 agctcatcga gatccgcatc cccacgggca agcccggcgc ggacggcggc aaggccctcg
      301 aagccctgtg cgcgcggctg ccggaggatg cggtggtgct cgtgatgctc ccggagctgg
      361 aatggcagca gctgaagacc gcctggttcg gcgcgctcga gagcgccggc accgtcgtgg
      421 aagccagggc catctcgcgc gaggaactgc cgcagtggct ggccgagcgc ctggccaggc
      481 agcagcagca ggcccgcgtc gagacgctcg agtggctggc cgatcgcgtg gagggcaacc
      541 tcctcgcggc gaagcaggag gtcgagaagc tggccctgct cctgccggcc ggcgagatct
      601 ccctcgacga catccgcgag gcggtgaccg acgtctcgcg cttcgagcgc gacgggctca
      661 tcgacgcgat ccacgcggcc gagcccgggc gcatcgcgcg cgccgtcgat tcgctggaag
      721 ccgagggcga gcccctgccg ctgctcctgt ggacggttac cgaggagctg cgcctcatga
      781 tgcagatctc gggaggggag cggccacgcc gcttcctcga cccggatcgc gtgcagcgca
      841 tccagcgcac ggcgcgcaag cacgacgccg cctccttcga ccggcaactc ttgcgggcac
      901 accggattga ccgcatgatc aaaggcgtcg agaccggcga cgcgtgggag gaaatcctcg
      961 agctttccct ggccctcgcc ggccatccgg cgctgcggtc cgccgcctga ggctccccgc
     1021 gacgaaagac ccgatgaacg acctgaaccc cgccaacgac acgcaggccc tcattgccgc
     1081 gctgggcctt tccgcccgcg ctgcgagccg ggtgctggcg cgcgccggca gcgccgccaa
     1141 gaaccgcgcc ctggaggtcg ctgccgagtc cctcatcgcc cgcgagaaga cgatcctcgc
     1201 cgcgaatgcg cgggacgtga agggcgcgag agcggaaggc gccgacgacg ccttcatcga
     1261 ccgcctcaag ctcaccgcga aggggatcga ggaaatggcc gaggggttgc gccagatcgc
     1321 aaaactgccc gatcccgtgg gcgagatcac cgacctgcgc ttccgtccct ccggcatcca
     1381 ggtggggcac atgcgcgtgc cgctgggcgt ggtcggcatc atctacgagt cgcgccccga
     1441 cgtgacggcg gacgcgggcg ggctgtgcct gaagagcggc aacgcggcca tcctgcgcgg
     1501 cggcagcgaa gccatgcatt ccaacaaggc cattgcctcc tgcctgcacc tgggactcaa
     1561 ggccgcggac ctcccggaga cggcgatcca gctcgtggga accaccgacc gcgcggccgt
     1621 cggcgagctc atcgccgccg aaggatacgt cgatgtcatc gtgccgcgcg ggggaaagag
     1681 cctcatcgag cgcatctcga aggatgcgcg cgtcccggtc atcaagcacc tcgacggcgt
     1741 gtgccacgtc tacgtcgacg cgggcgccga cccggccaag gcgctcgcca tcgtcgacaa
     1801 cgcgaagacc cagcgctacg ggacgtgcaa cacgatggaa accctcctcg tcgcgcaatc
     1861 cgcggcgccc tcggtgctgc ccgcgatcgg gaagatgttc gcggaaaagg gggtggagat
     1921 gcgcgcctgc cccgagtcga aggcgatcct ggagcgcgcg ggcgtgcgcg gcgtgaagga
     1981 cgccaccgag gaggactggt atgccgagta cctcgcgccc atcgtggcca tctcgaccgt
     2041 gcccggcatc gacgaggcca tcgcgcacat ttccaagtac ggatcgcaac acacggacgc
     2101 gatcgtcacc ggggactggg ggcgcgccca gcggttcctg cgcgaggtcg attccgcctc
     2161 ggtcatggtg aacgcctcga cacgcttcgc cgacggcttc gaatacggcc tgggcgccga
     2221 gatcggcatc tccaccaaca agctccacgc ccgcggcccc gtcgggctcg aggggcttac
     2281 gtcgcagaag tggatcgtgc tcggcgacgg gcacgttcgc gcctgagctt ttggcctccc
     2341 ccctcggcct ttttggcggc accttcgacc cggtccactt cggccacctg cggctcgcgg
     2401 cggaaatgcg cgaggccttc cgcctggagc gcgtcgtgtt cctgccgacc gggcatccgt
     2461 ggcagcgcgc ccgcgacacc attgcgggcg gggccgagcg cgtgcgcatg ctcgagctcg
     2521 ccaccgcggg tgaacccgca ttcaccgtgg acgaccgcga ggtgcgccgc gaggggccca
     2581 cgtacagcgt cgagacactc gcggaatacc gcaacgaatg cggccccgac acgccgcttc
     2641 tcttcctgtg cggcaccgac gccttcgcgc gcgtggagac ctggcacctc tggcaatcgc
     2701 tgttcgagct ggcgcacttc gccgtggcgg tgcgcgccga cgacccggag tggcagtccc
     2761 ggggacccgg ggcgattccc agggccctct ggccgcgcgt gacgatgtcc ctgaaggacg
     2821 tcctatccac gcccgcgggg cgcatcatga ccttctcgat gacgccgctc gccatctcct
     2881 ccaccgccat ccgcggcctc gtgcaggggc gcagctcgat ccgctacctc acccccgacc
     2941 ccgtggtcga cttcatccaa cagcaccagc tctacggaac ccaagggttg ccggcgaagc
     3001 tgggataatc aacgaaaata tgacccgcca actcactacg cttgccaaga agaagctcgt
     3061 cgtcgccgcc ctcgaggaca tcaaggcccg cgacatccag gccatcgacg tcaggaagat
     3121 cacctccatg ttcgactgga tcgtggtcgc gagcgcggat tccgcccgcc agaccaaggc
     3181 cctcgcccgc cacgtgcgcg acaagctgaa ggaggccggc tgcgccatca tcggcaccga
     3241 gggggaggaa ggcgccgaat gggtgctggt cgattccggc gacatcgtgg cgcacatcat
     3301 gcagcccgcg gtgcgcagct actacaacct cgaggagttg tggggcgagg gcaagttcga
     3361 ccgcccggcc aacgccgcca cgccgctcga gaagcccctg cgccgcgagg ggaaggacaa
     3421 ggaaaaaccc gcgccccgcg cgcgccgtcc caaggcggct ggcgcgaagc ccgcgacccg
     3481 caggaagaag gcgcccgccg gcgccccgcg cgcggccaag ccccgccgcc gcaagccggc
     3541 cgcctgaatg cgcgtcacga tcgtctcggt gggccacagg atgcccgcct ggatccgttc
     3601 cggcttcggc gagtacgtga agcgcctgcc gccggagatc aaggtcgggc tggtggagct
     3661 caagcccgag gagagaactt ccggcaagac cctcgagaag gcccgggcgc tcgaaggcga
     3721 gcgcatcatc gccgccatcc ccgatggggc gaccgtctac gcgctggacg aaaagggccg
     3781 caccgtcacg acgcaggggc tctccgtcat gctcgcgggg tggatgcgcg atgccgttca
     3841 cccggcgttc gtgatcggcg gcgccgacgg gctgtccgac gaggtgcgca accgcgccga
     3901 caagctcgtt tccctctcgg ccctcaccct tccgcacggg ctcgtgcgcg tcgtcctggc
     3961 ggagcagctc taccgcgcct ggtccatcct cgccaaccat ccgtaccacc gtgaatagcc
     4021 cgataaccac catttacctg gcctcccgca gcccgcgccg ccgcgagctt ctcgcccaga
     4081 tcggcgtgaa attcgaggtg ctcctctttc gcgagggcac gcggcaggac gcggagacca
     4141 tcgaggaccc ctatcccggc gagcatccgg acgactacgt tcttcgcgtg acgcggatga
     4201 aggtggaagc ggcatggcag cgcgtggtgg tccggaaggg actgcggcgc atgccggtgc
     4261 tcgcggccga cacaaccgtc gcgctcgcca acgagatgct cgccaagccc gcggacgccg
     4321 ccgacgcggc tcgcatgctg cggctgctct ccggaacgcg ccatcgcgtc ctgaccgcgg
     4381 tggcggtggc tttcgcgggg cgcgtggaga tgcgcgtgag cgagtcgcac gtgaccttct
     4441 gccccctcga cgagagccgg atcgaaacct acgtcgcctc cggcgagccc tacgacaagg
     4501 cgggcggcta cggcatccag gggcgcgccg gggccttcgt gacccgcctg gaggggagct
     4561 acacgggggt catggggctg ccgctcttcg agaccacgga gcttttgcgg gaattcgggg
     4621 tggcagtccc gtaagatgga accgaaaatc tttcgaacac ggatgaacac ggatgaacac
     4681 ggagatttca ggattggcat gagtcctatt tcctgactgg cggaagcggc agggcggttg
     4741 catcaggcac ggcgacccgt cccgagcctt atccgtgttc atccgtgtcc atccgtgttc
     4801 atccgtgttc caaaggtttg cgtgaaacct tgagccccga tgaacgagca gatactgatc
     4861 aacgtcaccc cgcaggaaac gcgggtcgcc gtcaccgagc agggcgcggt gcaggagctg
     4921 cacgtcgagc gcaactcctc gcgcggcatc gtcggcaaca tctacctcgg ccgcgtctgc
     4981 cgggtgctgc ccggcatgca gtcggcgttc gtggaaatcg ggctggcgcg cgccgccttc
     5041 ctgcacgtgg ccgacatctg gatggcgcgc aacggcgacg gccagttcaa gcccatcgag
     5101 aagatactcg gcgagggaca ggccctcctc gtgcaggtgg tgaaggaccc catcggctcg
     5161 aagggcgcgc ggctgtccac ccaggtctcc atcgccggcc ggctcctcgt ctacctgccg
     5221 caggacccgc acatcggcat ctcgcagcgc atcgaggacg tcgccgagcg cgagaacctg
     5281 cgcgagaagg tgcataccct cattcccgag ggcgaatccg gcgggttcat cgtgcgcacc
     5341 gtcgcggaaa cggcgaccga tgccgagctc aaggccgaca tcgactacct ggtcaccctg
     5401 tggaagcaga tccagcaggc ggccacgacc gcgcccccgc aggccgtgct ctaccaggac
     5461 ctcgccctgt ccgcgcgcgt gcttcgcgat ctcgtgaccg acaccacgga gcgcgtgatc
     5521 gtcgattcgc gcgagacctt ccaccgcctc aaggacttcg ccgagaagta cacgaggaag
     5581 gcgcttccgg tcctcgagca ctacacgggg gagcgcccgc tcttcgacct cttcggcgtc
     5641 gaggaggaga tagagaaggc cctggcgcgg cgcgtggacc tcaagagcgg cggctacgtc
     5701 atcatcgacc agacggaagc cctcaccacg gtggacgtga acaccggcgg cttcgtctcg
     5761 ggccgctcgt tcgacgacac catcttcaag accaacctcg aggccgcgca gtcgatcgcg
     5821 cgccagctgc gcctgcgcaa cctcggcggc attgtcatcg tcgatttcat cgacatggac
     5881 acccaggagc accgcgacgc ggtgatggcg gagttcagga aggccctgtc gcgcgaccgc
     5941 acgcgcgtca cggtgaacgg cttctcgcag ctcggcctgg tggagatgac gcgcaagagg
     6001 acgcgcgagt cgctggcgca cgtgctctgc gagccgtgcc cggtttgcgc ggggcgcggc
     6061 gagctcaaga ccgcccagac catctgctac gagatcctgc gcgagatcct gcgcaccgag
     6121 cggcagttcg gcgccaagga gtaccgaatc ctcgcctcgc agctggtgat cgacatgttc
     6181 ctcgacgagg aggcgaacgc cctcaccatg ctgggcgact tcatctccaa gcccatctcg
     6241 atgcaggtgg agaccgccta cacccaggag caatacgacg tgatcctcgt gtagccccgc
     6301 gaacgacggg ggcacggact ccttcagcgc gggccgccgt ggcgcatttg gcgcgagcca
     6361 ccactgtgtc gaaacgtgag gcaacgtcgg tatcagaacg cagttgtcac caccgtcgaa
     6421 gcgagaggct gggtcggggt ctggttggct gttctttgtt cttgttttcc ggccaggtca
     6481 aattgcttcc ggcgccacgt ctgcgaccta tgtc
//
LOCUS       DASUEV010000321         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_98110, whole genome shotgun sequence.
ACCESSION   DASUEV010000321 DASUEV010000000
VERSION     DASUEV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98110"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFV55_10165"
     CDS             <1..537
                     /locus_tag="VFV55_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HEX4944707.1"
                     /translation="LFQARGCSVLLAAGDTFRAAAREQLAAWGERHGVTVVAQDKGDS
                     AAVIFDAIQSARAKGIDVVLADTAGRLPTQLNLMEEIRKVRRVIGKADPTAPHETWLV
                     LDANTGQNAVAQVKAFDAALALTGLIVTKLDGTAKGGSLAAIAREHPVPILYIGIGEK
                     PDDLRPFVAADFVDALFE"
     gene            534..1193
                     /locus_tag="VFV55_10170"
     CDS             534..1193
                     /locus_tag="VFV55_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4944708.1"
                     /translation="MIQFSGVAKRYPNGHEVLRDVSFEVGAGELVVVTGHSGAGKSTL
                     LKLASGIERATRGSVLVNGQNLSSLGETALAILRRHIGFVFQDHKLLFDRPAWDNVAL
                     PLGIAGANADEAGRRVRASLDKVGLLGKEKAMPITLSGGEQQRLCIARAIVNRPAILV
                     ADEPTGNLDTESSREILDLFLSFNRVGVTVLLSTHDETSLALLGGRRLAIQGGQLASV
                     R"
     gene            1190..2101
                     /locus_tag="VFV55_10175"
     CDS             1190..2101
                     /locus_tag="VFV55_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944709.1"
                     /translation="MSTWLRLHRLALADAARRMAAQPVASTLAVAVMALAVALPLLAS
                     ILVGSVSRATSGFDTEPHVNVFLALDATDEDVRRIEAALKANPETASVRFVPRAKALE
                     ELKATSHLAELLANLDRNPLPHAFSVRTRTSDAARLGTARTEWAKLAKVDQVSAEFEW
                     AERISRWTRFAERALAGFAVVLAAAVLFIVGHLIRLQVLTMRDEIEVSQLIGATAADV
                     RRPFIYHGLLQGMLAGLAAVALAALVVAWLNAELQVLTSTYAYEFKIVFMDALGTVQV
                     IVAVAALGVIGAWLSVSRELRRFSAGV"
     gene            2168..3025
                     /gene="rpoH"
                     /locus_tag="VFV55_10180"
     CDS             2168..3025
                     /gene="rpoH"
                     /locus_tag="VFV55_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX4944710.1"
                     /translation="MTSATLPVLSLSPISSLEGYVQAVNAIPLLSVEREVELGRRLRE
                     KEDLSAARDLVLSHLRLVVSVARNYMGYGLPQADLIQEGNVGLMKAVRRFDPGRGVRL
                     ASFAIHWIKAEIHEYILRNWRLVKVATTKAQRKLFFNLRSLKHSTATLTPTEVRAMAK
                     TLDVKESDVVEMEQRMQGRDITIDPAPEDGEERVGPIAFLADASEGPAELVERHQNEI
                     LETEGLSRALASLDARSRRIVEARWLAEGESRTLHELADEFGVSAERIRQIEAKALQK
                     MKGSLAALR"
BASE COUNT          500 a         1062 c         1026 g          487 t
ORIGIN      
        1 ctgttccagg cccggggatg ctcggtgctg ctcgcggcgg gcgacacttt ccgcgccgcg
       61 gcgcgcgagc agctcgccgc ctggggcgag cgccacggcg tgaccgtcgt cgcgcaggac
      121 aagggcgatt cggccgccgt catcttcgat gccatccagt cggcccgcgc gaagggcatc
      181 gacgtggtcc ttgccgacac cgccgggcgc ctgccgacgc aactgaacct catggaggag
      241 atccgcaagg tccggcgcgt catcggcaag gccgacccca cggcgcccca cgagacctgg
      301 ctcgtcctgg acgccaacac ggggcagaac gcagtggcgc aggtaaaggc cttcgacgcc
      361 gccctcgcgc tcaccggact catcgtgacc aagctcgacg gcacggcgaa gggcggctcg
      421 ctcgccgcca tcgcgcgcga gcatccggtg ccgatcctct acatcggcat cggcgagaag
      481 cccgacgacc tgcgcccgtt cgtggcggcg gatttcgtgg acgcgctctt cgaatgatcc
      541 agttttccgg cgttgccaag cgctacccca acggtcacga ggtgcttcgc gacgtctcct
      601 tcgaggtggg cgcgggcgag ctcgtcgtgg tgaccgggca ctcgggcgcg ggcaagtcga
      661 ccctcctgaa gctcgcctcc ggcatcgagc gagccacgcg aggcagcgtc ctggtcaacg
      721 gccagaacct ctcctcgctc ggcgaaacgg cgctcgccat cctgcgtcgc cacatcgggt
      781 tcgtcttcca ggaccacaag ctgctcttcg accggccggc ctgggacaac gtggcccttc
      841 cgctcggcat cgccggcgcg aacgccgacg aggcgggccg ccgcgtgcgc gcgtccctcg
      901 acaaggtggg cctcctcggc aaggagaagg ccatgccgat caccctttcc ggcggcgagc
      961 agcagcggct gtgcatcgcg cgcgcgatcg tcaaccggcc cgccatcctc gtggccgacg
     1021 agccgacggg gaacctcgac accgagtcct cgcgcgagat cctcgacctg ttcctttcgt
     1081 tcaaccgcgt gggcgtgacc gtgctcctgt cgactcacga cgagacttcg cttgcgctgc
     1141 tgggcgggcg gcggctggcg atccagggcg gccagctggc cagcgtccga tgagcacctg
     1201 gcttcgcctg caccggctgg cgctggccga tgccgcgcgc cggatggccg cccagccggt
     1261 tgcgagcacg ctcgcggtcg cggtgatggc gctggccgtc gcccttcccc tgctcgcctc
     1321 gatactcgtg ggaagcgtat cgcgtgcgac ctcgggattc gataccgaac cgcacgtcaa
     1381 cgtgttcctc gcgctcgacg ccaccgacga ggacgtgcgc cgcatcgagg ccgcgctcaa
     1441 ggccaacccg gagacggcct ccgttcgctt cgtgccgcgc gccaaggcac tcgaggagct
     1501 caaggccacc tcgcacctgg cggaactgct tgcgaacctc gatcgcaacc cgctgccgca
     1561 tgccttttcc gtgcgcacca ggacgagcga cgctgcgcgg ctcggcaccg cccgaaccga
     1621 gtgggcgaag ctcgccaagg tcgaccaggt ttccgccgaa ttcgagtggg cggagaggat
     1681 ctcgcgctgg acgcgattcg ccgagcgcgc gctggccggc ttcgccgtgg tgctcgccgc
     1741 agccgtgctc ttcatcgtcg ggcacctcat ccggctgcag gtcctgacca tgcgcgacga
     1801 gatcgaggtg agccagctca tcggcgccac cgcggcggac gtgcggcgcc cgttcatcta
     1861 ccacggcctg ctccagggaa tgctcgcggg gctggcggcg gtcgcgctgg ctgccctcgt
     1921 ggtagcctgg ctgaatgccg aactgcaagt ccttacttcg acctatgcct atgaattcaa
     1981 gattgttttc atggatgcat tggggaccgt gcaggtcatt gtggcggtcg ccgccctcgg
     2041 ggtgatcggg gcctggctgt ccgtttcgcg cgagttgagg cgcttctcgg ccggggtctg
     2101 aggaacccgg cgggggatta gcactcaaat atcgcgagtg ctaaaatgtc cctaggagga
     2161 tgaccgcatg acgagcgcga ccttgcccgt actttcactt tcaccgatct ccagcctgga
     2221 ggggtatgtc caggccgtaa acgcgatccc gctcctcagc gtggaacgcg aggtggaact
     2281 gggccgccgc ctgcgcgaga aggaagacct gtcggccgcc cgcgacctgg tgctttcgca
     2341 cctgcggctg gtggtttccg tcgcacgcaa ctacatgggc tacggcctgc cgcaggccga
     2401 cctgatccag gaaggcaacg tcggcctgat gaaggcggtg cgccggttcg atccgggccg
     2461 tggcgtgcgc ctggcctctt tcgccatcca ctggatcaag gccgaaatcc acgagtacat
     2521 cctgcgcaac tggcggctgg tgaaggtggc cacgaccaag gcgcagcgca agctcttctt
     2581 caacctgcgc agcctcaagc actcgaccgc cacgctcacc ccgacggaag tccgcgcgat
     2641 ggcgaagacc ctcgacgtga aggaaagcga cgtggtcgag atggagcagc gcatgcaggg
     2701 tcgcgacatc acgatcgatc ccgcgccgga agacggcgag gagcgcgtcg gacccatcgc
     2761 cttcctggcg gacgcctccg aaggcccggc cgaactggtc gagcgccacc agaacgagat
     2821 tctcgagacc gagggacttt cccgtgcgct cgcgtccctg gacgcacgca gccggcgaat
     2881 cgtcgaggcg cgctggctcg ccgagggcga gtccaggacg ctgcacgagc tcgccgacga
     2941 gttcggcgtc tccgcagagc gcatccgcca gatcgaggcg aaggcgctgc agaaaatgaa
     3001 ggggagcctc gccgcgttgc gctgaaggaa aaggggtcag gttactttgc acaaaaaata
     3061 ggcaaaacgg aaaag
//
LOCUS       DASUEV010000322         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_98920, whole genome shotgun sequence.
ACCESSION   DASUEV010000322 DASUEV010000000
VERSION     DASUEV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98920"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFV55_10185"
     CDS             complement(<1..200)
                     /locus_tag="VFV55_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HEX4944711.1"
                     /translation="MPPNDPQWGKRPSDGPPDLDEILKKLNQKLAGLFARRPMDPSGP
                     TPPSGGPGFAFFGGSLAFIVLLV"
     gene            complement(284..1384)
                     /gene="hflX"
                     /locus_tag="VFV55_10190"
     CDS             complement(284..1384)
                     /gene="hflX"
                     /locus_tag="VFV55_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX4944712.1"
                     /translation="MCLEIGKRGAPDRAEEATSLVASAGGEVCEILNGRRERPDAATY
                     AGSGKVEELQSLCRLHDARSVVFDHSLTAVQVRNLETALSEGGKSVRVYDRTDLILEI
                     FAQRARSHDGKLQVELARLEHLSTRLVRGWTHLERQRGGLSKTGGPGEKQIELDRRLI
                     GERVKKLKERLKKVARTRATQRAARTRSGMLRVSIVGYTNAGKSTIFNRLTRADSYVA
                     DKLFATLDPTTRRLHLGDGVNAALSDTVGFVRDLPHGLVAAFRSTLEETVQADVLLHV
                     VDASSPRHDEQIDAVNVVLAEIGAADIPQIVVWNKIDRIPGAVSEVVRDPCGKILNIK
                     VSAVSGAGIEELRAALAEVARVNDPRILAPAA"
     gene            complement(1419..1658)
                     /gene="hfq"
                     /locus_tag="VFV55_10195"
     CDS             complement(1419..1658)
                     /gene="hfq"
                     /locus_tag="VFV55_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX4944713.1"
                     /translation="MSNKGQLLQDPFLNILRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVSVPAEHSPES"
     gene            complement(1928..3328)
                     /gene="der"
                     /locus_tag="VFV55_10200"
     CDS             complement(1928..3328)
                     /gene="der"
                     /locus_tag="VFV55_10200"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX4944714.1"
                     /translation="MKPTVVLVGRPNVGKSTLFNRITRTRDALVHDMPGLTRDRHYGR
                     GRMGDKPFIVVDTGGFEPVAKDGILSEMAHQAQEAIAEADVVIFVVDGRAGLAPQDKR
                     IAKLLRERGAPVLLAVNKTEGMQQDIAVSEFHELALGEPHAISAAHGEGVRQLVEDAL
                     APFPVEPEDSDVEPDHPKVAIVGRPNVGKSTLVNRVLGEERMIAFDEPGTTRDAIEIE
                     ARWQGRRYTIVDTAGIRRKGKVFESVEKFSVVKTLQAIEQANVVVLVVDAAQDISEQD
                     AHIASYILEAGRALAVAVNKWDAIDAGKRKDAKTDLERKLAFLDFAEFVPISALKGTG
                     IKTLFEAVDGAFAAAMAKLSTPKLTRALQAAVEKQAPPLAGKMRPKLRYAHQGGSNPP
                     VVVIHGTSVAAIKEGYRRYLENTFVKVFKLKGTPLRVELRQGANPYQDKKRSILTPRQ
                     EAKKRREKRYQKKTFG"
BASE COUNT          531 a         1082 c         1198 g          644 t
ORIGIN      
        1 acgagcagga cgatgaaggc gagcgagccc ccaaagaagg cgaagccggg accgcccgac
       61 ggcggcgtcg ggccggatgg atccatgggc cggcgcgcga agagtcccgc gagcttctgg
      121 ttgagcttct tcaggatctc gtcgaggtcc gggggaccgt cgctgggacg ttttccccac
      181 tggggatcgt tgggaggcat gcggtggctg tctggcggga aatggggacg aggacgaggg
      241 gcgggccgct gggggccgat tcaatgcgcg acgggttgat agatcaggcg gccggggcca
      301 ggatgcgggg gtcgtttact cgggccactt cggccagcgc tgcgcgaagt tcctcgatgc
      361 ccgcgccgga aacggcactc accttgatat tcaagatctt accacaagga tcgcgcacga
      421 cttccgaaac ggccccggga atgcggtcga tcttgttcca gacgacgatt tgcgggatgt
      481 cggcggctcc gatttcggcc aggaccacgt tcaccgcgtc gatctgctcg tcgtggcggg
      541 ggctggaggc gtccaccacg tgcagcagga cgtcggcctg caccgtctcc tcgagcgtgg
      601 aacggaaggc cgccacgagg ccgtgcggca ggtcgcgcac gaagcccacg gtgtcggaca
      661 aggcggcatt cacgccgtcg cccaggtgca ggcggcgcgt ggtgggatcg agggtcgcga
      721 aaagcttgtc ggccacgtac gagtcggcgc gcgtaaggcg gttgaagatc gtcgacttgc
      781 cggcgttggt gtagcccacg atcgacacac gcagcatgcc ggagcgggtg cgggcggcac
      841 gctgcgtggc gcgcgtgcgg gccaccttct tcaggcgctc cttcaatttc ttcacgcgct
      901 cgccgatgag gcggcggtcc agctcgatct gcttctcgcc cgggccgccg gtcttcgaca
      961 ggccgccgcg ctggcgctcg aggtgggtcc agccgcgcac caggcgcgtg gagaggtgct
     1021 ccaggcgcgc gagctcgacc tgcagcttgc cgtcgtggct gcgggcgcgc tgggcgaaga
     1081 tctcgaggat caggtcggtg cggtcgtaga cacgcacgct cttgccgccc tcggagagag
     1141 ccgtctcgag gttgcggacc tggacggcgg tcagcgaatg gtcgaagacg accgaacgcg
     1201 cgtcgtgcag ccggcagagg ctctgcagtt cctcgacctt gccggagcct gcataggtgg
     1261 ccgcatcggg ccgctcgcgt cgtccgttga ggatttcgca gacttcgccg cctgcgctgg
     1321 cgaccaggct ggtggcttcc tcggcacgat cgggcgcgcc tcgcttgccg atttccaggc
     1381 acacgaggat ggttcgctcc cgtcgcgaaa ccggggggtc aggattccgg ggaatgttca
     1441 gccgggaccg acaccgggcg ggccggtacc actgtggaaa tggcgtgctt gtagaccatc
     1501 tgcgtcacgg tgttcttgag gaggacgacg tattggtcga acgactcgat ctggccttgc
     1561 agcttgatgc cgttcacgag gtagatcgag accggaacgt gttccttgcg caggatgttc
     1621 aggaagggat cctgaagaag ttgtcccttg ttgctcattt tttacccctt ttagttacgg
     1681 gtttgttgcc gggccggtcc tgtcgctcgc aagggttgcc ggtcacggcc ttccacttgc
     1741 tcggagactc tactggaaag ccggaaattc cgccacccaa caaggacaac tccctgctcc
     1801 ccaagttccc ccggttacga ctaaaggggt caggttactt tgcacaaaaa ttgagcaggg
     1861 cgagactccg gcgcctgacc ctgctcaatt attgtgcaaa gtaacctgac ccctttagcc
     1921 cgccgggtca gccgaaggtc tttttctggt agcgcttctc gcggcgcttc ttcgcttcct
     1981 ggcgaggggt gaggatggag cgcttcttgt cctggtaggg gttggcgccc tggcgcagtt
     2041 cgacgcgcag tggcgtgccc ttgagcttga agaccttgac gaaggtgttc tccaggtagc
     2101 ggcggtagcc ttccttgatc gcggcgacgc tcgtgccgtg gatgaccacc accgggggat
     2161 tggagccgcc ctggtgggca tagcgcagct tcggccgcat cttgccggcg agcggcgggg
     2221 cctgcttctc gacggcggcc tgcagcgccc gcgtgagctt gggcgtggac agcttggcca
     2281 tcgccgcggc gaaggcgccg tcgaccgcct cgaaaagcgt cttgatcccc gtgcccttca
     2341 gcgccgaaat gggcacgaac tcggcgaaat cgaggaacgc gagctttctc tccaggtcgg
     2401 tcttcgcgtc cttgcgcttc ccggcgtcga tggcgtccca cttgttcacg gcgaccgcga
     2461 gcgcccggcc cgcctcgagg atgtagctgg cgatgtgcgc gtcctgctcg gagatgtcct
     2521 gcgcggcatc caccaccagc accacgacgt tcgcctgctc gatggcctgc agcgtcttca
     2581 ccaccgagaa cttctcgacc gactcgaaca ccttgccctt gcggcggatg ccggcggtgt
     2641 cgacgatcgt gtagcggcgg ccctgccagc gcgcctcgat ctcgatcgcg tcgcgcgtgg
     2701 tgcccggctc gtcgaaggcg atcatgcgct cctcgcccag cacgcggttc acgagcgtgg
     2761 atttgccgac gttcggccgc ccgacgatgg ccaccttggg gtggtcgggc tcgacgtcgg
     2821 aatcctcggg ctccacgggg aacggggcca gcgcgtcctc gacgagttgc cggacgccct
     2881 ccccgtgcgc cgcggagatc gcgtgcggct cgcccagcgc cagctcgtgg aactccgaga
     2941 cggcgatgtc ctgctgcatg ccctcggtct tgttgaccgc cagcagcacc ggcgcgccgc
     3001 gctcgcgcag gagcttggcg atgcgcttgt cctgcggagc gagcccggcg cggccgtcga
     3061 ccacgaagat gaccacgtcg gcctccgcga tggcctcctg cgcctggtgc gccatctcgg
     3121 acaggatgcc gtccttggcc accggctcga agccgccggt atcgaccacg atgaagggct
     3181 tgtcgcccat gcgcccgcgg ccgtagtggc ggtcgcgcgt gagaccgggc atgtcgtgca
     3241 ccagcgcatc acgcgttcgc gtgatccggt tgaagagcgt gctcttgccg acgttggggc
     3301 ggccgacgag aacgacagtg ggtttcatgg gcgcttgatt gatcagtgcg ggcaaaaggg
     3361 gtcaggtaaa agggggcagg ttacttcggt aaaaggggtc aggttacttt actgtcgact
     3421 gatttccgac gggctgtaaa gtaacctgac ccctt
//
LOCUS       DASUEV010000323        10933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1480142, whole genome shotgun sequence.
ACCESSION   DASUEV010000323 DASUEV010000000
VERSION     DASUEV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480142"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1452
                     /locus_tag="VFV55_10205"
     CDS             121..1452
                     /locus_tag="VFV55_10205"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4944715.1"
                     /translation="MERRTMATILQQIHPRRWVNGLWRNPDFVKLWGSLTIVHFGGHI
                     TFLALPLTAALMLNATPFEVGVLTALEALPYPLFGLFAGVLVDRASKLPVIIWADLGR
                     GLALASVPACAWLGVLSMPVLYLAGFLVGVGSVIGWPAYQVFMTERVGRENLVEANAK
                     IGISDSASQLVGPGLAGALIQWLTAPIAILLDALSFVASAWMLRGIPPAATDAPKISG
                     ESVWREIGEGLVAVWRNRTLRALAWSLAAWQVFRHAYVAVVILFAARELGFSAGHVGV
                     LFMAAGLGSLAAAGAVAPLNARYGFGPTMLVALGGTGIAWLVLGAASGDYLAASATFG
                     LGLFLLDFSAMTFFINYLTLRQTVTPDRLLGRVTATMICLTVATAPLGGLAGGWIAEH
                     WGLRATILLAGAGAILMIPLVAWTSPLARMRTLPTPQEPVVPESVAEEMAG"
     gene            1592..1957
                     /locus_tag="VFV55_10210"
     CDS             1592..1957
                     /locus_tag="VFV55_10210"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX4944716.1"
                     /translation="METSEVVVAMTGMLAGLGLPLLVIAIVLFYKHQRNRMRQETILK
                     LAEKGLPVPPELFRLPEHAPSPKGGLVLLALGIALSAFFWERGQPWSIGLIPGLMGVA
                     LLIAWKIDSRKADRREPPR"
     gene            1963..2511
                     /locus_tag="VFV55_10215"
     CDS             1963..2511
                     /locus_tag="VFV55_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX4944717.1"
                     /translation="MGERPEPADAELVLAVVAHDDREAFSRLVRRHQSMVRSVLRRLT
                     KGDHGLADDLAQETFLLAWRNLRSFRFDARFSTWLYRIAFNAWLSQARKRREVLLDDT
                     EAVPVNEEPAHAVPAAERIDLERALGELSDAERVAIATCYYADLTHEEAARAIGIPLG
                     TVKTLILRAKAKLRAQLGEDYP"
     gene            2508..2837
                     /locus_tag="VFV55_10220"
     CDS             2508..2837
                     /locus_tag="VFV55_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944718.1"
                     /translation="MTDPMHDRIEQFLREDARRHLADDGFSLRVASSLPPETATRAWL
                     KHALVLGSAAIGSVLAWLFAPAGTSLVQGFLDLARLHAHTPSAFAALGLALAMAVTAA
                     VLVAEEN"
     gene            complement(2846..3181)
                     /locus_tag="VFV55_10225"
     CDS             complement(2846..3181)
                     /locus_tag="VFV55_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX4944719.1"
                     /translation="MDRSTALKVFESLGSSLRLDLFCLLVRAGETGKVAGELADALEI
                     PATNLSFHLKAMLQASLVTVRQEGRFLRYRANVPHMLRVLAYVMENCANAEAGEGAGA
                     TAANRAPPR"
     gene            3311..5506
                     /locus_tag="VFV55_10230"
     CDS             3311..5506
                     /locus_tag="VFV55_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4944720.1"
                     /translation="MEQLSRRRFLKISAATFGAGAVGSQIPVVPSLAMGSKPPAGKVE
                     TIPTYCEMCFWKCGGIAHVRDGKLWKFEGNPLDPMSRGRLCPRGTGAPGTHYDPDRLR
                     KPLIRRGERGKEEWTAVTWDEALNHIAEKMKKIAAEHGPESIALWKHGAGSRFFEHVM
                     RAYGCVNKAAPSFAQCRGPRDIGYLLTYGTGLGSPEPTDIANTKCLVLIGTHLGENMH
                     NTQVQEFAQAIANKATIIVVDPRFSVAASKAKHWLPIRPGTDIALLLAWMNVLVTEGL
                     YDKEFVAKYGHGFDKFTAAIAANTPEWAEGETGIPAATIRATAREMASHRPAALVHPG
                     RRTNWNGDDTQRARSTALLNALLGNWGRKGGLYLSSGMTFAGYPFPKYPEAKKPKVDN
                     PDGRWPFADEEVTTGLREATITGKPYPIKAWVVYASNLLQTLPSEKETLEAISKLDLL
                     VVVDTHPSEISGYADVVLPDTMFLERHDDLYVGWGRQGWTALRQPVVAPPGEQKPAWW
                     IAKQLADRLGVGEFMPFKDMDEYLAARCAKNGIDYAKLRKDGILMGEKKPITIEEGAE
                     IAFDTPSKKVEFWSDQLAKAGFDPVPKYTAPEAAPAGFYRLVTGRAPVHTFSRTQTNV
                     YLRDLMKENEVWVNTATAAQAGLKHGQYVKLKNQDGVVSNRVKVKATQRIRPDTLYMV
                     YGFGHTNKMLKSAYLMGASAAQLNTRYKVDPLMGGTSIHQNFVAFEKEA"
     gene            5508..6047
                     /locus_tag="VFV55_10235"
     CDS             5508..6047
                     /locus_tag="VFV55_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX4944721.1"
                     /translation="MPRFGMVIDTLKCVGCMDCVVACQTENDVPQGHCRDWIVTELRG
                     EFPTLSLEIRSERCNHCDNPPCVSCCPTGASHVEDFGKTVQVTAEKCIGCKACLSACP
                     YGARFVHPDGYADKCTFCLHRVKEGKDPACVSVCPTRCMQFGDLDDPKSAVSRLLASR
                     KHHTLLPEAGTKPRVFYLA"
     gene            6058..7104
                     /gene="nrfD"
                     /locus_tag="VFV55_10240"
     CDS             6058..7104
                     /gene="nrfD"
                     /locus_tag="VFV55_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX4944722.1"
                     /translation="MLEITTTRHNPFVDPMLSVWSWEIPVYLFLGGMIAGMMVLGGIN
                     MLKLARGERPEGFYSVQTPVLGFVLLNLGMGALFLDLAHKLYVYRVYMAFEPTSPMSW
                     GSWVLILVFPVLIVSALIRLPEAWPWLGERVPAARGWSAALLARPGVIKALGYLNVFL
                     GIGLGIYTGILLSTMVARPLWNSAILGPLFLVSGLSAAAAMVHLVASFVPGHPAPRGL
                     VGGALALLVQPLGERPPEPGTATSLVRADLAFLAVELVLIGLLVIGLLSSSASHAAAA
                     QIILSGKFAVLFWAGVIAAGIVVPLLLQLLELGHRIPHTIVPALLVLAGGLALRWVMV
                     GAGQASHVIAATGL"
     gene            7118..7675
                     /locus_tag="VFV55_10245"
     CDS             7118..7675
                     /locus_tag="VFV55_10245"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX4944723.1"
                     /translation="MTTLAASSISSRAGAKSSAAPYWNPYAAGFGLGLVLLAAYLVAG
                     RGLGATGAFSSVAAWLANAISPEHAQASAVHARYLESGPPLTAWLTWLLIGTFIGAAV
                     SGLAAGRFAISVEKGPRVTDGQRLALALAGGIIAGLGAKIARGCTSGQALTGASILNP
                     GSLVFMLAVFVAGYAVAWTYRKEWL"
     gene            7675..8265
                     /locus_tag="VFV55_10250"
     CDS             7675..8265
                     /locus_tag="VFV55_10250"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX4944724.1"
                     /translation="MFPLSSTMEFTQVTTLALAAVIGIGFGFFLEKAGFGSARKLMAV
                     FYLYDMAVVKVMFTAIVTAMVGIFVLSTAGMLDLAELYIEPSNYPAAIIGGLVFGVGF
                     VIGGYCPGTSLAACATGRLDGFAFVAGIFAGSYAYAEFLSGFDTWLAQSAQPDLTLPL
                     LTGIPMGWFVLAFIAFLALAGWGMSAAERRFAGLRP"
     gene            8302..9630
                     /locus_tag="VFV55_10255"
     CDS             8302..9630
                     /locus_tag="VFV55_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX4944725.1"
                     /translation="MKIKRLYSALALAGLVVPSVALATDGYFSHAYGLKSLGMGGAAI
                     AVASEPFGGAVNPAAMTSLGNAWQAGVQVFMPRREAERSGSGLAGIDGSVSSDSLAFA
                     IPEFGFNYMARPDLAVGVSVVGNGGMNTDFRGEQIPAQSACAAFNPAPGPYNLLCGNG
                     RLGVDLMQLLVAPYAAWKVSPSHSIGAAPVLAYQRFRAQGLQAFDNPGLSTSPGDVTN
                     RGYASATGYGVRLGYHGTFDKFSVGVAWASKLSMGEFDKYKGLFAEQGVFDIPSNLTV
                     GLAWRPDNKWLVAVDWERIKYSDSKAVSNASGLLLNCFGGDRTSCMGGSNGPGFGWND
                     IDVWKFGFEYAVDAKLTLRAGYNRSENPINAADVTFNILAPGVIKDHYTVGATWKLDS
                     RSEITAYGLYASNNTVSGTSLFVPFGAPPTTSETIQLKEMTVGLGYTSHF"
     gene            complement(9743..10729)
                     /locus_tag="VFV55_10260"
     CDS             complement(9743..10729)
                     /locus_tag="VFV55_10260"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX4944726.1"
                     /translation="MNKPMRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEIP
                     DEKAQKALKGVMMELDDCAFPLLAGMSAASDAKVAFKDADVAILVGARPRGPGMERKD
                     LLEANGAIFTVQGRALDEGASRNVRVLVVGNPANTNAYIAMKSAKSLSPTCFTSMMRL
                     DHNRAASQLAAKTGKPVESFEKLVVWGNHSPTMYPDYRFATAGGQSVKALVNDEAWYK
                     DTFMPLVGKRGAAIIEARGLSSAASAANAAIDHVHDWVLGSGGKWVTMGVVSDGSYGI
                     PEGVMYGFPCTTANGKYQIVKGLEIDEFSRQRMDKTLAELEEERAAVKHLLG"
BASE COUNT         1661 a         3862 c         3554 g         1856 t
ORIGIN      
        1 cgaggcggga gggtcggtcg aaaaattgcc gtcacctttc cgcctcgcgc tcgagcgacg
       61 gcgactcgtc gcattggggt ctgaccccga tcttggggcc tgaccccgaa cttgaagacg
      121 atggaaagaa gaacaatggc aacgatcctg cagcagattc acccccgccg ttgggtcaac
      181 ggcctgtggc gcaacccgga cttcgtcaag ctctggggct cgctcacgat cgtgcacttc
      241 ggcggccaca tcacgttcct cgcgctgccg ctcacggcgg cgctgatgct gaacgccacc
      301 ccgttcgagg tgggcgtgct cacggcgctc gaggcgcttc cctaccccct cttcggcctc
      361 ttcgcgggcg tcctcgtgga tcgcgcgagc aagctcccgg tgatcatctg ggccgacctg
      421 ggccgcggcc tggcacttgc ctcggtgccc gcttgcgcgt ggctcggcgt gctctccatg
      481 ccggtgctct acctcgcggg cttcctcgtc ggcgtggggt cggtcatcgg ctggccggcc
      541 taccaggtgt tcatgaccga gcgtgtcggc cgcgagaacc tcgtcgaggc caacgcgaag
      601 atcgggatct ccgactccgc ctcgcaactc gtggggccgg ggctcgcggg tgcgctgatc
      661 cagtggctca cggctcccat cgccatcctg ctcgacgccc tttcgttcgt cgcgtccgcg
      721 tggatgctgc gcggcattcc gcccgcggcc acggacgcgc cgaagatctc gggcgagagc
      781 gtgtggcgcg agatcggcga ggggctcgtc gcggtgtggc gcaaccggac gctgcgggcg
      841 ctggcctggt cgctcgccgc ctggcaggtg ttccgccacg cctatgtcgc ggtggtgatc
      901 cttttcgcgg cgcgcgagct gggcttctcc gccggccacg tcggcgttct cttcatggcc
      961 gccggcctgg ggtcgctcgc ggccgccggc gccgtcgccc cgctcaacgc ccgctacggg
     1021 ttcgggccga ccatgctggt ggcgctgggc ggaacgggga tcgcctggct cgtgctcggc
     1081 gccgcctcgg gagactacct cgccgcgtcc gcgacgttcg ggctggggct cttcctgctc
     1141 gacttctcgg cgatgacttt cttcatcaac tacctgacgc ttcgccagac ggtgacgccc
     1201 gaccggctgc tcggccgcgt aacggcgacg atgatctgcc ttacggtggc cacggcgccc
     1261 ctggggggac tcgccggagg ctggatcgcc gagcactggg gactgcgggc gacgatcctg
     1321 ctggccggcg caggggcgat cctgatgatt cccctggtcg cctggacatc gccgctcgcg
     1381 cggatgagga cgttgccgac cccgcaggaa cccgttgtcc ccgaaagcgt ggcagaggag
     1441 atggccggct gaatgcgggg gcgggcgaat ctgccggccg ctcccgccgc atcctacggg
     1501 catggaaacc agccatccgc ctgaacgccg gtttgccgcg cgcccgggcc gaatcccata
     1561 atgcgggccg ttgcggccct cggagcaagc catggagacc tcggaagtcg tggtcgccat
     1621 gacgggcatg ctggctggcc ttggcctgcc actgctggtc atcgcgatcg ttctcttcta
     1681 caagcaccag cgaaaccgca tgcgccagga gacgatcctc aagctcgcgg aaaagggcct
     1741 gccagtgccg ccggaactct tccggctgcc cgagcacgcg ccgagcccga agggggggct
     1801 ggtgctcctc gcgctcggga tcgcgctttc cgccttcttc tgggaacggg gccagccgtg
     1861 gtcgatcggg ctcatccccg gtctgatggg ggtggcgctc ctcatcgcct ggaagatcga
     1921 ctcgcggaag gcggaccgcc gcgagccgcc gcgttaacgc cgatgggcga acggccggaa
     1981 ccggccgatg cggagctcgt cctcgcggtc gtcgcccatg acgaccgcga ggcgttttcg
     2041 cgcctggtgc gccgccacca gtcgatggtg cgttcggtgc tgcgccggct gaccaagggc
     2101 gaccacggcc tcgccgacga cctcgcgcag gaaacgtttc tcctcgcctg gcgaaacctg
     2161 cgctccttcc gcttcgacgc gcgcttctcg acctggctgt accggatcgc cttcaacgcc
     2221 tggctttcgc aggcgcgcaa gcggcgcgag gtccttctgg acgacacgga ggccgtgccc
     2281 gtcaatgagg aacccgcgca cgcggtaccg gccgccgagc gcatcgacct ggagcgcgca
     2341 ctcggcgaac tgtccgacgc ggagcgcgtc gccatcgcca cctgctacta cgccgatctg
     2401 acccacgagg aggccgccag ggcgatcggc atcccgctcg gcaccgtgaa gacccttatc
     2461 ctgcgcgcga aggcaaagct gcgcgcgcaa ctgggcgagg attacccatg accgacccaa
     2521 tgcacgaccg catcgaacaa ttcctgcgcg aggacgcgcg gcggcacctg gccgacgacg
     2581 gcttctcgct tcgcgtcgcg agttccctcc cgccggagac tgcgacacgg gcatggctca
     2641 agcacgccct ggtgctcggc tcggccgcga tcggcagcgt tctggcatgg ctgttcgcgc
     2701 ccgccgggac gtcgctcgtg cagggcttcc tcgacctcgc gcgccttcat gcgcatacgc
     2761 cttccgcgtt cgcggccctg ggcctggccc tggcgatggc cgtgacggca gcggtgctcg
     2821 tggccgagga gaactgaagc gcaggctagc gcgggggcgc gcggttcgct gccgtcgcgc
     2881 cagcgccttc gccggcctcg gcgttcgcgc aattctccat cacgtaggcg agcacgcgca
     2941 gcatgtgcgg aacgttggcc cggtagcgca ggaaccgccc ctcctggcgc acggtgacca
     3001 gactcgcctg cagcatcgcc ttgaggtgga aggacaggtt ggttgcggga atttcgagcg
     3061 cgtcggcgag ctcgccggcc accttccccg tctcgcccgc gcggaccagc aggcagaaaa
     3121 ggtccagccg caggctggag cccagcgact cgaacacttt cagcgccgtt gaacggtcca
     3181 tccccatctc ctcatcgggg cacggttcga atctactcgg gaaatcgggg cgcgacttga
     3241 tgtatatcag ttgctactaa tgcaaatagg attgaaatga tgccatcccc tccccacaac
     3301 cgaggacgag atggagcagc tctcccgcag gcgttttctc aagatcagtg ccgccacctt
     3361 cggggccggc gcggtcggtt cccagatccc ggtcgtcccg tcgctcgcca tggggtcgaa
     3421 gcccccggcg ggcaaggtcg agacgattcc gacctactgc gaaatgtgct tctggaaatg
     3481 cggcggcatc gcgcacgtcc gtgacggcaa gctgtggaaa ttcgagggca acccgctcga
     3541 cccgatgagc cgtggccgcc tgtgcccgcg cggcaccggc gcacccggca cccattacga
     3601 tcccgaccgc ctgcgcaagc ccctgatccg gcggggagag cgcggcaagg aagagtggac
     3661 cgcggtcacc tgggacgagg ccctgaacca catcgcggag aagatgaaga agatcgccgc
     3721 cgagcacggc cccgagtcga tcgcgctgtg gaagcacggc gcgggctcgc gattcttcga
     3781 gcacgtgatg cgcgcctacg gctgcgtcaa caaggccgcg ccgtccttcg cgcagtgccg
     3841 tggcccgcgc gacatcggct acctgctcac ctacgggacc ggcctcggct ctcccgagcc
     3901 caccgacatc gccaacacga agtgcctggt gctcatcggc acgcacctcg gcgagaacat
     3961 gcacaacacg caggtgcagg aattcgcgca ggcgatcgcc aacaaggcga cgatcatcgt
     4021 ggtcgacccg cgcttttcgg tggccgcgag caaggcgaag cattggcttc cgataagacc
     4081 cggtacggat atcgccctgc tgctcgcgtg gatgaacgtg ctggtgaccg aaggcctgta
     4141 cgacaaggag ttcgtcgcga aatacggcca cggcttcgac aagttcaccg ccgccatcgc
     4201 cgccaacacg cccgaatggg ccgaaggcga gacgggcatc ccggcggcca ccatccgcgc
     4261 caccgcgcgc gagatggcga gccatcgccc cgccgccctc gtgcatcccg gacggcgcac
     4321 caactggaac ggcgacgata cgcagcgcgc ccgctcgacc gcgctcctca atgcgctcct
     4381 cggcaactgg ggccgcaagg gaggcctgta cctctcctcc ggcatgacgt tcgcgggcta
     4441 cccgttcccg aagtacccgg aagcgaagaa acccaaggtc gacaatcccg acgggcgctg
     4501 gccgttcgcc gacgaggaag tgaccaccgg gctgcgcgaa gcgacgatca cgggcaagcc
     4561 ctacccgatc aaggcgtggg tcgtgtacgc ctcgaacctc ctgcagacgc tgcccagcga
     4621 gaaggagacc ctcgaggcca tctcgaagct ggacctcctc gtcgtcgtgg acacgcaccc
     4681 gagcgagatc tccgggtacg cggacgtcgt gcttcccgac accatgttcc tcgagcgcca
     4741 cgacgacctc tacgtcggct gggggcggca gggatggacg gcgcttcgcc agcctgtcgt
     4801 cgcaccaccg ggcgagcaga agccggcttg gtggatcgcc aagcagcttg ccgaccggct
     4861 gggcgtgggc gagttcatgc ccttcaagga catggacgag tacctcgccg cgcgctgcgc
     4921 gaagaacggc atcgactacg cgaagctgcg caaggacggc atcctgatgg gcgagaagaa
     4981 gccgatcacc atcgaggaag gcgccgagat cgccttcgac acgccttcga agaaggtgga
     5041 gttctggtcc gaccagctcg ccaaggcagg cttcgacccg gtgccgaagt acaccgcgcc
     5101 cgaagccgcg cccgccgggt tctaccggct cgtcaccggc cgcgcgcccg tgcacacctt
     5161 cagccgcacg cagaccaacg tgtacctgcg cgacctgatg aaggaaaacg aggtgtgggt
     5221 gaacacggcc acggccgccc aggccggcct gaagcacggc cagtacgtga agctgaagaa
     5281 ccaggacggc gtggtgtcca accgggtgaa ggtgaaggcc acgcagcgca tccggcccga
     5341 cacgctgtac atggtctacg gattcgggca cacgaacaag atgctcaaga gcgcctacct
     5401 catgggcgcg agcgcggcgc agctcaacac gcgctacaag gtcgacccgc tgatgggcgg
     5461 cacgagcatc caccagaact tcgtcgcgtt cgaaaaggag gcgtgagatg ccacgcttcg
     5521 gaatggtcat cgacaccctg aagtgcgtgg gttgcatgga ttgcgtggtc gcctgccaga
     5581 cggaaaacga cgtgccccag ggccactgcc gcgactggat cgtcaccgag ttgcgcgggg
     5641 agttcccgac gctctcgctg gagatccgct cggagcgctg caaccactgc gacaacccgc
     5701 cttgcgtgtc gtgctgcccg acgggtgcga gccatgtcga ggatttcggc aagaccgtgc
     5761 aggtgacggc ggagaaatgc atcggctgca aggcctgcct gtcggcctgc ccgtacggcg
     5821 cgcgcttcgt ccaccccgac ggctatgcgg acaagtgcac cttctgcctg caccgcgtga
     5881 aggagggcaa ggatcccgcg tgcgtgtcgg tgtgcccgac ccgatgcatg cagttcgggg
     5941 acctggacga cccgaagagc gcggtgagcc ggctgctcgc ctcgcgcaag caccacacgc
     6001 tgctccccga agccggcacc aagccgcgcg tcttctacct ggcctgaggc gaccgccatg
     6061 ctcgaaatca ccaccacccg ccacaacccg ttcgtggacc ccatgctctc ggtctggagc
     6121 tgggagatcc ccgtctacct ttttctcggc ggcatgatcg ccgggatgat ggtcctcggg
     6181 ggcatcaaca tgctcaagct cgcccggggc gagcggccgg agggcttcta cagcgtgcag
     6241 actccggtgc tcgggttcgt gctgctcaac ctgggcatgg gcgcgctctt cctcgacctc
     6301 gcgcacaagc tctacgtcta ccgcgtgtac atggccttcg agccgacgtc gccgatgtca
     6361 tggggctcgt gggtgctgat cctcgtcttc ccggtcctca tcgtctccgc gctcatccgg
     6421 ctgcccgagg cgtggccgtg gctgggcgag cgcgtgcccg cggcgcgcgg ctggtccgcg
     6481 gcgctgctcg cgcgccccgg ggtcatcaag gcgctcggat acctgaacgt gttcctcggc
     6541 atcgggctgg gcatctacac cggcatcctg ctctccacga tggtcgcccg gccgctctgg
     6601 aacagcgcga tcctgggccc gctcttcctc gtctccggcc tttccgcggc cgcggcgatg
     6661 gtgcacctcg tggcgagctt cgtgccgggg catcccgcgc ctcgcggcct cgtcggcggc
     6721 gcactcgcgc tgctcgtcca gccgctcggc gagcgtcccc cggaacccgg cacggccacg
     6781 agcctcgttc gcgccgacct tgccttcctt gccgtcgaac tggtgctgat cgggctgctc
     6841 gtcatcggcc tgctgtcgag ttccgcctcc cacgcggccg cggcgcagat cattctctcg
     6901 gggaaattcg ccgtcctgtt ctgggccggc gtgatcgccg ccggaatcgt ggtcccgctc
     6961 ctgctgcaac tgctcgagct gggtcacagg atcccgcaca ccatcgtgcc cgccctcctc
     7021 gtgcttgccg ggggcctcgc ccttcgctgg gtcatggtgg gtgcgggcca ggcgagccac
     7081 gtgatcgccg cgaccggact gtaggaaagg aaaaaccatg accacccttg ccgcatccag
     7141 catctcctcc cgggccggcg caaagtcttc ggccgcgccg tactggaacc cctatgccgc
     7201 gggcttcggc ctggggctcg tcctccttgc ggcgtacctc gtcgccgggc gcggcctcgg
     7261 cgccaccggg gccttctcct cggtggccgc atggctcgcc aacgccatct cgccggaaca
     7321 cgcacaggcg agtgccgtcc acgcgcgcta tctcgagagc ggccccccgc tcacggcgtg
     7381 gctcacctgg ttgctgatcg gtaccttcat cggcgccgcc gtctccggcc tcgcggccgg
     7441 gcgcttcgcg atcagcgtgg agaaagggcc ccgcgtcacg gacggtcagc ggctggccct
     7501 cgccctcgcc ggcgggatca tcgccggcct gggtgccaag atcgcgcgcg gctgcacgag
     7561 cgggcaggcc ctcaccggtg cctccatcct caacccgggg agcctcgtct tcatgctcgc
     7621 cgtattcgtc gcgggctacg cggtggcctg gacgtaccga aaggagtggc tgtgatgttt
     7681 cccctttcct ccacgatgga attcacgcag gtcaccacgc tggcgcttgc cgccgtcatc
     7741 ggcatcggct tcggcttctt cctcgagaag gcgggattcg gcagcgcgcg aaagctgatg
     7801 gcggtgttct acctctacga catggccgtg gtgaaggtga tgttcaccgc gatcgtcacc
     7861 gcgatggtcg ggatcttcgt cctgtctacg gcaggcatgc tggatctcgc cgagctctat
     7921 atcgagccgt cgaactatcc ggcggcgatc atcggcggtt tggtcttcgg cgtgggattc
     7981 gtaatcggcg gctactgccc cggcacttcg ctcgccgcct gcgccaccgg ccgcctcgac
     8041 ggcttcgcct tcgtggccgg cattttcgcc ggctcctatg cctacgcgga attcctgtcc
     8101 gggttcgata cctggctcgc acaatccgcg caacccgact tgacgctgcc cctgctcacc
     8161 ggcatcccga tgggctggtt cgtgctcgcc ttcatcgcct tcctcgcgct cgccggctgg
     8221 ggcatgtcgg ccgcggagcg gcgcttcgcc gggttgcgac cctgattccg attcccccat
     8281 gacgcaaagg acagacacac catgaagatc aagcgccttt acagcgctct cgcgctggct
     8341 gggctcgtcg tgccatcggt cgccctggcg accgacggtt acttttccca cgcctacggg
     8401 ctcaagtccc tcggcatggg cggtgccgcg atcgccgttg ccagcgagcc gttcggcggc
     8461 gcggtgaatc cggcggcgat gacctcactg ggcaacgcct ggcaggcggg cgtgcaggtc
     8521 ttcatgccgc ggcgtgaagc cgagcgctcc ggctccggct tggcgggcat cgacggttcc
     8581 gtgtcgagcg acagcctcgc cttcgcgatt cccgagttcg gcttcaacta catggcgcgg
     8641 cccgacctgg ccgtcggcgt ctcggtggtc ggcaacggcg ggatgaacac cgacttccgc
     8701 ggcgaacaga ttccggccca gagcgcgtgc gccgcgttca atccggctcc cggcccgtac
     8761 aacctgcttt gcggcaacgg ccgcctcggc gtggatctca tgcaactcct ggtggcgccc
     8821 tacgccgcct ggaaggtctc gccctcgcac tcgatcggcg cagcccccgt ccttgcctac
     8881 cagcgcttcc gcgcgcaggg cctgcaggcc ttcgacaacc ccgggctgtc cacgagtcct
     8941 ggcgacgtca ccaatcgcgg ttacgcaagc gccacgggct acggcgtgcg cctcggctat
     9001 cacggcacct tcgacaagtt ctcggtcggg gtcgcctggg cgagcaagct ttcgatgggc
     9061 gagttcgaca agtacaaggg cctctttgcc gagcagggcg tcttcgacat cccgtccaac
     9121 ctcaccgtcg gcctcgcctg gcggccggac aacaagtggc tcgtcgcggt cgactgggag
     9181 cgcatcaagt actccgactc gaaggcggtt tccaacgcga gcgggctgct cctcaactgc
     9241 ttcggcggcg atcgcacctc gtgcatgggc ggctccaacg gtcccggctt cggctggaac
     9301 gacatcgatg tctggaagtt cgggttcgag tacgccgtcg atgcgaagct caccctgcgg
     9361 gccggctata accgcagcga gaaccccatc aacgcggccg acgtgacctt caacatcctg
     9421 gccccgggcg tcatcaagga ccactacacg gtcggcgcca cgtggaagct cgattcgcgc
     9481 tcggaaatca ccgcctacgg cctttacgcg tccaacaaca cggtgtccgg gacgagcctg
     9541 ttcgtgccct tcggcgctcc gccgacgacc agcgagacca ttcagctcaa ggaaatgacg
     9601 gtgggactcg gctacacctc ccacttctag tcttgcgtgc ctgacggggc cgcttgtccg
     9661 gggcctcgat tcgagcgaaa cggggcggcc aaggccgccc cgtttcgttt ccggcaacca
     9721 gcccgcccga ggcgggccgc gttcagccga gcaggtgctt caccgcggcg cgctcctcct
     9781 cgagttccgc gagcgtcttg tccatccgct ggcgcgagaa ctcgtcgatc tcgagtccct
     9841 tcacgatctg gtacttgccg ttggccgtcg tgcacgggaa gccgtacatc acgccctcgg
     9901 ggatgccgta ggatccgtcg gacaccacac ccatcgtcac ccatttgccg cccgagccca
     9961 gaacccagtc gtgcacgtgg tcgatcgccg cgttggctgc ggacgcggcg gacgaaagcc
    10021 cgcgcgcttc gatgatggcc gcgccgcgct tgccgaccag gggcatgaac gtgtccttgt
    10081 accaggcctc gtcgttcacc agcgccttca ccgactggcc gcccgcggtc gcgaagcggt
    10141 agtccggata catggtgggc gagtggttgc cccacacgac cagcttctcg aacgattcca
    10201 cgggcttgcc ggtcttcgcc gcgagttgcg aagcggcacg gttgtggtcc aggcgcatca
    10261 tcgaggtgaa gcaggtgggc gagaggctct tcgcgctttt catcgcgatg taggcgttgg
    10321 tgttggcggg gtttccgacc accagcaccc tcacgttgcg cgaggcgcct tcgtccaggg
    10381 cccggccctg gaccgtgaag atggcgccgt tggcttccag caggtccttc ctctccatgc
    10441 ccggcccgcg cggacgcgcg cccacgagga tcgccacgtc cgcgtccttg aacgcaacct
    10501 tggcgtcgga ggccgcgctc atccccgcga gcagcgggaa cgcgcaatcg tccagttcca
    10561 tcatcacgcc cttgagggcc ttctgcgcct tctcgtccgg gatctcgagc agttgcagga
    10621 tcaccggctg gtccttgccg agcatctcgc cgctcgcgat gcggaacagc aggctgtagc
    10681 cgatctggcc ggcggcaccg gtgacggcta cgcgcatggg cttattcatg ggggatctcc
    10741 taggttcttc gcgatggaaa ttcggggggt cgcccgcagg gcagcgctac ctggcgccgc
    10801 tcccgatggc cccgcgaaag tatatcgcag cgcagcaatc cgggccggcg caccatttgc
    10861 gccacgtcaa cagtccgcac cgccgccctg ctccgccttc gtcggtgcct tgaccaggga
    10921 gatggcgagc acg
//
LOCUS       DASUEV010000324         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1353940, whole genome shotgun sequence.
ACCESSION   DASUEV010000324 DASUEV010000000
VERSION     DASUEV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353940"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1409)
                     /locus_tag="VFV55_10265"
     CDS             complement(90..1409)
                     /locus_tag="VFV55_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX4944727.1"
                     /translation="MLEAPDLFSASGEANAPLAERLRPKRVEDVVGQRHLLGEGKPLA
                     VALASGKPHSMILWGPPGTGKTTLARLFARAFGAEFIAISAVLAGVKEIREAVDRARL
                     EREAHGRATILFVDEVHRFNKSQQDAFLPHVESGLFTFIGATTENPSFEVNGALLSRA
                     QVYVLESLAEEDLDLLIVRGFEAEAIDVTAEARKRIAEFADGDGRRALNLVEQVANSA
                     RAAARHGADAEFVDGVVTRAGRRFDKGGENFYDQISALHKSVRGSDPDAALYWMVRML
                     DGGADPRYLARRMIRMAAEDVGLADPRSLEIAMRAAEVYERLGSPEGELALAECVVYL
                     AVAPKSNAVYMAYNAAREFVSRDSSRPVPMHLRNAPTRLMKDLGYGKGYRYAHDEEGG
                     YAAGEHYLPDGMDAPGWYQPTDRGLEAKIREKLAWLRQRDKGTGEGE"
     gene            complement(1418..2065)
                     /gene="lolA"
                     /locus_tag="VFV55_10270"
     CDS             complement(1418..2065)
                     /gene="lolA"
                     /locus_tag="VFV55_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX4944728.1"
                     /translation="MRLARLALAALAAFVPLAAAAGAVDAFLAFTASSRTASARFEQQ
                     VFDRSGKQVDRSSGAFAFSRPGRFRWAYDKPVRTLIVADGTKLWIHDEDLNQVTVRPM
                     DRALSATPAALLAGQSDVTAVFTLREAGQADGLDWLEAVPKERDTGFERVRIGMKGPV
                     PAAMELHDSLGGRTVLRFPEFRPGAKVDAEAFRFTPPKGADVLDEMRPATSRPVR"
     gene            complement(2062..>2814)
                     /locus_tag="VFV55_10275"
     CDS             complement(2062..>2814)
                     /locus_tag="VFV55_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX4944729.1"
                     /translation="YNHKIREGIEKKQPLKHPFSLTPDNPEDLEALPFIVVVIDELAD
                     LMMVAGKKVEELIARLAQKSRASGIHLILATQRPSVDVITGLIKANVPTRVAFQVSSK
                     VDSRTILDQQGAESLLGQGDMLYLPPGAGHPQRVHGAYVADAEVQRVVEHLKRQGEAQ
                     YIDGILDDPELADADAGGDAPGGEADPLYDQAVQIVLQNRRASISLVQRHLRIGYNRA
                     ARLIEDMERAGLVSPMASNGNREILSPARMEK"
BASE COUNT          413 a          992 c          952 g          457 t
ORIGIN      
        1 aatgaattgc ctggtttatc cgtgttcatc cgtgttcatc cgtgtcctag cttttgggta
       61 gcgtcactcg ccatcgtcaa tcgccatcgt cactcgccct cgcccgtccc cttgtcgcgc
      121 tggcgcagcc aggcgagctt ctcgcggatc ttcgcctcga gcccccgatc ggtgggctgg
      181 taccagcccg gcgcatccat gccgtcgggc aggtagtgct cgccggccgc gtagccgcct
      241 tcctcgtcgt gggcgtaacg gtagcccttg ccgtagccca ggtccttcat gagccgggta
      301 ggcgcgttgc gcaggtgcat ggggacgggg cggctcgaat cgcgcgagac gaactcgcgg
      361 gcggcgttgt aggccatgta gacggcattg gacttcggcg ccaccgcgag atagaccacg
      421 cactcggcaa gggccagctc gccttccggg gaaccgaggc gctcgtagac ctcggccgcg
      481 cgcatcgcga tctcgaggga gcgcgggtcg gcgagcccga catcctccgc ggccatgcgg
      541 atcattcgcc gcgcgaggta acgcggatcg gcgcccccgt cgagcatgcg caccatccag
      601 tacaacgccg cgtccggatc gctgccgcgg accgacttgt gcaatgccga gatctggtcg
      661 tagaaattct ccccgccctt gtcgaaacgc cgccccgccc gcgtgaccac gccgtcgacg
      721 aattccgcgt ccgccccgtg cctcgcggcg gccctggcgg agttcgcgac ctgctcgacc
      781 aggttgagcg cgcgcctgcc gtcgccgtcg gcgaattccg cgatccgctt cctcgcctcc
      841 gcggtcacgt cgatcgcttc tgcctcgaac ccgcgcacga tcaggaggtc gaggtcctcc
      901 tccgcaaggc tttccagcac gtagacctgc gcccgcgaca ggagcgcacc gttcacctcg
      961 aaggacgggt tctcggtggt cgccccgatg aacgtgaaga gcccgctttc cacgtgcggc
     1021 aggaacgcgt cctgctgcga cttgttgaag cggtgcactt cgtccacgaa cagtatcgtg
     1081 gcgcgaccgt gcgcctcgcg ctccagccgc gcacggtcca ccgcctcgcg gatctccttc
     1141 acgccggcga gcaccgcgga aatggcgatg aactccgccc cgaacgcccg cgcgaacagg
     1201 cgggcgagcg tggtctttcc cgtcccgggc ggcccccaga ggatcatcga gtggggcttg
     1261 ccggaggcga gtgccacggc gaggggcttg ccctccccca gcaagtggcg ttgccccacg
     1321 acgtcctcga cgcgcttggg gcgcagccgc tcggcgagcg gcgcattggc ctcgccgctg
     1381 gcggaaaaga ggtccggggc ttcgagcatc ggcacgacta tcgaacggga cgggaagtcg
     1441 cgggccgcat ctcgtccagc acgtccgcac ccttgggcgg cgtgaaccgg aaggcttccg
     1501 catcgacctt ggcgcccggc cggaactcgg ggaagcgaag cacggtgcgc ccgccgagcg
     1561 aatcgtgcag ttccatggcg gccgggaccg ggcccttcat gccgatgcga acccgttcga
     1621 atccggtgtc gcgctccttg ggcaccgcct ccagccagtc aaggccatcg gcctgcccgg
     1681 cttcgcgcaa cgtgaagacg gccgtcacat cgctctgccc cgccagcagc gccgccggcg
     1741 tcgcggagag cgcacggtcc atgggcctca ccgtgacctg gttcaggtcc tcgtcgtgga
     1801 tccacagctt cgtcccgtcg gccacgatga gggtgcgcac cggcttgtcg taggcccagc
     1861 ggaaccgccc cggccgcgag aaggcgaacg cgcccgagga acggtcgacc tgcttccccg
     1921 agcggtcgaa cacctgttgc tcgaagcgcg cggacgcggt gcgcgacgag gcggtgaagg
     1981 cgaggaacgc atcgaccgcg cccgcggccg ccgccagcgg cacgaacgcg gccagcgccg
     2041 cgagggcgag tcgcgcgagc ctcacttctc catcctcgcg ggcgaaagga tctcccggtt
     2101 gccgttggag gccatcggcg agacgaggcc ggcgcgctcc atgtcctcga tcaggcgcgc
     2161 ggcccggttg tagccgatgc gcaggtggcg ctgcacgagg gagatcgagg cgcgccggtt
     2221 ctgcaggacg atctgcaccg cctggtcgta gagagggtcg gcctcgccgc ccggtgcgtc
     2281 tccgccggca tcggcatccg ccagttccgg atcgtcgagg atgccgtcga tgtactgcgc
     2341 ctcgccctgc cgcttgaggt gctcgacgac gcgctgcacc tccgcgtccg cgacataggc
     2401 gccatgcacg cgctgcggat gcccggcgcc cggcggcagg tagagcatgt cgccctggcc
     2461 gagcaggctc tcggcgccct gctggtcgag gatggtgcgc gaatcgacct tggacgaaac
     2521 ctggaacgcc acgcgcgtgg gcacgttggc cttgatgagg ccggtgatca cgtccaccga
     2581 cgggcgctgc gtggcgagga tgaggtggat gccggaggcg cgcgacttct gcgccagccg
     2641 cgcgatcagt tcctcgacct tcttgcccgc gaccatcatc aggtccgcga gttcgtcgat
     2701 caccacgacg atgaacggca gcgcctcgag gtcctcggga ttgtccggag taagcgagaa
     2761 cggatgcttg aggggctgct tcttctcgat gccttcgcgg atcttgtggt tgta
//
LOCUS       DASUEV010000325         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1355122, whole genome shotgun sequence.
ACCESSION   DASUEV010000325 DASUEV010000000
VERSION     DASUEV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355122"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFV55_10280"
     CDS             <1..1017
                     /locus_tag="VFV55_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX4944730.1"
                     /translation="DSRSAPDAGTTTVMAAALTFCLGAAVWYGYSGIVIAVAIVVTGL
                     LHYKTQLEGFSVRLNATDMASLLQFAVLSLVILPVVPNTGFGPYGALNPYNLWLMVVL
                     ISAVSLAGYVAWRLLGGKGPVLLLGALGGLVSSTATTLVYSRGARGKPDLEGTAATVI
                     LLANLVVLLRLSVLAVAVAPSILPTMLPVVAAGLLAGLPIAWSMWRKNAGGEAMENPE
                     LTNPTDLPVALGFGVAYAVVLVAAAWLNDLAGARGVYAVALVSGLTDVDAISLSSFRL
                     FGNASLTASGAVMAIVVALCSNIVVKATIVFVAGGASLGKRCITGFAGVAAALVAVAA
                     LMPS"
     gene            complement(1027..2730)
                     /locus_tag="VFV55_10285"
     CDS             complement(1027..2730)
                     /locus_tag="VFV55_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4944731.1"
                     /translation="MKQVVILGAGTGGALIANLLGQELDPREWVITVVDRGDIHVYQP
                     GLLFLPFGLYGHESANDIVRPIGEPLPRHVNFLAADVLRIDADKKTVETSITTLHYDF
                     LVCSLGCRVAPEEVEGLAEPLGRDGVHTFYTLEGAVAMRGALESMKEGRLLIDVCDMP
                     IKCPVAPIEFAFLADYYFTQKGIRDAIDITLVTPYAGAFTKPNANRVLTEIAKQKGVN
                     VVADFAASSVDAQAKTLKSFDGRTLDFDLLCAIPPNLGPQVIDDSGLGDGTGYALTDP
                     RTLKSRKADCVYFLGDNTNVSTSKAGSVAHFEAETIVENLLREIGGKPPLPTFDGHAN
                     CFIETGYHKAMLLDFNYDIEPLEGSFPMAHVGPFALLKESYLNHMGKLAFQWVYWNLL
                     LKGRLPNVPLLPSHMNFIGKDLETTPHARHAREMKVRDVMTKDVVSVHAGSSLTAAAT
                     LMVQKKVSGMPVLDVRDRLLGVLTEADFLSAMNLEQSAVTDALETVVRKRRARKDMGT
                     IVDDIMTTPPITIREDETLRAAVILMDTNRIKRLVVTDDEWKVRGVVSRADLIRLYAM
                     K"
BASE COUNT          400 a          910 c          978 g          527 t
ORIGIN      
        1 gactcccggt ctgcgcccga tgccggcacc acgacggtca tggcggcagc gctcaccttc
       61 tgcctgggcg ccgcggtctg gtacggctac tcgggaatcg tgattgcagt ggccatcgtg
      121 gtcacggggc tgctgcacta caagacgcag ctcgaaggct tctccgtgag gctcaatgcc
      181 acggacatgg cgagcctgct gcagttcgct gtgctctcgc tcgtgatcct gccggttgtg
      241 ccgaacacgg gcttcggccc ctacggcgca ctgaatccct acaacctctg gctcatggtg
      301 gtgctcattt ccgcagtgag cctcgcgggc tacgtggcgt ggcggctgct gggcggcaag
      361 gggccggtac tgctgctcgg agcgctcggc ggactcgtct cgagcaccgc caccaccctt
      421 gtgtactcgc gcggggcgcg aggcaagccg gatctcgagg gaaccgccgc caccgtgatc
      481 cttctcgcca atctcgtcgt gttgctcagg ctgtccgtcc tcgcggtggc cgttgcgccc
      541 agcatcctgc ccacgatgtt gccggtcgtg gccgcgggtc tcctggcggg cctgcccatc
      601 gcatggagca tgtggcgcaa gaacgccggc ggcgaggcaa tggagaaccc ggaactcacc
      661 aacccgacgg acctgcccgt cgcgctcggt ttcggcgtgg cctatgcggt agttctcgtg
      721 gccgccgcgt ggctgaacga cctcgcgggc gcccgcggag tgtatgccgt ggctctggtc
      781 tccggcctga ccgatgtcga cgcgatttcg ctctcgtcct tccgcctgtt cggcaacgcc
      841 agcctgactg cgtcgggcgc ggtgatggcc atcgtggtgg cgctctgctc caacatcgtg
      901 gtgaaggcga cgatcgtttt cgtggccggc ggcgcctccc tcggaaagcg atgcatcacc
      961 ggcttcgctg gcgtggctgc ggcgctggtg gccgtcgccg ccctcatgcc gtcctagccg
     1021 gccccgctac ttcatcgcgt agaggcggat caggtcggcg cgcgaaacca cgccgcggac
     1081 cttccattcg tcgtcggtca cgacgaggcg cttgatgcgg ttggtgtcca tgaggatcac
     1141 cgcggcgcgc agcgtctcgt cctcgcggat ggtgatgggc ggcgtggtca tgatgtcgtc
     1201 gacgatggtg cccatgtcct tgcgcgcgcg gcgcttgcgc accacggtct cgagcgcgtc
     1261 ggtgacggcg ctctgctcga ggttcatggc ggagaggaaa tcggcctcgg tgagcacgcc
     1321 cagcagccgg tcgcggacgt cgagcaccgg catgccgctc accttcttct gcaccatgag
     1381 cgtggccgcg gcggtaaggg agctgccggc gtgcacgctc accacgtcct tcgtcatcac
     1441 gtcgcgcacc ttcatctcgc gcgcgtgacg cgcgtgcggc gtggtctcga ggtccttgcc
     1501 gatgaagttc atgtgcgaag gcagcagcgg cacgttcggc aggcgcccct tgagcagcag
     1561 gttccagtac acccactgga aggcgagctt gcccatgtgg ttcaggtagc tttccttcag
     1621 cagcgcgaac ggccccacgt gcgccatcgg gaacgacccc tcgaggggct cgatgtcgta
     1681 gttgaagtcg agcagcatcg ccttgtggta accggtttcg atgaagcagt tggcgtggcc
     1741 gtcgaaggtc ggcagcggcg gcttgccccc gatctcgcgc agcaggttct cgacgatcgt
     1801 ctccgcctcg aagtgggcga cggagcccgc tttcgaggtg gacacgttgg tgttgtcgcc
     1861 gaggaagtag acgcagtcgg ccttccggct cttgagggtg cgcgggtcgg tgagcgcgta
     1921 gccggtgccg tcgcccaggc cggaatcgtc gatcacctgc gggccgaggt tgggcgggat
     1981 ggcgcacagc aggtcgaagt cgagggtgcg gccgtcgaag gacttgagcg tcttcgcttg
     2041 cgcgtccacc gagctggcgg cgaagtccgc gaccacgttc acccccttct gtttcgcgat
     2101 ctccgtgagg acgcggttgg cgttgggctt ggtgaaggcg cctgcatagg gcgtgacgag
     2161 cgtgatgtcg atcgcgtcac gaattccctt ctgcgtgaaa tagtagtcgg cgaggaaggc
     2221 gaactcgatg ggcgccaccg ggcacttgat cggcatgtcg cacacgtcga tcagcaggcg
     2281 gccttccttc atcgactcga gcgcgccgcg catcgccacc gcgccctcga gcgtgtagaa
     2341 ggtatgcacg ccgtccctgc ccaggggctc ggcgaggccc tccacctcct cgggcgccac
     2401 gcggcacccc agggagcaga cgaggaagtc gtaatgcagc gtggtgatgc tcgtctcgac
     2461 cgtcttcttg tccgcgtcga tgcgcaggac gtccgcggcc aggaagttca cgtgccgcgg
     2521 cagcggctcg ccgatgggcc gcacgatgtc gttcgcggat tcgtggccgt agagcccgaa
     2581 gggcaggaag agcaggcccg gctggtagac gtggatgtcg ccgcgatcga cgaccgtgat
     2641 cacccactcg cgcgggtcga gctcctggcc gaggaggttg gcgatgaggg cgccgccggt
     2701 gccggcgccg aggatgacga cctgcttcat ggatggcctc ccgagcgtga cgcctcgatt
     2761 ttcccggctg cgggcatgcc cggcttgtgg gaggtcaata atcgaacgct aatgt
//
LOCUS       DASUEV010000326        14294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1241521, whole genome shotgun sequence.
ACCESSION   DASUEV010000326 DASUEV010000000
VERSION     DASUEV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241521"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1068)
                     /gene="nagZ"
                     /locus_tag="VFV55_10290"
     CDS             complement(46..1068)
                     /gene="nagZ"
                     /locus_tag="VFV55_10290"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX4944732.1"
                     /translation="MRLGPVMIDVSGLELTAGDIERLQHPQVGGVILFARNFAAPLQL
                     IQLARSIREIRRPSLLIAVDHEGGRVQRFRRGFTAIPAMREFGRLFDRDPAQALAAAR
                     GCGFVIASELQAHGVDFTFAPVLDVDYGGSSVIGDRALHRDPDIIATLAEALQAGLSA
                     AGMASVGKHFPGHGYVRADSHLEVPVDDRTLAEISATDLVPFQRLARAGMGGVMPAHV
                     IYPKVDAKPAGFSLVWLRKVLRDGLHFDGLVFSDDLSMEGASTAGGMTGRANAALDAG
                     CDMVLVCNDPRAQDAVLEGLERRPVAPTLARRIGTMRGRAISNTALEASAAYLAAAEN
                     LARVRA"
     gene            complement(1079..1456)
                     /gene="acpS"
                     /locus_tag="VFV55_10295"
     CDS             complement(1079..1456)
                     /gene="acpS"
                     /locus_tag="VFV55_10295"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX4944733.1"
                     /translation="MIVGVGTDVVSIPRVAAALERWGERFANRILTPQERVRYAHTRQ
                     KASHLAKRFAAKEAFSKAIGTGIRAPFRWHCVSVGRDGRGKPGLVPSPDMARHLAEIG
                     VTAAHLSLSDDAGVAMAFVVLEG"
     gene            complement(1453..2226)
                     /locus_tag="VFV55_10300"
     CDS             complement(1453..2226)
                     /locus_tag="VFV55_10300"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX4944734.1"
                     /translation="MIELGVNIDHIATVRQARRTYEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDEDVRRLRELTHIKLNLEMAATEEMVGVALRVKPEMAMLVPEGRQEVTTE
                     GGLDIAGQEARIRDVVARLAGAGIVTSVFIDADMRQVEAAGRVGARVCEIHSGPYAHA
                     FHSRGRDPQSAPVVAELDKIRAAGEAIRALGMRFNAGHALNYFNVEPVAALPGVRELH
                     IGHAIVSRAVFVGMREAVREMKALMVAAAGHALLKARAR"
     gene            complement(2223..3020)
                     /gene="recO"
                     /locus_tag="VFV55_10305"
     CDS             complement(2223..3020)
                     /gene="recO"
                     /locus_tag="VFV55_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX4944735.1"
                     /translation="MSAPGTSPDRGYVLHTYPYRETSLILQVWTEKHGRFAVVAKGAR
                     RPRSATRGVLVPFQPLAFTWFGRGEVKTLKTAEPAGAALPLAGASLLSAFYLNELLLK
                     LIHRDDPHEGLWVAYDEAVTSLRERSRRAAGVPGLLAAEPGVGEPRAIEPVLRRFELA
                     MLRELGYAVELGRDAAGGAAIDPARDYWYVMERGPVPADTAGERADAVRLSGLTLVHL
                     DRGIFDDARTAGQAKSLMRMLIHHCLNGQELTTRAIVRDLQRIEDAA"
     gene            complement(3017..3907)
                     /gene="era"
                     /locus_tag="VFV55_10310"
     CDS             complement(3017..3907)
                     /gene="era"
                     /locus_tag="VFV55_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX4944736.1"
                     /translation="MTAADFRAGTVAIVGRPNVGKSTLLNQLVGAHVSITSRKAQTTR
                     HRILGVRTGEGAQLVFVDTPGFQTRYSNALNRAMNRAVTSTLASVDAIVMVVEAAGWD
                     ERDDPVLALIPAGAPVVLAVNKVDRVKDRARLARLLVACAAKRDFAALVPVSAEKGTQ
                     VNALADEVAKLLPLAGPLYGPDDVTDRPERFHAAELVRERLFRLLGDELPYTTAVVID
                     EFREEGSLRRISATIYVDRQSHKAIVIGDKGATLKRIGTEARVAMESLFGGKVFLELW
                     VKVKGGWADSEALVRQWGYE"
     gene            complement(3904..4611)
                     /gene="rnc"
                     /locus_tag="VFV55_10315"
     CDS             complement(3904..4611)
                     /gene="rnc"
                     /locus_tag="VFV55_10315"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX4944737.1"
                     /translation="MPRRAAVRDLSGLQRRLGYVFRDPALLRRALTHRSHAADHNERL
                     EFVGDSLLNCAVAILLFERLPGVPEGDLSRLRAGLVNQSSLCEIAQTLELGDDLLLGE
                     GELKSGGFRRPSILADAFEALLGAVYLDAGFDAVRGVVESLVGGQIDRAREAPPAKDP
                     KTALQENLQGRRLPLPRYAVLAVGGEAHRQVFRVECRVDELGVTGVGEGASRRAAEQQ
                     AAEAVLGQLGEKKAGPP"
     gene            complement(4617..4973)
                     /locus_tag="VFV55_10320"
     CDS             complement(4617..4973)
                     /locus_tag="VFV55_10320"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX4944738.1"
                     /translation="MTRTQRGFTLSGALMGMVVLALAALFAAKLLPSYIEYYAVQKIL
                     KAMETTGDTKGTVQDIRRSFDRRNAIEDVKSVVGDDLDITKEGGEAVVTATWSTKVPI
                     IGNFSACLDFSVTTAK"
     gene            complement(5019..5837)
                     /gene="lepB"
                     /locus_tag="VFV55_10325"
     CDS             complement(5019..5837)
                     /gene="lepB"
                     /locus_tag="VFV55_10325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX4944739.1"
                     /translation="MGFLLKFTDFAAVLLVAAVATGLIWLVDRKVLAPKRAEGAAEPI
                     VVDMARAFFPVIVVVFLIRSFWVEPFKIPSGSMKPTLLVGDFILVNKYTYGIRLPVLN
                     RKVIEVGEVKRGDVVVFRYPLDTSVDYIKRVVGLPGDKVTYQGKLLTINGIKIPVQPA
                     GYYTDAELNFVRLPTFTEKLGEKTHAMMVVPPEPVLNLSQVRQFPHRENCEYNDDGFT
                     CTVPPGHYFMMGDNRDQSADSRYWGFVPDDHIKGRAFLVWMNFGDLKRVGNGIE"
     gene            complement(5994..7787)
                     /gene="lepA"
                     /locus_tag="VFV55_10330"
     CDS             complement(5994..7787)
                     /gene="lepA"
                     /locus_tag="VFV55_10330"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX4944740.1"
                     /translation="MQRIRNFSIIAHIDHGKSTLADRIIERTGGLSTREMDAQVLDSM
                     DLERERGITIKAQTAALSYTARDGREYRLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTATELGVEVVPVLNKIDLPSAQPERVIQEIEDVIGIDATDAIR
                     CSAKTGEGVDDVIEGVIARIPPPAGNPDGPLKALIIDSWFDNYVGVVMLVRVVDGALK
                     PKDRILLMATGAQHLCEQVGVFTPKSLARESLSAGEVGFVIAGIKDLKDAKVGDTVTL
                     VERRADAALPGFKEIKPQVFAGLYPIESNQYEALRDALTKLQLNDSSLRFEPETSQAL
                     GFGFRCGFLGLLHMDIVQERLEREYDMELITTAPTVVYQVALRDGTVIEIENPSKLPD
                     ASRIEEIREPIIRATVLMPQEYVGPVITLCTQKRGTQVNMQYVGRQVILTYDMPLNEV
                     VMDFFDKLKSVSRGYASLDYEFREFRAGDLVKLDIQINGEKVDALSLIVHREKSVYRG
                     RDLASRMRKLIPRQMFDVAVQAAIGANIIARENISALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAILQVEGK"
     gene            complement(7907..8152)
                     /locus_tag="VFV55_10335"
     CDS             complement(7907..8152)
                     /locus_tag="VFV55_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX4944741.1"
                     /translation="MSPTLTVLSRGYCHLCHQMIAELEKLRGRHDFRIEVVDVDRDPA
                     LEEKWGDKVPVLLDGGREICHYFLDVEALEARLVRMK"
     gene            complement(8149..9609)
                     /locus_tag="VFV55_10340"
     CDS             complement(8149..9609)
                     /locus_tag="VFV55_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX4944742.1"
                     /translation="MQTTKAFLLGAALVLGLATQAQAQSQQVLPNFADLVEKHGPAVV
                     NIRTEARAQRTQIPGLSEDDPMAEFFRRFMPPDQRGAPRGRDDKGDKGDRGGPRAPLR
                     PFGLGSGFVISADGYIVTNAHVIDNADEIYVRFIDKREMKAKVIGADKRSDVALIKVD
                     ATNLPLIKLGDSHNTRVGEWVLAIGSPFGFANSVTAGILSAKSRDLPADSTASDAVPF
                     LQTDVAVNPGNSGGPLFNLKGEVIGINSQIFSRTGSYAGISFAIPIDYAANVIDQLKK
                     TGKVTRGRIGVAIQNVSRDLADSLGLARTDGAAVGTVEEDSPAAKAGIEVGDVILKID
                     GRAVESSADLSRTVRAVRPGQKITLTVWRTGKTRDHVVTVGEFKDEEATRTAARSKKE
                     TAKPGKLGLAVAEASAEQRKALKIQGGVAVEAVDGASLAAGIQPGDVILRVNNVDIKD
                     VKGYNDALAKLDPKKPVALLIRDENGTRFVTLKAED"
     gene            complement(9643..10635)
                     /locus_tag="VFV55_10345"
     CDS             complement(9643..10635)
                     /locus_tag="VFV55_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX4944743.1"
                     /translation="MRRQAAALASLALGAGLAAVADPGSDPVTWLQRAATSARQTTYA
                     GTVMHMQGERTATSRITHVFVSGSEHEKIESLDGPRREIVRHDDQMQCFFPDAKTIRI
                     DRRVTAKFFPSLVGGPMEAIAENYKLALGNVERVLGQDCQWILLDPKDALRYAQRLCA
                     EVGSGLLLRAKTLGAKQQVLEQYAFTDLRVGRDVSRGEVKSTFLPQSKDWRRDVQPPD
                     EPNHASTGWSVALPPPGFRLVGEMQRKLPNRAHPVTQLVLSDGIATMSVFIEPMSNPP
                     RTAEAANEDGALSVFVRPMGENIVTVLGEVPPSAAQQVGRSIARHAAVASGAAR"
     gene            complement(10632..11120)
                     /locus_tag="VFV55_10350"
     CDS             complement(10632..11120)
                     /locus_tag="VFV55_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX4944744.1"
                     /translation="MTQELSSLMDGELGAQEAERAIRNCCGDEELRHKWRTYHLIGEA
                     LRGEGTCASTSTRRIMEAIGGEPTVLAPRRRVSASAGRIAFAAAASVATVAVVGWIGL
                     QDRGTPTGPAVALSSPATPPPVVADNVVPLKNVNEYVVVHRQLPNADFYRPVANQAAA
                     VR"
     gene            complement(11200..11799)
                     /gene="rpoE"
                     /locus_tag="VFV55_10355"
     CDS             complement(11200..11799)
                     /gene="rpoE"
                     /locus_tag="VFV55_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEX4944745.1"
                     /translation="MSDREIDQQLVERAQRGEKRAFELLVIKYQRKLERLLSRIIRDP
                     NEIEDVAQEAFVKAYRALPNFRGDSAFYTWLYRIGINTAKNYLIAQGRRAPTSTEYDS
                     EEAEGFEDAAGLRDINTPENLLMSKQIGKTVNDAIDALPGELRTAITLREIDGLSYEE
                     IAKIMDCPIGTVRSRIFRAREAVAERLRPQLGTSKDHRW"
     gene            12021..13607
                     /gene="nadB"
                     /locus_tag="VFV55_10360"
     CDS             12021..13607
                     /gene="nadB"
                     /locus_tag="VFV55_10360"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX4944746.1"
                     /translation="MITYDVVVIGSGLAGLSAALRLAPTRKVALVTKRELLDGASSHA
                     QGGIAAVLDSADSLENHIRDTHVAGGGLCHDDAVRFVVERGRQSIQWLVDQGVPFTRE
                     AGGTEELHLHREGGHSHRRIVHAADATGNAVQRTLLQQVKAHPNIEILEWHLAIDLIT
                     GRKFGVGDDRCYGAYVLDLKAGKVKTLAARSTILATGGIGKVYLYTTNPDTATGDGIA
                     MGWRAGCPVANMEFIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPNGERFMPRHDER
                     GELAPRDVVARAIDFEMKKRGLDCVYLDISHKGEAFVREHFPNIHAKCLEFGIDIGKE
                     PIPVVPAAHYSCGGLQVDLAGRTPLAGLYALGEVACTGLHGANRLASNSLLECLVFSE
                     AAVADILAGTPVTVPPLPAWDESRVTDADEEVVIAHNWDELRRSMWSYVGIVRTNKRL
                     ERALHRIQLLQAEIHEFYSAFRVSNDLIELRNLVVTAELVVRSALARKESRGLHFSSD
                     WPDMLPVARDTVLHPRPVEK"
     gene            13604..>14294
                     /locus_tag="VFV55_10365"
     CDS             13604..>14294
                     /locus_tag="VFV55_10365"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX4944747.1"
                     /translation="MSALPLLTTAQVRSLEERAARGLAGPTLMQRAGRATADAALALA
                     ADTGEPILVVAGPGNNGGDAWVAAAQLRESFHRVTVLDVAGTEPKAPEAREARARFLA
                     GGGQAVRQWPAIPRPALVIDGLLGIGLARDVDGELAALVAHINDCGAPVLAIDIPSGI
                     ASDTGRVRGVAVRAARTITFIARKVGLYTGDALDHCGEIVLDDLGLDAALLADTPGSL
                     LTPESVRGWLAP"
BASE COUNT         2143 a         4988 c         4791 g         2372 t
ORIGIN      
        1 gcggtaaagt aacctgaccc ctttctgcaa agtaacctgc cccctttatg cgcgaacgcg
       61 cgccaggttc tcggcggcgg caaggtaggc ggcgctcgcc tcgagggcgg tgttggagat
      121 ggcgcggccg cgcatggtcc cgatgcggcg cgcgagggtg ggtgcaaccg ggcggcgctc
      181 cagtccttcc agcaccgcgt cctgcgcgcg cgggtcattg cagactagga ccatatcgca
      241 gccggcatcg agggccgcat tggcccgccc ggtcatcccg cccgcggtgc tcgcgccttc
      301 catcgagagg tcgtcggaaa agaccagtcc gtcgaagtgc aaaccgtcgc gcagcacctt
      361 ccgcagccac accagcgaga atccggccgg cttcgcgtcc accttcgggt agattacgtg
      421 cgccggcatg acgccgccca ttcccgcgcg tgccaggcgc tggaagggca cgagatccgt
      481 cgccgatatc tcggccagcg tgcgatcgtc caccggcacc tcgaggtggg aatcggcgcg
      541 cacgtagccg tgcccgggaa agtgcttgcc gacggaggcc atgcccgctg cggaaagccc
      601 cgcctgcagc gcctccgcaa gagttgcgat gatgtccggg tcgcggtgca gcgcgcggtc
      661 gccgatgacg ctcgacccgc cgtagtccac gtccagcacc ggggcgaagg tgaagtcgac
      721 gccatgggcc tgcagctcgc tcgcaatcac gaagccgcag ccacgcgcag cggcgagcgc
      781 ttgcgcgggg tcgcggtcga aaaggcgtcc gaactcgcgc atcgcgggta tcgcggtgaa
      841 cccgcggcgg aagcgctgca cgcgccctcc ctcgtggtcc acggcgatga ggagcgaggg
      901 cctgcggatc tcccggatcg agcgcgcgag ttggatcaac tgcagtggcg ccgcgaagtt
      961 cctcgcgaag aggatcacgc cgcccacctg cgggtgctgc aggcgttcga tgtcgccggc
     1021 cgtgagctcc agccccgata cgtcgatcat caccggtccg agccgcatgg gtgcctttct
     1081 agccttcgag aacgacgaac gccatcgcca cgccggcatc gtcggaaagc gaaagatgcg
     1141 cggccgtaac cccgatctcg gcgaggtggc gggccatgtc cgggctggga acgagtccgg
     1201 gcttgccgcg cccgtcgcgc ccgaccgaga cgcaatgcca gcggaacggc gcgcggatac
     1261 ccgtgccgat cgccttcgag aacgcttcct tggcggcgaa ccgcttggcc agatggctcg
     1321 ccttctgccg cgtgtgcgcg tagcgcaccc gctcctgcgg cgtgaggatg cggttcgcga
     1381 accgctcgcc ccagcgctcg agcgccgcgg ccacccgcgg gatcgagacc acgtcggtgc
     1441 ccacgcccac gatcatcgcg cccgtgcctt gaggagcgcg tgcccggcgg ccgcgaccat
     1501 cagcgccttc atctcgcgca ccgcctcgcg catgcccacg aagacggcac ggctcacgat
     1561 ggcgtggccg atgtgcagct cgcgcacgcc cggcagtgcg gccacgggct ccacgttgaa
     1621 gtaattgagg gcgtgcccgg cattgaagcg catgcccagg gcgcggatcg cctcgccggc
     1681 cgcgcggatc ttgtccagct ccgcgacgac cggcgcgctc tgcgggtcgc gtccccggga
     1741 atggaaggca tgggcatagg ggccggagtg gatctcgcac acgcgcgccc ccacgcgccc
     1801 cgccgcctcc acctggcgca tgtccgcatc gatgaagacg ctggtcacga tgcccgcgcc
     1861 ggcgaggcgg gcgaccacgt cgcggatgcg cgcctcctgc ccggcgatgt ccaggccgcc
     1921 ttccgtggtg acttcctgcc gcccctcggg aacgagcatc gccatttccg gcttcacgcg
     1981 cagggccacg ccgaccatct cctcggtggc ggccatctcc aggttgagct tgatgtgcgt
     2041 gagctcgcgc aggcgccgca cgtcctcgtc ctggatgtgg cggcggtcct cgcgcaggtg
     2101 caccgtgatg ccgtccgcgc cgcccaggtg cgcctcgaca gcggcccaga cggggtcggg
     2161 ttcgtaggtg cgccgtgcct gccgcaccgt ggcgatgtgg tcgatgttga cgccgagctc
     2221 gatcatgccg cgtcctcgat tctctgcaag tcgcgcacga tggcccgcgt ggtgagttcc
     2281 tgcccgttga ggcaatggtg gatgagcatt cgcatgaggc tcttggcctg ccccgccgtg
     2341 cgcgcgtcgt cgaagattcc gcggtcgagg tgcaccaggg tgaggccgga caatcttact
     2401 gcatcggccc gctcccccgc cgtgtcggcc ggcaccggac cgcgctccat cacgtaccag
     2461 tagtcgcgcg ccggatcgat ggctgccccg cccgcggcat cgcgtcccag ttccaccgca
     2521 tagcccagct cgcgcagcat cgccagctcg aagcggcgca gcaccggctc gatcgcgcgc
     2581 ggctcgccca cgccgggttc cgccgccagg agcccgggca cgccggccgc gcgccgcgag
     2641 cgctcgcgca gggacgtcac cgcctcgtcg taggcgaccc aaagcccctc gtgcgggtcg
     2701 tcgcgatgga tgagcttgag cagcagctcg ttgaggtaga acgccgagag caggctcgcg
     2761 cccgcgagcg gcagcgcggc tccggcaggc tcggcggtct tcagcgtctt cacctcgccg
     2821 cgcccgaacc aggtgaacgc aagcggctgg aagggcacga ggacgccgcg ggtcgcgctc
     2881 ctgggccggc gcgcgccctt ggccaccacg gcgaaacggc cgtgcttctc cgtccagacc
     2941 tgcaggatga ggctcgtctc gcggtacgga taggtatgca ggacgtagcc gcggtcggga
     3001 ctggtgccgg gcgcgctcac tcgtagcccc actggcggac cagcgcctcg ctgtcggccc
     3061 agccgccctt caccttcacc cacagctcga ggaacacctt gcccccgaag aggctctcca
     3121 tcgccacgcg cgcttccgtg ccgatgcgct tgagcgtggc gcccttgtcg ccgatgacga
     3181 tcgccttgtg gctctgccgg tccacgtaga tggtggcgga aatgcggcgc agcgaccctt
     3241 cctccctgaa ttcgtcgatc acgaccgccg tggtgtaggg aagctcgtcg cccagcaggc
     3301 ggaagagcct ctcgcgaacc agctccgcgg cgtggaaccg ctcgggacgg tcggtgacgt
     3361 cgtcgggacc gtagagcggc ccggccagcg gcagcagctt cgccacctcg tcggccagcg
     3421 cgttcacctg cgtgcccttc tccgcgctca ccggcacgag tgccgcgaaa tcgcgcttcg
     3481 cggcgcacgc gacgagaagg cgggcgagcc gggccctgtc cttcacgcgg tccaccttgt
     3541 tgaccgccag cacgaccggc gcgcccgccg ggatgagggc gagcaccggg tcgtcgcgct
     3601 cgtcccagcc ggcggcctcc accaccatca cgatcgcatc cacgcttgcg agcgtcgagg
     3661 tgacggcgcg gttcatggcg cggttgaggg cgttggaata gcgcgtctgg aaacccggcg
     3721 tatcgacgaa cacgagctgc gccccctcgc cggtgcgcac cccgaggatg cggtggcggg
     3781 tggtctgcgc cttgcgcgac gtgatggaga cgtgcgcgcc gacgagctgg ttgaggagcg
     3841 tcgacttgcc cacgttgggc cggccgacga tggctaccgt gccggcgcgg aaatccgcgg
     3901 cggtcacggc ggccccgcct tcttctctcc gagctgtccc agcaccgcct cggctgcctg
     3961 ctgttccgcg gcgcggcggc tcgcaccctc ccccacgccc gtcacgccga gttcgtccac
     4021 gcggcattcg acgcggaaga cctgccggtg cgcctcgcct cccacggcga gaaccgcgta
     4081 gcgcggcagc ggcaggcgcc tgccctgcag gttttcctgc aatgcggtct tcgggtcctt
     4141 ggccggcggg gcctcgcgtg cacggtcgat ctggccaccg acgagcgatt cgacgacgcc
     4201 gcgcaccgcg tcgaacccgg catcgaggta caccgcgccc aggagcgcct cgaaggcgtc
     4261 ggcgaggatc gacgggcggc ggaacccgcc gctcttgagc tcgccctcgc ccaggagcag
     4321 gtcgtcgccc aactcgaggg tctgcgcgat ctcgcacagg gaggactggt tcaccaggcc
     4381 cgcgcgcagc cgcgagaggt cgccttcggg caccccgggc aggcgttcga aaagcaggat
     4441 cgccaccgcg caattgagca ggctgtcgcc cacgaactcg aggcgctcgt tgtggtcggc
     4501 cgcgtggctg cggtgcgtga gggcccggcg caggagcgcc ggatcgcgga agacgtatcc
     4561 gaggcggcgc tgcaggccgg agagatcgcg cacggccgcg cgtcggggca cggcgcctac
     4621 ttcgcggtgg tcaccgagaa atccaggcag gcgctgaagt ttccgatgat cggcaccttg
     4681 gtcgaccagg tggcggtcac gacggcttcg ccgccttcct tcgtgatgtc caggtcatcg
     4741 ccaaccacgg atttcacgtc ctcgatggca ttgcgcctgt cgaacgacct gcggatatcc
     4801 tgcacggtgc ccttggtatc gcccgtggtt tccatggcct tgaggatctt ctgcaccgca
     4861 tagtattcga tgtacgaggg caggagcttc gccgcgaaga gggccgcgag cgctagaacg
     4921 accatgccca tcagggcccc ggatagcgtg aagccgcgct gcgttcttgt catgaatccc
     4981 gtctcccttg cgaccttccc gcccgtgcgg gaagcggcct attcgatccc gttgcccacg
     5041 cgcttgaggt ccccgaagtt catccagacg aggaacgcgc ggcccttgat gtgatcatcc
     5101 gggacgaagc cccagtagcg gctgtcggca ctctggtccc ggttgtcgcc catcatgaag
     5161 tagtgaccgg ggggcaccgt gcaggtgaac ccgtcgtcat tgtattcgca attttcgcgg
     5221 tgcggaaatt gccggacctg cgagaggttc agcaccggct cgggaggcac gaccatcatc
     5281 gcgtgcgtct tctcgccgag cttctcggtg aaggtcggca ggcgcacgaa attgagttcc
     5341 gcgtcggtgt agtaccccgc cggctgcacc gggatcttga tcccgttgat cgtgagaagc
     5401 ttgccctggt aggtgacctt gtccccgggc aggcccacga cgcgcttgat gtagtcgacc
     5461 gacgtgtcca ggggatagcg gaagaccacc acgtcgccgc gcttgacctc gcccacctcg
     5521 atcaccttcc ggttgaggac cggcaggcgg atgccgtagg tgtacttgtt cacgaggatg
     5581 aagtcgccca ccagcagcgt gggcttcatc gagcccgacg ggatcttgaa cggctccacc
     5641 cagaacgagc ggatgaggaa cacgaccacg atcaccggga agaaggcgcg cgccatgtcc
     5701 accacgatcg gctcggcggc gccttccgcg cgcttcggcg cgagcacctt gcggtcgacg
     5761 agccagatga ggcccgtggc caccgccgct acgaggagca ccgcggcgaa gtcggtgaac
     5821 ttgagcagga agcccatgag cgcgatgaac agcaccggcc aggcgatctc gagggttccc
     5881 ttgagcgagt ccgatcgcgg cccgctgcgg atcgccgcat cccagccgat gagcgccgcg
     5941 gaggcgccgc cggccacgag cgcgatgaat tcccagttca gtccaccctg catctacttg
     6001 ccctccacct gcaggatggc gaggaacgcc tcctgcggaa tctcgaccga gccgacctgc
     6061 ttcatgcgct tcttgcccgc cttctgcttc tcgaggagct tcttcttgcg cgtgatgtcg
     6121 ccgccgtagc acttggcgag cacgttcttc ctcagcgccg agatgttctc gcgcgcgatg
     6181 atgttggccc cgatcgccgc ctgcaccgcc acgtcgaaca tctgcctcgg gatgagcttt
     6241 cgcatgcgcg aagcgaggtc gcggccccgg tacacggact tctcgcggtg cacgatgagc
     6301 gacagggcgt cgaccttctc gccgttgatc tggatgtcga gcttgaccag gtccccggcg
     6361 cggaactcgc ggaactcgta gtcgagcgag gcgtagccgc gcgatacgct cttcagcttg
     6421 tcgaagaagt ccatgaccac ctcgttgagg ggcatgtcgt aggtgaggat cacctgccgg
     6481 ccgacgtact gcatgttcac ctgcgtcccg cgcttctgcg tgcacagcgt gatcaccggc
     6541 cccacgtact cctggggcat gaggacggtc gcgcggatga tgggctcgcg gatctcctcg
     6601 atgcgcgagg cgtcgggcag cttcgacggg ttctcgatct cgatgacggt cccgtcgcgc
     6661 agcgccacct ggtagaccac ggtcggcgcc gtggtgatga gctccatgtc gtactcgcgc
     6721 tcgagccgct cctggacgat gtccatgtgc agcaggccca ggaagccgca gcggaagccg
     6781 aagcccagcg cctggctggt ctcgggctcg aagcgcaggc tcgagtcgtt gagctggagc
     6841 ttggtcagcg cgtcgcgcag cgcctcgtac tggttcgatt cgatcgggta gaggccggcg
     6901 aagacctgcg gcttgatctc cttgaagccg ggaagcgccg cgtccgcgcg ccgctcgacg
     6961 agcgtcacgg tgtcgcccac cttcgcgtcc ttcaggtcct tgatgccggc gatcacgaag
     7021 cccacctcgc ccgccgaaag gctctcgcgc gccagggact tgggcgtgaa gacgcccacc
     7081 tgctcgcaca ggtgctgcgc cccggtggcc atgagcagga tgcggtcctt cggtttcagg
     7141 gccccgtcga ccacgcgcac cagcatcacc acgcccacgt aattgtcgaa ccacgagtcg
     7201 atgatgaggg ccttgagcgg cccgtcgggg ttgcccgcgg gaggaggaat cctcgcgatc
     7261 acgccctcga tcacgtcgtc cacgccctcg cccgtcttcg cgctgcagcg gatggcgtcg
     7321 gtcgcgtcga tgccgatgac gtcctcgatc tcctggatca cgcgctcggg ctgggccgag
     7381 ggcaggtcga tcttgttgag caccggcacc acctcgacgc ccagttcggt ggcggtgtag
     7441 cagttggcga ccgtctgggc ttccacgccc tgggaggcgt cgaccaccag gagcgcgccc
     7501 tcgcaggccg acagcgagcg gctcacttcg taggaaaagt ccacgtgacc cggagtgtcg
     7561 atgaggttca gccggtattc ccgcccgtcc ctcgcggtgt agctgagcgc ggccgtctgc
     7621 gccttgatcg tgatcccgcg ttcgcgctcc aggtccatcg aatccagcac ctgcgcgtcc
     7681 atttcgcgcg tggacaggcc cccggtgcgc tcgatgatgc ggtccgcgag cgtcgacttg
     7741 ccgtggtcga tgtgggcgat gatggaaaag ttgcggatgc gctgcatggt cgtggccgga
     7801 aaaggggacg caacccttta ccgatgattt ccggaaaagg gttgcgtccc cttttccatc
     7861 aagacaaagg gcacgccgga cgtgcccttt tttgcagtgc aatattctac ttcatccgga
     7921 ccaggcgcgc ttcgagggcc tcgacgtcca ggaagtagtg gcaaatctcg cgcccaccgt
     7981 cgagaagcac tggcaccttg tcgccccatt tttcctcgag ggcggggtcg cggtcgacat
     8041 ccaccacctc gatgcggaag tcgtggcgac cgcggagctt ttccagctcc gcgatcatct
     8101 ggtggcagag gtggcagtac ccgcgtgaca ggacggtgag cgtcgggctc aatcctcggc
     8161 cttgagcgtc acgaaccggg tgccgttctc gtcgcggatg agcagcgcca ccggtttctt
     8221 cggatcgagc ttggccagcg cgtcgttgta gcccttcacg tccttgatgt cgacgttgtt
     8281 cacgcgcagg atcacgtcac cgggctggat gcccgcggcg agcgaggcgc cgtccacggc
     8341 ctctaccgcc acgccgccct ggatcttgag cgccttcctc tgctcggcgc tcgcctcggc
     8401 gaccgcgagg ccgagcttgc cgggcttggc cgtctccttc ttgctgcgcg cggcggtgcg
     8461 cgtggcttcc tcgtccttga actcgcccac cgtgaccacg tggtcgcgcg tcttgccggt
     8521 gcgccagacg gtaagcgtga tcttctgccc cggacgcacg gcgcgcacgg tgcgcgacag
     8581 gtccgcggaa ctctcgacgg cacgtccgtc gatcttgagg atcacgtcgc ccacctcgat
     8641 tcccgccttg gcggccggcg aatcctcctc caccgttccg acggcggcgc catcggtcct
     8701 cgccagcccg agcgaatcgg ccaggtcgcg cgagacgttc tggatcgcca cgccgatgcg
     8761 cccgcgcgtg accttgccgg tcttcttgag ctggtcgatc acgttggccg cgtagtcgat
     8821 ggggatcgcg aaggagatgc ccgcatagct gcccgtgcgc gagaagatct gcgaattgat
     8881 gccgatcacc tcgcccttca ggttgaagag gggcccgccg gagttgccgg ggttcaccgc
     8941 gacgtccgtc tgcaggaacg gcacggcatc gctcgcggtg gaatccgcgg gcaggtcgcg
     9001 gctcttggcc gaaaggatcc ccgcggtcac cgagttggcg aagccgaagg gcgagccgat
     9061 ggcgagcacc cattcgccca cgcgcgtgtt gtgcgagtcg ccgagcttga tcaacgggag
     9121 gttggtcgcg tccaccttga tgagcgccac gtcggagcgc ttgtcggcgc cgatcacctt
     9181 ggccttcatc tcgcgcttgt cgatgaagcg cacgtagatc tcgtcggcgt tgtcgatcac
     9241 gtgcgcgttg gtgacgatgt agccgtcggc cgagatcacg aaaccggagc cgaggccgaa
     9301 cggacgcagc ggcgcgcgcg gcccgcccct gtcgcccttg tcgcccttgt catcgcggcc
     9361 gcgcggggcg ccgcgctggt ccggcggcat gaagcggcgg aagaactcgg ccatcgggtc
     9421 gtcttccgac aggcccggaa tctgcgtgcg ctgcgcgcgc gcctcggtgc ggatgttcac
     9481 gaccgccggg ccgtgctttt ccacgaggtc ggcgaagttg ggcagcacct gctgcgattg
     9541 cgcctgggcc tgggtggcca ggccgagtac cagcgcggcg cccaggagga atgctttcgt
     9601 ggtctgcatg gaattgcctt ctcttgatgg ggaatgttcg aattaccggg ctgcgccgct
     9661 tgcgacggcg gcgtgcctgg cgatgctgcg gcccacctgc tgcgcggccg aaggcgggac
     9721 ttcacccagt accgtcacga tgttctcgcc catcggccgg acgaagaccg agagggcgcc
     9781 atcctcgttg gcggcttccg ccgtgcgcgg cgggttggac atgggctcga tgaatacgct
     9841 catcgtggcg atcccgtcgc tcaggacgag ttgcgtgacg ggatgcgcgc ggttggggag
     9901 cttgcgctgc atctcgccca cgaggcgaaa gccgggcggc ggcagcgcca cggaccatcc
     9961 cgtcgaggcg tggttcggct cgtcgggcgg ctggacgtcg cggcgccagt ccttgctctg
    10021 cggcaggaaa gtgctcttca cctcgccgcg cgagacgtca cgaccgacgc gcaggtcggt
    10081 gaaggcgtac tgttccagca cctgctgctt cgccccgagg gtcttcgcgc gcagcagcag
    10141 gccggagccg acctcggcgc aaaggcgctg cgcgtagcgc agcgcgtcct tcgggtcgag
    10201 caggatccac tggcagtcct ggcccaggac gcgctcgaca ttgccgagcg cgagcttgta
    10261 gttttccgcg atcgcctcca tcggcccgcc caccagcgag gggaagaatt tcgccgtcac
    10321 gcggcggtcg atgcgtatcg tcttcgcatc cggaaagaag cattgcatct ggtcgtcgtg
    10381 gcgcacgatc tcgcggcgcg ggccgtccag ggattcgatc ttctcgtgct cggaacccga
    10441 gacgaacacg tgcgtgatcc gcgaggtggc ggtgcgctcg ccctgcatgt gcatcacggt
    10501 gccggcgtag gtggtctgcc gcgccgaagt cgcggcgcgc tgcagccagg tgaccggatc
    10561 gctgcccggg tcggcgactg ccgccaggcc cgcgccaagc gccagcgacg cgagcgccgc
    10621 cgcctgccgc ctcatcggac ggccgcggcc tggttggcaa ccggccggta gaaatccgcg
    10681 ttgggaagct gtcgatggac caccacgtac tcgttcacgt tcttgagcgg cacgacgttg
    10741 tcggccacca ccggcggggg ggtggccggg gaggacagcg ccaccgccgg tcccgtgggg
    10801 gtgccgcgat cctgaaggcc gatccagccc accaccgcca cggtggcgac ggaggctgcc
    10861 gccgcgaagg cgatgcggcc ggcgctggcc gacacgcgcc gtctcggcgc aagcaccgtg
    10921 ggctcgccgc cgatggcttc catgatccgc cgggtggagg tgctggcaca ggtgccctcg
    10981 cccctcagcg cctcgccgat caggtggtac gtccgccact tgtgcctcag ttcctcgtcg
    11041 ccgcaacaat tgcggatggc gcgctcggcc tcttgggcac ccagttcgcc gtccatcaga
    11101 gaagaaagtt cctgcgtcat gatccgaccc cgaaattgcc cgggaggaag cctttttcgg
    11161 cccttgcccc gggaagtgtt gccgccctgc tgcctgttct caccatctgt ggtccttcga
    11221 cgtcccgagc tgcgggcgca gcctttcggc caccgcctcg cgggcacgga agatgcgcga
    11281 gcgcaccgtg ccgatcgggc aatccatgat cttcgcgatc tcctcgtagg agaggccgtc
    11341 gatctcgcgc agcgtgatcg ccgtgcgcaa ctcccccgga agggcatcga tcgcgtcgtt
    11401 caccgtcttc ccgatctgct tggacatcag caggttctcg ggagtgttga tgtcgcgcaa
    11461 tcccgcggca tcctcgaatc cttcggcctc ctcgctgtcg tattccgtcg aggtgggcgc
    11521 gcgccggccc tgcgcgatga ggtagttctt ggccgtgttg atgccgatgc ggtagagcca
    11581 cgtgtagaag gcgctgtcgc cccggaaatt cggcagcgcc cggtacgcct tcacgaaggc
    11641 ctcctgcgcc acgtcctcga tctcgttggg atcgcgaatg atgcgcgaaa gcaatcgctc
    11701 gagcttgcgc tggtacttga tgaccagcag ctcgaaggca cgcttttcgc ctcgctgggc
    11761 gcgttcgacc agttgctggt cgatttccct gtcgctcatc cgtccctgac tgggctgtct
    11821 tgtggatccg gaagccagtc ccatggccga cgccgccgtg ggcggggcga caaagggttt
    11881 gccggtttcc tgcggtatta taaacgtcag gtttttcatt accttgccgt gcgcaatccc
    11941 gcgactatgg ccaacagact ccccgccggg caaatagttc ctgcgggtcc gcgtcgccac
    12001 ccctctccca ggaaactttc ttgatcactt acgacgtcgt cgtcatcggc agcggccttg
    12061 ccggactgtc ggcagccctg cgcctcgcgc ccacccgcaa ggtggccctg gtcaccaagc
    12121 gcgaactgct ggacggcgcg tccagccacg cgcagggtgg catcgcggcg gtgctcgaca
    12181 gcgccgattc cctggaaaac cacatccgcg acacgcacgt ggccggcggc ggcctgtgcc
    12241 acgacgatgc ggtgcgcttc gtggtcgagc gcgggcgcca gtccatccag tggctcgtcg
    12301 accagggcgt gccgttcacg cgcgaggcgg gcggcacgga ggagcttcac ctccaccgcg
    12361 agggcggcca cagccaccgg cgcatcgtcc acgcggcgga cgcgacggga aacgccgtcc
    12421 agcgcaccct cctgcaacag gtgaaggcgc accccaacat cgagatcctc gaatggcatc
    12481 tcgccattga cctcatcacc ggccgcaagt tcggcgtggg cgacgaccgc tgctacgggg
    12541 cctacgtcct cgacctcaag gcgggcaagg tcaagaccct cgcggcgcgc tccaccatcc
    12601 tcgccacggg cggcatcggc aaggtctacc tgtacacgac caatcccgac accgccaccg
    12661 gcgacggcat cgccatgggc tggcgcgccg gctgcccggt ggcgaacatg gaattcatcc
    12721 agttccatcc cacctgcctc taccatccgc acgcgaagag cttcctcatc tccgaggcgg
    12781 tgcgtggcga gggcgggctg ctcaagctgc cgaacggcga gcgtttcatg ccacgccacg
    12841 acgagcgcgg cgagctcgcg ccccgcgacg tggtggcgcg cgccatcgac ttcgagatga
    12901 agaagcgcgg cctggattgc gtgtacctcg acatctcgca caagggcgag gcgttcgtcc
    12961 gcgagcattt ccccaacatc cacgccaagt gcctcgagtt cggcatcgac atcggcaagg
    13021 agccgatccc ggtcgtgccg gcggcccact attcctgcgg cggcctgcag gtggacctcg
    13081 ccggccgcac gccgctcgcg gggctctacg cgctgggcga agtggcgtgc accgggctgc
    13141 acggcgccaa ccgcctcgct tccaactccc tcctcgaatg cctcgtgttc tcggaggccg
    13201 cggtcgcgga catcctcgcc ggaacccccg tcaccgtccc gccgctgccg gcctgggacg
    13261 agagccgcgt caccgacgcc gacgaggagg tcgtgatcgc tcacaactgg gacgaactgc
    13321 gccgcagcat gtggagctac gtgggcatcg tgcgcaccaa caagcggctc gagcgcgcgc
    13381 tgcaccgcat ccagctcctc caggcggaaa tccacgagtt ctactccgcc ttccgcgtat
    13441 ccaacgacct gatcgagctg cgcaacctcg tggtcaccgc ggagctcgtc gtccgctcgg
    13501 cgctcgcgcg caaggaaagc cgcggcctgc atttcagcag cgactggccc gacatgcttc
    13561 ccgtggcgcg cgacaccgtg ctccacccgc ggcccgtcga aaagtgagcg ccctgcccct
    13621 cctgaccacg gcacaggttc gctcgctgga agaacgcgcc gccaggggac tggcggggcc
    13681 gacgctcatg caacgcgccg gtcgcgccac ggcggacgcc gcgctcgcgc tcgccgccga
    13741 caccggggaa ccgatcctcg tggtcgcagg gcccggcaac aacggcggag atgcgtgggt
    13801 ggcagccgcg cagcttcgcg agtccttcca ccgcgtcacg gtgctcgacg tcgcgggaac
    13861 ggagccgaag gcgcccgagg cacgcgaggc gcgcgcgcgc tttctcgccg gcggcgggca
    13921 ggcggttcgc caatggcccg cgataccgcg cccggcgctc gtgatcgacg gactgctcgg
    13981 catcggactc gcgcgcgacg tcgacggcga actggccgca ttggtcgcgc acatcaacga
    14041 ctgtggcgcg ccagtgctcg ccatcgacat cccgagcggc atcgcaagcg ataccggccg
    14101 cgtgcgcggg gtggccgtgc gggccgcgcg cacgatcacg ttcatcgcgc gcaaggtggg
    14161 cctgtacacg ggggatgcgc tcgaccactg cggcgagatc gtgttggacg atctcgggct
    14221 ggatgcggca ctgctcgcgg acactccagg gagcctcctc acgcccgaga gcgtccgcgg
    14281 ctggctcgcg ccgc
//
LOCUS       DASUEV010000327         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1242160, whole genome shotgun sequence.
ACCESSION   DASUEV010000327 DASUEV010000000
VERSION     DASUEV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242160"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1651
                     /locus_tag="VFV55_10370"
     CDS             173..1651
                     /locus_tag="VFV55_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4944748.1"
                     /translation="MSPDPPASPDSADRWREAVLTAGLAVFDWDIPAKRIYRSPEMLA
                     TWDYPDGPNDALDSARAVVHSDDYPAFRARMTEVEGGAIERATLEIRMRASDGAWRWV
                     RWRARVVRRGEDGRPLRVVGTVSDIDELWKGRDLARESQMRLQGIVDSAMDAIISIDS
                     EQRITLFNRAAERIFGYEAREALGHPLDMLIPARFHVEHHEHVKSFERTGVSSRPMMA
                     QRIVTALRKGGEEFPIDASISQNEIRNQRFFTVILRDVSLRERTRRELARAREDLREL
                     SVASRTAREQENSRISRELHDELGQNLTSLKMDLAWLEANCEPRNTKVMKALSGMRSV
                     LDSTVAATRRISADLRPLMLDDLGLLAALEWLAEETSRRHGFTIDLSVDEASGNLAEP
                     LASHIYRIVQESLTNAGRHAAAQHVRVTLRTIGPEIHLDVRDDGRGLAPVDLQKKGSF
                     GLVGIRERVYILAGSVEIRGDTGLGTEIHVRLPIPAAHEDGT"
     gene            1648..>2271
                     /locus_tag="VFV55_10375"
     CDS             1648..>2271
                     /locus_tag="VFV55_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4944749.1"
                     /translation="MTRVLIADDHAIVREGLKRILDGQEGIEIAGEATNGHETLERVR
                     EGGFDILMLDLSMPGKSGIELIKQVKVESPKLRVLVLTMHEEDQYAVRAIRAGASGYL
                     TKESAPAQLVSAIRRLAEGRLYISPNVAEQLALDVQPHGDEAPHRHLSDREFEVFLLL
                     VSGSGVSDIAAQLHLSVKTVSTHKTRILQKMSATSVADLVRYAIRHRI"
BASE COUNT          383 a          810 c          752 g          326 t
ORIGIN      
        1 tgccgccctc gaattcgctg cggcggtgga gtcgaagctg ggctggcggg tccacgtacc
       61 cggtcccggc gagacggcgg actgctgaaa gcggggcacg ccgcacggaa ccccctggtc
      121 ctgctatcct ttcctcgcga atgtaacctt tcccgtaact cccctcccct tcatgtcccc
      181 agacccaccc gcgtccccgg acagcgccga tcgctggcgc gaagccgttc tcaccgcggg
      241 cctcgcggtg ttcgactggg acatacccgc gaagcggatt taccggtcgc cggagatgct
      301 cgccacctgg gactatcccg acggccccaa cgacgccctg gatagcgcgc gggcggtcgt
      361 gcactccgac gactaccccg ccttccgggc acgcatgacg gaagtggaag ggggggcgat
      421 cgagcgcgcc accctcgaaa tccgcatgcg cgcgagcgac ggggcatggc ggtgggtgcg
      481 ctggcgcgcg cgcgtggtgc gccgcggcga ggacggccgg cccttgcgcg tcgtggggac
      541 ggtgagcgac atcgacgagc tctggaaggg ccgcgacctc gcccgcgaaa gccagatgcg
      601 cctgcagggc atcgtcgatt cggccatgga tgcgatcatc tccatcgaca gcgagcagcg
      661 catcacgctg ttcaatcgcg ccgccgagcg catcttcggc tacgaggcgc gcgaggcgct
      721 cggtcacccg ctcgacatgc tcatcccggc ccgcttccat gtcgagcacc acgagcacgt
      781 caagtccttc gagcgcaccg gcgtctcgag ccggccgatg atggcgcagc gcatcgtcac
      841 cgcgctgcgg aagggaggcg aggaattccc gatcgacgcc tcgatctcgc agaacgagat
      901 ccgcaaccag cgcttcttca ccgtgatcct tcgcgacgtg tcgttgcgcg agcgcacgcg
      961 ccgcgaactt gcgcgggccc gcgaggacct gcgcgagctc tcggtcgcct cgcgcacggc
     1021 tcgggagcag gaaaacagcc gcatctcccg cgagctccac gacgagctgg gccagaacct
     1081 cacctcgctc aagatggacc tcgcgtggct ggaggccaac tgcgaaccgc gcaacacgaa
     1141 ggtgatgaag gcgctctccg ggatgcgttc ggtcctcgac agcacggtgg ccgccacgag
     1201 gcgcatttcc gcggacctgc gcccgctcat gctcgacgac ctgggcctgc tggccgcgct
     1261 cgaatggctg gccgaggaaa cgtcgcgccg gcacggcttc accatcgacc tgtccgtcga
     1321 cgaggcgagc ggcaacctgg ccgagcccct cgcctcgcac atctaccgca tcgtccagga
     1381 gagcctcacc aacgccggcc gccacgcggc ggcgcagcac gttcgcgtga cgctgcgcac
     1441 gatcgggccc gagattcacc tcgacgtgcg ggacgacggc cgcgggctgg cccccgtcga
     1501 cctgcagaag aagggctcct tcggactggt cgggatccgc gagcgggtct atatcctcgc
     1561 gggctccgtc gagatccgcg gagacaccgg gctgggcacg gagatccacg tgcgcctgcc
     1621 gattcccgcg gcccacgagg acgggacatg acccgcgttc tcatcgccga tgaccatgcg
     1681 atcgtccgcg agggcctcaa gcgcatcctc gacgggcagg aagggatcga gatcgcgggc
     1741 gaggcgacca acgggcacga gaccctcgag cgcgtgcggg agggcggatt cgacatcctg
     1801 atgctcgacc tttcgatgcc gggcaagagc ggcatcgagc tcatcaagca ggtgaaggtc
     1861 gaaagcccga agctgcgcgt gctcgtcctc accatgcacg aggaagacca gtacgcggta
     1921 cgggccatcc gtgccggggc gtcgggttat ctcaccaagg aaagcgcgcc ggcccagctc
     1981 gtttcggcga tccggcgtct ggccgagggc cggctctaca tcagccccaa cgtggccgag
     2041 caactggcgc tcgacgtcca gccccatggc gacgaggcgc cgcaccgcca cctctccgac
     2101 cgcgaattcg aggtgttcct gctcctggtg agcggcagcg gcgtctcgga catcgcggcc
     2161 cagctgcacc tgagcgtgaa gaccgtcagc acgcacaaga cgcggatcct gcagaagatg
     2221 agtgccacgt ccgtcgcgga cctcgtccgc tacgcgatcc ggcaccgcat c
//
LOCUS       DASUEV010000328         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_190702, whole genome shotgun sequence.
ACCESSION   DASUEV010000328 DASUEV010000000
VERSION     DASUEV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190702"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="VFV55_10380"
     CDS             complement(<1..1991)
                     /locus_tag="VFV55_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4944750.1"
                     /translation="MKLPEICIQRPVFATVLSVIVFLVGLISYSRLSVREYPRIDEPV
                     VSVETVYQGASAEVIESQITKVLEDSLSGIEGVELMTSSSRSERSQITVRFRLTRDPD
                     SAAADVRDKVARVRGRLPDAVDEPVIAKVEADSFPILWMAVSAPGKDALEVSDYVARY
                     IKPRLSTLPGAADVRIFGERKVSMRIWVDRAKLAAYRLTVQDLEDALRRQNVEIPAGR
                     IESDKREFSVLSLTDLQTPQQFGAIVVREVSGYPVRVRDLGRVEVGPLDERIITRYMG
                     GTAVSLGVIKQAVANPLELSLAVRKEIAEINKTLDGMKLELMYDSSVFIDQSIKAVFA
                     TIAEAIVLVALVIFMFLRNLRATIIPLVTIPVSLVGACAFMYAFGFSVNTLTLLALVL
                     AIGLVVDDAIVVLENVYRNLENGMSRLDAALVGSREIAFAVLAMTLTLAAVYAPLAFA
                     TGRTGRLFIEFALALAGAVLVSGFVALTLSPMMCSKLLRHETKHSAIYNAIEGFFHAL
                     TAHYRRVLGLALHHRVWVVAGAVLVACASYVLFKNLRTELSPVEDRGVVFSFVSAQEG
                     STARYTADNLKPIEDIYRTIPEMQRFNALAGFPTVSDGIAILRLKPWEERTRKQQEIT
                     QELAPKFAQIPGVRAFPTNPPSLGQSPRNRPVEFVVMSSA"
     gene            complement(2005..>2277)
                     /locus_tag="VFV55_10385"
     CDS             complement(2005..>2277)
                     /locus_tag="VFV55_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944751.1"
                     /translation="AIPEESIFPVGDDKYVFKVVDGRAQRQKVETGQRRDGRVEILAG
                     IGAQDIVVSAGVVKLRDGVPVRVASPAVAQPPVGKADSAPSGKGNS"
BASE COUNT          403 a          738 c          794 g          342 t
ORIGIN      
        1 gcggagctca tgacgacgaa ctcgacgggg cggttgcgcg gcgactgccc gagggacggc
       61 gggttggtcg ggaaggcgcg cacgcccggg atctgggcga acttcggggc cagctcctgc
      121 gtgatctcct gctgcttgcg cgtgcgctct tcccagggct tcagtcgcag gatcgcgatg
      181 ccgtccgaga cggtggggaa cccggccagc gcattgaagc gctgcatctc ggggatggtg
      241 cggtagatgt cctcgatggg cttgaggttg tccgcggtgt accgggcggt cgagccctcc
      301 tgtgccgaca cgaagctgaa cacgacgccg cgatcctcca cgggtgacag ctccgtgcgc
      361 aggttcttga agaggacgta gctcgcgcag gccacgagga cggccccggc caccacccac
      421 acgcggtggt gcaatgccag gccgagcacg cgccggtaat gggcggtgag cgcatggaag
      481 aagccctcga tcgcattgta gatggcgctg tgcttcgtct cgtggcgaag cagcttcgaa
      541 cacatcatgg gcgacagcgt gagtgccacg aaccccgaaa ccagcaccgc gcccgcgaga
      601 gcgagggcga actcgatgaa gagccgcccc gtgcgaccgg ttgcgaacgc gaggggagcg
      661 tagaccgcgg cgagcgtcag ggtcatcgcg agcaccgcga aggcgatctc gcgcgagccc
      721 acgagggcag cgtccaggcg cgacatgccg ttctcgagat tgcggtagac gttctccagc
      781 accacgatcg cgtcgtccac caccaggccg atggcgagca cgagggcgag cagggtgagg
      841 gtgttgacgc tgaagccgaa ggcgtacatg aacgcgcacg ccccgacgag cgagacggga
      901 atggtgacca gcggaatgat ggtcgcgcgc aggttgcgca ggaacatgaa gatgacgagc
      961 gccaccagca cgatggcctc ggcgatcgtg gcgaacaccg ccttgatcga ctggtcgatg
     1021 aacaccgacg agtcgtacat gagctcgagc ttcatgccgt caagcgtctt gttgatctcg
     1081 gcaatctcct ttcgcaccgc cagcgaaagc tcgagcggat tggccaccgc ctgcttgatc
     1141 acgcccaggc tcaccgcggt gccgcccatg tagcgcgtga tgatgcgctc gtcgagggga
     1201 cccacttcga cgcgcccgag gtcgcgaacg cgaaccgggt acccgctcac ctcgcggacg
     1261 acgatggccc cgaactgctg gggcgtctgc aggtcggtga gcgagagcac cgagaactcg
     1321 cgcttgtcgc tttcgattcg gccggcagga atctccacgt tctggcgccg cagcgcgtcc
     1381 tcgagatcct gcacggtgag gcggtaggcg gcgagcttgg cgcggtccac ccagatgcgc
     1441 atcgacacct tgcgctcgcc gaagatccgc acgtcggcgg cgccgggcag cgtggaaagg
     1501 cgtggcttga tgtagcgcgc gacgtagtcg gaaacctcga gcgcgtcctt gcccggtgcc
     1561 gaaaccgcca tccacaggat cgggaaggaa tccgcctcca ccttggctat gaccggctcg
     1621 tcgacggcat ccggcagccg cccgcgcacg cgcgccacct tgtcgcgcac gtcggccgcg
     1681 gcggaatcgg gatcgcgggt gagccggaag cgcaccgtga tctgcgagcg ctccgagcgc
     1741 gaggacgagg tcatgagttc cacgccctcg atgccggaca gcgagtcctc gagcaccttg
     1801 gtgatctggg attcgatgac ctccgcgctc gcgccctggt agacggtctc cacggagacc
     1861 accggctcgt cgatgcgcgg atattcgcgc acggacaggc gcgaatagga gatgagcccc
     1921 acgaggaaca cgatgacgga gagcaccgtc gcgaagaccg ggcgctggat gcagatctcg
     1981 ggcagtttca tcgcggcatg cgggtcagga attgcccttg ccggacgggg cggagtcggc
     2041 cttgccgacg ggcggctgcg cgacggccgg gctcgccacg cgaacaggca cgccgtcgcg
     2101 aagcttcact acccccgcgg agaccacgat gtcctgcgcg ccgatcccgg cgaggatctc
     2161 gacacggccg tcgcggcgct ggccggtctc caccttctgg cgctgggcgc ggccgtccac
     2221 gaccttgaag acgtacttgt cgtcgcccac cgggaagatc gattcctcgg gaatcgc
//
LOCUS       DASUEV010000329         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1242471, whole genome shotgun sequence.
ACCESSION   DASUEV010000329 DASUEV010000000
VERSION     DASUEV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242471"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFV55_10390"
     CDS             <1..301
                     /locus_tag="VFV55_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX4944752.1"
                     /translation="EADADGYLKEPDFSEEAVQVIAAADGIALTEDHWKVVNYLRDEF
                     RDKGHTPNFRNMMKDLQEALPGCDSKSLYDLFPLGPAKQGCKVAGLPQPYGKGGY"
     gene            489..788
                     /locus_tag="VFV55_10395"
     CDS             489..788
                     /locus_tag="VFV55_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944753.1"
                     /translation="MKYDISFVISVLAILVMFYCLWLVLSLRKNIPGGVVGGTWKTLT
                     SLVILFTLGYLVTPFFSLLPPILANVIVSLIFLFGAIYVVITVKLIYRIIEELAG"
     gene            793..1272
                     /locus_tag="VFV55_10400"
     CDS             793..1272
                     /locus_tag="VFV55_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944754.1"
                     /translation="MDPHAILAKTPLGVEVLSHRDHDLPRTLRHALILVDDHSTVAGL
                     EQKCAMIPEFAAALRELVARGLVAPRGDGVTVAPAAAAPAGATELPIVQALVGMAESI
                     LGERSGKVVKKLEGAGATREELFATVDACFKLIRLTIDEAQAEEFRRAARELLSRGD"
     gene            complement(1370..2398)
                     /locus_tag="VFV55_10405"
     CDS             complement(1370..2398)
                     /locus_tag="VFV55_10405"
                     /inference="COORDINATES: protein motif:HMM:NF044557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944755.1"
                     /translation="MSLPFAPLLAAVQSNCHLSDARHAQDLTLCNYLLAMREYYRWEH
                     DLPPESMPPRADVMRWIAEREALWESLAESEWTNVPLAAGDADPFDVNTVNAELVPAG
                     LLYGAGRGVFGKPHFFLARLARHERRDGIEILEAGCEYVRDLEAAPAVLQGETVVLRR
                     EAFERWLWLRAETWEAHESEGAMGAALDAYGYGKERGRALGRMAEAERETLILHEMGE
                     LAAGRLLGEGWERFMDALDDRRVEITARAVRDLLADTLVTLPTLLDREAVPSLHFWFA
                     TFSGLRRDLFPRLSSAQAVWSTGGGPEALRDALEAGRAHWLRVARELAADPEAARRFA
                     LEPGLITL"
     gene            complement(2405..2629)
                     /locus_tag="VFV55_10410"
     CDS             complement(2405..2629)
                     /locus_tag="VFV55_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944756.1"
                     /translation="MPYYIYKVFPFHRLEKVTETDGFPQASAQAKAMRKDPALPADCK
                     VKVIFAENELGAETLLTEVREPHPRFEDDD"
     gene            complement(2645..3022)
                     /locus_tag="VFV55_10415"
     CDS             complement(2645..3022)
                     /locus_tag="VFV55_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX4944757.1"
                     /translation="MTGSAKPLRRTIPIAPASDDDHGACSGSESFALLVLGDSMAPEF
                     AEGDVIVIEPEGLATDGSFVLARHDGEWIFRQLVAAGEGWRLRPLNPHYPTVALGDLA
                     AVRGVVIQKTTPGNRKSTTRYGE"
     gene            complement(3031..>3388)
                     /locus_tag="VFV55_10420"
     CDS             complement(3031..>3388)
                     /locus_tag="VFV55_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin oxidoreductase"
                     /protein_id="HEX4944758.1"
                     /translation="FAACDLGFAPVKSLVEYALSADAAASYTVAWLATRADGHYLANQ
                     CRAWAEALDDFTWLPVADEDPAAGAARLVEALRTTADLSRAEVYIAGPEEFVRSAEFE
                     FRAAGVAEGAIAMLAL"
BASE COUNT          531 a         1127 c         1187 g          543 t
ORIGIN      
        1 ggaagcggac gcggacggct acctcaagga gccggatttc agtgaggagg cggtgcaggt
       61 gatcgcggcc gcggacggga tcgcgcttac cgaggaccac tggaaggtgg tgaactacct
      121 gcgcgacgaa ttccgcgaca aggggcacac gcccaacttc cgcaacatga tgaaggacct
      181 gcaggaggct ctccccggct gcgattcgaa gtcgctttac gacctctttc cgctgggccc
      241 ggccaagcag ggctgcaagg tggcggggct gccgcagccc tacggcaagg gcgggtactg
      301 aactgccggc ggcgccgcaa gtgacggcag gttgatctgg cgcaaggccg aaaatggcat
      361 tcgatacacc ctgagcgcag tcgatgccgc gggcgtgggc ggctgcgcgc cgacccgacc
      421 catgatccgt cctcgcccga gattcgcccg ttcgattttc cgatgaacac acgcagggag
      481 tacaggcagt gaaatacgac atcagcttcg tgatcagcgt tctggccatc ctggtgatgt
      541 tctattgcct gtggctggtg ctctcgcttc gcaagaacat ccccgggggc gtcgtcggcg
      601 gcacctggaa gacgctcact tccctggtga tcctcttcac gctgggctac ctcgtaacgc
      661 cgttcttcag cctgctgccg ccgatcctcg cgaacgtgat cgtctcgctc atcttcctgt
      721 tcggcgcgat ctacgtcgtg atcacggtga agctgatcta ccgcatcatc gaggaactcg
      781 cggggtgagc ttatggaccc gcatgccatc cttgccaaga cgccgctggg cgtcgaggtc
      841 ctgagccacc gggaccatga cttgccgcgg acgcttcgcc acgcattgat cctcgtcgac
      901 gaccattcga cggtcgccgg gctggaacag aagtgcgcga tgatcccgga gttcgcggcc
      961 gcattgcgtg agttggtcgc gagggggctc gtcgcgcccc gcggcgacgg tgtgaccgtg
     1021 gcgccggcgg cggcagcgcc ggcgggcgcg acggagttgc ccattgtcca ggcactcgtg
     1081 ggcatggccg agagcatcct cggggagcgt tcgggcaagg tcgtgaagaa gctcgagggc
     1141 gcaggcgcga cgcgcgagga actcttcgcg accgtcgacg cgtgcttcaa gcttatccgc
     1201 cttaccatcg acgaggccca ggcggaggaa ttccgccgtg ccgcaagaga gttgctttcc
     1261 cgcggcgatt gagcaagtcg ccgcggtacg ccgcgttttc ggtgtcagac acgtctgcgt
     1321 ggcgcggcgg cccggtgtct ggcaccgaaa gtaatctgac cctttgccgt catagggtga
     1381 tcaagccggg ctccagcgcg aagcgcctgg cagcttccgg gtcggccgcg agttcccgcg
     1441 ccacgcgcag ccagtgcgcc cggcccgcct cgagggcatc gcgaagcgct tccggcccgc
     1501 cgcccgtgct ccacacggcc tgcgccgatg aaagccgggg aaagaggtcc cggcgcaggc
     1561 cggaaaaggt cgcgaaccag aagtgcagcg acggcaccgc ctcgcgatcg aggagggtgg
     1621 gcagcgtgac cagggtgtcg gcgagcaggt cgcgcaccgc gcgcgccgtg atctccacgc
     1681 gccggtcgtc cagcgcgtcc atgaaccgtt cccagccctc gcccaggagc cggcccgcgg
     1741 cgagctcgcc catctcgtgc aggatgagcg tttcgcgctc cgcctcggcc atgcgcccca
     1801 gggcgcggcc gcgttccttc ccgtatccgt aggcgtcgag ggcagcgccc atggcgcctt
     1861 cgctctcgtg cgcctcccag gtctccgcgc gcagccaaag ccagcgctcg aaggcctcgc
     1921 ggcgcaacac gaccgtctcg ccctggagga cggccggggc ggcctcgagg tcgcggacat
     1981 actcgcatcc ggcctcgagg atctcgatgc cgtcgcgccg ctcgtgccgc gcaaggcgcg
     2041 caaggaagaa atgcggcttg ccgaacacgc cgcggcccgc gccgtacagg agccctgcgg
     2101 ggacgagctc ggcattcacg gtgttcacgt cgaacgggtc ggcatctccc gcggccaggg
     2161 ggacattggt ccattcggac tccgccagcg attcccacag cgcttcgcgc tccgcgatcc
     2221 agcgcatcac gtcggcgcgc ggcggcatcg actcgggcgg caggtcgtgc tcccaacggt
     2281 agtactcgcg catggcgagc aggtagttgc agagcgtgag gtcctgcgcg tgtcgcgcat
     2341 ccgacaggtg gcagttggat tgcacggccg ccagcagggg cgcgaaagga aggctcatgg
     2401 cgcgtcagtc gtcgtcttcg aagcgcgggt gcggctcgcg cacctcggtg agcagcgtct
     2461 cggcgcccag ctcgttctcg gcgaagatca ccttcacctt gcagtcggcg ggcagcgccg
     2521 gatccttgcg catcgccttg gcctgcgcgg aggcctgcgg aaacccgtcg gtctcggtca
     2581 ccttctcgag ccggtggaaa gggaagacct tgtagatgta gtaaggcatg ggtcgcggac
     2641 cttcctattc gccatagcgg gtggtcgact tgcggttgcc gggcgtcgtc ttctggatca
     2701 ccacgccgcg cacggcggcg aggtcgccca gggcgacggt tggataatga gggttcaggg
     2761 ggcgcagccg ccagccttcg ccggcggcga cgagctggcg gaagatccac tcgccgtcat
     2821 ggcgggcgag cacgaagctg ccgtcggtgg cgagtccctc gggctcgatc acgatgacgt
     2881 cgccctcggc gaattcgggc gccatcgagt cgcccagcac gaggagcgcg aaggactccg
     2941 acccgctgca ggcaccgtgg tcgtcgtcgc tcgcgggggc gatgggaatg gtgcggcgca
     3001 ggggcttggc ggatccggtc atgtccggtc tcacagggcg agcattgcga tggcgccttc
     3061 ggccacgccg gcggcccgga actcgaactc ggccgaccgc acgaattcct cgggccccgc
     3121 gatgtagacc tccgctcgcg aaaggtcggc agtggtgcgc aatgcttcca cgagccgcgc
     3181 cgcgccggcc gccggatcct cgtcggcaac gggcagccag gtgaagtcgt cgagcgcctc
     3241 ggcccatgcg cggcactggt tggcgaggta gtggccgtcg gccctcgtgg cgagccaggc
     3301 gacggtgtag gacgcggcgg cgtccgccga gagcgcgtac tcgacgaggc tcttcaccgg
     3361 ggcgaatccc aggtcgcacg cggcgaac
//
LOCUS       DASUEV010000330         8170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_787622, whole genome shotgun sequence.
ACCESSION   DASUEV010000330 DASUEV010000000
VERSION     DASUEV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787622"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..830)
                     /locus_tag="VFV55_10425"
     CDS             complement(87..830)
                     /locus_tag="VFV55_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4944759.1"
                     /translation="MFATMEALSASPDPSASPRPRVLLIEDNVRLAASIRDYLQKHQV
                     EVHVEGDGLAAAARIERLHPDLVILDLMLPGKDGLSICRELRRTDRTPVLMLTAKGED
                     IDQVLGLEMGADDYVVKPVEPRVLLARIEAILRRARPAQGSPKGARLVAGRLEIDAGR
                     RSAAIAGRPIELTTGDFDILWLLASRQGSVVTRDEILRVVRGIDYDGLDRSIDARICR
                     LRRKLNEAGGAESMIKTVRLRGYLFAGDA"
     gene            1047..1454
                     /locus_tag="VFV55_10430"
     CDS             1047..1454
                     /locus_tag="VFV55_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HEX4944760.1"
                     /translation="MRTPAILATTLVLAAAMPALAETPAQFRDAFAAEAKRENPAFAG
                     LSADRGRQFFSATHGKDWSCSTCHTANPAAAGKHATTGKAITPLAPSANPERFTSAAK
                     VDKWFKRNCNDVAGRACTAQEKGDILAWLLATR"
     gene            1463..1960
                     /locus_tag="VFV55_10435"
     CDS             1463..1960
                     /locus_tag="VFV55_10435"
                     /inference="COORDINATES: protein motif:HMM:NF021165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HEX4944761.1"
                     /translation="MNPVRNALRLTSLYVIAAAATAHALSAHAGKHVYPIDNAAYRQE
                     CASCHVAYPPQLLPAQSWRAVMGGLDKHFGTDASLEPAVRADILAYLERTSSGRDFWS
                     LGQPVLRVTQAPWFEKEHRKEIAAGTAQRADVKSIANCGACHKGAENGDYGKAGRQVP
                     GGRAR"
     gene            1957..2535
                     /locus_tag="VFV55_10440"
     CDS             1957..2535
                     /locus_tag="VFV55_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX4944762.1"
                     /translation="MSALPRTSDAGMPRTNSGTVVVWDILTRAFHWSFAACFAGAWLT
                     AESERFRDVHVNLGYTMLGLIVFRVLWGLVGPKHARFANFVRGPAAVLKYLRSLLGPR
                     PEHHLGHNPAGAIAIVLLLVLGLASIASGWAIYNDLGGEWLEESHEFLAGAMLAAVGV
                     HLAGVAISSLLHRENLVGAMLTGRKNAPGRRP"
     gene            2558..3229
                     /locus_tag="VFV55_10445"
     CDS             2558..3229
                     /locus_tag="VFV55_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4944763.1"
                     /translation="MGVRVLVVEDDALLGDAIQAGLRQKGFVADWVRDGAAADLALES
                     GDHAAVVLDIGLPRRSGLEVLARLRARKNPVPVLILTARDTVEDRIAGLDAGADDYLV
                     KPFDLGELAARLRALTRRASGSTSAAITVGALCLDPATREVSWRGEARILSRKEFSLL
                     HAFMLNAGKVMTREQLEDKLYGWGEEIESNAMEVHLHHLRKKIFPGVVQTLRGVGYVL
                     PRDLS"
     gene            3226..4563
                     /locus_tag="VFV55_10450"
     CDS             3226..4563
                     /locus_tag="VFV55_10450"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4944764.1"
                     /translation="MTTVRGSVRGRLIAILLTGLAVVWLGATVATVIEADHEVEEVFD
                     AHLAQKASLLALRVGEEMEEIDTEHAPVLHKYAKPLSFQVWKHGTKLLLHSADAPDMR
                     FSPDESGFSVVTFDDRDWRVFSLWDAKHEHLVQVRDAIKERRHVMVDIMEALAMPLAV
                     ALPLFALLVWFAVGRALRPLARVSDEISRRDPKYLAPLEGEVPSEIAPLVGRLNALLA
                     RVQSSLDGERRFTSDAAHELRTPLAALRAQLQVAQGAADAAGRDRAIANALAAGERAT
                     HLVEQLLTLARLEHSAWQAVAAPFDLHRVAAEAIGQRAPQALARRIELSLEGEAGAMA
                     MGHAGLAAIAVGNLLDNAIRYSPADTAVTVAVARDGGRAILSVRDEGPGIPAGRREDA
                     LRRFTRPEDSGTEGSGLGLSIVARIAELHGAALQLGDAAGGKGLVATLRFPAA"
     gene            4655..6268
                     /locus_tag="VFV55_10455"
     CDS             4655..6268
                     /locus_tag="VFV55_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX4944765.1"
                     /translation="MTIAQQVARRRTFAIISHPDAGKTTLTEKLLLFAGAIQIAGSVK
                     ARKASRHATSDWMEIEKQRGISVASSVMQIEYRGCVVNLLDTPGHQDFSEDTYRVLTA
                     VDAALMVIDAANGVEAQTLRLLQVCRARNTPILTFINKMDREVRNPVDLVDEIERTLG
                     MAAVPFTWPVGMGKTFHGVYDLRNARMRVFRPGADRLNDEGDEIVADIDDAGVCARFG
                     AELVQAKQEIELVQGASPDFDHAAFLAGRQTPVFFGSAINNFGVREVLDALADLAPPP
                     GPRAAIQREVEPREGKFTGVVFKIQANMDPAHRDRIAFVRVCSGQFERGMRLTVARNG
                     KVIRPGSVVSFLSQRRELLEFAFPGDIIGIPNHGTLQLGDTLSEGEVLQFTGLPFFAP
                     EIFQAVEIADPMRSKQLKLGLAQLGEEGAIQVFHPHHGGALLLGAVGQLQFEVVAHRL
                     QHEYGVPVRMVPSKFRLARWVTSDDPKELARFIEANGHRVAYDAVDAPTFLATANAEL
                     VVTQDLWKGIQFHALREHAGLVFQARLEG"
     gene            6344..>8170
                     /locus_tag="VFV55_10460"
     CDS             6344..>8170
                     /locus_tag="VFV55_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX4944766.1"
                     /translation="MSFAHRLSLKARVVLLMLAIVVPFGGVLAGYIANEYAEARTNAY
                     RHAEGVAAISAARLSLKLGDLEAILSRLAERPSIRALDPERCDPIIDEYVRLHPEFTT
                     LALRDRDGNIACSFLPGPIAKLDPGKFPWFGEGLRNAKFTAGNAFLGHQTGKWVSVLT
                     QPVRDDSGRTSGLLVMAVELLPLSRQIFEGVPENLLMAVVDREGKVLLRSRDHEARVG
                     KAIPAHFVEQTRGRDQGNLAERGTDGVRRLYAFTTIPGTGWRVFAALDEKEALATYRR
                     DLLAGVALGIPLALLVLASAWWVGSAIANPIRSLARTAERVAEGDTNARAPIDGPAEI
                     RSVARQFNRMLDSFERHREERAALVGHFGQLAKVAPEIILLIDPDGRIVEANDAAAAA
                     YGYGVDELRGMHILDLRTPVDAALLAQQLQAADSPGGVLFETFHRRKDGSTFPVEVSA
                     RSLEIGGKLYRQSFVRDITARRQAEDAIRESEVRFRTVVESAPDGIFIQTGGRFAWLN
                     REAMRIFGIAGMEAARGQPVAERFHPDSRELARARIRLVNEERRSVHLRGRGRSARVR
                     ARHHRPQARPGGTAQERVPLPHAGGGRADHRRPGVRPRAPGGL"
BASE COUNT         1260 a         2924 c         2757 g         1229 t
ORIGIN      
        1 gcaagcgtct ccccacatgc aaccatcggc tccggccgtg cctgctgcaa ccgtcgcatc
       61 cgcaccggcc gcaccgtcat gccttcctac gcgtcgcctg cgaacaggta gccacgcagc
      121 ctcaccgtct tgatcatcga ctccgccccg cctgcttcgt tgagcttgcg gcgcaggcgg
      181 cagatgcgcg cgtcgatgga gcggtcgagc ccgtcgtagt cgatgccgcg caccacgcgc
      241 aggatctcgt cgcgggtgac gaccgagccc tggcgcgagg cgagcagcca caggatgtcg
      301 aagtcgcccg tggtgagctc gatcgggcgg ccggcgatgg cggcgctgcg cctccccgcg
      361 tcgatctcga gccggcccgc gaccagcctc gcacccttgg gcgaaccctg tgcggggcgg
      421 gcgcggcgca ggatcgcctc gatccgcgcc agcagcacgc gcggctccac gggcttcacc
      481 acgtagtcgt cggcgcccat ctcaagcccc agcacctggt cgatgtcctc gcccttggcg
      541 gtgagcatga gcaccggcgt gcggtcggtc cggcgcagct cccggcagat ggaaagcccg
      601 tccttgcccg gaagcatgag gtccaggatc accaggtcgg ggtgcaggcg ctcgatgcgc
      661 gcggcggccg cgagcccgtc tccctccacg tgcacctcca cctggtgctt ctgcaggtaa
      721 tcgcggatgg aggccgcgag gcgcacgttg tcctcgatga gcagcactcg cgggcgcggg
      781 ctggcggagg ggtctggcga ggcggaaagg gcttccatgg tggcgaacat ggggcgtctc
      841 gaacggtggt gggaaagaat actccagccc ggcccggctc cccttcgccg cgcacatcgg
      901 gcaccgggct cgtgtccccg cgaaaatagg gactgtccct aattttcccg ctcgcgtgcc
      961 cggaactcgc tgcggggacc ttaaggaggt gttaaggcgg ctcgcgcagg atgcacccat
     1021 agcaatcgac cccgaaggag acaaccgtgc gtactcccgc catcctggcc accaccctcg
     1081 tcctcgcggc agccatgcct gccctcgccg aaacgccggc gcaattccgc gacgccttcg
     1141 ccgccgaggc gaagcgggaa aatcccgcct ttgccggcct ttccgccgat cgtggccgcc
     1201 agttcttctc cgccacgcac gggaaggact ggagctgctc gacgtgccat acggcgaacc
     1261 cggccgccgc cggcaagcac gccaccaccg gcaaggccat cacgccgctg gccccgtccg
     1321 ccaaccccga gcgattcacc agcgccgcca aggtggacaa gtggttcaag cgcaactgca
     1381 acgacgtcgc aggccgggcc tgcaccgcgc aggagaaggg cgacatcctc gcctggctcc
     1441 tcgccacccg ataggagcaa ccatgaatcc cgtgcgaaac gcccttcgcc tgacttccct
     1501 ttacgtgatc gccgccgctg ccacggcgca tgcgctctcg gcccacgccg ggaagcacgt
     1561 ctaccccatc gacaacgccg cctaccgtca ggaatgcgct tcctgccatg tcgcctaccc
     1621 gccgcagctc ctgccggcgc agtcgtggcg cgccgtgatg ggcggcctcg acaagcactt
     1681 cggcaccgac gcgagcctcg agccggccgt gcgcgccgac atactcgcct acctcgagag
     1741 gacctccagc gggcgcgact tctggtcgct cggccagccc gtgctgcgcg tgacgcaggc
     1801 cccgtggttc gaaaaggagc atcgcaagga gatcgcggcg ggcaccgcgc agcgcgccga
     1861 cgtgaagtcg atcgccaact gcggcgcctg tcacaagggc gcagagaacg gcgactacgg
     1921 caaggcgggc cgccaggttc ccggagggcg tgcacgatga gcgccctgcc tcgcaccagc
     1981 gacgcgggaa tgccgcgcac gaacagcggc acggtcgtgg tctgggacat cctgacgcgc
     2041 gccttccact ggtccttcgc cgcgtgcttc gccggcgcct ggctcaccgc ggaaagcgaa
     2101 cgcttccgcg acgtccacgt gaacctgggc tacaccatgc tcggcctcat cgtcttccgc
     2161 gtgctctggg ggctcgtggg cccgaaacac gcgcgcttcg cgaacttcgt tcgcggcccc
     2221 gccgccgtcc tgaaatacct ccgctccctc ctcggcccgc gccccgagca ccacctgggc
     2281 cacaacccgg ccggggccat cgcgatcgtg ctgctcctgg tactgggcct cgcgtccatt
     2341 gcctcgggat gggcgatata caacgatctc ggcggcgagt ggctggagga atcgcacgag
     2401 ttcctggccg gggcgatgct ggccgcggtc ggcgtgcacc tcgcgggcgt ggcgatctcg
     2461 agccttctgc accgcgagaa cctcgttggc gcgatgctga ccgggcgcaa gaacgcgccg
     2521 ggccgcaggc cttaaactcc cccttcacga caggaggatg ggcgtgaggg tcttggtcgt
     2581 cgaggatgac gcgctgctgg gagacgcgat ccaggcgggc ttgcggcaga aggggttcgt
     2641 cgccgactgg gtgcgcgacg gtgccgcggc ggacctcgcc ctcgaatcgg gcgaccacgc
     2701 cgccgtggtc ctcgatatcg gcctgccgcg ccgctccggg ctcgaggtgc tggcgcggct
     2761 gcgcgcgcgc aagaacccgg tgcccgtcct catcctcacc gcgcgcgaca ccgtcgagga
     2821 ccgcatcgcc ggcctcgacg cgggcgccga cgactatctc gtgaagccct tcgacctcgg
     2881 cgagctcgcg gcgcggctgc gcgcgctcac gcgccgcgcg agcggaagca cttcggcggc
     2941 gatcaccgta ggcgcgctct gcctcgaccc cgccacgcgc gaagtctcct ggcggggcga
     3001 ggcgaggatc ctctcgcgca aggagttctc gctcctgcac gcgttcatgc tcaacgccgg
     3061 gaaggtgatg acgcgcgagc agctcgagga caagctctac ggctggggcg aggagatcga
     3121 atcgaacgcg atggaggtgc acctgcacca cttgcgcaag aagatctttc cgggcgtcgt
     3181 gcagacgctg cgcggcgtgg gctacgtgct gcccagggac ctctcgtgac aaccgtgcgc
     3241 ggcagcgtgc gcggacggct catcgcgatc ctgctgaccg gcctggccgt cgtatggctc
     3301 ggtgccacgg tggccacggt catcgaggcc gaccacgaag tggaggaagt cttcgacgcg
     3361 cacctcgcgc agaaggcctc gctcctggcg ctgcgcgtcg gcgaggagat ggaagagatc
     3421 gacaccgagc acgcgcccgt gctgcacaag tacgcgaagc cgctttcctt ccaggtctgg
     3481 aagcacggca cgaagctcct gctgcattcc gcggacgcgc ccgacatgcg cttctccccc
     3541 gacgagtccg gcttctccgt cgtcaccttc gacgatcgcg attggcgcgt gttcagcctg
     3601 tgggatgcga agcacgagca cctggtgcag gtgcgcgatg cgatcaagga acggcgccat
     3661 gtaatggtcg atatcatgga ggcgctggcc atgccccttg cggtggcgct gccgctcttc
     3721 gccctgctcg tgtggttcgc ggtgggccgg gcgttgcggc cgctggcgcg cgtgagcgac
     3781 gagatctcgc gccgcgaccc caaatatctc gcgccgctcg aaggcgaggt gccttcggag
     3841 atcgcccctc tcgtcggacg cctcaacgcg ctcctcgccc gcgtccaatc ctccctcgac
     3901 ggggaacggc gcttcaccag cgatgcggcg cacgaattgc gcacgccgct ggccgccctg
     3961 cgcgcccagc tgcaggtggc ccagggcgcg gccgatgccg ccgggcgcga tcgtgcgatc
     4021 gccaatgccc ttgcggcggg cgagcgagcc acccacctcg tcgagcagct cctcacgctt
     4081 gcgcgcctcg agcacagcgc gtggcaggcg gtcgcggccc ccttcgacct gcaccgcgtt
     4141 gcggcggaag cgatcggcca gcgtgccccg caggcgctcg ccaggcggat cgagctctcc
     4201 ctcgagggcg aggcgggcgc gatggccatg ggccacgccg ggctcgcggc catcgcggtg
     4261 ggaaacctcc tcgacaacgc catccgctac tcgcccgcgg acacggcggt caccgtcgcg
     4321 gtggcaaggg atggcggccg cgccatcttg agcgtgcgcg acgagggtcc gggcatcccg
     4381 gcgggcaggc gtgaagacgc gctgcggcga ttcacgcggc cggaggattc cggcacggag
     4441 ggcagcgggc tgggattgtc catcgtcgcc cgcatcgcgg aactgcacgg ggcggcgctg
     4501 caactgggcg atgcggccgg cggcaagggg ctcgtggcca cgctgcgttt tccggcagcc
     4561 tgagcggggc gggccggcgg gcccgagtgt gagaaaatga cgctccgcag cacgccgccc
     4621 gcccgaagca tctcccgcga agcccccgcc atccatgacg atcgcccagc aagtcgcccg
     4681 ccgccgcacg ttcgcgatca tctcgcaccc cgacgccggc aagacgacgc tcaccgagaa
     4741 gctgctgctc ttcgcgggcg ccatccagat cgccgggagc gtgaaggcgc gcaaggcctc
     4801 gcgccacgcc accagcgact ggatggagat cgagaagcag cgcggcatct cggtggccag
     4861 ttccgtcatg cagatcgagt accgcggctg cgtcgtgaac ctgctcgaca cgccgggcca
     4921 ccaggacttc tcggaggaca cctaccgcgt gctcaccgcc gtggacgcgg cgctcatggt
     4981 gatcgacgcg gccaacggcg tggaagcgca gacgcttcgc ctgctgcagg tgtgccgcgc
     5041 tcgtaacacc cccatcctca ccttcatcaa caagatggac cgcgaggtgc gcaacccggt
     5101 cgacctcgtc gacgagatcg agcgcacgct gggcatggcg gccgtgccct tcacctggcc
     5161 cgtgggcatg ggaaagactt tccacggcgt gtatgacctg cgcaatgcgc gcatgcgcgt
     5221 cttccgcccg ggcgcggacc ggctcaacga cgagggcgac gagatcgtcg ccgacatcga
     5281 cgacgcgggc gtctgcgcgc gcttcggcgc cgaactggtg caggcgaagc aggaaatcga
     5341 gctcgtgcag ggcgcctccc ccgatttcga tcacgccgcc ttcctcgccg gcaggcagac
     5401 gcccgtcttc ttcggctcgg ccatcaacaa cttcggcgtg cgcgaggtgc tcgatgcgct
     5461 ggccgacctg gcgccgccgc ccggaccgcg ggccgccatc cagcgcgagg tggagccccg
     5521 cgaaggcaag ttcaccggcg tagtcttcaa gatccaggcc aacatggatc ccgcgcaccg
     5581 cgaccgcatc gccttcgtgc gcgtgtgctc ggggcagttc gagcgcggca tgcgactcac
     5641 cgtcgcgcgc aacggcaagg tgatacgccc gggcagcgtg gtctcgttcc tgtcgcaacg
     5701 ccgcgagctg ctcgaattcg cctttcccgg cgacatcatc ggcatcccca accatggcac
     5761 gctgcagctg ggcgacacgc tcagcgaagg cgaggtcctg cagttcaccg ggctgccgtt
     5821 cttcgcgccc gaaatattcc aggcggtcga gatcgccgac ccgatgcgca gcaagcagtt
     5881 gaagctgggc ctcgcgcaac tgggcgagga gggcgccatc caggtcttcc acccgcatca
     5941 cggcggggca ctcctgcttg gcgcggtggg ccagctccag ttcgaggtgg tggcccaccg
     6001 cctccagcac gaatacggcg tgccggtgcg gatggtgccg tccaagttcc gcctggcacg
     6061 ctgggtgacg tccgacgacc cgaaggagct cgcgcgcttc atcgaggcca acggccaccg
     6121 cgtggcctat gacgcggtgg atgcgcccac cttcctcgcc acggccaacg ccgagctcgt
     6181 cgtcacccag gatctctgga agggcatcca gttccatgcg cttcgcgagc atgcggggct
     6241 ggtgttccag gcgcggctcg agggctgagc cagtccggcc ttgacacggg tcaagccggg
     6301 gcagcgccag cggttcaatc tagcggcatt tccggatttc ccgatgagct tcgcacaccg
     6361 cctttccctg aaagcccggg tcgtcctgct catgctggcg atcgtcgttc cctttggcgg
     6421 ggtcctcgcc gggtacatcg cgaacgagta cgcggaagcg aggaccaacg cctaccggca
     6481 cgccgaaggg gtcgccgcca tttcggccgc gcgcctgtcc ctgaagctgg gcgacctcga
     6541 ggcgatcctc tcccggctgg cggagcgacc ctcgatccgg gcgctggacc cggagcgctg
     6601 cgacccgatc atcgacgagt atgtccggct gcaccccgag ttcaccacgc tcgcgcttcg
     6661 cgaccgggac ggcaatatcg cctgctcgtt cctcccgggt ccgatagcga aactcgatcc
     6721 gggaaaattc ccctggttcg gggaaggcct gcgcaacgcg aagttcaccg cgggaaatgc
     6781 cttcctcggt caccagaccg ggaaatgggt gtcggtcctg acgcagccgg tccgggacga
     6841 ttcgggcagg acatccggcc tgctggtcat ggccgtcgaa ctgcttcccc tgtccaggca
     6901 gattttcgag ggggtaccgg aaaacctcct gatggcggtt gtcgaccgcg aggggaaggt
     6961 cctgctgcgc tcgcgtgacc acgaggctcg cgtcgggaag gcgattcccg cccatttcgt
     7021 cgagcagacc cgcggcaggg accagggcaa tctcgccgag cgaggcacgg acggcgtgcg
     7081 gcgcctgtac gccttcacga ccattcccgg caccggctgg cgggtgtttg cggcactcga
     7141 cgagaaggaa gccctggcca cctaccgcag ggacctgctc gcgggagttg ccctcggcat
     7201 tcccctcgcc ttgctggtgc tggcgtcggc ctggtgggtc ggttcggcca tcgcgaaccc
     7261 catccgcagt ctcgcccgga cggccgagcg ggtggccgag ggcgacacca atgcgcgcgc
     7321 gccgatcgat ggccccgcgg aaatccggag cgtggcccgg cagttcaacc ggatgctcga
     7381 ctcgttcgag cgccatcgcg aggagcgtgc agccctcgtc ggccatttcg gccagctggc
     7441 gaaggtggcg cccgaaatca tcctgctcat cgaccccgac ggccggatcg tcgaggccaa
     7501 cgacgccgcc gctgcggcct acggctacgg cgtcgacgaa ttgcggggaa tgcacatcct
     7561 cgacctgcgc acgcccgtgg acgcggcgct gctggcgcag cagctgcagg ccgccgacag
     7621 tcccggcggc gtgcttttcg agacgttcca ccggcgcaag gacggcagca ccttccccgt
     7681 ggaagtcagc gcgcggtcgc tcgagatcgg cggcaagctc taccggcaga gcttcgtgcg
     7741 cgacatcacc gcccgccggc aggccgagga tgccatccgc gagagcgagg tgcgattccg
     7801 caccgtcgtc gagtcggccc ccgacggcat cttcatccag accggcgggc gcttcgcgtg
     7861 gctgaaccgg gaagccatgc gcatcttcgg catcgccggc atggaggccg cgcgcgggca
     7921 accggttgcc gagcgtttcc atcccgattc ccgggagctg gcgcgtgcgc gcatccggct
     7981 ggtgaacgag gagcgccgtt ccgttcacct acgagggcga ggacggagcg ctcgtgtacg
     8041 tgcgcgacat caccgaccgc aagcacgccc tggaggaact gcgcaggagc gagtaccgct
     8101 tccgcacgct ggtggagggc gtgccgatca tcgccgtcca ggggtacgac cgcgagcgcc
     8161 gggtggtctt
//
LOCUS       DASUEV010000331        13401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1359311, whole genome shotgun sequence.
ACCESSION   DASUEV010000331 DASUEV010000000
VERSION     DASUEV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 13401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13401
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359311"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2139
                     /locus_tag="VFV55_10465"
     CDS             <1..2139
                     /locus_tag="VFV55_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX4944767.1"
                     /translation="LAVTFTNKAAREMLTRLSAMLPINTRGMWVGTFHGLCNRLLRAH
                     HRDADLPQLFQILDSGDQLSVVKRLAKAQNLDEEKFAPRQLQAFINNCKEAGLRANAV
                     ESGDDFTRRMVAFYADYDAQCNREGVVDFAELLLRTFELLARNLNLLTHYQERFRYIL
                     VDEFQDTNKLQYKWIRMLAGSNGCVFAVADDDQSIYRFRGADVGNVNEFIKDFAVSGV
                     VKLEQNYRSQGNILDAANAVIANNKARLGKNLWTAAGKGEPLRIFAAANDEEEARFTV
                     DEVKELHREGTALADMALLYRSNAQSRILEHSLFRAGISYKVYGGLRFFERQEVKHAL
                     AYLRLAANLDDDTAFSRVVNFPPRGIGARSVEQLQEAAQAGLGSLARAAKAGAVPGRG
                     GTAIAVFMAIVERLRKASEVLTLPELIEEVLAASSLKDHYAAERDGQDRVENLNELVN
                     AATLFNEEFETGVEPAGESQEDVAPIPGTHQQQVLAAFLSHASLEAGEHEAGVGEDAL
                     QLMTVHSAKGLEFDAVFLGGLEEGLFPHDNSLNEDGGIEEERRLMYVAITRARKRLYL
                     TYAGSRMLHGQPRYGIVSRFVQEIPAELCKWIVLPEKQPWASPQKSSWEKPAWGGGGA
                     RGEVWSSGGGRAPKADPAQYATAPRAGFETSRPDEHPFAIGASVRHAKFGEGVVINFE
                     GHGLDARVQVRFRTEGTKWLALQYARLTPA"
     gene            complement(2150..3217)
                     /locus_tag="VFV55_10470"
     CDS             complement(2150..3217)
                     /locus_tag="VFV55_10470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4944768.1"
                     /translation="MAPAAPAQAPPARSRRGFELPRHVYPLRTLGLAAGFVSVAVVFG
                     EVGVHPLAWAALVFHGFAWPHLAYLVARRNADPWSAERRNLVIDSAVGGVWVSLMGFN
                     LLPSVLLVSMMSLDKISVGGPRLLTATWVAMLAGGALTTLAFAPAVRVESSFAVIVAS
                     IPLLVLYPLSVGITTYRLRQRVREQNRRLDHLLRTEQLSGLATRQHWEEAVVREFERC
                     RRTGRTAGLIFIDLDDFKAINDRFGHLVGDEVIRRIGDILRKSLRPSDVACRYGGDEF
                     GVLVPDSGAAGAAAAGARVRAALERLRLPANPELRCTASLGVAEIGPGTASTLEAIGQ
                     ADRALYRAKEAGRNRVHLHEP"
     gene            complement(3223..3957)
                     /locus_tag="VFV55_10475"
     CDS             complement(3223..3957)
                     /locus_tag="VFV55_10475"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX4944769.1"
                     /translation="MDSAIRVRDVRSSRGPAWIAGGWRLFRGATVVWMGLSAGWMLIT
                     FGLVLVPVIGGVAANLMQPVFFSSFALAAHKQLAGTMPQMGDLFAGFRRPLRPLINLG
                     AILLIAEIAIFFLMSLLGLPGVSDGDEVPTIADYVRKLQGKEWILLVGLLLTALVKGA
                     LWFAPAILAFHDLSTAHAVRWSVYAALSNLGAMLAYGIALTVAFFAGVLPWGLGLLVV
                     VPVMLASSYSGYADVFEDAVEAPSAA"
     gene            complement(3971..4921)
                     /locus_tag="VFV55_10480"
     CDS             complement(3971..4921)
                     /locus_tag="VFV55_10480"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX4944770.1"
                     /translation="MSVFTPVSDDEARALLARYTLGELESLEGIAQGIENTNFFLGTT
                     TGKYVLTLFEKIPREDLPFYVGLMHHLAEHDIRCPAPMELQEGGFLTELNGKPAVIVT
                     RLSGVPHLSPTTGQCGKVGTILADIHEAGLEYDAGLANWRGQAWRESFAQRVRPRLSA
                     DEAALIESENRYQSLHDDTVLPQGVIHGDLFRDNVLWDDEGEGGVIDFYFACDDALLY
                     DLAITVNDWCTDSLAHLDPERTRALLDGYDAHRPLTDLERELWPVMLRRAALRTWLGR
                     LEYNHFPKAAEMTIPKDHAFYRRLLEHHIVHARALEAGGD"
     gene            complement(4944..5291)
                     /locus_tag="VFV55_10485"
     CDS             complement(4944..5291)
                     /locus_tag="VFV55_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEX4944771.1"
                     /translation="MRILIATLAFFLAAGAFAQSAPRPLPPGTKPLEEPPPPPAVVET
                     DSALEPQVTIRKEADRTISEYRIKGKLYMMKVTPANGRPYVLIDHKGDGQFARQDNLD
                     SGLRVPQWVLLEF"
     gene            complement(5337..6062)
                     /locus_tag="VFV55_10490"
     CDS             complement(5337..6062)
                     /locus_tag="VFV55_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX4944772.1"
                     /translation="MHKKFPRLSDPQLVNRSRASAREAWRMFEYIAEFVTATERLQAI
                     QPAVSIFGSARVTPGEPSYALAEDISRRLSDAGFAVISGGGPGIMEAANKGCFAGSSP
                     SVGLNIQLPHEQNANQYQDVAHTFQHFFARKVMFVKLSCAFVMLPGGFGTLDELFEVL
                     TLVQTGKVRRVPLILVGSEFWAGLVEWMRGRLAADRMIGPGDVDLLQVLDDPQAVVDA
                     IFTHYHTRGFELSPTEREAEFAL"
     gene            6083..8890
                     /gene="polA"
                     /locus_tag="VFV55_10495"
     CDS             6083..8890
                     /gene="polA"
                     /locus_tag="VFV55_10495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX4944773.1"
                     /translation="MNDSKKRLLLVDASSYLYRAFHAMPEFRSPAGEPTGAIMGVVNM
                     LRKLASDYPSDYIAAVFDPKGKTFRDDIYPDYKATRQAMPEDLAAQIAPLFETIRALG
                     WPLVVVDGVEADDVIGTLARHAESVHGWDTLISTGDKDLTQLVSERVTWMNTMSNETL
                     DPKAVEAKFGVPPDRIVDYLALMGDAVDNVPGVDKVGPKTAAKLVQQYGSLDAVMAHA
                     DEVKGAVGENLRKALEWLPTGRTLVTVKTDVPLPFEVESLVQGAPDKAKLAALYERFG
                     FRSLREALDKGENGGTGAAPVRSPAPAGRASDSAASGGSAGSGKTDARPEPARAVFVD
                     ARRYETVLDEAALDRWLAKITAADLTCFDTETTSLDPMAAQLVGLSFCVEAGEAAYVP
                     LAHRYPGAPDQLDFGRTLARLRPWFEDERRRKVGQNAKYDCHVLANHGIAVRGVVHDT
                     LLASYVLESNQRHDMDSLAMRHLGAKTILYEEVAGKGASSICFDQVSVERATEYSAED
                     ADVTFQLHGALWPRVEADAKLRHIYTDIEMPVSGVLLVMERNGVLIDSALLATQGGEL
                     GARMMALEATVHELAGQPFNLGSPKQLGEILFERMKLPVVKKTPSGAPSTDEEVLEKL
                     ALDHPLPKALLEHRAMSKLKSTYTDKLPRMVNPRTGRVHTNYAQAVAVTGRLSSTDPN
                     LQNIPVRTAEGRRIREAFIAPPGSKIVSADYSQIELRIMAHLSGDKGLIEAFAAGEDI
                     HRHTASEVFGVSPGDVTSQQRRYAKVINFGLIYGMSAFGLAGNLGIERAAAQSYMDKY
                     FSRYPGVAAYMERTRNEAKERGFVETVFGRRLWLPDIASANQARRQGAERQAINAPMQ
                     GTAADLIKLAMIAVQGWIDSERLATKLIMQVHDELVLEVPEGELARVKPQVEKLMTGV
                     AALDVPLVVEAGVGENWERAH"
     gene            9122..9349
                     /locus_tag="VFV55_10500"
     CDS             9122..9349
                     /locus_tag="VFV55_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944774.1"
                     /translation="MTLQLDSLIAIAFIVPVVFFVILNIVLYRTRGYVSAVKPVAEVA
                     PKVETTGPVGAEAANPVGAEAANDSTIREAA"
     gene            9559..10566
                     /locus_tag="VFV55_10505"
     CDS             9559..10566
                     /locus_tag="VFV55_10505"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX4944775.1"
                     /translation="MSNPSCPTAPPEAVSLAGAVAELVRGNLVAFPTETVYGLGADAT
                     NPDAVAKIFAAKGRPADHPLIVHLPPEASIDDWAIEVPGAARSLARAFWPGPLTLILK
                     KSARVPAAVTGGQDTVGLRIPSHPVAQRLLREFAKVGSGAVAAPSANRFGRVSPTSAQ
                     HVRDEFGPLLVVVEGGDCEVGLESTIVDLSRGRATVLRPGGITREEIAAVLGAAPGER
                     DAASPRAPGTLAAHYAPGKPLALVEGESLDMEIARGPAVAVLAFRKRPPGAAAIATWI
                     DARGDPAGYGHDLYANLRRLDASDAVRILVERPPGSVAWEAVSDRLSRAAAGTGGPET
                     P"
     gene            10657..13011
                     /locus_tag="VFV55_10510"
     CDS             10657..13011
                     /locus_tag="VFV55_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX4944776.1"
                     /translation="MTWRAGLFLKYFAFIGLLVSVGLVASGAINLYFSYEETRASFVN
                     LQREKAVTAAVRIEQFVRDIESQLGWTTLPMVAPGQDAMEMRRLDFLKVLRQLPAVTE
                     VSHIDERGWEQLKVSRLAMDTVGSNTDFSADPRFTEPRKGRTWFGPVYFRKETEPYMA
                     VAIPEARDRKGVTVVEVNLKFIWDVVSRIRVGKDGYAYVVDGKGFLVAHPDISLVLRK
                     ADLSSLPQVKAALEAKPDAQWKELPDARDSRGKPVLAAYATIEALGWRVLVEEPTSAV
                     LAPLYDSLLRTAALLLAGVVLSILASLFLARRMVNPVRALQRGAERIGAGDLAGRIDV
                     RTGDELETLASRFNAMAGQLGESYASLERKVEERTAELRETLEQQTATAEVLKVISGS
                     PTDEMPVFEAIVRAVAGLGGATTAAAFRYGGGKIHYVTSFGHSEEWRAHVAKASPWSA
                     DSTTIAGRVILSESPCQIEDSLADPDYDKTAARLGGGRRMLGVPMLRDGLPIGAFTVT
                     WPTPGPVPEKFIRVLQTFADQAVIAIENVRLFNETQDKSRQLEIANKHKSQFLANMSH
                     ELRTPLNAIIGFSEALDERMFGELNGKQAEYVADIHTSGKHLLLLINDILDLSKVEAG
                     RMELDLAEFDLPTALQDAMTLVRERAQHHGITLTFEAEAALGRFTADERKLKQIMLNL
                     LSNAVKFTPEGGRIGVSARRADGAVEIAVSDTGVGIAPEDQRAVFEEFRQVGRDYTRK
                     AEGTGLGLALARRFVELHGGTLGVASEPGRGSTFTFTLPFRHGQ"
     gene            13001..13390
                     /locus_tag="VFV55_10515"
     CDS             13001..13390
                     /locus_tag="VFV55_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX4944777.1"
                     /translation="MANEHVLLIEDDEKNRKLARDILLFRGYRVSEAESAEAGLAIAA
                     ADPPALVIMDIELPGIDGIEALARLRAGEATRGIPVIAFTASVMPGERSTIMDAGFDA
                     FLPKPIAIREFTDTVARQIARPTGRAP"
BASE COUNT         2253 a         4453 c         4737 g         1958 t
ORIGIN      
        1 ctcgccgtca ccttcaccaa caaggcggcg cgcgagatgc tgacgcggct gtcggcgatg
       61 ctgccgatca acacgcgcgg catgtgggtg ggcactttcc acggcctgtg caaccggctc
      121 ctgcgcgcgc accaccgcga cgcggatctt ccgcagctct tccagatcct cgattccggc
      181 gaccagctct cggtggtgaa gcgcctggcc aaggcgcaga acctggacga ggagaagttc
      241 gcgccgcgcc agttgcaggc cttcatcaac aactgcaagg aagcggggct gcgcgccaac
      301 gcggtcgagt cgggcgacga cttcacgcgc cgcatggtcg ccttctacgc cgactacgac
      361 gcgcagtgca accgcgaggg cgtggtggat ttcgcggaac tgctgctgcg caccttcgag
      421 ctgctcgcgc gcaacctcaa cctgctgacc cactaccagg agcgcttccg ctacatcctg
      481 gtggacgagt tccaggacac caacaagctg cagtacaagt ggatccgaat gctggcgggg
      541 tccaatgggt gtgtcttcgc agttgccgac gacgatcagt ccatttaccg gttcaggggg
      601 gcggacgtcg gcaatgtcaa tgagttcatc aaggactttg ccgtttccgg ggtggtaaag
      661 ctcgagcaga actaccgctc gcagggaaat attctcgatg ctgcgaacgc agtgatcgcg
      721 aacaacaagg cccgcctggg caagaacctg tggaccgccg cgggcaaggg cgagccgctg
      781 cgcatcttcg ccgccgcgaa cgacgaggag gaggcccgct tcacggtgga cgaggtgaag
      841 gagctgcatc gcgagggcac ggcgctcgcc gacatggcgc ttctctaccg ctccaacgcg
      901 cagtcgcgca tcctcgagca ctcgctcttc cgtgccggca tctcgtacaa ggtgtacggc
      961 ggcctgcgct tcttcgagcg ccaggaagtg aagcacgcgc tcgcctacct gcgcctggcc
     1021 gccaatctcg acgacgacac cgccttctcg cgcgtggtga attttccgcc gcgcggaatc
     1081 ggggcaagga gcgtcgagca gctccaggaa gcggcgcagg cggggctggg cagtcttgca
     1141 cgcgccgcga aagcgggcgc ggtgccgggg cgcggcggca cggccatcgc ggtcttcatg
     1201 gcgatcgtgg agcggttgcg caaggcctcc gaggtgctca cccttcccga gctcatcgag
     1261 gaggttctcg ccgcctcgtc cctcaaggac cattacgcgg ccgagcgcga cgggcaggac
     1321 cgcgtcgaga acctcaacga actggtgaac gcggccacgc tcttcaacga ggagttcgag
     1381 accggcgtcg agcccgcggg agaatcccag gaggacgtgg cccccatccc cgggacgcac
     1441 cagcagcagg tgctggcggc gttcctttcg cacgcgagcc tcgaggccgg cgagcacgag
     1501 gccggcgttg gcgaggacgc gctgcaactg atgaccgtgc actccgccaa gggactcgag
     1561 ttcgacgcgg tgttcctcgg aggcctcgag gaaggcctct tcccgcacga caacagcctg
     1621 aacgaggacg gcggcatcga ggaggagcgg cgcctcatgt acgtggccat cacccgcgcg
     1681 cgcaagcgcc tgtacctcac ctacgcgggc agccgcatgt tgcacggcca gccgcgctac
     1741 ggcatcgtct cgcgtttcgt gcaggagatc ccggcggaac tgtgcaagtg gatcgtgctg
     1801 ccggaaaagc agccctgggc ttcgccgcag aagtccagct gggagaagcc cgcgtggggc
     1861 ggcggaggag cgcgcgggga ggtctggtct tccggcggcg gccgcgcgcc gaaggcggac
     1921 cctgcccaat atgcgaccgc gccgcgtgcc ggcttcgaga cctcgcgccc cgacgagcat
     1981 cccttcgcca tcggcgcgag cgtgcgccac gcgaagttcg gcgagggcgt ggtgatcaat
     2041 ttcgagggcc atggcctcga tgcaagagtg caggtgcgct tccgcacgga aggcaccaag
     2101 tggctggcgc ttcagtacgc caggctcacg ccggcatgag catcgcgcgc tagggctcgt
     2161 gcaggtggac gcggttgcgt ccggcttcct tggcgcggta aagggcgcgg tcggcctggc
     2221 cgatcgcttc gagcgtgctt gcggtgcctg gcccaatttc cgccacgccc agactcgcgg
     2281 tgcagcgcag ttccgggttc gcgggcaacc gcagccgttc gagcgccgcg cggacgcggg
     2341 cgccggccgc cgccgcccct gccgctcccg agtccggaac gaggacgccg aattcgtcgc
     2401 cgccgtagcg gcacgccacg tccgagggcc gcagcgactt gcgcaggatg tcgccgatgc
     2461 ggcggatcac ctcgtcgccg acgaggtgac cgaaccggtc gttgatcgcc ttgaaatcgt
     2521 ccaggtcgat gaagatcagc cccgcggtcc ggccggtgcg ccggcaccgc tcgaactcgc
     2581 gcacgacagc ctcttcccag tgctggcgcg tggcgaggcc cgaaagctgc tccgtgcgca
     2641 gcaagtggtc gaggcggcgg ttctgctcgc gcacgcgctg gcgcaggcgg taggtggtga
     2701 tgccgaccga gagcggatag agcacgagca ggggaatgct ggcgacgatg accgcaaagc
     2761 tcgactcgac gcgcaccgcg ggcgcgaacg cgagggtcgt caaggcaccg cccgcgagca
     2821 tcgcgaccca ggtcgcggtg agcaatcgcg gccccccgac gctgatcttg tcgaggctca
     2881 tcatggacac cagcagcacg ctcggcagga ggttgaagcc catgagcgac acccacacgc
     2941 cgccgaccgc cgaatcgatg acgaggttgc ggcgctcggc gctccacgga tcggcattcc
     3001 tgcgcgcgac aaggtaggcc aggtgcggcc aggcgaaccc gtggaagacg agtgccgccc
     3061 aggcgagcgg gtgcacaccg acctcgccga acacgactgc cacactcacg aagcccgcgg
     3121 cgaggccgag ggtgcgaaga ggatagacat gccggggcag ttcgaagccg cggcgggagc
     3181 gggcaggagg agcctgcgct ggggcggccg gcgccatccg gttcaggcgg ccgacggcgc
     3241 ctcgactgcg tcctcgaaga cgtccgcgta gcccgaatag gagctggcca gcatcaccgg
     3301 caccaccacc agcagtccca ggccccaggg caggacgccg gcgaagaagg cgacggtgag
     3361 cgcgatcccg taggcgagca tggcacccag gttggacagc gcggcgtaaa cgctccagcg
     3421 caccgcgtgg gcggtggaaa ggtcgtggaa ggccaggatg gccggcgcga accaaagggc
     3481 gcccttcacc agcgcggtga gcagcaggcc gacgagcagg atccattcct tgccctgcag
     3541 cttccggacg taatcggcga tcgtgggaac ctcgtccccg tcactcacgc ccggaagccc
     3601 gagcagggac atcaggaaga agatcgcgat ctccgcgatg agcaggatgg ccccgagatt
     3661 gatgagcggc cgcagcggcc ggcgaaaccc ggcgaagaga tcgcccatct gcggcatcgt
     3721 gccggcaagc tgcttgtgcg cggccagggc gaagctcgag aagaacacgg gctgcatcag
     3781 gttcgcggcc acgccgccga tcaccggcac cagcaccagc ccgaaggtga tcagcatcca
     3841 gcccgcggac aggcccatcc acaccacggt tgcgccgcgg aacaggcgcc agccccccgc
     3901 gatccaggcg ggcccgcgcg aggagcgcac gtcgcgaacg cgaatggcgg aatccaaggg
     3961 gcgtgggtcc tcagtccccg ccggcttcca gcgcgcgcgc gtgaacgatg tggtgctcga
     4021 ggaggcgccg gtagaaggcg tggtccttcg gtatcgtcat ttcggcggcc ttggggaagt
     4081 ggttgtactc caggcgcccg agccaggtgc gaagcgccgc gcgccgcagc atcaccggcc
     4141 acagttcgcg ctcgaggtcg gtgaggggcc gatgggcgtc gtagccatcg aggagggcgc
     4201 gcgtgcgttc cggatcgagg tgcgcgaggg aatccgtgca ccagtcgttc accgtgatgg
     4261 cgaggtcgta gagcagggca tcgtcgcacg cgaagtagaa gtcgatgacc ccgccctcgc
     4321 cctcgtcgtc ccagagcacg ttgtcgcgga acaggtcgcc gtggatcacg ccttgcggca
     4381 ggaccgtgtc gtcgtgcagc gactggtagc ggttctcgct ctcgatgagg gcggcttcgt
     4441 ccgcggagag cctcggccgc acgcgctggg cgaagctctc gcgccaggcc tggccgcgcc
     4501 agttggcgag cccggcgtcg tactcgaggc ccgcctcgtg gatgtcggcg aggatggtgc
     4561 ccaccttgcc gcactgcccg gtcgtgggcg acaggtgcgg cacgccggac aggcgcgtca
     4621 cgatgacggc gggcttgccg ttgagttccg tgaggaagcc gccctcctgc agttccatcg
     4681 gcgccgggca gcggatgtcg tgctcggcga ggtggtgcat caggcccacg tagaagggca
     4741 ggtcctcgcg cgggatcttc tcgaagagcg tgagaacgta cttcccggtg gtcgtgccga
     4801 ggaagaagtt ggtgttctcg attccctggg cgatgccctc gagggattcg agctcgccga
     4861 gggtgtagcg cgcgaggagg gcgcgcgcct cgtcgtcgga gactggcgtg aagactgaca
     4921 tcgcggggag ccgttgccgg aagctagaat tcgaggagca cccactgggg aacgcgcagg
     4981 cccgaatcca ggttgtcctg gcgggcgaac tgcccgtcgc ccttgtggtc gatcagcacg
     5041 tagggcctgc cgttggcggg ggtgactttc atcatgtaga gcttgccctt gatacggtat
     5101 tcggagatcg tgcggtccgc ttccttgcgg atggtgacct ggggctcgag ggccgaatcg
     5161 gtctccacga cggcaggcgg aggcggcggc tcctcgaggg gcttggttcc cgggggaagc
     5221 gggcgcggcg cggactgcgc gaaggcaccg gcggcgagga agaaggcgag ggtggcaatg
     5281 aggatgcgca tggcgtcacg cggaattgag ggccagttta gcacttggac aggcgctcac
     5341 aacgcgaatt ccgcctcgcg ctcggtgggc gaaagctcga agccgcgcgt gtggtagtgg
     5401 gtgaagatcg cgtccaccac cgcctgcggg tcgtccagca cctgcagcag gtccacgtcg
     5461 ccggggccga tcatgcggtc ggcggcgagg cggccgcgca tccattccac gagaccggcc
     5521 caaaactcgc tgcccacgag gatgagggga accctgcgca cctttcccgt ctgcaccagg
     5581 gtgagcacct cgaacagctc gtcgagcgtg ccgaagccgc ccggcagcat cacgaaggcg
     5641 caggagagct tcacgaacat caccttgcgc gcgaagaagt gctggaaggt gtgcgccacg
     5701 tcctggtact ggttggcgtt ctgctcgtgc ggaagctgga tgttcagccc gacgctgggg
     5761 ctggacccgg cgaagcaacc cttgttggcc gcctccatga tgccggggcc gccgcccgag
     5821 atgaccgcga agcccgcgtc ggaaagccgc cgcgagatgt cctcggccag cgcgtaggat
     5881 ggctcgcccg gcgtcacgcg cgcgctgccg aatatcgaca ccgccggctg gatcgcctgc
     5941 aggcgctctg tggcggtgac gaactctgcg atatattcga acatccgcca cgcctcgcgc
     6001 gccgaggccc gcgagcgatt caccagttgc ggatcggata gtctcggaaa cttcttgtgc
     6061 atggcggccc ctgagcggtg aaatgaacga ttcgaagaag cgcctgctgc tcgtcgacgc
     6121 ctcgagctac ctgtatcgcg ccttccacgc gatgccggaa ttccgcagcc ccgcgggaga
     6181 acccacgggc gcgatcatgg gcgtggtgaa catgctgcgc aagctcgcgt ccgactatcc
     6241 gtccgattat atcgccgcgg tcttcgaccc caagggcaag accttccggg acgacatcta
     6301 tcccgactac aaggccacgc gccaggcgat gcccgaggac ctcgcggcgc agatcgcgcc
     6361 gctcttcgag acgatccgcg cgctgggctg gccgctcgtc gtcgtcgacg gcgtcgaggc
     6421 cgatgacgtg atcggaacgc tcgcgcgcca cgccgagtcg gttcacggct gggacacgct
     6481 catctccacg ggcgacaagg acctcacgca actggtgagc gagcgcgtca cgtggatgaa
     6541 cacgatgtcc aacgagacgc tcgacccgaa ggccgtcgag gccaagttcg gcgtgccgcc
     6601 cgatcgcatc gtcgattacc tcgcactcat gggcgacgcc gtggacaacg ttccgggcgt
     6661 ggacaaggtg ggccccaaga ccgccgcgaa gctcgtccag cagtacggct cgctggacgc
     6721 agtgatggcg catgcggacg aagtgaaggg cgcggtgggc gagaacctgc gcaaggccct
     6781 cgagtggctg cccaccgggc gcaccctggt cacggtgaag acggacgtcc ccctgccctt
     6841 cgaggtcgag tcgctggtgc agggcgcgcc ggacaaggcg aagctcgccg cgctctacga
     6901 gcgtttcggc ttccgcagcc tgcgcgaggc gctcgacaag ggcgagaacg gtggaacggg
     6961 agcggcaccg gttcgcagcc cggcgcctgc aggccgcgcg tccgattccg ccgcttccgg
     7021 ggggtccgcg ggttcgggaa agacggatgc ccggcccgag cctgcccgcg ccgtcttcgt
     7081 cgatgcgcgc cgctacgaga ccgttctcga cgaggccgcc ctcgatcggt ggctggcgaa
     7141 gatcacggcg gcggatctca cctgcttcga taccgagacc accagcctcg accccatggc
     7201 ggcgcagctc gtgggcctgt ccttctgcgt ggaggcggga gaggcggcct acgtgccgct
     7261 cgcgcaccgc taccccggcg cgccggacca gctcgacttc gggcgaacgc tggcgagact
     7321 caggccctgg ttcgaagacg agcgccggcg caaggtcggc cagaacgcca agtacgactg
     7381 ccacgtgctc gccaatcacg gcatcgcggt ccgcggagtc gtgcacgaca cgctgctcgc
     7441 ctcctacgtg ctggaaagca accagcgcca cgacatggat tcgctcgcca tgcgccacct
     7501 tggcgcgaag acgatcctct acgaggaggt cgcgggcaag ggtgcgtcgt ccatctgctt
     7561 cgaccaggtg agcgtggagc gcgcgacgga atactcggcc gaggatgccg acgtcacctt
     7621 ccagctgcac ggcgcgctct ggccgcgcgt ggaggcggat gcgaagctgc gccacatcta
     7681 caccgacatc gagatgccgg tgtcgggggt gctgctggtg atggagcgca acggcgtgct
     7741 catcgattcg gcgctgcttg ccacgcaggg cggcgagctg ggcgccagga tgatggccct
     7801 cgaagcgacg gtgcacgagc tcgccgggca acccttcaac ctcggcagcc cgaagcaact
     7861 gggcgagatc ctcttcgagc gcatgaagct gcccgtggtg aagaagacgc cctcgggcgc
     7921 gccgtccacg gacgaggagg tgctggagaa gctggcgctg gaccacccgt tgcccaaggc
     7981 gctcctcgag caccgcgcga tgtcgaagct gaagagcacc tataccgaca agctgccgcg
     8041 catggtgaat ccgcgcaccg gtcgcgtgca caccaactac gcccaggccg tggcggtgac
     8101 ggggcgcctg tcgtcgacgg accccaacct gcagaacatc ccggtgcgca cggcggaggg
     8161 ccggcgcatc cgcgaggcct tcatcgcgcc gcccggctcg aagatcgtgt cggcggacta
     8221 ctcgcagatc gagctgcgca tcatggccca cctgtcgggg gacaagggcc tcatcgaggc
     8281 cttcgcggcc ggcgaggaca tccaccgcca taccgcctcg gaagtgttcg gcgtatcgcc
     8341 cggcgacgtc acgagccagc agcgccgcta cgccaaggtg atcaacttcg gcctcatcta
     8401 cggcatgagc gccttcggcc tcgcgggcaa cctcggcatc gagcgggcgg ccgcgcagag
     8461 ctacatggac aagtacttct cgcgctaccc cggcgtggcg gcgtacatgg aacgcacgcg
     8521 caacgaggcg aaggagcgcg gcttcgtgga gaccgtgttc ggccgccggc tgtggcttcc
     8581 cgacatcgcc tcggccaacc aggcgcgccg ccagggtgcc gagcggcagg cgatcaacgc
     8641 gccgatgcag ggaacggcgg ccgacctgat caagctcgcc atgatcgcgg tgcagggctg
     8701 gatcgattcc gaacggctcg cgacgaagct catcatgcag gtgcacgacg agctggtgct
     8761 cgaggttccg gagggcgagc tcgcgcgcgt gaagccgcag gtcgagaagc tgatgaccgg
     8821 ggtggccgcc ctcgacgttc cgctggtcgt ggaggcgggg gtgggggaga actgggaaag
     8881 ggcgcattag cctcggtgtc agactccgag ggtaatccgg caccgccaat gtggccgtga
     8941 tcgagccgtg gctgcgcaag catgcgcgac gaattcggcc gggcgcgtga atagttggca
     9001 cgccttccgc ggaattgttc ctcggtcccg atacaggtgg cgcgtagcct tggtctcgag
     9061 cgcgcaccgt gcgcgccgcc ccggtgaccg gggcaaccca gccagggaca aggagccgaa
     9121 catgacactc caactcgaca gcctcatcgc catcgcattc atcgtgccgg ttgtgttctt
     9181 cgtgattctc aacatcgttc tttaccgcac tcgcggatac gtttcggccg tgaagccggt
     9241 tgcggaggtt gcgccgaagg tggagacgac gggtccggtt ggcgccgaag ccgcgaatcc
     9301 ggttggcgcc gaagccgcga acgacagcac gattcgcgaa gcggcctgac ggcgacgatc
     9361 caggccatga aagcgcgcgg atcccgatcc aggtttcgcc ggcaccatca ccggtgccgc
     9421 aagggccgga gccccggacc aggcggtacg gcgcgcgggg ccgggaggat ggtcctcggc
     9481 cacgtgccgc aatcctgggc ggtggacgcg gcaaacgacc cgcaggcgtg agcgcgccgc
     9541 cgggcgataa tgcggggcat gagcaacccg tcctgcccga ccgccccacc cgaagcggtt
     9601 tcccttgccg gtgcggtcgc ggaactcgtc cgcggcaatc tcgtggcgtt cccgacggag
     9661 acggtctacg gcctcggggc ggacgcgacc aatccggatg ctgtcgcgaa gatcttcgcg
     9721 gcgaaggggc ggccggcaga tcatccgctc atcgtgcacc tgccgccgga ggcgagtatc
     9781 gacgactggg ccatcgaggt tcccggcgcg gcgcgcagcc tcgcgcgcgc cttctggccc
     9841 ggcccgctga cactgatcct gaagaagtcg gcgcgcgtcc cggccgcggt caccggcggg
     9901 caggatacgg tgggacttcg cattccctcc catccggtgg cgcagcggct cctgcgcgaa
     9961 ttcgcgaagg tcggcagcgg cgcggtggcg gcgccgtcgg cgaaccgttt cggccgcgtg
    10021 agccccacct ccgcgcagca cgttcgcgac gaattcggtc cgctccttgt cgttgtcgaa
    10081 gggggtgact gcgaggtcgg gctcgagtcg acgatcgtgg atctctcgcg gggccgcgca
    10141 acggtgctgc gaccgggcgg catcacgcgg gaggagatcg ccgccgtgct gggcgccgcg
    10201 ccgggggaac gggatgccgc ctctccccgt gcaccgggca cgctggccgc gcattacgcg
    10261 ccaggcaagc ccctcgcgct ggtcgaaggc gaatcgctcg acatggaaat cgcccgcggg
    10321 ccagcggttg cggtcctcgc cttccggaag cggccgcccg gcgccgcggc aatcgcgaca
    10381 tggatcgacg cgcgcggcga ccccgccggc tacggccacg acctctatgc caacctgcgg
    10441 cggctcgacg cgagtgacgc cgtgcgcatc ctcgtcgagc gcccgccggg cagcgtggcc
    10501 tgggaggccg tgagcgatcg cctgtcgcgc gcggcggcgg gaacaggcgg gcccgaaacg
    10561 ccctgagcgt cggcggcacc aacgcgccgg caggcgcgcg tgaatattca cctgcggcgc
    10621 cgggcggcct gcggtagcct tgttcggggt gatcgaatga cctggcgcgc cggcctcttc
    10681 ctcaagtact tcgcgttcat cggcctcctg gtgagcgtgg ggctcgttgc gagcggtgcc
    10741 atcaacctct acttctcgta cgaggagacg cgcgcctcct tcgtgaacct gcagcgcgag
    10801 aaggccgtca cggcggcggt gcgcatcgag cagttcgtgc gcgacatcga aagccagctc
    10861 ggctggacga cgctgccgat ggtggcgccg gggcaggacg cgatggagat gcgccgcctc
    10921 gatttcctga aggtcctgcg ccagttgccc gccgtcacgg aggtgagcca catcgacgag
    10981 cgcggatggg agcaactcaa ggtttcgcgg ctcgcgatgg acacggtcgg cagcaacacg
    11041 gatttctccg ccgacccgcg cttcacggag ccgcggaagg gacgcacgtg gttcggcccg
    11101 gtgtacttcc gcaaggagac cgagccctac atggccgtcg cgattcccga ggcgcgcgac
    11161 cgcaagggcg tcacggtggt ggaggtgaac ctcaagttca tctgggacgt ggtctcgcgc
    11221 atccgcgtgg ggaaggacgg ctacgcctac gtggtcgacg gcaagggctt cctcgtggcc
    11281 cacccggaca tcagcctcgt gctgcgcaag gcggacctgt cctcgcttcc ccaggtgaag
    11341 gcggcgctcg aggcgaagcc cgacgcgcag tggaaggagc ttcccgacgc gcgcgacagc
    11401 aggggcaagc ccgtgctcgc cgcgtacgcg acgatcgagg cgctgggatg gcgcgtcctc
    11461 gtcgaggagc cgacgagcgc ggtcctggcc ccgctctacg attcgctcct gcgcacggcg
    11521 gccctcctgc tcgcgggcgt ggtgctgtcg atcttggcaa gccttttcct cgcgcggcgc
    11581 atggtgaacc cggtgcgggc gctgcagagg ggcgcggagc gcatcggcgc gggcgacctt
    11641 gcgggccgga tcgacgtgcg cacgggagac gagctggaga cgctcgcgag ccgattcaat
    11701 gcgatggcgg ggcagctcgg ggaatcgtac gcgagcctcg aacgaaaggt cgaggagcgc
    11761 accgcggaac tgcgcgagac cctggagcag cagacggcga ccgcggaagt gctcaaggtc
    11821 atcagcggct cgccgacgga cgagatgccg gtcttcgagg cgatcgtgcg cgccgtcgcg
    11881 ggactcggcg gcgcgacgac ggcggcggca ttccggtatg gcggagggaa gattcactac
    11941 gtgacgagct tcggccattc ggaggaatgg cgggcccacg tcgcgaaggc ttcgccgtgg
    12001 agcgcggatt caacgaccat cgcggggcgc gtcatcctga gcgagtcgcc ctgtcagatc
    12061 gaggacagtc tagctgaccc ggattacgac aagaccgcgg cgcggttggg cggcgggcgc
    12121 cggatgctcg gtgtcccgat gctccgcgac ggcctgccga tcggcgcgtt caccgtaacc
    12181 tggccgacgc ccggcccggt gccggaaaag ttcatccgcg tgctccagac gttcgccgac
    12241 caggcggtga tcgcgatcga gaacgtgcgc ctgttcaacg agacccagga caagagccgc
    12301 cagctcgaga tcgccaacaa gcacaagagc cagttcctcg ccaacatgtc gcacgagctg
    12361 cgcacgccgc tcaacgccat catcggtttc tcggaagcgc tcgatgagcg catgttcggt
    12421 gaactgaacg gcaagcaggc ggagtacgtc gcggacatcc acacctccgg caagcacctg
    12481 ctcctgctca tcaacgacat cctcgacctt tccaaggtgg aggccgggcg catggagctc
    12541 gacctcgccg agttcgacct gcccacggcc ctccaggacg ccatgacgct cgtgcgcgag
    12601 cgggcgcagc accacggcat cacgctgacc ttcgaggccg aggcagcgct gggccggttc
    12661 accgccgacg agcgcaagct caagcagatc atgctcaacc tgctatccaa cgcggtgaag
    12721 ttcacgcccg agggcggccg catcggcgtg tcggcccgtc gcgcggacgg cgccgtcgag
    12781 atcgcggttt cggacacggg cgtgggcatt gctccggagg accagcgggc ggtgttcgag
    12841 gagttccgcc aggtcgggcg ggactacacg cgcaaggccg agggcacggg gctggggctc
    12901 gcgctcgcca ggcgtttcgt cgagctgcac ggcgggacac tgggcgtggc gagcgagccc
    12961 ggcaggggat cgaccttcac tttcacgcta cccttccgcc atggccaatg aacacgtgct
    13021 gctgatcgag gacgacgaga agaaccgcaa gctcgcgcgc gacatcctgc tgttccgggg
    13081 ctatcgcgtc tcggaagccg aatcggcgga agccggcctg gcaatcgccg ctgccgatcc
    13141 gcccgccctc gtcatcatgg acatcgagct gccgggcatc gacggcatcg aggcgctcgc
    13201 gcggctgcgc gccggcgagg ccacgcgagg catcccggtg atcgcgttca cggcctcggt
    13261 gatgcccggg gagcgcagca ccatcatgga cgcgggcttc gacgcgttcc tccccaagcc
    13321 gatcgcgatc agggaattca ccgacacggt cgcccggcag atcgccaggc cgaccgggag
    13381 ggccccatga cggagaaggc g
//
LOCUS       DASUEV010000332         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_105372, whole genome shotgun sequence.
ACCESSION   DASUEV010000332 DASUEV010000000
VERSION     DASUEV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105372"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFV55_10520"
     CDS             complement(<1..370)
                     /locus_tag="VFV55_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX4944778.1"
                     /translation="MRVRGVVLVALVAVLVAVLAAACGPSAPRLPKLEAGAVILAFGD
                     SLTYGAGAAPEQAYPAALERIISRKVVAAGVPGEISQEGLRRLPGVLDEVRPQLLILC
                     HGGNDFLRRLDEGAAAGNVRE"
     gene            complement(367..909)
                     /locus_tag="VFV55_10525"
     CDS             complement(367..909)
                     /locus_tag="VFV55_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX4944779.1"
                     /translation="MALKSTIFKVELTVSDLDRGHFETHALTIARHPSETDERMMVRL
                     LAFALNADERLAFGRGLSSEGEADLCLADRTGAIDLWIDVGLPDERDVRKASGRADRV
                     AVYTYGTGSERWWKQNAAAMERIANVAVVEFPIETTRAMAARAARNMKLQCTIQDGHV
                     WLADDDVSLAIEPLVRKAAA"
     gene            complement(921..1196)
                     /locus_tag="VFV55_10530"
     CDS             complement(921..1196)
                     /locus_tag="VFV55_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944780.1"
                     /translation="MKLTELHKNQGLKIEGEARRGGVSDRYGRGVAHVTDRKEQRKLD
                     AAAGLVPFACKLHGDLIKELQARAEASGASLNETVAEMLKKGLAAKK"
     gene            1289..2758
                     /locus_tag="VFV55_10535"
     CDS             1289..2758
                     /locus_tag="VFV55_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX4944781.1"
                     /translation="MTARPDIPAFAAGPLAARIERQWDSDIIPQLVDYIRIPARSPHF
                     DADWAKNGHIEAAVRQAEKWVTAQAVKGLSLEIVRLGERTPVLFFDIPATGGLAGERT
                     VLFYGHLDKQPEMTGWRDGLGPWTPVIEDGKLYGRGGADDGYAVFAALSAVMALDAEG
                     VARPRCVGIIETCEESGSYDLPAYLHALAPRMGDVQLVVALDSGAGNFEQLWVTTSLR
                     GLVNGTLDVRILEEGVHSGDAGGVVPSSFRIARRILDRIDDSSTGAVLPVAFHCDIPA
                     DRLEQARQAARILGDATWKRFPWVSCCDDAGANRVFAEPVTKDPVELILNRTWRAALA
                     VTGADGLPPTNFAGNVQRPFTSLKLSLRLPPSVDAASASEIFKQALEADPPHGARVSF
                     TCDSAATGWNAPSLTPWLSAALDAASQASFGKPAAYMGEGGTIPFMGMLGVKFPNAQI
                     LVTGVLGPKSNAHGPNEFLHLSYAKKVTVATAMVIAAVR"
     gene            2758..>3221
                     /locus_tag="VFV55_10540"
     CDS             2758..>3221
                     /locus_tag="VFV55_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX4944782.1"
                     /translation="MRRRTVAAGLILVLAAALGGWWLSRQDKAPQAAAKGGGVAVPVT
                     VATVERRDVPVRLLSNGTVVTLQSVDIRAQITSTVREVHIREGQNVAKGDLLVSLDSR
                     AEEANLKKAEAQVEKDRADLANGLRTLERQRQLFAQKFISQSALDAAQNQVET"
BASE COUNT          489 a         1122 c         1113 g          497 t
ORIGIN      
        1 tctcgcgcac gttcccggcg gccgcgcctt cgtcgagcct cctcaggaag tcgttgccgc
       61 catggcacag gatgaggagt tgcggccgca cttcatcgag caccccgggc agccgccgga
      121 gaccctcctg cgaaatctcg ccgggaacgc cggcggcgac caccttgcgc gagatgatcc
      181 gctcgagcgc cgccgggtag gcctgttccg gcgcggcacc ggcgccgtag gtgaggctgt
      241 cgccgaaggc gaggatgacg gcaccggctt cgagcttcgg cagcctgggc gccgacgggc
      301 cgcacgcggc cgcgagcacc gccacgagca ccgccacgag cgcaacgagg acaactccgc
      361 gcaccctcac gcggccgcct tgcgcaccag cggctcgatc gcgagcgaca cgtcgtcgtc
      421 ggcaagccac acatggccgt cctggatggt gcactgcagc ttcatgttgc gcgcggcgcg
      481 cgcggccatc gcgcgggtcg tctcgatggg gaactcgacc accgcgacat tggcaatgcg
      541 ctccatcgcc gccgcgttct gcttccacca cctttccgaa ccggtcccgt acgtgtacac
      601 ggcaacgcga tcggcgcggc cggatgcctt tcgcacgtcg cgttcgtcgg gcaggccgac
      661 gtcgatccaa agatcgatgg cgcccgtgcg gtcggcgagg cagaggtccg cctcgccctc
      721 gctggacagc ccgcgcccga acgcgaggcg ctcgtccgcg ttcaacgcga aagcgagcag
      781 gcgcaccatc atccgttcgt ccgtttccga cggatggcgc gcgatggtca gcgcgtgggt
      841 ctcgaaatgt ccgcggtcca ggtcggaaac cgtgagctcg accttgaaga tggtcgactt
      901 gagggccatc ggggccgcgc ctacttcttc gcggccagcc ccttcttcag catttcggcc
      961 acggtttcgt tgaggctggc gcccgaggct tcggcgcgcg cctgcaattc cttgatgagg
     1021 tcgccatgaa gcttgcacgc gaagggcacg aggccggcgg cggcatcgag cttgcgctgc
     1081 tccttgcggt cggtgacatg ggcgacgccc ctgccgtagc ggtcggacac gccgccgcgg
     1141 cgggcttcgc cctcgatctt caggccctgg ttcttgtgca gctcggtgag tttcatggca
     1201 atcgcgctca gggggatgga aagcgccatt ttacgcggcg caggggcggg ggcagtagaa
     1261 ttgcatgttg tccatgagga attgcgccat gaccgcacgc cccgacatcc ccgccttcgc
     1321 cgccggcccg ctcgcggcgc gcatcgaaag gcagtgggac tcggacatca tcccgcagct
     1381 cgtcgactac atccgcattc ccgccaggag tccgcacttc gacgcggact gggcgaagaa
     1441 cggacacatc gaggccgccg tccgccaggc cgagaagtgg gtcacggccc aggccgtcaa
     1501 gggcctgtcg ctcgagatcg tgcgcctcgg ggagcgcacg ccggtgctct tcttcgacat
     1561 ccccgccacg ggcggcctgg cgggcgagcg cacggtgctg ttctacggcc acctcgacaa
     1621 gcagcccgag atgacgggct ggcgcgacgg attgggcccg tggacacccg tcatcgagga
     1681 cggcaagctc tacggccgcg gcggggcgga cgacggctat gcggtcttcg ccgcgctctc
     1741 cgccgtgatg gcgctggatg cagaaggcgt cgcgcggccg cgctgcgtgg gcatcatcga
     1801 aacgtgcgag gaaagcggca gctacgacct gcccgcctac ctccatgcgc tggctccccg
     1861 catgggcgac gtgcagctcg tcgtcgcgct cgattccggg gccggcaatt tcgagcagct
     1921 ctgggtcacc acgtcgctgc gcggcctggt gaacggcacg ctcgacgtga ggattctcga
     1981 ggagggcgtg cattccggcg acgccggcgg cgtggtgccg tcctccttcc gcatcgcgag
     2041 gcgcatcctc gaccgcatcg acgattcgag caccggcgcg gtgttgcccg ttgccttcca
     2101 ctgtgacatc cccgccgacc ggctcgagca ggcgcggcag gccgcccgga tcctgggcga
     2161 cgcgacgtgg aagcggtttc cctgggtctc atgctgcgac gacgccggcg cgaaccgggt
     2221 gttcgccgag ccggtgacga aggatcccgt ggagctgatc ctcaaccgca cgtggcgcgc
     2281 ggcgctcgcg gtcacgggcg ccgacggcct gccgcccacg aacttcgcgg gcaacgtgca
     2341 gcgcccgttc accagcctga agctctcgct gcgcctgccg ccatccgtcg atgcggcgag
     2401 tgcgagcgag atcttcaagc aggcgctgga ggccgatccg cctcatggcg cgcgcgtgtc
     2461 cttcacttgc gattcggcgg cgacgggatg gaatgcgccc agcctgacgc cgtggctgtc
     2521 ggccgcgctc gacgccgcct cgcaggcaag cttcggcaag cccgcggcct acatggggga
     2581 gggcggcacc attcccttca tgggcatgct gggcgtcaag ttcccgaacg cgcagatact
     2641 cgtcaccggc gtgctgggcc cgaagtcgaa cgcgcacggc cccaacgaat tcctgcacct
     2701 gtcctacgcc aagaaggtga cggtggccac cgcgatggtc atcgcggcgg tgcgttgatg
     2761 cgccggcgaa cggtcgctgc cggactcatc ctggttcttg cagccgcgct cggcggctgg
     2821 tggctgtcgc ggcaggacaa ggcgccgcaa gccgccgcca agggaggtgg ggtcgccgtg
     2881 ccggtgacgg tggccacggt cgagcgccgc gacgtgcccg tgcggctgct ctccaacggc
     2941 acggtggtga cgctgcagtc ggtggacatc cgcgcgcaga tcacgagcac ggttcgcgaa
     3001 gtgcacatcc gcgaggggca gaacgtcgcc aagggcgacc ttctcgtttc cctcgattcc
     3061 cgcgcggaag aggcgaacct gaagaaagcc gaggcgcagg tggagaagga ccgcgccgac
     3121 ctggccaacg ggctgcgcac gctggagcgc cagcgccagc tcttcgcgca gaaattcatc
     3181 tcgcagtccg cgctcgacgc ggcgcagaac caggtcgaga c
//
LOCUS       DASUEV010000333         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1359896, whole genome shotgun sequence.
ACCESSION   DASUEV010000333 DASUEV010000000
VERSION     DASUEV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359896"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..676
                     /gene="rplE"
                     /locus_tag="VFV55_10545"
     CDS             176..676
                     /gene="rplE"
                     /locus_tag="VFV55_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX4944783.1"
                     /translation="MKQFGYKTVMQTPRIEKITLNMGVGEAVADKKILENASGDLVKI
                     GGQKPVVTKSRKSIASFKVRAGYPVGCMVTLRSRRMYEFLDRLVTIALPRVRDFRGVS
                     GRAFDGRGNYSMGIKEQIIFPEIDYDKVDALRGLNICITTTAKSDAEARALLAAFRFP
                     FRTERA"
     gene            676..981
                     /gene="rpsN"
                     /locus_tag="VFV55_10550"
     CDS             676..981
                     /gene="rpsN"
                     /locus_tag="VFV55_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX4944784.1"
                     /translation="MAKLALINREAKRRATVKKFAEKRKQLFAVIKDSKATDDARDEA
                     LAKLQAMPRNASPVRLRNRCAITGRPRGTFRKFGLGRNKIREIAMRGEIPGMIKASW"
     gene            992..1387
                     /gene="rpsH"
                     /locus_tag="VFV55_10555"
     CDS             992..1387
                     /gene="rpsH"
                     /locus_tag="VFV55_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX4944785.1"
                     /translation="MSMSDPIADMLTRIRNAQATDKVSVAIPASKVKLAIAKVLKDEG
                     YIEDFAQRDVEGKSVLEIGLKYYAGKPVIEKIERISRPGLRIYKGRDDIPRVLNGLGV
                     AIVSTSRGVMTDRRARETGVGGEVLCIVV"
     gene            1399..1932
                     /gene="rplF"
                     /locus_tag="VFV55_10560"
     CDS             1399..1932
                     /gene="rplF"
                     /locus_tag="VFV55_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX4944786.1"
                     /translation="MSRIAKNPVVIPEKVEVALGESDLTVKGPLGTLKQSLSSDVVVK
                     VEGAHVTFAAANGSIRANAMSGTMRALVANMVHGVKQGFEKKLTLVGVGYKAQAQGNK
                     LNLALGFSHPIVHMLPEGVKAETPTQTEILIKGVDRQKVGQVAAEIRAYKSPEPYKGK
                     GVRYANETVVIKETKKK"
     gene            1966..2322
                     /gene="rplR"
                     /locus_tag="VFV55_10565"
     CDS             1966..2322
                     /gene="rplR"
                     /locus_tag="VFV55_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX4944787.1"
                     /translation="MTDKKPSRLRRAAKTRARIERQEAVRLTVHRTNQHIYAQVVAAG
                     GGKVLASASTVEKEVRAQQVNGGNTQAAALVGKRIAEKAKAAGVSEVAFDRAGFSYHG
                     RVKALAEAAREGGLKF"
     gene            2354..2863
                     /gene="rpsE"
                     /locus_tag="VFV55_10570"
     CDS             2354..2863
                     /gene="rpsE"
                     /locus_tag="VFV55_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX4944788.1"
                     /translation="MAKYEQNDERSDGLREKMISVNRVTKVVKGGRILGFAALTVVGD
                     GDGSIGMGKGKSREVPVAVQKAMEQARRNMVKVALKGGTIHHEVLGRHGATRVFMQPA
                     AEGNGIKAGGAMRAVFEVMGVTNISAKCHGSTNPYNVVRATLDGLLQQHRPSEIAAKR
                     GKKVEEIVG"
     gene            2878..3063
                     /gene="rpmD"
                     /locus_tag="VFV55_10575"
     CDS             2878..3063
                     /gene="rpmD"
                     /locus_tag="VFV55_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX4944789.1"
                     /translation="MTSPKKIKVTQVKGLRGALSAHKASVRGLGLRRIRHTVELDDTP
                     SVRGMINQVSYLVKVES"
     gene            3068..3502
                     /gene="rplO"
                     /locus_tag="VFV55_10580"
     CDS             3068..3502
                     /gene="rplO"
                     /locus_tag="VFV55_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX4944790.1"
                     /translation="MELNTLKPAAGSKKARRRVGRGIGSGLGKTAGRGHKGQKSRAGG
                     FHKVGFEGGQMPLQRRMPKRGFAAVTIESAVEVRLGDLRKVEGDTVDLVALKAAGIVP
                     GSVKRAKIILAGKVEKALKVQGIGVTKGAKAAIEAAGGSVAG"
     gene            3552..>4586
                     /gene="secY"
                     /locus_tag="VFV55_10585"
     CDS             3552..>4586
                     /gene="secY"
                     /locus_tag="VFV55_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX4944791.1"
                     /translation="MGDLKKRLWFLVGALVVYRVGTFIPVPGIDPAELAKLFQQTKGG
                     ILDMFNMFSGGALSRFSIFALGIMPYISASIIMQLLTVVSPQLEALKKEGEAGRRKIT
                     QYTRYGTVVLAAFQALGISVALEAQPGLVIDAGLAFRLVTVVTLMTGTLFLMWLGEQI
                     TERGLGNGISMIIFAGIVAGLPSAMGGTLELVRTGAMTIFVALIIFALVVGVTYVVVF
                     VEKGQRRILVNYAKRQVGNKLYGGQSSFLPLKLNMSGVIPPIFASSIILFPATLASWF
                     GTNERMTWLRDLSAALSPGQALYIVLYASAIIFFCFFYTALVFNSKETADNLKKSGAF
                     VPGIRPGDQTA"
BASE COUNT          889 a         1526 c         1511 g          660 t
ORIGIN      
        1 agatcagaag tccgcgaaga ccgagaagtc cgcgaagccc gacaagaccg cgaaggccga
       61 aaagtccgcg aagcccgaca agaccgcgaa gaccgaaaag tccgcgaagc cgcaggcacc
      121 ctcgcagccg ccgcgcctgc agacgctcta ccgcgagacc gtcgtcccgg cgctgatgaa
      181 gcagttcggc tacaagacgg tcatgcagac gccgcgcatc gagaagatca cgctcaacat
      241 gggcgtgggt gaggccgtcg cggacaagaa gatcctcgag aacgcctcgg gcgacctcgt
      301 gaagatcggt ggccagaagc ccgtcgtcac caagtcgcgc aagtccatcg cgagcttcaa
      361 ggtcagggcc ggctacccag tcggctgcat ggtgacgctg cgctcgcgcc gcatgtacga
      421 attcctcgac cggctggtca cgatcgcgct gccgcgcgtg cgcgacttcc gcggggtgtc
      481 cggccgggca ttcgacggcc gcggcaacta cagcatgggc atcaaggagc agatcatctt
      541 ccccgagatc gactacgaca aggtcgacgc gcttcggggc ctgaacatct gcatcaccac
      601 caccgcgaag tcggatgcgg aggcgagggc actccttgcc gccttccgct tcccgttcag
      661 aaccgagagg gcgtaatggc aaagctcgca ctcatcaacc gcgaggcgaa gcggcgcgct
      721 accgtgaaga agttcgcgga aaagcgcaag cagctcttcg cagtcatcaa ggattccaag
      781 gcgaccgacg acgcgcgcga cgaggcgctg gcgaagctgc aggcgatgcc gcgcaatgct
      841 tcccccgtgc gcctgcgcaa ccgctgcgcg atcacgggcc gcccccgcgg cacgttccgc
      901 aagttcggtc tcgggcgcaa caagatccgc gagatcgcga tgcgcggcga gatccccggc
      961 atgatcaagg cgagctggta ggagacccac gatgagcatg agtgatccca tcgcggacat
     1021 gctgacccgg atccgcaacg cgcaggcgac ggacaaggtg agcgtcgcga tcccggcttc
     1081 caaggtgaag ctcgcgatcg ccaaggtcct gaaggacgaa ggctacatcg aggacttcgc
     1141 gcagcgcgac gtcgaaggca agagcgtcct cgagatcggc ctcaagtact acgcgggcaa
     1201 gccggtgatc gagaagatcg agcgcatctc gcgccccggg ctgcgcatct acaagggtcg
     1261 cgacgacatt ccgcgcgtgc tgaacggcct cggcgtggcg atcgtctcca cctcccgcgg
     1321 cgtgatgacc gaccggcgcg cccgcgaaac gggcgtgggc ggcgaagtcc tctgcatcgt
     1381 ggtttaagga agccagccat gtcgcgcatc gccaagaatc ccgtcgtcat tcccgagaag
     1441 gtcgaagtcg cccttggcga gtccgacctc acggtgaagg gccccctcgg cacgctgaag
     1501 cagtccctgt ccagcgacgt cgtcgtgaag gtcgagggcg cgcacgtcac gttcgccgcg
     1561 gccaacggct cgatcagggc aaacgccatg tccggcacca tgcgcgcgct cgtcgccaac
     1621 atggtccacg gcgtgaagca gggcttcgag aagaagctca cgctggtcgg ggtgggctac
     1681 aaggcgcagg cccagggcaa caagctcaac ctcgcgctgg gcttctccca cccgatcgtg
     1741 cacatgctgc ccgaaggcgt gaaggccgaa accccgaccc agaccgagat cctgatcaag
     1801 ggcgtcgacc gccagaaggt cgggcaggta gccgcggaga tccgcgccta caagtccccg
     1861 gagccgtaca agggcaaggg cgttcgctac gccaacgaga ccgtggtgat caaggaaacg
     1921 aagaagaagt aacgcggctg acgcgttgct acggagaatt cgatcatgac cgacaagaaa
     1981 ccctcgagac tgcgccgtgc cgcgaagacg cgtgcgcgca tcgaacgcca ggaagccgta
     2041 cgcctcaccg tgcaccgcac caaccagcac atctatgcgc aggtggttgc cgccggcggc
     2101 gggaaggtcc tcgcctcggc ctcgaccgtc gagaaggaag tgcgcgccca gcaggtgaac
     2161 ggcggcaaca cgcaggccgc ggccctcgtc ggcaagcgca tcgcggagaa ggcgaaggcc
     2221 gccggcgtca gcgaggtcgc attcgaccgc gccggattca gctaccacgg ccgcgtgaag
     2281 gcgctcgccg aggccgcccg cgagggcggg ctgaagttct agtccgccca ggcagactca
     2341 caggaaaacg aacatggcga aatacgaaca gaacgacgag cgcagcgacg gcctccggga
     2401 gaagatgatc tcggtgaacc gggtgaccaa ggtcgtcaag ggcggacgca tcctcggctt
     2461 cgcggcgctc acggtggtgg gcgacggcga cggctccatc ggcatgggca agggcaagtc
     2521 ccgcgaggtc ccggtggccg tgcagaaggc gatggaacag gcgcgccgca acatggtgaa
     2581 ggtcgccctc aagggtggca ccatccacca cgaggtgctc ggccgccacg gcgcgacgcg
     2641 agtgttcatg cagcccgccg cggaaggcaa cggcatcaag gccggaggcg ccatgcgcgc
     2701 ggtgttcgag gtgatgggcg tgaccaacat ctcggccaag tgccacggtt ccacgaatcc
     2761 ctacaacgtc gtgcgcgcca cgctggacgg gctgctgcag cagcaccgcc cctccgagat
     2821 cgcggccaag cgcggcaaga aggtcgaaga gatcgtcggc tgaggaaaga ccccgagatg
     2881 acttccccca agaaaatcaa ggtgacccag gtcaagggcc ttcgcggcgc gctgagcgcg
     2941 cacaaggcat cggtgcgcgg cctcggcctg cgccgcatcc ggcacaccgt ggagctggac
     3001 gatacgccct cggtccgcgg catgatcaac caggtgagct acctcgtgaa agtcgagtcg
     3061 taaagccatg gaactcaaca cgctcaagcc cgcagcgggc tcgaagaagg cccggcgccg
     3121 cgtcggccgc ggcatcggct ccggcctcgg caagaccgcg ggacgcggcc acaagggcca
     3181 gaagtcgcgt gccggcggct tccacaaggt cggcttcgag ggcggccaga tgccgctgca
     3241 gcgccgcatg cccaagcgcg gattcgccgc ggtcaccatc gagtcggccg tggaagtgcg
     3301 cctcggcgac ctgcgcaagg tcgagggcga caccgtggac ctcgttgcgc tcaaggcggc
     3361 cggcatcgtc cccggttccg tgaagcgggc caagatcatc ctcgcgggca aggtcgagaa
     3421 ggccctcaag gttcagggca tcggcgtcac gaagggcgcg aaggcggcca tcgaggccgc
     3481 cggcggctcc gtcgcgggct gagcggaagg atatcgcctt gtcggccaac cccctcgcga
     3541 ctctcggaaa gatgggcgac ctgaaaaagc gcctgtggtt tctcgtgggc gccctggtcg
     3601 tctatcgcgt cgggacattc atcccggtgc cgggcatcga cccggcggaa ctcgcgaagc
     3661 tcttccagca gaccaagggc ggcatcctgg acatgttcaa catgttctcg gggggggccc
     3721 tttcccgctt ctcgatcttc gcgctgggga tcatgcccta catctccgcg tcgatcatca
     3781 tgcagctgct gacggtcgtc tctccgcagc tcgaggcgct gaagaaggag ggcgaggccg
     3841 gccgccgcaa gatcacgcaa tacacgcgct acggcaccgt ggtgctcgct gccttccagg
     3901 cgctcggcat ttccgtcgcc ctcgaggcgc agccgggtct cgtcatcgac gcgggcctcg
     3961 cgttccggct cgtgacggtc gtcaccctca tgacgggcac cctgtttctc atgtggctgg
     4021 gcgagcagat caccgagcgc ggcctgggca acggcatctc gatgatcatc ttcgcgggca
     4081 tcgtcgcggg tctgccgagc gcgatgggcg gcacgctcga actcgtgcgc accggtgcca
     4141 tgacgatctt cgtcgcgctg atcatcttcg cgctggtggt aggcgtgacc tacgtcgtgg
     4201 tgttcgtcga gaagggccag cgccgcatcc tggtgaacta cgcgaagcgc caggtcggca
     4261 acaagctcta cggcggccag agctcgttcc tcccgctcaa gctaaacatg tcgggcgtca
     4321 tcccgccgat cttcgcctcg tcgatcatcc tcttcccggc cacgctcgcg agctggttcg
     4381 ggacgaacga gcggatgacc tggctcaggg acctctccgc ggcgctatcc cccgggcagg
     4441 ccctctacat cgtgctctac gcctcggcga tcatcttctt ctgcttcttc tacacggcgc
     4501 tcgtcttcaa ctccaaggag acggcggaca acctgaagaa gagcggcgcc ttcgtgcccg
     4561 gcatccggcc cggcgaccag acggcg
//
LOCUS       DASUEV010000334        15514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_789759, whole genome shotgun sequence.
ACCESSION   DASUEV010000334 DASUEV010000000
VERSION     DASUEV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789759"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..976)
                     /locus_tag="VFV55_10590"
     CDS             complement(11..976)
                     /locus_tag="VFV55_10590"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX4944792.1"
                     /translation="MPLRMPAPDAMSVFLRPKVLLEFDVALNAIAYMPLGALACLILR
                     ASNSPRAAQARAVAWCAALSLAMEALQFFVPNRVASIYDLAANAAGAFAGTLVFAEPL
                     HELATHPLAQARERLVAAGGWGDAGLVLVVLWLLAQLNPAIPFFEAGNIVGNGDPQPG
                     DFLVTAGSVALSVAGFGFFVSVVLKGPGGALRWTLLLLTVALWCKFAMASVMLKPHLS
                     AGWVTEGRIVGLAAGLLAFVPLRRLGRTTRIYFGIVLMLAGALLAKIFGAYSALDDFL
                     RLFSWPYGQLATFATLTRFIHEAWPVLALAWLCALFVWRRVEPIQ"
     gene            complement(1047..1568)
                     /locus_tag="VFV55_10595"
     CDS             complement(1047..1568)
                     /locus_tag="VFV55_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX4944793.1"
                     /translation="MIYELEGRKPVLEGDNFVAPDAAVIGSVVMKKGANVWWGVTIRG
                     DNDVITIGEGVNVQDGSVIHTDAGIPVTLEKNVSIGHMVMLHGCTIGENSLIGIGAII
                     LNRAVVGKNCLIGAGALVPEGKHIPDGSLVLGMPGKVVRHLSAEEIANNTWIAEHYVE
                     RSGIYRKGLKPVG"
     gene            1785..4649
                     /locus_tag="VFV55_10600"
     CDS             1785..4649
                     /locus_tag="VFV55_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX4944794.1"
                     /translation="MKRLLSSMVPSLRVVSTGFALLLPLPVPAQHSADRIEKVEKVEV
                     TGSRIATPDLANSSPVTVVKAEDIALEGYPSLDLFLNNLPQVFADQGNRISNGATGTA
                     AVSLRGIGAIRTLVLVNGRRLPAGSPHYLAPDLNQIPAPLVSRVEVLTGGASAVYGSD
                     AVAGVVNFILNDRFEGVQGEIGHSFYNHRQKSPIADVVRASNFDVPGDRARDGETTHV
                     SLLLGGNFAQERGNATVFFSHLKTEAVLEGDRDFSACSLTASDAGFSCGGSFTGYPGR
                     FVNLADGRSWTVANAAGGVRPWDPATDLYNFAPLNYFQRPAERYGFNASAHYALSADA
                     RVYAEFGFHDDHTVAQIAPSGLFGVEATIPFGNPLLSADWRTNLGLTAPGTSAQLLIL
                     RRNVEGGGRQDDIRHTSYREVIGVKGTLPGNWDFDLFQQVGRVVYQATYKNDFSISRG
                     LRALDVVVDPATGAPVCASVLNGSDPNCVPYNIWSLGRVTPEALAYLQTPGFQKGFTS
                     QQVAGATLTGDLGRYGIRFPGSRTGVETAFGAERRTEKLELETDSAFSTGDLAGQGGS
                     IIGVAGAYTVKDFFGEIRAPLTGALSMSASYRYSDYSTGKTTHTYGLGFDFAPVNRVR
                     LRGTYQQAVRAPDIVALFAPQAVGSEQIGDDCAGPAPSRSLAECQRTGVTPAQYGHIL
                     ESPSGFYPVVFGGNADLKPETAKTWTLGVVFTPTAHLKATIDYFSIALSDTILRFFPT
                     ISLSQCLDTGDPRFCSLITRDPLRGTLWLPPARIVATDQNIGRGATSGMDVSLSYTHR
                     LDGNGTVDIGALGSYLRSLELEPYAGASAFDCAGLYGGGCGHPSPVWRHRLRATWNTP
                     WDFNIAATWRYTSSLRHAGTSSNPLLASDIQEVIRKLKAVNYFDVAGSWSLNRHLTLR
                     AGVNNLLDRDPPVALTEASTFAQWYDSLGRYLFVSLTARF"
     gene            complement(4794..5546)
                     /locus_tag="VFV55_10605"
     CDS             complement(4794..5546)
                     /locus_tag="VFV55_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX4944795.1"
                     /translation="MPTAILAEDEPILRTQLESKLNKLWPELEIIASVEDGAAALEAL
                     EERAPDFMFLDIQMPEMTGVEVARHVGTRAHVVFVTAYDQYAIQAFETGAVDYILKPY
                     TDERLAVSVERLKGKLASPPPDLNAIVSQLATQLRGGAAREKLQWIKASFGQNLRLIP
                     VSDVLFFQSDEKYTRVVTAEAEALIKTPIRELLDGLDSEVFWQIHRSTLVNVNAIAGV
                     TRDFRGQAHVKIRGKDETLTVSRIYSHLFRQM"
     gene            complement(5556..7106)
                     /locus_tag="VFV55_10610"
     CDS             complement(5556..7106)
                     /locus_tag="VFV55_10610"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX4944796.1"
                     /translation="MINLAMGLERGAQLHPGKPAVFFADRVFTYGQLDAAANRVANGL
                     RALGIGRGDNVALACPNVPYFPIVYYGILKAGATVVPVSVLSKAPEIAYHLEDSRAKA
                     FFCFEGSAELPLGKEGHAGFTQCGACPHFFMITADPAKPCAIDGVRTLGAFMQAQPDT
                     GTSADTDANDAAVILYTSGTTGKPKGAELTHANMAMNAFVSGMITQMVAADCHLVALP
                     LFHSFGQSVQMNAGIWRGCSMVLLPRFDPDAALAAMQKHKVTIFAGVPTMYQAILNLA
                     GAEERHDLKSIAANLRVGVSGGAAMPVEVMRRFEERFGIAILEGYGLSETSPVATFSF
                     LDFPRVPGSVGKPVWGTQVRVVDESGAPVPTGQPGEIVIRGHNVMKGYFRRPEATAQA
                     IRDGWFHTGDIGRVDPEGNFYIVDRLKDMIIRGGFNVYPREIEEVMMTHEAIAQVAVI
                     GVPHETHGEEVKAVVVLRAGKDATPEAIVAWCKERMAAYKYPRIVQIVESLPMTATGK
                     ILKRALKQ"
     gene            complement(7121..8269)
                     /locus_tag="VFV55_10615"
     CDS             complement(7121..8269)
                     /locus_tag="VFV55_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX4944797.1"
                     /translation="MPFAIPRTLFEPEHEQFRDALRRFVEIEVLPNLGRYEEQGFIDR
                     EIWRKGGAAGFLCPTMPEEYGGAGADRRFSVVVFEEPARMGAISLLGWSLHSDIVAPY
                     ILHYGAEALKRQYLPKMATGEMIGAIAMTEPDAGSDLQRIRTSAVRDGDHYVLNGAKT
                     FITNGSHCDLVIVAAKTDPSQGAGGTSLFVVDTSMPGFTKGRRLAKVGLKSQDTGELF
                     FADVRVPAVNLLGEEGKGFAYLMQELPWERLQIAISAIAMAEGVLDGTLAYCRDRKAF
                     GKTILEFQNTRFSLAESKTELQVARVFVDRCIELVLAGTLDAATASMAKYWCTDLQCR
                     LIDACLQLHGGNGYMWEYPVARAWADARASRIYGGTNEIMKELISRSL"
     gene            complement(8318..10471)
                     /locus_tag="VFV55_10620"
     CDS             complement(8318..10471)
                     /locus_tag="VFV55_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4944798.1"
                     /translation="MKPFSDSIHRTPWWALVIGGFAIIVALAVFVTPFHLIEYRKEGA
                     TPEENRAIKREIDNTFAEGAVDVARSAILALRGATSDPLRRAELDRAIEELDGARAEL
                     RDAGREVLRAKREAIDTAREATREAASAIEEARRNAEQALKAAGLENDKVKAALEQSL
                     KSAREAEDEAKKALETDAKGAETRKRIVIGMGAGKDRPLLDIDVSKADPGERPHVEIH
                     APGATVKGPRGSITLDLGNMPEGMTPTPPTPPTVALPPLPPEIRAEIRRNVGDDLRKI
                     GIGAGLVLLFIPLFILTVVAKFFIDRSRAAQRMADLKRKEAEYHRMSQQVTEAKLQAL
                     QAQVEPHFLYNTLANVQALTEVDPAQANTMVGHLIQYLRSALPKMRESVSSVGQEIEL
                     VRAYLNILQMRMGKRLSFEIAVPESLLATPFPPLMVPSLVENAIKHGLEPLREGGSVL
                     ITAQAQDGQLRLVVSDTGRGFGETVGTGVGLANIRERLAGLYGDKARLTLESNSPSGV
                     IATIEVPLNGLRAATAVPPGAAATNSFAWPGNDPGSRGEAQPAAAKSAAGKVLSAVGT
                     AERTWRKTLSFAFVALVVVAAVVSGLGVFGVLTGLVPVQVGTETLTGTSGALLGAAGI
                     AIGFAAVVLVLAIVAALIYGLGWLLAGLAIFIPVVILIALSPALAPVVLVVLLIWWIL
                     RKKKDPQPVAAPKVEPVMKAQATQAADPGKEPPAV"
     gene            complement(10794..11549)
                     /locus_tag="VFV55_10625"
     CDS             complement(10794..11549)
                     /locus_tag="VFV55_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX4944799.1"
                     /translation="MGIPTAVIADDEPLLRAQLRSRLGKLWPELAIVHEMENGRDIEQ
                     VIAAHAPRLFFLDIHMPGVNGLEAARTIGKRAHVVFVTAFDQYAIEAFERGAIDYVLK
                     PFNEERLASTVERLRERLSDAPPAMEELVERLAGRLVPRAAESLRWIKASVGQSVRLI
                     PVEEVLFFQSDEKYTRVVTADSETLIRKPIKELLEELDAEKFWQIHRATIVNVEAIAA
                     VRRGLKDQAEITLKGHRETLIVSRNFTHLFKQM"
     gene            complement(11562..12644)
                     /locus_tag="VFV55_10630"
     CDS             complement(11562..12644)
                     /locus_tag="VFV55_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4944800.1"
                     /translation="MNRYAYLFEGLTLRRVLLVSFLCVWAAAATVWFFVNTYLDLLVS
                     SLCVGYTSMVLFTVATNLRQRKLPRWAVQVLSIVVGSFLGTMLAGIVKGRDFATMFSE
                     RFYGVLITMGLGIGFGAMAVWVYVFRERESRHAAELARAQAERAQLEKNVLQAQLALM
                     QAQVEPHFLFNTLANVQHLVETDPASASRMLESLIQYLRAALPRMREDATTVGREADM
                     ARAFLDIHRMRMGSRLDYVLDVPEALRCRPFPPMMLISLVENAIKHGVDPCCEAGTIT
                     IRAREEGGKLRFSVADTGEGISPRKGTGVGLVNIRERLKALHGSGARLLMEENTPHGV
                     IATIEVDAFSAIEVPLPASSVAAPCV"
     gene            complement(12681..12851)
                     /locus_tag="VFV55_10635"
     CDS             complement(12681..12851)
                     /locus_tag="VFV55_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944801.1"
                     /translation="MSRPANLMTLPWTIRLRSGDAMRQPQQRSAALPWSSPRPGIAPR
                     WKPRDRVSAAGN"
     gene            complement(13070..13198)
                     /locus_tag="VFV55_10640"
     CDS             complement(13070..13198)
                     /locus_tag="VFV55_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944802.1"
                     /translation="MKLIERIISSHALGVSTAITAIVAGWFALTFAITVGGLVVGA"
     gene            complement(13209..13502)
                     /locus_tag="VFV55_10645"
     CDS             complement(13209..13502)
                     /locus_tag="VFV55_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944803.1"
                     /translation="MSKLEKLDLMGVASLAVVTAALLVGWMVTSYHSAAPQARHAAQE
                     QAVTLTDDGRMRLVVTAARDDAGTPNAAPTRTASVRPAGGPSLEVALPFAFRP"
     gene            complement(13656..15077)
                     /locus_tag="VFV55_10650"
     CDS             complement(13656..15077)
                     /locus_tag="VFV55_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX4944804.1"
                     /translation="MLLWFVIIYWIISVAIGIYAAKFVHNSKDFAVAGRAMPMAVVTA
                     TVFATWFGSETVLGIPATFLKEGFSGVIADPFGSSLCLVLVGLFFARPLYRMNLLTIG
                     DYYRKRYSRPVEMLTTLCIVISYLGWVGAQIKALGLVFNVVSEGAITQQWGMIIGAST
                     VLVYTMLGGMFSVAITDFIQMIIIVLGMLYIGWEVSGLAGGVGTVIAHAAANDKFHIL
                     PAPELKDILWFLAAWITMMLGSIPQQDVFQRVQSARNENIAARSSILGGVLYFGFAFI
                     PMFLAYSATLIDPKMVEANLAGDSQLILPNLILSPVVPIVAQIMFFGALLSAIKSCAS
                     ATLLAPSVSFSENILRPLLPHIGDQRFLHLMRVVIVGFTVVVTAVALSTEASIYKMVE
                     NAYKVTLVGAFVPLVCGLYWKRATNLGATLAIIFGLVTWIGFERYGADGTMPPQLAGL
                     VASFGGMIVGSLAMRSLQREAAV"
     gene            complement(15201..>15514)
                     /locus_tag="VFV55_10655"
     CDS             complement(15201..>15514)
                     /locus_tag="VFV55_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944805.1"
                     /translation="VEGCNFIGVLLRVYVPLAKPTYLAYGLVSVSYHWNNFLWPLIIT
                     NSVNTRPLTVGLAIFGAPESGVDWAVISAGTLMAVSPLLVAFLLFQRQFMQSFMQAGI
                     K"
BASE COUNT         2655 a         5138 c         5235 g         2486 t
ORIGIN      
        1 gggggagcgg tcattgtatc ggctcgacgc gccgccacac gaacagcgcg cacagccacg
       61 cgagcgccag caccggccag gcctcgtgga tgaagcgggt cagcgtggcg aaggtcgcga
      121 gctggccgta gggccagctg aagaggcgca ggaagtcgtc cagggcactg taggctccga
      181 agatcttggc gagcagcgcg cccgcgagca tgaggacgat gccgaagtag atgcgcgtcg
      241 tccttccgag ccggcgcaac ggcacgaagg cgaggagacc tgccgcgagc cccacgatgc
      301 gtccttccgt cacccatccc gcggagagat gcggcttgag catcaccgag gccatggcga
      361 acttgcacca cagcgccacg gtaaggagga gaagcgtcca gcgaagcgcc ccgcccggcc
      421 ccttgagaac gaccgacacg aagaacccga aacccgcgac cgagagcgcg acggagccgg
      481 cagtgaccag gaaatctccc ggctgcgggt caccattgcc aacgatgttg cccgcctcga
      541 agaagggtat cgcggggttc agctgcgcca gcagccagag cacgacgagc accagccccg
      601 cgtcgcccca gccccccgcg gcgacaaggc gctccctcgc ctgggccagc gggtgcgtgg
      661 cgagttcgtg cagcggctcg gcgaagacga gcgtgccggc aaacgcgccg gccgcattcg
      721 cggccaggtc gtagatcgag gcgacgcggt tcggcacgaa gaactgcagg gcctccatcg
      781 cgaggctcaa ggccgcgcac caggccaccg ccctcgcctg cgcggcccgc ggcgagttcg
      841 aagcgcgcag gatcaggcag gcgagcgcgc ccagcggcat gtaggcgatc gcgttgagcg
      901 cgacatcgaa ttccagcagc actttcgggc gcaggaacac cgacatcgcg tcgggcgccg
      961 gcatgcgcag gggcacgaac gggtacaggc tcgcgtaggc gacgagcagc gcccagaccg
     1021 cgagcgcccc gcgatggcgg gcggcgctag ccgacaggct tcaacccctt gcggtagatg
     1081 cccgagcgct cgacgtagtg ctccgcgatc caggtgttgt tggcgatttc ctcggcggag
     1141 aggtggcgca cgaccttgcc cggcatgccc aggaccagcg agccgtcggg gatgtgcttg
     1201 ccctcgggaa cgagcgcgcc cgcgccgatg aggcagttct tcccgacgac ggcgcggttg
     1261 aggatgatcg cgccgatgcc gatgagggaa ttctcgccga tggtgcagcc gtgcagcatc
     1321 accatgtggc cgatgctcac gttcttctcc agcgtgacgg gaatgccggc gtcggtgtgg
     1381 atcaccgagc catcctgcac gttcaccccc tcgccgatcg tgatcacgtc gttgtcgccg
     1441 cggatggtga cgccccacca cacgttggcg cccttcttca tcaccacgga gccgatcacg
     1501 gcggcatcgg gggcgacgaa gttgtcgcct tcgaggacgg gcttgcggcc ttccagttcg
     1561 tagatcacgg cggcgcctgg cgaaggatcg gatcgcggca ttttcgcacg gacgcttcgc
     1621 ttgccgggaa tccggggctc cggaaagcaa gcccgatgcc tttcgggggg cacgtcttgc
     1681 gtgaatattc cccttgcgtc cgtgtgcaat cggatcagta tggctcgacg agaggcgcgc
     1741 atggcgggcc ctcccgattg cggcagtcga tccggaggtg ccccatgaag cggctactct
     1801 cctcgatggt tccgtcattg cgcgtcgtat ccaccggatt cgcgctcctg cttccgctgc
     1861 cggttccggc ccagcactct gccgaccgga tcgaaaaggt cgagaaggtc gaggtcaccg
     1921 gctcgcgcat cgcgacgccc gacctcgcga acagttcgcc ggtcacggtc gtcaaggccg
     1981 aggacatcgc cctggagggc tacccgtccc tcgatctctt cctcaacaac ctgccccagg
     2041 tcttcgccga ccagggcaac cggatatcca atggcgccac cggcacggcg gccgtcagcc
     2101 ttcgcggcat aggcgcgatc cgcaccctcg tgctggtgaa tggccgccgc cttcccgcgg
     2161 gaagccccca ttaccttgcg ccggacctga accagatacc ggcaccgctg gtctcgcgcg
     2221 tggaagtgct caccggcggc gcctcggcag tctatggctc cgatgccgtg gcgggcgtgg
     2281 tgaacttcat cctcaacgac cggttcgagg gcgtccaggg cgagatcggc cacagcttct
     2341 acaaccacag gcagaagagc ccgatcgccg acgtggtccg ggcgagcaac ttcgacgttc
     2401 cgggcgacag ggcgagggac ggggaaacga cccacgtcag cctgctgctc ggcggaaact
     2461 tcgcgcagga gcggggcaac gcgaccgtat tcttcagcca cctgaagacc gaggcggtgc
     2521 tggaaggcga tcgcgatttc agcgcctgtt cgctcacggc aagcgacgcg gggttttcct
     2581 gcgggggctc gtttacgggc tatcccgggc gcttcgtgaa tctcgcggat ggccggtcat
     2641 ggacggtcgc caacgccgcc ggaggcgttc ggccgtggga ccccgcaacc gatctctaca
     2701 acttcgcgcc gctgaactat ttccagcgac cggcggagcg ttacggcttc aacgcctccg
     2761 cccactacgc cctctcggcc gacgccaggg tgtacgcgga gttcggcttc cacgacgacc
     2821 ataccgtggc gcagatcgca ccgagcggat tgttcggcgt ggaggcgacg attcctttcg
     2881 gcaatccgct gctgtccgcc gactggagaa cgaatctcgg actcaccgcg cccgggacct
     2941 cggcgcaact gctgatcctg aggcgaaacg tcgagggtgg cgggcgccag gacgacatcc
     3001 ggcatacctc ctatcgcgag gtgatcggag tgaagggcac cctcccgggg aactgggatt
     3061 tcgatctctt ccagcaggtg ggccgcgtgg tctaccaggc gacatacaag aacgactttt
     3121 cgatctcccg cggcctgcgc gcgctcgacg tcgtcgtgga tcccgccacc ggcgcaccgg
     3181 tctgcgcttc cgtgctcaac ggcagcgacc cgaattgcgt gccttacaac atctggagtc
     3241 tcgggcgcgt aacgccggag gcgctggcct acctgcaaac accgggcttc cagaagggct
     3301 tcacgtcgca acaggtggcc ggcgcgaccc tgacggggga tctcggccgt tacgggatcc
     3361 ggttcccggg ctccaggacc ggcgtggaaa ccgcattcgg cgcggagcgg cgcaccgaaa
     3421 aactcgaact ggaaaccgat tccgccttct cgacgggtga cctggcgggc caggggggat
     3481 cgataatcgg cgtcgcgggc gcctacacgg tgaaagattt cttcggggag atccgcgcgc
     3541 cgctcaccgg ggcgctcagc atgagcgcga gctaccggta ttcggactac tccaccggca
     3601 agacgaccca tacctacggg ctgggtttcg atttcgcgcc ggtgaaccgg gttcgcctgc
     3661 gcggcacgta ccagcaggcc gttcgcgcgc cggacatcgt cgcgcttttt gcgccgcaag
     3721 ccgtgggaag cgagcaaatc ggggatgact gcgcaggccc ggcaccttcc cggtccctcg
     3781 ccgaatgcca gcggaccggc gtgacgccag cccaatatgg tcacatcctc gagagcccga
     3841 gcggctttta ccccgttgtt ttcggcggca atgcggacct caaaccggaa acggcgaaaa
     3901 cctggacgct gggcgtggtc ttcacgccga ccgcgcacct gaaggcgaca atcgattatt
     3961 tcagcatcgc cttgagcgac acgatcctgc ggtttttccc gactatcagc ttgagccaat
     4021 gcttggatac gggcgacccc aggttctgca gcctgatcac gcgggatcct ttacggggca
     4081 ctttgtggct gcctcccgcg cgcatcgtcg ccaccgacca gaacatcggc aggggggcga
     4141 cctcgggcat ggacgtctcg ctcagctaca cgcaccggct cgacggcaat gggaccgtcg
     4201 atatcggggc tctggggtcc tacctcagat cgttggagct cgagccttat gcgggcgcca
     4261 gtgccttcga ctgcgccggc ctgtacgggg gaggctgcgg tcaccccagc ccggtctggc
     4321 gccatcggct tcgcgcgacc tggaatacgc cctgggattt caacatcgcc gccacctggc
     4381 gctacaccag ctcgctccgg catgccggga cgagctcgaa cccgctgctc gcctcggaca
     4441 ttcaggaggt catcaggaag ctgaaggccg tgaactactt cgacgtcgcg ggttcctggt
     4501 cgctcaacag gcacctgacc ctgcgcgcag gggtcaacaa tcttctcgac cgcgacccgc
     4561 cggtggcgtt gaccgaggcg agcacctttg cacagtggta cgactccctg gggcgctacc
     4621 tgttcgtgag cctcacggcg cggttctgaa cgttcccgcg gcgccagagg caaaaaaggg
     4681 gtcaggttac ctttctgtcg cattgccgtc gcgagacggc gggctgaaaa gtaacctgac
     4741 cccttttttt gccttgctca ttttttgtgc aaagtaacct gacccctttg ttcctacatc
     4801 tgcctgaaga gatgggagta gatgcgggag acggtgaggg tctcgtcctt gcccctgatc
     4861 ttcacgtgcg cctgcccgcg gaagtcgcgc gtgacgccgg cgatggcgtt cacgttcacc
     4921 agggtggagc ggtggatctg ccagaagacc tcggaatcga gtccatccag cagctcgcgg
     4981 atcggtgtct tgatgagggc ctcggcctcg gccgtgacca cgcgcgtgta cttctcgtcg
     5041 gactggaaga agagcacatc gctcaccggg atgaggcgca ggttctgccc gaagctcgcc
     5101 ttgatccact gcagcttctc cctggccgcg ccgccgcgca attgtgttgc gagctgcgag
     5161 acgatcgcgt tcaggtccgg cggcgggctc gcgagcttgc ccttgagccg ctccaccgag
     5221 accgcgagcc gctcgtcggt atagggcttg aggatgtagt cgactgcgcc cgtttcgaag
     5281 gcctggatgg cgtactggtc gtaggcggtg acgaacacca cgtgcgcgcg tgtgcccacg
     5341 tgccgcgcca cctccacgcc cgtcatctcc ggcatctgga tgtcgaggaa catgaagtcg
     5401 ggcgcgcgct cctcgagggc ctccagcgcc gcggcaccgt cctccacgga ggcgatgatc
     5461 tccagttccg gccacagctt gttcagcttg gactcgagct gggtgcgcag gatcggttcg
     5521 tcctcggcga ggatggcggt cggcatgcgc gaatcctact gtttgagggc ccgcttgagg
     5581 atcttgccgg tcgcggtcat cggcagcgac tccacgatct gcacgatgcg cgggtacttg
     5641 taggccgcca tccgctcctt gcaccaggcc acgatcgcct ccggcgtcgc gtccttgcca
     5701 gcccgcagca ccaccacggc cttcacttcc tcgccgtgcg tctcgtgcgg cacgccgatc
     5761 accgcgacct gcgcgatcgc ctcgtgggtc atcatcactt cctcgatctc gcgcggatag
     5821 acgttgaagc cgccccggat gatcatgtcc ttcaggcggt cgacgatgta gaagttgccc
     5881 tccgggtcga cccgcccgat gtcgccggtg tggaaccatc cgtcgcggat cgcctgcgcc
     5941 gtggcttccg gccgccggaa gtagcccttc atcacgttgt ggccgcggat gacgatctcg
     6001 cccggctgtc cggtcggcac gggcgccccg gactcgtcca cgacccggac ctgcgtcccc
     6061 cagacgggct tgccgacgct gccgggaacg cgcggaaaat cgaggaagct gaacgtggcc
     6121 accgggctcg tctccgaaag cccgtagccc tcgaggatcg cgatgccgaa gcgttcctcg
     6181 aaccggcgca tcacctccac cggcatggca gcaccccccg acacgcccac gcggaggttg
     6241 gccgcgatcg acttcaggtc gtggcgctcc tcggcgccgg caaggttcag gatcgcctgg
     6301 tacatcgtcg gcacgccggc gaagatggtg accttgtgct tctgcatcgc ggcaagcgcc
     6361 gcgtcggggt cgaagcgcgg cagcaggacc atcgagcagc cgcgccagat tcccgcgttc
     6421 atctgcacgc tttgcccgaa ggagtggaag agcggcagcg cgacgaggtg gcagtcggcg
     6481 gcgaccattt gcgtgatcat gccggagacg aacgcattca tggccatgtt ggcgtgcgtg
     6541 agctccgcgc ccttcggctt tccggtcgtg cccgacgtgt agaggatgac ggcggcatcg
     6601 ttcgcgtccg tatcggcact ggtccccgtg tcgggctgcg cctgcatgaa ggcgccgagc
     6661 gtccggaccc cgtcgatcgc gcaaggcttc gcggggtcgg cggtgatcat gaagaagtgc
     6721 gggcaggcgc cgcactgcgt gaagcccgcg tgcccttcct tcccgagcgg cagttcggcg
     6781 ctgccttcga agcagaagaa agccttcgcc cgcgaatcct cgaggtggta ggcgatctcg
     6841 ggcgccttgc tgaggacgct caccggcacc acggtcgcgc cggccttgag gatgccgtaa
     6901 tagacgatcg ggaaatacgg cacgttgggg cacgcgaggg ccacgttgtc gcctcgcccg
     6961 atgcccagcg cgcgcagccc gttcgcgacc cggttggcgg cagcgtccag ttggccgtag
     7021 gtgaacaccc ggtccgcgaa gaagaccgcc ggctttcccg gatggagctg tgcgccccgc
     7081 tcgaggccca tggccaggtt gatcatcgat gtctccgcgt tcagagtgac cgggaaatga
     7141 gttccttcat gatctcgttc gtgccgccgt agatgcgcga cgcccgcgcg tcggcccacg
     7201 cccgcgcgac cggatactcc cacatgtagc cgttgccgcc gtgcagctgc aggcaggcat
     7261 cgatcagcct gcactgcagg tcggtgcacc agtacttggc catcgaggcg gtcgcggcgt
     7321 ccagcgtgcc cgccagcacc agctcgatgc accggtcgac gaacacgcgg gcgacctgca
     7381 actccgtctt ggactcggca agggagaagc gcgtgttctg gaactccagg atcgtcttgc
     7441 cgaacgcctt gcggtcccgg cagtaggcga gtgtcccgtc gagcactccc tcggccatgg
     7501 cgatcgcgga gatcgcgatc tgcaggcgct cccacggcag ttcctgcatc aggtaggcga
     7561 agcccttgcc ctcctcgccg aggaggttaa ccgcgggcac gcggacatcg gcgaagaaaa
     7621 gctcgcccgt gtcctgcgac ttgagcccga ccttggcgag ccgccggccc ttggtgaagc
     7681 cgggcatcga ggtatcgacc acgaagaggc tcgtgccgcc ggcgccctgc gacgggtcgg
     7741 tcttggccgc cacgatcacc aggtcgcagt gcgagccgtt cgtgatgaat gtcttcgcac
     7801 cgttgagcac gtagtgatcg ccgtcgcgca cggcgctcgt ccggatgcgc tgcaggtcgg
     7861 aaccggcgtc cggctcggtc atcgcgatgg ccccgatcat ctcgccggtg gccattttcg
     7921 gcaggtactg gcgcttgagc gcctcggcgc cgtagtggag gatgtagggc gccacgatgt
     7981 ccgaatgcag cgaccacccg agcaggctga tcgcccccat tcgcgcgggc tcctcgaaga
     8041 cgacgacgct gaagcgccgg tccgcgcccg cgccgccgta ttcctccggc atcgtcgggc
     8101 agaggaaccc ggccgcgcct cccttgcgcc agatctcgcg gtcgatgaag ccctgttcct
     8161 cgtagcgccc gagattcggc agcacctcga tctcgacgaa acggcgcagg gcgtcgcgga
     8221 actgctcgtg ctcgggctcg aacagcgtgc gtggaatcgc gaatggcatc ggagcttctt
     8281 gcggaagacc cggggcgtcc cgggccgatg cggcattcta tacggcaggc ggctccttcc
     8341 ccggatccgc ggcctgcgtc gcctgcgcct tcatcaccgg ctccaccttc ggcgccgcaa
     8401 ccggctgcgg gtccttcttc ttgcgcagga tccaccagat cagcaggacc accaggacca
     8461 ccggcgccag cgcgggcgag agggcgatga ggatcacgac ggggatgaag atcgccagcc
     8521 cggcaaggag ccaccccagg ccgtagatga gcgccgccac gatggccagc acgagcacca
     8581 cggcggcgaa gccgatcgcg atgcccgcgg cccccagaag cgcgccgctc gtgcccgtga
     8641 gcgtctcggt gccgacctgc accggaacca atcccgtcag cacaccgaaa acgccgaggc
     8701 ccgagacgac cgcggccacc accaccaggg ccacgaaggc gaacgaaagg gtcttcctcc
     8761 aggtgcgctc ggccgttccc acggcggaca gcaccttgcc tgcggccgac ttcgccgcgg
     8821 caggctgcgc ctccccgcgg gagccggggt cgttgcccgg ccacgcgaag ctgttggtcg
     8881 cggcagcgcc cggaggcacg gcggtggccg cgcgcaaccc gttaagcgga acctcgatcg
     8941 tcgcgatgac gccgctgggc gaattcgatt ccagcgtgag cctggccttg tcgccgtaaa
     9001 ggccggccag ccgctcgcgg atgttggcca gcccgacgcc cgtgcccacc gtttcgccga
     9061 agccgcggcc ggtgtcggat accacgagcc gcagctgccc gtcctgcgcc tgcgcggtga
     9121 tgagcacgct tccgccctcg cgcagcggtt ccagcccgtg cttgatggcg ttctcgacca
     9181 gcgagggcac catcaacggc gggaacggcg tggcgagcag gctctccggc accgcaatct
     9241 cgaacgaaag ccgcttgccc atgcgcatct gcagaatgtt gagataggcg cgcaccagct
     9301 cgatctcctg cccaaccgac gaaaccgact cccgcatctt gggcagcgcg ctcctcaggt
     9361 actggatgag gtggcccacc atcgtgttgg cctgcgccgg atcgacctcg gtcagggcct
     9421 gcacgttggc gagcgtgttg tagaggaagt gcggctcgac ctgcgcctgg agcgcctgca
     9481 gcttcgcctc ggtcacctgc tggctcatgc ggtggtactc ggcttccttg cgcttcaggt
     9541 cggccatgcg ctgtgcggca cgcgaacggt cgatgaagaa cttggccacc acggtgagga
     9601 tgaagagcgg gatgaagagc agcaccaggc cggcgccgat gccgatcttc ctcaggtcgt
     9661 cgcctacgtt gcgccggatc tccgcgcgaa tttcgggcgg cagcggcggc agggcgacgg
     9721 tcggcggcgt tggcggcgtc ggggtcatgc cctcgggcat gttgccgagg tcgagggtga
     9781 tggatccgcg cggccccttg accgtggcgc cgggcgcatg gatttcgacg tgcggccttt
     9841 cgccgggatc agccttcgag acgtcgatat ccaggagcgg gcggtccttt cccgcgccca
     9901 tgccgatcac gatccgcttg cgcgtctcgg cgcctttcgc gtcggtctcc agggccttct
     9961 tcgcctcgtc ctcggcctcc ctggccgact tgagggactg ctcgagggcc gccttcacct
    10021 tgtcgttctc gaggcccgcc gccttgagcg cctgctcggc gttgcgccgg gcctcctcga
    10081 tggcggaggc ggcctcgcgg gtggcctcgc gcgcggtgtc gatcgcttcg cgcttggccc
    10141 tgagcacctc gcggccggcg tccctcaatt cggcgcgggc gccgtccagc tcctcgatgg
    10201 cgcggtcgag ttccgcgcgg cgcagcggat cggacgtggc gccgcgcaac gcgaggatcg
    10261 cgcttctcgc cacgtccacg gccccttccg cgaaggtatt gtcgatctcg cgcttgatgg
    10321 cccggttttc ctcgggcgtg gcgccttcct tgcggtactc gatcaggtgg aacggggtca
    10381 cgaagacggc cagcgccacg atgatggcaa agccgccgat gacgagcgcc caccagggcg
    10441 tgcggtggat cgagtcggaa aagggtttca tgggatcggg tttcgcgggg cccccgggaa
    10501 gatacccgga aggagcccgc cggtcagtag tcttcgagaa tcgccaccgc ggcgaggacg
    10561 accgagagca tcagcacgcc gaaaaacagg aaaaacgtcg atgccgacgg cccgagcgac
    10621 tggatgaagg acatggttcc ggctccctgt tggcttggcg gccccggggg gcctcgatgg
    10681 aaccagatta ggggcggcgc agcggcgggg cgacgttcaa gcgccggatt gcaggaaacg
    10741 gggggcgaag tgcaggcagg cgggggcggc cggcaggagc ggtgtcagac acctcacatc
    10801 tgcttgaaca ggtgggtgaa gttgcgcgag acgataaggg tctcgcgatg ccccttgagg
    10861 gtgatctcgg cctggtcctt gaggccgcgg cgcacggccg cgatggcttc cacgttgacg
    10921 atggtggcgc ggtggatctg ccagaacttc tcggcgtcga gctcctcgag gagctccttg
    10981 atgggcttgc ggatgagggt ctcggagtcc gccgtcacca cgcgggtgta cttctcgtcg
    11041 gactggaaga agagcacttc ctcgaccggg atgagccgca cgctctgccc gacgcttgcc
    11101 ttgatccagc gcaggctctc ggcggcacgg gggaccagcc ggccggccag ccgctcgacc
    11161 agctcttcca tcgccggggg cgcgtccgaa agccgctcgc ggagtcgctc gaccgtgctg
    11221 gcgaggcgct cctcgttgaa gggcttgagt acgtagtcga tggccccccg ctcgaaggcc
    11281 tcgatcgcat actggtcgaa cgccgtgacg aacaccacgt gcgcccgctt gccgatggtc
    11341 cgcgccgcct ccagcccgtt gacaccgggc atgtggatgt cgaggaagaa gagacgcggc
    11401 gcgtgtgccg cgatcacctg ctcgatgtcg cggccgttct ccatctcatg gacgatcgcc
    11461 agctcgggcc agagcttgcc caggcgcgag cgaagctgcg cacgcagcag cggctcgtcg
    11521 tccgcgatga cggcggtagg tatccccatg aaacgggagt atcagacaca cggcgccgca
    11581 acggaagatg caggcaacgg cacctcgatg gcggagaagg cgtccacttc gatggttgcg
    11641 atcaccccgt gcggcgtgtt ttcctccatg aggagacggg ctcccgatcc gtgcagggcc
    11701 ttcaggcgtt cgcggatgtt gacgagcccg acccccgttc ccttcctcgg cgagatgcct
    11761 tccccggtgt cggccacgga aaagcgcagc ttcccgccct cctcacgggc cctgatcgtg
    11821 atcgtgccgg cctcgcagca gggatcgacc ccgtgcttga tggcgttctc gacgagcgag
    11881 atcagcatca tgggggggaa cggccggcag cgaagcgcct ccggcacgtc gaggacgtag
    11941 tccaggcgcg agcccatgcg cattcggtgg atatcgagga acgcccgggc catgtccgcc
    12001 tcgcggccga cggtggttgc gtcctcgcgc atgcggggca acgctgcgcg caggtactgg
    12061 atgaggcttt ccagcatccg cgaggccgag gccggatcgg tctcgacgag atgctggacg
    12121 ttggcaagcg tgttgaagag gaagtgcggc tccacctgcg cctgcatcag cgccagttgc
    12181 gcctggagca cgttcttctc cagctgcgcg cgttcggcct gcgcgcgggc aagctcggcc
    12241 gcgtggcgcg attcccgctc ccggaatacg tacacccaca ccgccatggc cccgaagccg
    12301 atgcccagcc ccatggtgat gagcaccccg tagaagcgct cgctgaacat ggtcgcgaaa
    12361 tcgcgcccct tcacgatgcc ggcgagcatc gtcccgagga aggagcccac gacgatggac
    12421 agcacctgca ccgcccagcg cggcagcttc cgctggcgca ggttcgtggc gacggtgaag
    12481 aggaccatgc tggtgtagcc cacgcacagg gagctcacca ggagatcgag gtaggtgttg
    12541 acgaagaacc agaccgtcgc cgccgccgcc cagacgcaca ggaacgaaac cagcagcacg
    12601 cgcctgagcg tgagaccctc gaaaagatag gcgtagcggt tcacggcggc cggtggggaa
    12661 atgtagtcgc cggaagcgta tcagttgccg gcggcgctga cgcggtcgcg cggcttccag
    12721 cgaggagcga tgcccgggcg gggggaggac cagggaagcg ccgccgaccg ttgctggggc
    12781 tggcgcatgg cgtccccgga ccgcaggcgg atggtccacg gcagggtcat caggttggca
    12841 ggtcggctca tggcatttct ttcagttacc gaaggttacg cggggcaccg tggccgaacc
    12901 acgagcctgc gacgaaacgc gccgccgggt gacccggagg cgccgaacgg cgacaagcgg
    12961 gacaggtaat ggggacggtt ctctgtaccc ggtacatgta ccgggtacag agaaccgtcc
    13021 ccaaaaaaaa cccggccgca gccgggtgga gcacaggacc aacaggcatt caggcaccga
    13081 cgacgagccc cccgacggtg atggcgaagg tgagcgcgaa ccaacccgcc acgatcgccg
    13141 tgattgcggt agagacgccg agggcgtgcg acgaaatgat ccgttcgatg agtttcatgg
    13201 cgatctcctc aggggcggaa ggcgaacggc agcgcgactt ccagcgacgg gccgccagcc
    13261 ggccggaccg aggccgtgcg cgtcggcgcc gcattcgggg tgcccgcatc gtcacgtgcg
    13321 gccgtcacga caagccgcat gcgaccgtcg tcggtgaggg ttacggcctg ctcctgggcg
    13381 gcatgccggg cctggggcgc ggcggaatgg tacgaggtca ccatccagcc cacgagaagc
    13441 gcggcagtga cgacggccag cgaggcgacg cccatcaggt ccagcttctc gagtttgctc
    13501 atggtgaccc tccttcacgg ctcggcgccc ggcgggcgcg ttcgacttga cgccagatta
    13561 aggaaggaca aagaggggct gaagcggaaa gcgacgaaat gcggaaaagg aggcgcgaaa
    13621 cgccacccag gcgccaaatg cggccttgct tcaggctaga ccgcggcctc gcgctgcagg
    13681 ctgcgcatgg cgagcgagcc gacgatcatg ccgccgaagc tcgcgacgag gccggcgagc
    13741 tgcgggggca tggtcccgtc cgcgccgtag cgctcgaagc cgatccaggt tacgaggccg
    13801 aaaatgatgg cgagcgtggc gcccaggttg gtggcgcgct tccagtagag cccgcacacg
    13861 agcggcacga aggcgcccac cagcgtgacc ttgtaggcgt tctcgaccat cttgtagatg
    13921 ctcgcctcgg tgctcagggc gacggccgtc accacgaccg tgaatcccac gatcaccacg
    13981 cgcatcaagt gcaggaatcg ctggtcgccg atgtgcggca ggagcggccg caggatgttc
    14041 tcgctgaagc tcaccgaggg ggccagcagc gtcgccgatg cgcagctctt gatcgccgag
    14101 agaagcgcgc cgaagaacat gatctgcgcg acgatgggca ccaccggcga aaggatcagg
    14161 ttcggcagga tgagctgcga gtcgcccgcg aggttggcct ccaccatctt cgggtcgatg
    14221 agcgtcgcgg aataggcgag gaacatgggg atgaacgcga agccgaaata gaggacaccg
    14281 cccaggatcg acgagcgcgc ggcgatgttc tcgttcctgg cggactgcac gcgctggaac
    14341 acgtcctgct gggggatgga gcccagcatc atcgtgatcc aggccgccag gaaccagagg
    14401 atgtccttga gctccggcgc cggcaggatg tggaacttgt cgttggcggc cgcgtgcgcg
    14461 atcaccgtgc cgacgccgcc ggccagcccg ctcacctccc agccgatata gagcatgccg
    14521 agcacgatga tgatcatctg gatgaaatcg gtgatcgcca ccgagaacat gccgcccagc
    14581 atcgtgtaga ccagcaccgt cgaggcaccg atgatcatgc cccactgctg cgtgatggcg
    14641 ccttcggaga cgacgttgaa gaccagcccc agcgccttga tctgcgcgcc tacccagccg
    14701 aggtacgaaa tgacgatgca aagcgtggtg agcatctcca ccggccggct gtagcgcttg
    14761 cggtagtagt cgccgatggt gagcaggttc atgcggtaga gcggccgcgc gaagaacagg
    14821 cccaccagga cgaggcacag cgacgagccg aacggatcgg cgatcacgcc ggagaaccct
    14881 tccttgagga aggtggcggg gatgcccagc accgtctcgg agccgaacca ggtcgcgaac
    14941 accgtcgcgg tgaccacggc catcggcatc gcgcgcccgg ccacagcgaa atccttcgag
    15001 ttgtggacga acttggcggc gtagatgccg atcgcgacgg agatgatcca gtagatgatg
    15061 acgaaccaga ggagcatggg cgggtcagcc gtcagcgcgc gcggaagaaa aaggatgtcc
    15121 gggaaatcgg gccgagtgta gcaaaatcaa tgctgtagcg cagtaccggg tacatgtacc
    15181 cggtacacaa atggcggcac ctacttgatg cccgcctgca tgaacgattg catgaactgg
    15241 cgctggaaga ggaggaacgc gacgagcagc ggcgagaccg ccatcagggt gccggcgctg
    15301 atcaccgccc agtcgacgcc ggattccggg gcgccgaaaa tcgcgagccc gaccgtcagc
    15361 gggcgcgtgt tcacggagtt cgtgatgatc agcggccaga ggaagttgtt ccagtggtag
    15421 ctcaccgaca ccaggccgta ggcgaggtag gtgggcttgg ccagcggcac gtacacccgc
    15481 agcagcacgc cgatgaagtt gcagccctcc acgc
//
LOCUS       DASUEV010000335         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_106108, whole genome shotgun sequence.
ACCESSION   DASUEV010000335 DASUEV010000000
VERSION     DASUEV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106108"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..2123
                     /locus_tag="VFV55_10660"
     CDS             132..2123
                     /locus_tag="VFV55_10660"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEX4944806.1"
                     /translation="MEAKHLEAVYRTMLRIRRFDEGVMNLFKEGLVKGTAHSYVGQEA
                     VAAGACAALTKADFVVSHHRGHGHCIAKGASLDRMMAELMGRVTGYCKGLGGSMHIAD
                     LEHNILGANGIVGAGIGIGTGAALTNKLRGNDAVGVVFFGDGAANEGIFHEGLNLGSL
                     WKLPVVYLCENNQYGLSTSMKDSTAVDRISKRAGAYSMPGVTVDGNDVLAVHAAVQEA
                     VARARKGDGPTLIEALTYRWGDHSMRANLPRYRTEAEEVEWHKLDAVARFEQKLETTY
                     GFKRAALDAIGAEVEAELGVAKAFAIGSPEPTLDDLEGAVYAPHYRPQEPRLAAPREL
                     SFTEAIKEALAQEMERDPRVFVLGEDVGKIGGIFAATRGLIEKFGPERVRDTPISEAA
                     IAACAVGAAITGMRPVAEVQIFDFITHMMDMIVNQAAKFRFMLGGKPTVPLVIRGPQG
                     GGIRLAAQHSQSLEAWFTHVPGLVVLGPSSAYEAKGLLISAIREDNPVIFLEHKMLYV
                     GKKAPVPEEAYAIPIGKAVIKRHGSDVTLVATMAMVESALQAATRLEADGIDVEVIDP
                     RTLRPLDEDTIIQSVKKTSRLVIAHEGWKRSGFGAEVAAMVAEKAIDWLDAPIVRVGA
                     RESPMPYNDKLERLVIPQAEDIVDAIKAVVLRDRVGAKA"
BASE COUNT          471 a          754 c          809 g          391 t
ORIGIN      
        1 acgagcagaa tctggcggca taatgccgtt ggcttggaag acgaaagaca gagggaagct
       61 cacgcctgca ctccctcatc aaaccgtgca taaggcggga cgcgacggag tcgacaaacg
      121 aggggggcca catggaagcg aagcacctcg aggcagtgta tcgaacgatg ctgcggattc
      181 gccggttcga cgagggcgta atgaacctgt tcaaggaggg cctcgtgaag ggcacggccc
      241 acagttacgt cggacaggag gcggtggccg cgggagcttg cgccgcgctg accaaagcgg
      301 acttcgtcgt gagccatcac cggggtcacg gacactgcat agccaaaggc gcctccctcg
      361 accggatgat ggctgagctg atgggacggg tgacgggcta ctgcaagggc ctgggcggct
      421 cgatgcacat cgccgatctc gagcacaaca tcctcggggc taacggcatc gtcggcgcgg
      481 ggatcggaat cggcaccggg gctgcactga cgaacaagtt gcgcggcaac gacgcggtcg
      541 gcgtcgtgtt cttcggcgac ggggccgcga acgagggcat ctttcatgaa ggtctcaacc
      601 tcggctcgtt gtggaagctg ccggtggtgt atctctgcga gaacaaccag tacggtctgt
      661 ccacttccat gaaggactcg acagcggtcg atcgcatctc caagcgagcc ggcgcgtatt
      721 cgatgcccgg ggtcaccgtc gacggaaacg acgtgctggc cgttcacgcc gccgtacagg
      781 aagcggtggc cagagcacgc aagggtgacg gaccgaccct gatcgaggcg ctcacctatc
      841 gctggggcga ccattcgatg cgcgcaaacc tgcctcgcta ccgtaccgaa gccgaagagg
      901 tggagtggca caagctcgac gccgtcgccc gcttcgagca gaaactcgag accacatacg
      961 gcttcaagcg cgccgcgctg gatgccattg gcgcggaagt cgaagccgag ctcggcgtgg
     1021 ccaaggcgtt cgccatcggc agtcccgagc cgaccctgga tgacctcgag ggcgccgtgt
     1081 acgccccgca ctaccggccg caggagccca ggctcgctgc cccgcgtgag ctgtccttca
     1141 ccgaagccat caaggaagcg ctcgcgcagg agatggagcg cgacccgcgc gtgttcgtgc
     1201 tgggcgagga tgtgggcaag atcggcggta tcttcgccgc cacccgcggg ctcatcgaga
     1261 agttcggtcc ggaacgggtt cgcgacacac cgatttccga ggctgccatc gcggcctgtg
     1321 ccgtgggcgc ggccatcacc ggcatgcggc cggtcgccga ggtccagatc ttcgacttca
     1381 tcacccacat gatggacatg atcgtcaatc aagcggccaa gttccgcttc atgctgggtg
     1441 gcaagccgac cgtgccgctg gtgatccgcg gcccgcaagg cggcggcata cggctcgccg
     1501 cgcagcactc gcaaagcctg gaggcgtggt tcacacacgt gccgggtctg gtcgtactcg
     1561 ggccctcctc agcctatgag gcgaagggac tgctgatcag cgccatacgt gaagacaatc
     1621 cggtgatctt cctcgaacac aagatgctgt acgtgggcaa gaaggcgccc gtgcccgagg
     1681 aggcctacgc gatccccatc ggcaaggcgg tgatcaagcg tcacggaagc gatgtgacgc
     1741 tggtagccac aatggcgatg gtggaatccg ctctgcaggc ggccacgcgg ctggaggccg
     1801 atggcatcga cgtcgaggtg atcgatccgc gcacgttgcg gccgctggac gaggacacga
     1861 tcatccaatc ggtcaagaaa acgagccggc tggtgatcgc ccacgaaggc tggaagcgct
     1921 cgggcttcgg cgccgaggtc gccgccatgg tcgccgagaa ggccattgac tggctcgatg
     1981 cgccgattgt gcgcgtgggc gcgcgcgagt cgcccatgcc atacaacgac aagctcgaac
     2041 ggctggtcat cccgcaggca gaggatatcg tcgacgcaat caaggccgtc gtgctgcggg
     2101 atcgggtcgg cgcgaaagcc tagcccgaat ttctgccggg gcccgttccg cgcgcgcaac
     2161 agtcgggggc aagccccggg acgggactgc aaggtcctac gcgacggggt cagtacacga
     2221 cggggtcttg ttccctacaa gggtttttac aaaccttccc cattgcccgc acgcttcgca
     2281 tcgagcaccg cggcgctttg tatcagccca gcactggccc accccgtcga tcacgcctcg
     2341 atatacataa tcatttcgag ttcccggaag cggtctttaa tctgcgtcga ctgcattgga
     2401 gctgtgcccg cccgaaggcg gtggg
//
LOCUS       DASUEV010000336         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1018568, whole genome shotgun sequence.
ACCESSION   DASUEV010000336 DASUEV010000000
VERSION     DASUEV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018568"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFV55_10665"
     CDS             <1..223
                     /locus_tag="VFV55_10665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX4944807.1"
                     /translation="AKLLEAIARPFDGRAGRMCVSASIGGSFLPGPEPDADALVRAAD
                     GALYESKREGRNRYTEAPATEVPRAVAAA"
     gene            complement(220..810)
                     /locus_tag="VFV55_10670"
     CDS             complement(220..810)
                     /locus_tag="VFV55_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX4944808.1"
                     /translation="MATVYATLAQGAVPASAAKPDLERGKQIAATVCAACHGPDGNSL
                     VPANPIIAGQHADYLAAQLAAFKSGARANPIMAGMSAALTPEDMRNVSAWFSQQVVKP
                     SAAKDQALAIRGQQIWRGGIKQGNVPACAGCHGAAGAGIPAQYPRLAGQFADLTFTWL
                     KAFASGARANPVMAGIAAKLSEADMKAVSEYVAGLR"
     gene            953..1573
                     /gene="yihA"
                     /locus_tag="VFV55_10675"
     CDS             953..1573
                     /gene="yihA"
                     /locus_tag="VFV55_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX4944809.1"
                     /translation="MRLENARYHLSVHDAHLLPGDSLPEVAFLGRSNAGKSSALNTLA
                     GRRRLAFVSKTPGRTQLINYFAVDEDAFLVDLPGYGYAGVPYEVRQHWDRLVGEYVFH
                     RRALAGVIIVMDSRHPLAAQDRRLLDWLLPSGRNVHVLLSKSDKLSAQAAHRTLVQAR
                     KDLAQVYPGATAQLFSALKRTGMAEAQARIESWVEEHKQKAPAKGD"
     gene            1708..2715
                     /gene="hemB"
                     /locus_tag="VFV55_10680"
     CDS             1708..2715
                     /gene="hemB"
                     /locus_tag="VFV55_10680"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX4944810.1"
                     /translation="MTFPYSRFPQRRMRRMRRDDFSRRLARESRLSPDDLILPVFVLG
                     GAHSIQPVASMPGVNRRSVDELLPIVEQALTLGIPAITLFPVVDASAKSLGAEEAYNP
                     DGLVPRCIREVKARFPEIGIITDIALDPYTSHGQDGLIDGSGYVLNDETIAVLEKQAL
                     AHAEAGADVVAPSDMMDGRVGALRRALDTGGHIHTRILAYSAKYASAFYGPFRDAVGS
                     GANLGKGDKKTYQMDPGNSDEALHEVALDLDEGADMVMVKPGMPYMDIIRRVKDTFGV
                     PTFVYQVSGEYAMLQAAAAQGWIDGPSCMMEALLGFKRAGADGILTYFALDAAERLRA
                     R"
     gene            complement(2712..>3130)
                     /locus_tag="VFV55_10685"
     CDS             complement(2712..>3130)
                     /locus_tag="VFV55_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944811.1"
                     /translation="YQGLYQSDNQPAELRHLREVAVKVMREFADFGPMLVGAVLNGTA
                     NQFSEVTLQLFADDAKSLALFLVNRRHRFEQADKRVRIGDDWVDVPQFFLEVDGAPVT
                     LSVYARGDEHLAPRARPEADGPQRARLAEVEALLGP"
BASE COUNT          529 a         1057 c         1045 g          499 t
ORIGIN      
        1 cgcaaagctt cttgaagcca tcgcgcggcc gttcgacgga cgcgcgggac gcatgtgcgt
       61 gagcgcgagc atcgggggaa gctttcttcc cggcccggaa ccggacgcgg atgccctggt
      121 ccgcgcagcc gacggagcgc tctacgagtc gaaacgggaa gggcgcaacc gctacaccga
      181 ggcgccggcg acggaagtcc ccagagccgt tgcggcagcc tagcgaagac cggcgacgta
      241 ctccgagacc gccttcatgt cggcctcgga cagcttcgcc gctatgccgg ccatcacggg
      301 gttggcccgg gcaccgcttg caaaggcctt gagccaggtg aaggtgagat cggcgaactg
      361 gccggcgagc cgggggtact gcgcgggaat tccggcaccc gcggcaccgt ggcagccggc
      421 gcaggccgga acgttcccct gcttgatgcc gccgcgccag atctgctggc cgcgaatcgc
      481 aagggcctgg tccttcgccg cggaaggctt caccacctgt tgcgaaaacc acgcggagac
      541 gttgcgcatg tcctcgggcg tgagcgccgc cgacatgccc gccatgatgg gattggcgcg
      601 cgcgcccgac ttgaaggccg cgagctgcgc cgcgaggtaa tcggcgtgct ggccggcgat
      661 gatgggattg gccggtacga ggctgttgcc gtccgggccg tggcatgccg cgcacacggt
      721 ggcggcgatc tgcttgccgc gctcgaggtc gggcttggcc gccgatgccg gaacagcgcc
      781 ctgcgcaagc gttgcgtaaa ctgtcgccat gagccctgcc caccgaaccg atgcgtgcat
      841 tgccgatcct ctgtctgtcg agtcgcgtaa accggatgat tttaccgtag tgccccctca
      901 cgccgccacc ccggtgccgc cggcggcgag gccgggctga ggcgggccgc gaatgcgcct
      961 cgagaacgca cgctaccacc tgtcggttca cgacgcgcac ctgcttcccg gcgacagcct
     1021 gccggaggtg gccttcctgg gccgatcgaa tgccggcaag tcgagcgcgt tgaacacgct
     1081 cgcgggccgg cgccggcttg ccttcgtgag caagacgccg ggacgcaccc agctcatcaa
     1141 ttacttcgcg gtcgacgagg atgcgttcct cgtcgacctg ccaggctacg ggtacgcggg
     1201 cgtcccgtac gaagtgcgcc agcactggga caggctggtc ggggaatacg tcttccatcg
     1261 gcgcgcgctc gccggcgtga tcatcgtgat ggattcgcgg caccccctcg ccgcccagga
     1321 ccgtcgcctt ctcgactggt tgcttccctc ggggcgaaac gtgcacgtgc tcctgtcgaa
     1381 atcggacaag ctgtcggcgc aggccgcgca ccgtaccctc gtccaggccc gcaaggatct
     1441 tgcgcaggtc tatccgggag ccacggcaca gctcttctct gccctgaagc gcaccggcat
     1501 ggccgaggcg caggcgcgca tcgagtcgtg ggtcgaggag cacaaacaaa aagccccggc
     1561 aaaaggggat taagaccggg gcaaaaaaag ccttaatggg aattaaggca cccgctcagg
     1621 gaggagaagc ggggaagggg tcacccttca tgcgatcaga gtcggccgcc atgctaaagt
     1681 tcccgcgtca tccacgaccg attcgcaatg acctttccat actcacgttt tccgcagcgc
     1741 cgcatgcggc gcatgcgccg cgacgatttt tcgcgccgcc tcgcgcgcga gagccgcctg
     1801 tcgcccgacg acctcatcct gcccgtgttc gtcctcggtg gcgcccattc gatccagccg
     1861 gtggcttcca tgcccggggt gaatcgccgg tcggtggacg agcttctccc catcgtggag
     1921 caagcgctca cccttggaat tccggccatc acgctgtttc ccgtcgtcga tgcatccgcc
     1981 aagtcgctcg gcgcggagga agcgtataac ccggacggcc tggtgccccg ctgcatccgc
     2041 gaagtaaagg cccgcttccc ggagatcggg atcatcacgg acattgcgct cgatccctat
     2101 accagccatg gccaggacgg gctgatcgac gggtcaggct acgtcctcaa tgacgaaacc
     2161 atcgccgtgc tcgagaagca ggcgcttgca catgcggaag cgggcgccga cgtggtggcc
     2221 ccgtcggaca tgatggacgg gcgtgtgggg gccctgcggc gcgccctgga caccggcgga
     2281 cacatccaca cccgcatact cgcctactcg gccaagtacg cctcggcttt ctacggcccg
     2341 ttccgcgacg cggtcgggtc aggggcgaac ctcggcaagg gcgacaagaa aacctaccag
     2401 atggacccgg gcaattccga cgaggcgctg cacgaggtcg ccctcgacct cgacgagggt
     2461 gcggacatgg tcatggtgaa gccgggcatg ccctacatgg acatcatccg gcgggtcaag
     2521 gacaccttcg gcgtgcccac cttcgtctat caggtcagcg gcgagtacgc gatgctccag
     2581 gctgccgcgg cccaggggtg gatagacgga ccgtcctgca tgatggaagc cctgctcgga
     2641 ttcaagcggg cgggggcgga cgggatactg acctacttcg cgctcgacgc agccgagcgc
     2701 ctgcgggccc gctagggtcc caggagtgcc tcgacctccg ccaggcgggc gcgctggggg
     2761 ccgtcggcct ccggccttgc ccggggagcg aggtgctcgt cgccccgcgc gtacaccgac
     2821 aacgtgacgg gcgcgccatc cacctcgagg aagaattgcg gcacgtcgac ccagtcatcg
     2881 ccgatgcgca cgcgcttgtc cgcctgctcg aaacgatggc gacgattgac caggaacagc
     2941 gcgaggctct tcgcgtcgtc cgcaaacagt tgcagcgtca cctcggagaa ctggttggcc
     3001 gtgccgttga ggaccgcgcc cacgagcatc gggccgaagt cggcgaattc gcgcatgact
     3061 ttcaccgcaa cctcccggag gtggcgcagt tcggcgggct ggttatcgct ctggtagagc
     3121 ccctggtatt
//
LOCUS       DASUEV010000337         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1246902, whole genome shotgun sequence.
ACCESSION   DASUEV010000337 DASUEV010000000
VERSION     DASUEV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246902"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFV55_10690"
     CDS             complement(<1..934)
                     /locus_tag="VFV55_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944812.1"
                     /translation="MSASTVLRAATLAVALVFAATADAKTLRYSSQGDITTIDPHANN
                     EGFTNAYLDNIYEPLVTRGKDLKVEPCLAVSWQQASPTVTRFKLRPNVKFHDGTPFSA
                     DDVVFSIGRALSDTSNFKPYLAGVKEARKVDDLTVDIVTEGPAPVLIPQLTEVRIMSK
                     AWATKYNVGKPQDYRNKEETFASRNANGTGPYILRTREADVKTVAVLNSNWWGKMDGN
                     VNEIVYQPIKSDGTRLAALISGEIDFVLDPPPQDVPRLKKDPKTKVVEGNENRTVFLG
                     MDQWRDELAYSSVKGKNPFKDKRVREAFQTAIDVN"
     gene            1143..1826
                     /locus_tag="VFV55_10695"
     CDS             1143..1826
                     /locus_tag="VFV55_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944813.1"
                     /translation="MTRWIALAIAALALSACGKDEPPKPVDPASKVEQATRQMGEATR
                     QGDVHQVGEAMKQMGEALAGSVKVEPVDFRTLKELLPASIAGLKRVNAEGSRTNVVGI
                     ASSRAEATYEDGKGGRVILEIKDMGTLSGVTAMATAWVNVEIDKDGDTGYERTTTVGG
                     RKAYERYDKAKRSGELDVIVAGRFIVGARATGVEMKVFKEAVARLDLAKLEALKQQGL
                     PPAPPVPAK"
     gene            1834..2625
                     /locus_tag="VFV55_10700"
     CDS             1834..2625
                     /locus_tag="VFV55_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX4944814.1"
                     /translation="MTSPSPLVSPQGASYELRDVPAFSPFAWLVRGWDDFRAAPVASG
                     FYGAAFAAMGWALLALYDFAYQYVTTLAGAFLLAAPFLATGLYALSRDREAGRKPRLL
                     ASLTAWRENMGGLGIYVLVITVIFLVWARASLVTFALFSTGPMPDLRNFLAKAFSLGN
                     IEFVIVYFAAGAMFATLVFAVSVVSVPMMLDRGNDAVSAAILSVQVLAKNPGAMAVWA
                     FVIVLLTGIGLATAFVGLVLTMPVIGHATWHAYRDCVAWGEGDPP"
     gene            2622..>3073
                     /locus_tag="VFV55_10705"
     CDS             2622..>3073
                     /locus_tag="VFV55_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX4944815.1"
                     /translation="MTEQDRADNPMGTDGFEFIEYTAPDPKLLGALFEQLGFTLVATH
                     RSKDVALYRQGGVNFIVNREPDSFAQSFARVHGPSACAFAIRVRNAAKAWQRALELGA
                     KPHHGPVGPMELNIPAVKGIGGSLIYLVDRYPGNGGNLSIYDVDFVPV"
BASE COUNT          459 a         1004 c         1065 g          545 t
ORIGIN      
        1 cgttcacgtc gatggccgtc tggaaggcct cgcgcacgcg cttgtccttg aacgggttct
       61 tgcccttcac gctcgagtag gcgagttcgt cgcgccactg gtccatgccg aggaatacgg
      121 tgcggttctc gttgccttcc accaccttgg tcttcgggtc cttcttgagg cgcggaacgt
      181 cctgcggcgg cgggtcgagc acgaagtcga tctcgccgga gatgagcgcg gccagccgcg
      241 tgccgtcgga cttgatcggc tggtacacga tctcgttcac gttgccgtcc atcttccccc
      301 accagttgga attgaggacc gcgacggtct tcacgtccgc ctcgcgggtg cgcaggatgt
      361 aggggccggt gccgttggca ttgcgcgagg cgaaggtttc ctccttgttc ctgtagtcct
      421 ggggcttgcc gacgttgtac ttggtggccc aggccttgct catgatgcgc acttccgtga
      481 gctgcgggat gaggaccggg gccgggccct cggtgacgat gtccaccgtc aggtcgtcga
      541 ccttcctggc ttccttcacg cccgcgaggt agggcttgaa gttcgaggtg tcggagagcg
      601 cgcgtccgat cgagaacacc acgtcgtcgg cgctgaacgg cgtgccgtcg tggaacttca
      661 cgttggggcg cagcttgaag cgcgtcaccg tcggacttgc ctgctgccac gacaccgcga
      721 ggcagggctc gaccttgagg tccttgccgc gcgtgacgag cggttcgtag atgttgtcga
      781 ggtaggcgtt ggtgaagccc tcgttgttcg cgtgcgggtc gatggtggtg atgtcgccct
      841 ggctcgagta gcgaagcgtc ttggcgtccg cggtggcggc gaagacgagg gccacggcga
      901 gggtcgccgc gcggaggacg gttgaggcgg acatggcgga cttctcccgc gaaatgaacg
      961 gtttacgagc ggcgccgcga gcgcgaagcc ggcgccgctt ggcccgaaga ttcaaggact
     1021 tggcggctgt ttgccaatcg tcgcgatgat gccgcacggc cccccctgcg tcaacgcgag
     1081 agtcggtgtc cgtttgaagt atcctgcccc caattctccg acactcgacg aaaggcatcg
     1141 cgatgacccg atggatcgcc ctggcaatcg ccgcgctggc gctttccgcc tgcggaaagg
     1201 acgagccccc gaagccggtc gaccccgcgt cgaaggtgga acaggcgacc aggcagatgg
     1261 gcgaggcgac aaggcagggc gacgtccacc aggtgggcga ggcgatgaag cagatgggcg
     1321 aggcgctcgc gggttcggtg aaggtcgagc cggtggactt ccgcacgctc aaggagctgc
     1381 ttcccgcgtc gatcgcgggc cttaagcggg tgaacgcgga aggctcgcgg accaacgtcg
     1441 tgggcatcgc gtcctccagg gccgaggcaa cttacgaaga cggcaagggc gggcgcgtca
     1501 tcctcgagat caaggatatg ggcacgctct ccggggtgac cgcgatggcc acggcgtggg
     1561 tcaacgtcga gatcgacaag gatggcgaca cgggctacga gcgcaccacc accgtgggcg
     1621 gtcgcaaggc ctacgaacgc tacgacaagg cgaagcgttc gggcgagctc gacgtgatcg
     1681 tcgccgggcg cttcatcgtc ggcgccaggg ccacgggcgt cgagatgaag gtgttcaagg
     1741 aagccgtggc gaggctcgac ctcgcgaagc tcgaggccct caagcagcag ggcctgccgc
     1801 cggccccgcc cgtccctgcg aagtgaggcg ccgatgacga gtccctcgcc cctggtctcg
     1861 ccgcaaggcg cctcctacga gctgcgcgac gtgcccgcct tttcgccctt cgcgtggctg
     1921 gtgcgcggct gggacgactt ccgcgcggcg cccgtggcga gcggcttcta cggggcggcg
     1981 ttcgcggcca tggggtgggc gctcctcgcg ctgtacgact tcgcctacca gtacgtgacc
     2041 acgctggccg gagccttcct gctcgccgcc ccgtttctcg ccaccggcct ctatgcgctg
     2101 tcgcgcgacc gggaggcggg acgcaagccg cggctcctcg cctcgctcac cgcgtggcgc
     2161 gagaacatgg gcggcctcgg gatctacgtg ctcgtcatca ccgtgatctt cctcgtctgg
     2221 gcgcgcgcct cgctggtcac cttcgcgctc ttcagcaccg ggccgatgcc ggacctgagg
     2281 aatttcctcg ccaaggcgtt ctcgctgggc aacatcgagt tcgtgatcgt gtatttcgcg
     2341 gcgggagcga tgttcgcgac gctcgtcttc gcggtgagcg tggtgtcggt gccgatgatg
     2401 ctcgaccgcg gcaatgacgc ggtgagcgcc gccatcctct cggtgcaggt gctggcgaag
     2461 aacccggggg cgatggccgt ctgggcattc gtcatcgtgc tgctcaccgg catcgggctc
     2521 gccaccgcct tcgtgggcct cgtgctcacc atgcccgtca tcgggcacgc cacgtggcac
     2581 gcctaccgcg actgcgtcgc gtggggcgaa ggagacccgc catgaccgag caggatcgcg
     2641 ccgacaaccc gatgggcacc gacgggttcg agttcatcga gtacacggct cccgacccca
     2701 aactgctggg cgcgctcttc gagcagctcg gcttcacgct cgtggccacg catcgctcca
     2761 aggacgtggc gctctaccga caggggggcg tcaacttcat cgtgaaccgg gagcccgaca
     2821 gcttcgcgca gtcgttcgcg cgcgtgcatg ggccttccgc gtgcgccttc gccatccgcg
     2881 tcaggaacgc agccaaggcc tggcagcgcg cgctcgaact gggcgcgaag ccgcaccacg
     2941 gcccggtggg tcccatggag ctcaacatcc cggcggtcaa gggcatcggc ggcagcctca
     3001 tctacctcgt cgatcgctat ccgggcaacg gcggcaacct ttccatctac gacgtcgact
     3061 tcgtgccggt gga
//
LOCUS       DASUEV010000338         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1697686, whole genome shotgun sequence.
ACCESSION   DASUEV010000338 DASUEV010000000
VERSION     DASUEV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697686"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1200)
                     /locus_tag="VFV55_10710"
     CDS             complement(136..1200)
                     /locus_tag="VFV55_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944816.1"
                     /translation="MPARKILILGASYGSLLGVKLALAGHIVKLVCLPAEAALINSKG
                     AIVRLPVKGREGLVEVNSRSLPGSITADGPDAVDPAAFDLVALAMQEPQYRSPGVREL
                     LTAVGKSRVPCMSIMNMPPMPYLRRIPGVDAQKCIDCYTDASVWDAFEPRFMTLCSPD
                     PQAFRPPEDEVNVLQVRLPTNFKAARFESDAHTALLRELAADIEAARFDSGGEKIELP
                     VKLKVHDSVFVPLAKWAMLLAGNYRCVQADSVRSIKEAVHGDIAATRAVYEWVVKLCV
                     SLGADPGDLVPFEKYANAALSLGSPSSAARALANGAQFIERVDRLVQAVAAQKGMRNA
                     SVDQTVALVDEWLVRNRRKG"
     gene            complement(1368..1958)
                     /locus_tag="VFV55_10715"
     CDS             complement(1368..1958)
                     /locus_tag="VFV55_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4944817.1"
                     /translation="MRAFLAALLAAAAPALAAPPDAQFLEEGRRVYNFRCYFCHGYSG
                     DARTLAATMLPTRPRAFTGQSAAGMPPARIERAVRHGLPGTAMKSFAGTLSDGEIAAV
                     AAFVHDEFVVRRARNTRYHTAENGWPEHERYARAFPFAEGRIPVDAPEASLGKDERAG
                     RRLFLSTCVTCHDRAAARDPGPAWERAPARQSAHAQ"
     gene            complement(1955..>2280)
                     /locus_tag="VFV55_10720"
     CDS             complement(1955..>2280)
                     /locus_tag="VFV55_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4944818.1"
                     /translation="LRGRTLYGHYCAACHGATGRGLEAGAIDREGVRPGPDLTKLPGL
                     SRERVIAAIAVGKASAGKPGFAQQMSPADIEAVTEYVRQSLLALSPGVSGTFAHGGRA
                     SDGKR"
BASE COUNT          348 a          772 c          811 g          349 t
ORIGIN      
        1 ctgggcgcgg gtcgcggtga atgggctcaa ggatctcgag gcgaactcgc cgctgccggg
       61 aaacctgcag cccggacaga agctcgctcc ccgctagcct cggcggggag cgtcaccgcg
      121 ttctcgcgcg cgacgtcaac ccttgcggcg attgcgcacc agccattcgt cgacgagggc
      181 cacggtctgg tccaccgagg cattgcgcat tcccttctgc gccgcgacgg cctgcaccag
      241 gcggtcgaca cgctcgatga actgcgcgcc gttcgcaagc gcgcgggccg cggacgaggg
      301 gctccccagc gacagtgccg cgttggcgta cttctcgaaa ggcacgaggt cgcccgggtc
      361 ggcgccgagc gacacgcaga gcttcaccac ccattcgtac acggcgcggg tggccgcgat
      421 gtcgccatgc accgcctcct tgatggaacg cacggaatcg gcctgcacgc agcgatagtt
      481 gcccgcgagg agcatggccc actttgcgag tggcacgaac accgagtcgt gcaccttgag
      541 cttcaccggc agctcgatct tctcgccccc cgagtcgaag cgggcggcct cgatgtccgc
      601 ggcgagctcg cgaaggaggg cggtgtgcgc atccgactcg aaccgcgctg ccttgaagtt
      661 ggtcggcaaa cgcacctgca gcacgtttac ctcgtcctcg ggcgggcgaa atgcctgggg
      721 gtccgggctg cacagggtca tgaaccgtgg ctcgaacgcg tcccataccg acgcgtccgt
      781 gtagcagtcg atgcatttct gcgcatcgac gccggggatg cgcctgagat agggcatcgg
      841 cggcatgttc atgatcgaca tgcacggcac ccgggacttg ccgacggccg tgagcaactc
      901 gcgcacgccg ggcgagcggt attgcggctc ctgcatcgcg agcgcgacga ggtcgaaggc
      961 ggcgggatct accgcgtccg gcccgtcggc ggtgatgctt cccggaaggg aacgcgagtt
     1021 cacctcgacg agcccttcac ggccctttac cggcaggcgc acgatcgcgc ccttcgaatt
     1081 gatgagcgcc gcctccgcgg gaaggcagac gagcttcacg atgtgccccg cgagcgcgag
     1141 cttcacgccc agcagcgaac cgtacgaggc gcccaggatc aggatcttcc gagcaggcat
     1201 gggttctcct ccttagcttg tggcggcgcg gatgggtggc gaggtctcgt ggacgcccgc
     1261 gtcccgtacg cattccggtg ttgtactgat atggtaagcc gatcgcgcgc aggcgttgcc
     1321 tgacccgcgg atgcaacggc tgttacccga ctggccgaag tccggtgcta ttgcgcgtgc
     1381 gcagactggc gcgcgggggc cctttcccag gcggggcccg ggtcgcgcgc ggcggcgcgg
     1441 tcatggcagg taacgcacgt cgaaaggaaa agccgcctgc ccgcgcgctc gtccttgccg
     1501 agcgaggcct ccggcgcgtc gaccgggatg cgaccttcgg cgaacggaaa ggcgcgggcg
     1561 tagcgttcgt gctccggcca gccgttttcc gccgtgtggt agcgcgtgtt gcgcgcgcgc
     1621 cgcaccacga actcgtcgtg gacgaaagcg gccacggccg cgatctcgcc atcggagagc
     1681 gttccggcaa aggacttcat ggccgtgcct ggcaggccat ggcgaaccgc cctctcgatg
     1741 cgcgccggcg gcatgcccgc ggccgattga ccggtgaacg cgcgcggcct tgtcggaagc
     1801 atcgtggcgg cgagcgtgcg agcgtcgccg gagtaaccgt ggcagaagta gcaacggaag
     1861 ttgtagacgc gccggccttc ttcgaggaat tgcgcgtcgg gcggcgcggc cagcgcgggt
     1921 gccgcggccg ccaggagagc ggccaggaat gccctcatcg ctttccgtcc gaggcccttc
     1981 cgccgtgcgc aaacgtgccg gaaacgccgg gtgacagcgc gagaagcgac tggcggacgt
     2041 attcggtgac cgcctcgatg tcggccggcg acatctgctg cgcgaagccg ggcttgccgg
     2101 ccgaagcctt gcccaccgcg atggcggcga tcacgcgttc gcggctgagg ccgggcagct
     2161 tcgtgaggtc gggccccggg cgcacgcctt cccggtcgat cgcgcccgcc tcgaggccgc
     2221 gcccggtcgc cccatggcac gcggcgcagt agtggccgta gagggtgcgc ccgcgcagcg
//
LOCUS       DASUEV010000339         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1697998, whole genome shotgun sequence.
ACCESSION   DASUEV010000339 DASUEV010000000
VERSION     DASUEV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697998"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFV55_10725"
     CDS             <1..415
                     /locus_tag="VFV55_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX4944819.1"
                     /translation="WAIRRALHPDPLGRLTRAAEIGGARGVIREYRTIKPWYPPVTLN
                     EFLLNRVGYGLLQSGKVDDAIAVFRLNVEEHATAANTHDSLGEAYATRGNWALAIRSY
                     ERSLQLDRRNRNAAQRLEQLRKCMGGAVGDCPKVN"
     gene            complement(458..1537)
                     /locus_tag="VFV55_10730"
     CDS             complement(458..1537)
                     /locus_tag="VFV55_10730"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944820.1"
                     /translation="MTGAALTTCRSDSVTCGRREFLVGAFLVTISGASLAQAPGKVHV
                     IGVLLPWNAAPRDGSPPPAPRWLDEALARNGFVVGRNVRIETAFGNVRIEREFDERER
                     ERRDSLVAVAARKLLALDPDVIFVASAAGIAALQALTKSVPIVFANQVDPVQAGFVRE
                     LRRPGGNVTGVALLYEELAEKRIELVRQLLPGAKRIALIADFPLARERNRTFPSRLEA
                     AASRLGLLLIRGDVSAHGGDLAATLRHVLESRPDAFMAFGTIPSAPDRFEQFLKFEKT
                     HRIPFIGDGAEDGRGVVSYGADYDDHFRRAAEMIVRILRGASPADTPVDRDTRFLLVV
                     DLPLAKQIGIEIPPSILLRADRIIR"
     gene            complement(1551..2522)
                     /locus_tag="VFV55_10735"
     CDS             complement(1551..2522)
                     /locus_tag="VFV55_10735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX4944821.1"
                     /translation="MTLALRIALAIVLAAGCGAARAQTDYPNKPIRMIIPLAAASAVD
                     NAARIIAQRMSESLGQPIVIENQPGAAGLIGAERLAKSTPDGYTIGAFNDSILTMLPN
                     MHDKLAWDPVRDFDPVSLVGTIEWGLITGNDTPYKTAADVIAAAKAKPGKLDYSSGGN
                     GSPQHVGMALFASTSGISLQHVPYKGATQAAVGVAAGEVPVGMQGLGTAAGLVRSGKL
                     RLIGVSTPARLASFPDVPTISESGLPGFEFRSWFVVVAPAGTPKAIVAKLNAEIRKAL
                     AIPEVREKLVAQGFTVLGSSPEELGAATKDQLARYAKLMKQAGIKAE"
     gene            complement(2553..>3327)
                     /locus_tag="VFV55_10740"
     CDS             complement(2553..>3327)
                     /locus_tag="VFV55_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4944822.1"
                     /translation="KRAAELAIAKARDAGVAWVGAHWSNHAGPASLYARMPLAHDMVG
                     LYVAVGNANHLPPWGGLDMLLSTNPIAVAVPTLEEPPVVLDMATTVAAYGKVKTYAQR
                     GLAMPEGWMIDRDGKPLTDPKRADEGFLLPVGGYKGYGLALVFGLLAGTLNGAAMGKD
                     VIDFNADSSSATNTGQAIVAIDPAAFGDVTQFKRRVDAVVRDLRASRRMPGVERIWMP
                     GEQSHMKRAMAAREGIALSPALRKALDTVADDLGIARLA"
BASE COUNT          546 a         1087 c         1155 g          539 t
ORIGIN      
        1 ctgggcgatc cgccgtgctc tccatcccga ccccctcggg cgactgacca gggcggccga
       61 gataggtggg gcccgcggcg tgatccggga gtaccggacg atcaagccct ggtacccgcc
      121 cgtgacgctc aacgaattcc tcctcaatcg cgtgggatac ggcctgctcc aatcggggaa
      181 ggtggacgat gcgattgccg tgttccgcct gaatgtggaa gagcacgcga ccgcggcgaa
      241 cacccatgac agcctgggcg aggcgtacgc gacgcgtggc aattgggcgc tcgccatcag
      301 gagttacgag cgctcgctgc agctggatcg tcggaacagg aacgcggcgc aacggctcga
      361 gcagctgcga aaatgcatgg gcggcgccgt gggcgattgc cccaaggtga attagatccg
      421 gatcacttcg aagaatgcct gccgccgggc cgtccggtca tcgaatgata cggtcggcgc
      481 gcaacaggat cgacggcgga atctcgatgc cgatctgctt cgcgaggggg aggtcgacga
      541 ccaggaggaa acgcgtatcc cgatccaccg gagtatccgc cggacttgcg ccgcgcagga
      601 tccggacgat catttccgcg gcgcgccgga aatgatcgtc gtagtccgca ccgtaggaca
      661 ccactccccg accatcttcc gctccgtcgc cgatgaacgg tatccgatgc gtcttctcga
      721 acttcaggaa ttgctcgaat cggtcgggag cgctgggaat ggtgccgaac gccatgaatg
      781 catcgggacg cgactcgaga acgtgcctca gggtggcagc gaggtcgccg ccatgcgcgg
      841 agacgtcccc gcggatgagc aggagtccga gacggcttgc ggccgcttca aggcgggaag
      901 ggaaagtcct gttgcgctcg cgggccaggg gaaaatccgc gatgagcgcg atgcgcttcg
      961 cgccgggcag cagttgccgg acgagttcga tgcgcttttc cgcaagctcc tcatacagca
     1021 gcgcgacgcc cgtgacgttt ccgccgggac gacgcaactc ccggacgaag ccggcctgga
     1081 caggatcgac ctggttggcg aatacgatcg ggaccgactt cgtcagtgcc tgaagcgctg
     1141 cgattcctgc ggcgctggcg acaaagatca catcgggatc gagggcaaga agcttccggg
     1201 cggctaccgc gacaagcgaa tcgcgccgct cacgctcgcg ttcgtcgaac tcacgctcga
     1261 tgcggacatt cccgaatgca gtttcgatcc gaacgttcct gccaacgaca aatccattgc
     1321 gtgcaagggc ttcgtcgagc catcgggggg cgggaggagg tgacccatcc cgtggagccg
     1381 cgttccaagg gagcaggacg ccgatcacat ggactttccc gggcgcttgg gcgagcgatg
     1441 caccggagat tgtcaccaga aaagcgccga cgagaaattc gcggcgaccg caggtgacgg
     1501 aatcgctccg gcacgtcgtg agtgccgccc cggtcacgga tcgagcgcgc ctattccgcc
     1561 ttgatgcccg cctgcttcat gagcttcgcg tagcgggcga gctggtcctt cgtggccgcg
     1621 cccagctcct cgggtgacga gccgagcacc gtgaagccct gcgcgacgag cttctcgcgc
     1681 acctctggta tcgccagcgc cttgcggatc tccgcgttca gcttcgcgac gatcgccttg
     1741 ggcgttcccg cgggcgccac caccacgaac cacgagcgga attcgaaacc cggaaggccc
     1801 gattccgaga tcgtgggcac gtccgggaag gaggcgagac gcgccggcgt ggacacaccg
     1861 atcaggcgca gcttccccga tcgcaccagc cccgcggccg tgcccagccc ctgcatgccg
     1921 acggggacct cgcccgcggc cacgcccacg gcggcctgcg tggcgccctt gtagggcacg
     1981 tgctggagcg agatgcccga ggtgctcgcg aagagcgcca tccccacatg ctgcgggctc
     2041 ccgttgccgc cggagctgta gtcgagcttg ccgggcttcg ctttggccgc tgcaatcacg
     2101 tcggccgccg tcttgtaggg agtgtcgttg ccggtgatga ggccccattc gatggtgccc
     2161 acgagcgaga cggggtcgaa gtccctgacc gggtcccagg ccagcttgtc gtgcatgttg
     2221 ggcagcatcg tgaggatgct gtcgttgaac gcgccgatcg tgtagccgtc cggcgtggac
     2281 ttcgcgaggc gctcggcgcc gatgaggccc gcggccccgg gctggttttc gatcacgatc
     2341 ggctggccga ggctctcgga catgcgctgc gcgatgatgc gcgccgcgtt gtccacggcg
     2401 ctggccgccg cgagcggaat gatcatgcga atgggcttgt tcggataatc cgtctgggcg
     2461 cgggcggcgc cacagcccgc ggcgaggacg atggcaaggg cgatgcgcag ggcgagcgtc
     2521 attccggttc tccttgaagg ggcgtgccgc gatcaggcga ggcgcgcgat gccgaggtcg
     2581 tcggccacgg tgtcgagggc cttgcgcagc gccggcgaaa gggcgatgcc ctcgcgcgcg
     2641 gccattgcgc gtttcatgtg gctctgttcg ccgggcatcc agatgcgttc cacccccggc
     2701 atgcggcggc tcgcgcgaag gtcgcgcacg acggcatcca cgcgccgctt gaattgcgtg
     2761 acgtcgccga aggcggccgg gtcgatggcg acgatcgcct gcccggtgtt cgtggcgctc
     2821 gacgaatcgg cgttgaaatc gatcacgtcc ttgcccatgg ccgcaccgtt gagcgtgccc
     2881 gcgagcagcc cgaagacgag cgccaggccg tagcccttgt agccgccgac cggcaggagg
     2941 aaaccctcgt ccgcgcgctt cgggtcggta aggggtttgc cgtcgcggtc gatcatccag
     3001 ccttcgggca tggcgaggcc gcgctgcgcg taggtcttca cctttccgta ggcagcgacc
     3061 gtggtcgcca tgtcgagcac cacgggcggc tcctcgagcg tgggcacggc gaccgcgatg
     3121 ggattggtgg agagcagcat gtccagcccg ccccacggcg gcaggtgatt ggcgttgccc
     3181 actgcgacgt agagcccgac catgtcgtgc gcaagcggca tgcgcgcgta gagcgaagcc
     3241 ggccctgcgt ggttgctcca gtgcgccccc acccaggcga cgcccgcgtc ccgtgccttg
     3301 gcgatcgcga gctcggcggc gcgcttc
//
LOCUS       DASUEV010000340         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_662981, whole genome shotgun sequence.
ACCESSION   DASUEV010000340 DASUEV010000000
VERSION     DASUEV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662981"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1074)
                     /locus_tag="VFV55_10745"
     CDS             complement(97..1074)
                     /locus_tag="VFV55_10745"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX4944823.1"
                     /translation="MNTTRTLLGAFSACLLAATAAVAQTEIKFGHVGEPGSLFAQSAE
                     EFAKRANAKLGSKAKVVVYGSSQLGGDKELVQKLKLGTVDIALPSTVMSSEADLFGVF
                     EMPYLVKDRKHMGRIEKDVFWPKIEPTAEKKGLKVIAVWENGYRHITNSKRPINTPED
                     LKGIKLRVPEGKWRVKMFQAYGANPSPMKFSEVFTALQTGVMDGQENPFTQIYSAKFQ
                     EVQKYLSLTGHVYTPAYATVGTKKWATLPPDVRKILEDTAKETQAYVYQMAEKGESEL
                     LDKIKAAGVQVNTPNKDAFIAASKPVYEEFAKEVAGSKDVIDKAIALGK"
     gene            1403..1591
                     /locus_tag="VFV55_10750"
     CDS             1403..1591
                     /locus_tag="VFV55_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944824.1"
                     /translation="MKLTTALVTFVLAAAATTAWGHPGHGAEGQAHHFVDLLALGGIA
                     ALLAFGWTGRKDGGKDHD"
     gene            1584..2669
                     /locus_tag="VFV55_10755"
     CDS             1584..2669
                     /locus_tag="VFV55_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEX4944825.1"
                     /translation="MTETFYDVLRRQGITRRSFMKFCSLTAASLGLGPTLAPQIAHAL
                     ETKPRTPVLWLHGLECTCCTESFIRSAHPLAKDVILSMISLDYDDTIMAAAGHQAEAA
                     LEEVMTKYKGNYILACEGNPPLKDDGIYCIPGGKPYVEKLKRVAKDAKAVIAWGSCAS
                     WGCVQAAKPNPTGAVPIDKVITDKPIIKVPGCPPIAEVMSAVVTYVVAFDRIPELDRQ
                     GRPKMFYSQRIHDKCYRRPHFDAGQFVEKWDDEGAKMGYCLYKMGCKGPTTYNACSST
                     RWNEGTSFPIQSGHGCIGCSEDGFWDKGSFYERLTSIPAFGVEANADKVGLVAAGVVG
                     AAVVAHAAVTAIRNASEKRAPTKSEEK"
     gene            2672..>3148
                     /locus_tag="VFV55_10760"
     CDS             2672..>3148
                     /locus_tag="VFV55_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX4944826.1"
                     /translation="MAVIETQGFKLDNSGKRVVVDPITRIEGHLRVEVNLDAKNVITN
                     AVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGVHALASVRCVEDALGIAIPDNA
                     NIIRNLMHATLYAQDHLVHFYHLHALDWVDVVSALKADPKKTSELAQSISGWPNSSP"
BASE COUNT          513 a         1066 c         1023 g          546 t
ORIGIN      
        1 cgccgccacc tcggcggcga gcgcggccat gagcacgatg cagatccact cgaggaatcg
       61 ctcgtatccc cggcggaacg actgcagcgt catcgcctac ttgcccaggg cgatggcttt
      121 gtcgatcacg tccttggagc cggcgacttc cttggcgaac tcctcgtaga cgggcttcga
      181 ggcggcgatg aaggcgtcct tgttgggcgt gttcacctgc accccggcgg ccttgatctt
      241 gtcgagcagc tcgctttcgc ccttctcggc catctggtag acgtaggcct gcgtctcctt
      301 cgccgtgtcc tcgaggatct tcctcacgtc cggcggcagc gtggcccatt tcttcgtgcc
      361 gaccgtggcg taggccggcg tgtagacgtg gccggtgagg gagaggtact tctgcacttc
      421 ctggaacttg gcgctgtaga tctgggtgaa ggggttctcc tggccgtcca tcacgcccgt
      481 ttgcagggcg gtgaacacct ccgagaactt catcgggctg gggttcgccc cgtaggcctg
      541 gaacatcttc acgcgccact tgccctcggg cacgcgcagc ttgatgccct tgaggtcctc
      601 gggcgtgttg atggggcgct tggaattggt gatgtggcgg tagccgttct cccagactgc
      661 gatgaccttg agacccttct tctcggcggt gggctcgatc ttcggccaga agacgtcctt
      721 ctcgatgcgg cccatgtgct tcctgtcctt cacgaggtag ggcatctcga agaccccgaa
      781 gaggtccgcc tcggaggaca tgacggtgga tggcagggcg atgtcgaccg tgccgagctt
      841 gagcttctgg acgagctcct tgtcgccgcc gagctggctg gagccgtaga cgacgacctt
      901 cgccttgctg cccagcttgg cgttggctcg cttcgcgaac tcctcggcgg actgggcgaa
      961 gagcgagcct ggctcgccga cgtggccgaa cttgatctcg gtctgggcga cggcggcggt
     1021 ggcggcaaga aggcatgcgg aaaatgcgcc cagcagggtg cgggtggtgt tcatgagctc
     1081 ctcctcgtta tggatgtgtg gctccggcgc gcgctctcac gtccgggctc cccgtagtct
     1141 aggcatatcg cgacagcggc gtcgatgacc tccgccggtg cgcgaccctg ctgcccggga
     1201 aaaggggacg caaccctttt gcagcttttt tccgccgcct gcattggcgg gaaaagggtt
     1261 gcgtcccctg cggccggtgt aacggtcagc gttacgcgcc acgcgcctcg ccccgtgtcg
     1321 ttgatgtagg tcaaaagggg cacccgggcg gcggcggaca gtccgcttca tgtccggtac
     1381 ccctaccgtc cgaggaggcc ccatgaaact caccaccgcg ctcgtcacgt tcgtcctcgc
     1441 cgccgcggct accaccgcct ggggccaccc cggccacggc gccgaagggc aggcccacca
     1501 tttcgtcgac ctgctcgccc tcggcggcat cgcggccctg ctggcgttcg gatggacggg
     1561 ccggaaagac ggaggcaagg accatgactg agaccttcta cgacgtgctc cgccgccagg
     1621 gcatcacgcg gcgcagcttc atgaagttct gcagcctcac ggccgcctcc ctcggcctcg
     1681 ggccgacgct cgccccgcag atcgcgcacg cgctcgagac caagccgcgc accccggtgc
     1741 tgtggctgca cggcctggaa tgcacctgct gcaccgaaag cttcatccgc tcggcgcacc
     1801 cgctcgccaa ggacgtgatc ctgtccatga tctcgctcga ttacgacgac acgatcatgg
     1861 cggccgccgg ccaccaggcc gaggcggcgc tcgaggaggt gatgaccaag tacaagggca
     1921 actacatcct cgcctgcgaa ggcaatcccc cgctcaagga cgacggcatc tattgcatcc
     1981 cgggcggcaa gccgtacgtc gagaagctca agcgcgtcgc gaaggacgcc aaggccgtca
     2041 tcgcctgggg ttcctgcgcc tcctggggct gcgtgcaggc cgccaagccc aacccgacgg
     2101 gcgcggtgcc catcgacaag gtcatcaccg acaagccgat catcaaggtg ccgggctgcc
     2161 cgccgatcgc ggaagtcatg agcgcggtgg ttacctacgt cgtcgccttc gaccgcatcc
     2221 ccgagctcga ccgccagggc cggccgaaga tgttctacag ccagaggatc cacgacaagt
     2281 gctaccgccg gccgcacttc gatgccggcc agttcgtcga gaaatgggac gacgagggcg
     2341 cgaagatggg ctactgcctc tacaagatgg gctgcaaggg gccgacgacg tacaacgcct
     2401 gctcgagcac gcgctggaac gagggcacgt ccttcccgat ccagtcgggg cacggctgca
     2461 tcggctgctc cgaggacggg ttctgggaca agggctcgtt ctacgagcgc ctcaccagca
     2521 tcccggcctt cggcgtcgaa gccaatgccg acaaggtcgg cctggtggcc gccggtgtcg
     2581 tcggcgcggc cgtcgtggcg cacgccgcgg tgaccgccat ccggaacgct tccgagaagc
     2641 gcgcacccac gaagagcgag gagaagtgac catggccgtc atcgagacgc aaggcttcaa
     2701 gctcgacaac agcggcaagc gggtcgtcgt cgaccccatc acccgcatcg aggggcacct
     2761 gcgcgtggag gtgaacctcg acgcgaagaa cgtgatcacc aacgccgttt ccacgggcac
     2821 catgtggcgc gggctggagg tgatcctcaa gggccgcgac ccgcgcgacg cgtgggcctt
     2881 cgtggagcgc atctgcggcg tgtgcacggg cgtgcatgcg ctcgcctccg tgcgctgcgt
     2941 cgaggacgcg ctcggcatcg cgatcccgga caacgcgaac atcatccgca acctgatgca
     3001 tgcgacgctc tacgcccagg accacctcgt gcatttctac cacctgcacg ccctcgactg
     3061 ggtggacgtg gtctcggcgc tgaaggccga tccgaagaag acgtccgaac tcgcgcagtc
     3121 catctccggc tggcccaact cctcgccg
//
LOCUS       DASUEV010000341         7128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_322156, whole genome shotgun sequence.
ACCESSION   DASUEV010000341 DASUEV010000000
VERSION     DASUEV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322156"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFV55_10765"
     CDS             complement(<1..188)
                     /locus_tag="VFV55_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX4944827.1"
                     /translation="MHKRIWTGLVAALAILAATNTGAAYPEKPIQLIVPYGAGGSTDL
                     LARAIAQVAPKYFTQPLVV"
     gene            complement(281..1786)
                     /locus_tag="VFV55_10770"
     CDS             complement(281..1786)
                     /locus_tag="VFV55_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX4944828.1"
                     /translation="MFDIAGLWQGFQIAFTPVNLLWCLIGCALGTAVGVLPGLGPAAT
                     IALLLPISMKMGSPVTAIILMSGIFYGAMYGGSTTSILLKIPGEAASVVTCIDGYEMA
                     KMGRAGAALGVAAIGSFVAGSIGLVGLTMMAPPLAEAALKFGPPEYFSLTVLGLLLAT
                     YLAGDAPLKGLIMAILGLLLASVGLDPLSGAVRFDFGVADLQSNIDFVTLAMGLFGVG
                     EILVSLEKTEAAGILTTSIKNVFPTLADLARSGWAMVRGAVIGFFVGILPGGGAVVSS
                     LISYAVEKKVSKHPEEFGHGAIEGVAGPEAANNAAASSSFIPLLTLGIPGNASTAMIF
                     AALLIHGITPGPFLIKEHADVFWGVVASMYIGNVMLLILNLPLVGMWAQLLRVPYAFL
                     APLIMVLTLVGVYSVNNSVFDIWVMLCMGVFGYIARKLKFDLGPLLLAFVLGPIMERS
                     LRQALLMSDGHFQVFFTRPISGALMGVAILFVLFNIWAARRKKREARESAT"
     gene            complement(1805..2254)
                     /locus_tag="VFV55_10775"
     CDS             complement(1805..2254)
                     /locus_tag="VFV55_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX4944829.1"
                     /translation="MKSWQRYAGVFLLALAALVIQQSVWVLRLLDHGQPGSGFMPFGL
                     GVILAILAICLIATNLGHEERRVPFWEPKAWLHPLLAVIITAIYVVVFDDIGAITSVV
                     VLVTFWLAFVEHKSLAVSVVTGVATGLVVYLVFDRFLRTPFPRGMLF"
     gene            complement(2260..3033)
                     /locus_tag="VFV55_10780"
     CDS             complement(2260..3033)
                     /locus_tag="VFV55_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX4944830.1"
                     /translation="MRENGIRTIWKAGGAVVNGWLAIPSSFSAETMAHQGWDSLTIDM
                     QHGVIDYGDAVAMLTAIATTGTVPVVRVPWLDPGILMKMLDAGAYALICPMVNNRDDA
                     EKLVAATHYPPRGSRSMGPIRAQLYGGPDYQKQANDTIVVFAMIETRQALDNLDDILL
                     VKGLDAVYIGPADLSLALGCNPTFDDVDPPVAEAIDLILAKAKEHGVVAGIHNGSPEG
                     ALRRIAKGFQFVTIGSDARLMAAGAKQVVDNMRAGLKKG"
     gene            complement(3277..4167)
                     /gene="glxR"
                     /locus_tag="VFV55_10785"
     CDS             complement(3277..4167)
                     /gene="glxR"
                     /locus_tag="VFV55_10785"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX4944831.1"
                     /translation="MSKIGFIGLGIMGAPMAGHLQAGGHELHVFTIGPVPDALVRGGA
                     KVRANSAEVAKNADIIIVMVPDTPDVEKVLFAPGGVAEGLSAGKIVVDMSSIAPIETK
                     EFAKRINALGCEYLDAPVSGGEVGAKAASLTIMVGGSEATFAKVKPIFEMMGKNITLV
                     GGNGDGQTCKVANQIIVALTIEAVGEALLFASKAGADPAKVRQALMGGFASSRILEVH
                     GERMVKRTFDPGFRIELHQKDLSLALGSARKMGVSLPNTATCQELFNACAAHGGKAWD
                     HSAMVRALEKLANFEIGQKP"
     gene            complement(4203..5558)
                     /gene="gudD"
                     /locus_tag="VFV55_10790"
     CDS             complement(4203..5558)
                     /gene="gudD"
                     /locus_tag="VFV55_10790"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR03247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HEX4944832.1"
                     /translation="MSPPPLQSPARGAPAVTELRVVPVAGHDSMLLNLSGAHGPFFTR
                     NVVIIRDSAGNTGVGEVPGGEKIRATIEDARPLVVGRTVSSVNAVLQDMRRRFADRDA
                     GGRGLQTFDLRTTIHAMTAVESAMLDLLGQFLGVPIADLLGEGRQRSQVEVLGYLFYV
                     GDRTRTDLPYRSEPDASDDWLRLRNEEATTAESIVRQAEAAHRRYGFNDFKLKGGVLR
                     GEEEVEAIRALHERFPRARVTLDPNGGWLLEDAIRLCRDLHGVMAYAEDPCGAEGGFS
                     GREVMAEFRRATGLPTATNMVATDWRQLVHALQLQAVDIPLADPHFWTMQGSVRVAQT
                     CRDWGLTWGSHSNNHFDISLAMVTHVAAAAPGRVTAIDTHWIWQDGQLLTKEPYLIRG
                     GLLEVRERPGLGVELDPAQLDRAHELYRAHGLGARDDALAMRYLVPGWQFDNKRPSLV
                     R"
     gene            5733..6800
                     /locus_tag="VFV55_10795"
     CDS             5733..6800
                     /locus_tag="VFV55_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX4944833.1"
                     /translation="MARTRSARKQPRKPRRGSVQSTARAASGKAVTIRDVAKVAGVSP
                     ITVSRALSRPELVTPETLERIRAVVSRTGYVRNVMAGSLRSRRSRIIAAVFPQVANTM
                     FVDTIQALMDRLRQDGYQVLLGLSSYQMREDELIAGILGRQPEGIFLTGISHAPETRR
                     RLIASHIPVVEAWDLTPTPIDMLVGFSHEKVGAAMARHLLGKGHREIGLVWSTDIRAG
                     MRRQGFLSVLAENGITQAPTSLRPAPATFQRGREGLAELLGSGARVSAVACGSDTLAQ
                     GVIAEAQDRGIRIPGDIAVMGFGNLDFAAHTTPALTTIGIDKAAIGRLAAEALIARIE
                     GREVREKIVDVGFELIEREST"
     gene            complement(6823..>7128)
                     /locus_tag="VFV55_10800"
     CDS             complement(6823..>7128)
                     /locus_tag="VFV55_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944834.1"
                     /translation="APTDPGLVLGPVLHSFDGKGKGDFNSGRFRDAELDRLIERSEVE
                     MDPARRAALMEEALQRVRTNAYTIPLHRQVIPWAVRKGVTVFHRPDNYLEMTWVKID"
BASE COUNT         1198 a         2373 c         2388 g         1169 t
ORIGIN      
        1 acgacgagcg gctgcgtgaa atacttcggc gcgacctggg cgatcgctcg cgccaggagg
       61 tccgtggagc cgcccgcgcc atacggcacg atgagctgga tcggcttttc cggataggct
      121 gccccggtgt tcgttgctgc aagaatggca agggctgcga ccagccccgt ccagattcgc
      181 ttgtgcattt gagactcctc ttgtctgact actcgaagcg tgaatacaac tgcggtcagc
      241 gtgcgccggg acccgccatg aacggtcccg tccgcaccgc ctaggttgca ctttcccggg
      301 cttcgcgctt cttccttcgc gccgcccaga tgttgaagag cacgaacagg atcgcaacgc
      361 ccatcagcgc gccggagatc ggccgcgtga agaagacctg gaaatggccg tcgctcatga
      421 gcagggcctg gcgaagcgaa cgctccatga tcgggccgag cacgaaggcc aggagcagcg
      481 gcccgaggtc gaacttcagc ttgcgtgcga tgtagccgaa gacccccatg cacagcatga
      541 cccagatgtc gaagacgctg ttgttgacgc tgtagacccc gaccagggtg aggaccatga
      601 tcagcggcgc caggaaggca taggggacgc gcagaagctg cgcccacatg ccgacgaggg
      661 ggaggttcag gatcagcagc atcacgttgc cgatgtacat cgacgccacc acgccccaga
      721 agacgtccgc gtgctccttg atcaggaacg gtcccggcgt gatgccgtgg atgagcaggg
      781 ccgcgaagat catcgccgtg gatgcgttcc cgggaatgcc gagggtgagc agcgggatga
      841 aggacgacga ggccgcggcg ttgttggcgg cctcgggtcc ggcgacaccc tcgatggccc
      901 cgtgcccgaa ctcctccggg tgcttcgaga ctttcttctc cacggcatag gagatgagcg
      961 aggacacgac cgcgccgccg ccgggcagta tgccgacgaa aaagccgatc accgctcctc
     1021 gcaccatggc ccagccgctt cgcgcgagat cggccagcgt cggaaacacg ttcttgatgg
     1081 aggtggtgag gatgccggcg gcctcggtct tctcgaggct gaccaggatc tcgccgacgc
     1141 cgaacagccc catcgccagg gtcacgaaat cgatgttgct ttgcaggtcg gcgacgccga
     1201 aatcgaagcg aaccgcaccc gagagcgggt cgaggccgac ggacgccagg agcaggccga
     1261 ggatcgccat gatcagcccc ttcagcggcg catccccagc aaggtaggtc gcgaggagca
     1321 agcccagcac cgtcagcgag aaatactcgg gagggccgaa cttcagggcg gcctcggcca
     1381 gcggcggcgc catcatggtc agtcccacca ggccgatgct gccagcgacg aaggagccga
     1441 tagcggccac gccgagggcc gcgccggcgc ggcccatctt ggccatctcg tacccgtcga
     1501 tgcaggtgac gaccgaagcc gcttcgccgg ggatcttcag gaggatggaa gtcgtcgacc
     1561 cgccgtacat ggccccgtag aagatgccgg acatgaggat gatggcggtg acgggcgagc
     1621 ccatcttcat gctgatgggc agcagcaggg cgatggtggc cgccggaccc agccccggca
     1681 ggacgccgac cgcggtgccg agggcgcaac cgatcaggca ccagaggaga ttgaccgggg
     1741 tgaaggcgat ctggaagcct tgccacagtc cggcgatgtc aaacacgtcc ttgatctccc
     1801 tggttcagaa cagcatgccc ctggggaacg gggtgcgcag gaagcgatcg aagaccaggt
     1861 agaccacgag gccggtcgcg acgccggtga cgaccgatac ggcgaggctc ttgtgctcca
     1921 cgaaggcgag ccagaacgtg acgagcacca cgacgctggt tatcgcgccg atgtcgtcga
     1981 agacgaccac gtagatcgcg gtgatgatca ccgccaggag cggatgcagc cacgccttgg
     2041 gctcccagaa gggaacccgc cgctcctcgt gcccgagatt ggtggcgatg agacagatgg
     2101 ccaatatcgc gaggatcacc cccaggccga aaggcatgaa gcccgagccg ggctggccat
     2161 ggtcgagcag gcgcagcacc cagaccgact gctggatcac gagcgcggcg agcgcaagga
     2221 gaaacacgcc cgcataccgc tgccaggatt tcatcgcggt catcccttct tcaggcccgc
     2281 ccgcatgttg tccacgacct gcttcgcgcc cgcggccatg agccgcgcat cggagccgat
     2341 ggtcacgaac tggaagcctt tcgcgatgcg ccggagcgcg ccttcgggcg acccgttgtg
     2401 tatgcccgcg acgacgccgt gttccttcgc cttcgcgagg atcaggtcga tcgcctcggc
     2461 cacgggcggg tccacgtcgt cgaaagtagg gttgcagccg agcgccagcg agaggtccgc
     2521 cgggccgatg tagacggcat cgagcccctt caccaaaagg atgtcgtcga ggttgtccag
     2581 cgcctggcgc gtctcgatca tcgcgaagac cacgatcgtg tcgttcgcct gcttctggta
     2641 gtcgggcccg ccgtagagct gcgcgcggat ggggcccatg ctgcggctgc cccgcggggg
     2701 gtagtgcgtc gctgccacca gcttctcggc gtcgtcgcgg ttgttgacca tggggcagat
     2761 cagggcgtag gcgcccgcgt ccagcatctt catgaggatg cccgggtcga gccagggaac
     2821 gcgaaccacg ggcacggtgc ccgtcgtggc aatcgccgtg agcatcgcga cggcatcccc
     2881 gtaatcgatg acgccgtgct gcatgtcgat ggtcagcgaa tcccatccct ggtgggccat
     2941 ggtctccgcc gaaaagctgc tggggatggc cagccacccg ttgaccaccg cgccgccggc
     3001 cttccagatc gtgcggatcc cgttctcacg catcatcgcc tccatgaatc acggtcggtt
     3061 gcagtatcga ggagtgggag caaggtgtgg tgagtgtttt tttctttttc ggccgagtca
     3121 atttgcttcg ggcatcgttg cccgagccta tgtcgagatg ctcgcttcat cgaagtcgat
     3181 ggcaagttca ttcggccgaa aaagaaaaag aacactcacc acaccttgct ccagtcgtgc
     3241 cccttgcagg cgccccggga agattccggc aggcgtctat ggtttctgcc cgatctcgaa
     3301 gttggccagc ttctccagcg cccgcaccat cgccgagtgg tcccacgcct tgccgccgtg
     3361 cgcggcgcac gcgttgaaca gctcctggca ggtggccgtg ttgggcaggc tcacgcccat
     3421 cttgcgggcc gagccgagcg cgagcgacag gtccttctgg tgcaattcga tgcggaagcc
     3481 ggggtcgaag gtgcgcttca ccatgcgctc gccgtgcacc tcgaggatgc gcgacgaggc
     3541 gaatccgccc atcagcgcct ggcggacctt ggcgggatcc gcccccgcct tcgatgcgaa
     3601 gagcagcgcc tcgccgaccg cctcgatggt cagcgccacg atgatctggt tggccacctt
     3661 gcacgtctgg ccgtcgccgt tgccgccgac cagggtgatg ttcttgccca tcatctcgaa
     3721 gatcggcttc accttcgcga acgtcgcctc gcttccgccc accatgatgg tgagcgatgc
     3781 ggccttcgcg cccacctcgc cgccggatac cggcgcatcg aggtattcgc agcccagcgc
     3841 gttgatgcgt ttcgcgaatt ccttggtctc gatcggtgcg atggagctca tgtcgacgac
     3901 gatcttgcct gcggaaagtc cctcggcgac gccgcccggc gcgaagagca ccttctccac
     3961 gtccggggta tccggaacca tgacgatgat gatgtccgcg ttcttcgcca cctcggccga
     4021 gttggcgcgc accttggcgc cgccgcggac cagcgcatcg ggcacgggcc cgatggtgaa
     4081 cacgtgcagc tcgtgcccgc ccgcctgaag gtggcccgcc atgggcgcgc ccatgattcc
     4141 caaaccgatg aagccgatct tgctcatctc gatcccccta ttgcgtatcg gacatgtttg
     4201 gctcagcgca ccaggctcgg ccgcttgttg tcgaactgcc agcctggaac aaggtagcgc
     4261 attgcgagag cgtcgtcgcg ggcacccaat ccgtgggccc tgtagagctc gtgcgcccgg
     4321 tcgagctgcg ccggatcgag ctccacgccg aggccgggcc tttcgcgaac ctcgagcagg
     4381 ccaccccgga tcaggtatgg ctccttcgtc aggagctggc cgtcctgcca gatccagtgc
     4441 gtgtcgatcg ccgtgacgcg acctggcgcc gcggcggcca cgtgcgtgac catggcgagc
     4501 gagatgtcga agtggttgtt cgagtgggag ccccaggtga ggccccagtc cctgcaggtc
     4561 tgggccacgc gtaccgatcc ctgcatcgtc cagaagtgcg ggtcggcgag cggtatgtcg
     4621 acggcctgca actgcagggc gtgcacgagc tggcgccagt cggtcgcgac catgttcgtg
     4681 gccgtgggca acccggtggc gcggcggaat tccgccatca cctcgcggcc cgagaatccg
     4741 ccctcggcgc cgcaggggtc ctcggcatag gccatcacgc cgtgcaggtc gcggcaaagg
     4801 cggatcgcgt cctcgagcag ccatcccccg ttggggtcga gcgtgacgcg cgcgcgcgga
     4861 aagcgctcat gcagcgcccg gatcgcttcc acttcctcct cgccccggag cacgccgccc
     4921 ttcagcttga agtcgttgaa cccgtagcgc cggtgcgccg cctccgcctg gcgaacgatg
     4981 ctctccgcgg tcgtcgcctc ctcgttcctg aggcgcaacc agtcgtcgga tgcgtcgggt
     5041 tcgcttcggt acgggaggtc ggtgcgcgtg cgatcgccca cgtagaagag atatcccagc
     5101 acttcgacct ggctgcgttg ccgcccctcg cccagaagat cggctatggg cacgccgagg
     5161 aactgcccca gcaggtcgag catcgcggac tccaccgccg tcatggcgtg gatcgtggtg
     5221 cgcaagtcga acgtctgcaa tccgcgcccg ccggcatcgc gatcggcgaa acgccggcgc
     5281 atgtcctgga gcaccgcgtt gaccgaactc accgtccgcc cgaccacgag cgggcgggcg
     5341 tcctcgatgg tcgcgcggat cttctcgccg cccggcactt cgccgacacc ggtgttcccg
     5401 gcgctgtccc gaatgatgac gacattgcgg gtgaagaacg ggccgtgtgc gccgctcagg
     5461 ttgagcagca tgctgtcgtg tcccgcgacg ggaacgacgc gcaattccgt gacggcaggg
     5521 gcgccgcgtg cggggctttg caggggaggc ggggacatga tgtggcaggt gggccgcggc
     5581 gcggatttcg ttgatggtat cggtaacatc aatggcgtgt caatcgccgc gcttgcctat
     5641 gggaagttgc cgtcgcgcgt gatgacagcg gtcccatttg ccagtccggc ccccggcggc
     5701 tatcctgacc gctggactcc ctcggcccac ccatggcgcg cacgcgttcc gcacggaagc
     5761 aaccccgcaa gccccggcgg ggatccgtcc aatccacagc gcgcgctgct tcgggcaagg
     5821 cggtcacgat ccgcgacgtc gccaaggtcg ccggcgtttc gcccatcacc gtatcgcggg
     5881 cgctgagccg ccccgagctc gtcaccccgg agaccctcga gcgcatccgc gcggtggtct
     5941 cgcgcacggg ctacgtgcgc aacgtcatgg ccgggagcct gcgctcgcgc cgcagccgga
     6001 tcatcgcggc ggtctttccg caggtcgcga acaccatgtt cgtcgacacg atccaggcgc
     6061 tgatggaccg cctgcggcag gacgggtacc aggtcctgct ggggctctcg tcctaccaga
     6121 tgcgcgagga cgaactcatc gcgggcattc tcggccggca gcccgagggc atcttcctca
     6181 ccggcatctc gcacgccccg gagacgcggc gccggctcat cgcctcgcat attcccgtcg
     6241 tcgaggcgtg ggacctcacg ccgacgccca tcgacatgct cgtcggcttt tcccacgaga
     6301 aggtgggcgc ggcgatggcg cgccatctcc tcggcaaggg gcatcgcgag atcgggctgg
     6361 tctggagcac ggatatccgc gccggaatgc gcaggcaggg tttcctgtcg gtcctcgcgg
     6421 agaatggcat cacgcaggcg ccgacaagtc tcaggccggc gccggccacg ttccagcgcg
     6481 ggcgcgaagg actggccgag ctgctgggat cgggcgcgcg ggtgagcgcg gtcgcgtgcg
     6541 gctccgacac gctcgcgcaa ggcgtgatcg cggaggcgca ggatcgcggg atccggattc
     6601 cgggggacat cgcggtgatg ggtttcggca acctcgattt cgcggcacac acgacgccgg
     6661 ccctcacgac catcggcatc gacaaggcgg cgatcgggcg cctcgcggcg gaagcgctga
     6721 tcgcgagaat cgaaggccgg gaggttcgcg agaagatcgt cgacgtgggc ttcgagctga
     6781 tcgagcgcga aagcacctga ggccggccag ggcgcgcgcc gctcagtcga tcttcaccca
     6841 ggtcatctcg agatagttgt cggggcggtg gaagaccgtc acgcccttgc gcaccgccca
     6901 ggggatcact tgccggtgca gcgggatggt gtaggcgttc gtgcgcacgc gctgcagcgc
     6961 ttcctccatc agcgccgcgc gccgtgccgg gtccatctcg acctcgctcc tctcgatcag
     7021 gcggtcgagc tcggcgtccc tgaacctgcc ggaattgaag tcgcccttgc ccttgccgtc
     7081 gaaggagtgc agcaccggcc cgagaacgag gccggggtcc gtgggcgc
//
LOCUS       DASUEV010000342         7939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_322638, whole genome shotgun sequence.
ACCESSION   DASUEV010000342 DASUEV010000000
VERSION     DASUEV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322638"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(660..2564)
                     /locus_tag="VFV55_10805"
     CDS             complement(660..2564)
                     /locus_tag="VFV55_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX4944835.1"
                     /translation="MSSPAAPAPKIHVSQASAATLVLGALGVVFGDIGTSPLYALKEC
                     FSPEHGIAVSPANVMGLLSLITWSMAWVIAFKYLSVMMRADNNGEGGILALLALALRG
                     LDRETRLRRYVVMLGIFGAAMFYGDSMITPAISVLSAVEGLEIATPVFSHWVIPATIA
                     ILTALFVIQRHGTASVGALFGPVTALWFLAIGATGIWRISQNPAILAAANPAHAVSFL
                     VDNPLLGFLVLGAVFLALTGGEALYADMGHFGKRPIRIAWFGLVMPALLLNYFGQGAF
                     VLGNPEGVKNPFYLMLPEWAVLPMVALATAATVIASQAVISGAFSMTRQAIQLGYIPR
                     LEILHTSSRAIGQIYVPLVNWTLFVAVVLLVLGFRTSGNLANAYGIAVAATMVIECLL
                     AIVVADSLWKWPPVATWGIIGSMLVVDLFFLASNAAKISSGGWFPLLIGAGCFTLLVT
                     WKRGRALLTQRLSEQGIPLEPFLASLALSPPVHVPGTAIFMTGTSDTVPAALLHNLKH
                     NKVLHDRVVFLTVVTHDVPVVPIEDRIQLKRLGDGFWKVEAWYGFKEHPDIAEMLDSC
                     RLRYGLGFDPMETSFFLSRENIVAGERPGMAAWRDHLFAWMNRNATRATDFFNIPINR
                     VVELGTHVDI"
     gene            complement(2652..3272)
                     /locus_tag="VFV55_10810"
     CDS             complement(2652..3272)
                     /locus_tag="VFV55_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX4944836.1"
                     /translation="MYQGERFNSISHLLAALLALAGGVVLIVIGSRQADPWKIVSFSI
                     YAATLFFLYLTSTLYHSTRGGRAKWIYRKLDHSAIYLLIAGTYTPFTLVTLNGPWGWS
                     LFGIVWGLALLGIVQELCFAKGARWLSLAIYLLMGWVAVVAVVPLIEALSWAGFAWLI
                     SGGVIYTAGVAFYVFDERFRHFHGIWHIFVVAGSAVHYTAIVLYVA"
     gene            complement(3307..3468)
                     /locus_tag="VFV55_10815"
     CDS             complement(3307..3468)
                     /locus_tag="VFV55_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944837.1"
                     /translation="MAKPNYAFEKRKRELEQKRKKEEKLQRKMQNKQAKDGDGTESAP
                     APEADAPPA"
     gene            complement(3575..4411)
                     /locus_tag="VFV55_10820"
     CDS             complement(3575..4411)
                     /locus_tag="VFV55_10820"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4944838.1"
                     /translation="MAKVFIAGAGYVGTVAAVLLARAGHVVDIGRRTPGETPRSHAMD
                     VLRPETFPPALRDAACVVYCVSADGFTPGSYRDAYVEGLARVIEAASAGCARRLVFVS
                     STGVFGQDDGSIVDEASPAEPQGFSGRTLLEGEALLAAAPFEATAIRFSGIYGPGRDR
                     LIRMVRGRTPVPEKSRAAITNRIHRDDCARALVHLVERPAVAPLYLGSDTSPTPMGEI
                     LDWIAARLGMDPPPLDGDSAGSPQRGGNKRISSARLRGEGFEFLYPTFREGFEAILAA
                     GA"
     gene            complement(4411..5082)
                     /locus_tag="VFV55_10825"
     CDS             complement(4411..5082)
                     /locus_tag="VFV55_10825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX4944839.1"
                     /translation="MSKDTWNAVDQYLTDTLVRPDAVLEAALRESEAAGLPAIHVSPP
                     QGKLLNLLARVQGARNILEIGTLGGYSTIWLARALPPGGGRIVTLEVDATHAKVARAN
                     LARAGFANMVQVRLGRAIDTLPELEHEGAGPFDLVFIDADKPSNPEYFAWALKLSRRG
                     TVIVVDNVVRNGKVADGKSGDPAVQGVRRLNLMMASEPRVSATAIQTVGSKGYDGFAI
                     ALVVS"
     gene            complement(5119..5778)
                     /gene="can"
                     /locus_tag="VFV55_10830"
     CDS             complement(5119..5778)
                     /gene="can"
                     /locus_tag="VFV55_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX4944840.1"
                     /translation="MRSLSRLFENNKSWSERIRKHDPEFFRELSRQQSPAYLWIGCAD
                     SRVPANEIVGLLPGELFVHRNVANLVVHTDLNCLSVIQFAVDHLKVRHVIVCGHYGCS
                     GVRAALGRDRLGLVENWLRHVQDVRNKHADVISALAGAAAQADRLCELNVIEQVVNVC
                     ETSIVRDAWERGQDVSLHGWIYGIADGLLRDLGMTVSALAEVEARRAEALANLGKLRD
                     A"
     gene            complement(5789..6448)
                     /locus_tag="VFV55_10835"
     CDS             complement(5789..6448)
                     /locus_tag="VFV55_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944841.1"
                     /translation="MFIGHFGAGFGAKALVPRVSLGWLFVAAQFIDLLWPALLLAGVE
                     RVRIAPGATAVTPLVFEHYPVSHSLLAVCGWALFVGGTFFAIAKDRRGAIVLAALVVS
                     HWLLDAIVHQPDLPLYPGSATLVGLNAWSSLPLSIAIEVPIFALGAWLYARSTTPRDA
                     VGRWAFAGLVAFLLAIQAGNYFGAPPPGAEAIAWVGLAQWLLVLWGFWVDNHRSAKVR
                     A"
     gene            6550..7233
                     /locus_tag="VFV55_10840"
     CDS             6550..7233
                     /locus_tag="VFV55_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX4944842.1"
                     /translation="MATTRSIVPGIPALLAAIVSAFAVTAARGDAPMQPADLFLRVCS
                     VCHGEKGDGKSLASTALKTSPRDFTTEEARTSLSREYMIAIVRDGRPHSAMVGRTMRL
                     THAEIEGVVDFIRTAFMAPEPGTPLAQGRALYRKSCASCHGDRGQGGPARGAIPASAP
                     ISAARARAGLTSEDMVEAISRERHIPGLPVVGSGLTADEVSAIVGYIRTAFIEAAAGR
                     RAAGLPKAE"
     gene            complement(7253..7732)
                     /locus_tag="VFV55_10845"
     CDS             complement(7253..7732)
                     /locus_tag="VFV55_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX4944843.1"
                     /translation="MGSTIAFVIAASTLAIATAVTAQSRASQAPKHRTIYIAAIEPKG
                     SAHVGDEPFPAMPLPPGGGYSLKKPDEKGRWEVSTYRWDPGTIVVNQGDKVTLEIVGI
                     NGDEHPFTIEGYWISDVVRRGQITRVNFTADKAGIFKIICRKHAPAMQADLVVLESK"
     gene            complement(7745..>7939)
                     /locus_tag="VFV55_10850"
     CDS             complement(7745..>7939)
                     /locus_tag="VFV55_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HEX4944844.1"
                     /translation="VHHRDHNHDNNPPDGSNWELLCVYCHDNEHQRQLEATGGSGETA
                     AKGTTHSPFADLKAMLERKR"
BASE COUNT         1324 a         2592 c         2804 g         1219 t
ORIGIN      
        1 cggcaccgct gccttcgccc ttcttctcga tggccgtgac ggaactgatg gtcccgcagt
       61 cgcggcagac tctcgcgacg ggcgcaggct cggggctggc cgcggccgaa ggcggaacgg
      121 gaggcgggta tgccggcgcg ctgttcgcgt aactcggcgt gctcggcgca ggccttgcgt
      181 aagcgggtgc gggcggaggt gccggcttgg caacatgcgg cttcggcgcc ggcttccgcg
      241 gaggcggcag cggctggaca ggcgccggtg tctcttgcgg aacgggagcc ggttccgcgg
      301 cgacaggcgc gggaggtggt gcgggcacgg cggtgacagg cgccggagcg ggcgccggcg
      361 cgacggcaac atcgggaacc acgccgccct gttgcgcggg attggccatg ccgaccggcg
      421 tggcgggcgc tttcgcaggt tgcccgggga gcagcccgag gtaggaagcc gcgccggcgg
      481 ccgcaaagac cacgatggca atggcgccga cgacaaggcc gggatggatg cccggcttgg
      541 gcgtggcgct gctggctgcg ggagtgttca tcgatttctc ctgtttatgg ccgcgccgct
      601 cccatggcgg cgcatcccca cacgttaacg cgaatcgcct ccgattcgtt gacgaccggt
      661 cagatgtcca cgtgcgtgcc gagctccact acgcgattga tcgggatgtt gaagaagtcg
      721 gtggcgcgcg tggcgttgcg gttcatccac gcgaagaggt ggtcgcgcca ggcggccatt
      781 cccggtcgct cgccggcgac aatgttctcg cgcgacagga agaacgacgt ttccatcggg
      841 tcgaagccca gcccgtagcg cagccggcac gaatcgagca tctccgcgat gtccgggtgc
      901 tccttgaagc cgtaccacgc ctcgaccttc cagaatccgt cgccgagccg cttcaactgg
      961 atgcggtcct cgatcggcac gacgggcacg tcgtgcgtga ccacggtcag gaacaccacc
     1021 cggtcgtgca gcaccttgtt gtgcttgagg ttgtgcagga gagccgccgg aacggtgtcc
     1081 gacgtgccgg tcatgaatat cgcggtcccc ggaacgtgca cgggcgggct cagcgcaagg
     1141 cttgcgagga agggctcgag cgggatgccc tgctcggaga ggcgctgcgt gaggagggcg
     1201 cgcccgcgct tccacgtgac gaggagcgtg aagcagcccg cgccgatgag cagcgggaac
     1261 cagccgccgg aggagatctt ggctgcgttt gaggcgagga agaagaggtc gaccacgagc
     1321 atcgacccga tgattcccca cgtcgcgacg ggcggccatt tccacaggct atccgccacg
     1381 acgatcgcaa gcaggcactc gatcaccatc gtggcggcga cggcaatgcc gtatgcattg
     1441 gcgagattgc cggaggtcct gaagccgagc accagcagga cgacggcgac gaacagcgtc
     1501 cagttcacga gcgggacata gatctgcccg atggcccgcg aggacgtgtg caggatctcg
     1561 agccgcggga tgtagcccag ctggatcgcc tggcgggtca tggagaaggc cccggagatg
     1621 accgcctggg aggcgatcac cgtggccgcc gtggcgagcg cgaccatggg aagcacggcc
     1681 cactccggaa gcatcaggta gaaggggttc ttcacgcctt cggggttgcc gagcacgaac
     1741 gcgccctgcc cgaagtagtt gagcagcagt gccggcatca cgaggccgaa ccaggcgatg
     1801 cggatggggc gctttccgaa gtgccccatg tccgcgtaga gcgcctcgcc gccggtcagc
     1861 gcgaggaaca cggccccgag gaccaggaat ccgaggagcg gattgtcgac gagaaacgaa
     1921 accgcgtgcg cgggattggc cgcggcgagg atcgcgggat tctgcgaaat gcgccagatc
     1981 cccgtcgcgc cgatcgcgag aaaccacagg gcggtgaccg ggccgaagag cgcgcccacg
     2041 ctggcggtgc cgtgccgctg gatgacgaag agcgccgtga ggatggcgat cgtcgccgga
     2101 atcacccagt gcgagaagac cggcgtggca atctccaggc cttccaccgc ggagagcacc
     2161 gagatggccg gagtgatcat gctgtcgcca tagaacatcg ccgcgccgaa gatgcccagc
     2221 atgacgacgt accgccgcag gcgcgtctcg cggtccaggc cgcgaagcgc gagcgcgagg
     2281 agggcgagga tgccgccctc gccgttgttg tccgcccgca tcattaccga gaggtacttg
     2341 aaggcgatca cccacgccat cgaccaggtg atgagcgaga gcagccccat cacgttcgcg
     2401 gggctcaccg cgatgccgtg ctccgggctg aagcattcct tgagcgcata cagcgggctc
     2461 gtgccgatgt cgccgaatac gacgccaagg gccccgagca cgagcgtggc cgcggatgcc
     2521 tgggatacgt ggatcttcgg ggccggcgct gcgggggaac tcatcgatgc tcccgcgccg
     2581 gacgggatcc ggcgcgaatg ggcggggact atacgctcga atgctgcact gcacaacgcc
     2641 cggtcagggc gtcacgcgac atagagcacg atggccgtgt aatgcacggc gctgccggcc
     2701 accacgaaga tgtgccagat gccgtggaag tgcctgaacc tttcgtcgaa gacgtagaag
     2761 gcgacgccgg ccgtgtagat cacgccgccg gatatcagcc acgcgaaccc cgcccaggaa
     2821 agcgcctcga tgagcgggac caccgccacc accgccaccc agcccatgag caggtagatc
     2881 gcgagcgaaa gccagcgcgc gcccttcgcg aaacacagtt cctgcacgat gcccaggagc
     2941 gccagccccc agacgatgcc gaagagcgac catccccacg ggccgttcag ggttaccagc
     3001 gtgaacggcg tgtaggtgcc ggcgatgagc aggtagatcg cgctgtggtc gagcttgcgg
     3061 tagatccact tggcccggcc gccgcgcgtg gagtggtaaa gcgtggaggt gaggtacagg
     3121 aagaagagcg tggcggcgta gatcgagaag ctcacgatct tccacgggtc ggcctgccgc
     3181 gagccgatga cgatgagcac gaccccgccg gccagcgcga ggagcgccgc gaggaggtgg
     3241 gaaatgctgt tgaaccgctc gccttgatac atcgggggcc gaccccgaga tgaagatggc
     3301 gcgacttcag gcgggcggag cgtcggcctc gggggccggg gcggattcgg tgccgtcgcc
     3361 gtcttttgcc tgcttgttct gcatcttgcg ctggagcttt tcttccttct tgcgcttctg
     3421 ctccagctcg cgcttgcgct tctcgaatgc gtagttgggt ttggccaagt tcgtgttctc
     3481 cttccggtcg ttggacccga caattctcgc acggacggca tgcaagcgcc tgccggggtg
     3541 caagaaatca gcttcgtgcc gccccgcccg gccgctacgc cccggcggcc aggattgcct
     3601 cgaacccctc gcgaaaagtg ggatagagga actcgaaccc ctcgccacgc aggcgggcgc
     3661 tggaaatgcg cttgttgccg ccgcgctggg gcgaaccggc ggagtcgccg tccaggggag
     3721 gcgggtccat gcccagcctt gccgcgatcc agtcgaggat ctcgcccatc ggcgtggggg
     3781 atgtgtcgct gccgaggtag agcggggcga ccgccggccg ctcgacgaga tgcaccaggg
     3841 cgcgcgcgca gtcgtcgcgg tggatgcggt tggtgatggc cgcgcgcgac ttctccggga
     3901 cgggcgtgcg cccgcgcacc atgcggatga ggcggtcgcg tcccggcccg tagataccgg
     3961 aaaagcggat cgcggttgcc tcgaaaggcg cggccgccag gagtgcttcg ccctcgagca
     4021 atgtccgccc ggagaagccc tgcggttcgg cgggcgaggc ctcgtccacg atgcttccgt
     4081 cgtcctgccc gaagaccccg gtcgacgaaa cgaacaccag ccgccttgcg cagcccgccg
     4141 aggcggcttc gatgacgcgc gccagtccct cgacataggc gtcgcggtag gacccgggcg
     4201 tgaacccgtc ggcggaaacg caatacacca cgcaggccgc gtcgcgaagc gccgggggaa
     4261 acgtttcggg acgaagaaca tccatcgcgt gcgaacgggg cgtctcgccc ggcgtgcgcc
     4321 ggccgatgtc gaccacatgg cccgcgcgtg cgagcagcac cgcggcgacc gtgcccacgt
     4381 agccggcccc ggcgatgaag accttcgcca tcaggagacc acgagcgcga tggcgaagcc
     4441 gtcgtagccc ttgctgccga cggtctggat ggcggtcgcg gaaacgcgcg gctcggaagc
     4501 catcatcagg ttcagccggc gcacgccctg cacggccgga tcgccgctct tgccgtcggc
     4561 gaccttgccg ttgcgcacca cgttgtccac gacgatcacg gtgccgcgcc gcgaaagctt
     4621 cagcgcccag gcgaagtatt cggggttcga cggcttgtcg gcgtcgatga agacgaggtc
     4681 gaaggggccc gcgccctcgt gctcgagttc gggaagcgtg tcgatggcgc ggcccaggcg
     4741 cacctggacc atgttcgcga acccggcgcg cgcgagattg gcgcgcgcga ccttggcgtg
     4801 cgtcgcgtcc acctcgagcg tgacgattcg tcctccccct ggtggcagcg cgcgggcgag
     4861 ccagatggtg ctgtagccgc ccagcgtgcc gatctcgagg atgttcctcg cgccctggac
     4921 gcgcgccaga aggttgagaa gcttgccctg cggcggcgac acgtggatcg cgggaagccc
     4981 ggccgcctcg ctctcgcgca gcgcggcttc gaggaccgcg tccggcctga ccagcgtgtc
     5041 ggtgaggtat tgatcgactg cgttccaggt gtccttgctc atgccgccaa gactatcacc
     5101 tgtgcgtggc ctcgcgcgtc acgcgtcacg aagcttgccc aggttcgcca gcgcctccgc
     5161 gcgccgcgct tccacctcgg cgagagcgct caccgtcatg ccgaggtctc gcagcagccc
     5221 gtccgcgatc ccgtagatcc agccgtgcag cgagacatcc tgcccgcgct cccaggcgtc
     5281 gcgcacgatg gaggtctcgc acacgttgac cacttgctcg atgacgttga gctcgcacag
     5341 ccggtcggct tgcgcggccg ctccggccag tgcggaaatc acgtccgcgt gcttgttgcg
     5401 cacgtcctgc acgtgtcgca gccagttctc cacgagcccc agccggtcgc gcccgagggc
     5461 cgcgcggacg ccgctgcaac cgtagtgccc gcagacgatg acgtggcgca ccttgaggtg
     5521 atccaccgcg aactggatga ccgaaaggca gttgaggtcc gtgtggacga ccaggttggc
     5581 cacgttgcgg tgcacgaaga gctcgcccgg caggagcccg acgatctcgt tggcgggcac
     5641 gcggctgtcc gcgcatccga tccagagata cgcaggcgac tgctgccgcg acagttccct
     5701 gaagaattcc gggtcgtgct tgcggatgcg ctcggaccac gacttgttgt tctcgaacag
     5761 gcgagaaagg gatcgcatgg cgtggcgttc aggcgcggac tttcgcgctg cggtgattgt
     5821 cgacccagaa accccacagc accagcagcc actgcgccag ccccacccac gcgatggctt
     5881 ccgcgcccgg cggcggcgcg ccgaagtagt tgccggcctg gatggcgagc aggaaggcga
     5941 cgagccccgc gaacgcccag cgcccgacgg catcgcgcgg cgtggtgctg cgcgcgtaca
     6001 gccacgcgcc gagggcgaag atcggcacct cgatggcgat cgagagggga agcgagctcc
     6061 acgcgttcaa cccgacgagc gtcgcggagc cggggtagag cggcaagtcc ggctggtgga
     6121 cgatggcatc gagcagccag tggctcacga ccagtgccgc cagcacgatc gcgccgcggc
     6181 ggtccttcgc gatcgcgaag aaggtgccgc ccacgaaaag cgcccacccg cacacggcca
     6241 gcaggctgtg cgacaccgga taatgttcga agacgagcgg ggtgacggcg gtcgcgcccg
     6301 gcgcgatgcg cacgcgctcg actccggcga gcagcagcgc gggccagagt agatcgatga
     6361 actgcgcggc cacgaagagc cagccgagcg aaacccgggg gaccagcgcc ttggcgccga
     6421 agcccgcgcc gaagtgaccg atgaacatcg cgatggcgtg tgggatcgaa ggcggctcat
     6481 tctgtccgta agggtggaac cggtcaatgc accgctgccg gccgcaagga agtcgagtaa
     6541 agttgccgcg tggcgaccac gcgctcgatc gttcccggca tacccgcact cctcgcggcc
     6601 attgtttcgg ccttcgcggt gaccgccgcg cgcggtgacg cgccgatgca gcctgcggac
     6661 ctgttcctgc gcgtctgttc cgtgtgccac ggggaaaagg gcgacggcaa gagcctcgcg
     6721 agcacagctc tcaagacctc gccgagagac ttcaccacgg aggaggcgcg caccagtctc
     6781 tcgcgcgaat acatgatcgc gatcgtacgc gatggccggc cgcactccgc gatggtcggg
     6841 cgcacgatgc gacttaccca cgccgagatc gaaggcgtcg tcgacttcat ccgtacggca
     6901 ttcatggcgc ccgagcccgg cacgcccctc gcccagggcc gcgccctcta tcggaagtcc
     6961 tgcgcgtcgt gccatggcga tcgtgggcag ggcggcccgg cgcgtggcgc gattccggct
     7021 tcggcgccga tttccgctgc gcgtgccagg gccggcctga catcggaaga catggtcgag
     7081 gccatctcgc gcgaacggca catcccgggt ttgccggtcg tcgggtcggg gctgacggcg
     7141 gacgaagtca gtgccatcgt aggttacatt cgcaccgcgt tcatcgaggc cgccgcggga
     7201 aggcgcgcgg ccggcctgcc gaaggccgag tgacggctgc gcaacggctg aattacttcg
     7261 attccagcac caccagatcg gcctgcatgg cgggcgcgtg cttccggcaa atgatcttga
     7321 agatgcccgc cttgtccgcg gtgaagttca cgcgggtgat ctgcccgcgc cgcacgacat
     7381 cggaaatcca gtagccctcg atcgtgaacg gatgttcgtc gccgttgatg cccacgattt
     7441 cgagcgtgac cttgtcgccc tgattgacca cgatggtccc gggatcccag cgataagtgg
     7501 agacctccca gcggcctttc tcgtcgggct tcttcaggct gtagccgcca ccggggggca
     7561 agggcattgc ggggaagggc tcatccccga catgggcgct gcccttgggc tcgatggcag
     7621 cgatgtagat ggtgcggtgc ttcggcgcct gcgaggccct ggactgcgcg gtgacagcgg
     7681 tcgcgatcgc gagggtgctg gcggcgatga cgaaagcgat ggtgcttccc atggcgtccc
     7741 gggcctatcg ttttcgctcg agcatcgcct tcaggtcggc gaagggcgag tgcgtggtgc
     7801 ccttggcggc ggtttcgccg ctgcctccgg tcgcttcgag ctgccgctgg tgctcgttgt
     7861 cgtggcagta gacgcacagg agttcccagt tgctcccgtc cggcgggttg ttgtcgtggt
     7921 tgtggtcgcg atgatggac
//
LOCUS       DASUEV010000343         4392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_110127, whole genome shotgun sequence.
ACCESSION   DASUEV010000343 DASUEV010000000
VERSION     DASUEV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110127"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFV55_10855"
     CDS             <1..546
                     /locus_tag="VFV55_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4944845.1"
                     /translation="LLVASIECDDPVIFLEPKRLYNGPFDGHHDRPMVPWSGHALAEV
                     PEGHYTVPLESAAVRRPGNSVTVLTYGTMVWVSEAAARETGIDAEIIDLRSLWPLDLD
                     TIVESVTRTGRCVIVHEATRTSGFGAELAALVQEHCFFHLEAPIERVTGWDTPYPHAQ
                     EWAYFPGPARIGAALRRAMEA"
     gene            549..1742
                     /locus_tag="VFV55_10860"
     CDS             549..1742
                     /locus_tag="VFV55_10860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX4944846.1"
                     /translation="MVDGRADGNRTVHVIKVPDIGEGIAEVELVAWHVQPGDAVVAEQ
                     AIADVMTDKATVEIPSPVSGRVLALGGKAGETLAVGSELLRIEEDGAGGVETAVARAP
                     DATAPEARPLASPSVRRHARELGVDLKNAHGSGPDGRILHEDLAKTSARGGSLPAGGQ
                     RYAERSGEQAVPVIGLRRQIAQRMQEATRRIPHFTYVEEIDVTELEALRARLNEERGA
                     ARGRLTLLPFLMRAIVLAVPGFPQMNARYDDENGVVTRYAAVHIGIATQTEGGLMVPV
                     VRHAEARDLWSSAAEVARLAEAARKGKAAREELSGSTITITSLGPLGGIVVTPIINAP
                     EVAIVGINRIVERPVMRSGAIVPRRIMNLSSSFDHRVVDGQHAAEFVQALRSRLECPA
                     LLFVD"
     gene            complement(1739..4018)
                     /locus_tag="VFV55_10865"
     CDS             complement(1739..4018)
                     /locus_tag="VFV55_10865"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX4944847.1"
                     /translation="MDEQLRIDALEYHRLPRPGKISVTPTKALINQRDLALAYSPGVA
                     AACDAIAADPNEAYSLTSRGNLVAVITNGTAVLGLGNIGPLAGKPVMEGKACLFKKFS
                     GIDVFDIEIAEKDPEELVKTIARLEPTFGGINLEDIKAPDCFYVERKLRERMKIPVFH
                     DDQHGTAIVASAAILNAMKVVGKDFAKVKLVTSGAGVAAIACLDLLVGLGIRRENVMM
                     CDSKGVIFVGRETPMEENKARYARDTKARKLADAIAGADIFLGLSGPKVLTPEMVKTM
                     APDPFIMALANPEPEIVPELASAVRPDAIIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATSITEEMKLACVRAIAELAHAEQSDIVAQAYAGESLNFGREYLIPKPFDPRLVVTVP
                     MAVARAAMESGVATRPIADFDAYRERLSQFVYHSGLIMKPVFAGAKRAPMRVAYAEGE
                     EERVLRAVQVVVDEGMAKPILIGRPEVIAARIEKFGLRLTAGKDFELVNINSDPRYKS
                     YWQTYYQLMGRKGISPEDAKEAVLRKPSLIGALLLQIGDCDALLCGTVGKYHAHLQHV
                     ANVIGRRPDAPVFAAMNGLLLPNRTVFICDTYVNRNPSAEELVEIARLAADEVRRFGL
                     TPKVALLSHSNFGSDDSKSARKMREARELLAKRVPGLEVDGEMHGDAALSEAIRSRVH
                     PDSALKGGANLLVMPNLDAANISFNLLKITGGDGVTVGPILLGARKPVLIATPSATVR
                     RLVNLTALAVVDAGAGPAD"
     gene            4177..>4392
                     /locus_tag="VFV55_10870"
     CDS             4177..>4392
                     /locus_tag="VFV55_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944848.1"
                     /translation="MKILCMHGVGHKDAEDPNSTDSNWRDAWQQAVTRGLRQWNASLE
                     PRFRFPVYDPLFERAPLKAGTVMEALAR"
BASE COUNT          682 a         1502 c         1501 g          707 t
ORIGIN      
        1 ctgctcgtcg cctcgatcga gtgcgacgac ccggtcatct tcctcgagcc caagcgcctc
       61 tacaacggtc ccttcgacgg ccaccacgac cggcccatgg tgccgtggtc cggacacgcg
      121 ctcgccgaag ttcccgaggg ccattacacg gtgccgctgg agtcggcggc cgttcgccgc
      181 cccggcaatt cggtcaccgt gctgacctac ggcacgatgg tgtgggtgtc cgaggcggcg
      241 gcacgcgaaa ccgggatcga cgccgagatc atcgacctgc gcagcctctg gccgctggac
      301 ctggacacca tcgtcgaatc ggtcacccgg accggccgct gcgtgatcgt ccacgaggcc
      361 acgcgcacca gcggcttcgg cgcggaactg gcggcgctgg tgcaggaaca ttgcttcttt
      421 cacctcgagg cgcccatcga gcgcgtgacc ggatgggaca cgccctaccc gcacgcgcag
      481 gagtgggcct actttcccgg cccggcccgc atcggggccg cgctgcggcg cgcaatggag
      541 gcgtgaccat ggtcgatgga cgagccgacg gcaacaggac tgttcatgtg atcaaggttc
      601 cggacatcgg cgagggcatc gccgaggtcg agctggtggc gtggcacgtc cagcccggcg
      661 atgcggtcgt cgcggaacag gcgatcgccg acgtgatgac ggacaaggcg accgtcgaga
      721 tcccgtcgcc cgtttcggga cgcgtgctgg cgctgggcgg caaggccggc gagacgctcg
      781 cggtgggcag cgagttgctg cggatcgaag aggatggagc gggtggagtc gaaaccgcgg
      841 tggcccgcgc accggacgca accgcgccgg aagccaggcc cctcgcctcg ccgtcggtgc
      901 gccgccacgc gcgcgagctg ggggtcgatc tgaagaacgc gcacgggagc ggtcccgacg
      961 ggcggatcct gcacgaggat ctcgcgaaga cttccgcgcg cggtggatcg ttgccggcag
     1021 gcggccagcg atacgccgag cgcagcggcg agcaggccgt gcccgtgatc gggctgcgcc
     1081 gccagatcgc gcagcgaatg caggaggcca cgcgccgcat cccgcatttc acttacgtcg
     1141 aggaaatcga cgtcaccgag ctggaggcgc ttcgcgcccg gttgaacgag gagcggggcg
     1201 ccgcgcgcgg gcggctcacg ctgctcccgt ttctcatgcg cgccatcgtg ctcgccgtgc
     1261 ccggctttcc ccagatgaac gcgcgctacg acgacgagaa cggtgtcgtg acccgctacg
     1321 cggcggtgca tatcggcatc gccacgcaga ccgaaggcgg gctgatggtc cccgtggtgc
     1381 gccatgccga ggcgcgcgac ctgtggtcga gcgcggccga ggtcgcgcgc ctggccgaag
     1441 ccgcgcgcaa gggcaaggcc gcgcgcgagg agctttccgg ctcgacgatc accatcacca
     1501 gcctgggccc gctgggcggc atcgtcgtca cgcccatcat caacgcaccg gaagtcgcca
     1561 tcgtcggcat caaccgcatc gtcgagcggc cggtcatgcg ttccggcgcg atcgtgccgc
     1621 ggcggatcat gaacctgtcg tcgtcgttcg accaccgcgt cgtggacggc cagcacgcgg
     1681 ccgagttcgt gcaggcgttg cgcagccgcc tcgagtgccc cgcgctgctg ttcgtggact
     1741 agtcggccgg ccccgccccc gcgtccacga ccgcgagcgc cgtcaggttg acgagtcgcc
     1801 gcacggtggc cgatggcgtc gcgatcagca ccggcttgcg cgcgcccagg aggatcgggc
     1861 cgaccgtcac gccgtcgccg ccggtgatct tgagcaggtt gaaggagatg ttggcggcat
     1921 cgaggttcgg catgaccagc aggttcgccc cgcccttcag ggccgagtcc ggatgcaccc
     1981 ggctgcgtat ggcttcggac agcgcggcat cgccatgcat ctcgccgtcg acttccaggc
     2041 cgggaacgcg ctttgccagc agttcgcgcg cctcgcgcat cttgcgcgcc gacttcgaat
     2101 cgtcgctgcc gaaattggag tgcgacagca gcgccacctt gggcgtcagg ccgaaccgcc
     2161 gcacctcgtc cgcggcaagc cgcgcgatct cgaccagctc ctcggccgaa gggttgcggt
     2221 tcacgtaggt gtcgcagatg aagaccgtcc ggttgggcag gagcagcccg ttcatcgcgg
     2281 cgaacaccgg ggcatccggc ctgcgcccga tgacgttggc gacgtgctgc aggtgagcgt
     2341 ggtacttgcc cacggtgccg cacagcagcg cgtcgcaatc gccgatctgc agcagcagcg
     2401 cgccgatgag cgaaggcttg cgcagcaccg cctccttcgc gtcctcgggc gagatgccct
     2461 tgcgacccat gagctggtag taggtctgcc agtacgactt gtagcgcgga tcgctgttga
     2521 tgttgacgag ttcgaaatcc ttgccggccg tgaggcgcaa cccgaacttc tcgatccgcg
     2581 cggcgatgac ctcggggcgg ccgatcagga tgggcttcgc catcccctcg tcgacgacga
     2641 cctgcaccgc ccggagcacg cgctcctcct cgccttccgc gtaagccacg cgcatcggcg
     2701 cccgcttggc cccggcgaac accggcttca tgatgagccc ggagtgatag acgaactgcg
     2761 acagccgttc gcgataggcg tcgaaatcgg cgatcggacg cgtggccacg ccgctttcca
     2821 tggcggcccg cgcgactgcc atcggcacgg tcacgacgag gcgcggatcg aagggcttgg
     2881 ggatcaggta ctcgcggccg aaattcaggc tctcgccggc ataggcctgg gccacgatgt
     2941 ccgactgttc ggcatgcgcg agctcggcga tggcgcgcac gcaggcgagc ttcatctcct
     3001 ccgtgatcga ggtcgcgccg acgtcgagcg ccccgcggaa gatgaacggg aagcagagca
     3061 cgttgttcac ctggttcggg taatccgacc ggcccgtggc gatgatggcg tcgggccgca
     3121 cggcgctcgc gagctccggc acgatctccg gctcgggatt ggcgagcgcc atgatgaacg
     3181 gatcgggcgc catggtcttg accatctccg gcgtgagcac cttcggaccg gaaagcccca
     3241 ggaagatgtc cgcgccggcg atggcgtccg cgagcttgcg cgccttcgtg tcgcgggcgt
     3301 agcgcgcctt gttctcctcc atcggcgtct cgcggccgac gaagatcacg cccttgctgt
     3361 cgcacatcat gacgttctcg cgcctgatcc ccaggccgac cagcaggtcg aggcacgcga
     3421 tcgcggccac tcccgccccc gacgtcacga gcttcacctt cgcgaagtcc ttgcccacga
     3481 ccttcatcgc gttgaggatc gcggcggacg cgacgatcgc cgtgccgtgc tggtcgtcgt
     3541 ggaacaccgg gatcttcatc cgctcgcgca acttgcgctc gacgtagaag cagtcgggcg
     3601 ccttgatgtc ctcgagattg atgccgccga aggtcggctc cagccgcgcg atcgtcttca
     3661 cgagctcttc cggatccttc tcggcgatct cgatgtcgaa cacgtcgatt ccgctgaact
     3721 tcttgaacag gcacgccttg ccctccatca ccggcttgcc ggccagcggc ccgatgttgc
     3781 ccaggcccag gaccgccgtg ccgttcgtga tgaccgcgac gagattgccg cgggaggtga
     3841 gcgaatacgc ttcgttcgga tctgccgcga tggcatcgca agcggccgca acccccggcg
     3901 aataggcgag cgcgaggtca cgctggttga tgagcgcctt ggtcggcgtc accgagatct
     3961 tgcccggcct cggcaggcgg tggtactcca gtgcgtcgat gcgaagttgt tcgtccatgc
     4021 ggggctcctg tgcgattcga cggtgcagtc acaggacgat tctactcacg tggcgcgccg
     4081 gcaggtgcct gaaattcgcg ttcgggcacg cagggccgcc ggggtttaca ttacgggccg
     4141 ttggttttcc cggcgcgcac cgaaacggag gcgacgatga agattctctg catgcatggc
     4201 gtaggccaca aggacgccga ggacccgaac tcgacggatt cgaactggcg cgatgcgtgg
     4261 cagcaggccg tcacgcgcgg ccttcgccag tggaacgcct cgctcgagcc gcgatttcgc
     4321 ttccccgtct acgacccgct cttcgagcgc gccccgctca aggcaggcac ggtgatggag
     4381 gcgctcgcga gg
//
LOCUS       DASUEV010000344         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1249472, whole genome shotgun sequence.
ACCESSION   DASUEV010000344 DASUEV010000000
VERSION     DASUEV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249472"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VFV55_10875"
     CDS             <1..1386
                     /locus_tag="VFV55_10875"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX4944849.1"
                     /translation="MNFTAVSIAMAPEHLLLAGIVALLVLDIVSERSRAALPLAVLFV
                     VASTVAALILGFNGWTGAPFPGQFVLTPPASFAKALVLALAVPVLMFSKDDFGEGAFY
                     PLVLSSLYGVCLLASAESFLTLFLGIELMSIPVYVLVLIGFRRDESAEAALKYLVLSG
                     TASASFLMGASLMYGSTGSLGLPSFGAALSSPDLLARTAVVMVVAAFFLKAAIVPFHG
                     WAPDAYEAASIPSTAYMAVLVKAGVLIAAARVFGTAPVTEPLAGLLAVLPLASMVWGN
                     LTAMRQPTLRRMIAYSSIAHAGYLFFAFLGDGPGRFQAVLFYLATYGVMNLLAFAALP
                     SNDNDLERDRLENLKGLFRTHPFAALLIAMSMLSLAGIPPFPGFVGKFLIFKNVMAAG
                     YTTWAVLGLVGSYIGIYFYLRVIQFMFMSPGTAAAGHAPSRGIAMGASLLCLAGSILI
                     AVLPGIFLARF"
     gene            1448..1523
                     /locus_tag="VFV55_10880"
     tRNA            1448..1523
                     /locus_tag="VFV55_10880"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1481..1483,aa:Phe,seq:gaa)
     gene            complement(1696..>2888)
                     /locus_tag="VFV55_10885"
     CDS             complement(1696..>2888)
                     /locus_tag="VFV55_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944850.1"
                     /translation="ATATADIQFRPQDVGASGSIYSFAVAPSALVKGGQDAKAVAIGS
                     AWSAHKADPPTCVLAQLSSTGQLVAVTYAQLQAYSSGTLSAQGASVSILNNTSTPAVA
                     GATFYVGYGASSAAMIDEGVYRNAITVPGASVCPMLSSQTALWWNRSESGWGVNFSHQ
                     GNILFATLFTYDANRAPLWLFMSNGALQSDGATFTGELYRATGPAFNANPFTPIGPAN
                     LTPVGTMSASFTGVNSGTLRYTVNGVEVTKAIERQVYGSRAASCLPTLGSRAAATNYQ
                     DLWWNSDESGWGINVTHQDNILFATLFTYDATGRDLWLVMSSGDRQSDGSYLGDLYRA
                     SGPPFNANPFTPITIADLANVGTMRLRFSDGNNGTLTYTYNGAPVTKAITRQLFSSPV
                     PDCH"
BASE COUNT          403 a          950 c          996 g          539 t
ORIGIN      
        1 atgaacttca cggccgtatc gatcgccatg gccccggagc acctgctgct cgcgggcatc
       61 gtggcgctgc tcgttctcga catcgtctca gaacgctcgc gcgcggcgct gccgcttgcg
      121 gtcctgttcg tggtcgcgtc gacggtcgcg gcattgatcc tcgggttcaa cggctggacg
      181 ggcgcgccgt tccccggcca gttcgtcctc acgccgccgg cgtcgttcgc gaaggcgctc
      241 gtgctggcgc tcgcggtgcc ggtgctcatg ttctcgaagg acgacttcgg cgagggcgcc
      301 ttctatccgc tcgtgctctc ctcgctctac ggcgtgtgcc tgctcgcctc ggcggaaagc
      361 ttcctaacgc tctttctcgg catcgagctg atgtcgatcc cggtgtacgt gctggtgctg
      421 atcggcttcc gccgcgacga gagcgcggag gcggccctca agtacctggt actgtccgga
      481 acggcgagcg cgtccttcct gatgggcgct tcgctcatgt atggttccac cggctcgctc
      541 gggctcccga gcttcggggc cgcgctttcc tcgccggacc tgctggcccg cacggcggtg
      601 gtgatggtcg tggccgcgtt cttcctcaag gccgccatcg tcccgttcca tggctgggcg
      661 ccggacgcct acgaggccgc gagcatcccg tcgaccgcgt acatggcggt cctggtgaag
      721 gccggcgtgc tcatcgccgc ggcgcgcgtc ttcggcacgg cgccggtgac cgagcccctc
      781 gccgggctgc tggccgtcct gccgctcgcc tcgatggtct ggggcaacct caccgcgatg
      841 cgccagccga ccctgcgccg catgatcgcg tactcgtcga tcgcgcacgc gggctatctc
      901 ttcttcgcct tcctgggcga cggtccgggg cgcttccagg cggtgctctt ctatctcgcg
      961 acctacggcg tcatgaacct gctcgccttc gccgcgctgc cctcgaacga caacgatctc
     1021 gagcgcgaca ggctcgagaa cctgaagggc ctgttccgca cgcacccgtt cgccgcactg
     1081 ctcatcgcca tgtcgatgct ttcgctggcc ggcatcccgc cgttcccggg attcgtgggc
     1141 aagttcctga tcttcaagaa cgtgatggcc gcgggctaca cgacctgggc cgtgctcggc
     1201 ctcgtgggca gctacatcgg catctacttc tacctgcgcg tgatccagtt catgttcatg
     1261 agccccggca ccgccgccgc gggccatgcg ccctcgcgcg gaatcgccat gggcgcgagc
     1321 ctcctgtgcc tcgcgggctc catcctcatc gccgtgcttc cggggatctt cctcgcccgg
     1381 ttctaggagg cgggtttccc cgggttggct tcggccgctc cgaaaagcta taatttgcgg
     1441 ctttcccggc ctggtagctc agttggtaga gcagaggact gaaaatcctt gtgtcggtgg
     1501 ttcaattccg ccccaggcca ccaattcccc gtcttgatgc cgtccaccaa gatcctgaag
     1561 cccgccaagt gcgggctttg ccattcttgc gggagtgtga aatgcccctt gccaatgccg
     1621 ctatcgagag cgccaagcct ggcggagcag gagaaacgcc caccgcaagg gcaggcgctt
     1681 cccgggctgc ccggcctagt ggcaatccgg cacgggcgag gaaaataact gccgggtgat
     1741 ggccttggtc acgggtgcgc cgttgtaggt ataggtgagc gtgccgttgt tgccgtcgct
     1801 gaagcgcagg cgcatcgtgc ccacgttggc gaggtccgca atcgtgatcg gcgtgaacgg
     1861 attggcgttg aacggcggac ccgaggcgcg gtaaaggtcg ccgagatagg agccgtcgga
     1921 ctgccggtcg ccactcgaca tgacgagcca caggtcgcgg ccggtggcgt cgtacgtgaa
     1981 gagcgtggcg aagaggatgt tgtcctggtg cgtgacattg attccccagc ccgactcgtc
     2041 ggagttccac cacaggtcct ggtagttggt ggccgcggcg cggctgccca gcgtgggcag
     2101 gcaactggcc gcgcgcgagc cgtagacctg gcgctcgatc gccttggtga cctccacgcc
     2161 gttgacggtg tagcgcaagg tcccggagtt cacgccggtg aaggaagccg acatggtccc
     2221 cacgggcgtg aggttcgccg ggccgatggg cgtgaaggga ttggcgttga aggccgggcc
     2281 ggtggcgcgg tagagctcgc cggtgaaggt ggcgccgtcg gactgcaggg ccccgttgga
     2341 catgaacagc cacagcgggg cgcggttcgc gtcgtaggtg aagagcgtgg cgaagagaat
     2401 gttgccctgg tggctgaagt tcacgcccca gcccgactcc gagcgattcc accacagcgc
     2461 ggtctgcgag gacagcatcg ggcagacgct ggcgccgggc acggtgatgg cgttgcggta
     2521 gacgccttcg tcgatcatcg ccgcgctgct cgctccgtag cccacgtaga aggtggcgcc
     2581 ggccaccgcc ggggtggagg tgttgttcag gatcgagacc gaggcgccct gcgccgagag
     2641 cgtgcccgag gagtaggcct ggagctgggc gtaggtgacc gcgacgagtt gaccggtgga
     2701 ggagagctgc gccagcacgc aggtcggcgg atcggccttg tgcgcggacc aggccgagcc
     2761 gatggctacg gccttcgcgt cctggccgcc cttgaccaat gccgatggcg ccacggcaaa
     2821 cgagtagatg ctgccggagg cgcccacgtc ctgcggccgg aactggatgt cggccgtggc
     2881 cgtggcgg
//
LOCUS       DASUEV010000345         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_447709, whole genome shotgun sequence.
ACCESSION   DASUEV010000345 DASUEV010000000
VERSION     DASUEV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447709"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="hemH"
                     /locus_tag="VFV55_10890"
     CDS             <1..522
                     /gene="hemH"
                     /locus_tag="VFV55_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX4944851.1"
                     /translation="YWMKHGRPDRLVMSFHGLPRFTLDKGDPYHCQCRKSARLIATEL
                     GWNDERTIVTFQSRFGRAEWLKPYTAQTLAELGAKAVGRVDVICPGFAADCLETLEEI
                     AMEGRDTFKAAGGKDYHYIPTTNEAPAFMAALTQITLENLHGWASREWDAQAAQTEAR
                     ASAARARALGAKN"
     gene            537..887
                     /locus_tag="VFV55_10895"
     CDS             537..887
                     /locus_tag="VFV55_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX4944852.1"
                     /translation="MGSLLLRWLLNALALLAVAYLYPGVQVESFFSAVVAALALGLAN
                     ALIRPILVVLTLPVTILTLGLFLFVINAALFWLVAQIIQGFAVQGFLAALVGSILYSL
                     ITLAVSWLVAGSGK"
     gene            1001..1510
                     /gene="grpE"
                     /locus_tag="VFV55_10900"
     CDS             1001..1510
                     /gene="grpE"
                     /locus_tag="VFV55_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX4944853.1"
                     /translation="MAPEARIADLEARLAAAEAEVLRLNDDFLRARAEVENMRRRAAE
                     DVARAHKFGLESFASNLLAVKDSLEAALAVESPTTESLHQGVEITNRQLSAAFEKASL
                     TQIAPLGEKFDPHRHQAIAQVESGEEPNTVVTVVQKGYLLHDRVIRPALVMVARDRSA
                     PAPEAPPSA"
     gene            1572..>2847
                     /gene="dnaK"
                     /locus_tag="VFV55_10905"
     CDS             1572..>2847
                     /gene="dnaK"
                     /locus_tag="VFV55_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX4944854.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGKPKVIENSEGARTTPSIVAYAE
                     NDEILVGAPAKRQAITNAKNTLFAVKRLIGRRFEEKEVQKDIGLMPYKILRAENADAW
                     VEIRGRGIAPPQVSAEVLRKMKKTAEDYLGETVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKKEGDRKIAVYDLGGGTFDISIIDIAEVEGEHQFEV
                     LSTNGDTFLGGEDFDQRLIDYIVEEFRKQSGVDLSKDPIALQRVKMAAERAKIELSSS
                     QQTEVNEPYIAMANGAPSHLTMKVTRAKFESLVEDLIARTIEPCRIAIKDAGVKVSDI
                     GDVILVGGMTRMPKVQDKVKEFFGKEPRKDVNPDEAVAIGAAIQGGVLQGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIKKNTTIPTKAQQV"
BASE COUNT          545 a          954 c          925 g          423 t
ORIGIN      
        1 tactggatga agcacgggcg ccccgaccgc ctggtgatga gcttccacgg gctgccgcgc
       61 ttcacgctcg acaagggcga tccgtaccac tgccagtgcc gcaagagcgc gcgcctcatc
      121 gccaccgagc tcggctggaa cgacgaacgc acgatcgtca ccttccagtc gcggttcggg
      181 cgcgccgaat ggctcaagcc gtataccgcc cagacgctcg cggagctggg cgcgaaggca
      241 gtggggcgcg tcgacgtgat ctgtccgggg ttcgccgccg attgcctcga gacgctcgag
      301 gaaatcgcga tggaaggccg cgacaccttc aaggccgccg gcggcaagga ctaccactac
      361 attcccacga cgaacgaggc gcccgcgttc atggccgcac tgacccagat caccctcgag
      421 aacctgcacg gctgggcgag ccgtgagtgg gacgcgcaag cggcgcagac cgaggcgcgc
      481 gcaagcgcgg cccgggcccg cgcactgggc gcgaaaaact agcgaaaggt accgatatgg
      541 gctcgttgct gctgcgatgg ctgctgaatg cgctggcgct cctcgccgtc gcctacctct
      601 acccgggcgt gcaggtggag agtttctttt ccgccgtggt ggccgccctc gcgctgggcc
      661 tggccaacgc cctgatcagg ccgatcctgg tcgtgctcac gctgcccgtg acgatcctga
      721 cgctcggcct cttcctgttc gtgatcaacg ccgccctctt ctggctcgtg gcgcagatca
      781 tccagggctt cgcggtgcag ggcttccttg ccgcgctcgt gggctccatc ctctacagcc
      841 tgatcaccct cgcggtgagc tggctcgtcg cgggatcggg taaatgaccc cttgaaattc
      901 cgcggcgcgc ccccaagtgc gcttcaacgg tttgtttttc agggagaaag tcgtgtcgga
      961 ccccattgcc cccgcgccgg aatcggcgct ggatagcccc ttggcgccag aagcacgcat
     1021 cgcggacctc gaagcgaggc tcgcggcggc cgaggccgaa gtgctgcgcc tgaatgacga
     1081 tttcctccgc gcccgggcgg aagtcgagaa catgcgccgc cgcgccgccg aggatgtcgc
     1141 ccgggcgcac aagttcgggc tcgaatcgtt cgcctcgaac ctgctggcgg tcaaggatag
     1201 cctcgaggcc gcgctcgccg tcgaatcgcc gacgaccgaa tcgctgcacc agggcgtgga
     1261 gatcaccaat cgccagctct ccgccgcctt cgagaaggca tcgctcacgc agatcgctcc
     1321 cctcggcgag aagttcgatc cccaccgcca ccaggccatc gcccaggtcg aatccggcga
     1381 ggagcccaat accgtcgtga ccgtggtgca gaagggctac ctgctgcacg accgggtgat
     1441 ccggcctgcg ctcgtgatgg tggcccgcga ccgatccgcg ccggcaccgg aagcgccgcc
     1501 atcagcttga aaaaccggca aataacccca ttttccgagc aggtcaagga ccaggactaa
     1561 ggacagcgaa catggcaaaa atcatcggca tcgacctcgg cacgacgaac tcctgcgtgg
     1621 cgatcatgga gggcggcaag cccaaggtca tcgagaacag cgagggcgcg cgcaccaccc
     1681 cctcgatcgt cgcctacgcg gaaaacgacg agatcctcgt cggcgcgccg gccaagcggc
     1741 aggcgatcac caacgcgaag aacacccttt tcgcggtgaa gcgcctcatc ggccgccgct
     1801 tcgaggaaaa ggaagtccag aaggacatcg ggctgatgcc ctacaagatc ctgcgcgccg
     1861 aaaatgccga cgcgtgggtg gagatccgcg gccgcggcat cgcgccgccg caggtttccg
     1921 ccgaggtgct gcgcaagatg aagaagacgg ccgaggacta cctcggcgag acggtcaccg
     1981 aggcggtgat cacggtgccc gcctatttca acgactccca gcgccaggcc accaaggacg
     2041 ccgggcgaat cgcgggcctc gacgtgaagc gcatcatcaa cgagcccacc gcggccgcgc
     2101 tcgccttcgg cctcgacaag aaggaaggtg accgcaagat cgcggtctac gacctgggcg
     2161 gcggaacctt cgacatctcc atcatcgata tcgcggaagt cgagggcgag caccagttcg
     2221 aggtgctttc cacgaacggc gacacgttcc tgggcggcga ggacttcgac cagcgactca
     2281 tcgactacat cgtcgaggag ttccgcaagc agtcgggcgt ggacctctcg aaggacccga
     2341 tcgcgctgca gcgcgtgaag atggccgcgg agcgcgccaa gatcgagctg tcctcctcgc
     2401 agcagaccga ggtgaacgag ccttacatcg cgatggccaa cggcgcgccg tcccacctga
     2461 cgatgaaggt cacgcgcgcc aagttcgagt cgctggtgga agacctgatc gcgaggacca
     2521 tcgagccctg ccgcatcgcg atcaaggacg cgggggtgaa ggtctccgac atcggcgacg
     2581 tgatcctggt gggcggcatg acgcgcatgc cgaaggtgca ggacaaggtg aaggaattct
     2641 tcggcaagga gccgcgcaag gacgtgaacc cggacgaggc cgtggccatc ggcgccgcga
     2701 tccagggcgg cgtgctgcag ggcgaggtca aggacgtgct gctgctcgac gtcacgccgc
     2761 tttcgctcgg catcgagacg ctcggcggcg tgatgaccaa gctgatcaag aagaacacga
     2821 cgatcccgac caaggcccag caggtgt
//
LOCUS       DASUEV010000346        10062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1250248, whole genome shotgun sequence.
ACCESSION   DASUEV010000346 DASUEV010000000
VERSION     DASUEV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250248"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFV55_10910"
     CDS             <1..342
                     /locus_tag="VFV55_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX4944855.1"
                     /translation="GEKTLFWLLVVGLCSVISVTGLILNFPNWNQGREAMQLANLIHG
                     GAAILAMAMATFHIYLGTVGMQGAFNAMKTGYVDETWAKEHHQYWYDEVKAGKRPEKV
                     VAGSAQPATGD"
     gene            345..716
                     /locus_tag="VFV55_10915"
     CDS             345..716
                     /locus_tag="VFV55_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944856.1"
                     /translation="MKTRITALAIALGLGIALTTQAKIPPPPPLDEKGKAALEEKKAK
                     DAEAAAKAKAEQAAAEDNAVKNFQGNMKKAGKPIPKPTPIAVAAPAAPAAPAAAAPGK
                     ATPAKAEAPATPAAKAPAKKP"
     gene            727..1251
                     /locus_tag="VFV55_10920"
     CDS             727..1251
                     /locus_tag="VFV55_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX4944857.1"
                     /translation="MKALVAFALTAIAAAGCGSLGGGPGGPAAVANLAATKGNSASGT
                     VTFEKRGDVVRVSGTISGLKPDAEHGFHVHEKGDCSSGDGMGAGGHFNPLARNHGHHG
                     GSERHAGDMPNLRADAYGTATFSFDTAGITIGSGATDIVGRGLIVHRDPDDYKSQPAG
                     NAGPRIACAVIAKR"
     gene            complement(1310..2809)
                     /locus_tag="VFV55_10925"
     CDS             complement(1310..2809)
                     /locus_tag="VFV55_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944858.1"
                     /translation="MSRTPRPYLAATLAALALPAAAETAVTIYSSAQPGTLSPQTFRS
                     GGEGMTVPGYALVREDRRFDLAAGRNILRVPDVPALIDPTTVSFASLTDPKATRVVEQ
                     SFEFDLTSTQKLLSRYLDREITVEQERGQGVGTFSGTLVGTQGGLTLKAADGSVRVVN
                     GYSGIMLPGLPGGLISKPTLVWSIDAGKSGRHDARIAYQTGGMTWWADYNLAYAEPGP
                     GQCRLDVAAWVTIVNQSGAAYEDAKLKLIAGDVQRAQPRAYPAAAPAALGRVLEAKAQ
                     GFEEKAFFEYHLYTLGRPASLAQNSTKQIELFPTAAGVGCEKALVYHGQGTSYPAYGS
                     PMTDRNFGIQSNRKVDVYLRMKNSQANGLGVPLPAGKLRVSKRDDADASLEFIGEDLI
                     DHTARDETVQVKLGSAFDVVGERKQLDYRIDTSARWIEEEIEVRVRNQKPDEAVTVVV
                     KENLYRWSTWSVTRKSQEFEKQDSRTIHFPVRIAPRGEAVVRYTVRYTW"
     gene            2969..3811
                     /locus_tag="VFV55_10930"
     CDS             2969..3811
                     /locus_tag="VFV55_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX4944859.1"
                     /translation="MRFEGTDTYIADDDLKIAVNAAVTLQRPLLVKGEPGTGKTMLAE
                     EVARALDRPLYQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDEKVKDIANYIVKGVLWE
                     AFESEEKAVVLVDEIDKADIEFPNDLLRELDRMEFYVYETRQTVKARHRPVILITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMQAIVDVHFPGIRKQLVQAALESFFGIREMPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSDGHQQAIPPLYGALLKNEQDVHLFEQLLFLQ
                     RRGR"
     gene            3838..4548
                     /locus_tag="VFV55_10935"
     CDS             3838..4548
                     /locus_tag="VFV55_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944860.1"
                     /translation="MATKRKPAVKEAARRPAPKRPAQRKPSSKTPAKGKLSVRKRASR
                     GAIETLSGKALRLAGVGTDAVARATGKAWDQWLALLDKAGAVAMPHKAIASMLANKFG
                     VPSWWSQMVAVGYEQARGLRKVHEHSDGYAANSSRTIRAPLERLYGAWADPKLRALWL
                     GPAPITVKRSTDGKSMRITWTAGNSSVDVNFSAAGDGRSRVHVEHGMLADEKARDEQK
                     SFWGDALDRLKAMLEKAA"
     gene            4554..5729
                     /locus_tag="VFV55_10940"
     CDS             4554..5729
                     /locus_tag="VFV55_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX4944861.1"
                     /translation="MLIDFFFTVRKAGVPASLKEFLTLIEAMREGVVWGSVDDFYVLS
                     RATLVKDEAYFDRFDLAFSAWFKGVGTIPDALVKDIPADWLRKLAERTLSEEEKRLVE
                     ALGGWEKLMETLRQRLEEQKGRHQGGSKWIGTAGTSPFGAYGYNPEGIRIGQDRSRNR
                     RAVKVWDQREFRDYDTDLELGVRNIKLALRRLRRFARTGAPEVLDLPDTIRSTARNAG
                     WLDLKIVPELHNAVKVLLFLDVGGTMDDHIRIVEELFSAARTEFKNLEHFYFHNCVYE
                     SVWKDNLRRSRERIPTVDVMRKYGHDYKAIFVGDAAMSPYELEQPGGSVEHWNDEAGR
                     VWLERMLNAWPRAAWLNPVPEEHWNWTVSTENIRRILDGRMYPMTLAGLEGAMKSLS"
     gene            complement(5726..6793)
                     /locus_tag="VFV55_10945"
     CDS             complement(5726..6793)
                     /locus_tag="VFV55_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX4944862.1"
                     /translation="MTDETPVRYPFAETIPQPGEAIAVAPGVWWIRMPLPFALDHINL
                     WLLEDGDGWTIVDSGYGTAATWDLWERLLAGAMKDRPVKRIVVTHYHPDHVGSAAWLE
                     RRTGAPVWMTTSEFLSAHAARDDTAGFDRATGIDFFGRNGLNISSIPEKMRTGNFYKR
                     GVPEVPRHYRRIMHGDTLAIGAHDWRVICVFGHAPEHAALHCASLGILISGDQILPRI
                     TTNVGVWGNQPEANPLRLFLDSFARFADIPGATRVLPSHDRVFEGLHERIAQLRAHHA
                     ERLDSLAAACATPVTAFEILPVLFRRKLDEHQMGFAMGEAIAHLHYLHAEGRVRRIEE
                     GGVRRYVVSGTVSDTVSDTER"
     gene            complement(6825..9068)
                     /locus_tag="VFV55_10950"
     CDS             complement(6825..9068)
                     /locus_tag="VFV55_10950"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX4944863.1"
                     /translation="MALNARWFPKPSLNDVIRAAIGIAVVFFFLVHEAEWQEFRFLQQ
                     LELWAYDARIRLFLPKTRDTRVVILDIDEKSLNAEGRFPWPRNKLALMLKQLFEHYQV
                     RVVGFDIALPEPDTSSGLPIFEALAKGELKDNAEYQAFLRGARASLDYDKVLADEIAK
                     WPVVLGVAMGGKEDVAGVLPAPVFEARALGEAEYPHYTSTGYSGNIAILQKASTATGH
                     FYPALDIDGVTRRVPMFMLYKDGYYEALSLAVARTYLGNAAVKIVLDEPRRYGPRLEG
                     WMRSIRIGEVEVPLDRAMTATVPYRAAGGFRYVSATDVLRGTLPAGELKDKIIIVGTS
                     AQGLVDLRATPVQEDLPGVEVHATLVSGILDGTIKNRPPEVLGITAVLIALIGIPLAI
                     VLPRLSALWSTAAVAALFALVVATNLYLWNARNWVVPIASGLLMLVLLYFLNMVYGFF
                     AEARSRRLITGLFGTYVPKELVDEMAKNPGHYSMKGESREMTVLFSDIRDFTSISEGL
                     TPENLKELMNTYLTAMTEEVQEKRGTIDKYIGDAIMAFWGAPLADPDHATHALETGIA
                     MQKGLRGLDAPFAKRGWPPLHIGVGINCGMMNVGDMGSKFRRSYTVIGDAVNLASRLE
                     SLTKEYGVGILVSENIVRKATAFVYRDIDKVRVKGKHEGVGIFEPAGRQGEVGDETLR
                     EIERFHKALEAYRAQRWDDAEKMLKNLAYAAPEMKLYKLYLERVAHFRASPPPANWDG
                     VFVFTTK"
     gene            complement(9059..9943)
                     /locus_tag="VFV55_10955"
     CDS             complement(9059..9943)
                     /locus_tag="VFV55_10955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX4944864.1"
                     /translation="MIDVIIPAYRGLAQTRRCLESVLGAPVRSAHEVVVIDDASPEPE
                     LSAWLRSLAGGGRFSLLAHARNEGFVASVNEGMRLHADRDVVLLNSDTEVAGDWLDRI
                     AACAAREPRAGTVTPFSNNATICSYPLFAQANPMPANITTTRLDAAFARANAGKSVDI
                     PTAVGFCMFIARRCLSEVGPFDEAAFGRGYGEEVDFCMRASRAGWRHLLAADTFVYHE
                     GEVSFGGGAQDIRNRAQAIVDARYPEFQPRVREFVMEDPPRKLRERATAELASLPVYD
                     SRTGAATPTDKKSERSWP"
BASE COUNT         1709 a         3320 c         3468 g         1565 t
ORIGIN      
        1 ggcgagaaga cgctcttctg gctgctcgtg gtggggctct gctccgtgat cagcgtcacg
       61 ggcctgatcc tcaacttccc gaactggaac cagggccgcg aggcgatgca actcgcgaac
      121 ctcatccacg gcggcgccgc catcctcgcg atggcgatgg ccaccttcca catctacctg
      181 ggcaccgtcg gcatgcaggg cgcgttcaac gcgatgaaga ccggctacgt cgacgagacc
      241 tgggccaagg aacaccacca gtactggtac gacgaggtga aggccggcaa gcgtccggag
      301 aaggtcgtcg cgggctccgc acaaccggcc acgggagact gaacatgaag acgagaatca
      361 ccgcgctggc cattgcgctc ggcctcggca tcgcactcac cacccaggcc aagattccgc
      421 caccgccgcc cctggacgag aagggcaagg ccgccctcga ggagaagaag gcgaaggacg
      481 cggaggcggc cgcgaaggcc aaggccgagc aagccgcggc cgaggacaat gccgtcaaga
      541 acttccaggg caacatgaag aaggccggca agccgattcc gaagccgacc cccattgcgg
      601 tcgccgcccc ggcggcccca gcggccccgg cggccgctgc ccccgggaag gcgactccgg
      661 ccaaggcgga agcgccggcc acgcccgcag ccaaggcgcc ggcgaagaag ccctgaggag
      721 cgccgcatga aagcccttgt cgcgttcgcg ctgaccgcca tcgcggcggc gggctgcggg
      781 tcgctgggcg gggggccagg cggcccggcc gctgtcgcga atctcgccgc gaccaagggc
      841 aattccgcca gcggcacggt cacgttcgag aagcgcggcg acgtggtgcg ggtgtcgggc
      901 accatctcgg gcctcaagcc cgatgcggag cacggcttcc acgtccacga gaaaggcgat
      961 tgcagctccg gcgatggcat gggcgcgggc gggcacttca acccgctcgc caggaaccac
     1021 ggccaccacg gcggaagcga gcggcacgcg ggcgacatgc ccaacctgcg cgccgatgcc
     1081 tacgggaccg ccacgttctc gttcgacacg gcgggaatca ccatcggctc cggtgccacc
     1141 gacatcgtcg gcagggggct catcgtccat cgcgacccgg acgactacaa gagccagccg
     1201 gccggcaatg ccggtccacg gattgcctgc gcggtgatcg cgaaacgctg accggtcgat
     1261 acgccaatcg aaagggcggc ttcggccgcc ctttctattc gcagcgtcgc taccacgtgt
     1321 accggacggt atagcgcacc acggcctcgc ccctgggcgc aatgcgcacc gggaagtgga
     1381 tcgttcgcga atcctgcttc tcgaattcct gcgacttgcg cgtgaccgac caggtcgacc
     1441 accggtagag gttttccttc accacgaccg tcacggcctc gtcgggcttc tggttgcgca
     1501 cgcgaacctc gatctcctcc tcgatccacc tcgccgaggt gtcgatgcga tagtcgagct
     1561 gcttcctttc gcccacgacg tcgaaagccg accccagctt cacctgcacc gtctcgtcgc
     1621 gcgcggtgtg gtcgatgaga tcctcgccga tgaactcgag gctcgcgtcg gcatcgtcgc
     1681 gcttggagac gcgcagcttc ccggccggca gcggcacgcc cagcccgttg gcctgcgagt
     1741 tcttcatgcg caggtacacg tcgaccttcc tgttggactg gatgccgaaa ttgcgatcgg
     1801 tcatcggcga gccgtatgcc ggatagctgg tgccctgacc gtggtagacg agcgccttct
     1861 cgcagccgac gccggcggcc gtgggaaaga gctcgatctg cttcgtggag ttctgcgcga
     1921 gcgatgcggg gcgtcccagc gtgtagaggt ggtactcgaa gaacgccttc tcctcgaaac
     1981 cctgcgcctt cgcctcgagg acgcggccca acgccgcggg cgccgctgcc gggtaggcgc
     2041 gcggctgtgc acgctgcacg tcgcccgcga tgagcttcag cttcgcgtcc tcataggcag
     2101 cgcccgactg gttgacgatg gtcacccagg cggccacgtc gagccggcac tggccgggcc
     2161 ccggctcggc ataggcgagg ttgtagtccg cccaccacgt catgcccccc gtctggtagg
     2221 cgatccgggc atcgtgcctg ccggacttgc cggcatcgat gctccacacc agcgtgggct
     2281 tgctgatgag cccgcctggc aggcccggca gcatgatccc cgaatagcca ttcaccacgc
     2341 gcacgctccc gtcggctgcc ttcagcgtga ggccgccctg ggtgccgacc agcgtgccgc
     2401 tgaaggtgcc cacgccctgg ccgcgctcct gctcgacggt gatctcgcgg tcgaggtagc
     2461 gcgacagcag cttctgggtg ctggtgaggt cgaactcgaa gctttgctcg acgacccgcg
     2521 tggctttcgg atcggtgagg gaagcgaagg agacggtggt gggatcgatg agcgcgggca
     2581 cgtccggaac gcgaaggatg ttgcgaccgg cagcgagatc gaaacgtcgg tcctcgcgca
     2641 ccagggcata gccgggaacg gtcatgccct cgcccccgga acggaaggtc tgcggggaga
     2701 gcgtgcccgg ctgggcgctg gaatagatgg tcacggccgt ctctgcggcg gcgggaaggg
     2761 caagggcggc gagcgtggcg gcgaggtacg ggcgaggtgt gcgggacatg gcggtttcct
     2821 gtggtaggga agggacgcta cccttcgcga agcaaagaga agtgtcccca ttcgagttcg
     2881 aagcaaaggg tagcgtcccc tttcgagttc ctttcgagtt taacgggcgc cacgggcaaa
     2941 ggggttttcc cccgttatac ttcccggcat gagattcgag ggaaccgaca cctacatcgc
     3001 cgacgacgac ctgaagatcg ccgtcaacgc cgccgtcacg ctgcagcgcc cgctcctcgt
     3061 caagggcgag cccggcaccg gcaagaccat gctcgccgag gaagtggccc gcgcgctgga
     3121 ccggccgctc taccagtggc acatcaagtc gaccacgaag gcgcagcagg gcctctacga
     3181 gtacgacgcc gtgtcgcggc tgcgcgactc gcagctgggc gacgagaagg tgaaggacat
     3241 cgccaactac atcgtgaagg gcgtgctctg ggaggcgttc gagagcgagg aaaaggcggt
     3301 ggtgctcgtc gacgagatcg acaaggccga catcgaattc cccaacgacc tgctgcgcga
     3361 gctcgaccgc atggagttct acgtctacga gacgcgccag acggtgaagg cgcgccaccg
     3421 gccggtcatc ctcatcacgt ccaacaacga gaaggagctg cccgacgcgt tcctgcgccg
     3481 ctgcttcttc cactacatcc gcttccccga caaggagacg atgcaggcca tcgtcgacgt
     3541 gcatttcccg ggcatccgca agcagctcgt gcaggcggcc ctggagagct tcttcggcat
     3601 ccgcgagatg ccgggcctga agaagaagcc ctcgacctcc gagctcctcg actggctgaa
     3661 gctgctgctg gccgaggaca tcccgcccga ggcgctgcgg agcgacggcc accagcaggc
     3721 cattccgccg ctctacggcg cgctcctcaa gaacgagcag gacgtccacc tgttcgagca
     3781 gctgcttttc ctgcaacgcc gcggccgcta ggcccggccc cgcgaaatgg aggcgccatg
     3841 gccacgaagc gcaagccggc cgtgaaggag gccgcccgca ggcctgcacc caagagaccg
     3901 gcgcaacgca agccctcctc gaagacgcca gcgaagggga aactgtccgt gcgcaagcgt
     3961 gcatcccgcg gcgccatcga gacgctttcc ggcaaggctc tgcgcctggc cggcgtgggc
     4021 actgacgcgg tggcaagggc caccgggaag gcctgggacc agtggctcgc cctcctcgac
     4081 aaggccgggg cggtcgcgat gccgcacaag gccatcgcct cgatgctcgc gaacaagttc
     4141 ggcgtgcctt cctggtggag ccagatggtc gcggtgggct acgagcaggc gcgcggcttg
     4201 cgcaaggtcc acgagcacag cgacggatac gcggcgaatt cctcgcggac gatccgggcg
     4261 ccgctcgagc gcctgtacgg cgcctgggcg gatccgaagc tgcgcgcgct ctggctcggg
     4321 ccggcgccga tcaccgtgaa gcgctcgacc gacgggaaat cgatgcgcat cacctggacc
     4381 gcgggcaact cgagcgtgga cgtgaacttc tccgcagccg gcgacggccg gagccgcgtg
     4441 cacgtcgaac acggcatgct cgccgacgag aaggcccggg acgagcagaa gtccttctgg
     4501 ggcgatgcgc tggaccgcct caaggccatg ctggaaaagg cggcgtagcg cccgtgctca
     4561 tcgacttctt cttcaccgtt cgcaaggccg gggtgccggc gagcctgaag gagttcctca
     4621 cgctcatcga ggcgatgcgc gaaggcgtgg tgtgggggag cgtcgacgat ttctacgtgc
     4681 tgtcgcgcgc cacgctcgtg aaggacgagg cctatttcga ccgtttcgac ctggcgtttt
     4741 ccgcctggtt caagggcgtg ggcacgatcc cggatgcgct ggtcaaggac attcccgcgg
     4801 actggctgcg caagctcgcc gaacgaaccc tttccgagga ggaaaagcgc ctcgtcgagg
     4861 cgctgggcgg ctgggagaaa ctcatggaga cgttgcgcca gcggctcgag gagcagaaag
     4921 gccgccacca gggcggctcc aagtggatcg gcaccgccgg cacctcgccc ttcggcgcgt
     4981 acggctacaa ccccgagggc atccgcatcg ggcaggaccg ctcccgcaac cgccgcgcgg
     5041 tgaaggtctg ggaccagcgc gagttccgcg attacgacac ggacctcgaa ctgggcgtga
     5101 ggaacatcaa gctggcgctc cggcggctgc ggcgcttcgc acggaccggg gctcccgagg
     5161 tgctggacct tcccgacacc atccgctcga cggcgaggaa cgccggctgg ctcgacctca
     5221 agatcgtccc ggaactgcac aacgcggtga aggtgctgct attcctcgac gtgggcggca
     5281 cgatggacga ccacatcagg atcgtcgagg agctgttcag cgccgcgcgc accgagttca
     5341 agaacctcga gcacttctac ttccacaact gcgtctacga gtcggtgtgg aaggacaacc
     5401 tgcgccgcag ccgcgagcgc atcccgacgg tcgacgtgat gcgcaagtac ggccacgact
     5461 acaaggcgat cttcgtgggg gatgccgcga tgagccccta cgagcttgag cagcccggcg
     5521 gcagcgtcga gcactggaac gacgaggcgg gccgcgtgtg gctggagcgg atgctgaacg
     5581 cctggccgcg cgccgcgtgg ctcaacccgg tgccggagga gcactggaac tggaccgtct
     5641 ccacggagaa catccggcgc atcctcgacg gccggatgta cccgatgact ctcgccgggc
     5701 tcgagggcgc gatgaagtcg ctcagctagc gttcggtgtc agacacggtg tctgataccg
     5761 tgcctgacac cacgtagcgc cgcacgccgc cttcctcgat gcggcgcacg cgcccttcgg
     5821 catgcagata gtgcaggtgc gcgatcgcct cgcccatcgc gaagcccatc tggtgctcgt
     5881 cgagcttgcg cctgaagagg accggcagga tctcgaaagc ggtgaccgga gttgcgcagg
     5941 cggccgcgag gctgtcgaga cgctccgcgt ggtgcgcgcg tagctgggcg atgcgttcat
     6001 gaagaccctc gaagacgcgg tcgtgcgagg gaagcacgcg ggtcgcgccc gggatgtccg
     6061 cgaagcgcgc gaacgaatcg aggaagagcc gcaacgggtt ggcctccggc tggttgcccc
     6121 agacacccac gttggtcgtg atcctgggca ggatctggtc gccggaaatc aggatgccga
     6181 gcgaggcgca gtgcagggcc gcgtgctccg gggcgtgccc gaaaacgcag atcacccgcc
     6241 agtcgtgcgc gccgatcgcg agtgtgtcgc cgtgcatgat gcgccggtag tgccgcggga
     6301 cttccggcac gccgcgcttg tagaaattgc cggtgcgcat tttttcgggg atggacgaga
     6361 tgttcaggcc gttgcgcccg aagaaatcga tgcccgtggc gcggtcgaag ccggccgtgt
     6421 cgtcgcgcgc ggcgtgcgcg gagaggaatt ccgaggtcgt catccatacc ggcgccccgg
     6481 tccgccgctc gagccacgcc gcgcttccca cgtggtcggg gtggtaatgg gtcaccacga
     6541 tgcgcttcac cgggcgatcc ttcatcgcgc cggcgaggag ccgctcccac aggtcccacg
     6601 tcgccgcggt gccgtagccg ctgtccacga tcgtccagcc gtcgccgtcc tcgaggagcc
     6661 acaggttgat gtggtccagc gcgaaaggca gcggcatgcg gatccaccac acgccggggg
     6721 cgacggcgat cgcttcgccg ggttgcggga tcgtttcggc gaaagggtag cggaccgggg
     6781 tctcgtcggt catggcatgg gcgccgggga gcggcgcggg caggttactt ggtggtgaac
     6841 acgaacacgc cgtcccagtt cgcgggcggc gggcttgcgc ggaagtgtgc cacgcgctcg
     6901 agatagagct tgtagagctt catctcgggg gcggcgtagg caaggttctt cagcatcttc
     6961 tcggcgtcgt cccagcgctg cgcgcggtag gcttcgagcg ccttgtggaa gcgctcgatt
     7021 tcccggagcg tctcgtcgcc cacctcgccc tgccggccgg cgggctcgaa gattcccacg
     7081 ccctcgtgct ttcctttcac gcgcaccttg tcgatgtcgc gatacacgaa cgccgtcgcc
     7141 ttgcgcacga tgttctccga gaccaggatg ccgacgccgt actccttggt aaggctctcc
     7201 aggcgcgagg cgaggttcac ggcgtcgccg atcacggtgt aggagcgccg gaacttcgat
     7261 cccatgtcgc ccacgttcat catgccgcag ttgattccga cgccgatgtg caggggcggc
     7321 cagccgcgct tggcgaacgg ggcgtccagg ccgcgcaggc ccttctgcat ggcgatgccg
     7381 gtctcgaggg cgtgcgtggc gtggtcgggg tcggcgagag gcgcccccca gaaggccatg
     7441 atcgcgtcgc cgatgtactt gtcgatcgtg ccgcgcttct cctgcacctc ctcggtcatc
     7501 gccgtgaggt aggtgttcat cagttccttg agattctccg gcgtgaggcc ctcggagatg
     7561 gaggtgaagt cgcggatgtc ggagaagagc acggtcatct cgcggctctc gcccttcatc
     7621 gagtaatggc cggggttctt tgccatctcg tcgaccagct ccttcggcac gtaggtgccg
     7681 aagagaccgg tgatgagccg gcgcgagcgc gcttccgcga agaagccgta gaccatgttg
     7741 aggaagtaga ggagcaccag catcagcagc ccggaggcga tgggcaccac ccagttcctc
     7801 gcgttccaca gatagaggtt tgtcgcgacc accagggcga agagggcggc gaccgcggcc
     7861 gtggaccaca gggcggaaag gcgcggcagg acgatggcca gcgggatgcc gatcagggcg
     7921 atcagcacgg ccgtgatgcc gagcacttcg ggcgggcggt tcttgatcgt gccgtcgagg
     7981 atgccggata cgagcgtggc gtggacctcg acgcccggca ggtcctcctg caccggggtc
     8041 gcgcgcaggt ccacgaggcc ctgggccgaa gtgcccacga tgatgatctt gtccttcagc
     8101 tcgccggccg gcagcgtgcc gcgcaagacg tcggtggccg agacgtagcg gaagccgccg
     8161 gccgcgcggt aggggaccgt ggcggtcatc gcgcgatcga gggggacctc gacctcgccg
     8221 atcctgatgg agcgcatcca tccctccagc ctcgggccgt acctgcgcgg ctcgtcgagg
     8281 acgatcttca ccgcggcgtt gccgaggtag gtgcgcgcga cggcgagcga cagcgcctcg
     8341 tagtagccgt ccttgtagag catgaacatc ggcacgcgcc gcgtgacgcc gtcgatgtcg
     8401 agggcagggt agaaatgccc cgtggccgtc gacgccttct gcagaattgc gatgttgccg
     8461 ctgtagcccg tcgaggtgta gtgggggtac tcggcttcac ccagcgccct ggcctcgaat
     8521 accggcgccg gcagcacgcc cgcgacgtcc tccttgccgc ccatggccac gccgaggacg
     8581 accggccact tcgcgatctc gtcggccagg accttgtcgt agtcgagcga cgcacgcgca
     8641 ccgcgcagga acgcctggta ctccgcattg tccttgagct cgcccttcgc gagcgcctcg
     8701 aagatgggca ggcccgagct cgtgtcgggc tcgggcagcg cgatgtcgaa ccccacgacg
     8761 cgtacctggt agtgctcgaa gagctgcttc agcatcaacg ccagtttgtt gcgcggccac
     8821 gggaagcgac cctcggcatt gaggctcttc tcgtcgatgt cgaggatgac gacgcgcgtg
     8881 tcgcgggtct tgggcaggaa gaggcgaatc ctcgcgtcgt aggcccagag ctcgagctgc
     8941 tgcaggaagc ggaactcctg ccactccgcc tcgtggacga ggaagaagaa gacgaccgcg
     9001 atcccgatcg ctgcgcggat gacgtcgttc agggagggct tcgggaacca gcgggcattc
     9061 agggccacga tctctcgctt ttcttgtcgg tcggtgtggc tgccccagtc cgggaatcat
     9121 acaccggcag ggacgcgagt tccgcggtcg cgcgctcccg cagcttgcgc ggcggatcct
     9181 ccatcacgaa ttcgcgcacg cgcggctgga attccgggta gcgggcgtcg acgatggcct
     9241 gggccctgtt gcggatatcc tgcgccccgc cgccgaacga tacctcgccc tcgtggtaca
     9301 cgaaggtgtc ggcggccagg aggtggcgcc acccggcgcg cgacgcgcgc atgcagaaat
     9361 cgacctcctc gccgtatccg cggccgaagg cggcctcgtc gaagggaccc acctccgaga
     9421 ggcatcgccg cgcgatgaac atgcagaagc cgacggccgt cgggatatcg accgacttcc
     9481 ccgcgttggc ccgggcgaag gccgcatcca gccttgtcgt ggtgatgttc gccggcatgg
     9541 ggtttgcctg cgcgaaaagg ggatagctgc agatcgtcgc gttgttcgaa aatggggtca
     9601 ccgtgccggc gcggggctcg cgagcagcgc aggccgcgat ccggtccagc cagtccccgg
     9661 cgacctcggt gtcgctgttg aggagcacca cgtcgcgatc cgcgtgcagg cgcatgcctt
     9721 cgttcaccga ggccacgaaa ccctcgttcc gggcatgcgc aagaagcgag aaccgcccgc
     9781 cgccggcgag actgcgcaac caggcggaaa gctccggctc ggggctggcg tcatcgatga
     9841 ccacgacctc gtgcgccgaa cggaccgggg cgccgaggac gctttccagg cagcggcgcg
     9901 tctgggcgag tccgcggtag gcgggaatga tgacgtcgat caacgggagc ggcagggagg
     9961 gggttgatgg ctgattttat cgcgccgcct ggacttgcac gccacggatg gcgtccagga
    10021 taccccccca ggcatatcgg gaattcggtg aagacgcgtt ct
//
LOCUS       DASUEV010000347         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_663882, whole genome shotgun sequence.
ACCESSION   DASUEV010000347 DASUEV010000000
VERSION     DASUEV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663882"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VFV55_10960"
     CDS             complement(<1..800)
                     /locus_tag="VFV55_10960"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX4944865.1"
                     /translation="MLDQIRTFAKYRIVKWFFAIFLIIPFGLFGVDYYFRTPMGGDSV
                     ATVGRLRVSQGDFDQALRQQQETLQAQFGRNFDASLMENPELRRSVLDRVINERLVSV
                     GAERAGVRIDDKQLAARIAAEPGFQEDGKFSRQRYEAIARSQGYSVVGLDERLREDMR
                     LARYRDAIAQTAIVPRSTLDGFIRLSEQSREVSLVNVSPDEFISTVKPAEAQLKAYYD
                     AHAKEFAVPEQVRVEYVELSMDALAAQAPVDPEDVKKFYESNKSRFVQR"
     gene            complement(878..954)
                     /locus_tag="VFV55_10965"
     tRNA            complement(878..954)
                     /locus_tag="VFV55_10965"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(918..920),aa:Asp,seq:gtc)
     gene            complement(1014..1089)
                     /locus_tag="VFV55_10970"
     tRNA            complement(1014..1089)
                     /locus_tag="VFV55_10970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1054..1056),aa:Val,seq:tac)
     gene            complement(1100..1372)
                     /locus_tag="VFV55_10975"
     CDS             complement(1100..1372)
                     /locus_tag="VFV55_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX4944866.1"
                     /translation="MNKSDLIEAIAKSADISKAAAGRALEGALAAIRTSLRKGSPVSL
                     VGFGTFHVGKRASRSGRNPRTGAPIKIKAAKVPKFRAGKALRDALN"
     gene            complement(1407..>2549)
                     /locus_tag="VFV55_10980"
     CDS             complement(1407..>2549)
                     /locus_tag="VFV55_10980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX4944867.1"
                     /translation="DEVDKMGMDFRGDPSSALLEVLDPEQNHTFVDHYVEVEYDLSDV
                     MFVATANTLNIPAALLDRMEVIRLSGYTESEKVHIAREYLLGKQLKANGLKAGELTVT
                     DSALRDIARYYTREAGVRGFDRELAKISRKVVKSILIKKKDGATVTVNAKNLDKYLGV
                     RKFTYGIAEKENQVGQVTGLAWTEVGGELLTVEAAKVPGKGNTVTTGKLGEVMQESIK
                     AAISVVRARAKRLGIPEDFYQNLDLHIHLPEGATPKDGPSAGIAITTALVSVLTGIPV
                     RCDVAMTGEITLRGEVLPIGGLKEKLLAAHRGGVKTVLIPFENVKDLAEVLPEEVKGQ
                     IEILPVRWIDEVLERALETKPVPLAESAPANPPPAAAPVPGVVVKH"
BASE COUNT          376 a          849 c          856 g          468 t
ORIGIN      
        1 cgctgcacga agcgcgactt gttcgattcg tagaacttct tcacgtcctc cggatcgacc
       61 ggcgcctgcg cggccagggc gtccatcgac agctcgacgt attccacgcg cacctgctcg
      121 ggcaccgcga actccttcgc atgcgcgtcg taataggcct tgagctgggc ctcggccggc
      181 tttacggtcg agatgaattc atcggggctc acgttgacga ggctcacctc gcggctctgc
      241 tcggacaggc ggatgaaccc gtcgagggtc gagcgcggga cgatcgccgt ctgggcgatg
      301 gcatcgcggt agcgggcaag gcgcatgtcc tctcgcaggc gctcgtcgag gcccaccacg
      361 gaatagccct gggagcgcgc gatggcttcg tagcgctgcc gcgagaactt gccgtcttcc
      421 tggaagccgg gctcggcggc gatgcgcgcg gccagctgct tgtcgtcaat gcgcacgccc
      481 gcgcgttcgg cgccgacgga gaccaggcgc tcgttgatca cgcggtccag aacggaccgg
      541 cgcagctccg ggttctccat gagggaggcg tcgaaattgc gcccgaactg ggcctgcagc
      601 gtctcctgct gctggcgcag cgcctggtcg aagtcgccct ggctcacgcg cagccttccc
      661 acggtggcga ccgaatcgcc gcccatgggg gtgcggaagt agtagtcgac gccgaagagg
      721 ccgaagggaa tgatcaggaa gatcgcgaag aaccacttga cgatcctgta cttggcgaac
      781 gttcgaatct ggtcgagcat ggcgatcggt gcctctggcc cgcaaccggg gcgttcatgc
      841 aaaaagggcg aacccgcacg ggttcgccct ggtggactgg cggagtggac gggactcgaa
      901 cccgcgaccc ccggcgtgac aggccggtat tctaaccaac tgaactacca ctccatgcaa
      961 ctctggcccc gctgcccccg gcctgcaggc cccgaggccg cgccggcgac tgctggtggg
     1021 tgctgagggt ctcgaacccc cgactttcgc cttgtaaggg cgacgctcta ccaactgagc
     1081 taagcacccg ggaagcccgt tagtttagcg catcgcggag tgcttttcca gcgcggaact
     1141 tgggtacttt cgcggccttg atcttgatcg gcgccccggt gcgcgggttg cgccccgaac
     1201 gggaggcgcg ctttcccacg tggaaggtgc cgaagcccac caggctcacg gggctcccct
     1261 tgcgaagcga ggtcctgatc gcggccagcg cgccctcgag ggcgcggccg gcggccgcct
     1321 tggagatgtc ggcgctcttg gcgatcgcct cgatgagatc cgatttgttc acttgttgtt
     1381 ctcccttgcg gtggttcgaa tgcgggtcag tgcttgacga cgacgcccgg gacgggcgcg
     1441 gcggcaggcg gcgggttggc cggggccgat tcggcgagcg gcacgggctt ggtctcgagc
     1501 gcgcgctcga ggacctcgtc gatccagcgc accggcagga tctcgatctg gcccttcacc
     1561 tcctcgggca gcacctcggc caggtccttc acgttctcga aggggatgag caccgtcttc
     1621 accccgccac ggtgagcggc gagcagcttt tccttgagcc cgccgatcgg caggacctcg
     1681 ccgcgcagcg tgatctcgcc cgtcatcgcg acgtcgcagc gcaccgggat gccggtgagc
     1741 accgacacca gcgccgtggt gatcgcgatg ccggcactcg ggccgtcctt gggcgtcgcg
     1801 ccctcgggca ggtggatgtg caggtcgagg ttctggtaga agtcctccgg aatgcccagg
     1861 cgcttggcgc gcgcgcgcac caccgagatc gcggccttga tggattcctg catcacctcg
     1921 ccgagcttgc cggtggtgac ggtgttgccc ttgccgggca ccttggccgc ttccaccgtg
     1981 aggagctcgc cgccgacttc ggtccacgcg aggccggtga cctggcccac ctggttttcc
     2041 ttctcggcga tgccgtaggt gaacttgcgc acgcccaggt acttgtcgag gttcttcgcg
     2101 ttcaccgtga cggtcgcgcc atccttcttc ttgatgagga tggacttcac caccttgcgg
     2161 ctgatcttgg ccagctcgcg gtcgaagccg cgcacgcccg cctcccgcgt gtagtagcgc
     2221 gcgatgtcgc gcagcgccga atcggtcacg gtgagctcgc ccgccttcag gccgttggcc
     2281 ttgagctgct tgcccagcag gtactcgcgg gcgatgtgca ccttctcgct ctccgtatag
     2341 cccgagaggc ggatcacttc catccggtcc agcagcgccg ccgggatgtt gagcgtgttg
     2401 gcggtggcga cgaacatcac gtccgagagg tcgtactcca cctcgacgta gtggtcgacg
     2461 aaggtgtggt tctgctccgg gtcgagcact tcgagcagcg ccgaggacgg gtcgccgcgg
     2521 aagtccatgc ccatcttgtc cacctcgtc
//
LOCUS       DASUEV010000348         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_664559, whole genome shotgun sequence.
ACCESSION   DASUEV010000348 DASUEV010000000
VERSION     DASUEV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664559"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3094)
                     /locus_tag="VFV55_10985"
     CDS             complement(<1..>3094)
                     /locus_tag="VFV55_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEX4944868.1"
                     /translation="NGRYHVMVTNAGGGSSRWKDLAVTRWREDSTCDNWGTFCYIRDV
                     ASGDFWSTAHQPTLRRPAHYEAIFTEARAEFRRRDNDFDSHTEIVVSPEDDIELRRLR
                     ITNRSRGRRTIDVTSYAEVALAPPAADALHPAFSKLFVQAEVIAQRQAILCTRRPRSR
                     DESSPWMFHLMAVHGPASLDVSFETDRMAFIGRGRTAAAPRAMTQAGRLSGSQGSVLD
                     PIVAIRHRMTLEPQQTATIDIASGMAETRDAALGLVGKYQDRRLADRVFDLAWTHSWV
                     TLRQINATESDAQLYARLASSVIYANASLRAGAGVLLRNRRGQSGLWSYAISGDLPIV
                     LLQIGDSANIDLVRQLVQAHAYWRLKGLAVDLVIWNEDRGGYRQLLQDQIMGLIAAGV
                     EAHVMDRPGGIFVRRGEQIADEDRILLQSVARAIITDRRGSLVEQVSRRGPPEVRVPR
                     LVPTRNHRDEALPAEAPARELILSNGLGGFTPDGAEYVITTNAGQATPAPWVNVLANP
                     EFGTVISESGLAYTWSENAHEFRLTPWHNDPVSDASGEAYYLRDEESGHFWSPAPLPS
                     RGAGAYVTRHGFGYSVFEHVEDEIHSELWVYVAIDASVKLAVLKVRNASGRPRRLSAT
                     GYVEWVLGDLRPKTTMHVITESDSKSGALLARNAYNTEFPDRVAFFDVDAPIRSVSGD
                     RTEFLGRNGTLRNPAAMARSRLSGKLGAALDPCAAIQVPMELVEGEEREIVFRLGVGR
                     NAADALGLVQRFRGPTAARGALEAVRAHWKRMLGAVQVQTPDEALNVLANGWLLYQTV
                     ACRLWARSGYYQSGGAFGFRDQLQDVMALVHAEPGLMREHLLRCAGRQFVEGDVQHWW
                     HPPSGRGVRTRCSDDYLWLPLATARYVNATADGGVLDEAVPFLEGRPVNEGDDSYYDM
                     PRRSAESASVYQHCVRAIEHGLHYGAHGLPLMGSGDWNDGMNLVGIKGTGESVWLGFF
                     LCSVLKQFAGVARLKGDAAFAAKCDLEEAELRRNLEKHGWDGQWYRRAYFDDGSPLGS
                     S"
BASE COUNT          459 a         1077 c         1073 g          485 t
ORIGIN      
        1 cgacgatccg agcggcgagc cgtcatcgaa gtacgcgcgc cggtaccatt ggccgtccca
       61 cccgtgcttc tcgaggttgc ggcgcagttc ggcctcctcc aggtcgcact tcgcggcgaa
      121 tgccgcgtcg cctttcaggc gcgcgacgcc ggcgaactgc ttcagcacgg agcagaggaa
      181 gaagcccagc cagacgctct cgccggtgcc cttgatcccg accaggttca tgccgtcgtt
      241 ccagtcgccg gagcccatga gcggcagccc gtgcgccccg tagtgcaggc catgctcgat
      301 ggcgcgcacg cagtgctggt acacgctggc ggattccgcg gaccggcggg gcatgtcgta
      361 gtacgagtcg tccccctcgt tcaccgggcg cccttccagg aaggggaccg cctcatcgag
      421 aacgccaccg tccgcggtcg cattcacgta gcgtgccgtc gccaacggca gccagaggta
      481 gtcgtcggaa cagcgcgtgc gcacgccccg gcccgacggc ggatgccacc agtgctggac
      541 atctccttcc acgaactggc gccccgcgca gcgcaacagg tgctcgcgca tcagcccggg
      601 ctcggcgtgc acgagcgcca tcacgtcctg caactggtcg cggaagccga aggccccgcc
      661 ggactggtag tagccgctgc gcgcccacag gcggcacgcc accgtctggt agagcagcca
      721 gccgttggcc aggacgttga gcgcctcgtc cggcgtctgg acctgcacgg cgccgagcat
      781 gcgcttccag tgcgcgcgca ccgcttccag cgcgccgcgg gcggcggtcg gtccgcggaa
      841 gcgctgcacg aggccgagcg catccgcggc attccggccg acgccgaggc ggaagacgat
      901 ctcgcgttct tcgccctcga ccagttccat cggaacctgg atggcggcgc acgggtcgag
      961 tgccgcgccc agcttgccgg aaaggcgcga tcgcgccatc gcggccgggt tgcgcagggt
     1021 gccgttgcgc ccgaggaatt cggtgcggtc gccgctcacg ctgcggatcg gcgcatcgac
     1081 gtcgaagaag gcgacgcgat cggggaattc cgtgttgtac gcgttgcgcg ccaggagcgc
     1141 gccgctcttc gagtcgcttt cggtgatcac gtgcatcgtc gtcttcggcc gcaagtcccc
     1201 cagcacccat tccacgtagc ccgtggccga gaggcggcgt ggccggcccg aggcattgcg
     1261 gaccttgagc accgcgagct tcaccgaagc gtcgatggcc acgtataccc agagctcgga
     1321 atggatttcg tcctcgacgt gctcgaagac gctgtagccg aaaccgtggc gggtgacata
     1381 ggcgcctgca ccgcggctgg gcagcggtgc gggcgaccag aaatggccgg actcctcgtc
     1441 gcgcaggtag taggcctcgc cgctcgcatc gcttacgggg tcgttgtgcc agggcgtgag
     1501 gcggaactcg tgggcgttct cgctccaggt gtaggcgagc ccgctctccg agatcaccgt
     1561 gccgaactcc gggttcgcaa gcacgttcac ccagggtgcc ggggttgcct ggccggcgtt
     1621 cgtcgtgatt acgtattcgg cgccgtcggg cgtgaatccc ccgagcccgt tggacaggat
     1681 caattcccgt gcgggcgctt cggcgggcag ggcctcgtcg cgatggtttc gcgtcggcac
     1741 gaggcgcgga acccgcactt ccggcgggcc gcgccgggac acctgctcca ccaggctccc
     1801 gcggcggtcg gtgatgatgg cgcgcgccac cgactggagc aggatgcggt cctcgtcggc
     1861 gatctgttcc ccccgccgca cgaagatccc gccgggtcga tccatgacgt gcgcttccac
     1921 gccggcggcg atcaggccca tgatctggtc ctgcaggagc tggcgatacc cgccgcggtc
     1981 ctcgttccag atcacgaggt ccaccgccag gcccttcagc cgccagtacg cgtgcgcctg
     2041 gaccagctgg cggacgagat cgatgttcgc cgagtccccg atctgcagca ggacgatcgg
     2101 caggtcgccg gagatcgcgt agctccacaa tcccgactgg ccgcggcggt tcctgaggag
     2161 aacgcccgca cccgcccgca acgaggcgtt ggcgtagatg acggaactgg caaggcgcgc
     2221 gtagagctgc gcatcggatt ccgtggcatt gatctggcgc agcgtcaccc agctgtgcgt
     2281 ccacgccagg tcgaacacgc gatcggccag gcgccggtcc tggtacttgc cgaccaggcc
     2341 cagcgcggcg tcgcgcgttt cggccatgcc cgaggcgatg tcgatcgtcg ccgtctgctg
     2401 cggctcgagc gtcatgcggt ggcggatcgc gacgatcgga tcgaggaccg acccctggct
     2461 gccggaaagc cttccggcct gggtcatggc gcgcggagcg gcggccgtcc gtccgcggcc
     2521 gatgaatgcc atgcggtccg tctcgaagga cacgtccagc gacgccggcc cgtggacggc
     2581 catgaggtgg aacatccacg gcgaggattc gtcccgcgag cggggacgcc gcgtgcacag
     2641 gatggcctgc cgctgcgcga tgacctcggc ctgcacgaag agcttggaaa acgccgggtg
     2701 cagcgcatcc gccgcgggcg gcgccagggc gacctccgca tagctcgtga cgtcgatcgt
     2761 ccgccgcccc cgcgagcggt tggtgatgcg cagccggcgc agctcgatgt cgtcctccgg
     2821 cgaaacgacg atctcggtat gcgaatcgaa gtcgttgtcg cgccggcgga actcggcgcg
     2881 cgcctcggtg aatatggcct cgtagtgcgc cgggcgcctg agcgtcggct ggtgcgctgt
     2941 cgaccagaaa tcgccgctcg ccacgtcgcg gatgtagcag aacgtgcccc agttgtcgca
     3001 ggtgctgtcc tcgcgccagc gggtgacggc gaggtccttc caccgcgaac tgccgccgcc
     3061 cgcgttggtg accatgacgt gatacctgcc gttc
//
LOCUS       DASUEV010000349        11598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_325889, whole genome shotgun sequence.
ACCESSION   DASUEV010000349 DASUEV010000000
VERSION     DASUEV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325889"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFV55_10990"
     CDS             <1..702
                     /locus_tag="VFV55_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEX4944869.1"
                     /translation="TLADLLLSQGRHEEALAVLKDLSGRDARNLRLLRMRLDAETALR
                     RWDEVLATASALAKLGGLSAQEAAASRRAAHLGNLKRKATDAAALAAYWKQIPSELRV
                     DSAIAATAASYHLSLGGAAEAQGIVESALEAGWDARLVALYADCAGESALPLIERAEK
                     WLRANARDPALLLTLGKLCMHQGLWGKAQSYIEASLALEPTHEGHMTLAALMERLGKP
                     QEAVRHFRRSAELAG"
     gene            747..1325
                     /locus_tag="VFV55_10995"
     CDS             747..1325
                     /locus_tag="VFV55_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX4944870.1"
                     /translation="MTREYFVLQHHALAPGARVGTPLPPARLTPESPALEAMTDLQRI
                     EAATIAPHRSIVECNTVMIVHSIRLLFVEDADRSVVGVITATDLLGEKPVRFMRERGV
                     RHHEILVSDLMTPASALEALDLQSVTTAQVGHIVATLKSVGRQHTFVTDEGGKRICGL
                     FSATDIARRLGAEVPTHEVAASFAQIEAALSR"
     gene            1322..2974
                     /locus_tag="VFV55_11000"
     CDS             1322..2974
                     /locus_tag="VFV55_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4944871.1"
                     /translation="MTDGHAEAVRAFRWQVPARCNIADSACRRWAADRARFALYWEDE
                     SGATAAFTYWDLLQAANRLSNALVALGVARGDRVAILLPQRPETVVAYLACFQMGAIA
                     VPLSFLFGPDALEYRLADSAAKVAIADPETLPNLWAVRERLPELAHVIGVAGARESGV
                     HAWESLLERASRRFDCVETGPRDPAVIIYTSGTTGPPKGALMPQSTLIGNLPGFEYSH
                     DGFPQEGDLFWSPADWAWTGGLWDALMPTLHHGQAILGYRGRFDPEKAFHLLDKYAVR
                     NAFLFPTALKMMRKAVDHPRARFDLDLRSMMSGGEPVGPALFEWCRDELGVTVNEIFG
                     QTEMNYIVGNSHRVWPVKPGSMGRPYPGHRIAVIGDDGLEVPAGTVGDVAVHRVGTDG
                     TPDPVFFLGYWNNPEATAKKYTGDWCRTGDHASADADGYLWYQGRGDDMFKSAGYRIG
                     PAEIENCLVKHEAVANAAVVPSPDETRGSVVKAFVLLAPGRTPSPALEESLRAHVKAH
                     LAPWQQPREIEFLAELPMTTTGKVQRRVLREREALKKRGGTP"
     gene            3135..4826
                     /locus_tag="VFV55_11005"
     CDS             3135..4826
                     /locus_tag="VFV55_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX4944872.1"
                     /translation="MPLDQISSSAAITAADIAAARAESAQTGQPMIDVLERQSGLSGD
                     DFVAALGRALAFPAVRMDELREGDAAFDVVPFPEAVERGCVPIRRNGALALVHGDPFR
                     AGTRAWMEERVGVPFSWLLAHPSTIAAFFRRFEESARAMDDVVQYATDPEEGGAQRGA
                     TLSLQSIGEDESLAVRLVNSTLYDALKSGASDIHLETDPGGLSVKYRVDGVLSAVAKP
                     RGIDFAEQVISRVKVMAELDIAERRVPQDGRFRIVSQGRAVDLRVSVMPSIHGEDAVL
                     RILDKQSLADEIRGLRLDRLGFDPDIVNATLALASLPYGMLLVTGPTGSGKTTTLYAA
                     VTEINTGQDKIVTIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKILVGEI
                     RDPDTAQIAVQAALTGHLVLTTIHANSAFDVIGRVGNMGVDAFSFASATNGILAQRLV
                     RVTCEHCAEPQTHPEELLRASGIDPADAGRYLFRAGRGCGHCRGSGFRGRRAIAELLH
                     LDEEIRELIARRESIRAIRQAARANGTRMLRESALDLVLRGETTLQEINRVTAVA"
     gene            4807..5607
                     /locus_tag="VFV55_11010"
     CDS             4807..5607
                     /locus_tag="VFV55_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944873.1"
                     /translation="MLLPWRDELHIAIRPTELVRVRRTFGARGVRAEHATSPVEPAAD
                     NRAWRSAIDVLAQGVAQLAERPASVRIVLSNHFVRYAIAPWRDDMRSPAEREGFIRHC
                     FRENYGDAAEGWVIRENPEPYGRASLACAVDRELVDAVRDVFRGTRLSLAAIQPLFMA
                     AFNKYRKALGSTGCFLVYEKGRLCGATYADGEWRSALAVRVEEGCFNAATIERELLLQ
                     GVPEDFPVFLCQVDDASDPGTFARPVKRLDPPGLDEPDFTAALLGGGE"
     gene            5604..6125
                     /locus_tag="VFV55_11015"
     CDS             5604..6125
                     /locus_tag="VFV55_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX4944874.1"
                     /translation="MKRLRLDLAGPRGSSAGWILLGLALVALADLGASWRQLDEEIAA
                     AEQQAARPRARVGEHTARGARGGEGAAGDLRDADRIARSLSLPWEALFRSVEEATDEQ
                     VALLALQPDPQKHELSISGEAKDYAAILAFVARLDKRQALRNVHLVRHELREDDPQQP
                     MYFNIVANWESGS"
     gene            6122..6637
                     /locus_tag="VFV55_11020"
     CDS             6122..6637
                     /locus_tag="VFV55_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944875.1"
                     /translation="MKRLRWIIERSPLGWPGAVALVLVAVAVLGQVTVVPAMQARLDA
                     LTHPVHRAGASATNRDDDPANRLDRFYRHFREAGPAPDQLAKIYRIAATNGIALRQGD
                     YQLVAADGRLQSYHVTLPVAGPYPAIRRFLAETLDEIPTASLDQVVFERKRIGERGIE
                     AQLRLTLYLDR"
     gene            6637..7155
                     /locus_tag="VFV55_11025"
     CDS             6637..7155
                     /locus_tag="VFV55_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944876.1"
                     /translation="MRLPERPLPLIVLSAIALALAIGHAAGGDPNAGSEPVAEPVARD
                     HTAPGAQQVQPQLALALSRPRRAAGEDAVTDLFSSRSWVPAPPPAPPVPPAPPPEPTA
                     PALPFAYVGRLEADDAKPIIYLSRGERLLAVSEGDVVDKDYRIESLTNNEIVLVYLPL
                     GKQQTLRIGSER"
     gene            7155..9437
                     /locus_tag="VFV55_11030"
     CDS             7155..9437
                     /locus_tag="VFV55_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEX4944877.1"
                     /translation="MKSPLPRLNAVLATAVLAGAFVAGCASDRQHSEALALIEQQRYE
                     EGFALLDKNARESSEKGPMRAELLVRRDRAVLKLVEAGRSELNAGRLPEAQALFERAL
                     AVQPGNTQGREGLAAVDSARRRAETLQQARDKAKAGDTEGARAALALVLRDSRDNREA
                     LELQRQLDERDNRASIGTPALRVKNPRPVNLEFRDANLKMVFDALSRSSGVNFVFDKD
                     VRGDAKTTIYARQVTLEDAIDLILLTNQLEKKVIAENALLIYPSTQQKLRDYQDLVIR
                     SFYLENADVKTTFNMIKTMLKTRDAFVDEKLNLIVMRDTPEAIRLAEKLIAAHDLAEP
                     EVLLEVEVIEVGYSRLRELGVKWTDKFTFGLVDPTGGSLLLNELRGINSNRVTVSSPS
                     ATLSLGDTDGQSNVLASPRVRVKNRDKARIMIGDRVPVISSQITPSTGNPVVTENVSY
                     LDVGIKLDVEPTVHLDGDVSIKIGLEVSNLGERTTTQNGTIAYRVGTRNASTSLRLRD
                     GETQVLMGLIRDDDRRAASKVPGIAELPVLSHLFGSHTTESTKSEIVLSITPRIVRNV
                     RRVEARVAEFWSGTETNLRSQPQYARTIATAAAPGAPEAAGAAGAPAAGSAAALPPSG
                     IQLLWQGPAEAKVGDEVTLVLKARTAETLVSAAVQVGFDASTLEFVRITEGEFMKQGG
                     VATAMNHRLDAGAGTLNVRFARAGGGATGEGNLASLTFRVKDGAQTAKVQTLAVSPVG
                     PGNQPIALAAPAPHQVAVSR"
     gene            9448..9918
                     /locus_tag="VFV55_11035"
     CDS             9448..9918
                     /locus_tag="VFV55_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX4944878.1"
                     /translation="MRQRGFTLVELMVTVAIIGVLASVALPVAQLSLQRGKEQDLRVA
                     LRQVRDAIDAYKLASDEGRITRYAGKSGFPPSLEVLVNGVEDAKSPVKAKIYFLRRLP
                     RDPFATDPATPPALTWGKRSYSSPPDNPQEGEDVFDVYSLSAATGLNGLPYRSW"
     gene            9918..10298
                     /locus_tag="VFV55_11040"
     CDS             9918..10298
                     /locus_tag="VFV55_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4944879.1"
                     /translation="MDRLFSRGFTLIELLVVMAIISTLLALAAPRYFNSQDKARETAL
                     RHDLAVMREAIDRHYGDTGRYPESLQELVARKYLRRIPPDPITGSDETWIFASQANKN
                     KGAIVDVASGAPGVSREGTPYAQW"
     gene            10307..10816
                     /locus_tag="VFV55_11045"
     CDS             10307..10816
                     /locus_tag="VFV55_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX4944880.1"
                     /translation="MAARREAGFTYFTVLFLVTVMGLAAAAAGTMWHTVRQREKEREL
                     LALGEEFRAAIARYVRATPSGKPQYPRRLDDLVRDPRVPGMARHLRRIYPDPMTGRPD
                     WGVVPAPDGGIMGVHSLSEHQPLKRAGFRVADARFEGAEKYADWQFVYRDRFIGGTAA
                     PPNARRPGP"
     gene            11088..11462
                     /locus_tag="VFV55_11050"
     CDS             11088..11462
                     /locus_tag="VFV55_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944881.1"
                     /translation="MPRSHPKFRAVLVLTAGLLAAGTVSAATVSECVGDVSVAKADIT
                     AATTFVNAKDQTGLAGKADSAMQKLDKGKFADASLILADMAAKVTSLVSATKPKLGAE
                     DGALISADIGVASTCVTQLLTQ"
BASE COUNT         1835 a         4205 c         3889 g         1669 t
ORIGIN      
        1 acgcttgccg acctgctcct ctcgcagggc cgccacgagg aggcgctggc ggtgctgaag
       61 gacctctccg ggcgcgacgc gaggaacctg cgcctgctgc gcatgcggct cgatgccgaa
      121 acggcgctgc gtcgctggga cgaagtcctc gccaccgcct ccgcgctcgc caagctgggc
      181 gggctctccg cgcaggaagc cgccgcgtct cgccgtgccg cgcacctggg caacctcaag
      241 cgcaaggcaa cggacgccgc cgcgctcgcg gcgtactgga agcagattcc gtcggagctt
      301 cgcgtcgact ccgccatcgc cgccaccgcc gcgagctacc acctttcgct cggcggcgca
      361 gccgaggcgc agggcatcgt cgaatcggcg ctggaggcgg gctgggacgc gaggctcgtg
      421 gcgctctatg ccgactgcgc cggcgagtcc gcgctgccgc tgatcgagcg cgccgagaaa
      481 tggctgcgcg caaacgcgcg cgatccggca ctcctgctca cgctcggcaa gctctgcatg
      541 caccagggcc tgtggggcaa ggcgcagagc tacatcgagg cgagcctcgc cctcgagccc
      601 acgcacgagg gccacatgac gctcgccgcg ctgatggaaa ggctcggcaa gccccaggag
      661 gccgtccgcc attttcgccg cagcgcggaa ctcgcaggct agcggccccc tccaatccgt
      721 ttccgcaata cgcaaaggac ccggtcatga cgcgcgaata cttcgttctc cagcaccacg
      781 ccctggcgcc cggcgcgcga gtcggcacac cactgccgcc cgcccgcctc acgcccgagt
      841 cgccggcgct cgaggccatg accgacctgc agcggatcga ggccgccacc atcgcgcccc
      901 atcgcagcat cgtggagtgc aacacggtga tgatcgtgca ctcgatccgc ctgcttttcg
      961 tcgaggatgc ggaccgatcc gtggtcggcg tcatcaccgc gaccgacctg ctgggcgaga
     1021 agccggtgcg cttcatgcgg gagcggggcg tgcgccatca cgagatcctc gtgtcggacc
     1081 tgatgacgcc cgcttccgcg ctcgaggcgc tggacctgca gagcgtcacc accgcgcagg
     1141 tcggccacat cgtcgcgacc ctgaagtccg tcgggcgcca gcacaccttc gtgaccgacg
     1201 agggcggcaa gcgcatctgc ggcctgttct cggccacgga catcgcgcgc cggctcgggg
     1261 ccgaggtgcc gacgcacgag gtcgcggcca gcttcgcgca gatcgaggcc gccctgtcgc
     1321 gatgaccgac ggccacgcgg aggctgttcg cgccttccgc tggcaggtgc ccgcgcgctg
     1381 caacatcgcc gattccgcgt gccggcgctg ggccgcggac cgcgcgcgct tcgcgctcta
     1441 ctgggaagac gagtcggggg cgaccgccgc cttcacctac tgggatctgc tgcaggctgc
     1501 caaccggctg tcgaacgcgc tcgtggcatt gggggtggcg cgcggcgatc gcgtggcgat
     1561 cctgctgccg cagcgacccg agaccgtcgt cgcctacctc gcgtgcttcc agatgggcgc
     1621 gatcgccgtg ccgctgtcct tccttttcgg gcccgacgcg ctcgaatacc gcctcgccga
     1681 cagcgcggcg aaggtcgcga tcgccgatcc ggaaacgctg cccaacctgt gggcggtgcg
     1741 cgagcgcctt cccgagctgg cgcacgtgat cggcgtcgcc ggcgcgcgcg aatccggcgt
     1801 gcacgcgtgg gagtcgctcc tcgaacgcgc ctcgcggcgg ttcgactgcg tcgaaaccgg
     1861 cccgcgcgac cccgccgtca tcatctacac cagcggcacg accggcccgc ccaagggcgc
     1921 gctcatgccg caatcgacgc tcatcgggaa tctcccgggc ttcgagtact cgcacgacgg
     1981 cttcccgcag gagggcgatc tcttctggtc gccggccgac tgggcatgga cgggcggcct
     2041 gtgggacgcg ctcatgccca cgctgcacca cgggcaggcg atcctcggct accgcggccg
     2101 gttcgacccc gagaaggcgt tccatcttct cgacaagtac gcggtgcgca acgcgttcct
     2161 ttttccgacg gccctcaaga tgatgaggaa ggccgtcgac cacccgcgcg cgcggttcga
     2221 cctcgacctg cgctcgatga tgagcggcgg cgagcccgtg ggccccgcgc tcttcgaatg
     2281 gtgccgcgac gaactgggcg tcaccgtgaa cgagatcttc gggcagacgg agatgaacta
     2341 catcgtcggc aattcgcacc gcgtgtggcc ggtgaagccg ggctccatgg ggcgccccta
     2401 cccgggacat cgcatcgcgg tgatcggcga cgacgggctc gaggtgcccg cgggcacggt
     2461 gggcgacgtg gcggtgcatc gcgtcggcac cgacggcacg ccggaccccg tcttcttcct
     2521 cgggtactgg aacaaccccg aggcgacggc gaagaagtac accggcgact ggtgccgcac
     2581 gggcgaccac gccagcgcgg acgccgatgg ctacctctgg taccagggcc ggggcgacga
     2641 catgttcaag agcgcgggct accggatcgg gcccgcggaa atcgagaact gcctggtgaa
     2701 gcacgaggcc gtcgccaacg ccgccgtggt gccgagcccc gacgagacgc gtggcagcgt
     2761 ggtgaaggcg ttcgtgctcc tcgccccggg ccggacgcct tccccggcgc tcgaggaatc
     2821 gctgcgtgcg cacgtgaagg cccacctcgc gccctggcag cagccgcgcg agatcgagtt
     2881 cctggcggag ctgccgatga ccaccaccgg caaggtgcag cggcgcgtcc tgcgcgagcg
     2941 cgaggcgctg aaaaagcgcg gcggcacccc ctgagcgacc cccgcgccgc aaggtgcggt
     3001 ttattgtcgc agcacgtatt ttccgggcgg tttgggctct gcggcggggt acgattggtt
     3061 ccgatacttt gccgatggcg gggcgcgtcg cgtcccgccg tcgaagccat tgccgaacgg
     3121 agcaagaaac ttccatgccg ctcgaccaga tttcctcttc agccgcgatc accgccgcgg
     3181 acatcgcggc cgcgcgcgcg gaatcggcgc agaccggcca gccgatgatc gacgtgctcg
     3241 agcgccagtc cggcctctcc ggcgacgact tcgtcgcggc gctcggccgc gcgctcgcct
     3301 ttcccgccgt ccgcatggac gagctgcgcg aaggtgatgc cgcgttcgac gtggtgccgt
     3361 ttcccgaggc ggtcgaacgc ggctgcgtgc cgatccggcg caacggcgcg ctggcgctcg
     3421 tccacggtga tccgttccgc gccggcacgc gggcctggat ggaggagcgc gtcggcgtcc
     3481 cgttctcgtg gctgctcgcg catccctcca ccatcgcggc attcttccgg cgtttcgagg
     3541 agtccgcccg ggcgatggac gacgtcgtgc agtacgccac cgatcccgag gaaggcggcg
     3601 cgcagcgcgg cgcgaccctg tcgctgcaat cgatcggcga ggacgagagc ctcgcggtgc
     3661 gcctggtgaa ctcgacgctc tacgacgcgc tcaagtccgg cgcgagcgac atccacctgg
     3721 agaccgatcc gggcggcctt tcggtcaagt accgggtcga cggcgtcctg tccgccgtgg
     3781 ccaagccgcg cggcatcgac ttcgccgagc aggtgatctc gcgggtgaag gtgatggccg
     3841 agctggacat cgcggagcgc cgcgtcccgc aggacggccg tttccgcatc gtctcccagg
     3901 ggcgcgccgt ggacctgcgc gtgtcggtga tgccgagcat ccacggcgag gacgcggtgc
     3961 tgcgcatcct cgacaagcag tcgctcgccg acgagatacg cggcctgcgg ctcgaccggc
     4021 tgggcttcga cccggacatc gtgaatgcca cgctcgcgct cgcgtcgctt ccctacggca
     4081 tgctcctggt gaccgggcct acgggcagcg gcaagacgac cacgctctat gcagccgtca
     4141 ccgagatcaa caccggacag gacaagatcg tcaccatcga ggatcccgtg gagtaccagc
     4201 ttcccggcgt gctgcagatc ccggtgaacg agaagaaggg gctcaccttc gcgcgcgggc
     4261 tgcgctcgat cctgcgccac gacccggaca agatcctggt gggcgagatc cgcgacccgg
     4321 acaccgcgca gatcgccgtc caggcagcgc tcaccggcca cctcgtgctc accacgatcc
     4381 acgccaacag cgccttcgac gtgatcgggc gcgtgggaaa catgggcgtc gacgcgttca
     4441 gcttcgcgtc ggccacgaac gggatcctcg cgcagcgcct cgtgcgcgtg acctgcgaac
     4501 attgcgccga gccgcagacg cacccggagg aactgctgcg cgcctcggga atcgaccccg
     4561 ccgatgcggg gcgctacctc ttccgggccg ggcgcggctg cggccattgc cggggctcgg
     4621 gcttccgcgg ccgccgcgcc atcgccgagc tgcttcacct cgacgaggag atccgcgagc
     4681 tgatcgcgcg ccgcgagtcg atccgtgcga tccgccaggc ggcgcgcgcc aacggcacgc
     4741 ggatgcttcg cgaatcggcg ctcgatctcg tcctgcgcgg cgagacgacc ctccaggaga
     4801 tcaatcgtgt tactgccgtg gcgtgacgaa ctgcacatcg ccattcgccc caccgagctg
     4861 gttcgcgtac ggcggacctt cggcgcgcgc ggcgttcgcg cggagcatgc cacgtcaccc
     4921 gtcgagccgg cagccgacaa ccgtgcctgg cgcagcgcga tcgacgtcct cgcgcagggc
     4981 gtcgcgcagc tcgcggagcg ccccgcgagc gtgcggatcg tgctctcgaa tcatttcgtt
     5041 cgctatgcca tcgcgccgtg gcgggacgac atgcgctctc ccgcggaacg cgagggtttc
     5101 atccgccact gcttccgcga aaactacggc gacgcggccg aaggctgggt gatccgggag
     5161 aacccggaac cctacggccg ggcttccctc gcctgcgccg tcgaccgtga gctggtcgat
     5221 gcggtgcgcg atgtcttccg cggcacgcgg ctgagcctgg ctgccatcca gccgttgttc
     5281 atggccgcgt tcaacaagta ccgcaaggcg ctcgggtcga ccggctgctt cctggtctac
     5341 gaaaagggca ggctctgcgg cgcgacctat gccgacggcg aatggcgaag cgcccttgcc
     5401 gtgcgcgtgg aggaaggttg cttcaacgca gcgacgatcg agcgtgagct cctgctgcaa
     5461 ggggtacccg aggattttcc ggtcttcctt tgccaggtcg acgacgcgtc cgatcccggc
     5521 acgttcgccc ggccggtgaa gcgcctcgat ccgccggggc tggacgagcc ggacttcacc
     5581 gccgccttgc tcgggggtgg cgagtgaaac gcctgcgcct cgacctggcc ggcccgcgcg
     5641 gctcctccgc cgggtggatc ctgctcgggc tggccctcgt ggccctcgcc gacctcggtg
     5701 catcctggcg tcagctggac gaggagatcg cggcggcgga acagcaggcg gcgcggccgc
     5761 gcgcgcgcgt cggcgaacac accgcgcgag gagctcgcgg cggcgagggc gcagccggtg
     5821 acttgcgcga cgccgatcgc atcgccagga gcctctcgct gccctgggag gcgcttttcc
     5881 gcagcgtcga ggaggcgacc gacgaacagg ttgcgctgct cgcgctgcag cccgacccgc
     5941 agaagcacga actgagcatc tccggcgagg ccaaggacta cgcggcgatc ctcgccttcg
     6001 tcgcccgcct cgacaagcgc caggcgcttc gcaacgtcca cctcgtcagg cacgagctgc
     6061 gcgaggacga cccgcagcaa ccgatgtact tcaacatcgt ggccaactgg gagtccggct
     6121 cgtgaagcgc ctgcgctgga tcatcgagcg atcgccgctg ggctggcccg gcgcggtggc
     6181 gctggtcctg gtcgccgtcg cggtcctcgg gcaggtcacg gtcgtgccgg cgatgcaggc
     6241 ccggctcgac gccctgaccc atcccgtgca ccgggcgggc gcatcggcga cgaatcgcga
     6301 cgacgacccg gcgaaccgcc tcgaccgctt ctaccggcat ttccgcgagg cgggtcccgc
     6361 gccggaccaa ctcgcgaaga tctaccgcat cgccgccacg aacggcatcg cgctccgcca
     6421 gggcgactac cagctcgtgg ccgccgacgg ccgcctgcag agctaccacg tcaccttgcc
     6481 cgtcgcgggc ccgtatccgg ccatccgtcg cttcctggcg gaaacgctcg acgagattcc
     6541 caccgcctcg ctcgaccagg tcgtcttcga gcgcaagcgc atcggcgagc gcggcatcga
     6601 ggcgcagttg cgcctcaccc tctacctgga ccgatgatgc gcctgcccga acgcccgctc
     6661 ccgctcatcg tcctctcggc gatcgccctc gccctggcga tcgggcacgc cgcgggcggc
     6721 gacccgaatg ccggcagcga acccgtcgcg gagccggtcg cgcgcgatca caccgccccc
     6781 ggggcgcaac aggtccagcc gcaattggcg ctggcgctca gccgtccgcg gcgcgcggcg
     6841 ggcgaggatg ccgtgaccga cctcttctcc agccggtcgt gggttcctgc gccaccgcct
     6901 gcgccgcccg tcccgcccgc gccgccgccc gagccgaccg cacccgccct gcccttcgcc
     6961 tacgtggggc gcctggaggc cgacgacgcg aagccgatca tctacctttc gcgcggcgaa
     7021 cgcctgctgg cggtaagcga gggggacgtc gtggacaagg actaccgcat cgaatccctc
     7081 accaacaacg aaatcgtgct ggtctacctg cccctcggca agcaacagac ccttcgaatc
     7141 gggagcgaac gctgatgaaa tcccccttgc cgcgcttgaa tgcggtcctc gccacggccg
     7201 tactggcggg cgcattcgtc gccggctgcg ccagtgaccg gcagcacagc gaagcgctcg
     7261 ccctcatcga gcagcagcgc tacgaggaag gcttcgccct cctcgacaag aacgcgcgcg
     7321 aatcgtccga aaagggaccg atgcgcgccg aactgctcgt gcggcgcgac cgcgccgtgc
     7381 tgaagctggt cgaggccggc cgcagcgagc tcaatgccgg gcgcctgccg gaggcgcagg
     7441 cgctgttcga gcgcgcgctc gccgtccagc cgggcaacac ccagggtcgc gaagggctgg
     7501 cggccgtcga cagcgcgcgc cgccgggccg agaccctgca gcaggcgcgc gacaaggcca
     7561 aggcgggcga cacggaaggc gcgcgggccg ctctcgccct cgtgctgcgc gacagccgcg
     7621 acaaccgcga ggcgctcgag ctgcagcgcc agctcgacga acgcgacaac cgcgcgtcga
     7681 tcggcacgcc cgcgctgcgc gtgaagaacc cgcgcccggt gaacctcgag tttcgcgacg
     7741 ccaacctcaa gatggtcttc gacgcgctgt cgcgttcgag cggcgtgaac ttcgtcttcg
     7801 acaaggacgt gcgcggcgac gcgaagacca ccatctacgc gcggcaggtg acgctcgagg
     7861 acgcgatcga cctcatcctg ctcaccaacc agctggagaa gaaggtcatc gccgaaaacg
     7921 ccctgctcat ctacccgagc acccagcaga agctgcgcga ctaccaggac ctcgtcatcc
     7981 gcagcttcta cctggaaaac gccgacgtga agaccacctt caacatgatc aagacgatgc
     8041 tcaagacgcg cgacgcgttc gtcgacgaga agctgaacct gatcgtgatg cgcgacaccc
     8101 cggaagcgat caggctcgcc gagaaactca tcgccgccca cgaccttgcc gagccggaag
     8161 tgctgctcga ggtcgaggtg atcgaggtcg ggtattcgcg gctgcgcgag ctgggcgtca
     8221 agtggaccga caagttcacc ttcggcctcg tcgatcccac cgggggaagc ctcctcctga
     8281 acgagctgcg cgggatcaac tcgaaccggg tcacggtcag ctccccctcg gccaccctga
     8341 gcctgggcga caccgacggg cagagcaacg tgctcgcctc gccgcgggtg cgcgtgaaga
     8401 accgcgacaa ggcgcgcatc atgatcggcg accgcgtgcc ggtgatctcg agccagatca
     8461 cgccttcgac cggcaacccc gtcgtgaccg agaacgtctc ctacctcgac gtgggcatca
     8521 agctcgacgt cgagcccacc gtgcacctcg acggcgacgt gtcgatcaag atcggcctcg
     8581 aggtgagcaa cctcggcgag cgcacgacca cgcagaacgg gacgatcgcc taccgcgtcg
     8641 gcacgcgaaa cgcgtccacg tcgctgcgcc tgcgcgacgg cgagacccag gtcctgatgg
     8701 gcctcatccg cgatgacgac cgccgcgccg catcgaaggt gcccggcatc gccgaactcc
     8761 cggtgctgag ccacctcttc ggcagccaca cgacggaatc gaccaagtcg gagatcgtgc
     8821 tctccatcac gccgcgcatc gtgcgcaacg tccggcgcgt ggaggcacgc gttgccgagt
     8881 tctggtccgg aacggagacg aaccttcgct cgcagccgca atacgcgcgc accattgcca
     8941 cggcggccgc gccgggcgcc cccgaggccg cgggtgccgc gggcgctccc gccgccggaa
     9001 gcgccgcagc cctgccgcct tccggcatcc agctcctctg gcaggggccc gccgaggcga
     9061 aggtcggcga tgaggtgacg ctcgtgctga aggcgaggac ggccgagacg ctggtgagcg
     9121 ccgccgtgca ggtcggattc gacgcctcca cgctcgaatt cgtgcgcatc accgaagggg
     9181 agttcatgaa gcagggaggg gtcgccaccg cgatgaacca ccgcctggac gcgggcgcag
     9241 gcacgctcaa cgtgcgcttc gcccgcgccg gcggtggggc gactggcgag ggcaacctcg
     9301 cctcgctcac cttccgcgtg aaggacgggg cacagaccgc gaaagtccag acgctggccg
     9361 tctcgcccgt cgggccgggc aaccagccca ttgccctcgc cgctcccgcg ccccaccagg
     9421 tcgcggtttc ccgctgagga tcgagacatg aggcagcgtg gcttcaccct cgtcgagctg
     9481 atggtcacgg tggccatcat cggtgtgctc gcgagcgtgg cgctgcccgt ggcgcagctc
     9541 tcgctgcagc gcggcaagga gcaggacctg cgcgtggcgc tgcgccaggt tcgcgacgcg
     9601 atcgacgcct acaagctcgc gtccgacgag gggcgcatca cccggtacgc cggcaagtcg
     9661 ggctttcctc cgtcgctgga ggtgctcgtg aacggcgtcg aggacgcgaa gagccccgtc
     9721 aaggcgaaga tctacttcct gcgccggctg ccgcgcgacc cgttcgccac cgaccccgcc
     9781 acgccccccg ccctcacctg gggcaagcgc agctattcga gcccgccgga caatccgcag
     9841 gagggcgagg acgtcttcga cgtgtactcg ctttccgcgg caaccggcct gaacggcctg
     9901 ccctacagga gctggtaatg gatcgcctct tctcgcgcgg attcacgctc atcgaactgc
     9961 tcgtcgtgat ggcgatcatc tcgacgctgc tggcgctcgc cgcgccgcgc tatttcaaca
    10021 gccaggacaa ggcgcgcgag acggcgctgc gccacgacct ggcggtcatg cgcgaggcga
    10081 tcgatcgcca ttacggcgat accggccgct acccggagtc gctgcaggaa ctcgtcgccc
    10141 ggaagtacct gcgccgcatc ccgccggacc cgatcaccgg cagcgacgag acgtggatct
    10201 tcgcgagcca ggccaacaag aacaagggcg ccatcgtcga tgtcgccagc ggcgcgcccg
    10261 gcgtgagccg cgagggcacg ccgtacgcac agtggtaggc aacgccatgg ccgcgcggcg
    10321 cgaagcgggc ttcacctact tcaccgtgct cttcctggta acggtcatgg gcctcgccgc
    10381 cgccgctgcg ggaacgatgt ggcacaccgt ccggcagcgc gagaaggaac gcgaactgct
    10441 cgccctgggc gaggagtttc gtgccgccat cgcgcgctac gtgcgcgcca cgccgagcgg
    10501 caagccgcag tacccgcggc ggctcgacga cctcgtgcgc gatccgcgcg ttcccggcat
    10561 ggcgaggcac ctgcgccgca tctacccgga cccgatgacc ggccggccgg attggggagt
    10621 cgtcccggca ccggacggcg gcatcatggg cgtccacagc ctctccgagc accagcccct
    10681 caagcgcgcg gggttccggg tggccgatgc gcgtttcgaa ggggcggaaa agtacgccga
    10741 ctggcagttc gtctaccgcg accgcttcat cggcggcacg gcagcgcctc cgaacgcgcg
    10801 gcgaccgggc ccgtaggccc ctgcccggaa gtggggtgcg acaataaacc gcgcccagtt
    10861 gtcttgccta tgcaattcgc tccgctacac tcgtagctca ggctccgcgc gggacatcgc
    10921 agccaggcga tgctcgggaa tatcggatct ccccggcgca ggggggaggg atacaaccgc
    10981 caggtttgtg gtttcgaaag gccgggcgag gggacccgcg ccacgcttcc cccgttccgc
    11041 cggaaatgcc tacccaacta ttagaccttc accataggag tccaaaaatg ccccgttccc
    11101 atccgaaatt ccgtgctgtt ctcgtcctga ccgcaggttt actggccgca ggtacggtca
    11161 gcgcagccac cgtctccgaa tgcgtcggcg acgtctcggt ggccaaggcc gatatcacgg
    11221 ctgccaccac cttcgtgaac gccaaggacc agaccggcct tgccggcaag gccgacagcg
    11281 ccatgcagaa gctcgacaag ggcaagttcg ccgacgcgtc gctgattctt gccgacatgg
    11341 cggccaaggt gacctccctc gtgagcgcca cgaagccgaa gctgggcgcc gaggatggcg
    11401 cattgatctc ggcggacatc ggcgttgcga gcacttgcgt gacgcaactc ctgacgcagt
    11461 gacgtgaacg cccgccgcgc tgcgatttag ctcgccgcgg caccactcag caatgaaccg
    11521 gggaggccat ggcctccccg gttcattgct gagtggtgcc gcggcgagct aaatcgcagc
    11581 gcggcgggcg ttcacgtc
//
LOCUS       DASUEV010000350         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_797336, whole genome shotgun sequence.
ACCESSION   DASUEV010000350 DASUEV010000000
VERSION     DASUEV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797336"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..1105)
                     /gene="phbB"
                     /locus_tag="VFV55_11055"
     CDS             complement(362..1105)
                     /gene="phbB"
                     /locus_tag="VFV55_11055"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX4944882.1"
                     /translation="MKQRIALVTGGMGGLGEAICMKLARMGIKVVVTYSPGNTKYKDW
                     LKDMEGRDYHFHAYPCDVADFDSCQAAATTITAEVGPIDILINNAGITRDMTFKKMGK
                     VDWDAVIRTNLDSVFNMTKPVVDAMVERGWGRVINVSSVNGQKGAFGQTNYSAAKAGM
                     HGFTKALALEVARKGVTVNTISPGYIGTQMVMAIPKEVLDTKIVPQIPIGRLGKPEEV
                     AGLCAYLASDEAAFITGANISINGGQHMF"
     gene            complement(1168..2907)
                     /gene="phaC"
                     /locus_tag="VFV55_11060"
     CDS             complement(1168..2907)
                     /gene="phaC"
                     /locus_tag="VFV55_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX4944883.1"
                     /translation="MPKSHSPQAERIAGILRKSHEKMASSMHADSPTHHVMAGFSEAY
                     RAWLESVTAKPQTLLDLQGKYMQEQMNLWMKAFQPDGEGEERPGADKRFSAPEWNELP
                     IFRYYRDSYLLTSKMMMQAVEEADMDAATKRRMRFFMRQYLDAAAPSNYLATNPEAIK
                     AALESGGKSIEEGMKNLMADMEKGRISMTDEAAFEVGKNIAVSKGAVVFENDLIQLIQ
                     YSPLTDKVHERPLVMVPPCINKFYIMDLQPENSLVRYAVEQGHTVFMVSWRNVKPPLD
                     KLTWDDYVEMGVIAALDEARAVSGADKVNALGFCIGGTLLSCALAVLAKKRRSPVASL
                     TLMTALLEFTEVGEIRVYIDKNFVEKREKKLAQGGIVPGAELASAFSSLRANDLVWTY
                     VVSNYLKGKQPPAFDLLYWNGDSTNLPGPMYAYYLRNTYQDNKLVQPDALTMCGVPLD
                     LKRVEVPAFVFAAREDHIVPWKGGYESARYLGGPVKFVLGASGHIAGSINPAAKGKRS
                     YWTHDRLGTDPDKWFAQATEVPGSWWVPWTKWLKPFAGKLVAARTRLGDAKHKPIEPA
                     PGRYVKERAEIEK"
     gene            complement(3018..3830)
                     /locus_tag="VFV55_11065"
     CDS             complement(3018..3830)
                     /locus_tag="VFV55_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX4944884.1"
                     /translation="MPVLAWILAACLAGGLLSVLLAAVVAFRVQARLVPILVSYAVGA
                     LLGAVFLDMLPHLFEQSADVGATAALILFGILGFFVLEKLLLWRHDHGDAGETAASGP
                     ALTAHGHAHGHAHGADGGRSGWMIIVGDSFHNFTDGVIIASAFLADVKLGVVTSAAIV
                     AHEVPQEVGDFLVLLNSGFSKARALALNAFSSLAAVLGALLGYFALSSAQQWVPRILA
                     ISAAGMLYVAVADLIPGLQRRTRFAESVAQVAFIALGILTLWVVHLALGQGH"
     gene            complement(3882..4631)
                     /gene="pgeF"
                     /locus_tag="VFV55_11070"
     CDS             complement(3882..4631)
                     /gene="pgeF"
                     /locus_tag="VFV55_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX4944885.1"
                     /translation="MKLPAGWIVPDWPAPARVRAFVTTRAGGVSEGEYGSLNLGMKSG
                     DDPARVAANRLAVRAHLPSSPHWLAQVHGNTAADLDMAGASGMTVADAAATSVAGRVA
                     VVLTADCLPVFLCREDGSRVAVAHAGWRGLAAGVLENAVIALGGEASRVLAWLGPAIG
                     PAAFEVGPEVREAFVSAHGEDASAFTRRGEHKFMADLYALARLRLARAGVGGVSGGGF
                     CTWTESRRFFSYRRVRESGRMGAFIWIDEGR"
     gene            complement(4628..>5606)
                     /locus_tag="VFV55_11075"
     CDS             complement(4628..>5606)
                     /locus_tag="VFV55_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX4944886.1"
                     /translation="RSTAVKLRFMIDPVAKPANYTSREGPAPKLALSVEPALAGLRLD
                     QALARLRPKESRSRLARLVEEGAVLVDGERVRPRHKLRGGEQVEVTLVPRPEESAFQP
                     EEIALDVVHEDDDVLVVAKPAGLVVHPGSGNWAGTMLNALLHRVPALKGLPRAGIVHR
                     LDKETSGLLVVAKHEAAMQDLVRQLQARTVKRTYLAIARGVMAGEGTVDAPIGRHPKQ
                     RTRMAVVPGGKPAVTHYRVRERFGAHALLECDLETGRTHQIRVHLASIGHPLEGDPVY
                     GARGARRLPRQALHAWKLAFIHPGTGKMVRFTAPPPEDFEALVEALRSE"
BASE COUNT          862 a         1902 c         1897 g          945 t
ORIGIN      
        1 gtcgggcccg ggatggggca agtcaatcgg cgtcctgggc acggcgaagg ggagtcgggc
       61 cggggatggg gcaagtcaat cggcgtcctg ggcacgcaaa cgtgaccgtc ggatgggtgc
      121 ccggaaagtc gaggccagcc aaacacgtct ggcctcgact ttccacccat ctagagacga
      181 gcgcgctaac gctttctcgt ccgcgacggt cgcgtgccaa ggattgcgat cgacttccac
      241 cgccaccgcc tcccacgctc cgtggatccc gtgtcgagtg aggaaacgtc ggaccgcgct
      301 cgtccgctgt cgaaagcgcc acgacgcctc ttcaagtggg ccgggcacgc gtgccagcgc
      361 tctagaacat gtgctgcccg ccgttgatcg agatgttggc gccggtgatg aacgccgcct
      421 cgtcggaggc gagatacgcg cacaggcccg ccacctcctc gggcttgccc aggcgcccga
      481 tcgggatctg cggcacgatc ttcgtgtcca acacttcctt ggggatcgcc atcaccatct
      541 gggtcccgat gtagcccggg gagatggtgt tcacggtgac gcccttcctg gcgacttcca
      601 gcgcgagagc cttggtgaag ccgtgcatgc ccgccttggc ggccgagtag ttggtctggc
      661 cgaaggcgcc cttctgcccg ttcacgctcg agacgttgat cacgcgcccc cagccgcgct
      721 cgaccatcgc atcgacgacg ggcttggtca tgttgaagac gctgtcgagg ttggtcctga
      781 tcaccgcgtc ccagtccacc ttgcccatct tcttgaaggt catgtcgcgc gtgatgcccg
      841 cgttgttgat gaggatgtcg atggggccga cttccgccgt aatcgtggtc gccgccgcct
      901 ggcacgagtc gaagtcggcc acgtcgcagg gataggcgtg gaagtggtag tcgcggcctt
      961 ccatgtcctt cagccagtcc ttgtacttcg tgttgccggg cgagtaggtc accacgacct
     1021 tgatgcccat gcgcgcaagc ttcatgcaga tggcttctcc gaggccgccc atgcctccgg
     1081 tcacgagggc gatgcgttgc ttcatgtcgt ttctcctcca ggcggattgt tatttcgcgc
     1141 cgcgggcggc cccgcgccgc gcgtgggtca cttctcgatc tcggcccgct ccttcacgta
     1201 gcgccccggc gccggctcga tgggcttgtg cttcgcgtcg ccgagcctgg tgcgcgcggc
     1261 cacgagtttg ccggcaaagg gcttcaacca cttggtccac ggcacccacc agctgccggg
     1321 cacttccgtc gcctgcgcga accacttgtc gggatcggtg ccgaggcggt cgtgggtcca
     1381 gtagctcctc ttgcccttgg ccgcgggatt gatcgaaccc gcgatgtgtc cgcttgcccc
     1441 gaggacgaat ttcaccggcc cccccaggta acgcgcgctc tcgtagcctc ccttccacgg
     1501 cacgatgtgg tcctcgcgcg cggcgaacac gaaagcgggc acctcgacgc gcttcaggtc
     1561 caggggcacg ccgcacatgg tgagcgcgtc gggctggacg agcttgttgt cctggtaggt
     1621 gttcctcagg tagtacgcgt acatcgggcc ggggaggttg gtcgagtccc cgttccagta
     1681 gagaagatcg aacgccggcg gctgcttgcc cttcaggtag ttgctcacga cgtaggtcca
     1741 gaccaggtcg ttcgcgcgca ggctcgagaa ggcgctggcg agctccgccc cgggcacgat
     1801 gccgccctgc gcgagcttct tctcgcgctt ctcgacgaag ttcttgtcga tgtagacgcg
     1861 gatctcgccc acctcggtga actcgagcag cgcggtcatg agcgtgaggc tcgccaccgg
     1921 gctccggcgc ttcttcgcga ggaccgcgag cgcgcaggaa aggagcgtgc cgccgatgca
     1981 gaagccgagc gcgttcacct tgtccgcgcc gctgacggcg cgcgcctcgt cgagcgcggc
     2041 aatcacgccc atctcgacgt agtcgtccca ggtcagcttg tccagcgggg gcttcacgtt
     2101 tcgccacgac accatgaaca ccgtgtgccc ctgctcgacc gcgtagcgca cgagcgaatt
     2161 ctcgggctgc aggtccatga tgtagaactt gttgatgcag ggcggcacca tcaccagcgg
     2221 gcgctcgtgc accttgtcgg tgagcgggct gtactggatg agctggatca ggtcgttctc
     2281 gaagacgacc gcgcccttgg aaacggcgat gttcttgccg acctcgaagg cggcctcgtc
     2341 ggtcatggag atgcggccct tctccatgtc ggccatgagg ttcttcatcc cctcctcgat
     2401 cgactttccg cccgactcga gcgccgcctt gatggcctcg gggttggtgg cgaggtagtt
     2461 cgacggcgcc gcggcgtcaa ggtactggcg catgaagaag cgcatgcgcc gcttggtggc
     2521 cgcgtccatg tcggcctcct cgaccgcctg catcatcatc ttcgaggtga gcaggtacga
     2581 gtcgcggtag tagcggaaga tcggcagctc gttccattcg ggggcgctga agcgcttgtc
     2641 ggcgcccgga cgctcctcgc cctcgccatc gggctggaag gccttcatcc agaggttcat
     2701 ctgctcctgc atgtactttc cctgcaggtc gagcagcgtc tgcggcttgg cggtgaccga
     2761 ttcgagccag gcgcgatagg cctcggaaaa gcccgccatc acgtgatgcg tcgggctgtc
     2821 ggcgtgcatc gaggaggcca tcttttcgtg cgacttgcgc aggatgccgg cgatgcgttc
     2881 ggcttgcggg gagtgcgatt tgggcatggg ggaagtcgct ccgtgttacg acgcaaacct
     2941 ttggatattc agggattttc gccgatattg tggggggccg cttcggggag tgtcaaggaa
     3001 tccgcgcggg gtgcagccta atgtccttgg ccgagggcca agtgtacgac ccagagcgtg
     3061 aggataccca gggcgatgaa tgcgacttgc gccaccgatt cggcgaaccg cgtgcgccgc
     3121 tgcaggcccg ggatcaggtc cgccaccgcc acgtacagca tgcccgccgc ggagatcgcg
     3181 aggatgcgcg gcacccattg ctgcgcggag gatagcgcga agtaaccgag cagcgcgccg
     3241 agcaccgccg cgaggctcga gaacgcgttg agcgccagcg ccctcgcctt cgagaagccg
     3301 gaattgagca gcacgaggaa atcgcccact tcctgcggca cctcgtgcgc gacgatggcg
     3361 gccgaggtca ccacgcccag cttgacgtcg gcgaggaagg cgctcgcgat gatcacgccg
     3421 tcggtgaaat tgtggaacga gtcgccgacg atgatcatcc agccggagcg gccgccgtcc
     3481 gccccgtgcg cgtggccgtg tgcgtggccg tgcgcagtga gcgccgggcc gctcgccgcc
     3541 gtctctcccg cgtcgccgtg gtcgtgtcgc cagagcagca gcttctccag cacgaagaag
     3601 ccgaggatgc cgaagaggat gagcgccgcc gtggcgccga cgtcggccga ctgctcgaag
     3661 aggtgcggca acatgtcgag gaacaccgcg cccagcagcg cacccacggc gtaactcacc
     3721 aggatgggca ccaggcgcgc ctgcacccgg aacgcgacca cggccgcgag caggaccgag
     3781 agcagtcccc ccgcgaggca ggcggcgagg atccaggcga gtacgggcat ggttgggaga
     3841 gcggttcgcg cgactgagtt gcaacaagtc tagcagcact gtcaacgccc ttcgtcgatc
     3901 cagatgaagg cgcccatccg cccgctctcc cgcacgcgcc ggtaggagaa gaagcgccgc
     3961 gattccgtcc aggtgcagaa gccgccgccc gaaacgccgc ccacgcccgc tctcgccagg
     4021 cgcagtcgcg caagggcata gagatcggcc atgaacttgt gctcgccgcg gcgggtgaat
     4081 gcggatgcgt cctcgccgtg ggcggagacg aacgcctcgc ggacttcggg gcccacctcg
     4141 aaggcggccg ggccgatggc ggggcccagc cacgcgagca cgcgcgacgc ctcgccgccc
     4201 agcgcgatga cggcgttctc cagcacgccc gcggcaagac cgcgccaacc cgcgtgcgca
     4261 acggccacgc gcgatccgtc ctcgcggcag aggaacaccg gcaggcagtc ggcggtaagg
     4321 accacggcca cgcgccccgc gaccgaagtt gcggcggcat cggcgacggt catgccactc
     4381 gcgcctgcca tgtccaggtc ggccgccgtg ttgccgtgca cttgcgcaag ccagtgcggc
     4441 gatgacggca ggtgcgcgcg caccgcgagg cgattggcgg caacccgcgc cgggtcgtca
     4501 ccgcttttca tgccgagatt tagcgagccg tactcgccct cgctcacgcc gccggcgcgc
     4561 gtggtcacga aggcgcgcac gcgcgcgggt gcgggccagt cggggacgat ccatccggcg
     4621 ggcagcttca ttcgctgcgc agcgcctcca cgagcgcctc gaagtcctca ggcggcggcg
     4681 ccgtgaaacg caccatcttc ccggtgcccg ggtggatgaa ggcgagcttc catgcgtgca
     4741 gcgcctgccg cggcaggcgc ctcgcaccgc gtgcgccata gaccgggtcg ccctcgaggg
     4801 gatggccgat ggaggcgagg tgcacgcgga tctggtgcgt gcggcccgtc tccaggtcgc
     4861 attcgaggag cgcgtgagcg ccgaagcgtt cgcgcacacg gtaatgggtg acggcgggct
     4921 tgccgccggg caccaccgcc atgcgcgtgc gctgcttcgg gtggcgcccg atgggcgcgt
     4981 cgacggtgcc ttcgcccgcc atgaccccgc gcgcgatggc caggtaggtg cgcttcaccg
     5041 tgcgcgcctg caactggcgc accaggtcct gcatcgcggc ctcgtgcttc gccaccacca
     5101 gcagtccgct cgtttccttg tccagccgat gcacgattcc ggcgcgcggc agtcccttca
     5161 acgcgggcac ccggtgcagc agcgcgttca gcatcgtccc ggcccagttg ccgcttccgg
     5221 ggtgcacgac caggcccgcg ggcttggcga ccacgaggac gtcgtcgtct tcgtgcacca
     5281 cgtcgagcgc gatttcctcg ggctggaagg cggactcctc gggccgggga acgagcgtca
     5341 cctcgacctg ctcacccccg cgcagcttgt ggcgcggccg gacgcgctct ccgtccacga
     5401 gcaccgcacc ttcctccacg aggcgggcca ggcggctcct cgactccttt ggtcgcaggc
     5461 gcgccagcgc ctggtccagg cgcaaccccg ccagcgccgg ctccacggag agtgcgagct
     5521 tcggggcagg cccctcgcgg gaggtataat tcgcgggttt cgccacggga tcgatcatga
     5581 agcgaagttt aacggcagtg ctcctc
//
LOCUS       DASUEV010000351        10576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1703315, whole genome shotgun sequence.
ACCESSION   DASUEV010000351 DASUEV010000000
VERSION     DASUEV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703315"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..2686
                     /locus_tag="VFV55_11080"
     CDS             53..2686
                     /locus_tag="VFV55_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX4944887.1"
                     /translation="MSDTKFTRRTFLKVSAGTTAAAAAAPRLLHAMETELGGKDVSPV
                     TLAERKAIPINCHVCNIQDGAIAYVENGRVVKLEGNPEHVSTRGRLCAKGNSGMWYSY
                     DPDRILYPLKRVGARGEGKWKRITWDAALAEVAQKLDAALKEDPNTIMMKYGRNRTGG
                     TLDRFMQTLGSATVVNHTSVCESSKKIGMEPTWGPDIETPDFANTKYVLNIGSNILEA
                     AYFHNPLSQRITEGRVDNQMKIVTFDVRLSNTAGFSDEWIPVFPGTDGAVALALGHVI
                     LREDLQDSEFINTWTNVTVDELKAHYKDYTPEWASKISGVPAATIERIAREFALAKPA
                     TLFTYRGPAKHLYGSYNEKACMMLPIMTGNVETRGGYCLPRGMGWPQPDPKPPGAKNP
                     SYLSKPPDYPLAHHKVSHLTPFFIADGKQKINVYFTYQDNAVYTNPGAQAVWGKLFRD
                     EKLLPYIVSMSSHMGEETALADIILPDCPYLERWEPESMPNSLWPWLGIRQPVHASLG
                     ESRENRVLLRDIIHKLDPDGKRGMKQYWNFKDAEDYMRQHFDNVPGLKEAGGLDFLKK
                     HGVWPIYGKLDPKTGKVTDKTGREIKAEYGLHKKELSAAEMTGAVADKSGTITKDGKA
                     IGIKRNGRNYVGFPTGNRLINVRVEEWEKYGFNPMPTFKRIPWHEAMKEDEMILTTFK
                     LNVHKQSRTAAVKWLAEIAHSNPAWLNAQTAKKLGVKTGDLIRIESKVGHMVTKAYVT
                     EGIQPKVIAIATAFGHWEYGRLATLRLKDKPAFGGQEDADMSNVWWDDKGVHPNAIIP
                     VVADPIGGSQGWMDTVVKVTKAGPNDKYGDTQGNWEKHYEAYKETLRYAYTGDLHRKM
                     HPEMASWAGPDSVKPKSGGGH"
     gene            2762..3604
                     /locus_tag="VFV55_11085"
     CDS             2762..3604
                     /locus_tag="VFV55_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX4944888.1"
                     /translation="MLNELLLDDRPDPVPARAQVAPSAEDLRHECIARAGVYRLIAGV
                     FVEEPGVEFLATLRSRPALEALAEAGVRFESDFLDPDPATLAEALAVEYTTMFAASGG
                     FPPVESIRLTGRYQQAPHFDVKETYRRAGFVLSKGHFQVLEDHLGVELLFVAELLDRA
                     VAALDRGDLAGYRAVDRDIKRFWTLHLARWVRGYCHLVERASEHSLFREMARFLAGFA
                     TEEIESMRLRVDDLDQGREVVPKSEVQVAFNPDEPVCGACGNGDEPTLPRGAIPIAIR
                     PAAA"
     gene            3625..4065
                     /locus_tag="VFV55_11090"
     CDS             3625..4065
                     /locus_tag="VFV55_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944889.1"
                     /translation="MLVELKSRVILPPDRGVLSDLAWGPFAALWNANELAAAHDWLNE
                     RWSRLVRNRVLGADDPEAEFLQAIAFATLALFFTQNRNQEGARIVLDDALVALGKYRP
                     MFLGVRVDPIVAALSELRPLLAGLAAEDDCPLYPFAYPKFEYVR"
     gene            4062..4799
                     /locus_tag="VFV55_11095"
     CDS             4062..4799
                     /locus_tag="VFV55_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944890.1"
                     /translation="MNARDIPEDGTTPEVTAPECAFRCIAEAGPGTGMDGDGFVIDGP
                     DLDADHLRAAAAVVLPTQGAGRAAGLLASGARGVLLGEAALRDSSIVTMLAGEFGKER
                     IGVYVPARRLEVRWSFDTVSNADFRVLTPSLGAPAWEVLLADGAGTGTQALWWIDAML
                     ELGATQALLRVDIRDDADLNLCADCVERFGQRLWIGPLADEAPATADWIAFGHVRQMV
                     LPPALLAAQAQAQATRLAQTEPAMAAG"
     gene            4796..5260
                     /locus_tag="VFV55_11100"
     CDS             4796..5260
                     /locus_tag="VFV55_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944891.1"
                     /translation="MIALRQPVVAAVLALLAGTATAGSVTLLGGDKLVAALAEGPPCC
                     VVDARPAGTRALRPLPDAIAYRKGLKITPSAAVVVIADTDENAVRIGEEVARDSNAPK
                     VIAVMGGLATWKAATAPAQSGAAPPGFSFVIPKNTCESGTTLQHLRSGPRKP"
     gene            5260..6345
                     /locus_tag="VFV55_11105"
     CDS             5260..6345
                     /locus_tag="VFV55_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX4944892.1"
                     /translation="MRPELHQSRCTRYRFRYSECRRCADACPHDAIAFSDEGASIDAA
                     RCRNCALCTSACRTGAWESPEVPRVDLLKRAIKAPAWSFACAPSGAEADAVVPCLGAL
                     DAAMLAYLGKRGIPVELRGMGRCASCEHGERGAAMIEVQLEAVDLLGRASGEQWAQIT
                     IAEAEPAERPAPTAFAPGRRHLFRRILGGVATEVREAAKPVASAEAVPAKAIRAARAF
                     VTESRELLQIVARTKDATPLVVREHEALPLASLAIAGQCTACEACFRACPTGALQIRE
                     SDSAWSLAFALDKCVGCGVCLEVCQPRVLHTKPEFDIGSARPEAVLHSLGRQRCARCD
                     RLFTSPRPQHSCGVCTDDEEAFDAIFS"
     gene            6371..6778
                     /locus_tag="VFV55_11110"
     CDS             6371..6778
                     /locus_tag="VFV55_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944893.1"
                     /translation="MQWDFSPEDVVKGRAGYGLEEFRTDLAQEVRLNTPDASYEERAA
                     TFSLLYDLCHWLARGEDLEGFLATLAYDPPTCEFLRGVHPAILPNVEMLGAILQRMIM
                     DGVDAGMPLEEALVQAGAEHRRIVDGRMPAEVS"
     gene            complement(6814..7020)
                     /locus_tag="VFV55_11115"
     CDS             complement(6814..7020)
                     /locus_tag="VFV55_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944894.1"
                     /translation="MSDAESAPASSAGMTHRVWGEEPLWEVCDPDDDTVFEIGVRSLG
                     RVYAARSELFDAVCEIKERLRPRG"
     gene            complement(7067..7774)
                     /locus_tag="VFV55_11120"
     CDS             complement(7067..7774)
                     /locus_tag="VFV55_11120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX4944895.1"
                     /translation="MTIARDTPAIEEAQRLRYRVFGEGLGARLASGDRGIDEDEFDSH
                     CDHLIVRDVVSGAAIGTYRILAPESATAMGGYCSEREFDLSRLRPLRGRMAEVGRTCV
                     DPAYRSGVVMLLMWSTLAKYAIVNGYDHLIGCASVGLGDGGHGAASIFRRIAAEGLGP
                     EEYRVFPRCPLPLDRHEPGEPAPVPPLVKGYLSLGAWVCGEPAWDRDFNCADLPVLLA
                     IDRLDARFARHFLDQAA"
     gene            complement(7918..8694)
                     /gene="pstB"
                     /locus_tag="VFV55_11125"
     CDS             complement(7918..8694)
                     /gene="pstB"
                     /locus_tag="VFV55_11125"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX4944896.1"
                     /translation="MPTVLELENPKLRVRDLNFFYGQFHALKKVSLDIAEKKVTAFIG
                     PSGCGKSTLLRTFNRMFELYPEQRAVGEIVMDGENLLASKRDVSLIRAKIGMVFQKAT
                     PFPMSIYDNIAFGVRLFEKLPKARMDERVEWALRKAALWSEVKDKLHLSGSSLSGGQQ
                     QRLCIARGVAVKPEVLLLDEPCSALDPISTAKIEELIEELKNDYTVVIVTHNMQQAAR
                     CSDYTAYMYLGDLVEFGETDQLFLKPKEQATEDYITGRFG"
     gene            complement(8711..9556)
                     /gene="pstA"
                     /locus_tag="VFV55_11130"
     CDS             complement(8711..9556)
                     /gene="pstA"
                     /locus_tag="VFV55_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX4944897.1"
                     /translation="MDSTNPVYARRRRVNAIMMALSAAALAFGLFWLAWIIGTLLWEG
                     ASSLRPSLFTQPTPPPGGNGGLSNAILGSVMMAGIGTLIGTPVGILAGTYLAEFGRRG
                     WLAPATRFINDVLLAAPSIVIGLFVYAVYVARVGHFSGWAGALALSVIVIPVVVRTTD
                     DMLKLVPNSLREAAVALGCPTWKMITLVSYRAARAGILTGVLLAVARISGETAPLLFT
                     ALNNQFTSTNMNAPMANLPVVIFQFAMSPYDDWQRLAWGGAALITLLVLGINIAARML
                     FSKKH"
     gene            complement(9565..10503)
                     /gene="pstC"
                     /locus_tag="VFV55_11135"
     CDS             complement(9565..10503)
                     /gene="pstC"
                     /locus_tag="VFV55_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX4944898.1"
                     /translation="MTRNNALMDALFRHVTRFFAFVVFSLLAAILVTLLVGSTHSIEK
                     FGAGFLFNDNWDPVKEDFGALVPIYGTLVTSAIAMTIAIPVSFGIALFLTELSPPWLR
                     RPLGTAIEMLAAIPSIIYGMWGLFIFAPIFQEHVQPFLIGSLGTVPGLGVLFEGPPMG
                     IGMMTAGIILAVMVIPFITAVMRDVFDLVPPMLKESAYGLGSTTWEVTWRVVIPYTKV
                     GVIGGIMLGLGRALGETMAITFVIGNAHQLSPSLFAPGNSIASALANEFTEAVGDLYT
                     SALVELGLILFLITTIVLACSKLLLMQLAKQEGART"
BASE COUNT         1848 a         3557 c         3576 g         1595 t
ORIGIN      
        1 cgctgtactg acggcgccgt acgccgaaac gacaagagct aggagaacga acatgtctga
       61 taccaagttc acgcggcgga cttttctcaa ggtcagcgcc ggcaccacgg cggccgcggc
      121 cgccgccccg cgcctgctgc atgcgatgga gaccgagctg ggcggcaagg acgtgagtcc
      181 cgtcaccctg gccgaacgca aggccatccc gatcaactgc cacgtgtgca atatccagga
      241 cggcgccatt gcctacgtgg aaaacgggcg cgtcgtgaag ctcgagggca accccgagca
      301 cgtctcgacc cgcggccggc tctgcgccaa gggcaactcc ggcatgtggt acagctacga
      361 cccggatcgc atcctctatc ccctgaagcg cgtgggcgcc cggggcgaag ggaagtggaa
      421 gcgcatcacc tgggacgcgg cgctggccga agtcgcgcag aagctggacg cggcgctgaa
      481 ggaagacccg aacaccatca tgatgaagta cggccgcaac cgcaccggcg gcacgctcga
      541 ccgcttcatg cagacgctcg gctcggccac ggtggtgaac catacctcgg tgtgcgagtc
      601 gtcgaagaag atcggcatgg agcccacctg ggggccggac atcgagacgc cggacttcgc
      661 caacacgaag tacgtcctca acatcggctc caacatcctc gaggcggcct acttccacaa
      721 cccgctgtcg cagcgcatca ccgaggggcg cgtcgacaac cagatgaaga tcgtcacctt
      781 cgacgtgcgg ctgtcgaaca cggcgggctt ctccgacgag tggatcccgg tcttcccggg
      841 cacggacggc gcggtcgcgc tggcgctggg ccacgtgatc cttcgcgagg acctccagga
      901 ttccgagttc atcaacacct ggaccaacgt cacggtcgac gagctgaagg cgcattacaa
      961 ggattacacg cccgaatggg cgtcgaagat ctccggggtc ccggcggcga ccatcgagcg
     1021 catcgcgcgc gagttcgccc tggcgaagcc ggccacgctg ttcacctatc gcggcccggc
     1081 caagcacctc tacggctcgt acaacgagaa ggcgtgcatg atgctgccga tcatgaccgg
     1141 caatgtcgag acacgcggcg gctactgcct gccgcggggg atggggtggc cgcagcccga
     1201 tccgaagccg cccggcgcca agaacccctc ctacctgtcg aagccgccgg actatccgct
     1261 ggcgcaccac aaggtgagcc acctgacgcc gttcttcatc gccgacggca agcagaagat
     1321 caacgtctat ttcacctacc aggacaacgc ggtctacacc aacccgggcg cgcaggccgt
     1381 gtggggcaag ctgttccggg acgagaagct gctgccctac atcgtgtcga tgagctcgca
     1441 catgggcgag gagacggcgc tcgccgacat catcctgccc gactgcccgt acctcgagcg
     1501 ctgggagccg gaatcgatgc ccaactcgct gtggccatgg ctgggcatcc gccagcccgt
     1561 gcacgcctcg ctcggcgaat cgcgcgagaa cagggtcctg ctgcgcgaca tcatccacaa
     1621 gctcgatccg gacggcaaac gcggcatgaa gcagtactgg aacttcaagg acgccgagga
     1681 ctacatgcgc cagcacttcg acaacgtgcc ggggctcaag gaggcgggcg gcctcgactt
     1741 cctgaagaag cacggcgtct ggccgatcta cggcaagctc gacccgaaga ccggcaaggt
     1801 cacggacaag accggccgcg agatcaaggc ggagtacggc ctgcacaaga aggagctgag
     1861 cgccgccgaa atgaccggcg cggtggcgga caagtcgggc accatcacga aggacggcaa
     1921 ggccatcggg atcaagcgca acggcaggaa ctacgtcggc ttcccgaccg gcaaccgcct
     1981 catcaacgtc cgcgtcgagg aatgggagaa gtacggcttc aatccgatgc ccaccttcaa
     2041 gcgcatcccg tggcacgagg ccatgaagga agacgagatg atcctcacca ccttcaagct
     2101 caacgtgcac aagcagtcgc gcacggcggc ggtgaagtgg ctggccgaga tcgcccacag
     2161 caatcccgcc tggctcaacg cgcagacggc gaagaagctg ggcgtgaaga ccggcgacct
     2221 catccgcatc gagagcaagg tcggccacat ggtgaccaag gcctatgtga ccgaaggcat
     2281 ccagccgaag gtgatcgcca tcgcgaccgc gttcgggcac tgggagtacg gccggctggc
     2341 caccctgcgg ctcaaggaca agcccgcgtt cggcggccag gaagatgccg acatgagcaa
     2401 tgtgtggtgg gacgacaagg gcgtgcaccc gaacgccatc attcccgtgg tcgccgatcc
     2461 gatcggtggc tcgcaggggt ggatggacac cgtggtcaag gtgacgaagg cggggccgaa
     2521 cgacaagtac ggcgacaccc agggcaactg ggagaagcac tacgaggcgt acaaggaaac
     2581 gttgcgctac gcctacaccg gggacctgca ccgcaagatg caccccgaaa tggcctcgtg
     2641 ggctggcccg gacagcgtca aacccaaatc gggcggcgga cattgacgcc gctgctgcag
     2701 ccgggccatt ccccggacca ccggcgcacc ggcggtccgg gcctttcttc gggagtcgca
     2761 aatgctgaat gaactgctgc tggatgaccg gccggacccc gtgccggcac gcgcgcaggt
     2821 cgcgccctcg gcggaggacc ttcgccacga gtgcatcgcg cgcgccggcg tttaccggct
     2881 gatcgcgggc gtcttcgtcg aggagcccgg cgtcgaattc ctcgccacgc tgcgttcccg
     2941 gcccgcgctc gaggcgcttg ccgaggccgg tgtgcgcttc gagagcgatt tcctggatcc
     3001 ggatcccgcc acgctcgccg aagcgctggc cgtcgagtac acgacgatgt tcgccgcctc
     3061 gggcggcttc ccgccggtgg agtcgatccg cctcaccggc cgctaccagc aggcgccgca
     3121 cttcgacgtg aaggagacct accgccgcgc cggattcgtc ttgagcaagg ggcacttcca
     3181 ggtgctcgag gaccatctcg gcgtcgaact gctgttcgtg gcggaactgc tcgaccgcgc
     3241 cgtcgcggcg ctcgatcgcg gcgacctggc gggctaccgc gccgtcgatc gcgacatcaa
     3301 gcgtttctgg acgctgcacc tggcgcgctg ggtgcgcggc tattgccatc tcgtggagcg
     3361 cgcctcggag cattccctgt ttcgcgagat ggcgcgtttc ctcgccggct ttgccacgga
     3421 ggagatcgag tcgatgcgcc tgcgcgtcga cgacctcgac caggggcgcg aagtggtccc
     3481 caagagcgag gtccaggtgg ccttcaatcc cgacgagccc gtctgcggcg catgcggcaa
     3541 cggggacgaa ccgacactgc cgcgcggcgc gattccgatc gcgatccgtc cggccgcagc
     3601 ctgattcccg tgggagccca tgccatgctc gtcgagctga aatcccgggt gatcctgccg
     3661 cccgaccgcg gcgtcctctc cgacctggcg tgggggccct tcgccgcgct gtggaatgcc
     3721 aacgaactcg cggccgcgca cgactggctg aacgagcgct ggtcgcggct cgtgcgcaac
     3781 agggtgctcg gcgccgacga cccggaagcg gagttcctgc aggccatcgc cttcgcgacc
     3841 ctggcgctct tcttcacgca gaacaggaac caggaaggcg cccgcatcgt cctcgacgac
     3901 gcgctcgtcg cgctggggaa ataccgtccg atgtttctcg gcgtgcgcgt cgatccgatc
     3961 gtggccgcgc tctccgagtt gcgcccgctg ctcgccgggc tcgccgcaga ggacgattgc
     4021 cccctgtacc cgttcgccta tccgaagttc gaatacgtgc gatgaacgcc cgcgacattc
     4081 ctgaagacgg cacgacgccg gaagtgacgg cgccggagtg cgcgtttcgc tgcatcgccg
     4141 aggcgggccc gggtaccggc atggacggcg acggcttcgt gatcgacggc ccggacctcg
     4201 acgcggacca tcttcgcgcc gccgctgcgg tcgtgctccc cacgcagggt gcggggcgcg
     4261 cggccggcct gctcgcgtcg ggggcgcgcg gcgtcctcct cggagaggcg gcgctgcgcg
     4321 attcgtccat cgtgacgatg ctcgccggcg agttcgggaa ggagcggatc ggcgtttacg
     4381 ttcccgcccg gcgcctcgag gtgagatggt ccttcgacac ggtctccaat gcggacttca
     4441 gggtgctgac gccgtccctc ggggcgcccg cctgggaagt gctcctggcc gacggcgcgg
     4501 gaacgggaac gcaggcgctc tggtggatcg acgcgatgct cgagctcggc gccacgcaag
     4561 cgctcctgcg cgtggacatc cgcgacgacg cggacctgaa cctgtgcgcc gactgcgtgg
     4621 agcgcttcgg gcagcgcctc tggatcgggc ctctcgccga cgaggcgcct gcgactgccg
     4681 actggatcgc gttcggtcat gtgcggcaga tggtgctgcc cccggccctg ctggcggcgc
     4741 aggcgcaggc gcaggcgacc cgtctcgccc agacggagcc cgccatggcg gcgggatgat
     4801 cgcgctccgg cagcccgtcg tggccgccgt ccttgccctg ctcgccggta cggcaacggc
     4861 cggcagcgtc acgctgctcg gcggagacaa actcgtcgcc gcgctggccg aggggccgcc
     4921 gtgctgcgtg gtggatgccc gtcccgcggg gacgcgcgcg ctgcgcccgc tgcccgatgc
     4981 gatcgcctat cgcaagggcc tgaaaatcac gccttccgcc gccgtcgtgg tcattgccga
     5041 cactgacgag aatgccgtgc gcatcggcga ggaggtcgcc cgcgacagca acgcacccaa
     5101 ggtgatcgcg gtcatggggg gactcgccac ctggaaggcg gccacggcac cggcgcaatc
     5161 cggagcggca cccccggggt tcagcttcgt gatcccgaag aacacctgcg aatcgggcac
     5221 gacgctgcag catttgcgct cgggtccccg gaaaccgtga tgcgcccgga actgcaccag
     5281 tcccgctgca cccgctaccg tttccggtac agcgaatgcc gccggtgcgc cgacgcgtgt
     5341 ccccacgatg ccatcgcctt ctccgacgag ggcgcgtcca tcgacgctgc ccgatgccgc
     5401 aactgcgccc tgtgcacgag cgcgtgccgg accggggcct gggaatcgcc ggaagtaccg
     5461 cgcgtggacc tgctcaagcg ggccatcaag gcgcccgcct ggagcttcgc gtgcgcgccc
     5521 tcgggtgcgg aggcggacgc cgtggtgccg tgcctcggtg cgctcgatgc cgcgatgctc
     5581 gcctacctcg gcaagcgcgg aatcccggtc gagctgcgcg gcatggggcg ctgcgcctcg
     5641 tgcgagcacg gggagcgcgg cgcggccatg atcgaagtcc agctcgaagc cgtggacctt
     5701 cttggccggg catccggcga gcaatgggcg cagatcacca tcgcggaagc agaacccgcg
     5761 gagcgtcccg cgccgacggc attcgcgccc ggccggcgcc acctgttccg gcgcatcctc
     5821 ggcggcgtgg cgaccgaggt ccgcgaggct gcgaagcccg ttgccagcgc ggaggcggta
     5881 cccgccaagg ccatccgcgc cgcgcgcgcc ttcgtgaccg agtcgcgcga gctgctgcag
     5941 atcgtcgcgc gaacgaagga tgcgaccccg ctggtggtgc gcgagcacga ggcgctgccg
     6001 ctcgcgagcc tcgccatcgc ggggcaatgc accgcttgcg aagcgtgctt ccgcgcctgc
     6061 ccgaccggcg cactgcagat ccgggagagc gactcggcct ggtcgctcgc cttcgcgctc
     6121 gacaagtgcg tgggctgcgg ggtctgcctc gaggtgtgcc aaccccgcgt gctgcatacg
     6181 aagcccgagt tcgacatcgg ttccgccagg ccggaagcgg tgctgcactc cctgggccgg
     6241 cagcgttgcg cgcgctgcga ccgtctcttc acgtcgccgc ggccccagca ttcgtgcggg
     6301 gtctgcaccg acgacgagga ggcattcgac gccatcttca gctgaccgct tcgacacgaa
     6361 aggcgctgcc atgcaatggg atttctctcc ggaagacgtc gtgaaaggcc gggccggcta
     6421 cggcctcgag gagtttcgca ccgacctcgc gcaggaagtc aggttgaaca cgcccgacgc
     6481 gagctacgag gagcgcgcgg cgacgttcag ccttctctac gacctgtgcc actggctcgc
     6541 gcgcggcgag gatctcgagg ggttcctcgc gacgctcgcc tacgatccgc cgacgtgcga
     6601 gttcctgcgc ggcgtgcatc cggcgatcct gcccaatgtc gagatgctcg gcgcgatcct
     6661 gcaacggatg atcatggatg gcgtcgatgc cgggatgccc ctcgaggaag cactcgtgca
     6721 agccggcgcc gaacaccggc ggatcgtcga cggcaggatg cctgccgagg tgagctgaag
     6781 cccgcggcaa ccggagaaga gaaggagcgg ccttcatccc cgaggccgca ggcgttcctt
     6841 gatttcgcag acggcgtcga acaactcgct tctcgcggca tagacgcgcc ccagtgaccg
     6901 cacgccgatc tcgaaaaccg tgtcgtcatc ggggtcgcag acttcccaca ggggctcttc
     6961 tccccacacg cgatgcgtca tcccggcgct gctcgcaggg gcactctctg catcgctcat
     7021 gagttcaatc gtcggtaccg tcatggcagg gagcaaggat gggcgatcag gcggcctgat
     7081 cgaggaagtg ccgggcgaac ctcgcgtcga gacggtcgat ggcgagaagc accggcaggt
     7141 cggcgcaatt gaagtcgcgg tcccaggcag gctccccgca gacccacgct ccgaggctaa
     7201 ggtagccctt cacgagcggc ggcaccggcg ccggctcgcc gggctcgtgg cgatcgagcg
     7261 gcagggggca ccggggaaag acccggtact cctccggtcc caggccctcc gccgcgatcc
     7321 gccgaaagat ggaggctgcg ccatggccgc cgtcccccag gcccacgctg gcgcacccga
     7381 tgagatggtc gtaaccgttg acgatggcgt acttcgccag ggtggaccac atgagcagca
     7441 tcacgacacc cgatcgatag gccggatcga cgcaggttcg gcccacctcg gccatgcggc
     7501 cgcgcagcgg acggagccgc gagaggtcga actccctctc cgagcagtag ccacccatcg
     7561 ctgtcgccga ctccggcgcg agaatgcgat aggtgccgat cgccgcgccc gacaccacgt
     7621 cgcgcacgat caggtggtcg cagtggctgt cgaattcatc ctcgtcgatg ccacgatcac
     7681 cggatgccag tcgcgcgcca agcccctccc cgaagacgcg gtaacgcaat cgctgcgctt
     7741 cctcgatggc cggggtatcc cgggcgatag tcactgcgaa acgggaggtg ttcccgggaa
     7801 accggggtcc tgcggtcatc gtgttctcgc atgcgagttg gaatgcgcat acggtaaagg
     7861 cgccggatga ccgcgccgtg aatgtttcgc tacggtttgc ttacggcagg atggacgcta
     7921 gccgaaacgc ccggtgatgt agtcctcggt agcctgctct ttcggcttga ggaagagttg
     7981 gtcggtctcg ccgaattcca cgaggtcccc gaggtacatg taggcggtgt agtccgagca
     8041 gcgcgccgcc tgctgcatgt tgtgcgtgac gatcacgacc gtgtagtcgt tcttgagctc
     8101 ctcgatgagc tcctcgatct tggccgtcga gatcgggtcg agcgccgagc agggctcgtc
     8161 gagcagcagc acctccggct tcaccgcgac gccgcgcgcg atgcacaggc gctgctgctg
     8221 gccgcccgag aggctcgagc cgctcaggtg cagcttgtcc ttcacctcgc tccacagcgc
     8281 cgccttgcgc aacgcccact cgacgcgctc gtccatccgc gccttcggga gcttctcgaa
     8341 gaggcgcacg ccgaaggcga tgttgtcgta gatcgacatc gggaagggcg tggccttctg
     8401 gaacaccatg ccgatcttgg cccggatgag cgacacgtcg cgcttcgagg caaggaggtt
     8461 ctcgccgtcc atcacgatct cgcccacggc gcgctgctcg gggtagagct cgaacatgcg
     8521 gttgaaggtc ctgagcagcg tcgacttgcc gcagcccgag gggccgatga aggcggtcac
     8581 cttcttctcg gcgatgtcga gcgagacctt cttcagggcg tggaactggc cgtagaagaa
     8641 attcaggtcg cgcacgcgca gcttggggtt ctccagttca agcacggtag gcattgcgtt
     8701 cggttctccg tcagtgcttc ttcgaaaaca gcatgcgggc ggcgatgttg atgcccagca
     8761 cgagcagcgt gatgagcgcc gccccgcccc aggcgaggcg ctgccagtcg tcgtacgggc
     8821 tcatcgcgaa ctggaagatc accaccggca ggttcgccat cggcgcgttc atgttggtgg
     8881 aggtgaactg gttgttgagc gcggtgaaca gcagcggcgc cgtctcgccc gagatgcgcg
     8941 ccacggcaag cagcaccccg gtgaggatgc cggcgcgcgc cgcgcggtag ctcaccaggg
     9001 tgatcatctt ccacgtgggg cagcccagcg ccactgcggc ctcgcgcagg ctgttgggga
     9061 cgagcttcag catgtcgtcg gtggtgcgca cgaccaccgg gatcacgatc accgaaaggg
     9121 ccagcgcgcc cgcccatccc gaaaagtggc ccaccctggc gacatacacc gcgtagacga
     9181 agaggccgat cacgatggaa ggcgcggcga gcagcacatc gttgatgaag cgcgtggccg
     9241 gggcgagcca gccgcgccgg ccgaactcgg caaggtaggt tcccgccagg attccgaccg
     9301 gtgtaccgat aagggtgcca atcccggcca tcatgacgct gccgagaatc gcgttcgaga
     9361 gcccgccatt gccgccgggc ggtggcgtcg gctgcgtgaa aagcgaaggc cgcaaggacg
     9421 aagcgccttc ccagaggagc gtgccgatga tccacgcgag ccagaagagc ccgaaggcca
     9481 gcgcggcggc cgagagcgcc atcatgatgg cgttgacgcg gcggcgccgg gcgtagacgg
     9541 ggttcgtcga atccatcgcc gtgctcacgt ccgcgctccc tcctgcttcg cgagctgcat
     9601 caacagcagc ttcgagcacg ccagcacgat ggtggtgatg aggaagagga tcaggcccag
     9661 ctcgacgagc gccgaggtgt agaggtcgcc cacggcctcg gtgaactcgt tggccagggc
     9721 ggaggcgatg ctgttgccgg gcgcgaacag cgacgggctc aactggtggg cattgccgat
     9781 gacgaaggtg atcgccatgg tctcgcccag cgcgcgcccc aggcccagca tgatgccgcc
     9841 gatgacgccg actttcgtat agggaatcac cacgcgccac gtcacctccc acgtggtgga
     9901 cccgagcccg taggccgact ccttgagcat cggcggcacg agatcgaaga cgtcgcgcat
     9961 caccgcggta ataaatggga tcaccatgac ggcaaggatg atgccggcgg tcatcatccc
    10021 gatgcccatg ggcggaccct cgaagagaac gcccaggccg ggaaccgtgc cgagcgaccc
    10081 gatcaggaaa ggctgcacgt gttcctggaa aatcggcgcg aagatgaaca gtccccacat
    10141 gccgtagatg atgctgggga tcgccgcgag catctcgatg gccgttccca gcggccggcg
    10201 cagccagggc ggcgagagct cggtgaggaa gagggcgatg ccgaagctca cgggaatcgc
    10261 gatcgtcatc gcgattgccg aggtgaccag cgtgccgtag atgggaacga gcgcgccgaa
    10321 atcctccttc accgggtccc agttgtcgtt gaagaggaag ccggcgccga acttctcgat
    10381 ggagtgcgtg ctgccgacga ggagcgtgac gaggatcgcc gcaagcaggc tgaacaccac
    10441 gaacgcgaag aaccgcgtga catggcggaa gagcgcgtcc atgagggcgt tgttccgggt
    10501 catcgaggca ttttcccgga tttccggcgg ccgagggccg cctgttcagt agaccggctt
    10561 gccggccggg tccttg
//
LOCUS       DASUEV010000352         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_452733, whole genome shotgun sequence.
ACCESSION   DASUEV010000352 DASUEV010000000
VERSION     DASUEV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452733"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFV55_11140"
     CDS             <1..760
                     /locus_tag="VFV55_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX4944899.1"
                     /translation="DAIIQQPHGIFLVTGPTGSGKTTTLYAALTRLDRKTRNILTVED
                     PVEYELDGIGQTPVVPKIDMTFARALRAILRQDPDVVMIGEIRDLETAQIAVQASLTG
                     HLVLATLHTNDAPGAVTRLADMGIEPYLLASTLSGVLAQRLVRKLCPACKTPYEPDAV
                     ERGAFPHDTPERLYRAVGCAACRFSGYKGRTGIYELLTIDEDLRRLVHDTSAEREIRE
                     RATARGMTRLREDGLRWVRNGATSLDEVLRVTRA"
     gene            790..2004
                     /gene="gspF"
                     /locus_tag="VFV55_11145"
     CDS             790..2004
                     /gene="gspF"
                     /locus_tag="VFV55_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX4944900.1"
                     /translation="MPGFRYQAYSIEGRLHKGVLEADSARQARSVLRDQGLTPFRVDV
                     IAANDPAGGRHFRALSLSSAELTRLTRSLASLIEAGLTVEQAFNALIEQAEVERERQV
                     LAALRGEVIAGSTIAKALEGFPRVFPELYRTLVAAGESSGQLPRVLAKLADYLEERHA
                     LRGRLALALIYPAIVMGVSLLVMGALLVYVVPQVVQVYSHAKQALPILTRMLIALSGF
                     LQVTWPAWIALAVAAGVGLRVALSRPASRARIHRFLFGAPVVGKALRHLDSARLAATL
                     AILVGSRVPILAALEAGEGVMTLVPMREALASAARGVREGMPLSRALSSTGAFPPVMV
                     HLIAGGEATGRLDESLERAARQQQADLSTRLATVVAVFEPALIVIMGVLVLAIVLAIL
                     LPIFNLNQLVGR"
     gene            2010..>2153
                     /locus_tag="VFV55_11150"
     CDS             2010..>2153
                     /locus_tag="VFV55_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX4944901.1"
                     /translation="MKRQSAFTLIEILVVVAILGILAAIVVPRVMDRPDEAKRVAAKA
                     DVAA"
BASE COUNT          288 a          828 c          710 g          327 t
ORIGIN      
        1 ggacgcgatc atccagcagc cgcacggcat cttcctcgtg accggcccca cgggctcggg
       61 caagaccacg acgctgtacg ccgcgctcac gcgcctggac cgcaagacgc gcaacatcct
      121 caccgtcgag gacccggtcg agtacgagct ggacggcatc ggccagacgc cggtcgtccc
      181 gaagatcgac atgaccttcg cgcgggcgct gcgtgccatc cttcgccagg accccgacgt
      241 ggtgatgatc ggcgagatcc gcgacctcga gaccgcgcag atcgcggtgc aggcctcgct
      301 caccggccac ctcgtgctcg cgaccctgca caccaacgac gcgcccggcg ccgtgacgcg
      361 cctcgcggac atggggatcg agccctacct gctcgcctcc acgctctccg gcgtgctggc
      421 ccagcgcctg gtgaggaagc tctgccccgc ctgcaagaca ccctacgaac ccgatgcagt
      481 cgagcgcggc gccttcccgc acgacacgcc ggagcgcctc taccgggccg tcggctgcgc
      541 cgcctgccgg ttctccggct acaagggccg caccggcatc tacgagctcc tcaccatcga
      601 cgaggacctg cgccgcctgg tgcacgacac gtccgccgag cgcgagatcc gcgagcgggc
      661 gacggcccgc ggcatgacgc ggcttcgcga ggacgggctg cgctgggtgc gcaatggcgc
      721 cacctcgctc gacgaggtgc tgcgcgtgac gcgcgcctag cccgctccct gggaggggga
      781 aaagagcgaa tgccgggctt ccgctaccag gcgtactcga tcgagggcag gctgcacaag
      841 ggcgtgctcg aggccgacag cgcgcgccag gcgcgctcgg tcctgcgcga ccaggggctc
      901 acgccgttcc gggtcgacgt catcgccgcc aacgatccgg cgggaggccg ccacttccgc
      961 gccctgtcgc tttcgtcggc ggagctcacg cgcctcacgc gcagcctcgc ctcgctcatc
     1021 gaggcgggac tcaccgtcga gcaggccttc aacgcgctca tcgagcaggc cgaggtcgag
     1081 cgcgagcggc aggtgctcgc ggcattgcgc ggcgaggtga tcgcgggttc cacgatcgcc
     1141 aaggcgctgg agggctttcc ccgcgtcttc cccgagctct accgcacgct cgtcgcggcg
     1201 ggcgagtcct cggggcaact gccgcgcgtg ctggcgaagc tcgccgatta cctcgaggag
     1261 cgccacgccc tgcgcggccg gctggcgctc gccctcatct acccggccat cgtgatgggc
     1321 gtgtcgctcc tggtgatggg cgcgctcctc gtctacgtcg tcccgcaggt ggtgcaggtg
     1381 tacagccacg cgaagcaggc gctgccgatc ctcacgcgca tgctgatcgc cttgtcgggt
     1441 ttcctgcagg tcacctggcc tgcctggatc gcgcttgccg ttgctgccgg cgtcggcctc
     1501 cgggtcgccc tctcccgccc tgcgtcgcgc gcacgcatcc accgcttcct cttcggcgca
     1561 ccggtcgtgg gcaaggcgct tcgccacctc gacagcgcgc ggctggcggc cacgctcgcg
     1621 attctcgtgg gaagtcgcgt cccgatcctg gccgcgctcg aggccggcga gggcgtgatg
     1681 accctcgtcc cgatgcgcga ggcactggcc tccgcggcgc gcggagtgcg cgagggcatg
     1741 ccgctttccc gcgcgctctc ttccaccggc gccttcccgc cggtgatggt ccacctcatc
     1801 gccgggggcg aggcaacggg caggctcgac gaatcgctcg agcgcgcggc gcgccagcag
     1861 caggcggatc tctcgacgcg gctcgccacc gtcgtcgcgg ttttcgagcc cgcgctcatc
     1921 gtcatcatgg gcgtgctcgt gctcgccatc gtgctcgcca tcctgctgcc catcttcaac
     1981 ctcaaccagc tcgtcggccg gtagacccca tgaaacgcca atccgccttc accctcatcg
     2041 agatcctcgt cgtcgtggcg atcctcggca tcctcgcggc catcgtcgtg ccgcgcgtca
     2101 tggaccggcc cgacgaggcc aagcgcgtgg ccgccaaggc cgacgtcgcc gcc
//
LOCUS       DASUEV010000353         9133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_197409, whole genome shotgun sequence.
ACCESSION   DASUEV010000353 DASUEV010000000
VERSION     DASUEV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197409"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFV55_11155"
     CDS             <1..663
                     /locus_tag="VFV55_11155"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEX4944902.1"
                     /translation="ADRNVKAKITRDGIFVEELDRNPGRLIPEQYRGKQANGVRIDLN
                     QPMKAILAELSKHPVSTPLLLNGAIVVGRDIAHAKFKELIDAGKPVPEYLLKHPIYYA
                     GPAKTPKGKASGSFGPTTAGRMDSYVDLLQGKGASMIMIAKGNRSQQVTDACRKYGGF
                     YLGSIGGPAAVLAEENIRKVECIDFPELGMEAVWKIEVEDFPAFILVDDKGNDFFKQL
                     GL"
     gene            complement(715..1587)
                     /locus_tag="VFV55_11160"
     CDS             complement(715..1587)
                     /locus_tag="VFV55_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944903.1"
                     /translation="MPTTSLRPALGRDALDYRDALHPGQPLVVHTYRPAAHGPDDPVV
                     LVQHGVKRNGDEYRDFWIEAAEKHRLLIVATTFGDEAFPKPENYNNGSVVAPDGSVRP
                     REDWLYAILPRVVEALRAGGVTRRARVRLFGHSAGGQFVHRLLATQDHSIYEAAMPAN
                     PGWYTLPTLDRPFPEGLGGLGLGRNDLARWFAYPMTILAGDRDIDTADEHLPRNPEAL
                     AQGPTRFARAHFFHDMAHREAARLGVACRWTLVTIPGVGHDGCAMGKAAAAIWFEGRV
                     PGAGELAAATSPVA"
     gene            complement(1593..2591)
                     /locus_tag="VFV55_11165"
     CDS             complement(1593..2591)
                     /locus_tag="VFV55_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944904.1"
                     /translation="MTPPVLEVRGASKHFARRPTLAERILMAAGRAEPPPVVRAVEGV
                     DLAIHRGEVLGLVGESGCGKSTLARLVTGIHPPTAGEVFFEGRPVGQLHGRERLDYLL
                     AVQMIFQDPYASLDPRMRVHRIVGEALAVHRLVPPGELDAAIDQALADVGLDTAYRDR
                     YPHQFSGGQRSRIGIARALAVKPRVLVCDEPVAALDVSIQAQVINLFMDLRERHALTY
                     LFVSHNLGLVRLICDRVAIMYLGRIVETGPVAELFEVPAHPYTQALLHAIPRIHGRKR
                     AFEVVKGELPSPLAPPPGCPFHPRCPLATPECRETRPELREIAPGRHAACHLLNRH"
     gene            complement(2588..3568)
                     /locus_tag="VFV55_11170"
     CDS             complement(2588..3568)
                     /locus_tag="VFV55_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4944905.1"
                     /translation="MSAPLLQVRGLRTVFHLADGAWAAVDGVDLEVDRGQVLGLVGES
                     GSGKTVTGFSIVGLVDPPGEVIAGEVIFAGEDLRHAPEARLRELRGDRIAMIFQDPMM
                     TLNPVLTIGEQMAEAVLEHGGTDEAAALSLAGEALTRVGIASAGSRLKQYPHEFSGGM
                     RQRVAIATALLNKPDLIIADEPTTALDVTIQGQILFEMQKLARETGAALVWITHDLAV
                     VAELADRIAVMYAGRIVEAGTLAEVLDNPRHPYTRGLLDSVPDVGVRGQRLAQIDGMA
                     PRLDTRPAGCAFAPRCPRASARCREVDPPETAEAGRRYRCHHPLAQGEAR"
     gene            complement(3565..4464)
                     /locus_tag="VFV55_11175"
     CDS             complement(3565..4464)
                     /locus_tag="VFV55_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944906.1"
                     /translation="MSAAPVLPGDDTTLRRQVLRSLRRQPTARGAAIVLALIVAFALI
                     GPFIAPQNPYDLTALNMMDNRLAPGGKGMAGFTFWLGTDAQGRDMLSAILYGLRTSLF
                     VGLAATAGALAVGVSVGLAAAQFGGRVDAFLMRVVDFILGFPSILVALVLLASIGRGV
                     DKVILAIVVVQWANYARLMRAAALVERRKEYIEAAMNQGLPTAYIMRAHLLPNSIGAV
                     LVVATVSIAGAITLEATLSFLGVGVPVTEPSLGLLIANGFEFLLSGEYWISLFPGLAL
                     LALIMSINVVGERLRLSFNAHRH"
     gene            complement(4461..5438)
                     /locus_tag="VFV55_11180"
     CDS             complement(4461..5438)
                     /locus_tag="VFV55_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX4944907.1"
                     /translation="MATYALQRLIQSFFVLLAASIVVFLAVYGIGDPVELLVPPQASA
                     LERDELVRRLGLDLPLWRQYLVFAWNALHGDLGRSFVHGVPAIEIILSRLPATFELVL
                     VAMTLALAFGIPLGLVAGLNPEARLSRVIMGGTVLGYSLPNFWKGMMLILVFSVLLGW
                     LPTSGRGATVQIIGVGTSLLTLDGLRHLILPALNLAIPNMALMIRLVAAGTTEAKAQD
                     YVKYARAKGVRPRRIVGRHILRNILIPVVTVVGIEFGSLIAFSTITETVFAWPGMGKL
                     LIDSIYQLDRPVVVSYIMLATFLFVSVNLLVDLLYAALDPRVRLVGEPS"
     gene            complement(5459..7072)
                     /locus_tag="VFV55_11185"
     CDS             complement(5459..7072)
                     /locus_tag="VFV55_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944908.1"
                     /translation="MKCFRLLPLAAAAALALAAASAGAQTLRIALSSEPTSVDPHYHV
                     LAPNNALAAHIYDALTDLDEQQRVVPGLATGWKNDGANKWTFQLRKGVKFSNGKPFTA
                     DDAIFTYCRVEKNETSIAGGNKLIIRNMRSVEAPDPYTLVVTTAEPEPLLPRLLAEIG
                     ILSASIAAHGKIAFNLAANCGVTGPWPSVTQFNEGSVAIGTGPFRLKSYVRGSAIELV
                     RNDDYWGEKSPWQAVKMVPVTNAGPRLAGLIAGDYDVVENPAARDLGRIKEDKRLAYV
                     VTPSSRVIFFQLDVARSPSPLVKDDKGGNPLQDARVRKAMSIAIDRDAIAKRLMDGGA
                     HPAYQFLPTGMYGTLANPSVIKFDPAAAKKLLAEAGYANGFDITLSTPNDRYINDGQI
                     AQAVAGYLSQVGIRTKVDAMTRAIYFPRRAKKEFSFSMGGWGAAEASSFLRYWAAAPD
                     EKKTRGTSNYGGLDDAALNELVGKALVTMDDDKRADILRLAVARLLETGAFIPLHFES
                     TIWAHRADLVVKGRTDQYTLAMSVAPAKK"
     gene            complement(7705..8718)
                     /locus_tag="VFV55_11190"
     CDS             complement(7705..8718)
                     /locus_tag="VFV55_11190"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX4944909.1"
                     /translation="MRFCGIDLHSNNCVVVVTDEADRKLLSRRCRNDLATILSMLEPH
                     REELAGVVVESTYNWYWLVDGLRAAGYEVKLANTVAMKRYDGLKHSGDEDDAAFLAHL
                     LRLGILPTGYIHPPAQRALRDLARKRAQLVRSRTQHVLSVENILARERGARLASKAIQ
                     RLNATTVEQLGLGADVSLAVKSSVAVIATLNQEIALLETRLLKAVKPRPEYALLKSIT
                     GIGEVLSIVIMLETGEIGRFAGVGNFASYTRCVDSAHYSNGKKKGEGNTKNGNVYLAW
                     AFVEAANFARRFSAEAKCFFERKKARTNTVIATKALAHKLARAAYHVLKDRQPFDVKR
                     CFT"
BASE COUNT         1468 a         3069 c         3186 g         1410 t
ORIGIN      
        1 gccgaccgca acgtcaaggc gaagatcacg cgggacggca tcttcgtcga ggaactggac
       61 cgcaatccgg gccgcctcat cccggaacaa taccggggca agcaggcgaa cggtgtccgc
      121 atcgacctca atcagccgat gaaggcgatc ctggccgagt tgtccaagca cccggtatcg
      181 accccgctcc tgctcaatgg cgccatcgtc gtgggacgcg acatcgcgca cgcgaaattc
      241 aaggaactca tcgacgccgg aaagcccgtg ccggaatatc ttctcaagca cccgatctac
      301 tacgcgggtc cggccaagac cccgaaagga aaggcgtccg gctccttcgg cccgacgacc
      361 gccggccgga tggattccta tgtggatctg ctgcagggca agggcgccag catgatcatg
      421 atcgccaagg gcaaccgctc gcagcaggtg accgatgcgt gcaggaagta cggcgggttc
      481 tacctcggct ccatcggcgg cccggccgcg gtgctggcag aggagaacat caggaaagtc
      541 gaatgcatcg acttcccgga actggggatg gaagcggtgt ggaagatcga ggtggaggac
      601 ttcccggcgt tcatcctggt ggacgacaag gggaacgact tcttcaagca gttggggctg
      661 tagggcactg aagtcccggc gataccctcc gctacgcgga accggtaccg gcactcacgc
      721 gacgggcgac gtggctgccg cgagttcgcc cgcgcccggc acgcgcccct cgaaccagat
      781 ggccgccgcg gccttgccca tggcgcatcc gtcgtggccc acgcccggga tcgtgacgag
      841 cgtccagcgg caggcgacgc cgagccgggc cgcctcgcgg tgcgccatgt cgtggaagaa
      901 atgcgcccgg gcaaagcgcg tcggtccctg cgcgagcgct tcgggattgc gcggcaggtg
      961 ttcgtccgcc gtgtcgatgt cgcggtcgcc cgcgagtatc gtcatcgggt aggcgaacca
     1021 gcgcgcgagg tcgttgcgtc cgaggcccag cccgcccagc ccctcgggga acgggcgatc
     1081 cagggtcggc agggtgtacc acccgggatt ggcgggcatg gcggcttcat agatcgaatg
     1141 atcctgcgtg gccaacagcc gatgcacgaa ctgtccgccg gccgaatgcc cgaagaggcg
     1201 cacccgggcc cggcgcgtga cgccccccgc gcgcaacgcc tccacgacgc gaggcaggat
     1261 cgcgtagagc caatcctcgc gcgggcgcac gctcccgtcg ggtgccacga cgctgccgtt
     1321 gttgtagttc tccggcttgg ggaacgcctc gtcgccgaag gtcgtcgcca cgatgagcag
     1381 gcgatgcttc tcggccgcct cgatccagaa gtcgcggtat tcgtcgccgt tgcgcttgac
     1441 gccgtgctgc acgagcacca cgggatcatc gggaccgtgc gcggcgggac ggtaggtgtg
     1501 gacgacgagg ggctggccgg ggtgcagtgc gtcgcggtag tcgagggcgt cgcggccgag
     1561 ggcgggtctg agggaggtcg ttggcatgga gatcagtgtc ggttgagaag gtggcaggcg
     1621 gcgtggcggc cgggcgcgat ctcgcgcagc tccggcctcg tctcgcggca ttcgggcgtc
     1681 gccaatgggc aacgcggatg gaagggacat cccggcggcg gcgcgagcgg cgacggcagc
     1741 tcgcccttca ccacctcgaa cgcgcgcttg cggccgtgga tgcgcggtat cgcgtgaagg
     1801 agcgcctgcg tgtacgggtg cgcgggcacc tcgaagagtt cggccaccgg ccccgtctcg
     1861 acgatgcgcc ccaggtacat gatcgcgacg cggtcgcaga tgaggcgcac cagcccgagg
     1921 ttgtggctga cgaagaggta ggtgagcgcg tggcgctcgc gcaggtccat gaacaggttg
     1981 atcacctgcg cctggatgga aacgtcgagc gcagccaccg gctcgtcgca caccagcacg
     2041 cgcggcttca ccgcgagcgc gcgggcaatg ccgatgcggc tcctctggcc gccggagaac
     2101 tggtgcggat agcggtcgcg ataggccgtg tcgagcccga cgtcggcgag cgcctggtcg
     2161 atcgccgcgt ccagctcccc ggggggcacg aggcggtgca cggcgagcgc ctcgcccacg
     2221 atgcggtgca cgcgcatgcg cgggtcgagc gacgcgtacg ggtcctggaa aatcatctgc
     2281 accgcgagca ggtagtcgag gcgctcgcgg ccgtgcaact gccccaccgg ccgcccctcg
     2341 aagaacacct cgcccgccgt gggcggatgg atgcccgtga ccaggcgcgc gagggtggac
     2401 ttgccgcagc ccgattcgcc cacgagcccc agcacctcgc cgcggtgaat ggccaggtcc
     2461 acgccttcga ccgcgcgcac caccggcggg ggctcggccc ggccggccgc catcaggatg
     2521 cgctcggcga gcgtcgggcg gcgcgcgaag tgcttgctgg ccccgcggac ctcgaggacg
     2581 ggcggcgtca tcgcgcctct ccctgggcca gcgggtgatg gcaacggtag cgccgccccg
     2641 cctccgccgt ctccggcgga tcgacctcgc ggcatcgcgc cgaggctctc gggcagcgcg
     2701 gcgcgaacgc gcaccccgcc ggccgcgtgt cgaggcgcgg ggccatgccg tcgatctggg
     2761 cgagccgctg cccgcgcacg ccgacgtcgg gcaccgagtc gaggagcccg cgcgtgtacg
     2821 gatggcgcgg gttgtcgagc acctcggcca gcgtgcccgc ctcgacgatg cgcccggcat
     2881 acatcaccgc gatgcggtcc gcgagctcgg ccaccaccgc gaggtcgtgc gtgatccaga
     2941 cgagcgccgc gcccgtctcg cgcgcgagct tctgcatctc gaagaggatc tggccctgga
     3001 tcgtcacgtc gagcgcggtg gtgggctcgt ccgcgatgat gaggtcgggt ttgttgagga
     3061 gcgcggtcgc gatcgccacg cgctggcgca tccccccgga aaactcgtgc gggtattgct
     3121 tcaggcgcga cccggccgag gcgatcccga cgcgggtaag cgcctcgccc gcgagagaca
     3181 gcgccgccgc ctcgtccgtc ccgccgtgct cgagcaccgc ctcggccatc tgctcgccga
     3241 tggtgagcac cgggttcagt gtcatcatcg ggtcctggaa gatcatcgcg atgcggtcgc
     3301 cgcgcagctc gcgcagccgc gcctccggag catggcgcag gtcctcgccc gcgaagatca
     3361 cttctccggc aatcacctcg ccgggcggat cgacgagccc cacgatcgag aagccggtca
     3421 ccgtcttgcc cgagcccgat tcgcccacga gccccagcac ctggccgcga tccacctcga
     3481 ggtccacgcc gtccacggcg gcccaggcgc cgtcggcgag gtggaagacg gtccgcaggc
     3541 cgcggacctg aagcagcggc gcgctcaatg ccggtgggcg ttgaagctga gccgcagccg
     3601 ttcgccgacg acgttgatgg acatgatgag ggcgaggagc gccaggccgg gaaagagcga
     3661 gatccagtac tcgcccgaga gcaggaactc gaagccgttc gcgatcagca ggcccagcga
     3721 cggctccgtc accggaacgc cgacgccgag gaacgacagc gtggcctcga gcgtgatggc
     3781 ccccgcgatg ctgaccgtgg ccaccacgag caccgcgccg atgctgttgg ggagcaggtg
     3841 cgcccgcatg atgtaggccg tgggcagccc ctggttcatc gcggcctcga tgtactcctt
     3901 gcgccgctcg acgagcgccg ccgcgcgcat gaggcgggca tagttcgccc attgcacgac
     3961 gacgatcgcg aggatcacct tgtccacgcc ccgcccgatg gaggcgagca gcacgagcgc
     4021 caccaggatc gagggaaagc cgaggatgaa atccaccacc cgcatcagga aggcgtccac
     4081 gcgcccgccg aactgcgccg cggcgagccc cacgctcacg cccaccgcga gcgcgcccgc
     4141 cgtggccgcg aggcccacga agaggctggt gcgaaggccg taaaggatcg cgctcagcat
     4201 gtcgcgcccc tgcgcatcgg tgccgagcca gaaggtgaaa cccgccatgc ccttcccgcc
     4261 gggcgccagg cggttgtcca tcatgttgag cgccgtgagg tcgtacgggt tctgcggcgc
     4321 gatgaagggc ccgatgagcg cgaacgcgac gatgagtgcc agcacgatcg ccgcaccgcg
     4381 ggcggtgggc tggcgccgaa ggctgcgcag cacctgccgg cgcagcgtcg tgtcgtcccc
     4441 gggcaggacc ggcgcggcgc tcacgagggc tctcccacga gccgcacgcg cgggtcgagc
     4501 gcggcgtaga ggaggtccac caggaggttc acgctcacga agaggaacgt cgccagcatg
     4561 atgtaggaga cgacgaccgg ccggtcgagc tggtagatgc tgtcgatgag gagcttgccc
     4621 attcccggcc acgcgaacac cgtctccgtg atggtcgaga acgcgatcag gctgccgaac
     4681 tcgatcccga ccaccgtcac cacggggatc aggatgttgc gcaggatgtg ccgtcccacg
     4741 atgcgccggg gccgcacgcc cttggcgcgc gcgtacttca cgtagtcctg tgccttcgcc
     4801 tccgtcgtgc ccgccgcgac gaggcggatc atgagggcca tgttcgggat cgcgaggttg
     4861 agcgccggca ggatcaggtg cctgaggccg tcgagggtga ggaggctcgt gcccacgccg
     4921 atgatctgca ccgtcgcgcc gcgtccggaa gtgggcagcc acccgagcag cacggagaag
     4981 acgaggatca gcatcatccc cttccagaag ttgggcagcg agtagcccag caccgtcccg
     5041 cccatgatga cgcgcgacag ccgcgcttcc gggttcagcc ccgcgaccag cccgagcggg
     5101 atgccgaagg cgagcgccag cgtcatcgcc accagcacca gctcgaaggt ggcgggcagg
     5161 cgcgagagga tgatctcgat ggccggcacg ccgtgcacga aggagcggcc gaggtccccg
     5221 tgcagcgcgt tccaggcgaa caccaggtac tgccgccaga gcggcaggtc gaggccgagc
     5281 cggcgcacga gctcgtcgcg ctcgagcgcg ctcgcctgcg gcggcacgag gagctccacc
     5341 gggtccccga tgccgtacac cgcgaggaac acgacgatcg aggccgcgag caggacgaag
     5401 aagctctgga tcagcctctg cagcgcgtac gtcgccatcg agggcgagtc cccctgccct
     5461 acttcttcgc cggcgccacc gacatggcga gcgtgtactg gtccgtgcgt cccttcacca
     5521 cgaggtccgc gcgatgggcc cagatggtgc tctcgaagtg cagcgggatg aaggcgcccg
     5581 tctccaggag ccgcgccacc gccaggcgca ggatgtccgc gcgcttgtcg tcgtccatcg
     5641 tgacgagggc cttgccgacg agctcgttga gcgccgcgtc gtcgaggccg ccgtagttcg
     5701 aggtgccgcg cgtcttcttc tcgtcgggag cggcggccca gtagcgcagg aacgaggagg
     5761 cctcggccgc gccccagccg cccatcgaga agctgaactc cttcttcgcg cgccgcggga
     5821 agtagatggc gcgcgtcatc gcgtccacct tcgtgcggat gcccacctgc gaaaggtacc
     5881 cggccacggc ctgcgcgatc tggccgtcgt tgatgtagcg atcgttgggc gtcgagagcg
     5941 tgatgtcgaa accgttcgcg tagcccgcct cggcgagcag cttcttcgcc gcggccggat
     6001 cgaacttgat gaccgacgga ttggcgagcg tgccgtacat gcccgtcggc aggaactggt
     6061 aggcgggatg cgcgccgccg tccatgaggc gcttggcgat cgcgtcccgg tcgatagcaa
     6121 tcgacatggc cttgcgcacg cgcgcgtcct ggaggggatt gccgcccttg tcgtccttca
     6181 cgagcgggct cgggctgcgc gccacgtcga gctggaagaa gatcacgcgc gaggatggcg
     6241 tgacgacgta ggcgaggcgc ttgtcctcct tgatgcggcc gaggtcgcgc gcggcggggt
     6301 tctcgacgac gtcgtagtcg cccgcgatga gccccgccag gcgcggcccg gcgttggtca
     6361 ccggcaccat cttcaccgcc tgccacggcg acttctcgcc ccagtagtcg tcgttgcgca
     6421 cgagctcgat cgccgagccg cgcacgtagc tcttcagcct gaacggcccg gtgccgatgg
     6481 cgaccgaccc ctcgttgaac tgggtcacgg agggccacgg gccggtcacg ccgcagttcg
     6541 cggcgagatt gaacgcgatc ttgccgtgcg ccgcgatgga ggcgctgagg atgccgatct
     6601 cggcgagcag ccggggcagc agcggctccg gctccgccgt cgtgacgacg agcgtgtacg
     6661 gatccggcgc ctcgaccgag cgcatgttgc ggatgatgag cttgttgccg ccggcgatgc
     6721 tggtctcgtt cttctccacg cggcagtagg tgaagatcgc gtcatccgcg gtgaagggct
     6781 tgccgttgga gaacttcacg cccttgcgca actggaaggt ccacttgttg gcgccgtcgt
     6841 tcttccagcc cgtggcaagc cccggcacca cgcgctgctg ctcgtccagg tcggtgagcg
     6901 cgtcgtagat gtgggcggcc agcgcgttgt tgggcgcgag gacgtggtaa tgcgggtcga
     6961 ccgaggtcgg ctccgaggaa agcgcgatgc gcagggtctg ggccccggcc gatgcggcgg
     7021 cgagcgcaag ggcggcggcc gcggcgagcg gaagcaggcg gaagcatttc atgggagcga
     7081 aacctcgtgg aaatcgtggg caggcaggtg ccgaatgacg tcggcgcggg acccttgatt
     7141 ctacggcatt gcccgatggg cattcgtgca agtggaggcg gtggctgtgg cggtggaagt
     7201 cgatcgcaat cgtgggcagg tgaggtgagg cggtggcggt ggaagtcgat cgcaatcctg
     7261 ggcacgcaat cgtgaccgtc ggatgggtcc caaaactccg aggccaccca ttagccggcc
     7321 cgtgtcaagg gcgaatcatc tcccgtcggc caaggtgggc gcctgggctg ctggggtcca
     7381 tatcgttgct gctcccggtc gagaccgttt cctcgattcg tggttacgac cgtgtcgttc
     7441 cagccaccca tctggatcaa gcggtttgcc ggtgccttgg cgccgccccg gcggcgaagc
     7501 cgccccagag aaacgttggt agtgccggga tgtcctttcg atgtttcctg caggtccgtg
     7561 gctcgcccga gggcgtccag tcctcgatgg gctcatccgg catacgaccc ggtttgcagc
     7621 aacgtggtgg taacggccgg cctccactca ggcagatggc tcgagtccag gcctcgactt
     7681 ggctgaccgg tcgtcacctt cgattcaggt aaaacagcgc ttcacgtcga aaggctgtcg
     7741 gtccttgagc acgtgatagg ccgcgcgagc cagcttgtgc gccagcgcct tggtggcgat
     7801 cacggtgttg gtgcgggcct tcttcctctc gaagaagcac ttggcctcgg cgctgaagcg
     7861 ccgggcgaag ttggccgcct cgacgaaggc ccacgccagg tagacgttac cgttcttcgt
     7921 gttgccttcg cccttcttct tgccgttcga gtagtgcgca ctgtccacgc agcgcgtata
     7981 ggaagcgaag ttgcccacgc ccgcgaagcg cccgatctcg cctgtctcga gcatgatcac
     8041 gatcgagagc acttccccga tgccggtgat gctcttgagc agcgcgtact ccggccgcgg
     8101 cttgaccgcc ttgagcagcc gcgtctccag aagcgcgatc tcctggttca aggtggcgat
     8161 caccgccacg ctcgacttca ccgccagcga aacgtctgcc cccagcccga gctgttccac
     8221 cgttgtggca ttcaagcgct ggatcgcctt gctcgccagc cgcgcgccgc gttcgcgcgc
     8281 caggatgttc tccaccgaca gcacgtgctg ggtgcgcgaa cgcacgagtt gggcgcgctt
     8341 gcgcgccaga tcccgcaggg cacgctgcgc cggcggatgg atgtagccgg tgggcaggat
     8401 tcccaggcgc agcaggtgcg ccaggaacgc ggcatcgtcc tcgtcgccgc tgtgcttgag
     8461 cccgtcatat cgcttcatgg ccacggtgtt ggcgagcttc acctcgtagc ccgccgcccg
     8521 cagcccgtcg accagccagt accagttgta ggtggattcg accaccacgc cagcgagttc
     8581 ttcccgatgg ggctcgagca tcgacaggat cgtcgccaga tcgttccgac agcggcggct
     8641 cagcaacttc cggtccgctt cgtccgtgac caccacgacg cagttgttcg aatgcagatc
     8701 tatgccacag aatctcatct cggcctcctt tgagtaaaac ggttggttcg cacctcgcgt
     8761 tttaccccac cgccctcggg cggtgggagg ccggctagat gatcctcagg cagaacaagc
     8821 caggcctcga ctttccaccc atctacagac gagggcgcaa acgctttctc gtccgcgacg
     8881 gtcgcgtgcc aaggattgcg atcgacttcc accgccaccg cctccacgct cagttgatac
     8941 agcagcgtgt gagagaacac cggatcgcag tgccatcgtt gctgactgcg ccacgctgcc
     9001 tcttcatggg ggccggggat ggggcaacac gaagaggcag cgtggcgcat tcggcagcgg
     9061 acgggacgcg gtccgtccgg cagcgcacga gaagtggtcc ggcgttgtct tactcggtgc
     9121 tgtatccacg gag
//
LOCUS       DASUEV010000354         7808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_540135, whole genome shotgun sequence.
ACCESSION   DASUEV010000354 DASUEV010000000
VERSION     DASUEV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540135"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFV55_11195"
     CDS             <1..735
                     /locus_tag="VFV55_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX4944910.1"
                     /translation="TKPLLTYGIRSDADLRAENVRWDAGRMRFRATGGPGPLEVTLNL
                     PGEHNVLNALAAIAVAREVGAPDDAIARGLAEFTGVGRRFQRYGEVVLASGERFALVD
                     DYGHHPAEMAATIAAVRGAFPGRRLLLAFQPHRFTRTRDLFEDFVRVLSSVDALVLAD
                     VYPAGEAPIVAADGRALARAVRVAGKVEPVFVEQVAQMAEAVLAAVQGGDVVVTMGAG
                     SVAGLAPELARPSIAVAGGESVRAST"
     gene            732..1676
                     /gene="murB"
                     /locus_tag="VFV55_11200"
     CDS             732..1676
                     /gene="murB"
                     /locus_tag="VFV55_11200"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX4944911.1"
                     /translation="MSDATRTQAPILRGELRRDDPMAKHVTWRAGGLARVSFRPADLE
                     DLQAFLASRPPEEPVLFVGLGSNLLVRDGGFDGAVVFTHRALTGIEFAPPLRHKLLFV
                     AGSGAPAPQLARFVARHGGAGAEWLAGIPGTVGGALAMNAGCYGGETWNHVVSVRTID
                     QRGVLRTRTAADYHIGYRQVVARAGAAEWFVGATFAFDPGEEAVAMARMKALLVRRVA
                     TQPLNKPNAGSVFRNPAGDHAARLIESCGLKGFTVGGAQVSTRHANFIVNLGPATAAD
                     IEAVIEHVQAAVRSRTGIDLVREVRIVGTAGTERKAAS"
     gene            1673..2596
                     /locus_tag="VFV55_11205"
     CDS             1673..2596
                     /locus_tag="VFV55_11205"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX4944912.1"
                     /translation="MTDRFGKVAVLMGGPSAEREISLISGKAVLGALLEKGVDAHAFD
                     PKERELFDLKREGYARAFIALHGRFGEDGTVQGALEVMGLPYTGSGVMASALSMDKWR
                     TKLVWLAAGIPTPRYAMVDAGTDWARLAVELGLPVVVKPAHEGSTLGLTIVRAAADLA
                     AAYAIAARFDPLVLAEEYVEGQELTASILGDEALPLIRIVAPQGNYDYQNKYFTDDTK
                     YHCPAGIRAGLEDEIRRAALASFRVLGCRGWGRADVMLRTDGTYSFLEMNTSPGMTGH
                     SLVPMAAKAVGISYADLCLRILEGAALETRP"
     gene            2593..3318
                     /locus_tag="VFV55_11210"
     CDS             2593..3318
                     /locus_tag="VFV55_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX4944913.1"
                     /translation="MKPAPAIALRAAGGLAAVALLAMVAWYGYDALARRPIATVRFTG
                     DTARVPAADLDRLATGLRGREAGDVALPAVREAVKRLPWVRDCVVRRVFPGALEVAIE
                     AHAPLARWDETRLVSVRGEVFTASFEGDLPRFSGPEGTAAEMAAAWKAIGAAAAPLAS
                     PVAEIRLSQRRAWQARLASGLTIELGRESIGERLARFAAVWPRIADSAATATHADLRY
                     PNGFALRAVAPEDRKPARKDRHA"
     gene            3341..4579
                     /gene="ftsA"
                     /locus_tag="VFV55_11215"
     CDS             3341..4579
                     /gene="ftsA"
                     /locus_tag="VFV55_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX4944914.1"
                     /translation="MNRSRESRTLLVALDVGTSKIVTLVAEVTPEMTLNLIGMGSHPS
                     RGLKKGTVVNIESTVTAIQRSLEEAELMADVKIRDVITGIAGSHIKSFNSHGMVAIKD
                     KEVSQYDIDRVIETARAVNIPMEQQVLHILEQEFIIDGQGGVRQPLGMSGMRLEVKVH
                     IVTGAVSAAQNIMKCVRRCGLEVRDLMLQPLASADAVLLDDERDLGVCLVDIGGGTTD
                     LAVFTEGAIKHTSVIPIAGDQITNDIAMALRTPTKDAEELKIRHGVALRQLASVNDMI
                     EVPGVGDRGAREISRQTLAEVIEPRVEELYSLVQRELRSCGLEELLSSGIVITGGTSL
                     MKGMVELGEEVFHMPVRVGLPQYAGALAEVVRNPRHSTGMGLLMAGLEQVKRDQHAKI
                     VGTGFRDVLEKMKNWFKGNF"
     gene            4644..5792
                     /gene="ftsZ"
                     /locus_tag="VFV55_11220"
     CDS             4644..5792
                     /gene="ftsZ"
                     /locus_tag="VFV55_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX4944915.1"
                     /translation="MIELLDAQSPEAVIKVVGIGGCGGNAVDHMINNGVQGVEFITLN
                     TDAQALKRNLARTTLQLGTGVTKGLGAGANPEVGRQAAEEDRSRIEELVGGADMLFIT
                     AGMGGGTGTGAAPVVASIAREMGILTVAVVTKPFDFEGKRQKVAAMGIEALKQHVDSL
                     IIIPNERLMQVLGEEVTYEDAFRASNDVLNGAVAGIAEVINCPGIVNVDFADVRTVMS
                     ENGVAMMGSAMASGPERAQMAAEQAVHSPLLEDIHLAGARGVLVNITASKSLKLKEVH
                     EVMNTIRSFTAEDATIIYGSVIEEAIGEHLRVTIVATGLGGAAAQRQPTISIVQRTGT
                     HDQPMAVNYEELEQPAAFRRRRDQTVDALRHSGMETLDIPAFLRKQAD"
     gene            6080..6994
                     /gene="lpxC"
                     /locus_tag="VFV55_11225"
     CDS             6080..6994
                     /gene="lpxC"
                     /locus_tag="VFV55_11225"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX4944916.1"
                     /translation="MILQRTLKTTVKATGVGVHTGHKVSLVLRPAAPDTGIVFCRSDL
                     PGNPAIPARAHNVVDTRMATVLEKDGARVATVEHLMSAFFGLGIDNAYVDVSAEEVPI
                     MDGSAATFVFLLQQAGVEEQPVPKKYVRVLKTLSIEQGDKRVSLSPFPGFKVGFSIEF
                     AHPVFEQSETTVDIDFGEVSFVKDVARARTFGFTQDVEAMRNAGLGRGGSLDNAIVVD
                     DFRVLNSEGLRFDDEFVKHKALDAIGDLYLLGHPLIGAFRGHKSGHALNNQLVRALIE
                     DASAWEEVTFAETRDLPAAFANIRLQPA"
     gene            6996..7181
                     /locus_tag="VFV55_11230"
     CDS             6996..7181
                     /locus_tag="VFV55_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944917.1"
                     /translation="MFVLRAVVLFALLFLLALGGAYLATRDRKYLRIAGKTIQFTTLL
                     AVAFALFYLFERVLLFL"
     gene            complement(7148..7642)
                     /locus_tag="VFV55_11235"
     CDS             complement(7148..7642)
                     /locus_tag="VFV55_11235"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX4944918.1"
                     /translation="MATEPLARLLRENADLAPVRNRLDQVKRLQGLYRSVVPVALASS
                     SRVCAVDGTTVVVRADNAPVAAALRAIAPRLLAGLNDPATAATGPPEKKPLKNKQDQE
                     LTALRVEIQVDVPAPPRKVRPRDDIPVERLAEVARGLSASPLKDALDRIVQAQRKSST
                     RSKR"
     gene            7665..7790
                     /locus_tag="VFV55_11240"
     CDS             7665..7790
                     /locus_tag="VFV55_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944919.1"
                     /translation="MNIIRVSDSLAKSRNVSLSQSQVVLVALGILMSGFFLAMAT"
BASE COUNT         1243 a         2623 c         2741 g         1201 t
ORIGIN      
        1 acgaagccgc tgctcaccta cggcatccgc tcggacgcgg acctgcgtgc cgagaacgtg
       61 cgctgggacg cggggcgcat gcgcttccgc gcgaccggag gccccgggcc cctcgaggtc
      121 acgctgaacc tcccgggcga gcacaacgtc ctcaacgcgc tcgcggccat cgccgtcgca
      181 cgcgaagtgg gcgcgccgga tgatgcgatc gcccgggggc tcgccgagtt caccggcgtc
      241 ggccggcgct tccagcgcta tggcgaggtg gtcctcgcct cgggcgagcg cttcgcgctc
      301 gtcgacgatt acggccatca cccggccgaa atggcggcga ccatcgccgc cgtgcgcggc
      361 gcctttcccg gccgccgcct gctgctcgcg ttccagccgc atcgcttcac gcgcacgcgc
      421 gacctgttcg aggacttcgt gcgcgtgctc tcgagcgtgg atgcgctggt cctggcggac
      481 gtgtatcccg ccggcgaggc ccccatcgtg gccgccgacg gacgcgcgct ggcgcgcgcc
      541 gtgcgcgtcg ccggcaaggt ggagccggtg ttcgtcgagc aggtcgccca gatggcggaa
      601 gcggtgcttg ccgccgtgca gggcggcgat gtcgtggtca ccatgggcgc cggttcggtg
      661 gccggcctgg cgccggaact cgcgcgcccg tcgatcgccg tggcgggggg cgaaagcgtg
      721 agggcatcga catgagcgat gcgacccgga cgcaggcccc gatccttcgc ggcgaattgc
      781 gccgtgacga tccgatggcg aagcacgtga cctggcgcgc gggcggcttg gcgcgggtgt
      841 ccttccgccc cgccgacctc gaggacttgc aggctttcct cgcgtcgcgc cccccggagg
      901 agccggtgct cttcgtcggg ctcggcagca acctgctggt gcgcgacggc ggcttcgacg
      961 gcgccgtggt cttcacccac cgcgcgctca ccggcatcga gttcgccccg cccctgcggc
     1021 acaagctgct cttcgtcgcg ggctcgggcg cgccggcccc gcagctggcg cgcttcgtgg
     1081 cgcggcacgg cggcgccggt gccgaatggc tggcgggcat acccggcacg gtgggcggtg
     1141 cgcttgcgat gaacgcgggc tgctacggcg gcgagacctg gaaccacgtt gtgtcggtgc
     1201 ggaccatcga ccagcgcggc gtgctgcgca cgcgcacggc cgcggactac cacatcggct
     1261 accggcaggt cgtggcgcgc gccggcgcgg cggaatggtt cgtcggcgcc acgttcgcct
     1321 tcgatccggg cgaagaggcg gtggcgatgg cgcgcatgaa ggcgctgctc gtcaggcgcg
     1381 tggcgaccca gccgctcaac aagcccaacg cgggcagcgt cttccgcaac ccggccggcg
     1441 accacgcggc gcgcctgatc gaatcctgcg ggctcaaggg attcacggtc ggcggcgcgc
     1501 aggtgtcgac gaggcatgcc aacttcatcg tgaacctcgg accggcgacg gcggccgaca
     1561 tcgaagcggt catcgagcat gtgcaggcgg cggtgcgcag ccgaacgggg atcgatctgg
     1621 tccgggaagt tcgtatcgtc ggtaccgccg gcaccgagag gaaggccgca tcgtgaccga
     1681 ccgcttcgga aaagtcgcgg ttctcatggg cggcccgtcg gccgagcgcg agatctcgct
     1741 catctccggg aaggcggtcc tcggggcgct gctcgagaag ggcgtggacg cccatgcctt
     1801 cgacccgaag gagcgcgagc tcttcgacct gaagcgcgaa ggctacgcgc gcgccttcat
     1861 cgcgctgcac ggccgcttcg gcgaggacgg cacggtgcag ggagcgctcg aggtgatggg
     1921 gctgccctac accggcagcg gtgtcatggc ctccgcgctt tcgatggaca agtggcgcac
     1981 gaagctcgtg tggctggccg ccggcatccc cacgccgcgc tacgcgatgg tggatgcagg
     2041 caccgactgg gcgcgcctcg cggtcgagct gggcttgccg gtcgtcgtga agcccgcgca
     2101 cgaggggtcc acgctgggcc tcaccatcgt tcgcgcggcg gcggacctgg ctgccgcgta
     2161 tgcgatcgcc gcgaggttcg acccgctcgt gctcgccgag gagtacgtcg agggacagga
     2221 gctcaccgcg agcatcctgg gcgacgaggc gctgccgctc atccgcatcg tggcgccgca
     2281 gggcaactac gactaccaga acaagtactt cacggacgac acgaagtacc actgcccggc
     2341 gggcatccgc gccggcctcg aggacgaaat ccgccgcgct gcgctcgcca gcttccgcgt
     2401 gctgggctgc cgcggctggg gacgcgcgga cgtgatgctg cgcaccgacg ggacgtactc
     2461 gtttctcgag atgaacacgt cgcccgggat gacgggccac agcctggtgc cgatggcggc
     2521 gaaggcggtg ggcatctcct acgcggacct gtgcctgcgc atcctcgaag gcgcggcact
     2581 ggagacgcgg ccgtgaagcc ggcgccggcc atcgccttgc gcgccgcggg cgggcttgcc
     2641 gccgtggcct tgctggccat ggtggcctgg tacggctacg acgcgctcgc ccgccgtccc
     2701 atcgccacgg ttcgcttcac cggggacacg gcccgggtgc cggccgcgga cctcgaccgc
     2761 ctggcaacgg gattgcgcgg acgcgaggcc ggcgatgtgg cgctgccggc tgttcgcgaa
     2821 gccgtgaaac gcctgccctg ggtgcgcgat tgcgtggttc ggcgcgtgtt ccccggggcc
     2881 ctcgaggtgg ccatcgaggc gcatgcaccg ctcgcccgct gggacgaaac gcggctcgtg
     2941 agcgtgcggg gcgaggtctt caccgccagt ttcgaaggcg acttgccgcg cttctccggc
     3001 ccggagggca cggctgcgga gatggccgcg gcgtggaagg ccatcggggc agccgcggcg
     3061 ccgctcgcga gcccggtcgc ggaaatccgc ctttcccagc gccgcgcgtg gcaggcaagg
     3121 ctcgcctcgg gcctcaccat cgagctcggg cgcgaaagta tcggggagcg gctcgcccgt
     3181 ttcgcggccg tgtggccgcg catcgccgac tcggcggcaa cggcgaccca cgcggacctg
     3241 cgctacccga acggcttcgc gctgcgcgca gtggcacccg aggaccgcaa gccggcgcgg
     3301 aaggaccggc acgcatgacg aagaaggcga cgacatgatc gtgaaccgca gccgcgagag
     3361 ccgcaccctg ctggtggccc tcgacgtcgg cacctcgaag atcgtgaccc tggtggccga
     3421 ggtgacgccc gagatgacgc tcaacctcat cggcatgggc agccacccct cgcgcggcct
     3481 caagaagggc acggtggtga acatcgagtc cacggtgacc gccatccagc gctcgctcga
     3541 ggaagccgag ctgatggccg acgtgaagat ccgcgacgtg atcaccggca tcgccggcag
     3601 ccacatcaag agcttcaact cgcacggcat ggtggcgatc aaggacaagg aggtgtcgca
     3661 gtacgacatc gaccgcgtga tcgagaccgc gcgcgcggtg aacatcccga tggagcagca
     3721 ggtacttcac atactggaac aggaattcat catcgacggg cagggcggcg ttcgccagcc
     3781 gctcggcatg agcggcatgc gcctcgaggt gaaggtgcac atcgtgacgg gcgcggtgtc
     3841 cgcggcgcag aacatcatga agtgcgtgcg ccgctgcggg ctcgaggttc gcgacctcat
     3901 gctgcaacct cttgcctcgg ccgacgccgt cctcctggac gacgagcgcg acctgggcgt
     3961 gtgcctcgtg gacatcggcg gcggcacgac ggatctcgcc gtgttcaccg agggcgccat
     4021 caagcacacg agcgtgatcc ccatcgcggg cgaccagata accaacgaca tcgcgatggc
     4081 gctgcgcacc ccgaccaagg acgccgagga gctgaagatc cgccacggcg tggcgctgcg
     4141 ccagctcgcc agcgtgaacg acatgatcga ggtgccgggc gtgggcgatc gcggcgcgcg
     4201 cgagatctcg cgccagacgc tcgcggaagt gatcgagccg cgcgtcgagg agctctactc
     4261 gctggtgcag cgcgaactgc gctcctgcgg gctggaggag ctcctttcct ccgggatcgt
     4321 gatcaccggc gggacctcgc tcatgaaggg catggtcgag ctgggagagg aagtgttcca
     4381 catgccggtg cgcgtggggc tcccgcagta cgccggtgcg ctggccgagg tggtgaggaa
     4441 cccgcgccac tcgacgggga tgggattgct catggcgggg ctggagcagg tgaagcgcga
     4501 ccagcacgcg aagatcgtgg gcacggggtt cagggacgtg ctcgagaaga tgaagaactg
     4561 gttcaagggg aatttctagg atcggcaggc aggcggaagg cggcaggccg ggtaactgca
     4621 gtcacaacta caggagacga cacatgatcg aattgctgga tgcccaatcg ccggaagcgg
     4681 tgatcaaggt ggtgggaatc ggcggttgcg gcggcaacgc cgtggaccac atgatcaaca
     4741 acggcgtgca gggcgtggag ttcatcaccc tgaacaccga cgcgcaggcc ctcaagcgca
     4801 acctggcgag gaccacgctg caactcggca ccggcgtcac gaagggactt ggcgcgggcg
     4861 ccaaccccga agtgggccgc caggcggcgg aggaggacag gagccgcatc gaggaactgg
     4921 tgggcggcgc cgacatgctc ttcatcacgg cgggcatggg cggcggcacg ggcacgggcg
     4981 cggcgccggt cgtggcttcc atcgcgcgcg agatgggcat cctcacggtg gccgtggtca
     5041 ccaagccctt cgacttcgag ggcaagcgcc agaaggtggc ggccatggga atcgaggcgc
     5101 tcaagcagca cgtcgattcc ctcatcatca tccccaacga acgcctgatg caggtgctgg
     5161 gcgaggaagt cacctacgag gacgccttcc gcgcctccaa cgacgtgctg aacggcgccg
     5221 ttgcgggcat cgcggaggtg atcaattgcc ccggcatcgt gaacgtggac ttcgccgacg
     5281 tgcgcaccgt catgagcgag aacggcgtgg cgatgatggg ctcggcgatg gcttcgggac
     5341 cggagcgcgc ccagatggcc gcggaacagg cggtgcattc gccgctgctc gaggacatcc
     5401 acctcgccgg cgcccgcggc gtcctggtga acatcaccgc ctcgaagagc ctcaagctca
     5461 aggaggtgca cgaagtgatg aacaccattc gctcgttcac cgccgaggat gcgaccatca
     5521 tctacggcag cgtgatcgag gaggcgatcg gcgagcacct gcgcgtgacg atcgtggcga
     5581 cggggctcgg cggcgccgcc gcccagcgcc agccgacgat ctcgatcgtg cagcgcaccg
     5641 gcacccacga ccagccgatg gccgtcaact acgaggaact ggagcagccc gccgcgttcc
     5701 ggcgacggcg cgaccagacg gtcgacgcgc tgcgccattc cggcatggaa acgctcgaca
     5761 tccccgcctt cctgcgcaag caggcggact gacgtcacgg gacgaagttg cgtccccgat
     5821 cgtcgttccc gcgaaggcgg gaatcgatgg cacacgcgac cgccgctgtc tcccggcggc
     5881 cgtgcccgcc ttccttcggg cccgcgaaag cgggcccttt tcacgtctgc caggagcgtt
     5941 ggggagtttg tcgaaatccg gcctcctgtc ttcattcttc cccgcccgat cggaagccga
     6001 tgggaaattc cgctaacgcg atgatagaat tacgcttttg gatgcgccgg gcggccacga
     6061 gcggccacgg ggcgacccca tgatcctgca gcgcacactc aagaccaccg tcaaggcgac
     6121 cggcgtcggt gtccataccg gccacaaggt gtcgctcgtc cttcgccccg ccgccccgga
     6181 taccggcatc gtcttctgcc gttcggacct tccggggaat ccggccattc cggcacgcgc
     6241 gcacaacgtg gtggatacgc ggatggccac cgtcctcgaa aaggatggcg cacgcgtggc
     6301 tacggtcgag cacctgatga gcgcgttctt cgggctggga atcgacaacg cctacgtcga
     6361 cgtttccgcc gaggaagtgc cgatcatgga tggcagcgcc gccaccttcg tgtttctcct
     6421 gcagcaggcc ggcgtcgagg aacagccggt cccgaagaag tacgtccggg tcctgaagac
     6481 cctctcgatc gagcagggcg acaagcgcgt gagcctttcg cccttcccgg ggttcaaggt
     6541 cggcttttcg atcgaattcg cccacccggt gttcgaacag tcggagacga ccgtcgacat
     6601 cgacttcggc gaggtctcgt tcgtgaagga cgtcgccagg gcccgcacct tcggcttcac
     6661 ccaggatgtg gaggccatgc gcaacgcggg cctaggtcgc ggcggctccc tggacaacgc
     6721 catcgtggtg gacgatttcc gggtcctcaa cagcgaaggc ctgcgcttcg acgacgagtt
     6781 cgtgaagcac aaggcgctcg acgccattgg cgacctctac ctcctcggcc acccgctcat
     6841 cggcgccttc cgcgggcaca agtcggggca cgcgctcaac aaccagctcg tgcgcgccct
     6901 catcgaggac gcatccgcct gggaagaggt gaccttcgcg gaaacgcgcg acctgccagc
     6961 cgcattcgcc aacatccggc tgcagcccgc ctgacttgtt cgtcctccgg gcggtcgtcc
     7021 ttttcgcgct cctcttcctg ctcgcgctgg gtggggccta cctcgccacg cgcgaccgga
     7081 agtacctgcg gatcgcggga aagaccatcc agttcaccac gcttctggcg gtcgccttcg
     7141 cccttttcta cctcttcgag cgggtgctgc tcttcctctg agcctggacg atccggtcga
     7201 gggcgtcctt gagcggcgac gcggatagcc ccctggccac ctcggccagc cgctcgactg
     7261 ggatatcatc ccggggacgg accttgcggg gcggcgcggg gacatcgact tgaatttcca
     7321 cccgcaatgc agtaagctct tgatcctgtt tgttttttaa gggttttttc tccggcgggc
     7381 cggtcgcggc agtggcggga tcgttcagcc cggcgagcag tctcggcgcg atcgcccgca
     7441 gggcggcggc gaccggcgca ttgtctgcgc gaaccacaac agtcgttcca tcaacggcgc
     7501 atacccggct cgaggaagcg agcgctacgg gtaccacgga ccgatagagt ccttgcaggc
     7561 gcttgacctg atccagccgg ttgcgaaccg gggccaggtc ggcgttttcg cgtagcagtc
     7621 gggcgagcgg ttcggtggcc atcgttggcg acgaggagtg acggatgaac attattcggg
     7681 tttcggacag cctggcaaaa agccgcaatg tctcgctgtc ccagtcccag gttgtcctgg
     7741 tggcgctggg catcctgatg tcgggcttct tcctggcgat ggcgacctaa gtcgtcacga
     7801 tgaagttc
//
LOCUS       DASUEV010000355         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_543009, whole genome shotgun sequence.
ACCESSION   DASUEV010000355 DASUEV010000000
VERSION     DASUEV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543009"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFV55_11245"
     CDS             <1..632
                     /locus_tag="VFV55_11245"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX4944920.1"
                     /translation="YAQLASRAYDRALQLDKNNTTAQVKLAMVKDLFVSPKTAAAARR
                     DPEKVASAGQARAEPPKVETAKVESAKVKPGKVEPAKVESAKPAPAAIVPAADDKGDV
                     GAAIEAWARAWSTKDVKAYLAAYAPDFETPRGEARDAWEKQRTERIQRPKSIEVTVKI
                     QSITIGGGSATAVLRQSYRSDALKTTSTKTLKLAKVGGKWLIKQERVGG"
     gene            686..1864
                     /locus_tag="VFV55_11250"
     CDS             686..1864
                     /locus_tag="VFV55_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX4944921.1"
                     /translation="MALLLATAVPSATVADGALPHGTLPAFANDIEASLVRTIVGLRE
                     SGLRRALGEIDMALAKTPNFRLGHLIRGDLLMARAGIPVAFGPLSTSSSAAMAPLQEE
                     ARARLKHYLDAPPPDHLPLPMMLLGPAQAHAILVDTARSRLFVFANHGGEPRYVTDFY
                     ISLGKNGVEKRREGDQKTPLGVYTIVSSREKLPDFYGPGAFPISYPNEWDRQHGRNGH
                     GIWLHGTPSGTYSRPPFATDGCVVLTNEDLRRLARYVDVGRTPVVISAGIDWVPPDRW
                     QASRHDFLAAFEQWRTDWESLDADRYLSHYSPRFRSDGKDFAAWGAHKRKVNAGKSWV
                     KVDVTDLSVFGHPDGEDMVVVTFAQDYRSSNLSNRTVKRQYWSRDQGRWRVVFETVIS
                     "
     gene            2028..2522
                     /locus_tag="VFV55_11255"
     CDS             2028..2522
                     /locus_tag="VFV55_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX4944922.1"
                     /translation="MKVKLTTSMGAITVELNKEKAPVSAENFVKYVESGHYNGTVFHR
                     VIDGFMIQGGGFTKDMQQKPVRAPIKNEAANGLKNDNYTLAMARTNVRDSATAQFFIN
                     VKANDFLNYRDESPQGWGYAVFGKVVEGQDVVDKIRKVPTGNAGGHQNVPLEPVTIEK
                     AECL"
     gene            2583..3305
                     /locus_tag="VFV55_11260"
     CDS             2583..3305
                     /locus_tag="VFV55_11260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX4944923.1"
                     /translation="MPRTLFISDLHLAEERPATTERFARFLADCVPGADALYILGDLF
                     EYWVGDDGMVLDFPARIAGLLRPAARSAPTFFMHGNRDFMVASRFCAETGIRLIPDPT
                     VIDLYGERALLMHGDTLCTGDHAYLAFRKQVRNPAWQAAALVRPIEERIAIARGMREK
                     SDGAKGGKGEAIMDVAPEAVEAAFASADCRLLIHGHTHRPARHEHSAGGRMCVRWVLP
                     DWYEAGGYLEASPAGIRAVAVA"
BASE COUNT          608 a         1236 c         1099 g          528 t
ORIGIN      
        1 tctatgcgca gctcgcgagc cgcgcctacg accgcgcgct ccagctcgac aagaacaaca
       61 ccacggcaca agtgaagctc gccatggtga aggatctctt cgtttcgcca aaaacggcgg
      121 ccgctgcccg gcgggacccc gagaaggtcg ccagtgcagg gcaagccagg gccgagccgc
      181 cgaaggtcga gacggcgaag gtcgaatccg ccaaggtcaa gccggggaaa gtcgagccgg
      241 ctaaggtcga atccgccaag cccgcgcccg ccgcgatcgt accggccgcg gacgataagg
      301 gcgacgtcgg cgccgcgatc gaagcgtggg cccgggcatg gagcacgaag gacgtgaagg
      361 cctatctcgc cgcctatgcg cccgatttcg agaccccccg cggcgaggct cgcgacgcct
      421 gggagaagca acgcaccgag cgcatccagc ggcctaagtc gatcgaggtg acggtgaaga
      481 tccagtccat cacgatcggc ggcgggtccg cgaccgcggt cctgcgccag tcctacaggt
      541 cggacgctct caagaccacc agcacgaaga cactcaagct ggcgaaggtc ggcggcaagt
      601 ggctgatcaa gcaggaacgc gtaggcgggt gacggaccga gcgcaccggc ccgcccctgg
      661 ccgcgccctg gcccgcgcgg gcgccgtggc cctgctgctc gccacggccg tgccttcggc
      721 cacggtcgcc gacggcgccc tgccccatgg gacgctcccc gccttcgcga acgatatcga
      781 ggcctccctc gtgcgcacga tcgtcggcct tcgcgagagc ggccttcgcc gcgcgctcgg
      841 cgagatcgac atggcgcttg cgaagacgcc caacttccgg ctggggcacc tcatccgggg
      901 cgacctcctg atggcccgcg ccggcatccc cgttgcgttc ggcccgctct cgacctcgtc
      961 cagcgcggcc atggcaccgc ttcaggagga ggcccgcgcg cgcctgaagc attacctgga
     1021 cgcgccgccg cccgaccatc ttccgctgcc gatgatgctg ctgggtcccg cgcaagccca
     1081 cgcgatcctc gtggacacgg cccggtcgcg gctcttcgtt tttgccaacc acggcggcga
     1141 accccgctac gtgaccgact tctacatcag tctcggcaag aacggcgtcg agaagcgccg
     1201 cgaaggcgac cagaagacgc cactcggggt ctacaccatc gtttcgtcca gggagaagct
     1261 ccccgatttc tacgggcccg gcgcgttccc gatcagctac ccgaacgagt gggacaggca
     1321 gcacgggcgc aacggtcacg gcatctggct gcacggcacg ccctccggga cctacagccg
     1381 cccgccattc gccacggacg ggtgcgtggt gctcaccaac gaggaccttc gccgactcgc
     1441 gcgttacgtc gacgtcggcc gcacgcccgt cgtcatcagc gccgggatcg actgggtgcc
     1501 gccggatcgc tggcaggcga gccgccacga tttcctcgcc gccttcgagc agtggcgcac
     1561 cgactgggaa agcctcgatg cggaccgcta cctgtcccac tactcccccc gcttccgctc
     1621 ggacgggaag gatttcgccg cctggggcgc gcacaaacgc aaggtgaacg ccggcaagtc
     1681 gtgggtgaag gtggacgtaa ccgacctgag cgtcttcggc catcccgacg gcgaggacat
     1741 ggtcgtcgtg accttcgcac aggactaccg atcctcgaat ctctccaatc gcaccgtcaa
     1801 gcgccagtac tggtcccgcg atcagggccg gtggcgcgtc gtcttcgaaa ccgtcatctc
     1861 ctgagaacgc cgccatgaaa ccgatgaata ccgcacgtcg ccttcttgcc gccaccctcc
     1921 tcctcgccat ttccggcgtg cctcttgcag cctccgcaca ggcgaaggaa aagcccgacg
     1981 cagccgcgcc cgccggatgt tccgccaacc tgaaaggatc cgccccgatg aaagtgaagc
     2041 tcaccacctc gatgggtgcc atcaccgtcg aactgaacaa ggaaaaggcg cccgtctccg
     2101 ccgagaactt cgtcaagtac gtggagtcgg gccactacaa cggcaccgtc ttccatcgcg
     2161 tcatcgacgg attcatgatc cagggcggcg gcttcaccaa ggacatgcag cagaagccgg
     2221 tccgggcgcc gatcaagaac gaggccgcca acggcctgaa gaacgacaac tacacgctcg
     2281 ccatggcgcg cacgaacgtg cgcgactcgg ccaccgcgca gttcttcatc aacgtgaagg
     2341 cgaacgactt cctcaactac cgcgacgagt cgccccaggg ctggggctat gccgtgttcg
     2401 gcaaggtggt cgagggccag gacgtggtcg acaagatccg caaggttccg acgggcaacg
     2461 ccggcggcca ccagaacgtg ccgctggagc ccgtgacgat cgagaaggcc gagtgcctct
     2521 gattttcgcg aggcgctgaa cacggaaagg gcggccccgg ggtcgccctt cgttttgccg
     2581 ccatgccgcg cacccttttc atttccgacc tgcacctcgc cgaggaacgg ccggcaacaa
     2641 cggagcgctt cgcccgtttc ctcgccgact gcgttcccgg cgccgatgcg ctttacatcc
     2701 tcggcgatct cttcgagtac tgggtgggtg acgacggcat ggttctcgac tttcccgcgc
     2761 gcatcgcggg actgctccgc ccggccgcgc gctccgcgcc cacgttcttc atgcatggca
     2821 accgcgactt catggtggcc tcgcgattct gcgcggagac gggcatccgc ctgattcccg
     2881 acccgacggt gatcgacctg tacggggagc gcgcgctcct catgcacggc gacacccttt
     2941 gcaccggcga ccatgcctac ctcgccttcc gcaagcaggt tcgcaatccc gcgtggcagg
     3001 ccgccgcgct cgtgcgcccg atcgaggagc gcatcgcgat cgcgcgcggc atgcgcgaga
     3061 agagcgatgg cgccaagggc ggcaagggcg aagcgatcat ggacgtggcc cccgaggccg
     3121 tcgaggcggc cttcgcgtcg gccgattgcc gcctgctgat ccatggccac acgcaccgtc
     3181 ccgcacgcca tgagcattcg gccggcggcc gcatgtgcgt gcgctgggtg cttccggact
     3241 ggtacgaggc gggcggttac ctcgaagcgt cacccgcggg aatccgcgcc gtcgccgtcg
     3301 cctgaactcg ggcgaggata taacccggac catcgatccc ctgcggtgac gagcgcatcg
     3361 acacgccgat aaggacgacg acgttcgggg gcacgttggt tgtgttttat ttcgtcttcg
     3421 gccaggtcaa tttgcgtctg gcgtcacgag tcgagcctat gtcgaagcgc c
//
LOCUS       DASUEV010000356        11453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_543664, whole genome shotgun sequence.
ACCESSION   DASUEV010000356 DASUEV010000000
VERSION     DASUEV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543664"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="VFV55_11265"
     CDS             complement(<1..1972)
                     /locus_tag="VFV55_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944924.1"
                     /translation="MNVITRWIVWGLLVAGRVFAIALLGLLCAYEALALNISVVGSDG
                     LAVTNYRWLVQEDMTKASVPGQPAGAGNLSLNFHTSYMPVVMTGNGANPVAALDPTKR
                     YYLSILPQSGYQMGGTPIAPGQTSASVWVNTQPIPTAQISVFVFEDNQPINGAPNLPQ
                     ELGLGGFTVTLIEAGGTYGQSGGQVTKDAFGNPLGTTYQQLPNGDFVFNPDGSPAVLA
                     LGNGVIISGPDGTARIKNLYPAKYTITVSPPAGSDWHQTSTLEGTKGIDAWVKANEPP
                     YFQEFGPPGHHVFMGFVRTLNAANVLNGPTTITGRVVNIHNSRPPDYAFFNGAPFPQC
                     WVGLNEMPAVGGRGVFTKACNADSTFSIPNVSPGQYQLVIWDDNLDMIISTSNVTVPA
                     GVNTVNLQEVPVFNWYGRFESRVFFDTNQNGFRDAGEAPIPGQNINLRFRDGSIYQSY
                     PTNPQGLAVFNEVFPFFNWLIAEVDFARFKATGATIVVDAGGPVPPHDGWTMPSFDRL
                     NPQPQSENAGLPYRTETGPVLLEGMQLFLGQTNVIEWGKSTYAPGENGGITGIVRYAT
                     TRAEDDPRYATAENWEPGIPRVQVNLYRDCNADGIIDVPDLTPGSSACLSLGSAAAPP
                     TLADVDNYPFGWSEGGSQGPEDVKRNSNPGFSG"
     gene            complement(2193..2858)
                     /locus_tag="VFV55_11270"
     CDS             complement(2193..2858)
                     /locus_tag="VFV55_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX4944925.1"
                     /translation="MATHWRIVIVEDHTLFRTGLRALLAKDADLEVVGEADNGRDAFR
                     VCSTLKPDLVLMDISMPGMNGIEAIAEIRRREPETRVIVLTIHKTDEYIHEALRAGAN
                     GYVLKDATHDELRTAIRTVLGGKTFLSPEISGRVISTYLGALRAPLTIRPVDMLTQRE
                     RQILKLVAEGHHNKEIAEFLSLSPKTVEKHRSNLMKKLDVHSIAALTAFAIANGLLGS
                     ANS"
     gene            complement(2878..4095)
                     /locus_tag="VFV55_11275"
     CDS             complement(2878..4095)
                     /locus_tag="VFV55_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4944926.1"
                     /translation="MGTIGSLFRASLRFSAREAFAENATGKPAAAVAGTARANHDSRV
                     LRCTTLALHRSEQRLRVAAAVMDEALLIMDRQKAIVFASAKFAALSGCSTDDLVGRPL
                     LGLFDRSERDDIRARFAALSPNHRARYGATLVRGDGRPLPVDVTFERIAAQDDVGPVW
                     WAVLREAPPLVKVPVQPPCDDARRLSCAMQAAQESERKRIAADLHDGLGQMLSAVKFG
                     LQTASGAIGDNETGQARETIDGLVSTVKAALEEVRRLSMNLRPSTLDDLGLVATLTWF
                     VREFESIYRDISVEVEMLAAETDIDEALRLPVFRIVQEAMNNVAKHSHATRVRVRLAR
                     DAELLRLTIADNGVGFDPVAVEARKGLERRCGHCCSRERAESCGGTFAVEAAPGRGVT
                     VSATWRLHPAAGS"
     gene            complement(4248..5591)
                     /locus_tag="VFV55_11280"
     CDS             complement(4248..5591)
                     /locus_tag="VFV55_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX4944927.1"
                     /translation="MKGFLRNYSMASLAGIFIAAVAIFVMYRAIVIRSVTTIAEGSGV
                     TVARLALHPIRPYLVEYFEVAGAAGRDPLSVAMPEALQDAIADMMRDTQIARIKIYNR
                     GGLVLFSTESRQIGNDQSANQGFRTAMGGKVSARLVYRDSFNPFDQVTEDDNLVQTYI
                     PVRRRPTEPVAGVIEMYTSVDALVAEAESSELQMLAVMALILTGLYAALLLLVYRSHE
                     LIETQQRTIREKNALLEQLSRENLRREESERKKFATDLHEGLAQTLSAVKLAVENARG
                     DTGPDGTSSLESVIPGLQSAIGQVRTIAMDLRPSGLDDFGLVPTLEALCADFLEATPE
                     FRVARQFTAHETDIPASLKIVVYRIVETALKIVAGHPGATGMRIGLEASNRMLTLVIE
                     DDADAVVAAMSDESGARDSESAFMAARERAIISGGKLSVVRNASGNPMLRVSWRY"
     gene            6731..7588
                     /locus_tag="VFV55_11285"
     CDS             6731..7588
                     /locus_tag="VFV55_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944928.1"
                     /translation="MRIDVCAIVLKLNAQFAAGLPLPAYCANPSASFNATGTFQTMTI
                     PVTGNYLIEAAGAQGGADSLTYAHAGGLGARVSGIVPLAAGDVLCIIVGMRPAGPAGP
                     YDAGGGGGGSFVFRSDAGCTAKPALPLIVAGGGGGGAGSGGEVTVNGGNGAGLGGVNG
                     AGGSAYISGLSEYHSGGGGAGWSSLGQGGSPATFAGGGGHWAGGAGASYLNTLSSPGG
                     FGGGGGAGMVCEAGCGSGGGGGFSGGGGGARTTDLHAGGGGSYISGTSPVSTTAVQAG
                     HGYVAITLQ"
     gene            complement(7612..9237)
                     /locus_tag="VFV55_11290"
     CDS             complement(7612..9237)
                     /locus_tag="VFV55_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX4944929.1"
                     /translation="MRKPFAITLDPGSSLANHTGSWRTSRPEYVDRLPPCNNACPAGE
                     NIQAWLFHAESGDYQAAWRSLVQNNPLPGVMGRVCYHPCESSCNRAQLDSSVGINSVE
                     HFLGDEALKRGWKFDPPAPATGKRVLVVGAGPSGLSAAYHLARLGHAVTIHEAGPMAG
                     GMMRFGIPKYRLPRDVLDGEVQRILDLGVKLEVNAKVDDVLASMKAGGFDAAFLAVGA
                     HIAKRAYIPAGEAAQIMDAVQVLRGMEGEEKPLLGRRVVVYGGGNTAIDVARTARRLG
                     AEEAMIVYRRTREKMPAHDFEVQEALDEGVLIKWLSTIKQAGEGSITVERMELDAKGV
                     PQPTGELEKLEADSVVLALGQDVDLSLLDRVPGMEIADGVVKVGPNMMTSHPGIFAGG
                     DMVPAERTVTVAIGHGKKAARHIDAWLRGGTHDPGPKHELAGFGKLNTWYYSDASKTV
                     RPILDMQKRVTSFDEVVMALDEGTALFEARRCLSCGNCFECDNCYGVCPDNAVVKLGP
                     GNRFRFNYDYCKGCGICATECPCGAIRMVPEEI"
     gene            complement(9234..10229)
                     /locus_tag="VFV55_11295"
     CDS             complement(9234..10229)
                     /locus_tag="VFV55_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX4944930.1"
                     /translation="MSFQPVKFYQTGTFTVGNRLLPDAERSVQSTVRRHNSLNSGHRA
                     CQGCGEALGARYAIDAAMEATRGQLIAANATGCLEVFSTPYPETSWQIPWIHSLFGNT
                     AAVATGIAAAMRVKGRDNVRVVAQGGDGGTTDIGFGCLSGMFERNDDVLYICYDNGAY
                     MNTGVQRSSATPGAARTATTAAVGPSPGNPFGQGKDLPTIAIAHGIPYVATATVSELR
                     DLEAKVRRAMQFRGARYLHILVPCPLGWGHDSSLTIRMARLAHETGIFPVFEAENGEV
                     TGVAKIRRQVPVEEYLKPQKRYAHLFSPKPREDVIRHIQENADRNIRKYRLLEEA"
     gene            complement(10240..>11453)
                     /locus_tag="VFV55_11300"
     CDS             complement(10240..>11453)
                     /locus_tag="VFV55_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX4944931.1"
                     /translation="VALCRPEVICAYPISPQTHIVEELGEMVKSGELAPCEFINVESE
                     FAAMSVAIGASAAGARAYTATASQGLLFMAEAVFNASGMGLPIVMTVANRAIGAPINI
                     WNDHTDSMSQRDCGWIQLFAETNQEALDLHIQAFRLAEELSLPVMVCMDGFILTHAYE
                     RVDVPTQEQVDAFLPPFDPRQVLDPQRPVSMGAMVGPEAFMEVRYLAHAKQMQALELI
                     PAWAAEFRDAFGRSSGGLTQTYRTEDADTIVVALGSVLGTIKETVDALREKGHRIGVL
                     GITCFRPWPLAAVRDALANAKCIVVLEKCLAVGMGGIVSANIRVSHRGPPESVHTVIA
                     GLGGRQITRDSLARAFVEAEEGVLPPLTFLDLNRPVVDRALARERESRRTGPLAEALL
                     RDVGLVTAQLR"
BASE COUNT         1763 a         3814 c         3994 g         1882 t
ORIGIN      
        1 ccccgctgaa gccggggttg ctgttgcgct tcacgtcttc cgggccctgc gagccgcctt
       61 ccgaccagcc gaagggatag ttgtccacgt cggcaagcgt cggaggagcg gccgccgagc
      121 ccaggctcag gcaggccgag gagccgggcg tgagatcggg aacgtcgatg atcccgtcgg
      181 cattgcagtc gcgatagaga ttgacctgga cgcgcggaat gcccggctcc cagttctccg
      241 ccgtggcata gcgcggatcg tcctccgccc gcgtcgtcgc gtagcggacg atgcccgtga
      301 tgccgccgtt ctcgcccggc gcataggtcg acttgcccca ctcgatcacg ttggtctggc
      361 cgaggaagag ctgcatcccc tcgagcagca ccggaccggt ttcggtgcga tacggcaggc
      421 ccgcgttctc cgattgcggc tgcgggttca gccggtcgaa cgacggcatg gtccagccgt
      481 cgtggggcgg aacgggaccg ccggcgtcga cgacgatcgt ggcgcccgtg gccttgaagc
      541 gcgcgaagtc gacttcagcg atgagccagt tgaagaaggg gaacacctcg ttgaagacag
      601 ccaagccctg cgggttggtg ggataggact ggtagatgct gccgtcgcgg aagcgcaggt
      661 tgatgttctg gcccgggatc ggggcttcgc cggcgtcgcg gaaaccgttc tggttggtgt
      721 cgaagaagac gcggctttcg aagcggccgt accagttgaa gaccggcacc tcctggagat
      781 tcacggtgtt cacgccggcg ggcacggtca cgttgctcgt cgagatgatc atgtcgaggt
      841 tgtcgtccca gatcacgagc tggtactgcc ccggcgacac gttggggatg gagaacgtgc
      901 tgtcggcgtt gcaggccttg gtgaacacgc cgcgcccgcc gaccgcgggc atttcgttca
      961 ggcccaccca gcattgcgga aacggcgcgc cgttgaagaa cgcgtagtcc ggcggccgcg
     1021 aattgtggat gttgacgacc cgcccggtga tggtggtcgg gccgttcagc acattggccg
     1081 cgttcaacgt gcgcacgaag cccatgaaca cgtgatggcc cggcggcccg aattcctgga
     1141 agtacggagg ctcgttcgcc ttcacccacg cgtcgatgcc cttcgtgccc tcgagggtcg
     1201 aggtctggtg ccagtcgctg cccgccggcg gcgagacggt gatcgtgtac ttcgccgggt
     1261 agagattctt gatccgcgcc gtgccgtcgg ggccggagat gatgacgccg ttgcccagcg
     1321 cgagcaccgc gggggagccg tcgggattga acacgaagtc gccgttgggc agttgctggt
     1381 aggtcgtgcc cagcgggttg ccgaaggcgt ccttcgtcac ctggccgccg gattgcccgt
     1441 acgtgccgcc ggcttcgatg agcgtgaccg tgaagccgcc aagccccagc tcctgcggca
     1501 gattcggcgc gccgttgatc ggctggttgt cctcgaacac gaagaccgag atctgcgcgg
     1561 tggggatggg ctgcgtgttc acccagaccg aggccgaggt ctgtccgggg gcgatggggg
     1621 tgcctcccat ctggtaaccc gactgcggaa ggatcgaaag gtagtagcgc ttggtcggat
     1681 cgagagccgc gaccgggttg gcgccatttc ccgtcatcac cacgggcatg tagctcgtgt
     1741 ggaagttgag ggagaggttg cccgcgccgg cgggctgccc gggaaccgaa gccttcgtca
     1801 tgtcctcctg caccagccag cggtagttgg tcaccgcgag gccgtcggag ccgacgacgc
     1861 tgatgttgag cgcaagggcc tcgtaagcgc acagcagccc caacaacgca atggcgaata
     1921 ctcgccccgc aacgagcaag ccccagacga tccaacgggt aatgacattc atcgtgcgcc
     1981 tccctctcga caattcgccc cgttccggca aggggccgga cactcggggt tcttggatcg
     2041 aagtctgcgg acagtcgggg aggaaatcag gaaggcggac tgcgcaattc acccctggca
     2101 tccccctcca ttggcggtag ggaaggactg taggaggcgc tgcgtgtcct gcgggtgctg
     2161 cggcgcggtg cgccggcgag gcgaagcgcg ggtcaggagt tcgcgctgcc cagcaggccg
     2221 ttcgcgatgg cgaacgcggt gagcgcggcg atgctgtgga cgtcgagctt cttcatgaga
     2281 ttggagcgat gcttctcgac ggtcttcggg ctgaggctca ggaattccgc gatctccttg
     2341 ttgtggtggc cttccgccac cagcttcagg atctgccgct cgcgctgcgt cagcatgtcg
     2401 acgggccgga tcgtcagggg cgccctgagg gcgcccagat aggtcgagat gacgcggccc
     2461 gaaatctcgg ggctgaggaa ggtcttgcct cccaggacgg tgcgaatggc ggtgcgcaac
     2521 tcgtcgtgcg tcgcatcctt cagcacatag ccgttggcgc cggccctgag cgcctcgtgg
     2581 atgtattcgt ccgtcttgtg gatcgtcagc acgatgaccc gggtctccgg ctcgcggcgg
     2641 cgaatctccg cgatggcctc gatgccgttc atgcccggca tcgaaatgtc catcaggacc
     2701 aggtcgggct tcagcgtgct gcagacgcgg aacgcatccc gtccgttatc ggcttctccg
     2761 accacctcga ggtcggcgtc cttggccagg agcgcccgca ggccggtgcg gaagagcgtg
     2821 tggtcctcga cgatgacgat tcgccagtgc gtggccatgg tgatactcgc cggcacctta
     2881 cgagcccgcg gccgggtgca agcgccaggt ggcactcacg gtcactcccc ggcccggcgc
     2941 ggcctcgacg gcaaacgttc caccgcagct ttcggcgcgt tcacgcgagc agcaatggcc
     3001 gcatcgtctc tccaggccct tgcgcgcctc gaccgcgacc ggatcgaatc cgaccccgtt
     3061 gtcggcgatg gtcagccgaa gcagttccgc atcgcgcgcg agacggaccc gcacccgggt
     3121 ggcatgggaa tgcttcgcga cattgttcat cgcctcctgc acgatgcgga acaccggcag
     3181 ccggagcgct tcgtcgatgt cggtctccgc ggcgagcatt tcgacctcca ccgaaatgtc
     3241 ccggtagatc gactcgaact cgcgcacgaa ccaggtcagc gtggcgacga ggcccaggtc
     3301 gtccagggtg gatggccgca ggttcatcga aagacgccgc acctcctcca gcgccgcctt
     3361 caccgtggac accaggccgt cgatcgtctc gcgggcttgc cccgtttcgt tgtccccgat
     3421 cgcgccggac gccgtctgca gcccgaattt caccgcgctc aacatctgcc cgagaccgtc
     3481 gtgcaggtcg gccgcgatgc gcttgcgttc actttcctgc gcggcctgca tcgcgcacga
     3541 gaggcgtcgc gcgtcgtcgc acggcggctg caccggaacc ttcaccaggg ggggcgcctc
     3601 gcgcaacacc gcccaccata cgggacccac atcgtcttgc gcggcgatcc gctcgaaagt
     3661 cacgtcgacc ggcaggggcc ggccatcgcc ccgtacgagc gtcgctccgt atcgcgcgcg
     3721 atggttcggc gatagcgcgg caaagcgcgc gcgaatgtcg tcgcgctcgc ttcggtcgaa
     3781 aagccccagg agggggcgcc cgaccaggtc atccgtggag cagcccgaca gcgccgcgaa
     3841 cttcgcgctg gcgaagacga ttgccttctg gcggtccatg atcaggaggg cctcgtccat
     3901 caccgcggcg gctacacgaa gcctttgttc gctgcggtgc aacgcgagcg tcgtgcaccg
     3961 gagcacgcgg gaatcgtggt tcgccctcgc ggtgcccgcg acggccgcgg cgggcttgcc
     4021 ggtggcgttt tccgcgaatg cctccctcgc cgaaaaacga agcgatgctc gaaacaggct
     4081 tccgatcgtg cccatcgggt cctccccaat acctatgcgc tgtcccgccc gtgaagggtc
     4141 ttgtcgggtt attgaactgc ttatgactgc ctcgaacgag cccgccggat gcccctgccg
     4201 cagtgaatcg acaaggggtc gcagccggcg cgaattgccc ctgcccgcta ataccgccag
     4261 gacacccgca gcatcggatt gccggacgcg ttcctgacca ccgacagctt gccgcccgag
     4321 atgatcgcgc gctcgcgggc cgccatgaat gcgctttccg aatcgcgcgc cccgctctcg
     4381 tcgctcatcg ccgcgacaac ggcgtccgcg tcgtcctcga tgacgagcgt gagcatgcga
     4441 ttgctcgcct cgagcccgat gcgcatccct gtcgccccgg ggtggcctgc aacgatcttc
     4501 agtgcggttt cgacgatacg gtaaaccacg atcttcaggg aggcgggaat gtcggtttca
     4561 tgtgcggtaa attgtcgcgc cacccggaat tcgggagtcg cctcaaggaa atcggcgcac
     4621 aacgcctcga gtgtcgggac caggccgaag tcgtccaggc ccgacggccg aagatccatc
     4681 gcgatcgtgc gcacctggcc gatcgcgctc tgcaggccgg ggatgacgga ctcgagagag
     4741 ctggtcccgt cgggcccggt gtcgccacgg gcgttctcca ccgcgagctt cacggcgctc
     4801 agcgtctgcg cgaggccctc gtgcaggtcg gtggcgaact tcttccgctc gctctcctcg
     4861 cggcgcaggt tctcgcgcga gagctgttcc agcaaggcgt tcttctcccg gatggtcctt
     4921 tgctgggttt cgatcagttc gtgcgagcgg tacacgagca gcagcagcgc ggcatacagg
     4981 ccggtgagaa tcagcgccat cacggcgagc atctgcagtt cggaactctc cgcctcggcc
     5041 accagcgcgt ccaccgacgt gtacatctcg atgacgcccg cgaccggctc ggtaggccgc
     5101 ctgcgaacgg ggatataggt ctggacgaga ttgtcgtcct cggtgacctg gtcgaaggga
     5161 ttgaaggaat cccggtacac gagcctcgcc gacacctttc cgcccatcgc cgtacggaag
     5221 ccctggtttg cggactggtc gtttccgatc tggcgcgatt ccgtcgagaa cagaaccaat
     5281 ccgccccggt tgtagatctt gattcgcgca atctgcgtgt cgcgcatcat gtcggcgatg
     5341 gcgtcctgca gcgcttcggg catcgccacg gacagcgggt cgcgccccgc tgcgccagcg
     5401 acctcgaaat actcgaccag gtacggacgg atcggatgca gggcaagcct cgcgacggtc
     5461 acgccgctgc cctcggcgat cgtcgtgacg ctgcgaatca cgatcgcgcg atacatgacg
     5521 aatatggcca ccgccgcgat aaagatgccg gccaggctcg ccatcgagta attgcggaga
     5581 aaccccttca tcgcgctccc tgcgcctgcg gccattcgag gtcgcggccg acaaaaccgg
     5641 ccccgactat gccccatgcc gggaccggtc gcaacacgcg gccgggcgcg gcggctgcct
     5701 gacacgaccg tgcccacgcg cgtctttcca gagtgaaaaa cgcggccgga ggcctgaaag
     5761 ccgccaacgc gacctacagt gttcgcctac ccgcgattgc gggttttacg gtgcccgaat
     5821 acgcaacttg cccgcctgat ttcggccggc ccctccgcga gacttggccc agccctgctt
     5881 gaagcgggga tcgtgatggg tccattcaat agggggaggg atcatgagaa caggcatcag
     5941 gacattggct tgcgcaactc tgctagccct gggggcgatg ttcgcccgtg gggcgcttgc
     6001 cgagtcgccg aattcgccga tcatcgcaga ggcgcgcgcc gacgatcttg gcgtgcttca
     6061 catcatcggg gtgaacttcg tcgccacggg cggcctgccg cccgtcgtca ggctgggcac
     6121 gaaaagcctg ccgctcgtgg tgacccttgc cacgcccacg caaatcgacg cgctgctgcc
     6181 gccgggtctt gcccctggca gctatttgct gagcgtggag aaggggaaga aggcgtccaa
     6241 ggccagcgac gacgacggcc ggtcggacga attctgggtc acgctgggcg cggccgggcc
     6301 gaagggtgac aagggcgacc cggggccccc tggcccgggc ggagacggaa cgacgctacc
     6361 cggaccgata gggccgccag gccccccggg accgcaaggg ccgcaaggcg cggcgggcgt
     6421 gccgggcgcg cagggattgc ccggaccttc gggacccgca ggcccgcagg gattgaccgg
     6481 acccgccgga cccgcaggcc cgcagggatt gaccggaccc gccggacccg caggccccca
     6541 gggattgacc ggacccgcag gcccgcaggg attgaccgga ccgacagggc cgggtggtcc
     6601 gcaaggattg ccggggccgc aggggttgac cggtgccccg ggacttcccg gtccgcaggg
     6661 actgccgggg ccccagggcc tgaccggtcc gcaagggccg aagggggatc cgggacccgc
     6721 ggacacttcc atgcgaatcg acgtgtgcgc catcgtcctg aagctgaacg cgcaattcgc
     6781 ggcgggactt ccgctgcccg cgtattgcgc gaatccgtcc gccagcttca acgcgacggg
     6841 cactttccag acgatgacga tccccgtcac cggcaactac ctcatcgagg ccgccggcgc
     6901 tcagggcggc gcggattcgc tcacctacgc gcatgccggc gggctgggtg cccgcgtgag
     6961 cggcatcgtg ccgcttgctg cgggtgacgt gctttgcatc atcgtcggca tgcggcctgc
     7021 cggtcccgcc ggtccctacg atgccggcgg cggtggcggc ggctcgttcg tcttcaggag
     7081 cgacgccggc tgcactgcca aacccgcgct gcccttgata gtggcgggtg gcggtggcgg
     7141 cggggccggc agcggcggcg aggtcacggt gaatggcggc aatggcgcgg ggttgggcgg
     7201 cgtcaacgga gcgggcggct ccgcctatat ctccggactc tccgagtatc acagcggcgg
     7261 cggcggggcg ggctggtcga gtctcggcca gggaggttca ccggccacgt tcgccggcgg
     7321 cggcggccac tgggcgggcg gtgcgggcgc gagctacctc aacacgcttt ccagcccggg
     7381 cggcttcggc ggcggcggcg gtgccggcat ggtctgcgaa gccggttgcg gaagcggcgg
     7441 aggcggcggc ttcagcggcg gcggaggtgg tgcgcgcacg acggacttgc acgcgggtgg
     7501 cggcggctcg tacatcagcg gaaccagccc ggtctcgacg acggcggtac aggcagggca
     7561 cggttacgtg gcgataacgc tccagtaggg cggggaggcc cggatccggg cctagatttc
     7621 ctcgggcacc atgcggatcg ccccgcaggg acattccgtg gcgcagatgc cgcagccctt
     7681 gcagtagtcg tagttgaagc ggaagcgatt tcccggcccc agcttcacca cggcgttgtc
     7741 ggggcatacg ccgtagcagt tgtcgcactc gaagcagttg ccgcaggaaa ggcaacggcg
     7801 cgcctcgaag agcgcggtgc cctcgtccag cgccatcacc acctcgtcga aggaggtgac
     7861 gcgcttctgc atgtcgagta tcgggcgcac cgtcttcgag gcgtccgagt agtaccaggt
     7921 gttgagcttg ccgaacccgg cgagctcgtg cttcggcccc gggtcgtggg tgccgccgcg
     7981 aagccacgcg tcgatgtggc gcgcggcctt cttgccgtgg ccgatggcga ccgtcaccgt
     8041 gcgctcggcg ggcaccatgt cgccgcccgc gaagatgccg gggtgcgaag tcatcatgtt
     8101 ggggccgacc ttcaccacgc cgtcggcgat ctccatgccc ggcacgcggt cgagcagcga
     8161 gaggtccacg tcctgcccga gggccagcac gacggagtcg gcctcgagct tctccagttc
     8221 gcccgtgggc tgcggcacgc ccttcgcgtc gagctccatc ctctcgaccg tgatcgagcc
     8281 ttcgcccgcc tgcttgatcg tcgagagcca cttgatgagc acgccttcgt ccagcgcctc
     8341 ctgcacctcg aagtcgtggg ccggcatctt ctcgcgcgtg cggcggtaga cgatcatcgc
     8401 ctcctccgcg cccaggcgcc gcgcggtgcg cgcgacgtct atggccgtgt tgccgccacc
     8461 atagacgacc acgcgccggc cgagcagcgg cttttcctcg ccttccatgc cgcgcagcac
     8521 ctgcacggca tccatgatct gggccgcctc gcccgccggg atgtaggcgc gcttcgcgat
     8581 gtgcgccccc acggccagga acgcggcgtc gaagccgccc gccttcatcg aggcgaggac
     8641 gtcgtcgacc ttcgcgttga cctcgagctt cacgccgagg tcgaggatgc gctgcacctc
     8701 gccgtcgagc acgtcgcgcg gcaggcggta cttggggatg ccgaagcgca tcatgccgcc
     8761 ggccatgggg ccggcctcgt ggatcgtgac cgcgtggccg aggcgcgcga ggtggtaggc
     8821 cgcggagagc cccgaagggc cggcgccgac caccagcacg cgcttgcccg tggccggcgc
     8881 gggcgggtcg aatttccagc cgcgcttcag ggcctcgtcg cccaggaaat gctccacgga
     8941 gttgatgccc accgacgagt cgagctgcgc ccggttgcag gagctctcgc aggggtgata
     9001 gcacacgcgg cccatcacgc cgggcagcgg gttgttctgc acgagcgagc gccaggccgc
     9061 ctggtaatcg ccggactcgg cgtggaagag ccacgcctgg atgttctcgc ccgccgggca
     9121 ggcgttgttg cacggcggca ggcggtcgac atactcgggg cggctggtgc gccacgagcc
     9181 ggtgtggttg gccaggctgg agcccggatc cagcgtgatg gcgaagggtt ttctcatgct
     9241 tcctcgagca ggcggtactt gcggatgttg cggtccgcgt tttcctggat gtggcggatg
     9301 acgtcctcgc gcggcttggg cgagaagagg tgggcgtagc gcttctgcgg cttcaggtat
     9361 tcctcgacgg gcacctggcg ccggatcttc gcgaccccgg tgacctcgcc gttctccgcc
     9421 tcgaacaccg gaaagatgcc ggtttcgtgc gccaggcgcg ccatgcggat ggtgagcgac
     9481 gagtcgtgcc cccagcccag cgggcacggc acgaggatgt gcaggtagcg cgcgccgcgg
     9541 aactgcatcg cgcggcgcac cttcgcctcg aggtcgcgca gctccgacac cgtggcggtg
     9601 gcgacgtacg ggatgccgtg cgcgatggcg atggtgggaa gatccttgcc ctgcccgaag
     9661 gggttgccgg gggaaggccc gacggcggcc gtggtggccg ttcgcgcggc tcccggcgtg
     9721 gccgacgagc gctgcacgcc ggtattcatg taggcgccgt tgtcgtagca gatgtagagg
     9781 acgtcgtcgt ttcgctcgaa catgcccgac aggcacccga atccgatgtc ggtggtgccg
     9841 ccgtcgccgc cctgcgccac cacgcgcacg ttgtcgcggc ccttcacgcg catcgccgcg
     9901 gcgatgcccg tggccaccgc cgcggtgttg ccgaagaggg agtgaatcca cgggatctgc
     9961 cacgaggtct cggggtaggg cgtggagaac acctcgaggc agcccgtcgc attggccgcg
    10021 atgagctggc cccgcgtggc ctccatcgcc gcatcgatcg cgtagcgcgc gccgagggcc
    10081 tcgccgcagc cctggcaggc gcgatgcccg gagttgagcg agttgtggcg gcggacggtc
    10141 gactgcaccg aacgttcggc gtcgggaagc aggcggttgc ccaccgtgaa ggttcccgtc
    10201 tggtagaact tgaccggctg gaagctcatg gcggtgtccc tagcgcagct gcgcggtgac
    10261 gaggcccacg tcgcgcagca acgcctcggc cagggggccc gtgcgccgcg attcgcgctc
    10321 gcgggcgagc gcgcggtcca cgacggggcg gttgagatcg aggaacgtga gcgggggcag
    10381 gacgccttct tccgcttcga cgaaggcccg ggcgagggaa tcgcgcgtga tctgccggcc
    10441 gcccagcccc gcgatcaccg tatgcaccga ctcgggcggg ccgcggtgcg agacgcggat
    10501 gttggcggac acgatgccgc ccatgcccac cgcaaggcac ttctcgagca cgacgatgca
    10561 cttcgcgttg gcgagcgcgt cgcgcaccgc ggcgagcggc cacgggcgga agcaggtgat
    10621 gcccagcacc ccaatcctgt ggcccttctc gcgcagcgcg tccacggttt ccttgatggt
    10681 gcccagcacc gagcccagcg cgaccacgat ggtgtcggca tcctccgtgc ggtaggtctg
    10741 cgtgaggccg ccggaactgc gcccgaaggc gtcgcggaat tccgccgccc aggcggggat
    10801 gagctccagg gcctgcatct gcttggcgtg cgcgaggtag cgcacctcca tgaacgcctc
    10861 cggccccacc atcgcgccca tggaaacggg ccgctgcggg tcgagcacct ggcgcggatc
    10921 gaagggcggc aggaacgcat ccacctgctc ctgggtggga acgtccacgc gctcgtaggc
    10981 atgggtgagg atgaagccgt ccatgcagac catcaccggc agcgacagct cctcggcgag
    11041 gcggaaggcc tggatgtgca ggtcgagcgc ctcctggttc gtctcggcga agagctggat
    11101 ccagccgcag tcgcgctggc tcatcgaatc ggtgtggtcg ttccagatgt tgatgggcgc
    11161 gccgatggcg cggttggcca ccgtcatcac gatgggcagg cccattccgg aagcgttgaa
    11221 gacggcctcg gccatgaaga gcagcccctg gctcgcggtc gcggtgtagg cgcgcgcgcc
    11281 cgccgcggag gcgccgatgg ccacgctcat cgccgcgaac tcgctttcca cgttgatgaa
    11341 ttcgcacggc gcgagctcgc cggatttcac catctcgccc agctcctcga cgatgtgcgt
    11401 ctgcggggag atcgggtagg cgcagatcac ctcggggcgg cacagcgcca cgg
//
LOCUS       DASUEV010000357         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_668661, whole genome shotgun sequence.
ACCESSION   DASUEV010000357 DASUEV010000000
VERSION     DASUEV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668661"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VFV55_11305"
     CDS             complement(<1..177)
                     /locus_tag="VFV55_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX4944932.1"
                     /translation="MTTQSLQVIILAAGQGKRMHSRLPKVLHPLAGRPMLLHVLDSAR
                     RLAPTRVAVVIGHGG"
     gene            complement(406..828)
                     /locus_tag="VFV55_11310"
     CDS             complement(406..828)
                     /locus_tag="VFV55_11310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX4944933.1"
                     /translation="MAKTLHVDIVSAEQSIFSGEAEMIIAPGEAGELGVLPEHAALLT
                     RIKPGTVRIKIPGEDREEIIYVSGGMMEVQPHVVTVLADTSVRAENLDEAKALEAKRA
                     AEDAINNRTGLMEIAEAQAVLAESVAQLAAIRKLRQVR"
     gene            complement(835..2256)
                     /gene="atpD"
                     /locus_tag="VFV55_11315"
     CDS             complement(835..2256)
                     /gene="atpD"
                     /locus_tag="VFV55_11315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX4944934.1"
                     /translation="MAEGRIVQCIGAVIDIQFPREAMPNIYDALTLVEESDSFAEKGL
                     TFEVEQQLGDGIVRTIAMGSSDGLRRGMKVKGTGAPISVPVGPGTLGRVMDVLGRPID
                     ERGPVTTAERRSIHAAAPRFDELSPSVELLETGIKVVDLICPFAKGGKIGLFGGAGVG
                     KTVTMLELINNIATQHSGLSVFAGVGERTREGNDFYHEMSEAKVIVQEDLAQSKVAMV
                     FGQMNEPPGNRLRVALTGLTMAESFRDEGRDILLFIDNIYRFTLAGTEVSALLGRMPS
                     AVGYQPTLAEEMGRLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFGHLDATVVL
                     SRDIAALGIYPSVDPLDSTSRQVDPNVVGDEHYSTTRAVQAVLQRYKELRDIIAILGM
                     DELSPEDKLAVSRARKIQRFLSQPFHVAEVFTGSPGKYVTLKDTIKGFKMIVNGECDS
                     LPEQAFYMVGPIEEAFEKAKTLQ"
     gene            complement(2327..>3193)
                     /gene="atpG"
                     /locus_tag="VFV55_11320"
     CDS             complement(2327..>3193)
                     /gene="atpG"
                     /locus_tag="VFV55_11320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX4944935.1"
                     /translation="SREIRTKIKSVQNTRKITKAMEMVAASKMRKAQERMRHARPYGD
                     KIRNIAAHLANANPEYRHAFLVKREGVKGVGVILVTSDKGLCGGLNTNALRLLTGKVK
                     EWQEEGRKVQFTAFGNKGFSFLQRFGGQVVSHLTHLGDTPHLEKLVGPVRVMVDAYNS
                     GEVGAIFVIYTRFINTMKQEAVVEQLLPLTADRLLETDRTARGGWDYLYEPDAKTVLD
                     QLLGRYLEAIVYQALAENIASEQSARMVAMKSASDNASSVIDQLTLVYNKSRQAAITK
                     ELSEIVGGAAAV"
BASE COUNT          497 a         1032 c         1062 g          602 t
ORIGIN      
        1 gccgccgtgt ccgatcacga cagccaccct cgtgggtgca aggcgccgcg ccgaatcgag
       61 cacgtgcagc aacatcggcc ggccggccag cggatgcagc accttgggca ggcgcgaatg
      121 catgcgcttg ccctgtcctg cggcgaggat gattacctgg agggattggg tggtcatgcg
      181 ggatcggcga cgcggccggc gcgaaaaata aaaagggcag gatcgctgga tcctgcccaa
      241 atgtgttgcg cctctaaaac aaggagtttc gtgatctcct ccctcaaccc aggcaaccaa
      301 cccggttcag ggctcgtcgc attggtcctg cttccacgac gataagacag caataatttt
      361 accacaacgc aagcggcgat tgcaccacac cgcaccgcgc cgccactagc gtacctggcg
      421 cagcttcctg atggccgcga gctgggcgac cgattccgcc aggaccgcct gcgcctccgc
      481 gatttccatg aggcccgtgc ggttgttgat ggcatcctcg gccgcgcgct tggcttccag
      541 cgccttggcc tcgtcgaggt tctcggcacg cacggacgta tccgccagca ccgtcaccac
      601 gtgcggctgc acctccatca tgccgcccga cacatagatg atttcctcgc ggtcctcgcc
      661 cgggatcttg atgcgcaccg tgcccggctt gatccgcgta aggagcgcgg cgtgctccgg
      721 gagcaccccg agctcgcccg cctcgccggg ggcgatgatc atctcggctt ctcccgagaa
      781 gatcgactgc tcggcgctga cgatgtcgac gtggagggtc ttggccatgg gggcttattg
      841 cagggtcttg gctttttcga acgcttcctc gatcgggccg accatgtaga aggcctgctc
      901 gggaaggctg tcgcattcgc cgttcacgat catcttgaac cccttgatcg tgtccttgag
      961 cgtgacgtac ttccccggcg agccggtgaa gacctcggcc acgtggaacg gctgcgacag
     1021 gaagcgctgg atcttgcgcg cgcgggacac cgcgagcttg tcctcggggg aaagctcgtc
     1081 catgcccagg atcgcgatga tgtcgcgcag ttccttgtag cgctgcagca cggcctgcac
     1141 ggcgcgggtc gtggaatagt gttcgtcgcc gaccacgttc gggtcgacct ggcgggaggt
     1201 ggaatcgagc gggtccacgg agggatagat gccgagcgcc gcgatgtccc gcgaaagcac
     1261 cacggtcgcg tcgaggtggc cgaacgtcgt ggcgggtgaa gggtcggtca ggtcgtcggc
     1321 aggcacgtac acggcctgga tcgaggtgat cgagcccgtc ttcgtggacg tgatgcgctc
     1381 ctggaggcgc cccatttcct cggcaagggt cggctggtag cccacggccg acggcatgcg
     1441 gcccagcagg gccgacactt ccgtgcccgc aagcgtgaag cggtagatgt tgtcgatgaa
     1501 caggaggatg tcgcggccct cgtcgcggaa gctttccgcc atcgtcaggc cggtgagcgc
     1561 cacgcgcagg cggttgccgg gaggctcgtt catctgcccg aagaccatcg cgaccttcga
     1621 ctgcgcgagg tcttcctgca cgatgacctt cgcttccgac atctcgtggt agaagtcgtt
     1681 gccctcgcgc gtgcgctcgc ccacgcccgc gaacaccgac aggcccgagt gctgcgtggc
     1741 gatgttgttg atgagttcga gcatcgtcac ggtcttgccc acgccggcgc caccgaacag
     1801 gccgatcttg ccgcccttgg cgaacgggca gatgaggtcc accaccttga tccccgtctc
     1861 gaggagctcc acggatgggg agagttcgtc gaacctgggg gccgcagcat ggatcgagcg
     1921 gcgttcggcc gtcgtcaccg gcccgcgctc gtcgatcggg cggccgagca cgtccatcac
     1981 gcggccgagg gtaccggggc cgaccggaac cgagatcggc gcgccggtgc ccttgacctt
     2041 catcccgcgg cgcaggccgt cggaggaacc catcgcgatg gtgcggacga tgccatcgcc
     2101 gagctgctgc tcgacttcga acgtgaggcc cttctcggcg aacgagtcgg attcctcgac
     2161 gagggtgagc gcgtcgtaga tgttgggcat cgcctcgcgc gggaactgga tgtcgatcac
     2221 cgcgccgatg cactggacga tcctgccttc tgccatttgg gtcgcactca tgttgatgtc
     2281 ccgttgatgg ttcgtgtctc gaagccgcgg ctcgaacgga gcagccttac acggccgccg
     2341 cgccgccgac gatctccgaa agctccttcg tgatcgccgc ctggcgcgac ttgttgtaga
     2401 cgagggtgag ctggtcgatc acgctggagg cgttgtcgct cgcggacttc atcgcgacca
     2461 tccgcgccga ctgctcgctg gcgatattct ccgcgagggc ctggtagacg atagcctcga
     2521 ggtagcggcc gaggagctgg tcgaggaccg tcttcgcgtc cggctcgtag aggtagtccc
     2581 agccgccgcg cgcggtgcgg tcggtctcca gcaggcgatc cgcggtgagg ggcaggagct
     2641 gctcgacaac cgcctcctgc ttcatggtgt tgatgaagcg cgtgtagatc acgaagatcg
     2701 cgccgacctc gccgctgttg taggcgtcga ccatcacgcg cacgggcccc acgagcttct
     2761 cgaggtgggg cgtgtcgccc aggtgcgtga ggtgcgagac gacctgcccg ccgaatcgct
     2821 gcaggaagct gaatcccttg ttgccgaagg ccgtgaactg caccttgcgg ccttcttcct
     2881 gccactcctt caccttgccg gtgagcaggc gcagggcgtt ggtgttgagg ccgccgcaca
     2941 gccccttgtc ggacgtgacc agaatgacgc ccactccctt cacgccctcg cgcttcacga
     3001 ggaaggcgtg ccggtactcg gggttcgcgt tggcgaggtg cgcggcgatg ttcctgatct
     3061 tgtcgccgta cgggcgcgcg tggcgcatgc gctcctgggc cttcctcatc ttggaggccg
     3121 cgaccatctc catggccttg gtgatcttgc gcgtgttctg cacgctcttg atcttggtgc
     3181 ggatttctcg gga
//
LOCUS       DASUEV010000358         8052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_333465, whole genome shotgun sequence.
ACCESSION   DASUEV010000358 DASUEV010000000
VERSION     DASUEV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333465"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..566)
                     /locus_tag="VFV55_11325"
     CDS             complement(9..566)
                     /locus_tag="VFV55_11325"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX4944936.1"
                     /translation="MKTRKESTVLPLRRFASGIVAALALCLAAGAGAEPPQTLTPARD
                     LKRAPDITLETVGGGKVRLADLRGKVVVVNFWATWCPPCKREMPSLQRLSELLKGEPV
                     EVIAINAGEDDEDIERFRAGMDPPLTFRLALDRNGDAMQAFAVHGLPTTLVIDRKGRI
                     AFHALGDRNFEDPNIVATIRALAAK"
     gene            complement(526..1026)
                     /locus_tag="VFV55_11330"
     CDS             complement(526..1026)
                     /locus_tag="VFV55_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944937.1"
                     /translation="MDSPNAAGPPSREISRDGIVIKVTPLHAESVAGFLIGRGFPTRE
                     AKRYAEGCVVNVMLRNESGRETIDYDLRSWRTRSDGGTSRRLSTREEFLKQWRAAALP
                     TAALMGFEWSQLPTPLELHAGDSARGMVHTGLAAGSRFDLLLEWKAGGRTHREDAKGI
                     DCAPPS"
     gene            complement(1133..1591)
                     /locus_tag="VFV55_11335"
     CDS             complement(1133..1591)
                     /locus_tag="VFV55_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944938.1"
                     /translation="MSLSLLMGNANASDDKAALTGLKEVKVAYDIKEGDPKLLLNRLD
                     IIDETRQSLIQQGVTPHFILAFRGPATRLLQTDPEMIKPEFREQAAKVAQKVSQMSTA
                     AGVEGFEQCAVAVREQGTKAEKVLPQVRVVGNGFISLMAYQAKGYAYIAP"
     gene            complement(1723..5487)
                     /gene="putA"
                     /locus_tag="VFV55_11340"
     CDS             complement(1723..5487)
                     /gene="putA"
                     /locus_tag="VFV55_11340"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX4944939.1"
                     /translation="MTTLEVPSQSTAGVTQPAISRGLARHGSPLREAITAAYHQAEPQ
                     AVAAQVRAATLDDTALARIRSRAGALVERVRAERSGASGVDALMSEFDLSSEEGIALM
                     CLAEALLRIPDAHTADGLIRDKLSRGDWRSHVGGSESFFVNAACWGLVVSGTVARVEP
                     EPKGLDGALADILRRAGEPVIRAATKAAMGFLGSQFVLGETIGGALERARRKEARGYR
                     YSFDMLGEASMTMADAESYRVKYVEAIDAVGRSSAGRGIYAGPGLSVKLSALHPRYSR
                     SKHERAMTELLPRLKELMVRAKRHDIGLTIDAEEADRLEISLDILEALAFDPDLAGWD
                     GLGFVIQSVQKRAPFVVDWTLDLARRSGRRLMIRLVKGAYWDSEVKRAQVAGLADYPV
                     FTRKSHTDLCYLACAAKLLAEPRLAFPQFATHNAATLCQVIELAGEHRDFEFQLLHGM
                     GETLYDQVVGARDFGVPCRIYAPVGTHETLLAYLVRRLLENGANSSFVNQIVDPEVPI
                     ARLLEDPVTTAKPFGGSAHPRVPAPPALYADRRNSRGIDLTDEHALAALEAGLAGAAR
                     ESWSATPMLGEGVVAADAGRAVPVYNPADTQAVVGRVADATIDDVDRAVALARGAGAW
                     RATAAAERAHILEKAADLLEENAARLIHLAVREAGKTLPNAVGEVREAVDFCRYYALR
                     VREEKLAAPLGVVACISPWNFPLAIFIGQVAAALAAGNAVIAKPAEQTPLIAAEAVRL
                     LREAGVPAPALQLLPGPGETVGSRLVGDPRVDGVVFTGSTEVAQIIHRTLSLRGNIPL
                     VAETGGQNAMIVDSSALPEQVVSDVLSSAFDSAGQRCSALRVLFVQEEIADHVLAMLA
                     GAMRELAVGDPALLSTDIGPIIDAAAKAGLDAHIAQLEKSAKPVARTPMPEAPGGHFF
                     APVAFEIGSIRELQREVFGPVLHVVRFRGEELAQVVDDVNATGFGLTLGIHSRLDSTI
                     DLIVDRARAGNVYVNRNIVGAVVGVQPFGGEGLSGTGPKAGGPLYLRALVREATAETA
                     PGNFSPPAIDAAPVLDFDAAIALLAAAEPGLKSVDRDAVLATLARQAGEVVAAIAEDC
                     AKAIEEGRERELPGPTGERNTWRTHPRGLTVALGDEGNLALAWLGQAMAAIAAGNPVL
                     LAHVGNAAVAARVAAWVQEAGWPGIAAASASTTQWLDLPQLAAVMADGAAHAAVAVAA
                     VAARAGARIPVVQPGASRYRYPVWRLEVERTVSVNTVAAGGNAHLLAQMD"
     gene            complement(5542..6063)
                     /locus_tag="VFV55_11345"
     CDS             complement(5542..6063)
                     /locus_tag="VFV55_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opioid growth factor receptor-related protein"
                     /protein_id="HEX4944940.1"
                     /translation="MNDGRLVSFYGHTGPDHRGRMLRDIRASDISWLEGTHDFIQWLF
                     PIPEPSRVNALAPLLTPADIEAFAANPGMRNELLLSLEVMLRFYGLELSGTPGAPRVE
                     RGANYATRSAEWLERPHNFLRISRILRSLTLLGCAPEARAMLECLEGIYAENAQEIGS
                     DTLRYWRRAIEPR"
     gene            6368..7156
                     /locus_tag="VFV55_11350"
     CDS             6368..7156
                     /locus_tag="VFV55_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944941.1"
                     /translation="MKDDSKDRGGRMMTNDGDAAVAEAVVRDCAPTGVLRVGINLGNP
                     VIAQRNRGGGDPGGVGPALGREIARRLGLPLKFVTYETAGGMADAVKEGAWDVAFLAV
                     DPARATDIAFTAPYVHIEGTYLVRDDSPARKVQDLDRAGVRIAVGLKTAYDLFLTRQV
                     KQAQLVREPTSVAALEAFVARGLDAAAGVRQPLEGFAASHPGLRVLDESFMVIRQASG
                     VPKGRPQAAAWLAGFIEEAKRTGFVEKALKESGVGEVTIAPPAS"
     gene            complement(7169..7645)
                     /locus_tag="VFV55_11355"
     CDS             complement(7169..7645)
                     /locus_tag="VFV55_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944942.1"
                     /translation="MPVAPERRAPAVADLLLVLAELEGHGVRYALLGGAAMAVHGFPR
                     MTRDIDCLFPVDARNNARLMAALKELGTKLRFDFLPDRKSLDQGFSTAAEGEIALDIL
                     FVAASRRFEDYARHIEEREIDGVRFKVLDVDGMLMSKETDRAVDIPDRQRLRGLKG"
     gene            complement(7605..>8052)
                     /locus_tag="VFV55_11360"
     CDS             complement(7605..>8052)
                     /locus_tag="VFV55_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944943.1"
                     /translation="KQVSVLRLAKRELGLSYPRLATTLGVSERTMEKWALDPSSGDHR
                     GMPLMARKFLCHLLEEAKRRHLSAGDRDSAERIDAIAANADPARMRVSLQAFDALQRT
                     ANALAPMRARPDRPRFFRTLAEKNSWEDKEILENARRARAKSARRR"
BASE COUNT         1248 a         2722 c         2817 g         1265 t
ORIGIN      
        1 gctccgtact acttcgccgc aagcgccctg atcgtcgcaa cgatgttggg gtcctcgaag
       61 ttccggtctc ccagggcatg gaacgcgatc cggcccttgc ggtcgatgac gagcgtggtc
      121 ggaagcccgt ggaccgcaaa agcctgcatc gcgtccccgt tgcggtcgag cgccagcctg
      181 aacgtcagtg gcgggtccat gcccgcccgg aacctttcga tgtcctcgtc atcctcgccc
      241 gcgttgatgg cgatgacctc gacgggctcg cccttgagca attcgctcag gcgctgcagc
      301 gaaggcattt cgcgcttgca gggcgggcac caggtcgccc agaaattcac cacgacgacc
      361 ttgccgcgca ggtcggcgag ccgcactttc ccgccaccca ccgtttccag cgtgatgtcg
      421 ggggcgcgct tgaggtcgcg agccggagtc agggtctgcg gaggttcggc gccggcgcct
      481 gccgcaaggc aaagggccaa agccgccacg ataccggagg cgaatctacg aagggggagc
      541 acagtcgatt cctttcgcgt cttcacggtg ggtcctgccg cccgccttcc actcgagcag
      601 caggtcgaat cgcgagcccg cggccaaccc cgtatggacc attccccgcg ccgagtcgcc
      661 cgcatgaagc tccagtggcg tgggaagctg cgaccattcg aatcccatga gcgccgccgt
      721 cgggagcgcg gcggcgcgcc actgcttcag gaactcctcc ctcgtggaaa ggcggcggga
      781 agtgccgccg tcggaccgcg tgcgccacga gcgaaggtcg tagtcgatcg tttcccgccc
      841 ggactcgttg cgcagcatca cgttgacgac gcagccctcg gcgtagcgtt tcgcctcgcg
      901 ggtcgggaaa ccgcggccga tgagaaatcc cgccacggac tcggcgtgca gtggcgtgac
      961 cttgatcacg atgccgtcgc gcgagatctc gcgcgacggc gggccggccg cgttcggcga
     1021 atccacgggt gcctgcgcgg cggcttgtcc cccgaatatc gccgccgcca gctgcgcgag
     1081 aagggcagca actgccgggc gtgcgcccgt caggcaggtt ccgcgcgcgc cgttacggag
     1141 cgatatacgc gtaccccttc gcctggtaag ccatgagcga gatgaagcca ttgccgacga
     1201 cgcgaacctg cggcagcacc ttctcggcct tggttccctg ctcgcgcacc gccacggcgc
     1261 attgctcgaa gccctcgacg ccggccgcgg tgctcatctg gctcaccttc tgcgccacct
     1321 tggccgcctg ctcgcggaac tccggcttga tcatctcggg gtcggtctgc agcagccggg
     1381 ttgccgggcc gcggaacgcg aggatgaagt gcggcgtcac gccctgctgg atcagcgatt
     1441 gccgcgtctc gtcgatgatg tcgaggcggt tgagcaggag cttgggatcg ccttccttga
     1501 tgtcgtaggc caccttcacc tccttcaggc cggtgagcgc agccttgtcg tcggaggcgt
     1561 tcgcgttgcc catcagaagc gaaagcgaca tggccgcgaa ccacattgaa ctgtatcgaa
     1621 catcgcgcat aggtccccca ggagacagtt attcggttgt cgggtcgaat gcccgttcgc
     1681 ttggatagca caaaatgcac cgccggcaaa ggtttgccgc gctcagtcca tctgcgccag
     1741 caggtgcgca ttgccgcccg cggccaccgt gttgaccgac acggtccgct ccacctcgag
     1801 gcgccaaacc ggatagcgat accgcgacgc gccgggttgg acgacgggaa ttcgtgcccc
     1861 ggcccgagcc gcaaccgcgg ccacggcgac cgcggcatgc gcggcaccgt cggccatgac
     1921 cgcggcaagc tgcggaagat cgagccattg cgtggtggac gcggacgcgg cggcgatgcc
     1981 gggccagccg gcctcctgca cccacgcggc gacccgcgcc gccactgccg cattgcccac
     2041 gtgggcgaga agcacggggt tgcctgcggc gatggccgcc atcgcctggc cgagccaggc
     2101 aagggcgaga ttgccttcat cgcccagtgc gaccgtgagc ccgcgcggat gcgtccgcca
     2161 cgtattgcgc tcgcccgtgg gcccgggcag ctcgcgttcc cgcccttcct cgatcgcctt
     2221 cgcgcagtct tcggcgatgg ccgccacgac ttcgcctgcc tgcctggcga gcgtggccag
     2281 caccgcgtcg cgatcgacgg acttcagtcc cggctcggcc gccgccagca aggcgatggc
     2341 ggcatcgaaa tccaggaccg gtgcggcgtc tatcgcgggt ggcgagaaat ttcccggcgc
     2401 ggtttcggcc gtggcttcgc gcaccagcgc gcgcaggtac agcgggccgc cggccttggg
     2461 tcccgtgccg gacaggcctt ccccgccgaa aggctgcacg ccgaccacgg cgcccacgat
     2521 gttgcggttc acatagacat tgccggcacg ggcccgatcc acgatgaggt cgatcgtgga
     2581 atcgaggcgg ctgtggatgc cgagcgtgag tccgaacccc gtggcgttca cgtcgtccac
     2641 gacctgcgcc agttcctcgc cgcggaagcg caccacgtgc aggaccgggc cgaacacctc
     2701 gcgctgcagc tcgcggatcg agccgatctc gaacgcgacg ggcgcgaaga agtggccgcc
     2761 gggcgcttcc ggcatcggcg tgcgggcaac gggcttcgcc gatttctcga gttgcgcgat
     2821 gtgggcgtcg agaccagcct tcgcggctgc gtcgatgatc ggcccgatgt cggtcgacag
     2881 gagcgcgggg tctcccacgg ccagttcgcg catcgcgccc gcaagcatcg ccaggacgtg
     2941 gtcggcaatc tcctcctgca cgaagagcac gcgcagcgcc gagcaccgtt ggccggcgct
     3001 gtcgaaggcc gacgagagga cgtcggagac gacctgctcg ggcagcgccg aggaatcgac
     3061 gatcatcgcg ttctgccccc cggtctcggc gacgaggggg atgttgccgc gcagggagag
     3121 cgtgcgatgg atgatctgcg cgacttcggt ggagccggtg aacacgacgc cgtccacgcg
     3181 agggtcgccg acgagacgcg agccgaccgt ctctccgggt cccggaagga gctgcagggc
     3241 aggggcgggc acgccggctt cgcgcaggag gcgcaccgcc tctgcggcga tgagcggcgt
     3301 ctgctcggcg ggcttggcga tgacggcgtt gccggcggcg agcgccgcgg ccacctgccc
     3361 gatgaaaatc gccagcggga agttccacgg gctgatgcac gcgaccacgc cgagaggcgc
     3421 ggcgagcttt tcctcgcgca cgcgcagcgc gtagtagcgg cagaagtcga ccgcctcgcg
     3481 gacttcgccc acggcattgg gaagcgtctt gcccgcttcg cgcaccgcga ggtggatgag
     3541 gcgcgcggcg ttttcctcga ggagatcggc ggccttttcg agaatgtgcg cacgctccgc
     3601 cgccgccgta gcccgccacg cgcccgcgcc gcgcgcaagg gcaacagcgc gatccacgtc
     3661 gtcgatcgtg gcgtcggcaa cgcggcccac gaccgcctgc gtatcggcgg ggttgtacac
     3721 cggcacggca cgaccggcat ccgcggcaac gacaccctcg cccagcatcg gggtggcaga
     3781 ccacgattcg cgcgccgcgc ccgcgagacc ggcttcgagc gcggcgagcg cgtgctcgtc
     3841 ggtgaggtcg atgccgcgcg agttgcggcg atcggcgtag agagcgggtg gtgccggcac
     3901 gcgcggatgc gcgcttccgc cgaacggctt cgcggtcgtg accgggtcct ccagcaggcg
     3961 cgcgatcggc acctcgggat cgacgatctg gttcacgaag gacgaattgg cgccgttttc
     4021 gagcagccgc cgcacgaggt aggcgagcag cgtctcgtgg gttcccacgg gggcgtagat
     4081 gcggcaaggc accccgaagt cccttgcgcc gaccacctgg tcgtagagcg tctcgcccat
     4141 gccgtgcagc agctggaact cgaagtcgcg gtgctcgccg gccagctcga tcacctggca
     4201 cagcgtcgcg gcattgtgcg tggcgaattg cgggaatgcg aggcggggct cggcaaggag
     4261 cttcgcggcg caggcgaggt agcagaggtc cgtgtgcgac ttgcgcgtga acaccggata
     4321 gtcggccagg ccggcgacct gcgcgcgctt cacttcggaa tcccagtagg cccccttgac
     4381 cagccggatc atgaggcggc ggccgctgcg ccgcgccagg tccagcgtcc agtcgacgac
     4441 gaagggcgcg cgcttctgca ccgactggat cacgaagccg agcccgtccc acccggcgag
     4501 atcggggtcg aacgccagcg cctcgagaat gtcgagcgaa atctccagcc ggtccgcctc
     4561 ctcggcatcg atggtgaggc cgatgtcgtg gcgtttcgcc cgtaccatca gttccttcag
     4621 ccgcggcagc agctccgtca tcgccctctc gtgcttggat cgcgaatagc gcgggtgcag
     4681 cgccgagagc ttcaccgaca ggcccggccc ggcgtagatg ccgcgccccg cggacgagcg
     4741 gcccacggca tcaatggcct cgacatattt cacgcgataa ctctcggcat cggccatcgt
     4801 catcgacgct tccccgagca tgtcgaacga gtaacggtag ccgcgcgctt ccttcctgcg
     4861 cgcgcgctcc agggcgccgc cgatcgtctc gccgagcacg aactgggagc ccaggaaccc
     4921 catggcagcc ttcgtggccg cgcgtatcac gggctcgccc gcgcgccgga ggatgtccgc
     4981 gagtgcgcca tcgaggccct tgggttcggg ctcgacccgc gcgaccgttc ccgaaacgac
     5041 caggccccag caggcggcgt tgacgaaaaa ggactcgctg ccgcccacgt gcgagcgcca
     5101 gtcgccccgc gagagcttgt cgcggatgag gccgtcggcg gtgtgcgcgt cggggatcct
     5161 gagcagcgct tccgccaggc acatgagcgc gatgccttcc tccgacgaca ggtcgaattc
     5221 gctcatgagc gcgtccacgc ccgaggcgcc ggaacgctcc gcccggacac gctcgacaag
     5281 ggcgccggca cgcgagcgga tgcgcgcgag cgcggtatcg tcgagggtcg cggcgcgaac
     5341 ctgcgcggcc acggcctgcg gctccgcctg gtggtaggcg gcggtaatgg cttcccgaag
     5401 cggcgagccg tgccgcgcga gaccccggga aatcgcaggc tgcgtaacgc ctgccgtgga
     5461 ttgcgaaggc acttcgagcg tcgtcatggc gcgaaaaggg tagcacgggc cccgcatggc
     5521 gggcgtgccg ttcgttgggg gctagcgcgg ctcgatcgcg cggcgccagt agcgcagggt
     5581 gtcggagccg atttcctgcg cgttctccgc gtagatgcct tcgaggcact cgagcattgc
     5641 ccgtgcctcg ggggcgcagc ccagcagggt cagcgagcgc aggatccggc tgatgcgcag
     5701 gaagttgtgc ggtctctcca gccattccgc gctgcgcgtg gcgtagttcg cacccctctc
     5761 gacccggggc gcgcccggcg ttccggaaag ctcgagcccg tagaagcgca gcatcacctc
     5821 cagcgacagc aggagctcgt tgcgcattcc ggggttcgcc gcgaacgcct cgatatccgc
     5881 cggcgtgagc agcggcgcca gggcatttac cctgctcggt tctggtatcg ggaacagcca
     5941 ctggatgaag tcgtgcgtgc cttccagcca ggagatgtcg gatgcgcgga tgtcgcgcag
     6001 catgcggccg cggtggtcgg gcccggtgtg gccgtagaac gagaccagcc tgccatcgtt
     6061 catcgctacc tccctcgtca actcggaatc ggtgccggga ccgttatacc gtgatccgtc
     6121 cggcgcgtct ttcggcattg cgcgaatgtt tgcgctatgt tccctccgac accggcgagc
     6181 gcgaccgagt tctccaccgc gacgtcggtc acgccatgac cggaaccgga gccgtggcga
     6241 tcgccgtggc cgcggcgatt tcaggtactg tcgcgcagtg cgccattcga gccggagccg
     6301 aaaggatggg tacgggtgcc aacaaattga accgatcgtg attgccgcga aacaggagaa
     6361 cgaaaccgtg aaagacgata gcaaggatcg aggcggccgc atgatgacga acgacggcga
     6421 tgcggcggtc gcggaagcgg tggtgcgcga ctgcgcgccc acgggcgtgc tgcgcgtggg
     6481 catcaatctc ggcaatccgg tgatcgcgca acgcaaccgc ggtggcgggg atcccggggg
     6541 tgtcggcccc gcgctggggc gcgagatcgc caggcgcctc ggcttgccgc tgaagttcgt
     6601 gacctacgag acggccggcg gcatggccga tgcggtgaag gagggcgctt gggacgtggc
     6661 cttcctcgcc gtcgatcccg cgcgcgcaac ggacatcgcg ttcacggcgc cctacgtcca
     6721 catcgagggt acctatctcg tgcgcgacga ttcgccggca cgcaaggtgc aggacctgga
     6781 ccgcgcgggc gtgcgcatcg ccgtggggct gaagaccgcc tacgacctgt tcctcacgcg
     6841 ccaggtcaag caggcgcagc tcgtgcgcga gccgacttcc gttgcggcgc tcgaggcgtt
     6901 cgtggcacgc gggctggatg cggcggcggg cgtccggcag ccgctggaag gcttcgcggc
     6961 gagtcacccg gggctgcgcg tcctcgacga gagcttcatg gtgatccgcc aggcgtcggg
     7021 ggttccgaag ggtaggccgc aggccgcagc ctggctcgcc ggcttcatcg aggaggcgaa
     7081 gcggaccggg ttcgtggaga aggcgttgaa ggaaagcggc gtgggcgaag tgacgattgc
     7141 tccgccagcc tcctgaggga gcagacgctc accccttgag gccgcgcaac cgctgccggt
     7201 ccgggatatc cacggcgcga tcggtttcct tcgacatgag cattccgtcg acgtcgagca
     7261 ccttgaaccg tacgccgtct atctcgcgct cttcgatgtg gcgcgcatag tcctcgaagc
     7321 ggcgcgaggc ggcgacgaac aggatgtcga gcgcgatctc gccctcggcc gccgtggaga
     7381 agccctggtc gagggacttc ctgtcgggaa ggaagtcgaa gcgaagtttc gtgccgagtt
     7441 ccttcagcgc agccatcagc cttgcgttgt tcctcgcgtc cacggggaag aggcagtcga
     7501 tgtcgcgggt catgcgcggg aacccgtgca cggccatggc cgccccgccg agcagggcgt
     7561 agcggactcc gtggccctcg agctcggcga gcacgagcag gaggtcagcg acggcgggcg
     7621 ctcttcgctc tggcgcgacg ggcattttcc agtatctcct tgtcctccca cgaattcttt
     7681 tcggcgagtg tcctgaagaa cctggggcgg tccggtctcg cacgcatcgg ggcaagtgcg
     7741 ttcgcggttc gctgcaacgc gtcgaaggct tgcagggaca cgcgcatccg cgccgggtcc
     7801 gcgttggccg cgatggcgtc gatgcgctcg gcggagtcgc gatcgcccgc cgaaaggtgg
     7861 cggcgtttcg cctcctcgag caggtgacac aggaacttgc gcgccatgag cggcatgccg
     7921 cgatggtcgc cggaggacgg gtcgagggcc catttttcca tcgttcgctc gctcacgccc
     7981 agggttgtcg cgagccgcgg gtacgatagt cccagttcgc gcttggcgag gcgcaggacg
     8041 gatacctgtt tc
//
LOCUS       DASUEV010000359        12512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_458124, whole genome shotgun sequence.
ACCESSION   DASUEV010000359 DASUEV010000000
VERSION     DASUEV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458124"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFV55_11365"
     CDS             complement(<1..456)
                     /locus_tag="VFV55_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944944.1"
                     /translation="MQVRKEVVADWVRVGRNASSEVLLADPRIALNQGLIVDRNGPVY
                     TEGETGSMASTTRKAVRSVRLSPGSSVDVGPYRLTAIPTPAGYSGAITVELVRPPEST
                     APEFIARAQRLTLASLRLPKRATALALFAFVAIAFFLLPAGRILDLPWKQ"
     gene            complement(524..1357)
                     /locus_tag="VFV55_11370"
     CDS             complement(524..1357)
                     /locus_tag="VFV55_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944945.1"
                     /translation="MRHQSILEYAHFRWFKAAIWLSALAGGVYLWHDPVLKPYGGTWL
                     GYALGTLGALLILWLLWFGVRKRRYASRMGTAQGWLSAHVYLGTALLVVATLHTGFEL
                     GWNVHTLAYVLMVATVASGFYGVYVYFTVPRLMTANLGEETLDAMLLKVADLDREIRE
                     KALSLPDPILRVVHASLDNTRLGGSLARIVTGRDRACPTAAAVRELPEIGKRLSGEAA
                     KLNHEVYTLLLQKNELLARARREVRFKARLDLWLYFHVPLAIALLGALSAHVISVFLY
                     W"
     gene            1527..2843
                     /gene="ccmI"
                     /locus_tag="VFV55_11375"
     CDS             1527..2843
                     /gene="ccmI"
                     /locus_tag="VFV55_11375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX4944946.1"
                     /translation="MLTFWIVAALLIVAALAFVLVPLLRPRARSGPTVREANLAVLRG
                     QRQDIETDVALGLLPRDARESALAELSARAEEDLAAPGESLQGPPSRPWVLAAFFAVL
                     VPAVTVGFYLWVGTPAATDPKAMTATAADAQPGQHQIEEMVVALEQKMRDRPDDVQGW
                     MLLARSFAATGKTEKALAAYAHLAQIAPQDASVLADYADALGLSQGRSLAGQPTELAM
                     RALQLDPRHPKALALAATAKLNGGDFAASLGYWERLYAVLPPGSEDATEVRNIIEDVR
                     SRAAAAGKPQPPSKILAAAPAPAAPVNPAMSAAPVPPMGAAPAKAAAGGKSVSGTVKL
                     ADSLAGKAAPADTLFIYARAESGSRMPLAIIRGGAGELPKAFVLDDSMGMSPAVKLST
                     TPSVVIEARVSKSGGAVTQPGDLIGVSKPVAPGAKDVAIVIDRLVP"
     gene            2883..3530
                     /gene="ccmA"
                     /locus_tag="VFV55_11380"
     CDS             2883..3530
                     /gene="ccmA"
                     /locus_tag="VFV55_11380"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX4944947.1"
                     /translation="MTGKHRSGAADILQVRELACRRGPALLFSGLGFEVASGELLCVR
                     GPNGCGKTTLLRCVTGLTRPDEGTILWHGESSRTEPERMRAGTAYAGHLPGLKDDLTA
                     EENLEFSLRLRGTASTAEQRREALAAVGLEKRRRLPARRLSAGQRRRIGLAWLSLDPA
                     EFWALDEPLTALDDAGQALFVGLLERHLANGGLALVATHHRLAPSSGVVRELRLA"
     gene            3544..4254
                     /gene="ccmB"
                     /locus_tag="VFV55_11385"
     CDS             3544..4254
                     /gene="ccmB"
                     /locus_tag="VFV55_11385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX4944948.1"
                     /translation="MTANLGALAPAQGSFSGGFAWAMARDLRLAWRHPDEAVLTVAFF
                     ALVASLFPLGIGAEPQLLRRIGPGVIWVGALLAAFLALPGLFAADYTDGSLEQMTLSP
                     HPALGWSAGKIAAHWIVSGVPLTLLSPLIGLQYGLAPDTLAVQALSLAIGTPVLSLLG
                     GACAALALGARGGGMLLALLALPLFVPVLIFGAGAAEAQATGLSPAPHLSLLAAALIA
                     ALVGLPLAIGAAVRIAVD"
     gene            4346..5071
                     /gene="ccmC"
                     /locus_tag="VFV55_11390"
     CDS             4346..5071
                     /gene="ccmC"
                     /locus_tag="VFV55_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX4944949.1"
                     /translation="MNWFYFSSPPTFYRLAGRLAPWFFIMAALLAAWGLYLGLLVAPT
                     DAQQGAVYRVIFIHVPTAWMSMFLYFVMACYCGLTLVFNTRLSAMMARAIAPTGAMLT
                     FIALWTGSFWGRPTWGTYWVWDARLTSELVLLFLYFGYMALANAFEDPRRGDRAAALL
                     ALIGVVNLPIIYFSVQWWNTLHQGSSVSFKGTSMHVTMLTGMLVLSLAAWMYAIGAAL
                     VRVRTVILERERRTQWVRELGAA"
     gene            5068..5250
                     /gene="ccmD"
                     /locus_tag="VFV55_11395"
     CDS             5068..5250
                     /gene="ccmD"
                     /locus_tag="VFV55_11395"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX4944950.1"
                     /translation="MTIYWSSWSEFWAMGGHAFFVWMSYGALLAAIIVEIVLLRVRRK
                     RALAAIEEERDIEAEN"
     gene            5234..5704
                     /gene="ccmE"
                     /locus_tag="VFV55_11400"
     CDS             5234..5704
                     /gene="ccmE"
                     /locus_tag="VFV55_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX4944951.1"
                     /translation="MKPRTKRALAIAGGLAALGVAAALVLNAFQSNLVFFFTPTQVAR
                     KEVPLDRTFRIGGLVEGGSVVRRKDALSVTFKVTDGAETIPVAYVGILPDLFKEGKGV
                     VAEGRVGKDGIFNASQVLAKHDENYMPPEAAEALKQAGHPMAKENFAGQGGIKR"
     gene            5701..7683
                     /locus_tag="VFV55_11405"
     CDS             5701..7683
                     /locus_tag="VFV55_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX4944952.1"
                     /translation="MIPEIGQLALVLALCLAVAQAFLPLAGAALGKHSWMVTSRPIAL
                     GQFLFVLLAWTALATSFVNNDFSVANVAEHSNLSLPAHYRFAATWGSHEGSMLLWALM
                     LGGWTAAVALRSRHLPERIAARVIGIMGLISTGFLLFILTTSNPFDRLLPPAANGRDL
                     NPLLQDPGMVMHPPILYMGYVGFAVAFAFSIAALLEGRLDSTWARWSRPWTTAAWCFL
                     SVGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGAFKSWTV
                     LLAILAFSLSLLGTFLVRSGVLTSVHAFATDPRRGVFILTFLAIVVGGSLALFAWRAP
                     RVGMGGQFAGVSRESMLLANNVLLGIAMTAVLLGTLYPLVLDTLGLGKISVGPPYFDT
                     VFFPLMAPAVFLMAIGPMASWKKAELPDLATRLKWALGVALACAVIVPFARGKWSPLV
                     VLGLFLAFWVVAATAVQFGTRIKASPQATLMGKLRANSAAWYGMQVAHLGVAVFIIGV
                     TMVKGFETERDIRMAPGETATIAGYEFKFTATREISGPNYGAVRGTFEVRREGSPDVI
                     RRMYPEKRMYQASGQTMTEAAIAAGLTGDLYVSLGEQVEGNAWGVRIYHKPFVDWIWG
                     GCLLMAFGGFLAIADRRYRLAKRTATVPGATLATGD"
     gene            7685..8218
                     /locus_tag="VFV55_11410"
     CDS             7685..8218
                     /locus_tag="VFV55_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX4944953.1"
                     /translation="MAKALKYLVPLAIFLVLCAFLAVGLTKDPREVPSPFIGKPAPAF
                     RLAKLDEPPASFSPEDMKGKVWLLNVWASWCVSCRVEHPVLNDMKRRGLVPIVGLNYK
                     DARTDASKWLQQHGDPYQLSVWDFEGKVGIDYGVYGAPETFVIDKQGIIRYKQIGPIT
                     PEALEKTILPLLAKLNA"
     gene            8215..8703
                     /locus_tag="VFV55_11415"
     CDS             8215..8703
                     /locus_tag="VFV55_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX4944954.1"
                     /translation="MKRFLVLLFLAASFAAWGQSSEIAHADPKVEARLKQISEELRCL
                     VCQNQTIADSNAPLALDLRQQIRGMVGAGKTDDEIRAYMVERYGDFVLYKPPFNAVTA
                     LLWIGPGALVVFGFAALYLMIRRRREAAGAAPQPDPARRREIDALLVSDDEPKPKPNA
                     KR"
     gene            complement(8711..9208)
                     /locus_tag="VFV55_11420"
     CDS             complement(8711..9208)
                     /locus_tag="VFV55_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4944955.1"
                     /translation="MDLRRFWRHIAMTPWKAARAFPPSARGAIGRAVAEHEKQHRGEI
                     RFVVEAELTSAQLWANLGARARAVELFAMLHVWDTAENTGVLVYVLLADRKVEIVADR
                     GISARVAQAEWQAICASMQEAFRAGRFAEGAVEGVTAISALLERHFPAEAANANELPD
                     QPVML"
     gene            complement(9208..10122)
                     /locus_tag="VFV55_11425"
     CDS             complement(9208..10122)
                     /locus_tag="VFV55_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX4944956.1"
                     /translation="MTRIAGRLLAGAAASFFVAFAALAADVTEPIPVPKLTGRVIDLT
                     ATLAAPESEAIAARLKAFEAAKGSQVAVLMVPAIGTETIEEFATRVTDEWKLGRAGVD
                     DGVLFVIAKQERRMRIQTGRGVQGVLTDALSRRIIAERVSPRFRAGDFAGGISDGVDA
                     IMKAIEGESLPPPAQAPSRGKVDSFSSIENFFWLAFVVVPVVGMILRSLVGRFLGAGL
                     TSGVTGLAAWVIFGSLLLGGIAAVITFLAVLFMGTSALGARRGGIGGGPWGGGGWSGG
                     GGGGFGGGGGGFSGGGGGFDGGGASGNW"
     gene            complement(10119..10766)
                     /locus_tag="VFV55_11430"
     CDS             complement(10119..10766)
                     /locus_tag="VFV55_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX4944957.1"
                     /translation="MRKFLTAFALAGTLALSGCGYNDFQTKDEEVKAGWAEVLNQYKR
                     RADLIPNLVSVVQGFAAQERDVLLGVTNARAKVGSIQMTPEALNDPNAMKNFQAAQGE
                     LSSALSRLMVVVENYPQLKSDANFRDLQSQLEGTENRITTARNRYIKTVQGYNILARQ
                     FPTNLTAMLFGYKEKASFTVENEKQISEPPKVDFSAKPAAAPAQPAAPAQPAPSK"
     gene            complement(10852..11613)
                     /locus_tag="VFV55_11435"
     CDS             complement(10852..11613)
                     /locus_tag="VFV55_11435"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX4944958.1"
                     /translation="MDDRILRELELLPVARLYGLASPAVRAAASEAPTVETPPAARPG
                     NGPARAALRAPAPAASPPVAGIEAMDWAALEETVRSCRRCVLCEQRKQAVFGVGNVAA
                     PWLFVGEGPGADEDEKGEPFVGQAGKLLDSMLAAAKLARGREVYIANVVKCRPPGNRV
                     PAPEEAAACAPFLDRQIDLIRPKLIVAMGKTAMVRLTGNESSLASQRGRVLDYRGIPL
                     VITYHPAYLLRNLPDKAKAWEDLLFARRTFAAAQA"
     gene            complement(11617..12078)
                     /gene="rimI"
                     /locus_tag="VFV55_11440"
     CDS             complement(11617..12078)
                     /gene="rimI"
                     /locus_tag="VFV55_11440"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX4944959.1"
                     /translation="MSALPRVQLNYRRMCPADLGVVADLEKSLYAFPWSLGNFRDSVN
                     AGYDCWVACSGEHVIGYAILMVALDEAHLLNFAVATGWQNQGIGRAFLDHMLGTARES
                     KCQIVYLEVRPSNLAARHLYREMGFQQIAIRPSYYPARGGREDALFLGITL"
     gene            complement(12075..>12512)
                     /gene="tsaB"
                     /locus_tag="VFV55_11445"
     CDS             complement(12075..>12512)
                     /gene="tsaB"
                     /locus_tag="VFV55_11445"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX4944960.1"
                     /translation="ALGASRPVVPVPSLVALAEQANASRVLVGLDARMGETYLAAYSR
                     MGGDWEAAIEPGLHSQGSLPALPGRDWVATGSGFDAFGWLRSAYAPQVGHRIENDLPR
                     AGSVARVAARKLARGEALAAEEAAPLYLRDKVALTTRERQAAK"
BASE COUNT         1860 a         4380 c         4208 g         2064 t
ORIGIN      
        1 ttgcttccac ggcaggtcga ggatgcgtcc ggccggcagg aggaagaagg cgatcgccac
       61 gaaggcgaag agcgccaggg ccgtcgcgcg tttcggaagg cgaagcgacg cgagcgtgag
      121 ccgttgcgcg cgcgcgatga attccggcgc tgtgctctcg ggcggccgca cgagttccac
      181 cgtgattgcc cccgaatacc cggcgggcgt ggggatcgcg gtgagcctgt aggggccgac
      241 gtcgaccgag gaacctggcg agaggcgaac ggagcgcacg gccttgcgcg tcgtgctcgc
      301 catcgatccc gtctcgccct ccgtgtagac gggaccattg cggtcgacga tgaggccctg
      361 gttgagcgcg atgcgcgggt ccgcgagcag gacctcgctc gaggcgttgc gtcccaccct
      421 cacccagtcg gccacgacct ccttgcgtac ctgcacggtg cggccggcgc ggttgcggct
      481 ctgcgagatg acgaggagtt tcaggtcggt ttcccgggag cggctaccag tagaggaaca
      541 ccgagatcac gtgcgccgac agtgcgccga gcagcgcgat cgcgaggggg acgtggaagt
      601 acagccagag gtcgagccgc gccttgaagc gcacttcgcg gcgcgcacgc gcgagcagct
      661 cgttcttctg caggagcagg gtgtagacct cgtggttcag cttcgcagcc tcgcccgaga
      721 ggcgcttgcc gatctcgggc aactcacgca ccgccgcggc cgtcgggcac gcgcgatcgc
      781 gccccgtgac gatgcgcgcc agggagccac cgagccgcgt gttgtcgagc gaggcgtgca
      841 ccacccggag gatcgggtcg ggaagcgaga gggccttctc gcggatttcg cggtcgagat
      901 cggcgacctt gagcagcatc gcgtcgagcg tctcctcgcc gagattggcc gtcatcaagc
      961 ggggcacggt gaaatagaca tacacgccat agaacccgct cgcgaccgtc gcgaccatca
     1021 gcacgtaggc aagcgtgtgc acgttccagc ccagttcgaa tccggtgtgc agcgtggcca
     1081 cgacgagcag cgccgtgccg agatagacat gggccgagag ccacccctgc gccgtgccca
     1141 tgcgcgatgc gtagcggcgc ttgcggacgc cgaaccacag cagccacagg atgagcagcg
     1201 ccccgagggt gcccagcgcg tagcccagcc aggtgccgcc atagggcttg aggacggggt
     1261 cgtgccacag gtagacaccg ccggccagcg cggaaagcca gattgccgcc ttgaaccaac
     1321 ggaaatgcgc gtactccagg atcgactggt gtcgcacccg atgctctccc ttccccgcca
     1381 gcttgttgat tcttatggag cgccagccag cagaggaagc atggcgcgat cgcattgtag
     1441 cggcccgttg acggttcgca agcggaaggt gctgccccct ccggtataat cccgcctcgc
     1501 gctacaaccc acctgcctcc tccccgatgc tcactttctg gatagtggcc gccctgctga
     1561 tcgtcgcggc gctcgccttc gtgctggtac cgctgctgcg gccgcgtgca cgctccggcc
     1621 cgaccgtgcg tgaagccaac ctcgccgtcc ttcgcggaca gcggcaggac atcgagaccg
     1681 acgtggcgct gggcctcctg cccagggacg cgcgcgaatc ggcgctcgcc gaactctccg
     1741 cgcgcgccga ggaggacctg gccgcgcccg gcgaatccct ccagggtccg ccttcccggc
     1801 cgtgggtcct cgccgcattc ttcgccgtcc tcgttcctgc ggttaccgtc ggcttctacc
     1861 tgtgggtcgg cacgccggcc gcgaccgatc cgaaggcgat gacggcaacg gccgccgatg
     1921 cgcagccggg ccagcaccag atcgaggaaa tggtcgtcgc gctcgagcag aagatgcgcg
     1981 accggcccga cgacgtgcag ggctggatgc tcctcgcgcg gtcctttgcc gccacgggca
     2041 agacggaaaa ggcgctggcg gcctacgccc acctggcgca gatcgcgccc caggacgcgt
     2101 cggtgctcgc cgactatgcg gatgcgctcg ggctctcgca ggggcgcagc ctcgccgggc
     2161 agcccaccga gctcgcgatg cgcgcccttc agctggaccc gcgccacccg aaggcgctgg
     2221 cgctcgccgc caccgccaag ctcaacggcg gcgacttcgc cgcgtcgctg ggttactggg
     2281 agcgcctgta tgccgtgctc ccgccgggat cggaagatgc aaccgaggtg cgcaacatca
     2341 tcgaggacgt tcgctcgcgc gcggccgccg ctggcaagcc ccagccgccg tcgaagattc
     2401 tcgccgcagc acctgccccg gctgcgccgg tcaacccggc gatgtccgca gcaccggttc
     2461 cgccgatggg agccgccccc gcgaaggccg cggccggcgg caagtccgtg tccggcaccg
     2521 tcaagcttgc cgattcgctg gccgggaagg ccgcccccgc ggatacgctc ttcatctacg
     2581 cgcgcgccga gtccggatcg cgcatgccgc tcgcgatcat tcgcggcggc gccggcgaat
     2641 tgcccaaggc cttcgtgctc gacgactcca tgggcatgtc gccggcggtg aagctttcca
     2701 cgacaccctc ggtggtcatc gaggcgcgcg tttcgaagtc cggcggcgcc gtaacccagc
     2761 cgggcgacct catcggcgtg agcaagccgg tcgcgccggg cgccaaggac gtggcgatcg
     2821 tgatcgacag gctcgttccg taaggcgccc gtgccgatga ttgcgtcgca ccctccccgc
     2881 cgatgaccgg aaaacaccgc agcggcgcgg cagacatcct ccaggtccgc gaactcgcct
     2941 gccgccgcgg gccggccctg ctcttctcgg ggctcgggtt cgaggtggca agcggggaac
     3001 tcctctgcgt gcgcggcccc aacggctgcg gcaagacgac gctgcttcgc tgcgtgacgg
     3061 ggcttacgcg gccggacgag ggaaccatcc tgtggcatgg cgaatcgtcc cggacggagc
     3121 ccgagcgcat gcgcgccgga accgcctacg cggggcacct gccggggctc aaggacgacc
     3181 tgacggccga ggaaaacctc gagttctcgc tgcgccttcg cggcacggct tccaccgccg
     3241 agcagcgtcg tgaagcgctg gccgccgtcg gcctcgaaaa gcgccgccgc ctgccggcgc
     3301 ggcggctttc cgccgggcag cgacggcgaa tcgggctggc ctggctctcg ctcgacccgg
     3361 ccgagttctg ggcgctggac gaaccgctca ccgcgctcga cgacgccgga caggcgctct
     3421 tcgtgggcct cctggaacgc cacctggcca acggcggcct ggcgctcgtc gccacccacc
     3481 accgcctcgc tccctcttcc ggcgtggtcc gcgagcttcg gctggcatga ggcgtttgca
     3541 cccatgaccg cgaatctcgg cgcactggcc ccggcacagg ggtcgttctc cggcggattc
     3601 gcctgggcga tggcgcgcga cctgcgcctc gcatggcggc atcccgacga ggcggtgctc
     3661 acggtggctt tcttcgcgct cgtggcctcg ctttttcccc tgggcatcgg agccgagccg
     3721 caactcctca ggcgcatcgg gccgggcgtg atctgggtgg gcgcactgct ggccgcgttt
     3781 ctcgccctgc ccggactttt cgccgcggat tacaccgatg gctcgctcga gcagatgacg
     3841 ctctcgccgc accccgcgtt ggggtggtcg gccggcaaga tcgcggccca ctggatcgtg
     3901 tcgggcgttc cgctcaccct gctttcgccc ctcatcgggc tgcaatacgg ccttgcgccc
     3961 gatacgctcg ccgttcaggc cctttcgctc gccataggca cgcctgtgct ttcacttctg
     4021 ggcggggcct gcgcggcgct cgccctcggg gcgcggggcg gcggcatgct gctggcgctc
     4081 ctcgcgctgc cgctcttcgt cccggtgctc atcttcggag ccggcgcggc ggaagcccag
     4141 gccaccggac tttctcccgc cccgcacctg tcgcttctcg ccgctgcgct gatcgccgcg
     4201 ctggtggggc ttccccttgc catcggggca gcggtgcgga tcgcggtgga ctaagcgtga
     4261 tgccggcccc tgccctttgc gttcccgcaa ccggtttccc cctgacccgg cgatacttcc
     4321 ggatcgagaa atcgccccca agcccatgaa ctggttctat ttctcctcgc ctccgacctt
     4381 ctaccgcctc gccgggcgcc tggcgccgtg gttcttcatc atggccgcgc tgctggcggc
     4441 ctggggcctg tacctcggcc tgctggtggc gcccacggat gcccagcagg gcgccgtgta
     4501 ccgggtgatc ttcatccatg tcccgacggc gtggatgtcg atgttcctgt acttcgtcat
     4561 ggcctgctac tgcgggctca cgctggtctt caacacgcga ctgtccgcga tgatggcgcg
     4621 cgccatagcg cccaccggcg ccatgctcac cttcatcgcg ctctggaccg gctcgttctg
     4681 gggtcgcccc acctggggaa cctactgggt gtgggacgcc cggctcacct cggaactggt
     4741 gctgctgttc ctgtacttcg gctacatggc gctggccaat gccttcgagg atccgcgccg
     4801 gggagaccgg gccgcagcgc tgcttgcgct catcggcgtg gtcaacctgc ccatcatcta
     4861 tttctcggtc cagtggtgga acacgctgca ccagggctcc agcgtgagct tcaagggcac
     4921 cagcatgcac gtgacgatgc tcaccggcat gctcgtgctg agcctcgcag cctggatgta
     4981 cgcgatcggc gcggcgttgg tgcgcgtgcg cacggtgatc ctggagcgcg agcggcgcac
     5041 ccaatgggtg cgtgagctgg gggccgcatg acgatctact ggtcgagctg gtcggaattc
     5101 tgggcgatgg gtggccacgc gttcttcgtg tggatgtcct acggggcact tctcgccgcc
     5161 atcattgtcg aaattgtctt gctgcgggtg cggcgcaagc gcgccctcgc tgccatcgag
     5221 gaggaacgcg atattgaagc cgagaactaa acgcgcgctc gccatcgccg gcgggctggc
     5281 cgcgctgggc gtggcggcgg cgctggtact gaatgccttc cagtcgaacc tggtgttctt
     5341 cttcacgccc acgcaggtgg ccaggaagga ggtgccgctg gaccgcacat tccgcatcgg
     5401 cggactggtc gagggcggct ccgtcgtgcg ccggaaggat gcgctttccg tcaccttcaa
     5461 ggtgaccgac ggcgcggaga cgatcccggt cgcctacgtc ggcatcctcc cggacctctt
     5521 caaggagggc aagggcgtgg tcgccgaagg gcgcgtgggc aaggacggca tcttcaacgc
     5581 ttcccaggtg ctcgccaagc acgacgagaa ctacatgccc cccgaagccg ccgaggcgct
     5641 caagcaggcg ggtcatccga tggccaagga aaacttcgca ggacaaggag ggatcaagcg
     5701 atgattcccg aaatcggcca gctcgcgctg gtgctggcac tctgcctcgc cgtcgcgcag
     5761 gcgttcctgc cgctcgccgg cgccgcgctc ggcaagcatt cgtggatggt aacgtcgcgc
     5821 cccatcgccc tgggccagtt cctgttcgtg ctgctcgcct ggacggcgct cgccacgtcg
     5881 ttcgtcaaca acgacttctc ggtagccaac gtcgccgagc actcgaacct cagccttccc
     5941 gcccactacc ggttcgccgc cacgtggggt tcgcacgagg gatccatgct cctctgggcg
     6001 ctgatgctcg gcgggtggac ggcggcggtg gcccttcgtt cgcgccacct ccccgagcgc
     6061 atcgccgcac gggtgatcgg gatcatgggc ctcatctcga cgggtttcct gctcttcatc
     6121 ctcaccacgt ccaacccctt cgacaggctg ctgccgccgg ccgcaaatgg ccgcgacctg
     6181 aatccgctcc tgcaggaccc gggcatggtg atgcacccgc ccatcctgta catgggttac
     6241 gtgggtttcg ccgtggcgtt cgcgttttcc atcgctgcgc tcctcgaagg aaggctcgac
     6301 agcacctggg cacggtggtc gcgcccgtgg accacggcgg catggtgctt tctctccgtc
     6361 ggcatcgcgc tgggcagctg gtgggcgtac tacgagctgg gctggggcgg ctggtggttc
     6421 tgggatccgg tcgagaacgc ctcgttcatg ccatggctcg tcggaaccgc gctcatccat
     6481 tcgctcgcgg tgaccgagaa gcgcggcgcc ttcaagagct ggacggtgct gctcgcgatc
     6541 ctggccttct cgctgtcgct gctgggcacc ttcctcgtgc gctccggcgt gctgacttcg
     6601 gtgcacgcgt tcgcgaccga tccgaggcgc ggtgtcttca tcctcacctt cctcgccatc
     6661 gtggtcggcg gctcgctggc gctcttcgcc tggcgtgcgc cgcgcgtggg catgggcggg
     6721 cagttcgccg gcgtgtcacg cgaatcgatg ctgctcgcga acaacgtcct tctcggcatc
     6781 gcgatgacgg cggtgctcct gggcacgctg tatcccctgg tgctcgacac cctgggcctg
     6841 ggcaagatct ccgtcggccc gccctacttc gacacggtgt tcttcccgct catggcgccc
     6901 gcggtcttcc tgatggccat cggcccgatg gcaagctgga agaaggctga actgcccgat
     6961 ctcgccacgc gcctgaagtg ggcccttggc gtggcactcg cgtgcgcagt gatagtcccg
     7021 tttgccaggg ggaaatggtc gcctctcgtg gtgctcggcc tcttcctcgc cttctgggtc
     7081 gtggccgcga cggcggtcca gttcggaacg cgcatcaagg cgtcgccaca ggccacgctg
     7141 atgggcaagc tgcgcgccaa ttccgcggcc tggtacggga tgcaggttgc ccacctcggc
     7201 gtggccgtct tcatcatcgg cgtcaccatg gtgaagggct tcgagacgga gcgcgacatc
     7261 cgcatggctc cgggcgagac ggccaccatc gcgggctacg agttcaagtt cacggcgacc
     7321 cgcgagattt ccggccccaa ctatggcgcg gtccgcggca cgttcgaagt gcgccgcgag
     7381 ggatcgccgg acgtgatccg gcgcatgtat ccggagaagc gcatgtacca ggcgtcgggc
     7441 cagaccatga cggaagccgc cattgccgcg ggcctgaccg gcgacctcta cgtgtcgctg
     7501 ggcgagcagg tggaaggcaa tgcatggggc gtgcgcatct accacaagcc cttcgtcgac
     7561 tggatctggg gcggatgcct gctgatggcc ttcggcggct tcctcgccat tgcggatcgc
     7621 cgctaccgcc tcgcgaaacg cacggccacc gtgccgggcg cgaccctcgc caccggagac
     7681 tgacgtggcg aaggcgctca agtacctcgt gccgctggcg atattcctcg tcctttgcgc
     7741 gttcctcgcc gtcgggctca ccaaggatcc gcgcgaggtt ccctctccgt tcatcggcaa
     7801 gcccgcgccc gccttcaggc tcgcgaagct ggacgagcct ccggcgagct tctcgcccga
     7861 ggacatgaag ggcaaggtat ggctgctcaa cgtgtgggcc tcgtggtgcg tgtcgtgccg
     7921 cgtcgagcac cccgtgctca acgacatgaa gcgcaggggc ctcgtcccga tcgtgggcct
     7981 gaactacaag gacgcgcgga cggacgcctc gaaatggctg cagcagcacg gcgacccgta
     8041 ccagctttcg gtgtgggact tcgagggcaa ggtgggcatc gactatgggg tctacggcgc
     8101 ccccgagaca ttcgtgatcg acaagcaggg gatcatccgc tacaagcaga tcggcccgat
     8161 cacgcccgag gccctcgaga agacgatcct gccgctcctc gcgaagctca acgcatgaag
     8221 cgcttcctgg tcctgctttt cctggctgcg tcattcgccg cgtggggaca gtcctccgaa
     8281 atcgcccatg ccgatcccaa ggtcgaggcg cgcctgaagc agatctccga ggagttgcgt
     8341 tgcctcgtgt gccagaacca gaccatcgcc gattccaatg cgccgctcgc cctcgacctg
     8401 cgccagcaga tccgcggcat ggtcggcgcc ggcaagaccg acgacgagat ccgtgcctac
     8461 atggtggagc gctacggcga cttcgtgctc tacaagccgc ccttcaacgc cgtcaccgcg
     8521 ctcctctgga tcggccccgg ggcccttgtc gttttcggct tcgccgcgct gtacctgatg
     8581 attcgccgcc gccgcgaggc agccggagcc gcgccccagc ccgacccggc gcgccgtcgc
     8641 gagatcgatg cgctcctcgt atcggacgac gagccgaaac cgaagccgaa cgcgaagcgc
     8701 taagagctgc ctagagcatc accggctggt cgggcaattc gttcgcgttg gccgcctcgg
     8761 ccgggaaatg cctctcgagc agcgctgaga tggcggtaac tccctcaact gcgccctccg
     8821 cgaaccgccc ggcgcgaaac gcctcctgca tcgaagcgca gattgcctgc cattcggctt
     8881 gcgcaaccct ggccgagatg ccccgatcgg ccacgatctc caccttccgg tctgccagga
     8941 gcacatagac cagcacgccg gtattttccg cggtgtccca tacgtggagc atcgcgaaga
     9001 gctcgacggc gcgcgcgcgc gcgccgagat tggcccagag ctgcgccgag gtgagttcgg
     9061 cctccaccac gaagcgaatc tcgccgcgat gctgcttctc gtgctctgcc accgcgcggc
     9121 cgatggcgcc gcgcgccgag ggcgggaagg cacgcgccgc cttccagggg gtcatcgcga
     9181 tgtgccgcca gaatcgtcgc aggtccatca ccagttcccc gaagcgccgc cgccgtcgaa
     9241 gccgccgcca ccgccgctga agccgccacc gccgccgccg aaaccgccgc caccgccacc
     9301 cgaccacccg cctccgcccc acggaccgcc gccgatgcca ccgcgccgcg cgcccagggc
     9361 cgacgtgccc atgaagagca cggcgaggaa agtgatgacg gcggcaatgc cgcccagcaa
     9421 gaggctgccg aagatgaccc acgccgccag ccccgtgacg cccgaggtga gcccggcgcc
     9481 gaggaagcgc cccacgagcg agcgcaggat catgccgacc accggcacga cgacgaaggc
     9541 aagccagaag aaattctcga tcgacgagaa cgagtcgacc ttgccgcgcg acggcgcctg
     9601 cgcgggcggc gggagcgact cgccctcgat cgccttcatg atcgcgtcca cgccatccga
     9661 gataccaccc gcgaagtcgc cggcgcggaa ccgcggcgag acgcgctcgg cgatgatgcg
     9721 acgcgagaga gcgtccgtga ggacgccctg cacgccacgc cccgtctgga tgcgcatgcg
     9781 ccgttcctgc ttcgcgatga cgaagaggac gccgtcatcc acgccggcgc gcccgagctt
     9841 ccattcgtcg gtgacgcgcg tcgcgaattc ctcgatggtc tcggtgccga tggccggcac
     9901 catcagcacc gccacctgcg agcccttcgc cgcctcgaac gccttcagcc tggcggcaat
     9961 cgcctcgctt tcgggcgcgg ccagcgtcgc cgtgaggtcg atgacgcggc cggtgagctt
    10021 cgggaccggg atgggctccg tcacgtccgc ggccagcgcg gcgaacgcga cgaagaagga
    10081 cgcggccgcc cctgcgagga ggcggccggc gatacgcgtc acttggacgg tgctggctgg
    10141 gccggggcgg ccggttgcgc cggcgctgca gcgggcttgg ccgagaagtc gaccttgggc
    10201 ggctcggaga tctgcttttc gttttccacg gtgaagctcg ccttttcctt gtagccgaaa
    10261 agcatggccg tgagattggt ggggaactgg cgcgcgagga tgttgtagcc ctgcaccgtc
    10321 ttgatgtagc ggttcctcgc cgtggtgatg cggttctcgg tgccttcgag ctgcgactgc
    10381 aagtcgcgga aattggcgtc gctcttgagt tgcggatagt tttccacgac caccatgagg
    10441 cgcgaaaggg cgctcgacag ctcgccctgg gcggcctgga agttcttcat ggcattcggg
    10501 tcgttgagcg cctcgggcgt catctggatc gagcccacct ttgcgcgtgc gttggtcacg
    10561 cccagcagca cgtccctctc ctgtgcggcg aagccctgca cgacgctcac caggttgggg
    10621 atcaggtccg cgcggcgctt gtactggttg agcacctccg cccagccggc cttcacttcc
    10681 tcgtccttcg tctggaagtc gttgtagcca cagccggata gggcgagcgt gccggcgagc
    10741 gcgaatgcgg tcaggaactt gcgcatgaaa tcgaaacctc ccgtttgggg ttggaatgca
    10801 tggccaatgg tggggctggc ggctgccgga ttcaagggcc gcgcgggacg gtcacgcctg
    10861 cgccgccgcg aaagtgcgcc tggcgaaaag gaggtcctcc cacgccttcg ccttgtcggg
    10921 gaggttgcgc aggagatagg ccggatggta ggtgatgacg agcgggatgc cgcggtagtc
    10981 gaggaccctg ccgcgctggc tggcaaggct cgactcgttt ccggtgagcc gcaccatggc
    11041 ggtcttgccc atcgcgacga tgagcttcgg gcggatgagg tcgatctggc ggtcgaggaa
    11101 gggcgcgcac gcggcggcct cttccggcgc cggcacgcgg ttgcccggcg ggcggcactt
    11161 caccacgttg gcgatgtaga cctcgcgtcc gcgcgcgagc ttcgcggcgg ccagcatgct
    11221 gtcgagaagc ttgcccgcct gcccgacgaa cggctcgccc ttctcgtcct cgtccgcacc
    11281 cggcccctcg ccgacgaaga gccacggcgc tgcgacgttt ccgacgccga acaccgcctg
    11341 cttgcgctgc tcgcacagga cacatcgacg gcagctgcgc acggtctctt cgagagcggc
    11401 ccagtccatc gcctcgatgc cggccactgg cggcgaggca gccggcgccg gcgcgcggag
    11461 agccgcccgt gccggcccgt tgcccggcct cgcggcgggc ggagtctcga cagtgggcgc
    11521 ctcggacgct gctgcccgaa cggccggcga ggccagcccg tacagacggg cgacgggcag
    11581 aagctcgagt tccctgagga ttcggtcgtc catggctcag agggtgatgc cgaggaacag
    11641 cgcatcttcg cgtcccccgc gcgcgggata gtaggacggg cggatggcga tctgctggaa
    11701 gcccatctcg cgataaaggt ggcgcgcggc caggttcgac ggtcgcacct cgaggtacac
    11761 gatctggcac ttcgactccc gcgccgtgcc cagcatgtgg tcgaggaagg cacgcccgat
    11821 gccctggttc tgccagcctg tcgcgacggc gaagttgagg aggtgcgcct cgtcgagcgc
    11881 gaccatgagg atcgcatagc cgatcacgtg ttcgccgctg caggcgaccc agcagtcgta
    11941 acccgcgttc accgaatcgc ggaaattgcc gaggctccag gggaaggcat agagggactt
    12001 ctcgagatcg gcgacaacgc ccaggtcggc ggggcacatg cgccgatagt tcagctgcac
    12061 ccggggtagc gcgctcactt cgcagcctgc ctttcgcgtg tcgtcaaggc gaccttgtcg
    12121 cgcaagtata ggggcgcggc ttcctcggcc gcgagcgcct cgccgcgggc cagctttcgc
    12181 gccgcgacgc gggccacgga ccctgcccgg ggaaggtcgt tctcaatccg atggccgact
    12241 tgcggggcgt acgccgagcg aagccagccg aaggcatcga aaccgctgcc cgtggccacc
    12301 cagtcacgcc ccggaagcgc cggcagggac ccctgggaat gcagacccgg ctcgatggcg
    12361 gcctcccagt cgccacccat gcgcgaatag gccgcgaggt aggtctcgcc catgcgggcg
    12421 tccagcccca ccagcacgcg actcgcgttg gcctgctcgg caagtgcgac cagggatggg
    12481 accggtacga ccgggcggga ggcgccgagc gc
//
LOCUS       DASUEV010000360        11297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_333957, whole genome shotgun sequence.
ACCESSION   DASUEV010000360 DASUEV010000000
VERSION     DASUEV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333957"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /gene="malQ"
                     /locus_tag="VFV55_11450"
     CDS             <1..2302
                     /gene="malQ"
                     /locus_tag="VFV55_11450"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX4944961.1"
                     /translation="HARDGFQWRKAVDTALPGVAPAALVDEAHDALAARAVAVLVEEA
                     QAAPPRRQTGIEPDVLDRLSLAAGIARTWHDVAGTEHVVGDDTRRALLAAMRLDATST
                     GQARERLHELAAARECRHLPRVIVLHERGPLRIPLAANPSQAGPGILRIVASDGEVRR
                     TPFDPAHGTVDRVVACDGRTVARHLVALPPLPEGTYTVSVEGEPLACRLVVAPRRCFL
                     PSALLDGKRRFGLAAHLYALRRPGDQGIGDFTALAQAAEATARAGGSLVGINPLHALF
                     AAQRDRASPYHPSDRRFLEALYIDVAAIPDLADSDVARRALEQHMALLGRRAAQPAVD
                     YAVVWAGKRPVLEACFEAFSRRPAGDALAAEYGRFVADGGERLRRFAAYEAIAATRPG
                     VPWQRWPGELRRPDDPGVARFAARHAREVDFAMYLQWLADRQFGQAARSARAAGLGIG
                     FYRDLAIGAAPDGAEAWDTQATLAHGASVGAPPDPFCAEGQVWSLPPPLPEALAAEGY
                     EGFRTLIAGNARHAGALRIDHVMGLSRLFWVPDGATAADGAYVRYPLDDLLGVLALES
                     RRAHCLIVGEDLGTVAEGLRERLDSAGVLSYRVLWFERDGAQFRGPQRYPAKAAACIS
                     THDLPTIRGWWDGADIDERRALGLAGDDETATARATRIADRNALMRAMDAAGVSAPAA
                     ELAAGPEVAVAQALHRFIGATPCALALIQADDLAGETSAPNLPGTDRERPNWRRRLAV
                     DAGNLWATPIGAQAARDLAPARGTAT"
     gene            complement(2295..3269)
                     /locus_tag="VFV55_11455"
     CDS             complement(2295..3269)
                     /locus_tag="VFV55_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HEX4944962.1"
                     /translation="MKHLFKAALVASVLALAAGGAIAQTKLKFAHVYETSEPYHAAAL
                     WAAGEIAKRTNNRYAVEVFPASTLGKESDINQGLTLGTVDIIYTGQLFTGRTYGPLAI
                     GGAPYMFRDFAHWKAFTTSALFSELAEGYRQKSGGNKVVAITYYGERHVTSNKPINKP
                     EDMKGLKIRVPDAPLYTMFPRAVGANPTPIAFAEVYLALQNGTVDAQENPLPTIDAKK
                     FYEVQKYIVLTGHITDALLTIVGAPTWNKLSDADKKTFEAVLREAAARATAEIIESEK
                     KMVAEFEKRGKTVVRVDRKPFRDMTVKLHNGPDASWPKDIYDKLQAIK"
     gene            complement(3309..4832)
                     /locus_tag="VFV55_11460"
     CDS             complement(3309..4832)
                     /locus_tag="VFV55_11460"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX4944963.1"
                     /translation="MLRETPLYLRLNPADDVVIACRELEAGLNLVKEGVIVAERIPSG
                     HKVATRAIAVNEPVRRYDQVIGFATKPIAKGGHVHVHNLEVRDFARDYAFGEAYRPTQ
                     FVAQPATFQGIVRADGRVATRNYIGILSTVNCSATVSKYVAEAFRGDALKEFPNVDGV
                     VALTHGTGCGMGSTGEGIDLLRRTIAGYARHANFAGILVIGLGCEANQMDALFFTQGL
                     AAGPLLRSMTIQDEGGTAKCVAQGIAAVREMLAHANLVRRETVPAGHLLVGLQCGGSD
                     GYSGITANPALGAAVDLLVRHGGTAILSETPEIYGAEHLLTRRAVTRAVGEKIVERIR
                     WWEGYTAKNDGEMNNNPSPGNKAGGLTTILEKSLGAVAKGGTTGLMGVFEYAYPVTAK
                     GMVFMDTPGYDPVSATGQVAGGANLICFTTGRGSVYGCKPAPSLKLATNTPLFQRMEG
                     DMDVNCGEILDGSLSIEEAGRRIFELMLATASGQPSKSERHGFGADEFVPWQLGAVM"
     gene            complement(4854..6257)
                     /locus_tag="VFV55_11465"
     CDS             complement(4854..6257)
                     /locus_tag="VFV55_11465"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX4944964.1"
                     /translation="MGPGTFKLLFLSFMGAGVPVAIAMAGSSLVYVMVTGITPDYVVI
                     HRMVGGLDSFPLLAVPFFIFAGNLMNSAGITNRIYNFALGLVGWLKGGLGHVNVLGSV
                     IFAGMSGTAIADAAGLGTIEIKAMQDHGYPTEFAVGVTAASATLGPIIPPSLPFVIYA
                     MFANVSVGSLFLAGILPGAVLAFLMMCTVAFYAHRRGWGRDVPLDWRRIGRAFAELAV
                     VAAFPVVVWAAVELGADPQWAVVFTLALLLAVDEYFHFSAVLPIMTPVLLIGGMTAGI
                     FTATEGAIAACVWALFLGLVRYRAMTWRMLVKVSMDTIETTSTVLLIVAAASIFGWML
                     TATRTTEMIAAWVFSVTTDPNMFLLIANLLMLFVGCFLEPTAAITILVPILLPIVHKL
                     GIDPVHFGLVMVLNLMIGLLHPPMGLVLFVLARVAKLSVERTTMAILPWLVPLLVSLG
                     LVTYIPSISLWLPNALK"
     gene            complement(6258..6833)
                     /locus_tag="VFV55_11470"
     CDS             complement(6258..6833)
                     /locus_tag="VFV55_11470"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX4944965.1"
                     /translation="MTAVEIAQEEEHILNEKGEFEIHDRPVDISHYRFEDWLSFAFFW
                     VLALVIFYQFFTRYVMNDSASWTEEIARYLLICVAFVGAAVGVRRNNHIQVDFLYRYL
                     PHAACRAMSTLVDVLRIAFLGYCVWLTYLLMGKIGSSRMSIVDLPMGLVYGVVLFGFA
                     LMTWRAIGTARASWRRGASVLERPEIADEAK"
     gene            complement(6830..7549)
                     /locus_tag="VFV55_11475"
     CDS             complement(6830..7549)
                     /locus_tag="VFV55_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX4944966.1"
                     /translation="MPLQAVENRRLYRQIADQIAALIEKGEYASGGRLPPERDLARQL
                     GVSRPSVREALIALEVEGYVDVRVGSGVYVQRSRPAAKAAPLSSDTGPFELIRARWLI
                     EGECAALAAKEATKAQVRSMEDALEAMEAVQGDGDRLVDDRLFHLRVAEGSGNSALAL
                     AVQTLWDQRMGPLFLQLEQHFDTPELWGVAIREHREILAAIQKRDAAGARAAMRSHMN
                     HAARRFSTSWGKKKSRRGGKR"
     gene            7631..8371
                     /locus_tag="VFV55_11480"
     CDS             7631..8371
                     /locus_tag="VFV55_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX4944967.1"
                     /translation="MANRLQGKTALVTAAAQGMGRAGALAFAREGAKVIATDIDMAAL
                     ASLSPTPGITVKRLDVLNDAEVRSFVEATGAVDILFNCAGWVHQGSLLDCSVEDWDRS
                     FALNVRSQFVMVKAMLPKMAAAGGGVILNMASVLGSDKAAPNRLAYAASKAAVAGFTR
                     ALAIDHVKQNIRVNCVCPGTVDTPSLGERINAFADPVQARKDFVARQPMGRLATAEEL
                     AETFVYLVSDESSFMTGQAVFVDGGMSL"
     gene            8506..9369
                     /locus_tag="VFV55_11485"
     CDS             8506..9369
                     /locus_tag="VFV55_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX4944968.1"
                     /translation="MKLLRYGPPGREKPGLCDDGGRIRDLSKVIVQLDDAMLSPRNLA
                     KLARIDPESLPIVRGSPRLGVPYAGISKFVAVGLNYTDHAAESNMPIPKEPILFMKAT
                     TSLCGPNDNVIQPKNSTKLDWEVELGIVIGTKAQYVPEAKALDYVAGYCVVNDVSERD
                     FQLERGSQWDRGKGCDTFGPVGPWLVTTDEITNPQSLEMWLDVNGRRRQRGNTRTMIF
                     GCANLVSYISRFMTLLPGDIITTGTPPGVGLGMKPPQYLTVGDTITLGIQGLGQQKQN
                     VVAWTPGSGFK"
     gene            9366..10415
                     /locus_tag="VFV55_11490"
     CDS             9366..10415
                     /locus_tag="VFV55_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX4944969.1"
                     /translation="MTVRVAHGPLTQWAVACLRAVGVPEEDAKTVGGCLVQTSLWGID
                     SHGVLRLTHYLTRLSKGSIVADARPVVTRTGPVTAQVDGRDGLGIAHAVLAMSVAIEL
                     AKDAGIGIVGVGNSSHCGAMGLYTREAARQGLIGIAMTHSSSVVVPHGGRHKYFGTNP
                     ISFAFARQGGEPVCLDMATSQVAWNKVLNARIEGDTLEPGLAVDENGEPTTDPHLARA
                     GIALGGPTYGYKGYGLAFMIELLCGPLNAMTFGPRVNDMYGQLDRPRRIGHFLMALDP
                     QRFAGGPMLAATVDEIVRDLKTRGEILHPGEPELIHERERLASGIPIEPVAYADMVAW
                     SDKLGVAAPAPVSGT"
     gene            complement(10377..10961)
                     /locus_tag="VFV55_11495"
     CDS             complement(10377..10961)
                     /locus_tag="VFV55_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX4944970.1"
                     /translation="MGTVGEVIPIRARMHVVETDTVTLEPQTAAHAQEMFAVLGDPAL
                     YEHENEPPPSLDWLRERFARLESRMSADGREHWLNWVVRLPASGLIGYVQATVHPDGR
                     AAIAYVLSSAYWGRGLARHAVEAMIAELVENCGVRSLSAVLKRGNHRSLRLLERLGFT
                     LASPGQHVERQVAPDELLMQRELGARDRSGRGHA"
     gene            complement(10940..>11297)
                     /locus_tag="VFV55_11500"
     CDS             complement(10940..>11297)
                     /locus_tag="VFV55_11500"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX4944971.1"
                     /translation="AGLATVGLLGTRFTMEEDFYRERLRERHGLQVLIPEWEDREIVH
                     RVIYDELCLGKVLPESRAEYRRIMAELARQGAQAIILGCTEISLLVDQEDSDVPLFDT
                     TGIHARRAAEWALSGR"
BASE COUNT         1794 a         3932 c         3846 g         1725 t
ORIGIN      
        1 tcacgcgcgc gacggcttcc agtggcgcaa ggccgtggat acggcattgc ccggcgttgc
       61 cccggctgca ctcgtggacg aagcgcatga cgccctggcc gcgcgagcgg tggcggtcct
      121 cgtcgaggaa gcacaggccg caccgccccg gcggcaaacc ggcatcgagc ccgacgtgct
      181 cgaccgactg tcgctggctg ccgggatcgc gaggacctgg cacgacgtcg cgggaacgga
      241 acacgtcgtc ggcgacgata cgcggcgtgc gctcctcgcc gcgatgcggc tcgatgcgac
      301 ctcgaccggt caggcccgcg agcggctgca cgaactcgcc gccgctcgcg agtgccgcca
      361 cctgccgcgc gtgatcgtgc tccacgaacg cgggcccctg cgcatcccgc tggccgcgaa
      421 cccctcgcag gccgggccgg ggatcctgcg catcgtcgca agcgacggag aggtgcggcg
      481 cacgccgttc gatcccgcgc atggcaccgt tgaccgcgtc gtggcctgcg atggacgcac
      541 ggtcgcccgg cacctggtgg cgcttccgcc gttgcccgag ggcacctaca cggtgagcgt
      601 cgagggcgag ccgctggcct gccgcctcgt cgtcgcgccg cgccgttgct tcctgccctc
      661 ggccctcctc gacgggaaac gccgcttcgg cctcgccgcg cacctgtacg cgctgcgcag
      721 gccgggcgac cagggcatcg gcgatttcac cgcgctcgcg caggcagcgg aagccaccgc
      781 gcgcgccggg ggctcgctcg tcggcatcaa tcccctgcat gcgcttttcg cggcgcagcg
      841 cgaccgggcg agcccctatc acccgtcgga ccgccggttc ctcgaagccc tctacatcga
      901 tgtcgcggcg atcccggatc tcgccgattc cgacgtggcg cgccgtgcgc tggaacagca
      961 catggcgcta ctcggccgcc gggcagcgca gcctgccgtc gactatgccg tcgtgtgggc
     1021 ggggaagcgg ccggtcctgg aggcgtgctt cgaggccttc tcgcgacgac cggcgggaga
     1081 tgccctcgcc gccgagtacg ggcgcttcgt ggccgatggc ggggagaggc tgcgccggtt
     1141 cgcggcgtac gaggccatcg ccgccacgcg gccgggcgtg ccgtggcaac gctggcccgg
     1201 ggaattgcgc cggcccgacg acccgggcgt ggcgcgtttc gccgcgcgcc acgcgcgcga
     1261 agtggatttc gcgatgtacc tgcaatggct cgcggatcgc cagttcgggc aggccgcgcg
     1321 gtcggcgcgc gcggccgggc tcggcatcgg gttctatcgc gacctcgcca tcggcgccgc
     1381 gcccgatggg gcggaggcct gggacacgca ggccacgctc gcgcacggtg cgtcggtcgg
     1441 cgcgcctccc gatcccttct gcgcggaagg gcaggtctgg agcctgccgc cgccgcttcc
     1501 cgaagcgctc gccgccgagg gctacgaggg gttccgcacg ctcatcgccg gcaatgcgcg
     1561 ccacgcgggc gcgctgcgca tcgatcacgt catgggcctt tcacgcctct tctgggtgcc
     1621 cgacggcgcg acggcggccg atggcgccta tgtccgctac ccgctggacg acctcctcgg
     1681 cgtgctggcc ctcgagagcc gacgcgcgca ctgcctcatc gtcggcgagg acctgggtac
     1741 ggtggccgag ggcttgcgcg agcggctcga ttccgccggc gtgctctcgt atcgcgtgct
     1801 gtggttcgag cgcgacggcg cgcaattccg cggtccgcaa cgctacccgg cgaaggccgc
     1861 ggcgtgcatc tcgacgcacg acctgccgac gatccgcgga tggtgggacg gcgccgacat
     1921 cgacgaacgc cgcgcgctcg gtctcgccgg cgacgacgag acggccacgg cacgggccac
     1981 gcggatcgcg gaccggaacg cgctcatgag ggccatggac gcggcgggcg tttcagcccc
     2041 cgccgcggag ctcgcggccg ggcccgaagt cgcggtggcg caggccctgc atcgcttcat
     2101 cggcgccacg ccttgcgcgc tggcgctcat ccaggccgac gatctcgccg gagagacatc
     2161 cgcgcccaac ctgcccggaa ccgaccgcga gcgccccaac tggcggcgca ggctcgcggt
     2221 cgatgccgga aacctgtggg ccacgcccat cggggcgcag gccgcgcggg acctggcgcc
     2281 cgcacgcggc acggctactt gatcgcctgc agcttgtcgt agatgtcctt gggccaggag
     2341 gcatccgggc cgttgtgcag cttcaccgtc atgtcgcgga agggcttgcg gtccacgcgc
     2401 accaccgtct tgccgcgttt ctcgaactcc gcgaccatct tcttctcgct ctcgatgatc
     2461 tccgccgtgg cacgcgcggc cgcctcgcgc aggaccgcct cgaaggtctt cttgtccgca
     2521 tccgagagct tgttccacgt cggcgcgccg acgatcgtca ggagcgcgtc cgtgatgtgg
     2581 ccggtgagga cgatgtactt ctgcacctcg tagaacttct tcgcgtcgat ggtgggcaag
     2641 gggttttcct gggcgtcgac ggtgccgttc tgcagcgcga ggtagacctc ggcgaaggcg
     2701 atcggcgtcg ggttcgcccc caccgcgcgc gggaacatcg tgtagagcgg cgcgtcgggc
     2761 acgcggatct tgagcccctt catgtcctcg ggcttgttga tgggcttgtt ggacgtgaca
     2821 tggcgctcgc cgtagtaggt gatggcgacg accttgttgc cgccgctctt ctgccggtag
     2881 ccctcggcga gctcgctgaa gagcgcgctc gtggtgaagg ccttccagtg cgcgaagtcg
     2941 cggaacatgt agggcgcgcc gccgatggcg agcggaccgt aggtgcgccc ggtgaagagc
     3001 tgtccggtgt agatgatgtc cacggtgccg agcgtgaggc cctggttgat gtcggactcc
     3061 ttgcccagcg tcgaggccgg gaacacctcg accgcgtagc gattgttcgt ccgcttggca
     3121 atctctcccg ccgcccacag cgctgccgcg tggtacggct cgctcgtctc gtacacgtgc
     3181 gcgaacttga gcttcgtctg ggcaatcgcc cctcccgccg ccagcgccag cacggacgcc
     3241 acgagtgctg ccttgaacag gtgcttcatc gtcttctcct cctctgttat cggggtgccg
     3301 ctacaggatc acatcaccgc gccgagttgc cagggcacga attcgtcggc gccgaaaccg
     3361 tggcgctcgc tcttgctcgg ctgccccgac gcggtcgcga gcatcagctc gaagatgcgc
     3421 cggcctgcct cctcgatgga gagcgagccg tcgaggatct cgccgcagtt cacgtccatg
     3481 tcgccttcca tgcgctggaa gagcggcgtg ttggtggcga gcttgagcga gggcgccggc
     3541 ttgcatccgt acaccgaccc gcgcccggtc gtgaagcaga tgaggttggc gcctcccgcg
     3601 acctgcccgg tggccgagac cgggtcatag ccgggcgtgt ccatgaacac catgcccttc
     3661 gcggtcacgg gataggcgta ctcgaagacg cccatgaggc ccgtggtgcc gcccttggcc
     3721 acggcgccga gggacttttc caggatcgtg gtgagcccgc ccgccttgtt gccgggcgag
     3781 gggttgttgt tcatctcgcc gtcgtttttc gcggtgtagc cctcccacca cctgatgcgc
     3841 tcgacgatct tctcgccgac ggcgcgagtg accgcccggc gcgtgagcag gtgctcggca
     3901 ccgtagatct ccggcgtctc ggaaaggatg gccgtgccgc cgtgccgcac gaggaggtcc
     3961 accgccgccc cgagcgccgg gttcgcggtg atgcccgaat agccgtccga gcctccgcat
     4021 tgcaggccca cgaggaggtg gcccgccggg acagtctcgc gccgcacgag gttcgcgtgc
     4081 gcaagcatct cccgcaccgc cgcgatgccc tgggccacgc acttggccgt tccgccctcg
     4141 tcctggatgg tcatggaacg caggagcggt cccgcggcga gcccctgggt gaagaagagc
     4201 gcatccatct ggttcgcttc gcaccccagc ccgatcacga ggatgccggc gaagttggcg
     4261 tgcctcgcgt acccggcgat cgtgcggcgc aggaggtcga tgccctcccc cgtcgagccc
     4321 atgccgcagc ccgtgccgtg ggtgagcgcg acgacgccat cgacgttcgg gaactccttc
     4381 agcgcgtcgc cgcggaacgc ctccgccacg tacttcgaca cggtggccga gcagttcacc
     4441 gtggagagga tgccgatgta gttgcgcgtg gccacccggc cgtcggcacg cacgatgccc
     4501 tggaaggtcg ccggttgcgc cacgaactgc gtgggccggt acgcttcccc gaacgcgtag
     4561 tcgcgggcga aatcgcgcac ctcgaggttg tggacgtgca cgtgcccgcc cttggcgatg
     4621 ggcttcgtgg cgaaaccgat cacctggtcg tagcggcgca cgggctcgtt cacggcaatg
     4681 gcgcgcgtcg ccaccttgtg cccggagggg atgcgctcgg cgacgatcac gccctccttc
     4741 accaggttga ggcccgcttc cagctcgcgg caggcaatga cgacgtcgtc ggcggggttg
     4801 aggcgcaggt agagcggagt ttcgcgcagc atggtcggtc tttcaggcgg ccgtcacttg
     4861 agggcgttcg gcagccacag gctgatggag gggatgtacg tcaccagccc caggctcacg
     4921 agcagcggga cgagccaggg caggattgcc atggtagtgc gttccacgga cagcttggcg
     4981 acccgtgcga gcacgaacag cacgagtccc atgggcggat gcagcaggcc gatcatgagg
     5041 ttgagcacca tcaccagccc gaagtgtacg gggtcgatgc ccagcttgtg cacgatgggc
     5101 agcaggatcg ggaccaggat ggttatcgcg gccgtgggct cgaggaagca gcccacgaac
     5161 agcatgagga ggttggcgat cagcaggaac atgttcggat cggtcgtcac ggagaacacc
     5221 cacgcggcga tcatctccgt ggtgcgcgtg gcggtgagca tccagccgaa gatcgatgcg
     5281 gcggcgacga tgagaagcac ggtcgaggtg gtctcgatgg tgtccatcga caccttcacg
     5341 agcatgcgcc aggtcatcgc gcgatagcgg acgaggccga gaaacaacgc ccagacgcac
     5401 gcggcgatgg ccccctccgt ggcggtgaag atccccgccg tcatgccgcc gatgaggagc
     5461 accggcgtca tgatcgggag caccgcgctg aagtggaagt actcgtcgac cgcgaggagc
     5521 agcgccaggg tgaagaccac cgcccattgc gggtccgccc ccagttcgac cgcggcccaa
     5581 accaccacgg gaaaggcggc caccacggca agttccgcga aggcccgccc gatcctgcgc
     5641 cagtcgagcg gcacgtcgcg cccccagccc ctgcgatgcg catagaaggc caccgtgcac
     5701 atcatcagga aggccaggac ggcgcccggc aggatcccgg cgaggaagag cgaccccacc
     5761 gagacgttgg cgaacatggc gtagatcacg aacggcagcg acggcgggat gatcgggccc
     5821 agcgtggccg aggccgccgt cacccccacc gcgaactccg tggggtagcc gtggtcctgc
     5881 atcgccttga tctcgatcgt gcccagcccc gccgcatccg cgatggccgt gcccgacatc
     5941 cccgcgaaga tcaccgaccc cagcacgttc acgtgcccca ggccgccctt gagccagccc
     6001 acgagcccga gcgcgaagtt gtagatgcgg ttggtgatcc cggccgagtt catcaggttg
     6061 cccgcgaaga tgaagaaggg aaccgccagc agcgggaacg aatcgagccc gcccaccatg
     6121 cggtggatca ccacgtagtc cggcgtgatc ccggtcacca tcacgtacac gagcgaggag
     6181 cccgccatgg cgatggccac gggcacgccg gcgcccatga aggaaaggaa caggagcttg
     6241 aaggtgcccg ggcccatcta tttcgcctcg tccgcgattt ccggccgctc gaggaccgac
     6301 gccccgcgcc gccagctggc ccgcgccgtg ccgatggcgc gccacgtcat gagcgcgaag
     6361 ccgaacagca ccacgccgta cacgagcccc atcggcaggt ccacgatcga catgcgcgac
     6421 gagccgatct tgcccatgag gaggtaggtg agccagacgc agtagccgag gaaggcgatg
     6481 cgcagcacgt ccaccagcgt ggacatcgcg cggcaggcgg catgcggcag gtaccggtag
     6541 aggaagtcga cctggatgtg gttgttcctg cgcaccccga cggccgcgcc cacgaaggcc
     6601 acgcagatga ggaggtaacg cgcgatctcc tccgtccagg aggccgagtc gttcatcacg
     6661 tagcgcgtga agaactggta gaagatgacc agcgccagca cccagaagaa ggcgaaggag
     6721 agccagtcct cgaaacggta gtgcgagatg tccaccggcc ggtcgtggat ctcgaactcg
     6781 cccttctcgt tgaggatgtg ctcctcttcc tgcgcgattt cgacggcggt catcgctttc
     6841 ctccgcgccg ggatttcttc ttgccccagc tcgtgctgaa tcgccgcgcc gcgtggttca
     6901 tgtggctgcg catggcagct ctcgccccgg cggcatcgcg cttctggatc gccgccagga
     6961 tctcgcgatg ctccctgatg gccacgcccc acaattcggg ggtatcgaaa tgctgctcga
     7021 gctgcaggaa gagcggcccc atgcgctggt cccagagcgt ctgcacggcg agcgcgagcg
     7081 cgctgttgcc gctgccctcg gccacgcgca ggtggaaaag ccggtcgtcc acgagacggt
     7141 cgccatcgcc ctgcaccgcc tccatcgctt ccagcgcgtc ctccatcgag cgcacctggg
     7201 ccttcgtggc ttccttcgcc gcgagcgccg cgcattcgcc ctcgatgagc cagcgggcgc
     7261 ggatcagctc gaaggggccg gtgtccgacg acaggggcgc ggccttcgcg gcaggacgcg
     7321 agcgctgcac gtagacgccc gagccgaccc gcacgtcgac atagccttcc acctcgaggg
     7381 cgatgagcgc ctcgcgcacc gagggccggc tcacgccgag ctgccgggcg aggtcgcgct
     7441 ccggcggcaa gcggcctccc gacgcgtatt cccccttctc gatgagggcc gcgatctggt
     7501 cggcgatctg ccggtagagc cttcgattct cgactgcctg gaggggcatg tggtaaagtg
     7561 gtcaggccaa ttcagggcag cttttcacag gggcgcaccc ctgtcaagaa aaacgccggg
     7621 agcgatcgcc atggcaaacc gactgcaggg aaagaccgcg ctcgtcaccg cggcggcaca
     7681 aggcatgggg cgtgcgggcg cgctcgcctt cgcccgcgaa ggcgcaaagg tcatcgcgac
     7741 cgacatcgac atggccgcgc tcgcttcact ctccccgacg cccgggatca ccgtgaaacg
     7801 gctcgacgtg ctgaacgacg ccgaggtgcg ctcgttcgtc gaggccaccg gcgcggtgga
     7861 catcctcttc aattgcgcag gctgggtgca ccagggctcg cttctcgact gcagcgtcga
     7921 ggactgggac cgcagcttcg ccctcaacgt gcgcagccag ttcgtgatgg tgaaggcgat
     7981 gctgccgaag atggccgcgg ccggcggggg cgtgatcctc aacatggcct cggtcctggg
     8041 cagcgacaag gccgcaccca accgcctcgc ctacgccgcg agcaaggccg ccgtggccgg
     8101 gttcacgcgc gccctcgcga tcgaccacgt caagcagaac atccgcgtga actgcgtctg
     8161 ccccggcacc gtcgacacgc cttccctcgg cgagcgcatc aacgccttcg ccgacccggt
     8221 gcaggcgcgc aaggacttcg tcgcccggca gcccatggga cgcctcgcca cggcagaaga
     8281 gcttgccgag accttcgtct acctcgtttc cgacgagtca tcgttcatga ccgggcaggc
     8341 cgtgttcgtg gacggaggca tgagcctctg acggcctgtg caaagcggaa aaggggtcag
     8401 gttacttttc tgtcgtattg ccgtcgcaag acggcaggct gaaaagtaac cggacccctt
     8461 ttccacctga ccccttttcc accgaaaatt cgaggaggag gaaacatgaa actgctgcgc
     8521 tacggcccgc cgggccgcga gaagcccggc ctgtgcgatg acggcggacg catccgcgac
     8581 ctatcaaagg tcatcgtcca gctcgacgac gcgatgctct cgcccaggaa cctcgcgaag
     8641 ctcgcccgca tcgatcccga atcgctgccg atcgtgcgcg gcagcccgcg gctgggcgtc
     8701 ccttacgcgg gaatcagcaa gttcgtggcc gtgggcctca actacacgga ccacgccgcc
     8761 gagtcgaaca tgccgatccc gaaggaaccc atcctgttca tgaaggccac cacgagtctg
     8821 tgcggcccca acgacaacgt gatccagccg aagaactcca ccaagctcga ctgggaagtg
     8881 gagctgggca tcgtgatcgg aacaaaggcg cagtacgttc cggaggcgaa ggcgctcgat
     8941 tacgtcgccg gctactgcgt ggtgaacgac gtgtccgagc gcgacttcca gctcgagcgc
     9001 ggcagccagt gggaccgcgg caagggctgc gacaccttcg gcccggtggg cccgtggctg
     9061 gtgacgaccg acgagatcac caacccgcaa tcgctcgaga tgtggctcga cgtcaacggg
     9121 cgcaggcggc agcgcggcaa cacgcgcacg atgatcttcg gctgcgcgaa cctcgtctcc
     9181 tatatcagcc ggttcatgac gctcctgccc ggcgacatca tcaccaccgg cacgccgccc
     9241 ggcgtgggcc tgggaatgaa gccgccgcag tacctcacgg tgggcgacac gatcaccctc
     9301 ggcatccagg gcctcggcca gcagaagcag aacgtggtcg cctggacgcc cggctcgggc
     9361 ttcaagtgac cgtccgtgtc gcgcacggcc cgctcacgca atgggcggtg gcctgccttc
     9421 gcgcggtggg cgtgccggag gaggacgcga agaccgtcgg cggctgcctc gtgcagacga
     9481 gcctgtgggg catcgactcg cacggggtgc tgcgcctgac ccattacctc acgcgcctgt
     9541 ccaaggggtc gatcgtcgcg gacgcgcggc cggtggtcac taggacgggc ccggtcaccg
     9601 cgcaggtgga cggcagggac ggactcggca tcgcgcatgc ggtgctcgcc atgtcggtcg
     9661 caatcgaact ggcgaaggac gcgggcatcg ggatcgtggg cgtgggcaac tcctcgcact
     9721 gcggcgccat gggcctgtac acgagggaag cggcgcggca ggggctgatc ggcatcgcga
     9781 tgacgcattc ctcctcggtc gtcgtgccgc acggcggccg gcacaagtat ttcggcacca
     9841 accccatttc cttcgccttc gcgcgccagg gcggcgagcc ggtgtgcctc gacatggcca
     9901 cgagccaggt ggcgtggaac aaggtcctca acgcgcgcat cgagggcgat acgctggagc
     9961 ccggactcgc cgtggacgaa aacggcgagc ccacgacgga cccgcacctc gcgcgggcag
    10021 gaatcgccct cggcgggccc acctacggct acaagggcta cgggctcgcg ttcatgatcg
    10081 agctgctctg cggccccctg aacgcgatga ccttcgggcc gcgcgtgaac gacatgtacg
    10141 ggcagctcga caggccgcgc cggatcggcc acttcctcat ggccctcgac ccgcagcgct
    10201 tcgcgggcgg gccgatgctc gcggcgacgg tagacgagat cgtgcgcgac ctcaagaccc
    10261 gcggcgagat cctccacccg ggcgagcccg agctcatcca cgaacgggag cggctggcga
    10321 gcgggattcc gatcgagccc gtggcctacg cggacatggt ggcgtggtcg gacaagctag
    10381 gcgtggccgc gcccgctccg gtctcgggca cctagctcgc gctgcatcag caattcgtcc
    10441 ggcgcgacct gccgctccac atgctgcccg ggcgaagcga gcgtgaagcc caggcgctcg
    10501 agcaggcgca aggagcgatg atttccgcgc ttgagcaccg cggacaggct tcgtaccccg
    10561 cagttttcca cgagctccgc gatcatggcc tcgacggcgt ggcgcgccag cccgcgcccc
    10621 cagtaggcgc tggaaagcac ataggcgatc gccgcccggc cgtcgggatg caccgtcgcc
    10681 tggacatagc cgatgagccc ggacgccgga aggcggacca cccagttgag ccagtgctcc
    10741 cggccgtccg cggacatcct cgattcgagc ctcgcgaagc gctcgcgcaa ccagtcgagc
    10801 gacggcggcg gctcgttctc gtgctcgtaa agcgccgggt cgccgaggac cgcgaacatt
    10861 tcctgcgcgt gcgccgcggt ctgcggctcg agcgtgaccg tgtccgtctc gacgacgtgc
    10921 atgcgcgccc ggatgggaat cacctccccg acagtgccca ttccgcggcc ctgcgggcgt
    10981 gaatgcccgt cgtgtcgaac aacggcacgt ccgagtcctc ctgatcgacg agaagggata
    11041 tctccgtgca gcccaggatg atcgcctggg caccctgccg cgcaagctcg gccatgattc
    11101 tcctgtactc ggcgcgcgat tccggaagga ccttgcccag gcacagctcg tcgtagatga
    11161 cgcgatgcac gatctcgcgg tcttcccact ccggaatcag cacctggagg ccgtggcgct
    11221 cgcgaaggcg ctcgcggtag aagtcctctt ccatggtgaa gcgcgtgccc agcaggccga
    11281 cggtcgcaag tccggct
//
LOCUS       DASUEV010000361         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_671229, whole genome shotgun sequence.
ACCESSION   DASUEV010000361 DASUEV010000000
VERSION     DASUEV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671229"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1184
                     /locus_tag="VFV55_11505"
     CDS             162..1184
                     /locus_tag="VFV55_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX4944972.1"
                     /translation="MLGLLLGTGAASPQSESHLRPGSDPTVLPAAVLIADEDNDRIIL
                     VDPQGRVVWTFPEPGDLAPGETFKTPDDAFYTPDGKQIIVTHEENFAVSLVDIASRRV
                     VWRYGTPGIHGHGPNQLWNPDDALVLPDGHVIVADIKNCRILLIAQGAQTAERVYGAD
                     RRPPGGCKHDPPRIFGSPNGAFPMRNGHYLITEIQGPWHSEFDLKTGTVIRSFRVAGV
                     RYPSDSNEVAPGQYITADYSKPGQLVTFDGDGKVLWRYKPTGKDSLDHPSLAEVLPNG
                     MVIANDDSNHRVIVVDPKTNRIVWQYGETGKKGREPGRLNTPDGVDLAPPYSLLMRHA
                     KTMGTP"
     gene            1286..2551
                     /locus_tag="VFV55_11510"
     CDS             1286..2551
                     /locus_tag="VFV55_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4944973.1"
                     /translation="MPKRLNRREFLTTSVLGTAAAAAGTSPLFFPAIVRAQEPIRIGQ
                     LIPFTGFLGAIGEYGKQGATLAVEELNEKGGALGRKLELITEDEANPGVAVQKARKLI
                     EKDKVVAIEGLVSSASCLAVGDEVQRAKVLLINAGANSDEIRSKRCHRYVFSTEGCNT
                     QYVSAIGNWLIKERKISRWYFLTSDYAFGHDLLRVARNLLLANKGTEVGSDLAPTGTT
                     DFSQYITKMKAAKPDLIFHNLAGADVTNFLKQYAESGMTIEVAGGVIDSAFAWPVGES
                     LRGHFPIIWWHELNNPASKAWAARYMKRWGRPPDNQAYGDYLAVHAIAQAIDKAKSTD
                     SAKLVSALEGHTLTGAMDGLKGRPLTFRAWDHQLLQPMYVVAAKDKAKWKDKWDIFEI
                     IQEVPPRSASLEDIAIPKAESQCQLEPLA"
     gene            2568..3443
                     /locus_tag="VFV55_11515"
     CDS             2568..3443
                     /locus_tag="VFV55_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4944974.1"
                     /translation="MTPAVFAEFLLNGLVLGALYVLMALGLSIIFGMIGVINFAHGAL
                     FTLGAYVAFQVQDALGFAGALVVAPFLVGLLGMLIESTLLRHLYLQDPLQGLLLTFGL
                     AMVLEQGIRLVWGLSPKAFNVPPAFAGALTLGPLTYSKYRSFILVSVILLIAGLMLFL
                     QKTPIGTIVRAGSRDPMMVRLLGISLTPVLTLVFGLGTALAAIAGVLSAPLAGVQPAM
                     GVNVGTAAFVVVTIGGLGSFWGAIISGLLVGQVVSLSIYFQPRAAEASMYVLMAVILL
                     LRPRGLMGERWEKFD"
     gene            3440..>3763
                     /locus_tag="VFV55_11520"
     CDS             3440..>3763
                     /locus_tag="VFV55_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944975.1"
                     /translation="MIGRLVRHPLAWLIAWFLLLPNLMAAVGSTASLGTEIVVFTLLA
                     IAYNLLMGYTGLVSFGHSAFFGLGGYGAGLVQVHFVKGMVVPLLAGVAAATLAGALIG
                     FLLMRK"
BASE COUNT          613 a         1324 c         1189 g          637 t
ORIGIN      
        1 tccttgctcg ccctgcgcct tctccacaac agtgagcatt cgatcgtgag ccaactgggc
       61 tacagaaatg atgtggccgc ttcattgtgg ctggtcggac cggcgcctta gcctgggaac
      121 acgagagcat gcgacacaca ggccgatacg caatcatcgt gatgctgggc ctgctcctcg
      181 gcactggcgc ggcatctccg caatcggaaa gtcatctgcg gccgggctcg gatcccaccg
      241 tccttcctgc tgccgttctc atcgccgacg aggacaacga tcgcatcatc ctggtggacc
      301 cccagggtcg cgtcgtctgg acctttcccg agcccggcga cctcgcgccg ggggaaacgt
      361 tcaagacccc ggacgatgcc ttctacaccc ctgacggcaa gcagatcatc gtcacgcacg
      421 aggagaactt cgccgtgtcg ctggttgaca tcgccagccg tcgcgtcgtc tggcgctacg
      481 gcacgccggg catccatggc cacggcccga accagctttg gaatccagac gacgcgctcg
      541 tgctccccga cggacacgtg atcgtggccg acatcaagaa ctgccgaatc ctgctcatcg
      601 cccagggtgc ccagacggcc gagcgcgtct acggagccga ccggcggcca cccggcggct
      661 gcaagcatga cccgccgcgc atcttcggca gcccaaacgg cgcgttcccg atgcgcaacg
      721 gccactacct catcactgag atccaagggc cgtggcacag cgagttcgac ctcaagacgg
      781 gaaccgtcat ccggagcttc cgagtagccg gggtacgcta tccgtccgat agcaacgagg
      841 tcgccccggg gcagtacatt accgccgact attccaagcc cggccagctc gtgacgttcg
      901 acggcgacgg caaggtgctg tggcgctaca agccgaccgg gaaggactcg ctcgaccatc
      961 catccctggc cgaggtgctg cccaacggca tggtgatcgc taacgacgat tccaaccacc
     1021 gcgtcatcgt cgtcgacccg aagaccaatc gcatcgtctg gcaatacggc gagaccggga
     1081 agaagggccg ggagcccggc cggctcaata ccccggatgg tgtcgacctc gcgccgccct
     1141 actcactgct catgcgacat gcgaagacca tggggacgcc ctagccggtt cctcctcaag
     1201 gtggaggctg agccgtctgg agacgccttg acaacgcgcc cgtgtggaca tactttttgc
     1261 ccattcttcg cctcgaggtg accagatgcc caagcgcctg aaccgccgcg agttcctcac
     1321 gaccagtgtc ctcgggactg ccgccgcggc cgccgggacg agcccgctgt tcttccccgc
     1381 catcgtgcgc gcccaggagc cgatccgcat cgggcagctc atcccattca ccgggttcct
     1441 cggcgccatc ggcgagtacg gcaagcaggg cgccaccctc gccgtagagg agctgaacga
     1501 gaagggcggc gcgctgggcc ggaagctcga gttgatcacc gaggacgagg cgaaccccgg
     1561 cgtggcggtg cagaaggcgc gcaagctgat cgagaaggac aaggtcgttg ccatcgaggg
     1621 cctcgtgtcc tccgcctcgt gcctcgcggt cggcgacgag gtccagcggg cgaaggtgct
     1681 cctgatcaac gcgggggcga actccgacga gatccgaagc aagcgctgcc accgctacgt
     1741 gttttcgacc gagggctgca acacccagta cgtgagcgcc atcggcaact ggctcatcaa
     1801 ggagcgcaag atcagccgct ggtacttcct cacctccgac tacgcgttcg gtcacgacct
     1861 cctgcgcgtg gcgcgcaatc ttctcctggc gaacaagggc accgaggtcg ggagcgacct
     1921 cgcgcccacg ggcacgacgg acttcagcca gtacatcacc aagatgaagg cggcgaagcc
     1981 ggacctcatc ttccacaacc ttgccggcgc cgacgtgacg aacttcctca agcagtacgc
     2041 ggagtcgggg atgaccatcg aggtcgcggg cggcgtcatc gactcggcct tcgcgtggcc
     2101 ggtgggcgag tcgctccgcg gccacttccc gatcatctgg tggcacgagc tgaacaatcc
     2161 ggcgagcaag gcgtgggccg cgcgctacat gaagcgctgg ggccggccgc ccgacaacca
     2221 ggcgtacggc gactatctcg cggtccacgc gatcgctcag gcgatcgaca aggcgaagtc
     2281 cacggacagc gcgaagctgg tttcggcgct cgagggtcat acgctgacgg gagcgatgga
     2341 cgggctcaag ggccggccgc tgaccttccg cgcatgggac caccagctcc tgcagccgat
     2401 gtacgtcgtg gccgcgaagg acaaggcgaa gtggaaggac aagtgggaca tcttcgagat
     2461 catccaggag gtcccgccgc ggagcgcgtc gctcgaggac atcgcgatcc cgaaggccga
     2521 gagccagtgc cagttggagc cgctagcgta agacgccgac cccgccgatg acgcccgcag
     2581 tcttcgccga gttcctgttg aacgggctcg tgctcggggc gttgtacgtg ctgatggccc
     2641 tcggcctgtc gatcatcttc ggcatgatcg gcgtcatcaa cttcgcccac ggcgcgctct
     2701 tcacgctggg ggcgtacgtc gcgttccagg tgcaggacgc gctcgggttc gcgggcgcgc
     2761 tggtcgtcgc gcccttcctc gtcggcctcc tcggcatgct catcgagtcc acgctcctgc
     2821 gccacctcta cctccaggat ccgctccagg gcctcctgct cacgttcggc ctggcgatgg
     2881 tgctcgagca ggggatccgg ctggtctggg gcctcagccc gaaggcgttc aacgtgccgc
     2941 cggccttcgc gggcgcgctc acgctcgggc cgctcaccta ctcgaagtac cggagcttca
     3001 ttctcgtctc cgtcatcctg ctcatcgccg gcctcatgct cttcctccag aagacgccga
     3061 tcggcaccat cgtgcgcgcg gggagccgcg atccgatgat ggtccgcctc ctcggcatca
     3121 gcctcacgcc cgtcctcacc ctcgtgttcg ggctcggcac cgcactggcg gcgatcgcgg
     3181 gcgtgctctc cgcgccgctg gcgggcgtcc agccggcgat gggcgtgaac gtcggcacgg
     3241 cggccttcgt cgtcgtcacg atcggcggcc tcggcagctt ctggggcgcg atcatctccg
     3301 gcctgctcgt gggccaggtc gtgagcctca gcatctactt ccagccgcgc gcggccgagg
     3361 cttcgatgta cgtcctcatg gccgtcatcc tcctgctgcg gccgcgcggg ctcatgggcg
     3421 agcgctggga aaagttcgat tgatcggccg cctcgtccgc cacccgctcg cctggctcat
     3481 cgcctggttc ctcctcctgc ccaacctcat ggccgcggtc gggtccaccg cctcgctcgg
     3541 gaccgagatc gtggtcttca cgctgctcgc catcgcctac aacctcctga tgggctacac
     3601 ggggctcgtc tcgttcggtc atagcgcctt cttcggcctc ggcggctacg gcgccggcct
     3661 ggtgcaggtc catttcgtga agggcatggt ggtgccgctc ctcgccggcg tcgccgccgc
     3721 cacgctggcg ggcgccctca tcgggttcct gctgatgcgc aag
//
LOCUS       DASUEV010000362         6431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_547156, whole genome shotgun sequence.
ACCESSION   DASUEV010000362 DASUEV010000000
VERSION     DASUEV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547156"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..979
                     /gene="pbpG"
                     /locus_tag="VFV55_11525"
     CDS             77..979
                     /gene="pbpG"
                     /locus_tag="VFV55_11525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX4944976.1"
                     /translation="MKRVAALFALLALATASVAVDKPASRAKAMKASRTVADFDKDGD
                     PNLMSTAALVLDAKTGETLFAKNADVVHPIASITKLMTAMVVLDAKLPLDEAIQISTE
                     DLDLLKGTRSRLPVGANFRRDDLIRIALVASDNRAAAALGRSYPGGILAFVDAMNAKA
                     KLLGLEATHFVDSSGLAPSNVSSPADLARLVAQASRYDIIREYSTTPALDVTLPDSGR
                     KVNYVNTNALVRAGNWDIGLSKTGYISESGKCLVMQAMIANTPIVIVLLDSWGRLTRI
                     GDANRIKRWLEKNPAKLAGTFRAG"
     gene            1217..2512
                     /gene="urtA"
                     /locus_tag="VFV55_11530"
     CDS             1217..2512
                     /gene="urtA"
                     /locus_tag="VFV55_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX4944977.1"
                     /translation="MKRRSFIKAVTLAAAIATAGLCSTDLLAQAKTVRVGVLHSLSGT
                     MAISETVLKDVALMAFEEINAAGGVLGAKIEADVVDPASNWPLFAEKARQLITQDKVA
                     VVFGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYL
                     MSKDGGGARRFVLLGTDYVYPRTTNKILRAFLKSKGVAEADVMEEYTPFGHADYQTII
                     AKIKKFASEGKKTAVVSTINGDSNVPFYKELGNQGLKATDVPVVAFSVGEEELRGVDT
                     KPLVGHLAAWNYFMSLKNPTNDGFTKKWAAYAKAKNLPGHKDKPLTNDPMEATYIGVY
                     MWKQAVEKAKSFDTDKVVAAMAGQTFKAPSGFTASMDAKNHHLHKPVFIGEVKADGQF
                     NVVWKTPGPVKAQPWSPFIAGNDKKKDEPEVARKAVASK"
     gene            2582..4189
                     /gene="urtB"
                     /locus_tag="VFV55_11535"
     CDS             2582..4189
                     /gene="urtB"
                     /locus_tag="VFV55_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX4944978.1"
                     /translation="MKTLRAWAVVLSLLLAAAFPARASLDPVALAKLATGDGDEKVAA
                     IAALVATGDARAVVVLQALADGEMQVASGRVLLVKGEEGIDAATGEKVDPLPAAREDV
                     VANNRLRREIESALAALKIFSPDRDARLAGARELAGGANEAMLPLVKKAIAAESDPVI
                     KPLLEMTAAGLEIGSADKATRIAAIRTLGSSGDPNARMLLAPLAQKGKDGAFAERDDE
                     VRIAAQRSLSAVEGRLAWGQRAGLLFSGASLGSILLLAALGLAITYGLMGVINMAHGE
                     LIMIGAYTTYVVQNLFRASLPGAFDWYLLAAVPAAFAVSALVGMALERTVIRFLYGRP
                     LETLLATWGLSLVLIQAMRSLFGAQNVQVENPAFMSGGIEVLSGIVLPYSRIVIIAFA
                     AVVLAGMGLLLARTRLGLFVRAVTQNRSMAACVGVRTARIDTLAFGLGSGLAGLAGCA
                     LSQIGNVGPDLGQAYIVDCFMVVVLGGVGQLAGTVYAALGLGLLNKLLESWSGAVLAK
                     IAILVFIILFIQKRPQGLFALKGRVVD"
     gene            4189..5271
                     /gene="urtC"
                     /locus_tag="VFV55_11540"
     CDS             4189..5271
                     /gene="urtC"
                     /locus_tag="VFV55_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX4944979.1"
                     /translation="MLLARLYGPRGWAALTAGAAVVFVLFPALNRLVPEGSAFHVTAF
                     GVTLAGKIMCYMIVAVAMDLVWGYAGILSLGHGLFFALGGYAMGMYLMRQIGRDGVYK
                     ADLPDFMVFLDWKTLPWHWTNSDSFLWMLFLVVAVPGLLAFVFGWFAFRSRIKGVYFS
                     IITQALTYAAMLLFFRNETGFGGNNGFTDFKRILGIPIATPEARMALFMATGAALFAT
                     LLLGRFLVTSRFGRVLAAIRDAEARVMFCGYDTLWYKLFVWTLSAVLCGIAGALYVPQ
                     VGIINPGEMSPANSIEIAIWVAVGGRGTLVGPIVGAGIVNGAKSIFTVAFPEYWLYFL
                     GLIFILVTLFLPQGVVGLLSRRRKPS"
     gene            5394..6152
                     /gene="urtD"
                     /locus_tag="VFV55_11545"
     CDS             5394..6152
                     /gene="urtD"
                     /locus_tag="VFV55_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX4944980.1"
                     /translation="MGSLDLSHGVVLYLEDISVSFDGFKALDKLALTIDAGELRCVIG
                     PNGAGKTTMMDVITGKTRPDTGRAFFGQTIDLTAHTEAQIAHAGIGRKFQKPTIFESH
                     SVFENLELAMKADKRVRRTLRVKLSSAQEDRIAETMRQIGLADSARRPAGLLSHGQKQ
                     WLEIGMLLMQEPQLLLLDEPVAGMSDDETERTAELFLSLAGTHSLVVVEHDMAFIARI
                     ARKVTVLHEGSVLAEGPLDTVQNDPRVVEVYLGR"
     gene            6142..>6431
                     /locus_tag="VFV55_11550"
     CDS             6142..>6431
                     /locus_tag="VFV55_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX4944981.1"
                     /translation="MVVEGLNQYYGGSHILRDVSFEVPAGQVTTLLGRNGVGKSTLLK
                     CIAGLLPVRSGTVAFDGVDVTKLPPHARARLGLGYVPQGREIFPRLTVEENL"
BASE COUNT         1036 a         2225 c         2105 g         1065 t
ORIGIN      
        1 cttgatgcat cgcatattgt cacggacggg atatctttgc tatggttcgt cataccctga
       61 caaaccttgg gtccccatga aacgcgtcgc cgcccttttc gccctgctcg cccttgccac
      121 cgcctccgtc gcggtcgaca agcctgcctc acgtgcgaag gcgatgaagg cctcgcgaac
      181 cgtcgcggat ttcgacaagg acggcgatcc caacctgatg tccaccgccg cgctggtgct
      241 cgatgcgaag accggcgaga cgctcttcgc caagaacgcc gacgtggtcc atcccatcgc
      301 ctcgatcacc aagctgatga cggcgatggt ggtcctggac gcgaagctgc ccctcgacga
      361 ggccatccag atctccacgg aggacctcga cctgctcaag ggcacgaggt cgcgccttcc
      421 cgtgggcgcg aacttccgcc gcgacgacct catccgcatc gccctcgtgg cctcggacaa
      481 tcgcgccgcg gccgccctgg ggcgttccta tcccggcggc atactcgcct tcgtggacgc
      541 catgaacgcc aaggcgaagc tgctcgggct ggaagccacg cacttcgtgg attcgagcgg
      601 cctcgcgccg agcaatgtct cgagccccgc ggacctggcg cggctggtgg cgcaggcttc
      661 ccggtacgac atcatccgcg agtactcgac caccccggcc ctcgacgtga cgctgcccga
      721 ttccggccgc aaggtgaact acgtgaacac caacgcgctg gtgcgcgccg gcaactggga
      781 cataggcctg tccaagaccg gttacatcag cgagtccggc aagtgcctcg tcatgcaggc
      841 catgatcgcc aacaccccga tcgtgatcgt gctcctcgat tcctggggcc ggctcacgcg
      901 catcggcgac gccaaccgca tcaagcgctg gctcgaaaag aaccccgcca agctcgccgg
      961 taccttccgc gccggctgac gcatcgcggg gaaagtcccg aaacggtgcg ctgcccgatg
     1021 gcgcgtgcca tcgtggtgca agccgttcat tcccgatccc gctttcgtcg cggcccgcag
     1081 gactttctcc ttcccgatca agcccctgtg acatgaaggg cgcattggca cggtcgttgc
     1141 gctaggtgtt cccgaggcgg cgcagatgcc gccggaccac cccaaccacc ggcacctcca
     1201 tcagggaagg aaccccatga agcgacgctc attcatcaag gcggtgacgc tcgccgccgc
     1261 cattgcgacc gcgggcctct gctccaccga cctcctcgca caggccaaga ccgtcagggt
     1321 cggcgtgctg cactcgcttt ccggcacgat ggccatcagc gagaccgtgc tcaaggacgt
     1381 ggcactcatg gcgttcgagg aaatcaacgc cgcgggaggc gtgctcggcg cgaagatcga
     1441 ggcggacgtc gtcgatcccg cctccaactg gccgctcttc gccgagaagg cgcgccagct
     1501 catcacgcag gacaaggtgg cggtggtgtt cggctgctgg acctcggtat cgaggaagag
     1561 cgtgctgccg gtgttcgagg agctgaacgg cctgctcttc tacccggtgc agtacgaggg
     1621 cgaggaactg tcgaagaacg tgttctacac cggcgccgcg cccaaccagc aggctattcc
     1681 cgcggtcgag tacctgatga gcaaggacgg cggcggcgcc aggcgcttcg tgctgctcgg
     1741 caccgactac gtctatccgc gcacgaccaa caagatcctg cgcgcgttcc tgaagagcaa
     1801 gggcgtggcc gaggccgacg tcatggagga gtacacgccc ttcggccacg cggactacca
     1861 gaccatcatc gcgaagatca agaagttcgc ctccgagggc aagaagacgg ccgtggtctc
     1921 gaccatcaac ggcgattcga acgtgccctt ctacaaggaa ctgggcaacc agggcctcaa
     1981 ggcgaccgac gtgccggtcg tcgccttctc cgtgggcgag gaagagttgc gcggcgtcga
     2041 caccaagccc ctggtcggcc acctcgcggc gtggaactac ttcatgtcgc tcaagaaccc
     2101 gaccaatgac gggttcacga agaagtgggc ggcctacgcc aaggcgaaga acctgccggg
     2161 gcacaaggac aagccgctca ccaacgaccc gatggaggcg acctacatcg gcgtgtacat
     2221 gtggaagcag gccgtcgaga aggcgaagag cttcgacacc gacaaggtgg tcgccgccat
     2281 ggccggccag acgttcaagg cgccttccgg cttcaccgcc agcatggacg cgaagaacca
     2341 ccacctgcac aagccggtgt tcatcggcga ggtgaaggcg gacggccagt tcaacgtcgt
     2401 gtggaagacc cccggcccgg tgaaggccca gccctggagc ccgttcatcg ccggcaacga
     2461 caagaagaag gacgagcccg aggtcgccag gaaggccgtg gcttccaagt agcagcgcct
     2521 cgcgcggacg gcggcgggat cgttgggtcc cgctgccgca tcgcgcatcc ggagagagaa
     2581 agtgaaaacc ctgcgtgcgt gggcggtcgt gctcagcctg ctgctagccg cggcattccc
     2641 ggctcgcgct tcgctcgatc ccgtagccct ggcgaagctc gccaccggcg acggcgacga
     2701 gaaagtcgcc gccatcgctg ccctcgtggc cacgggcgac gcgcgcgccg tcgtcgtgct
     2761 gcaggcgctc gccgacgggg agatgcaggt cgcttccggc cgcgtcctgc tcgtgaaggg
     2821 cgaggaaggc atcgacgcgg cgacgggcga gaaggtcgat cccctgcccg cggcgcgcga
     2881 ggacgtcgtc gccaacaacc gcctgcgccg cgagatcgaa tcggcgctcg cggccctcaa
     2941 gatcttctcc cccgatcgcg acgcgcggct cgcgggagcc cgcgaactgg caggcggggc
     3001 gaacgaggcc atgctgcccc tcgtgaagaa ggcgatcgcc gccgaatccg accccgtgat
     3061 caagccgttg ctcgaaatga ccgccgcagg actggaaatc ggcagcgccg acaaggcgac
     3121 ccgaatcgcc gcgatcagga cgctcggatc ctcgggcgac ccgaacgcgc gcatgcttct
     3181 cgcgccgctg gcgcagaagg gcaaggacgg cgcgttcgcc gaaagggacg acgaagtgcg
     3241 catcgccgcg cagaggagcc tctcggccgt cgagggacgc ctcgcatggg gccagcgcgc
     3301 gggcctgctc ttttccggcg cgagcctcgg ctcgatcctg ctgctcgccg cgctggggct
     3361 ggccatcacc tatggcctca tgggcgtcat caacatggcc catggcgagc tcatcatgat
     3421 cggggcgtac acgacctacg tcgtgcagaa cctcttccgc gcgagccttc ccggagcctt
     3481 cgactggtac ctgctcgcgg cggtgccggc ggccttcgcc gtgtccgccc tcgtggggat
     3541 ggcgctcgag cggacggtga tccgcttcct gtatggccgc ccgctcgaga cgctgctcgc
     3601 gacctggggc ctgtcgctcg tgctcatcca ggccatgcgc agcctcttcg gcgcgcagaa
     3661 cgtgcaggtg gagaacccgg cgttcatgtc gggcggcatc gaggtgcttt ccggaatcgt
     3721 gcttccgtac agccgcatcg tgatcatcgc gttcgcggcc gttgtgcttg ccggcatggg
     3781 gctgctcctc gcgcgcacgc gcctggggct cttcgtgcgg gcggtgacgc agaaccggtc
     3841 gatggccgcc tgcgtggggg tgcgcacggc acgcatcgac acgctggcct tcggcctggg
     3901 ctccggcctc gccgggctcg cgggctgcgc cctgtcgcag atcggcaacg tgggcccgga
     3961 tctcgggcag gcctacatcg tcgattgctt catggttgtc gtgctgggcg gcgtcggcca
     4021 gcttgcgggc acggtctacg cggcgctcgg gctgggcctg ctcaacaagc tcctcgaatc
     4081 gtggtccggc gcggtgctcg ccaagatcgc gatcctcgtc ttcatcatcc tcttcatcca
     4141 gaagcgcccg cagggcctct tcgccctcaa gggccgggtg gtcgactgat gctgctcgcg
     4201 cgcctctacg gaccgagggg ctgggcggcg ctcaccgcag gggcggctgt cgtgttcgtg
     4261 ctcttcccgg cgctcaatcg cctcgtgccc gagggcagtg ccttccacgt cacggccttc
     4321 ggcgtcacgc tggccggcaa gatcatgtgc tacatgatcg tcgcggtggc gatggacctc
     4381 gtgtggggct atgcggggat cctttcgctc ggccacgggc tcttcttcgc cctcggcggc
     4441 tacgcgatgg gcatgtacct catgcgccag atcggccgcg acggcgtgta caaggcggat
     4501 cttcccgact tcatggtctt cctcgactgg aagacgctgc cctggcactg gaccaactcc
     4561 gacagcttcc tgtggatgct cttcctcgtg gtcgcagtgc cggggctcct cgcgttcgtg
     4621 ttcgggtggt tcgccttccg ctcgcgcatc aagggcgtct acttctcgat catcacgcag
     4681 gcgctcacct acgccgccat gctgctcttc ttccgcaacg agacgggctt cggcggcaac
     4741 aacggcttca ccgacttcaa gcgcatcctc ggcatcccga tcgccacccc cgaggcgcgg
     4801 atggccctct tcatggcgac cggcgcggcg ctcttcgcca cgctcctcct ggggcgcttc
     4861 ctcgtcacgt cgcgcttcgg ccgcgtgctc gccgccatcc gcgacgccga ggcgcgcgtg
     4921 atgttctgcg gctacgacac gctctggtac aagctcttcg tgtggacgct ctccgccgtg
     4981 ctgtgcggaa tcgccggcgc gctctacgtg ccgcaggtgg gcatcatcaa ccccggcgag
     5041 atgtcgcccg cgaattcgat cgagatcgcg atctgggtgg cggtgggcgg gcgcggaacg
     5101 ctcgtgggtc ccatcgtggg tgcgggaatc gtgaacgggg caaagagcat cttcaccgtc
     5161 gccttcccgg agtactggct gtacttcctc gggctcatct tcatcctggt gacgctgttc
     5221 ctgccgcagg gcgtcgtcgg gctgctttcg cggcggagga agccgtcatg agcgcccccg
     5281 ccgaactgct gcccgatccg ggcagccggc cgtggccgtg gtggccgccg gcgcagttgc
     5341 cgtccgcgca agcgaccgcg tcgggcggcc cgagctacgg ccgccatgtc gaaatgggca
     5401 gtctggacct ctcgcacggc gtcgtcctct acctcgagga catcagcgtc tcgttcgacg
     5461 gcttcaaggc cctggacaag ctcgcgctca ccatcgacgc gggcgagttg cgctgcgtga
     5521 tcggccccaa cggcgcgggc aagaccacga tgatggacgt gatcaccggc aagacccgcc
     5581 cggacacggg gcgcgccttc ttcggccaga ccatcgacct cacggcccac accgaggcgc
     5641 agatcgccca tgccggcatc ggccgcaagt tccagaagcc gacgatcttc gaaagccaca
     5701 gcgtgttcga gaacctcgag ctcgcgatga aggctgacaa gcgcgtgcgc cgcacgctgc
     5761 gcgtgaagct ttcctccgcg caggaggacc gcatcgccga gacgatgcgc cagatcggtc
     5821 tcgccgacag cgcgaggcgg ccggcgggcc tgctctcgca cggccagaag cagtggctcg
     5881 agatcggcat gctcctcatg caggagccgc aactgctcct gctggacgag cccgtggcgg
     5941 gcatgagcga cgacgagacc gagcgcaccg ccgagctctt cctctcgctc gcgggcaccc
     6001 attcgctcgt cgtcgtggag cacgacatgg cattcatcgc caggatcgcc cggaaggtga
     6061 ccgtgctgca cgagggcagc gtgctcgcgg agggccctct cgacacggtg cagaacgacc
     6121 ctcgcgtcgt ggaggtgtac cttggtcgtt gaggggctga accagtatta cggcggcagc
     6181 catatcttgc gcgacgtgag cttcgaggtg ccggccggac aagtcacgac gctcctcggc
     6241 cgcaacggcg tcggcaagtc gacgctgctc aagtgcatcg cgggcctctt gccggtgcgc
     6301 tcggggaccg tagctttcga cggcgtcgac gtgacgaagc tgccgccgca tgcgcgcgcg
     6361 cggctgggcc tgggctacgt gccccagggc cgcgagatct tcccccggtt gaccgtcgag
     6421 gagaacctga g
//
LOCUS       DASUEV010000363         6447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_1713353, whole genome shotgun sequence.
ACCESSION   DASUEV010000363 DASUEV010000000
VERSION     DASUEV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713353"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VFV55_11555"
     CDS             <1..1780
                     /locus_tag="VFV55_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX4944982.1"
                     /translation="NLLPQVGEDGAVAAAFVLISDITRHRSSEQAVRESEERLRKFSD
                     ATHEGIVFHDKGVITDCNDAALRLIGYRYQELVGKPVLDFVAPESRDTVLNNIRLGFE
                     RPYEGSIVHKGGERIAVEITGKVMPVKDKHYRMTVIRDIRDRKEAEARIQFLAHHDTL
                     TGLPNRVLLMDRLEFILASSRRRKARVGILFVDLDNFKTVNDSLGHAAGDTLLRQVAT
                     RIEGTLRSVDVVSRLGGDEFLVVLPDLEDEHSPIAVAEKLLAAVSEPVVLEGQSLSVS
                     PSIGIAIFPRDGANADTLIRNADAAMYLAKDRGRSNFQFFSEGLSQTASNTLSLETRL
                     REAIREEGFVIHYQPQVRVDTGRLVGLEALIRWPQRDGTTVMPNDFIPVAERRGLIMP
                     IGTWVLRNACLQNRQWQLAGLPHVPVAVNLSGIQFKQRNLVAEVERVLAETGLNAAWL
                     EFELTESMLMEETPELMRTLDGLHELGVKVAIDDFGMGHTSLSHLKRFPIDRLKIDRS
                     FVRDIAGDPDDRAITAAIIDLARNMGITSIAEGVESRAQLDFLAGRGCDEMQGFLTSP
                     PLPAAEAAAFLAASRIEPGTLPVILS"
     gene            1872..3344
                     /locus_tag="VFV55_11560"
     CDS             1872..3344
                     /locus_tag="VFV55_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944983.1"
                     /translation="MPEPDAEEKKKPAEEAPPADRLAVTHHRAHLAGRDLDYTVTCGT
                     IVLKEQSEKDGKAESDKARASVFFVAYTLDGAEPESRPVTFSFNGGPGSSSVWLHLGL
                     LGPRRVALDDEGRAPPPPGRLVDNEHSLLDRSDLVFIDPVGTGYSRMVAGEKVAEYHD
                     YQRDLESVGEFIRLYCSRYARWSSPKYLIGESYGTTRAAGLAGHLLERHGMYLNGLML
                     ISCALDFTVLRFETNNDLPPVLFLPTFASTAWYHRKLPADLQAKPLRAVLDEVEAFAG
                     GEYAAALFRGAKLSGAERSGIAAKVARYTGLAQAYVERTGLRVEIFRYCKELLRAEGR
                     TVGRLDSRFTGVDRDSAGERFEFDPGFAQIYGAYAAAMNDYVRRALRFEADAPYEVLK
                     GLYLNWGWKDFANRYASVGETLRKALAMNPHMRVLVANGYYDFATPYFASDYTFDHLG
                     VDAKLRDNFTVAYYEAGHMMYVHKPSLERLAADLRAFVAP"
     gene            complement(3731..4795)
                     /gene="queG"
                     /locus_tag="VFV55_11565"
     CDS             complement(3731..4795)
                     /gene="queG"
                     /locus_tag="VFV55_11565"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX4944984.1"
                     /translation="MDTGLSPAALSERVKRWGREAGFDAVAVSGIDLAEEEARLLDWL
                     GRGWHGAMDYMARHGTRRSRPAELLPGTASVVTVRLDYQPGEARDAEEVLADPSLAFV
                     SRYAVGRDYHKVLRARLQALAERMTAELGEFRYRAFTDSAPVLEVALAARAGLGWRGK
                     HTLLLTRDAGSYYFLGELYVGLDLAKDAPVTEHCGTCERCLDACPTKAIVAPYQLDAR
                     RCISYLTIENPGPIPEEFRAAMGNRIYGCDDCQLACPWNKYARKATVPDFAARNGLDA
                     ATLTELFAWTRDEFDSRLEGSAIRRIGHARWLRNIAVALGNAPTSPAVQQALRARADD
                     PEPLVREHVAWALQRHEARR"
     gene            4832..5290
                     /gene="tsaE"
                     /locus_tag="VFV55_11570"
     CDS             4832..5290
                     /gene="tsaE"
                     /locus_tag="VFV55_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX4944985.1"
                     /translation="MPTYPLPDENATLALGAALAPHMAPGLVLALRGELGAGKTTFVR
                     GLLRALGYAGRVKSPTYTLVEVYEVSRLNFYHFDFYRFKDPSEWIDAGFRDLFNGRGV
                     CLIEWPERARGQLPPADLDILLEWAEPGRRATLASASPAGERLLAALRPA"
     gene            5206..>6447
                     /locus_tag="VFV55_11575"
     CDS             5206..>6447
                     /locus_tag="VFV55_11575"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX4944986.1"
                     /translation="MGGTGAQGDACFRFARGRALAGRLAAGLILAFAAPAAFAAQAIL
                     ASRVWPAEEYTRVTIETAKPVRHQFFAVQDPERLVLDLEGVDADEELKSLASKVRPDD
                     PYIRQVRVAMNRPNVARVVFDLKSAVKPDVFPLAPAGEYRHRLVLDIYPEKPRDPLLA
                     LVQPGPDPIGEMARAPVMESPPDLPVVKLEPGKVAEPAPAEKAKPPPKMMRMVTIVID
                     AGHGGEDPGARGRGGTLEKDVTLAIARRLKERIDLEPGMRALLTRDGDYFIGLAQRVH
                     KARRVQADLFVSVHADAFVQAHARGSSVFALSERGATSAAARLLAKRENESDLIGGVN
                     LGVRDPVLARTLLDLSQTATINDSLKLGRAVLGELGDINSLHKSAVEQAGFAVLKAPD
                     IPSILVETAFISNPQEEGRLKD"
BASE COUNT         1030 a         2246 c         2203 g          968 t
ORIGIN      
        1 gaacctcctg ccgcaggtcg gcgaggacgg cgcggtggcg gcggccttcg tgctcatctc
       61 ggacatcacg cgccaccggt cctccgaaca ggcggtgcgc gaaagcgagg agcggctgcg
      121 caagttctcg gacgccaccc acgagggcat cgtcttccac gacaagggcg tgatcacgga
      181 ctgcaacgac gccgcactgc gcctcatcgg gtaccgctac caggaactcg tgggcaagcc
      241 ggtgctcgat ttcgtcgccc ccgagtcgcg cgacacggtc ctcaacaaca tccgcctggg
      301 tttcgagcgc ccctacgagg ggtcgatcgt gcacaaaggt ggcgagcgca tcgccgtgga
      361 gatcaccggc aaggtgatgc ccgtcaagga caagcactac cgcatgacgg tgatccgcga
      421 catccgcgac cgcaaggagg ccgaggcgcg catccagttc cttgcccacc acgacacgct
      481 taccgggctg cccaaccgcg tgctcctcat ggaccgcctg gagttcatcc tcgcctcgtc
      541 ccggcggcgc aaggcgaggg tgggcatcct gttcgtcgac ctggacaact tcaagacggt
      601 gaacgattcc ctcggccacg ccgcgggcga cacgctgctt cggcaggtcg ccacccgcat
      661 cgagggcacg ctgcgctcgg tcgacgtggt ctcgcgcctg ggcggcgacg agttcctggt
      721 ggtcctgccc gatctcgagg acgagcactc gccgatcgcg gtggcggaga aactgctcgc
      781 ggcggtgagc gagccggtcg tcctcgaggg gcagagcctt tcggtgagcc cgtccatcgg
      841 catcgccatc ttcccgcgcg acggcgccaa tgcggacacg ctcatccgga acgccgacgc
      901 cgcgatgtac ctcgccaagg accgtggccg cagcaacttc cagttcttca gcgaggggct
      961 ttcgcagact gcgtcgaaca cgctctcgct cgagacgcgc ctgcgcgaag ccatccgcga
     1021 ggaaggcttc gtgatccact accagccgca ggtgcgcgtc gacaccggcc gcctggtcgg
     1081 cctcgaggcg ctcattcgct ggccgcaacg cgacggcacg acggtgatgc ccaacgactt
     1141 catcccggtg gccgagcggc gcgggctcat catgcccatc ggcacgtggg tgctgcgcaa
     1201 tgcctgcctc cagaaccgcc agtggcagct cgccgggctg ccgcacgtgc cggtggcggt
     1261 caacctctcg ggcatccagt tcaagcagag gaacctcgtg gccgaggtgg aacgggtcct
     1321 tgccgagacc ggcctgaacg cggcgtggct cgagttcgag ctgaccgaga gcatgctgat
     1381 ggaagagacg ccggaactca tgcgcacgct cgacgggctg cacgaactgg gcgtgaaagt
     1441 cgccatcgac gacttcggca tggggcacac gtcgctgtcg cacctgaagc gcttccccat
     1501 cgacaggctc aagatcgacc gcagcttcgt gcgcgacatc gcgggcgatc cggacgatcg
     1561 cgccatcacg gcggccatca tcgacctcgc acgcaacatg ggcatcacct cgattgccga
     1621 gggcgtggaa agccgcgcgc aactcgattt cctcgccggg cgcggctgcg acgagatgca
     1681 gggcttcctc accagcccgc cgctgccggc ggccgaggcg gcggccttcc tggccgcaag
     1741 ccgcatcgag cccggcacgc tgcccgtcat cctgtcctga gcggcctggt cgcgccggcg
     1801 cggcgggcca tcgcgcgggc cggccacgct tcggatacga tggccgcgcc attccatgag
     1861 aggggacacc gatgcccgaa cccgacgccg aggaaaagaa gaagcccgcc gaggaggcgc
     1921 cgccggccga ccggctggcc gtcacgcacc atcgcgcgca cctggccggc cgtgacctcg
     1981 actacacagt cacctgcgga acgatcgtcc tgaaggagca gtccgagaag gacggcaagg
     2041 ccgagagcga caaggcgcgg gccagcgtct tcttcgtggc gtacacgctg gacggcgccg
     2101 agcccgagtc gcgcccggtg acgttctcgt tcaacggcgg gccggggtcg agttcggtct
     2161 ggctgcacct cgggctcctg ggaccgcggc gcgtggcact cgacgacgag ggccgcgccc
     2221 cgccgccgcc gggccggctc gtcgacaacg agcactcgct gctcgatcgt tccgacctcg
     2281 tgttcatcga cccggtgggc acgggctaca gccgcatggt ggcgggcgag aaggtcgccg
     2341 agtaccacga ctaccagcgc gacctggaga gcgtgggcga attcatccgc ctctattgct
     2401 cgcgctacgc gcgctggtcg agccccaagt acctcatcgg cgagtcctac ggcaccacgc
     2461 gcgccgccgg gcttgcgggg cacctgctgg agcgccacgg aatgtacctg aacgggctca
     2521 tgctgatctc ctgcgcgctc gacttcacgg tgctgcgctt cgagaccaac aacgacctgc
     2581 cgccggtcct cttcctgccc accttcgcgt cgaccgcttg gtaccaccgc aagcttcccg
     2641 cggacctgca ggcgaagccg ctgcgcgcgg tgctcgacga ggtcgaggcg ttcgcgggcg
     2701 gcgagtatgc cgcggcgctc ttcaggggcg cgaagctttc cggcgccgag cgctcgggca
     2761 tcgccgcgaa ggtcgcccgc tacaccgggc tcgcgcaggc atacgtcgaa cgcaccggcc
     2821 tgcgtgtcga gatcttccgc tactgcaagg agctcctgcg cgccgagggc cggaccgtgg
     2881 gacgtctcga cagccgcttc accggcgtcg atcgcgattc cgccggcgag cgcttcgagt
     2941 tcgaccccgg cttcgcgcag atctacgggg cctacgccgc cgcgatgaac gactacgtgc
     3001 gccgcgcgct caggttcgag gccgacgcgc cctacgaggt gctcaagggg ctctacctca
     3061 actgggggtg gaaggacttc gccaaccgct acgcctcggt cggcgagacg ctgcgcaagg
     3121 ccctcgcgat gaacccgcac atgcgcgtgc tggtggccaa cggctactac gatttcgcca
     3181 cgccgtactt cgccagcgac tacacgttcg accacctcgg cgtggacgcg aagctgcgcg
     3241 acaacttcac cgtcgcctac tacgaggcgg ggcacatgat gtacgtgcac aagccctcgc
     3301 tcgagcgcct ggccgccgac ctgcgggcct tcgtcgcgcc gtagccgggg ccgtcacgcg
     3361 catcgaagcg tccgcttttc gtcccaagtg cacggtgtcc gcacgaggta accttcactc
     3421 gagtgaggta gcgcgccggg tctgcacggc cttcaacgga gcccgacgca tggggtcagg
     3481 tcggttggat ttttcttggt cttgggccag atcaacttgc cgatagctgc gacttcggcg
     3541 gcatctcgac ataggttcga gccgtgacgc ccgaagcata ttgacctggc ccaagaccaa
     3601 aaaagaatcc aaccgacctg accccgattc agccttccgt ttcgacagcg gtgataactg
     3661 cgttctaacg cgaaggcctc ctcaagcgtc ggccaccgtg aagtggggac ggaacgcacc
     3721 gtagcgccga tcagcgacgg gcttcgtggc gctgcagggc ccaggcgacg tgctcgcgca
     3781 cgaggggctc cgggtcgtcg gcgcgggcgc gcagggcttg ctgcacggcg ggagaggtgg
     3841 gcgcgttgcc cagggccacg gcgatgttgc gcagccagcg cgcgtggccg atgcggcgga
     3901 tcgcggagcc ctcgagccgg gaatcgaatt cgtcgcgcgt ccaggcgaag agctccgtca
     3961 gcgttgccgc gtcgagcccg tttcgcgccg cgaagtccgg caccgtggct ttgcgcgcgt
     4021 acttgttcca cgggcaggcg agctggcagt cgtcgcagcc gtagatgcgg ttgcccatgg
     4081 cggcgcggaa ctcctccggg atggggccgg ggttctcgat ggtgaggtag gaaatgcagc
     4141 ggcgcgcgtc gagctggtaa ggggccacga tcgccttcgt cgggcaggcg tcgaggcagc
     4201 gctcgcaggt gccgcagtgc tccgtcacgg gcgcgtcctt cgccaggtcg agtcccacgt
     4261 agagctcgcc gagaaagtag tacgagcccg cgtcgcgcgt gagaaggagc gtgtgcttgc
     4321 cgcgccagcc gagcccggcg cgcgccgcga gggccacctc cagcaccggg gcgctgtcgg
     4381 tgaaggcgcg gtagcggaac tcgcccagct ccgcggtcat gcgctcggcg agcgcctgca
     4441 gccgtgcgcg cagcaccttg tgatagtcgc ggccgacggc gtaacgggag acgaaggcga
     4501 gcgagggatc ggcgaggact tcttccgcgt cccgcgcctc gccgggctgg tagtccagcc
     4561 gcacggtcac cacgctcgcg gtgccgggca gcagttccgc gggccgcgaa cggcgcgtgc
     4621 cgtggcgtgc catataatcc atcgcaccgt gccacccgcg gccgagccag tcgaggaggc
     4681 gcgcttcctc ctcggcgagg tcgatgcccg acaccgcgac ggcgtcgaac ccggcctcgc
     4741 ggccccagcg cttgacgcgt tcggacaagg cggcggggga gagtcccgta tccatcgcgc
     4801 cgatggtagc ccgaagcgcc ccttcatccc cgtgcccacc tacccgctcc ccgacgaaaa
     4861 cgcaacgctc gcgctcggcg ccgcactcgc gccgcatatg gcgcccgggc tggtgctcgc
     4921 actgcgcggc gaactgggcg cgggcaagac caccttcgtg cggggcctgt tgcgggccct
     4981 cggctacgcc ggtcgcgtga aaagccctac gtacacactg gttgaagttt atgaagtttc
     5041 gaggttaaac ttctatcact ttgattttta tcggttcaaa gacccatcgg aatggatcga
     5101 cgcgggcttt cgagacctgt tcaacgggcg cggcgtgtgc ctgatcgagt ggccggaacg
     5161 ggcacgggga caactgccgc ctgcggatct ggacatcctg ctcgaatggg cggaaccggg
     5221 gcgcagggcg acgcttgctt ccgcttcgcc cgcgggcgag cgcttgctgg ccgccttgcg
     5281 gccggcctga tcctcgcttt cgcggcaccg gccgcgttcg ccgcgcaggc catcctcgcc
     5341 tcgcgtgtct ggcccgcgga ggaatacacg cgcgtcacga tcgaaaccgc gaagccggtc
     5401 cgccaccagt tcttcgccgt gcaggatccc gagcgcctcg tgctcgacct ggaaggcgtg
     5461 gacgcggacg aggaactgaa gagcctcgcc agcaaggtgc gccccgacga tccctacatc
     5521 aggcaggtgc gcgtggcgat gaaccgcccg aacgtggccc gcgtggtctt cgacctgaag
     5581 agcgcggtga agcccgacgt cttcccgctg gcgcccgcgg gagagtaccg gcaccggctg
     5641 gtgctcgaca tctacccgga aaaaccgcgc gacccgctgc tggcgctcgt gcagcccggg
     5701 ccggacccga tcggcgagat ggcgcgggcg ccggtcatgg aatctccccc cgacctgccg
     5761 gtggtgaagc tcgaacccgg caaggtcgcc gagcccgcgc cggcggagaa ggcgaaaccg
     5821 ccgccgaaga tgatgcgcat ggtcacgatc gtcatcgacg ccgggcacgg tggcgaggac
     5881 ccgggcgcgc gcgggcgcgg cggcacgctg gagaaggacg tgacgctcgc catcgcccgg
     5941 cggctcaagg agcgcatcga cctcgagccg ggcatgcgcg cgctgctcac gcgcgacggc
     6001 gactacttca tcggcctcgc gcagcgcgtg cacaaggcgc ggcgcgtgca ggcggacctc
     6061 ttcgtctcgg tgcacgccga cgccttcgtg caggcgcacg cgcgcggctc ttccgtcttc
     6121 gcgctttcgg agcggggcgc gacctcggcc gccgcgcgcc tgctcgccaa gcgcgagaac
     6181 gagtcggacc tgatcggcgg cgtgaacctg ggcgtgcggg acccggtgct cgcaaggacg
     6241 ctgctcgacc tgtcgcagac ggcgaccatc aacgattccc tcaagctcgg cagggcggtg
     6301 ctcggcgagc tgggcgacat caactcgctg cacaagagtg cggtggagca ggccggcttc
     6361 gcggtgctga aggcccccga cattccctcg atcctggtgg agacggcgtt catctccaac
     6421 ccgcaggagg aagggcgcct caaggac
//
LOCUS       DASUEV010000364         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_202915, whole genome shotgun sequence.
ACCESSION   DASUEV010000364 DASUEV010000000
VERSION     DASUEV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202915"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFV55_11580"
     CDS             <1..487
                     /locus_tag="VFV55_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX4944987.1"
                     /translation="AMDPRLKELVRKSVLADELSDGDVEVVAALVRLHRLEDGQVISP
                     EGEPDSHMHVIVNGALAVSRPGPGGAWENLHVMTAGDLVGELSFLDGTPRFAALRALG
                     QTEIFSLDRADLESLVEKHPWIAYRLMRAIVRFTHVLQRRMSMQSVELMHYLYKSQAK
                     Y"
     gene            590..1948
                     /locus_tag="VFV55_11585"
     CDS             590..1948
                     /locus_tag="VFV55_11585"
                     /inference="COORDINATES: protein motif:HMM:NF003759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEX4944988.1"
                     /translation="MHTIKRGLDLPIAGKPVQAIEDGVAVSRVALLGADYVGLKPTMH
                     VQPGDQVLRGQLLFEDKKSPGVRHTAPAAGRVADIHRGEKRAFVSVVIEVSPDDGPQA
                     QATFANWGGAPPREGDGPALRALLLESGLWTAFRTRPFSRVPAPDAVPEAIFVTAMDS
                     RPLAPSMEVALAGREEEYAEGILALRDLTPGTVFVCRAPGSTLRAPRAERIAIEEFAG
                     PHPAGLSGTHIHFLHPAGHGRSVWHVGAQDVAAIGHLLATGKLDVARVISLAGPGIAR
                     PRLLRTRLGASLAQLAANELVPGEQRVVSGSVLDGRTAMSESVCYLGRHHQQVSALPE
                     GRARELLGWIAPGTDKFSVAGVVLGAFSRARARPFSTTTNGSKRAMVPIGTHEAVMPL
                     DILATFLLRALLSRDLERAEALGALELDEEDLALCTYADPGKNDYAPLLREALSRLEK
                     EG"
     gene            1949..3190
                     /locus_tag="VFV55_11590"
     CDS             1949..3190
                     /locus_tag="VFV55_11590"
                     /inference="COORDINATES: protein motif:HMM:NF003756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEX4944989.1"
                     /translation="MNALERLLEKAGRPFGPGKPLARFYALFEAADTFALTPPSVTAG
                     ITHVRDALDLKRMMMIVVIAMMPCILMAMYNTGLQANLAIDPAKAAALEGWRHDVMRL
                     LGAGYSPQSVGACFLHGSLYFLPLYAITMAAGGLWEVLFAIVRRHEVNEGFFVTGLIF
                     PLTLPPTMPLWEAALGISFGVVVAKEVFGGTGMNFINPALAARAFVFFAYPGDISGDQ
                     AWTAVPPGTAIDGFSGATLLGQMRQMTGPFEQQGYSWTTAFLGFEKGSLGETSALACL
                     AGAIFLIVTRVGSWHTMAGAAAGTAAMALLFNAIGSSTNPWFAVPFWWHMVLGGWAFG
                     MAFMATDPVTSPFTEKGRLVYGFLIGVFVVLIRVVNPAYPEAVMLVILFMNVMAPLID
                     YGFVKANIVRRARRGGARGET"
     gene            3177..3968
                     /locus_tag="VFV55_11595"
     CDS             3177..3968
                     /locus_tag="VFV55_11595"
                     /inference="COORDINATES: protein motif:HMM:NF003749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEX4944990.1"
                     /translation="MARLDSTRYTILFATAVCVVCAMLVSVAAVSLQPRQKANARLYM
                     EKNVLVAAGLARPGQDLGVRDVEKYFEKDIRARLVDLATGELLPEGKIDPRAYDQRAA
                     RNDPVSSRVAPANAAGIGRLPKYGVAYFVMKGDQAEQVVIALEGLGMWGTVYGFLALD
                     RDGNTLRGLTYYDQKETPGLGGEIGNPAWQALWKGRRAYDDQGIPRITVIKGQAGPPD
                     KDPLRVDGMSGATITSNAITRLMRFWLSENGYGRFLLRVRVRGFT"
BASE COUNT          584 a         1431 c         1371 g          627 t
ORIGIN      
        1 agccatggat ccgcgcctga aggaactggt ccgcaagtcc gtcctggccg acgaactttc
       61 ggatggcgac gtcgaggtcg tggccgccct cgtccggctg caccggctgg aggacggcca
      121 ggtgatcagc cccgaaggcg aacccgacag ccacatgcac gtgatcgtga acggcgcgct
      181 tgccgtgtcg cggcccggcc cgggcggcgc gtgggaaaac ctgcacgtca tgaccgcggg
      241 cgacctagtg ggcgagctct cgttcctcga cggcactccg cgcttcgccg cgcttcgcgc
      301 cctgggccag acggagatct tcagcctcga ccgcgcggac ctcgaaagcc tggtcgagaa
      361 gcacccgtgg atcgcctacc gcctgatgcg cgccatcgtg cgcttcacgc acgtcctgca
      421 gcggcgcatg agcatgcagt ccgtcgagct catgcactac ctctacaaga gccaggccaa
      481 gtactgagcc ggttgattca cgtcaatggg ccgccggccc agtcccctac tcttgcctct
      541 ggccggcggc gcgccgacgc cttgaccatc gcaagacgcc cccaggacga tgcacacgat
      601 caaacgcggt ctggatctcc ccatcgccgg caagccggtc caggcgatcg aggacggggt
      661 ggccgtgtcc cgcgtggcgc tgctgggcgc cgattacgtc ggcctgaagc ccacgatgca
      721 tgtgcagccc ggcgaccagg tgctgcgcgg gcaactgctc ttcgaggaca agaagtcgcc
      781 cggcgtgcgc catacggcac cggcggcggg acgcgtggcc gacatccatc gcggcgaaaa
      841 gcgcgccttc gtttcggtcg tgatcgaggt ttcccccgac gacggcccgc aggcccaggc
      901 cacgttcgcg aactggggcg gcgcccctcc ccgcgaggga gacgggcccg cgctgcgcgc
      961 gctgctcctg gaatcgggcc tgtggaccgc cttccggacg cgtccgttca gccgcgtgcc
     1021 cgcgccggac gcggtgccgg aagcgatctt cgtcaccgcc atggattcgc gcccgctcgc
     1081 gccgagcatg gaggttgcgc tcgccggccg cgaggaggag tacgccgagg gcatcctcgc
     1141 gctgcgcgac ctcacgcccg gaaccgtatt cgtctgccgt gccccgggct cgacgcttcg
     1201 cgcgccgcgc gccgagcgca tcgcgatcga ggaattcgcc gggccgcacc cggccgggct
     1261 ctccggcacg cacatccatt tcctgcaccc ggccggccac ggccgcagcg tctggcacgt
     1321 gggcgcgcag gacgtcgccg ccatcgggca ccttcttgcc acgggaaagc tggacgtcgc
     1381 gcgggtcatc tcgctggccg gccccggaat cgcgcgcccg cgcctgttgc gcacgcggct
     1441 gggcgcatcg ctcgcgcaac ttgccgcgaa tgaactcgtt cccggcgaac aacgcgtggt
     1501 atcgggctcg gtgctcgacg ggcgcaccgc catgagcgag tcggtgtgct atctcggccg
     1561 ccatcaccag caggtgagcg cgctgccgga agggcgcgcc cgcgagctgc tcggctggat
     1621 cgctcccggc accgacaagt tctcggtggc cggcgtcgtg ctgggcgcct tctcccgcgc
     1681 gcgcgcccgc cccttctcga cgacgaccaa cgggtcgaag cgcgccatgg tgcccatagg
     1741 cacccacgag gcggtgatgc ccctggacat tctcgccacc ttcctgctgc gcgcgctcct
     1801 ctcccgcgac ctggagcgcg ccgaggcgct gggggcgctc gaactcgacg aggaggatct
     1861 cgcgctctgc acctacgccg acccgggcaa gaacgactac gcgccgctgc tgcgcgaggc
     1921 gctgtcccgc ctcgagaagg agggctgagt gaacgccctc gagaggctgc tcgagaaggc
     1981 gggccgcccc ttcggacccg gcaagccgct ggcgcgcttc tatgcgctct tcgaggcggc
     2041 ggacaccttc gccctgacac ccccgtcggt gacggccggg atcacgcacg tgcgcgacgc
     2101 gctggacctc aagcgcatga tgatgatcgt cgtcatcgcc atgatgccgt gcatcctcat
     2161 ggcgatgtac aacaccggcc tgcaggccaa cctcgcgatc gacccggcga aggccgccgc
     2221 gctcgaaggc tggcgccacg acgtgatgcg gctgctgggc gccggctact cgccgcagag
     2281 cgtcggcgcg tgcttcctgc acggctcgct ctacttcctg ccgctctacg cgatcacgat
     2341 ggccgcgggc gggttgtggg aagtgctctt cgcgatcgtg cgccggcacg aggtgaacga
     2401 gggcttcttc gtcaccggcc tcatctttcc gctcacgctg ccgcccacga tgcctctctg
     2461 ggaagcggcg ctgggcatca gcttcggcgt ggtggtcgcc aaggaggtgt tcggcggcac
     2521 ggggatgaac ttcatcaacc cggcgctcgc ggcgcgcgcc ttcgtgttct tcgcctatcc
     2581 cggcgacatc tcgggcgacc aggcgtggac cgcggtcccg ccgggcacgg ccatcgacgg
     2641 cttctcgggc gcgacgctcc ttggacagat gcgccagatg accggaccgt tcgagcagca
     2701 ggggtactcg tggaccaccg cgttcctcgg cttcgagaag gggagcctcg gcgaaacctc
     2761 ggcgctggcc tgcctggccg gcgccatctt cctcatcgtg acccgcgtgg gctcgtggca
     2821 cacgatggcc ggcgccgcgg ccggcacggc ggccatggcc ctgctgttca acgccatcgg
     2881 ctcgtccacc aacccgtggt tcgcggtgcc gttctggtgg cacatggtcc tcggcggctg
     2941 ggcgttcggc atggcgttca tggcgaccga cccggtgacc tctcccttca cggagaaggg
     3001 ccggctggtc tacggcttcc tgatcggggt gttcgtcgtc ctgatccggg tggtgaaccc
     3061 ggcctatccc gaggcggtga tgctggtgat cctgttcatg aacgtcatgg ccccgctcat
     3121 cgattacggg ttcgtgaagg ccaacatcgt ccgccgggca cgcaggggag gcgcgcgtgg
     3181 cgagacttga ttccacccgc tacacgatcc tcttcgccac cgcggtgtgc gtcgtgtgcg
     3241 ccatgctggt gtcggtggcg gccgtctcgc tgcagccgcg gcagaaagcc aacgcgcgcc
     3301 tgtacatgga gaagaacgtc ctggtggccg cggggctcgc caggccggga caggacctgg
     3361 gcgtgcgcga cgtggagaag tacttcgaga aggacattcg cgcgcgcctg gtcgatctcg
     3421 ccaccgggga actgctgccg gagggaaaga tcgatccgcg cgcctacgac cagcgcgccg
     3481 cgcgcaacga cccggtttcg agccgcgtcg cgcccgccaa tgcggccggc atcggacggc
     3541 tgccgaagta cggcgtggcc tacttcgtca tgaagggcga ccaggccgag caggtggtga
     3601 tcgccctgga aggcctcggg atgtggggca cggtctacgg attccttgcg ctcgaccgcg
     3661 acggcaacac cttgcgcggg ctcacctact acgaccagaa ggaaacgccc ggcctgggcg
     3721 gcgagatcgg caatcccgcc tggcaggcgt tgtggaaggg ccgccgcgcc tacgacgacc
     3781 aggggatccc gcgcatcacc gtgatcaagg gtcaggcggg gcccccggac aaggacccgc
     3841 tgcgcgtcga cggcatgtcc ggcgccacca tcaccagcaa cgccatcacg cggctgatgc
     3901 gcttttggct gtccgagaac ggctacgggc gcttcctcct ccgggtcaga gtgcgaggct
     3961 tcacatgagc gcgatcaccc cgccggcggg cgccttcggc aagcgcgagc gcg
//
LOCUS       DASUEV010000365         4854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_337262, whole genome shotgun sequence.
ACCESSION   DASUEV010000365 DASUEV010000000
VERSION     DASUEV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337262"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..3951)
                     /gene="hrpA"
                     /locus_tag="VFV55_11600"
     CDS             complement(85..3951)
                     /gene="hrpA"
                     /locus_tag="VFV55_11600"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX4944991.1"
                     /translation="MTALPEDLLAATLAADHPKLAALADTVRARMAAGRACERERSLL
                     EDLLARSRNAYAGRLARLPDPRFPDDLPIAQRRDDIAALVAKHPVTIVCGETGSGKTT
                     QIPKICLALKRGAAGLIGCTQPRRIAARSLATRLAHELPGTPRGFVGHKIRFQDRTGP
                     ETVLKVMTDGILLAETHSDRELRAYDTIIVDEAHERSLNIDFLLGYVKRLVVARPELK
                     VIVTSATIDTQRFSDFFGGAPVIEVSGRTWPVEVRYRPEFFEVDDEDDEPVDMNEAIA
                     RAVDEIASEARTGDILVFLPGEREIREAAETLRKHHPKGAEILPLFSRLSAEEQDRVF
                     EPAAHRRIVLSTNVAETSLTVPGIHYVIDTGLARVKRFSPRQKIDQLRIEPVSQAAAR
                     QRAGRCGRVASGVAIRLYAEENFAARPEFTTPEILRTSLASVILRMAALELGNIAAFP
                     FIEPPTPRQIEDGYRSLFELGAIDERRVLTPLGHELARLPLDPRIGRMLVAAREFNCL
                     REMLILASALSIQDPRDRPQDKRQESDRAHEEFRDESSDFQQLINLWKFFDEAFHHKE
                     SNRKLWATCREHFLSYVRMREWRDLAGQLRELAADLKIRENTTDATYEQVHRALLTGL
                     ISNVGLKAQEGDHYNAPRGIPFAIWPGSGLKKGRPRWVMAGELQETTRVFARNVARID
                     PEWIEKAAAHLVTRTHAEPHWDRARGEVMAYESVALYGLVIAARRKVSYGRLDPARSR
                     EVFIEGALVAGEFDTKLPFFAHNRALIREVEDLEHRARRQDVLVDERAIFGFYAARLP
                     ADVRDTVSLEAWYREAGKADAKLLFLAKSDLMRHGAESVTEELFPKQLRIGNDAFALA
                     YRFEPGHPMDGVTMNVPLALLNQVDEAVIDWLVPGMVRDKVAWTMKALPKRVRTLLVP
                     VPEHVTKFLERVNPGERPIAEEVLAYASRLAGERLDADVWSREDAPAHLAMNLRVVDD
                     AKRELAMGRDLAALRRQLGEAAQLTLAQSKPGLEREGITAWDFGDLPAEVSFRRGNQA
                     LTGYPALVDEGASVAIRLFDTRDKAVQAHRGGVKRLLAFELREQVKYLERGPQGFNAL
                     ALKLQAVTSAEKLREDIAAAVIDRAFIGDDETPRTPKSFEEQKKRARTRLPAVTEALT
                     RHVIAIAEAHHALTAVLAQGASLGKVAHEVKAQRDQLVHPGFLARTPWERLEHIPRYL
                     KGGALRLQKYRANAERDLRHAPVVAGLWAQYEADREAGKGGARLEEFRWLVEELRVSL
                     FAQELKTPLPVSAKRLQKFRDEYLRR"
     gene            complement(3989..4684)
                     /locus_tag="VFV55_11605"
     CDS             complement(3989..4684)
                     /locus_tag="VFV55_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944992.1"
                     /translation="MSTEPGHPKRAPQSLSQEIEALQVSELFGNLGVSAAAAYVGTLL
                     CVAVFLDDGLRGPHIVWLAYGSLVAALRLGLAWLHHARGPALEPRLWAKLAVLGNVLA
                     GIQWGLLGTWLFPEEPGYRQTFAIMVITCFVGGSITAYAPVRWAHPALAIPATVPPTV
                     YIFFIESGPHPMAGFTALFFTAMVLYYAFRENELVARRLRADARNRRELRAIEDLAAS
                     CRAVPLLNENRGA"
BASE COUNT          729 a         1629 c         1729 g          767 t
ORIGIN      
        1 gccctgccac aggacctgct cggtatcgct catggtcgga tcgtcatcgg ggaaacgttc
       61 gggactgtac cgcaaatgcg ccgctcaacg ccgcaggtac tcgtcgcgga atttctgcag
      121 gcgcttggcg gaaaccggaa gcggcgtctt gagctcctgc gcgaaaagcg agacgcgcag
      181 ttcctccacc agccagcgga attcctcgag cctcgccccg cccttgcccg cctcgcggtc
      241 ggcctcgtac tgggcccaca gcccggcgac gacgggggcg tggcgcaggt cgcgctcggc
      301 gttggcacgg tacttctgca gcctgagcgc gccgcccttg aggtagcgcg gaatgtgctc
      361 gagcctctcc cacggcgtgc gcgcgaggaa tccgggatgg acgagctggt cgcgctgcgc
      421 cttcacctcg tgcgcaacct tgcccagcga ggcaccctgc gcgagcacag ccgtcagcgc
      481 gtggtgcgcc tcggcgatgg cgatcacgtg acgcgtgagc gcctcggtca cggccggcag
      541 gcgcgtccgt gcgcgcttct tctgctcctc gaacgacttc ggcgttcgcg gcgtttcgtc
      601 gtcgccgatg aaggcgcggt cgatgacggc cgcggctatg tcctcgcgca gcttctccgc
      661 gctcgtcacc gcctgcagct tgagggcaag ggcgttgaag ccctgcggcc cgcgctcgag
      721 gtacttcacc tgctcgcgca actcgaaggc caggagccgc ttcacgccgc cgcgatgcgc
      781 ctgcaccgcc ttgtccctcg tgtcgaagag ccggatggcc acgctcgcgc cttcgtcgac
      841 gagcgccggg taaccggtga gcgcctggtt gccgcgccgg aagctcacct ccgcgggcag
      901 gtcgccgaag tcccacgcag tgattccctc ccgttccagg ccgggcttcg attgggccag
      961 cgtgagctgc gcggcttcgc ccagctgccg gcgcagggcg gccaggtcgc ggcccatggc
     1021 gagttcgcgc ttcgcgtcgt ccaccacgcg caggttcatc gccaggtgcg cgggcgcatc
     1081 ttccctggac cagacatcgg cgtcgaggcg ctcgccggcc agccgggaag cgtaggcgag
     1141 gacttcctcg gcgatgggcc gctcgccggg gttcacgcgc tcgaggaatt tcgtgacgtg
     1201 ctccggcacc ggcaccagga gcgtgcgcac gcgcttgggc agcgccttca tcgtccacgc
     1261 caccttgtcc cgcaccatgc ccggcacgag ccagtcgatg accgcctcgt ccacctggtt
     1321 caggagcgcc agcggcacgt tcatcgtgac gccgtccatc gggtggccgg gctcgaagcg
     1381 ataggcgagg gcgaacgcat cgttgccgat gcgcagctgc ttcggaaaga gttcctcggt
     1441 caccgactcc gcgccgtggc gcatgagatc ggacttggcg aggaagagga gcttcgcgtc
     1501 ggccttcccc gcctcccggt accacgcctc gagcgaaacc gtgtcgcgca cgtccgcggg
     1561 caaccgcgcg gcatagaaac cgaagatcgc gcgttcgtcc acgagcacgt cctgccgccg
     1621 cgcgcggtgc tcgaggtcct ccacctcgcg gatgagcgcc cggttgtggg cgaagaacgg
     1681 gagcttcgtg tcgaactcgc cggcgaccag cgcgccttcg atgaagacct cgcgcgagcg
     1741 cgcggggtcg aggcggccgt agctcacctt gcggcgcgcg gcgatgacca gcccgtacag
     1801 cgccaccgac tcgtaggcca tcacctcgcc gcgggccctg tcccagtggg gctcggcgtg
     1861 ggtgcgcgtg acgaggtggg cggccgcctt ctcgatccac tcggggtcga tgcgcgcgac
     1921 atttcgcgcg aagacgcgcg tggtctcctg caattcgccc gccatcaccc agcgcggtcg
     1981 gcccttcttc aggcccgacc ccggccagat ggcgaacggg atgccgcgcg gcgcgttgta
     2041 gtggtcgccc tcctgcgcct tgaggccgac gttcgagatg aggccggtga ggagcgcccg
     2101 gtgcacctgc tcgtaggtgg cgtccgtcgt gttctcgcgg atcttcaggt cagcggccag
     2161 ctcgcgaagc tgcccggcca ggtcgcgcca ttcgcgcatg cgcacgtacg agaggaaatg
     2221 ctcgcggcag gtggcccaga gcttgcggtt ggattccttg tggtggaacg cctcgtcgaa
     2281 gaacttccac aggttgatga gctgctggaa gtcggaggac tcgtcgcgga actcctcgtg
     2341 ggcgcggtcg gattcctggc gcttgtcctg gggccggtcg cgcgggtcct ggatggagag
     2401 cgcggatgcc aggatcagca tctcgcgcag gcagttgaac tcgcgcgcgg caacgagcat
     2461 gcggccgatg cgcgggtcga gtggcagcct ggcgagctcg tggccgagcg gcgtgaggac
     2521 gcgcctttcg tcgatggcgc ccagctcgaa gaggctgcgg tagccgtcct cgatctgccg
     2581 cggcgtgggc ggctcgatga acgggaaggc cgcgatattg cccagctcga gtgccgccat
     2641 gcgcaggatc acgctcgcga gcgaggtgcg caggatctcc ggcgtggtga attcgggccg
     2701 ggcggcgaag ttctcctcgg cgtaaaggcg gatggccacg ccgctcgcca cgcgtccgca
     2761 acggccggca cgctgccggg ccgccgcctg cgaaaccggc tcgatgcgca gctggtcgat
     2821 cttctggcgg gggctgaagc gcttcacccg cgccagcccc gtgtcgatca cgtagtggat
     2881 tccgggaacg gtgagggagg tctcggcgac gttcgtggaa agcacgatgc gccggtgcgc
     2941 agccggctcg aagacgcggt cctgctcctc ggcggacagg cgcgagaaga gcggaagtat
     3001 ctcggcgccc ttgggatggt gcttgcgcag cgtctcggcc gcctcgcgga tctcgcgttc
     3061 gcccggcagg aagacgagga tgtcgccggt gcgggcctcg ctcgcgatct cgtcgaccgc
     3121 cctggcgatg gcctcgttca tgtccacggg ctcgtcgtcc tcgtcgtcga cctcgaagaa
     3181 ctccgggcgg tagcgcacct cgacgggcca ggtgcgtccc gagacctcga tcaccggcgc
     3241 gccgccgaag aaatccgaaa agcgctgcgt gtcgatggtg gcggaggtga cgatcacctt
     3301 gagttccggg cgcgcgacga cgaggcgctt gacgtacccc aggagaaaat cgatgttgag
     3361 gctgcgctcg tgcgcctcgt cgacgatgat ggtgtcgtac gcgcgcaatt cgcggtcaga
     3421 gtgcgtctcc gcgagcagga tgccgtcggt catcaccttc agcaccgtct ccggccccgt
     3481 gcggtcctgg aagcggatct tgtggccgac gaagccgcgg ggcgtgccgg gcaactcgtg
     3541 ggccaggcgc gtggcgaggc tgcgcgcggc gatgcggcgc ggctgcgtgc atccgatgag
     3601 gcccgcggcg ccgcgcttca gcgcgaggca gatcttcggg atctgcgtcg tcttcccgga
     3661 acccgtttcg ccgcagacga tggtgaccgg atgcttcgcc acgagggctg cgatgtcgtc
     3721 gcgcctctgt gcgatgggaa gatcgtcggg aaaacgcgga tcgggcagcc gcgcgaggcg
     3781 gcctgcatag gcattgcgcg atcgggcgag caggtcctcg agcagggacc gttcgcgctc
     3841 gcaggcccgc ccggcagcca tgcgggcgcg caccgtgtcg gcaagcgcgg cgagcttcgg
     3901 gtggtcggcc gcgagggtgg cggccaggag gtcttcggga agggcggtca agccgtcatt
     3961 ctattcggtg tcggactccg aacgcccctc acgccccccg gttctcgttc aggagcggca
     4021 ccgcccggca agaagcggcc aggtcctcga tggcccgcaa ctcgcgccgg ttgcgcgcat
     4081 ccgcccgcag gcgccgggcg accagctcgt tttcgcggaa ggcgtagtac aacaccatcg
     4141 cggtgaagaa aagggccgtg aacccggcca tcggatgcgg gcccgactcg atgaagaaga
     4201 tgtacaccgt gggcgggaca gtggcgggaa tcgcgagcgc gggatgggcc cagcgcacgg
     4261 gagcgtacgc cgtgatcgag ccgccgacga agcaggtgat caccatgatc gcgaaggtct
     4321 ggcggtagcc gggctcctcc gggaaaagcc aggtgcccag caatccccac tggatgccag
     4381 cgaggacgtt cccgagcacc gcaagctttg cccacagtcg cggctccagc gccgggccgc
     4441 gggcgtggtg aagccaggcg agccccaggc gcagtgcagc cacgagcgac ccgtacgcaa
     4501 gccacacgat gtgcgggccg cgaaggccgt cgtccagaaa taccgcgacg caaaggaggg
     4561 ttccgacata cgccgcggcg gccgataccc cgaggttgcc gaagagctcg gagacctgga
     4621 gcgcctcgat ttcctgtgac agggattgcg gggcccgctt ggggtgcccc ggctcggtag
     4681 acatgggtgc gcgcgcctct gggagtatcg acccccatcg ctgtcaatct gacgacagtt
     4741 aatcgcagta ggcgcccgga aactgtgaac caggtcacat ttccggcgtc gccgccggac
     4801 gcccgcgccc gctcagggat tgagctggcg gtagacgctc gtcgaaacgc ggga
//
LOCUS       DASUEV010000366         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_676426, whole genome shotgun sequence.
ACCESSION   DASUEV010000366 DASUEV010000000
VERSION     DASUEV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676426"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VFV55_11610"
     CDS             complement(<1..788)
                     /locus_tag="VFV55_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944993.1"
                     /translation="MRGPFRSVAAALFAAAALASHAQGEFLVPPASLVLDGLPPIPAE
                     IAAKAAPYTDFKPGTLLDWHPLRREILVRLRLGNADQLHRVAQPGATPDPLTDHPDAV
                     SAGRYEPRAGNWLLFTRGTGGDEAFRIYRLDPAMKEARPVSPAGRRASAPEWNHKADR
                     IVFTSVPVDRNNDSREARTTVHLADPGAPDEARELAELTGGGWFDFTFSPDDRLLAFV
                     EYVSAEESHVWLMDVATGKRRRLTPPTRGEAVFYANPQFSPDGKG"
     gene            complement(796..1647)
                     /locus_tag="VFV55_11615"
     CDS             complement(796..1647)
                     /locus_tag="VFV55_11615"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX4944994.1"
                     /translation="MKAELLVSTEMLAAHLDDPDWIVFDTRHDLVDTEKGRRAWKAAH
                     IPGAYFMHMDEDLSGVKTGRNGRHPLPPIGEFAARMNKCGVSPGRQVVVCDDGGGSFA
                     VRLWWMLRWLGHDRVALLDGGFAAWQREKRPVNAAVPAPREGQFEPRPMLGAIADMHY
                     VAAFRESPTVRIVDARAPVRFTGEQETIDPVAGHIPGSVNRFWQDNLAHDGRFLPPEE
                     LRAEFLELLGGAPPALAVHYCGSGVTACHNQFAMELAGLSGSRIYPGSWSEWCADPSK
                     PVATGPK"
     gene            complement(1637..2506)
                     /locus_tag="VFV55_11620"
     CDS             complement(1637..2506)
                     /locus_tag="VFV55_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX4944995.1"
                     /translation="MTSSWMLVAGFFFAAMALLVKLGGAHFESIELGFYRSLGAFLFI
                     AAFVVARRERVWNPHMGTHFLRSMAGTFGIITYFYGIANLPLATATTLNYTSPLFLAV
                     ATTVVLQEKFSRWLIVAIALGFAGVAMLLQPTFAAGKEGAAMVGLFSGLLASWAYLGV
                     RKLSQAGEPEWRILFWFGLFGTVGCAAWQVAVSTFHPIGWENAWILVGIGILGTAAQL
                     AMTRAYRTGNTLVVGSLSYSTIVFSTLFMVTIWEDRLTFLGWAGIVVIVASGLIALRV
                     ERKEHVEEAGFES"
     gene            2546..3364
                     /locus_tag="VFV55_11625"
     CDS             2546..3364
                     /locus_tag="VFV55_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4944996.1"
                     /translation="MVPARRLLLLLGLAVVLGGAWLAPLDSNASQHAQAGLKRALASF
                     ATARALNAVISVVQGTEVAVQPAGVGIVFTPGQVLDPVNDLVEQFSALMLAASVAFGL
                     QIALIKFGGYWAVSLALSAFALAWVAANWRRWSAPGWLTRMLVGMLLVRFAVPLVVLG
                     SEAGFRLFLENDYAAGQSSIELSTSRFASLSPPAPAPKADDSLPERFRQWWSQNADVG
                     KRIDELKEVAGRAVEHIVKLIVVFLLQTLVVPLLLFWVLFRIGRALAGIPARSP"
     gene            complement(3395..3481)
                     /locus_tag="VFV55_11630"
     CDS             complement(3395..3481)
                     /locus_tag="VFV55_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX4944997.1"
                     /translation="MKHYITNILQKLQVRSRVEAALLAARSK"
BASE COUNT          550 a         1147 c         1224 g          570 t
ORIGIN      
        1 cccttgccgt cgggcgagaa ttgcgggttg gcgtagaaca ccgcctcgcc cctcgtgggg
       61 ggcgtcaggc ggcggcgctt gcccgtggcc acgtccatca gccacacgtg cgactcctcg
      121 gccgacacgt actcgacgaa ggcgaggagc cggtcatcgg gcgagaaggt gaagtcgaac
      181 catccgccac cggtcaattc ggccagttcg cgtgcctcgt ccggcgcgcc cgggtcggcg
      241 aggtgcacgg tcgtccgcgc ttcgcgcgag tcgttgttgc gatcgacggg caccgaggtg
      301 aacacgatcc ggtccgcctt gtgattccat tccggcgcgc tcgcgcgccg gcccgccggg
      361 ctcacgggcc gtgcttcctt catggccgga tccaggcggt agatgcgaaa agcctcgtct
      421 ccgcctgttc cgcgcgtgaa aagaagccag ttgccggcgc ggggctcgta tcggccggcc
      481 gaaacggcat cgggatggtc ggtgagcgga tcgggagttg cgccgggctg cgcgacgcgg
      541 tggagctggt ccgcgttgcc cagcctcagc cgcaccagga tctcgcgccg caagggatgc
      601 cagtccagca gggtgcccgg cttgaagtcg gtgtagggcg cggccttcgc cgcgatctcc
      661 gcggggatcg gcggcaggcc gtcgaggacg aggctggcgg gaggaacgag gaattcgccc
      721 tgcgcgtgcg aggcaagggc ggcggccgcg aacagggcgg ccgcgacgct gcgaaaaggt
      781 cctctcaccg ccgcatcact tcggcccggt ggccaccggc ttcgacggat cggcgcacca
      841 ctcgctccag gagccgggat agatgcgcga gcccgacagg ccggcgagtt ccatcgcgaa
      901 ttgattgtgg caggctgtca cccccgaccc gcagtagtgc acggcgagcg ccggcggggc
      961 gccgcccagc agttcgagga attccgcgcg aagctcttcc ggcggcagga agcgtccgtc
     1021 atgggcgagg ttgtcctgcc agaagcggtt cacggaaccg ggaatgtggc cggctacggg
     1081 atcgatcgtt tcctgctcgc cggtgaagcg gaccggggcg cgcgcatcga cgattcgcac
     1141 cgtcgggctt tcgcggaacg ccgccacgta atgcatgtcg gcgatggcgc ccagcatcgg
     1201 ccggggttcg aactgccctt cgcgcggcgc gggcacggcg gcattcaccg gccgcttctc
     1261 gcgctgccag gcggcgaatc ctccgtcgag gagcgcgacg cggtcgtgcc ccagccagcg
     1321 cagcatccac cacaggcgca ccgcaaagct gccgccgccg tcgtcgcaga ccaccacctg
     1381 gcggccggga gacacgccgc atttgttcat gcgcgcggcg aactccccga tcgggggaag
     1441 gggatggcgg ccattcctgc ccgtcttcac gccggagagg tcctcgtcca tgtgcatgaa
     1501 gtacgcgccg ggaatgtgcg cggccttcca ggcgcggcgg cccttttccg tgtccacgag
     1561 gtcgtggcgc gtgtcgaaca cgatccagtc gggatcgtcc aggtgcgccg cgagcatttc
     1621 ggtcgagacg agcagttcag ctttcaaatc cggcctcctc cacgtgttcc ttcctttcca
     1681 cgcgaagggc gatgagcccg ctcgcgacga tgaccacgat gcccgcccag cccaggaacg
     1741 tgagcctgtc ttcccagatc gtcaccatga acagggtgga aaacacgatg gtcgagtagg
     1801 acagcgaccc caccaccagg gtattgcccg tgcggtaggc gcgcgtcatg gccagttgcg
     1861 cggccgtgcc caggatgccg atacccacca ggatccacgc attctcccag ccaatggggt
     1921 ggaaggtcga gacggcgacc tgccacgccg cgcaacccac ggtgccgaac aggccgaacc
     1981 agaagaggat gcgccattcc ggctcgcccg cctggctcag cttgcgcacg ccgaggtagg
     2041 cccacgaggc cagcaggccc gagaagaggc ccaccatggc agcgccctcc ttgccggcgg
     2101 cgaacgtggg ctgcaggagc atcgccacgc cggcaaagcc cagcgcgatg gcgacgatca
     2161 gccagcgcga gaacttctcc tgcagcacca ccgtggtcgc caccgcgaga aagaggggcg
     2221 aagtgtagtt gagcgtggtc gcggtggcga gcggcaggtt cgcgatgccg tagaagtagg
     2281 tgatgatccc gaaggtgccc gccatgctgc gcaggaagtg cgtgcccatg tgcgggttcc
     2341 agacgcgctc gcgacgggcc accacgaacg cggcgatgaa gaggaatgcg cccagcgagc
     2401 ggtagaagcc gagctcgatc gactcgaagt gcgcgccgcc gagtttcacg agcagggcca
     2461 tggcggcgaa gaagaagccc gccacgagca tccaggagga ggtcatccag ggggaatcca
     2521 gggaaccggc gggtatgata atcgaatggt tcccgcccgc cgattgctgc tgcttctggg
     2581 ccttgcggtc gtgctcggcg gcgcgtggct tgcccctctc gattcgaacg catcgcagca
     2641 cgcgcaggcc gggctgaagc gcgcgctcgc cagcttcgcg accgcgcgtg ccctgaatgc
     2701 ggtgatctcg gtggtgcagg gcaccgaagt ggccgtgcag ccggcggggg tgggcattgt
     2761 cttcacgccg ggccaggtgc tcgatcccgt caacgacctg gtcgagcagt tctcggcgct
     2821 gatgctcgcc gccagcgtcg cgttcggcct ccagatcgca ttgatcaagt tcggtggcta
     2881 ctgggcggta tcgttggccc tgtcggcatt tgctctcgcg tgggtcgcgg cgaactggcg
     2941 ccgatggtcg gcgccgggct ggttgacccg catgctcgtg ggaatgctgc tcgtgcgctt
     3001 tgccgtcccg ctcgtcgtcc tgggaagcga ggcgggtttc cggctgttcc tggagaacga
     3061 ctatgcggcg ggccagtcga gcatcgaatt gtcgacgagc cggttcgcca gcctttcgcc
     3121 gccggcgccg gcgcctaagg ccgatgattc cctgcccgaa agattcaggc agtggtggtc
     3181 gcagaacgcg gacgtcggca agcgaatcga tgaactgaag gaggtcgccg gtcgggccgt
     3241 cgagcacatc gtgaagctta tcgtcgtgtt tctcctgcag acgctcgtcg tgcccctgct
     3301 gcttttctgg gtgctgttca ggatcggccg ggctctcgcc gggattcccg ctcgcagtcc
     3361 gtgaattcct gcgcgcgcgc cgccggccct ggcgctactt gctgcgcgcg gccagcagcg
     3421 cagcctccac ccggctgcga acctggagtt tctgcaggat gttcgtgatg tagtgcttga
     3481 tggtcttctc g
//
LOCUS       DASUEV010000367         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_677562, whole genome shotgun sequence.
ACCESSION   DASUEV010000367 DASUEV010000000
VERSION     DASUEV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677562"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..631
                     /locus_tag="VFV55_11635"
     CDS             224..631
                     /locus_tag="VFV55_11635"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX4944998.1"
                     /translation="MTKRSEYVEYVIETMRAFGAVEAKAMFGGWGLYHQGAFFALVAG
                     DVLYLKTDHESRPRFEAAGLEPFVFQSRDGQKTAMSYRRAPDEALENPAVMAEWARYA
                     YEAALRAAAKKEKSRSAKAATPPRSPARPRRGN"
     gene            complement(588..1061)
                     /locus_tag="VFV55_11640"
     CDS             complement(588..1061)
                     /locus_tag="VFV55_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX4944999.1"
                     /translation="MSDNHTFTATVSWPADPAQKLPPDPAFSRNAVLACPGHAPVPGS
                     SPAVFGGDALRYNPEELMTLALAHCHMLTYLSLAAKKRVAVLAYEDRADGILGKDPES
                     GKMKLLSAHLHPRVTVARGANLEDARAMHAKAHEHCFIANSVNFPVEVAPEIVEA"
     gene            complement(1078..2259)
                     /locus_tag="VFV55_11645"
     CDS             complement(1078..2259)
                     /locus_tag="VFV55_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX4945000.1"
                     /translation="MQHARRNVFLLACCQALLFTNNVTLIALNGLAGFALADNKALAT
                     LPVTGYVVGAALSTFPASLWMKRVGRRNGFLTGTLFGIAGGAIATLGIWLSSFWTLVL
                     GTLVLGAYNAFGQYYRFAAADTVPVQWKAKAISLVMAGGLVGGIIGPEMSKHTRELVQ
                     PTFLASYASLIGFCLVTMAILSGLRIPDPQGEEVHGATRPLRQIMAQPVFIVAVVVGA
                     LGYGVMNLLMTSTPLAMGFCGLPYAAAASVISAHVIAMFGPSFFTGSLIQRFGVLNVM
                     MAGVVAMFACVAIALSGQLYLHFWLSLVLLGVGWNFMYVGGTTLLTEACRPAEKAKTQ
                     GANEILVFCTTATSSFTSGVLVNAAGWNTLNYVALPFLAIAGSAALWLAIRRRAAAAA
                     T"
     gene            2374..3345
                     /locus_tag="VFV55_11650"
     CDS             2374..3345
                     /locus_tag="VFV55_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX4945001.1"
                     /translation="MNYRRLGRSGLKVSELSFGSWVTYGNQLDTKSAAECMAAAWDMG
                     VNFFDNAEVYAAGESERIMGEVLKQLAWPRLKYIVSTKFFWGITDGPNAKNTLNRKYL
                     MQAIDASLARLQLDHVDLVYCHRPDPETPIEETVWAMHDMIRQGKALYWGTSEWSASE
                     IMAAWQIAERHHLAKPVVEQPQYNLFHRKRVEVEYRHLYSDIGLGLTTWSPLASGLLT
                     GKYRDGVPTDSRGAIPRMGFLVPELTDPKKNAVVGALDFIVKELGCTLPQFAIAWCAR
                     NPHVSSVITGASRVAQVKENLEAAQFVTKITPPMLERVEQIMAGHCE"
     gene            3445..4407
                     /locus_tag="VFV55_11655"
     CDS             3445..4407
                     /locus_tag="VFV55_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HEX4945002.1"
                     /translation="MQAVGTLGYLQISGWRAPVIDAVYMTFNAVSTIGFAETVDVSHG
                     IWGRLFTMAVGFFGIAGTWYLFSTFTAFILETNLNQAYRRRRMERTISALSGHYIVCG
                     IGRVGGYVADELLKTEREFVVIDTDAETLAAHEERTGHRIRLQGDAQEDDVLRRAGVA
                     RAAGVFAVTSDDSRNLVVSLSAKQLNPAVRVVARVHDRRNVDKTRRAGADEIVSPDFT
                     GGMRIASAMLRPSAVHVMDMMLHTEENLRVEEVLVPGGAAATTVDALGRRREWLLIAI
                     RDARSLWIFNPPGETRIAAGDTIVAMVSPEGRRLLEAAFSAPAG"
BASE COUNT          830 a         1514 c         1596 g          714 t
ORIGIN      
        1 tgtagatggg tggaaagtcg aggcctggct tgttctgcct ggcctcgact ttccgggcac
       61 ccatccgacg gtcaagcttg cgtgcccagg attgcgatcg atttccaccg ccaccgcctc
      121 gccctggtca accgggattg cgctcgactt ccaccgccac ctcttcaccc ggcattcctg
      181 tgccggcact cccggaattc ggtcaagcac ccataatggg cgcatgacaa agcgaagcga
      241 gtacgtggag tacgtcatcg agacgatgcg cgccttcggc gccgtcgagg cgaaggccat
      301 gttcggcggc tggggcctct atcaccaggg cgcgttcttc gcgctggtgg ccggcgacgt
      361 gctctacctc aagacggacc acgagagccg cccgcgcttc gaggcggcgg gactcgagcc
      421 cttcgtgttc cagtcgaggg acgggcagaa gaccgcgatg tcgtaccggc gcgccccgga
      481 cgaagcgctc gagaaccccg cggtgatggc cgagtgggcg cgttacgcct acgaagcggc
      541 gctgcgggcc gcggcgaaga aggagaagtc ccggtccgcg aaggccgcta cgcctccacg
      601 atctccggcg cgacctcgac ggggaaattg accgagttgg cgatgaagca atgctcgtgc
      661 gccttggcat gcatggccct cgcgtcctcg aggttcgcgc cgcgcgcaac cgtcaccctg
      721 gggtgcaggt gcgcggagag cagcttcatc ttccccgact ccgggtcctt gccgaggatg
      781 ccgtcggcgc ggtcctcgta ggcgagcacg gcaacgcgct tcttcgcggc gagcgagagg
      841 taggtgagca tgtggcagtg cgcgagggcg agcgtcatga gttcctcggg gttgtagcgc
      901 agtgcgtcgc cgccgaagac cgcgggcgat gaaccgggaa ccggcgcgtg gccggggcac
      961 gcgagcacgg catttcgcga gaacgccggg tcgggcggca gcttctgtgc gggatcggcg
     1021 ggccaactga cggttgccgt aaaggtatgg ttgtcgctca ttgcatggct ccttgtgtca
     1081 agtggctgcg gccgccgcgc ggcgacgaat ggccaaccat agcgccgcag accccgcgat
     1141 cgcgaggaac ggcagcgcca cgtaattgag ggtgttccag ccggccgcgt tcaccagcac
     1201 gcccgaggtg aacgacgaag tggccgtggt gcagaagacg aggatctcgt tcgctccctg
     1261 cgtcttggcc ttctccgccg gccggcaggc ttccgtgagg agcgtggtgc cccccacgta
     1321 catgaagttc cagccgacgc ccagcagcac gagcgacagc cagaagtgca ggtagagctg
     1381 tcccgagagc gcgatggcca cgcaggcgaa catcgcgacg accccggcca tcatgacgtt
     1441 gagcacgccg aagcgctgga tgagagaccc ggtgaagaac gacggcccga acatcgcgat
     1501 cacgtgcgcc gagatcacgc tcgccgccgc ggcgtagggc agcccgcaga agcccattgc
     1561 gagcggcgtg gaggtcatga ggaggttcat gacgccgtag cccagcgcgc cgacgaccac
     1621 ggcgacgatg aacaccggct gggccatgat ctgccgcagc ggccgcgtgg cgccgtgcac
     1681 ctcctcgcct tgcgggtccg ggatgcgcaa cccggaaagg atcgccatcg tcaccaggca
     1741 gaagccgatg agcgaggcgt aggaggcaag gaaggtcggc tgcacgagct cgcgcgtgtg
     1801 cttgctcatc tccgggccga tgatgccgcc cacgaggccg cccgccatga cgagcgaaat
     1861 ggccttggct ttccactgca ccggaacggt gtcggcggcc gcgaagcggt agtactgccc
     1921 gaaggcgttg taggcgccca gcaccagggt gccgaggacc agcgtccaga agctcgacag
     1981 ccagatcccc agcgtggcga tcgccccgcc cgcaatcccg aagagcgtcc cggtgaggaa
     2041 gccgtttcgc cttccgacgc gcttcatcca cagcgaggcg ggaaaggtcg agagcgcggc
     2101 acccaccacg tacccggtga cgggcagcgt ggcgagcgcc ttgttgtcgg cgagcgcgaa
     2161 ccccgccagc ccgttcagcg cgatcagcgt gacgttgttg gtgaagagaa gcgcctggca
     2221 gcaggcgagg aggaagacgt tgcggcgggc atgttgcatc agcgcattct aatgcgcggc
     2281 ggcaattcgc gattgacccg ggggcccggg acacggcagt ttccgcgaat ccgcgataat
     2341 ggtgccttcc ctcccgggct ttcccctccg acgatgaact acaggcgact cggccgttcc
     2401 ggcctcaagg tgagcgagct ctcgttcggc tcgtgggtga cctacggcaa ccagctcgac
     2461 acgaagtccg ccgccgaatg catggccgcg gcgtgggaca tgggcgtcaa cttcttcgac
     2521 aacgccgagg tttacgccgc cggggagagc gaacgcatca tgggcgaggt gctgaagcag
     2581 ctggcctggc cgcggctcaa gtacatcgtg tccaccaagt tcttctgggg catcaccgac
     2641 gggcccaacg cgaagaacac gctcaaccgc aagtacctga tgcaggccat tgacgcgtcg
     2701 cttgcacgcc tgcaactcga ccatgtcgac ctcgtgtact gccaccggcc ggaccccgag
     2761 acgcccatcg aggaaacggt ctgggcgatg cacgacatga tccggcaggg caaggccctc
     2821 tactggggaa cctccgagtg gagcgcctcc gagatcatgg ccgcgtggca gatcgcggag
     2881 cgccaccacc tcgccaagcc cgtggtcgag cagccgcagt acaacctgtt ccatcgcaag
     2941 cgcgtggagg tcgagtaccg ccacctctac agcgacatcg ggctgggact caccacgtgg
     3001 agcccgctcg cctcggggct gctcacgggc aagtaccgcg acggggtgcc cacggatagc
     3061 cggggtgcca tcccgcgcat gggattcctc gttccggaac tcacggaccc gaagaagaac
     3121 gcggtggtcg gcgcgctgga tttcatcgtg aaggagctgg gttgcacgct gccccagttc
     3181 gccatcgcct ggtgcgccag gaacccgcac gtctcctcgg tgatcaccgg ggcgtcgcgg
     3241 gtggcgcagg tgaaggagaa cctcgaggcg gcgcagttcg tgacgaagat cacgccgccg
     3301 atgctcgagc gtgtcgagca gatcatggcc gggcactgcg agtaggaagt cgccggagtc
     3361 catcccctgt tcaccctcat ccagatcttt cgcgaaagcc cctactaccc gaagctgcgg
     3421 agcgggatcc ttttcctgct tctcgtgcag gcggtcggca cgctgggcta cctgcagatc
     3481 tcggggtggc gcgcgccggt catcgacgcg gtctacatga ccttcaacgc cgtttcgacg
     3541 atcggcttcg cggagacggt cgacgtctcg cacggcatct gggggcgcct gttcaccatg
     3601 gcagtggggt tcttcggcat cgccggcacc tggtacctct tttccacctt caccgcgttc
     3661 atcctcgaaa ccaacctgaa ccaggcttac cggaggcgcc gcatggagcg caccatttcc
     3721 gccctcagcg ggcactacat cgtctgcggc atcggccgcg tgggcggcta cgtggccgac
     3781 gagctgctca agaccgagcg cgagttcgtg gtgatcgaca cggacgccga aacgctcgcc
     3841 gcgcacgagg agcgcaccgg gcaccgcatc cggttgcagg gcgacgcgca ggaggacgat
     3901 gtcctgcggc gcgccggggt ggcaagggcc gcgggagtgt tcgcggtgac gagcgacgat
     3961 tcgcgcaacc tcgtggtgag cctctcggcc aagcagctga atcccgccgt gcgcgtcgtc
     4021 gcccgcgtgc acgaccggcg caacgtggac aagacgcgcc gggccggggc ggacgagatc
     4081 gtctcgccgg acttcaccgg cggcatgcgc atcgcttccg ccatgctgcg gccgagcgcg
     4141 gtgcacgtga tggacatgat gctgcacacc gaggagaacc tgcgtgtgga agaagttctc
     4201 gtgccgggcg gagccgccgc gacgacggtc gacgccctgg ggcgccgccg cgagtggctg
     4261 ctgatcgcga tccgcgacgc acggtcgctg tggatcttca acccgccggg agagacgcga
     4321 atcgccgcgg gcgataccat cgtggccatg gtgagcccgg agggacggcg actgctcgag
     4381 gcggcgtttt cggcgccggc cgggtaaggc gggaaccaca gatgaataag gcggggaacc
     4441 acagatgaac acagttgaac acagataaga gcggattcat caatcgacaa caaccaagcc
     4501 aggagatgag gggatgaccc gctggatacg attcgaacac gaggctcgcg tgcagttcgg
     4561 cacgctcgag ggggagatca ttcgcgtcca cgagggcgac atgttcgcct cgccgcgcgc
     4621 caccggggcg agcgtgccgc gcgccgccgt gaag
//
LOCUS       DASUEV010000368         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_342583, whole genome shotgun sequence.
ACCESSION   DASUEV010000368 DASUEV010000000
VERSION     DASUEV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342583"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1771)
                     /gene="ureC"
                     /locus_tag="VFV55_11660"
     CDS             complement(68..1771)
                     /gene="ureC"
                     /locus_tag="VFV55_11660"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX4945003.1"
                     /translation="MSAKISRQAYAEMFGPTTGDRVRLADTDLWIQVETDLTVYGEEV
                     KFGGGKVIRDGMGQGQRLSAEVADTAITNALIVDHWGIVKADIGLKGGRIWKIGKAGN
                     PDIQPGVTIPIGAGTEAIAGEGMIVTAGGIDSHIHFICPQQIEEALMSGVTTMLGGGT
                     GPATGTYATTCTPGPWHIHSMLAAAEAFPVNLGFLGKGNASMPGPLREQVEAGAIGLK
                     LHEDWGTTPAAIDCALAVADEMDVQVAIHTDTLNESGFVEATIAAFKGRAIHTFHTEG
                     AGGGHAPDIIKVAGLPNVLPSSTNPTRPYTVNTIAEHLDMLMVCHHLDPAIAEDVAFA
                     ESRIRRETIAAEDILHDLGAFSMMSSDSQAMGRVGEVVIRTWQTAHKMRVQRGPAKGD
                     PKTHDNARVKRYIAKYTINPAITHGIAHAVGSIEEGKIADLVLWKPAFFGVKPSLILK
                     GGMIAAAAMGDPNASIPTPQPVHYRPMFGAFGGGLRTSVTFVSQAALANPVVQGLGLR
                     RPLEAVRNTRKIGKKDMVHNDWRPKIEVDAETYEVRADGELLACEPAKVLPMAQRYFL
                     F"
     gene            complement(1768..2076)
                     /locus_tag="VFV55_11665"
     CDS             complement(1768..2076)
                     /locus_tag="VFV55_11665"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX4945004.1"
                     /translation="MIPGEMKVAAGEIRINAGRGTLTLKVTNTGDRPIQVGSHYHFFE
                     TNEALAFDRARALGFRLNIAAGTAVRFEPGQSRTVELVALAGDRRVFGFQGKVMGALE
                     "
     gene            complement(2084..2386)
                     /gene="ureA"
                     /locus_tag="VFV55_11670"
     CDS             complement(2084..2386)
                     /gene="ureA"
                     /locus_tag="VFV55_11670"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX4945005.1"
                     /translation="MELTPREKDKLLVFTAALLAERRKAKGLKLNYPEAVAFITAGIL
                     EGAREGRGVSELMGWGATLLGRDEVMEGVPEMIPEIQVEATFPDGTKLVTVHHPIV"
BASE COUNT          348 a          860 c          822 g          446 t
ORIGIN      
        1 ccctttacct cgaccccgta cgcgggatgc ggcttcaccg gcaagcgggc gcggatctcg
       61 atcatcgtca gaacaggaag tagcgttgcg ccatgggcag cactttcgcc ggttcgcacg
      121 ccagcagttc cccgtcggcg cgcacttcgt acgtttcggc atccacctcg atcttcgggc
      181 gccagtcgtt gtgcaccatg tccttcttgc cgatcttcct cgtgttgcgc acggcttcca
      241 gcgggcggcg caggcccagg ccctgcacca ccgggttggc gagcgcggcc tgcgagacga
      301 aggtgaccga agtgcgcagc cccccgccga aggcgccaaa catcgggcga tagtgaaccg
      361 gctgcggtgt cggtatcgag gcgttcggat cgcccatcgc ggccgcggca atcatgccgc
      421 ccttgaggat cagggagggc ttgacgccga agaaggcggg cttccacagc acgaggtccg
      481 cgatcttgcc ttcctcgatc gagcccaccg cgtgcgcgat gccgtgcgtg atcgcgggat
      541 tgatcgtgta cttggcgatg tagcgcttca cccgcgcgtt gtcgtgcgtc ttcgggtcac
      601 ccttcgcggg cccgcgctgc accctcatct tgtgcgccgt ctgccaggtg cggatgacca
      661 cctcgcccac gcgccccatg gcctgcgagt cggaagacat catcgagaag gcgcccaggt
      721 cgtgcaggat gtcctcggcc gcgatcgttt ccctgcggat gcgcgactcg gcgaaggcca
      781 cgtcctcggc gatggccggg tccaggtggt ggcacaccat gagcatgtcg aggtgctcgg
      841 cgatggtgtt caccgtatag gggcgcgtcg ggttcgtgga cgagggcagc acgttgggca
      901 ggcccgccac cttgatgatg tccggcgcgt gcccgccgcc cgccccctcg gtgtggaagg
      961 tgtggatggc gcggcccttg aacgcggcga tggtcgcttc gacgaagccg gattcgttga
     1021 gcgtgtccgt gtggatcgcc acctgcacgt ccatctcgtc ggcaacggcg agggcgcagt
     1081 cgatggccgc cggcgtcgtg ccccagtcct cgtgcagctt gagcccgatg gcccccgcct
     1141 cgacctgctc gcgaagcggc cccggcatcg acgcgttgcc cttcccgagg aacccgaggt
     1201 tcaccgggaa ggcctcggcc gccgcgagca tcgagtggat gtgccagggt cccggcgtgc
     1261 aggtcgtcgc gtaggtgccg gtcgcgggac ccgtgccgcc gccgagcatc gtggtgacgc
     1321 cggacatcag cgcctcctcg atctgctgcg ggcagatgaa atggatgtgg ctgtcgatgc
     1381 cgccggcggt gacgatcatg ccctcgcccg cgatcgcttc cgttcccgcg ccgatcggaa
     1441 tcgtgacccc gggctggatg tcgggattgc ccgccttgcc gatcttccag atgcgcccgc
     1501 ccttgagtcc gatgtcggcc ttgacgatgc cccagtggtc gacgatgagc gcgttggtga
     1561 tcgccgtgtc cgcgacctcg gccgaaaggc gctggccctg gcccatgccg tcgcggatca
     1621 ccttgccgcc gccgaacttg acctcctcgc cgtacaccgt gaggtccgtt tcgacctgga
     1681 tccacaggtc cgtgtcggca agccgaacgc ggtcgcccgt ggtgggaccg aacatctccg
     1741 cgtaggcctg cctcgagatc ttcgcgctca ttccagcgcc cccatgactt tcccctggaa
     1801 gccgaacacc ctcctgtcgc ccgcgagcgc cacgagttcg acggtgcgcg actgccccgg
     1861 ctcgaaacgc accgcggtgc ctgcggcgat gttcaggcga aagcccaacg ccctggcgcg
     1921 atcgaaggcg agcgcctcgt tggtctcgaa gaagtggtag tgcgagccga cctggatggg
     1981 ccggtcgccg gtgttggtga ccttcaaggt gagcgtcccg cggcctgcgt tgatcctgat
     2041 ctcgccggcg gcgaccttca tttctccggg aatcatggct gcctcacacg atgggatggt
     2101 ggacggtgac gagcttggtg ccgtccggga acgtggcttc gacctggatc tcggggatca
     2161 tctcgggcac gccctccatg acctcgtcgc gcccgaggag cgtggcaccc caccccatca
     2221 gctcggaaac gccgcgcccc tcgcgcgcgc cctcgagaat gcccgcggtg atgaacgcca
     2281 cggcctccgg atagttgagc ttgagtccct tcgccttgcg tcgctcggcg agcagcgcgg
     2341 cggtgaagac gaggagcttg tccttctccc gtggcgtgag ttccatcgcg tccccttcag
     2401 gttctccaga tgcgcggcgt cgccgccccg cgcccggcca ccgccggacg cacccgttcc
     2461 cacagcgcgg cgaaat
//
LOCUS       DASUEV010000369         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_212812, whole genome shotgun sequence.
ACCESSION   DASUEV010000369 DASUEV010000000
VERSION     DASUEV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212812"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="VFV55_11675"
     CDS             complement(<1..1666)
                     /locus_tag="VFV55_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX4945006.1"
                     /translation="MLDALITWSARNRFLVLLATIAVTAAGIYAVVKTPLDALPDLSD
                     VQVIIYTEFPGQAPQVVEDQVTYPLTTSMLSVPKSKVVRGFSFFGASFVYVIFEDGTD
                     IYWARSRVLEYLNFAAGKLPRGVTPQLGPDATGVGWVYQYAVLGKNRTLAELRTLQDW
                     FIRYQLTKAHGVAEVASVGGFVQQYHVIVDPVKLRSYGVPLMKVSQVIRDSNRDVGGR
                     VIEMAEAEYMVRGRGYLRGKADIEGLVVKSEKGTPVLVRDIARVEMGPDERRGIAELN
                     GDGEMVSGIVVARFGQNALDVIANVKAKMAEIASGLPEGVTLEAVYDRSDLIHRAIDT
                     LKSTLLEESAIVAAVCIVFLLHVRSALVAIVMLPIGVLIAFLAMRALGINSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKHIERLKPGESRAEAMVTACREVGPALFFSLLIITVSF
                     LPVFTLESQEGRLFSPLAYTKTFAMAGAAILSVTLVPVLMGLFIRGKILPESRNPVNR
                     VLIALYRPIIAAVMRWKVTTLVLAVVVLGLSVIPAARLGSEFMPRLN"
     gene            complement(1668..3227)
                     /locus_tag="VFV55_11680"
     CDS             complement(1668..3227)
                     /locus_tag="VFV55_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX4945007.1"
                     /translation="MKTGAAIVVGVVVVAAAAGGFFVGTRQPAKPAPQVSATGVPAGE
                     AKKERKLLYYRNPMGLPDTSPVPKKDPMGMDYTPVYEGEEPPASGNELRISTERVQKL
                     GVRTQLAERRALDHVVRASGRVEVNERLLATISPKFEGYVERLHVNTTGQPVVKGQPL
                     FEAYSPELVSAQREYAIAARGMASLREASAESRSGMAQLAESSLTRLRNWDVSDEQIA
                     ELARTGEAKRSVTFRSPATGIVMEKKAVSGMRFMPGEMLYQVADLSHVWVIADVNEQD
                     IGQVTNGRKAQVQINAYPDKRFEGVVTYIYPTLKTETRTVSVRLELANPGGLLKPGMY
                     AQVELASGNSAKVIAVPVSAVIDSGLRRVVIVQTGEGRFEPRQVELGARSEEWIEIRS
                     GLKEGEPVVVTANFLIDAESNLRAALGGLGGPGPGVSHGAEGTLDAIDAKTGMVTISH
                     GPVPSLKWPGMTMDFALANAALVEGVKPGSAIAFEFVERKPGEWVITAVKAGAAKAKG
                     TVVPADPHKGH"
     gene            complement(3224..4543)
                     /locus_tag="VFV55_11685"
     CDS             complement(3224..4543)
                     /locus_tag="VFV55_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX4945008.1"
                     /translation="MSPEFPVRIARTHPIVPPLRRMAAACLAVLALASGPSLAQSALP
                     GRNVESFLEIAREKNPEFAAMRLEAAAARERPESAGALPDPTVRVELMDITNAGTEAG
                     PSLLPSRIGGTKYTVMQMLPWFGKRDAMREAAEADAQAASSRVDVTWLELAMRIKSAY
                     AQYYVAAENEKLTRGVLDLMAQLESVAQSRYAGGLAAQQDAIRAQVEQTSMRTDLVVL
                     ESERRRAAARLNALVARPGSAPIADPERIRPLPPPARLQIADLEERARARNPVIAVEE
                     ARARSAGKMVELADLNRYPDLTVGLSPTQTRNRISEWEIMFEVSIPLQQGSRRAKERE
                     ARAMLDAARAKRDAASTSSLGELSESLAGLEAARKTEALAATSLLPQAELTFQSALAA
                     YENGKVDFATLLDAQRQILRAKQERLKAQAEAQMRLAEIERIVGEDL"
     gene            complement(4607..4948)
                     /locus_tag="VFV55_11690"
     CDS             complement(4607..4948)
                     /locus_tag="VFV55_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4945009.1"
                     /translation="MIAFVFAQCLVSAHACDARQFGRSGEVVAHIEGCPGAAQDEVAG
                     DNLCEQHCQYGHASLDSNPPAPMAMNVSGPVFRVEAVAADCMAVSPPSRRLVPAFASP
                     PPAILFGVLRI"
BASE COUNT          819 a         1733 c         1712 g          859 t
ORIGIN      
        1 cgttgagccg gggcatgaac tcgctgccca ggcgcgcggc cgggatcacg ctcaggccca
       61 gcaccaccac ggcgagcacg agcgtcgtca ccttccagcg catcaccgca gcgatgatcg
      121 ggcgatagag cgcaatcagc acgcggttga ccgggttccg cgactcggga aggatcttgc
      181 cgcggatgaa gagccccatc agcaccggaa ccagggtcac cgacaggatc gcggcacccg
      241 ccatggcgaa ggtcttggtg taggcgagcg gcgagaagag gcggccttcc tgggattcca
      301 gcgtgaagac cggcaggaag gacacggtga tgatgaggag tgaaaagaac agcgccggcc
      361 ccacctcccg gcacgccgtc accatcgctt ccgcgcggga ttcgcccggc ttcaggcgct
      421 cgatgtgctt gtgcgcgttc tcgatcatga cgatggccgc gtccaccatg gcaccgatgg
      481 cgatggcgat gccgccgagg ctcatgatgt tggagttgat gccgagcgcg cgcatggcga
      541 ggaaggcgat gagcacgccg atgggcagca tcacgatcgc caccagggcg ctcctcacgt
      601 ggagcaggaa cacgatgcac accgccgcga cgatggcgct ttcctcgagg agggtggact
      661 tcagcgtgtc gatcgcgcgg tggatgaggt cggagcggtc gtagaccgcc tcgagcgtga
      721 cgccctcggg caggccggaa gcgatctccg ccatcttggc cttcacgttg gcgatcacgt
      781 cgagcgcgtt ctggccgaag cgcgccacga cgatgcccga caccatctcg ccatcgccgt
      841 tgagttccgc gatcccgcgc ctctcgtccg gacccatttc cacgcgcgcg atgtcgcgga
      901 cgagcaccgg cgtgcccttc tcgctcttca cgacgaggcc ctcgatgtcc gccttgccgc
      961 gcaggtagcc gcggccgcgc accatgtact ccgcttccgc catctcgatc acgcgcccgc
     1021 ccacgtcgcg gttggagtcg cggatcacct gcgagacctt catgagcgga acgccatagc
     1081 tgcgcagctt caccggatcg acgatcacgt ggtactgctg cacgaagccg ccgacgctcg
     1141 ccacctccgc cacgccgtgc gccttggtga gctggtagcg gatgaaccag tcctggaggg
     1201 tgcgcaattc ggcgagcgtg cggttcttgc ccagcacggc gtactggtac acccagccca
     1261 cgccggtcgc atccggcccg agctgtggcg tcacgccgcg cggcagcttt ccggcggcga
     1321 aattgaggta ttccagcacg cgcgatcgtg cccagtagat atcggtgccg tcctcgaaga
     1381 tcacgtacac gaaggaggcg ccgaagaagg agaagccgcg caccaccttc gacttgggca
     1441 ccgagagcat cgaggtggtg agcgggtagg tgacctggtc ctccacaacc tgcggcgcct
     1501 ggccgggaaa ctcggtgtag atgatcacct gcacgtcgga aaggtccggc agcgcgtcca
     1561 gcggcgtctt cacgaccgcg tagatgcccg cagccgtgac ggcgatcgtc gccagcagga
     1621 cgaggaagcg attcctcgcc gaccaggtga tgagggcatc gagcatctca atgccccttg
     1681 tgcggatcgg ccggaacgac cgtgcctttc gctttcgccg cccccgcctt gacggcggtg
     1741 atgacccatt ccccgggctt cctctcgacg aactcgaagg cgattgcgga gccgggcttg
     1801 acgccctcca cgagcgcagc gttggccagt gcgaaatcca tcgtcatccc gggccacttg
     1861 agggagggca ccggaccgtg cgaaatggtc accatccccg tcttcgcgtc gatcgcgtcc
     1921 agcgtgcctt ccgcgccgtg gctcaccccg ggcccggggc cgccgaggcc cccgagcgcg
     1981 gcgcgcaggt tgctttcggc gtcgatgagg aagttggcgg tgaccaccac cggctcgcct
     2041 tccttcaggc cggaacggat ctcgatccac tcctcgctgc gtgcgcccag ctcgacctgg
     2101 cgcggctcga agcggccttc gcccgtctgc acgatgacca cgcgacgcag gcccgagtcg
     2161 atcaccgccg aaacgggcac cgcgatgacc ttcgccgagt tgccggacgc gagctcgacc
     2221 tgcgcgtaca tccccggctt gaggagcccg ccggggttgg cgagctcgag gcgcacggac
     2281 accgtgcgcg tctccgtctt gagcgtgggg tagatgtagg tgaccacgcc ctcgaagcgc
     2341 ttgtcggggt aggcattgat ctgcacctgc gccttgcggc cgttggtgac ctggccgatg
     2401 tcctgttcgt tgacgtctgc aatcacccat acgtgggaga ggtccgcgac ctggtagagc
     2461 atctcgccgg gcatgaagcg cattcccgaa accgccttct tctccatcac gatgcccgtc
     2521 gcgggagagc ggaacgtgac gctgcgcttc gcttcgcccg tgcgcgcaag ctccgcgatc
     2581 tgctcgtccg agacgtccca gttgcgcagc cgcgtgaggc tcgattcggc gagctgcgcc
     2641 atgcccgagc ggctttccgc actcgcttcc ctgagggagg ccatcccccg cgccgcgatg
     2701 gcgtactcgc gttgcgccga gaccagctcc gggctgtagg cctcgaacag cggctggccc
     2761 ttcaccaccg gctgcccggt cgtgttcacg tgcagtcgct cgacgtagcc ctcgaatttc
     2821 ggcgagatgg tggcgaggag ccgctcgttc acctcgacgc ggcccgaagc gcgcacgacg
     2881 tggtccagcg cccggcgttc ggcaagctgc gtgcgcacgc ccagcttctg cacacgctcc
     2941 gtggaaatcc tgagctcgtt gcccgaggca ggcggttcct ccccctcgta gaccggggtg
     3001 tagtccatcc ccatcggatc cttcttgggc accggcgacg tgtcgggaag ccccatcgga
     3061 ttgcggtagt agaggagctt cctttccttc ttcgcttcgc ccgccggcac acccgtggcg
     3121 gaaacctgcg gcgccggttt cgcgggctgg cgagtaccga cgaagaatcc gcccgcggcg
     3181 gcggccacga ccacgacacc cacgacgatt gccgctcccg tcttcataga tcctctccca
     3241 cgatgcgttc gatctcggca aggcgcatct gcgcctcggc ctgcgccttg agccgctcct
     3301 gcttcgcgcg aaggatctgg cgctgcgcgt caaggagggt ggcgaagtcg accttgccgt
     3361 tctcgtaggc ggcgagcgcc gactggaacg tgagctcggc ctgcggcagc aggctcgttg
     3421 ccgcgagcgc ctcggtcttg cgcgccgcct ccaggccggc gaggctctcc gaaagctcac
     3481 ccagcgagct cgtcgaggcc gcatcgcgtt ttgcgcgcgc ggcgtcgagc atcgcgcgcg
     3541 cttcgcgttc cttggcacgg cggcttccct gttgcagggg aatgctcacc tcgaacatga
     3601 tttcccattc cgaaatccgg ttcctcgtct gggtcggcga gaggccgacg gtgaggtccg
     3661 ggtaacggtt gaggtcggcg agctcgacca tcttgcctgc ggagcgggcg cgtgcttcct
     3721 cgacggcgat caccggattc cttgcacggg cacgttcctc gaggtcggcg atctgcagcc
     3781 gcgccggcgg cggcaatggc cggatgcgtt cggggtcggc aatcggcgcc gaaccgggtc
     3841 gcgcgacgag cgcattgagg cgtgccgccg cgcggcgtct ttccgattcg agcaccacca
     3901 ggtccgtgcg catgctggtc tgctccacct gggcgcggat cgcatcctgc tgcgcggcaa
     3961 ggccgcccgc gtagcgcgac tgcgccacgc tctcgagctg tgccatgagg tcgagcaccc
     4021 cgcgcgtgag cttctcgttc tcggcggcga cgtagtactg cgcataggcc gacttgatgc
     4081 gcatggcgag ctcgagccac gtaacgtcca cgcggctcga ggccgcctgt gcgtccgcct
     4141 ccgccgcttc gcgcatcgcg tcgcgcttgc cgaaccaggg cagcatctgc atcaccgtgt
     4201 acttggttcc gccgatgcgc gatggaagga ggctggggcc cgcttccgtg ccggcattgg
     4261 tgatgtccat gagctcgacc cggaccgtcg gatcgggcag ggctcccgcg gattcggggc
     4321 gctcgcgtgc cgcggctgcc tccaggcgca tggcggcgaa ttcggggttc ttctcgcgcg
     4381 cgatctccag gaagctttct acgttgcggc cgggcagtgc cgactgcgcg agtgacgggc
     4441 cgctcgccag agcgagcacg gctagacatg cagcggccat ccggcgaagc ggcgggacga
     4501 tgggatgcgt ccgcgcgatg cggacaggga attcaggcga catggaacct ccggacgcga
     4561 ttgccgacgg tcgcgaaggc cgcgaccgtg atgaatcact gcgaggtcag atcctgagga
     4621 cgccgaagag gatggcggga gggggcgatg cgaacgcggg gacgaggcgc cgcgaaggcg
     4681 gcgaaaccgc catgcaatcg gctgccaccg cttcgaccct gaacacgggt cccgacacgt
     4741 tcatcgccat cggggcgggc gggttgctgt cgagcgacgc gtgaccgtac tggcaatgct
     4801 gctcgcagag gttgtcgcct gctacctcgt cctgcgccgc accgggacat ccctcgatgt
     4861 gagccactac ttccccactc cggccgaatt gcctcgcgtc gcaggcatgc gccgagacca
     4921 ggcattgcgc gaaaacgaac gcgatcatgg cgaacagggc ggcggctggg cgggagcggc
     4981 gagtcatggc cgtgaagtat gccccgggac ggaccggggg tcctttgatc gtacgcaacc
     5041 gcccggaatt ctgaccaatt cgtaatccag gtgacagatt ccggaaaagg ggtcaggtta
     5101 ctttgcacaa aaaataggca aaa
//
LOCUS       DASUEV010000370         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_683909, whole genome shotgun sequence.
ACCESSION   DASUEV010000370 DASUEV010000000
VERSION     DASUEV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683909"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1386)
                     /locus_tag="VFV55_11695"
     CDS             complement(1..1386)
                     /locus_tag="VFV55_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX4945010.1"
                     /translation="MADAPPRGRAWFLIVLTAVIAAGAAAGGVALLVNIWERKVEARN
                     PFYRVVELTDDTVDPVVWGKNFPSQFDGYKRTVDQVRTRYGGSEAVPRTPTQADPRSI
                     VAQSRLEEDPRLKTMWAGYAFSRDFREERGHAYMLDDQTFTERQQVVRQPGTCLHCHA
                     SVYVPYKKLGDGDLIKGFEKMNQMPFVEARRLVTHPVSCIDCHAPDTMQLRVTRPGFI
                     EGIRALKVSQGVKDYDVNTMATRQEMRAYVCGQCHVEYYFKGPEKRLVYPWANGLKVE
                     NILAYYDKEGFKDWTHKDSGAPALKAQHPEFELWSQGIHARSGVTCADCHMPYRREGA
                     LKISDHHVRSPLLNVNRACQTCHKWPEEELKTRAQTIQQRTFELRNRAMDALVALIGD
                     IKGAAVAGRTDAQLGPARDFQRKAQFYLDFVEAENSTGFHAGQEAARILGESIDFSRQ
                     GQVAVRDLAKR"
     gene            complement(1423..1851)
                     /gene="nrfH"
                     /locus_tag="VFV55_11700"
     CDS             complement(1423..1851)
                     /gene="nrfH"
                     /locus_tag="VFV55_11700"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HEX4945011.1"
                     /translation="MILALTVGLAAGIGAFTFVYARGASYLTNDPAACANCHIMREQY
                     DGWIASSHRAVAACNDCHTPHALVPKYATKALNGFWHSFAFTTGRFHEPIQINARNRA
                     ITEAACRSCHADIVHAIDAAPKPGRQLSCIACHNSVGHLH"
     gene            complement(2077..2151)
                     /locus_tag="VFV55_11705"
     tRNA            complement(2077..2151)
                     /locus_tag="VFV55_11705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2117..2119),aa:Gly,seq:ccc)
     gene            complement(2196..>2735)
                     /locus_tag="VFV55_11710"
     CDS             complement(2196..>2735)
                     /locus_tag="VFV55_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4945012.1"
                     /translation="KADVARAKKLLAEAGHPNGIELTLHGPQGRYVRDKEVAEAVTGQ
                     LTKAGIKTTLKTYEFVNYLNNMVYVHKAGPVWLIGWGTNSMDAETVYGPLFKAPTIFA
                     NWHNEDFNRMFEEAQSIMDEKKRREQYHRINKLWVEEMPAVPLYQQIDLYGVNKRVGW
                     KARPDERLKAFDMTVKDGK"
BASE COUNT          382 a          951 c          908 g          494 t
ORIGIN      
        1 ctaccgcttc gcgaggtccc gcaccgccac ctggccctgg cgcgagaagt cgatcgactc
       61 gccgagaatg cgcgccgcct cctgccccgc gtggaagccc gtggagttct cggcctcgac
      121 gaagtcgagg tagaactgcg ccttgcgctg gaagtcgcgc gcgggcccga gctgggcatc
      181 ggtgcggccc gccacggcgg cgcccttgat gtcgccgatg agcgcgacca gcgcgtccat
      241 cgcgcggttc cgcagctcga acgtccgctg ctggatcgtc tgcgcgcgcg tcttcaactc
      301 ctcctcgggc cacttgtggc acgtctggca ggcgcgattc acgttcagca gcgggctccg
      361 cacgtggtgg tcgctgatct tgagcgcccc ctcgcggcgg tacggcatgt gacagtcggc
      421 gcacgtgacg ccggaccgcg cgtggatgcc ctggctccac agctcgaact ccggatgctg
      481 cgccttcagc gccggcgcgc cggagtcctt gtgcgtccag tccttgaacc cctccttgtc
      541 gtagtacgcc aggatgttct cgaccttgag gccgttcgcc cacgggtaga cgaggcgctt
      601 ctcgggcccc ttgaagtagt actcgacgtg gcactggccg cagacgtagg cgcgcatctc
      661 ctgccgcgtc gccatcgtgt tgacgtcgta gtccttcacg ccctgagaga ccttcagggc
      721 gcggatcccc tcgatgaagc ccggccgcgt gacgcgcagc tgcatggtgt cgggcgcgtg
      781 gcagtcgatg cagctcaccg gatgcgtcac cagccggcgc gcctccacga acggcatctg
      841 gttcatcttc tcgaagccct tgatgaggtc gccgtcgccg agcttcttgt aggggacgta
      901 aacggacgcg tggcagtgca ggcacgtgcc gggctgccgc acgacctgct gacgctcggt
      961 gaaggtttga tcgtcgagca tgtaggcgtg cccgcgttcc tcccggaagt cccgcgagaa
     1021 cgcatagccc gcccacatcg tcttgaggcg cgggtcctcc tcgagccgcg actgcgccac
     1081 gatggaccgc ggatcggcct gggtcggcgt gcgcggcacg gcctcgcttc cgccgtaccg
     1141 cgtgcgcacc tggtccacgg tccgcttgta gccgtcgaac tgcgacggga agttcttgcc
     1201 ccagaccacc gggtccaccg tgtcgtcggt gagctcgacc actcggtaga acgggttgcg
     1261 ggcttcgacc ttccgctccc agatgttcac gagcagcgcg acgccaccgg ccgccgcgcc
     1321 ggcggcgatc acggcggtca gcacgatcag aaaccacgcg cgtccgcgcg ggggcgcgtc
     1381 agccatgaac cggtcctccc tgacatcggc gccacccgcg cttcaatgca ggtgcccgac
     1441 ggaattgtga caggcgatgc acgagagctg ccgccccggc ttcggtgccg cgtcgatcgc
     1501 gtggacgata tcggcgtggc acgaccggca ggctgcttcg gtgatcgccc ggttcctcgc
     1561 gttgatctgg atgggctcgt ggaaccgccc cgtcgtgaag gcgaacgagt gccagaagcc
     1621 gttgagcgcc ttcgtcgcgt acttcgggac cagcgcgtgc ggcgtgtggc agtcgttgca
     1681 ggcggccacc gcgcgatggc tcgacgcgat ccagccgtcg tactgctcgc gcatgatgtg
     1741 gcaattcgcg caggccgcgg gatcgttggt gaggtacgac gcgccccgcg cgtagacgaa
     1801 ggtgaacgcg ccgatgccgg ccgccaggcc gaccgtcagc gccagaatca cgagcccgac
     1861 cggtggacgc cccatcctcc cgcgcgcagc ttacacgacc cgcgcacaca gcagcacccc
     1921 cgggttcacc cggggcgcgc cgaaggctgg ggggtgtggg ggggccatat cgggcccctc
     1981 gcgtcttacg cgtttaaaga ggtgcgcccc ggttcacccg gggcgcgccg caggctgggg
     2041 ggtgtggggg gccatatcgg ggccccccac ctatattgga gcggccgacc ggattcgaac
     2101 cggcgacgtc cagcttggga agctggcatt ctgccactga attacggccg ctcagcgcct
     2161 cctatattac cctctcgtgg cggtcgctcg ccgcgctact tgccgtcctt gaccgtcatg
     2221 tcgaaggcct tcagccgctc gtcggggcgc gccttccagc cgacccgctt gttcacgccg
     2281 tagagatcga tctgctggta gagcggcacc gccggcatct cctcgaccca cagcttgttg
     2341 atgcggtggt actgctcgcg gcgcttcttc tcgtccatga tggactgcgc ctcttcgaac
     2401 atccggttga agtcctcgtt gtgccagttc gcgaagatcg tcggcgcctt gaagagcggc
     2461 ccgtagacgg tctccgcgtc catggagttc gtgccccagc cgatcagcca gacgggcccc
     2521 gccttgtgca cgtacaccat gttgttcagg tagttcacga actcgtacgt cttcagcgtg
     2581 gtcttgatgc ccgccttggt gagctggccc gtgaccgcct cggcgacttc cttgtcgcgc
     2641 acgtagcggc cctgcggccc gtgcagcgtg agctcgatgc cgttcgggtg gccggcctcc
     2701 gcgaggagct tcttcgcgcg ggccacgtcg gcctt
//
LOCUS       DASUEV010000371         5083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_684954, whole genome shotgun sequence.
ACCESSION   DASUEV010000371 DASUEV010000000
VERSION     DASUEV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684954"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VFV55_11715"
     CDS             <1..1206
                     /locus_tag="VFV55_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX4945013.1"
                     /translation="GFRINCPESAETVITDMREIGPSYYFAPPRVLEALLTQVTIRME
                     DAGRIKRWMFRHFMELARRVGSRILDGRPVGLGERVQYALGGLFVYGPLRNALGMSRI
                     RVAYTAGEAIGPDLFVFYRSLGINLKQLYGQTETCASVCVQPNGQVKSDTVGIPLEGV
                     EVRVLDSGEIVLRCPGLFVEYYRNEAATREAKDAEGWYHTGDAGYFDTSGHLKIIDRA
                     KDVGKLLDGTLFAPKYLENKLKFFAAIKEAVAFGDGRPEATAMINIDMQAVGDWAERR
                     GIAYSGYQDLSAKPQVYELIRECVEKVNADLSADPKLGGSQIHRFVVLHKELDADDGE
                     LTRTRKVRRRFIAEKYEPIVHALYGDMDHCWVEAQVRFEDGRTGTVRAQLAVREAKTF
                     PPQAARLAA"
     gene            1217..2008
                     /locus_tag="VFV55_11720"
     CDS             1217..2008
                     /locus_tag="VFV55_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX4945014.1"
                     /translation="MSDTERQFGEVILKLEGISLAFGGVKALTDITFDVREHEIRAII
                     GPNGAGKSSMLNVINGVYQPQQGKVTFRGETREAMEPHHAAQLGIARTFQSLALFKGM
                     SVLDNLLAGRNLMIRSNIFLQALRVGPALREEVQHRKRVEEIIDFLEIQHVRKTPVGR
                     LPYGLQKRVDLGRALAAEPKVLLLDEPMAGMNVEEKQDMCRFILDVNDEFGTTIVLIE
                     HDMGVVMDISDRVVVLDYGKLIGDGTPDEVRANQAVIDAYLGVKH"
     gene            2098..2994
                     /locus_tag="VFV55_11725"
     CDS             2098..2994
                     /locus_tag="VFV55_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007246480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4945015.1"
                     /translation="MPQWLEVVLGGLMSGVLYSLVALGLVLIFKASGVFNFAQGAMVL
                     FAALSLVRVMEKVPFAVALVITVVIMVAVAWLIERLVLRPLVNQEGIVLFMATLGIAY
                     FLDGLGQTIWGSDVYTLDVGMPKEPKVLLENVFEGGILVGMEDVIAAFISAALVALLA
                     LFFQKTGTGRALRAVADDHQAAQSIGIPLNRIWVIVWSVSGIVALVAGIIWGSKLGVQ
                     FSLQLVALKALPVVILGGFTSIPGAIIGGLIIGLGEKLSEVYLGPYLGGGIENWFAYV
                     LALVFLLFRPEGLFGERHIDRV"
     gene            2997..4061
                     /locus_tag="VFV55_11730"
     CDS             2997..4061
                     /locus_tag="VFV55_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX4945016.1"
                     /translation="MIYRETGQYKSSYEADQQLFPILQDKVFILLVLAFVFGAVPFLA
                     SEYLFRAILIPFLILTLAAIGLNILVGFCGQVSLGTGGFMAVGAYAAYNLAIRLPDLN
                     LLVVFLLAGVAATVVGVLFGLPSLRIKGFYLAVATLAAQFFLDWAFARIGWFTNNAPS
                     GSVTAPAITIFGYTFTSPVQKYLLVLSFVCVFALVARNLTRGAIGRSWMAMRDMDVAA
                     EVIGIRPLYAKVTAFAVSSFFVGLAGALWAFVYLGAWEPLAFDVNRSFQLLFMVIIGG
                     LGSILGSFLGAAFIVILPLVLNQAPGWLGMDLSTALISHIEFMIFGALIIFFLIVEPH
                     GLARLWAIGKEKLRLWPFPH"
     gene            4129..>5083
                     /locus_tag="VFV55_11735"
     CDS             4129..>5083
                     /locus_tag="VFV55_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX4945017.1"
                     /translation="MKASIKRKAAAAAIAVAGVLGFAQEAVAADEIFVPVLVYRTGAY
                     APNGVPWADGFVDYLKLVNERDGGVNGVKIAYEECETGYATDRGVECYERLKSKKPVA
                     VNPLSTGITFALTEKVAADKITLMTTGYGRSESADGSVFAWNFPLLGTYWSAADMLVQ
                     HVAKKGSLKGKKIALVYHDSPYGKEPIKILEEHAKKHGFTLLKLPVTHPGVEQKATWL
                     QIRQQRPDYVFLWGWGVMNSTSIKEAIAVSYPRLQIFGVWWSSAEPDVLPAEAAAKGY
                     NGITLGHSNERTLPVHKDIAKHLYDKGKGTAAKKELVGDVLY"
BASE COUNT          825 a         1670 c         1673 g          915 t
ORIGIN      
        1 ggcttccgga tcaactgccc cgagtcggcg gagaccgtga tcacggacat gcgcgagatc
       61 gggccttcgt attacttcgc gccgccacgc gtcctggagg cgctgctcac gcaggtgacg
      121 atccgcatgg aggacgcggg ccggatcaag cgctggatgt tccggcactt catggagctc
      181 gcgcggcgcg tgggctcccg gattctcgat gggcgacccg tggggctggg cgagcgggtg
      241 cagtacgcgc tcggcggcct gttcgtctac ggtccgctgc gcaacgcgct gggcatgtcg
      301 cgcatccgcg tcgcctacac ggccggcgaa gccatcggcc ccgacctctt cgtcttctat
      361 cgctcgctcg gcatcaacct gaagcagctc tacgggcaga ccgagacctg cgcatcggtt
      421 tgcgtccagc ccaacgggca ggtgaagtcc gacacggtcg gcatcccgct cgagggggtc
      481 gaggttcgcg tactcgactc gggcgagatc gtgctgcgct gcccgggcct tttcgtcgag
      541 tactaccgga acgaggcggc cacgcgcgaa gcgaaggacg ccgagggctg gtaccacacg
      601 ggcgacgcgg ggtacttcga cacgagcggg cacctcaaga tcatcgatcg cgccaaggac
      661 gtgggcaagc tcctcgacgg aacgctcttc gcgccgaaat acctcgagaa caagctcaag
      721 ttcttcgccg caatcaagga ggccgtcgcc ttcggcgatg gccggcccga ggccaccgcc
      781 atgatcaaca tcgacatgca ggcggtgggt gactgggcgg agcgtcgcgg catcgcctat
      841 tccggttacc aggatctctc ggcgaagccc caggtctacg agctcatccg cgagtgcgtc
      901 gagaaggtca acgccgacct ctcggccgat cccaagctcg gcggcagcca gatccaccgc
      961 ttcgtcgtgc tgcacaagga gctggacgcc gacgacggcg agctcacgcg cacgcgcaag
     1021 gtgcgccggc gattcatcgc ggagaagtac gagcccatcg tccacgcgct atacggggac
     1081 atggaccatt gctgggtcga ggcccaggtc cgcttcgagg atggccgcac gggcaccgtg
     1141 cgtgcgcagc tcgccgttcg cgaggcgaag accttcccgc cgcaggcggc aaggttggca
     1201 gcttgaggcc tgcggcatga gcgacaccga acgccagttc ggcgaagtga tcctcaagct
     1261 cgagggcatt tccctcgcct tcggcggcgt gaaggcgctc accgacatca ccttcgacgt
     1321 gcgggagcac gagatccgcg ccatcatcgg ccccaacggc gcgggcaaga gctcgatgct
     1381 caacgtgatc aatggcgtct accagccgca gcagggcaag gtgaccttcc gcggcgagac
     1441 gcgcgaggcc atggagccgc accacgccgc gcagctgggc atcgcgcgaa ccttccagag
     1501 cctggcgctg ttcaagggca tgtccgtgct ggacaacctg ctggccgggc gcaacctgat
     1561 gatccgctcg aacatcttcc ttcaggcgct gcgcgtgggc ccggcgctgc gcgaggaggt
     1621 gcagcaccgc aagcgcgtcg aggaaatcat cgacttcctc gagatccagc acgtgcgcaa
     1681 gactcccgtg gggcggctgc cgtacggcct gcagaagcgc gtcgacctgg gccgtgcgct
     1741 cgccgcggaa ccgaaggtgc tcctgctcga cgagccgatg gccggaatga acgtcgagga
     1801 gaagcaggac atgtgccgct tcatcctcga cgtgaacgac gagttcggca ccaccatcgt
     1861 gctgatcgag cacgacatgg gcgtggtcat ggacatctcc gatcgcgtgg tggtgctcga
     1921 ctacggcaag ctgatcggcg acggcacccc cgacgaggtg cgcgccaacc aggcggtgat
     1981 cgacgcatac ctgggagtaa agcattgaac ctgttccaga cgaagtacat gacctacgcg
     2041 ctgttcgtcc tgctcgccat gggggcggtg gcgcctcttt tcgccgcgga cacgcgcctg
     2101 ccgcagtggc tcgaggtggt gctgggcggg ctgatgtcgg gcgtgctcta ttcgctcgtg
     2161 gcgctggggc tcgtgctcat cttcaaggcc tccggcgtgt tcaactttgc gcagggggcg
     2221 atggtcctct ttgccgcctt gtcgctggtg cgcgtcatgg agaaggtgcc ctttgcggtc
     2281 gcgctcgtga tcaccgtggt gatcatggtg gcggttgcct ggctcatcga gcgcctggtg
     2341 ctcaggccgc tcgtcaacca ggagggcatc gtccttttca tggcaacgct cgggatcgcc
     2401 tatttcctag acgggctcgg ccagacgatc tggggaagcg acgtgtatac gctcgacgtc
     2461 ggcatgccga aggagcccaa ggtgctgctc gagaacgtct tcgagggcgg gatcctggtc
     2521 ggcatggagg acgtgatcgc cgcgttcatc tcggccgcgc tggtcgccct gctcgcgctc
     2581 ttcttccaga agacgggcac cgggcgcgcg cttcgtgccg tggccgacga ccaccaggcc
     2641 gcgcagtcca tcggcattcc gctcaaccgc atctgggtca tcgtgtggag cgtgtcggga
     2701 atcgtggcgc tcgtggccgg gatcatctgg ggctccaagc tcggcgtgca gttctcgctg
     2761 cagctcgtcg cgctgaaggc gctgcccgtg gtgatcctgg gcgggttcac ttccatcccg
     2821 ggcgcgatca tcggcgggct catcatcggg ctgggcgaga agctttccga ggtctatctc
     2881 gggccctacc tcgggggcgg aatcgagaac tggttcgcgt acgtgctcgc gctggtcttc
     2941 ctgctgttcc ggccggaggg actcttcggc gagcggcaca tcgaccgggt atagagatga
     3001 tttatcgcga gacgggacag tacaagtcct cctacgaggc cgaccagcag cttttcccca
     3061 tcctccagga caaggtcttc atcctgctgg tgctcgcgtt cgtgttcggc gccgtgcctt
     3121 tcctcgcgag cgagtacctg ttccgcgcca tcctcatccc gttcctcatc ctcacgctcg
     3181 cggccatcgg cctgaacatc ctggtcggct tctgcgggca ggtctcgctg gggaccggcg
     3241 gattcatggc ggtgggcgcg tacgcagcct acaaccttgc gatccggctg ccggacctga
     3301 acctgctcgt cgtcttcctc ctcgccgggg tggcggccac ggtggtgggc gtgctcttcg
     3361 ggctgccgag cctgcgcatc aagggcttct acctcgccgt ggccacgctt gccgcgcagt
     3421 tcttcctcga ctgggcgttc gcgcgcatcg gctggttcac caacaacgcg ccgtcgggat
     3481 cggtcacggc gcccgcgatc acgatcttcg ggtacacgtt cacctcgccg gtccagaagt
     3541 acctgctggt gctctccttc gtctgcgtct tcgccctggt cgcgcgcaac ctaacgcgcg
     3601 gggcgatcgg gcgctcgtgg atggccatgc gcgacatgga cgtggcggcg gaagtcatcg
     3661 gcatccggcc cctctacgcc aaggtgacgg cctttgcggt cagctcgttc ttcgtgggcc
     3721 tggcgggcgc cctgtgggcg ttcgtctacc tcggcgcctg ggagcctctc gctttcgacg
     3781 tgaaccgttc gttccagctg cttttcatgg tcatcatcgg cgggctgggg tcgatcctgg
     3841 gttccttcct gggggcggcg ttcatcgtca tcctgccgct cgtgctcaac caggcgccgg
     3901 gatggctcgg catggatctc tcgaccgccc tgatctcgca catcgaattc atgatcttcg
     3961 gggcactgat catcttcttc ctgattgtcg aaccccacgg actggcgcgg ctgtgggcca
     4021 tcggcaagga aaagctgcgg ctctggccgt ttccccattg acccggcccc gagcgccttt
     4081 gcaacacgcc gtcgatccaa ccgcaccatc aatacgagga gtatggagat gaaagcgagc
     4141 atcaaacgga aggccgccgc tgcggccatc gccgtggcgg gcgtcctggg attcgcgcag
     4201 gaagccgtcg cggccgacga gatcttcgtc ccagtgctgg tctatcgcac cggcgcctac
     4261 gcgcccaacg gcgtgccctg ggcggacgga ttcgtggact acctaaagct cgtcaacgag
     4321 cgtgacggcg gcgtgaacgg cgtcaagatc gcgtacgagg agtgcgaaac cggctacgcg
     4381 accgaccgcg gggtcgagtg ctacgagcgc ctgaagagca agaagccggt cgccgtgaat
     4441 ccgctttcca cgggcatcac cttcgcgctc accgagaagg ttgccgccga caagatcacc
     4501 ctcatgacca ccggctacgg gcgttccgag tccgccgacg gcagcgtctt cgcgtggaac
     4561 ttcccgctgc tgggcaccta ctggtccgcg gccgacatgc tcgtgcagca cgtggccaag
     4621 aagggcagcc tcaagggcaa gaagatcgcg ctcgtctacc acgactcgcc ctacggcaag
     4681 gaaccgatca agatcctcga ggagcacgcg aagaagcacg ggttcacgct gttgaagctc
     4741 ccggtgacgc accccggcgt ggagcagaag gcgacctggc tgcagatccg ccagcagcgt
     4801 cccgattatg tcttcctctg gggctggggg gtcatgaact cgacgtcgat caaggaggcg
     4861 atcgccgtct cctatccgcg cctgcagatc ttcggcgtgt ggtggtcctc cgccgagccg
     4921 gacgtgctgc ccgccgaagc cgccgccaag ggctacaacg gcatcacgct gggccactcc
     4981 aacgagcgca cgctgccggt gcacaaggac atcgccaagc acctctacga caagggcaag
     5041 ggcacggccg ccaagaagga gctggtcggc gacgtgctct aca
//
LOCUS       DASUEV010000372         9768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacteraceae bacterium isolate SMAG_U1362
            k141_568641, whole genome shotgun sequence.
ACCESSION   DASUEV010000372 DASUEV010000000
VERSION     DASUEV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961514
            Sequence Read Archive: SRR25158404
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacteraceae bacterium (soil metagenome)
  ORGANISM  Usitatibacteraceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:49:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,358
            CDSs (total)                      :: 2,331
            Genes (coding)                    :: 2,327
            CDSs (with protein)               :: 2,327
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Usitatibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568641"
                     /isolate="SMAG_U1362"
                     /isolation_source="soil"
                     /db_xref="taxon:3022879"
                     /environmental_sample
                     /country="China"
                     /lat_lon="41.01583333 N 122.405 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFV55_11740"
     CDS             <1..420
                     /locus_tag="VFV55_11740"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX4945018.1"
                     /translation="KAGLAKPRIIVCGLNPHNGDNGAFGREEIDIIGPGVELARSRGF
                     PADGPFPADTVFVRATRKGGGYDGVVTMYHDQGQIAMKLMGFERGVTVQGGLPIPICT
                     PAHGTAYDIAGRGIASDGAMKNAFELACRMGEGAAGF"
     gene            complement(427..2286)
                     /gene="pepF"
                     /locus_tag="VFV55_11745"
     CDS             complement(427..2286)
                     /gene="pepF"
                     /locus_tag="VFV55_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX4945019.1"
                     /translation="MNSTLPLVAVAIALHATLAPAAERPEDRWNLADVYASPQAWQQD
                     AEKLEAQLREFTACRGHLGDSAKRFKACADLQADIGKRYARLDLYAQELFAEDTGVAA
                     TLELRQRSGVLATRVDEATSFVRPEILKIGKAKVAAFLATDKSLAIYRQPLDEILRAA
                     PHTLDAKGEAILATLGLAAGSAAATFEILSSADLPWPRVMLSDGREVTVDQSAYGELR
                     ELPDREDRKKVFDAFWGRWKDYERTFGVTFYENLKQDTAYAKVRRYRDSISRSLDRNR
                     LPPAVYDTLLAETRANLPTLHRYFRLRAKMLGIPAGEMRYYDIYPPLVKSDLRYPLAQ
                     GKRMTLEAVKPLGAEYASVMASGFASRWMDAYPRPHKNPGAHMAGTAYDVHPYVLMNY
                     TDNYESVTTLAHEWGHAMHSFLSNRNQPFVTSDYATFIAEIASTLNEALLLDNVLKGA
                     KSDEERLLYLGSALEGLRATFFRQAMFAEFEREVHARVDKGESLTGESLTRIYGDILR
                     RYHGEAEGVVKIDDLYTVEWAYIPHFYSAFYVFQYATSIAASSLLADAILGGEAGARE
                     RYLKLLASGSSDYPYELAKAAGVDLATPAPYRALVARMNRIMDEIEAILARRK"
     gene            2454..2852
                     /locus_tag="VFV55_11750"
     CDS             2454..2852
                     /locus_tag="VFV55_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX4945020.1"
                     /translation="MAKIIHTMVRVLDLDRSLRFYRDAFGLEEVHRLDFPTFALAYLR
                     NGENDFEVELTLNKDRKEPYTHGDGYGHFAVCVDDVARERERLTQVGCVPGDVKEFKD
                     GAGALIARYFFVQDPDGYKIEVLERHGHYR"
     gene            2928..3542
                     /locus_tag="VFV55_11755"
     CDS             2928..3542
                     /locus_tag="VFV55_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX4945021.1"
                     /translation="MDSNRLTLSAGHGHTPAQPRAQIALTRREFLKGTGILAGTIAAS
                     SILSALAPSRVWALELKALSSAQGDAILQMGKVLYPHKGLPDAVYALLARDLDGAAGK
                     DPKTAKMLGEGITALDKAAGGSFVAATDAKKLAAVKSLEGTPFFGTVRGQCITSLYDN
                     EMAFAHFGYPGPSWDKGGYVLRGFGDLKWLPDPPASASPAPYKA"
     gene            3578..5146
                     /locus_tag="VFV55_11760"
     CDS             3578..5146
                     /locus_tag="VFV55_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX4945022.1"
                     /translation="MAKFELNDDSVVVIIGSGAGGGTLANELCQKGVKVVLLEAGKRE
                     SPATFINDEWKSFGQLAWLDKRTTSGTWRIAKDFAGLPAWICKTVGGTTTHWAGASLR
                     LHEHEFRARTVYGDVKGATLLDWPLTLKDLEPFYAKAEDKLGVTRTHDVPGLPGNNNF
                     KVMYAGAKKAGYKDCNTGHMAINSRPRADRGSCLQIGFCFQGCKSGAKWSALYTEIPA
                     AEKTGKLDLRPQAHVLRIEHNDAGKVTGVVYRDADGKDQRQKARVVCVAGNSIETPRL
                     LLLSASAKYPDGLANSSGQVGRNYMRHMTGSVYAVFKEPVNMYRGTTMAGIITDEAGN
                     NPKRGFVGGYEMETLSLGLPFMAAFLDPGAWGPDFTYFLDNYTRMAGMWLVGEDMPRA
                     TNRVTLNAAEKDQFGLPVPNVHFDDHDNDVAMRNHAYAAGEKVYLAAGAVKTFRTPPY
                     PSTHNLGTCRMSTKPGDGVCNKWGQTHDIANLFISDGSQFTTGGAENPTLTIVTLAIR
                     QAGYLAEQLAKRTI"
     gene            5210..5542
                     /locus_tag="VFV55_11765"
     CDS             5210..5542
                     /locus_tag="VFV55_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX4945023.1"
                     /translation="MCEIYCKADPILYESRTRSLRMRGVLTTIRLENLFWEVLTEMAG
                     REGMTTNQLVAKFHDEVVAWRGESVNFASFLRVCCLRYLSVAARQEPGGPPRQAVRAA
                     ATGLRSVG"
     gene            5603..6388
                     /locus_tag="VFV55_11770"
     CDS             5603..6388
                     /locus_tag="VFV55_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX4945024.1"
                     /translation="MTTTVPEAWRRWIPTAITVALIVAMLAYGPIHQPADYHDFADRR
                     AALGVANAADVVSNIGFALAGLWGLAAWSAWMRLPDRPRSSRGYAVFFVALVLTGLGS
                     AWYHLLPDNGRLVWDRIPIALACAGLLAAVHADTHSPGQHAAIPVALVAAAFASVWWW
                     SHTGVDGPGDLRPYLLLQVLPLLLIPVWQALYGARREERLAFGIAIALYVGAKAAELG
                     DHAIFDATGFLSGHTLKHLLATAAGAVVAADYRRRAAEAMRSK"
     gene            6508..6972
                     /locus_tag="VFV55_11775"
     CDS             6508..6972
                     /locus_tag="VFV55_11775"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX4945025.1"
                     /translation="MIDTPQITQTTIQQAAVIRLTIPREEIRNVMGPGIGEVIDAAQA
                     QGIGPAGPVFSHHFRMDPATFDFEVGVPVTAPVTPVGRVKPGHLPAATIARTVYHGPY
                     EGLGAAWGEFEAWLKANGHALAPNLWECYVAGPESSPDPANWRTELNRPLAR"
     gene            complement(7002..7457)
                     /locus_tag="VFV55_11780"
     CDS             complement(7002..7457)
                     /locus_tag="VFV55_11780"
                     /inference="COORDINATES: protein motif:HMM:NF020551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtaL-like protein"
                     /protein_id="HEX4945026.1"
                     /translation="MKFEHIIQVNDLLNPLVEPLSREEIWEGLVESVEAPTRFLPGID
                     TCRILWREGDKLCRELHFGHAVVHDTVRLTPGHRIAIDFDPSPEVPALKRTMTIEEPI
                     EGEYIVRFRYERSPQGHEPLAPAFASVLEAAWLQADIDLIAQIRRDRGG"
     gene            7633..8115
                     /locus_tag="VFV55_11785"
     CDS             7633..8115
                     /locus_tag="VFV55_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX4945027.1"
                     /translation="MARLHINGRVRDVQVEPETPLLWVIREQVGLTGTKYGCGVAQCG
                     ACSVHINGQVQRSCAVPVGSLKPGDRIVTIEGLSKGASHPVQKAWAQVDVPQCGYCQS
                     GQIMAAASLLAKNPDPSDADIDEAMTNICRCGTYQRVRAAVHVAAELAAGKAKAKARA
                     "
     gene            8133..>9768
                     /locus_tag="VFV55_11790"
     CDS             8133..>9768
                     /locus_tag="VFV55_11790"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX4945028.1"
                     /translation="MTTRNRKSVPDLSRRQFIVRSAAAGGGLALGFHLPFGIGAAEAA
                     SPAANGTEINAWVMIRADDTCVVRIARSEMGQGTLTGLAQLVAEELECDWKKVTTENI
                     TAGRNYAAKRTWGDMSTGGSRGIRTSQDYVRRGGATARLMLLQAAAERWNVPVSELTV
                     ADGVITHAASKRTTTYGKVAAAAAKLPAPDPKGVTLKDPKLWTIAGQPLKRLDTADKL
                     DGRKVYAIDVKLPGMLNAAIKACPVFGGKLVSYDEAAILKRPGVKGVVRVNNFTVAVV
                     ADTWWRAKSALEALPIVWDEGPGAGQSSALIDEHLRTGLSAAEAYQSRVIGDAPKAIE
                     AAAKKVEAVYSTPFLAHATMEPMNCTARITATKGEVWVATQNAEASLAALSEESGLPL
                     AACEVYRHDLGGGFGRRGGNQDYTRQAVAIAKQFPGKPVKLIWSREEDMGHDFYRPIS
                     VCRLSAGLDEKGELTGLHARISGQSINAFVNPAMVATGKDERQVQGWYEKGPVAQLGY
                     TFPNLLIEYAMRNTHVPVGPWRGVNTNQNGIYMECFI"
BASE COUNT         1664 a         3245 c         3357 g         1502 t
ORIGIN      
        1 aaggcggggc tcgcgaagcc gcgcatcatc gtgtgcggac tgaatccgca caatggcgac
       61 aatggcgcgt tcgggcgcga ggagatcgac atcatcggtc ccggggtcga gctggccaga
      121 agccgcggtt tccccgccga tggcccgttt ccggccgaca cggtcttcgt gcgcgccacg
      181 cgcaagggtg gcggctacga cggcgtggtc accatgtacc acgaccaggg gcagatcgcg
      241 atgaagctga tgggcttcga gcgcggcgtc accgtgcagg gcggtctccc catcccgatc
      301 tgcacgccgg cgcacggcac ggcctacgac atcgcgggcc ggggtatcgc gagcgatggc
      361 gccatgaaga acgccttcga gctggcctgc cgcatgggcg aaggagcggc cggattctga
      421 atcggcctac ttgcgccgcg cgaggatcgc ctcgatctcg tccatgatgc ggttcatgcg
      481 cgcgacgagc gcccggtacg gggcgggcgt ggcgaggtcc acgcccgccg ccttcgccag
      541 ctcgtagggg taatcggagc ttcccgaggc gaggagcttc aggtagcgct cgcgcgcgcc
      601 cgcctcgccc ccgagtatcg cgtccgcgag gagcgaggag gccgcgatcg aggtcgcgta
      661 ctggaagacg tagaacgcgc tgtagaagtg cgggatgtac gcccactcga cggtgtagag
      721 atcgtcgatc ttcaccaccc cctcggcttc gccgtggtac cggcgcagga tgtcgccgta
      781 gatccgggtc agcgattcgc cggtgaggga ttcgcccttg tccacgcgcg cgtgcacctc
      841 gcgctcgaat tccgcgaaca tggcctggcg gaagaaggtg gcgcgcaggc cctcgagcgc
      901 ggaacccagg tagaggaggc gctcctcgtc cgacttcgcg cccttgagca cgttgtcgag
      961 gagcagcgcc tcgttcagcg tggaggcgat ctcggcaatg aaggtggcgt agtcggacgt
     1021 cacgaaaggc tggttcctgt tggaaagaaa cgagtgcatc gcatggcccc actcgtgggc
     1081 gagcgtcgtg accgactcgt agttgtccgt gtaattcatg agcacgtagg ggtgcacgtc
     1141 gtaggccgtt cccgccatgt gcgcgcccgg gttcttgtgc ggccgcggat aggcgtccat
     1201 ccagcggctg gcgaaaccgc tcgccatcac cgaggcgtac tcggcgccca ggggtttcac
     1261 cgcctccagc gtcatgcgct ttccctgcgc gaggggatag cgcaggtcgc tcttcaccag
     1321 cggcgggtag atgtcgtagt agcgcatttc gcccgcgggg attcccagca tcttcgcgcg
     1381 caggcggaag tagcgatgca gcgtcggcag gttggcccgc gtctccgcga ggagcgtgtc
     1441 gtacacggcg ggcggcaggc ggttgcggtc gagcgagcgg ctgatcgagt ccctgtagcg
     1501 ccgcaccttc gcgtaggccg tgtcctgctt caggttctcg tagaaggtga cgccgaaggt
     1561 gcgctcgtaa tccttccagc gtccccagaa cgcgtcgaac accttcttgc ggtcctcgcg
     1621 gtcgggcagc tcgcgcagct ccccgtaggc ggactggtcc accgtcactt cacgcccgtc
     1681 ggagagcatc accctgggcc aggggaggtc ggcgctggag aggatctcga aagtggccgc
     1741 ggcggacccg gcggcgagac cgagggtggc caggatggct tcgcccttcg cgtcgagcgt
     1801 atggggtgcg gcgcgcagga tctcgtcgag gggctggcga tagatggcga gggacttgtc
     1861 cgttgccagg aaagccgcga ccttcgcctt gccgatcttc aggatctcgg ggcgcacgaa
     1921 ggaggtcgcc tcgtcgacgc gggtggcgag cacgcccgag cgctggcgaa gctccagggt
     1981 tgcggcgacg cccgtgtcct cggcgaacag ttcctgggcg tagagatcga ggcgggcgta
     2041 gcgcttgccg atgtcggcct gcaggtcggc gcaagccttg aagcgcttcg ccgaatcgcc
     2101 gaggtggccg cggcaggcgg tgaattccct gagctgggcc tcgagctttt cggcgtcctg
     2161 ctgccaggcc tggggcgagg cgtagacgtc ggcgaggttc cagcggtcct cgggcctttc
     2221 ggccgcgggg gcgagggttg catggagggc gatggccacg gcgacaaggg ggagggttga
     2281 attcatcggc gggagaatgc ctgaaaagcg ccgcgccatg tgtcacggtg cattgccggt
     2341 caccggcgca ggttcggaat ccggcacggc cggcgccatt tagcgggcta ttaccaaggc
     2401 gtgcggattg aaggctagat ttcgggcgac gctcggatgg aaggcgcccg aagatggcca
     2461 agattatcca caccatggtc cgcgtgctcg acctcgacag gtcgctgcgc ttctatcgcg
     2521 atgccttcgg tctcgaggag gtgcaccgtc tcgattttcc gacgttcgcg ctggcgtatc
     2581 tcaggaacgg cgagaacgac ttcgaggtcg agctcacgct caacaaggac cggaaggaac
     2641 cctacacgca tggcgacggc tacggccatt tcgccgtctg cgtcgatgac gtggcgcgcg
     2701 aacgcgagcg gctcacgcag gtcgggtgcg tgcccgggga cgtcaaggaa ttcaaggatg
     2761 gcgccggcgc tctcatcgcg cggtacttct ttgtccagga tcccgacggc tacaagatcg
     2821 aggtgctcga gcgccacggc cactaccgct agccgggcgc gcgcgaccga acgatgcgcg
     2881 gcaaacgcga ggggggaggc ccccgcgttc caacatagga ggagaagatg gactcgaacc
     2941 gcttgaccct ctcggccggc cacggccaca ccccggcgca gccgcgggcg cagatcgcgc
     3001 tgacacggcg cgagtttctc aagggaaccg gcatcctggc cggaacgatt gcggcctcct
     3061 ccatcctgtc ggcgctcgcg ccgagccgcg tttgggcgct cgaactcaag gcgctctcct
     3121 cggcccaggg cgacgcgatc ctgcagatgg gcaaggtgct ctacccgcac aaggggctgc
     3181 cggacgcggt ctatgcgctc ctcgccaggg atctcgacgg cgcggcgggc aaggatccga
     3241 agaccgcgaa gatgctcggc gagggcatca ccgcgctcga caaggcggcc ggcggaagtt
     3301 tcgtcgcggc gaccgacgcg aagaagctcg cggcggtgaa gtcgctcgag ggcacgccat
     3361 tcttcggcac cgtgcgcggg caatgcatca cgtcgctcta cgacaacgaa atggcgttcg
     3421 cccacttcgg ctatcccggc ccctcgtggg acaagggggg ctatgtcctg cgcgggttcg
     3481 gcgatctcaa gtggctgccc gatccgcccg cgtcggcgag ccctgcaccc tacaaggcct
     3541 gagttccggt caaccagacg cgagggagga acggatcatg gcgaagttcg aactcaacga
     3601 cgattccgtg gtggtcatca tcggttccgg cgccggcggc ggcacgctgg cgaacgaact
     3661 gtgccagaaa ggcgtcaagg tcgtgctgct cgaagccggc aagcgcgagt ccccggcgac
     3721 cttcatcaac gacgagtgga agtcgttcgg gcagctcgcc tggctggaca agcgcaccac
     3781 gtcgggaaca tggcggatcg ccaaggattt cgcgggcctg ccggcatgga tctgcaagac
     3841 ggtcggcggc acgacgaccc actgggcggg cgcgtcgctg cgcctgcacg agcacgaatt
     3901 tcgcgcgcgc accgtctatg gcgacgtcaa gggagcgacg ctcctcgact ggccgctcac
     3961 gctgaaggac ctcgagccgt tctacgccaa ggccgaggac aagctgggcg tgacgcgcac
     4021 ccacgacgtg cccggcctgc ccggcaacaa caacttcaag gtcatgtatg ccggcgcgaa
     4081 gaaggcgggc tacaaggact gcaacaccgg ccacatggcg atcaacagcc ggccgcgcgc
     4141 ggatcgcggc tcctgcctgc agatcggctt ctgcttccag ggctgcaagt ccggcgccaa
     4201 gtggtctgcg ctctataccg agattccggc tgccgagaag accggcaagc tcgaccttcg
     4261 cccgcaggcg cacgtgctcc ggatcgagca caacgatgcc ggcaaggtca cgggcgtcgt
     4321 gtaccgggat gccgacggca aggaccagcg gcagaaggcg cgcgtggtgt gcgtcgccgg
     4381 caactcgatc gagaccccgc ggctgctgtt gctctccgcc tccgccaagt accccgacgg
     4441 gctggcgaac agttccggcc aggtcggcag gaactacatg cgccacatga cgggatcggt
     4501 gtacgcggtg ttcaaggagc cggtgaacat gtaccgcggc acgacgatgg ccggcatcat
     4561 caccgacgag gcgggcaaca acccgaagcg cggcttcgtc gggggctacg agatggaaac
     4621 gctgtcgctc ggcctgccgt tcatggcggc gttcctcgac cccggtgcct ggggtccgga
     4681 cttcacgtat ttcctggaca attacacgcg catggccggc atgtggctcg tcggcgagga
     4741 catgccccgc gcgacgaacc gggtcacgct caatgccgcc gagaaggacc agttcggcct
     4801 gccggtgccc aacgtccatt tcgacgacca cgataacgac gtcgcgatgc gcaaccatgc
     4861 ctacgccgcc ggggaaaagg tgtatctggc ggcgggagcg gtgaagactt tccgcacgcc
     4921 gccctatccg tccacgcaca atctcggcac ctgccgcatg agcacgaagc cgggcgacgg
     4981 cgtgtgcaac aagtggggac agacgcacga catcgccaac ctgttcatct cggacgggag
     5041 ccagttcacg accggcggcg cggaaaaccc gacgctcacc atcgtgacgc tcgcgatccg
     5101 gcaggccggc tatttggcgg aacagctggc aaagcgcacc atctgaggca cgaacgcggc
     5161 gtccacagcc gcgaggtcgg tccggcgcgc ttttccggag ggcggataca tgtgcgagat
     5221 ctactgcaag gccgatccga tactctacga gtcgcgcacg cggtccctcc ggatgcgcgg
     5281 cgtcctgacg acgatccgcc tggaaaacct cttctgggaa gtgctgaccg aaatggccgg
     5341 ccgcgaggga atgaccacga accagctcgt cgcgaaattc catgacgagg tagtggcctg
     5401 gcgcggcgag agcgtcaatt tcgcctcctt cctgcgggtc tgctgcctgc gctacctgtc
     5461 ggtggctgcc cggcaggaac cgggcggccc gcccaggcag gccgtgcggg cggcggcgac
     5521 cggattgcgg tcggtcggct agatccggag ggatgccggt cacgtcaccg aaccgggtgg
     5581 ccattcatcc agtgacgcgc gcgtgacgac aacggttccc gaggcgtggc gccggtggat
     5641 tccgaccgcc atcacggtcg ccctaattgt cgccatgctg gcttacggcc cgatccacca
     5701 gcctgcggac tatcacgact tcgcagaccg ccgcgcagcc cttggcgtgg cgaatgccgc
     5761 cgacgtcgtg tcgaacatcg gcttcgccct cgccggcctc tggggccttg cggcgtggtc
     5821 ggcgtggatg cgccttcctg atcggccacg ctcatcgcgc ggctatgcgg tgttcttcgt
     5881 cgcgttggtg cttacgggcc tgggctccgc ctggtatcac cttcttcccg acaacggccg
     5941 gctggtctgg gaccgcatcc cgatcgcgct cgcctgcgcc ggcctgctgg cggccgtcca
     6001 tgccgatacg cattcaccgg gccagcatgc cgcaattccg gtcgcgctcg tggcggcggc
     6061 gttcgccagc gtgtggtggt ggtcgcatac cggcgtggat ggcccgggcg acctgcgtcc
     6121 gtacctgctg ttgcaggtac ttcccctgct gctcataccg gtatggcagg cactctacgg
     6181 cgcccggcgc gaggagcgcc tcgcctttgg catcgccatc gcgctctatg tcggcgcgaa
     6241 agccgcggag ttgggcgatc acgcgatctt cgacgcgacc ggcttcctga gcgggcacac
     6301 cctgaaacac ctgctcgcga ccgcggcggg agccgttgtc gcggcggact accgacggcg
     6361 cgccgcggaa gcgatgcgat cgaagtagaa ccgagctgtc gaaattgcgc ccccttgttc
     6421 gactaggtgt tggaggcagg acatgcgcct cgcacgaggt cgccccgctg acggttccgc
     6481 gaacgcacaa cacaggagac tgcacccatg atcgacacgc cgcagatcac gcagaccacc
     6541 atccagcaag ccgccgtcat ccgcctgacg attccgcgcg aggagatccg caacgtcatg
     6601 ggcccgggaa tcggcgaggt gattgacgcc gcccaggcac aaggaatcgg tcccgccggc
     6661 ccggtgttct cgcaccattt ccggatggat ccggccacct tcgacttcga ggtcggggtt
     6721 cccgtgacag cgcccgtcac gccagtcgga cgcgtgaagc cgggccatct gcccgccgcg
     6781 acgatcgcgc gcaccgttta ccatggcccc tacgaaggcc tgggcgccgc ctggggcgag
     6841 ttcgaggcct ggctcaaggc gaacgggcat gcgctcgccc cgaatctctg ggagtgctat
     6901 gtcgccggcc ccgaatcgag ccccgacccg gccaactggc gcacggaact caaccggcct
     6961 ctcgcccgct gacgactggg gccatcgcgg gagcgggcgt atcaaccccc ccgatcgcgc
     7021 cggatctgcg caatgaggtc gatatccgcc tgcagccacg ccgcctccag gacggacgca
     7081 aatgccggtg cgagcggctc gtgcccctgc ggcgagcgct cgtaccggaa gcgcacgatg
     7141 tactccccct cgatcggctc ctcgatggtc atcgtgcgct tgagcgccgg aacctcgggt
     7201 gaagggtcga agtcgatggc aatccggtgc ccgggcgtga ggcgcacggt gtcgtgcacc
     7261 accgcgtggc cgaagtgcag ctcgcggcac aacttgtcgc cttcgcgcca gaggatgcgg
     7321 caagtatcga tacccggcag gaatcgcgtc ggcgcctcga cgctttccac gagcccctcc
     7381 cagatctcct cgcgggaaag gggttccacc agcgggttga gcaggtcgtt gacctggatg
     7441 atgtgctcga acttcacccg ggcatcttgc cctaacgcac gctgaagcgc gcgttgcagg
     7501 gccgcccgaa cgcgatgccg ccaacccggt caaggcgcag tgcgcacaac ggcggtcatt
     7561 gacctgcgcg gatcccgcga cgagaatcgc ccggctgcag gccaccattc caacgagccg
     7621 gggagaaaag ccatggcgag actccacatc aacggcaggg tgcgcgacgt gcaggtcgag
     7681 ccggagacgc cgctgctctg ggtcatccgc gagcaggtcg ggcttaccgg gacgaaatac
     7741 ggctgcggcg tcgcgcaatg cggcgcgtgc tcggtgcaca tcaacgggca ggtgcagcgc
     7801 tcgtgcgcgg ttcccgtggg cagcctcaag cccggggacc gcatcgtgac catcgaggga
     7861 ctctcgaagg gcgcctcgca tccggtgcag aaggcgtggg cgcaggtgga cgtgccgcag
     7921 tgcggctact gccagtcggg ccagatcatg gctgccgcgt cccttctcgc gaagaacccg
     7981 gatccctcgg acgccgacat cgacgaggcg atgaccaaca tctgccgctg cgggacctac
     8041 cagcgggtgc gggcggcggt gcacgtcgcg gcggaactcg ccgccggcaa ggcgaaagcg
     8101 aaggccaggg cctgaggcgc gaggtgacgg acatgacgac ccgcaaccgc aagagcgtcc
     8161 cggacctctc ccgccgccag ttcatcgtgc gcagcgccgc cgccggcggt gggctggcac
     8221 tcggattcca cctcccgttc gggatcgggg ccgccgaggc cgcgagtccc gccgcaaacg
     8281 gcaccgagat caacgcctgg gtgatgatcc gcgccgacga tacctgcgtg gtgcgaatcg
     8341 cgcgctccga gatggggcag ggcacgctca ccggcctcgc gcagctcgtg gccgaggaac
     8401 tcgagtgcga ctggaagaag gtcaccacgg agaacatcac cgccgggcgc aactacgccg
     8461 ccaagcgcac gtggggcgac atgagcacgg gcggcagccg tggcatccgc acctcccagg
     8521 actacgtgcg gcgcggtggc gccaccgccc gcctcatgct gctgcaggcc gcggccgaaa
     8581 gatggaatgt gcccgtgtcc gagctcaccg tggccgacgg cgtcatcacc catgctgcct
     8641 cgaagcgcac cacgacctac ggcaaggtgg ccgccgccgc cgcgaaactc ccggcgccag
     8701 atcccaaggg cgtgacgctc aaggacccga agctgtggac gatcgccggc cagccgctca
     8761 agcgcctgga caccgccgac aagctcgacg gccgcaaggt ctacgcgatc gacgtgaagt
     8821 tgccgggcat gctcaacgcg gccatcaagg cctgtcccgt gttcggcggg aagctcgtga
     8881 gctacgacga ggcggcgatc ctgaaacgtc ccggcgtgaa gggcgtggtc agggtgaaca
     8941 acttcaccgt ggccgtcgtg gccgacacat ggtggcgcgc gaagagcgcg ctcgaggcgc
     9001 tgcccatcgt ctgggacgag ggccccggag ccggtcagtc gagcgccctg atcgacgagc
     9061 accttcgaac cggcctcagc gcggccgagg cctaccagag ccgcgtgatc ggggatgcgc
     9121 ccaaggcgat cgaggctgcc gcgaagaagg tcgaggcggt gtattcgacg ccgttcctcg
     9181 cccacgcgac gatggagccg atgaactgca ccgcgcgcat taccgccacg aagggcgagg
     9241 tgtgggtggc cacgcagaat gcggaggcct cgcttgccgc cctttccgag gaatcggggc
     9301 tgcccctcgc ggcctgcgaa gtctatcgcc acgacctggg cggcggattc ggccggcgcg
     9361 gcggcaacca ggactacacg cgccaggccg tggccatcgc gaagcagttt cccggcaagc
     9421 ccgtgaagct catctggagc cgcgaggaag acatggggca cgacttctac cggcccatct
     9481 ccgtgtgcag gctctccgcg ggcctcgacg aaaagggcga gctcaccggg ctgcacgcgc
     9541 gcatctccgg ccagtcgatc aacgccttcg tgaatcccgc gatggtcgcc accggcaagg
     9601 acgagcgcca ggtgcagggc tggtacgaga agggcccggt cgcgcaactc ggctacacct
     9661 tcccgaatct cctcatcgag tacgcgatgc gcaacaccca cgtgccggtc ggaccgtggc
     9721 gcggcgtgaa cacgaaccag aacggcatct acatggagtg cttcatcg
//
